LOCUS       DAORVN010000001        13822 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_2049_length_13822_cov_3.341323, whole genome shotgun sequence.
ACCESSION   DAORVN010000001 DAORVN010000000
VERSION     DAORVN010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 13822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13822
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2049_length_13822_cov_3.341323"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3870)
                     /locus_tag="PKV23_00005"
     CDS             complement(<1..3870)
                     /locus_tag="PKV23_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HQX83840.1"
                     /translation="MLRMIGNRLRHTPSAWDDTQPISAEIFGLERSKQHARSLAESQI
                     TTDHPPRVYSIIDRLDDNATVLLAAYREICAALAAGNSITPAAEWLVDNYHLVEEQIL
                     QTRADLPPGFYRQLPKLAEGPLAGHPRIFGLVWAYVAHTDSHFNPATLTDFVNEYQLV
                     QPLTIGELWAVAISLRLILIENLRRISQRIMAARRGRETADKLADQVLDLGDKHANLA
                     RLFNEIDETAISKSFAVQLIQRLRDHGDVAGQALDWLKHKTDKLGYTFEAAVSDEHHR
                     QAAANVTVRNIVTSLRLIADVNWEVWFDSVSLVDQLLRTHSHYGDMDFASRTIYRTAI
                     EELSRGSNHSELAIARLAIDTATGAGVADEAVTPDPGYHLIGPGRAKFEPDLGFKPPL
                     LRRVRMAVRATGLAGYLGSVALLTGLTLLAGVSPLFQGETSYLLLIVLMLLALFPASD
                     VGIAIVNFAVTRLMDAAVIPGLALREGVPAQFRTLVVVPTLLISRDNIEELVDRLEVH
                     YLSNADGELYFALLTDWTDADTEHVPADATLLNVALEGIQRLNLRHNTDRFLLLHRSR
                     KWNPQEGKWMGWERKRGKLHELNRLLRGATDTTFCAIGGKLPPDIRFVLTLDSDTKLP
                     RDAARRLVGKLAHSLNQAKYDAQAGRVVEGYSVLQPRVTPSLPVGHYGSLFQRIFSSA
                     RGIDPYVFAVSDVYQDVFGEGSFAGKGIYDIDIFEAALAGKIPENTMLSHDLFEGVFA
                     RAALVTDIEVVEEYPERYAVAAARQHRWARGDWQLLPWMLGRRPNSKIPPLGLWKMAD
                     NLRRSLTPTATLGGLLLGWLFLPAGLAATWTAFIILTGVVPPLLPALSGAIPKRLPVT
                     VTSRIRSSLGDFLHAFVLAGANLVFLGHQAGLMADAVGRTLYRLAASRKNLLEWTTAA
                     QAESSHTPSVAGNYQVMSASLAAGVAAVAIAVFRADGIWFIAAPFAAAWFAAPAAAFW
                     ISRSPKLEDELESSPEDRKTLRLVARRTWRFFETFVTPAENMLPPDNFQEDPLPLVAH
                     RTSPTNIGLYLLSIASAREFGWLGLSDAVGRIEATLATARRMEKYNGHLYNWYETTTL
                     RPLEPRYVSTVDSGNLAGHLIALANYSASWMVEPAKVETSLEGIADALDVLAKDLSDI
                     PNDRQMLRPIRKQLESQLATLLRATEKAREAPEHILVRLIDFAVQAANIHATTTTYAA
                     ELDTTAGRQLLSWAAALRETVESHIKDASIGPDAAAGMKKRLEILIAEMRELALGMDF
                     GFLLEPRRLLLSIGYRVAEATRDESCYD"
     gene            complement(4086..5213)
                     /locus_tag="PKV23_00010"
     CDS             complement(4086..5213)
                     /locus_tag="PKV23_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018071034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HQX83841.1"
                     /translation="MNRIGVGLVGTGFMGKCHAMAFGAVKSVFGDVPHIDRVALCDVD
                     SSHARQKASEFGFNSATTNWRDLLADPTIHLISITSPNGMHREMAVAALEAGKHVWCE
                     KPMALTLADAEAMTAAAAKAKGQVTALGYAYLRNPALQYARQLITEGAIGEVFDFRGS
                     VDEDYMADPSLPWSWRLTRRDAGLGTLGDLTCHLVSLAHELVGEIGSLCAMADVVHDQ
                     RPLAGEAGKLAQVENDDIAHAVVRFRSGARGVLSSSRIAHGRKNGLRIEVHGSKGMLW
                     LDNERMNELNLYVADGPVAARGFRRILSSPAHPAYGKFCPAPGHGLGFNELKVIELAE
                     ILHAIQQTPSNHIDFARGLRIENVIHAFAQSSAARAWVSIE"
     gene            complement(5216..6007)
                     /locus_tag="PKV23_00015"
     CDS             complement(5216..6007)
                     /locus_tag="PKV23_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010659800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQX83842.1"
                     /translation="METPLVELKSVSKYFGSVIALKDVSFDVRPGEVHCLLGDNGAGK
                     STLIKVLSGVHIPDEGEILVGGTHTRFSSPKDTLDKGIATVYQDLALVPLMSVARNFF
                     LGREPLKKTWFLRQFDIEYANRTAYKELSDMGIQLRNPEQPVGTLSGGERQCLAIARA
                     IHFGAKVLILDEPTSALGVHQASVVLKLIVQSKARGIGVIFISHNVHHAYVVGDRFTL
                     LKRGKSAGTYQKNEISREQLLNLMAGGKELVDLEQEIIKLNNGKR"
     gene            complement(6007..7104)
                     /locus_tag="PKV23_00020"
     CDS             complement(6007..7104)
                     /locus_tag="PKV23_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010910925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQX83843.1"
                     /translation="MSLEVKDERVRAVPLLGRLLSRPELGAIAGTVLIFVFFGITAGD
                     SGLFSAKGIVNFLEVSAQLGSLAAAVALLMIAGEFDLSIGSMIGFAGIIIAIPPIMWD
                     WPVWASVLMAFGACAFVGYLNGLIVVHTGLPSFIVTLGSLFMLRGLTLGVTRGITGRT
                     QASGLHEYSAGDWITPLFAGQVFRTPIRWLADLGMIDKRADGLPAISGIPVSIIWWIG
                     LTLFFTWLLMRTRFGNWIFASGGEASAARNMGVPVARTKITLFVMTAAAAALFATIQV
                     LVTGSADTLRGNLKEFEAIIAVVIGGTLLTGGYGSAIGAMFGALIFGVVQMGIFYTGI
                     DTDWFKLFMGAMMVVAVLFNSYVRSRAMRSR"
     gene            complement(7165..8121)
                     /locus_tag="PKV23_00025"
     CDS             complement(7165..8121)
                     /locus_tag="PKV23_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010910924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HQX83844.1"
                     /translation="MFSLVKILKPMLGLLAGLTMLSAAAGIAAAEDTRVVFVTHGQSG
                     DPYWSVVKNGMDDAAKTLGVKAEYLAPETFDMAKMAQMIDAAAASKPDGLVVSIADPA
                     ALSEPVKNAVAAGIPVIVIDSGGGKLTKELGGLLYLGQSEYEAGVMAGERIKKLNVTK
                     AVCANHEVGNVSLDDRCAGFAKGLGVDVPVLNGVMDPTEMKNRIIAHLKTNADTQFIL
                     TVGIGGAEPGLMALDELGLTGKVKIGTFDLSPTILQATADGKMEWGIDAQQYAMGYIP
                     VVMFDLWKKYKIAPIADYPTGPGFVTGETAASVIELAKQGKR"
     gene            complement(8242..9141)
                     /gene="iolE"
                     /locus_tag="PKV23_00030"
     CDS             complement(8242..9141)
                     /gene="iolE"
                     /locus_tag="PKV23_00030"
                     /EC_number="4.2.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019860459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050114 - myo-inosose-2 dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inosose-2 dehydratase"
                     /protein_id="HQX83845.1"
                     /translation="MKVRIGINPITWSNDDAPELGGDIPLEVCLSETKQAGYAGTELG
                     GKFPRRTAELKPIMQRHGLAVISGWYDGRCDEKDVSAEMDAITPHLQLLKDMGSTHVV
                     YADTSRGRHGAIWDPISKRPALKAEEWPTYGRKLTQLAERMADFGVRMAFHHHMGTII
                     ETDAEVGLLMKHTGEAVGLLYDTGHSTFSGGDALQLIKDNVKRVVHVHCKDVRRPMLE
                     KARSQDMSFMAAVLDGIFTVPGDGFLDYASILRVLAEADYSGWLVVEAEQDPRKAHPL
                     TYATMGFKNLSRLAREAGFLVET"
     gene            complement(9138..10238)
                     /locus_tag="PKV23_00035"
     CDS             complement(9138..10238)
                     /locus_tag="PKV23_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010910921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HQX83846.1"
                     /translation="MQRLKVGVIGAGGIAQIEHIPNLMKLKRQFEILGVCDPSAKARD
                     FVSEEFGLATFESMAEVLALPLDAVVIASPDPLHHEQILAALERGFHVFCEKPLCYAP
                     GDIDALIAARDRARKVLQVGYMKRFDPSYEAAPGRMPGTAKTLRYVSVEVNDPDAWPF
                     IKHHAWRQGDDVVPELIASLKVKQQEQVARAVPVPLDDMSYRGFIAAYCSSLVHDVNA
                     VHGLLDGLGVPDGEIAGAQLFAGGEGGQGAVRLLGGRALWNMVHLKVPALADYSEKIT
                     FYFDEGILELEFPSPWLNHQPTRLTVKKSNGYTLVREDVRPGYGEAFIEELKGFWSAI
                     VEGKHVRNTAEQARRDMKLLCGLAQFYAGGHS"
     gene            10344..11795
                     /locus_tag="PKV23_00040"
     CDS             10344..11795
                     /locus_tag="PKV23_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010910920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HQX83847.1"
                     /translation="MTTAFPIDGIVIDRSIASNLHRQIYSQLRGLIERRVLPSGTALP
                     STRLLTKDLGVGRNTVIAAYEQLALEGYLLMRRGSPPLVMDLPSTVTATKEPHTEIGS
                     TLSARGKIMLAQPHHHGSPGLLAFHPGLPDSDNFPFTTWSKLLGKRAKLARHDLFGTY
                     HIGGYPPLCEAIARYLTASRGMTCTPEQIVITNGAQSAFDLLARILIDPGDTVWLEEP
                     GYYGAGSAFLSAGANLAPLHVSDHGWDLVPPPVPPRLIFVTPSCHQPLGVTMPMEQRL
                     NLIHMAERWNSWIIEDDYDSEYRFQGQPIPALHGISASNRVVYVGTFAKILFPAMRLG
                     FLVVPEAFREGLISALSVTGQFPPLLTQAALADFINEGHFARHLRRMRRLYAIRRQYF
                     METCEEYLSPWLRLRRTESGIQLVGLFHENHDDKAVAAAARDYGVNVSPLSIQYRHGE
                     VQGGLLMGFAAADEKTTKKAMGKLRDVLQATMG"
     gene            11835..12788
                     /gene="speB"
                     /locus_tag="PKV23_00045"
     CDS             11835..12788
                     /gene="speB"
                     /locus_tag="PKV23_00045"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013168927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HQX83848.1"
                     /translation="MITRKFNQPLGGNEMPRFGGPATMMRLPTQPTAKDLDACFVGIP
                     LDIGASNRAGTRFGPRQIRAESCLIRPYNMATRAAPYDSLMVADIGDVAIDTFNLPKC
                     MDIITNAYNTILSEGCKPLTLGGDHTIAYPILRAIAKKHGPVGLIHVDAHADVNEAMF
                     GEPIAHGTPFRRAVEEGLLDTKRCIQIGLRGTGYAADDFDWPRQQGFRVVQAEECWHK
                     SLTPLMEEVRKQMGSGPVYLSFDIDSLDPAFAPGTGTPEIGGLTTVQALEIIRGCWGL
                     NLVGCDLVEVSPPYDPSGNTALTAANLLFEMLCVLPGVKRR"
     gene            complement(12793..>13822)
                     /locus_tag="PKV23_00050"
     CDS             complement(12793..>13822)
                     /locus_tag="PKV23_00050"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HQX83849.1"
                     /translation="IMGETQRCGFITGGTWCVDNNRMVTHWPGEDGHAQFLREERHGG
                     GSGCNFALGVKQLDSSMPVETITIMGDDEGGRFLRALAADAGIGHAQMHVTSAVGTQW
                     TDAFVSQATGRRTHIFSRGASDVLTPDHFDFSSTTARYLHLGLPGVHKLMDAPWQDDA
                     NGWVSVLKKARATGLKTNLELASIDPQELAELVRPCLPHLDLIIVNDGEIGALAGQQT
                     LSGGVTDVEACKRAAREVLENGAMDLVVVHCPVIAIAVTRDGKVFTKPSTQIPRELVA
                     GANGAGDAFAAGMLYGLHEGWSIDDALALAHASAAASLRSLSTTGAVEPWRKCLALAN
                     VWGWRKAS"
BASE COUNT         2900 a         4193 c         3921 g         2808 t
ORIGIN      
        1 gtcatagcag ctttcgtcgc gcgtggcttc ggccacacgg taaccgatcg acagcaacag
       61 gcgccgtggt tcgagaagaa atccgaagtc cattcccagc gcgagttccc gcatttcagc
      121 aatgagaatc tccaatcgct ttttcattcc agcggccgcg tccggcccga ttgaagcatc
      181 tttgatgtgg ctttccaccg tctcacgcag agcggcggcc cacgaaagca gttgcctgcc
      241 tgcggtcgtg tcaagttccg ctgcataggt ggtggtggtc gcatgaatgt tcgcggcctg
      301 aaccgcaaaa tcgatcaggc ggaccagaat gtgctctggc gcttcacgcg ccttctcggt
      361 cgcccgcaaa agtgttgcca actgggattc aagttgcttg cggatgggtc gcaacatttg
      421 ccggtcgttc ggaatgtcgg aaaggtcttt ggccaggacg tcaagtgcgt cggcgatccc
      481 ctctaggctt gtctccacct tggcgggctc caccatccag gaggcgctgt aatttgccag
      541 ggcgatcagg tgtccggcga gattgccgct gtcgacggtg gaaacatacc gtggttcaag
      601 cggccggagg gttgttgttt cgtaccagtt gtaaaggtgg ccattgtatt tttccattcg
      661 cctcgctgtg gcaagggtgg cttcgattct gccgaccgca tcgctaagcc caagccagcc
      721 aaattcccgc gcactggcaa tggacaacag gtaaagcccg atgttggttg gtgatgtgcg
      781 gtgcgccacc agcggcaggg ggtcttcctg gaagttgtcg gggggaagca tgttctcagc
      841 gggcgtgaca aaggtttcga aaaaccgcca ggtccggcgc gccacgagcc gcagggtttt
      901 gcggtcttcc ggcgaagatt ccagttcatc ctccagcttc ggcgaacggc ttatccagaa
      961 tgccgcggcg ggtgcggcaa accacgcggc ggcaaacggc gccgctatga accagatgcc
     1021 gtcggcacga aacacagcaa tggccacagc cgcaactccg gccgccaagc tggccgacat
     1081 gacttggtaa ttcccagcca cgctgggtgt atggctggat tccgcttgcg ctgcggttgt
     1141 ccactccagc aggtttttgc ggcttgccgc aagcctgtac agggtccgcc caacagcatc
     1201 agccatgaga ccggcctgat ggccgagaaa aaccagattc gcgccggcca gaacaaaggc
     1261 atggaggaaa tcaccaaggg aagacctgat gcggctggtg acggtaactg gcagacgctt
     1321 ggggatggcc cctgaaagcg ctggaagcaa tggcggaaca acgcccgtga gaataataaa
     1381 tgccgtccat gttgcagcaa gtccggcagg aaggaatagc cagccaagca gaagcccgcc
     1441 gagcgtggca gtgggtgtca gcgaacgccg cagattgtcg gccattttcc agaggccaag
     1501 cggagggatt ttactattgg ggcgccgccc cagcatccac ggcagcaact gccagtctcc
     1561 gcgcgcccag cgatgctgcc gcgctgcggc aacagcgtaa cgctcgggat actcttcgac
     1621 gacttcaatg tcggtcacga gggcggcgcg ggcaaacacc ccttcgaaaa gatcatggct
     1681 taacatggtg ttttcgggaa tcttgcccgc tagcgccgct tcaaagatat caatgtcata
     1741 aatgcccttt ccggcaaatg acccttcccc aaacacgtcc tgatagacat cggaaacagc
     1801 aaatacatag ggatcaatgc cgcgggccga cgagaagatg cgttgaaata gcgacccgta
     1861 atggccaacc ggaagcgaag gggtgacgcg cggctgcaga acgctataac cttccacgac
     1921 acggccggct tgcgcgtcat attttgcttg attgagcgaa tgtgccagtt tcccaaccaa
     1981 gcgtcgtgcc gcgtcccgcg gcagctttgt gtcggaatcg agcgtgagga cgaaacggat
     2041 atcaggcggc agtttgccgc caatggcgca aaacgtcgtg tcggttgcgc cgcgcagcag
     2101 gcggttcagt tcatgaagct tgccgcgctt gcgttcccat cccatccatt tcccctcttg
     2161 cggattccac ttgcgtgaac ggtgcaacag gagaaagcgg tcggtgttat ggcggagatt
     2221 aagccgttgg attccttcaa gcgccacgtt gagaagcgtc gcatcggcag gaacatgctc
     2281 ggtatcggca tctgtccagt cggtcagcag ggcaaaatac aactctccat ccgcgttcga
     2341 caggtagtgg acttcaagcc ggtctaccag ctcctcgata ttgtctctcg aaatcaggag
     2401 ggtgggcacg acaaccaatg tccggaactg ggctggcaca ccttcacgca acgcaaggcc
     2461 tggaatgaca gcggcatcca tgaggcgggt gactgcgaaa ttgacaatcg caatgccgac
     2521 gtccgatgct gggaataggg cgagcagcat cagcactatc agcagtaagt aggaagtttc
     2581 accctgaaaa agcggggaca caccggcaag tagtgtaagc cccgtgagca gcgctacgct
     2641 gccgaggtat ccagccaggc cggtggcccg gaccgccatc ctcacacgcc gcaagagtgg
     2701 cggcttaaaa ccgagatcag gttcaaattt ggcgcggccc ggtccgatga gatgatatcc
     2761 gggatcagga gtgaccgctt cgtccgcgac acccgcgcct gtggccgtgt cgatggcgag
     2821 gcgcgcgatg gccagctcac tgtgattgga gccgcgtgac agctcttcaa ttgccgtgcg
     2881 ataaatcgtc cggctggcaa aatccatgtc gccgtaatgc gaatgggtgc gaagcaactg
     2941 gtcaacaagg ctgacgctgt cgaaccatac ttcccaattg acgtccgcaa tcaaccggag
     3001 gctggtaaca atattgcgca ccgtcacatt ggccgcagcc tggcgatggt gctcgtcgct
     3061 taccgcggct tcaaaagtgt agccgagctt gtctgtcttg tgtttgagcc agtcaagggc
     3121 ctggcctgcc acatcgccat ggtcgcgcag ccgctgaatc agttgcaccg cgaaggattt
     3181 ggagattgcg gtctcatcga tttcattgaa aagccgcgcc aggttggcgt gcttgtcgcc
     3241 aagatccagg acctggtcgg caagcttgtc tgccgtctcc cggccgcgcc gcgccgccat
     3301 gattctctgg gatatgcgcc ggaggttttc gatgaggatc agccgcaggc tgatcgccac
     3361 ggcccacagt tcgccgatgg tgagcggctg caccagctgg tactcgttga cgaaatccgt
     3421 gagtgttgcg ggattgaagt ggctgtcggt atgggcgaca taggcccaga ccagcccgaa
     3481 tattcgtgga tgtccggcca gcggcccttc ggcgagtttt ggcagctgcc ggtaaaatcc
     3541 cggcggaagg tccgcgcggg tctggaggat ctgttcttca acgaggtggt agttgtcaac
     3601 cagccattcg gccgctggtg ttatcgagtt gcctgccgcc agcgctgcgc agatttcgcg
     3661 gtaagctgcc agcaaaacag tcgcgttgtc gtccagccgg tcaatgatgg agtagactcg
     3721 gggaggatgg tcggtggtga tctgcgactc ggccaggctg cgcgcgtgtt gttttgatct
     3781 ctcgaggccg aagatttccg cactgattgg ctgggtatcg tcccacgcag agggagtatg
     3841 cctaagacgg tttccaatca tacgcagcat cgtgaactca tctccaaaaa catgtgaggc
     3901 cgtcagaaat gctgagagtc gcaagtttgc agaccagcaa tgcggggttg cccagctttt
     3961 taacgcgcga aatggcttaa ggttccagcc gcccgcgaaa aaacttcact gcggcgctgc
     4021 agggtttgtg tggatcacat tgaaagagct gaatattttt tgatcagccg ggaccactga
     4081 agggtctatt caatactgac ccaggccctg gccgctgaag attgcgcaaa agcgtgaatg
     4141 acgttttcga ttcgaagtcc gcgggcgaaa tcaatatggt ttgacggcgt ttgctggatg
     4201 gcatgaagga tttcagccaa ctcgatgact ttcagttcat tgaatccaag tccatgcccc
     4261 ggcgcggggc agaactttcc ataggccgga tgggcgggcg acgaaaggat gcggcgaaag
     4321 ccccgtgccg caaccggccc atccgccacg tagaggttca attcgttcat gcgctcgtta
     4381 tcgagccaaa gcatgccctt cgatccgtgc acctctattc tcaatccatt tttgcggcca
     4441 tgcgcaatcc ggcttgaact caagacgccc cgcgcaccgc tgcgaaagcg caccacggca
     4501 tgggcaatgt catcgttttc gacctgcgcc agcttgccgg cctcgccagc aagcggccgc
     4561 tggtcgtgaa ccacgtccgc catggcgcaa aggctgccga tttcacccac gagttcatgg
     4621 gccaaggaaa ccagatggca ggtcaaatcg ccaagcgtgc cgaggcccgc atcccggcgt
     4681 gtcaggcgcc atgaccatgg caacgacgga tcggccatat agtcttcgtc aacgctgcca
     4741 cggaaatcaa acacctctcc aatcgcccct tcggtgatca gctgcctggc atattgcagg
     4801 gcagggttgc gcaaatacgc ataacccaat gctgtgacct gtcctttggc cttcgccgct
     4861 gcagccgtca ttgcctccgc atcggcaagc gtgagcgcca ttggcttttc gcaccataca
     4921 tgcttgcccg cctcgagcgc ggcaacagcc atttcacggt gcatgccgtt gggcgatgtg
     4981 atcgaaataa gatggatggt cggatcggcc agcaggtcgc gccagtttgt agtggcgcta
     5041 ttaaagccga actccgaagc tttctgcctg gcgtgggaag aatccacgtc acagagcgcc
     5101 acccggtcaa tgtgcggaac gtcaccgaat accgacttaa cggcgccgaa ggccatggca
     5161 tggcatttgc ccatgaaacc ggttccgaca aggccaacac ctatgcggtt catgttcagc
     5221 gtttcccgtt gttgagtttg atgatttcct gttcgagatc gaccagttcc ttgccgcccg
     5281 ccatcagatt gagaagctgt tcgcggctga tctcgttctt ctggtaggtc cccgcactct
     5341 tgccccgctt gagaagcgtg aagcggtcac ccaccacata ggcatggtgc acattgtgcg
     5401 aaatgaagat gacgccaatg ccccgcgcct tggactgcac gataagcttc agcacaacgg
     5461 aggcctggtg aaccccgagc gccgatgtgg gttcgtcaag gatcaacacc ttggcgccga
     5521 aatggatcgc ccgcgcaatg gccaggcact gccgctcgcc gcctgaaagt gtccccaccg
     5581 gctgctcggg attgcgcaac tgaattccca tatccgaaag ctctttgtag gctgtgcgat
     5641 tggcatattc gatatcaaac tggcgaagga accaggtttt cttcaaaggc tcgcggccca
     5701 ggaaaaaatt gcgtgcaacg ctcatcagcg gcaccagcgc cagatcctga tacaccgtgg
     5761 caatgccctt atcgagcgtg tcctttggcg aactgaaacg tgtatgcgtg ccgcccacaa
     5821 gaatttcacc ttcatcgggg atatggaccc cggaaagtac tttgatgagg gtcgacttgc
     5881 ccgcaccatt gtcgccgaga aggcaatgca cttccccggg gcgcacatcg aaggatacat
     5941 ccttgagcgc gatgacggac ccgaaatatt tgctgacgga tttgagttcg acgagcggtg
     6001 tttccatcac cggctcctca tggcgcggct gcgcacatag ctgttgaaca gcaccgcaac
     6061 caccatcatg gcgcccatga aaagcttgaa ccagtccgtg tcgatgcccg tatagaatat
     6121 gcccatctgt accacgccga agataagcgc cccgaacatc gccccgattg ccgagccata
     6181 gccgccggtg agcaatgtgc ccccgatgac caccgcaatg atggcctcaa attccttgag
     6241 attgccgcgc agcgtgtcgg cggaaccggt tacgagcacc tgtatggtgg caaagagcgc
     6301 ggcggcagct gctgtcatca cgaaaagcgt gatcttggtg cgggccaccg gaacccccat
     6361 gttgcgcgcc gcactggctt cgccgcctga ggcaaatatc cagttgccaa accgcgtccg
     6421 catcagcagc catgtaaaga acagcgtcag tccaatccac cagatgattg ataccggaat
     6481 gccactgatc gcgggcaagc catcagcgcg tttgtcgatc atgcctaagt ccgcaagcca
     6541 gcggatgggt gtcctgaaaa cctggccggc aaagaggggg gtaatccagt ccccggcgga
     6601 atattcatgc aggcccgagg cctgggtgcg ccccgtgatg ccgcgcgtca ccccaagggt
     6661 gaggccgcgc agcatgaaca ggctgccaag cgtgacgatg aacgaaggaa gccccgtatg
     6721 caccacaatg aggccattca ggtagcccac gaaggcgcaa gctccgaaag ccatgagcac
     6781 ggaagcccat acgggccagt cccacatgat gggcggaatg gcgatgatga ttccggcgaa
     6841 gccgatcatt gagccaatcg aaaggtcgaa ctctcctgca atcatgagca gcgcaacggc
     6901 ggccgccaga ctgccgagct gagccgatac ctcgaggaag tttacgatgc ccttggcact
     6961 gaacaggccg ctgtcgcccg ccgttatgcc gaaaaacacg aagatcagaa ccgttccggc
     7021 aatggcccca agttcgggcc ggctgagcaa acggcctagt aacggcacag cacggacccg
     7081 ctcgtccttc acctcaaggc tcatcccatc ctccaagcaa tcattgccat ggcggcaggc
     7141 cattggccag ccgccatgaa aagatcagcg tttgccttgc tttgccagtt cgataaccga
     7201 ggcggccgtt tccccggtca cgaatcccgg gcccgtagga tagtcggcaa tcggcgcaat
     7261 cttgtacttc ttccacaggt caaacatgac caccggaata tagcccatcg catattgctg
     7321 cgcgtcgatt ccccattcca ttttgccatc ggctgtcgcc tgcagaatgg ttggtgaaag
     7381 atcgaaggtg ccgatcttga ctttgcccgt aaggccaagt tcgtcgagcg ccatcaggcc
     7441 cggttcggcc ccgccaatgc caaccgtcag gatgaactgc gtatccgcat tcgtcttgag
     7501 atgggcaatg atgcggttct tcatctccgt cgggtccatc acgccgttga gaaccggaac
     7561 gtcaacgccc agccccttgg caaagccggc acagcggtca tcgaggctga cgttgccaac
     7621 ctcgtggttg gcgcacaccg ccttggtgac gttgagcttc ttgatgcgct cgccggccat
     7681 gacgccagcc tcgtattcgc tttgcccgag atagaggagg ccgccaagct ccttggtcag
     7741 cttcccgccg ccggaatcaa tcacgatgac cggaatgccc gccgctaccg cgttcttcac
     7801 cggctcgctc agggccgccg gatcggcaat ggaaaccacg aggccgtcgg gcttcgaggc
     7861 cgctgccgcg tcaatcatct gggccatctt ggccatatcg aaggtttcag gcgccaggta
     7921 ttcggctttc acgcccagcg tcttggccgc atcgtccatg ccgtttttga caacggacca
     7981 atagggatcg ccgctttggc catgggtaac aaacaccacg cgggtgtctt cagcggcagc
     8041 aatacctgcc gccgcggata gcatcgtcag tccggccaga agaccgagca ttggtttcaa
     8101 tattttcaca agtgagaaca tgtttcctcc ttttaggttt ctccgccagc catgaacgcg
     8161 gctgacggac cagttgcatt ttcagtttct aggttacgct ttcgcactgg ttccttctgc
     8221 gggcggaatc ctccgcccga attatgtttc aaccaaaaat cccgcctccc tggccaggcg
     8281 cgacaggttc ttgaaaccca tcgtggcgta ggtcaggggg tgggcctttc ttggatcttg
     8341 ttcagcttcg acaacgagcc agcctgaata atctgcctcc gcgagaaccc gcaggattga
     8401 cgcatagtca aggaagccgt cgcccggcac ggtgaagatg ccgtcgagca ccgcggccat
     8461 gaaactcatg tcctggctgc gggctttctc aagcatcggt ctgcgcacat ccttgcagtg
     8521 cacatgcacc acgcgcttca cgttgtcttt gataagctgc aaggcatcgc cgccggagaa
     8581 agtcgaatgt cccgtgtcat agagaagacc aacggcctca cccgtgtgct tcatcagcag
     8641 tccaacttct gcgtctgttt cgatgatggt tcccatatgg tggtgaaagg ccatccgaac
     8701 cccgaaatcc gccatgcgtt cggcaagctg cgtaagtttg cgtccgtaag tcggccattc
     8761 ttctgctttt agcgcggggc gcttggagat cggatcccag atggcgccat ggcggccgcg
     8821 tgacgtatcc gcataaacaa catgggttga gcccatgtct ttcaggagct ggagatgggg
     8881 ggtgatcgca tccatttcgg cgctcacgtc tttttcgtcg catcggccgt cataccagcc
     8941 cgaaataacg gcaagcccat ggcgctgcat gatcggcttc agttccgctg tccggcgtgg
     9001 aaatttcccg ccaagttccg tcccggcata gccggcctgt ttcgtttcgc tcaggcaaac
     9061 ctcaagcggg atgtcgccgc caagttcggg ggcatcgtca ttgctccaag taatgggatt
     9121 gatgccaata cggactttca cgaatggcct cctgcgtaaa actgggcaag tccgcagagg
     9181 agcttcatgt cgcggcgcgc ctgttccgcc gtgttgcgaa cgtgcttgcc ctcaacgatg
     9241 gccgaccaga agcccttcag ttcctcaatg aaggcttccc catatccggg tctcacatct
     9301 tccctgacga gcgtatatcc attgctcttc ttgaccgtca gccgcgtcgg ctggtggttg
     9361 agccacggcg agggaaattc aagctcaaga atgccttcgt caaaatagaa ggtaattttc
     9421 tcgctgtagt ccgcaagggc agggaccttg aggtgcacca tgttccacag cgcccggcct
     9481 ccaagcagtc gcaccgcgcc ctggccgcct tcgccgccgg caaaaagctg ggccccggca
     9541 atctcgccat ccggaacgcc aagcccatca agaagcccgt gaaccgcgtt cacgtcatgg
     9601 acaagcgacg agcaatatgc ggcgatgaaa ccccggtaac tcatgtcgtc aagcggtacc
     9661 ggcacggcgc gcgccacctg ttcctgctgc ttgaccttca gcgaagcaat taactcgggc
     9721 accacgtcgt cgccctggcg ccaggcgtgg tgcttgatga agggccaggc atccggatcg
     9781 ttcacttcaa ccgacacata gcgcagggtt tttgccgttc cgggcatgcg cccaggggcc
     9841 gcttcgtagc tcgggtcgaa gcgtttcatg tagccgacct gcaggacctt tcgcgcccgg
     9901 tcgcgggccg cgatcaatgc atcaatatca cccggcgcat aacacagtgg cttctcgcag
     9961 aacacatgaa acccgcgttc cagggcggca aggatctgtt cgtggtgcag ggggtccggt
    10021 gaggcaatga caaccgcatc aaggggcagg gccagcactt cggccatgct ttcaaatgtc
    10081 gcgagcccga attcttcgct gacaaagtcg cgcgctttgg cggagggatc gcagacaccc
    10141 agaatttcaa actggcgctt tagcttcatc aggttcggaa tatgctcaat ctgggcaatt
    10201 ccgcctgcac cgatgacgcc aaccttcaaa cgctgcatcc ccacccacct cggacatgaa
    10261 acttgtctct ttatctggct tgagcctata accgtcttgg ttctaatgtt agaaccagaa
    10321 cgtgataaat ttcaggaacc accgtgacaa cagccttccc catagatggc attgttatcg
    10381 accgcagcat cgcctcaaat ctgcatcgcc aaatctatag ccagctccgg ggcttgatcg
    10441 aacgccgggt tctgccaagc ggaacggcac tgccttccac ccggctgctg acgaaagact
    10501 tgggcgtcgg ccgcaacacc gtcatcgccg cttacgaaca actggcactt gaaggctatt
    10561 tgctcatgcg gcgcggttca ccgcccctgg tcatggacct tccatcgacc gttaccgcaa
    10621 caaaagaacc gcacaccgaa atcggcagca ccttgtcagc ccgcggcaaa atcatgctgg
    10681 cacaacccca tcaccacggc agccccgggc ttctagcctt tcatcccggt ctgccagatt
    10741 ctgacaattt tccattcaca acctggtcaa agcttctcgg caagcgggcc aagctcgcgc
    10801 gccacgatct gtttggcact tatcacatcg gtggctatcc gcctctgtgc gaagcaatcg
    10861 cccgttatct tacggcctcg cgcggcatga cctgcacgcc cgaacagatt gtcatcacca
    10921 atggcgcgca atcagccttt gatctgttgg cacggattct catcgatcct ggtgacaccg
    10981 tgtggctgga agagccagga tattatggcg ctggttccgc cttcctgtcg gctggcgcca
    11041 acctcgctcc actccacgta agtgatcatg gttgggacct tgttccaccg cccgtgccgc
    11101 cgcgccttat ctttgtgacg ccctcctgcc accagccgtt aggtgtcacc atgcccatgg
    11161 aacagcgcct caatctcatc catatggcgg aacgctggaa ctcctggatc atcgaagacg
    11221 attatgacag cgagtatcgt ttccagggcc agccaattcc tgccctgcac ggcatcagcg
    11281 catcaaaccg cgtggtctat gtgggaacct ttgccaagat cctgttcccg gccatgcgcc
    11341 tgggttttct ggttgtgccg gaagcttttc gcgaaggcct tatctcggca ctcagtgtaa
    11401 ccggccagtt tccgccgctt ctcacccagg cggccctggc cgatttcatc aacgaaggcc
    11461 actttgcaag gcatttgcgc cgtatgcgca ggctttatgc aatacggcgc caatacttca
    11521 tggaaacttg cgaagaatac ctcagcccct ggctgcgcct gcgccgcacc gagtcaggaa
    11581 tacagctagt ggggctgttt catgaaaatc acgatgataa ggcggtggcg gcagcggccc
    11641 gggactatgg cgtaaatgta tcgccgctct ctatccagta tcgtcacggc gaggttcagg
    11701 ggggcctgct gatgggcttt gccgcagcag acgaaaaaac cacaaaaaaa gccatgggga
    11761 aactgcggga tgtgcttcag gcgacgatgg gataaggtca aggcattcat catcacgctc
    11821 aaggaaagac actcatgata acccgaaaat tcaatcagcc cttgggcggc aatgagatgc
    11881 cccgctttgg tggcccggcc acgatgatgc gcctgccgac ccaacccacg gccaaagacc
    11941 tggatgcatg cttcgtcggt atcccgctcg atatcggcgc ttcaaatcgc gccggcaccc
    12001 gctttggccc gcgccagatc agggccgaat cctgtttgat ccgtccctac aacatggcaa
    12061 cccgcgccgc cccctatgat agcctcatgg tggccgacat cggtgatgtg gcgattgata
    12121 cattcaatct gccgaaatgc atggacatta tcaccaacgc ttacaacaca atcttgagcg
    12181 aaggctgcaa gcccctgacg ctgggtggcg atcacaccat cgcctatcca atcctccgcg
    12241 ctatcgccaa aaagcacggt cccgtcggcc tcatccatgt cgatgcccat gcggacgtca
    12301 atgaggcaat gtttggcgaa cccatcgccc acggcacacc cttccgccgc gctgttgagg
    12361 aagggctgct cgataccaaa cgctgcatcc agattggcct gcgcggcacc ggctatgcgg
    12421 cggacgattt cgattggccg cgccagcagg gcttccgcgt cgtgcaggcc gaggaatgct
    12481 ggcacaaatc gctcactccg ctcatggaag aggtgcgcaa gcaaatgggc tccggccccg
    12541 tctatctgag ctttgacatt gatagcttgg accctgcctt cgcgccggga accggtacgc
    12601 cggaaatcgg cggcctcacc acggtccagg cgctggaaat catccgcggg tgctgggggc
    12661 tgaatctggt tggctgcgat ctcgttgaag tctcgccacc ctatgatccc tcgggcaaca
    12721 ccgcgctcac cgcagccaat ctcctgtttg aaatgctctg cgtcctgccc ggcgtgaaaa
    12781 gacgctgaaa actcaactgg ccttccgcca gccccataca ttggcaagcg caaggcattt
    12841 tcgccacggt tcgaccgcgc ccgtggtcga aaggctgcgc aatgaggccg ccgccgaggc
    12901 atgggcaagg gcgagcgcgt catcaatgct ccagccttcg tgaaggccgt agagcatgcc
    12961 cgccgcaaag gcatcacctg cgccgttggc gcccgcgaca agctcgcgcg gtatttgcgt
    13021 ggatggcttg gtgaaaacct tgccatcgcg ggtaaccgct atggcgataa ccgggcaatg
    13081 caccacgaca aggtccatgg cgccattttc aagcacctcc cgcgccgccc gcttgcaggc
    13141 ctccacatcg gtcaccccgc cggaaagggt ttgctggccc gcgagcgcgc cgatttcgcc
    13201 atcattcacg attatcagat caagatgggg aaggcagggg cgcacgagtt ccgccagttc
    13261 ctgcggatct atgctggcaa gttcaagatt ggtcttgagt cccgttgccc gggctttctt
    13321 gagaacgctc acccagccat ttgcatcgtc ctgccagggc gcatccatca gcttgtgaac
    13381 cccgggcagg ccgagatgca gatagcgcgc agttgtggaa ctgaagtcga aatgatccgg
    13441 cgtcagcaca tccgatgccc cgcggctgaa gatgtgggtg cgccgtccgg tggcctgtga
    13501 aacgaaggcg tcggtccact gggtgccaac tgccgaagtg acatgcatct gcgcgtggcc
    13561 gatgccggcg tcagcggcaa gcgcgcgcag gaaacgcccg ccctcgtcat cccccataat
    13621 ggtgatcgtt tccaccggca tgcttgagtc aagttgttta acgccaagcg caaaattgca
    13681 cccggatccg ccgccatggc gctcttcccg aaggaactgc gcatggccgt cttctcccgg
    13741 ccaatgcgtc accatgcggt tgttgtcaac gcaccatgtg ccgccagtga taaagccgca
    13801 gcgctgggtt tcccccatga tc
//
LOCUS       DAORVN010000002        10871 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_3106_length_10871_cov_3.365292, whole genome shotgun sequence.
ACCESSION   DAORVN010000002 DAORVN010000000
VERSION     DAORVN010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 10871)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10871)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10871
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3106_length_10871_cov_3.365292"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..845)
                     /locus_tag="PKV23_00055"
     CDS             complement(<1..845)
                     /locus_tag="PKV23_00055"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019860716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HQX83850.1"
                     /translation="MATVKSDIEIAREAKMKPIMEIGKALGIPEKDLIPYGHTKAKVH
                     LDYINSLSSKKDGKLILVTAISPTPAGEGKTTTTVGLGDGLNRIGKKAMVCLREPSLG
                     PCFGMKGGAAGGGYAQIVPMEDINLHFTGDFHAITSAHNLLSAMIDNHIYWGNELGID
                     TRRVAWRRVMDMNDRALREIVCSLGGVANGFPREAGFDITVASEIMAILCLSTSIEDL
                     ERRIGQIVIGAKRDKGQVLAKDVKAPGAMTVLLKDALMPNLVQTLENNPAFVHGGPFA
                     NIAHG"
     gene            1049..2626
                     /gene="ggt"
                     /locus_tag="PKV23_00060"
     CDS             1049..2626
                     /gene="ggt"
                     /locus_tag="PKV23_00060"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006206633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HQX83851.1"
                     /translation="MRNFDQPGRSLALGTNGMAATSSPLATLAAIDVLRQGGNAVDAA
                     VTASAILCLTEPHMTGIGGDCFVLIGRPNGTVLGLNGSGRSSARADAAWLKAARLTEI
                     APQSVHAVTVPGAIDAWDRLLQQEGTMTLGEALKPAIRLAEKGVPTTPRVARDWTESE
                     GLLAADEGGSQHYLKNGRSPRAGDIMAYPALAKTLKAIAKKGRAGFYEGEIVVEIVRH
                     LASRGGLLTAEDFAATEATWVKPIATRFAGHDILEIPPNGQGLTVLIALNILQHFGLK
                     RFEPESPERRHLEIEAVKLAWILRNRHIADPDFADIPVSDLLSDEYSQRLASLIDLNH
                     SSDGAVSLPASDTVYLTVADKNRLSVSFINSIYWDFGSGIVTPKSGITLQNRGRNFVT
                     QAGHPNCIGPAKRPLHTIIPAMVKKDGKIDMSFGVMGGDYQPMGHVTVLLNRYVYGMD
                     PQAALDFPRLYPKGEDVLTESTVPASVLAGLAAKGHRILPANEPLGGGQAIVIDHARG
                     LLTGASDHRKDGFALGY"
     gene            2733..4742
                     /locus_tag="PKV23_00065"
     CDS             2733..4742
                     /locus_tag="PKV23_00065"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HQX83852.1"
                     /translation="MSTDDPTATAFSFRTEEFTGPQAVSGASPAPRFTAVTDTSVLQN
                     IQRAVEAVGEATYHWTTETDAITWSPNMEAVIGCKPEAAASGRAYANLLDGDNITSRY
                     DTVMRSHAVDEGEGVAFQIEYLFRPDGRTGRRAVWLEDRGKWYVGKNGRPAEVFGTIR
                     RIDDRHSRDQHLSFLGNCDPLTGLMNRGRLAEALGETLSTANREQSNCAFLIAAVNNL
                     PVVNEAYGFEVADEVIVALSRRLRQVVRTGDAIARYSGSKFGIILNNCQEEELQTAID
                     RFINIARESVIETEHGPVWAMLSIGAVVVPKFAADANLAMARAEEALAEARRLPSDGS
                     AIYRPSAKRMAERGLNARCATEIVKCLKEDQFKLGYQPIVNAATGEVVMHEALLRMAD
                     ADGKLIPAGHLIPVAEKLGLVRLIDRAVAQMTISSLHNYPEARLSMNVSGSTATDPRW
                     FNQLTEIIAANQSIANRLTVEITETIALGDLKETFSFVEALRNIGCSVAIDDFGAGFT
                     SFRNLRDLPVNIVKLDGSFSRGLKENPANQYLVRTLVELTHKFELKAIAEWVETPEDA
                     EILRDCHIDFMQGKLFGDVAMTLPWASKEEQGFTSLDTPRQPAAVEQATPFTQSVAPV
                     EKPVAPESADMEEGLSRLKLAIKALDDQFRGSPAFSHDLPLAEAG"
     gene            4829..5584
                     /locus_tag="PKV23_00070"
     CDS             4829..5584
                     /locus_tag="PKV23_00070"
                     /inference="COORDINATES: protein motif:HMM:NF014068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HQX83853.1"
                     /translation="MNKPHDPTSAQSAAFARAQQFMSPSPVPSISTELLSTADGELNR
                     ELNHFLFDPPSNPSLLKGKKIAICCTNGVEEVEILGSMRWLSEHGATVHIVAPRVGEF
                     PPSLGLRFPEIARTHVLAIRLMENAGWLKIDCYLDEAKVADYDAVILPGGCWNPDFLR
                     ADKDAQNFTRDMHAAGKPTCGICHGPWVMVSAKMLKGKKATAVWNIQIDLENAGATVI
                     DEPCVVDGNLITARFPYDLPRMIDALAKQLVAA"
     gene            5999..6298
                     /locus_tag="PKV23_00075"
     CDS             5999..6298
                     /locus_tag="PKV23_00075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX83854.1"
                     /translation="MIRKTILTLAALLAISALGTSPARADIDIDINLGFGGGYLGKVS
                     CGTGARIVDRRFNRVVARDCRGSHYEYTGFRNGKWYRITLNSRTARIVAIRRWWR"
     gene            complement(6413..7018)
                     /gene="phaR"
                     /locus_tag="PKV23_00080"
     CDS             complement(6413..7018)
                     /gene="phaR"
                     /locus_tag="PKV23_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019965307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis repressor PhaR"
                     /protein_id="HQX83855.1"
                     /translation="MSDEKSAPKDPVVIKKYANRRLYNTATSTYVTLDDLSAMVKSGT
                     DFVVYDAKTGEEITRSVLTQIIFEEENKGTNLLPINFLRQLIRYYGDSMQTLVPGYLE
                     FSLENLTKEHDKIREQMLGAFTGEPFKNMEEHAKRNMAMFSDAMKLFNPFAAMAMNPG
                     AGTAPPKSNPSSAQAPSKDDLQAMKDQLTAMQEKINTLSKN"
     gene            7194..8381
                     /locus_tag="PKV23_00085"
     CDS             7194..8381
                     /locus_tag="PKV23_00085"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HQX83856.1"
                     /translation="MASPSSQDIVIVSAARTPVGSFNGALSSVAASFLGTVALKAAME
                     RAKVSPDEVNEVILGQVLTAAQGQNPARQAAIHAGIPNSATAWGLNQVCGSGLRAVAL
                     GMQQIQSGSATIVAAGGQESMSKSTHAAHLRDGVKMGDWSFIDTMIRDGLWDAFNGYH
                     MGNTAENVARQWQITRETQDAFAVASQNKAEAAQAAGKFKDEIAPVTIAGRKGDVIVS
                     DDEYIKKGVTLESVAKLKSAFMKDGTVTAANASGINDGAAALILMSAAEAAKRGLTPL
                     ARIASWATAGVDPAVMGSGPIPASQAALKRAGWKAADLDLVEANEAFAAQACAVNKDM
                     GWNPDIVNVNGGAIAIGHPIGASGARVLTTLLHEMQKRKAKKGLTTLCIGGGMGIAMC
                     VEN"
     gene            8391..9116
                     /gene="phbB"
                     /locus_tag="PKV23_00090"
     CDS             8391..9116
                     /gene="phbB"
                     /locus_tag="PKV23_00090"
                     /EC_number="1.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009210807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018454 - acetoacetyl-CoA reductase
                     activity [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetyl-CoA reductase"
                     /protein_id="HQX83857.1"
                     /translation="MGRVAVVTGGSRGIGAAISKALKAAGYKVAATYAGNDAAANAFK
                     SETGIPVYKWDVSNAEACAAGLKKVAEDLGPIEVLVNNAGITRDAMFHKMTAQQWNEV
                     IGTNLTALFNMTQPIWGGMRDRSFGRVICISSINGQKGQMGQANYAAAKAGEIGFVKS
                     LAQEGASKGITVNAICPGYIGTEMVRAVPQDVLEKRILPLIPVGRLGEPEEIARCVVF
                     LAADEAGFITGSTLSANGGQYMN"
     gene            9122..9721
                     /gene="ccmA"
                     /locus_tag="PKV23_00095"
     CDS             9122..9721
                     /gene="ccmA"
                     /locus_tag="PKV23_00095"
                     /EC_number="7.6.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011155771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015439 - ABC-type heme transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC exporter ATP-binding protein CcmA"
                     /protein_id="HQX83858.1"
                     /translation="MQLTASNLACERGGRTVFSNQNFSLNSGELLQLTGPNGSGKSSL
                     LRLLANLGEPSHGKISLEGGAPDLTPGQQAHYIAHSDASKSALTVTENLVFWRDFLGG
                     GDLMRALQAVNLAPLADYPAALLSAGQRRRLALARLALVPRPIWLLDEPGVGLDDAAQ
                     KLLAALMNGHLREGGLIVAATHVPLGLDPDKNLHLGAAP"
     gene            9718..10377
                     /gene="ccmB"
                     /locus_tag="PKV23_00100"
     CDS             9718..10377
                     /gene="ccmB"
                     /locus_tag="PKV23_00100"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HQX83859.1"
                     /translation="MKLLLALLRRDIILAARQGGGVGTAVGFFLTVLVLLPIGIGPDQ
                     ATLQRLAPGVLWITLLLSVLLSADRIFQQDFEDGSLEVMAMAPASLELVALTKAVAHW
                     LSTSLPLSIAAPFLGILLNLRTEIIWPLTLAMVLGSFALSLLAGIGASLTLNLRRGGL
                     LISILVLPLYVPILVFGMSATTGNPELIMPSLLILAAITLTSLVLAPIAAAAALRAYL
                     R"
     gene            10400..>10871
                     /gene="ccsA"
                     /locus_tag="PKV23_00105"
     CDS             10400..>10871
                     /gene="ccsA"
                     /locus_tag="PKV23_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018698781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HQX83860.1"
                     /translation="MQQFANPTRFLSLANRALPAIWTLTVILFAIGLYGALVSAPPDY
                     QQGETVRIMFVHVPAAWLAMLVYTVMALASAVAIIFRHPLADVAAKTAAPIGAVFCFL
                     ALATGSLWGKPMWGAWWVWDARLTSMFVLFLLYLGYIAIWQAIEEPTRAAMIARI"
BASE COUNT         2106 a         3583 c         3029 g         2153 t
ORIGIN      
        1 cagccatggg cgatgttggc aaacggcccg ccatgcacga aggccggatt gttttccagc
       61 gtctgcacca ggttcggcat cagcgcatcc ttgagaagaa ccgtcatggc gcccggcgcc
      121 ttgacgtcct tggcaagaac ctggcccttg tcgcgcttgg cgccgatcac gatctgcccg
      181 atgcgccgtt ccaggtcttc aatggaggtg gaaaggcaga ggatcgccat gatctcggag
      241 gcaaccgtga tgtcaaagcc cgcttcgcgc gggaatccgt tggccacgcc gcccagcgag
      301 cagacgattt cacgcaaggc ccggtcgttc atgtccatca cccgccgcca ggccacgcgc
      361 cgtgtatcga tgccaagctc gttgccccaa tagatgtggt tgtcgatcat tgccgacaaa
      421 agattgtggg ccgaggtgat ggcgtggaag tcgccggtga aatgcaggtt gatgtcttcc
      481 atcggaacga tttgcgcata accgccgccc gcagcacccc ccttcatgcc gaaacagggg
      541 cccagcgaag gctcgcgcag gcacaccatc gccttcttgc cgatgcggtt gaggccgtcg
      601 cccaggccca ccgttgttgt cgtcttgcct tcgccggcag gcgtcgggct gatcgccgtc
      661 accagaatga gcttcccgtc cttcttggaa gaaagactgt tgatatagtc aagatgcacc
      721 ttcgccttgg tatggccata ggggatgaga tccttctccg ggatgcccag ggccttcccg
      781 atctccatga tcggcttcat ttttgcttcg cgggcgatct cgatgtcgct ctttaccgtg
      841 gccatggtcg ggtcatcctt ccttaagaga aaatttcatt catgccggga aacagcacat
      901 ttcgttcaaa aagtccatcc gttggcagga gtgccaaact gcggcaagag cagcttgcgg
      961 gctttcttgc aatatagatc accacaatgc gatgctgctt gcgacagttt tgcggcagtt
     1021 tttgccgcca gtgaaaaagg atatagttat gagaaatttc gaccagccgg gacgttcgct
     1081 ggccctcggc accaacggca tggccgccac ctcgtcgccg ctggccaccc ttgccgccat
     1141 tgatgtgctg cgccagggcg gcaatgcggt tgatgcggcc gtcaccgcct cggccattct
     1201 ctgcctcacc gagcctcata tgaccggcat cggcggcgac tgcttcgtcc tcatcggcag
     1261 gcccaatgga accgtgctgg gcctcaatgg ctcggggcgc tccagcgcca gggctgatgc
     1321 cgcttggctg aaagccgcaa ggctgacgga aattgccccg caaagcgtgc acgccgtcac
     1381 cgtgcccggc gccatcgacg cctgggaccg gctcctgcaa caggaaggca ccatgacgct
     1441 gggtgaagcc ctgaagcccg ccatcaggct ggcggagaaa ggcgtgccga ccacgccgcg
     1501 cgttgcccgg gactggacgg aaagcgaagg cctgcttgcc gccgatgaag gtggctccca
     1561 acattatctg aaaaacggcc gcagccctcg cgcgggcgac atcatggcct atcccgccct
     1621 ggccaagacc ctgaaggcaa ttgcgaagaa gggccgcgcc ggattctatg aaggcgaaat
     1681 cgtcgttgag atcgtcaggc atctggcgtc ccgcggcggg ttgctcaccg ccgaagattt
     1741 tgccgcaact gaagcaacct gggtgaagcc catcgccacc cgctttgccg gccacgacat
     1801 tctggaaatt ccgcccaacg gccagggcct cacggtcctc atcgccctca acatcctgca
     1861 gcatttcggc ctcaagcgtt ttgagccgga aagccccgag cgccgccatc ttgaaattga
     1921 agccgtgaaa cttgcctgga tcctgcgcaa tcgccacatc gccgaccccg attttgcgga
     1981 cattccagtg agcgatcttc tgagtgatga gtactcgcaa agactggcca gcctcattga
     2041 cctcaaccac agcagtgatg gcgccgtctc gctccccgct tccgatacgg tttacctgac
     2101 ggttgccgac aagaaccgcc tgagcgtttc cttcatcaat tccatctatt gggattttgg
     2161 ctccggcatc gtaacgccga aatccggcat cacgctgcag aaccgtggaa ggaatttcgt
     2221 cacgcaggct ggacatccca attgcatcgg ccctgcaaag cgccccctgc acaccatcat
     2281 tcccgccatg gtcaagaagg atggcaaaat cgacatgagc tttggcgtca tgggtggcga
     2341 ttaccagccc atgggccacg tcactgtcct gctgaaccgc tatgtctacg gcatggaccc
     2401 gcaagcggcc ctcgattttc cccggctcta tcccaagggc gaagacgtct tgacggaaag
     2461 cacggttccc gcctccgtcc tggccggtct cgccgcgaaa ggccatcgca tcctgccggc
     2521 gaatgagccc ctgggaggag gacaggctat tgtcatcgac cacgccagag gcctgctgac
     2581 cggcgcctct gaccatcgca aggatggttt tgcccttggc tactagccgt ccccaatcct
     2641 taccggcaaa ttaatcacag attttttgta tctgtttacg attgctcggc tagtttgcca
     2701 gggtgaattt tgcgtaagct cttggaggcg acttgagcac cgacgaccct accgcaacgg
     2761 cgttttcctt ccggactgaa gagtttaccg gtccgcaagc cgtatcgggc gcttcgccag
     2821 cgccgcgttt tacggcggtg accgacacca gcgtcctgca gaacatccag cgtgccgtcg
     2881 aagccgtggg cgaagccacc taccattgga ccaccgaaac cgacgccatc acctggagcc
     2941 ccaacatgga ggctgtcatc ggctgcaagc ccgaggcggc ggcctccggc cgggcctatg
     3001 ccaatctcct cgatggcgac aacatcacca gccgctacga tacggtcatg cgcagccatg
     3061 ccgtcgatga gggtgaaggc gttgcctttc agatcgagta tcttttccgc cccgacggcc
     3121 gcaccggccg ccgcgccgtt tggctcgagg accgcggcaa gtggtatgtc ggaaagaacg
     3181 gcaggccggc cgaagtcttc ggcaccatcc gccgcataga tgaccgccac agccgcgatc
     3241 agcacctgag ctttctcggc aattgcgatc ccttgaccgg cctgatgaac cgcggccgtc
     3301 tcgccgaagc gcttggcgaa accctctcca ccgccaaccg cgaacagagc aactgcgcct
     3361 tcctcatcgc cgccgtgaac aacctgcccg tcgtcaacga agcctatggc ttcgaggtgg
     3421 ccgatgaggt gatcgtggcg ctgagccgcc gcttgcgcca ggtggtgcgc accggcgatg
     3481 ccatcgcccg ctattccggc agcaagttcg gcatcatcct gaacaattgc caggaagaag
     3541 agttgcagac cgccattgac cgcttcatca acatcgcccg tgaaagtgtc atcgaaaccg
     3601 agcacggccc cgtctgggcc atgctttcca ttggcgccgt cgtcgtcccc aaatttgccg
     3661 ctgatgcgaa cctcgccatg gctcgcgccg aagaggcgct ggcagaagca aggcgcctgc
     3721 cgtccgatgg ctcggccatt taccggcctt cggccaagcg catggccgaa cggggcctga
     3781 atgcaaggtg cgccactgaa atcgtgaaat gcctgaagga agaccagttc aagctcggct
     3841 accagcccat cgtcaatgcg gcaaccggcg aggtggtcat gcacgaggca ctgctgcgca
     3901 tggccgatgc tgacggaaag ctcattcccg cgggccacct cattccggtc gccgaaaaac
     3961 tcggactcgt gcgcctcatc gaccgcgccg tggcccagat gaccatttca tccctgcaca
     4021 actatcccga ggcccggctt tccatgaatg tctcgggctc gaccgccacc gatccgcgct
     4081 ggttcaacca gctcaccgag atcattgctg ccaaccagtc aatcgccaac cggctcaccg
     4141 tggaaatcac cgaaaccatt gccctgggcg atctcaagga aaccttcagc ttcgtcgagg
     4201 cgctgcgcaa catcggctgc agtgtcgcca tcgatgattt cggcgccggc ttcacttcat
     4261 tccgcaacct gcgcgacctg ccggtcaaca tcgtaaaact cgacggctcc ttcagccgcg
     4321 gcctcaagga aaatcccgcc aaccagtatc tggtgcggac actcgtcgag ctcacccaca
     4381 agttcgagct gaaagccatc gccgaatggg tggaaacccc ggaagacgcc gaaatcctga
     4441 gggattgcca tatcgatttc atgcagggca agctcttcgg cgatgtcgcc atgacgctcc
     4501 cctgggccag caaggaagaa cagggcttca ccagcctgga cacgccgcgc cagcccgctg
     4561 ctgtggagca ggccacgcct ttcacccaga gcgttgcgcc tgttgaaaag ccggttgcac
     4621 cagaaagcgc cgacatggaa gagggcctgt cgcgcctgaa gctggccatc aaggccctcg
     4681 atgaccagtt ccgcggttcc ccggccttca gccacgacct gcccctcgcc gaagccggtt
     4741 aagttccggc cagtctgcat ctttctctgg acgatcccgc tgtttcctga gaaaactcct
     4801 gctttcctat gcaaccagga gtgccgccat gaacaagccc catgatccca catcagccca
     4861 gagtgccgcc tttgcccggg cccagcaatt catgtcgccc tcgccggtac cctccatttc
     4921 caccgagctt ctctccaccg ccgatggcga actgaaccgc gaactcaacc atttcctctt
     4981 cgacccgccg tcaaatccca gcctgctcaa gggcaagaag atcgccatct gctgcaccaa
     5041 tggcgtcgag gaagtggaga tcctcggctc catgcgctgg ctttccgagc atggcgcaac
     5101 cgtccatatc gtggccccgc gcgtcggcga atttccgcca agccttggcc tgcgctttcc
     5161 ggaaattgcc aggacccatg tgctggccat ccgcctgatg gagaatgccg gctggctgaa
     5221 gatcgactgc tatctcgacg aggcgaaggt ggccgactat gacgcggtga tccttcccgg
     5281 cggctgctgg aatccggatt tcctccgcgc cgacaaggac gcgcagaatt tcacccgcga
     5341 catgcatgcg gccggcaagc ccacctgcgg aatctgccac ggcccctggg tcatggtcag
     5401 cgccaagatg ctgaaaggca agaaggccac cgccgtgtgg aatatccaga tcgacctgga
     5461 aaacgccggc gccacggtga tcgacgaacc ctgcgttgtc gacggcaacc tcatcaccgc
     5521 caggttcccc tacgacctgc cccgcatgat cgacgcgctg gcgaagcaac tggtggctgc
     5581 ctagctgttc cgcgagacag gaaaaatccg gcactgaatt ccgggccggt ggaatattta
     5641 atccctttat aggatttaaa gcttgatttt gaatccctgt cgtgctagtc atggtccgtt
     5701 catcccggag agtcgctgat catgacagtg atctcgaagg ggtggatggg gcctccgggc
     5761 ttggggcgta acgttccctc aactccggaa gcttcaggcg ggatttcccg ccgccgctgt
     5821 ttgacattgt gtaacctacc ccggaagcgg agcgccgctt tcttcccgga aggcctgtgg
     5881 ccggcgccag gaaaaattga atcaaaatgg aattctcaca ggcattctta tttgcgtggg
     5941 ccagcgcctt gaaataaagt ttgggttgca aaacaaatac gcaaccaggg agcctgtcat
     6001 gatccgcaaa accatcctca ccctagcagc gctgctcgca atttctgcgc tgggcacaag
     6061 ccccgccagg gccgatatcg acattgatat caatcttggt tttggcggcg gttatcttgg
     6121 aaaggtctcc tgcggcacgg gcgccaggat tgtggaccgg cgcttcaacc gggttgtcgc
     6181 ccgtgattgc cggggcagcc attatgagta cacgggcttc cgcaatggca aatggtatcg
     6241 catcaccctg aattcgcgga ctgcgcgcat cgtggccatt cgccgctggt ggcgctaggg
     6301 atcggtccca tccgccaaat ctcatcatgg ccgtgctcga cacggccatc ttctttgttg
     6361 ccggaaagga gatgcccgca tcaagtgcgg gcatgatggg aaggaaaaac cgctaattct
     6421 tcgacagcgt gttgattttt tcctgcatcg ccgtgagctg gtccttcatc gcctggagat
     6481 cgtccttgga cggggcctgc gcggacgatg gatttgattt tggcggcgct gtcccggcac
     6541 ccggattcat cgccatcgcc gcgaacggat tgaacagctt catcgcgtcg ctgaacatcg
     6601 ccatgttgcg cttggcatgc tcttccatgt tcttgaacgg ctcgccggta aaggccccca
     6661 gcatctgctc gcggatcttg tcgtgctcct tggtgagatt ctccagcgag aattccaggt
     6721 agcccggaac cagcgtctgc atgctgtcgc cgtaatagcg gatgagctgg cgcaggaaat
     6781 tgatgggcag caggttggtg cccttgttct cttcctcgaa gatgatctgg gtcagcaccg
     6841 agcgggtgat ttcctcgccg gtcttggcgt catagaccac gaaatccgtg cccgacttga
     6901 ccatggcgga aaggtcatca agggtcacat aggtgctcgt tgcggtattg tagagcctgc
     6961 ggttcgcgta tttcttgatc accaccggat cttttggcgc cgatttttcg tcactcaccg
     7021 tttacactcc tccagtacgg accatcaccg attgtctccc cgatgccttg cccgcagtgc
     7081 aaaaacttgc tccccgcaat gcacaattgc aagtgcaaaa aactatcgcc tcatgtgttt
     7141 caaatgctat gactaacgga acattccctt ttcgagcctc caggagtaag ccagtggcat
     7201 caccatcttc ccaagacatc gtcatcgttt ccgccgcccg taccccggtt ggctccttca
     7261 atggagcctt gagctcggtt gccgcctctt tcctcggcac cgtggccctg aaggccgcca
     7321 tggagcgcgc caaggtttcg cccgatgagg tcaacgaggt cattctgggt caggtcctga
     7381 ccgccgccca gggccagaac cccgccaggc aagctgccat ccacgccggc attccaaact
     7441 ccgccaccgc ctggggcctg aatcaggtct gcggctcggg gcttcgcgcc gtggcgctgg
     7501 gcatgcagca gatccagtcg ggaagtgcca ccattgtggc cgctggcggc caggaatcaa
     7561 tgagcaagtc aacccacgcg gcgcacctgc gcgacggcgt gaaaatgggt gactggagct
     7621 tcatcgacac catgatcagg gatggcctgt gggacgcctt caacggctat cacatgggca
     7681 acaccgccga gaatgttgcc aggcaatggc agatcacccg cgaaacccag gatgcctttg
     7741 ccgtggcttc gcagaacaag gccgaagcgg cccaggccgc cggcaagttc aaggatgaaa
     7801 ttgcccccgt caccattgcc ggccgcaaag gcgacgtgat tgtctccgac gatgaataca
     7861 tcaagaaggg cgtgaccctt gaaagcgtcg ccaagctgaa atccgctttc atgaaggatg
     7921 gcaccgtgac cgccgccaat gcctccggca tcaatgacgg cgccgccgct ctaattctga
     7981 tgagtgctgc tgaagctgca aagcgcgggc tcacgcccct cgcccgaatc gcctcctggg
     8041 ccacggcagg tgttgacccc gccgtcatgg gctccggccc cattcctgcc tcgcaggccg
     8101 cgctgaaaag agccggctgg aaagccgccg accttgatct tgtggaagcc aatgaagcct
     8161 tcgccgccca ggcctgcgcc gtgaacaagg acatgggctg gaacccggac atcgtgaatg
     8221 tgaacggcgg cgccattgcc atcggccacc ccatcggcgc ttcaggagcc cgcgtgctca
     8281 ccaccctgct ccatgaaatg caaaagcgta aagccaaaaa gggcctgacc accctgtgca
     8341 tcggcggcgg catgggcatt gccatgtgcg tagaaaacta agggggcata atgggaagag
     8401 ttgcagtcgt caccggtggc agccgcggca taggcgccgc catttcaaaa gctttgaagg
     8461 cggcgggcta caaggttgcc gccacttacg ccggcaatga cgcggccgcc aatgccttca
     8521 agagcgaaac cggaattccg gtttacaaat gggatgtctc caatgccgag gcctgcgccg
     8581 ccggcctgaa gaaagtggcg gaagacctgg gacccatcga ggtgctggtg aacaatgccg
     8641 gcattacccg tgacgccatg ttccacaaga tgacggccca gcaatggaat gaggtcatcg
     8701 gcaccaatct caccgccctg ttcaacatga cccagcccat ctggggcggc atgcgcgacc
     8761 gcagtttcgg ccgggtcatc tgcatctctt ccatcaacgg ccagaagggc cagatgggcc
     8821 aggccaacta cgccgcagca aaagcgggcg agatcggctt cgtgaaatcc ctggcccagg
     8881 aaggggcgtc caaaggcatc accgtcaacg ccatctgccc cggctatatc ggcaccgaaa
     8941 tggtgcgcgc cgtcccccag gacgtcctgg aaaaacgcat tctgccgctg atccccgtcg
     9001 gccgccttgg cgaaccggaa gaaattgccc gctgcgttgt ctttcttgcc gccgatgaag
     9061 cgggcttcat caccggctca accctgtccg ccaacggcgg ccagtacatg aactgatatt
     9121 gatgcagctg accgcctcga acctggcttg cgagcgcggc ggcaggacgg ttttcagcaa
     9181 ccagaatttt tccctcaata gcggcgaact cctgcaactc accggaccca atggttccgg
     9241 caagtcatcg ctgctccgcc tgcttgcaaa cctcggcgaa cccagccacg gcaaaatttc
     9301 tctcgaaggc ggcgcgccag atctcactcc gggccagcag gcacattaca tagcccacag
     9361 cgatgccagc aaatccgctc tcactgtcac ggagaatctg gttttctggc gcgacttcct
     9421 cggcggcggc gatctcatgc gcgccctgca ggctgtcaat ctcgcgccac ttgcagacta
     9481 tcccgcggca cttctctccg ccggccagag gcgccgcctg gcacttgcaa gactggcgct
     9541 tgtcccccgt ccaatctggc tgctcgatga gcccggcgtg ggattggatg acgccgccca
     9601 gaaactgctc gccgccctca tgaacggcca tctccgggaa ggcgggctca ttgtcgccgc
     9661 aacccatgtc cctcttgggc ttgaccccga caagaatctc cacctcgggg cagcgccatg
     9721 aaactgctgc tcgcccttct ccgccgcgat atcatcctcg ctgcccgcca gggcggcggt
     9781 gtcggaaccg ccgttggatt cttcctgacc gtgcttgtgc tgctgcccat cggcatcggc
     9841 cccgaccagg ccaccctgca acgccttgcc cccggcgttc tctggatcac cctgctgctc
     9901 tcggtgctgc tttccgccga ccgcattttc cagcaggatt tcgaggacgg ctcgctggag
     9961 gtcatggcaa tggcgcccgc ctcccttgaa ctcgtggccc tcaccaaggc cgtggcccac
    10021 tggctgtcca ccagcctgcc gctcagcatc gcagcgccct ttctcggcat tctgctcaac
    10081 ctgagaaccg agattatctg gccgctgacg ctggccatgg ttctgggctc attcgccctc
    10141 tcgcttctgg ccggcatcgg cgcctccctc accctcaatc tccgccgtgg cggcctgctc
    10201 atttcgatct tggttctgcc gctttatgtg cccatcctcg tcttcggaat gtcagccaca
    10261 accggaaatc ccgaattgat catgccatcc cttctcattc tggccgccat caccttgacg
    10321 agcctcgtgc tcgcccccat cgccgctgct gcggcgctac gcgcctatct ccgatgatcc
    10381 aatcttcgta gaataagaca tgcagcaatt cgccaacccg acccgctttt taagtctagc
    10441 caatagggcg ctgccagcga tctggacgct gaccgtcatc ctctttgcca tcggcctcta
    10501 tggcgcgctt gtctcagcac cccccgatta ccagcagggc gaaaccgtgc gcatcatgtt
    10561 cgtccacgtg cctgccgcct ggcttgcgat gcttgtctat acggtcatgg cgctggccag
    10621 cgccgtggcc atcatcttcc gccatcccct cgctgatgtg gccgcaaaga ccgccgcccc
    10681 catcggcgcc gtcttctgct ttctcgccct cgccaccggc tccctctggg gcaagcccat
    10741 gtggggcgcc tggtgggtgt gggacgcgcg gctcacctcc atgttcgtgc tcttccttct
    10801 ttatctgggt tacatcgcca tctggcaggc catcgaggag ccgacccgtg ccgcgatgat
    10861 cgcccgcatc g
//
LOCUS       DAORVN010000003        10370 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_3349_length_10370_cov_3.091614, whole genome shotgun sequence.
ACCESSION   DAORVN010000003 DAORVN010000000
VERSION     DAORVN010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 10370)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10370)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10370
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3349_length_10370_cov_3.091614"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="PKV23_00110"
     CDS             complement(<1..201)
                     /locus_tag="PKV23_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007794642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HQX83861.1"
                     /translation="MFWKLTLAIALGFILGAVALGFLPLLWLGFGQAFHIDDYLPRVV
                     VFTLKQAFLSTLLSVAIALPVAL"
     gene            complement(201..1196)
                     /gene="thiB"
                     /locus_tag="PKV23_00115"
     CDS             complement(201..1196)
                     /gene="thiB"
                     /locus_tag="PKV23_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018328605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA];
                     GO_process: GO:0030974 - thiamine pyrophosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HQX83862.1"
                     /translation="MLKIFSTIIASVIFASLAQAKETLTIYTYESFVTEWGPGGKVKE
                     AFEKTCDCSIEWVGLGDGVAVLNRLKLEGANTKADLVLGLDTSLTAEAIGTGLFGKHG
                     VDKGLAKTPEAWADDYFMPYDYAHFAVIYDTEKMASPPKSLDELVNGDPAQKIVLEDP
                     RSSTPGLGFLLWMKAVYGEGAAEQWKKLQPRILTITPGWSEAYGLFTKGEAPMVLSYT
                     TSPAYHMVAENTPRYQAAAFSEGHYLQIEVAGLIEASPEKALAQKFLGFMMTPAFQDI
                     IPTTNWMLPAGATDTALPEAFGKLVAPGKTLLFSPDEVFKNRRDWVDEWLNALSK"
     regulatory      complement(1243..1401)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            1459..2094
                     /locus_tag="PKV23_00120"
     CDS             1459..2094
                     /locus_tag="PKV23_00120"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008128301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HQX83863.1"
                     /translation="MSRFAILLGGDLVVTPRLRRQIAGARIIAADSGMKHASALGLMP
                     ELWVGDFDSAGSELLLDYALVPRQTHSADKDATDGEIAVAEALRLGATEIVLIGGLGG
                     ESDHATAHLGLALHLARSGQKCFISNGDEEAYPLTAGTYEFDFGPQSRFSIVPWSDIT
                     GLHLTGVKWPLKDKALTLGTSFTMSNVALGPVKLSFSSGIAIAIAYPTQTD"
     gene            2096..3271
                     /locus_tag="PKV23_00125"
     CDS             2096..3271
                     /locus_tag="PKV23_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011504687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HQX83864.1"
                     /translation="MQVTIEYLNWRGEGVGGGQTFRNVLGGETVDAATGQVLQSSPER
                     IDPFCKHYVKCGGCQLQHWQQLPYEAWKRNLITTALEKRGINFQVGDIIDAHGTGRRR
                     VSLHVRKRNGIVQAGFMAARSHDLIDIDQCPILVPALQNSTDIARKLGAKLGDCDVSI
                     KAADNGLDVSIKAERKIADEQVPKLATLVEELNLARLSINGNLVASRAVPVVSIGKAL
                     VQVPPGSFLQATKAGEEALANLVLASLGKSKSVADLFCGVGPFALRIAERAKVTAIDS
                     DKPAITSLAQASRLTTGLRPLKAEVRDLMREPLVASELKEFDTVVFDPPRAGAEAQAR
                     QLAKSTVKTIVAVSCDPATFARDAEILVGGGYKQKSLTAVDQFKWTSHVEIVGTFGR"
     gene            complement(3268..3840)
                     /gene="efp"
                     /locus_tag="PKV23_00130"
     CDS             complement(3268..3840)
                     /gene="efp"
                     /locus_tag="PKV23_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012114551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HQX83865.1"
                     /translation="MVKVIASGVRKGNVIEHEDGKLYVVLKSESMHPGKGTPTTTIDM
                     RRISDGVKTVVRYKTTDAMERAFVETVTHSYLYKDGDMYVFMNPESFEQISLHEDMVG
                     DAAPFLQEGMECQIALFNGAPISIELPQRVTLEIMETEPAIKNQTASSSFKPALLSNG
                     VRTLVPPHIVSGTRIVVETADGSYVERAKD"
     gene            3933..4949
                     /gene="epmA"
                     /locus_tag="PKV23_00135"
     CDS             3933..4949
                     /gene="epmA"
                     /locus_tag="PKV23_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012455062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P lysine aminoacylase EpmA"
                     /protein_id="HQX83866.1"
                     /translation="MPWFSPERHKDRRPFLLARNRILAGFRQWFDERGFLEVDCAALQ
                     VSPGNEAHLHAFATDLIQPDGSRERRYLHTSPEFACKKLLAAGETRIFSLAHVFRNRE
                     RTLTHSPEFTMLEWYRANEPYAAIIDDCLALVRLAAIISESKQFNWRHRKADPFAEAE
                     WLSVAWAFEKYAGVDLLATLNGSESNREALAKQCPVQFTDRDSWSDIFSRILVEKVEP
                     NLGNGTVTVLYEYPACEAALARVCTHDPRVSERFELYACGVELANGFGELTDPVEQRR
                     RFETEMNLKEQLYGERYPVDEELLQALRLMPPASGVALGLDRLIMLATGATRIGQVQW
                     TPVS"
     gene            5225..6844
                     /locus_tag="PKV23_00140"
     CDS             5225..6844
                     /locus_tag="PKV23_00140"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015668747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HQX83867.1"
                     /translation="MAGTAPLDFGLYDYIIIGAGSAGCVLANRLSASGRHRVLLLEAG
                     GEDRNPWIHIPIGYAKLFNNHHYNWMYETEPEPELDGRKIYQPRGKVLGGTSSINGLV
                     YIRGQKEDFDQWARMGNTGWSFADVLPYFKRSEDQENGADAYHGAGGPLAVSNPREPH
                     ELCEAFIRAGMERGLPRREDFNGADQEGIGYFQTTSRNGRRCSTAVGYLRPARGRKNL
                     HIVTHALAHRIVFEGRRAAGVEFLHQGRIKLARAGREVVLAAGAINSPQLLELSGCGQ
                     AERLNGFGIPVILDAPQVGENLQDHLQVRMVFRCKKPITLNDQYRNLFRRVGIGLRYA
                     VQRKGPLAVSAGYGTAFFRSDDRIATPDIQIHFIIFSTDRMGQNLHPFPGFTASVCHL
                     RPESRGSIHLRSADPGQSPSIRVNYLSTDEDRRAVVDGLKFLRSIMQAPAMLDYASEE
                     IEPGPACASNQDLLAYCRAKGSTIYHPSGTCRMGVDPGAVVSPDLKVKGLSGLRVADG
                     SIMPQLVSGNTNAAIIMIGEKASDLILGESL"
     gene            complement(6989..8323)
                     /locus_tag="PKV23_00145"
     CDS             complement(6989..8323)
                     /locus_tag="PKV23_00145"
                     /inference="COORDINATES: protein motif:HMM:NF005685.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HQX83868.1"
                     /translation="MTAAPAIPPNIHSTGSLPKVVWAKGSYLHDVDGKQYIDGSGGPA
                     VYCIGHSNAEVNEAIKSQLDRIAHGYRYNFTTDALEELTEIIRGVCGGTLKHVVYVTG
                     GSEAVESCLKLALQYHAARGEMSRRRFIARERSWHGNTLGALSVSGFLERKAAFEGSL
                     LDVSRLSPANAYRPIAANVGEACAQELEDEILAVGPDKIAAFIFEPVVGAAGGCVPAP
                     AGYARRVREICDKYGVLMIADEVMCGSGRCGTWRALETDGVEPDIMSVAKGLAAGYLP
                     LGAAIYTDRVAEPIMRVHGAPMTGHTFTGHTACCAAAVAVQKIVAREGLVARVRANEP
                     KLKKMIAEALSGVEAVGDIRGRGHFIATELVADRKTKKPFDGSLKLFMLVRQQALANG
                     LICYPVGGNVDGINGDVIILAPPYNSTDAELTEMVDKMAKSIKQVLAAKGLS"
     gene            8395..9426
                     /locus_tag="PKV23_00150"
     CDS             8395..9426
                     /locus_tag="PKV23_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006933448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HQX83869.1"
                     /translation="MLTVFNETQRLHAPSTFIVSGKPQPIPEKPERIDMLLKGVEQVG
                     SKLVAPPPITMQTVGLVHEQRYLTFLETLLERWSHIPDASDIPLPNIYAMGRSTLAPV
                     SYPESVVGQAGYHLGDGACPVTPTTLQAAMASVASAVHGASLVLGGEKLVYALCRPPG
                     HHAASDVAAGFCYFNNTALATEIFTRAGKRTAILDIDVHHGNGTEAIFYDRADVLTVS
                     IHAHPKRFYPFFWGYENETGRGAGEGFNLNIPMERGTLIKDYCTSLETALRRVSDFKP
                     DVLVLAAGLDIAVDDPFKGFAIATPEFATIGKMIAQIRLPMLVVQEGGYPSPSLGLNL
                     ASLLKGLGA"
     gene            9423..9902
                     /locus_tag="PKV23_00155"
     CDS             9423..9902
                     /locus_tag="PKV23_00155"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQX83870.1"
                     /translation="MTFEVRTMQDNEAQIVAGMVHGLARDLNLNLVPHLTGNILLESL
                     DLVNVVVAAEAGKLLGACLSLMTFSTFRACKGVYVVDLFVDAAARNRNIGEALLRETA
                     RRGKVRGAKFVKLEVDLTNAGGARFYERLGFKKKLEDRLFILEQDGLNYFIAERVTP"
     gene            9899..>10370
                     /locus_tag="PKV23_00160"
     CDS             9899..>10370
                     /locus_tag="PKV23_00160"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HQX83871.1"
                     /translation="MTIKNVGLIGVGLMGHGIGKNILAKGFALNVMAHKNRAPVESLV
                     SLGAKEYASPAEIAKASDLVILCVTGTPQVEEAIYGAKGLASALRDGLIIADCSTAEP
                     ASTIRIAAELKAKGVYFVDTPMTRTPKEAEAGKLGLMVAGPADVLAKIRPVLDCF"
BASE COUNT         2127 a         3250 c         2955 g         2038 t
ORIGIN      
        1 gagcgccaca ggcagggcga tggcaaccga cagcagcgtt gaaaggaacg cctgcttcag
       61 cgtgaacacc accacgcgcg gcagatagtc atcaatatgg aatgcttgcc cgaagccaag
      121 ccagagcagc ggcaggaacc caagcgcaac cgcacccaga atgaatccga gcgcaattgc
      181 cagtgttagt ttccaaaaca ttatttgctc aaggcattca gccactcatc cacccagtcg
      241 cggcggtttt tgaacacctc gtcgggggaa aagagcagcg tctttccggg cgccaccaat
      301 ttgccgaagg cctccggcaa cgcggtgtcc gtggcccctg caggcagcat ccagttggtg
      361 gtggggatga tgtcctggaa cgccggtgtc atcatgaaac ccagaaattt ctgcgccaat
      421 gccttttccg gtgaggcctc aatcagacca gcgacttcaa tttgaagata gtggccctcg
      481 ctgaaggcgg cagcctgata gcgcggggtg ttttccgcca ccatgtgata ggcgggcgaa
      541 gtcgtatagg acaagaccat cggcgcctcg cccttggtga acagcccata ggcctcgctc
      601 cagcccgggg taatcgtcag gattcgcggc tgcagctttt tccattgctc tgctgcccct
      661 tcgccataaa ccgctttcat ccatagcagg aagccaagac cgggcgttga tgagcggggg
      721 tcttccagaa cgatcttctg ggcgggatcg ccattgacga gttcatcaag gctcttcggt
      781 ggagacgcca ttttctccgt gtcatagatc accgcgaaat gcgcatagtc atagggcatg
      841 aaataatcgt cggcccaggc ctccggcgtt ttggccagcc ccttgtccac cccatgtttg
      901 ccgaacaatc cagttccaat ggcctccgcc gtcaggctgg tatctaaccc cagaacgaga
      961 tcggccttgg tgttggcgcc ttccagtttc aggcggttga gcacggcgac accgtcaccc
     1021 aatccgaccc actcgatgct gcagtcgcag gtcttctcga atgcctcctt caccttgccg
     1081 cccgggcccc attcggtcac gaagctttcg taggtgtaga tcgtcaacgt ctctttggcc
     1141 tgggccaagg aagcgaaaat gacggaagcg atgattgtgc tgaaaatctt gagcataata
     1201 cctccaatca gtttcggatg gaggcaggga acgaggggga ttccaggttc cgctcgcaat
     1261 ccctccgccg gcatgatccg gatcaggttc agcgggttgg agcggaaggc tcctctcagc
     1321 cgcctaggca tgtccccgaa aagttgaaga cttttcggaa aaggacaggc ccaaagaagg
     1381 gcggcacccc gttgagagca atcccagacc taaacataag ggcgtgatct ttcaagctgt
     1441 ttttgctaca tggcgggcat gtccagattt gccatcctcc tcggcggcga ccttgtggtc
     1501 accccacgcc tgcgccggca aatcgccggg gcccgcatca ttgccgccga tagcggcatg
     1561 aaacatgcca gcgccctggg gcttatgccg gagctctggg ttggcgattt tgattccgcc
     1621 ggctcggaac tcctgctgga ctatgcgctt gtgccgcgcc agacccattc cgccgacaag
     1681 gatgcaacag acggcgaaat agccgtggct gaagcccttc gcctcggcgc taccgaaata
     1741 gtattgatcg gaggactggg gggcgaaagt gaccatgcca ccgcccacct tggcttggcg
     1801 cttcacctgg cacggtccgg ccaaaaatgc ttcatctcca atggcgacga agaggcttac
     1861 cccctcacag ccggaaccta tgaatttgat ttcggccccc agagccgctt cagcatcgtg
     1921 ccgtggagcg acatcaccgg gctccatctt accggcgtga aatggccgct gaaggacaag
     1981 gcactcaccc ttggcacaag cttcaccatg tcgaacgtcg ccctgggacc tgtgaagctg
     2041 tccttctcct ccggcatcgc cattgccata gcctatccga cgcagacgga ctgacatgca
     2101 ggtcaccatc gaatatctca attggcgcgg cgaaggcgtg ggcggcggcc agacattccg
     2161 caatgttctc ggtggcgaaa ctgtggatgc ggcaacagga caggttcttc aatcctcgcc
     2221 cgagcgcatc gaccccttct gcaaacatta cgtaaaatgc ggcggctgcc agttgcagca
     2281 ttggcagcag cttccttatg aagcctggaa gcgcaatctc atcaccactg ctcttgagaa
     2341 gcgcggcatc aattttcaag taggcgacat tatcgatgcc catgggacag ggcgccggcg
     2401 cgtgagcctc catgtcagga aaagaaatgg cattgtgcag gcgggcttca tggccgcccg
     2461 cagccatgat ctcatcgaca tcgatcagtg ccccattctc gttcccgcct tgcagaactc
     2521 gacagatatc gcccgcaagc tcggcgcaaa actcggcgat tgcgatgtct caatcaaggc
     2581 ggcggacaat ggccttgatg tttcaatcaa ggcggaacgc aaaatcgctg atgagcaggt
     2641 gccgaagttg gcaaccctcg tggaggaatt gaacctcgct cgcctgagca tcaacggaaa
     2701 cttggtggct tcccgcgccg tgcccgttgt gtccatcggc aaggcccttg tgcaggtccc
     2761 gccggggtca ttcctgcaag cgacaaaggc gggcgaagaa gccctggcaa atctggttct
     2821 ggcgtcattg ggaaagtcaa aatccgtggc cgatctcttc tgcggcgttg gccccttcgc
     2881 cctgcgcatt gcggaacggg ccaaggtcac tgccatcgat agcgacaagc cggccatcac
     2941 cagcctggct caggcctcgc gcctcaccac tggcctcagg ccactgaaag ccgaagtccg
     3001 cgatctcatg cgcgagccct tggtagccag tgaattgaaa gaattcgaca cagtggtttt
     3061 tgacccgccc cgcgccggcg cagaggcgca ggcccggcaa ctggccaaat caacagtgaa
     3121 aacaattgtc gccgtgtcct gcgatcccgc cacttttgca agggatgcag aaatcctggt
     3181 gggcggcggc tacaagcaga aatctcttac cgctgtcgac cagttcaaat ggacctccca
     3241 cgtggaaatc gtgggaactt tcggccgtta gtccttggca cgctccacat aggagccatc
     3301 ggcggtttcc acgacgatgc gggtgcccga aacaatatgc ggcggaacca gcgtgcgcac
     3361 gccgttggaa agcagggcgg gcttgaacga cgaagacgcc gtctggttct tgatagcggg
     3421 ttccgtttcc atgatttcca aagtcacgcg ctgcggcagc tcgatggaaa tcggcgcgcc
     3481 gttgaacagc gcgatctggc attccatgcc ttcctgcagg aagggcgcgg catcgcccac
     3541 catgtcttca tgcaggctga tctgctcgaa gctttccgga ttcatgaaca catacatgtc
     3601 gccatccttg tagagatagc tgtgcgtcac ggtttccacg aaggcccgtt ccatggcatc
     3661 cgtcgtcttg tagcgcacca cggttttgac cccgtccgag atgcgccgca tgtcgatggt
     3721 ggttgtgggc gtgcccttgc cgggatgcat gctttccgat ttgaggacaa cgtaaagctt
     3781 gccatcctca tgctcgatga cattgccctt gcgaacgccg cttgcgataa ccttgaccac
     3841 tgtttgaaac cttcatgaat tgctgatttg agttggcggg ggtattagca gcacccggag
     3901 attaagccaa cccctgcaag gatagcccgt aaatgccctg gttttcgccc gaaaggcaca
     3961 aggatagaag gcccttcctg ctggcccgga accgcatttt ggcgggtttc cgccaatggt
     4021 ttgacgagcg ggggtttctg gaggtggatt gcgccgctct gcaggtctct cccggcaatg
     4081 aagcccatct ccatgccttt gccacggacc tgatccagcc cgacggcagc cgtgaacggc
     4141 gctatctcca cacctcgccg gaattcgcct gcaaaaaact gctggccgcc ggcgaaaccc
     4201 gcattttcag cctggcgcat gtcttccgga accgcgagcg aacgctcacc cattccccgg
     4261 aatttaccat gctggaatgg taccgggcca acgagcccta tgccgcaatc atcgacgact
     4321 gcctggcgct ggtgcggctc gcggcaatca tttcagaatc gaaacagttt aactggcgcc
     4381 acaggaaggc tgatccattt gcagaagccg aatggctcag tgttgcctgg gcctttgaaa
     4441 aatacgccgg cgttgatctg ctggcgaccc tgaatggcag tgaatccaat cgcgaggctc
     4501 ttgccaagca atgcccagtg caattcaccg acagggattc ctggtccgac atcttcagcc
     4561 gcattctggt cgaaaaagtc gagcccaatc tgggcaatgg cactgttacg gttctatacg
     4621 aatatcccgc ctgcgaggca gccctggccc gggtctgcac ccacgacccc cgcgtctcgg
     4681 aacgcttcga gctttatgcc tgcggcgtcg aactcgccaa cggcttcggc gaactcaccg
     4741 acccggtcga acagcggcgc cgtttcgaga ccgaaatgaa tttgaaggaa cagctctacg
     4801 gcgagcgtta cccggtagat gaagaactgc tccaggccct gcgcctgatg ccacccgcct
     4861 caggcgtcgc cctgggactg gaccgcctga tcatgctggc gaccggcgcg acccggattg
     4921 gtcaggtgca gtggacaccc gtttcataac ccactgacaa tttgtcattc cggcgtgcgc
     4981 cggaatccac ccgcgccccg tcgccttgca atcccaaagc gattgacagg ccagttccgc
     5041 cgctccctcc ggtgaccaga gccacccggt ttgcgggatc aaatgcctgt ttgatcggtt
     5101 ttacctgtgg aatagactgc attagttgca ccaaaacctt gaatagttgc cggaaaccct
     5161 ggaccatcaa gacccttgcg tcttcctcaa aaatatatct ctcaaggcac ggagtgaaag
     5221 aaacttggct ggaacggcgc ctctggactt tggattgtat gactacatca tcatcggtgc
     5281 agggtcggca ggctgcgttc tggcgaaccg gctgagcgca tcgggccgcc atagggtgct
     5341 gcttctggaa gcgggtggcg aggaccgcaa tccctggatt cacattccaa ttggctacgc
     5401 caagctgttc aacaaccacc actacaactg gatgtatgaa acggagcccg agccggaact
     5461 ggacggccgc aagatttatc agccgcgggg caaggtgctg ggtggaacaa gctccatcaa
     5521 cggactcgtc tatatccgcg gccagaagga ggacttcgac caatgggccc ggatgggcaa
     5581 cactggctgg tccttcgcgg atgtgctgcc ctacttcaag cggtccgagg atcaggagaa
     5641 cggtgcggac gcgtaccacg gggccggcgg gccgctggcc gtttctaacc cgcgcgagcc
     5701 gcacgaactc tgcgaagcct tcatcagggc cggcatggaa cgtggcctgc cgcgcaggga
     5761 ggatttcaac ggcgcggacc aagagggcat cggctatttc cagaccacct cgcgcaacgg
     5821 ccgccgctgc agcacggcgg tgggctatct ccgcccggcg cggggccgca agaacctcca
     5881 tattgtcacc cacgcattgg cgcatcgcat cgtgttcgag ggcaggcggg cagccggcgt
     5941 ggaattcctg caccagggcc gcatcaagct cgcgcgggcg ggccgtgaag tcgtccttgc
     6001 ggcaggtgct atcaactctc cgcagcttct cgaactatcg ggctgcggtc aggccgaacg
     6061 cctgaatggt ttcggcatac cggtcatcct cgatgcgccg caggtgggcg aaaatctcca
     6121 ggaccatctg caggtgcgaa tggtttttcg ctgcaagaag ccaatcaccc tcaatgatca
     6181 gtaccgcaat ctcttccgcc gcgtgggcat tggccttaga tatgccgtgc agcgcaaggg
     6241 cccgctcgcc gttagtgccg gatatggaac ggcctttttc cgatcggatg accgcatcgc
     6301 cacgcccgac atccagatcc acttcatcat tttttccacc gacaggatgg gccagaactt
     6361 gcaccccttc ccgggtttca cggcttcggt gtgccacctc cgtccggaaa gccgcggctc
     6421 aattcacctc aggagcgccg atcccggcca gtcgccatca atccgcgtga actacctgtc
     6481 cacggacgaa gacaggcgtg ccgttgttga tggcctgaaa tttctgcgct ccatcatgca
     6541 ggcccccgcg atgctggact atgcctcaga agagatcgag ccgggccccg catgtgccag
     6601 caaccaggat cttctcgcct attgcagggc aaagggcagc accatctatc atccttccgg
     6661 cacctgccgc atgggggttg atccaggagc cgtcgtctct cccgatctga aggtgaaagg
     6721 cctttctggc ctccgcgttg ccgatggctc cataatgccg caactcgtat ccggcaacac
     6781 caacgcggcc atcatcatga tcggtgaaaa ggcctcggac ttgatcctcg gggaaagcct
     6841 ctgaatgagg cgtctcaact ggctgtggac atcggcttct caaatcctca ccctgaagag
     6901 ccttggcgcg ggccagggcc tcgaagggtc gttgatcgcg gaaacggatg cttcgagacg
     6961 tgcgttgccc gcacctcggc atgaggggtt aagacagtcc tttcgccgcc agcacctgct
     7021 tgatgctctt ggccatcttg tcgaccattt cggtcaattc ggcgtcggtg gaattatagg
     7081 gcggcgccag aatgatcacg tcgccattga tgccgtcaac attgccgccg acgggatagc
     7141 agattaggcc attggccagg gcctgctgcc gcacaagcat gaagagtttc agggagccgt
     7201 cgaatggctt cttggtcttg cggtcggcca ccagttccgt ggcgatgaag tgcccccggc
     7261 cgcgaatgtc gcccaccgcc tcgacgccgg acagggcctc ggcgatcatc ttcttcagct
     7321 tcggctcatt ggcgcgaacc ctggcgacaa gcccctcccg cgccacgatt ttttgaaccg
     7381 caaccgcggc agcgcaacag gccgtgtggc cggtgaaagt atggcccgtc atcggtgcgc
     7441 catgcacccg catgatgggc tccgccaccc ggtccgtata gatcgcagcc cccagcggca
     7501 ggtaacccgc cgcaagcccc ttggccaccg acatgatgtc aggctccacc ccatccgttt
     7561 ccagcgcccg ccacgtgccg cagcggcctg aaccgcacat gacttcatcg gcaatcatga
     7621 gcacgccgta tttgtcgcaa atctcgcgca cccgcctggc atagcccgca ggcgccggaa
     7681 cgcagccgcc ggccgcaccc accaccggtt caaaaatgaa cgccgcgatc ttgtccggcc
     7741 ccaccgccaa gatttcatct tctagttcct gggcgcaggc ttcacccaca ttggccgcga
     7801 tgggccgata ggcattggcc ggagacaggc gcgaaacgtc gagcagcgaa ccctcaaaag
     7861 ccgccttccg ctcaaggaag ccggacaccg acagggcccc gagcgtattg ccatgccagc
     7921 tccgttcgcg cgcaatgaac cggcggcggg acatttcgcc ccgcgccgca tgatattgca
     7981 gcgccagctt caggcagctt tccaccgcct ccgatccgcc cgtcacataa accacatgct
     8041 tcagcgtccc gccgcaaacg ccgcggatga tctcggtcaa ttcttccagc gcatcggtgg
     8101 tgaaattata acggtagcca tgggcaatgc ggtccagctg cgatttgatg gcctcattca
     8161 cttccgcatt cgaatgcccg atgcaataaa ccgcaggccc ccccgagcca tcgatatact
     8221 gcttgccatc cacatcgtgg agatagcttc ccttggccca gacgaccttg ggcagggaac
     8281 cggtggaatg gatgttggga ggaatggcag gcgcggcagt catgttcaaa tccatcttgc
     8341 aaaattggct ggcttcatgc ggtactagca gaaacaagga agaagccgca aatcatgctc
     8401 accgttttca acgaaacgca acgcctccat gcgcccagca cattcatcgt gtcgggcaag
     8461 ccccagccca tccccgagaa gcctgaacgc atcgacatgc tcctgaaggg cgtggagcag
     8521 gtcggctcaa aactggtggc gccgccgccg atcaccatgc agaccgtggg attggtccac
     8581 gagcagcgct atctcacctt cctggaaacc ctgctggagc gctggagcca tattcccgat
     8641 gccagcgaca ttccgctgcc caatatctat gccatgggga ggtcaacctt ggcacccgtc
     8701 agctaccccg aatccgtcgt ggggcaggcg ggctatcacc tgggggacgg cgcctgcccg
     8761 gtcaccccca caaccctgca ggccgctatg gcgagtgtcg ccagcgccgt ccatggcgca
     8821 agcctggtgc tgggcggcga aaaactggtc tatgccctgt gccgcccgcc cggccaccat
     8881 gcggcctccg atgtcgccgc cggcttctgc tatttcaaca atacggcgct ggccaccgag
     8941 attttcaccc gggccggcaa gcgcaccgcc attctcgata tcgacgtgca tcacggcaac
     9001 ggcaccgagg ccatatttta tgaccgtgcc gatgtcctga ccgtgtccat ccacgcccat
     9061 ccgaaacgat tctacccctt cttctgggga tatgagaacg agacagggcg tggcgcgggc
     9121 gaaggcttca acctgaatat cccgatggag cgcggaaccc tgatcaagga ctattgtacg
     9181 agcctggaaa cggccctgcg gcgggtgagt gacttcaaac ccgatgtgct ggtgctggcg
     9241 gcgggcctgg atattgccgt ggacgatccc ttcaagggct ttgccatcgc cacgccagaa
     9301 tttgccacta tcggaaaaat gatcgcgcag attcgattgc ccatgctggt tgtgcaggaa
     9361 ggcggctacc ccagcccaag cctcggcctc aatctcgctt cgctgctgaa gggtttgggc
     9421 gcatgacatt tgaagttcgc accatgcagg acaatgaggc gcagatcgtc gccggcatgg
     9481 tgcatggcct ggcgcgcgac ctgaatctta acctggttcc ccacctgacc ggaaacatcc
     9541 ttctcgaaag cctcgatctg gtaaatgtgg tggtggccgc cgaggcggga aagctgcttg
     9601 gcgcctgcct ttccctcatg acgttttcca cctttcgcgc ctgcaagggc gtttatgttg
     9661 tggacctctt tgtcgatgcc gcagcgcgca accgcaatat cggcgaggcg ctgctccgcg
     9721 aaaccgcacg gcgcggcaag gtgcgcggcg ccaaatttgt caagctcgaa gtggacttga
     9781 ccaatgccgg gggcgcgcgg ttttatgagc gcctcggctt caagaagaaa ctcgaagaca
     9841 ggctcttcat cctggaacag gatggcctga attatttcat cgcggaaagg gttaccccat
     9901 gacaatcaag aacgttggcc tcattggcgt cggcctcatg ggtcacggca ttggcaagaa
     9961 cattctggcc aagggctttg ccctcaatgt gatggcgcac aagaatcgag cacccgtcga
    10021 aagcctggtg tcacttggcg ccaaggaata tgcctcgccc gccgaaatcg ccaaggcctc
    10081 ggacctggtc atcctctgcg tcaccggaac gccgcaggtt gaagaagcca tctatggcgc
    10141 caagggcctg gccagcgcgc tgcgcgatgg cctaatcatc gccgactgtt caacggcgga
    10201 acccgcctcc accatcagga ttgccgccga gctcaaggcc aagggcgtgt attttgtcga
    10261 cacgcccatg acccgcacgc ccaaggaagc cgaagcgggc aagctcgggc tcatggtggc
    10321 ggggccagcc gacgtgctgg ccaaaatccg gcccgtgctg gattgctttg
//
LOCUS       DAORVN010000004         9430 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_3937_length_9430_cov_3.555307, whole genome shotgun sequence.
ACCESSION   DAORVN010000004 DAORVN010000000
VERSION     DAORVN010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 9430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9430
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3937_length_9430_cov_3.555307"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            7..1386
                     /gene="argH"
                     /locus_tag="PKV23_00165"
     CDS             7..1386
                     /gene="argH"
                     /locus_tag="PKV23_00165"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008550290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HQX83872.1"
                     /translation="MTNKMWGGRFKSGPGAIMQEINASIGFDKRLYAQDIAGSKAHGA
                     MLAHQGIISKSDAKEITRGLDQVQAEIEAGTFTFSTALEDIHMNVEQRLKDIIGEPAG
                     RLHTARSRNDQIATDFRLYIRDTVDHLDGQLKDLQLALAEKAEAHAKTIMPGFTHLQV
                     AQPITFGHHCLAYVEMLARDRSRLADARRRMNESPLGAAALAGTSFPIDRAMTAKAMG
                     FDRPMRNSLDAVADRDFVLELLSAAAICATHLSRLAEEIVIWSTTQFRFVKLSDKFTT
                     GSSIMPQKRNADAAELVRAKPGRILGALTSLLVVMKGLPLTYSKDMQEDKEPAFDAID
                     SLSLAIAAMAGMVRDLVPNAEVMRIAAASGYSTATDLADWLVRTLKIPFRDAHHVAGS
                     LVALAEKDGCDLPELKLEDMQEIEKRITRDVYKVLTVEASVASRTSFGGTAPANVARE
                     ARRWLKLLK"
     gene            1397..1504
                     /locus_tag="PKV23_00170"
     CDS             1397..1504
                     /locus_tag="PKV23_00170"
                     /inference="COORDINATES: protein motif:HMM:NF025012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HQX83873.1"
                     /translation="MKLIITLLLLVGITTTLNACGRRGDPEAPQLQQAQ"
     gene            1508..2782
                     /gene="lysA"
                     /locus_tag="PKV23_00175"
     CDS             1508..2782
                     /gene="lysA"
                     /locus_tag="PKV23_00175"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HQX83874.1"
                     /translation="MHHFAYRDGVLHAEDVDLNIIADEIGTPFYCYSSATLERHYRVM
                     AEAFNGTDHFICYAMKANSNQAVIRTMADLGAGMDVVSEGELRRALAAGVPGRKIVFS
                     GVGKTAREMALGLKEGVSCFNVESEPELELLSQVAQRTGQRATISIRVNPDVDAKTHA
                     MITTGKADNKFGISFTRASQVYARATALPGLDVAGIDMHIGSQITDIEPFEQAFRLMA
                     ELATRLKSEGHNIRHLDLGGGLGVPYRGTNDVPPHPDEYAAMVKRTLGHLGLKYVLEP
                     GRMIAGNAGILVSRVIYVKENDGNYFVIQDAAMNDLIRPTLYDAYHEIIPVKKPADKA
                     SEVTADVVGPVCETGDYIAKARRLPTPDPGDLLATMTAGAYGAVQSGTYNTRPLVAEV
                     LVKGSTWSLVRPRQTYEDLLGLDRLALWQAKS"
     gene            2856..5369
                     /locus_tag="PKV23_00180"
     CDS             2856..5369
                     /locus_tag="PKV23_00180"
                     /inference="COORDINATES: protein motif:HMM:TIGR02302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02302 family protein"
                     /protein_id="HQX83875.1"
                     /translation="MAELTQARIERKIAAARLALFWESLWNALCWPLILTLVLAATAL
                     SGLLTQLPDLLRFGLLGLLAVAIVWSLRHVLRITWPTPYAAMRRIEAHSSLFNRPLST
                     SNDTLAGDSLDERSLALWEEHKRRQLAKLSGLKVGAPQSNWRDLDPRSLRVPASLALF
                     AVIFLAQGDGISNLKDSFQVGPAAAQKPVSLDAWLKPPAYTGKAPLLLTSPAQIEKLK
                     TDRNILVAENSGLVLRLDGAKNPRLGFYDLSNPEAELKSLTATTKSTHGLFEAEAKLN
                     VPALIRVFDGGTVLAAWHVSLIPDKPPSVSVVEEPKSENLGSLTVKWKAADDYGVSAI
                     TSQIDLSDNQEDGVGFAGNGVFLFDPPKFPVSLRKSAPREELGSSSADLTAHPWAGLF
                     VDLTLEVSDAAKQTTKSDVKSFKLPERFFTKPLAQALIEQRKTLIMEPDEAGSVEKLL
                     QALLIYPAGLIENSGTQISIAAIVSRIRNVRSHTDVKEAVDLLWQTALSIEEGDLSDA
                     RAELEAARKALERALAEGATPEQLQELMKNLRGAMDRYMQSMKREAEKRMAQGEQNKN
                     RRTPNGKMITEQDLQQMLDTIEKLSQNGANEAAQELLAQLDEILKNLEPGMAQQGGQQ
                     GDSATTEMLNELSELMRRQQELMDQTQRMPGGESGPLDQEGDDPGNRSGNMGSEGLAG
                     EQGNIGRMLEQFMKQLGQNGLQAPPSFGEAGKQMGEAEQSLQQREREQALGAQGEALN
                     QLRAGAQDMARQIQQQGQNGQDNTGREGEARGETHDPLGRPMPYSGDNFGPKENMLPS
                     ELAIRRAREILELLRSRANTPDLPRIDKDYIDRLLRGLY"
     gene            complement(5366..5917)
                     /gene="hpt"
                     /locus_tag="PKV23_00185"
     CDS             complement(5366..5917)
                     /gene="hpt"
                     /locus_tag="PKV23_00185"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012320927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HQX83876.1"
                     /translation="MSDITIDGHRISVLFSEADIAKRIEVLAREIAARKPKNLLVVPV
                     LKGSFIFAADLIRAMHHAGLSPEVDFMILASYRAAQVSSGQVEVLRDIESPIRDRDVM
                     LVDDILESGRTLAFAKDLLMARGASSVMTAVLLDKPGHLAANIKADFCGFSCPDKFVV
                     GYGMDMAHQFRELPFVGHVVPAD"
     gene            complement(5914..6162)
                     /locus_tag="PKV23_00190"
     CDS             complement(5914..6162)
                     /locus_tag="PKV23_00190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX83877.1"
                     /translation="MSIKGEVSNITNSTRKIPWLRFGLNDGGHGEVYTWTLDTGARPL
                     RAGESTSFVTRVASPPETAKNLQIRFAHRDEIGSNPSP"
     gene            complement(6211..6624)
                     /locus_tag="PKV23_00195"
     CDS             complement(6211..6624)
                     /locus_tag="PKV23_00195"
                     /inference="COORDINATES: protein motif:HMM:TIGR02098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HQX83878.1"
                     /translation="MIISCPSCSTHYNFPDQRLTGDGMAISCAACGHSWIESRAIEIV
                     DISPRKVPAVIEHGFEPDSEVKRLVEASRRAKEAFAVKKRQRLGRLRGWAILGACLLA
                     PVAAAAAFPEAVVSLAPVTVRAYDAAGYNVNIYGL"
     gene            6767..7423
                     /gene="ftsE"
                     /locus_tag="PKV23_00200"
     CDS             6767..7423
                     /gene="ftsE"
                     /locus_tag="PKV23_00200"
                     /inference="COORDINATES: protein motif:HMM:TIGR02673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HQX83879.1"
                     /translation="MVRLDSVGLRYGDGPEILKDVSFHLRPGSLCFLTGPSGAGKTSL
                     LRLLFLALKPTRGIINIFEKDVSQVSRWELPLLRRRIGVVFQEFRLLDHLTTFDNVAL
                     PLRVMGQREDSYRSDVLDLLNWVGLADQAQAYPPVLSGGEKQRAAIARAVISKPELLL
                     ADEPTGNVDPVLAKRLLHLFMELNRLGTTVFLATHDLKLVSQGKGSVLHLEKGILKAA
                     "
     gene            7420..8331
                     /locus_tag="PKV23_00205"
     CDS             7420..8331
                     /locus_tag="PKV23_00205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX83880.1"
                     /translation="MTRQKKFRKGPIIPPEAAPLKALTVTMTVMCYLACLAIGALILI
                     DRAVESWTSGLSREVTVQIRLMQNADIDKELAKAKVLLETTVGVTKVEVLERDAGAKL
                     LEPWLGTGSLDDLPIPRLIRVTIDEAAPPDFVALDNQLKKSVSGASLDTHRRWEVELT
                     RMARALSTLSYAILALICISAIAMVIFATRTVLDANRQVVDVLHLVGAKDSYIAHQID
                     SRFLRTGFMAGLLGVGLGVLTFFLLGLAGTMGIGGVADASRGLLFAPPEIAVWSYGLL
                     ISVPIIATLICLVTSRVALIRMLRSVL"
     gene            8328..9074
                     /locus_tag="PKV23_00210"
     CDS             8328..9074
                     /locus_tag="PKV23_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012110499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HQX83881.1"
                     /translation="MIAFRSLLFNIAFYVNLALFMLLGAGFFLAPRKVALRALQAWAR
                     TSLWLLRVICGTQIEVRGKENIPDGPVLVAGKHQSVWETFAILPCLDDPCMVLKRELS
                     WIPFFGWFIFKFRMIRLERGAGSAALRKLMARGVEELAAGRQIVIMPEGTRQAPDAPP
                     AYKPGAAALYGTLGCPCLPFAVNSGLFWPRRKFLRYPGTIVLEFLPLIPAGLPRKAFQ
                     ERLQDDIETTTAKLVAEGREAVRIKNISRL"
     gene            9119..>9430
                     /locus_tag="PKV23_00215"
     CDS             9119..>9430
                     /locus_tag="PKV23_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide reductase N-terminal alpha
                     domain-containing protein"
                     /protein_id="HQX83882.1"
                     /translation="MDKSVGILDPISQEIWDMKYRFKDVGGAIHDQTIEDTWWRVARA
                     VAEAEKPALRETWASSFYEAFSDFRFLPAGRIVAGAGTKRSVTLFNCFVMGTIDDSML
                     GI"
BASE COUNT         1921 a         3000 c         2669 g         1840 t
ORIGIN      
        1 tttccaatga ccaataaaat gtggggcggc aggttcaaat ccgggcccgg cgccatcatg
       61 caagagatca atgcttccat cggctttgac aagcgcctct atgcccagga cattgcaggt
      121 tcgaaggccc atggcgcaat gctggcccac cagggcatca tttccaagtc tgatgcgaaa
      181 gaaattaccc gtggcctcga ccaggtacaa gccgagattg aagctggaac gttcaccttt
      241 tcgacagcgc tcgaagacat ccacatgaat gttgaacagc gcctgaaaga catcatcggc
      301 gaacccgccg gaaggcttca tacggcacgt tcgcgcaatg accagatcgc cacggatttc
      361 cgcctttata tccgtgacac cgttgaccat ctcgatggcc agttgaagga cctgcaattg
      421 gcgctcgccg aaaaagccga ggcccatgcc aagaccatca tgcccggctt tacccatctg
      481 caggtggccc agcccatcac tttcggccac cactgcctcg cctatgtgga aatgctggcg
      541 cgcgaccgct cccgtcttgc cgatgcaaga cggcggatga atgaaagtcc tctgggtgcc
      601 gcggcacttg ccggcacatc atttccaatt gaccgcgcca tgacggccaa ggctatgggc
      661 tttgaccggc ccatgcgcaa ttcgctggat gcggtggccg accgtgattt tgttttggag
      721 cttctctccg ccgctgccat ttgcgccacc catctctcgc ggctggcgga agagattgtc
      781 atttggtcca ccacgcaatt ccgtttcgtg aagctgtcgg acaagttcac caccggctcc
      841 tccatcatgc cgcaaaagcg caatgccgat gcggccgagc tggtgcgcgc caagcccggg
      901 cggatcctcg gcgcgctcac atccctgctt gtcgtcatga aggggcttcc cctcacctat
      961 tccaaggaca tgcaggaaga caaggagccg gcctttgatg caatcgacag cctatcgctg
     1021 gccattgcgg cgatggccgg tatggtgcgc gatctcgtgc ccaatgcgga ggtcatgcga
     1081 attgccgcgg caagcggata ttcaaccgcc accgatcttg ccgactggct cgtccgtaca
     1141 ctgaagatac cgttccgcga cgcccaccat gtggcggggt cgcttgtggc actggcggaa
     1201 aaggacggct gcgacctgcc cgagctgaaa ctggaagaca tgcaagagat cgaaaagcgc
     1261 attacaaggg atgtctacaa ggtgcttacc gtcgaagctt ccgttgcctc gcgcacgagt
     1321 tttggcggaa ctgcaccggc aaatgtggcg cgcgaagcca ggcgctggct gaaactgttg
     1381 aaataggggg aatcacatga aactcatcat tacgctgctg ctcctggtgg gcatcaccac
     1441 cacgctgaac gcttgcgggc ggcgcggcga tccggaagcg ccacaattgc agcaggccca
     1501 gtaatccatg catcattttg cctatcgtga tggcgtgctc catgcggagg atgttgatct
     1561 caacatcatt gccgatgaaa ttggcacgcc cttctactgc tattcctccg ccaccctgga
     1621 gcgccactac agggtgatgg cggaagcctt caatggcacc gaccatttca tctgttacgc
     1681 catgaaggcc aattccaacc aggctgtgat ccggaccatg gccgatctcg gcgcaggcat
     1741 ggatgtggtc tccgaaggcg agcttcgccg cgcccttgcc gccggcgttc ccggccgcaa
     1801 gatcgtcttt tccggcgttg gaaaaaccgc ccgcgaaatg gccctcggcc tgaaggaggg
     1861 cgtctcctgc ttcaatgtgg aatcagagcc ggaactggaa ctgctttccc aagtggccca
     1921 gcgcaccggc cagcgcgcca ccatttccat ccgcgtcaat cccgatgtcg atgccaaaac
     1981 ccatgccatg ataaccaccg gcaaggccga caataagttc ggcatttcct tcacccgcgc
     2041 ctcgcaggtc tatgcccgcg ccaccgccct tcccggactt gatgtcgccg gcatcgacat
     2101 gcacatcggc agccagatca ccgacatcga acccttcgaa caggctttca ggctgatggc
     2161 cgaactcgca acgcggctga aatccgaagg ccacaacatt cgccatctcg atctgggcgg
     2221 aggcctaggc gtcccctacc gaggcaccaa tgacgtgccg ccccaccccg atgaatatgc
     2281 cgctatggtg aagcggacgc tcggccacct cggtttgaag tatgtcctcg aacctggccg
     2341 catgattgcc ggaaatgctg gcatcctggt gtcgcgggtg atctatgtga aggaaaacga
     2401 tggcaactat tttgtcatcc aggatgcggc gatgaatgat ctcatccggc ccaccctcta
     2461 tgacgcctat cacgagatca ttccggtgaa gaagcccgcc gacaaggctt cggaggtcac
     2521 cgccgatgtc gtgggcccgg tctgcgaaac cggagattac attgccaagg cacggcgcct
     2581 gcccacgccc gatccgggcg atctgctcgc caccatgacg gccggcgcct atggcgccgt
     2641 gcagtccggc acttacaaca cccggccgct cgtggccgag gtcctggtaa agggcagcac
     2701 ctggtcgctg gtcaggcccc gccagaccta tgaggatctc ctcgggctgg accgccttgc
     2761 cctgtggcag gcgaagagct gacgcttcca ttcattaaaa tgtgataagt tcccttgggc
     2821 atatttccgc cgaggtgtgc tagtatcttc ccggcatggc tgaactcaca caggctcgca
     2881 ttgagcgtaa aattgcggca gcgcgcttgg ccctgttctg ggaaagcctg tggaatgccc
     2941 tgtgctggcc cctcatcctg acgcttgtat tggccgcaac ggcactttcc ggcttgctca
     3001 cccaactccc tgatctgctg cgctttggcc tcctggggct tctggccgta gcaattgtct
     3061 ggtcgctgcg gcatgtactg agaatcacct ggcccacccc ctatgccgcc atgcgccgga
     3121 tcgaggccca ttccagtctt ttcaaccgcc cgttgtccac cagcaatgat acgcttgccg
     3181 gggacagcct cgacgaaaga agtctcgccc tgtgggaaga acacaagcgc cgccaattgg
     3241 ccaagctctc cggcttgaag gttggagccc cgcaatcaaa ctggcgcgac cttgatcccc
     3301 gctcgttgcg ggtgcccgca agcctcgcct tgttcgcagt tattttcctc gcccagggcg
     3361 acggcatctc aaatctcaaa gacagctttc aggtgggccc ggctgcggcc cagaagcccg
     3421 taagcctcga tgcctggctg aagccgccgg cctatactgg aaaagccccg ctgctcctca
     3481 ccagcccggc ccagattgag aagctgaaaa ccgatcgcaa cattcttgtg gccgaaaaca
     3541 gtggcctcgt tctccgcctc gacggcgcga agaatccgcg gctcggcttt tatgatttgt
     3601 caaatccgga ggctgaactg aaaagcctga cggcaacaac caagtccacc catggcctgt
     3661 ttgaggccga agccaagctc aatgtgcccg ccctcatcag ggttttcgac ggcggcaccg
     3721 tactggcagc ctggcatgtt tcgctcattc ccgataagcc accaagcgtt tccgtcgtcg
     3781 aggaaccaaa atccgaaaat cttggctcac tcacagtgaa gtggaaagcc gccgatgact
     3841 acggagtgtc cgcgatcacc tcgcagatcg acttgtctga caaccaggag gatggcgtgg
     3901 gctttgccgg caacggcgta tttctgttcg acccgccaaa gtttcccgtg agcctgcgca
     3961 aatccgcgcc gcgtgaagaa ctgggctcaa gcagtgccga tctcaccgcc catccctggg
     4021 ccggtttgtt cgtggacctc acccttgaag tcagcgacgc cgcaaaacag accaccaaaa
     4081 gcgacgtgaa gtcattcaag cttcccgagc ggttcttcac caaacccctg gcccaagccc
     4141 tgatcgagca gcgcaagacc ttgatcatgg aacccgatga agccggaagt gtggaaaagc
     4201 tgctgcaggc tctcctgatc taccccgcag gcctcatcga aaacagcgga acgcaaatat
     4261 caatcgcggc aatcgtatcg cgcatcagga atgtgcgcag ccacaccgat gtgaaagagg
     4321 ctgtcgatct cctctggcag acggccctga gcatcgagga aggcgatctc tccgatgccc
     4381 gcgccgaact cgaagccgcc cgcaaggcgc tggagcgcgc cctggccgaa ggcgcaacac
     4441 ccgaacagtt gcaggaattg atgaagaacc tgcgcggcgc catggaccgc tacatgcaat
     4501 ccatgaagcg cgaggccgaa aagcgcatgg cccagggcga gcagaacaag aaccgccgca
     4561 cccctaacgg caagatgatc accgagcagg acttgcagca aatgctcgat accattgaaa
     4621 agctgagcca gaacggagcc aatgaggcgg cccaggaatt gctcgcccag cttgatgaga
     4681 ttctgaaaaa tttggagccc ggcatggccc agcaaggcgg acagcaaggc gattcggcca
     4741 cgaccgagat gctcaacgag ctctcggaac tgatgcgccg ccagcaggaa cttatggatc
     4801 agacccagcg catgccgggg ggcgaaagcg gacctcttga tcaggaaggc gatgatcccg
     4861 gtaaccgcag cggcaatatg ggctctgagg ggcttgccgg cgaacagggc aatatcggcc
     4921 gcatgctcga gcagttcatg aagcagttgg gccagaacgg cctgcaggcg ccgccctcat
     4981 tcggcgaggc cggaaaacag atgggcgaag ccgaacaatc cctccagcag cgcgaacgtg
     5041 aacaggccct tggcgcccag ggcgaagccc tcaaccagtt gcgtgccgga gcccaggaca
     5101 tggcccgcca gattcagcag cagggtcaga acggccagga caataccggc cgggaaggcg
     5161 aagcccgcgg cgaaacccat gatcccctcg gccggcccat gccctatagc ggcgataact
     5221 tcggcccgaa ggaaaacatg ctgccctcgg aacttgccat ccgccgcgcc cgggaaatcc
     5281 tcgaactcct gcgctcgcgc gccaacaccc cggacttgcc gcgcatcgac aaggactaca
     5341 tcgaccgtct gctgcgcggg ctctactaat ccgccggcac cacatgcccg acaaagggca
     5401 gctcgcggaa ctggtgcgcc atgtccatgc cataacctac gacaaatttg tcgggacatg
     5461 agaagccgca gaaatccgcc ttgatattgg cggcgaggtg gccgggcttg tcgagcagca
     5521 ccgccgtcat cacgcttgaa gcgccgcgtg ccatcagcag atccttggca aaggccagcg
     5581 tccggcccga ttcgaggata tcatcaacca gcatcacatc ccggtccctg atcggcgatt
     5641 cgatgtcgcg caggacttca acctggcccg aggaaacctg ggccgcccgg tagctggcca
     5701 gaatcatgaa atccacctcc ggcgaaaggc cagcatgatg catggcccgg atcaggtccg
     5761 ccgcgaagat gaagctgcct ttcagaaccg gaaccaccaa gaggttcttc ggctttcgcg
     5821 ccgcgatttc gcgggccagc acttcgatgc gtttggcaat gtcggcttcc gagaacagga
     5881 ccgaaatgcg gtggccgtcg atggtaatgt cgctcatggg ctagggtttg agccgatttc
     5941 atccctgtgt gcaaagcgaa tttgcaggtt tttcgcagtt tccggcggcg aagcgacgcg
     6001 ggtcacgaaa ctggtggatt cacccgcccg aagcggcctt gcccctgtat ccagagtcca
     6061 ggtgtaaact tccccatggc cgccgtcgtt caggccaaaa cgcagccacg ggatcttccg
     6121 ggtgctgttg gtgatattgc tgacctcgcc tttgatgctc aaaacacgga cgccgtcaac
     6181 aagggcatgc tgctgttcaa cccggcggat ctaaagcccg tagatattga cattgtagcc
     6241 cgcggcgtca taggcccgca ccgtgaccgg cgcgaggctc accacggctt cggggaaagc
     6301 ggcggcggcg gcaaccggcg caagcaggca tgcgcccaga atggcccagc cccgcagacg
     6361 gcccagccgc tggcgttttt tcaccgcaaa ggcttccttg gcccgccgcg atgcttcgac
     6421 caggcgtttc acttcgctgt ccggctcgaa gccatgctcg atgacggccg gcaccttgcg
     6481 tggtgaaata tcgacgattt cgatggcccg gctctcgatc cagctatggc cgcaggcagc
     6541 acaactgata gccatgccat cgcccgtcag gcgctgatcc ggaaaattgt agtgtgtcga
     6601 gcaggaagga caagaaatta tcatctgaaa atcatatcat ccattgattc gcccgggccc
     6661 tccgcctaaa gcccaagtac ccttgaattg ttaacagcaa tcagggttaa tgcggcgtta
     6721 agaccgggcg tggtgtgaca gctatttcgg cttggagttc ggttaagtgg ttcgtcttga
     6781 cagtgtgggc ctgcgttacg gcgatgggcc cgaaattttg aaggacgtca gttttcacct
     6841 gcggcccggt tccctgtgct ttctcaccgg gccttcggga gcaggcaaga cctcgcttct
     6901 gcgcctgctg ttcctcgccc tgaagccgac ccggggcatc attaacatct ttgaaaagga
     6961 tgtctctcag gtgagccgct gggaactgcc gctgctccgc cgccgaattg gtgtggtgtt
     7021 ccaggagttc cgcctgctcg accatctcac cacctttgac aacgtggccc tgccgctccg
     7081 tgtcatgggc cagcgcgagg acagctaccg ctccgacgtg ttggacctgc tcaactgggt
     7141 ggggctcgcc gaccaggctc aggcctatcc gccggtgctg tccggcggcg agaagcagcg
     7201 cgccgccatc gcccgcgccg tcatttcaaa accggaactg ctgctcgccg acgagcccac
     7261 cggaaacgtc gatccggttc tggccaagcg cctgctccat ctcttcatgg agctgaaccg
     7321 cctgggcacc accgtgttcc tggcaaccca tgatctcaag cttgtctcgc aaggcaaggg
     7381 ttctgtgctt cacctcgaaa aaggaatatt gaaagccgca tgacgcgcca gaaaaaattc
     7441 aggaagggtc ccatcatccc gcccgaagcc gctcccctga aggcgctcac cgtgaccatg
     7501 acggtgatgt gctatctcgc ctgccttgcc atcggcgccc tgatcctgat tgaccgcgcc
     7561 gtggaatcct ggaccagcgg cctgtcgcgc gaggtcaccg tgcagatcag gctcatgcag
     7621 aatgccgaca ttgacaagga actggccaag gcgaaagtcc tgctcgagac aacggttggc
     7681 gtgaccaaag ttgaggtgct tgaacgcgat gccggcgcca agcttctgga gccatggctc
     7741 ggaaccggca gtctcgacga cctgcccatc ccgcgcctca tccgcgtgac cattgatgag
     7801 gccgcaccgc ctgattttgt tgccctcgac aaccagctga aaaagtccgt gagcggcgca
     7861 agtctcgata cccaccggcg ctgggaggtg gaactcaccc gcatggcaag ggccctttca
     7921 actctctcct atgccattct tgccctcatc tgcatatcag ccattgccat ggtgatcttt
     7981 gccacccgca ccgtgcttga cgccaaccgc caggtggtgg acgtgctgca tctcgtgggc
     8041 gccaaggaca gctatatcgc ccaccagatc gacagccgtt ttctccgcac cggcttcatg
     8101 gccggattgc tgggcgtggg ccttggggta ttgacctttt ttcttctcgg ccttgccgga
     8161 acaatgggca taggcggggt tgccgatgcc agccgcgggc ttctgttcgc gcctccagaa
     8221 atcgccgttt ggagttacgg ccttttaatc agtgtaccga tcattgccac gttgatctgc
     8281 ctcgtcacct cgcgcgtagc gctcatccgc atgttgagaa gcgtgctgtg attgcctttc
     8341 gctcgctgct tttcaatatt gccttctatg tgaaccttgc gcttttcatg ctgctggggg
     8401 cgggattttt cctggccccg cgcaaagttg cgctgcgcgc ccttcaggcc tgggcgcgta
     8461 cctcgctgtg gctgttgcgg gtgatctgcg gcacgcaaat cgaggtgcgc ggcaaagaaa
     8521 atattccaga cggcccggtt ctggtggcgg gcaagcatca atccgtgtgg gaaaccttcg
     8581 ccatccttcc ctgtctcgat gatccctgca tggtcctgaa acgggagttg tcgtggattc
     8641 cgttcttcgg ctggttcatc ttcaaattcc gcatgatccg gttggagcgc ggcgccggct
     8701 ccgccgccct gcgcaagctt atggcaaggg gcgtcgagga gctcgctgcg ggccgccaga
     8761 tcgtcattat gccggaaggt accagacagg cccccgatgc gccccccgcc tacaagcccg
     8821 gcgctgcagc cctctacggc accttgggct gcccctgcct gccctttgcc gtgaattccg
     8881 gcctgttctg gccccgccgc aaattcctcc gctatcccgg caccatcgtc ctggaattcc
     8941 tgccgctcat ccccgcaggc cttccccgca aggccttcca ggaaagactc caggatgaca
     9001 tcgaaaccac cacggccaag ctcgtggccg agggtcgcga ggcagtacga atcaagaaca
     9061 tttctcgact ctgaccacac tttgcgatac tatcgccgca ggagtgaccg aggtcgtcat
     9121 ggataaaagc gttggtattc tcgatcccat ctcgcaagag atctgggaca tgaagtatcg
     9181 gttcaaggac gtgggtggcg ccatccatga ccagaccatc gaggacacct ggtggcgcgt
     9241 tgcccgcgcc gtggccgagg ccgaaaaacc ggccctgcgc gagacctggg cctcttcgtt
     9301 ctatgaagcg ttttcggatt tccgcttttt acccgccggc cgcattgtcg ccggtgctgg
     9361 caccaagcga agcgtcaccc tgttcaactg cttcgtcatg ggcaccatcg atgactccat
     9421 gctcggcata
//
LOCUS       DAORVN010000005         8549 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_4663_length_8549_cov_3.466565, whole genome shotgun sequence.
ACCESSION   DAORVN010000005 DAORVN010000000
VERSION     DAORVN010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 8549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8549
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4663_length_8549_cov_3.466565"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="PKV23_00220"
     CDS             complement(<1..315)
                     /locus_tag="PKV23_00220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX83883.1"
                     /translation="MARRGRITPRFTIREYEDRDESQVVELVRELQAYEAPMYKWGKA
                     PEDIGPWYIAESKKWCAKNDGTILVAECDGTLLGYATLLASCEADGTGDEIAYTYAHV
                     ADL"
     gene            complement(294..539)
                     /locus_tag="PKV23_00225"
     CDS             complement(294..539)
                     /locus_tag="PKV23_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008875056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HQX83884.1"
                     /translation="MAASSTPHFQNDMGLSKIEIGAREFMCVGAKPPFDHPHVFLNMG
                     GAEEIVCPYCSTLFRHSAKLKPNEASPAAAVWHDEAA"
     gene            592..1359
                     /locus_tag="PKV23_00230"
     CDS             592..1359
                     /locus_tag="PKV23_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013418709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQX83885.1"
                     /translation="MHKFNSDSVEIAFDDAGQGEPVLLIHGFASNGRVNWVDTGWVKT
                     LVDGGFRVITMDNRGHGESEKLYNPDLYSAAEMAEDARRLLDHLGISRAHVMGYSMGA
                     RIAAFLTISHPSRVGAAIFAGLAARMITGVGGGAEIARGLEASSLLDVTDVGARAFRI
                     FAEQTRGDLKALAACIKSSRVKITEAMLATIKNPVLVVVGEKDDVAGEIGPLVAAIPG
                     AAGVVLPRRNHMNAVGDKQYKEAVLTFLGSHLLGIDN"
     gene            1421..2248
                     /gene="cysE"
                     /locus_tag="PKV23_00235"
     CDS             1421..2248
                     /gene="cysE"
                     /locus_tag="PKV23_00235"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013166827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HQX83886.1"
                     /translation="MRQSTKPHVVQAIDPVWDRMRREAEEIARKEPVLGGFIFSAVLN
                     HSTFERALVHRLAQRLGSVDLSAELVVQAFEDAMEEEPEIGFAARADIIATFERDPAC
                     HRYVDPLLYFKGFQALQAHRMAHRLLHMGRTDFAKYLQSRSSMISSVDINPSAKIGKG
                     IMIDHGHDIVIGETSVIEDNVSILQGVTLGGTGKETGDRHPKIRQGVLIGAGAKILGN
                     IEIGHCSRVAACSVVLHDVPPNRTVAGVPAKIVGFAGCEEPARAMDHQVQGVADLLE"
     gene            2288..2497
                     /locus_tag="PKV23_00240"
     CDS             2288..2497
                     /locus_tag="PKV23_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008547690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3126 family protein"
                     /protein_id="HQX83887.1"
                     /translation="MKPEELAKLTTYFRKTFGSPSIAVRKRPQKTDSCEVYIGEEFIG
                     VISKDEEDGELSYHFSMAILDIDLE"
     gene            complement(2573..3274)
                     /locus_tag="PKV23_00245"
     CDS             complement(2573..3274)
                     /locus_tag="PKV23_00245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX83888.1"
                     /translation="MVSHHLNYLLQRRRERSLGFVLLFLLMTFFSLAGVVLAIYFVPN
                     PSLLFGRPEIGAPSTSVVVATLEDTKFTLPETIVAKIDKPILGNATRIDLRLPWPFDR
                     QQLNIVHALPTDLHNWVLVTLEPRLGRTGLEERMAPIYSVYLSPDESEEAGLKLRRFK
                     ADSPYKDSELLVAPLGEVIRCDKTPSVLGPIICERLWPFSQGVMARVRFSRQRLAEWK
                     DMDETAQILLKEFAR"
     gene            3380..3685
                     /locus_tag="PKV23_00250"
     CDS             3380..3685
                     /locus_tag="PKV23_00250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX83889.1"
                     /translation="MDMVYLAYLFAVAVGIVTAGIAASLWTWAAGEEPRFALLLEPSA
                     VAPLRALVIVVSVPLLLLLAAWRYVGSASGAMLLVAASLGWSFLQGVFILTRIFGVT"
     gene            3699..4226
                     /locus_tag="PKV23_00255"
     CDS             3699..4226
                     /locus_tag="PKV23_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HQX83890.1"
                     /translation="MALHILDGIAPELPAEGSCWIAASADVIGRVRLERNASIWFNAV
                     LRGDNELISVGEGSNVQDGCVLHTDMGSPLTVGRNCTIGHKVMLHGCTIGDNTLVGIG
                     SIILNNAVIGKNCLIGAHSLISERKVIPDNSLVMGSPGKVVRELDAEAIARLSAAAAS
                     YVRNWQRYAKGLRPV"
     gene            complement(4223..4984)
                     /locus_tag="PKV23_00260"
     CDS             complement(4223..4984)
                     /locus_tag="PKV23_00260"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HQX83891.1"
                     /translation="MNPGKVSPKVTSGRSAGSNLHRKLAQSIGNQILSGVYAPGAILP
                     NEADWCRIYGASRTTVREAVKTLTAKGLLKSRTKIGSRVEPRENWNILDRDVLAWHMA
                     SMNPQEFFASVQEVRKILEPEIAALAALNRTPQQLRAITEAFEDMRKARPGRSSVVPD
                     VRFHLALLAAANNVLLSPFGIMIEAALTNMFEFTSTHNAEPDMFIPKHEGILKAVARG
                     NANAARKAALSLLTDTDQTIAQVPSKARKARKAKS"
     gene            5189..6187
                     /gene="chvE"
                     /locus_tag="PKV23_00265"
     CDS             5189..6187
                     /gene="chvE"
                     /locus_tag="PKV23_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019965563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HQX83892.1"
                     /translation="MAQGKGLIGISMPTKSSTRWISDGESMVKEFTALGYDSDLQYGE
                     DDIPNQLAQIENMVTKGAKVLIIAAIDGSTLSDILRKAQEAGAKVFAYDRLITKTADV
                     DYYTTFDNFGVGVLQGNSLIKGLKERFAGVKPWNVELFGGSPDDTNAFYFYDGAMSVL
                     QPLIDAGDIAIVSGQMGMDKVGTLRWDGAVAQARMDNLLSANYTDKKVHGVLAPYDGL
                     SRGIISSLKGVGYAPGPEMPIVTGQDAEVPSVKAMMAGEQYSTVFKDTRELAAVTAKM
                     VDTVLQGKEPEINDTKTYNNEVKVVPSYLLTPHDVGKDDIEKLLVGSGYIKAEDLQ"
     gene            6305..>7552
                     /gene="gguA"
                     /locus_tag="PKV23_00270"
     CDS             6305..>7552
                     /gene="gguA"
                     /locus_tag="PKV23_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013533633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HQX83893.1"
                     /translation="MQPILEMRDITKTFPGVKALTDVNLSVLPAEIHAVVGENGAGKS
                     TLMKVLSGVYPAGTYDGAIHFDGVERKFGTIADSENSGIIIIHQELALVPLLSIAENI
                     FLGHETATNGIINWFETFARTKELLKKVGLNEHPNTLITNIGTGKQQLVEIAKALNKK
                     VKLLILDEPTSSLNETDSNALLALLKEFKAQGISSILITHKLNEVLKVADRITVLRDG
                     MTVDTLDTHTAKVSEDRIIKAMVGRELTDRFPPRAAKIGEPIFEVRNWSVYHPLHAEK
                     QMIKGVDLIARKGEVVGIAGLMGSGRTEFAMSVFGGAYGQKITGEVLIHGKPANVSTI
                     PRAMAAGLAYVTEDRKTYGLVLIDHIKHNVTLANLEGVSSYAVVDESAELKVANKYRK
                     ELGIRSSGVFQTTDGAVVMVGAFK"
     assembly_gap    7553..7562
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7563..7873
                     /locus_tag="PKV23_00275"
     CDS             <7563..7873
                     /locus_tag="PKV23_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008834684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQX83894.1"
                     /translation="LSGGNQQKVVLSKWLFSGPDVLILDEPTRGIDVGAKYEIYGIIN
                     RLAEEGKAIIVISSEMQELLGISDRIYVMNEGRFVAEMPAKEATQEKIMRAIMKSWEG
                     "
     gene            7875..>8549
                     /locus_tag="PKV23_00280"
     CDS             7875..>8549
                     /locus_tag="PKV23_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012385981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HQX83895.1"
                     /translation="MTDTAVHSQTRNDMLRFVKSNIRDYALLLSLLVIMIFFQVTTDG
                     TLFKPVNMTNLILQNSYIVIMALGMLLIIVAGHIDLSVGSVSGFVGAVAAVMMVPWKM
                     DPFVTMLACLALGAAIGGAQGYFVAYHRIPAFIVTLAGMLIFKGLALTVLGGASVGPF
                     PREFQLLSSGYVPDVFNITFMGGPFNLLALIIGAVVTATIIYFNIKERREQQAHGLAE
                     EPRIIYL"
BASE COUNT         1749 a         2403 c         2605 g         1782 t
ORIGIN      
        1 gagatcggca acatgggcgt aggtgtaggc aatctcgtcg ccggttccat cggcttcgca
       61 actggcaaga agcgtggcat aacccagcag cgtgccatcg cactcggcaa caagtatcgt
      121 gccatcattc ttcgcgcacc atttcttgct ctccgcgatg taccatgggc cgatatcctc
      181 cggcgccttg ccccatttgt acatgggagc ttcgtaggcc tgcaattcac gcacaagctc
      241 gacaacctgc gattcatcgc ggtcttcata ttctcggatg gtgaagcggg gagttatgcg
      301 gcctcgtcgt gccatacggc ggctgcggga ctggcttcgt tcggtttgag tttggcgctg
      361 tgcctgaaca gggttgagca gtaggggcag acgatttcct cggcccctcc catgttcaga
      421 aatacatggg ggtggtcaaa gggcggcttg gcgccgacgc acatgaattc cctggcgccg
      481 atctcgatct tggaaagccc catgtcattc tggaaatgcg gggtggaaga agctgccatg
      541 ggaacctcga aataacttgt gagggatgcc agatagcata tcctgccggg catgcacaaa
      601 ttcaattcgg acagtgtgga aatcgccttc gatgacgcgg ggcagggcga gccggtgctt
      661 ctcatccatg gttttgcctc caacggccgg gtgaactggg ttgacacggg ctgggtcaag
      721 accctggtgg acggcgggtt ccgggtcatc accatggaca atcgcggcca tggggaaagc
      781 gaaaagctct ataatcccga cctctattcg gcagcggaaa tggctgagga tgccaggcgc
      841 ctactggacc atctcggcat ttcccgggcc catgtcatgg gctattccat gggcgcgcgg
      901 atcgcggcat ttctcacgat aagccatccc tcccgggtgg gagcggcgat tttcgccggc
      961 ctggccgcgc ggatgatcac cggggttggc ggcggggcgg agattgcccg gggacttgag
     1021 gcctcgtcgc tcctggacgt gaccgatgta ggggcccgcg ccttccggat atttgccgaa
     1081 cagacccgcg gcgacctgaa ggcgctcgcg gcctgcatca agtcctcgcg ggtgaagatc
     1141 accgaggcca tgcttgcaac catcaaaaat ccggtcctcg tggtggtggg tgaaaaggac
     1201 gatgtggcgg gggaaatcgg cccgctcgtg gcggccattc cgggggctgc cggcgttgta
     1261 ttaccaaggc gcaaccatat gaacgcggtc ggcgacaagc agtacaagga agcggtttta
     1321 acctttttgg gcagccattt actgggaatt gacaattaac cggggcactc cccatctaga
     1381 gggatatctg ctatagatgc ggtgccgagg agaatgaacc atgcgccagt ccaccaagcc
     1441 acacgtcgtt caggccatcg atcccgtctg ggaccggatg cggcgggaag ccgaggaaat
     1501 cgcccgcaag gaaccggttc tcggcggctt tatcttttcg gcggttctca atcacagcac
     1561 ctttgagcgc gcgctggtgc accgcctggc gcaacgcctg ggcagtgtgg atctgagcgc
     1621 cgaactcgtg gtccaggcct ttgaagatgc gatggaagaa gagccggaga tcggctttgc
     1681 ggcccgggcc gacattatcg ccactttcga gcgcgatccc gcctgccacc gctatgtgga
     1741 cccgctgctc tatttcaagg gctttcaggc cctgcaggcg caccggatgg cgcaccggct
     1801 cctgcacatg ggtcgcacgg actttgcgaa gtacctgcag agccgctctt cgatgatttc
     1861 gagcgttgac atcaatcctt ccgccaaaat cggcaagggc atcatgatcg atcatggcca
     1921 tgacatcgtg atcggtgaaa cctcggtgat cgaggacaat gtctccatcc tgcagggtgt
     1981 cacccttggc ggcaccggca aggaaaccgg cgaccgccat cctaaaatca ggcaaggcgt
     2041 gctgatcggg gcgggagcca agattctcgg caacatcgaa atcggccatt gctcgcgggt
     2101 cgccgcctgc tcggtcgtgc ttcacgatgt gccgcccaac cgcacggttg ccggcgtgcc
     2161 ggccaagatc gtcggctttg ccggttgcga agagccggcg cgggccatgg accatcaggt
     2221 tcaaggcgtt gcggatctgc tggaataagg ctagacctcg gtcaatcgcc cttaggagct
     2281 gagccccttg aagcctgaag aacttgccaa gctgaccacc tatttccgca aaaccttcgg
     2341 ctcgccgtcg attgcggtgc gcaagcgccc gcagaagact gactcatgcg aagtctatat
     2401 cggcgaagag ttcatcggcg tgatatccaa ggatgaagag gacggcgagc tgtcctatca
     2461 tttttccatg gccatcctgg atattgacct ggagtagtca agttccatct tctttcatcc
     2521 gtggagttgc ccgcatcaag tgcgggcctg gtgagaaagg ggagggacaa gcttagcgcg
     2581 cgaattcctt gaggagaatt tgcgccgtct catccatgtc cttccattcc gccagccttt
     2641 gacgcgagaa gcgcaccctt gccatgacgc cttggctgaa gggccagagg cgttcacaaa
     2701 taatcggacc cagcacggac ggcgtcttat cgcaccttat cacttcgccc aacggggcga
     2761 cgagaagctc ggaatcctta tagggtgaat cagccttgaa cctcctgagc ttgaggcctg
     2821 cttcctcact ttcgtccggg ctcagataaa cgctgtagat cggcgccatg cgctcttcaa
     2881 gcccggtgcg tccgaggcgt ggctcaagcg tgaccagcac ccagttgtgc aggtccgtcg
     2941 gcagggcgtg aacgatgttg agctgctggc ggtcaaaggg ccagggcagg cggaggtcaa
     3001 tgcgcgtcgc attgccgagg atcggcttgt caattttggc aacgatggtt tcaggcaggg
     3061 tgaatttggt gtcctccagg gtcgccacga caactgaggt cgacggtgcc cctatttccg
     3121 ggcggccaaa aagcagggac ggattgggca cgaagtagat cgccagaacg accccggcga
     3181 ggctgaagaa cgtcatcagc aggaacagga ggacaaatcc cagggagcgt tcgcgccggc
     3241 gttgcagaag atagttaaga tgatggctga ccataaggca aaactatagg tcgggccttt
     3301 cctctggtcc atgacttgct gcgtgtccca tgcgggaagt gtggatgtgc ctgaatgaaa
     3361 caggcgaggg gacataagca tggacatggt ttatttggcc tatctgtttg ccgttgccgt
     3421 cggcatcgta acggcgggta tcgcagcgag cctgtggacg tgggcggcgg gggaagaacc
     3481 gaggtttgca ctcctgcttg agccaagtgc cgtggcgccg cttcgtgccc tggtaatcgt
     3541 cgtttccgtg ccgctgctgc tcttgcttgc ggcctggcgc tatgtcggca gcgccagcgg
     3601 ggccatgctg ctggtggctg caagtttggg ctggagcttt ctgcaaggtg tatttatcct
     3661 gacgcggatc tttggcgtta cctgaacggg accttcccat ggcacttcat attcttgacg
     3721 ggatcgcgcc ggaactgccg gcggagggaa gctgctggat tgccgccagc gccgatgtta
     3781 tcgggcgggt gcggctggag cgaaacgcca gcatctggtt caatgcggtt ctgcgcggcg
     3841 ataacgagct gatctcggtg ggggagggca gcaatgtgca ggacggctgc gtgctgcata
     3901 cggacatggg ttcgccgctc accgtcggca gaaactgtac catcggccat aaggtcatgc
     3961 ttcatggctg cacgattggc gataacaccc tggtgggaat cggctcgatc attctcaaca
     4021 atgcggttat cggcaagaat tgcctgatcg gggcgcattc cctgatttcc gagcgcaagg
     4081 tcattcccga taattcactg gtgatggggt cgcccggcaa agtggtgcgg gaactcgatg
     4141 ctgaggcaat cgcccggctg agcgcggctg ccgcaagcta tgtgcggaat tggcagcgtt
     4201 atgccaaggg cctgcggccc gtttaggatt ttgctttccg cgcttttctg gccttgctgg
     4261 gaacctgggc tatggtctgg tccgtatcgg tgagcaagct caaggcagcc ttgcgggcgg
     4321 cattcgcatt tccacgcgcc acggctttca gaatgccttc gtgcttcgga atgaacatgt
     4381 cgggctcggc attgtgggtg gaggtgaatt caaacatgtt ggtaagggct gcctcaatca
     4441 tgatcccgaa tggcgagagc agcacattgt tggcggctgc cagaagcgcc aggtggaacc
     4501 tcacatcggg aaccaccgag ctgcggcccg gcctggcctt gcgcatatcc tcgaaggcct
     4561 cggtaatggc acgcagttgc tgcggtgtgc ggttaagggc ggcaagggcc gcgatctccg
     4621 gctcaaggat tttccgcact tcctgcaccg aagcgaagaa ttcctgcggg ttcatggagg
     4681 ccatgtgcca ggccagaaca tcgcggtcga ggatgttcca gttttcgcgg ggttcgacgc
     4741 gcgagccgat ctttgtccgg gacttaagca ggccttttgc cgtgagcgtc tttacggcct
     4801 cgcgcacggt ggtgcggctt gcgccataga tgcggcacca gtcggcctcg ttcggcaaaa
     4861 tcgcgccagg cgcatacacg ccactaagta tctgatttcc aatactttgc gccagcttcc
     4921 ggtgcaggtt actgccagcg gatctgcctg aagtgacttt tgggctcact tttccgggat
     4981 tcatttttat ctcgcttcat gccgaattgt catacaaata aggattgcgt cgctcaatcc
     5041 attatcctaa caatagcgtt tctgggtgaa actgcccgga aatctatacc gcagcaaggc
     5101 tgcttaatgg gaggaaaata tgtttactcg tagaaatttc ttggtcacct cggtggccgc
     5161 aggatcgctt ttggcaactg gccaggcgat ggcccagggc aagggcctta tcggcatttc
     5221 gatgccaacc aaatcgtcga cgcgctggat ttccgatggc gaatccatgg tgaaggaatt
     5281 caccgctctt ggctatgaca gcgatctcca gtacggcgaa gacgacattc cgaaccagct
     5341 tgcccagatc gaaaacatgg tcaccaaggg cgccaaggtt ctgatcatcg ccgcaatcga
     5401 cggttcaacc ctctcggaca tcctcaggaa agcccaggag gccggtgcga aagtgttcgc
     5461 ctatgaccgc ctgatcacca agaccgcgga tgtggactat tacaccacct tcgacaattt
     5521 cggcgtcggc gttctgcagg gcaattcgct gatcaagggt ctcaaggaac gctttgccgg
     5581 cgtgaagcca tggaacgtgg agctgttcgg cggttcgccg gacgacacca acgccttcta
     5641 tttctatgat ggcgccatgt cggtgctgca gccgctgatt gacgcgggcg acatcgccat
     5701 cgtttccggc cagatgggca tggacaaggt gggcacgctg cgttgggacg gtgcggtggc
     5761 ccaggcccgc atggataacc tgctgtcggc caattacacc gacaagaagg tgcatggcgt
     5821 gctggctccc tacgacggcc tgtcacgcgg catcatctcg tcgctcaagg gtgtaggcta
     5881 cgcaccgggc cccgaaatgc cgatcgtcac cggtcaggat gccgaagttc cgtcggtcaa
     5941 ggcgatgatg gccggcgagc agtattcgac agtgttcaag gacacgcgcg aactcgccgc
     6001 tgtgactgcc aagatggtgg acacggtcct gcagggcaag gagcctgaga tcaatgacac
     6061 caagacctac aacaacgaag tgaaagtcgt gccgtcctac ctcctgacgc cccacgacgt
     6121 gggcaaggac gacattgaaa aactgcttgt aggctcgggc tacatcaagg ccgaagacct
     6181 gcaataacgg attaatgatc ggcaggcccc gtgagctgac ttgcggggcc tgctgctttt
     6241 cgcgtatcaa cgcattcaag agcagggcaa acctgccgga cggtaaaggg aggtgggatc
     6301 ggccatgcag cctatccttg aaatgcgaga catcaccaag acgtttccgg gcgtgaaagc
     6361 cctgacggac gtcaaccttt ccgtgctgcc tgccgagatc catgccgtgg tcggcgagaa
     6421 cggcgcgggc aaatcgacgc tgatgaaggt tctctccggc gtctatcctg ctggaaccta
     6481 tgacggcgcc attcatttcg atggcgtgga acgcaaattc ggcaccattg ccgattccga
     6541 gaattccggc atcatcatca tccaccagga acttgcgctg gtgccgcttc tgtctattgc
     6601 ggaaaatatc tttcttggcc acgagaccgc gacgaacggc atcatcaact ggttcgagac
     6661 ttttgcccgc accaaggagc tgctgaagaa agtcggcctc aatgaacatc ccaacacgct
     6721 gatcaccaat atcggaaccg gcaagcagca gctggtggag attgctaagg cgctgaacaa
     6781 gaaggtcaag ctgcttattc tcgatgagcc cacctcgagc ctgaatgaaa cagattcaaa
     6841 cgcgctgctg gcgctgctca aggaattcaa ggcgcagggc atttcttcga ttctgataac
     6901 ccacaagctg aacgaggtgt tgaaggtcgc agaccgcatc accgtgctgc gcgacggcat
     6961 gacggtcgat acgctagaca cccacacggc caaggtgagc gaggaccgca tcatcaaggc
     7021 catggtcggg cgcgaactga cggaccgttt cccgccgcgc gcggcgaaaa taggcgaacc
     7081 cattttcgag gtcaggaact ggagtgtcta tcacccgctt catgcggaaa agcagatgat
     7141 caagggcgtg gatctcatcg cccgcaaggg cgaggttgtc ggcatagcgg gcctcatggg
     7201 atcagggcgg acggaatttg ccatgtcggt ctttggcggt gcctatggcc agaaaatcac
     7261 cggcgaagtc ctgattcatg gcaaacccgc taatgtcagc accattccgc gcgccatggc
     7321 cgcaggcctt gcctatgtca cggaggaccg gaagacctat ggcctggtgc tgatcgacca
     7381 tatcaagcac aatgtgaccc tggcaaatct cgagggcgta tcatcttacg cggtggtgga
     7441 cgagtcggct gaactcaaag tggccaacaa ataccggaaa gagctcggaa tccgttcttc
     7501 cggcgtgttc cagaccacgg acggcgccgt cgtcatggtc ggcgccttca agnnnnnnnn
     7561 nnacctttcg ggcggcaatc agcagaaggt ggtactgagc aaatggctgt tctccgggcc
     7621 tgatgtgctg atcctcgacg agcccacgcg cggcattgat gtgggcgcga aatacgaaat
     7681 ctacggaatc atcaaccggc tggcggaaga gggcaaggcg attatcgtca tctcctcgga
     7741 aatgcaggaa ctgctcggca tttcggaccg catttatgtg atgaacgaag gacgcttcgt
     7801 ggccgaaatg ccggccaaag aagccacgca ggaaaaaatc atgcgggcga tcatgaaatc
     7861 ttgggaaggc tgacatgact gacactgccg tacactcgca aacccggaac gacatgctgc
     7921 gcttcgtgaa aagcaacatc cgcgactacg ccctgctgct ttcgctgctg gtcatcatga
     7981 tattcttcca ggtcacgacg gacggcacgc tgttcaagcc ggtgaacatg accaatctca
     8041 tcctgcagaa cagctatatc gtcatcatgg cgctgggcat gctgctcatc atcgtggcgg
     8101 gccacatcga cctttcggtg ggctcggttt cgggctttgt gggggcggtg gctgccgtga
     8161 tgatggtgcc gtggaagatg gatccctttg tcaccatgct ggcctgcctt gcgctcggtg
     8221 ccgccatcgg cggggcgcag ggctattttg tcgcctacca ccggatcccc gccttcatcg
     8281 tcaccctggc gggcatgctg atcttcaagg gcctggcgct aaccgtgctg ggcggcgctt
     8341 cggtcgggcc tttcccccgg gaattccagc ttttgtcatc aggctatgtg cctgacgtgt
     8401 tcaacatcac tttcatgggc gggcccttca acctgctggc cctgatcatc ggggccgtgg
     8461 ttaccgccac gatcatctat ttcaacatca aggagcgccg cgagcagcag gcccatggcc
     8521 tggcggaaga gccgcgcatc atctacctc
//
LOCUS       DAORVN010000006         8484 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_4730_length_8484_cov_3.543006, whole genome shotgun sequence.
ACCESSION   DAORVN010000006 DAORVN010000000
VERSION     DAORVN010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 8484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8484
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4730_length_8484_cov_3.543006"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..497
                     /locus_tag="PKV23_00285"
     CDS             <1..497
                     /locus_tag="PKV23_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019195249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HQX83896.1"
                     /translation="AVTEGVWPPKGTAIFDPWHLPLLNTLILLTSGTTVTWAHHALLN
                     NDRQGLKWGLIATVALGALFTAVQAYEYAHAAFAFSRDNGGNIYGSTFFMATGFHGFH
                     VLIGTIFLLVCLFRALKGHFTPQQHFGFEAAAWYWHFVDVVWLFLFACIYVWASSGVA
                     ILHE"
     gene            511..885
                     /locus_tag="PKV23_00290"
     CDS             511..885
                     /locus_tag="PKV23_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010658403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF983 domain-containing protein"
                     /protein_id="HQX83897.1"
                     /translation="MDENYYPDLSPLTTGLAGKCPRCGRGKLFDGYLSVAKSCRSCGL
                     SYAFADSGDGAAWFVMLFVCVAGVGSILGVEIAYAPAFWVHILLAIPILIIMPLVLLR
                     PVKGVLLCQQWKTKAREGRTDP"
     gene            882..1601
                     /locus_tag="PKV23_00295"
     CDS             882..1601
                     /locus_tag="PKV23_00295"
                     /inference="COORDINATES: protein motif:HMM:NF014193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HQX83898.1"
                     /translation="MKPRIWPIVMWAGLGITVLLALGTWQLFRLAEKEAQLAEFAERA
                     KAPPLSLTEVLARRGEGTDIEFDAVKVRGVFDHAAERHKLTSFEGSPGFQIITPFRSD
                     DGIMVLVDRGVVSAGLKDAAKRNESPGPVELSAVVRAHGKERGFFDPDNDVDGNLWYW
                     WDVPAMLSSVAIAPDLQVAPFVLQELPGSDPNRFPRPAPPEVQFTNNHLQYALTWFAF
                     AATLLVIAGLFIRKLRTTTDA"
     gene            1652..3043
                     /gene="thrC"
                     /locus_tag="PKV23_00300"
     CDS             1652..3043
                     /gene="thrC"
                     /locus_tag="PKV23_00300"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007874044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HQX83899.1"
                     /translation="MQYVSTRGEAPELGFEDVLLTGLARDGGLYVPKSWPQISADELR
                     DLRGRSYQDVAVHVVSKFTGSAIPQSDLMQMIAEAYATFGHPAVTPLKQMDANQWLLE
                     LFHGPTLAFKDVAMQLLARLMDWSLKKRASRVIIVGATSGDTGSAAIDAFKGSRNATT
                     VILHPHGRVSDVQRRQMTTVMSPAIHNIAIEGNFDDCQAIVKALFNDLKFRDSVNLAG
                     VNSINWARIMAQVVYYVSSAVALGAPDRRVSFCVPTGNFGDIFAGYTAKRMGLPIDRL
                     IIATNLNDILDRTLKTGRYEMRGVKPSSSPSMDIQVSSNFERLLHEAYGRDAAAIRGL
                     MAGLSQSGAFSIGQRELAAIRGEFDSFAANEAETAETIARTLKATGELLDPHSATGFA
                     AALRAKASNSAMITLATAHPAKFPDAVEKASGVRPHLPQRLSHLLNAKERFTMLPNSA
                     TAVREFILALDKA"
     gene            3058..4323
                     /locus_tag="PKV23_00305"
     CDS             3058..4323
                     /locus_tag="PKV23_00305"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HQX83900.1"
                     /translation="MGVEITCLPNGLNVLTHAMPHIETVALGIWVKAGARDERPEENG
                     IAHFLEHMAFKGTKRRTAKDIAEEIESAGGEINASTGMETTTYYARVLKDDWALALDI
                     LADIFTESALDDEELERERDVILQEIAAANDQPDDLVFDLAQNASYGDHPLGRSILGT
                     AELIAAVDREKMLAWRDRNYWASRIVVAAAGNIDHAKFAEAAAKLFGSIERGQQPQRE
                     PPSFSGTEQKAQKPLDQTHVVLSFAAPSYRDPEIYQLQVLSSILGGGMSSRLFQEVRE
                     KRGLCYSVFAFGSAYEDAGQIGVYAASSPEHTEELLDVTSSVMMSVTSAVTDAEVARA
                     KAQLKTSLVMNLESASSRADQIARQFLAFGLVPAISTLVEKIEAVTPDQVRDLAARLF
                     QDKVPAVSAVGQLSHLSAHKKISGYFSRH"
     gene            4336..4917
                     /locus_tag="PKV23_00310"
     CDS             4336..4917
                     /locus_tag="PKV23_00310"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HQX83901.1"
                     /translation="MVFLKSPFASEPMPQLHQGPVVLRPPVLSDFESWVRLRQESKSF
                     LAPWEPLAANYEFSKFNFRSRIKHYQLQAKNDAAYAFFIFHREQEHLLGAITASNVRR
                     GAAQMCSVGYWIGKPHARQGYMGHALNALVGHAFNGLSLHRVEAACLPSNQASIALLR
                     RCGFSEEGMARKYLNIAGKWQDHLLFAKLQDGS"
     gene            4995..8237
                     /locus_tag="PKV23_00315"
     CDS             4995..8237
                     /locus_tag="PKV23_00315"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HQX83902.1"
                     /translation="MLLRFASFLLLIVLGLANPALAQSRLDVGAMGSSIDLAPYLQQL
                     TTEKPEVMVELPRDATGKVGVIALKSSRSVPSYHWRILTLVNTGKIPKNLIVVIDHQK
                     FAGSRLLYPLNPGSVVASIAHTGSVAPARISALGQDAYSLVLEPSANLSLGLEMTGTA
                     PSALLWERDAFDSLSRSIAFFRGAILGVAVLLSVSMLLLYTTRARASFLSGGILALAS
                     VAFVALEAGYFAQARKLFLGFAVSPAEARAVIESLMAVGLLLCLTALADLRRTVPLLR
                     NVFVGLALAGAALPVYAFVDPLLVASIARIAFAATAIIGFVILFRFRQIRPAESALLL
                     WSTVVIWTFMAAMAAVSKGGSTYVSSALLLGLIVVLVFLTFRFAQSAASTGLLSRRLF
                     QEAGRRGLALAGAQQYVWDWQPEDQNLFVSEELEQALGHPPGTLTEASAESLLDLMHP
                     ADRVAYLATVENAERRGRGLINQELRLRRADGTYRWFELRARAMADSDNRMVRSIGTL
                     SDITNAKRTEERLLNDAVYDRVTGLPNRALFVDRLKREIAKPNSENLHVVLVDIDRFK
                     TVNDGLGHDAGDSLLMVLGRRIEQLLGPEDTVARLPGDQFAVLFSDSGQTRDIRRFTD
                     SLRQTIAKPVNVNQQEIFMTACLGVASTREGGSTAEQLMKDAAIALYEAKRRGKEMIE
                     FFRSSMRDERGELVALEAELRRALERNEIEVHYQPIARLSDMDLAGFEALVRWRHPSL
                     GLLAPESFLDLAEQTGMIKDIGRFVLNEATRQLGIWQRAFRPSEPVFMAVNVSASQLM
                     DTDLVADIRAAVTRETVYRQTLKIEVTESAVMQYPEKSASLLELVKQLGVGLACDDFG
                     TGYSSLSSLRHLPFDTLKVDRSFVSPEAEDEKAAIILQSIVSLAHDLGLTIVAEGIED
                     QAQVDRLVELECDYGQGFFIGQPMTAKQVGESLTSMPYAKGHGRTAMSNLWERATHEP
                     VPLPVETELTVDAIESAQQPVVNPGPPQSDEWKAIKAVALAIQARKPVAISPPPGSSP
                     MAPRLVRAEPEELPGIPLSVPKPAKKPKRKTPGKKRQASKKETPEASGVA"
     gene            complement(8223..>8484)
                     /locus_tag="PKV23_00320"
     CDS             complement(8223..>8484)
                     /locus_tag="PKV23_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012564456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HQX83903.1"
                     /translation="SVALTATVEILQAIARGNGPGKVMLALGYSGWVAGQLESEIQHN
                     GWLHCEADDDLIFGTDNDGKYARALRKIGVDPLMLSSDSGHA"
BASE COUNT         1570 a         2798 c         2428 g         1688 t
ORIGIN      
        1 tggcggtgac cgaaggtgtc tggccgccaa agggcacggc gatcttcgat ccctggcatc
       61 tgccgctgct caacaccctt atccttctca cctctggcac gacggtgacc tgggcccatc
      121 acgccctcct caacaatgac cgccagggcc tgaaatgggg cctcatcgca accgtggctt
      181 tgggcgcact tttcaccgcc gtccaggcct atgaatatgc ccatgcagcc ttcgccttca
      241 gccgcgacaa cggcggcaac atctatggtt ccaccttctt catggccacc ggcttccatg
      301 gcttccatgt gctcatcgga accatcttcc tgctcgtctg cctgttccgc gccctgaaag
      361 gccacttcac cccgcagcag catttcggct tcgaggccgc tgcctggtat tggcatttcg
      421 tggacgtggt ctggctgttc ctctttgcct gcatctatgt ctgggcttcg tccggcgtgg
      481 cgatcctgca cgagtaatcg cttttgcagg atggatgaga attattatcc cgacctttct
      541 ccgctcacca cggggcttgc tggaaaatgc ccccgttgcg ggcggggaaa actgtttgac
      601 ggctatctga gcgtcgcaaa atcttgccgg agctgcggcc tctcctacgc ctttgccgac
      661 agcggcgacg gcgccgcctg gttcgtcatg ctcttcgtct gcgtcgcagg cgtaggctcg
      721 atcctgggcg tcgagatcgc ctacgcgcct gccttctggg tccatatcct gctggccata
      781 cccatcctca tcatcatgcc gcttgtcctc ttgcgcccgg tgaagggcgt cctgctctgc
      841 cagcaatgga aaaccaaggc ccgcgaaggc cggaccgacc catgaaaccg cgtatctggc
      901 caattgtaat gtgggcgggg ctgggcataa ccgtgctcct ggcactcggc acctggcagc
      961 tctttcgcct tgctgaaaaa gaagcgcagc tcgccgaatt cgccgaacgc gccaaggccc
     1021 cgccgttgtc cctcacggaa gttctcgccc gccgcggcga gggcaccgac atcgaattcg
     1081 atgcagtaaa agtgcgggga gtgtttgatc atgcggctga gcggcacaag ctcacctcat
     1141 ttgagggaag ccccggcttt cagatcatca cgccgttccg gagtgatgat ggcatcatgg
     1201 tcctggttga ccgcggggtt gtatccgctg gcctgaagga cgccgccaaa cggaacgaaa
     1261 gtcccggacc cgtggaattg agtgccgtgg tgcgcgccca tggcaaggag cgtggttttt
     1321 ttgatcccga caatgatgtg gatggcaatc tctggtactg gtgggatgtg ccggccatgc
     1381 tgtcctccgt agccattgcc cctgacctgc aagtggcccc gttcgtcctc caggaacttc
     1441 ccggcagtga tcccaaccgc tttccacgcc cggcgcctcc ggaagtgcaa ttcaccaaca
     1501 atcacctgca atacgcgctc acctggttcg ccttcgccgc aacactcctt gtgatcgccg
     1561 gcctcttcat ccgcaaattg cggacaacaa ccgatgcttg aagccatacg gcgtccccgc
     1621 tagtttgcaa tcccgattga aaggttcctc cgtgcaatat gtgtccacca ggggcgaggc
     1681 cccggaactt ggctttgaag atgtcctcct caccggcctt gcccgtgatg gcggcctcta
     1741 tgtgccgaag tcatggccgc agatctctgc cgacgaactg cgggacttgc gcgggcgctc
     1801 ctatcaggac gtggccgttc acgtagtttc gaaattcacc ggcagcgcca ttccccaatc
     1861 cgacctgatg cagatgatcg ccgaagccta cgccaccttt ggccatcccg cggtaacacc
     1921 cctgaagcag atggacgcca atcaatggct gctcgaactt ttccacgggc ctacgctcgc
     1981 cttcaaggat gtggccatgc agttgctggc ccgcctgatg gactggtcgc tgaagaagcg
     2041 cgccagccgc gtcatcatcg tcggcgccac ctcgggggat acgggcagtg ctgccatcga
     2101 cgccttcaag ggcagccgga acgccaccac ggtcattctt catccccatg gccgggtgag
     2161 cgatgtccag cgccgccaga tgaccacggt gatgagcccc gccattcaca acatcgccat
     2221 cgaaggcaat ttcgatgatt gccaggccat tgtgaaagcg ctgttcaacg atctgaaatt
     2281 ccgcgacagc gtgaacctag cgggcgtcaa ttccatcaac tgggcccgca tcatggccca
     2341 ggtggtttac tacgtctcat ccgccgtggc gctgggcgcg ccggaccgca gggtgagctt
     2401 ctgtgttccc accggcaatt tcggcgacat tttcgccggc tacaccgcca agcgcatggg
     2461 ccttcccatc gaccgcctga tcatcgccac caacctgaac gacattctcg accgcaccct
     2521 gaaaaccggc cgctatgaaa tgcggggcgt gaaaccaagc tcgagcccct ccatggatat
     2581 ccaggtgtcg agcaatttcg aacgcctgct gcatgaagcc tatggccgcg atgccgccgc
     2641 catccgcggc ctgatggcgg gcctctcgca atccggcgcc ttcagcattg gccagaggga
     2701 actggcggcc atccgcggcg aatttgactc ctttgccgcc aatgaggccg aaaccgccga
     2761 aaccattgcc cggaccctca aggcaaccgg cgagcttctg gacccccaca gcgccaccgg
     2821 ttttgccgcc gcgctgcgcg ccaaagccag taattctgcg atgattaccc ttgccaccgc
     2881 gcaccccgca aaattccccg atgcggtcga aaaggccagc ggtgtaaggc cccatttacc
     2941 ccaaagacta tcccacttgc tcaatgccaa ggaacgtttc actatgcttc caaacagcgc
     3001 cacagcggtc agggagttca tcctggccct ggataaggcc tgaattggga tcgcgttatg
     3061 ggtgttgaga ttacctgtct gccaaatggg ttgaacgtgc tgacccatgc catgccccac
     3121 atcgaaaccg ttgccctggg gatctgggtg aaagcaggcg ccagggacga aaggccggaa
     3181 gaaaacggca tcgcccattt cctcgaacac atggccttca agggcaccaa gcgccgcacc
     3241 gccaaggaca tcgccgagga aattgaatcg gcgggcggcg aaatcaacgc ctcgaccggc
     3301 atggaaacca ccacctatta tgcccgcgtg ctgaaggacg actgggccct ggcgcttgat
     3361 atcctggccg acatcttcac cgagtccgcc cttgacgatg aggaactgga acgcgagcgc
     3421 gatgtcatcc tgcaggagat cgccgccgcc aatgaccagc ccgacgacct ggtttttgat
     3481 ctcgcccaga acgccagtta cggcgaccat cccctgggcc gttcgatcct cggcacagcg
     3541 gaactgatcg ccgccgtgga ccgcgaaaag atgctggcct ggcgcgaccg gaactattgg
     3601 gcctcgcgca ttgtcgtggc cgccgccggc aacatcgacc atgcaaaatt cgccgaagcg
     3661 gcggcaaaac tctttggcag catcgagcgc ggccagcagc cgcagcgcga gccgccgagt
     3721 ttttccggca ccgagcagaa ggcgcaaaag cccctcgacc aaactcatgt agtgctttca
     3781 tttgccgcgc caagctaccg tgatcccgag atttatcagc ttcaggtgct gtccagcatt
     3841 ctgggcggcg gcatgtcatc aaggcttttc caggaggtcc gcgagaagcg cgggctgtgc
     3901 tacagcgtct tcgcctttgg ctcggcctat gaggatgcgg gccagatcgg cgtctatgcc
     3961 gcaagctcgc ccgaacacac cgaagaactg ctcgatgtga cctcgagcgt gatgatgtcg
     4021 gtaaccagcg cggtcaccga tgcggaagtg gcccgcgcca aggcccagtt gaaaaccagc
     4081 cttgtcatga atcttgaaag tgcgtcgtcg cgcgccgacc agatcgcccg gcagttcctg
     4141 gccttcggcc tggtgcccgc catctccacg ctggtggaga aaattgaggc ggtgacgccc
     4201 gatcaggtcc gcgaccttgc cgccaggctg ttccaggaca aggtgcccgc cgtcagcgcc
     4261 gttggccagc tgtcccatct ttcggcccac aagaaaattt ccggctattt ttccaggcac
     4321 tgacggaagc ggcagatggt ctttctgaaa tcaccctttg ccagtgaacc gatgccgcaa
     4381 ctgcatcagg gcccggttgt cctgcgtccg ccggttttgt ccgatttcga aagctgggtg
     4441 cgcctgcgcc aggagagcaa gagcttcctc gccccctggg agcccctggc ggcaaactac
     4501 gaattcagca agttcaattt ccgctcgcgc atcaagcatt atcagttgca ggcgaaaaac
     4561 gatgcggcct atgccttttt catattccac cgcgagcagg agcatcttct cggcgccatc
     4621 accgcttcga atgtgcgccg tggcgccgcc cagatgtgtt ctgtcggcta ctggatcggc
     4681 aagccccatg cccgccaggg ctacatgggc catgcgctga acgcccttgt cggccatgcc
     4741 ttcaatggcc tcagcctgca ccgcgtcgaa gctgcctgcc taccctcgaa ccaggcctcc
     4801 atcgcgctgc tgcgccgctg cggcttttcc gaggaaggca tggccaggaa atatttgaac
     4861 atcgcgggaa aatggcagga ccacctgctg ttcgcgaagc ttcaggatgg cagttagcgg
     4921 cagttcttgc cagtcgggtc gcggcacgtt acgtagtacc ccgccagttt aaccggcaca
     4981 actaggacgg acggatgtta ttgcgctttg cttcgtttct gcttctgatc gttctcggtt
     5041 tggcaaatcc ggcgttagcc cagtcgcgcc tcgacgtggg cgccatgggc tcaagcatcg
     5101 atcttgcacc gtatttgcag cagcttacta ccgaaaagcc tgaggtcatg gtggaactgc
     5161 cccgtgatgc tacggggaaa gttggcgtca ttgcgctcaa gtcctcccgt tcggtgccgt
     5221 cctaccactg gcggatactg acgctggtaa acaccggcaa gattccaaaa aacctgatcg
     5281 ttgtcatcga tcaccagaaa ttcgccggct cgcgcctgct ctatcccctg aacccgggaa
     5341 gcgtggttgc cagcatcgcc catacgggaa gcgttgctcc cgcccggatt tccgccctgg
     5401 gacaggatgc ttacagcctt gtgctcgaac cctcggcaaa cctgagtctc gggctggaaa
     5461 tgaccgggac agctcccagc gccttgctgt gggagcgcga cgcgtttgat tcgctgtcgc
     5521 gttccatcgc cttcttccgc ggcgccatcc tgggtgtagc cgtattgctg tcggtgagca
     5581 tgctgctgct ctacaccaca cgcgcccgcg cgtcgtttct ctctggcggc atactggcgc
     5641 ttgcctccgt ggcttttgtg gccctcgagg ccggctattt tgcccaggcg agaaagctct
     5701 tcctcggatt tgcggtttcg cccgccgaag cccgcgccgt catcgagtcg ctgatggccg
     5761 tgggcctcct gctgtgcctg acggccctgg cagatctccg gcggaccgtt cccctcctgc
     5821 gcaatgtttt tgtgggactg gcgctggcgg gtgcagcact cccggtttac gctttcgtcg
     5881 atcccctgct tgtggcgtca atcgcccgca tcgcctttgc cgccacggcc atcatcggtt
     5941 ttgtcatcct gtttcgcttt cgccagatcc ggccggcgga atcggccctg ctgttgtggt
     6001 ccaccgtcgt catctggacc ttcatggcgg cgatggccgc cgtttcgaaa ggcggcagca
     6061 cctatgtttc atcggccctg ctgctcggcc tcatcgtcgt tctcgttttc ctgacctttc
     6121 gcttcgccca aagcgccgcc agcaccggcc ttctctccag gcgcctgttc caggaggcgg
     6181 gccgccgcgg cctggcgctc gccggcgccc agcaatatgt ctgggactgg cagcccgaag
     6241 atcaaaacct gttcgtgagc gaagaactgg aacaggccct cggccatccg ccgggaaccc
     6301 tgaccgaggc aagcgccgag tccctgcttg atctcatgca tccggccgac cgtgtcgcct
     6361 atctcgccac cgtggaaaat gccgaacgcc gcggccgcgg cctcatcaac caggaactgc
     6421 gcctgcgccg ggccgacggc acctaccgct ggtttgagct cagggcccgc gccatggccg
     6481 attccgacaa ccgcatggtg cgcagcatcg gcaccctttc cgacatcacc aatgcgaagc
     6541 ggaccgagga acggcttctc aacgacgcgg tttacgaccg cgttaccggg cttcccaacc
     6601 gcgccctgtt cgtcgaccgg ctgaagcgcg aaatcgccaa gccgaattcc gaaaacctgc
     6661 atgtcgttct ggtcgacatc gatcgcttca agacggtgaa cgatggcctc gggcatgatg
     6721 ccggcgacag cctgctgatg gttcttggcc gccgcattga gcagctcctt ggtccggaag
     6781 acacggtggc gcgcctgccc ggcgaccagt tcgccgtcct cttctccgat tccgggcaga
     6841 cgcgcgatat caggcgcttc accgacagcc tgcgccagac gattgccaag ccggtgaatg
     6901 tcaaccagca ggaaattttc atgaccgcct gcctgggcgt tgccagcacc cgtgagggcg
     6961 gctcgactgc cgagcagctc atgaaggacg ccgccatagc cctttatgaa gccaagcgcc
     7021 gcggcaagga gatgatcgag ttcttccgtt catccatgcg cgacgagcgc ggcgaactcg
     7081 tggcccttga agcggaattg cgccgcgccc tggagcgcaa tgaaatcgaa gtgcactatc
     7141 agccgatcgc acggttaagc gacatggacc tggcgggctt cgaggccctg gtgcgctggc
     7201 gtcatcccag cctcgggctt ctggcgccgg aaagcttcct cgaccttgcc gaacagaccg
     7261 gcatgatcaa ggacatcggc cgcttcgtcc tgaacgaagc aacccgccag cttggcatct
     7321 ggcagcgcgc cttccgtccc tccgaaccgg tcttcatggc ggtcaacgtc tcggcaagcc
     7381 agctcatgga cacggatctg gtggccgaca tccgcgccgc cgtcacccgc gaaaccgttt
     7441 accgccagac cctgaaaata gaagtcaccg aatccgccgt gatgcagtat ccggaaaaat
     7501 ccgccagcct gctggaactg gtcaagcagc tgggcgtggg gctggcctgc gatgatttcg
     7561 gcaccggtta ctcctcgcta tcaagccttc gccatttgcc ctttgatacg ctgaaggtgg
     7621 accgttcatt tgtgtcgccg gaagccgagg atgaaaaagc cgccatcatc ctgcaatcca
     7681 tcgtttcgct ggcccatgac cttggcctga cgattgtcgc cgagggcatt gaagaccagg
     7741 cccaggttga ccggctggtg gagctcgaat gcgattatgg ccagggattt ttcatcggcc
     7801 agcccatgac cgccaaacag gtgggcgagt cgctgacctc gatgccttac gccaagggcc
     7861 acggccgcac cgccatgtcg aacctctggg aacgcgccac ccatgagccg gtgccgcttc
     7921 cggtggagac cgaactcacg gtggatgcca tcgagagcgc ccagcagccc gtggtgaatc
     7981 ccgggccgcc gcaaagtgac gagtggaagg cgatcaaggc cgtggccttg gccatccagg
     8041 cgcgcaagcc cgtggcgatt tcccctccgc ccggctcttc gcccatggcg ccgcgcctcg
     8101 tcagggccga gcccgaggaa ctgccgggca ttcccctgag cgtgcccaag ccggcgaaaa
     8161 aacccaaaag gaaaacgccc gggaaaaagc ggcaggcttc caaaaaggaa actcccgaag
     8221 cctcaggcgt ggcctgaatc agacgagagc atcagcggat cgacgccgat tttcctgagg
     8281 gcgcgcgcgt attttccgtc attgtcggtg ccgaagatga ggtcgtcatc ggcctcgcaa
     8341 tggagccagc cattgtgctg gatttcactt tcgagctggc ccgcgaccca gcctgaatag
     8401 cccagcgcca gcatgacttt ccccgggccg ttgccccggg cgatcgcctg gaggatttca
     8461 accgttgcgg tcagcgccac gctg
//
LOCUS       DAORVN010000007         7673 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_5575_length_7673_cov_2.624180, whole genome shotgun sequence.
ACCESSION   DAORVN010000007 DAORVN010000000
VERSION     DAORVN010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 7673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7673
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5575_length_7673_cov_2.624180"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(268..1089)
                     /locus_tag="PKV23_00325"
     CDS             complement(268..1089)
                     /locus_tag="PKV23_00325"
                     /inference="COORDINATES: protein motif:HMM:NF016974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl/asparaginyl beta-hydroxylase
                     domain-containing protein"
                     /protein_id="HQX83904.1"
                     /translation="MSKIKDSTGQTWLQRFRKARRKKVKIWGRSLTRSLASFLGRQSL
                     VGDTPVLDSRHFPFLASFTGNWQAIRAEAIEILKHRDAIPAFHNISPDQKKISKGTNW
                     RTFILFGFGNKLEKNARQAPLTTKLLETVPDLQTAWFSILSPGYHIPAHRGVSKGIVR
                     AHLGLLIPREAEKCRMRVGDTINVWRPGEIFVFDDTYEHEVWNDTDEERVILLFDFDR
                     PMRFWGRLVNNTFLGLMKFTAFYQEPKKNLADYEDRFEAATRRANDNMEKLSDND"
     gene            1221..2300
                     /locus_tag="PKV23_00330"
     CDS             1221..2300
                     /locus_tag="PKV23_00330"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006206863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HQX83905.1"
                     /translation="MAGKRVIVVGAGIIGASIAWHLAKAGAEVTVIEAGQPGGVATRN
                     SWAWINASWGNPEIYFRLRTRSMAEWRRIDREVPGLEVSWCGGLTWDLPPDQLEAYAV
                     EHSAWGYGIKRVDRAGALRIEPNIKQPPDFALHVPEEGKVEPLAAALALLAGAEALGA
                     TVLRGSPVKWLEEKHGRVVGVCLNDGVLHADEVVIAAGAESGALLGSIGLSYDIGAPA
                     GLLVHSKPAGEILHTLLQTPEVHVRQTSEGRLVAGADFAGTDPADDPRGAATALYQKV
                     QDMVVGAEQVELDFHTVGFRPTPGDGFPMIGRPQDREGLYITVMHSGITLAPAVGLFA
                     TAELLDGKRDPLIAAYPPDRLLKAK"
     gene            2297..3370
                     /locus_tag="PKV23_00335"
     CDS             2297..3370
                     /locus_tag="PKV23_00335"
                     /inference="COORDINATES: protein motif:HMM:NF016999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HQX83906.1"
                     /translation="MNLHTVSSLSVLRALAIGTAGGALGHVIGIPAPWLAGSLVATII
                     AVYSNQTLELPDWLRTLAFILLGIQTGTAVNSETLYRAGQWPLSIVCMGVTVGLIVWA
                     CMVYYERLRKWNKATAYFSSLPGALSMVILLASTSKADMRRVTISQCVRLFFLIAALP
                     LVIVYISPAPPAFPATPAVASIFEIIILVGVSAAAGLLFERLRVPAGLILGAALASAA
                     LGLGDVIHGGAPDSILIPANIILGVMIALRFKGISWPELRISLGDGFAGFAIAMVIAV
                     AGALFTSQIAGLPLALTLLAFAPGGLEAMTIMAFALNLDPAYVAAHQVARYIGLVLLM
                     PAVTSFVLHRNVPAPQPVNVEED"
     gene            3492..4010
                     /locus_tag="PKV23_00340"
     CDS             3492..4010
                     /locus_tag="PKV23_00340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX83907.1"
                     /translation="MAEHDDLSGALRKARLAEAAHFEAVLGIKDSKSLRLQVLKDDMA
                     PLIAATPEAANVFDLALVPGEPPRLWIDLISFVEMEPDYRTYRLQQDNQGGREALFET
                     TDRAEMVTYLRSYLAHRMIARERQIVRAAPVSQPAARYTTGAVVYAWFNGLILGFLAL
                     VVAAISLGKLKF"
     gene            4157..5971
                     /locus_tag="PKV23_00345"
     CDS             4157..5971
                     /locus_tag="PKV23_00345"
                     /inference="COORDINATES: protein motif:HMM:NF006624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HQX83908.1"
                     /translation="MTAKAAKQEAVAVSAGLARTPRANRDLEQKLAGFTTIAEGLDYA
                     ACGLTGFNFYSAKGVLSDVLTYAELRRRALATARKLMSTGLKRGDRVAVIAETGPEFM
                     AVFFGCQYAGLVPCPMPYTMYIGGRDAYVERVTGMLRAAAACAVVTSSDLEGHILAGA
                     AGAGVEKVLTHEALQALPESLTKLEAFGPDDVAYIQYSSGSTSEPKGVLITQKAIMAN
                     THGILRDGLRVTKSDRAFSWLPLYHDMGLVGFCLAPMMGQVSVDYISTPSFARRPALW
                     LRLMSENRSTISYSPSFGYDLAARRINGEAVTLDLSNWRAAGIGGDMVRADVLKNFGD
                     TLSVAGFNHNAFMPSYGMAESTLAVSFSDVAQPLRIDTIDKHMFKLSGRAVPAPETAD
                     KDAVRSFVVCGKPLPGHQMVVRDEMGTVLRDREIGHIFVKGPSLMSGYYHNARATDAV
                     IAADGFLATGDMGYMLDGEIVITGRAKDLILHNGRNIWPQDIEWAAEQVEPLKSGDVA
                     AFAVEGDDGCDDVVVLVQCRVTTDAEIEKLRREVSAVVHHSAGVECAVVLVPPKSLPF
                     TSSGKLSRAGAKQKYVSGELVEITQRAFQAADFLAAAQ"
     gene            5990..6955
                     /locus_tag="PKV23_00350"
     CDS             5990..6955
                     /locus_tag="PKV23_00350"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HQX83909.1"
                     /translation="MPLKIALTGATGFVGRAVVSAFAAESCRLSALVREPARAGLNPS
                     VRQVEGDLQSLAALDDLTRGADAVIHIAGVIGALSRDEFFAANEQGSRAVAEAAARNG
                     VARFVHISSLAAREPELSLYGASKRAGEAAVEAFMDRMSVVILRPPAVYGPGDRGTLP
                     LLRSLTQSLAVIPGRSTSRFSLIYVDDLARIIVEAATAARAGVVELDDGRRQGYGWRD
                     LAHIAAASEQKSIKPIFLPKSVAMAVGMGAEAVARLRGKLPFVSPDKIQQLYFGDWVA
                     RGEGWPLKEPVDFARGFKSTVDWYRKAQWLPERPAARRIATQERS"
     gene            6952..7200
                     /locus_tag="PKV23_00355"
     CDS             6952..7200
                     /locus_tag="PKV23_00355"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HQX83910.1"
                     /translation="MTASQETIDQICKLLEPFNTTGKVLSPETEISGDLNIDSVAVMD
                     FVMEVEDHFDIEIPLNVVSETHSIGDLAAVVEARVKKA"
     gene            7205..>7673
                     /locus_tag="PKV23_00360"
     CDS             7205..>7673
                     /locus_tag="PKV23_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020186886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HQX83911.1"
                     /translation="MIDLLDKHKGIAERHARMMAMGVNAVGLTNDRILSPTRAMINGR
                     ETILAGTNNYMGITFDADCVKAGQDAMAEFGTGTTGSRIANGSYALHKELEAELAKFL
                     KRKHCIVFTTGYQANLGMMSGLAGPKDTIYLDADSHSSIYDGCIDEGINIIDTR"
BASE COUNT         1411 a         2189 c         2477 g         1596 t
ORIGIN      
        1 agctgcggcc cctcaaacgt gccgcccgca atgggcgcga cgcggcggct gccaaagggc
       61 gtaggaccca cgacatggtg ggcaccgaca tccagagcaa gacggaacag gaacttcgtg
      121 cggatctcgg tcataggctg ctcccagttt tactgtgtaa cgtggaatcg atggcgcgtg
      181 acggatgggg ttcggggttc caccttcccc agaccaaggg ctggagaagg atcaaagtga
      241 tgtaattcaa ccaaagccgg caagactcta atcgttgtcg gagagttttt ccatgttgtc
      301 gttggcgcgg cgggtggcgg cttcgaaacg gtcttcgtaa tccgcgaggt ttttcttggg
      361 ctcctggtaa aaggccgtga acttcatcag gccaaggaag gtgttgttga ccagcctgcc
      421 ccaaaagcgc atgggccggt caaaatcaaa gagcaggatg acgcgctctt catccgtgtc
      481 attccacact tcgtgctcgt aggtatcatc aaaaacaaag atttcgccgg ggcgccagac
      541 attgatggtg tcgcccaccc gcatgcggca tttctccgcc tccctaggga tgagcaagcc
      601 gagatgggcc cggacgattc ccttgctgac gccgcgatgg gcgggaatgt ggtagccggg
      661 actgagaatc gagaaccatg ccgtctgcag atccggcacg gtttcaagca gcttcgtcgt
      721 gagcggcgcc tgcctggcat tcttttcgag tttgttgccg aagccgaaca ggatgaaggt
      781 gcgccagttg gtgcctttgg aaatcttctt ctggtcaggt gaaatattgt gaaaggccgg
      841 aatggcatca cgatgcttga gaatctcgat ggcctcggcg cgaatggcct gccaattccc
      901 ggtgaaactg gcgaggaatg gaaagtgcct tgaatcaagc accggggtat cgcccaccag
      961 ggattgccga ccgagaaaac ttgcaagact gcgggtgagg gagcgccccc agatcttgac
     1021 cttcttgcgc cgggctttcc tgaaacgctg cagccaggtt tggcctgtgg aatctttgat
     1081 cttgctcatc aggaggagct atattcgatg ctttcactga actcaaccga ggcgggcagc
     1141 ttccggatca cggtcactgg ccagagcgcg aatttgcggc taacccagtc tcaatcaggg
     1201 aaatctgcgc gggagaatct atggccggca agcgtgtcat tgttgtagga gcgggcatca
     1261 tcggagcctc catcgcctgg catttggcca aggctggcgc cgaggtcacg gtgatcgagg
     1321 cgggccagcc cggcggcgtt gcgacgcgca actcatgggc ctggatcaat gccagttggg
     1381 gcaatccgga aatctatttt cgcctgcgga cccgctccat ggcggaatgg cggcgcattg
     1441 accgtgaggt gccggggctg gaggtgagct ggtgcggcgg gctcacctgg gacttgccgc
     1501 cggatcagct tgaagcctat gcggtcgagc atagcgcgtg gggctacggc atcaagcggg
     1561 ttgaccgggc gggggcgctc cgcattgaac ccaatatcaa gcagccgccg gattttgccc
     1621 tgcatgtgcc tgaggaaggc aaggttgagc cgctggccgc ggccctggca ttgctcgcgg
     1681 gcgctgaagc tttgggcgcc acggttttgc gcggcagccc ggtgaagtgg cttgaagaaa
     1741 agcatgggcg tgtggttggc gtctgcctca atgacggggt gctgcatgcg gatgaggtgg
     1801 tgattgcggc gggggccgaa tccggcgccc tgctgggcag cattggcctg tcctatgaca
     1861 tcggcgcacc ggcagggctt ctggttcact cgaagccggc aggcgaaatc ctgcatacgc
     1921 tgctgcagac gccggaggtg catgtgcggc agacgtccga ggggcggctt gtggccggag
     1981 ccgatttcgc gggaactgac cccgccgatg atccgcgcgg tgcggccacg gcgctctatc
     2041 agaaagtgca ggacatggtg gtgggcgcgg agcaggtgga actggatttt cacacggtgg
     2101 gattcaggcc aacgccaggg gatggtttcc cgatgattgg gcgtccgcag gaccgcgagg
     2161 gactttacat cacggtcatg cattcgggca tcacgctggc gcctgcggtt gggctttttg
     2221 caactgccga attgctggac ggcaagcgcg atccactgat tgctgcctat cctccggacc
     2281 ggctcctgaa ggccaaatga acctgcacac cgtttcaagt ttaagtgttc tccgggccct
     2341 ggctattgga acagcaggtg gcgccctcgg ccatgtcatc ggcattcccg cgccctggct
     2401 ggcgggaagc ctggttgcca cgatcatcgc cgtctattcc aaccagaccc tggagcttcc
     2461 ggactggttg cggaccctgg ccttcatcct gcttggcatt cagaccggaa cagcggtgaa
     2521 ttcggaaacc ttgtaccggg cggggcaatg gccgctgagc attgtgtgca tgggcgttac
     2581 cgtggggctc atcgtctggg cctgcatggt ttactatgag cggcttcgca agtggaacaa
     2641 ggccacggct tatttttcca gccttcccgg ggcgctgtcc atggtcatcc tgcttgccag
     2701 cacgtcgaag gcggacatgc ggcgggtcac catctcgcaa tgtgtgcgcc tgttcttcct
     2761 gattgccgcg ctgccgctgg tgatcgttta catttcgccg gccccccctg cgttcccggc
     2821 aacgccagcg gttgccagca ttttcgagat cattatcctc gtgggggttt cggctgcggc
     2881 gggcctgctg tttgaacgac tcagggttcc ggcgggactt attctgggag ccgcacttgc
     2941 cagtgccgcc ctgggcctcg gcgatgtgat ccatggcggt gcgcctgatt ccattctgat
     3001 cccggccaac atcattcttg gcgtcatgat tgcgctccgc ttcaagggca tttcatggcc
     3061 ggaactgcga atatcgcttg gcgacggctt tgccggcttt gccattgcga tggtcattgc
     3121 ggttgcgggg gcgctgttca ccagccagat cgcgggcctg ccgctggccc ttacgctgct
     3181 ggccttcgcg ccgggcggcc ttgaggccat gaccatcatg gcctttgccc tcaaccttga
     3241 tccggcctat gtggcggccc atcaggtggc ccgctatatc ggcctggtgc tgctcatgcc
     3301 ggcggtgacc agttttgtcc tgcaccgcaa tgtgccggcc ccgcagccgg tgaacgtcga
     3361 agaagactga cgggcgcagg gaagtctgag cgttggtctg gcaccctttc tgcacctgaa
     3421 cttgaaagcg gccagttcct tacggtactg tcccgttttg atacagtttg tgagtttggg
     3481 gagccagatt agtggccgag catgacgatc tcagcggcgc attgcgcaag gcccgcctgg
     3541 cggaagcggc gcatttcgag gcggtgctcg gcatcaagga ttccaagtca ctgcgcctcc
     3601 aggtgctgaa ggacgacatg gcccccctca tcgcggcaac accggaagcg gccaatgtct
     3661 ttgacctggc acttgttccc ggcgagccgc cgcggctgtg gattgacctt atctcctttg
     3721 tcgaaatgga gcctgactac cggacctacc gcctgcagca ggacaatcag ggtggccgcg
     3781 aagccctctt tgaaaccacg gaccgggcgg aaatggtgac atacctgaga tcttacctgg
     3841 cgcaccggat gattgcgcgc gaacggcaaa ttgtgcgggc agcgccggtt tcccagcctg
     3901 ctgcaagata taccaccggg gctgtggttt acgcctggtt caatggcctc atccttggct
     3961 tcctggccct ggtcgttgct gctatctcat tgggaaagct caagttttag gctttcatcc
     4021 gaccgttaca atctgaaaca ccatttgatt tcacctccct gactgccctt gttactcaaa
     4081 ggcaggggct taagcggatt cgctcggatt cgcgggaaca ctagatgcaa cgaagacttc
     4141 caaaggtgcg cgtacaatga ctgcgaaggc agctaaacaa gaggctgtag cggtttcggc
     4201 cgggctcgcc agaaccccgc gggcgaaccg tgacctggag cagaagcttg ccggattcac
     4261 taccattgcc gaaggccttg actatgcggc gtgcgggctg acgggtttca acttctattc
     4321 cgccaaaggc gttttgagcg acgtattgac ctacgccgag ctgcgcaggc gggcgttggc
     4381 cacggcgcgg aagctgatgt caaccgggct gaagcgcggt gaccgggtgg cggtgattgc
     4441 cgaaaccggg ccggagttca tggcggtgtt ttttggctgc cagtatgcgg ggctggttcc
     4501 ctgtccgatg ccctacacga tgtatatcgg ggggcgcgat gcttatgttg agcgggtcac
     4561 cggcatgctg cgtgctgctg ctgcctgtgc cgtcgtcacg tcgtctgatc ttgaaggtca
     4621 tatcctggcg ggcgctgccg gcgccggggt tgaaaaagtc ctgacccatg aggcgctgca
     4681 ggcgctgccg gaatcgctca ccaagcttga ggcattcggg cctgatgatg tggcctatat
     4741 ccagtattct tccggctcca cttcggaacc caagggcgtt ctgatcacgc agaaagccat
     4801 catggcaaat acccacggca ttctgcggga tggcttgcgg gttacaaaaa gcgaccgggc
     4861 attttcctgg ctgccgctct atcatgacat gggcctcgtt ggattttgcc tcgcgccgat
     4921 gatggggcag gtgtcggtcg attacatctc gacgccctca tttgcccgca ggcccgcctt
     4981 gtggctgcgg ctgatgtcgg aaaaccgctc gacgatttcc tattcgccga gcttcggcta
     5041 cgaccttgcg gcgcggcgca tcaacggcga agcggtcacg cttgacctca gcaactggcg
     5101 cgcggccggc attggcggcg acatggtgcg cgccgatgtg ctcaagaatt tcggcgacac
     5161 gctgtcggta gcaggtttca atcacaatgc cttcatgccg agctacggca tggcggaatc
     5221 aacccttgcg gtttccttca gcgacgtagc gcagcccctt cgcatcgata cgatcgacaa
     5281 gcacatgttc aagctttcgg gacgggcggt tccggcaccg gagacggcgg acaaggatgc
     5341 ggtgcgcagc ttcgtggtgt gcggcaagcc gttgcccggc catcagatgg tggtgcgcga
     5401 tgagatgggc acggtgctca gggaccgcga gatcggccac atttttgtga agggtccgag
     5461 ccttatgtcg ggctactatc acaacgcacg ggcgaccgat gcggtgatcg cggcggacgg
     5521 atttctggcc accggcgaca tgggctacat gctggatggc gagatcgtca tcaccggccg
     5581 cgccaaggac ctgatcctgc acaatggccg caatatctgg ccgcaggaca tcgagtgggc
     5641 ggccgagcag gttgaacccc tcaagtccgg cgatgtggcg gcattcgcgg ttgaaggcga
     5701 tgatggctgc gatgacgtgg tggtgctggt gcagtgccgg gtcacgacgg acgccgagat
     5761 tgaaaagctg cggcgtgaag tgtccgctgt ggtgcatcac agcgcgggcg ttgaatgcgc
     5821 cgtggttctg gtgccgccga agagtctgcc gttcacttcg tcggggaaac tgtcgcgggc
     5881 cggcgccaag cagaaatatg tttccggtga actggttgaa attacccaac gggctttcca
     5941 agcggctgac tttctggccg ctgcccagta aacacagacg aagcctgtca tgccgttgaa
     6001 aatcgccctc acaggagcca cgggatttgt tggccgggcc gtggtttccg cctttgcggc
     6061 cgagtcctgc cgcctctcag cgctggtgcg tgagcctgcg cgtgccggcc tgaatcccag
     6121 cgtgcggcag gttgagggtg atttgcaaag cttggccgcc cttgatgacc tcacccgggg
     6181 tgcggatgcc gtcattcaca tcgccggggt catcggcgcg ctcagccgcg atgaattttt
     6241 tgccgccaat gaacagggtt cgcgggcggt cgcagaagcg gcggcgcgca atggcgttgc
     6301 tcgtttcgtg catatttcct cgctggcggc gcgggaaccg gagctttccc tgtatggggc
     6361 gagcaagcgc gcgggagagg ccgcggtgga agcattcatg gaccggatgt cggtcgtcat
     6421 cctgcggccg cccgcggttt acgggcccgg cgaccgggga accctgccgc tgctgcgctc
     6481 gctcacccag tcactggcgg tcattccagg ccgcagcacg tcacgcttct cgctcatcta
     6541 tgtggatgac ctggcgcgca tcatcgtcga ggcagcaaca gccgccagag ccggcgtggt
     6601 ggaactggat gacgggcggc ggcagggcta tggctggaga gatcttgcgc acatcgccgc
     6661 ggccagcgaa cagaagagca taaagcccat ttttctgccc aaatctgttg cgatggctgt
     6721 cggcatgggc gctgaagccg ttgcaagact gaggggcaag ctgccctttg tttcgcctga
     6781 caagatccag cagctctatt tcggcgattg ggtggcgcgc ggcgaaggct ggcctctgaa
     6841 ggagccggtt gacttcgcgc ggggcttcaa gtcaaccgtg gactggtacc gcaaggcgca
     6901 gtggttgccg gagcggcccg cagcgcgcag aattgcaacg caagagagat catgactgcc
     6961 tcacaggaaa ccatcgacca gatctgcaaa ttgctggaac cgttcaatac caccggcaag
     7021 gtgctttcgc ctgaaactga gatttccggc gacctcaata tcgactcggt tgcggtgatg
     7081 gatttcgtca tggaagtcga agaccatttc gatatcgaga tcccgctgaa tgtcgtgtcg
     7141 gagacgcaca gcatcggcga tctcgccgct gtcgttgaag cccgcgtaaa gaaggcctga
     7201 tgccatgatt gatctgctgg acaagcacaa aggaattgcc gaacgccacg cccggatgat
     7261 ggcgatgggg gtgaacgcgg tgggcctcac caatgacagg atcctgtcgc cgacgcgggc
     7321 catgatcaat ggccgggaga cgatccttgc cggcaccaac aactacatgg gcatcacctt
     7381 tgacgcggac tgcgtcaagg cggggcagga tgccatggcg gaattcggca cgggcacaac
     7441 gggctcgcgc atcgccaatg gcagctatgc cttgcacaag gagcttgagg cggagctcgc
     7501 caaattcctg aagcgcaagc attgcattgt cttcaccacg ggctaccagg ccaacctggg
     7561 catgatgtcg ggcctcgcgg gtccgaagga cacgatttac ctggatgcgg attcgcattc
     7621 ctccatttat gacggctgca tagacgaagg cataaacatt attgacacgc gcc
//
LOCUS       DAORVN010000008         7599 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_5681_length_7599_cov_2.903367, whole genome shotgun sequence.
ACCESSION   DAORVN010000008 DAORVN010000000
VERSION     DAORVN010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 7599)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7599)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7599
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5681_length_7599_cov_2.903367"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1106
                     /locus_tag="PKV23_00365"
     CDS             <1..1106
                     /locus_tag="PKV23_00365"
                     /inference="COORDINATES: protein motif:HMM:NF017569.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HQX83912.1"
                     /translation="DAALSGSLRTRDEFYWTLHAILVKRREHKEIFDQAFHVFWKKPK
                     MLEQLMQLMFQQITRAAGDKPKDPGFRRLAEAMFDQKDAQSRREKPEALELDATFTAS
                     ADDILRAKDFEQMTLAEQIQAKEAIARLRLQRREFLTRRFQPDHAGARIDMRRTLRQS
                     LRAGGHMIDLARRAPKIRQRPLVVLCDISGSCSSYSRMFLHFLHGLANDRTDVTVFLF
                     GTRLTNITRELQHLDVDESIAKVSSAVKDWSGGTRIGATLREFNYKWARRVLTQGADV
                     LLMTDGLERDDIVQLSQEMQRLRRSAKKIVWLNPLLRFDRFEAKASGIRVMMPYVDEF
                     RPVHSLNSLSDLAEALGKRAGPAHDPRKWLKAA"
     gene            1125..1559
                     /locus_tag="PKV23_00370"
                     /pseudo
     CDS             1125..1559
                     /locus_tag="PKV23_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018389537.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase subunit G"
     gene            complement(1730..2914)
                     /locus_tag="PKV23_00375"
     CDS             complement(1730..2914)
                     /locus_tag="PKV23_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006202444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HQX83913.1"
                     /translation="MLTNNTDVLIVGAGPTGTTLAIALQQAGVNHLLIDKLEQGQNTS
                     RAAVIHAHTLEMLEELGVSGELAKRGMKLANFVIRDRNRALVHLRFDQLPSAYRYLLM
                     LPQNETEKVLAERLAALGGSIRRGITATAVSQHADGAQVTLKSPKGDTFVNARYVVGG
                     DGMHSIVRTAVGIEFEGGTYEESFVLADVRMQWSLGTSEVSLFFSPAGVVVVAPLPDG
                     AYRIVATLDNAPEQPGIADIQALLDARGPTTGLNRVEEVVWSSRFRVHHRIARSYRKG
                     PFLLMGDAAHVHSPAGGQGMNTGIVDAVVLGRLLADVIHGKQPDAVLDSYGQVRRPAA
                     EKVLGLAGLLTSMATVRSAPKRTLRNAVLSLINRLPPARRRLQMNLSGLARRQMAAIP
                     GR"
     gene            2996..3571
                     /locus_tag="PKV23_00380"
     CDS             2996..3571
                     /locus_tag="PKV23_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006202443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HQX83914.1"
                     /translation="MDQELKKPGKADRTRAAILAAAQQLFAGQGYELTTVRDIAAIAD
                     IDPAMVIRYFGSKDELFARAAVFNLKLPDLSKAERSEIGETLVRHFLSLWEGENANGA
                     MPILLRSAASNETAANKLREIFMGQVLPALTHAGGRSGAAERAGLVSSQLLGLALCRY
                     VLKLPPVVGLSQDMIIREVGRTIQRYATGEQ"
     gene            complement(3626..4786)
                     /locus_tag="PKV23_00385"
     CDS             complement(3626..4786)
                     /locus_tag="PKV23_00385"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HQX83915.1"
                     /translation="MKMRCAALALVVAASMAAGSILPVPAWANKTMSCGSPLEAQGEW
                     QTASPEAAGFDSALLCSLGDKLDTSPALNVHAVVVVRSGKLVYETYRTGEDQRWGTGL
                     GLASHTPQTLHDVRSVSKSVVSLLVGIALDRKLVASINTPVFSYFPELAALRTPEKDR
                     IELRHLLAMTSGLAWDERRPYSDPENSERRMTVAANPYRFALEPPVVNAPGEKWNYNG
                     GNTQLLAGVLERSAGMWLTDFASEELFVPLGIKQFEWLKMWANGDVAAAYGLRLRPRD
                     MAKLGQLVLDKGLWNGRRIVSEAWIRESTQPWADVAPPLRYGYQWWIDEGEIAGRKIS
                     WFSAQGLGGQRIYIVPALGLVVVITAGLYESSSQDWVSFDIFDKFVLAAIRK"
     gene            complement(4800..6176)
                     /locus_tag="PKV23_00390"
     CDS             complement(4800..6176)
                     /locus_tag="PKV23_00390"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQX83916.1"
                     /translation="MQSNSLSFRLIFTSAVVAVVLLVTAAFLLAYLFQQALERNFDAR
                     LRAILDGLLANVELATDGSPVMSGKISDPRFTIPLSGWYWQVSPPSHRTLPDLASESA
                     LEQRLTPTGVDLQTRGSDGVASFYIKDSNGQQLRAIEQKFKLFGGEEFSFLVAGNFDE
                     LKAEVTAFQRALYAVLTLLGLGLLLAIFLQVRVAMQPMQRMQQSLAAIRAGKAERLEG
                     EFPHEMQPVADELNLLILAGTEIIDRARTQVGNLAHALKTPLSVLSNEAQGNKSDFGA
                     KVTEQIQIMRDHVSLYLDRARRAARAQTLGAATDVEPVLAALGRTIMRINKDRGVTIE
                     VTVTPGLKFRGEVQDLEEMAGNLIDNAAKWSRQAVKISAAPVIDPIDGTQNWLMITVD
                     DDGPGIAASKRAEAVKRGKRLDETKPGSGLGLSIVTETAGMYSGNLVLGDAPMGGLRV
                     QLRLPAVA"
     gene            complement(6166..6837)
                     /locus_tag="PKV23_00395"
     CDS             complement(6166..6837)
                     /locus_tag="PKV23_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HQX83917.1"
                     /translation="MRILVVEDDPDLNRQIVAALGDAGYVVDKAMDGEEGHFLGETEP
                     YDAVVLDLGLPVLDGVSVLEKWRRAGKKMPVLILTARDRWSDKVAGFDAGADDYVVKP
                     FQMEEVLARMRALLRRSAGHATSELTCGPMVLDTKSARVTVNGTAIKLTSLEFRLLSY
                     LMHHGGKVVSRTELVEHLYDQDFDRDSNTIEVFIGRLRKKLGVDVLHTIRGMGYCVSE
                     PDNAK"
     gene            complement(6873..7187)
                     /locus_tag="PKV23_00400"
     CDS             complement(6873..7187)
                     /locus_tag="PKV23_00400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HQX83918.1"
                     /translation="MLRGLKFVAVALAALWVAEPAIAQYQPNRPQILRKLAPNVIKPN
                     LNLLPEVRIAPSQAAAIAQADYPGSRVLKVRQRGGVYIVTLAVDGSVIRVRVDGTHGS
                     IL"
BASE COUNT         1557 a         2265 c         2359 g         1418 t
ORIGIN      
        1 tggatgcggc gctgagcggt tcgctgcgga cccgcgatga attctactgg acgctccacg
       61 caatcctggt gaagcggcgc gagcacaagg aaatctttga ccaggcattc cacgtcttct
      121 ggaaaaagcc gaagatgctg gaacagctga tgcagctcat gttccagcaa atcacccggg
      181 cggcgggcga caagccgaag gacccgggtt tccgcaggct ggcagaggcc atgttcgacc
      241 agaaggatgc gcagagccgc cgggaaaagc cggaggcgct ggaacttgat gcgacgttca
      301 ccgcatccgc cgacgacata ctccgcgcca aggatttcga gcagatgacg ctggctgaac
      361 aaattcaggc gaaggaagcg atcgcccgcc tgcgcctgca gcggcgcgaa ttcctgacac
      421 ggcgctttca accggatcat gcgggcgcgc gcatcgacat gcggcggact ttgcggcaat
      481 cgctgcgcgc gggcgggcac atgatcgatc tggcgcggcg cgcgcccaaa atccgccagc
      541 ggccgctggt cgtgctctgc gacatttccg gctcgtgctc gagctacagc cgcatgttcc
      601 tgcattttct gcacgggctg gcgaacgatc ggacggatgt gaccgtgttc ctcttcggca
      661 cgcggctcac caacatcacc cgcgaactgc agcatcttga tgtggatgaa tcgatcgcca
      721 aggtttcttc ggccgtgaag gactggtcgg ggggcacgcg gatcggcgcc acgctcaggg
      781 agttcaatta caaatgggcg cgccgcgtgc tcacccaggg tgctgacgtg ctgctgatga
      841 ccgacggact ggagcgcgat gacatcgtgc agctttcgca ggaaatgcaa cgcctgcgcc
      901 gcagcgccaa gaaaatcgtc tggctcaatc cgctgctgcg ctttgaccgc ttcgaggcaa
      961 aggcgtccgg catccgcgtc atgatgccct atgtcgatga attccgcccg gtgcacagcc
     1021 tcaattcgct cagtgatctg gcggaggctc ttggcaagcg ggcgggcccg gcgcatgatc
     1081 cgcgcaaatg gttgaaggca gcgtaaaaat gaggaattcg tgaaatgaaa atgtcaggca
     1141 gccaggtcat cgacgcgccc cgcgacgcgg tatggaaagg cctcaacgat ccaaaggtcc
     1201 tgcagcaatg catcccgggc tgcgagagca tcgtggcgtc atcgcccacc cagatgagcg
     1261 ccaaggtggt gctgaaaatc ggcccggtga aggcggcgtt caagggttcg gtgacacttt
     1321 ccgacatcaa ggcgcccgag agctaccgga tttccggcaa gggcgaaggc ggctttgcag
     1381 gctttgccag cggcggggcc acggtgaagc tcacggcgaa cagcccgagc gagaccctga
     1441 tggaatatga cgtggacgcg caggtcggcg gcaagatcgc catgctgggc tcgcgcctgg
     1501 tcgactccac cgcgcagtcg ctggccaacc agttcttcga gagattcgcc gccgtggtga
     1561 aacagagcgc tgccgctgtt cccaaaaaac caaaggccaa ggccgccaag aagcccgcga
     1621 aaaagccagc ggccaagaag ccggtaaagg ccaaaaaggt cgcggccaag gcgaagaaaa
     1681 aaccggcgaa gaagaagcgc tagaatccgc cctgccgcaa ctctccgggt caccttcctg
     1741 gaattgccgc catttgcctg cgggcaagac ctgagagatt catctgcagg cgccgcctgg
     1801 cgggcggcaa gcggttgatg agcgacagga cggcattgcg cagggtccgc ttcggagcgc
     1861 tgcggaccgt tgccatgctg gtgagcaggc cggcaaggcc cagaaccttc tccgccgcgg
     1921 gccggcgcac ttggccatag ctgtcgagca cagcgtcggg ctgcttgccg tggataacat
     1981 cggccaggag ccgaccaagc accaccgcgt cgacgatccc ggtgttcatg ccctggccgc
     2041 cggccggact atggacatgg gccgcatcgc ccatcagcag gaacggaccc ttgcgatagg
     2101 acctggcgat ccggtgatgg acacggaagc gggaactcca gacaacctcc tcaacgcggt
     2161 taaggccggt cgtggggccg cgcgcatcga gcaaagcctg gatatcggca atgcccggct
     2221 gttcgggtgc attgtcgagg gtggcgacaa tgcggtacgc gccatcgggc agcggggcca
     2281 cgacgaccac gccggcgggc gagaagaaca gcgagacctc cgaggtgcca agcgaccact
     2341 gcatgcgcac gtcagcgagg acaaacgact cctcataggt gccgccttcg aattcgattc
     2401 ctaccgccgt gcggacgatg ctgtgcatcc cgtcgccgcc cacgacgtaa cgggcgttca
     2461 caaaagtgtc tcccttcggc gacttcaacg tgacctgcgc cccatcggcg tgctgtgaca
     2521 cagccgttgc cgtgattccg cgccggatcg atccgccaag ggccgcgagg cgttcggcta
     2581 ggaccttctc ggtctcgttc tggggaagca tcagcagata gcgataggct gacggcagct
     2641 ggtcaaagcg cagatgcacc agggcgcggt tgcggtcgcg gatgacgaag tttgcgagtt
     2701 tcatgccgcg cttcgccagt tcaccggaaa cgcccagttc ctcaagcatt tcaagggtat
     2761 gggcgtgaat gacagcggcc ctcgaggtgt tctggccctg ctcgagcttg tcgatgagca
     2821 gatggttcac acccgcctgt tggagggcga ttgccagggt ggtgccggtc ggccctgccc
     2881 caacgatgag tacgtctgtg ttgtttgtaa gcattgttta actccttttg tcaacggttg
     2941 ttgacttact aaacaaggct attgcatttg tcaacacttg ttgacattat gccgtatgga
     3001 ccaggaactg aaaaaaccag gcaaggccga ccgcacccgc gcagccatcc ttgccgctgc
     3061 ccagcaactc tttgccggac agggctatga gctcacgacg gtgcgcgata ttgcggcgat
     3121 cgccgacatc gatccggcaa tggtcatccg ctatttcggc agcaaggacg agctgttcgc
     3181 gcgggccgcg gtgttcaacc tcaagctgcc tgacctcagc aaggccgagc gttccgagat
     3241 tggcgaaaca cttgtgcggc attttctcag tctgtgggaa ggagagaacg ccaatggcgc
     3301 gatgccgatc ctgctgcgct cggctgcttc gaatgaaaca gccgcaaaca agctgcggga
     3361 gatttttatg ggccaggttc tacccgctct aacccatgca ggaggccggt caggcgcggc
     3421 ggaacgggca ggacttgttt cgagccagct gctcggactt gccctctgcc gttacgttct
     3481 caagcttcca ccagtggtcg gcctgtcgca ggatatgatc atccgcgagg tcggccgcac
     3541 gatccagcgc tacgctacgg gggaacaata agtgcgaacc cgcgcggaag ccaggtttca
     3601 aggctccgca cattttggcg gcgaattact tgcgtatggc agccagcacg aatttatcga
     3661 agatgtcgaa ggaaacccaa tcctgactgg aactctcata aagtcctgct gtgatgacga
     3721 cgacgaggcc taaggcggga acgatgtaga tgcgctgccc gccgaggccc tgggcggaga
     3781 accaggatat tttccggcca gcgatttcgc cttcatcgat ccaccattga tagccatagc
     3841 gaagcggagg cgcgacgtcg gcccaaggct gcgttgattc cctgatccac gcctccgata
     3901 ctatgcggcg gccgttccac aggcccttgt cgagaacgag ttggcccagt tttgccatgt
     3961 cgcgtgggcg caggcgcagg ccgtaagcag ccgcgacatc tccattggcc cacatcttca
     4021 gccattcaaa ctgttttatg ccaagcggca cgaacaattc ctcacttgca aaatctgtca
     4081 gccacatgcc ggccgatctt tccagcacgc cagcgaggag ttgagtattg ccgccattgt
     4141 agttccattt ttcgccgggc gcgttcacga ccggtggttc gagcgcaaag cgatagggat
     4201 tggccgcgac tgtcattctc cgttcgctgt tttcggggtc gctgtagggg cgccgctcgt
     4261 cccacgccag ccctgacgtc atggccagca gatgacggag ctcaatgcgg tctttttccg
     4321 gcgttcgaag cgctgccaat tcagggaagt atgagaatac cggggtatta atgctggcaa
     4381 caagcttccg gtccagcgca atgccgacaa gcagcgatac aacgctcttg ctgaccgaac
     4441 gcacatcatg gagtgtttgc ggggtgtggg atgcgaggcc caggccggtg ccccatctct
     4501 gatcttcacc ggtgcgatag gtctcataga caagcttgcc gctgcgcacg acaacgactg
     4561 catgcacatt caatgcgggc gaagtgtcga gcttgtcgcc cagcgaacaa agcagcgcgg
     4621 aatcgaaacc ggctgcctca ggggaagcag tctgccattc accttgggcc tcgagcgggc
     4681 tgccgcatga catcgtcttg tttgcccatg ccggaacagg caatatagat cctgcagcca
     4741 tgcttgccgc caccacaagg gccagagctg cacatcgcat cttcatgggc agctccgatt
     4801 cacgccacag ccggcagccg caactggact cttaaaccgc ccattggcgc atcgccaaga
     4861 accaggttgc cgctgtacat gcccgcggtt tcggtgacga tggaaaggcc caggccggag
     4921 ccgggtttgg tctcgtcgag gcgtttgccg cgcttgaccg cttcggcgcg cttgcttgcg
     4981 gcaatgcccg ggccatcgtc atcgacggtg atcatcagcc agttctgggt gccgtcgatg
     5041 ggatcgatga cgggcgcggc actgatcttc acggcctgcc tggaccactt ggcggcgttg
     5101 tcgatgaggt tgcctgccat ttcctcaagg tcctggacct cgccgcggaa cttcagtccg
     5161 ggggtgacgg tcacctcgat ggtgacgccc cggtccttgt tgatgcgcat gatggtacgg
     5221 ccgagggcgg cgagcaccgg ttccacatcc gtggccgccc ccaaagtttg ggcgcgggcg
     5281 gcgcggcgcg cccggtcgag gtagaggctc acatggtcac gcatgatctg gatctgctcg
     5341 gtgaccttgg cgccgaaatc cgacttgttg ccctgggcct cgttgctgag aacactcaag
     5401 ggcgttttca gggcatgggc caggttgcca acttgcgtgc gggcccggtc gatgatttcc
     5461 gtgccggcca ggatgagcag gttcaattcg tcagccaccg gctgcatttc gtggggaaac
     5521 tcgccttcca ggcgctcggc cttgccggcg cggatggcgg cgagggactg ctgcatgcgc
     5581 tgcatcggct gcatggcgac gcgcacctgg aggaaaatgg caagcagcag gccgaggccc
     5641 aggagggtga gcaccgcata aagggcgcgc tggaaggcgg tgacttccgc cttcagctca
     5701 tcaaaatttc cggccaccag gaaggaaaat tcctcgccgc caaagagctt gaatttctgt
     5761 tcgatggcgc ggagctgctg gccgttgctg tccttgatgt agaagctggc cacgccatcg
     5821 gacccacggg tctgcaggtc aactccggtg ggtgtcaggc gctgctcgag cgcggattcg
     5881 gacgccagat caggaagggt tctatggctg ggcggcgaga cctgccaata ccagccagac
     5941 agcggaatgg tgaagcgtgg atcggaaatc ttgcccgaca tgaccggaga gccgtcggtc
     6001 gcaagttcca cattggccag gaggccatca agaatggcgc ggagcctcgc atcgaaattg
     6061 cgttcaaggg cctgctggaa aaggtaggca agcaggaagg cagccgtgac aagcaggacg
     6121 acggctacga cagcggaggt gaagatcagc cggaaagaga gtgagttact ttgcattatc
     6181 cggctcggat acgcagtatc ccatgccgcg gatagtatgc agcacgtcaa cgccgagctt
     6241 tttccgcaag cggccgatga acacctcgat ggtgtttgaa tcgcggtcga agtcctggtc
     6301 atagaggtgc tcaaccagtt cggtgcgcga aaccaccttg ccgccgtggt gcatgagata
     6361 ggacagcagg cggaattcca gcgaggtgag cttgatcgcc gtgccgttca cggtcacccg
     6421 ggcgctcttt gtgtcgagca ccatggggcc gcaggtgagt tcgctggtgg cgtggccggc
     6481 gctgcggcgc aaaagggcgc gcatgcgggc cagcacttct tccatctgga agggtttgac
     6541 cacataatca tcggcgccgg cgtcaaagcc cgccaccttg tcgctccagc ggtcccgcgc
     6601 cgtgagaatc agcaccggca tcttcttgcc ggcgcggcgc catttttcca ggacgctgac
     6661 gccgtcgaga accggcaagc caaggtccag gacaaccgca tcatagggct cggtctcgcc
     6721 gaggaagtgc ccctcctcgc catccatggc cttgtctacc acgtagcctg cgtcaccgag
     6781 ggcggccaca atctgccggt tcaggtctgg atcgtcttca acgacaagta tgcgcattta
     6841 tctccatccc agtgtggcag gcagcccggt gactatagga ttgaaccatg ggtcccgtcc
     6901 accctgacgc gaatcacgct gccatcaacc gccagggtga caatatagac gccgcccctc
     6961 tgccttacct taagtaccct ggagccggga tagtcagcct gggcaatggc ggcggcctgg
     7021 gatggcgcga ttcgcacttc cggcagcagg ttcagattgg gcttgataac attgggcgcc
     7081 agctttcgca ggatctgcgg acggttgggc tggtattggg caatagcggg ctcagcgacc
     7141 cacaaagccg ctagcgcaac tgccacaaac ttcagacctc tcaacattaa acctgtcgcc
     7201 tttcctccag ccgttctagt tggcgctagc tgaactgggg atgaatagaa atgtcaggat
     7261 tgttgaccaa tattaaactt tagtcataac aatcggttag gaagataatt tcctgcacca
     7321 gaaggttatt aaagggcgga tttgcccgcc acattcttgg cctcccatcc cctcctcaaa
     7381 atgggatgat gaaaccgtct aatcaaggaa cagaaatggc cccagcgcgt tcagagagcc
     7441 gggaaccaga tgaacggcag atgaatgccg ccttcaagtg ccatacaggc ccggggcggt
     7501 aatcatgtgc tcaacactta accaaggacc agagagacaa ccatgaacgc ccccgccgcc
     7561 cagaacttca agctggtgtc atggcgcgag gtccgcaag
//
LOCUS       DAORVN010000009         7451 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_5896_length_7451_cov_2.985803, whole genome shotgun sequence.
ACCESSION   DAORVN010000009 DAORVN010000000
VERSION     DAORVN010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 7451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7451
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5896_length_7451_cov_2.985803"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..338
                     /locus_tag="PKV23_00405"
     CDS             <1..338
                     /locus_tag="PKV23_00405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HQX83919.1"
                     /translation="HSRPLVLIWGMLNTKDAASFIEPFEGLAQRIVTIAIPGEPNAVP
                     ADQLAEIARSRNLSAETASSLEKALGQASQTTPAPRILICGSLYLAGHVLSLHDNKPM
                     SGVSGAARR"
     gene            complement(335..655)
                     /gene="trxA"
                     /locus_tag="PKV23_00410"
     CDS             complement(335..655)
                     /gene="trxA"
                     /locus_tag="PKV23_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007598398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HQX83920.1"
                     /translation="MSTHKVSDASFDADVLKSTTPVVVDFWAEWCGPCKMIGPALEEI
                     AQTLGPKVKIVKINIDENPQTPSKYGVRGIPTLMVFKGGAVSSTKVGAAPKGQLQSWI
                     EQSI"
     gene            complement(732..3050)
                     /locus_tag="PKV23_00415"
     CDS             complement(732..3050)
                     /locus_tag="PKV23_00415"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HQX83921.1"
                     /translation="MIAEFEAAKRFRGAYDFDDLILKTRELLVDKATAQWVLFKLDRG
                     IEHVLVDEAQDTSLSQWQILMALTEEFFSGSGSRAAPDRTLFVVGDRKQSIFSFQGAD
                     PDAFEESRAFFEQKITDVGQLFKPVDLTVSYRSTAEVLKSVDAVFAEGTRARVGLDGS
                     HAQTLLHQSNRGNAPGLFELWPLMEADDKAEREPWQAPVDQYPPKSAVRKLARRLAET
                     IKSWIGKRHIMALNRAVEAGDILILFRTRNSLFDILISELRGAGIPVAGADRLKLSEN
                     IAILDMMAMVQFVLLPEDDYSLACLLKSPVVPAPLSEDQLFKLAHGRGAASLWARLGA
                     SDDPICGMARAVLEQWLQASEGARPFEFLSTVLIGTRKAFLARLGSEAGDALDALLEI
                     SLSYEESHSSSLAGFAAWFASEDSEIKRNMDRGRGEVRLMTVHGAKGLESSIVILPDT
                     TSTPRSTATSALMFVEPVPGAPALPFWRLSGLAQSQPIEYWMATDKQTELEEQRRLLY
                     VAMTRARDELYVCGYKVHRDSPENSWYRLVMEALQNQDLRPVELPDGSSCLRLGPDPT
                     VNGMAPVLPRSAPEVPAWLFNSPDIAHHGPAPAPGRRDAGSRVARARGRAIHKILQDL
                     PGMAPDAGLNFARRIVARSGLDESLAEKILGLIADPEHRDFFGPGSQAEVSVGSLQPN
                     GQYITERIDRLVIRASDILVLDYKTDWHVPDRLDAGHPHVMQLARYVRTLGQAYDGRP
                     VRAAILWTSLPRLDWIAEDTLREAISSMAAIT"
     gene            complement(3047..4105)
                     /locus_tag="PKV23_00420"
     CDS             complement(3047..4105)
                     /locus_tag="PKV23_00420"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HQX83922.1"
                     /translation="MAQTMHATPEQKTASNPEASAWVGANAGSGKTHVLVDRVTRLML
                     AGVDPMSILCLTFTKAAAKEMANRLHERLGGWVGLSNADLSERLTQMGHDQVDRKTLA
                     RARRLFTTALETPGGLKIQTIHAFCERILQLFPVEAGLAPGFAVLESSQSKQLLRQAR
                     DHVLRLAQSAEGSLLARALEIITRHTHSDSFDGLLERLLSERQSLKDALAEHGSIDGI
                     GEALRRQLGVSPEQSIDTLREQFLMFDREAIEGIIPLLSAAQATNQKTAHNFSALLAE
                     TDSAKAEALYRRIYYGVDGEKPKAITSVITEKLMKDNPWIRDWLSEEMRRSLSIIEQI
                     DTSPALKRPAPCSRSPRR"
     gene            complement(4105..7116)
                     /gene="addB"
                     /locus_tag="PKV23_00425"
     CDS             complement(4105..7116)
                     /gene="addB"
                     /locus_tag="PKV23_00425"
                     /inference="COORDINATES: protein motif:HMM:TIGR02786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="double-strand break repair protein AddB"
                     /protein_id="HQX83923.1"
                     /translation="MRRERLPRVFTIASDAPFLDILAKAVLQGFPYAEDSPEKHPSLA
                     AWTILVPTRRAARALQSKLLMESGRQALVLPQIRPIGDLDEDMLETQRPASGLQDAIS
                     DVGREFALMALIDDWARENPQLRLAQELADAPQQAQALAASLGELIDDMETEEVSFER
                     LPEAYDIDLAVHREAILSLFDLVRRKLPAQLVMEKLMGAKERRSRLIHLEAKRLAANP
                     PAGPMIAAGSTGTIPATRELLKAISALESGAVILPGLDQEMDEKSWTAVSPQHPQYAI
                     KQLIDFMGVERKNVATLGTAGGDRAWLASELMRPSDVSDDWQAALAGQGSRIKRALEN
                     VTLAEARDKNDEAAIISVMMRQSLEKPSGDMALVTPDRDLARRVKANLARWNIRVDDS
                     AGEPLSRFSAPSLLLLLMDAVEENFSASSLRSLFSHHLSRFGFERAGFSAAARKVEVA
                     LFRSSPMIHGLEGLLPSFDLAFANSKGEAHPHPIVANLSEDDCREMRDCLARVVEILG
                     PLSHPAVTSLTMHLDGLISVAENISGDGFWDGVESDALEELIETLLREAPRLPACDFA
                     RASATIRRHLQRLPVRDTRNTVTRLAILGLLEARMIHPENIILGGLNEGTWPRLPDPG
                     PWLNRPMRDTFGMQQPERNIGQSAHDFVQAFGGTHVFLTWARRDGMDPAIPSRWILRL
                     QTILKAAGHKSGDLPTDPWQAWAARLDQPDHPQPKPQGKPQPRPPVEARPNRISVSRV
                     ETLIRDPYAIYADAVLKLRPLPDMAAAPDAALRGTLFHKAIGDFFTFYPKALPDDAVA
                     RFIALGEEIFRPFRNDAEIMGFWWARFRRLARWIVENEAVLRSDAVHVFAEVKGLLEL
                     KIGATDFKLSARADRIDILADGTARIVDFKTGAIPSKPKIEEGFSPQLTLEAAMLERG
                     AFEHVGGRETSALTYIRITGGIPPGELKQIDLAPMAVAREHLARLEGLLGKYQNPALA
                     YLPRYAIENVEAAHDYDHLSRYREWILAGDG"
     gene            complement(7117..>7451)
                     /locus_tag="PKV23_00430"
     CDS             complement(7117..>7451)
                     /locus_tag="PKV23_00430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HQX83924.1"
                     /translation="AAWDDAAMDCLLLLCHVDRTIGYDGRGDFQLGRDGRLTRQQGPG
                     ALAYIGGYLVHPRLFAGTAVKNFSMNVLWDKAISEGRLHGIAHSGKWFHVGTPAAVAL
                     AEAELAKI"
BASE COUNT         1415 a         2332 c         2288 g         1416 t
ORIGIN      
        1 gccactctcg cccgctcgtt ctcatctggg gcatgctcaa cacgaaagat gcggcgagct
       61 tcattgagcc ctttgaaggc ctggcgcaac gcatcgtgac cattgccatt ccgggcgagc
      121 ccaatgccgt tcccgctgac cagctcgcgg agattgcccg cagccggaac ctgtcggccg
      181 aaaccgcaag ctctcttgaa aaagctttgg ggcaggcgtc gcaaacgaca cctgccccga
      241 gaatactgat ttgcggttca ctctatctcg caggccatgt gctgagcctc cacgacaaca
      301 agccgatgtc gggcgtatcg ggcgcggcca ggcgttagat cgattgctca atccaggatt
      361 gcagctggcc cttgggagca gcgccgacct tcgtcgagga caccgccccg cccttgaaca
      421 ccatcagggt gggaatgccg cggaccccgt atttcgaagg agtttgcgga ttttcatcga
      481 tgttgatctt gacgattttg accttgggcc caagcgtctg ggcaatttct tcaagtgcgg
      541 gaccgatcat tttgcagggt ccgcaccatt ccgcccagaa atcgacaacg accggagtcg
      601 tggatttgag gacatccgca tcgaatgagg cgtcagatac tttatgggtg gacacgattg
      661 attctccgct attccttgag ttgccactaa aggtaggtat cgtcagcccc ccggtcaagg
      721 gcggcactcg ttcacgtgat ggctgccatg ctggaaatgg cttcccgcag cgtatcttcc
      781 gcaatccagt caaggcgcgg caggctcgtc cagagaatgg cggcgcggac gggcctgccg
      841 tcataggcct gccctagggt ccgcacatag cgggccaact gcatgacatg gggatgcccg
      901 gcatcgagcc tgtccggcac atgccagtcg gtcttgtagt cgagcacgag aatgtcgctg
      961 gcccggatga caagccggtc gatccgttcg gtgatgtact ggccgttggg ctgaagagag
     1021 ccgacgctga cttccgcctg gctgcccggc ccgaaaaaat ccctgtgctc gggatccgcg
     1081 ataagcccca ggattttttc tgccagcgat tcatcaagcc cgctcctggc gacaatgcgc
     1141 cttgcgaaat tcaggccggc gtccggcgcc atgccgggca ggtcctgcag gattttatgg
     1201 atggcacgcc cgcgggcacg ggccacacgc gagccagcgt cacggcgccc aggcgccggg
     1261 gccgggccgt gatgcgcaat atccggacta ttgaacagcc aagcaggaac ctcgggagct
     1321 gatcttggca aaaccggggc catcccgttc accgtcggat cagggccaag ccgcaggcaa
     1381 gagctcccgt caggtaattc gaccggccgc aagtcctgat tctgcagcgc ttccatcacc
     1441 aggcgatacc agctgttctc cggactgtcc cgatggacct tgtagccgca gacataaagc
     1501 tcatcgcggg cccgcgtcat ggccacatag aggagccgcc gctgctcctc cagttcggtt
     1561 tgcttgtctg tggccatcca gtattcaatg ggctgggatt gcgccagccc cgagagccgc
     1621 cagaaaggca gcgccggagc acccgggact ggctcgacaa acatgagggc cgaggtcgca
     1681 gtgctgcgcg gcgtggaggt ggtgtccggc aggatgacga tgctggactc cagccccttc
     1741 gcgccatgca ccgtcatcag gcgcacttcg ccgcggccgc gatccatgtt gcgcttgatc
     1801 tcgctgtcct cggaggcaaa ccaggccgca aaccccgcca gcgaagagga atggctttcc
     1861 tcataggaga gcgagatctc aagcagcgca tcgagtgcat cgcccgcctc gcttcccagc
     1921 cgcgccagga acgcctttcg cgttccgatc agaaccgttg acaggaattc aaagggtcgc
     1981 gccccttccg aagcctgcag ccattgctcc agaaccgcgc gggccattcc gcagatcgga
     2041 tcatcggagg cgccgagccg cgcccagagg gaagccgcac cgcgtccatg ggccagtttg
     2101 aaaagctggt cttccgacag gggcgccggc accacagggc ttttgaggag gcacgcgagg
     2161 gaataatcat cctcgggcag gagcacaaat tgcaccatgg ccatcatgtc gaggatggcg
     2221 atattctccg aaagcttcag ccggtcggca cccgccaccg gaattccggc cccgcgcaat
     2281 tcgctgatca ggatatcgaa cagcgaattt cgcgtccgga acaggatcag gatgtcgccg
     2341 gcctccaccg cccggttgag cgccatgatg tggcgctttc cgatccacga cttgatcgtt
     2401 tccgcaagcc gccttgccag tttgcgcacg gcggatttgg gcgggtattg gtcgaccggc
     2461 gcctgccagg gctcccgctc ggccttgtca tcggcctcca tcagcggcca gagttcaaac
     2521 aatccgggcg cgttcccccg gttggactga tgcagcaggg tttgcgcgtg agacccatcc
     2581 agccccacgc gggcccgcgt tccttcggca aagaccgcgt cgactgactt cagcacctcg
     2641 gcggtggagc ggtaggaaac cgtgaggtcg acaggcttga agagttgtcc cacatcggtg
     2701 atcttctgtt cgaagaaggc ccgtgactct tcaaaggcat ccgggtcggc gccctggaaa
     2761 ctgaaaatcg actgcttgcg gtcaccgacc acgaaaagcg tgcggtccgg cgccgcgcgg
     2821 ctgccgctgc cggaaaagaa ctcttccgtc agcgccatga gaatctgcca ttgggaaagg
     2881 ctggtgtcct gcgcctcatc gacaagcaca tgctcgatgc cccggtcgag cttgaacaga
     2941 acccattgcg ccgtcgcctt gtcgacgagc agctcgcggg ttttcaggat gagatcatcg
     3001 aagtcataag cccccctgaa tctcttggcc gcttcgaatt ccgcaatcat cgccgtggcg
     3061 agcgtgagca gggcgcaggt cgcttcaatg cgggtgaggt gtcgatctgc tcgataatgc
     3121 tcaggctgcg ccgcatctcc tcagagagcc agtcccggat ccagggattg tccttcatga
     3181 gcttttcggt gatgacggaa gtgatggctt tcggcttttc gccatctacc ccatagtaga
     3241 tgcgccgata aagcgcctcg gcctttgcgc tgtctgtctc tgccagcagg gctgaaaaat
     3301 tgtgcgcggt cttttggttg gtggcttgtg cggcggagag tagcggaatt atcccctcga
     3361 ttgcttccct gtcaaacatc aggaactgct ccctgagcgt atcaatgctc tgctcagggc
     3421 taactcccag ctgacgacga agcgcctcgc cgatgccgtc gatcgagcca tgctctgcca
     3481 aggcatcctt taggctctgg cgctctgaaa gcaggcgctc cagcaatccg tcaaaactgt
     3541 cggaatgggt gtggcgggtg atgatctcca gggccctggc aaggagcgaa ccttccgcgc
     3601 tctgggccag gcggagcaca tggtcgcgcg cctggcgcag aagctgtttg gattggctgg
     3661 attccagcac tgcaaagccc ggcgcaagtc ccgcctccac cgggaaaagc tgcaggatcc
     3721 tttcgcagaa ggcatggatg gtttgaatct tgaggccccc gggtgtttcc aacgccgtcg
     3781 tgaaaagccg ccgggctctt gcaagcgttt ttcggtcaac ctggtcatgc cccatctgcg
     3841 tgagccgttc ggagaggtct gcgttggaaa gccccaccca gccgcccagt ctttcatgca
     3901 ggcggtttgc catttccttg gccgccgcct tggtgaaggt gaggcaaagg atagacatgg
     3961 ggtcaacgcc cgcaagcatg aggcgcgtca cccggtccac cagcacatgg gtcttgcccg
     4021 aacccgcatt ggcgccgacc caggcggacg cctctgggtt ggaggcggtt ttctgttccg
     4081 gcgtggcatg catcgtttgg gccatcagcc gtctcccgcc agaatccatt cgcggtagcg
     4141 cgagagatgg tcgtagtcat gcgccgcttc aacattttcg atggcatagc gggggagata
     4201 ggcgagagcg ggattctgat atttgccgag aagcccctca aggcgggcga gatgctcacg
     4261 cgccacagcc atgggagcaa ggtcgatctg cttcagttcg cccggcggaa ttcccccggt
     4321 gatcctgatg taggtgagcg cgctggtctc gcggcccccc acatgctcaa aggcgccgcg
     4381 ttccagcatg gcggcctcca gggtgagctg cggcgaaaac ccttcttcta tttttggctt
     4441 gctcggaatg gcccccgtct tgaaatcgac aatgcgcgcc gtgccatcgg cgaggatgtc
     4501 gatacggtcg gcccgtgcgc tcagcttgaa atccgttgcg ccgattttca gctcaagcag
     4561 ccccttcacc tcggcaaaga catgaaccgc gtcgctgcgc agcacggctt cgttctcgac
     4621 gatccaccgg gccaggcgcc ggaacctcgc ccaccagaaa cccatgatct ccgcatcgtt
     4681 ccggaagggg cggaaaatct cctcgccaag ggcaatgaag cgcgccaccg catcatccgg
     4741 caaggctttc ggataaaacg tgaagaaatc gccgatcgcc ttgtggaaaa gcgtgccgcg
     4801 aagcgccgcg tcaggagccg ccgccatgtc gggcagcggc ctgagcttca gcaccgcgtc
     4861 cgcatagatc gcgtaaggat cgcggatcag cgtttcgacc cggctcacgc tgatgcgatt
     4921 tggccgggct tcaaccggcg ggcggggttg cggcttgccc tgcggcttgg gctgcggatg
     4981 atccggctgg tcaagccgcg ccgcccacgc ctgccatgga tcagtaggca aatccccgga
     5041 cttgtggccc gccgccttca gaattgtctg caggcgcaat atccaccggg agggaatggc
     5101 ggggtccatg ccgtcacgcc gcgcccaggt gagaaagacg tgggttccgc caaaagcctg
     5161 gacaaaatca tgggccgatt ggccaatgtt gcgttccggc tgctgcatgc cgaaagtgtc
     5221 gcgcatcggg cggttaagcc acgggcccgg gtcgggcagc cggggccatg tgccttcatt
     5281 cagcccgccg agaatgatgt tctccgggtg gatcatgcgg gcttccagca agccaaggat
     5341 ggcaagccgc gtgacggtgt tcctggtgtc gcggaccgga agtctctgca gatgccggcg
     5401 gatggtggcg ctggcgcggg cgaaatcgca agcgggaagg cgcggagcct cccgaagcaa
     5461 ggtttcgata agttcctcaa gcgcgtcgga ctcaacaccg tcccagaacc catcgccgga
     5521 aatattttcc gccacggaaa tcaggccatc gagatgcatc gtgagcgagg tgacggctgg
     5581 gtgcgagagc ggacccagta tctccacaac gcgcgcaaga cagtcgcgca tctcccgaca
     5641 gtcgtcttcg ctgaggttgg caacgatggg atgcgggtgg gcttcgcctt tggagtttgc
     5701 gaaagcaagg tcgaacgagg gcagcaggcc ctccaggcca tggatcatcg gcgaagaccg
     5761 gaacagcgcc acttcgacct tccgtgccgc ggcgctgaag cccgcccgtt caaagccgaa
     5821 ccggctgaga tgatggctga acaggctgcg cagagaggag gccgagaagt tctcttcaac
     5881 cgcatccatg agaagcagca gaagcgacgg cgcactgaaa cggctgaggg gttcgccggc
     5941 ggaatcgtcc acccggatgt tccagcgcgc cagatttgct ttcacccgcc gcgccaggtc
     6001 ccggtccggc gtaaccaggg ccatgtcgcc cgaaggcttt tccagggact gccgcatcat
     6061 cacagagatg atcgcggcct cgtcattctt gtcgcgcgct tcggcaagcg tgacgttttc
     6121 aagcgcccgc ttgatcctgc tcccctggcc cgcaagcgcc gcctgccagt catcggaaac
     6181 atccgacggg cgcatgagtt cgctcgccag ccaggcccgg tctccgccag ccgtcccaag
     6241 cgtggccaca ttcttacgtt ccacgcccat gaaatcaatg agctgcttga tggcgtattg
     6301 cggatgctgg ggcgaaaccg ccgtccagct cttctcgtcc atctcctgat cgaggccggg
     6361 caggatcacc gccccgctct ccagcgccga aattgctttc agcaactcgc gggtggcggg
     6421 aatggtgccg gtggagcccg ccgcaatcat gggaccggcg ggcggattcg ccgcaagtct
     6481 tttggcttcc agatgaataa ggcggctgcg ccgctccttc gcccccataa gtttttccat
     6541 cacgagctga gccgggagtt tcctgcgcac caggtcgaac aggctgagaa tggcttcccg
     6601 gtgaaccgca aggtcaatat cgtaagcctc cggcaggcgc tcgaaggaaa cctcctcggt
     6661 ttccatgtca tcaatcagct cgccaagact ggctgcaagg gcttgcgcct gctgtggcgc
     6721 gtcagcgagt tcctgcgcta ggcgcagctg tggattttcg cgggcccaat catcaatcag
     6781 ggccataagc gcaaattcgc gcccgacatc gctgatcgcg tcttgcaagc cgctggccgg
     6841 gcgctgcgtt tccagcatat cttcgtcgag gtcgccaatg ggcctgatct gcggcagcac
     6901 gagtgcctgc ctgccgcttt ccatgagcag cttgctctga agggcgcgcg ccgcgcgccg
     6961 tgtcggcaca aggatggtcc aggcggcaag cgacggatgc ttttcaggtg agtcttccgc
     7021 ataagggaag ccctgcagaa ccgcttttgc cagaatgtcc aggaaaggcg cgtcgctggc
     7081 tatcgtgaag acgcgcggca gcctctcgcg cctcattcag attttggcaa gttcggcctc
     7141 ggccagagcg accgcggcag gggtgcccac gtgaaaccac tttccgctat gcgcgatgcc
     7201 atgcaggcgg ccctccgaaa ttgccttgtc ccacaagaca ttcatcgaga agttttttac
     7261 agcagtgccg gcaaaaaggc gaggatgcac gagatagccg ccgatatagg caagagcccc
     7321 cggtccctgc tggcgggtga gccgtccgtc acgccccaat tggaagtccc cccggccgtc
     7381 gtagccgatg gtgcggtcaa catggcaaag caggagaagg caatccattg ccgcatcatc
     7441 ccaggcggcc c
//
LOCUS       DAORVN010000010         7349 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_6041_length_7349_cov_4.038388, whole genome shotgun sequence.
ACCESSION   DAORVN010000010 DAORVN010000000
VERSION     DAORVN010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 7349)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7349)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7349
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6041_length_7349_cov_4.038388"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="PKV23_00435"
     CDS             <1..225
                     /locus_tag="PKV23_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008965755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HQX83925.1"
                     /translation="VLTGEHGVGIEKRDLMPVMFTEADLAQQQRLKCAFDDRGLLNPG
                     KVFPTLHACAELGRMHVRAGKLPFPDIPRF"
     gene            225..1385
                     /gene="glcE"
                     /locus_tag="PKV23_00440"
     CDS             225..1385
                     /gene="glcE"
                     /locus_tag="PKV23_00440"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011579710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HQX83926.1"
                     /translation="MTPAFKPRTVQELADYVAIGAKLEIVGRGTKRGLGRPLQAETAL
                     DMSGFTGISLYEPEELVLEAGAGTPLSEIERALAKNYQQLAFEPPDYSKLLGVHHRGT
                     LGGLMACGLSGPRRIKAGAARDHILGLAGVSGRGEIFKAGGRVVKNVTGFDVAKLMTG
                     SYGTLAALTSITVKVLPAAQTEETLVFSGLSDARAVQAMSLAMQSACEVSGAAHLPGI
                     PAKTLFRLEGIQPSVVYRRQKLASHLKIFGASDHLDEKESRAEWKAIRDVERLSADDA
                     RFVWKLSITPSKAAETVARISKQLDVQYFYDWAGGLVWLGVPRSNQAYAYIIRTCVQD
                     GHATLFRAEDEVRERIEVFQPQPPALSALTRRIKASFDPNGVYNHGRMYKDI"
     gene            1389..2687
                     /gene="glcF"
                     /locus_tag="PKV23_00445"
     CDS             1389..2687
                     /gene="glcF"
                     /locus_tag="PKV23_00445"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007819194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HQX83927.1"
                     /translation="MQTNFTAEQLKDPHTAEANKILRTCVHCGFCTATCPTYVLLGDE
                     LDSPRGRIYLIKNMLEKNEAAKAETVKHIDRCLSCLSCMTTCPSGVNYMHLIDQARVH
                     VEKTYRRPVFEMLLRTVLGFAMPRPPVFRLALSLARLARPFSKAFSALGLKRVEALLA
                     LVPGAPVAPEKLGAPGTYPAEGKRRGRVALLMGCVQSVIEPSINAATIRLLTRLGYEV
                     VVTGGETCCGSLTHHMGKEADAIWRARRNVHHWANARVDAVIITASGCGTTIKDYGFM
                     LRLDPVYAARAAVVSAKAKDIVEFLSGIELPAASQALRVTYHSACSMQHGQQMGTKAQ
                     TLLRNAGFTVQDIPEGHLCCGSAGTYNIMQPEIATQLRDRKIKNIKTTNPQVIATGNI
                     GCITQLATGLQVPILHTVELLDWVYGGPKPDKIKSLRGGA"
     gene            2691..4283
                     /gene="aceB"
                     /locus_tag="PKV23_00450"
     CDS             2691..4283
                     /gene="aceB"
                     /locus_tag="PKV23_00450"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013419536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HQX83928.1"
                     /translation="MARMPAGVTLRGKMLPEYRSILTPEALGFLADLHRTFDGERLRL
                     LELRAARARRFGKGELPDFLPETRAIREGSWRVAPIPADLQDRRVEITGPTDRKMVIN
                     ALNSGAKVFMTDFEDSNSPTWDNLVRGQINLRDRWSGAMDYRDPDSNKHYVLGKRPAV
                     LMVRPRGWHLPENHVRIDGQIMCGALFDFGLYFFHNAKAALRHGSGPYFYLPKMESHL
                     EARLWNSIFTSAQSALGLPLGTIKATVLIETLPAAFEMDEILYELREHMAGLNCGRWD
                     YIFSFIKTLATNRKFVLPDRSQVVMSKAFLASYSALLIKTCHNRAAFAMGGMAAQIPN
                     RKDPEANQIALDKVRADKEREARNGHDGTWVAHPDLVPVAMEVFNALMPTPNQLYVKR
                     EDVKIGQKELLEVHKGSKTEAGFRENIRVGVLYLEAWLRGRGAVPIYNLMEDAATAEI
                     SRSQIWQWLKFGATLDTGARVTRKFFDRCMREEMKRVKEEVGAEAYQEGRFKEAIGIF
                     KAMSTSKNFEAFLTLPAYKKII"
     gene            complement(4280..4552)
                     /locus_tag="PKV23_00455"
     CDS             complement(4280..4552)
                     /locus_tag="PKV23_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003521181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HQX83929.1"
                     /translation="MAALVRELEIINERGLHARASAKFVKCAEGFDANITVSRDGHSV
                     PATSIMGLMMLAAARGTSISVEASGPEAEAAMTALAALVESRFDED"
     gene            complement(4552..4950)
                     /locus_tag="PKV23_00460"
     CDS             complement(4552..4950)
                     /locus_tag="PKV23_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018065317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HQX83930.1"
                     /translation="MIGLVLVTHGRLADEFVYALEHIVGKQEMVAAVAIGPEDKMEVR
                     RGDIAAAVKSVDTGEGVIILTDMFGGTPSNLAISLLEEGRVEVVAGLNLPMLVKLVRV
                     RKDSNLHKCAAAAQDAGRKYINIASQILGD"
     gene            complement(5020..5517)
                     /locus_tag="PKV23_00465"
     CDS             complement(5020..5517)
                     /locus_tag="PKV23_00465"
                     /inference="COORDINATES: protein motif:HMM:NF019118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HQX83931.1"
                     /translation="MTESHLLLHGTCVSVGGAGVLILGAPGAGKSGLALRLIDEPGYG
                     ISGLLLRGELVADDQVVVTRVADRLMATAPASLRGKLEIRGLGIVSLATLPSAALALV
                     VKLQDHSAIERLPDGRSFDILGLALPLVEIDASMASSPARIRAALNWLKLPPAARETV
                     ALRHG"
     gene            complement(5517..7334)
                     /locus_tag="PKV23_00470"
     CDS             complement(5517..7334)
                     /locus_tag="PKV23_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006611448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stimulus-sensing domain-containing protein"
                     /protein_id="HQX83932.1"
                     /translation="MLDKTAAPEALERSESDEGQEVESFDLPAEGRRGLLHRFAPQRL
                     VSRIVLLNLLGLVILVTGILYFNQFRQGLIDARVQSLTTQAQIIAAAVAGSASADTGS
                     IVINPDSLEDYADPYAPDADQLDSLDFPINPETTGPVLRRLLANSTIRGRIIDPEGNL
                     VVDSRFLYGGGDIIQSDLPPRNAQPPNILIRWWNAAFDWFFAYDYPLQREYSLDNGKD
                     FPEVAAALNGAVVSVVRLDEKRDIIVLVSVPVQRFRAVLGALVLSTSGGEIDDVLRAE
                     RRVVLLTFGFVALVTILLSVFLAGTIAEPIRRLSAAAERVRRGINKRVEIPDFTARRD
                     EIGHLSGALRDMTGALYNRIDAIEAFAADVSHELKNPLTSLRSAVETLAMVKTDEQRA
                     RLVEIVKHDVRRLDRLITDISDASRLDAELARAETNPVDVARLLGAITTLANETRKEN
                     QAEIVLQVIPAGSNLDPLLAFVVLGHESRLSQVFRNLIDNARSFTVENTRIHVRVRRS
                     GRDVEIRVEDQGPGIRPDNLDRIFERFYTDRPEGSFGKNSGLGLSISKQIVEAHKGRI
                     WAENRYGRAPEGVEKPVLGARFVVRLPAIADDWTPPGKK"
BASE COUNT         1461 a         2212 c         2342 g         1334 t
ORIGIN      
        1 gtgctcaccg gcgagcacgg ggttggcatc gagaagcgcg atctcatgcc ggtcatgttc
       61 accgaggccg acctggcaca gcagcagcgc ctcaaatgcg ccttcgatga caggggccta
      121 ctcaatcccg ggaaagtgtt cccgacgctc cacgcctgtg ccgagctggg ccgcatgcat
      181 gtgagagcag gcaagctgcc gttcccagac attccgagat tctgatgacg cctgcattca
      241 aaccgcggac ggtgcaagaa cttgcggact atgttgcgat cggcgccaag cttgagattg
      301 taggacgcgg cacaaagcgc gggctcggcc ggcccctgca ggctgagacc gccctggaca
      361 tgtcgggttt cacaggcatc tcgctctatg agccggaaga actggtgctt gaggcgggtg
      421 cgggcacacc gctcagcgag attgaaagag cactggccaa gaactaccag caactggctt
      481 tcgagccgcc ggactattcg aaactgctgg gggtgcacca ccggggcaca ctgggcgggt
      541 tgatggcctg tgggctctcg gggccacggc gcatcaaggc aggagcggcg cgcgaccata
      601 ttctgggcct tgccggggtt tccgggcgcg gtgaaatctt caaggcgggt ggacgggtcg
      661 tcaagaatgt cacgggcttt gacgtggcca agctcatgac cgggtcctat ggcacgctgg
      721 cagccctcac cagcattacg gtcaaggtgc tgcccgccgc ccagacggaa gaaacgctgg
      781 ttttcagcgg cctcagcgac gccagggcgg tccaggccat gagccttgcg atgcagtcgg
      841 cctgcgaggt ttcaggggcg gcccatttac ccgggatacc ggccaaaacc ctgtttcgcc
      901 ttgagggcat ccagccctcg gtcgtctacc gccgccagaa gcttgccagc cacctgaaaa
      961 ttttcggggc gagcgatcat ctcgacgaaa aggaatcgcg cgccgagtgg aaggccatcc
     1021 gcgatgtcga gcggctgagc gccgacgacg cccgctttgt ctggaagctg tcgatcacgc
     1081 cttcgaaggc ggccgaaacc gtggcgcgga tttcaaagca gcttgatgtc cagtatttct
     1141 atgactgggc cggaggcctc gtatggctcg gggtgccgcg cagcaaccag gcctatgcct
     1201 atatcatccg cacctgtgtc caggacggcc atgccacgct tttccgggcc gaggacgagg
     1261 tgcgcgagcg gatcgaggtt ttccagcccc agcctccggc cctttcggcc ctcaccaggc
     1321 ggatcaaggc gtcctttgat cccaatggtg tgtacaacca cggccgcatg tataaagaca
     1381 tctgacccat gcagacaaat ttcacggcag aacagctgaa agatccgcac acggcggagg
     1441 ccaacaagat cctgcggact tgcgtgcatt gcggtttctg cacggccacc tgccccacct
     1501 atgtgctgct tggtgacgag ttggacagcc cgcgcgggcg catctacctc atcaagaaca
     1561 tgctggagaa gaatgaggcg gcaaaagcgg aaaccgtcaa acacatcgac cggtgcctgt
     1621 cgtgcctgtc gtgcatgacg acctgcccgt cgggcgtcaa ctacatgcat ctcatcgacc
     1681 aggctcgggt tcatgtggaa aaaacctacc ggcggcccgt gtttgaaatg ctgcttcgca
     1741 ccgtcctcgg gtttgccatg ccgcggcctc cggtgttccg gctggccctg tcgcttgcaa
     1801 gactggcccg gcccttctcg aaggcctttt ccgccttggg cctcaagcgg gtcgaggccc
     1861 tgctggcgct ggttcccggt gccccggtcg ccccggaaaa gttgggagcg cccggcactt
     1921 atccggcgga aggcaagagg cgcggccgcg tggcgctgct catgggctgc gtgcaaagcg
     1981 tgatcgagcc ttccatcaat gcggccacca tccgcctgct gacccggctg ggctatgagg
     2041 ttgtggttac gggtggagaa acctgctgcg gttcgctcac ccatcacatg ggcaaggagg
     2101 cggacgccat ctggcgggcg cggcgcaacg tgcatcattg ggccaatgca cgggtcgacg
     2161 cggtcatcat caccgcctcg ggctgcggca cgacaatcaa ggactatggc ttcatgctgc
     2221 gccttgatcc ggtttatgcg gcaagggcag cggtggtcag cgccaaggca aaggacattg
     2281 tcgagttcct ctccggcatc gagctgccag cagcttcgca agccctgcgg gtcacctacc
     2341 attcggcctg ttccatgcag catggccagc agatggggac caaggcgcaa acgctcttgc
     2401 gcaacgccgg cttcacggtt caggatattc cggaaggcca tctctgctgc ggctcggcgg
     2461 gcacctacaa tatcatgcag ccggaaatcg ccacgcagct ccgggacagg aagatcaaga
     2521 acatcaagac aaccaatccg caggtgatcg ccacgggcaa tatcggctgc atcacccagc
     2581 tcgcaacggg actgcaggtc cccattctcc acacggtcga acttcttgac tgggtttatg
     2641 gcgggccaaa gcccgacaag ataaaatctc tcagaggagg ggcttaagag atggccagaa
     2701 tgccagccgg ggtcacactt cgcggaaaaa tgctgccgga ataccgctcc attctcacgc
     2761 ctgaggccct tggcttcctg gcggacctgc accggacgtt cgacggcgaa aggctgcggc
     2821 tgctggagct acgcgccgcc cgcgccaggc gtttcggcaa gggtgaattg ccggatttcc
     2881 tgccggagac ccgcgccatc cgcgagggat catggcgcgt ggcgcccatc cccgccgatc
     2941 tgcaggaccg ccgggttgaa atcaccgggc ccaccgaccg caagatggtg atcaacgcgc
     3001 tgaactcggg cgccaaggtc ttcatgacgg acttcgagga ttcaaattcg ccgacctggg
     3061 acaatctcgt ccgcgggcag atcaatctgc gggaccgctg gagcggcgcc atggactacc
     3121 gcgacccgga ttcgaacaaa cattatgtcc tgggcaagcg gcccgccgtg ctcatggtgc
     3181 ggccgcgcgg ctggcacctg ccggagaacc atgtgcggat cgacggccag atcatgtgcg
     3241 gggcgctctt cgatttcggc ctgtattttt tccacaatgc caaggccgca ctccgccatg
     3301 gctccggccc ctatttctac ctgccgaaaa tggaaagcca tctggaagcg cggctgtgga
     3361 acagcatctt cacatcagcg caaagcgcac tcggcctgcc gcttggcacc atcaaggcca
     3421 ccgtgctgat cgagacgctg cctgccgcct ttgagatgga tgagatcctc tatgaactgc
     3481 gcgagcacat ggcggggctg aattgcgggc gctgggacta catcttttcg ttcatcaaga
     3541 cgcttgcgac aaacaggaag tttgtcttgc cggaccgctc gcaggtggtg atgtcaaagg
     3601 cgttcctcgc cagctattcg gcactgctga tcaagacctg ccacaaccgc gcggccttcg
     3661 cgatgggcgg catggcagca cagattccca accgcaagga tccggaagca aaccagatcg
     3721 cgctggacaa ggtgcgggcc gacaaggagc gggaggcgcg caatggccat gacggcacct
     3781 gggtggcaca tcccgacctg gtgccggttg ccatggaagt gttcaacgcc ctgatgccga
     3841 cgccgaacca gctttatgtg aagcgtgaag atgtgaagat cggccagaag gagcttctgg
     3901 aggttcacaa gggcagcaag accgaagcgg gattccgcga aaatatccgg gtgggggttc
     3961 tttatcttga agcctggctg cgggggcgcg gtgcggtgcc gatctacaat ctgatggagg
     4021 atgcggcgac cgctgaaatc tcgcggtcgc aaatctggca atggctgaag tttggcgcaa
     4081 cgcttgatac gggcgccagg gtcacccgca aattcttcga cagatgcatg cgcgaggaaa
     4141 tgaaacgggt gaaggaggaa gtgggggcgg aagcctacca ggagggccgc ttcaaggaag
     4201 caatcggcat tttcaaggcc atgtccactt cgaagaattt cgaggcgttc ctgacgctgc
     4261 cggcctacaa gaagatcatc tagtcctcgt cgaacctgga ttccaccagg gcggcaaggg
     4321 ccgtcatggc ggcctcggcc tcggggcccg aggcctctac gctgatcgag gttcccctgg
     4381 cggcagccag catcatgagc cccatgatgg aggttgccgg aaccgaatgg ccgtcacggc
     4441 tcacggtgat gttggcgtca aatccctcgg cgcatttcac gaatttcgcc gaggcgcggg
     4501 cgtgcaggcc gcgctcattg atgatttcca actccctgac cagcgccgcc atcaatcccc
     4561 caggatctgg ctggcgatgt tgatgtattt gcggcccgca tcctgggcgg cggcggcgca
     4621 tttgtgaagg ttggagtcct ttcgcacccg caccagtttc accagcatgg gcagattcaa
     4681 tccggccacg acttcgacgc ggccctcttc gaggagcgag atcgccaggt ttgatggcgt
     4741 gccgccaaac atatcggtca ggatgatcac gccttcgccg gtatcgacgg acttcaccgc
     4801 tgcggcgata tcgccgcggc gcacttccat tttatcctcg ggaccgatgg ccacggcggc
     4861 aaccatctcc tgtttgccga caatatgctc cagggcatag acaaattcat cggcaagacg
     4921 cccatgggtt acgagaacga ggcctatcat ctgaaagctg cgaaatcaca cattgccgga
     4981 tcatgcctca tttggcgttt taagccgcgc acattgccct tatccatgcc gcagtgcaac
     5041 agtttcacgg gcagcagggg gcagtttcag ccagttcagc gcggcgcgaa tgcgggccgg
     5101 gcttgaagcc atgcttgcat caatttcaac cagcggaaga gcgaggccca gaatgtcaaa
     5161 actccggcca tcggggaggc gttcaatggc tgaatggtcc tgaagcttca ccacaagtgc
     5221 cagggcggcg gaaggaagcg ttgccaggga gacaatgccg aggccgcgga tttccagttt
     5281 gccacgaagc gaggcaggag ccgttgccat cagcctgtcc gccacgcggg tgacgacgac
     5341 ctgatcatcg gccacaagct cgccgcgcag gagaaggccg gatattccat agcccggctc
     5401 atcgatgagc ctgagcgcca ggccggattt tccggctccg ggagcgccca gaatcagcac
     5461 gcccgcgccc ccgacactga cgcaggttcc atgcaggagg agatgggact ctgtcatcat
     5521 ttctttccgg gcggcgtcca gtcatcggca atggcgggaa ggcgcaccac gaatctcgcg
     5581 cctagcaccg gcttttccac gccctccgga gcccggccat agcggttctc ggcccagatc
     5641 cggcccttgt gggcctcgac tatctgcttg gagatggaca ggccgaggcc ggaattcttg
     5701 ccgaatgagc cttccgggcg gtcggtgtaa aaacgctcaa agatacgatc aagattgtcg
     5761 ggccggatgc cgggcccttg gtcttccacg cggatctcga catcgcggcc ggagcgccgg
     5821 acccggacgt ggatccgggt attttcaacg gtgaaggagc gggcattgtc gatgagattg
     5881 cggaagacct ggctcagccg cgactcgtgg ccaagaacga caaaggcgag caacggatca
     5941 aggttgcttc ccgccggaat cacctgcagg acaatttccg cctggttctc cttgcgggtt
     6001 tcattggcaa gggtcgtgat cgcgccgaga agtcgggcca cgtcaaccgg atttgtctca
     6061 gcgcgggcca gttccgcatc gaggcgcgag gcatcggaga tatcggtgat gaggcggtcg
     6121 agccggcgca cgtcatgctt gacgatctcg acaagcctgg cgcgctgctc atcggttttc
     6181 accatggcca gggtctcaac ggcgctgcgc agcgaggtga gtggattctt gagttcgtgg
     6241 cttacatcgg cggcaaaggc ctctatggca tcaatgcggt tgtaaagcgc gccggtcatg
     6301 tcacgcagcg cccccgagag atggccgatc tcgtcgcgcc gggcggtgaa gtcgggaatc
     6361 tcaacgcgct tgttgatgcc gcggcgtacc cgctcggcgg cggccgaaag gcggcgaatg
     6421 ggctcggcga tggtgcctgc caggaatacc gagagaagaa tggtcacgag ggccacgaag
     6481 ccgaaggtga ggagcacgac gcggcgctcg gcacgcagaa cgtcgtcgat ttcgccgccc
     6541 gaagtggaga gcaccagggc gccgaggacc gcacggaaac gctgcaccgg aaccgacacc
     6601 agaacgatga tgtcgcgctt ttcatcgaga cgcaccacgc tcaccacggc gccgttgagg
     6661 gcagccgcca cttccgggaa atccttgccg ttgtcgaggc tgtattcgcg ctgcagcgga
     6721 tagtcatagg cgaagaacca gtcaaaagcc gcgttccacc agcgaatgag gatgttcggc
     6781 ggctgggcgt tgcggggcgg aagatcagac tggatgatgt cgccgccgcc gtagaggaac
     6841 ctgctgtcaa cgacgagatt gccctcgggg tcgattatcc gtccacggat ggttgagttg
     6901 gccagcaggc ggcgcagcac cgggccggtg gtttccggat tgatggggaa atcaaggctg
     6961 tcgagctggt cggcatcggg cgcataggga tcggcgtagt cttccagcga atccgggtta
     7021 atgacgatgg aacccgtatc ggcgctggcc gatcccgcca cggcagcggc tatgatctgg
     7081 gcctgggtgg tgagactctg cacgcgggcg tcaatcagcc cctggcggaa ctggttgaag
     7141 tagagaatgc cggtgaccag gatgacgagg ccaagaagat tgaggagaac gatgcggctg
     7201 accagccgct ggggggcaaa gcggtgcaag aggccgcggc ggccttcggc gggcaggtcg
     7261 aaactttcaa cctcctggcc ctcgtcgctt tcgctgcgtt caagggcctc aggtgcggca
     7321 gtcttatcga gcatttcgca ccctcacac
//
LOCUS       DAORVN010000011         7076 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_6459_length_7076_cov_3.727247, whole genome shotgun sequence.
ACCESSION   DAORVN010000011 DAORVN010000000
VERSION     DAORVN010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 7076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7076
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6459_length_7076_cov_3.727247"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..795)
                     /locus_tag="PKV23_00475"
     CDS             complement(<1..795)
                     /locus_tag="PKV23_00475"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HQX83933.1"
                     /translation="MAQTQQAQAVVAAIFLLAIGQLFAIGLGWARGLTLQDAIRSLAR
                     ERRAADEKYNKSFVTAKLFENEIADLKRRMGLSEKEIQDEMAAMRIQFQELADRYAAD
                     SANARLQQQNAADFPITLQGAPAREHFNFLLEPVIDLTTNRTAHYRVRFSITAANGIE
                     IDYSKLTMNADRGELRPTLDLHVINQAIPLLRRLRAKHPAMRMFIPMGAATLVSEHTI
                     AMVTQMLSDADNVAQGVVFELTHDSLAGLNEAGISGLAAIARMGATL"
     gene            complement(971..2329)
                     /locus_tag="PKV23_00480"
     CDS             complement(971..2329)
                     /locus_tag="PKV23_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011995192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HQX83934.1"
                     /translation="MNKPQIHSDAKTWEASPNNLEPFWMGFTPNRAFKQRPRMIARAK
                     DMHYYDMAGRAILDGSAGLWCSNAGHCRDPIVAAIQKSAAELDFAPTFQYGHPKVFEL
                     ASRLATMAPGDLNYAFFCNSGSEAADSALKIALAYQRVKGQASRTRLIGRERGYHGVN
                     FGGISVGGMVGNRKMFGTMLAGVDHIPHTYNRKEQAYTKGEPDYGMHYADELERLVAL
                     HDASTIAAVIVEPMAGSTGVLPPPKGYLKRLREICDKHGILLIFDEVITGFGRLGYAF
                     AAERFGVIPDMITFAKGVTSGTVPMGGVMVRQGIYDAFMNGPEHAIELFHGYTYSGHP
                     IAAAAGVATMDLYRDEKLFENARKLEGVWADAAMSLKGNPLIEDIRTLGLVAAFDLKA
                     VDGSPGLRAYKAMENAFHDFGFLIRITADTIALSPPLMITENQIDEIFSDKMPKVLKS
                     VA"
     gene            2487..3215
                     /locus_tag="PKV23_00485"
     CDS             2487..3215
                     /locus_tag="PKV23_00485"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HQX83935.1"
                     /translation="MSSFSNIIAGGLIGLGVACGGFLAGESLVKSRLGFRTVTVKGLS
                     EREVKANLGFWPVRFVATGPTLEEARSSLEVSERAVKAFLMSHGFTEQDMQVQNIQVE
                     DRLTGYNNQGVAPEIRFVLTEDILVKSSEVQKLADASRMIGDLLKSGVVFSSDAYNAG
                     PSFIFTDVNELKGDMLTEATKRAREAADKFAQESGARVGDIQNANQGIIEINPAVAIP
                     NDRPERQIDKKVRVVTTITYFLSD"
     gene            complement(3346..3606)
                     /locus_tag="PKV23_00490"
     CDS             complement(3346..3606)
                     /locus_tag="PKV23_00490"
                     /inference="COORDINATES: protein motif:HMM:NF018294.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HQX83936.1"
                     /translation="MREYVAHEARSRLNAFSFSTLRQLVRNWRCRKQLLKLHGLDDYQ
                     LKDIGLTRGQLRHLAGLPLSVDPVWEADRIRLLASRQKPERS"
     gene            3736..5124
                     /locus_tag="PKV23_00495"
     CDS             3736..5124
                     /locus_tag="PKV23_00495"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HQX83937.1"
                     /translation="MTIWTPQLSQDKPIYLAIADAMAGDIMSGKLNVGDKLPPHRDLA
                     WRLKVTVGTVTRAYKEAEIRGLLSGEVGRGSYVREVSGSTALPSRAEVATQIADLSHA
                     APPPVYSADEFDAALAHVMREPSRLALLDYMPPEGHVHYRAMGARWLARSGIDISEAN
                     VIVTSGAHLGILACLSVLAQPGESVMAENLNYASLGSLVLGLNLKPVPLAMNETGLDL
                     EDLERAAKTGESKILYLVPTLQNPTTFTMPREKRDAIVEIARRYNLTIVEDDIFRLLD
                     DRVQPPTFYSLAPERTYHITSLSKTLAPGLRIGFVATPPGRAQFLRLRQRASGARVTG
                     LTAEVARYWLETDIAEHLMRRVIGEMGARRSIFREVFSRHAFKCEPGAPFGWLTLPGH
                     WSPLRFASKALSHNVRIIPGPAFSFGPQARDHGVRICFGAPATGQDLREALLKIRDLM
                     GGDPEDDFTPVA"
     gene            complement(5121..>7076)
                     /locus_tag="PKV23_00500"
     CDS             complement(5121..>7076)
                     /locus_tag="PKV23_00500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX83938.1"
                     /translation="PPLKAQTLNTTDFQIEWKVVNRFRLFNDASFFRLHENAWRQYLI
                     HVNEQQIPDELKSLLINSASVLGSEHVLNDRYIAFSRLERSKFDWRGWAAQGQGRLCW
                     NPKSRLHDLCGDVDGYVNPKAHEIEISLRPLRKNPLISEYNCEWRVGDAPVQIAPCDG
                     TVTASLPYPGGADISVAVAGEQPIALKAKVRDLLIVGMGDSFASGEGNPDMPAEFSET
                     QRHNNLYPRRKNNDASGSALWTDELCHRSLYGHQLRAALQIAIENTQSAVTFLGYACS
                     GAGIEAGILGPQEYVESVSTTSAATSDAQAAPVTRFLSGNSNDAQIRWLLRELCMVKP
                     KENDGLWVCPDKQFRRPVDYLMLSIGGNDVGFRNVVAWATLRSGTSATLAKFLGATVS
                     AKDFARNVKDTLPGAYARLAKAIERAVPLYSEEPVFDATRVILSAYPDMLVDETGAVC
                     PAGGKEEDEEDRYPANQSLDAFSSWLATTSGRLNAVHEQLAELDRRMRDLSGDHGWTY
                     AGRVYNDQVFRGHGFCAQRSKFANDPAEVLMIPCWGKADRPTQTCQSSWSGKAREWRP
                     YSPVTQNYPYALRQRWVRTFNDAYMAVNQKVLDKAGKVDEKASSQTFSETTGAMHPNA
                     EGQAAMADAILMDIREEVAKLLSDELY"
BASE COUNT         1436 a         2009 c         2219 g         1412 t
ORIGIN      
        1 gagggtcgcg cccatccggg caatggccgc gaggccggaa atgccggcct cgttaagtcc
       61 ggcaagggag tcatgggtga gttcaaagac cactccttgc gccacgttgt cggcatccga
      121 cagcatctgg gtgaccatgg caatggtgtg ctcgctcacc agggtggcag cccccatggg
      181 aatgaacatg cgcatggcgg ggtgcttggc acgcaggcgg cgcagcagcg ggatggcctg
      241 attgatgaca tggagatcaa gcgtggggcg cagttcacca cggtcggcat tcatcgtcag
      301 tttcgaatag tcgatttcga tgccgttggc agcggtgatg ctgaagcgca cccggtaatg
      361 ggcggtgcgg ttggtggtca ggtcaatgac cggttcgaga aggaaattga aatgctcgcg
      421 cgcaggcgct ccctgcagcg taatcgggaa atcggcggcg ttctgctgct gcaggcgggc
      481 attggcggaa tcggccgcat agcgatcagc cagttcctga aactggatgc gcatggcggc
      541 catttcatcc tggatttcct tttcggaaag gcccatgcgg cgcttcaggt cggcgatttc
      601 attttcaaag agcttggcgg tcacaaagga cttgttgtat ttttcgtccg cggcgcggcg
      661 ctcacgggca aggctgcgaa tggcatcctg cagggtgagg ccgcgggccc agcccaagcc
      721 aatggcgaac aactgcccga tggcgagaag aaatatggcg gccacaacgg cctgggcctg
      781 ctgggtttgc gccaccaata caaaaatccc gcatgcaatt gcaaagcagg aaaacactgc
      841 aagtaccaga acgcttggcc aaccacgcac gagcttatta ccgcatattg tccccgttaa
      901 cctgaatcaa tagggccaaa aacgaaatga gtctatggct ccatgatcgg ggaattggcg
      961 acgtggttaa tcaggcaacg gatttcagaa ccttcggcat cttgtcgctg aaaatttcat
     1021 caatctggtt ttcggtgatc atcagcggcg gcgacagggc aatggtatcg gcggtgatgc
     1081 ggatgaggaa tccaaaatca tggaaggcat tttccatggc tttgtaggcg cgaagcccgg
     1141 gtgagccgtc aaccgctttc aggtcaaagg cggccacgag gccaagggtc ctgatatcct
     1201 cgatcagcgg attgcccttc agcgacatgg cggcatcggc ccagacgcct tccagcttcc
     1261 tggcgttctc gaagagcttc tcatcgcggt agaggtccat ggtggcgaca ccggcggcgg
     1321 cagcaatggg gtgccccgaa taggtgtagc catggaagag ttcgatggcg tgctcgggtc
     1381 cgttcatgaa agcatcatag atgccctggc gcaccatgac gcctcccatg ggcacggtgc
     1441 cggaggtcac acccttggca aaggtgatca tgtcggggat gacgccgaaa cgctcggcgg
     1501 caaaggcata gcccaaacgg ccaaagccgg tgatgacttc atcgaatatc agcaggatgc
     1561 catgcttgtc gcagatttcg cgcaggcgct tgagatagcc cttgggcggc ggcagcacgc
     1621 cggttgagcc cgccatgggc tcgacaatga cggcggcaat ggtagaggca tcgtgcaggg
     1681 ccacgaggcg ttccagctca tcggcataat gcatgccgta gtcgggctcg cccttggtgt
     1741 aggcctgttc cttgcggtta taggtgtggg gaatgtggtc aacgccggcc agcatggtgc
     1801 cgaacatctt gcggttgccc accatgccgc ccacggaaat gccgccgaaa ttcacgccgt
     1861 gatagccgcg ctcgcggcca atcaggcggg tgcggctggc ctggcccttg acgcgctgat
     1921 aggccagcgc aattttcagg gcggagtcgg ctgcttccga accggagttg cagaagaagg
     1981 catagttcaa atcgccgggc gccatggtgg caaggcgcga ggcgagctcg aacaccttgg
     2041 gatggccata ctggaaggtt ggcgcgaaat cgagctcggc ggcggatttc tggatcgccg
     2101 ccacgatggg atcgcggcaa tggccggcgt ttgaacacca caggccggcg ctgccatcga
     2161 gaatggcgcg gcccgccatg tcgtaataat gcatgtcctt ggcccgggcg atcatccgcg
     2221 gacgctgctt gaaggcgcgg ttgggggtga agcccatcca gaacggttcc agattgttgg
     2281 gcgaggcttc ccaggttttg gcgtcactat ggatctgcgg cttgttcatt gaaaagactc
     2341 cagctggcat tttcggccaa gatagcagaa ccgtggccgc aagggaaaca accagactta
     2401 atcattgccc cactccagat aagacaaaaa agcaccacaa tgggcagaca gtttgcaagc
     2461 agaggggcga cactcaagga acttccatgt catcattttc gaatatcatc gccggcgggc
     2521 tgatcggcct cggcgtggcc tgcggcggct ttcttgccgg tgaatcactg gtcaagagcc
     2581 gcctgggatt ccgcaccgtc acggtcaagg gcctttcgga gcgcgaagtg aaagccaatc
     2641 tcggcttctg gccggtgcgg tttgtggcca ccggccctac gcttgaagag gcgcgctctt
     2701 cccttgaagt ttcagaaagg gcggtgaagg ccttcctcat gtcgcatggc ttcaccgagc
     2761 aggacatgca ggtgcagaat atccaggttg aagaccggct gactggttat aacaaccagg
     2821 gggtcgcccc tgaaatccgc ttcgttctca ccgaggacat tctggtcaaa tcatcggaag
     2881 tgcagaagct cgccgatgcc tcgcggatga tcggcgacct gctgaaatcc ggcgtggtgt
     2941 tttcctcgga tgcctataat gccgggcctt ccttcatttt caccgacgtc aacgaactca
     3001 agggtgacat gctcacagag gccaccaagc gggcgcgcga ggcggctgac aagtttgcac
     3061 aggagtcggg cgccagggtt ggcgatatcc agaacgccaa ccagggcatc atcgaaatca
     3121 acccggcggt ggcaattccc aatgaccggc cggagcggca gatcgacaag aaggtgcggg
     3181 tggtgacgac gatcacctat ttcctgtcgg attgagttcc aaggtcatca tcaacaaatt
     3241 attgtcaccc cggcgaaagc cggggcccag acttcgaaca cctgtgactc ttgaaagtct
     3301 gggtcccggc tttcgccggg atgacagatt gttgggatgg acctcttaac tccgctccgg
     3361 cttctggcgg gaagcgagga gcctgatgcg gtcggcttcc caaacgggat caaccgagag
     3421 cggcaggccg gcaagatggc gcagctggcc acgggtcagg ccgatatctt tcaactggta
     3481 gtcatcgagg ccatgcaatt tgagaagttg cttacggcag cgccaattgc ggacgagctg
     3541 gcgcagggtt gaaaagctga atgcgttcaa ccgggaccgg gcttcgtggg ctacatattc
     3601 gcgcatggca atctcctcgt aaatcctctc taaattgtat ctattgtacc tatattgtca
     3661 tgatttctgt tgaaaaatca cttgatgtat agatacaatg agattattgt atgtgtcaaa
     3721 gaggatattg tatccatgac aatttggacg ccgcagctca gccaggacaa accgatttat
     3781 ctcgcgattg ccgatgccat ggccggcgac atcatgtccg gcaagctgaa tgtcggcgat
     3841 aaactgccgc cgcaccggga tctggcgtgg cgcctcaagg tgacggttgg aacggtcacc
     3901 cgggcctaca aggaagcgga gatccgcgga ctgctttccg gcgaggtggg ccggggaagc
     3961 tatgtgcgcg aagtgagcgg gtcaacggcc ctgccatcgc gcgccgaagt ggcaacgcaa
     4021 attgccgatc tcagccatgc cgcgccgccg ccggtttatt ccgccgatga atttgatgcg
     4081 gcgctggccc atgtgatgcg cgagccttcg cggctggcgc tgcttgacta catgccgccg
     4141 gaaggccacg tgcattaccg cgccatgggc gccaggtggc tggcgcgcag cggcatcgat
     4201 atttcagagg caaacgtcat tgtcacctcg ggcgcccatc tcggcatcct ggcctgcctc
     4261 tcggtgctgg cgcaacccgg cgaatccgtc atggcggaga atctcaatta cgcttcactc
     4321 gggtccctcg ttcttggcct aaacctgaag cccgtgccgc ttgcgatgaa tgagacgggg
     4381 cttgaccttg aagacctcga acgcgccgcc aagaccgggg aatcaaaaat tctctatctt
     4441 gttcccaccc tgcagaatcc cacaactttc accatgccgc gcgagaagcg tgacgccatc
     4501 gttgagatcg cccggcgcta caatctcacc atcgtcgagg acgatatttt ccgcctgctt
     4561 gacgaccggg tgcagccacc gacgttttac agcctcgcac cggagcgcac ctatcatatt
     4621 accagcctgt cgaaaacgct ggcgccgggc ctgcgcatcg gtttcgtggc aacgccgccg
     4681 gggcgggcgc aattcctgcg cctgcggcaa cgcgcgtcag gagcccgggt cacggggctt
     4741 accgcagagg tcgcacgcta ttggctggag acggacatcg ccgaacacct gatgcggcgc
     4801 gtcatcggag agatgggggc acggcgcagc attttccgcg aagtgttttc caggcacgcc
     4861 ttcaagtgcg agccgggcgc gccctttggc tggctgacgc ttcccggcca ctggagcccg
     4921 ctgcgttttg cttcgaaggc cctgtcgcac aatgtacgga tcattccggg gccggcattt
     4981 tccttcggac cgcaagccag ggatcatgga gtccggattt gctttggcgc gccggctacc
     5041 ggccaggacc tccgggaagc gctgctcaaa atccgcgacc tgatgggcgg ggacccggaa
     5101 gacgatttca cgccggtggc ctaatacagt tcatcgctca acagcttggc cacctcttcg
     5161 cggatatcca tgaggatggc gtcggccatg gcggcctggc cttcggcatt gggatgcatg
     5221 gcgccggtgg tttcggaaaa ggtctggctc gaagccttct cgtcaacctt gcccgccttg
     5281 tccaatacct tctggttcac cgccatgtag gcatcattga aggtgcgcac ccagcgctgg
     5341 cgcagcgcat agggataatt ctgggtgacc gggctgtagg gccgccattc tctggccttg
     5401 cccgaccagg aggactggca ggtttgggtg ggcctgtcgg cttttcccca gcagggaatc
     5461 atcaacactt cggccgggtc attggcaaat ttgctgcgct gggcgcaaaa accgtggccg
     5521 cggaaaacct ggtcattgta aaccctgccg gcataggtcc agccatggtc gccgctcaga
     5581 tcgcgcatgc gccggtccag ctccgcaagc tgctcatgaa cggcattcag ccggccgctg
     5641 gttgtcgcaa gccaggatga aaacgcatcg agggactggt tggcgggata gcggtcttcc
     5701 tcatcttctt ccttgccgcc cgccgggcag accgcgccgg tttcatcgac cagcatgtcg
     5761 ggataggccg agaggatcac gcgggtggca tcgaagacgg gctcctcgga atagagcggc
     5821 acggcgcgct caatggcttt ggcgaggcgg gcgtaggcgc cgggaagcgt gtccttcacg
     5881 ttcctggcaa aatcctttgc cgaaacggtg gcgccgagga atttcgccag ggtggccgag
     5941 gttccgctgc gcagggttgc ccaggcaacc acgttcctga agccaacgtc attgccgccg
     6001 atagagagca tgagatagtc aacgggccgg cgaaactgct tatcgggaca gacccagagg
     6061 ccgtcattct ccttcggctt caccatgcag agttcgcgca acagccagcg gatctgcgca
     6121 tcattggaat taccggacag aaagcgcgtc actggcgctg cctgcgcgtc agaggtcgcg
     6181 gcactggtgg tgctgacact ctcgacatat tcctgggggc caagaatgcc cgcttcaatg
     6241 ccggcgccgg aacaggcgta gcccaaaaag gtcaccgccg actgggtgtt ctcaatggcg
     6301 atctgcaggg cggcgcgcag ctgatggccg tagagcgagc ggtggcagag ttcatcagtc
     6361 catagggcgg agccgctggc atcattattc ttgcgccggg gataaagatt attgtggcgc
     6421 tgggtttcgc tgaactcggc cggcatgtcg ggattgcctt cgccggaagc aaagctgtcg
     6481 cccatgccga caatcagcag gtcacgcact ttcgctttca gggcaatggg ctgttcgccg
     6541 gccacggcaa cggaaatatc agcgccgccc ggatagggca gcgaggcggt gaccgtgccg
     6601 tcgcagggcg cgatttggac gggagcgtcg ccaacgcgcc attcacaatt atattcgctg
     6661 atcaggggat ttttcctgag ggggcgaagg ctgatttcaa tctcatgggc cttgggattc
     6721 acatagccat cgacatcgcc gcagagatca tgcaggcggc tcttcggatt ccagcacagc
     6781 cggccctgac cctgtgccgc ccagccgcgc cagtcaaact tcgaacgctc gaggcgcgaa
     6841 aaggcaatgt agcggtcatt gagcacatgc tcgctgccca gcaccgaggc gctgttgatg
     6901 agcagggatt ttagctcatc ggggatctgc tgctcgttca cgtggatgag atattgccgc
     6961 caggcgtttt cgtggagcct gaagaagctg gcgtcattga aaagccggaa acggttaacg
     7021 accttccatt cgatttggaa atccgtggtg ttgagggttt gggccttaag aggcgg
//
LOCUS       DAORVN010000012         7006 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_6582_length_7006_cov_3.496763, whole genome shotgun sequence.
ACCESSION   DAORVN010000012 DAORVN010000000
VERSION     DAORVN010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 7006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7006
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6582_length_7006_cov_3.496763"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="PKV23_00505"
     CDS             complement(<1..237)
                     /locus_tag="PKV23_00505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX83939.1"
                     /translation="MSNETTPPPVPVGEGFLRKFLNKFSASRDKGLREDLESVIDTHQ
                     AQNPQDGPGQETRSMMRNLIKFSDLRVDDVMVPRA"
     gene            complement(234..689)
                     /gene="ybeY"
                     /locus_tag="PKV23_00510"
     CDS             complement(234..689)
                     /gene="ybeY"
                     /locus_tag="PKV23_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009449328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HQX83940.1"
                     /translation="MNLDISIDDREWRVVPNLRGLARTAVLAVLPDDDVAIGLLFTSD
                     ARIAEMNGQWRGKAGPTNVLSFPVPAETPVPAGEPRPLGDIALAYGVISREALAQKKP
                     FAHHVTHLIVHGVLHLLGYDHENDDEAGAMEAREIMILSELGMGNPYTP"
     gene            complement(686..1711)
                     /locus_tag="PKV23_00515"
     CDS             complement(686..1711)
                     /locus_tag="PKV23_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018094011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HQX83941.1"
                     /translation="MNSQASAAQLATLKSSPSADDHDTLTLAFDDNRLLSLLFGEHDE
                     HIALIEHRMGVAITPRGNRVSIRGMGPARDGAKNVLSQLYQRARRGLEVNRGEVEGAI
                     RMNQSVHDKEVKEGTVRTRRKLVMPRTPMQKNYVEAIRKNELVFGIGPAGTGKTYLAV
                     ACAAEALMNGEVDRIILSRPAVEAGERLGFLPGDMKDKVDPYLRPLYDALYDMMPAAL
                     VTKGLAENQIEIAPLAFMRGRTLASAFIILHEAQNTSPQQMKMFLTRLGEGSRMVITG
                     DPSQVDLPSGASSGLDDALRVLKGVQGTEQIRFSTSDIVRHALVGRIVAAYDKLGAKV
                     KSLSQPR"
     gene            complement(1749..3098)
                     /gene="miaB"
                     /locus_tag="PKV23_00520"
     CDS             complement(1749..3098)
                     /gene="miaB"
                     /locus_tag="PKV23_00520"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014327072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HQX83942.1"
                     /translation="MTVTPKSVYVKTYGCQMNVYDSERMRDTLKGMGYAETPVPDLAD
                     LVIFNTCHIREHAAEKVYSELGRMKRLKKAKAESGGEMLVAVAGCVAQAEGAEIIRRA
                     KTVDLVFGPQTYHRLPDLIARRLKTGDAIVETDFPAEDKFVALEQQNANRVLNRNVSA
                     FLTVQEGCDKFCSFCVVPYTRGAEFSRPPSQIEREARRLADAGVREITLLGQNVNAYH
                     GEGLSLPGLIAKLSHISGIKRLRYTTSHPRDMDEALIEAHASNPKLMPFLHLPVQSGS
                     DRILKAMNRGHGTAAYMDTVAKVRAARPDMALSGDFIVGFPGETDADFEDTLTLIEEV
                     GYASAYCFKYSPRPGTPAAEDPDQVPDEVVAERLARIQALIHAQQTAFNRSCVGRSLP
                     VLLEKPGREPSQLMGRSPYLQPVHVNGQKAQIGAVLEVKIDSVGTNSLSGHIELVEA"
     gene            complement(3108..3884)
                     /locus_tag="PKV23_00525"
     CDS             complement(3108..3884)
                     /locus_tag="PKV23_00525"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HQX83943.1"
                     /translation="MSRLRATLVLLTFAGVTLPLMPVQQLFIWFWPRMARRFPMHYHR
                     LVLRILGVRVRRDGETLRQGPAIFAANHVSWLDIVILSSLAPVSFIARHDINTWPFFG
                     ALARLQRTVFVDRARRHTSRASRDEMRERLAAGDMLVLFAEGTSGDGTRVLPFKSTFF
                     SAVENSNVAVQPLSVVYTGHRALPMPRHLRPLYAWYGDMEMPPHLWAAMAQGPIEVNI
                     VCHAPLSTADHGDRKTLAKKAEEAVRRGLVDTLHGRAKMS"
     gene            complement(3886..4980)
                     /gene="tsaB"
                     /locus_tag="PKV23_00530"
     CDS             complement(3886..4980)
                     /gene="tsaB"
                     /locus_tag="PKV23_00530"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HQX83944.1"
                     /translation="MLTLALDTSMAACSVCVYDAGPGLVLASRHDFMDRGQAEALAPM
                     VQETMASAGVAFKGLARIAVTIGPGTFTGVRIGLAMARGLGVALSIPVTGINSLAAIA
                     CNETAGDLPTVVAADARANEIYFASYDASGRELIAPVIVKLAEAHKLIPTYPVKMLGT
                     GADLLLEKTDKQHVRSDAGDLPVARNFVKLAASIPPSPVPPEPLYLRPPDVKPQAMKI
                     SFSTMGQAAAKLLAELHGESFATRWSETDFHGMLAVPGTQAILVSSQNNPMGFVLFRT
                     AVDEAEVLTICTRPEFRRKGHGKLLMQHLESVLKSGGVKSLFIEVAVSNHAALALYAS
                     SGFEKAGARKNYYARSDGKREDALIMRKGI"
     gene            complement(5028..5456)
                     /locus_tag="PKV23_00535"
     CDS             complement(5028..5456)
                     /locus_tag="PKV23_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018234964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0048029 - monosaccharide binding [Evidence
                     IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RbsD/FucU domain-containing protein"
                     /protein_id="HQX83945.1"
                     /translation="MLKGIDPVLGPELLAVLRGMGHGDEIAVVDGNYPAETDARRLVR
                     MDGHDAPRIVEAILSVMPVDAFVPEAVFRPAAGGDAKRLEPVFADFEEILKKYEPKQK
                     ISVLVGSDFYNRVKACYAIVASGERRLYGNLILRKGVIHP"
     gene            complement(5457..6680)
                     /locus_tag="PKV23_00540"
     CDS             complement(5457..6680)
                     /locus_tag="PKV23_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010970632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HQX83946.1"
                     /translation="MDWAIGGQVLGKGPTLARGTTQNGVRLYNERLALSLIRRHGSLP
                     KAEIARLTGLSAQTVSVIVRQLETDQLLIKETPQRGKVGQPLVPFSLNPDGAFSIGLK
                     IGRRSGDLILLDLTGKVRRKVHQPYVFPTPQKIMDFVAVELEGLLSGLSPEQKSRISG
                     LGIATPFELWNWEEEIGASHEVLEAWRDFDIAAEIAKLGGWPVHFSNDATAACAAELL
                     FGRGNRFNSYAYFFVGYFVGGGIVINGHIFPGKAGYAGALGSFPMMTPDGSPQQLTHN
                     ASLYVLENRLQAEGRDPMLLARSPEDWGEIGPALDVWLEQTARSLAFAAVATMSIIDF
                     DAIIIDGAMPAKVRTDLVQKTRTALSRQDLRGLPPFSIEEGSIGADARAMGAASLSLF
                     ANYIIDRDVLFKEAF"
BASE COUNT         1384 a         2120 c         2077 g         1425 t
ORIGIN      
        1 ggcccgcggc accatcacgt cgtcgacccg caggtcggaa aacttgatca ggttgcgcat
       61 catcgacctg gtttcttggc cgggaccgtc ctgcggattc tgcgcctggt gggtgtcgat
      121 gacgctttca aggtcctcgc gcaggccctt gtcccgggag gcggaaaact tgttcaggaa
      181 tttccggaga aaaccctccc cgacggggac gggcggcggt gtcgtttcgt tactcatggt
      241 gtatagggat ttcccattcc cagttcggaa agtatcatga tttcgcgggc ctccatggcc
      301 ccggcttcgt catcgttttc atggtcgtat cctagcagat gcaatacgcc atgtacgatc
      361 aaatgcgtga catggtgagc gaagggcttt ttctgcgcca gcgcctcccg ggaaatgacg
      421 ccgtaagcca gcgcaatgtc gccaaggggc cggggttcgc ccgccggtac cggggtttcg
      481 gcgggaaccg gaaatgacag gacattggtg ggccctgcct tgccgcgcca ctgcccgttc
      541 atctcggcaa tccgcgcatc cgaggtaaag agcaggccga ttgccacatc atcatccggc
      601 agcacggcca aaaccgcagt tcgggcaagc ccgcggagat tgggaaccac acgccattcc
      661 cggtcgtcaa tactgatgtc gaggttcatc gcggttggga gagacttttg accttggctc
      721 ccagcttgtc ataggcggca acaatccgtc cgaccagcgc gtgcctgacg atgtcgctgg
      781 tggagaacct gatttgttcc gtgccctgaa cccctttcag gacccgcagg gcgtcgtcca
      841 ggccggaact tgcgccagac ggcaggtcaa cctggctggg gtcgccggtg atcaccatgc
      901 ggctgccttc gccgagcctg gtcaggaaca tcttcatctg ctgcggcgag gtgttctgcg
      961 cctcgtggag gatgatgaag gcggacgcca gggtacggcc gcgcatgaag gcgagcggcg
     1021 cgatttcaat ctggttctcg gccaggccct tggtaaccag cgccgccggc atcatgtcgt
     1081 aaagcgcatc atagagcggg cgcagatagg gatcgacctt gtccttcatg tcaccgggca
     1141 ggaagcccag gcgctcgccc gcttcaacgg cgggacgcga caggatgatc cggtccactt
     1201 cgccattcat cagggcttcg gccgcacagg cgaccgccag ataggtcttg cccgtgcccg
     1261 ccgggccgat gccgaacacc aattcgttct tgcgtatggc ttccacataa tttttctgca
     1321 tgggcgtgcg cggcatcacc agcttgcgcc gcgtgcgcac cgtgccttcc ttgacctcct
     1381 tgtcatgaac cgactgattc atgcggattg ccccttcaac ttcgccgcgg ttgacttcaa
     1441 ggccgcggcg cgcccgctgg taaagttgtg atagcacgtt cttggcgccg tcgcgcgccg
     1501 ggcccatgcc gcggatcgac acgcggttgc cgcgcggcgt gatggccacg cccatgcggt
     1561 gctcgatcag ggcgatgtgt tcgtcgtgtt cgccgaataa aagtgagagc aggcggttgt
     1621 cgtcaaaagc gagggtgagt gtgtcgtggt cgtccgcact ggggctggac ttgagcgttg
     1681 cgagctgggc ggcactggct tgcgaattca actgtggctt tctcctgttg ttgcttccga
     1741 cctcagaatc atgcttcgac caattcaata tggcccgaaa ggctgtttgt gccaacacta
     1801 tctattttaa cctccagaac agccccaatt tgggcctttt ggccattgac gtgtaccggc
     1861 tgcaggtatg gtgaacgccc catgagctga cttggttccc ggcccggttt ttcaaggaga
     1921 accggcaagg atctgcccac gcaggagcgg ttgaaggcgg tttgctgggc atggatcagg
     1981 gcctggatgc gggccagccg ctcggccacc acctcatcgg gcacctgatc gggatcctcg
     2041 gcggcaggcg tcccgggtcg cggcgaatac ttgaagcaat aggcgctggc gtaacccacc
     2101 tcttcaatga gagtcagggt atcttcaaaa tccgcatccg tttcgccggg gaagcccacg
     2161 atgaaatcac ccgacagtgc catgtcgggc cgtgccgccc gaacctttgc gacagtgtcc
     2221 atgtaggcgg ccgtaccgtg gccccggttc atcgctttca ggatgcggtc gctgccgctt
     2281 tgaaccggca gatgcaggaa gggcatgagc ttcgggttgc tggcatgggc ctcgatcaac
     2341 gcttcgtcca tatccctcgg atggcttgtg gtataacgca agcgcttgat accggaaatg
     2401 tgtgacagct ttgcgatgag ccccggtagc gacaggccct cgccgtgata ggcattgacg
     2461 ttctgtccca gcagtgtgat ctcgcgtacc cccgcatcgg caagcctgcg ggcttcccgt
     2521 tcgatctgtg acggcgggcg cgagaattcg gcgccgcgcg tatagggcac cacgcagaag
     2581 ctgcagaact tgtcgcaacc ctcctgcacc gtgagaaagg ccgagacatt ccggttgagg
     2641 acccggttag cattctgctg ttccagcgcc acgaacttgt cttccgccgg aaaatccgtt
     2701 tcgacaatcg cgtctccggt tttgagccgc cgcgcgatga ggtccggaag gcggtgatag
     2761 gtctgcgggc caaagaccag gtcaacggtc ttggcccggc ggatgatttc cgcgccttcc
     2821 gcctgggcca cgcagcccgc cactgctacc agcatctcgc cgccagactc cgccttggct
     2881 ttcttgaggc gcttcatccg gccaagttcc gaatagactt tttccgccgc atgctcacgg
     2941 atatggcagg tattgaagat caccagatcg gccagatcgg gaaccggcgt ttccgcatag
     3001 cccatgccct tcagcgtatc gcgcatgcgc tcgctgtcat agacattcat ctggcagcca
     3061 taggttttaa cgtaaaccga ctttggggtc acagtcatgg ccgtgtgcta gctcattttc
     3121 gcccgcccgt gaagcgtgtc caccaggccg cgcctgaccg cctcttccgc ctttttggcc
     3181 agggttttcc ggtcgccatg atcggccgtg gagagcggcg catggcagac gatgttgacc
     3241 tcgattggcc cctgggccat ggcggcccag agatgcggcg gcatctccat gtcgccatac
     3301 caggcataga gcggccgcag atggcgcggc atcggcaggg cccggtgacc tgtatagaca
     3361 acgctcaggg gttgaactgc gacattggaa ttttctacag ccgaaaagaa agttgatttg
     3421 aagggaagta cccgcgtgcc gtcgcccgag gtgccttccg caaacagcac cagcatgtcg
     3481 ccggctgcga gccgctcgcg catttcatcg cgtgaagcgc ggctcgtgtg gcgtcgcgcg
     3541 cgatcaacaa acacggtgcg ctgcagccgc gccagcgccc cgaagaaggg ccaggtattt
     3601 atatcatgcc tggcaatgaa ggacaccggc gccagcgaac tgaggatcac gatatcaagc
     3661 caggacacat gatttgccgc aaatatcgcc ggcccctgcc gcagcgtttc gccgtcgcgc
     3721 ctcacccgga ccccgaggat gcgaaggacg aggcggtggt aatgcatggg aaagcgccgg
     3781 gccatgcgcg gccagaacca gatgaagagc tgctgcaccg gcatgagcgg aagcgtgacg
     3841 ccggcaaagg tcagcagcac cagcgtggcg cggagccggc tcatgtcata tacccttgcg
     3901 catgatgaga gcatcttcgc gtttgccatc gctgcgcgcg taatagtttt tccgggcgcc
     3961 ggctttttca aatccgctgg atgcatagag cgcgagggcc gcatgatttg aaacggcgac
     4021 ttcaatgaac agggacttca cgccgccgct cttgagcaca ctttcgaggt gctgcatcag
     4081 cagcttgcca tgccccttcc ggcgaaattc cgggcgggtg cagattgtca atacctccgc
     4141 ttcatcaacg gctgtgcgaa acagcacgaa gcccatcggg ttgttctggc ttgaaacaag
     4201 aatggcctgc gtgccgggaa ctgccagcat accgtggaaa tccgtttcac tccaccgcgt
     4261 cgcgaaactt tcaccatgaa gttccgccag cagttttgcc gccgcttgcc ccatcgtgct
     4321 gaacgaaatt ttcatggcct gcggcttcac atccggcggt cgcaagtaaa gcggttccgg
     4381 cggcacaggg gatggcggaa tgctcgcagc gagtttcaca aaattcctgg caaccggcag
     4441 gtcaccggca tcactacgca catgttgctt gtcagtcttt tccagcagca ggtctgcccc
     4501 tgttccaagc atcttcaccg gataagttgg tatgagtttg tgtgcttcag ccagtttgac
     4561 aatgaccggc gcgatcaact cacgtcccga tgcgtcataa gacgcgaaat aaatttcgtt
     4621 ggcccgcgca tctgctgcaa ccacggtggg aagatcgcct gccgtttcat tgcaggcgat
     4681 tgctgccaga gaattgatgc cggtgacggg aatgctgagc gcaacgccca ggccccgcgc
     4741 catggcaagc ccgatgcgga ccccggtgaa ggtgccgggg cctatcgtga ccgcgatgcg
     4801 ggcaaggcct ttgaacgcaa cgcccgccga tgccatggtt tcctgcacca tgggcgccag
     4861 ggcttccgcc tggccccggt ccatgaagtc atggcgcgaa gccagcacga gcccggggcc
     4921 agcgtcataa acgcagaccg agcaggccgc cattgaagtg tccagggcaa gagtaagcat
     4981 tacgctgctt gataggccgc aatgatgaaa agatgcaagt tcacgcgcta gggatgaata
     5041 acgcccttgc gcaggatcag gttgccgtaa agccttcgtt cgcccgaggc cacgatggcg
     5101 tagcaggcct tcacccggtt atagaaatcc gagcccacca gtaccgaaat tttttgcttt
     5161 ggctcgtatt ttttaaggat ctcttcaaag tccgcaaaca ccggctccag gcgcttggcg
     5221 tctccgccgg ccgccggccg gaacacggct tcggggacga atgcgtccac cggcatcacc
     5281 gagaggatgg cttcgacaat ccgcggcgca tcgtggccat ccatgcgcac caatctcctg
     5341 gcatccgttt cagccggata attgccgtcc accacggcaa tctcgtcgcc gtgccccatg
     5401 ccgcgcagca ccgccagcag ctccggcccc agcaccggat caatgccctt gagcatttag
     5461 aacgcctcct tgaacagcac gtcacggtcg atgatgtaat tggcgaagag cgaaaggctg
     5521 gcggccccca tggcgcgcgc gtcggccccg atgctgccct cttcgatgct gaatggcggc
     5581 agcccgcgca gatcctgtcg cgaaagggcg gtacgggttt tttgcaccaa gtcggtccgc
     5641 accttggcgg gcatcgcacc gtcaatgatg atcgcatcaa aatcgatgat cgacatggtg
     5701 gccacggcgg cgaaggcgag gctcctggcg gtttgctcga gccagacatc aagagctggg
     5761 ccaatctcac cccaatcctc gggcgaacgc gcaagcagca tgggatctcg cccctccgcc
     5821 tgcaggcggt tctccagaac atagagactg gcgttgtggg tcagttgctg cgggctgccg
     5881 tcgggtgtca tcatcggaaa tgaaccaagg gcccccgcat agccagcctt ccccggaaaa
     5941 atatggccgt tgatgacaat cccgccgccg acaaaatagc cgacgaagaa ataagcatag
     6001 ctgttgaacc ggtttcccct gccaaacagc agctcggccg cacaggccgc cgttgcgtca
     6061 ttgctgaaat gcaccggcca gccgcccagc ttggcaattt ctgcggcaat atcgaagtcg
     6121 cgccaggcct ccagaacctc atgcgaggcg cctatctctt cttcccagtt ccagagctcg
     6181 aagggtgttg caatgccgag gccgctgatc cggctcttct gttccggcga aaggcccgac
     6241 aataggccct caagttcgac cgcaacaaaa tccatgatct tttgcggcgt gggaaaaaca
     6301 tagggctggt gcaccttgcg ccgcactttc ccggtgaggt ccagcaggat gagatcgccg
     6361 gagcgccgcc cgatcttgag gccgatggaa aaggccccgt ccgggttgag tgagaagggc
     6421 acaagcggct ggccaacctt gccgcgctgc ggcgtttcct tgatcaggag ctggtcggtt
     6481 tccagttgcc ggacgatgac tgaaaccgtt tgtgccgaaa gccccgtcag ccgggcgatc
     6541 tcggccttgg gcaggctgcc atgccggcgg atgagcgaaa gggccaggcg ttcattgtaa
     6601 aggcgcacgc cgttttgcgt agttccacgg gccagcgtgg ggcctttgcc caatacctgc
     6661 ccgccaatcg cccaatccat cgtcacgccc gtccacctgt ttggaattta tgcaaatggc
     6721 ccgcaggttg ctgaaatttt gactttcccg taggggcaga accttgtcaa tcaataaatc
     6781 acgcggattt atttattgac tcttggcaag gaattcgctc aattccgcaa atggaacagg
     6841 actcaccggc atgatcctga ctccgcttcg agaagtaact ggacttaaat cgtcgacgaa
     6901 gttagactaa attttacata agtaataaga cttatgtatg taattgtact taactaagcc
     6961 caaaaatggg cgcgtaccat gggagaatca tagtgaaaac aattct
//
LOCUS       DAORVN010000013         6901 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_6768_length_6901_cov_3.053024, whole genome shotgun sequence.
ACCESSION   DAORVN010000013 DAORVN010000000
VERSION     DAORVN010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 6901)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6901)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6901
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6768_length_6901_cov_3.053024"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            113..1639
                     /locus_tag="PKV23_00545"
     CDS             113..1639
                     /locus_tag="PKV23_00545"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQX83947.1"
                     /translation="MQFVYFLIAAFNLVAIFCGLYLSHLVIGVYQDNVSESAFFDRQL
                     SASWVIADSAADAQSEYVKVLSTKAPAQAQSMFKSKSYEFRQEISRLRAQIPLHTDEK
                     PANKALALLRRMDTAMTEIEKHAARIDLLVEAGDMESAQAAVTRIVERYSSLRFSIKD
                     LNQLIGRMKLNAVEEANRTVTKLRNYEFYIGVLMAVVICAVAFYGQFMGRLLKRKFVE
                     LEKAHNELASSHEQALSFSQEIQNVNNEMGTLNRQLTDNITKLREAQDEALRKGKMAQ
                     LGNLTATVAHELRNPLSTVRTSTYLLARKIKDKGLGVEPQLQRINNGVIRCDNIITQL
                     LDFSRTKAIKAESTVLDVWLERLVQAEAQKLPDLVSIECYFGLGEKLVELEPGRFERA
                     IINLLTNASEALVGKGDDPKARYTETPVITVRTRLTARGAEISVADNGPGISADNLTK
                     IREPLFTTKNFGTGLGISAVEQILEQHGGGLDIDSVEGKGATFTLWLPLHESSGEQAA
                     "
     gene            1636..2361
                     /locus_tag="PKV23_00550"
     CDS             1636..2361
                     /locus_tag="PKV23_00550"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQX83948.1"
                     /translation="MKRSLRILVVDDDADNANSLGELFELEGHRPLVVYDGEAAIAAY
                     LKEDFDIAFMDVMMPGKNGVESFLEIKKLKPLAKVYMMTGYSVEQLLQQAMAGGAMGV
                     FSKPVNLYQVLDSISGLGSSGVVLVAEDDPDFGPTLAQLINRNGYRCELVRNGRDAVS
                     RVLAGGVDVLVLDLRMPLISGIEVYNTLKKQGRSVPTIIITGTASEHSKDLALFDDIE
                     LTGILNKPFDPLELLDRLEKLVA"
     gene            2384..3946
                     /locus_tag="PKV23_00555"
     CDS             2384..3946
                     /locus_tag="PKV23_00555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HQX83949.1"
                     /translation="MTTQRKTPASRINDTDVLLQILDLLPTSLFVKDENLNFVFSNEA
                     HCKVIGSEAEKLLGLSDADFYPKDQAEKFLAADKSVLNSPEAREFLELATSTDGSTIY
                     SLTRKARVVTSDGATYLIGSNTDVTEMKRNEIELEAKEARYRALAETVPVGIWHLHEN
                     GKTIYVNPFLLTLLGMTEGEFLATAPKDFLTAASEKGVMEMIGSTARFETDLMARGKV
                     AARVLVVSSGWLNNAGVAGRSVMVTFVDISKVSSLQTVNDEITRLNAELSDKIKMLRA
                     TQEEMVKAGRMAQLGQLTATVAHELRNPLAAVRTSAFVLDRKIKGKGMGVEQQLQRIE
                     NGVVRCDDIISQLLDFARTRPVQRKLLDLDTWLAKVVEEEAAKLPAVVTIECMLGLDG
                     LVAAIDPERMSRGLINLISNASEALVGKGDELHGDASFVPAINIESGRTVRGIEISVT
                     DNGPGITAENLLRIKEPLFTTKSFGTGLGLSAVEKILEQHGGGLEIKSKPGEGARFTA
                     WFPLIETLEEAA"
     gene            3948..4310
                     /locus_tag="PKV23_00560"
     CDS             3948..4310
                     /locus_tag="PKV23_00560"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQX83950.1"
                     /translation="MARSLSILVLDDNIENADSLAELLSMDDHKVKVAYSGQEAIDAY
                     RATPFDLGFFDVMMPGKNGVDSFLEIRQERPDAKVYFMTGYSAEDLLKKAMEGGALGF
                     FGKPMDLPLVLDVCQQAA"
     gene            complement(4315..5526)
                     /locus_tag="PKV23_00565"
     CDS             complement(4315..5526)
                     /locus_tag="PKV23_00565"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017964507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006102 - isocitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HQX83951.1"
                     /translation="MTKIKVANPVVELDGDEMTRIIWDLIKKKLIHPYLDVDLHYYDL
                     SVENRDATNDQVTIDAANAIRKHGVGVKCATITPDEGRVQEFKLKQMWKSPNGTIRNI
                     LGGVIFREPIICKNVPRLVPGWTKPIIIGRHAFGDQYRATDFTFPGKGTLTIKFVGDD
                     GKVIEKEVFKAPGEGVAMAMYNLDDSIKEFARASLNYGLNRNYPVYLSTKNTILKVYD
                     GRFKDIFQAIYDTEFKAEYEKRGLTYEHRLIDDMVASAMKWTGGYIWACKNYDGDVQS
                     DLVAQGYGSLGLMTSVLMTPDGKTVEAEAAHGTVTRHYRLHQQGKQTSTNSAASIFAW
                     TGGLKHRAKLDSNAALAKFASTLEAVTIATIEEGSMTKDLAVLVGPEQTWLSTEGFLD
                     AVDKNLQKAAA"
     gene            complement(5638..6582)
                     /locus_tag="PKV23_00570"
     CDS             complement(5638..6582)
                     /locus_tag="PKV23_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018098209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HQX83952.1"
                     /translation="MAKYRQNLPQLSNQLFLTDGGIETTFIYHNGIDLPYFAVFDQLK
                     SEKGTQALRDYYSRHAAIARANGLGFILESPTWRASADWGKKLGYTPSALADINRQSI
                     AFLETLRNEFETAQSPMPISGCIGPRGDGYDPGRIMSEAEAEDYHAGQAQVFAKTAAD
                     MVTAITMTNINEAVGVTRAARATAMPVVISFTVETDGRLPSGELLRQAIEAVDAKTGN
                     APAYFMVNCAHPTHFDDTLVKADWSKRIRGIRANASKCSHAELDKATELDAGNPLEFG
                     QQYRAIRDRLSHINVLGGCCGTDHRHVEQIALACLKAA"
BASE COUNT         1547 a         1807 c         2034 g         1513 t
ORIGIN      
        1 catttgttta ccataattgg agcaccgtgg cttatcaatg ctttgagaag cgggcaggag
       61 cacggggttg gagaaggcac cacaaagaag cggccaaaag tcgtggccga gagtgcaatt
      121 tgtctatttc ctgattgccg cattcaatct cgttgcgatt ttctgcggcc tgtatttgag
      181 ccatctggtg atcggcgttt atcaggacaa cgtcagcgaa tcggcctttt ttgaccgcca
      241 gctgtcggct tcatgggtga ttgccgattc tgctgcagat gcgcagtcgg aatatgtgaa
      301 ggtgctcagc accaaagccc cggcgcaggc gcaatcgatg ttcaaatcca aatcttacga
      361 attccgccag gaaataagcc gcctgcgcgc gcagattccg ctgcataccg acgaaaagcc
      421 cgcaaataag gctctggccc tgctgcggcg gatggacacc gccatgaccg agatagaaaa
      481 gcatgcggca agaattgacc tgctagtgga agcaggcgat atggagtcag cccaagcggc
      541 cgtgacccgc atcgtcgaac gttattcctc gctcagattc agcatcaagg acctcaacca
      601 gctgatcgga aggatgaagc tgaacgcggt cgaagaggca aacaggaccg tcaccaaact
      661 gcgaaactat gagttctata ttggcgttct gatggcagtt gtcatttgcg ccgttgcctt
      721 ctacgggcag ttcatgggac gtctgctgaa acgaaaattc gttgagcttg agaaggccca
      781 caatgaactt gcctcttcgc acgagcaagc gctctcgttt tcgcaggaga ttcagaacgt
      841 caacaacgag atgggcacac tcaaccggca gctcaccgac aatatcacca aactgcggga
      901 ggcccaggat gaggcccttc gcaagggcaa gatggcccag ctcggcaact tgaccgcaac
      961 tgttgcccat gagctccgca atcccctcag cacggtcagg acctcgacgt atcttttggc
     1021 gcgaaagatc aaggacaagg gtctgggagt tgaaccacag ctccagcgga tcaataacgg
     1081 cgtaatccgt tgcgacaaca tcatcacgca gcttttggat ttttcacgga ccaaggcgat
     1141 aaaggccgag tcaacagtgc tcgatgtctg gcttgagagg ctggtgcagg ccgaagcgca
     1201 aaagctgccg gatctggttt ccatagaatg ctatttcgga ctgggagaaa agctggttga
     1261 gcttgagccc gggcggtttg aacgggccat catcaacctg ctcaccaatg cctcggaagc
     1321 actggtgggc aaaggcgacg accccaaggc aagatatacg gaaaccccag tcattacagt
     1381 caggacacgg ctgacggcac gcggtgcgga aatcagcgtt gccgataacg ggccgggcat
     1441 ttccgccgac aatctcacga aaatccgcga accgctgttt acaaccaaaa acttcggcac
     1501 cggccttgga atatcagcgg tggagcagat tctggagcag catggcggag gccttgacat
     1561 agattccgtg gaaggcaagg gcgccacttt taccctttgg ctgcctctcc atgaaagcag
     1621 cggagaacaa gcagcatgaa acggtcactg cgtatcctgg ttgttgacga tgacgcggac
     1681 aacgcaaact cgctgggcga gctctttgaa ctggagggcc accggccgct tgtcgtctat
     1741 gatggcgaag cagcaatcgc ggcctatctc aaggaggatt ttgacatcgc attcatggat
     1801 gtgatgatgc cgggaaagaa tggcgtggaa agcttcctcg aaatcaagaa gctgaaaccc
     1861 ctggccaagg tctatatgat gaccggttac agcgtggagc agcttctcca acaggccatg
     1921 gccggcgggg ccatgggggt gttctccaag ccggtcaatc tttaccaggt gctggactcg
     1981 atttccggcc tcggttcatc gggggttgtc cttgtggccg aggatgatcc tgatttcggc
     2041 cccacactgg cccaactgat caaccgcaat ggctaccggt gcgaactggt ccgcaatggc
     2101 cgtgacgcgg tatcgcgggt cctggcaggc ggcgtggacg tactcgtgct cgacctcagg
     2161 atgccgctga tcagcggcat tgaagtctac aacacgctca agaagcaggg ccgcagcgtt
     2221 ccaaccataa tcatcacggg aacggcttcg gagcacagca aggacctggc gctttttgac
     2281 gatatcgagc tgaccgggat tctgaacaag ccgtttgatc ctttggagct tctggaccgc
     2341 cttgagaaac tggttgcttg agccgcttgg ggaagaaact gcaatgacta ctcagagaaa
     2401 gaccccggcg tcacgcatca acgatactga cgtcttgttg cagatcctgg atcttctccc
     2461 gacgtcgctg ttcgtgaaag acgagaacct caactttgtg ttttcaaatg aagcacactg
     2521 caaggtcatc ggatcggagg cggaaaagct tctgggtctt tccgacgccg atttctatcc
     2581 aaaggatcag gcggagaagt ttctggcggc tgacaaatcg gtgctcaatt cgccggaggc
     2641 cagggaattc ctcgagcttg ccaccagcac tgacggttca accatttatt ccctcacgcg
     2701 taaagcccgc gtcgtaacct cggatggcgc cacatacctt attggctcca ataccgatgt
     2761 tacggagatg aaaaggaacg aaatcgagct ggaagccaag gaggcccggt atcgcgctct
     2821 tgcagaaact gttcctgttg gaatctggca cctgcatgaa aatggaaaaa caatttatgt
     2881 aaacccattt ctcctcacgc ttcttggcat gacggaaggc gagtttcttg caactgcccc
     2941 gaaggacttt ctgaccgcag cctcggaaaa aggcgttatg gaaatgattg gatcgactgc
     3001 gcgtttcgaa accgatctga tggcccgggg caaggttgcc gcaagggtgt tggttgtttc
     3061 gtccgggtgg ctgaataacg ccggagttgc ggggcggtcg gtgatggtga cattcgtgga
     3121 catatcgaaa gtcagcagtc tgcaaacggt aaacgatgaa atcacgcgtc tcaatgcgga
     3181 actctcggac aaaatcaaaa tgttgagagc cacgcaggaa gaaatggtga aagcgggccg
     3241 catggcgcag cttggacaat tgaccgctac ggttgcccat gaactgcgca atccgctggc
     3301 agcggtccgg acttcggcat tcgttcttga ccgaaagata aaggggaagg gaatgggtgt
     3361 tgagcagcag ctgcagcgaa tagagaacgg ggttgtccgc tgcgatgaca tcatctccca
     3421 gctcctggat tttgcccgca cccgcccggt tcagcgtaag cttcttgatc ttgatacctg
     3481 gctcgccaag gtcgttgagg aggaagcggc caagctgccg gcggtggtga ccatcgagtg
     3541 catgctgggg ctggacggat tggttgcggc gatcgacccg gagcggatga gccgcggcct
     3601 gatcaacctg atcagcaatg cctcggaggc tctggtcggg aaaggcgatg aactgcatgg
     3661 cgatgccagt tttgtcccag cgatcaacat tgagtcgggc cggaccgtgc gcggcattga
     3721 aatcagcgtt actgacaatg ggcctggcat aaccgccgag aatcttttga gaatcaagga
     3781 gccgctgttt acgaccaaga gttttggcac tggccttggc ctgtccgcgg tcgagaagat
     3841 tctggagcag cacggcggcg gacttgagat caaatccaaa ccaggcgaag gcgcacgctt
     3901 tacagcctgg tttccactca ttgagacact agaggaggcg gcctgacatg gcgcgatcac
     3961 tatcaatact tgtacttgac gacaatatag aaaacgctga ttcgcttgcc gaactgctgt
     4021 cgatggacga ccacaaggta aaggtcgcct atagcggcca ggaggccata gacgcttatc
     4081 gcgccacgcc ttttgatctg ggtttctttg acgtgatgat gccaggaaag aatggagtgg
     4141 acagctttct ggaaattcgc caggagcgac ccgacgccaa ggtatacttc atgacaggct
     4201 acagcgcaga agacctgctg aagaaggcca tggagggcgg cgcgctgggg tttttcggca
     4261 agcccatgga cttgccgctt gtgttggatg tgtgccagca ggcagcctag ccgatcaggc
     4321 agcggccttc tggagattct tgtcgacggc atcgagaaag ccttcggtgg agagccatgt
     4381 ttgctctggt cccacgagga ctgccagatc cttcgtcatg cttccttctt cgatggttgc
     4441 aattgtcacc gcctccaaag tgctggcgaa tttcgccagc gcagcgttgc tgtcgagttt
     4501 cgcgcggtgc ttcaggccgc cggtccaggc gaagatcgac gcggcggagt tggtggaggt
     4561 ctgcttgccc tgctggtgca ggcggtaatg ccgtgtcacg gttccgtggg ccgcttcggc
     4621 ctccacggtc ttgccgtccg gtgtcatcaa gacggacgtc atgagcccaa gtgagccata
     4681 accctgggcc acgagatcgg actgtacgtc gccatcatag ttcttgcagg cccagatata
     4741 cccgccggtc cacttcatgg cggatgccac catgtcgtca atcaggcgat gctcataggt
     4801 aaggccgcgc ttttcgtact cggccttgaa ttcggtgtca tagatcgcct ggaaaatatc
     4861 cttaaagcgc ccatcataga ccttgagaat ggtattcttg gttgacaggt agacgggata
     4921 gttgcggttc aggccatagt tcaggctggc gcgggcaaat tccttgatgc tgtcatcaag
     4981 attgtacatg gccatggcca cgccttcgcc cggcgccttg aaaacttcct tttcaatgac
     5041 cttgccatca tcgcccacga acttgatggt gagcgtgccc ttgcctggaa aggtgaaatc
     5101 ggtggcgcgg tactggtcgc cgaaggcatg gcggccgatg attatgggct tggtccagcc
     5161 aggcacaagg cgcggcacat tcttgcagat gatgggttcg cggaaaatga cgcctcccag
     5221 gatgttgcgg atggtaccgt tgggcgactt ccacatctgc ttcagcttga attcctggac
     5281 ccggccctca tcgggggtga tggtggcaca tttcacgccg acgccgtgtt tcctgatcgc
     5341 attggccgcg tcaatcgtca cctgatcatt ggtggcatcg cggttttcga cggagagatc
     5401 ataatagtgc aaatcaacat cgagataggg atgaatcagc tttttcttga tcaggtccca
     5461 gatgatgcgg gtcatttcat cgccatcgag ttcaaccacg gggttggcga cttttatctt
     5521 ggtcatttgt ggaacctcat cattttgggc tggaattcgg cgttgctata gcaggcgcat
     5581 gatgaggggg caaactggaa gaaaaggggg ccaaacggcc ccccatggat atccgcgtca
     5641 tgctgcctta aggcaggcga gggcgatttg ctcgacgtgc cggtggtcgg tgccgcaaca
     5701 gccgcccagc acattgatgt ggctgaggcg atcccggatg gcccggtatt gctggccgaa
     5761 ttccagtgga tttcccgcat caagctcggt tgccttgtca agttcggcat ggctgcattt
     5821 ggaggcattg gcgcgaatgc cgcggatgcg tttgctccaa tcagccttga ccaatgtgtc
     5881 gtcgaaatgg gtgggatggg cacaattgac catgaaatag gccggcgcat ttcccgtctt
     5941 ggcatcgacg gcttcgatgg cctggcgcag caactcgccg gagggcaggc gcccatcggt
     6001 ttccacggtg aacgaaatta ccactggcat tgccgtcgcc ctggcggcgc gcgtcacgcc
     6061 aaccgcttca ttgatgttgg tcatcgtgat ggcggtcacc atgtcagctg ccgtctttgc
     6121 aaagacttga gcctgacctg cgtgatagtc ctcggcttca gcctcgctca tgatgcggcc
     6181 gggatcgtag ccatcgccgc gcgggccaat gcagccgctg atcggcatgg ggctttgggc
     6241 cgtctcgaac tcattgcgga gcgtttcgag gaaagcgatg gactggcggt tgatgtcagc
     6301 cagggcggac ggggtgtacc caagtttttt gccccagtcg gcgctcgccc gccaggtggg
     6361 cgattcaagg atgaacccca ggccattggc gcgggcgatt gcggcatgcc ggctgtaata
     6421 atcgcgcagg gcctgcgtgc ccttttcgga tttaagctga tcaaacaccg cgaaataggg
     6481 caggtcgatg ccattatgat agatgaaagt tgtttcaatg ccgccgtcag tcagaaacaa
     6541 ttgattgctt aactgcggca gattctgccg gtattttgcc attttattct ccattgttga
     6601 aagatacgga gcgcaaggcg cttcgaatgg cggcaaaatg attgagagcc tccaggaaat
     6661 ctacaaacct tatggtcaag gcttttcaaa aaatggaaac ttctatatag atcatgctgt
     6721 taggcagttc cggccggacc cttaacggac ccgcagggaa gtttgccaag ctctaactgt
     6781 acttgcggta aagtggatat gacttcaacg gtcctgaatc gtgctggcag caaacgtgaa
     6841 cgtatcctcg aggtggcgga agaggccatc ctcgcgaagg gatttgcggc tacgtcaatt
     6901 g
//
LOCUS       DAORVN010000014         6709 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_7115_length_6709_cov_3.188759, whole genome shotgun sequence.
ACCESSION   DAORVN010000014 DAORVN010000000
VERSION     DAORVN010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 6709)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6709)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6709
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7115_length_6709_cov_3.188759"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            255..1877
                     /locus_tag="PKV23_00575"
     CDS             255..1877
                     /locus_tag="PKV23_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015316589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HQX83953.1"
                     /translation="MTETFDHIIVGGGSSGCVAAARLASEGGAKVLLLEAGYSNRHPL
                     LDMPPGIFKMINGSKFMRYHHTTPQAHLDGRVHDIPQGNVLGGGSSVNAQVYIRGRPA
                     DYDEWNDLLRGNNDRIGWGWDDVLPHFTRMEGNNRFMNGRHGTDGPLQVSDPGHINEF
                     SRWFVQAVQGLGEPFNSDFNGPLQRGVGFYQFTNRNGKRSSAAYAFLSAQENNPNLTV
                     RLQCEAQRVVIEAGRAVGVIYKDKSGALRQARCEGEVIVASGALVTPKLLMLSGIGPA
                     SHLAAHGIACLVDSPGVGQNLIDHPEVPITAIANGPFGYYKQGEGWRMLRNGLQFKLF
                     GTGPVTSAGVEAGAYVNPRNPEGLPSIQAFCVPIVYLDRDALDLVKDTFGITVTTVVV
                     KPRSRGEVRLASADPSEMPMVSPNLLKDPADMQEMVDGQRFFLRAFEQKPLADRLRQI
                     VIPLPGDRSDAALQAHCRRFVKTNYHPVGTAKFGADSDDMAVLDARLRVRGVEGLRVC
                     DLSAMPNIVAGNTNAPAMMLGDRCADLVMGNL"
     gene            1990..2970
                     /locus_tag="PKV23_00580"
     CDS             1990..2970
                     /locus_tag="PKV23_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018185192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HQX83954.1"
                     /translation="MKTFTRRETIGLLGAAAGAFVVRPAYAALPKLAKKDTYKVGFAQ
                     TESNNPWRLAQTKSMQDEATKRGWQLVYTDAAGSAAKQVADVRSMIAQKVDVILLAPR
                     EEKPLIPAVMEAKKAGIPMFLIDRNVDQTLAKGGEDYVAFIGSDFTKEGMQAAEVLTA
                     AVGGKAKIIELQGSTGSSPANDRMAGFAAYIKDHPGMEIIASQSGDFARDKGRQVFET
                     LYQAHPEATALYSHNDEMTMGAIAAMEAAGKVPGKDLIIASIDGTKDATQALIDGKVL
                     VVVECNPKFGPAAFDAVVKYGNGETLEPRLVNTDRVFTKDNAAAYMPEAY"
     gene            3169..4695
                     /locus_tag="PKV23_00585"
     CDS             3169..4695
                     /locus_tag="PKV23_00585"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015316587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HQX83955.1"
                     /translation="MNAIDKSFGSVTALSGAHLEIMPAEVMALVGQNGAGKSTMIKIL
                     NGAFRLDRGSIQFDGKPWAAGSPQQAQRAGVSTIFQEINLIAYRTVAENIYLGRELKR
                     FAGLLDWEKMNQGARALLARFSIELDVTKPLGDFSTAIQQMVAIARAVSFDAKLVIMD
                     EPTSSLDEAEVAVLLNTVRQLKSEGVAVVFVSHKLDELYAVCDRVTVMRDGRTVAVSK
                     MADMSKLQLVAAMLGRDLADVRASGATAFGEGEKAIGEELLIAKSVAVGRRVSDASFD
                     VRKGEIVGLAGLLGSGRTETARAIFAADPKSNGEVRFDGKATNFKSPADAIAAGLGYC
                     SEDRKVQGIIPDMSVADNLTLALLPTLTKMGMIDEAQQRKIVLRFIKDVGIKCSGPGQ
                     KIRELSGGNQQKVLLARWLCMDPKLLILDEPTRGIDVGAKGEIQSLIQQFADKGLGVL
                     MISSELEEIVEGADRVFVLRDGQVVAELEHSEISEQAVMRAMAHGASKDTEFIEVQHG
                     "
     gene            4688..5644
                     /locus_tag="PKV23_00590"
     CDS             4688..5644
                     /locus_tag="PKV23_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018182760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQX83956.1"
                     /translation="MVDYSLLLRRYGVFLAVVLLILFNLAVTPHFATMQTLSVNLTQV
                     CTIVIVGVGMTLVIATGGIDLSVGALMAIAGAMSPMFFTGELFPVPNIYLGIALGIVF
                     PIIIASFFGLFNGWLINRFSIQPIIATLVLFIAGRGIAQVSTNGDLQVFKVPEFQAIA
                     IGRVLGVPFQVWIMVVVVIIAAFVLRRTVFGRQVLAVGGNEEAARLSGVPVKRVKLIV
                     YAVSGFCSGIAGLIVIAMNSAADANLIGLGMELDAIAAVAVGGTLLTGGRATILGTLL
                     GALIIQLVRYTLLANGVPDAAALVVKAGLIILAVWLQRQEHA"
     gene            5644..6690
                     /locus_tag="PKV23_00595"
     CDS             5644..6690
                     /locus_tag="PKV23_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009494157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQX83957.1"
                     /translation="MKNFRITPSLAVILVLVILVSIAALRYDGFLGVYNVQSVLRYNS
                     MFALVALGMCFVIMTGGIDLSVGSVAAMCSVVSALLSSYGLFHGLAGGIAAGILAGLI
                     NGVLITKLKILPFIATLATMLAASGTGLLLAGNQSVSVSYDTYFTNLGQGDLLSFMRP
                     EWVDADEVGFLGSILGFILSFPTPAWIAILAYIIGGVVINHTSFGRHVLAVGGGTEAS
                     RLMGLPVNRTIFFVYVISGALAGLAGVILASQFGAGQPIEGIGWELFAIASVVVGGTL
                     LSGGKGSVAGTLAGALLLGVIFTILNFENGMGWISLSAYWQSVIRGLFLLVVVILQSR
                     LTYKDPADSLKAST"
BASE COUNT         1334 a         1838 c         1978 g         1559 t
ORIGIN      
        1 gtatgattgc cgtgcttggc atccaacagg acttcggcca gcggcacgtt gaacaacctg
       61 acgttgacag agtgaatttt ggtcatggga tccctggcag gcgccaataa atgtttgagt
      121 tcagacatcg agttctaatt aagtctcccc aacaaatcca ttgataatat tgcgttaggc
      181 ggtgtttggt ttgctgtata agctgcccga taacatctga tgaaaagaaa agtgcttcaa
      241 tggcgagacc aggaatgacg gaaaccttcg atcacatcat tgtaggcggg ggatcttccg
      301 gatgcgtggc cgctgcccgc ctcgcaagcg agggcggggc aaaggtgttg ctgcttgaag
      361 ccggctattc gaatcgacac ccgttattgg acatgccgcc aggcatcttc aaaatgatca
      421 acgggtcgaa attcatgcgc taccaccata cgacgcctca ggcgcatttg gacggacgcg
      481 tgcacgatat tccgcagggc aacgttttgg gcggtggatc ttcggtcaac gcccaggttt
      541 atattcgtgg ccggccagcg gattacgacg aatggaacga tctgctgcgc ggcaacaacg
      601 accgcattgg ctggggctgg gacgatgtgc tgccgcattt cacccggatg gaaggcaaca
      661 accggtttat gaatggccgg cacggaacgg atggcccgct gcaggtttcc gatcccggcc
      721 atatcaacga gttttcgcgc tggttcgttc aggccgtgca ggggctggga gagcccttca
      781 attctgattt caacggcccg cttcaacgcg gtgtcggatt ctatcagttc acaaatcgca
      841 acggcaagcg gtcctccgcc gcttacgcct ttctctcggc acaggagaac aatccaaacc
      901 tgacggtgcg actgcaatgc gaggcccagc gcgtggtaat cgaggcgggt cgcgccgttg
      961 gagtgatcta caaggacaag tctggcgcgc tgcggcaggc gaggtgcgag ggcgaagtga
     1021 tcgtggcttc cggtgcgctg gtcacgccaa agcttctgat gctctcaggc atcgggcctg
     1081 ccagccattt ggccgcccat ggcattgcct gcctggttga ttctccaggt gttggacaga
     1141 acctgattga ccatcccgaa gtcccgatca cggcaatcgc caatggcccc ttcggctact
     1201 acaagcaggg cgaggggtgg cgcatgcttc gcaatggctt gcaattcaag ctcttcggca
     1261 caggccccgt tacgtcagcg ggtgtcgagg ctggcgccta tgtgaatccc cgcaatcctg
     1321 aaggtctgcc cagcattcag gccttttgtg tcccgatcgt ctaccttgat cgcgatgccc
     1381 tggacctggt gaaagatact ttcggcatca cggtgaccac cgtagtcgtc aaaccccgct
     1441 cacgaggcga ggtccgtctg gcatcggctg atccctctga aatgccgatg gtttcgccca
     1501 atctgttaaa ggatcccgca gatatgcagg agatggtcga tggccagcgg ttcttcttgc
     1561 gcgccttcga acagaagccg cttgccgaca ggctgcgcca gattgtgatt ccgttacctg
     1621 gcgaccggtc cgatgcggca ttgcaggcgc attgccgccg attcgtcaaa accaactacc
     1681 atcccgtggg cacggcgaaa tttggcgctg acagcgatga tatggccgtg cttgacgctc
     1741 gcctgcgcgt gcggggcgtc gaggggctgc gcgtgtgcga cctgtcggcc atgcccaaca
     1801 tcgttgcggg aaataccaac gcgccagcca tgatgctggg tgaccgttgt gccgatctgg
     1861 tcatgggcaa tctgtaatca cgaggcgtat tgtaatacaa tactagttga ctggaatttg
     1921 cattcgctat ttagtggcca cgagtgcgcg ggaatgaaca agcgcgcccg tttttaaagg
     1981 gagaaatcaa tgaaaacgtt tacacgtcgc gaaactattg gccttttggg tgctgccgct
     2041 ggcgcatttg tggtgcgccc ggcctatgct gcactgccga agctcgcgaa gaaagatacc
     2101 tacaaggttg gctttgccca gaccgaaagc aataacccat ggcgcctggc gcagaccaag
     2161 agcatgcagg acgaggcgac caagcgcggc tggcagttgg tttataccga tgccgccggt
     2221 tccgccgcca agcaggtcgc cgacgttcgc tccatgattg cccagaaggt agacgtcatt
     2281 ctcctagcac cccgcgagga aaagccgctt attcctgcgg tgatggaagc caagaaggct
     2341 ggcattccca tgttcctcat tgaccgcaac gtcgatcaga cgctggccaa gggtggcgaa
     2401 gactatgttg ccttcatcgg atcggatttc accaaggaag gcatgcaggc tgccgaggtt
     2461 ctgaccgcgg ctgtcggcgg caaagccaag atcattgagc ttcagggaag cacagggtct
     2521 tcgccggcaa atgaccgcat ggccggtttc gccgcataca ttaaggacca tcccggcatg
     2581 gaaattattg cgtcgcaatc cggtgacttc gcccgcgata agggtcgcca ggtgtttgaa
     2641 accctgtacc aggcccatcc ggaagcaact gcattgtatt cccacaatga cgaaatgacg
     2701 atgggagcaa tcgctgccat ggaagcggcc ggcaaggttc caggcaaaga tcttatcatc
     2761 gcgtcaattg atggcacgaa ggacgccacg caggcgctca tcgatggcaa ggtgttggtc
     2821 gttgtcgagt gcaacccgaa attcggccct gccgcctttg atgccgtggt gaaatacggc
     2881 aacggcgaaa cacttgagcc gcgccttgtg aataccgatc gcgtattcac caaggacaat
     2941 gccgctgctt atatgccaga ggcttactag aaacgcgcgg gcgtcgatga agcttgcctt
     3001 gttgcaacgg cttatatgaa tgagcgcagg ttgaatttat cgcctcgaca gcaaatgatc
     3061 cgggagttgg cgccgaaaac gccagcgacc ggatcgcact ttttaggcct accttctcct
     3121 caaatgattg gttcataaga tatgagtagc gtggagccac ttctgtccat gaacgcgatc
     3181 gacaagtcct tcggcagtgt tacggcgctt tccggcgccc atcttgagat catgcccgcg
     3241 gaagtcatgg cccttgttgg ccaaaatggc gcaggcaagt cgacaatgat caagatcctc
     3301 aacggtgctt tccgtctgga tcgcggctcc atccagtttg acggaaaacc ctgggcagca
     3361 ggttcgcccc agcaggcgca acgcgcgggc gtcagcacga tatttcagga aatcaacctg
     3421 attgcctatc gcactgttgc agaaaacatt taccttggcc gggagctgaa acgtttcgct
     3481 ggcctgctgg actgggaaaa aatgaaccag ggtgcgcgcg ccctgcttgc ccggttcagc
     3541 attgaacttg atgtgacgaa accgcttggc gacttttcca cggccatcca gcagatggtc
     3601 gccatcgccc gcgccgtctc gttcgatgcc aagctggtaa ttatggatga acccacgtct
     3661 tcgctggatg aagccgaagt ggcggttctt ttgaataccg ttcgccagtt gaagagcgaa
     3721 ggggtagccg tggtttttgt aagccacaag ctcgacgaac tctacgcggt gtgcgaccgc
     3781 gtgaccgtca tgcgtgatgg gcgcaccgta gccgtctcaa aaatggccga catgtcaaag
     3841 ctgcagctag tcgctgccat gctgggacgt gatctcgcgg atgtgcgcgc cagtggcgca
     3901 accgcctttg gcgaaggcga aaaggccatc ggcgaggaat tgctgattgc gaagtccgtg
     3961 gccgtgggcc gcagggtgtc agatgcaagc tttgatgtcc gcaaaggcga gatcgttggg
     4021 cttgctggcc tgcttggatc agggcgcacc gaaacggccc gcgccatctt tgccgctgat
     4081 cccaagtcaa atggggaggt tcgatttgat ggaaaggcca cgaatttcaa aagccctgca
     4141 gacgccattg cagcaggatt gggctactgt tcggaggacc gcaaggttca gggcatcatt
     4201 ccggacatgt cggttgctga caatctgacg cttgccctcc tgcccacctt gacgaaaatg
     4261 ggaatgatcg atgaggcgca gcagcgcaag atagtgcttc gttttatcaa ggatgttggc
     4321 attaagtgtt caggcccggg ccaaaagatc cgggagcttt ccggcggcaa tcaacagaag
     4381 gtccttttgg cgcggtggct ttgcatggat cccaaactct tgattctgga tgagccaacg
     4441 cgcggcatcg atgtcggcgc caagggtgaa atccagagcc ttatccagca attcgccgat
     4501 aagggcctgg gtgtgctgat gatttcgtcc gaacttgagg aaattgtgga aggcgcggac
     4561 cgggtctttg ttctgcgcga tgggcaagtt gtggccgagc ttgaacactc cgaaatatca
     4621 gagcaggccg tcatgcgggc catggcgcat ggcgcctcga aagatacgga attcatcgag
     4681 gtgcagcatg gttgattatt cgctactgct gcgccgctac ggcgtattcc ttgccgtggt
     4741 attgctcatt ctattcaatt tggcggtgac gccccatttc gccaccatgc agacgctttc
     4801 cgtcaatctc acccaggttt gcacgatagt gattgtgggc gttggtatga ccctggtcat
     4861 tgctacagga ggcatagacc tctctgttgg ggcgttgatg gcgattgcgg gtgccatgtc
     4921 gcccatgttt tttaccggcg agttgttccc cgttcccaat atctatttgg ggattgcttt
     4981 gggaattgtg tttcccatca ttatcgccag cttttttggt ctcttcaacg gctggctgat
     5041 caaccggttc tccatccagc ccattatcgc cacgctggtt cttttcatcg caggacgcgg
     5101 cattgcacag gtttcgacca atggtgacct gcaggttttc aaggttcccg aattccaggc
     5161 catcgccatt ggccgcgtcc tcggggtgcc gttccaagtc tggatcatgg tcgttgtggt
     5221 aatcattgcg gcctttgtcc tgcggcgaac cgtcttcggc cggcaggtgc tggccgtcgg
     5281 cggcaatgag gaggccgcgc gcctttccgg cgtgccggta aagcgggtca agctgatcgt
     5341 atatgcggta tccggatttt gcagcggcat tgccggactc attgtcatcg ccatgaattc
     5401 cgcggccgat gccaacctga tcggcctggg catggagctt gatgccattg cggcagtggc
     5461 tgtgggtggc acgctattga cgggtggcag ggcaacaatt ctcggcacgc tccttggcgc
     5521 gctgattatc cagttggtgc gatatacgct gctggccaac ggtgtgccgg atgccgccgc
     5581 cctggtggtc aaggcaggac tgattattct tgcggtttgg ctgcaacgcc aggagcacgc
     5641 ctgatgaaaa atttccggat cacgccgagt cttgccgtca tactcgttct tgtcattctc
     5701 gtttcgattg ccgccctgcg ctatgacggg ttccttggcg tctacaatgt gcagtcggtt
     5761 ctgcgctaca attccatgtt tgccctcgtt gccctcggca tgtgttttgt catcatgacg
     5821 ggaggcatcg acctgtctgt gggaagcgtc gctgcaatgt gcagcgtcgt ttcagcgctg
     5881 ctcagttcct acggactttt ccatggactt gcgggcggta tcgcagccgg cattctggct
     5941 ggcctgataa acggcgtcct gatcacaaaa ctgaagattt tgccgtttat cgccaccttg
     6001 gccaccatgc tggcggcctc cggcaccgga ttgcttctag ccggaaacca gtctgtttca
     6061 gtatcctatg atacctattt caccaacctg gggcagggcg acttgctcag cttcatgcgg
     6121 cccgaatggg tcgatgcgga tgaagtgggc tttctcggct cgattcttgg cttcattctc
     6181 agctttccga cgccggcctg gatcgccatt ctcgcctata tcattggcgg cgtggtcatc
     6241 aaccatacat catttggccg tcatgtcctg gcggtcggcg gtggcaccga agcttcgcgc
     6301 ctgatgggcc tgccggtgaa ccgcaccata ttcttcgttt acgtgatttc cggcgcccta
     6361 gcgggactgg ccggcgtcat cctcgcttcc cagttcgggg caggacagcc gattgaaggc
     6421 ataggctggg aactgtttgc catcgcctcc gttgtcgtcg gcggcacgct attgtcgggt
     6481 ggcaagggct cggttgcggg cacactggcg ggtgcgctgc tactcggcgt gatctttaca
     6541 atcttgaact ttgaaaacgg catgggttgg atttccctgt ccgcctattg gcagtcggtg
     6601 atccgcggtc tgttcctcct ggtagttgtc atcctgcaat cgcggcttac atacaaggac
     6661 ccggcggact cgctcaaagc ttcaacttga tgatttcggc tgggttagg
//
LOCUS       DAORVN010000015         6165 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_8257_length_6165_cov_3.544517, whole genome shotgun sequence.
ACCESSION   DAORVN010000015 DAORVN010000000
VERSION     DAORVN010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 6165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6165
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8257_length_6165_cov_3.544517"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..767)
                     /locus_tag="PKV23_00600"
     CDS             complement(<1..767)
                     /locus_tag="PKV23_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013636964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HQX83958.1"
                     /translation="MTLKIATWNINSVRLRIGLVERVLNQLKPDVLCLQETKCPQGQF
                     PSGPLHDLGYTHIAEFGQKGYHGVAIVSRLPFAEQNSRAFCGKEDCRHVSISLAGSKP
                     ITIHNLYVPSGGDEPDPGINEKFAHKLKFIDELESWFAKGSVPKGQEGVVVGDLNIAP
                     LEHDVWSHKDLLKVVSHTPVETQGLLRVMKAGNWVDLMRQHTPASEKLYTWWSYRAKD
                     WALANRGRRLDHIWSTPGLSPACNDITILRETRGWTQT"
     gene            complement(811..1470)
                     /locus_tag="PKV23_00605"
     CDS             complement(811..1470)
                     /locus_tag="PKV23_00605"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HQX83959.1"
                     /translation="MENDMMRSLKAAALVLVVTIASLVGTSAVSTAAKSIILNPEQTK
                     SINKISDYMNSFKTLQGEFTQISPKGNVSKGVMFISKPGKLRFEYSPPNPFLLVSDGK
                     WVTLKNRAKEKGDQFPLAATPLRLVVAPKVDLLQEANILGFEQADGITSVVLEDRKGT
                     IGGQLVLIFDETQNQLQQWIIVDGKGRRTTVSLANLESGMNIDPKLFVVKIDRKEKDS
                     R"
     gene            complement(1541..4066)
                     /locus_tag="PKV23_00610"
     CDS             complement(1541..4066)
                     /locus_tag="PKV23_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009490638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HQX83960.1"
                     /translation="MAYNRSYDTDDGAIPAALRRFIRHRLFEISGVALLGSLAAVGLA
                     LATWSVDDPSFNHATDQIARNYLGYPGAVIADELMQLLGLGVLPLIGIPMAWVVNLMS
                     HERPERPFTAMLAWLAATFLACGAFSTLPSPSNWSLAAGLGGNAGDIISGGLLSLLAL
                     ALKGAFAQVFTGALCAGTAIWAALRATGLTKSETTGTLGNLGRAAGAVLIRLLRFLQA
                     SYMHWRAYRAQEKSARALRAASANSARDIISRLAPPQKLRIEPSLDRSSKASEPRLAL
                     PELEEELEEDEASDDDEDGDEAVAEAPAVRVTREAKEIKLGKKIKKDSQLNLRFTKSS
                     EFEFPPLTLLAEPKRSTKQSIDLSDEVLEQNARMLEGVLEDFGVRGQIINVKPGPVVT
                     LYELEPAPGIKSSSVISLADDIARSMSAVSARVAVVQGRNVIGIELPNSKRETVYLRE
                     MLASEAFEKTGQNLAFALGKNIGGEPIFADLARMPHLLIAGTTGSGKSVGINTMILSL
                     LYRLSPERCRLIMIDPKMLELSVYEGIPHLLSPVVTDPRKAVVALKWAVREMEDRYRK
                     MSKLGVRNIDGYNARLKQTLAKGEQMSRTVQTGFNPESGEPIFEQEQMDMTSMPYIVV
                     VIDELADLMMVAGKDIEGTVQRLAQMARAAGIHVVMATQRPSVDVITGTIKANFPTRI
                     SFQVTSKIDSRTILGEQGAEQLLGQGDMLYMAGGGRISRLHGPFVADDEVEKIVRFLK
                     AQGAPEYVDAVTEEVEDESYGDPGAMAGTEGGGSGDELYDQAVAVVLRDKKVSTSYVQ
                     RRLQVGYNKAASLIERMEKEGLISSANGTGKREILVPGDSARD"
     gene            complement(4216..5742)
                     /locus_tag="PKV23_00615"
     CDS             complement(4216..5742)
                     /locus_tag="PKV23_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009734729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HQX83961.1"
                     /translation="MTIPPKLRQLLVGLPAGLALTTFLTATPAVAAATVNKGDTTWMM
                     LAAILVILMTIPGLALFYGGLVRSKNMLSVLMQVFICFCMISVLWFIYGYSLAFTNGN
                     AFIGGFSKFMLAGVDYNTLAATFSKETYIPEFAYIVFQLTFAAITPALIVGAFAERMK
                     FSAILLFLAIWFTFAYLPMAHMVWWWAGPDAFTLSADNIDAVKAAVGDAAATDFLAKL
                     AAATDDAAKALVLGDYATAVNASNGWLFNKGAIDFAGGSVVHINAGIAGLVCAIVLGK
                     RLGLGKEVLAPHNLPFAMIGACLLWIGWFGFNAGSNMEANGLTAQVIFNTILAASAAA
                     LAWTAGEWVTRGHPSLLGAFTGAVAGLVAVTPACGWVSPTAALIIGIVAGIVCLWAVV
                     WLKAKLGYDDSLDVFGVHGVGGIVGALLTGYFVNPAIGGVGVTDYLATDTSVKALEYS
                     FSAQMTAQIYSVLTAVILSAVVSYVALMICKYTTGLRVPEQEEREGLDIGTHGERAYS
                     "
     gene            complement(5765..6103)
                     /locus_tag="PKV23_00620"
     CDS             complement(5765..6103)
                     /locus_tag="PKV23_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018701003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HQX83962.1"
                     /translation="MKYIIAIIKPFKLDEIREALGAIGVQGITVTEVKGHGRQKGHTE
                     FYRGAEYAVSFIPKVKIEIAVEKAMADRVVETIQAAGQTGQIGDGKIFVLPLESAVRV
                     RTGETGSAAL"
BASE COUNT         1333 a         1712 c         1851 g         1269 t
ORIGIN      
        1 gtttgagtcc atccccttgt ctcacgcaga atcgtaatgt cgttgcaagc cggagaaagc
       61 cccggtgtcg accagatgtg gtccaacctg cggccccggt tggcgagtgc ccagtccttg
      121 gcccggtagc tccaccaagt ataaagcttc tcgctggcgg gcgtatgctg gcgcatcaga
      181 tccacccagt tgccagcctt catgacgcgc aggaggccct gcgtttccac tggcgtatgg
      241 ctgacgacct tgagaaggtc cttgtgggac cagacatcat gttcaagggg ggcgatattg
      301 aggtctccca cgacgacgcc ctcctggccc ttggggactg agcccttggc aaaccagctc
      361 tccagttcat caatgaattt cagcttgtgg gcaaatttct cattgatccc gggatctggt
      421 tcgtcaccac cggaaggaac atagagatta tggatggtga tgggcttgga tcccgcaaga
      481 cttatggaaa cgtggcggca atcctccttg ccgcaaaagg ccctgctgtt ctgctctgca
      541 aacggcaggc gcgaaactat ggcaacaccg tgataaccct tctggccaaa ttcggcgata
      601 tgggtgtagc ccagatcatg caacggaccg gagggaaact gcccctgcgg gcatttcgtc
      661 tcctgaaggc aaagcacatc aggtttgagc tgattcagaa cccgctcgac caggccgata
      721 cgcaggcgta ccgaatttat attccaggtg gcgatcttga gtgtcatggc cgcagaatga
      781 gcacaggatc aggcgtcaac gcaagtcccg tcagcggctg tctttttcct tgcgatcgat
      841 cttcaccaca aaaagcttgg ggtcgatatt catgcccgat tcaagattgg caagcgaaac
      901 agtggtgcgc cggcccttgc cgtcgacgat aatccattgc tgtaactgat tttgggtttc
      961 gtcgaaaatc aggacgagtt ggccgccaat cgtgcccttg cggtcctcga gcactaccga
     1021 ggtaatgcca tccgcctgct caaaccccaa aatattcgct tcctgcagga gatcaacttt
     1081 tggcgcgacg accaagcgca acggggtggc tgcaagggga aactgatcgc ccttttcctt
     1141 ggcgcggttt ttcagggtca cccattttcc gtccgaaaca agtagaaaag gattaggcgg
     1201 agaatactca aagcgcaatt tgccgggctt gctaatgaac atcacgccct tggagacatt
     1261 tcccttgggg ctgatctggg tgaactcgcc ctggagggtc ttgaaactat tcatatagtc
     1321 ggagatttta ttgattgatt tggtctgctc aggattgaga ataattgatt tggcggccgt
     1381 agagacggcg cttgtgccaa ccaaagaggc gatagtgacc acgagaacca gggcagcagc
     1441 tttaagggat cgcatcatat cattttccaa gcctgagatt ttaagtgctt ctatccatcg
     1501 ttcgtatggc ctgcaaaacc gaccattttg gggcaatcaa tcaatccctg gcgctgtcgc
     1561 cgggaaccag gatttcccgt tttcccgtgc catttgccga tgaaatgagc ccctcttttt
     1621 ccatccgttc tatcaggctt gcggccttgt tgtaacccac ctgcaggcgc cgctgcacat
     1681 agctggtgga gaccttcttg tcacgcagca caacggccac cgcctggtca tagagttcgt
     1741 cgcccgagcc gccgccttct gttccggcca tggcgccggg atcgccatag gactcgtcct
     1801 cgacttcctc ggtgacggca tcaacatatt cgggggctcc ctgcgccttg aggaagcgga
     1861 cgattttctc cacctcgtca tcggccacaa agggcccgtg caggcgcgaa atccgtccac
     1921 cgccggccat gtaaagcatg tcgccctggc ccaggagctg ctcggcgccc tgctctccca
     1981 gaatggtgcg gctgtcaatt ttcgaggtga cctggaagga gatgcgggtg gggaaattgg
     2041 ccttgatggt gccggtgatg acgtcaaccg agggccgctg ggtggccatg accacgtgga
     2101 tgccggcggc gcgggccatc tgggcgaggc gctgaaccgt tccttcaatg tccttgcccg
     2161 ccaccatcat caggtcggcc agttcgtcga tgacgacgac gatgtagggc atggacgtca
     2221 tatccatctg ctcctgctcg aaaatgggct cacccgattc ggggttaaag ccggtctgca
     2281 cggtgcgcga catctgctcg cccttggcca gagtctgctt cagcctggcg ttgtaaccat
     2341 cgatgttgcg aacaccgagc ttcgacatct tgcggtagcg atcctccatt tcgcgaaccg
     2401 cccatttcaa ggcgacaacg gccttgcggg ggtctgtcac gacaggcgaa aggagatgcg
     2461 gaatgccttc atatacggac aactccagca tcttgggatc aatcatgatc agtctgcagc
     2521 gttccggtga cagccggtag agcagggaca gaatcatagt gttgatgccg acggacttgc
     2581 cggagccggt ggtgccggcg atcagcagat gcggcatgcg ggcaaggtcg gcgaagatgg
     2641 gttcgccgcc aatgttcttg ccgagggcaa aagccaggtt ctggccggtt ttttcaaagg
     2701 cttcggaagc cagcatttcg cggaggtaaa ccgtctcgcg cttgctgttg ggaagctcga
     2761 tgccgatgac gttgcggccc tggacaacgg ccacacgggc cgacacggcg ctcatggagc
     2821 gggcgatatc atcggcaagg ctgatcacgc tcgatgattt tatgccaggg gcgggttcta
     2881 attcgtaaag cgttacaacc gggccgggct tcacattgat gatctggcca cgcacgccaa
     2941 aatcctccag cacgccttca agcatgcggg cgttttgctc aagcacttca tcggaaaggt
     3001 cgatggattg cttggtgctg cgcttgggtt cggccagcag ggtgagcggc ggaaattcaa
     3061 actcgctgga cttggtaaag cgcaggttga gctgggaatc cttcttgatt tttttgccca
     3121 gcttgatttc cttggcttcg cgcgtgaccc gcacggcagg ggcttcggca acagcctcat
     3181 ctccgtcctc gtcgtcatcg gaggcttcat cctcctccaa ttcctcttcc agctcgggaa
     3241 gggcaagacg gggttccgaa gctttgctgc tgcggtcaag cgagggttcg atgcgcagtt
     3301 tttggggagg cgctaatctc gaaatgatgt cgcgggcgga atttgccgat gccgcacgga
     3361 gagcccgggc ggatttttcc tgagcccggt aagcgcgcca gtgcatataa ctggcctgca
     3421 ggaagcgcag aagtcgaatt aggacagcgc cggcggcgcg gccgaggttt cccagtgtgc
     3481 cggtcgtttc ggatttggtc agtccagtgg cgcgaagggc tgcccatatg gcggttcccg
     3541 cacacagggc cccggtgaag acctgggcga aagccccttt caaggccaaa gccagaagcg
     3601 acagaaggcc gccgctgata atatctccgg cattgccgcc aaggcctgcg gcaagcgacc
     3661 agtttgatgg cgaaggcaaa gtggaaaagg caccacaggc gagaaatgtg gcggcaagcc
     3721 aagccagcat tgccgtgaac ggccgctcgg gtctttcgtg gctcatcagg ttcacaaccc
     3781 aggccatggg aatgccaatt agaggcagaa ctccgagacc caggagctgc atgagttcgt
     3841 cggcaatgac ggcgcccggg taacccaggt agttcctggc gatctggtcc gttgcatggt
     3901 tgaacgatgg atcatccacc gaccaggtgg ccagggccag gcctacagcc gccagagagc
     3961 ccaggagcgc gaccccggaa atttcaaaga gtcgatggcg tatgaagcga cgcagcgcgg
     4021 ctggaattgc gccgtcatcg gtatcatagg aacgattgta agccatggac tactccgcca
     4081 gcccggagat catcccacag gcccataaca aatggttaat ggcagtctga agggtcatgg
     4141 ttaaagcagg gttaagctcc catgaaaaag cgccccgggc ggggcgcttt tccgttgtgt
     4201 cttgaatggc cgaacctagg agtaggcacg ctcgccgtgg gtgccgatat cgaggccctc
     4261 acgctcttcc tgctcgggaa cacgcaggcc cgtcgtatac ttgcagatca tgagcgccac
     4321 gtagctgacg acggcggaga gaatgaccgc tgtcagcacg ctgtagatct gcgccgtcat
     4381 ctgggccgag aaggaatatt caagtgcctt cacggaggtg tcggtggcga gataatcggt
     4441 cacgccaacg ccgccaattg ccggattgac gaagtagccg gtgagaaggg caccaacgat
     4501 gccgccaacg ccgtggacgc caaagacatc cagggaatcg tcgtaaccaa gtttggcctt
     4561 gagccagacc acggcccaga gacacacgat gccggcaaca atgccaatta tcaaagctgc
     4621 agtggggctt acccagccgc aggcaggggt gaccgccacc aggcccgcaa ccgccccggt
     4681 aaaggcgccg aggagactgg gatggccacg ggtcacccat tcgccggcgg tccatgccag
     4741 agcggccgca gaagctgcca ggatggtgtt gaatatcacc tgggcagtaa ggccgttggc
     4801 ttccatgttg gagccggcgt tgaagccgaa ccagccgatc cagaggaggc aggcgccaat
     4861 catcgcgaac ggcaggttgt ggggagccag aacctctttg ccgaggccaa ggcgcttgcc
     4921 aaggacgatg gcgcagacga ggcctgcgat accggcattg atgtgcacaa cagaaccacc
     4981 ggcgaagtcg atggcgccct tgttgaagag ccagccgttg gaagcattga cggcggtggc
     5041 ataatcgcca agaaccaaag cctttgctgc gtcatcggtc gccgccgcaa gtttggcgag
     5101 gaagtcagtg gcagcagcgt cgcccaccgc ggccttgacg gcatcgatgt tgtcggcact
     5161 cagggtgaag gcgtctgggc ccgcccacca ccagaccata tgggccatgg gcagataggc
     5221 aaaggtgaac caaatcgcca ggaacagcag aatggccgag aacttcatgc gttcggcaaa
     5281 ggcgccgacg atgagggccg gggtgatggc cgcaaaggtc aactggaaaa cgatgtaggc
     5341 gaattccggt atgtaggttt ccttcgagaa ggtcgcggcc agtgtattgt aatcaacgcc
     5401 cgccagcatg aacttggaga atccgccgat gaaggcgttg ccgttggtga aggccagtga
     5461 atatccgtag atgaaccaga ggacggaaat catgcagaag cagatgaaca cctgcatgag
     5521 caccgacagc atgtttttgg agcgcaccaa gccgccatag aaaagggcga ggcccggaat
     5581 ggtcatgagg atcaccagaa tagcggcgag catcatccag gtggtgtcgc ccttgttcac
     5641 cgtggccgcg gcaacagccg gggttgcggt gagaaaagtt gtaagcgcaa gaccggccgg
     5701 caaaccgacg aggagttggc gcaattttgg tggaattgtc atttcactgt ccttttttga
     5761 tctgttagag agccgcagag ccggtttcgc cggtgcggac gcgaacggcg gactcaagtg
     5821 gcaagacgaa aattttgcca tcaccgattt gcccggtctg gcccgccgcc tggattgttt
     5881 ccacaacccg gtcagccatt gccttttcga cggcaatttc gattttgact ttggggatga
     5941 agctcacggc atattcggcg ccgcggtaga actccgtatg gcctttctga cggccatgcc
     6001 ctttgacttc ggtaacagta atgccttgca cgccgatggc gccgagcgct tctcgaatct
     6061 catcgagctt gaacggcttt atgatcgcta tgatatattt catggatagg ttccctccga
     6121 taaccggaag gtgattctca agaaacgtgc caattagaaa cttct
//
LOCUS       DAORVN010000016         6161 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_8268_length_6161_cov_3.113167, whole genome shotgun sequence.
ACCESSION   DAORVN010000016 DAORVN010000000
VERSION     DAORVN010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 6161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6161
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8268_length_6161_cov_3.113167"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..790
                     /locus_tag="PKV23_00625"
     CDS             <1..790
                     /locus_tag="PKV23_00625"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQX83963.1"
                     /translation="KQFSLHGTALQISGSGPPVVLLHGVGLNQSIWAAQVTALESDFQ
                     VITYDLLGHGRSAPVTENAQLSNWVTQLNGVSHDLALEKFSLVGFSFGGLIAQAYATR
                     QSQKIDRLVLMSTVYDRNETERAGVLARLDQAKLEGSQATIPAALSRWFSPEFSKVHL
                     DTIHQYEVLLRSNDAASFLSAYECFARADQEVVEALAGFDRPVLVMTGELDTGSTPAM
                     ARKLAGMIPEAECSIIIGGRHMMPVEMPEAVNSVLLRFLKKGRS"
     gene            787..1332
                     /locus_tag="PKV23_00630"
     CDS             787..1332
                     /locus_tag="PKV23_00630"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HQX83964.1"
                     /translation="MRTLLKAIVWIIAALVVIFIGGGYLLPSEVNMQRETVINAPPEK
                     VFALVGGYKRFNEWSPWAELDPNAVYTFEGPESGVGAKMSWASNDPNVGAGSQTITAH
                     VPNSHVAVDLDFGAMGKSQSTWELKPEGAGTSATWGFKMKLDGMMDRWFGVLMERFIG
                     PDYEKGLAKLKAIAEKEAAGG"
     gene            1325..2797
                     /locus_tag="PKV23_00635"
     CDS             1325..2797
                     /locus_tag="PKV23_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019564952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HQX83965.1"
                     /translation="MAEPFQLYIDGAFGAAEGGATFKSTDPSTGKAWALMPKASAADT
                     DRAVRAASAAFNAGPWPAMTATARGKLLCRLAELVTRDAAIIAELETRDTGKVIRETK
                     AVTGYVAEYYRYFAGLADKIEGATFPIDKPDLEVSTRREPLGVVAAIVPWNSQMFLAA
                     TKLGPALAAGNTVVIKASEDGPAPLLHFARLVHEAGFPAGVVNIITGFGADCGEVLTS
                     HPLVARVAFTGGPETARHVVRNTAENFAVTTLELGGKSPVIVFDDANLDSALNGVIAG
                     IWGATGQSCVAGSRLLVHESIQDEFVARLARRAGGIKIGLPLDMATEMGPLATLKQVQ
                     RAEKLITESLAAGAVLVAGGKSPMGDGYFFEPTILLAPSAELPCAREEFFGPVLSVLK
                     FNTEDEAVALANGTAYAFAAGIFTQNLGRAHRLTKKIRAGVVWVNSYRAVSPIVPFGG
                     NGYTGYGRESGLDAIRDYTRTKSIWINTSDAPMADPFVMR"
     gene            2800..3573
                     /locus_tag="PKV23_00640"
     CDS             2800..3573
                     /locus_tag="PKV23_00640"
                     /inference="COORDINATES: protein motif:HMM:NF004847.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HQX83966.1"
                     /translation="MRTALITGGAQGIGAAVARKFLSEGFSGVLLVDRNAEKLVAVQE
                     QIGKGAEIYTGDLRDGGTPAGAVAKAIKSFGRLDVLVNAAGNTERCGIADTTPEAFDR
                     LFEVNVKAPLFMMQEAAKHMVAAKSGVIINVASMLAHGGPPNLATYSASKSALMTLSR
                     NAGNTFKRDGVRVFCINLGWALTDGEHELQTTFHKLPQSWAEDIGARMPFGRLITSDD
                     VAGLCAFLVSPPAQMMTGAVIDYEQMPLGTFDSHPALSP"
     gene            complement(3563..4456)
                     /locus_tag="PKV23_00645"
     CDS             complement(3563..4456)
                     /locus_tag="PKV23_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015931961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HQX83967.1"
                     /translation="MTRRGLLKWLGGLAIAGASVGTYALAIEPGFLLRTQYYALTPPN
                     WTPGLKLRLVLIADPHLGEPYMSLGRWSRILDLANSLEGDLNLLLGDFAATHRFVTSR
                     IPIADTAHAAKKLTSRLGTYAIIGNHDWWGDATAQSLGHGPTLAERAMEDAGIPVLEN
                     RVARLAKDNLPFWLSGTASIVALKTEVRGRFEGRDDLPGTLAQVTDAAPIIHLAHEPD
                     LFTQMPPRVSLTLSGHTHGGQVRLLGWSPIVPSEFGNRFAYGHIIEDGRHLIVSGGLG
                     CSVLPVRLGVPPEITVVDLTG"
     gene            complement(4453..5379)
                     /gene="gcvA"
                     /locus_tag="PKV23_00650"
     CDS             complement(4453..5379)
                     /gene="gcvA"
                     /locus_tag="PKV23_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010912280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GcvA"
                     /protein_id="HQX83968.1"
                     /translation="MLELFPGLRSLRAFDAAARHLSFTRAASDMGVTPAAISHQIKEL
                     EEQIGVSLFARTSRSMRLTREGEILLAAAAESLDSLNHALQKIRKLKNTRQLKVSASP
                     SIGAKWLVPRLDRFLAEVPGADVRIDVSAAVLDFDRDDIDIAIRFGNGKYPGLRSDLL
                     FQDQVFPVCSPILITRDKPLKTPRDLIQHHQLIHLDWEAQGLPWPNWRMWMLAAGIRD
                     FDDAAGLHFSQTSLAVQAAINGQGVALGDSNLVADDLAAGRLVKPFELSLRAPPQFAY
                     HVISPIETADTHMVKAFREWALREAGVTGHTP"
     gene            complement(5665..>6161)
                     /locus_tag="PKV23_00655"
     CDS             complement(5665..>6161)
                     /locus_tag="PKV23_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011654404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HQX83969.1"
                     /translation="VIAAVNGLAFGGGFEIMISADIIIAAEHATFALPEINSGTLADA
                     ATIKLPRRIPYQVATEMLFTGRRFTAAEAKHWGIINEIVPAADLMARARAVAALLADG
                     PPLVFAAIKETLRETMHLPVQEAFNLVTKRKLPTVDKLYSSEDQMEGAKAFAEKRKPV
                     WKGR"
BASE COUNT         1257 a         1618 c         2025 g         1261 t
ORIGIN      
        1 gaagcagttt tcgctccatg gcaccgcgct tcaaatatcc ggcagcggac cgcccgtggt
       61 cttgctacat ggtgtcggcc tgaaccaatc catttgggcc gcacaggtga cggccctgga
      121 atccgatttt caggtcatta cctatgattt gctggggcat ggccggagcg ccccagtgac
      181 tgaaaatgcc cagctttcta attgggttac gcaactcaac ggtgtttctc acgatctggc
      241 gctggagaag ttctcacttg tcgggttttc ttttggcggg ttgattgcgc aagcctatgc
      301 gacccggcaa tcgcagaaga ttgacaggct ggtgctgatg agcacggttt atgaccgcaa
      361 tgaaacggag cgggcgggtg ttctggcgcg cctcgatcag gccaaattgg aaggctcaca
      421 agccacaatc cctgcggcgc tttcgcgctg gttctccccg gaattttcca aagttcacct
      481 cgataccata catcagtatg aagtgctttt gcggagcaac gacgctgctt cgtttctctc
      541 cgcatatgag tgttttgcaa gggcggacca ggaagtggtg gaagcgcttg caggctttga
      601 tcgtccagtt ctggtcatga cgggtgagtt ggatacgggc tccacgcccg ccatggcgcg
      661 gaaactggcg gggatgattc cggaagctga gtgttctatc attatcgggg ggcggcatat
      721 gatgccggtg gaaatgcctg aagccgtcaa ttccgtgctg ctccggtttt tgaagaaggg
      781 acgttcatga ggacattgct gaaagccatt gtatggatca tcgcggcact tgtcgtaatc
      841 tttatcggcg gtggctatct cctgcccagt gaagtcaaca tgcagcggga aacggtgatc
      901 aatgcgccac cagagaaggt ctttgctctg gtgggcggat acaagcgctt caatgaatgg
      961 tcgccttggg cggagcttga ccccaatgca gtttatacct ttgaaggccc tgaatcgggt
     1021 gtgggtgcaa aaatgagctg ggcttccaat gatcccaatg ttggcgccgg gtcgcagacc
     1081 atcacggcgc atgttcccaa ttcccatgtc gccgtcgatc tcgatttcgg cgcaatgggc
     1141 aagtcgcaat ccacctggga attgaaaccg gagggggcgg gaacgtcggc cacctggggc
     1201 ttcaagatga aacttgatgg catgatggac cgctggtttg gcgtgctgat ggaacggttc
     1261 atcgggcctg actacgaaaa ggggctcgcg aaactgaagg cgattgcgga gaaagaggcg
     1321 gcgggtggct gagccttttc aactttatat cgatggggca ttcggggcgg ccgagggcgg
     1381 ggccacgttc aaaagcaccg atccttctac ggggaaagca tgggccttga tgcccaaggc
     1441 cagcgcggct gacacagata gggcagtgcg cgctgcatct gctgcattca atgctggccc
     1501 atggccggcg atgacagcca cagcgcgggg caagctgctc tgcaggctgg cggagttggt
     1561 aacgcgggac gcggccatca ttgcagagct tgaaacccgc gatacgggca aggtgatccg
     1621 tgaaacgaaa gcagtcacgg gatatgtggc ggaatactat cgttacttcg cggggctggc
     1681 cgacaagatc gagggggcaa ctttcccgat cgacaagcct gacctcgagg tgtcaacccg
     1741 gcgcgaaccc ttgggcgttg tggcggccat cgtgccgtgg aattcgcaga tgtttctggc
     1801 agcaaccaag cttggcccgg cgctggcggc cggcaatacg gtggtgatca aggcatcgga
     1861 agatgggcca gcaccgctcc tgcattttgc caggctggtg catgaagcgg gctttcccgc
     1921 aggcgtcgtc aacatcataa ccgggtttgg cgctgattgc ggggaggttt tgacctcaca
     1981 tccgctggtg gcgcgggttg ctttcactgg cggcccggaa acggcgcgcc atgtggtgcg
     2041 caatacggcg gagaattttg cggtgaccac gctggagctt ggaggaaagt cccccgtcat
     2101 tgtgttcgat gacgcaaatc tggactcggc cttgaacggg gtgattgcgg gcatctgggg
     2161 cgccacaggg caatcctgcg tggcgggatc gcgcctgctc gttcacgagt ccatccagga
     2221 tgaatttgtg gcgcgcctcg caaggcgggc cggaggcatc aagatcggct tgccgctgga
     2281 catggcaacg gagatggggc cgcttgcgac cctgaagcag gtgcagcggg cggagaaatt
     2341 gattacggaa agccttgcgg caggggcggt gctggtagca ggcggcaaat ccccaatggg
     2401 tgacggatat ttttttgaac cgaccatatt gcttgcacca agcgctgaac tgccatgtgc
     2461 gcgcgaggag ttctttggcc cggtgctctc cgttctcaag ttcaatacgg aagatgaggc
     2521 ggttgccctt gccaacggaa cggcttacgc gtttgccgcg ggcattttca cgcagaacct
     2581 gggccgggcg caccggctga cgaagaaaat cagggcaggg gtcgtgtggg tgaatagcta
     2641 tcgagcggtt tcgcccattg tgccttttgg cggtaatggc tacacgggct atgggcgcga
     2701 aagcggcctg gatgccatcc gggactacac gcgcacgaaa tcaatctgga tcaatacttc
     2761 ggatgctccg atggctgatc cctttgtcat gaggtagcga tgagaacggc tttgatcacc
     2821 ggcggcgcac agggcattgg tgcggcggtg gcgcgcaaat tcctgagtga gggattttcc
     2881 ggcgtgctgc tagtggaccg caatgcggaa aagctggttg cggtgcaaga gcaaatcggc
     2941 aagggcgctg aaatctatac gggagatttg cgggatgggg gtacaccggc gggtgccgta
     3001 gccaaggcga tcaagagttt cgggcggctg gatgtgttgg taaatgccgc cgggaacacg
     3061 gagcgctgcg gaatcgcgga cacgacgccg gaagcctttg accgcctgtt tgaggtgaat
     3121 gtgaaagcgc cgctgttcat gatgcaggaa gcggcgaagc acatggtggc ggcgaaatcc
     3181 ggcgtgatca tcaacgtggc cagcatgctg gcgcatggcg gcccacccaa cttggctacc
     3241 tattctgcct ccaaatcggc attgatgacg ctgtcgcgga atgcgggaaa tactttcaaa
     3301 cgagatgggg tccgggtgtt ctgcataaat ctcggatggg cgctgaccga tggcgaacac
     3361 gaattgcaaa cgacctttca taagctgccg cagagctggg ccgaggacat cggcgcgcgc
     3421 atgcctttcg gccggttgat cacctcggat gatgtggcgg gactctgcgc ctttctggtt
     3481 tcaccgccgg cgcaaatgat gacaggtgcg gtaattgact acgaacagat gcccctgggc
     3541 acgttcgaca gccatccggc acttagcccg taagatcaac cacggtgatt tcgggcggca
     3601 cgcccaggcg aaccggcagg accgagcagc ccagcccgcc ggatacaatg agatggcggc
     3661 catcttcgat gatgtggcca taggcaaagc ggttgccgaa ctcggagggc acgatgggcg
     3721 accagccaag aaggcgcacc tggccgccgt gggtgtggcc actcagggtg agggagaccc
     3781 ggggtggcat ctgggtaaac aggtcgggct catgggccaa atggatgatc ggggccgcat
     3841 cggtgacttg cgccagcgtg ccggggagat cgtcgcgccc ttcaaagcgg ccacggactt
     3901 ctgtctttaa ggcaacgatg cttgcggtgc cggaaagcca gaaagggaga ttgtccttgg
     3961 caaggcgtgc cacccggttc tcgagaacgg gaataccggc atcctccatg gcgcgctcgg
     4021 cgagggtcgg tccatgaccc aggctttggg ccgtggcgtc gccccaccag tcatggttgc
     4081 caatgatggc ataggttccc agccgtgaag tgagtttctt tgcggcgtgc gccgtgtcgg
     4141 caatagggat gcggctggtt acgaaacgat gggttgccgc gaagtctccg agcagaagat
     4201 tgaggtcgcc ttccagagag tttgccaggt caagaatgcg actccaccgg ccaagactca
     4261 tgtagggttc gcccagatgg ggatcggcaa tcagcacaag acgaagcttg aggccgggcg
     4321 tccaattcgg cggggtgagg gcatagtatt gggtgcgcag caggaagccg ggttcgatgg
     4381 ccagcgcata agtgccgaca gaggcgcctg cgattgcgag gccgccaagc catttcaaca
     4441 ggccgcggcg cgtcacgggg tatggcctgt aacgcctgct tccctgaggg cccattcgcg
     4501 gaaggctttc accatgtgcg tgtccgccgt ctcaatgggg gagatgacat ggtaggcaaa
     4561 ttgcggtggc gcgcgcagcg agagttcgaa gggcttgacc aaacgcccag ccgcaagatc
     4621 atccgccaca agatttgaat cgcccagcgc cacgccctgg ccattgatgg cggcctggac
     4681 ggccagggag gtttggctga aatgcagacc cgcggcatca tcgaaatcgc ggatgccggc
     4741 ggccagcatc cacatccgcc agtttggcca gggcaggccc tgggcttccc aatccagatg
     4801 aatgagctgg tgatgctgaa tgagatcgcg cggcgtcttc aaaggcttgt ccctggtgat
     4861 caggattggg ctgcagaccg ggaaaacctg atcctggaac agcaggtcgg agcgcaggcc
     4921 cgggtatttt ccattaccga agcggatggc aatgtcgatg tcgtcccggt caaaatcgag
     4981 aactgcggca gatacgtcaa tgcgcacatc ggcgccgggc acctcggcca ggaagcggtc
     5041 gaggcgcggt accagccatt ttgcgccgat cgagggcgag gccgagactt tcaactgtct
     5101 ggtgtttttg agtttgcgga ttttctgcag ggcgtggttg agggaatcga gcgattcagc
     5161 ggccgcagcg agcaggattt cgccttcgcg ggtgagcctc atgctacggc tggtgcgggc
     5221 aaacagtgaa acgccaatct gttcctcgag ctccttgatc tgatggctga tggcggcggg
     5281 ggtaaccccc atgtcggagg ctgcacgggt aaagctcaaa tgccgggcgg cggcatcgaa
     5341 ggcgcggagg gaacggaggc caggaaatag ctcgagcatg gcatatcttc aaatttcatt
     5401 tgcagatgaa ttgaaaacta gtcgtttgtg caaacaaata caatcaccta tgttgtgatc
     5461 ataaacaaat actaatgaaa gattaattcc atgtctcgct ttaccagcca aaccgcccca
     5521 acaagctacg gttattcgcc agttggagct actgacagat tgtggctctt cctgaagggc
     5581 atggtccgct tcgagccagt ggcatctgcc atgtatgctg aaaacgacag ggttcggatg
     5641 agcggacgca attggctgat gtaatcagcg gcctttccag acgggcttgc gcttctcggc
     5701 aaaggccttg gcgccttcca tttggtcttc gcttgagtag agcttgtcca ccgttggcag
     5761 cttccgtttg gtcaccaagt tgaacgcttc ctgcacgggc aggtgcatgg tttcgcgcag
     5821 ggtttcctta atggcggcaa agaccagcgg cgggccgtcg gccagcaacg ccgccacggc
     5881 gcgggcccgg gccatgagat ctgcggcggg cacgatttca ttgatgattc cccagtgctt
     5941 ggcttcggct gcggtaaagc gcctgccggt gaagagcatt tcggttgcca cctgataggg
     6001 gatgcggcgt ggcaacttta tggtggcggc gtccgccagg gtgccggaat tgatttcggg
     6061 cagggcaaag gtcgcatgct cggcggcgat gatgatgtct gccgaaatca tgatttcaaa
     6121 accgccgcca aaggccaggc cgttcacggc ggcgatcacc g
//
LOCUS       DAORVN010000017         6037 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_8581_length_6037_cov_3.481946, whole genome shotgun sequence.
ACCESSION   DAORVN010000017 DAORVN010000000
VERSION     DAORVN010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 6037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6037
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8581_length_6037_cov_3.481946"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            60..3353
                     /locus_tag="PKV23_00660"
     CDS             60..3353
                     /locus_tag="PKV23_00660"
                     /inference="COORDINATES: protein motif:HMM:NF013625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HQX83970.1"
                     /translation="MKPGIPWSVKGIEPETREAAKEAARRSGMTLGEWLNSKILDSAD
                     DQESAPPPRHQRAAPARAETSIRLEDIAEQLSRIARREQQTAPAGYVSETRLEDEENL
                     NKILNRIESNERQTVEAFTAVNERLAVLSRQVLQTAKMPTMAKAEEPAALKSLETAVR
                     NIVEHMEISERRTRDSLKAMQDRMAEMAQRATSSTSEQILQSAPAFTSLESRLSELAS
                     RVERTESKSDAGPSDVLRTELSRLAERIEAVRETSEQLASKAQTAAVQATQAELREIE
                     KRILSLLKEAQTAFSGQGGSTSAEIQKLRSEIAALNQRIDSARADTASDRDVHALRVA
                     LEQLSTRVAQGPDMRPLADMDRRLVDISQRLEQSQNLAGSNPQVSDLERRMAELDYRL
                     DEAMRAQGDGQAQIALEQKISEVADRVGQTEHQLGHLETIERAINQLFDSVEQSRDWA
                     KDVAEDAANRMADRILNAPGATPLSLGSSPELKALEDGLQAVRESAIGADQRNQETLR
                     AVHDTLEQVVSKLAELETAAVGQQVAAAAAHGTHAAPAEPAAAGNWQDETYTEEPAPD
                     AEAPQPEAASLPSSSEAERHAPEASHANPLMDWLSKSTMPGGEATSPDDSDPANDDFI
                     TAARRAAQASAQKSILASLAPSAKSGRKRMNLPFMKAASKPAREIPTGEKLPPEIKPD
                     ITKTSNKRRTLVLAGLVLLAAVSAFTFNMLSKPQKSSSSQPSAPIEDSVQPGLPPKQS
                     NSTSPAASSQALAEKTVDKVPMAGSEGPSSIDEILTGSMPNQKTDATLASIVAEPGTA
                     TGEREMPPAELGSQALRNAAERGDASAQFIVATRYLDGEGVTQDVTRAAHWYQKAALA
                     GLAPAQYRLATLFERGRGVPKDTATALVWYERAAAQGNVKAMHNAAVIAAGTEAGKPN
                     YDMAYKWFLAASNNGLKDSQFNLAVMYERALGTKANVDEALFWYLVAASQKDEDARKR
                     AEVLSQNLSPLVVETVRARARAWAPKQAPENANVVTIQDPAWQAPQSVSQSPPAETAV
                     EKQQAAADPIGEAQRLLNKLGFNIGEPDGRMGVRTTNAIRLFQLQYGLKVTGEVTADL
                     ISQLQKRAG"
     gene            complement(3362..4045)
                     /gene="pdeM"
                     /locus_tag="PKV23_00665"
     CDS             complement(3362..4045)
                     /gene="pdeM"
                     /locus_tag="PKV23_00665"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR04123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response
                     endonuclease PdeM"
                     /protein_id="HQX83971.1"
                     /translation="MSKEHRISLNGLDFIPDLSGALFAPAFKALLIADLHLEKASNMA
                     RRGVHLPPYDTRASLAQLEAVIAQIRPEQLIFLGDSFHDDGARERIDVEDLARLRRIS
                     ESAATVWITGNHDPHPPTDIGGRIADHVVLGGVTLRHEPAQLGEEDFEISGHLHPGSA
                     VSQRGKRIHCKCFIADGRRLIMPAFGSFTGALSISAPPFRGLYDERNFHVWMLSQRAI
                     YKFPGKRVS"
     gene            complement(4038..>6037)
                     /locus_tag="PKV23_00670"
     CDS             complement(4038..>6037)
                     /locus_tag="PKV23_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007200069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ligase-associated DNA damage response DEXH box
                     helicase"
                     /protein_id="HQX83972.1"
                     /translation="SKRGVLLSLALARAQSIAPEARRIGLSATVADPEALQHWLAPTG
                     NGPVPLVMGSAGAAPLIRILKSAERVPWSGHSSRYAIEEVYEEITRAKMTLIFVNTRS
                     QAEMTFQWLWEANEANLPIALHHGSLDVGQRRKVEAAMAAGKLKAVVCTSTLDLGIDW
                     GDVDLVIHVGAPKGSSRLLQRIGRSNHRLDSPSEALLVPSNRFEVLECEAALQAAKEN
                     AQDTEYPAVLKLDVLAQHILGRACAGPFDADEFFTEVTRAWTYRHLTRADFDAAMDYV
                     ATGGYALRAYEKYAKLKRREDGLWRLTHPLIAQQYRLNMGTIVEAAMLKVRIASVKRR
                     GGKRILTGGRSLGELEEWFLSQLSIGDTFRFAGEVLRFEGLDEFGALATRTFDSDPMV
                     PSYEGGKFPLSTYLAERVREILSSPAKWKTLPLQVQDWLAMQRQKSVIPRAHQMLVET
                     FPRAEKHYLVCYPFAGRLAQQTLGMLLTRRLERWGAQPLGFSASEYALVIWTVRDVSL
                     MISSGRLSLERLFDEDMLGDDLEAWLAESNLMKRTFRNAAIIAGLIERRWPGKEKTGR
                     QVTVNSDLIYDVLRSHQPDHILLRAAWDDAADGLIDIHRLGNLLKRIKGQLLHRALEM
                     VSPLAVPALLEISRESIYGEAHDALLSEASGALIDEAMGLV"
BASE COUNT         1235 a         2005 c         1788 g         1009 t
ORIGIN      
        1 gagattcgtc tttcgggaaa cacggggaat ccaaggtgac gtcaaaagtg gaagagcgaa
       61 tgaaaccggg aattccgtgg agtgtaaagg gcatcgagcc ggaaacccgc gaggccgcga
      121 aggaggcggc gcgtcgctcc ggaatgactc ttggcgaatg gctcaactcc aaaatcctcg
      181 attccgccga tgaccaggaa tctgccccgc cgccacgcca tcagcgtgcc gcgccggccc
      241 gcgctgaaac ctcgatccgc cttgaagaca ttgccgaaca gctctcccgc atagcgcgcc
      301 gtgagcagca aacggccccc gccggatatg tctctgagac ccggctggag gatgaggaga
      361 acctcaacaa gattctcaat cgcatcgaaa gcaacgagcg ccagaccgtt gaggccttca
      421 ccgccgtcaa tgagcgcctc gctgtcttga gccggcaggt gctgcagacc gccaagatgc
      481 cgacaatggc caaggcggag gagccggccg ccctcaaatc cctggaaacc gctgtccgca
      541 atattgtcga gcatatggaa atcagcgagc gccgcacccg cgacagcctg aaggccatgc
      601 aggaccgcat ggcggaaatg gcccagcgcg cgacttcgtc caccagcgag cagatcctgc
      661 aatcggcccc ggccttcacc agccttgaat cacgcttgag cgagctggcc agccgggtgg
      721 agcgcaccga gagcaagtct gatgcgggcc cttccgatgt cctgcgcacc gagctttccc
      781 ggctcgccga gaggatcgaa gcggtgcgtg aaacctccga gcagctggcc tcaaaggccc
      841 agaccgccgc ggttcaggcc acgcaagccg aacttcgcga aattgaaaag cgcatcttga
      901 gcctcttgaa ggaggcgcaa accgcgttct ccggccaggg cggcagcacc agcgccgaaa
      961 tccagaagtt gcgctcggaa atcgccgcac tcaatcaacg gatcgacagc gcccgcgccg
     1021 atactgcctc ggatcgcgat gtccatgcgc tccgcgttgc ccttgagcaa ctctcaaccc
     1081 gcgtcgccca gggcccggat atgcggcctc tggccgacat ggaccggcgc ctcgttgata
     1141 tcagccagcg ccttgagcag agccagaatt tggcgggcag taatccccag gtgagcgatc
     1201 ttgagcgccg catggccgag cttgactacc gcctcgacga ggcaatgcgc gcgcaaggcg
     1261 atggccaggc ccagatagcc cttgaacaga aaatctccga agtcgccgac cgcgttggcc
     1321 agaccgaaca ccagctcggc catctcgaaa ccatcgagcg tgccatcaac cagttgttcg
     1381 acagcgtcga gcagagccgg gattgggcca aggatgtggc cgaggatgcc gccaaccgga
     1441 tggcggaccg catcctgaat gccccgggcg caaccccgct ctcgctggga agctcaccgg
     1501 agttgaaggc cctcgaggat ggcctgcagg cggtgcgtga aagcgccatc ggcgccgacc
     1561 agcgcaatca ggaaaccctg cgggccgtcc acgatacgct tgagcaagtg gtcagcaaat
     1621 tggccgaact ggaaaccgcg gccgttggcc agcaggtagc cgcagccgcg gcccatggca
     1681 cacatgcagc gcctgccgag ccggccgctg ccggaaactg gcaggatgag acctacactg
     1741 aggaaccggc gccggatgcc gaagcgcccc agcccgaagc cgcgtcattg ccgtcgtcct
     1801 ccgaagctga acgccatgcg ccggaagcgt cacatgccaa tcccctgatg gattggctct
     1861 ccaaatcgac aatgccgggc ggggaggcca cttcgcctga cgattcggat cccgccaacg
     1921 acgacttcat caccgccgcc cgccgcgccg cccaggcctc ggcgcagaag agcattctcg
     1981 ccagcctcgc gccaagcgcc aagtcgggcc gcaagagaat gaacctgccc ttcatgaagg
     2041 ccgcttcaaa gccggcccgc gagattccta ccggcgaaaa actgccgccg gaaatcaagc
     2101 cggacatcac caagaccagc aacaagcgcc gcactctggt gctcgcaggt ttggtgctgc
     2161 tggcggccgt gtctgccttc accttcaaca tgctcagcaa gccgcagaag tccagctcga
     2221 gccagccttc cgccccgatt gaagactcgg ttcaacccgg ccttccgccc aagcagagca
     2281 actcaacatc tccagccgcg agcagccagg ctcttgcgga gaagaccgtt gacaaggtcc
     2341 cgatggctgg cagcgaaggg cccagctcca tcgacgaaat cctcaccggt tccatgccga
     2401 accaaaagac cgatgccact ctcgcctcaa ttgtcgccga acctggcacg gcaacaggtg
     2461 aacgtgaaat gcctccggcg gaattaggct cgcaggccct gcgcaacgcc gccgaaaggg
     2521 gcgatgcgtc ggcgcaattc attgtcgcca cccgctatct cgatggcgaa ggcgtcaccc
     2581 aggacgtgac ccgcgccgcc cactggtatc agaaggcagc ccttgccggc ctggctccgg
     2641 cccaataccg ccttgcgacg ctgtttgagc gcggccgtgg tgtgcccaag gatacggcga
     2701 ccgcccttgt ctggtatgag cgcgccgccg cccagggcaa cgtcaaggcc atgcacaacg
     2761 ctgccgtgat tgccgccgga accgaggccg gaaagcccaa ttacgatatg gcctacaaat
     2821 ggtttctggc cgccagcaac aatggtctga aggacagcca gttcaacctc gctgtgatgt
     2881 acgagcgcgc cctgggtacg aaagccaatg tcgacgaagc cctgttctgg tatctggtcg
     2941 ccgcgagcca gaaagacgag gatgcaagga aacgtgccga agtgctttcg caaaacctct
     3001 cgcccctcgt ggttgaaacc gttcgcgcca gggcccgggc ctgggccccg aagcaggccc
     3061 ctgaaaacgc caatgtggtg accatccagg atcctgcctg gcaggctccc cagtctgttt
     3121 cgcaaagccc gcccgctgaa accgccgttg aaaagcagca agcagcagcc gatcccatcg
     3181 gcgaagccca gcggcttttg aacaagctcg gcttcaacat tggcgaaccc gatggccgga
     3241 tgggagtccg caccaccaac gccatccgcc tgttccagct ccagtacggc ttgaaggtga
     3301 ccggcgaggt taccgctgat ctcattagcc agttgcagaa acgggcaggc tagggcctaa
     3361 gttagctgac gcgcttgccg ggaaatttgt agatcgcccg ctggctcagc atccagacat
     3421 ggaaatttct ttcatcgtag agccctcgga acggcggcgc tgagatgctg agggcgccgg
     3481 tgaaacttcc gaacgccggc atgatcaggc ggcggccgtc ggcgatgaag catttgcagt
     3541 ggatgcgttt gccccgctgg ctcaccgcac ttcccggatg cagatggccg gaaatttcaa
     3601 aatcttcttc ccccaattgt gccggttcat gccgcagcgt caccccaccc agcaccacgt
     3661 gatcggcaat gcgtccgcca atgtcggttg gcggatgcgg atcatggttg ccggtaatcc
     3721 agactgttgc ggcactttcc gaaatgcgcc gcagccgcgc cagatcctcc acgtcaatcc
     3781 gctcgcgcgc cccgtcgtcg tgaaaactgt cgccgaggaa aatcagttgc tctggcctga
     3841 tctgggcaat gactgcttcc agttgcgcca gcgaggcccg cgtatcatag ggtggcagat
     3901 gcaccccgcg ccgcgccatg ttggaggcct tctccagatg caggtcggcg atgagcagcg
     3961 ccttgaacgc gggagcgaac agcgcgcccg aaagatcggg gatgaaatcg agcccgttga
     4021 ggcttatgcg gtgctcctta gacaagcccc atggcctcgt cgatgagggc gccggaagct
     4081 tcggagagaa gggcatcatg ggcctcgccg taaattgatt cgcggctgat ctcaagcagc
     4141 gccggaactg cgaggggtga taccatttca agcgcccgat gcaggagctg gcccttgatg
     4201 cgcttcagca ggttccccag ccggtggatg tcgatgagcc cgtcggccgc atcgtcccac
     4261 gccgcccgga gcaggatatg gtccggctgg tgcgagcgca gcacgtcata gatcagatcg
     4321 gaattgaccg tcacctgccg cccggttttt tcctttcccg gccagcgccg ctcaatcagt
     4381 ccggcaatga tcgccgcatt gcggaaggtg cgcttcatca gatttgattc cgccagccac
     4441 gcctcgaggt catcgcccag catgtcttcg tcgaaaagcc tttccagcga cagcctgccg
     4501 gatgaaatca tcagcgagac atcgcgcacc gtccagatga ccagcgcata ttcggaagcc
     4561 gaaaacccca gcggctgcgc cccccagcgc tccagccgcc ttgtcagcag catgcctagg
     4621 gtttgctggg ccagccgtcc ggcaaagggg tagcacacca ggtaatgctt ctccgcccgc
     4681 ggaaaggttt caaccagcat ctggtgggcc cgcgggatga ccgacttctg gcgctgcatg
     4741 gcaagccagt cctgaacttg cagcggcagg gttttccact tggccgggct gctcaggatt
     4801 tcgcggaccc gctccgccag ataggtcgag agcgggaatt tcccgccctc gtaggatgga
     4861 accatggggt cgctgtcaaa agtgcgcgtc gccagggcgc cgaattcatc gaggccttca
     4921 aaacgcaaaa cctcgccggc aaaacggaac gtgtcgccga tcgaaagctg gctgaggaac
     4981 cattcttcca gttcccccag gctccgcccg ccggtgagga tccgcttgcc gccccggcgc
     5041 ttcacgctgg cgattcgcac cttcagcatc gccgcctcga caatggttcc catgttcagc
     5101 cggtattgct gggcaatcag cggatgcgtc agacgccaca gcccgtcttc ccggcgtttc
     5161 agcttggcat atttctcata ggcgcggagc gcgtatccgc cggtcgccac atagtccatt
     5221 gctgcatcga aatctgcccg cgtcaggtgg cgataggtcc aggcccgcgt cacctcggta
     5281 aaaaattcgt ccgcatcgaa tggccccgcg caggcccgcc ccagaatatg ctgggccagc
     5341 acatccagct tgagaaccgc cgggtattcc gtgtcctggg cattctcctt ggccgcctgc
     5401 agcgccgcct cgcattccag cacctcaaac cggttggacg gaaccagcag cgcctcgctc
     5461 gggctgtcaa gccggtggtt ggaacggccg atgcgctgca gcaggcggct ggagcccttg
     5521 ggtgccccca catgaatgac aaggtccaca tcgccccagt caatgccgag atcaagcgtc
     5581 gaggtgcaga ccacggcctt cagttttccc gccgccatgg ccgcttccac cttgcgccgc
     5641 tggcccacat cgagagaacc atggtggagg gcgatgggaa ggttcgcttc attggcctcc
     5701 cacagccatt gaaaagtcat ttccgcctgg ctgcgcgtgt tgacgaaaat cagcgtcatc
     5761 ttggcccggg tgatttcctc atagacttct tcgatcgcat agcgcgagga atgccccgac
     5821 cacggcaccc gctccgccga tttcagaatg cggatgagcg gcgccgcacc cgcacttccc
     5881 atgaccaacg gaaccgggcc gtttcctgtt ggcgccagcc agtgttgcaa agcttccgga
     5941 tcagccacag ttgcggaaag gccgatgcgc cgcgcctccg gtgcaatgct ttgcgcccgc
     6001 gcaagcgcca gcgacagcag cacgccccgc ttggagg
//
LOCUS       DAORVN010000018         6020 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_8614_length_6020_cov_3.711819, whole genome shotgun sequence.
ACCESSION   DAORVN010000018 DAORVN010000000
VERSION     DAORVN010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 6020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6020
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8614_length_6020_cov_3.711819"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..732
                     /locus_tag="PKV23_00675"
     CDS             <1..732
                     /locus_tag="PKV23_00675"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HQX83973.1"
                     /translation="TEKGTIACEQVVLAGGAWSRRFLHNLDIAFPQLTVVNSVMRTET
                     LDAGVEKSCSAGKFAFRKREDGGYTIAHRHFSVADIMPDSFRQFFQFLPALKMGWSGV
                     RLRLGRRFLDEARLKRRWKLDERSPFEEVRMLDPEPVNWILNEAAASLRDYYPRFQAM
                     RIAERWAGVIDATPDVVPCISPMPKIEGLYLASGFSGHGFGLGPGAGKLMAQMVMGES
                     PCVDPAPFRHGRFLDGSRPQPTTGL"
     gene            complement(733..1131)
                     /locus_tag="PKV23_00680"
     CDS             complement(733..1131)
                     /locus_tag="PKV23_00680"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQX83974.1"
                     /translation="MTNTDKHVDVLILDDDIDLLTTTAELLADVGYKVKGFADPESAL
                     EFAIGQTFSVGLFDFRLGSLKNGLDVVETMQALGCKSVYVMVSADVESSTRLRAINLK
                     LFDFIRKPVAPEVLLETISRALAHSQKMAA"
     gene            complement(1251..2639)
                     /gene="lpdA"
                     /locus_tag="PKV23_00685"
     CDS             complement(1251..2639)
                     /gene="lpdA"
                     /locus_tag="PKV23_00685"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013500291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HQX83975.1"
                     /translation="MAEQFDLTVIGTGPGGYVCAIRAAQLGMKVAVIEKRAAHGGTCL
                     NIGCIPSKALLHASELFAEAKHSFAKMGIAVSPSFDLPKMMVFKQEAIDGNTKGIDFL
                     MKKNKITVIRGTGKILGTGKIEVAGQIVETKNIVIATGSDVAKLKGIEIDEKQIVSST
                     GALELAKVPTHLAIIGAGVIGLELGSVYARLGAKITVIEYLDRILPGMDLEVAKNFQR
                     LLQKQGFTFKLSSKVTSATKAKSGVTLQIEPNTGGAAESLEADTVLVAVGRTPFTEGL
                     GCEKAGIKRDDRGRIEVGGHYETSLEGVYAIGDVIRGPMLAHKAEDEGVALAELLAGE
                     AGHVNYDVIPGVVYTTPEVAAVGKTEEELKAAGVDYKAGKFLFLANGRAKANQTTDGF
                     VKILADARTDRVLGAHIIGPVAGELIHEIAVLMEFGGSAEDLARTCHAHPTMSEAVKE
                     AAMAVAGRSIHS"
     gene            complement(2658..3416)
                     /locus_tag="PKV23_00690"
     CDS             complement(2658..3416)
                     /locus_tag="PKV23_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010912328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQX83976.1"
                     /translation="MAQKPDVAIVTGGSRGIGAATCLLLAKAGYLVVVNYVSNAAAAK
                     SVVAKIEKAGGKATAIHGDVASENDIVALFKSADQLGQLKVLVNNAGVVDTKARVDEM
                     SGERLARMFAINITGSFLCAREAVKRMSTRHGGTGGAIVNLSSAAATLGAPGMFVDYA
                     ASKGAIDVFTLGLAREVASEGIRVNGVRPGIIDTDIHASGGEPDRVAQMRGQLPMQRE
                     GTSLEVAEAIIWLASEKASYVTGTCLGVSGGRSI"
     gene            complement(3428..4621)
                     /gene="odhB"
                     /locus_tag="PKV23_00695"
     CDS             complement(3428..4621)
                     /gene="odhB"
                     /locus_tag="PKV23_00695"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018012726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HQX83977.1"
                     /translation="MAKEIRVPALGESVTEATIAKWFKNVGDAVKADEPMVELETDKV
                     TVEVPAPASGKLTSIAAAAGTTVNVGALLGAIEEGAAGAAAKPAAAAPVAAPAPAKIA
                     EPVKAEAPLSPAVRKIVTENKLDPATIPATGRDGRLTKGDVIEQLARPAAAPASLPAA
                     APAPSGGEERVKMSRLRLTIARRLKEAQNTAAMLTTFNEVDMSAVMTLRNSFKDVFEK
                     KHGVKLGFMSFFAKAVIQALKDVPAVNAEIDGDDLVYKNYYHLGIAVGTEKGLVVPVV
                     RDADRMSFATIEKTVADFGKKARDGQLQIADMQGGTFTISNGGVYGSLMSTPILNAPQ
                     SGILGMHKIQDRPVALGGQVVIRPMMYLALSYDHRIVDGKEAVTFLVRVKESLEDPQR
                     ALLDL"
     gene            complement(4644..5960)
                     /gene="sucA"
                     /locus_tag="PKV23_00700"
                     /pseudo
     CDS             complement(4644..5960)
                     /gene="sucA"
                     /locus_tag="PKV23_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009211416.1"
                     /note="SucA; E1 component of the oxoglutarate
                     dehydrogenase complex which catalyzes the formation of
                     succinyl-CoA from 2-oxoglutarate; SucA catalyzes the
                     reaction of 2-oxoglutarate with dihydrolipoamide
                     succinyltransferase-lipoate to form dihydrolipoamide
                     succinyltransferase-succinyldihydrolipoate and carbon
                     dioxide; incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
BASE COUNT         1162 a         1713 c         1806 g         1339 t
ORIGIN      
        1 acggaaaagg ggacaatcgc gtgcgagcaa gtggtgctgg cgggcggggc gtggtcgcgg
       61 cgcttcctgc ataatcttga tattgcgttc ccacagctta cggtagtgaa ttccgtgatg
      121 cggacagaga cccttgacgc aggcgtggag aagtcctgct cggccggaaa attcgcgttt
      181 cgcaaacggg aagatggcgg ttacacgatt gcccaccggc atttctcggt ggccgatatc
      241 atgccggaca gtttccgcca gtttttccag ttcctgccgg cgttgaaaat gggttggagc
      301 ggggtgcgcc tcagacttgg ccgacggttt cttgatgagg cccggctcaa gcggcgctgg
      361 aagcttgacg agcggtcacc ctttgaggag gtacgcatgc ttgatccgga gccggtcaat
      421 tggattctca atgaggcggc ggcaagctta agagactatt atccacgctt tcaagccatg
      481 cggattgccg agcgctgggc cggcgtcatt gatgcaacgc ccgatgtggt gccgtgcatt
      541 tccccgatgc ctaagatcga gggactttat ctcgcgtccg ggttttcagg ccatggcttt
      601 ggtttggggc cgggggcggg caagctcatg gcgcaaatgg tcatgggtga aagcccttgc
      661 gttgacccgg caccgtttcg ccatgggaga tttcttgatg gcagcagacc gcagccgaca
      721 actggcctgt aatcaggcag ccattttttg cgaatgggcc agggcccggc taatggtttc
      781 aagcaggact tcaggggcca ctggcttgcg gatgaaatca aagagcttga ggttgatggc
      841 gcggaggcgg gttgaacttt cgacatccgc agaaaccatg acgtaaaccg acttgcagcc
      901 cagggcctgc atggtttcaa ccacgtcgag gccgttcttg aggctgccga gccggaaatc
      961 gaacaggcct acggaaaagg tttgcccaat ggcaaattcg agggccgact ccgggtcggc
     1021 aaagcccttc accttatagc caacgtcagc gagaagttcc gccgtggtgg tcagcaggtc
     1081 gatgtcatcg tccagaatca ggacgtcgac atgcttgtca gtgtttgtca ttcttcacgg
     1141 ccttgcccga accaagcgtt tgcatagact tgattatccg gcagaagctt tacaattcac
     1201 taaattgatt aactacgaat cggtaaacag cggcagcgta tctaccttgt tcaactgtgg
     1261 attgagcggc cagcaaccgc catggcggcc tctttcaccg cttcagacat cgtcgggtga
     1321 gcatggcaag tgcgggctag gtcctcggcg gaaccgccaa actccatcaa aacagcgatt
     1381 tcgtggatca attcgcccgc caccggacca atgatatggg cgcccaaaac ccggtcggtg
     1441 cgggcatcag cgaggatttt gacaaaacca tcggtggtct ggttggcctt ggccctgcca
     1501 ttggcaagga aaagaaattt acctgccttg tagtcaacgc cagcagcctt gagttcttct
     1561 tcggtcttgc ccacggcagc aacttcaggc gtggtataga ccacgccggg gatgacatca
     1621 tagttcacat ggcccgcctc accagccagc aattcggcca gcgccacgcc ttcgtcttcg
     1681 gccttgtggg ccagcatggg ccccctgatc acgtcgccga tggcgtagac gccctcgagg
     1741 ctggtttcat aatggccgcc cacttcgatg cggcccctgt catcgcgctt gatgccggcc
     1801 ttttcacagc ccaggccctc ggtgaagggc gtgcggccca ccgccaccag taccgtatcc
     1861 gcttcgaggg attcagcggc accgcccgta ttgggttcaa tctgcagggt gacgcctgat
     1921 ttcgctttcg tggcgctggt caccttgctg gagagcttga aggtgaagcc ctgcttttgc
     1981 agcaagcgct ggaagttctt tgcgacttcc agatccatcc cgggcaggat gcggtcaaga
     2041 tattcaatca cggtaatttt tgcgccgagc ctggcatata ctgagccgag ttccaggccg
     2101 atgacgccgg cgccaatgat ggcgagatgg gtgggcactt tggcaagctc cagggctccg
     2161 gtagacgaga cgatttgctt ctcatcgatc tcgatgccct tgagcttcgc cacatcggag
     2221 cctgtggcga tgacgatgtt tttcgtttcg acgatctggc ccgcgacttc gatcttgccg
     2281 gtgccaagga tcttgcccgt tccgcgaatg accgtgatct tgtttttctt catcagaaaa
     2341 tcaatgccct tggtattgcc gtcaatggct tcctgcttga agaccatcat cttcggcagg
     2401 tcaaagctgg gtgagacggc gatgcccatt tttgcgaacg agtgcttggc ctcggcaaaa
     2461 agctctgagg cgtggagcag ggccttggac ggaatacagc cgatgttcag gcaggtgcca
     2521 ccatgggcag cgcgcttttc gatgaccgcc actttcatgc cgagctgggc ggcgcggatg
     2581 gcgcagacat agccgcccgg gccggtgccg attacggtga gatcaaattg ttcagccatg
     2641 gcgggctcct caaaatttca aatactgcgg ccacctgaaa cacccaggca cgttccggta
     2701 acataggatg ctttttcgga tgccagccag ataattgcct cggctacctc gagtgacgtg
     2761 ccttcgcgct gcatgggaag ctgcccacgc atctgggcga cacggtccgg ttcgccgccg
     2821 gaggcgtgga tatcggtgtc aatgattccg gggcgaaccc cattcacgcg gattccctca
     2881 cttgccactt cgcgggcaag gcccagggta aagacatcga tggcgccttt gctggcagca
     2941 taatcgacaa acatgccggg ggcaccaaga gttgccgcgg ctgaagacag attgacaatt
     3001 gcgccgccgg ttccgccatg gcgcgtggac atgcgcttca ccgcttcgcg ggcgcagagg
     3061 aaggaacccg tgatgttgat ggcgaacatg cgcgccagcc tttcacccga catttcgtcg
     3121 accctggcct tcgtgtcgac tacgcccgca ttattgacca gcactttcaa ttgaccgagc
     3181 tggtcggctg atttgaacag cgccacgatg tcgttttcac tggccacatc gccgtggatt
     3241 gccgtggcct tgccgccggc tttttcaatt ttcgcaacaa cagatttggc cgccgcagcg
     3301 ttcgaaacat agtttaccac aacgaggtaa ccggcttttg ccagcaacag gcaggtggca
     3361 gcgccgatac cacggcttcc gccggttacg atggcgacat caggcttctg ggccatggac
     3421 cgcgacccta caaatccaac agggcgcgct gcgggtcttc gaggctttcc ttgacgcgca
     3481 ccaggaaagt gacggcttcc ttgccgtcga caatgcgatg gtcataggag agcgccagat
     3541 acatcatcgg gcggatcacc acctggccgc caagcgccac ggggcggtcc tggatcttgt
     3601 gcatgccgag aatgccggat tgcggtgcgt ttaggatcgg cgttgacatc agggagccgt
     3661 aaacaccgcc atttgagatg gtgaaggtgc cgccctgcat gtcggcgatc tgcagctggc
     3721 cgtcgcgggc ctttttgccg aagtcggcta cggtcttttc aattgttgca aaagacatgc
     3781 ggtcggcatc gcggaccacg ggaaccacaa ggcccttctc ggtgcccacg gcgatgccga
     3841 ggtggtagta gttcttgtaa accagatcat cgccgtcaat ttcggcgttg acggcaggca
     3901 catccttcag ggcctggatc acggccttgg caaagaagct catgaagccg agctttacgc
     3961 catgcttttt ctcaaacaca tccttgaagg aattgcgcag cgtcatcacg gcgctcatgt
     4021 ccacctcgtt gaaggtggtg agcatggcgg cggtattctg ggcttccttc aggcggcggg
     4081 cgatggtgag gcgcagcctc gacattttaa cacgctcttc gccacccgag ggcgcggggg
     4141 cggcggccgg caggctggcc ggcgcggcag cgggcctggc caattgctcg atgacatcgc
     4201 ccttggtcaa gcggccatcc cgcccggtgg cgggaatggt ggcagggtcg agcttgttct
     4261 cggtgacgat cttgcgcaca gcaggggaaa ggggcgcttc cgcctttaca ggttctgcga
     4321 tctttgcggg agcaggtgcg gcaacgggag ccgccgctgc cggcttggct gcggccccgg
     4381 cggcgccttc ttcaatggcg ccgagcaagg caccgacatt gaccgtggtt cctgcggcgg
     4441 cggcaattga tgtgagcttg ccggaagctg gggccggcac ttccaccgtt accttgtcag
     4501 tttcgagttc gaccatgggc tcatcggcct tcaccgcgtc gccgacattc ttaaaccatt
     4561 ttgctattgt cgcttcagtg acagattcac cgagggcagg cacgcgaatt tccttagcca
     4621 ttcttttctt tcctcaatct ctttcagccc agggccactt caaggaaatc cttcaattcc
     4681 ttgatatgcc tgctcatcaa tccggtggcg gtggaagctg acggattgcg ccctgcatac
     4741 ctggcttttg cgttcgttgc cttcaggtaa tccagaaccc ggtcaatgtt gggctgggcg
     4801 taggtccagg ctcccatgtt catgggttct tcctggcacc agacaaattc ggctgatttg
     4861 aaacggacga gttcctggat cagggccttg tgcggccagg gatagagctg ctcgacgcga
     4921 agaagataaa tatcggttag gccgcgcttt tcgcgctcct cgtagagatc gaaatagacc
     4981 ttgccggaac acatgaccac ccggcggatt ttatcgtcat tgacgagctg gataggctgg
     5041 tccttcaatg tttgggcatc atcccaaaga atgcggtgga atgaggtgcc ttcggcaaac
     5101 tcctccagcc gcgaggtggc gcgcttgttc ctgagcaggg acttcggcgt catcatgatc
     5161 aggggcttgc ggatgtcgcg cttcagctgg cggcgcaaga tgtggaagta gtttgccggc
     5221 gttgtgcaat tggcaacctg catgttgtcc tcggcgcaca tctgcaggaa gcgctcgagt
     5281 cttgcggaag aatgctccgg cccctgaccc tcataaccgt gcggcagcag gcagacgagg
     5341 cccgacatgc gcagccattt gcgctcaccg gacgatatga actggtcgaa gaccacttgc
     5401 gcgccattgg cgaaatcgcc gaattgcgct tcccagaggg tgagggtgtt gggctcggcc
     5461 agtgagtagc cgtactcgaa gcccagcacg gcctcttccg agagcatgga attgatgact
     5521 tcgatcttgt gctgctggtc cttggcgata tggttgagcg gcgtgtagaa cttgccggtc
     5581 aactggtcga cgagaaccgc gtggcgctgc gagaaagtac cgcgctgcac gtcctggcca
     5641 gacagcctga cccggaaacc atcattgagg agggtggcga aggcgaggtt ttcgcccatg
     5701 gaccagtcaa gacccacgcc ctcttcaatc atcttgtggc gggcatcgag cacgcgctgc
     5761 atggtcttgt gcactgagaa ggttttcgga gccttggtga tcttcagtcc aaggtctttc
     5821 agggtcttga ggtctacgcc ggtcgaacct tttcgcgggt cgtccagatc cttggctgat
     5881 ttcatgcccg cccagcggcc atcgagccag tcggccttgt tggccttgta gccttccgat
     5941 gcggcaaaat cctcttttat gttcatgcag taagcggcca ccaagatcta caatccttcc
     6001 ctacacgacg cgcttcgctt
//
LOCUS       DAORVN010000019         6019 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_8620_length_6019_cov_3.421697, whole genome shotgun sequence.
ACCESSION   DAORVN010000019 DAORVN010000000
VERSION     DAORVN010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 6019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6019
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8620_length_6019_cov_3.421697"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..355)
                     /locus_tag="PKV23_00705"
     CDS             complement(<1..355)
                     /locus_tag="PKV23_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018064506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HQX83978.1"
                     /translation="MSRFIEFEGGDVESYLKTQENKDLLRFITCGSVDDGKSTLIGRL
                     LYEAKMLFEDQLLALEADSRKMGTQGGNLDFALLVDGLEHHVLDDRQARRERQALAHA
                     VGAQAQRARVGLDRSV"
     gene            complement(355..1275)
                     /gene="cysD"
                     /locus_tag="PKV23_00710"
     CDS             complement(355..1275)
                     /gene="cysD"
                     /locus_tag="PKV23_00710"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019169787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HQX83979.1"
                     /translation="MPQAPVPRKLTHLERLEAESIHIIREVAAECEKPVMLYSVGKDS
                     AVMLHLAMKAFYPAKPPFPLMHVDTRWKFRDMISLRDETAKRLGLDLIVYINPDGVEK
                     DVNPFDHGSALHTQIMKTDGLKQALDKYGFDAAFGGARRDEEKSRAKERIFSFRDTRH
                     RWDPKNQRPELWNLYNARKNKGESIRVFPLSNWTELDIWQYIHIENIPIVPLYFAAPR
                     PIVWRDGQMIMVDDERMRLNPGEKPELRNIRFRTLGCYPLTGAIDSNATSLLDIIEEM
                     LVATSSERQGRVIDKDQAASMEKKKQEGYF"
     gene            1408..2043
                     /locus_tag="PKV23_00715"
     CDS             1408..2043
                     /locus_tag="PKV23_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008835932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HQX83980.1"
                     /translation="MAKKGVPKLPYQEPHLGAAARGKLLEEAIGREVRGFREKLGITI
                     SELAKAADMSAGMLSKIENGATSPSLGSLQALGKALQVPVTSLFRGFEEIRDASFVKA
                     GQGLTIERRGTRAGHQYQLLGHSPHGAVMVEPYMITLTAQSDVFPTFQHSGLEMLYML
                     EGNVVYRHGDKTYDMHPGDTLFFDADVPHGPEELKKLPARYLSVISYRRDD"
     gene            2143..3045
                     /locus_tag="PKV23_00720"
     CDS             2143..3045
                     /locus_tag="PKV23_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018697654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase family protein"
                     /protein_id="HQX83981.1"
                     /translation="MCGIVGLYLKNPKLKSKLGKMFKPMLIEMTNRGPDSTGVAVYRN
                     PVKKAQTKFSLAHDDANYDWKKIDVGLEKALKCDCSISKIDSHCILVTTAKEAAVIAW
                     LKAHHPGVRVVGTGHTIEIFKETGLPAEVYERFSLDEAQGTHIIGHTRMATESAVTTA
                     GSHPFATGADLCLVHNGSLSNHNRLRESLRKEGMDFQTENDTEVAAAYMTSKLRGGAT
                     LKEALEDGLRDLDGFYTFLVGTADGFAVVRDPIACKHAVMAETDDWVAMATEYRAIAH
                     LPGADTAKIWEPEPTIVYTWGGHA"
     gene            3042..3725
                     /locus_tag="PKV23_00725"
     CDS             3042..3725
                     /locus_tag="PKV23_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein GlxC"
                     /protein_id="HQX83982.1"
                     /translation="MNELDLHKIGVRGINSQLHSLPKNTNEKHWVIHNPKGQHSIAVG
                     LDAPLQIDIRGHVGFYCGGMNKDAELTIYGHAGVGTAENMMSGVIWVKGNSSESAGAT
                     GNGGLLVIDGDASSRCGISMKGIDIVVGGSVGHMSAFMAQRGNLVVCGDAGDALGDSI
                     YEARLYVRGKVASLGADCIEKEMRPEHLADLERLLKAANRKEKPSEFRRYGSARKLYN
                     FSVDHADAY"
     gene            3733..5058
                     /locus_tag="PKV23_00730"
     CDS             3733..5058
                     /locus_tag="PKV23_00730"
                     /EC_number="1.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014332432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HQX83983.1"
                     /translation="MDKTPRTLPRKSATFDDYTLSEIRRAASTGIYDIRGGGAKRRLP
                     HFDDLLFLGASISRYPLEGYREKCGTNVVLGTRFAKKPIELKIPITIAGMSFGSLSAQ
                     AKEALGRGATIAGTSTTTGDGGMTPEERGQSKTLVYQYLPSRYGMNPDDLRKADAIEI
                     VIGQGAKPGGGGMLLGQKISDRVAAMRCLPKGIDQRSACRHPDWTGPDDLEIKINELR
                     EITNWEKPIFVKIGGARPYYDVALAVKAGADVVVLDGMQGGTAATQEVFIEHVGQPTL
                     ACIRPAVRALQELNMHRKVQLIVSGGIRNGADVAKALALGVDAVSIGTAALVALGDND
                     PHFETEYNELGTTAGSYDDWHEGRDPAGITTQDPELAKRLDPVKAGRRLSNFLKVMTL
                     EAQTIARANGKSHVHNLEPEDLCALTVEAAAMAQVPLAGTDWIPGRGGY"
     gene            5084..5695
                     /locus_tag="PKV23_00735"
     CDS             5084..5695
                     /locus_tag="PKV23_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008834620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HQX83984.1"
                     /translation="MEITVLAIFAGALFINSSSPGPSVAALVARVLTSGWRSVMPLLA
                     AMWIGEALWLTTAVFGLSVMAQTFYWAFVILKYLGVAYLLYLAYRMWTAPSVVKDGAL
                     PAGDSSWRLFLTGMAITLGNPKIMVFYVALLPSIVDLARISLMGWMELTVTMVAIIVA
                     VDLTWVMIAAQARRWLKSPRAVRIANRTSATAMGGAALVIATR"
     gene            5728..>6019
                     /locus_tag="PKV23_00740"
     CDS             5728..>6019
                     /locus_tag="PKV23_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011440034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III glutamate--ammonia ligase"
                     /protein_id="HQX83985.1"
                     /translation="MAGELEAYAKKNKVRFFLFNFTDLFGTQRAKLVPAEAATRMQKA
                     GAGFAGFATWLDQTPAHPDILVMPDPDAIIPLPWRPEVAWVAGDPWMSGKPLE"
BASE COUNT         1242 a         1639 c         1915 g         1223 t
ORIGIN      
        1 gtaccgaccg gtcgaggccg acgcgcgcac gctgcgcctg cgcgcctacc gcgtgggcca
       61 gcgcctgccg ctctcggcga gcctgccgat cgtcgagaac atggtgctcg aggccatcga
      121 cgaggagggc aaaatcgaga ttgccgccct gagttcccat tttcctggaa tcggcttcca
      181 aagcgaggag ctggtcctca aacagcatct tggcctcgta gaggaggcgg ccgatgaggg
      241 ttgattttcc gtcgtcaacg gaaccgcagg tgatgaagcg caggagatcc ttgttctcct
      301 gggtcttcag gtaggattcg acatcgccac cttcaaattc gataaaacgg gacatcagaa
      361 atatccctcc tgcttcttct tctccatgga ggcggcctgg tccttgtcga tgacgcggcc
      421 ctggcgttcg gaagaggtgg cgaccagcat ttcctcgatg atgtcgagaa gcgaggttgc
      481 atttgaatca atggcaccgg tcaggggata gcagccgagg gtgcggaaac ggatgttgcg
      541 caattcaggt ttttcgccgg ggttgagccg catgcgctca tcatccacca tgatcatctg
      601 gccgtcgcgc cagacgatgg ggcggggggc agcgaaatag agcggcacga tggggatgtt
      661 ctcgatgtgg atatattgcc agatgtcaag ttcggtccag ttcgagagcg ggaacacgcg
      721 gatcgattca cccttgttct tgcgggcgtt gtagaggttc cacaattcgg ggcgctggtt
      781 cttcggatcc cagcgatgcc tggtgtcgcg gaaggaaaaa atgcgctcct tggcgcggga
      841 tttttcctcg tcgcggcggg ccccgccaaa ggcggcatcg aagccatatt tgtcgagggc
      901 ctgtttcagg ccgtcggttt tcatgatctg ggtatgcagc gccgagccat gatcgaaggg
      961 gttcacatcc ttctcgacgc catcaggatt gatatagacg atgaggtcaa gccccaggcg
     1021 cttggcggtt tcatcccgga gggaaatcat gtcgcggaat ttccagcggg tatccacatg
     1081 catcagggga aagggcggct tggcgggata aaaggccttc atggcgaggt gcagcataac
     1141 ggctgaatcc ttgcccaccg aatagagcat tacgggcttt tcgcattcgg cggcgacttc
     1201 gcggatgatg tggattgatt ccgcctcgag gcgttcgaga tgggtcagtt tcctggggac
     1261 tggggcctgc ggcattacgc tatccttgtt cgggcgggtt tatggcggtg tcctcgcgca
     1321 aaggcaaggc gggacttgcc cccatatggg cttcgggtta gtgcttaaag agagaagcta
     1381 atgaaaaatc catcaccaaa aaagcctatg gccaagaagg gagtgcccaa acttccctac
     1441 caggaaccgc atctgggtgc ggcggcgcgg ggcaagctgc tggaggaagc catcgggcgc
     1501 gaagtgcgcg gtttccgtga aaaactaggc atcaccattt cggaactggc caaggcggcg
     1561 gacatgtcgg cgggcatgct ttcaaaaatc gagaatggcg ccacctcgcc ctcgctcggc
     1621 tcgctgcagg ccctgggaaa ggcattgcaa gtgccggtca cttcgctgtt ccggggtttc
     1681 gaggaaatcc gcgatgcttc cttcgtgaaa gccggacagg ggctgaccat tgaacggcgc
     1741 ggcacccggg cgggccacca gtatcagttg ctgggccaca gcccccatgg ggcggtcatg
     1801 gtcgaaccct atatgatcac gctcacggcc cagtcggatg tgttccccac cttccagcat
     1861 agcgggcttg agatgctcta catgctcgag ggcaatgtgg tgtaccgcca tggcgacaag
     1921 acctatgaca tgcaccccgg cgatacgctg ttttttgatg ccgatgtgcc ccatgggccc
     1981 gaggagctga aaaagctccc ggcgcgctat ctgagcgtaa tcagctaccg gcgggacgat
     2041 taaccccctc attcctcttg tgaaaaaata attcctagta gttgataatg ccgtaggggc
     2101 ctgatacagt ctttcaacgc tgtttcagga gaggtttttg ccatgtgcgg cattgtcggt
     2161 ctttatctca agaatcccaa attgaaatcc aaactgggca aaatgttcaa gccgatgctc
     2221 atcgagatga ccaatcgcgg gccggattca actggcgtcg cggtgtaccg caatccggtg
     2281 aaaaaggccc agacgaaatt ttccctggcc catgacgatg ccaattatga ttggaagaaa
     2341 attgatgtgg ggctggaaaa ggccctgaag tgcgattgtt cgatcagcaa gatcgacagc
     2401 cactgcattc tggtgactac ggccaaggaa gcggcggtga tcgcgtggct caaagcccat
     2461 catcccggcg tgcgcgtggt gggcacgggc cacaccattg aaattttcaa ggaaacgggc
     2521 ctgccggcgg aagtttatga gcggtttagc ctggatgaag cgcagggcac gcacatcatc
     2581 ggccacaccc gcatggcgac agagagcgcg gtgacaacgg ccggctcgca tccctttgcc
     2641 accggggctg atctttgcct ggtgcataac ggttcgctct ccaatcataa ccgcctgcgc
     2701 gaaagcctgc gcaaggaggg catggatttc cagacagaga acgacacgga ggtggccgcc
     2761 gcctacatga cctcaaagtt gcgcggcgga gccacgctga aggaagcgct ggaagacggg
     2821 ctgcgtgatc tcgatggctt ctacacgttt ctggttggaa ccgccgacgg atttgccgtg
     2881 gtgcgcgatc ccatcgcctg caagcatgcg gtgatggcgg agaccgatga ctgggtggcg
     2941 atggcgactg aataccgcgc cattgcgcat ttgcccgggg cagacacggc aaaaatctgg
     3001 gagcccgaac ccaccatcgt ttacacttgg ggcggacacg catgaacgag cttgatcttc
     3061 acaagattgg ggtgcgggga atcaactcgc agcttcacag cctgcccaag aacaccaatg
     3121 agaagcattg ggtcattcac aatcccaagg gccagcattc cattgcggtg ggccttgatg
     3181 caccgttgca gatcgatatt cgcggccatg tgggcttcta ttgcggcggc atgaacaagg
     3241 atgcggaatt gacaatttat ggccatgcgg gcgtggggac ggctgaaaac atgatgtccg
     3301 gcgtgatctg ggtgaagggc aattcatcgg aatccgcagg cgctacgggc aatggcggct
     3361 tgctggtgat cgacggcgat gcttcctcgc gctgcggcat ttccatgaag ggcattgata
     3421 ttgtggtcgg cggttcggtt ggccacatgt cggctttcat ggcgcagcgc ggcaatctgg
     3481 tcgtctgtgg cgacgcgggc gatgcgctgg gcgattccat ttacgaggcg cggctctatg
     3541 tgaggggcaa ggtagcaagc ctgggggccg attgcatcga gaaggaaatg cggcctgaac
     3601 atctggccga tcttgagcgc ctgctgaagg ctgccaaccg caaggagaag ccatcggaat
     3661 tccggcgcta cggctcggcc cgcaagcttt acaatttcag cgtcgatcac gcggacgcat
     3721 actgaggaca ccatggacaa aaccccccgc acgctgccgc gcaagtcagc aacctttgac
     3781 gactacacgc tttctgaaat ccgccgggcg gcgagcaccg gcatttatga tattcgaggt
     3841 ggcggcgcca aacgcaggct gccgcatttt gatgacctgc tgtttttagg agcttccatc
     3901 tcgcgctatc cgctggaggg ctaccgggaa aaatgcggca ccaatgttgt gctgggaaca
     3961 cgctttgcca agaagcccat tgaactgaaa attccgatca ccattgcggg catgagcttt
     4021 ggttcgctct cggcgcaggc caaggaagcg ctggggcgag gagcaaccat tgccggcacg
     4081 tcgacaacga cgggcgatgg cgggatgacg ccggaagaac gcgggcaatc caagacgctg
     4141 gtttatcagt atctgccctc gcgctacggg atgaacccgg atgatctgcg caaagccgat
     4201 gccattgaaa tcgtcatcgg ccagggcgcg aaacccggcg gcggcggcat gctgctgggc
     4261 cagaaaattt cggaccgtgt ggcggccatg cgctgcctgc ccaagggcat cgaccagcgt
     4321 tcggcctgcc gccatccgga ctggacgggg cctgatgacc ttgaaatcaa gatcaatgaa
     4381 ctccgcgaga tcaccaattg ggaaaagccc atctttgtga agatcggcgg ggccaggccc
     4441 tattatgacg tggcgctggc agtgaaggcc ggagccgatg tcgtggtgct ggacggcatg
     4501 cagggcggca cggcggccac ccaggaagtg ttcattgaac atgtgggcca gccgacattg
     4561 gcctgcatcc gcccggcggt tcgcgccttg caggagctca atatgcaccg caaggtgcag
     4621 cttattgtgt ccggcggcat ccggaatggc gctgatgtgg ccaaggccct ggcgcttggg
     4681 gtggatgcgg tgtcgattgg cacggcggcc cttgtggcac tcggcgacaa tgatccgcat
     4741 tttgaaacgg aatacaatga actcggcaca acggctggct cctatgacga ctggcatgag
     4801 gggcgcgatc ccgccggcat cactacgcaa gatcccgaat tggccaaacg gcttgatccg
     4861 gtgaaggcag ggcgcaggct ttccaacttc ctgaaggtaa tgacactgga ggcgcagacc
     4921 atcgcccggg ccaatggcaa atcccatgtc cacaacctcg agccggaaga cctgtgcgcg
     4981 ctgacggtgg aagccgcggc catggcgcag gtgccgcttg caggcacgga ctggattccg
     5041 ggccggggcg ggtattagga tggggatttc ttaggagacc attttggaaa tcaccgtcct
     5101 cgccatattc gccggagcac ttttcataaa ttcgagttcg cccgggccaa gcgttgcggc
     5161 cttggtggcg cgggttctga ccagcggctg gcgcagtgtc atgccgctgc tagccgccat
     5221 gtggattggc gaggcgctgt ggctgacaac ggcagtgttc ggcctcagcg tcatggcgca
     5281 aaccttttat tgggcttttg tgatcctgaa atatctcggc gtggcctacc tgctatatct
     5341 ggcttaccgg atgtggaccg cgccttctgt ggtcaaggat ggggcgctgc cagcggggga
     5401 ttcttcatgg cggctgtttc ttaccggcat ggcgatcacg ctgggcaatc cgaaaatcat
     5461 ggttttctat gtggcgctgc tgccctcaat cgttgacctc gcgcgcattt cgttgatggg
     5521 atggatggag ctcacggtca ccatggtggc aattattgtg gctgtcgatc tcacctgggt
     5581 gatgattgcg gcgcaggcgc ggcgctggct gaagagcccg cgcgccgtgc gcatcgccaa
     5641 ccggacaagc gccacggcca tgggcggggc agcgctcgtg attgcaacaa gatgaaacct
     5701 aataatgata accgggagtg gaaataaatg gctggggaac tcgaagcata tgccaagaag
     5761 aacaaggtaa ggttcttcct gttcaatttc accgacctgt tcggcacgca gcgcgccaag
     5821 ctggtgcctg ccgaagcggc aacacggatg cagaaagcgg gtgccgggtt tgcggggttc
     5881 gccacctggc ttgaccagac accggcgcat ccggacatcc tcgtgatgcc tgaccctgat
     5941 gcgatcattc cattgccgtg gcggccggaa gtggcctggg ttgcgggcga tccgtggatg
     6001 agcggcaagc ctttggaac
//
LOCUS       DAORVN010000020         6012 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_8632_length_6012_cov_3.877623, whole genome shotgun sequence.
ACCESSION   DAORVN010000020 DAORVN010000000
VERSION     DAORVN010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 6012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6012
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8632_length_6012_cov_3.877623"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..1032
                     /locus_tag="PKV23_00745"
     CDS             1..1032
                     /locus_tag="PKV23_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007606072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HQX83986.1"
                     /translation="MLFTAALLAGTAFAASARAGDELTVTSWGGSYSESQRKAYYEPY
                     MAAGNKITEAEYNGEVAKIKAMVEANAITWDVIDVDTATALQGCAEGALETIDWAKLG
                     LDRSKFIGAELMECAVPTIVYGTVFGYDTSKLAEGPTTINDFFDLAKFPGKRGIQKNP
                     TVNLEWALIADGVDAKDVYTTLSTPEGVDRAFKKLDTIKSEIVFWEAGAQAPQLLADG
                     QVVMVSAWNGRLQTAIDKDKKPFKIIWDHQGLDWDWWAITKGTPKIEEAYRFVAFASG
                     VGPQAEQTKYIAYGPANKDAIPGIEPATLPNLPTAPDNLKTAFNVDPQFWADHGDELK
                     ERFNAWLAK"
     gene            1093..2361
                     /locus_tag="PKV23_00750"
     CDS             1093..2361
                     /locus_tag="PKV23_00750"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018640981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQX83987.1"
                     /translation="MTDDVMRTADGTPLKVKLRQAERREVRRAFLLIAPLFLFIVVSF
                     LIPIVVMLKNAFYDPDIVNNLPQTTELLRQWDGKALPSEEVFAAFAADLKTASKARTV
                     ALIGKRLNYEIPTLKSKVTVTARKLDKIEAGPWTEAVIAADPIWKDVFTWAVVKRAGS
                     AVTPYYLLSALDLRQGTDGGIERTPEERRIYLQVLGRTLGISLLVTLCTLVLGYPVAF
                     MLATLPQRTASILMIFVLLPFWTSLLVRTTAWTILLQDGGVVAQFLHITGITWISNVL
                     GFMSGEPQLFKTRFATVLAMTHIQLPFTLLPIYSVMKTISPTYMRAAKSLGATPFHAF
                     RTVYVPQTMPGVAAGCLLTFILCLGYFITPALVGGGSDNMIAGFIDTAMNNEGNWGKS
                     SALGFVLLTATLILYYVYNRLIGIDKMKLG"
     gene            2368..3204
                     /locus_tag="PKV23_00755"
     CDS             2368..3204
                     /locus_tag="PKV23_00755"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007600386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQX83988.1"
                     /translation="MALPPYMTTTEKVSHYGLRIFVGAVLLFLIAPILAIMPLSFNSE
                     TFFSYPMPGFSTKWYFGETGFFTNERWTSALKLSVIVAVAATILATCLGTLAALGLSR
                     SNIPGRAAIMAVLISPMIVPVIISAIGLFFFYAYTGLNGTLIGIILAHTALATPFVVI
                     TVTATLMSFDRTLMRAGASLGGAPHTVFFKVVLPLILPGVISGALFAFVTSFDEVIVI
                     LFIGGPEQRTLPRQMFSGIRELISPTITAAASVLIVFSTFLLVTVELLRRRGERLRGI
                     RS"
     gene            complement(3201..3395)
                     /locus_tag="PKV23_00760"
     CDS             complement(3201..3395)
                     /locus_tag="PKV23_00760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX83989.1"
                     /translation="MADSAPVGRLFASIATLSDMSNQIGQSLAADDLPGAAHLMKAYA
                     KPASLAETATPLPMHFKRRA"
     gene            3629..4375
                     /locus_tag="PKV23_00765"
     CDS             3629..4375
                     /locus_tag="PKV23_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012066860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HQX83990.1"
                     /translation="MAGHSQYKNIMHRKGRQDKLRSNAFAKLGREITVATKAGLPDPA
                     MNARLRLAVQNARAGNMPWDTIERAIKKASGVDAENFDAMRYEGYGPGGVAIVVEALT
                     NNRNRTASNVRALFTKYGGNMGETGSVSFMFSKVGEISYPLAKGSADALLEAAIDAGA
                     DDAVSDENGHVITCAFENIGEVSASLAKKLGDAESVKVVWKPQTLTPLDQEKAETLLK
                     LVDALDEDDDVQNVFSNADIPDEVMAKLDA"
     gene            4368..4877
                     /gene="ruvC"
                     /locus_tag="PKV23_00770"
     CDS             4368..4877
                     /gene="ruvC"
                     /locus_tag="PKV23_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007765252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HQX83991.1"
                     /translation="MPKVRIIGIDPGLRNTGWGVIEAEGMRLSYVADGSVHSAADAPL
                     ATRLLQIHSQLAEILQRFAPDEAAVEETFVNKDARATLKLGQARGAAMLAPALAGISV
                     AEYAPNVVKKSVVGAGHAEKDQVKHMVKVLLPRAELNSADSVDALAIAICHAHHRGAQ
                     TLAKILARS"
     gene            4936..5553
                     /gene="ruvA"
                     /locus_tag="PKV23_00775"
     CDS             4936..5553
                     /gene="ruvA"
                     /locus_tag="PKV23_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012169046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HQX83992.1"
                     /translation="MIGKLKGRIDSFGTDWLIIDVSGVGYHVFCSSKTLAALPSVGEF
                     AELHTEMLVSQDMIRLVGFASVLEREWFRLLQTVQGVGTRVALAILSTLSASEIANAI
                     ALQDKPMIGRAPGVGKKLAERIVLELKDKAPAFTGADATLAKLQAELEAPRPTSASDA
                     VSALVNLGYGQSQAGSAVAAAMRKNGDDQPTEKLIRQALKELALL"
     gene            5550..>6012
                     /locus_tag="PKV23_00780"
     CDS             5550..>6012
                     /locus_tag="PKV23_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008834631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HQX83993.1"
                     /translation="MNQRLIDREDRPEDGLDSHVRPQSLNEFVGQTKVKKNLRVFIEA
                     AKARAEALDHVLFAGPPGLGKTTLAQIMARELGVNFRATSGPVIAKAGDLAALLTNLE
                     DRDILFIDEIHRLSPAVEEILYPAMEDYQLDIMIGEGPAARSVRIDLAKFTL"
BASE COUNT         1220 a         1690 c         1835 g         1267 t
ORIGIN      
        1 atcctattca cggccgcttt gcttgcggga acggcctttg cggcatccgc ccgggctggc
       61 gacgaactta cagttacgtc atggggtggt tcttattcag agagccagcg caaggcctat
      121 tatgagccct atatggcggc tggcaacaag atcaccgagg ccgaatacaa tggcgaagtg
      181 gccaagatca aggctatggt cgaagccaat gccatcacct gggacgtgat cgacgttgat
      241 accgcgacag ccctgcaggg ttgtgccgaa ggagctctcg agaccatcga ctgggcaaag
      301 ctcgggcttg accgctcaaa gttcatcggc gccgaactga tggaatgcgc tgttcccacc
      361 atcgtgtacg gaaccgtatt cggttacgac acttcgaagc tcgccgaagg cccgacgacc
      421 atcaacgact tcttcgatct tgccaagttc ccaggcaagc gtggcattca gaagaacccc
      481 acggttaatc tcgagtgggc attgatcgcc gatggcgttg atgccaagga tgtttatacc
      541 acgctttcaa cacctgaagg cgttgaccgc gccttcaaga agctcgacac catcaagagc
      601 gagatcgtct tttgggaagc cggcgcccag gcaccgcagc ttcttgccga tggccaggtt
      661 gtaatggtct cggcctggaa cggccgcctg cagacagcga tcgacaagga caagaagccc
      721 ttcaagatca tctgggacca tcagggcctg gattgggact ggtgggctat caccaaggga
      781 acacccaaga ttgaagaagc ttatcgtttt gtagcgtttg cttccggtgt tggtccccag
      841 gctgagcaga ccaagtatat tgcttatggc cctgccaaca aggacgccat tcctggcatc
      901 gagccggcaa ccctgccaaa cctgccaacg gcaccagaca acctcaaaac ggccttcaac
      961 gtggacccac agttctgggc tgaccacggc gatgagctga aagagcgctt caacgcctgg
     1021 ctcgcgaagt aagacatccg gggcggaagg caacttccgc cccgattctt ttccgatgat
     1081 ggggcgttgg cgatgactga tgacgtgatg cggacggcgg acggcacgcc gctgaaggtc
     1141 aaattgcgcc aggcggagcg ccgtgaagtg aggcgggcat tcctgctgat tgcgccgctg
     1201 ttcctgttca ttgtcgtttc gttcctcatc cccatcgtgg tcatgctgaa gaacgccttc
     1261 tatgatccgg acatcgtcaa caatttgccg cagaccaccg aactgctgcg ccaatgggac
     1321 ggcaaggcgc ttccaagcga agaggtgttt gcggcctttg cggcggacct taaaaccgcc
     1381 agcaaggcca gaaccgtggc gctgatcggc aagcggctga actatgaaat ccccacgctc
     1441 aaatccaaag ttaccgtcac ggcgcgaaaa ctcgacaaga tagaagcggg cccatggacg
     1501 gaagccgtca ttgcagcgga tcccatttgg aaagatgtgt tcacgtgggc ggtggtgaag
     1561 cgggcaggca gcgccgttac gccatattac ctgttgagcg cattggacct gaggcagggc
     1621 acggacggcg gcattgaacg cacgcccgag gagcgccgca tatacctgca ggtgctgggc
     1681 cgcacacttg gcatcagcct gctggtgacc ttgtgcacgc ttgttctcgg ctatccggtt
     1741 gccttcatgc tggcaaccct gccgcagcga accgccagca tcctgatgat attcgtgctg
     1801 cttcctttct ggacttcgct tcttgtgcgc accactgcct ggaccattct gctccaggac
     1861 ggcggtgtgg ttgcgcagtt cctgcatatc acgggcataa catggatctc caatgtgctc
     1921 gggttcatgt caggcgagcc gcaactgttc aagacccgct ttgccacggt gctggccatg
     1981 acgcacatcc agttgccgtt cacgctgctg cccatctaca gcgtgatgaa aaccatatcg
     2041 cccacctata tgcgcgcggc gaagtcattg ggcgccacgc cgtttcatgc tttccgcacg
     2101 gtctatgtgc cgcaaaccat gcccggcgtg gcggcaggct gcctgctgac cttcattctc
     2161 tgtcttggct acttcatcac gccggcgctg gttggcggcg gctcggacaa catgattgcc
     2221 ggattcattg acacggcgat gaacaatgaa ggcaactggg gcaagtcgtc tgcccttggc
     2281 tttgtgctgc tcaccgcaac acttattctc tactatgttt acaaccgcct catcggcatc
     2341 gacaaaatga agctgggcta gaagacaatg gcattgcctc cctatatgac gacaaccgaa
     2401 aaagtctctc attatggctt gcggattttc gtgggcgcgg tcctgctgtt cctgattgca
     2461 ccgatcctgg ccatcatgcc gctgtcgttc aattccgaaa cgttcttcag ttatcccatg
     2521 cccgggtttt ctacgaagtg gtattttggc gagaccgggt tcttcaccaa tgagcgctgg
     2581 accagcgcgc tgaagctttc cgtcattgtc gctgtcgccg cgacaattct tgccacctgc
     2641 cttggcacac tggctgctct tggcctttcc cgctccaaca ttcccggccg ggccgcgatc
     2701 atggcggtgc tgatctcgcc gatgattgtt cccgtcatca tttcggcaat cggcctgttt
     2761 ttcttttatg cctataccgg cctgaatggc actctgatcg gaatcatcct tgcccacacg
     2821 gcgctggcca cgcctttcgt ggtcatcacg gtcactgcaa cactgatgag ctttgaccgc
     2881 acgctcatgc gggccggggc ctcgctcggc ggggcgccgc acacggtatt tttcaaagtg
     2941 gtgctgccgc tcatacttcc cggcgtcatt tcgggcgcgc tctttgcctt cgtcacgtcg
     3001 tttgacgaag tgatcgtgat cctgttcatc ggcgggccgg agcagcggac gctgccgcgg
     3061 cagatgttct cgggcatccg cgaactgatt tcgcccacca tcacagccgc ggcaagtgtg
     3121 ctgatcgtgt tttcaacctt cctgcttgtg acggttgaac tgctgcgccg ccggggcgaa
     3181 cgcttgcggg gaatcagaag ctaggcgcgg cgcttgaagt gcatcggcag gggcgttgcc
     3241 gtttcagcca ggctggcggg ctttgcgtaa gccttcatca gatgtgcggc accgggaaga
     3301 tcgtcggcgg cgagcgactg gccgatctga ttcgacatgt cggagagggt ggcgatggat
     3361 gcaaagaggc ggccgacggg ggctgaatct gccaagctgc ggacctttcg aagggcgctg
     3421 gaacaggcgc cagcaaggtt gtctttactc gtgaacgaga actcttcgac catacgcgac
     3481 ttgattgtca taaactcaca ctccaccaac tgagacgccc aagttgactt atggccgtta
     3541 atggcaggta aacagactta cgtgcggctt gaatccgcat ggccgccacg gctataccgc
     3601 tggcagatta cagtttcttg agggttttat ggcgggtcat tcacaataca agaacatcat
     3661 gcaccgcaag ggcaggcagg acaaactccg ttccaacgcc ttcgccaagc tgggacgcga
     3721 gatcaccgtt gcgacaaagg caggccttcc ggacccggcg atgaatgcgc gcctgcggct
     3781 tgcggtgcag aacgcccggg cgggcaacat gccctgggac acgatcgagc gggcaatcaa
     3841 gaaggcttcg ggcgttgatg cggagaattt cgatgccatg cgctatgagg gctatgggcc
     3901 tggcggcgtt gccatcgtgg ttgaagcgct gaccaacaac cgcaaccgga cggccagcaa
     3961 tgtgcgggcg ctcttcacca agtatggcgg caacatgggg gagaccggtt cggtgagctt
     4021 catgttctcc aaggtcggtg agatttccta tccgctggcc aagggcagcg ccgatgcctt
     4081 gcttgaggcg gcaatagacg ccggagccga cgatgcagtg agcgacgaaa acggccatgt
     4141 cattacctgc gccttcgaaa atatcggcga ggtttcggcc agtcttgcca aaaagctggg
     4201 cgatgcggaa agcgtgaaag ttgtctggaa gccgcagacg ctgacgccgc ttgaccagga
     4261 aaaggccgag acgctgctca agctggtcga tgcgctggat gaggatgacg atgtgcagaa
     4321 tgtcttcagc aatgcggata ttcccgacga ggtgatggcc aagctggatg cctaaggtgc
     4381 gcatcatcgg cattgacccg ggcctgcgca atacaggctg gggcgtcatc gaggcggagg
     4441 gcatgcggct ttcctatgtg gcagatggca gcgttcattc cgctgcggat gcaccgctgg
     4501 cgacgcggct gttgcaaatt cattcccagc ttgcggagat cctgcagcgc tttgctcccg
     4561 acgaagcggc tgtcgaagag acctttgtga acaaggatgc gcgggcgact ttgaaactgg
     4621 ggcaggcgcg gggggcggcg atgctggcgc cggcactggc ggggatcagc gtcgccgaat
     4681 atgcgcccaa tgtggtgaag aaatccgtgg tgggcgccgg ccacgcggaa aaggaccagg
     4741 tgaagcacat ggtgaaagtg ctgctgccac gcgccgaact caattctgcg gattccgtag
     4801 atgcgctggc cattgccatt tgccacgcgc atcaccgggg ggcgcaaacc ctagccaaga
     4861 ttctggcgcg ttcttgactt gttccagtgg aatgcctagg catgcgatag tgactaagcg
     4921 aaacacgcgg gaatcatgat tggaaagctc aaggggcgca ttgacagctt cggcacggac
     4981 tggctgatca ttgatgtgtc gggtgttggt tatcatgtgt tctgttcatc gaagacgctt
     5041 gcggcgctgc catcggtcgg cgagtttgcc gagcttcata cggaaatgct ggtgagccag
     5101 gacatgatcc ggctggtggg ctttgcctcg gtgctggagc gggaatggtt ccggcttttg
     5161 caaaccgtgc agggcgtggg cacgcgggtg gcgctggcca ttctttccac tttatccgct
     5221 tcggaaattg ccaatgccat tgccttgcag gacaagccca tgattggccg tgcgccaggt
     5281 gttggcaaga aactggcgga gcgcatcgtg ctggaattga aggacaaggc gccggccttt
     5341 acaggtgctg acgctacgct tgccaaattg caggcggagc tagaggcgcc gcgcccaacc
     5401 tcggcttcgg atgcggtgtc ggcccttgtc aacttgggtt acggccaaag ccaggcgggt
     5461 tcggcggttg ccgccgccat gcgcaaaaac ggcgatgacc agccgacgga gaaactgatc
     5521 cggcaggcgc tgaaggaatt ggcactgcta tgaaccaacg cctcatagac cgtgaagaca
     5581 ggccggagga tggcttggac tcccatgtgc ggccgcaatc gctgaatgag tttgtggggc
     5641 agacgaaggt caagaaaaac ctgcgggtgt ttattgaggc ggcaaaggca cgggcggaag
     5701 cgctggacca tgtgttgttt gcggggccac cggggttggg caaaacaacg ctggcacaga
     5761 tcatggcgcg ggagctgggg gtgaatttcc gcgccacgtc cggcccggtg atagccaagg
     5821 cgggtgatct tgcggcactg cttaccaact tggaagacag ggacattctg ttcatcgatg
     5881 aaattcaccg gcttagtcct gctgttgagg aaattcttta tcccgccatg gaggactacc
     5941 agcttgacat catgatcggc gagggcccgg cggcgcgctc ggtgcgcatt gaccttgcca
     6001 agttcacgct ta
//
LOCUS       DAORVN010000021         5909 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_8880_length_5909_cov_2.702426, whole genome shotgun sequence.
ACCESSION   DAORVN010000021 DAORVN010000000
VERSION     DAORVN010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 5909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5909
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8880_length_5909_cov_2.702426"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..802)
                     /locus_tag="PKV23_00785"
     CDS             complement(<1..802)
                     /locus_tag="PKV23_00785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX83994.1"
                     /translation="MNDDSYNQNSVIDGTHRAVLAGVELATPTGRLAAADGLTLLARN
                     PHLICHATFLIDRLAFAAGTSKAALRAAKEQKHLDVAELFAFVCPARMATPTPAGFAR
                     SLALDPLSDELETLQLVAEELLLRLSNRHYPGPREAAEIATYLARSNWPWAKPVMAAL
                     LRANPKLDVGTFATGLNVWDRIDEWEDDGVRPPGRQEGVTPEEATQFLDEVLGKFSEP
                     RPQQKNYAGTATYAFGPRQKKSEINILLAEAGTGLGKTLGYLAPAWLWA"
     gene            846..1691
                     /locus_tag="PKV23_00790"
     CDS             846..1691
                     /locus_tag="PKV23_00790"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009491028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HQX83995.1"
                     /translation="MDQKYINLFDRFTHGGMNRRDFMEKLTLLAGSTTAATALLPLLE
                     NNYARAEIIPEGDPRIATQTLAFAGGSGYFAKPSAEGKYPGVLVIHENRGLNPHTKDT
                     ARRMAAEGFAALAVDYLHDLGGTPEDADKARDMIGTLTPEGINATSVAALAAVKANPA
                     ANGKSGAVGFCWGGGTVGALAVADPGLSAGVVYYGRQPADADVPKITAPLLLQYGGLD
                     ERINAGIPAFEAALKANNKVYELYVYEGANHAFNNDTNAARYNKDVAELAWSRTVEFL
                     KKYVA"
     gene            1772..3418
                     /locus_tag="PKV23_00795"
     CDS             1772..3418
                     /locus_tag="PKV23_00795"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008840683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HQX83996.1"
                     /translation="MASSDRMTDPVLSAAALESRAWPFEEARKLLARIKKSKTPKTEI
                     LFETGYGPSGLPHIGTFGEVARTTMVRRAFETMSDIPTRLLCFSDDMDGMRKIPDNVP
                     DRSFLEPHLHKPLTTVPNPFGGDYESFGHHNNTMLRRFLDTFGFDYEFASATDYYKAG
                     KFDAMLRRAAERYDAIMAVMLPTLGEERQATYSPFLPISPTTGRVLYVPMKNVDAKNG
                     TITFADEDGRDTTLPVTGGHVKLQWKPDFGMRWAALGVDFEMFGKDHQTNAVVYDKIC
                     EILGGLPPEHYVYELFLDDKGQKISKSKGNGLTIDEWLTYADTESLSLYMYNRPREAK
                     KLYFDVIPRAVDEYAQFQNAYGKQELKDRLMNPLWHIHGGNPPPPEEGSVSFSLLLNL
                     VAVANTKDKSVLWAFLKRYAPQVSQATHPRLDSLVGYAIRYFTDFVKPNKKYRAATAD
                     ERKALEDLSAALAELPEGSTPEAIQQKVYDIGRRDPYTTVQKDGSVGVAQTWFNMLYQ
                     VLLGEERGPRFGSFAALFGVQNTRTLIGKALKGELTGSPR"
     gene            complement(3399..4034)
                     /locus_tag="PKV23_00800"
     CDS             complement(3399..4034)
                     /locus_tag="PKV23_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013897283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HQX83997.1"
                     /translation="MFSHKQVWAAIDTIAERYGYSASGLAKKSGLDPTSFNPSKRNGP
                     DGRPRWPTTESIANLLEAAGATVEEFADLLSGRKGQPPRRKHIPLLGLARAGKGGYFD
                     DSGFPVGNGWDEIDVPGVTDNTAYALEITGDSMLPVYREGDTIIVSPGATVRKGDRVV
                     VKTTDGQVMAKVMQRQTAKNVELASFNAAHDTKTLDMKDVDWMARIIWASQ"
     gene            4156..4500
                     /locus_tag="PKV23_00805"
     CDS             4156..4500
                     /locus_tag="PKV23_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015931042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF952 domain-containing protein"
                     /protein_id="HQX83998.1"
                     /translation="MPVLYKIMPRQDWETAQANGIYEGSEVDRRDGFIHLSAARQVRA
                     TARKHFSGQGDLLLVAVTEEALGQSLKWEASRGGELFPHIYGNLPLNAVSEVVPLLLV
                     NGSHQFPDRITS"
     gene            4497..5588
                     /locus_tag="PKV23_00810"
     CDS             4497..5588
                     /locus_tag="PKV23_00810"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HQX83999.1"
                     /translation="MSLLNFGFRVLRPVLHCMDAEAAHGLTIKVLKTGLGGKATISAP
                     ANLAISCFGLSFLNPLGLAAGFDKNAEVPDAMLALGFGFVEVGTVTPRPQEGNARPRL
                     FRLGEDRAVINRLGFNNEGHAAVLERLHRRKHKPGILGVNIGANKDSTDRVADYVAGI
                     NAFSEVASYFTINISSPNTPGLRNLQSAAELPALLTRLNQARQTQTRQVPMLLKIAPD
                     LEADEMQAIAECCLDAAVDGVIISNTTLSRPELRSLHAKETGGLSGAPLFDLSTQQLA
                     RFYLLTQGKIPLIGVGGIANADQAWSKICAGASLLQLYSALVFHGPQLVQEILAGLSR
                     KLAVKNFVTLSQAVGTSAARLAHQSPPGR"
     gene            complement(5564..>5909)
                     /locus_tag="PKV23_00815"
     CDS             complement(5564..>5909)
                     /locus_tag="PKV23_00815"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HQX84000.1"
                     /translation="SHGAWLEKYYSRSTLTGFPGEAEMYEALRTGALDVSFGDGLRLI
                     YWLAGSSSRGCCKPLDGAFVDRQFFSNNLSFLTRREDRDTVMAFDYALDRLQEKKISA
                     EIFARYLPGGLW"
BASE COUNT         1194 a         1865 c         1675 g         1175 t
ORIGIN      
        1 tggcccagag ccaggcgggc gccagatagc ccagggtttt gccaagtccg gttcccgctt
       61 ccgccagcag gatattgatt tcgcttttct tctgccgggg cccaaaggca taggttgccg
      121 tgcccgcata atttttctgc tgcggccgcg gctcggagaa tttccccagc acttcatcca
      181 gaaactgtgt ggcttcttcc ggcgtcaccc cttcctgccg ccccggtggc cgcacgccat
      241 cgtcttccca ctcgtcgatc cggtcccaca cattgaggcc ggtggcgaaa gtgccgacat
      301 caagcttggg gttggccctg agcaacgctg ccatcaccgg ttttgcccac ggccagttgc
      361 tccgcgccag ataggtggca atttcggccg cttcgcgcgg gcccggataa tggcggttgc
      421 tgaggcgcag caaaagctcc tcggccacaa gctgcaaggt ctcaagctcg tcggacaggg
      481 gatcaagcgc cagcgaccgg gcaaatccgg caggcgtcgg cgtcgccatg cgcgccgggc
      541 agacgaaagc gaaaagctcc gccacatcca aatgcttttg ttccttggcg gcccgcagcg
      601 cagccttgga agttccggcc gcaaaggcca gccggtcaat caggaaggtc gcatggcaga
      661 tcagatgcgg atttcgcgcg agcagggtga ggccatcggc cgccgccaat cgccccgtgg
      721 gtgttgccag ttcaacgcct gcaagcaccg ccctgtgggt gccgtcgatc acgctatttt
      781 gattatagga atcatcattc atccctagtc tttaagcagg ttacgcggag aaaaggagcc
      841 ccgtcatgga ccagaaatac atcaaccttt ttgatcgctt cacccatggc ggcatgaacc
      901 gccgcgactt catggaaaag ctcactttgc ttgcgggcag caccacggcg gccaccgccc
      961 tgctgccgct tctggaaaac aattatgccc gcgccgaaat cattcccgag ggcgacccgc
     1021 gcattgcaac ccagacgctc gcgtttgccg gcggcagcgg ctatttcgcc aaacccagcg
     1081 ccgaaggcaa atatcccggc gttctggtca ttcatgaaaa ccgcggcctc aatccgcaca
     1141 ccaaggatac agcccggcgc atggcggcgg aaggcttcgc cgccctcgcc gttgattatt
     1201 tgcatgacct gggcggcacc cccgaggatg ccgacaaggc ccgcgacatg atcggcacgt
     1261 tgacgccgga aggcatcaat gccacttcgg tggcggccct tgccgcggtg aaagccaatc
     1321 cagccgccaa tggcaagtca ggtgccgtgg gcttttgctg gggcggcggc acagtcggcg
     1381 ccctggccgt ggccgatccg ggcttgagcg ccggggtggt ttactatggc cgccagcccg
     1441 ccgatgcgga cgtgcccaag ataacagctc ccctgcttct gcagtatggt ggccttgatg
     1501 agcgcatcaa cgctggcatc cccgcctttg aagcggccct caaggccaac aacaaagtct
     1561 acgaactcta tgtctatgag ggcgccaacc acgccttcaa caatgacacc aatgcggcgc
     1621 gctacaacaa ggatgtcgct gaactggcgt ggagcagaac ggtggagttt ctcaagaaat
     1681 acgtggcttg agggcactta attgcgccaa agccggcagt gcgattgacg aagccttgag
     1741 acatgcctaa caacgccggg catttgtctg aatagcctcg agtgatcgca tgactgaccc
     1801 cgtcctttcc gctgccgccc tggaaagccg cgcctggcct ttcgaggaag cacggaaact
     1861 tctggcgcgg atcaagaaga gcaagacgcc aaagacggaa atcctgtttg aaaccggcta
     1921 cggtccctca ggcctgccgc atataggcac cttcggcgaa gtggcccgca ccaccatggt
     1981 gcgccgcgcc tttgaaacca tgtcggatat tccaacccgg cttctctgct tctccgatga
     2041 catggacggg atgcgaaaga ttcccgacaa tgtcccggac cgcagtttcc ttgagccgca
     2101 tctccacaag ccgctcacta ccgtacccaa tccctttggc ggcgattacg aaagcttcgg
     2161 ccaccataac aacaccatgc tgcgccgctt cctcgatacc ttcggtttcg actatgaatt
     2221 cgccagcgcc acggactatt acaaggcggg caaattcgat gccatgctgc gccgcgccgc
     2281 cgaacgctat gacgccatca tggccgtaat gctgccgacg ctcggcgagg aacgccaggc
     2341 cacctacagc ccctttctgc ccatctcgcc caccaccggc cgcgtgctct acgtgccgat
     2401 gaagaatgtg gatgccaaaa atggcaccat cacttttgcc gatgaggacg gacgcgatac
     2461 gacgttgccg gtcaccggcg gccatgtgaa gctgcagtgg aagccggact tcggcatgcg
     2521 ctgggctgcc cttggcgttg atttcgaaat gttcggcaag gaccaccaga ccaacgccgt
     2581 ggtctatgac aagatctgcg aaatcctcgg cggccttccg cccgagcatt acgtctacga
     2641 actgttcctc gacgacaagg gccagaagat ttccaaatcc aagggcaacg gcctcaccat
     2701 tgatgaatgg ttgacctatg ccgacacgga gtcgctttcg ctctacatgt acaaccgccc
     2761 gcgcgaagcc aagaagcttt acttcgatgt gatcccgcga gccgttgacg aatatgccca
     2821 gttccagaac gcctatggca agcaggagct caaggaccgc ctgatgaatc cgctctggca
     2881 tatccatggc ggaaatccgc cgccgcccga agaaggttcc gtctcgtttt cattgctgct
     2941 caatctcgtg gccgtggcca acaccaaaga caagtccgtg ctttgggcct ttctcaagcg
     3001 ctacgcgccg caggtttcgc aagcgaccca cccgcgcctt gacagcctgg tgggctacgc
     3061 tatccgctac ttcactgatt tcgtgaagcc aaacaagaaa tatcgcgcgg caactgcgga
     3121 tgaacgcaag gcgttggagg atttatccgc cgccctggct gaattgccgg agggctcaac
     3181 cccggaagcc attcagcaga aggtctatga catcggccgg cgcgatccct ataccaccgt
     3241 gcaaaaggat ggttctgtcg gcgtggccca gacctggttc aacatgctct accaggtttt
     3301 gctcggcgag gaacgcggcc cccgcttcgg ctccttcgcc gcattgtttg gtgtgcagaa
     3361 cacccggaca ttgatcggaa aagcgctgaa gggcgagctt actggctcgc ccagataatc
     3421 cgcgccatcc agtccacgtc cttcatatcc agcgtcttcg tgtcatgggc cgcgttgaac
     3481 gaggcaagct cgacattctt ggcggtctgc cgctgcatga ccttggccat cacctggcca
     3541 tcggtggttt tcaccacgac ccggtcgccc ttgcgcaccg tggcgccggg tgaaacgatg
     3601 atggtgtcgc cctcgcggta aaccggcagc atcgaatcgc cggtaatttc gagcgcgtag
     3661 gccgtattgt cggtcacccc cggcacgtcg atttcatccc agccgttacc gaccggaaag
     3721 cccgaatcat cgaagtagcc gcccttgccg gcccttgcca ggccgagcag ggggatgtgc
     3781 ttgcgccgcg gcggctggcc cttgcggccc gagagaagat ccgcgaattc ctcaaccgtg
     3841 gctcctgccg cctccagcag attggcgatg ctttctgtgg tgggccaacg cggcctgccg
     3901 tcaggcccgt tacgcttgga cggattgaac gaggtagggt caaggcccga cttcttggcc
     3961 agtcccgacg ccgaatagcc atacctctcc gcgatcgtgt caatcgcggc ccaaacctgc
     4021 ttgtggctga acatttttgc catatcccgt acctgatatt taatcctcta aactaggaac
     4081 gaatacctca agctcctttg gttgtccaga gccaattgca tggctatggt gcggcgcaaa
     4141 tcagcggggt ctaaggtgcc tgttctctac aaaataatgc cgcggcagga ctgggaaacg
     4201 gcacaggcga atggcattta cgagggatcg gaagtggacc ggcgcgatgg ctttattcat
     4261 ctctcggccg cccgccaggt ccgcgccacg gcgcggaaac atttttccgg acaaggcgat
     4321 ttgcttttgg tggctgtcac ggaggaggcg ctgggccaaa gcctgaaatg ggaagcctcg
     4381 cgcggcggcg aactgtttcc ccatatctac ggcaacttgc cactcaacgc cgtgagcgaa
     4441 gtcgtgccgc tgctgctggt gaacgggtcg caccagtttc ctgacaggat tacttcatga
     4501 gcctgctcaa ttttggcttc agggtgctgc gccccgtgct ccactgcatg gacgcggagg
     4561 cggcccatgg cttgaccatc aaggtgctga agacgggcct tggcggcaaa gccacaatca
     4621 gcgccccggc aaatcttgca atttcgtgtt ttggtctgag ctttctaaat ccgctgggcc
     4681 ttgccgccgg cttcgacaag aatgctgaag tgccggacgc catgctggcc cttggctttg
     4741 gctttgtcga agtgggaacc gtaacgccac gcccgcagga gggaaatgcc cgtccgcgac
     4801 tgttccgcct gggggaagat cgcgccgtga tcaatcgcct gggcttcaac aatgaaggcc
     4861 atgccgctgt tctggaaaga ttgcaccgcc gcaagcacaa accggggatc ctcggcgtca
     4921 acattggggc caacaaggat tcgactgatc gcgttgctga ctatgttgcg gggatcaacg
     4981 ccttttccga agttgcaagc tatttcacca tcaacatctc ctcgcccaac acgccgggcc
     5041 tgcgcaatct gcagtccgcc gcggaattgc cagcactgct gacacgtttg aaccaggcgc
     5101 gccagacgca aacgcgtcag gtgcccatgc tgctcaagat tgcccctgac cttgaagcag
     5161 atgagatgca agccatcgcc gaatgctgtc ttgatgccgc ggtggatggc gtcatcatct
     5221 ccaacaccac actttcgcgg ccggagcttc gttcgcttca tgcgaaggaa accggcgggc
     5281 tttccggtgc gccacttttt gatctttcga cccagcagct ggcgcgtttt tatctgctga
     5341 cccagggaaa aatcccgctg atcggcgtgg gcggcattgc caatgcggat caggcctgga
     5401 gcaagatttg cgccggcgcc tcgcttcttc agctctactc agccctggtt tttcatggcc
     5461 cgcagctggt gcaggaaatc ctggctggcc tctcacgcaa actggcggtg aaaaattttg
     5521 tcacgctcag ccaggctgta ggaacctcag ccgcacgact tgctcaccaa agcccacccg
     5581 gcaggtaacg cgcgaaaatt tccgccgaga ttttcttttc ctgcagccgg tccagggcat
     5641 agtcaaaggc catcaccgtg tcccggtcct cgcgccgggt gaggaaggaa agattgttgc
     5701 tgaaaaactg ccggtccacg aaggcgccgt ccagcggctt gcagcagccg cgcgaagaac
     5761 tgcccgccag ccagtagatc agccgcaggc cgtcaccgaa ggaaacatcc agtgccccgg
     5821 tgcgcaacgc ctcatacatt tccgcttcac cggggaaacc cgtgagggtc gagcgcgaat
     5881 agtatttttc cagccacgcg ccgtggctc
//
LOCUS       DAORVN010000022         5842 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_9016_length_5842_cov_2.856748, whole genome shotgun sequence.
ACCESSION   DAORVN010000022 DAORVN010000000
VERSION     DAORVN010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 5842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5842
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9016_length_5842_cov_2.856748"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..796
                     /locus_tag="PKV23_00820"
     CDS             <1..796
                     /locus_tag="PKV23_00820"
                     /inference="COORDINATES: protein motif:HMM:TIGR01470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HQX84001.1"
                     /translation="EPAVNEAVSAEARGRGIPVNAIDVAEISTFLTPSIVDRDPVVIA
                     IGTEGTAPVLGQGIRARLDAELPQALGELAAKANALRSRVAKEVPHGNRRRSFWAKFF
                     FGRIRDAVVAGDVEGYAHELSIALKDEAAPSLGRISIVGAGPGDPELLTLKAQRKLLE
                     ADVIVHDRNVSSSILELARRDAERFAVSTTQFDISTVLARQAQAGKHVVFLVSGDVDH
                     GSDEVSVLEQHGFAVDIVPGIVGETARPFPFMSRDDIASEMLRAAS"
     gene            793..1131
                     /locus_tag="PKV23_00825"
     CDS             793..1131
                     /locus_tag="PKV23_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008127635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2849 domain-containing protein"
                     /protein_id="HQX84002.1"
                     /translation="MSEVEKGQVMTANRLRDGEVVFLTRSGEWNEKIDEAVLALEPQA
                     TAALEARANEDVKATLVTGSYLFDAERVGGKIRALHMRERIRTLGPTVRLDLGKQAEG
                     TGGAFAFKEA"
     gene            1135..2790
                     /locus_tag="PKV23_00830"
     CDS             1135..2790
                     /locus_tag="PKV23_00830"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008197335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HQX84003.1"
                     /translation="MYRYDEFDHQFVQERTAQFRDQANRRLSGALTEDEFRPLRLMNG
                     LYLQLHAYMLRVAIPYGTLSSKQMRQLAYIADKWDRGYGHFTTRQNIQYNWIKLEDTA
                     DILAALAEVEMHAIQTSGNCIRNVTTDHWAGAAADEIADPRPVGELLRQWSSLHPEFS
                     YLPRKFKIAVTGAPNDRAAVKVHDIGLRLHRNDAGAIGWEVMVGGGQGRTPMIAVTVR
                     DFLPEKELVAYIEAIMRVYNLYGRRDNKFKARIKILVHEIGAEKFKAEVEAEYERRPE
                     QTTHVEEKELQRIAAYFAPPAFEKLSRFSPAFEEAKLLHPGFARWARNNLAAHRVDGY
                     TIVNISLKPEGGIPGDATSDQMRLMAELAERYSHDELRVSHPQNIILPHVKKDDALAI
                     WQALVPAELATANYGLAGDIIACPGMDYCSLATARSIPISQAISRKFADLDVQEKIGP
                     VKINISGCINACGHHHVGHIGILGLDKKGEEFYQITLGGSSAENASIGSILGPGFAGH
                     EVADAIEKILTTYVKLRQPGEEFLYAYRRLGPQPFKEALYAAA"
     gene            2777..3319
                     /locus_tag="PKV23_00835"
     CDS             2777..3319
                     /locus_tag="PKV23_00835"
                     /inference="COORDINATES: protein motif:HMM:NF017849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF934 domain-containing protein"
                     /protein_id="HQX84004.1"
                     /translation="MPLLKNNAFITDIWLHLGAEDALPEGGNVTIPFSRLKNEWESLA
                     KFPGQLGAVLSNTDHAEDVAHFLPHLALVVLPFPAFVDGRAYSIARQIRLLGYRGELR
                     ATGNILPDQFQYMTQVGFSSFEVSERFPLQSWQQASKQMSLAYQRGLYRRVGEAEVWS
                     ERHADTAPEPTPWEEQPHAG"
     gene            3309..4040
                     /locus_tag="PKV23_00840"
     CDS             3309..4040
                     /locus_tag="PKV23_00840"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014284036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HQX84005.1"
                     /translation="MPDSIIDNSVVERYAGLEGRDLIMSILRDYPGKTALVSSFGAES
                     AVLLHMVSEVDTGLPIIFLDTGKLFQETLDYRDKLIVELRLTNVRSVKPEAFDILAHD
                     ATGLLHQSSTDSCCHIRKTVPLEKALAEFDVVISGRKRFHGAARADLDYVSTADDRLK
                     VEPLAGFSALDIQSYMNSHHLPSHPLRLRGYRSIGCEPCTSLGGTDDDPRAGRWAGTD
                     KTECGIHFTANGQIIRTVQRQAVNA"
     gene            4070..4831
                     /locus_tag="PKV23_00845"
     CDS             4070..4831
                     /locus_tag="PKV23_00845"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HQX84006.1"
                     /translation="MHEFWAFVLVGFFAQLVDGALGMGFGVISASVLLAQGVPPALVS
                     ASVNAAKLPTTGTAALSHYLHKNLDWRIIASLCVFGALGGILGATVLTSLKGTVLFIL
                     INVYLLLIGGLIIYRGLRDAVPRVFRLGFTRVIGFAGGLIEGIGGSWGPIVTTSLLGA
                     GTQSRYAIGSSNFSEFVVSIAVFATFVAAFAMGFWDGGSDWRDVALPVAGLVLGGIPA
                     AVVGGYLSKIAPKRPLTVAVGLLAFSIGIYRFATM"
     gene            complement(4835..>5842)
                     /locus_tag="PKV23_00850"
     CDS             complement(4835..>5842)
                     /locus_tag="PKV23_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HQX84007.1"
                     /translation="RYGNPTVSMLEERMRLLEGAEACYAVASGMSAAFGALACQLKVG
                     DHVVASRSLFGSNYQIITAILPKFGITYDLVDGRDASAWKQAMKPQTKCVFFETPSNP
                     LLEVLDIQMICDIAHKAGAVAIVDNVFATPILQKPMQLGADIVFYSSTKHTDGQGRVF
                     GGAILCSKKFKEELLKPFLRHTGPTLSPFNAWVLLKGLETLKLRVEAQSRAAAAVAEA
                     LASTKGVERIYYPHHDTHAQVKLARGQMKMGGTMVSFDVAGGKAKAFDVLRKLSIIDI
                     SNNLGDAKSLITHPATTTHRNIGPEARAMMGITDGNLRLSVGLEDVDDLVGDLRQALK
                     P"
BASE COUNT         1159 a         1669 c         1751 g         1263 t
ORIGIN      
        1 ggagcctgcg gtgaatgagg ccgtcagtgc agaagcacgg gggcgtggca ttcccgtcaa
       61 tgcaattgac gtggccgaga tttcgacttt cctcacccca tcgattgtgg accgtgaccc
      121 ggtggtgatt gccattggaa cggaaggcac ggccccggtt ctgggccagg gcatccgggc
      181 ccggcttgac gcggagctgc cgcaggcgct gggtgaactg gctgccaagg ccaatgcgct
      241 gcgttcgcgg gttgccaagg aggtgccgca tggcaatcgc cggcgttcgt tctgggccaa
      301 gtttttcttc ggccggatcc gcgatgcggt ggtggcaggt gatgtggagg gctatgcaca
      361 cgaactcagc atcgcgctga aagacgaagc ggccccttcg cttggccgga tttcaattgt
      421 gggtgcaggc cccggcgatc cagaactgct gaccctcaag gcgcagcgca aactgcttga
      481 ggccgatgtg atcgttcatg atcgcaatgt ttcctccagc attctggaac tggcacggcg
      541 cgacgcggaa cgatttgccg tgagcaccac acagtttgac atcagcaccg tgctagcgcg
      601 gcaagcccag gcgggcaagc atgtggtatt cctggtttca ggcgatgttg atcacggttc
      661 tgacgaagta tcggttctgg agcaacacgg ctttgcggtt gatatcgttc cgggcattgt
      721 aggcgaaaca gcaaggccat tccccttcat gtcgcgggac gatatcgcct ctgaaatgct
      781 gagggcggca tcatgagcga ggttgaaaaa ggccaggtga tgaccgccaa ccggctgcgc
      841 gacggggaag ttgtattcct aacgcgctca ggcgagtgga atgaaaaaat cgacgaggct
      901 gtgctggcgc tggaaccaca agccactgcg gcacttgagg cgcgggccaa tgaagatgtg
      961 aaagccacgc tggtcactgg ctcctatctg tttgatgcgg agcgcgtggg cggaaaaata
     1021 cgggcgcttc atatgcgcga gcgcatccgt acccttggcc ccacggtgag gcttgacctc
     1081 ggcaagcagg ccgagggcac gggcggcgca ttcgccttca aggaggcttg atccatgtac
     1141 cgctacgatg aattcgacca tcaattcgta caggaacgca ccgcgcaatt ccgcgaccag
     1201 gccaaccgcc gcctgagcgg cgcgctgacc gaggacgaat tccgcccgct gcgcctcatg
     1261 aacgggcttt atctgcagct tcatgcctat atgcttcggg tggcgatccc ctacggcacc
     1321 ctgtcttcca agcagatgcg gcagctcgcc tatatcgccg acaagtggga ccgcggctat
     1381 ggccatttca ccacacggca gaacattcaa tacaactgga tcaagcttga ggacacggcg
     1441 gacattctgg cggccctcgc cgaagttgaa atgcacgcaa tccaaacctc gggcaattgc
     1501 atccgcaacg tcacgacgga tcattgggcg ggtgctgcgg cagacgagat cgccgatccg
     1561 cggcccgtgg gcgaattgct ccggcaatgg tcgtcgctgc atccggaatt ttcctatctg
     1621 ccgcgcaagt tcaagatcgc ggtcacgggt gcgcccaatg accgtgcagc agttaaggtt
     1681 catgacatcg gattgcggct gcacaggaat gacgcgggtg ccattggctg ggaagtcatg
     1741 gtgggcggtg gccagggccg cacgccaatg atcgcggtca cagtgcgcga tttcctgccg
     1801 gaaaaggaac tcgtggctta tatcgaagcg atcatgcggg tctataatct ctatggccgg
     1861 cgcgacaaca aattcaaggc gcgcatcaag attcttgtgc acgaaatcgg tgcggagaag
     1921 ttcaaagcgg aggttgaagc ggaatatgag cgccgtcctg agcagaccac tcatgtcgag
     1981 gaaaaggaac tgcagcggat tgcggcttac tttgcccccc cagcatttga aaagctgtca
     2041 cgcttttccc cggcgttcga ggaagccaag ctcttgcatc ccgggtttgc ccgttgggcc
     2101 cgcaacaatc tcgcggcgca ccgggttgat ggctacacca tcgtcaatat ttccctcaag
     2161 ccagaaggcg gcattccggg cgatgcgacc tctgaccaga tgaggctgat ggcggagttg
     2221 gccgagcgct attcgcatga cgaactgcgg gtaagccatc cgcagaacat cattcttcct
     2281 cacgtgaaga aggacgatgc actggccata tggcaagctc ttgtgcccgc tgaactggcc
     2341 acggcgaact atggcctcgc aggtgacatc attgcctgcc ccggcatgga ttattgctcg
     2401 ctggcgacgg cccgttccat cccgatcagc caggcgattt cacggaaatt tgccgacctg
     2461 gacgtgcagg aaaaaatcgg cccggtgaag atcaacatct cgggctgcat caatgcctgc
     2521 ggacaccatc atgtgggcca catcggcatt ctcgggctgg acaagaaggg cgaggaattt
     2581 taccagatca cgcttggcgg ctcatctgcc gagaacgctt cgattggctc catcctcgga
     2641 ccgggctttg ccggacatga agtggccgat gccatcgaga agattctgac gacttacgtg
     2701 aagctgcgcc agccgggcga ggaattcctt tatgcctatc gccgcctggg gccgcaacct
     2761 ttcaaggagg ctttgtatgc cgctgcttaa gaacaacgcc ttcatcacag acatctggct
     2821 gcatcttggc gccgaagatg ccttgccgga gggcggcaat gttaccattc ctttttcgcg
     2881 cctcaagaat gagtgggaga gcctagcgaa gttcccgggt caactcggcg ctgttttgag
     2941 caacacggat catgcggaag acgtggcaca tttcctgccg catctcgccc ttgttgtttt
     3001 gccatttccc gcctttgtcg atggtcgcgc ctattccatc gcccggcaaa tccgcctgct
     3061 gggataccgc ggcgaattgc gcgccacggg caatatcttg cctgaccagt ttcagtatat
     3121 gacccaagta gggttcagca gttttgaagt ttccgagcgc tttccgcttc aatcctggca
     3181 gcaggcttca aaacaaatgt cgcttgccta tcaacgcggg ctttaccgcc gggtcggcga
     3241 agcggaggtg tggagcgaac gtcatgctga cacagcgccg gagcccacgc catgggagga
     3301 acagccccat gccggatagt atcatcgaca acagtgtagt cgaacggtac gccggtcttg
     3361 aaggccggga cctgatcatg tccatcctgc gcgactatcc cgggaagacg gccctggttt
     3421 cttctttcgg cgccgagtct gccgtgctcc tgcacatggt ttcggaagtg gacactggct
     3481 tgccgatcat ttttctcgat acgggcaaac tttttcagga gacgctggat taccgcgaca
     3541 agctgattgt ggagcttagg ctcacgaatg tgcgctcggt caagccggag gcttttgata
     3601 ttctcgctca tgatgctacg ggcctgttgc accagagcag cacggactcc tgctgccata
     3661 tccgcaagac tgtaccgctc gaaaaggcgc tggcggaatt tgatgtggtt atttccggcc
     3721 gcaagcgatt ccatggcgcg gcccgggctg acctcgacta tgtcagcacc gccgatgacc
     3781 gtttgaaggt tgaaccgctt gcgggatttt cggcgctcga tatccagtcc tatatgaaca
     3841 gccatcatct gccctcgcat cccctcaggc tcaggggcta ccgctccatc ggctgcgaac
     3901 cctgcacctc gcttggcggc actgatgatg atccgcgcgc cggacgctgg gctggaaccg
     3961 acaagacgga atgcggcata catttcaccg ccaacggaca aatcatccgc acggttcagc
     4021 gccaggctgt gaacgcttga tcaaacactg gttttagggg aaccacccga tgcatgaatt
     4081 ctgggctttt gtgcttgtgg gatttttcgc ccagcttgtt gacggtgccc tgggtatggg
     4141 atttggcgtt atctcggcgt cagtgcttct ggcgcagggt gtgccccctg ccctcgtttc
     4201 ggcaagtgtg aacgccgcca aacttccaac gacgggaaca gcggcgctct cgcactacct
     4261 gcacaaaaat ctcgactggc gcattatcgc ctcgctgtgc gttttcggtg cccttggggg
     4321 catccttggt gccacagtgc ttaccagtct caagggtacg gtcctgttta tcctgatcaa
     4381 cgtctatctg ctgctcattg gcggcctgat catctatcgc ggcttgcggg atgcagttcc
     4441 gcgtgtcttc cgcttggggt ttacccgggt gattggtttt gccggggggc tcattgaggg
     4501 catcggcgga agctggggcc ctatcgtgac caccagcctc ctaggtgcgg gtacccagtc
     4561 ccgctatgcc attggctcca gcaatttctc ggaatttgtc gtgagcattg ccgtattcgc
     4621 aacttttgtc gcggcgtttg ccatgggatt ctgggacggc ggctcggatt ggcgtgatgt
     4681 cgcactgcct gttgcgggct tggtccttgg cggcattccc gctgcggttg tgggcggtta
     4741 tctttccaag atcgcgccca aacgaccgct cactgttgcg gtagggctgc tggccttcag
     4801 catcggcata taccgcttcg caacgatgta gtcttcaggg cttcagagcc tgcctgagat
     4861 cgccaacaag atcatcaaca tcttccaggc ctaccgacag acggaggttg ccatcggtaa
     4921 tgcccatcat ggcgcgggcc tcgggtccga tgttgcggtg ggtcgtcgtg gccggatggg
     4981 tgatgaggga cttcgcgtca ccgagattgt tggagatatc gatgatgctg agcttgcgca
     5041 gaacatcgaa ggctttggcc ttgccgcccg ccacatcgaa ggacaccatg gtgccgccca
     5101 ttttcatctg accgcgagcc aactttacct gagcatgggt atcgtgatgc gggtaataaa
     5161 tgcgttcaac gccctttgtt gaagcgagag cttcggcaac ggctgccgcg gcccggctct
     5221 gggcctcaac acgcagtttc aacgtttcca ggcccttgag cagcacccag gcgttgaagg
     5281 gcgaaagtgt cggaccagta tggcggagga acggcttcag cagctcctcc ttgaatttct
     5341 tgctgcagag gatggcgccg ccaaaaaccc ggccctggcc atcggtgtgc ttcgtactgg
     5401 aataaaatac gatatcagcg cccagctgca tcggtttctg caaaatgggc gtggcaaaga
     5461 cattgtcgac gatggcgact gcaccagcct tgtgggcgat gtcgcagatc atctgtatat
     5521 cgagcacctc cagcagcggg ttggacggcg tttcgaaaaa cacgcatttg gtttgcggct
     5581 tcatggcctg tttccaggca ctcgcatccc tgccgtcgac aaggtcatag gttataccga
     5641 acttgggcag aatggcggta atgatctggt aattggagcc aaagagcgag cgcgaagcaa
     5701 cgacatggtc gccgaccttc aactggcatg ccagcgcgcc aaaagccgcc gacatgcctg
     5761 atgccaccgc ataacaggct tcagcccctt ccagcaggcg catgcgttct tccagcatgg
     5821 agacggtggg gttgccgtag cg
//
LOCUS       DAORVN010000023         5822 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_9079_length_5822_cov_2.951795, whole genome shotgun sequence.
ACCESSION   DAORVN010000023 DAORVN010000000
VERSION     DAORVN010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 5822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5822
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9079_length_5822_cov_2.951795"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..292
                     /locus_tag="PKV23_00855"
     CDS             <1..292
                     /locus_tag="PKV23_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015339933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin molybdenumtransferase MoeA"
                     /protein_id="HQX84008.1"
                     /translation="RLFLKPLLNCLQGLPVDEVPTMARLGIAMPANDSRQDHVRAMLE
                     IAADGSRTATPCGKQDSSMQRTSRDAHCLIVRPPNAPAALMGDLVPILNLDF"
     gene            391..1095
                     /gene="lexA"
                     /locus_tag="PKV23_00860"
     CDS             391..1095
                     /gene="lexA"
                     /locus_tag="PKV23_00860"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010659992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HQX84009.1"
                     /translation="MLTRKQLELLLFINERLKEEGVPPSFEEMKDALNLQSKSGVHRL
                     IVALEERGFLKRMANRARALEVIKLPESHSPSLTSKKSGFSPNVIQGSLGKVKSMPAA
                     NDGIPRAVMVPLMGKIAAGVPIAAIQDHTNNISIPPEMLGGGEHFALEVKGDSMIEAG
                     IFDGDTVLIRKGDTANNGEIIVALIDDEEATLKRLRKKGESVALEAANPAYETRIFGP
                     DRVRVQGRLVGLLRKY"
     gene            1178..1792
                     /locus_tag="PKV23_00865"
     CDS             1178..1792
                     /locus_tag="PKV23_00865"
                     /inference="COORDINATES: protein motif:HMM:NF024809.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HQX84010.1"
                     /translation="MKIYGDTISPFVRMCLVTAHECGLSGKLEHVREAVKPTQANPKL
                     TALSALGKIPILETDHHHPLYDSRVIIEYLAHVAGNKTLIPDDGVKRFRILTLQALSQ
                     GLADVCVAYRYETAARPLGLQWADWMARTEQRINAAMDDMDRNWQAELTDVTAGTIAM
                     AVALAYIDFRLPAFKWRDTRPNLSAFHEKFSARESMLKTALSAS"
     gene            1792..2970
                     /locus_tag="PKV23_00870"
     CDS             1792..2970
                     /locus_tag="PKV23_00870"
                     /inference="COORDINATES: protein motif:HMM:TIGR01988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HQX84011.1"
                     /translation="MADCKVIVAGAGPAGLAAAALLAKEGLATTVVAPAAGEDPRTVA
                     LMQPSIQLLRYLDIWPGKLESETSPLRKLRIIDDTGSLLVAPNLEFASQDLNLEAFGW
                     NVPLALLLPVLRRRAEDLGVTFVEATATGAVSRDGAIHVTLSNSTEISAALCLAADGA
                     NSVLRKAAGIATDTWSYDQTAIATSFAHSMGHDNISTEYHKPAGPFTTVPLPENRSSL
                     VWMERPARAEALMVMDDKQLAAEIQIETHGELGLISNLGPRKSFPMKGLTARQFAKDR
                     VILIGEAAHVVPPIGAQGLNMSLRDAALAADLILAAKDPGSAGVLAEYNAVRRAEVVP
                     RQHLIDLMNKSLLLGFLPLEGARALGLTAVHYFAPLKRFIMERGLQQTDNLPFAMRAS
                     "
     gene            3013..3351
                     /gene="hspQ"
                     /locus_tag="PKV23_00875"
     CDS             3013..3351
                     /gene="hspQ"
                     /locus_tag="PKV23_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009760244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HQX84012.1"
                     /translation="MSEMPTAKFQIGQIVKHRLFSFRGVIFDVDPVFSNTEEWWSSIP
                     EESRPRRDQPFYHLLAENDDTEYVAYVSEQNLLVDVTGTPVRHPQVNQYFKGFDAKKN
                     SYLLREHRAH"
     gene            3362..4582
                     /locus_tag="PKV23_00880"
     CDS             3362..4582
                     /locus_tag="PKV23_00880"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TCR/Tet family MFS transporter"
                     /protein_id="HQX84013.1"
                     /translation="MTAHTPSRHAVTFIFITVFLDMVGFGLIMPVLPRLIEDVSGADL
                     AGASLLAGWLFFAYGGMQFLCGPLLGNLSDAYGRRPILLLSVFGLGVDYLLTAFAPNM
                     FWLFVGRTLAGLCGASYTTANAYLADITEPENRAKVFGYMGAAFGLGFIIGPAIGGIL
                     GEYGPRVPFFAAACISILNVAYGYLVLPETLPRESRRPFSLARSNPIGVFKVFATYRQ
                     ALPLCIVMAMYFFATSVYPAIWAFWGIARFNWSEMTIGWTLSVFGLITAVVQGGLTGP
                     AVKWLGERNTTILALATSVIAAAGYGVATGMSMVLILFLVHAPEGFAQPALTALMSKD
                     APPDAQGELQGGIASAQNLALLVGTVMFAQIFGYFLQDGSPIQSPNVAYFISAALGLM
                     ALSVFIHVTRRRSA"
     gene            complement(4583..4753)
                     /locus_tag="PKV23_00885"
     CDS             complement(4583..4753)
                     /locus_tag="PKV23_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006203375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX84014.1"
                     /translation="MRLSAPTGLVFVVALIVAVVAVLEAMGTISFVPLASVWIMAIAY
                     AVLAVGCLIRGF"
     gene            complement(4833..>5822)
                     /gene="glmS"
                     /locus_tag="PKV23_00890"
     CDS             complement(4833..>5822)
                     /gene="glmS"
                     /locus_tag="PKV23_00890"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018391161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HQX84015.1"
                     /translation="TVKMPEKLPFEFTSLDRLTITACGTASYAGLVAKYWFERIARLP
                     TEVDIASEFRYREAPLTPNGLSIVISQSGETADTLASLRYSKSQGQHTLALVNVPEST
                     IAREADVALRTFAGPEIGVASTKAFTCQLAALACLALLAAKQRGQLSKALEQELVGAL
                     VEAPRHMSEILKQEKHIAGVAREIAHAKDVLYLGRGINYPIAMEGALKLKEISYIHAE
                     GYAAGELKHGPIALVDENMPVIVIAPRDELYDKTISNMEEVAARGGKIVLLTDDESGN
                     HRTFHRIRVPKAHSFISPLLYAIPVQLLAYHAAVFIGTDVDQPRNLAKSVTVE"
BASE COUNT         1192 a         1805 c         1558 g         1267 t
ORIGIN      
        1 ccgactcttt ctgaaacccc tgctcaattg cctgcaaggc ctgccagttg acgaagtgcc
       61 caccatggca cgtttgggca ttgcaatgcc agccaatgac agccgccagg accatgtgcg
      121 ggcaatgctg gaaattgccg ccgatggctc ccgcaccgcc actccttgtg gcaagcagga
      181 ctcatccatg cagcgcactt cccgcgatgc ccactgcctc attgtccgcc cgccaaacgc
      241 cccggcagcc ctcatgggcg acctcgtccc gatcctcaat ctcgacttct agccgccaca
      301 aaaagtaaac agttgcagaa ctaaaccgga acctgtatag atgttgcagg tttgttccga
      361 ttccgtctct agcaatgaaa gcgatctccc atgctcaccc gcaaacaact cgaactgctg
      421 cttttcatca atgagcgcct gaaggaggaa ggggttccgc cgtccttcga ggaaatgaag
      481 gatgcactga acttgcagtc caaatccggc gttcatcgtc tgatcgtggc cctggaagag
      541 cgcggctttc tcaagcgcat ggccaaccgc gcccgcgccc tggaagtaat caagctgccg
      601 gagtcccatt ccccgagcct gacgtccaaa aaatccggtt tctcgcccaa cgtcattcag
      661 ggaagcctcg gcaaggtgaa atccatgcct gccgccaatg atggaattcc ccgcgcggtg
      721 atggtgcccc tcatgggcaa gatcgcggcg ggtgtgccga ttgccgccat ccaggatcac
      781 accaacaata tttccatccc tcctgaaatg ctcggcggcg gtgagcactt tgccctggag
      841 gtcaaaggcg actcgatgat cgaagccggc atattcgatg gcgatacggt cctcatccgc
      901 aagggcgata cggccaataa tggcgaaatc atcgtggccc tcatcgatga cgaggaagca
      961 accctcaagc gcctgcgcaa aaagggtgaa tccgtagccc tcgaagcggc aaacccggcc
     1021 tacgaaaccc gcattttcgg gccggaccgg gtgcgtgtac agggccgcct tgtcggcctg
     1081 ttaagaaaat actgaggcgg cgtttcgccc ttcattgggc gccgcccatc gtctaagctg
     1141 gggttgcaga ctgaaccagt tcggaaggta agcccctgtg aagatttatg gcgatacgat
     1201 ttctcccttt gtacgcatgt gcctcgtcac tgcccatgaa tgcgggcttt cgggcaagct
     1261 cgagcatgtc agggaagccg taaagcccac ccaggcaaat ccaaagctca cggcgctctc
     1321 tgcccttggc aaaatcccca ttctcgaaac cgaccaccat cacccgctct atgacagccg
     1381 tgtcatcatt gagtacctgg cccatgtcgc aggcaacaaa accctgattc ccgacgacgg
     1441 cgtcaagcgc ttccgcattc tcacccttca ggccctcagc cagggcctgg ctgatgtctg
     1501 tgtggcctat cgctatgaaa cggctgcgcg cccgctagga ttgcagtggg cggactggat
     1561 ggcccgcacc gaacaacgca tcaatgccgc catggatgat atggacagga actggcaggc
     1621 tgaacttacc gatgtcaccg ccggaaccat cgcgatggcg gttgctctcg cttacatcga
     1681 tttccgcctt cccgccttca agtggcgcga cacgcgcccg aacctcagcg cctttcatga
     1741 aaaattttcc gcccgcgaaa gcatgctgaa aaccgctctg tcggcaagct gatggcagat
     1801 tgcaaagtca tcgttgcagg cgcgggcccc gctggcctag cggcggcagc cctgcttgcc
     1861 aaggaaggtt tggcaaccac agtcgttgcc ccggcagcag gtgaagaccc tcgcaccgtc
     1921 gccctgatgc aaccctccat ccagcttctg cgctaccttg atatctggcc agggaaactc
     1981 gaaagcgaga cgtccccatt gcgaaagctc cggatcattg atgatacggg atcgctcctg
     2041 gtcgcaccga accttgagtt tgcatcacag gacctgaacc tggaggcctt tggctggaac
     2101 gttccgcttg ccctgcttct ccccgtcctc cgccgacgcg ctgaagatct tggggttacg
     2161 tttgtcgaag ccaccgcgac cggagccgtt tcgagggacg gtgccattca tgtcaccttg
     2221 tccaattcga ccgaaatttc tgccgccctt tgccttgctg cagatggcgc aaattcagtc
     2281 ctgcgcaaag ccgcaggcat tgccactgat acctggtcct atgaccaaac tgcgatcgca
     2341 acaagcttcg ctcattccat gggccatgac aacatctcga cggaatatca caagcccgcc
     2401 ggccctttca ctacggtgcc tttgcccgaa aaccgttcaa gcctggtgtg gatggaacgt
     2461 cccgcccgcg ccgaagccct catggtcatg gacgataagc aactggccgc cgaaatacaa
     2521 attgagaccc atggcgaact ggggctcata tcaaaccttg ggccccgcaa atcattcccc
     2581 atgaaaggac tcaccgcccg gcagttcgca aaggatcgcg tgatattgat cggcgaggcc
     2641 gcccatgtcg tgccgcccat tggtgcgcag ggactgaaca tgtccttgcg tgatgcggca
     2701 ctcgccgctg atctcattct cgccgccaag gatcccggaa gtgccggcgt cttggccgag
     2761 tataatgccg tgcgccgcgc tgaagttgtc ccacgccagc atctcattga tctcatgaac
     2821 aaatccctgc tcttgggatt tctcccgctt gaaggggcac gcgccctggg tcttaccgca
     2881 gtgcactatt ttgccccgct gaaacgcttc attatggaac gtggcctgca gcaaaccgac
     2941 aatctgccct ttgccatgcg ggcttcctaa gagcctcttg ccttccaggc ggactcttgg
     3001 caatcaggtg tcatgagcga gatgccaaca gccaaatttc agatcggcca gatcgtaaag
     3061 caccggctgt tttcgttccg cggcgtgatt tttgatgtgg atccggtttt cagcaacacc
     3121 gaggaatggt ggagttccat ccccgaggag tcacggcccc gccgtgacca gcccttctat
     3181 catctgcttg ccgaaaacga tgacacggaa tatgtggcct acgtctccga gcagaacctt
     3241 ctggtcgacg tgaccggcac gccagtgcgc catccgcagg tcaaccaata cttcaagggg
     3301 tttgacgcaa agaagaatag ttatctgctc cgcgaacacc gcgcgcatta aagctgttcc
     3361 catgactgcg cataccccca gccggcacgc cgtgaccttc atctttatca ctgtcttcct
     3421 cgacatggtg ggcttcggcc tcatcatgcc agtattgccg aggctgatcg aagacgtgag
     3481 cggcgctgat ctcgcgggcg cctcgctgct agcgggctgg ctcttttttg cctatggcgg
     3541 aatgcaattt ctctgcggcc ccctgcttgg aaatctctct gatgcctatg gccgccggcc
     3601 aatcctcctt ctttcggtct ttggccttgg tgttgattac ctgctgaccg cctttgcccc
     3661 caacatgttc tggctcttcg tgggccgcac gctggctggc ctttgcggcg cgtcctacac
     3721 cacggcgaac gcctatctgg ctgacattac ggagccggaa aaccgcgcca aggtcttcgg
     3781 ctacatggga gcggccttcg gcctgggctt tatcatcggc ccggcaattg gcggcatcct
     3841 cggcgaatat ggtccgaggg tccccttttt cgcggcggca tgcatttcaa tcctcaatgt
     3901 tgcttacggc tatcttgtat tgcctgaaac acttccccgg gaatcgcgcc ggccattcag
     3961 cctggcccga tccaatccga taggcgtttt caaggtcttt gcaacctaca ggcaggcact
     4021 ccccctgtgt attgtcatgg ccatgtattt tttcgccact tcggtttatc cggcgatctg
     4081 ggcgttctgg ggcattgccc gcttcaactg gagcgagatg acaattggtt ggactctatc
     4141 agtatttggc ctgattaccg ccgtggttca gggcggcttg actggaccag ctgtaaaatg
     4201 gcttggcgaa cggaatacga ctattctcgc ccttgccaca tcagtcatcg ccgccgcagg
     4261 ctatggcgtg gcaacgggca tgagcatggt attgattctg tttcttgttc atgcgccgga
     4321 aggctttgcc caaccggcct tgacggccct catgtccaaa gatgccccac ccgatgccca
     4381 gggcgaactg cagggcggga ttgccagtgc gcagaatctc gccctgcttg tgggaacagt
     4441 tatgttcgcg caaatattcg gctacttcct gcaggacgga tcaccaattc agtcgcccaa
     4501 tgtggcttac ttcatctccg ccgcactggg cctgatggct ctttcagtat tcatacacgt
     4561 gacacgtcgc aggtcagcgt aatcagaaac ctctgataag gcagccaacg gcaagcaccg
     4621 cataggcaat ggccatgatc cagactgacg cgaggggaac gaacgagatt gttcccatcg
     4681 cctccagcac cgcaacaacc gcgacaatga gcgcgacgac aaagacgagt ccggtagggg
     4741 cgctaagtct catttttcat tctcctcggt tgattcgaat gccgattata gcggaattcg
     4801 ctcacgtctc aaaaataacg gcgcaccagc ccctattcca ccgtcactga tttcgccagg
     4861 ttgcgcggct ggtccacatc cgtgccgata aagaccgccg catgatacgc cagcagttgc
     4921 accggaatcg cataaagcag cggactgatg aaggaatggg ccttgggcac gcggatgcgg
     4981 tggaaggttc tgtgattgcc gctttcatca tctgtaagaa ggacaatttt tccgccgcgc
     5041 gccgccactt cctccatgtt tgaaatggtc ttgtcgtaga gttcgtcgcg cggcgcaatc
     5101 acgatcactg gcatgttctc gtcaaccagc gcaatgggcc cgtgtttgag ttcacctgcc
     5161 gcatagcctt cggcatggat gtaggaaatt tccttgagct tcagtgcacc ttccatggca
     5221 attggatagt tgatgccgcg ccccagataa aggacatcct tggcatgggc aatttcccgc
     5281 gccacgccgg caatgtgctt ctcctgcttc aaaatctccg acatgtggcg gggcgcttca
     5341 accagagccc cgaccagctc ctgctccagc gcctttgaaa gttgcccgcg ctgcttggcc
     5401 gcgagcaatg caaggcaggc aagggccgca agctggcagg taaaagcctt ggttgaagcc
     5461 acgccgattt ccgggcctgc aaaagtgcgc agcgccacat cggcttcgcg cgctatcgtt
     5521 gactccggga cattcaccag cgccagggta tgctgcccct gtgatttgct gtagcgcagc
     5581 gaggccaggg tgtccgctgt ctcgcccgat tgtgaaatca caatagacag tccatttggc
     5641 gtcaggggtg cttcgcggta gcggaattcg gaagcgatat cgacttccgt cgggagccgg
     5701 gcaatgcgct cgaaccagta tttcgccacc agccctgcat aggaagccgt gccgcaggct
     5761 gtgatcgtga ggcggtccag cgaggtgaat tcaaacggca gcttttccgg catcttcacc
     5821 gt
//
LOCUS       DAORVN010000024         5791 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_9188_length_5791_cov_3.419107, whole genome shotgun sequence.
ACCESSION   DAORVN010000024 DAORVN010000000
VERSION     DAORVN010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 5791)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5791)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5791
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9188_length_5791_cov_3.419107"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..942
                     /locus_tag="PKV23_00895"
     CDS             <1..942
                     /locus_tag="PKV23_00895"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HQX84016.1"
                     /translation="RFVGLQNFYKQFFGNDQVHFLGKTDSMGIFGTVFFVVVAAALLW
                     WLYRSLKITSWVGMLGRIISAVMAIFIAWVVAASLVSGNTIGTLLTTLFYVFAGCALQ
                     FVIGTGLAFLCSQPILGKNFFRVIFFIPLMVTPLGVGYAMKMVADVTKGPFEPLLRGF
                     GIEDWVWSADPWAARFVMMICDSWQWIPFIFICMLAALENVPRDHVEAAEVDGASSIQ
                     IFREVTWPQIVPVAVTVLLIRMIEAFKIVDLPNIMTGGGPGSSTESMTLHSLYIWRSN
                     DIGTSAAVAYLLLILTVVVCASLFNYVVLGQLRKARA"
     gene            939..2123
                     /locus_tag="PKV23_00900"
     CDS             939..2123
                     /locus_tag="PKV23_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007790799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HQX84017.1"
                     /translation="MSEASFKLEKANRSLMERLFEPPAVGKMSPVANLVAYLLLFVWS
                     IFVLFPLYWVVITSFKDAAAVNQGPYYIPFVDFQPNLNAWRAQFNTDPFCDMPSILRQ
                     VGLLFHNSFAFLVSPVMTIQPMEPQICKIYLAFTNSIVVSVAATIVCVTIGSMAAYAL
                     ARVQYKPKFGNIMMFVALTVLVIAATSYAGVPWWASTAVALALFFFLARAFGKYFTRT
                     LANGDILFWIISQRILPPIVVIIPIYVMFQAVGLLDTHLALITVYAVANLPIVVWLMY
                     DFFSSLPIELEESAQLDGATRFGIFWEIVLPLTRPGLAATTLLTLILSWNEYLFAVFL
                     STSKVQTMPIMVQAKNAGEKGVFWWEMCAIIVVMIVPVIIMAIILQRFIARGVLLGAV
                     KG"
     gene            2136..3206
                     /locus_tag="PKV23_00905"
     CDS             2136..3206
                     /locus_tag="PKV23_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006204280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQX84018.1"
                     /translation="MASGKTGVPVSFDRIHKSFGPVRVLEELNLQVEPGEFLVLLGAS
                     GSGKTTALRILSGLETATAGRVLIGNRDVTDILPKYRDISMVFQSYALYPHKTVAENI
                     GFPLKVRKVPQAEQDVQIRDAAAQVQLGELLHRYPRELSGGQKQRVALARAIIRRPSV
                     FLMDEPLSNLDAKLRGHMRAELKHMQESFGITTIYVTHDQIEAMTLARRVAILERGVL
                     QQLATPAEIYNNPANLFVAQFIGSPPMNVVQGALEGKNFLNNGAKIATPVSGRIAKAV
                     LGVRPEDCSVVAPEKGGIKGEIYATELIGDHTLVTLKAGSDLLTVKAAKDFTARLGEK
                     AGVAFSSDRLFVFDESNGLRVR"
     gene            3415..5016
                     /locus_tag="PKV23_00910"
     CDS             3415..5016
                     /locus_tag="PKV23_00910"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012482112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase"
                     /protein_id="HQX84019.1"
                     /translation="MQIDSTTRSQLLSLGLGSSDGMSLNASAPELYELAIMRGEAQVA
                     EGGSLVVSTGSHTGRSPKDKFIVRDGSTESTIWWDNNRAMTRAHFQALKQDFLVHARN
                     SNLFIQDLIAGADPLHELPARVICENAWHALFIQHLLIAPASGKAFTPRLTIIDLPSF
                     KADPQRHGTRSQTVIAFDLANGLVLIGGTQYAGEIKKSVFTYLNYLLPEKGVMPMHCS
                     ANMGAANDTTIFFGLSGTGKTTLSADATRTLIGDDEHGWSDRGVFNFEGGCYAKVINL
                     SAEAEPEIFATTKRWGTVLENVVMDPKTRKLDLHDNALTENTRAAYPLNYIPNASASG
                     QASVPRNIVMLTADAFGVLPPLAKLTPQQAMYHYLSGYTAKVAGTEQGVVEPMATFST
                     CFGAPFIPRHPSVYGNLLRELIATHNVTCWLVNTGWTGGAYGEGHRMPIKATRALLAA
                     AMSGDLDDAEFRTDPHFGFAVPTAVEGVDAKILNPRDTWADKNAYDAQARKLVAMFVK
                     NFDTFKDHVGADIMDASPQMAMAAE"
     gene            5246..5641
                     /gene="rnk"
                     /locus_tag="PKV23_00915"
     CDS             5246..5641
                     /gene="rnk"
                     /locus_tag="PKV23_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HQX84020.1"
                     /translation="MTTAEPRPPILIAEEDRQQLHKLAQSALNHIPEVADELLFDLER
                     AEFRPSPDLSADIVRMNSTVEFETDKGTRLVLQLVYPEKADIAAGRISILTPIGTALI
                     GLSIGQTMTWVDRDGQSRQLRIVAVTGHC"
BASE COUNT         1149 a         1843 c         1555 g         1244 t
ORIGIN      
        1 cgcttcgtcg ggttgcagaa tttttacaaa cagttcttcg gcaacgacca ggtgcatttc
       61 cttggcaaga ccgattccat ggggattttc ggcaccgtct tctttgttgt cgtggccgcc
      121 gcgcttcttt ggtggctata ccgctccctg aaaatcacaa gctgggtcgg catgctgggc
      181 cgcataatct cggccgtgat ggccattttc atcgcctggg tcgtcgccgc ctcgctggtt
      241 tccggcaaca ccattggcac gctgcttaca acgctctttt atgtttttgc tggctgcgcc
      301 ctgcaattcg tgattggaac cggcctcgcc tttctctgct cgcagccgat cctgggaaag
      361 aatttcttcc gcgtgatctt cttcattccg ctgatggtga cgccgctcgg cgtgggctac
      421 gccatgaaaa tggtggccga tgtcacgaag ggtcccttcg agcctctgct caggggcttt
      481 ggcatcgagg actgggtttg gtccgccgac ccctgggcgg cgcgttttgt catgatgatc
      541 tgcgattcct ggcaatggat ccccttcatc ttcatctgca tgctggcagc ccttgagaac
      601 gttccgcgcg accacgtgga ggcagccgag gttgacggcg cttcaagcat ccagattttc
      661 cgcgaagtca cctggccgca gatcgtgccg gttgccgtca ccgtgctcct gatccgcatg
      721 atcgaggcct tcaagatcgt ggatctgcca aacatcatga cgggaggcgg gccaggttcc
      781 tcaaccgaat ccatgacgct tcattcactc tacatctggc gctccaatga catcggcacc
      841 tcggcggcgg tcgcctatct tctgctcatc ctcacggttg tcgtctgcgc gtccctgttc
      901 aactatgtgg tgctcgggca attgcggaag gcgcgcgcat gagcgaggcg tctttcaagc
      961 tcgaaaaggc caaccgcagc ctcatggaac ggctgttcga gccgcccgcc gtcggcaaga
     1021 tgtcgccggt tgctaacctg gttgcttatc ttcttctctt cgtctggtcg atcttcgtgc
     1081 ttttcccgct gtattgggtg gtcatcacat ccttcaagga tgcggccgcg gtaaaccagg
     1141 gcccctatta cattccgttt gtggattttc agccgaacct caatgcctgg cgcgcccagt
     1201 tcaacaccga tcccttctgc gacatgccat caatactccg ccaggtcggc ctactgttcc
     1261 acaatagctt tgcgttcctg gtctcgcccg taatgacgat ccagccgatg gaaccccaga
     1321 tctgcaagat ctacctggcc ttcacaaatt ccatcgtggt cagtgtggcg gccaccatcg
     1381 tatgcgtcac cattggctcc atggcagcct atgccctggc ccgtgtgcag tacaaaccca
     1441 agttcggcaa tatcatgatg ttcgtggcgc tgaccgtgct ggtgattgcc gccacaagct
     1501 atgccggtgt tccctggtgg gcctccaccg ccgtggccct tgccctgttc ttctttctcg
     1561 cccgcgcctt tggcaaatac ttcacccgca ccttggccaa tggcgacatc ctgttctgga
     1621 tcatctcgca gcgcattctg ccgcccatcg tcgtgataat tccgatttac gtgatgttcc
     1681 aggccgtagg ccttctcgat actcacctgg cgcttatcac agtctatgcg gtggccaatt
     1741 tgcccatcgt cgtctggctc atgtatgatt ttttctccag cctccccatc gaactcgagg
     1801 aatcggccca gctcgacggc gccacgcgct ttggaatttt ctgggaaatc gttttgccgc
     1861 tgacacggcc aggcctcgcg gccacaacgc ttctcacgct catcctgagc tggaacgaat
     1921 atctgttcgc ggttttcctt tccacctcca aggtgcagac aatgcccatc atggtgcagg
     1981 ccaagaatgc cggcgagaag ggcgtcttct ggtgggagat gtgcgccatc atcgtggtga
     2041 tgatcgtccc cgtcatcatc atggccatca tccttcaacg tttcattgcc aggggcgtgc
     2101 tccttggcgc agtcaaagga tagagagtaa cagagatggc aagcggcaag accggagttc
     2161 cggtttcctt cgacagaata cacaaatcct tcgggccggt gcgcgtcctg gaggagctga
     2221 acttgcaggt cgagcccggc gaattcctgg ttcttctcgg cgcctcgggg tcggggaaaa
     2281 caacagcgct gcgcattctc tccggccttg aaaccgcaac cgccggccgc gtgctgatcg
     2341 gcaaccggga cgtgaccgat atcttgccga aataccgcga catctccatg gtgttccagt
     2401 cctatgcgct ctatccgcac aagaccgtgg ccgaaaacat tggcttcccc ttgaaggtgc
     2461 gcaaggttcc gcaagccgag caagacgtgc agatccgcga tgcggccgcc caggtgcagc
     2521 tcggcgagtt gctgcaccgc tatccgcgcg agctctccgg cggccagaag cagcgcgtgg
     2581 cactggctcg cgccatcatc cgccgcccct cggtcttcct tatggatgag ccgctctcga
     2641 atctcgacgc caagctccgc ggccacatgc gcgccgagct gaagcacatg caggaaagct
     2701 tcggcatcac cacgatctac gtgacccacg accagatcga ggccatgacg cttgcccgcc
     2761 gtgtggccat acttgagcgc ggggtgctgc agcaactcgc cacaccggct gaaatctaca
     2821 acaacccggc caatctcttc gtcgcccagt tcatcggctc gccgcccatg aacgtggtcc
     2881 agggcgccct tgagggaaag aattttctca acaatggtgc caagatcgcg accccggtga
     2941 gcggcaggat tgccaaggcc gtgctgggcg tgcggccgga ggattgctcc gtggtggcgc
     3001 cggagaaggg cgggatcaag ggcgaaattt atgctaccga gctgatcggc gaccacacgc
     3061 tggtaaccct gaaggcgggc agcgacctgc tcaccgtcaa ggcggccaag gacttcaccg
     3121 ccaggctcgg cgaaaaggcg ggcgtggctt tcagcagcga ccgcctgttt gtgttcgacg
     3181 aaagcaatgg cctgcgggtc cgctgaaccc tcacctcaac cagaataggg ctatctatca
     3241 aaagcttgaa tccttctccc gaagctcgga acgctcaggt ttcgttaaga aatgatgtga
     3301 aaacatcata aaatgacttt cgataccgca agtaattgac gataacacat catttttctt
     3361 gatcaaggcg cggcattttg ttagcgatga gtcttccatt tcaggggaat gaccatgcag
     3421 attgacagca ccacgcgctc ccagctcctc agcctcggcc tcggctcgtc cgatggtatg
     3481 tcgttgaacg ccagcgcccc tgaactctat gaacttgcca tcatgcgcgg tgaagcccag
     3541 gtcgccgaag gcggcagcct cgtggtcagc accggcagtc acaccggacg cagtccgaaa
     3601 gacaaattca ttgtccgcga cggttccacc gaaagcacca tctggtggga caacaaccgc
     3661 gccatgaccc gggcccattt ccaggcgctg aagcaggatt tccttgtcca cgccagaaac
     3721 agcaacctct tcatccagga tctcattgcc ggggctgatc cgctccatga attgccggcc
     3781 cgcgtcatct gcgaaaatgc ctggcatgcc ctgttcatcc agcatctgct gatagcacct
     3841 gcaagcggca aggcattcac cccccgcctc accatcatcg acctgccatc cttcaaggcc
     3901 gacccgcagc gccatggcac ccgcagccaa accgtgatcg cctttgacct tgccaatggc
     3961 ctggtactca tcggcggcac gcaatacgcg ggcgagatca agaaatccgt tttcacctat
     4021 ttgaattacc tgctgcctga aaagggcgtg atgcccatgc attgctcggc caacatgggg
     4081 gccgccaatg acaccacgat cttcttcggc ctttccggca ccggcaagac aacgctttcc
     4141 gccgatgcca cccgcaccct tatcggcgat gatgagcatg gctggtcgga ccggggcgtc
     4201 ttcaacttcg aaggcggctg ctatgccaag gtgatcaatc tttccgccga agccgagccg
     4261 gaaatcttcg cgacaacgaa gcgctggggc accgtgcttg aaaatgtcgt gatggatccg
     4321 aaaacccgca agctcgatct ccatgacaat gcgctgaccg aaaacacccg cgccgcctat
     4381 ccgctgaatt acattccaaa tgccagcgca tcaggccagg ccagcgtgcc aaggaacatc
     4441 gtgatgctca cggcggacgc cttcggtgtg ctgccgccac tggcaaagct cacgccgcaa
     4501 caggccatgt atcactatct ctctggctac accgccaagg tggcgggcac cgagcagggg
     4561 gtggtggaac cgatggccac cttctccacc tgcttcggcg cgcccttcat tccgcgccat
     4621 cccagcgtct atggcaatct gcttcgcgag cttatcgcca cgcataatgt cacctgctgg
     4681 ctggtgaaca ccggctggac cgggggcgcc tatggtgaag gtcaccgcat gcccatcaag
     4741 gccacccggg cgctccttgc cgccgccatg agcggggacc ttgacgacgc ggaattccgc
     4801 accgacccgc atttcggctt tgccgttcca accgcggtgg aaggtgtgga cgcgaaaatc
     4861 ctgaacccgc gcgacacctg ggctgacaag aatgcctacg acgcccaggc ccggaaactc
     4921 gtcgccatgt tcgtgaagaa cttcgacacc ttcaaggacc acgtcggcgc cgacatcatg
     4981 gacgccagtc cgcagatggc aatggcggcg gaataggatt acaccgcatc ttgaattctg
     5041 ctttgaaaag actacatgtc aggacatgcg tagctcattg tcaaaccttg tgatcgaaac
     5101 ccgcctcgct tttggctaga cagcctcggc gcgcctgtgg gcgttcgccc gaggcttaac
     5161 cccgcatctc gtccatcaaa ttcccagaaa ccgccctgga gccgcttcgc cgcgcggggc
     5221 cacatcattt tattggaaac gcatcatgac cacagctgag ccccggcccc cgatcctcat
     5281 tgccgaagaa gaccggcaac agctccacaa gcttgcccag tccgccctca accacattcc
     5341 cgaggttgcg gatgaattgc tcttcgacct tgagcgcgcc gaattccgcc ccagccctga
     5401 cctgtctgcg gatatcgtgc ggatgaattc aaccgttgag tttgaaaccg acaagggaac
     5461 taggctcgtc ctgcagcttg tctatccgga gaaagccgac atcgctgccg gtcgaatctc
     5521 aattctgacg ccaatcggca cggcgcttat cgggctgtcc atcggccaga ccatgacctg
     5581 ggtcgaccgg gacggccagt cgcgccaact caggatcgtt gctgtgacag gccattgttg
     5641 aacggccggt tttctgccat cccggcttgc tcggggtggc taaacctgaa aaagagaacc
     5701 cgggattagc tctgcgacta aggcgcgagc ccatagcagc gccccgttca caccagttaa
     5761 aaaaatcagc taaatttaga atcatcaatt g
//
LOCUS       DAORVN010000025         5720 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_9372_length_5720_cov_3.029126, whole genome shotgun sequence.
ACCESSION   DAORVN010000025 DAORVN010000000
VERSION     DAORVN010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 5720)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5720)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5720
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9372_length_5720_cov_3.029126"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="PKV23_00920"
     CDS             complement(<1..189)
                     /locus_tag="PKV23_00920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HQX84021.1"
                     /translation="MQTADGARRDIVSVNLGQRTYDIHIGENLLQEAGRYIAPLLSRP
                     FAAIVTDENVARHHLKALE"
     gene            complement(176..769)
                     /locus_tag="PKV23_00925"
     CDS             complement(176..769)
                     /locus_tag="PKV23_00925"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020181189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HQX84022.1"
                     /translation="MARRSLSKEIEDIREKLGNHPLVLVGLMGAGKSSIGRRLAEKLE
                     VPFVDADHEIEIAADKSIPEIFKDHGEAYFREGERRVISRLIENGAQVLSTGGGAFIN
                     EETRQAVQARGISVWLKADLPLLMKRVMKRADRPLLQTGDPEAVMRKLMDQRYPIYAT
                     ADVTVESRDVQHTQMVNEVIKVLAKWPGWERLNNANS"
     gene            891..1034
                     /locus_tag="PKV23_00930"
     CDS             891..1034
                     /locus_tag="PKV23_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004679604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HQX84023.1"
                     /translation="MPDSLRFLFVVLILAGAVYGAVWGLANFPPEQSEVVKSLPHEKL
                     RQR"
     gene            1012..1971
                     /locus_tag="PKV23_00935"
     CDS             1012..1971
                     /locus_tag="PKV23_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009450788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HQX84024.1"
                     /translation="MKNSGSDKTARASPSHRHVDRFLEMMSAERGAAENSLQAYERDL
                     QHYAAFLEARGGALLTATSDDIRSYLASLEDHGFARTTAARRLSAVRQFHQFIHGEGL
                     VAGNPAAIVESPKAGRPLPKTLSVQDIDLLLAEARARVSRAEGKGRFKAERLYCLLEL
                     LAATGLRVSELVGLTVRAVTADDAFLAIKGKGGRERLVPVSDRARGVLQGYLKTLAAR
                     EAAETKWLFPSHGASGCLTRQHFAIELKALAAACGLDLGKVSPHVLRHAFASYLLECG
                     ADLRAVQQMLGHADISTTQIYTHVQAGRLQAVVEIHHPLAKKS"
     gene            2041..2991
                     /locus_tag="PKV23_00940"
     CDS             2041..2991
                     /locus_tag="PKV23_00940"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019995787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HQX84025.1"
                     /translation="MIYLDFETSVTELEGKIAELRALAAGDATVSIADEVKALEKKAN
                     KSLADIYVNLTPWQKTQVARHPDRPHALAYINNLITDFVPMAGDRKFADDQAVVGGLG
                     RFRGSSVVVIGQEKGADTQARLKHNFGMARPEGYRKAVRLMELAERFGIPVITFVDTA
                     GAYPGIDAEERGQAEAIARSTDCCMGLGVPLVSLIIGEGGSGGAIAIAAGNSVLMMEH
                     SVYSVISPEGAASILWRDAGRAKDAAAALKITAQDLKRLGVIDDIVNEPLGGAHRQPD
                     QAIASAGEMIGRILEAFAGVSPDVLRTQRREKFLAIGRSI"
     gene            complement(3028..3207)
                     /locus_tag="PKV23_00945"
     CDS             complement(3028..3207)
                     /locus_tag="PKV23_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008136883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HQX84026.1"
                     /translation="MKNAKYLAKLFVRDASGATAIEYGLIAAAMGLMLIPVMFSLSST
                     VKVTLFDVMVGLFPT"
     gene            3338..4729
                     /locus_tag="PKV23_00950"
     CDS             3338..4729
                     /locus_tag="PKV23_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012631107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase family protein"
                     /protein_id="HQX84027.1"
                     /translation="MNKRLSRRAVFQGIFAAPFAAGLVRAQTPSELRTVSRIIEVNGR
                     AAKVFGIVNGAGGHGLSLVLGERFRARVTNGLEVETLLHWHGLNPPSAQDGVPMLSQA
                     PLKPGQSFDYDFVNTRSGTHWMHSHVGLQEQQLLAAPLIVRETAEPLFDEQEHVVLLH
                     DFSFREPAEILAELRSGGGHAGHAMSGAEMAMPGMLNDITYDAYLANDRTLADPEVVR
                     VGKATQIRLRIINGAAASNMWIDLGALQGELIAVDGNAVYPLKGSLFPLAIAQRADIR
                     VKLPEGNGAWPILFRPEGTAARTGIYLATQGAEITRLSTAGEMTPAVDVAQEMKLRSV
                     ADLPPEPVTRTEILALTGGGGDYLWGLNGKSSMHDTIFTVRENERIEVVMLNQTGMAH
                     PMHLHGHYFKVAAINNVAIEGALRDTVLVPPSSSVTIRFAADNPGNWAFHCHHLYHMN
                     AGMMGAIRYSNAA"
     gene            complement(4735..>5720)
                     /locus_tag="PKV23_00955"
     CDS             complement(4735..>5720)
                     /locus_tag="PKV23_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013167582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HQX84028.1"
                     /translation="YLSLEDDLMRIFGSERMDSMLQKLGLKEGEAIVHSWINKALEKA
                     QQKVEARNFDIRKNLLKFDNVMNDQRKVIFEQRIGIMKGEDVSETVDDMRHQVIDDLI
                     TKYMPPNTYAEQWETAELRQRLREAVAIDFPIEDWAKEEGIADEEIRERVRKAADELY
                     QAKREKFTPAIMQQVEKAVVLQTLDQLWRDHIVTVEQLRQVIHLRGYGQRDPLNEYKT
                     EGFNLFEAMISKLRETATAQVMRVEIQSRAPGELLPDEDDLPFMEAHHIDATSGQDDV
                     GEGYPVFGQATAGAAATLDPKRPETWGKVSRNEPCPCGSGKKYKHCHGTFV"
BASE COUNT         1100 a         1621 c         1767 g         1232 t
ORIGIN      
        1 ttctagggcc ttgagatgat ggcgcgccac gttttcatca gtgacgatgg cggcgaacgg
       61 gcgggaaagg agcggcgcga tatagcggcc cgcttcctgc aacaggttct cgccaatatg
      121 gatgtcatag gttctctgcc cgagattcac cgatacgatg tcgcggcgcg cgccgtcagc
      181 tgtttgcatt gttcagacgc tcccagcccg gccacttggc caggaccttg atgacttcat
      241 tgaccatttg cgtgtgctgc acgtcgcggc tttcaacggt tacgtcagcg gtagcgtata
      301 tgggatagcg ctggtccatc agcttccgca tgacggcctc gggatccccg gtttgcagca
      361 agggacggtc ggcgcgcttc atcacgcgct tcatcagcag gggcagatcg gccttcagcc
      421 aaaccgaaat gccgcgggcc tggacggcct ggcgggtttc ctcattgatg aatgcacccc
      481 cgccggttga cagaacctgc gcgccatttt caatcaggcg ggaaatcacc cggcgttcgc
      541 cttcgcggaa ataggcttcg ccgtgatcct tgaaaatttc agggatggac ttgtcggcgg
      601 caatctcgat ttcatggtcg gcatcgacga aggggacctc aagcttttcc gccagcctgc
      661 gaccaatgct ggatttgccg gcgcccatca ggccgacaag cacgagcgga tgatttccga
      721 gcttttcccg gatatcttca atctccttcg agagcgaacg ccgcgccatt attcccttcc
      781 ctgtgctggc ggagctttta gaacggcggg gtcagagcgc caagactttt gatttggtgg
      841 ccatttgccg gcatctggga taacctgcca ggtgatttgg agatttgcgc gtgcctgaca
      901 gccttcggtt cctgtttgtc gttctgatcc ttgccggcgc tgtctatggc gccgtgtggg
      961 ggctggcaaa ttttccacca gagcagtcgg aagtcgtgaa atccttaccc catgaaaaac
     1021 tcaggcagcg ataagacagc gcgggcttca ccgtcgcacc ggcatgtgga ccggttcctc
     1081 gagatgatga gtgccgagcg cggggcggcg gaaaattccc tgcaggctta cgagcgcgat
     1141 ctccagcact atgcggcctt tcttgaggcc cggggcggag cgctcctgac ggctacgagc
     1201 gatgacatcc ggtcctatct ggcaagcctt gaagaccatg gctttgcccg cacaacagcg
     1261 gcgcggcggc tttcggcggt tcgccagttc caccagttca ttcacggcga aggcctcgtt
     1321 gctggcaatc cggcggccat cgtggagagc ccgaaggcgg gccggccgtt gcccaagacg
     1381 ctttcggtcc aggatattga cctactgctt gccgaggcgc gagcccgggt ttcacgggcg
     1441 gaaggcaagg ggcgtttcaa ggcggaacgg ctttattgcc tgctggagct tctggcggcc
     1501 acgggccttc gcgtttccga acttgtcgga ctgacggtgc gggcggtgac cgcggatgat
     1561 gcctttctgg cgatcaaggg caagggcgga cgggaacggc tcgtgccggt atccgaccgg
     1621 gcgagaggcg ttctccaggg ctatctcaaa accctcgcag cgcgcgaggc ggctgaaaca
     1681 aaatggctgt ttccgtcgca cggggcaagc ggctgcctga cccgccaaca tttcgccata
     1741 gaacttaagg ccctcgcggc ggcctgcggc ctcgatcttg gcaaggtttc gccccacgtg
     1801 ctgcgccacg cctttgccag ttatctgctg gaatgcgggg ccgacctccg ggcagttcag
     1861 caaatgctgg ggcacgcaga tatttcgaca acccagatat acacccatgt ccaggcgggg
     1921 cgcctgcagg cagtggtgga gattcaccat cctttagcta agaaaagttg actttatccc
     1981 ctccggcggt cacatctggc gcacccaaaa gccacttcat tcactaccct ttgaggacgt
     2041 atgatctatc tggactttga aacctcggtt accgaacttg aagggaagat tgccgagctg
     2101 cgcgcccttg ctgccggaga cgccacggtt tcaatcgccg acgaggtcaa ggctttggag
     2161 aaaaaggcga acaagagcct ggctgatatc tatgtcaact tgacgccctg gcaaaaaacc
     2221 caggtggccc ggcacccgga caggccccat gcgctggcct atataaacaa cctgatcacc
     2281 gattttgtgc cgatggcagg tgaccggaaa tttgccgacg atcaagccgt tgtcggcggc
     2341 ctcggacgct tccgcggttc gtcggtggtg gtcatcggcc aggagaaagg ggctgacacg
     2401 caggcccgcc tcaagcataa tttcggcatg gcgcggccgg agggatatcg caaggccgtg
     2461 cggctcatgg aactggccga acgcttcggc attccggtga taacctttgt ggatacggcg
     2521 ggcgcctatc cgggcattga tgcggaagag cgcggccagg ctgaagcgat tgcgcgatcg
     2581 actgactgct gcatgggtct tggcgtgccg ctggtttccc tgatcatcgg cgagggcggc
     2641 tcgggagggg ctatcgccat tgcagcggga aacagcgtgc tgatgatgga gcattcggta
     2701 tacagcgtca tctcgccgga aggggcggcc agcatcctgt ggcgtgatgc gggaagggcc
     2761 aaggacgcgg cggcggccct gaagatcacg gcgcaggatc tcaagagact gggcgtcatt
     2821 gatgatatcg tgaacgagcc gctaggcggc gcgcaccgcc agccggatca ggcgatagct
     2881 tcggcaggtg aaatgatcgg acggatcctg gaagcttttg ccggcgtctc acccgacgtg
     2941 ttgcggacgc agcggcgcga gaagtttttg gcgattggcc gcagtatcta aagtgcgatt
     3001 cagccccgat gatccgtcgc gaaaagctta tgtcgggaaa agacctacca taacgtcaaa
     3061 caatgtcacc ttgacggtag aagacagcga gaacatcacg ggaattaaca tcaatcccat
     3121 ggcagccgca atcaagccat actcaatggc ggtggctccg ctggcgtcgc gaacgaaaag
     3181 ctttgctaga tatttcgcat tcttcattgt ggcaaccctg cataatgagt cattcgctgt
     3241 catgccagaa cttaatcctg acttgttttc agaatgttaa ttcattgtct tcgtttgtat
     3301 tcatcccctt ttgaggcaac cctttcggcg agtttagatg aacaagaggt tatcgagacg
     3361 ggcggttttt caaggtattt tcgccgcgcc ctttgcagca ggccttgtcc gggcgcaaac
     3421 acccagcgaa ctccgaactg tttcacggat tattgaggtt aacggccgcg cggccaaggt
     3481 tttcggcatc gtgaatggcg cgggcggaca cgggctttcg ctggtgctcg gggaaaggtt
     3541 ccgggcgcgc gtcacgaacg gcctggaggt tgaaacactt ttgcactggc acggattgaa
     3601 cccgccttcc gcccaggacg gtgttcccat gctgtcgcag gcgccgctga aacctggcca
     3661 atcctttgac tatgattttg tgaatacgcg ttccggcaca cactggatgc attcccatgt
     3721 gggacttcag gaacagcagc ttctggcggc accgctgatt gtgcgggaga cggcggaacc
     3781 cctgtttgac gagcaggagc atgtggtgct gctccatgat ttctcctttc gcgagccggc
     3841 ggaaattctg gccgagctca ggagcggcgg cggccatgcg ggtcatgcga tgagcggcgc
     3901 ggaaatggcc atgccgggca tgctcaatga catcacctat gacgcctatc tggccaatga
     3961 ccgcaccctt gccgacccgg aagtggtgcg tgttggcaag gccacgcaaa tccgcttgcg
     4021 gatcatcaat ggcgcggcgg ccagcaacat gtggatcgat ctgggggcac ttcagggcga
     4081 acttatcgcg gtcgacggca atgcggtcta tcccctcaag ggttccctgt ttccgctggc
     4141 catcgcgcaa agggcggata tccgcgtgaa actgccggag ggcaatggcg cctggcccat
     4201 tcttttcagg ccggaaggaa cggccgcaag aaccggcatt tatcttgcga cccagggcgc
     4261 ggaaatcacc aggctttcaa ctgcgggtga gatgactccg gcagtggatg tggcccagga
     4321 aatgaagttg cgctcggtcg ccgacttgcc gcccgagccg gtgacgcgca cggaaatcct
     4381 ggcgctgacc gggggcggcg gcgattatct ctggggactt aacggcaagt catccatgca
     4441 tgatacgatc ttcacggttc gcgaaaatga acgcattgag gttgtcatgc tgaaccagac
     4501 cggaatggcg catcccatgc atctgcacgg gcattacttc aaggttgcgg ccatcaacaa
     4561 cgtggccatc gaaggggcgt tgcgggatac ggttctggtt ccgccctcga gttcggtgac
     4621 catcaggttt gcggcggaca atcccggcaa ctgggccttc cactgccacc atctctacca
     4681 catgaacgcg ggcatgatgg gcgccatcag atattccaac gcggcttgat gggcctagac
     4741 gaacgtcccg tggcagtgct tgtacttctt tccggagccg cagggacagg gttcgttgcg
     4801 tgaaaccttg ccccaggtct ccggccgttt tgggtcaagc gtggcggcgg cgccggctgt
     4861 tgcctggccg aagaccgggt aaccctcgcc aacatcgtcc tggccggagg tggcatcgat
     4921 gtggtgggct tccatgaagg gaaggtcatc ctcatcgggc aagagttcac ccggggcccg
     4981 tgattgaatc tcaacccgca tcacctgggc ggttgcggtt tcgcgcaatt tggagatcat
     5041 ggcctcgaag aggttgaagc cttcggtttt gtattcattg agcgggtcgc gctggccata
     5101 gccgcgcaga tggatgacct ggcgcaactg ttctaccgtc acaatgtgat cgcgccagag
     5161 ctggtcgagg gtttgcagga ccaccgcctt ttcaacctgc tgcatgatgg ccggggtgaa
     5221 tttctcgcgc ttcgcctgat agagttcatc ggccgccttg cgcacacgct cgcggatttc
     5281 ctcgtcggcg atgccttctt ccttggccca gtcttcaatg gggaaatcaa tggccacggc
     5341 ttcgcgcagg cgctggcgca gttcggcggt ttcccattgc tcggcgtagg tgttgggggg
     5401 catgtatttt gtgatgagat cgtcgatgac ctgatggcgc atgtcgtcca cggtttcgga
     5461 cacgtcttcg cccttcatga tgccgatgcg ctgttcaaag atcaccttgc gctggtcgtt
     5521 catcacgttg tcaaatttca ggaggttctt gcggatgtcg aagttgcgcg cctcaacctt
     5581 ctgctgggcc ttttcaaggg ccttgttgat ccaggaatgg acaatggcct cgccttcctt
     5641 gaggcccagc ttctggagca tggaatccat gcgctctgaa ccgaaaatgc gcatgagatc
     5701 atcctccagg gagagataga
//
LOCUS       DAORVN010000026         5587 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_9767_length_5587_cov_2.546999, whole genome shotgun sequence.
ACCESSION   DAORVN010000026 DAORVN010000000
VERSION     DAORVN010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 5587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5587
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9767_length_5587_cov_2.546999"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..300
                     /locus_tag="PKV23_00960"
     CDS             <1..300
                     /locus_tag="PKV23_00960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HQX84029.1"
                     /translation="EGSLVWQDTMAIPTGAPHPENAHAFLNFVLDAKAGAHIADTIKF
                     ATANAAAKAIMDKSYTENTSIFPPAEIVAKCEPGLYLGEEATKVRDEIWTRVQAA"
     gene            398..1270
                     /locus_tag="PKV23_00965"
     CDS             398..1270
                     /locus_tag="PKV23_00965"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012110457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQX84030.1"
                     /translation="MDNWKDNRSVFWTLAGPGTFWLLVFFILPLGFLFVMSFSDKAII
                     NGEVSITEYDYLTGISNYIRSVDTLYLTIIWKSIWVSALATVLCLLTAYPIAFGICFA
                     SEKWKPLLLLLVVLPFWINLLIRTYALIAVFRSQGYVNLALGAVGLGPYDMLYNNFAV
                     IFGLVYVYMPFMVLPLYATIERLDKSYLEASLDLGASQFTTFLKVTIPLTMPGIITGV
                     ILVFIPCLGSFLTPDLLGGANAIMIGNVIERQFKAANDWPFGAALSFMLMYATFAALM
                     LRAFLAKRSAGVDV"
     gene            1270..2118
                     /locus_tag="PKV23_00970"
     CDS             1270..2118
                     /locus_tag="PKV23_00970"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012110456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQX84031.1"
                     /translation="MAEQMKPGSGPLDYGNRLWLKVLFGVIFFMLYVPIITLVAFSFN
                     TDRRGIVWRGFTFDNYVKAWNNSSLIEAMINSLMIAFIATLIATVLGAMVALMLWRFR
                     FPFKGAYEAFMGLPIVIPEICMGVALLLFFNETGMMTAVVAMPWPLNLSNIIIAHVAF
                     CFPFVAIVVRSRLVGFNRQLEEASKDLGASEWQTFWKVIVPFMMPGLVAGALLAFTLS
                     LDDFVITFFTSGPETVTFPVKVYSLVRRGVSPEINAASTVLIVITVVATVLAMKLQAP
                     QKSGKG"
     gene            2121..3209
                     /locus_tag="PKV23_00975"
     CDS             2121..3209
                     /locus_tag="PKV23_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018234767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQX84032.1"
                     /translation="MTDPIVSIRGVSKKFPGGVIAVDDVNIDIAPNEFFALLGPSGCG
                     KTTLLRMISGLETPTDGQIIIGGQDMALTPPNKRPTNMVFQSYAVFPHMTVEDNVAYG
                     LRVTGVPEDETKRRTAEALEMVKLSHLAKRKPDQMSGGQRQRVALARALVKRPKVLLL
                     DEPLSALDAKLRDDMRMELTRLQETVGITFIIVTHDQDEALSMASRIAVIDKGAVQQI
                     ATPSELYEQPATRFVADFIGKVNLIDAKVLSKKAKAITCEAKGLGKLTFDTEKACGDT
                     VAVAVRPEKLKISKAEPKAAGLIKVQGNVRDVAYYGDTSHIVVSIKGGPDLQVNVQND
                     SRTGGSGVERGQAIWVSWMPDDSLVLTE"
     gene            3213..4208
                     /gene="speB"
                     /locus_tag="PKV23_00980"
     CDS             3213..4208
                     /gene="speB"
                     /locus_tag="PKV23_00980"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008192603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HQX84033.1"
                     /translation="MALELDKMTRLPGDEAFRRETIKGTQWESTYAGALSFMRRKYTR
                     DLTGVDIAVTGIPFDQAVSHRPGTRFGPEGMRKASAENAWGPFWPWMFDPFNTLAVID
                     YGDCFFDWGRKENFPERLEAHAAEIISSGAEMVSLGGDHYITYPLLKAHFKKHGPLAL
                     VHFDAHRDVEVDDGGRIDHGTMFGYAVREGLIDPRKSVQMGIRTTFTGETTMGFRIVY
                     ADEVHDSSSGQLAAIIKKQVGKSKAYLTFDIDCLDPSVAPGTGTPIAGGMTYHQAASV
                     IRKLTDVNFVAMDVVEVSPPYDHADITSIAGASLVMEYLCLKAWQRGARATPMPE"
     gene            4205..4702
                     /locus_tag="PKV23_00985"
     CDS             4205..4702
                     /locus_tag="PKV23_00985"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HQX84034.1"
                     /translation="MKLVPELPEHRPAVLELNRLAFKGDEEPRLIKNLARGNSIFASF
                     VALDGEEVVGHIMFSPLHLVVNGERIDAVALAPMCVTPGRQKQGIGTQLVDYGIEQMK
                     RAGQAAIIVLGHVDFYPRFGFRHDLARNLSCAFNKYDAFMGLELRPGSLDGKSGTCIY
                     PRAFD"
     assembly_gap    4817..4826
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4827..>5587
                     /locus_tag="PKV23_00990"
     CDS             <4827..>5587
                     /locus_tag="PKV23_00990"
                     /inference="COORDINATES: protein motif:HMM:NF014748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyramine oxidase"
                     /protein_id="HQX84035.1"
                     /translation="ETIALHEPEKAELAQGNPPRLAFVCCYDPHTGQTWDGIADLESG
                     LLRNWRHVAGAQARIVSDEFVMGGEVAKADPRVIAALALRGITDLTGVLIEAWSAGNF
                     GIAEEQGERIAYCHCFLRNTAGDNPYERPIANLHPVVDLRRRIVTRVDDFGVVPLPPD
                     GKPLRQESLRTDLKPLQITQPEGPSFTVEGNLVRWQKWQMRVGFHVRDGLILHMIGYE
                     DEGRLRPIMHRASMAEMVVPYGDPRGGAYRRNAFDT"
BASE COUNT         1095 a         1744 c         1539 g         1199 t
ORIGIN      
        1 gaaggctcgc tggtctggca ggacacaatg gcgatcccca ccggtgctcc ccatcccgaa
       61 aacgcccatg ccttcctgaa cttcgttctt gacgcaaagg ccggcgcgca cattgccgac
      121 accatcaagt ttgccactgc aaacgcggcg gccaaggcaa taatggacaa gtcctatacg
      181 gaaaacactt cgatcttccc gcctgcggaa atcgtggcga aatgcgaacc cggcctctat
      241 ctcggcgaag aggcaaccaa ggtgcgcgac gaaatctgga cacgcgttca ggcggcctga
      301 taacgtcctg agaggtttgc ggggacggca gcttgccgtc cccgttctta ttggctagca
      361 ttcagggatc gcaggggcag gggagagaag gcgcgtcttg gataactgga aagacaacag
      421 aagcgtcttt tggaccctcg ccgggcccgg caccttctgg cttctcgttt tcttcatttt
      481 gcccctgggc ttcctctttg tcatgtcctt cagcgacaag gcgataatca acggtgaagt
      541 gtccatcacc gaatatgatt acctgaccgg catatcgaac tatatccgca gcgttgatac
      601 gctctatctc accatcatct ggaaatcgat ctgggtaagt gcgctcgcca ccgtgctgtg
      661 cctgctgaca gcttacccca tcgctttcgg aatctgtttt gcctctgaaa aatggaagcc
      721 tctccttctg ctgcttgtgg tgctgccgtt ctggatcaat ctcctgatca ggacctacgc
      781 attgatcgcc gtgttccgct cgcagggcta tgtgaatctg gctttgggcg ccgtggggct
      841 cggcccctat gacatgctct acaacaattt tgccgtcatc ttcgggctgg tttatgtcta
      901 catgcccttc atggtgctgc cgctttacgc caccattgag cgcctcgaca aatcctacct
      961 tgaagcaagc cttgatctcg gcgcctcgca attcaccaca ttcctgaaag tcaccattcc
     1021 cctcaccatg ccgggcatca ttaccggcgt tatccttgtc ttcattcctt gtctcggctc
     1081 gttcctgacg ccggatcttt tgggcggggc caacgcgatc atgatcggca acgtcatcga
     1141 gcgccagttc aaggcggcca atgactggcc ctttggcgcc gcactgtcct tcatgctgat
     1201 gtacgccacc tttgccgcct tgatgctgcg ggcctttctg gccaagcgca gcgctggagt
     1261 tgatgtgtaa tggctgagca gatgaaaccc ggctccggcc cgctcgacta cggcaacagg
     1321 ctgtggctga aagtcctttt cggcgtgatc ttcttcatgc tctatgtccc catcatcacg
     1381 ctggttgctt tttccttcaa tacggacagg cgcggcattg tctggcgcgg cttcacattc
     1441 gataactacg tcaaggcctg gaacaacagc tcactcatag aagcgatgat caattccctg
     1501 atgatcgcct tcattgccac cctgattgcc acggtgcttg gcgccatggt ggccctcatg
     1561 ctgtggcgct tccgtttccc cttcaagggc gcctatgagg cgttcatggg gctgcccatt
     1621 gtcatccccg aaatctgcat gggcgtggcg ctgctcctgt tcttcaacga aaccggcatg
     1681 atgaccgcgg tggtggccat gccatggccg ctcaatctct ccaatatcat cattgcccat
     1741 gtggctttct gcttcccctt cgtggccatc gtcgtgcgct cgcgccttgt cggcttcaac
     1801 cggcagctcg aggaagcctc gaaggatctg ggcgccagcg agtggcagac cttctggaag
     1861 gtgatcgtgc ccttcatgat gcccggactg gtggccggcg cgctgcttgc cttcacgctt
     1921 tccctcgatg acttcgtgat caccttcttt acctcgggcc ccgaaaccgt gaccttcccg
     1981 gtcaaggtct attccctcgt ccgccgcggc gtctctccgg agatcaacgc ggcctccacc
     2041 gtcctgattg tgattaccgt ggtggcaacc gttctcgcca tgaaactcca ggctccacag
     2101 aaatctggca aaggttgaat atgacagacc caattgtatc cattcgcggc gtcagcaaga
     2161 aatttccggg gggagtcatt gccgtcgatg atgtcaacat tgatatcgcg ccgaatgaat
     2221 tcttcgcgct gctcggcccc tcgggctgcg gcaagaccac cttgctccgc atgatctcag
     2281 ggctcgaaac gccaaccgat ggccagatca tcattggcgg ccaggacatg gcgctgaccc
     2341 cgcccaacaa acgcccgacg aatatggtgt tccagtccta cgccgtgttc ccccacatga
     2401 ccgtggaaga caatgtggcc tatggcctcc gggtgactgg cgtgccggaa gatgaaacca
     2461 aacgccgaac ggccgaagcc cttgagatgg tgaagctctc gcatcttgca aaacgcaagc
     2521 ccgaccagat gtccggcggc cagcgccagc gcgtcgctct agcccgcgcc ctggtgaagc
     2581 ggccgaaagt cctgcttctg gatgaaccgc tgtcggctct ggacgccaag ctgcgcgacg
     2641 acatgcgcat ggaactgacc aggcttcagg aaaccgtggg catcaccttc atcatcgtca
     2701 cccatgacca ggatgaagcc ctgtccatgg ccagccgcat tgccgtcatc gacaagggcg
     2761 ctgtccagca gattgcgacg ccttccgaac tttatgagca gccagccacc cgcttcgtgg
     2821 ccgatttcat cggcaaggtg aacctgattg atgccaaagt cctgagcaaa aaggcaaagg
     2881 ccatcacttg cgaggccaag ggccttggca agctgacttt tgatactgaa aaggcctgcg
     2941 gcgacaccgt ggctgttgct gtgcgccccg agaaactgaa gatctcaaag gccgagccaa
     3001 aggctgcggg acttatcaag gtccagggta atgtgcgtga cgtggcctac tatggcgaca
     3061 ccagccatat cgtcgtatcc atcaagggcg gccctgacct gcaggtgaat gtgcagaacg
     3121 actcgcgcac cggcggttcc ggcgtcgagc gcggccaggc catttgggta tcctggatgc
     3181 ccgacgattc cctcgtgctc acggagtaga tcatggccct cgaacttgac aagatgaccc
     3241 ggcttccggg tgacgaagca tttcgccgcg aaaccatcaa gggcacccag tgggagtcga
     3301 cctatgcggg agcgctctcc ttcatgcgcc gcaaatacac ccgcgatctc accggcgtgg
     3361 acatcgcagt cactggcatc cccttcgacc aggccgtgtc gcacaggccc ggcacccgtt
     3421 ttggcccgga aggcatgcgc aaggcgtctg cggaaaatgc ctggggcccg ttctggccct
     3481 ggatgttcga tcccttcaat accctggccg tcattgatta cggcgattgc ttcttcgatt
     3541 gggggcggaa ggaaaacttc ccggaaaggt tggaagcgca cgccgctgaa atcatcagtt
     3601 ccggcgccga aatggtctcg ctcggcggcg atcattacat tacctatccg ttgctgaagg
     3661 cccatttcaa gaagcatgga cccctggccc tggtgcattt cgatgcccac cgcgacgttg
     3721 aagtcgatga tggcggccgc atcgatcacg gcaccatgtt cggctacgcc gtccgtgaag
     3781 gcctcatcga tccgcgcaaa tcagtgcaga tgggcatccg caccaccttc accggtgaaa
     3841 ccaccatggg cttccgcatt gtctatgccg atgaggtcca cgactcgagc agcggccagt
     3901 tggccgccat catcaagaag caggtgggca agtcgaaagc ctatctcacc tttgacattg
     3961 attgcctcga tccgtccgtg gcccccggta caggcacgcc aattgctgga ggcatgacct
     4021 atcaccaggc cgccagcgtt atccgaaaac tcaccgacgt gaactttgtc gccatggatg
     4081 tcgtcgaagt gtcgccgccc tatgatcatg ccgacatcac ctccattgct ggcgcgtcac
     4141 tcgtcatgga atatctctgc ctcaaggcct ggcagcgcgg ggcacgtgcg acgcccatgc
     4201 cggaatgaag ctcgttcccg agctgccaga acaccggccc gctgttctcg aactcaatcg
     4261 cctcgctttc aaaggcgacg aggagccccg gctgatcaag aatctggcgc gtggcaatag
     4321 catttttgcc tccttcgtcg ctctcgacgg cgaagaagtt gttggccaca tcatgtttag
     4381 tcccctccat ctggtggtca atggagaacg catcgatgcc gttgccctgg ctcccatgtg
     4441 tgtgactccc ggcaggcaga aacaggggat agggacccag ctggtcgact atgggatcga
     4501 gcagatgaaa cgggcggggc aggccgcaat catcgtcctg ggtcatgtgg atttctatcc
     4561 ccgctttggc ttccggcacg accttgccag gaacctcagc tgcgccttca acaagtatga
     4621 tgcctttatg ggactggaat tgcgccctgg atccctcgac ggaaaatcgg ggacatgcat
     4681 atatcctcgc gcctttgatt gatccccacg gaagatgaaa tgaagcaccc ccttgatccc
     4741 ctatcggaat ccgaaattgc aaacgccgcc gccctcgtgc gcgggaaagg cggcttaagc
     4801 agccacgact ggtttgnnnn nnnnnngaga caattgccct gcatgagccg gaaaaggccg
     4861 aacttgcgca gggaaatccg ccgcgccttg cgtttgtctg ctgctacgat ccgcatacgg
     4921 gccagacatg ggatggcatc gccgatctcg aaagcggcct gctccggaac tggcggcatg
     4981 tcgccggcgc tcaggcccgc atcgtatccg atgaattcgt catgggcgga gaggtggcca
     5041 aggccgatcc ccgcgtcatt gccgccctcg cgctgcgagg catcaccgat ctcacgggag
     5101 ttctgatcga agcctggtcc gccggcaatt tcggcattgc cgaagagcag ggggagcgga
     5161 ttgcctattg ccactgtttc ctccgcaaca cggctggcga caatccctat gagcggccca
     5221 tcgccaatct ccatccggtt gttgacctgc gccgccgtat cgtcacgcgg gtcgatgatt
     5281 tcggcgtcgt tcccctgccg cctgacggga agccgctccg gcaggaaagc ctccgcaccg
     5341 atctcaagcc gttacagatc acccagccgg aaggcccgag cttcaccgtc gagggaaatt
     5401 tggtgcgctg gcagaaatgg cagatgcgcg tcggatttca tgtccgcgat ggcctgatcc
     5461 tccacatgat cggctatgag gatgaagggc gcctccgccc catcatgcac cgcgcctcca
     5521 tggctgaaat ggttgtgccc tacggcgatc cccgcggcgg cgcctaccgt cgcaatgctt
     5581 tcgatac
//
LOCUS       DAORVN010000027         5579 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_9783_length_5579_cov_3.744388, whole genome shotgun sequence.
ACCESSION   DAORVN010000027 DAORVN010000000
VERSION     DAORVN010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 5579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5579
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9783_length_5579_cov_3.744388"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1264
                     /locus_tag="PKV23_00995"
     CDS             <1..1264
                     /locus_tag="PKV23_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003576278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HQX84036.1"
                     /translation="LVQSLAMTKKAAALANLELGALDPKIADAIMAACDQIIAGEGHE
                     FFVVDMLQGGAGTSTNMNANEVIANMALRRIGKPRGDYASINPNDHVNLSQSTNDVYP
                     TAVRLTILRDCPSLLDSQIALKAAFLKKAAEFDHIIKVGRTQLMDAVPMRLGSEFRAF
                     GVTVGEDVDRLKEFANLLREVNLGGTAIGTGINTPGDYPATVIRHLSKVSGVDLVTAG
                     DLIEATSDMGAFVTFSGVLKRIAVKLSKICNDLRLLNSGPRAGFGEIRLPSVQAGSSI
                     MPGKVNPVVPEVVNQIAYQVIGNDLTVTLAAEAGQLQLNAMEPVIVFNILQSMRMLMR
                     GMVTLREKCVLGIEADEERCRELLDGSLVTVTALNPYIGYDEAARVAKIALKTRRTIR
                     DVALSEGVMTEAQLAEAFTTDNLLGVRR"
     gene            1358..2608
                     /locus_tag="PKV23_01000"
     CDS             1358..2608
                     /locus_tag="PKV23_01000"
                     /inference="COORDINATES: protein motif:HMM:NF013895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HQX84037.1"
                     /translation="MPSATLPAAAPASTLTEELGTLVRTLQAHGPDAAGRLLNGVSSS
                     VAFEALVRLNQGFVQDILATLGPARRQSILDAATPALAQQWQRNQDFAPDTIGRMMEP
                     AQSVLAPDMSVGEAIEALRKLLKNVMVTYGYVVDGTGKLLGLVTMRDLLFTSHDTALT
                     DIMLTDVFAFRAGMAQSEAMQLTLARHYPVYPVCADDGTLLGLVRGQTLFEAKAFEIT
                     AQAGSMVGVEREERISTPLWRAFRFRHPWLQLNLLTAFLAGAVVALFQGTIDQLVILA
                     AFLPVLAGQSGNTGCQALAVTLRGITLGEIKDGDGMKLVTKEGLLGALNGGPVGIVAG
                     VAMYVTAAGQQSPDALMLAIVVFLAMIGACTVSGICGALVPLTLKRYGADPATASSIF
                     LTTATDIFSMGLLLGLATLMVWAF"
     gene            complement(2745..2821)
                     /locus_tag="PKV23_01005"
     tRNA            complement(2745..2821)
                     /locus_tag="PKV23_01005"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2785..2787),aa:His,seq:gtg)
     gene            complement(2921..3400)
                     /gene="phaP"
                     /locus_tag="PKV23_01010"
     CDS             complement(2921..3400)
                     /gene="phaP"
                     /locus_tag="PKV23_01010"
                     /inference="COORDINATES: protein motif:HMM:NF020918.2"
                     /note="Members of this family are phasins (small proteins
                     associated with inclusions such as PHA granules). Note
                     that several different families of phasins have been named
                     PhaP despite very little sequence similarity to each
                     other; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01841 family phasin"
                     /protein_id="HQX84038.1"
                     /translation="MSKKSKFEANAEMPSEALAAAADAVEMPKDALEFAQKSVDQAQV
                     AYTKAKDLAQDNAQVFEAVTSAYKTGVVDFQKKAMEFTQKNMEQAFDFSRKLFSIREF
                     GEVVTLQQNFVKDQAEAFKAQASELNEIAMRLTTEAAQPLKSSFEKSVKDFSKSFAA"
     gene            3682..>5579
                     /locus_tag="PKV23_01015"
     CDS             3682..>5579
                     /locus_tag="PKV23_01015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HQX84039.1"
                     /translation="MTRTEAPTLEDLRTLASAAWLWDGARGRIVWANPAGVAYFGGES
                     VFDLIDRPFDLGEPGVEKITNLCKSLSRGQVETTDLHFPSSGRTVPLACRCSIHSLAD
                     GRAGLLVIAAGPSDAANDIPIEKLALAFNLLPAAAAFMGRDGSLHRLNAAALLLLGAE
                     QRASLSALVADSSLAEDLLARLASTGTVSDVRRLGTRIGQRDIRLTLQRLGASADPVA
                     FAMVLLDDITERRALERTISNSPQATKPQLAAAEADAFLQLGKVLGEAVVAQPPVPEK
                     PRRKPVLIPRAVTTAIDNLVHAVVICKGEQVLHANPKALEIFGYPTVDELINDDAVWS
                     YFSTLGRPVPVATLVIANGRDVSFSAQLSEVAWHSGPARQFILKAVATAKQSEEKPAP
                     PPPPIIAAPAEAPAIPAAKDVIPLPESVQNTQPVADDELRAILDTATDGIITLDREGK
                     IHTFSAGAEAIFGYRIAEVADKPFGDLLAPESRKVLRDYLSALQGPGLASVFNDGREV
                     TAIVKQGGSVPLFLTIGALQSLASRAAFCAVVRDITQWKRTESELRDAKEAAEATSRQ
                     KSEFLARISHELRTPLNAIMGFSEVMRLERFGEIKNDKYRGYVNDIHVSGGLLLSLQL
                     DGRLVPGLV"
BASE COUNT         1080 a         1682 c         1698 g         1119 t
ORIGIN      
        1 actggtgcaa tcccttgcca tgaccaagaa agcggcggcc cttgccaatc ttgagcttgg
       61 cgcgctggac cccaaaattg ccgatgccat catggcggcc tgcgaccaga tcattgcggg
      121 cgaaggccat gagtttttcg tggtcgacat gctgcagggg ggtgcgggca cttccaccaa
      181 catgaacgcc aatgaagtca ttgccaacat ggcgctgcgc cgcatcggca aaccgcgtgg
      241 ggattatgcc agcatcaatc ccaatgacca tgtcaatctc tcgcaatcca ccaatgatgt
      301 ttatcccacg gcggtgcgcc tcactatctt gcgcgattgc ccatcgctgc tggacagcca
      361 gattgcgctg aaggcggcgt ttctcaaaaa ggccgccgag ttcgaccaca tcatcaaggt
      421 cgggcgcacg caattgatgg atgcggtccc aatgcggctg ggctcggagt tccgggcctt
      481 tggcgtcacg gtcggtgagg acgttgaccg cttgaaggaa tttgccaatc tgctgcgcga
      541 ggtcaatctt ggcggcacgg ccattggcac ggggatcaat acgccagggg attatccggc
      601 gacggtcatc cgccatcttt cgaaggtatc gggcgtggac ctcgtcaccg ccggcgatct
      661 catcgaagcc acttccgaca tgggcgcctt tgtcaccttc tcgggcgtgc tgaagcgcat
      721 cgcagtcaag ctttccaaga tctgcaatga cctgcggctg ctgaacagcg ggccgcgggc
      781 gggctttggc gaaatccgcc tgccctcggt gcaggccggc tcctcgatca tgccgggcaa
      841 ggtcaatccg gtggtgccgg aagtggtcaa ccagatcgcc tatcaggtga tcggcaatga
      901 cctcacggtg acgcttgcgg cggaggccgg gcagttgcag ctcaatgcca tggaacccgt
      961 catcgttttc aacatcctgc aatccatgcg gatgctgatg cgcggcatgg tgaccttgcg
     1021 cgagaaatgc gtgctgggca tcgaggcaga cgaagagcgc tgccgcgagt tgcttgacgg
     1081 ctcactcgtc acggtaacgg cactcaatcc ctatatcggc tatgacgagg cggcacgggt
     1141 ggcgaagatc gcgctgaaga cgcggcgcac gatccgcgac gtagccttga gcgaaggcgt
     1201 gatgacggaa gcccagctgg cggaagcttt cacgacggac aacctgctgg gcgtgcgcag
     1261 ataaaccaaa ctgaattgca ccaattgctt cttctcaaag cccgtggtcc attgctatcc
     1321 cttgacgttc ctatctgatt tcaaaggact ttcccccatg ccgtctgcca cgctgcccgc
     1381 tgccgcccca gccagtacct tgactgagga gttgggcacg ctcgtccgaa ctctgcaggc
     1441 ccatggccca gacgcggccg gtcgcctgct caatggcgtg tcttcttcgg ttgccttcga
     1501 agcgctggtg cggctcaacc agggtttcgt acaagacatc ctggccactc ttgggccggc
     1561 gcggcgccag tccatactcg atgccgcaac gcctgcactg gcgcagcagt ggcagcgcaa
     1621 ccaggacttt gcgcccgaca caatcggccg catgatggag ccggcccaat cggtgctggc
     1681 gcccgacatg agcgtgggcg aggccatcga ggcgctgcgc aagctcctga aaaacgtgat
     1741 ggtcacctat ggctacgtgg tggacggcac cggcaaactg cttggcctgg tgacgatgcg
     1801 ggacctgttg ttcaccagcc atgacacagc gctcaccgac atcatgctga cggatgtctt
     1861 cgcttttcgc gccggcatgg cgcaatccga ggcgatgcag ctcactctgg cccggcatta
     1921 tccggtctac ccggtgtgcg ccgatgacgg cacgctgctg ggcctggtgc ggggccagac
     1981 gctgttcgag gccaaggctt ttgaaatcac ggcgcaggcc ggcagcatgg tcggcgtgga
     2041 acgggaagaa cgcatttcca cgcctctctg gcgcgccttc cgcttccgcc atccatggct
     2101 gcagctcaat ctgctgaccg cgtttctggc aggcgccgtg gtggcgctgt tccagggcac
     2161 cattgaccag cttgtgattc tggctgcgtt ccttccggtg ctggccgggc aatcgggaaa
     2221 caccggctgc caggcactgg ccgtgaccct gcgtggcatc acgctcggcg agatcaagga
     2281 cggcgacggc atgaagctgg tcaccaagga gggcctgctt ggcgccctga atggcgggcc
     2341 tgtcggcatc gttgcggggg ttgccatgta tgtgacagcc gcggggcagc agtcgccgga
     2401 tgcgctgatg cttgccatcg tggtgttcct tgcaatgatc ggggcctgca cggtgagcgg
     2461 aatctgcggc gccttggtgc cgctgacact caagcgctat ggcgccgatc cggccaccgc
     2521 gtcgagcatc ttccttacaa ccgccaccga cattttcagc atgggcctgt tgctggggct
     2581 ggcaacgctc atggtgtggg cgttctgaaa aaaatttaat gcgccggaag aggatcccgc
     2641 ccggcgcttg tatagacaag attcgacaac cataatatcc gaaggcgatt ttgtcacgag
     2701 aatccgggaa cgatttttgt ggcaggcttt tcgtatgagg ttcttggtac agctgggtgg
     2761 gctcgaacca ccgacctccg gatccacaat ccggcgctct aaccaactga gctacagctg
     2821 catcttcaag tgccgcgcaa actagcgatg tgtgcctgca aattcaagca aacaaaaagg
     2881 cccgcgacaa acgcgggcct ttcgaaaagg tcaaacaggc tcaagcagcg aacgacttgc
     2941 tgaagtcctt gacggatttt tcgaagctgc tcttcagcgg ctgggccgcc tcggtggtca
     3001 ggcgcatggc aatctcgttg agttcgctgg cctgggcctt gaaggcttca gcctgatcct
     3061 tgacgaagtt ctgctggagg gtaacaacct cgccgaattc ccggattgaa aacagcttgc
     3121 ggctgaagtc gaaagcctgc tccatgttct tctgggtaaa ttccatggcc ttcttctgga
     3181 agtccacaac gcctgtctta taggcggaag ttaccgcttc aaaaacctgc gcattgtctt
     3241 gggccagatc ttttgccttt gtataggcta cctgagcctg atcaacggac ttctgggcaa
     3301 attccagagc atcctttggc atctcgacag cgtcagcggc ggcagcgagg gcctcgctgg
     3361 gcatttcggc attggcttca aattttgatt tcttgctcat ggtcaatctc caaactggtt
     3421 agcgacacga tttgtcgctt gaaacccata taagaagaaa aatggtgcat tgcaacataa
     3481 ttctcgcaat gcaccattgt gtgtggaatc gataccctgc gttaaccagt tgtacatttt
     3541 tatgcccttt ggttaaccaa aggtggacgc aatctgaaaa aattgattta gttcgccagc
     3601 gggtgggcca actgcaaatg gccagccaca tgttcgtaat cgcgtgggga tcggccgggg
     3661 taaatcctgg ctgcgggtat tttgacaaga accgaggcgc caacccttga agatttgcgc
     3721 acccttgcca gcgccgcctg gctgtgggac ggggcgcggg ggcgcatcgt atgggcgaac
     3781 ccggcggggg tcgcgtattt tggcggtgaa tcagttttcg acctcatcga ccggccgttc
     3841 gacctgggcg agcccggggt cgagaagatc accaatctct gcaaaagcct gagccggggc
     3901 caggtcgaaa ccactgatct gcatttcccc tcttcgggcc gaacggtgcc gctcgcctgc
     3961 cgctgctcca ttcactccct ggccgacgga cgggccgggc tcctggtgat tgccgcaggc
     4021 cccagcgatg cggccaacga cattccgatc gaaaaactgg cgctggcctt taacctgctg
     4081 ccggcagccg cggctttcat gggacgggac ggctcgctgc accgcctgaa cgcggcggca
     4141 ctcctgcttc tgggggcgga gcagcgggca agcctcagcg ctttggtggc ggattcgtcg
     4201 cttgccgaag acttgctggc gcggcttgcc tcaaccggaa cggtcagtga tgtcaggcgc
     4261 ctgggcaccc ggatcggcca gcgtgatatc cggctcaccc tgcaaaggct tggcgcctcg
     4321 gcagacccgg tagccttcgc catggtgctg ctcgacgaca tcactgagcg ccgtgcactg
     4381 gaacggacga tttcaaacag cccgcaagcc accaaacccc agcttgccgc tgcggaggcg
     4441 gacgcgtttc tccagcttgg caaggtgctg ggtgaagcgg ttgtcgcgca accgcccgtg
     4501 ccggaaaaac cgaggcgcaa accggtgctc ataccgcggg ccgtcaccac cgcaatcgac
     4561 aatcttgtcc atgcggttgt gatctgcaag ggcgagcaag tgctgcatgc caatccgaag
     4621 gcgcttgaaa ttttcggcta cccgacggtc gatgagttga tcaatgacga cgcggtgtgg
     4681 agctattttt cgacgctcgg cagaccggtt ccggtggcca cgctggtcat tgcgaatggc
     4741 cgcgacgtca gcttctcagc gcaattgtcc gaggttgcct ggcacagcgg ccctgcccgc
     4801 cagtttatcc tgaaggcggt tgccacagcc aagcaaagcg aggagaagcc tgcgccgccg
     4861 ccaccgccca tcatcgcagc gcccgcagaa gcgccggcaa ttcccgccgc gaaagacgtc
     4921 atcccgctgc cggagagcgt tcaaaatacc cagccggtgg ccgacgacga actgcgggcc
     4981 attctcgata cggcgacgga cggaatcatt acgcttgacc gggaaggcaa gattcacacc
     5041 ttcagcgccg gggcggaagc gattttcggc taccgcatcg cggaagtagc ggacaagccc
     5101 tttggcgacc tgctggcgcc ggaaagccgg aaagtgctgc gcgattatct ttccgccctg
     5161 caggggccgg gacttgccag cgtgttcaac gacgggcgtg aagtgaccgc cattgtcaag
     5221 cagggcggct ccgttccgct gttccttacc atcggggcgc tgcaatccct ggcctcgcgc
     5281 gccgcgttct gcgccgtggt gcgcgacatc acgcagtgga aacggacgga atcggaactg
     5341 cgcgatgcca aggaggcggc cgaggccaca agccggcaga aatcggaatt cctcgcccgc
     5401 atcagccatg aattgcgcac gccgctcaac gccatcatgg gcttctcgga agtcatgcgg
     5461 ctggaacgct ttggcgagat caagaacgac aaataccgcg gctatgtgaa tgacattcac
     5521 gtcagcggcg gcctgctgct ctcgctgcag ctcgatggcc gtctcgtgcc gggactcgt
//
LOCUS       DAORVN010000028         5568 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_9820_length_5568_cov_3.701796, whole genome shotgun sequence.
ACCESSION   DAORVN010000028 DAORVN010000000
VERSION     DAORVN010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 5568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5568
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9820_length_5568_cov_3.701796"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..504
                     /locus_tag="PKV23_01020"
     CDS             <1..504
                     /locus_tag="PKV23_01020"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HQX84040.1"
                     /translation="PPRTVLGPGAIVPLRGIPHEVHATTARRGVITIDQDARRILVPG
                     DPRHLSRRLKDWLKAQAKADLTEASARYAKSMAVDFRRISVRDQKSRWGSCSAAGDLS
                     YNWRLILAPSHILDYVAAHEVAHRQHMNHGPKFWRLVLTHCPHASTAKHWFKSNGAEL
                     HRFTAHQ"
     gene            complement(498..2585)
                     /locus_tag="PKV23_01025"
     CDS             complement(498..2585)
                     /locus_tag="PKV23_01025"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HQX84041.1"
                     /translation="MGLFGGRKRHSASERREPQLFDRKKRRGAKDPERPPRRRRSFLG
                     WLFSFAFKSAVLGCIALAAMFGYVWFSLSQKGLLQIPELQPGIMLLAEDGTVLSEQGS
                     FFGDQVRVSELPDYVPNALIAIEDHRFRSHFGVDPLSLVRAAYENFTAGRVVQGGSTI
                     TQQLAKNLFLKPERTYSRKAQELVLALWLETKFSKDDILQLYLNRVYYGSGATGIEKA
                     AQVFYHKSAVDLNLTEAATLAGVLKAPTNYNPITRPDASAERAGLVIDAMVDAGFISQ
                     GEADQAINAPATVVASDYVPATQYVVDWVNEQLPLLVKNYDQSIIVETTIDPQLQLLA
                     ERSLRTHLNEEGRKLRVSQGAMVVMDTFGAVKAMVGGKSYKRSQYNRATKAMRQPGST
                     FKPFVFLAAMEKGYTPESVAIDQPIRIGSWQPENYNNKYLGRVTLRTAMANSLNSVAA
                     QLANNVGPRNVVAVAHRLGITSHLGTDASIALGTSEVTLLELTSAFTPFANGGYSVVP
                     FSVRRIVTRDGQIIYERSGDGLGQAMSNRNLGSMNSMMRAVVNEGTAKKAQIPDFDIA
                     GKTGTSQDYRDAWFVGYSSYLVGGVWLGNDDNSPTRTVTGGSMPAAIWKDVMQVAHAN
                     LSPAPLPGYIEEQPESADPYLVSQDEGMDYEEFETPRRKRGFFERLFGGSDEPRAEKR
                     VRRQSKNDNNSIY"
     gene            complement(2694..4199)
                     /locus_tag="PKV23_01030"
     CDS             complement(2694..4199)
                     /locus_tag="PKV23_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008127451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase family protein"
                     /protein_id="HQX84042.1"
                     /translation="MLRLNAVDNVVVAITTVEKGVLTSEDIVASERIGKGHKMAAVPI
                     KAGAAVKKFGQIIGFAKADIPAGAWIHEHNIGMGELSHDYAFAKGAIQTDILPLEKQE
                     TFQGFRRANGKVGTRNYIGVMTSVNCSATVAKFIAEEINRSGILKDYKNIDGVVALKM
                     DNGCVIDFRGAIFDVLKRTSWGYATNPNMGGVMMVGLGCEGFQIPRFKEAYGIVEGNT
                     FRTMTIQETGGTRKTVAAGVEAVKAMLPYVNDVKREKCYASEIILALQCGGSDGYSGI
                     TANPALGAAVDLLVRQGGTAILSETPEIYGAEHLLTRRAVSREVGEKLVDVIHWWEDY
                     TKRNLMEMNNNPSPGNKLGGLSTILEKSLGAAAKGGTTALREVYRYAEPVTAKGFVFM
                     DTPGYDPVSATGQVAGGANMLCFTTGRGSAYGCKPVPSIKLATNSDVYRRMIEDMDIN
                     CGDVIEGVSIADKGQEIFNKVLSVASGEKTKSEELGYGDNEFVPWQVGAVM"
     gene            4298..5032
                     /locus_tag="PKV23_01035"
     CDS             4298..5032
                     /locus_tag="PKV23_01035"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HQX84043.1"
                     /translation="MNNTAVSFDIPTPPRKGTRGGAGDHIYLVLREAIVSLELKPGQI
                     LDKQVFVERFGVSRAPIYDAFTRLQAEALVDVVPQRGTTVSLIKISDARENMFLRRAI
                     EVEAVRAVTRNISGNHVEALKQNLRYQSAAVASGDNVGFHRFDLEFHAVLFEALGFER
                     VKAVAETARLGLDRVRRLLNSRRRQEFTLQEHQVILDEIQQRNVNAAANAMGAHLDAV
                     MKELEAFAAANPELFADLQSKSDGQS"
     gene            5029..>5568
                     /locus_tag="PKV23_01040"
     CDS             5029..>5568
                     /locus_tag="PKV23_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004672006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HQX84044.1"
                     /translation="MKKNLFKAPSGASLSLTEMGMGTAPLGNLYRPLSEKDAQDTLAA
                     AWKAGIRYFDTAPFYGLGISENRLNPFFRGHKRSDFVVSTKVGRLLEVCKPAERAGIG
                     KFFDTPSRKEVFDYTYDGIMRSLDLSLERLGLDHVDILYVHDVDVFTHGSLEIRDAHV
                     KTLMASGYKALQKLRDEKVI"
BASE COUNT         1093 a         1696 c         1547 g         1232 t
ORIGIN      
        1 ccgccacgca ctgttctggg gcccggcgcc attgttccgc tgcgtggcat tccccatgag
       61 gtccacgcca ccacggcgcg ccgcggcgtc atcaccattg atcaggacgc ccgccgcata
      121 cttgtgcccg gcgatccacg ccatttgagc cgccgactga aggattggct gaaagcccag
      181 gccaaggccg atcttactga agcgtcagcc cgctatgcaa aatccatggc tgtcgatttc
      241 aggcgcatct cagtccgtga ccagaaaagc cgctggggct cctgttctgc cgccggcgat
      301 ctttcctaca attggcgtct gattctcgcc cccagccata tcctggatta tgtcgcggcc
      361 catgaagtgg ctcatcgcca gcacatgaat catggcccaa aattctggcg cctcgtcctg
      421 acccattgcc cgcacgcctc gaccgccaag cactggttca aatccaatgg tgcggagttg
      481 caccgcttca cggcgcatca atagatcgaa ttattgtcat tcttggactg gcgccttacc
      541 cttttctcgg cccgcggctc gtcgctgccg ccgaaaagac gctcaaaaaa tccccgcttg
      601 cgtcggggag tttcaaactc ctcgtagtcc atgccctcat cctgggatac cagatagggg
      661 tcagcacttt ccggttgttc ttcgatgtag ccgggcaggg gcgccggcga cagattggca
      721 tgggcgactt gcatgacgtc tttccagatg gccgccggca tcgagccgcc ggttacggtt
      781 ctggtcggcg aattgtcatc attgccaagc cagacgccgc ccaccagata gcttgaatag
      841 ccgacaaacc aggcatcccg gtaatcctgg ctggtgccgg tcttgcccgc aatgtcgaag
      901 tcgggaatct gcgccttctt ggccgttcct tcattcacca cggcccgcat catcgagttc
      961 atcgagccca ggttgcggtt tgacatggcc tggccaagcc catcgccact gcgttcgtaa
     1021 atgatctgcc cgtcgcgcgt cacgatgcgg cgcaccgaga agggcacaac actgtagccg
     1081 ccattggcaa agggcgtgaa agccgaggtg agttccagca gtgtgacttc cgaggtacca
     1141 agcgcgatgg aggcgtcagt gccgaggtgc gaggtgatgc caaggcgatg cgccaccgcc
     1201 accacattcc tcggtcccac attgttggcc agctgcgccg caaccgagtt gagcgaattg
     1261 gccatggccg tacgcagcgt cacccggccc aggtatttgt tgttgtaatt ctccggctgc
     1321 cagctgccaa tgcggatggg ttggtcgatg gccactgact ccggcgtata acccttttcc
     1381 atcgccgcaa ggaagacgaa cggtttgaaa gttgaacccg gctggcgcat tgccttggtg
     1441 gcgcggttat attggctgcg cttgtaggat ttgccgccaa ccatggcctt gaccgcgccg
     1501 aatgtgtcca tgaccaccat ggctccctgc gaaaccctga gcttccggcc ttcttcattc
     1561 aaatgcgtgc gcagcgatct ttcagcgagg agctgaagct gtggatcgat ggtggtttca
     1621 acgatgatgg actggtcata gttcttgacc agcagcggca gctgttcgtt gacccagtcc
     1681 accacatact gggttgccgg aacataatca gaagccacga cggttgccgg cgcattgatg
     1741 gcctggtccg cttcgccctg gctgatgaag ccggcatcga ccatggcatc gatcaccagg
     1801 ccggcgcgct ccgccgaagc gtccgggcgg gtgatcggat tgtagtttgt cggcgccttg
     1861 agcactccgg ccagcgtcgc cgcctctgtc aggttcaggt caacggcgga cttgtggtaa
     1921 aaaacctgcg ccgccttttc aatgcccgtg gcgcccgagc cataatagac gcggttcaga
     1981 tagagctgga ggatgtcatc cttggaaaat tttgtttcaa gccacagcgc cagaaccagt
     2041 tcctgcgcct tgcggctgta ggtgcgttcc ggtttcagaa acagattctt ggcaagctgc
     2101 tgggtgatgg tcgagccgcc ctggaccacg cgtcccgccg tgaagttctc gtaggccgcg
     2161 cgcaccaggc tcaagggatc aaccccgaag tgcgagcgga agcggtggtc ttcgatggcg
     2221 atgagcgcgt tgggcacata gtcgggaagt tccgatacgc gtacctggtc gccgaagaac
     2281 gagccctgtt ccgaaagcac cgtgccgtct tccgccagca gcatgatgcc gggctgcagt
     2341 tcgggtattt gcagtaaccc tttctggctc agcgagaacc agacatagcc aaacatggcc
     2401 gcaagggcga tgcagccgag gactgcggac ttgaaggcaa aggagaacag ccagccaagg
     2461 aacgaacgcc gccgccttgg cggacgctct ggatcttttg cgccgcggcg cttcttgcgg
     2521 tcaaaaagct gcggttcccg ccgttcactg gcggagtgcc tttttctgcc accgaatagt
     2581 cccatctttt cccttgcccc aataggctct atgcctttgt tggggcttat catttaaccc
     2641 ttggttaagg tcaggttggg cgcagaaaat ggtccaatga tgacagggct gattcacatc
     2701 accgcgccga cctgccaggg cacaaactcg ttgtcgccat agcccagttc ctcggacttc
     2761 gtcttttcgc cggaggcgac acttaggacc ttgttgaaga tttcctggcc cttgtcggca
     2821 atcgaaacgc cttcaatgac atccccgcaa ttgatgtcca tgtcctcgat catgcgcctg
     2881 tagacatcgc tgtttgttgc gagttttatg gaagggactg gcttgcagcc atatgccgag
     2941 ccgcgccccg tggtaaagca cagcatattg gcgccgcctg ccacctggcc cgtggccgat
     3001 accggatcat aaccaggcgt gtccataaag acgaagccct tggcagttac cggttcggca
     3061 taacggtaga cttcgcgcag cgcagtcgtg ccgcccttgg ccgccgcccc cagggatttt
     3121 tcaagaatcg tcgaaagccc gccgagcttg ttgccgggag aaggattatt gttcatttcc
     3181 atgaggttgc gcttggtgta atcctcccac cagtggatca cgtcgacgag tttttcaccc
     3241 acctcgcgcg acaccgcgcg gcgcgtgaga agatgttcgg ccccgtagat ttccggcgtt
     3301 tcggagagga tcgcggtgcc gccctggcgc accagtagat cgaccgcagc ccccagggcc
     3361 ggattggcgg taatcccgga ataaccgtcc gaaccaccgc attgcagcgc caggatgatt
     3421 tccgacgcgt agcatttttc gcgcttcaca tcattgacat aaggcagcat cgccttgact
     3481 gcttccaccc cggccgcaac cgtcttgcgg gttccgcccg tttcctggat ggtcatcgtg
     3541 cggaaggtgt ttccttccac gattccataa gcttccttga agcgcgggat ctggaagcct
     3601 tcacagccca ggcccaccat catgacgccg cccatgttcg ggtttgtggc ataaccccac
     3661 gaagtgcgct tcaagacatc gaagatggcg ccgcggaagt caatgacgca gccattgtcc
     3721 atcttgaggg ccaccacgcc gtcgatgttc ttgtagtctt tcaggatgcc ggaccggttg
     3781 atctcttcgg caatgaattt cgcaaccgtg gccgaacagt tcaccgaagt catgacgccg
     3841 atgtaattgc gcgttcccac cttgccgttg gcccgcctaa acccttgaaa agtttcttgt
     3901 ttttcaagag gcaggatgtc ggtctggatc gctcccttgg caaatgcgta gtcatgggaa
     3961 agctcgccca tgccgatatt atgttcgtgg atccaggcgc ctgccggaat atcggccttg
     4021 gcgaaaccta tgatttgtcc gaattttttg accgcagctc cggccttgat aggcacggcc
     4081 gccatcttgt ggcccttccc gatgcgctcg gaggcaacaa tatcctccga cgtcagcact
     4141 cctttttcca ccgtagtaat cgccacgacg acgttatcaa cagcattgag acgcaacatg
     4201 tgctgctttt gtgatggttt atcgagcatt tgacgacctc tgggatgcaa tttcacatag
     4261 aacttgcgtt gcctattatt catactaaca tgttagtatg aataatacag cggtgagttt
     4321 cgacatacct accccgcccc ggaagggcac ccgaggcggt gccggcgatc atatttattt
     4381 ggtgttgcgc gaagcgattg tgtccctgga actcaagccc ggccagattc tggacaagca
     4441 ggtcttcgtc gagcgcttcg gcgtctcccg cgcccccatc tatgatgcct tcaccaggct
     4501 gcaggccgaa gccctcgttg acgtggtccc gcagcgcgga acgacggtca gcctgatcaa
     4561 gatttccgat gcccgggaga acatgttcct gcgccgggcc attgaagtgg aagcggtgcg
     4621 ggcggtgacc cgcaatatct ccggcaatca tgtcgaagcc ctgaaacaaa acctgcgcta
     4681 tcaatcggct gccgtggcat ccggtgacaa cgtcggcttt caccgctttg acctggagtt
     4741 tcatgcggtc ctgtttgagg ctttgggctt cgagcgcgtc aaggccgttg ccgaaaccgc
     4801 gagacttggc ctcgaccggg tgcgccgcct gctcaattcg cggcgccgtc aggaatttac
     4861 cctgcaggag catcaggtca ttctcgatga aatccagcag cgcaatgtca acgccgccgc
     4921 caacgccatg ggcgcccacc ttgatgcggt gatgaaggaa cttgaagctt ttgccgcggc
     4981 aaatcccgaa ttatttgccg acctccaatc aaaatcagac ggacaatcat gaagaagaac
     5041 ctgttcaagg cgccctccgg tgcttccctt tcgctcaccg aaatgggcat gggcaccgcc
     5101 cccctcggta atctctatcg cccactttcc gaaaaagatg cgcaggacac tcttgcggcc
     5161 gcctggaaag ccggcatccg ctattttgat acggcgccgt tttatggcct gggcatctcg
     5221 gaaaaccgac tcaatccgtt tttccgcggt cacaagcgca gcgactttgt cgtttctacc
     5281 aaggtcggtc gcctgctcga agtgtgcaag cccgccgagc gggccggcat cggaaaattt
     5341 ttcgatacgc cctcgcgcaa ggaagtgttc gactacacct atgacggcat catgcgctcc
     5401 ctggatctgt cgcttgagcg cctgggcctc gaccatgtcg atattctcta tgtccatgac
     5461 gtcgatgtct tcacccacgg ctcgctggaa atccgcgatg cccatgtcaa aaccctgatg
     5521 gccagcggct acaaggcctt gcagaagctc cgcgacgaga aagtgatc
//
LOCUS       DAORVN010000029         5543 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_9899_length_5543_cov_3.738520, whole genome shotgun sequence.
ACCESSION   DAORVN010000029 DAORVN010000000
VERSION     DAORVN010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 5543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5543
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9899_length_5543_cov_3.738520"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..982)
                     /locus_tag="PKV23_01045"
     CDS             complement(<1..982)
                     /locus_tag="PKV23_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018097044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HQX84045.1"
                     /translation="MSVAEYFETMDYGPAPESDAEVRAWLATHKSSFGHFIGGKFVPG
                     AKHFDSTEPATGKFLARVAEGSNKDIDAAVSAARRAQGPWEKLGGHGRARHLYALARM
                     LQRHARLFAVLESLDNGKPIRETRDLDVPLAVRHFYHHAGWAQLQDSELAPYKAIGVV
                     GQIIPWNFPLLMLAWKVAPALAAGNTVVLKPAEFTPLTALLFAELAHKAGLPPGVLNV
                     VTGEGATGAALVEHAGVDKIAFTGSTEVGRLIRKATAGTGKSLTLELGGKSPFIVFDD
                     ADLDGAVEGVVDAIWFNQGQVCCAGSRLLVQEGVATEFIKRLKRRMGKLRV"
     gene            complement(986..1966)
                     /gene="deoC"
                     /locus_tag="PKV23_01050"
     CDS             complement(986..1966)
                     /gene="deoC"
                     /locus_tag="PKV23_01050"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014494075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HQX84046.1"
                     /translation="MAQTNILSMSAAARNTGVAYDLDWIEDMRINLSASERRVASLPG
                     RRSVKKDAQAAWLLKAVTCIDLTTLSGDDTASRVRRLCAKAKQPVREDILEKLGFAGR
                     NIHTGAICVYHRFVKTAVEALEGSGIPVAAVSTGFPAGLVPHDLKLKEIEASVKDGAQ
                     EIDIVITREHVLTGNWRALYDEMRDFRNACGEAHVKAILATGDIKTMRNVAKASMICM
                     MAGADFIKTSTGKEAVNATLLVTLTMLRMIRAYQERTGFKVGYKPAGGVSAAKDVLNY
                     QILMKEELGRKWLEPDLFRIGASSLLADIERQLEHHVTGRYSAFNRHPVG"
     gene            complement(2009..>3205)
                     /locus_tag="PKV23_01055"
     CDS             complement(2009..>3205)
                     /locus_tag="PKV23_01055"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQX84047.1"
                     /translation="PSHRPSVAVLPFENFSTDPEQAHFADAVVEEITATLSRIGDFLV
                     IARNSSFTYKGRSVDVRQVGRELNVRYVVEGSVRRVGERVRITAQLVETDSGRHIWAG
                     KAEGATSDLFDLQDRLAEMVAGAVYPSVRKAEIERARMKRPDNLEAYDLVMRALPHLW
                     AHRMHENPEAIALLDRSLKLDPNYGLAAALCAWARAQQIVYNWTDDVEGERRKGLALI
                     ETAARHIGDDATGLTALGTAVMLLEGNARRALGFVERAVMVDPNHAWAWMRRGFGLVY
                     TGKPEEGLKAFARAERLSPLDPFTFNMHIGIGLAHFAAGRYAEAIRYPQMVLDERPGL
                     TWPYRDLAAYKAQMGDMDGARDALEKFVYLRPPLSLASIADGLKFMEGPLLDRYIEGL
                     RLAGME"
     assembly_gap    3206..3215
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3216..3783)
                     /locus_tag="PKV23_01060"
     CDS             complement(<3216..3783)
                     /locus_tag="PKV23_01060"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HQX84048.1"
                     /translation="MAKNERRLVPIVSLDVEGYSRLTELDETATLAAVRHVFTERVEA
                     TIGDYGGSVFKTMGDGILAQFASAVAAVEWVSGLQQQLYQAPVNAADGKTLQLRAGIV
                     MADVVVKNDDLFGEGVNLTVRVQGCSPPGGMAISKWMHEYLTGKTDLTFVDIGSQRLK
                     NIGHTVRVFIWHPDASSQERFANNVLLLV"
     gene            complement(3862..4650)
                     /locus_tag="PKV23_01065"
     CDS             complement(3862..4650)
                     /locus_tag="PKV23_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010911865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HQX84049.1"
                     /translation="MNISESTELYHDQMIGMLERVWGDGWLSPGGPAEVGRLLEGMDL
                     KGKSVLDIGCGAGGVDVLLVGTHGAGYVTGIDVEDTVLAHARERAVKGGLAAQIGLVK
                     VVPGPLPFPPGTFDFVFSKDSIVHIPDKHALMRDVFRVLKPGGWFVASDWLIGHDHEP
                     SPEMKAYIASEGLDFGMASPLRYADAMRAAGFERVETRSRNAWYRDRAREELALMKGP
                     LYAEAAAKLGRDFVDHNIEIWANMLPVLESGEHCPTHLRAWKPD"
     gene            complement(4660..5403)
                     /locus_tag="PKV23_01070"
     CDS             complement(4660..5403)
                     /locus_tag="PKV23_01070"
                     /inference="COORDINATES: protein motif:HMM:NF013168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HQX84050.1"
                     /translation="MIIYPDLELRKGRLVNLARNRIDSPIIYDLDPIQAARDLAAQGA
                     EWLHVVDLDAVFNDGENTAIIKDIIRKVSCPVQVGGAIRSMEKVHSWMEAGAGRVVIA
                     TAAVKYPHLVKAAATAYPDSVVVSIDARGGHVAIEGWTETTTFTPLEFAHQFENTGLA
                     AIIFTDIDRDEDHPESSIAHTTELAAKIRVPVIASGVVKTLDDISTLSYLPNIAGVIT
                     SRALFGGAFSFSEAHAIAIAAHGPTAQLL"
BASE COUNT         1074 a         1806 c         1576 g         1077 t
ORIGIN      
        1 cgacccggag cttgcccatg cgccgcttca gccgcttgat gaattcggtg gccacgccct
       61 cctgcaccag aagccgtgac cccgcgcagc acacctggcc ctggttgaac cagatggcgt
      121 cgaccacacc ttccaccgcg ccatcgagat cggcatcgtc gaacacgata aatggcgatt
      181 tgccgccaag ttccagggtc agggattttc ccgtccccgc cgtcgccttg cggatcaggc
      241 ggcccacttc ggtggaaccc gtaaaggcaa tcttgtctac gcccgcatgt tcaactaggg
      301 cagcacccgt ggcaccttca cccgtcacca cattgagaac ccccggcggc aagcctgcct
      361 tgtgggcaag ctcggcaaac agcagggccg taagcggcgt gaactcggca ggtttcaaaa
      421 cgactgtatt tccggcagca agcgctggcg ctactttcca cgccagcatc agcaaaggga
      481 aattccacgg aatgatctgg ccgacaacgc caatggcctt gtagggggcc aattcgctgt
      541 cctgcaattg cgcccagccc gcgtggtgat aaaaatgccg caccgccagt ggcacgtcga
      601 ggtctcgcgt ctcgcggatc ggcttgccat tgtcgaggct ttccagaacc gcaaacagcc
      661 gcgcatggcg ctgcagcatg cgggccagcg cataaagatg gcgcgcccgt ccatggccgc
      721 caagtttttc ccacggtcct tgcgccctgc gcgccgccga aacggccgca tcgatatctt
      781 tatttgaacc ttcggcaacc ctggccagaa acttgcccgt agccggttca gtgctgtcga
      841 aatgtttggc gcctggtaca aattttccgc caatgaaatg gccaaagctc gacttgtgcg
      901 tggcaagcca ggcgcgcacc tccgcatccg attccggcgc cgggccatag tccatggttt
      961 cgaaatattc agcgacgctc aatttctacc ccacgggatg gcggttgaag gccgaatagc
     1021 ggccagtgac atggtgctca agctggcgtt cgatatcagc cagcaggctt gaggcgccaa
     1081 tgcgaaaaag atcaggctca agccatttgc ggcccagctc ttctttcatc aggatttgat
     1141 agttgagaac atccttggcc gccgatacgc cgcccgccgg cttgtagccc accttgaatc
     1201 ccgtgcgttc ctgataggcg cggatcatcc gcagcatggt cagcgtcacc agcagcgtgg
     1261 cattgacggc ttccttgccg gttgacgtct tgatgaagtc cgcccccgcc atcatgcaga
     1321 tcatcgaggc tttggccacg ttgcgcatgg ttttgatatc gcctgttgcc agaattgcct
     1381 tcacgtgggc ctcgccgcag gcattgcgga aatcccgcat ctcgtcataa agggcccgcc
     1441 agtttccggt gagcacatgc tcgcgggtga tgacgatgtc gatctcctgc gccccgtcct
     1501 tcaccgaagc ttcaatttcc ttgagcttca gatcatgcgg cacaagccct gccggaaaac
     1561 ctgttgaaac ggcggcgacc ggaatcccgc tgccctccag cgcttcaacc gcggttttca
     1621 caaagcgatg gtaaacgcag atggcgcccg tatgaatgtt gcggccggca aatcccagct
     1681 tttcaagaat gtcttcgcgc accggctgct tggccttggc gcaaagcctc cgcacccgcg
     1741 aggccgtgtc gtcaccgctc aatgtcgtca gatcgatgca ggtgacggcc ttcagaagcc
     1801 acgcggcctg cgcatctttc ttgacagaac ggcggccagg caggctcgcc acccggcgct
     1861 cggaggccga aaggttaatg cgcatgtcct cgatccagtc aaggtcatag gccacgcccg
     1921 tattgcgcgc ggcggctgac atggaaagga tgttggtctg ggccatagtg ccagttaggg
     1981 acgcgactgt ggcttagtca agtcgggcct attccatccc cgccaggcgc agtccctcga
     2041 tgtagcggtc cagcagcggg ccctccatga acttcagccc gtccgcgatg gaagcaaggc
     2101 tgagcggcgg gcgcaggtaa acaaacttct ccagcgcgtc ccgggcgccg tccatgtcgc
     2161 ccatctgcgc cttgtaggcc gcaaggtcgc ggtaaggcca ggtcagcccc ggccgttcat
     2221 caagaaccat ttgcggatag cggatggcct cggcatagcg gcccgccgcg aaatgcgcca
     2281 gcccgatgcc gatgtgcatg ttgaatgtaa agggatcgag tggcgaaagc cgttccgccc
     2341 gtgcaaaggc cttgaggccc tcttcgggtt ttcccgtata aaccaggcca aagccgcgcc
     2401 gcatccaggc ccaggcatgg ttcgggtcca ccatcacggc ccgctccacg aagccaagcg
     2461 cccgccgcgc atttccctca agcagcatga cggcggtgcc gagcgccgtg aggcccgtgg
     2521 cgtcatctcc aatgtggcgc gccgcggttt caatcagcgc caggcccttg cgccgttcgc
     2581 cttcaacatc gtcggtccag ttgtagacaa tctgctgggc ccgggcccag gcacacagcg
     2641 ccgccgcaag cccgtaattc ggatcaagct tgagcgaccg gtcaagcaat gcgattgcct
     2701 cgggattctc atgcatgcga tgggcccaga gatgcggcag ggcccgcatc acaagatcat
     2761 aggcttccag attgtccggg cgcttcatcc gggcccgctc gatctccgcc ttgcgcaccg
     2821 atggataaac tgcacctgca accatttccg caagccggtc ctgcaggtcg aacaggtcgg
     2881 acgttgctcc ctccgccttg ccggcccaga tatgacggcc tgaatccgtt tcaacgagct
     2941 gcgccgtgat gcggacccgt tcgccgacgc gccgcacgct gccttccacc acatagcgca
     3001 cattgagttc acgccccacc tgccgcacat cgaccgaacg gcccttgtag gtaaaggagg
     3061 aattgcgcgc gatgacaagg aaatcgccga tgcgcgacag ggtggcggtg atttcttcca
     3121 ccaccgcatc ggcaaaatgc gcctgctccg ggtccgtcga gaagttttca aacggcagta
     3181 cagcaaccga tggccgatgg ctgggnnnnn nnnnnggaca agtaaaagaa cgttgttggc
     3241 aaagcgctcc tgggaactgg catcgggatg ccagatgaaa acccgaaccg tatggccgat
     3301 atttttcagt cgttgcgagc cgatatcgac gaatgtcagg tccgtcttgc ccgtcaggta
     3361 ttcgtgcatc cacttggaga tcgccatgcc ccccggcggc gaacagccct gcacccggac
     3421 tgtcaggttg accccttcgc caaacaggtc atcgttcttg accaccacat cggccatgac
     3481 gattccggcc cgcaattgca gcgtcttgcc atcggcggcg ttgactggtg cttgatagag
     3541 ctgctgctgc aaccccgaaa cccactccac cgccgcaacc gcgctggcga actgggcaag
     3601 aatgccatcc cccattgtct tgaaaacact gccgccatag tcgccgatag tggcctcgac
     3661 gcgttccgtg aagacatggc ggactgccgc aagcgttgcc gtctcgtcaa gctcggtcag
     3721 ccgtgaataa ccctccacgt ccagcgagac aattggcaca agcctgcgtt cattcttggc
     3781 catgggggca cgtcgttccg atcttattgt cccctaatta ccacgctccg tcgggttcgc
     3841 aagttttccc tgaagtttgg cctaatccgg cttccaggca cgcaggtggg tggggcaatg
     3901 ctcgcccgat tccagcactg gcagcatatt cgcccagatc tcgatattgt ggtcgacgaa
     3961 atcccgcccc agcttggcgg cggcttccgc atagagaggg cccttcatca gggccagttc
     4021 ctcccgggcc cggtcccggt accaggcatt gcggctgcgc gtttcgaccc tttcaaaacc
     4081 ggcagcccgc atcgcatcgg catagcgcag cggcgatgcc atgccgaaat ccagaccctc
     4141 ggacgcaata taggccttca tctcggggct gggctcatgg tcgtggccga tcagccagtc
     4201 cgaggccaca aaccagccgc cgggcttcag cacccggaac acatcgcgca tcagcgcatg
     4261 cttgtccgga atatggacaa tcgaatcctt gctgaacacg aaatcgaagg tccccggcgg
     4321 aaatggcagg gggcccggca ccactttcac aagcccgatc tgcgcggcaa gccccccttt
     4381 cacggccctt tctcgggcat gggccagaac cgtatcctcg acatcgatgc ctgtcacata
     4441 gccggcacca tgcgttccca ccagcagcac atcaacgccg ccggccccgc agccgatatc
     4501 aaggacgctc ttgcccttga gatccatgcc ctcgagcagg cgccccactt ccgccggccc
     4561 gcccggcgag agccagccgt cgccccacac gcgctccagc atgccgatca tctggtcgtg
     4621 atagagttcc gtggactctg atatgttcat ggcacgatct tacagcagtt gcgcggttgg
     4681 cccgtgggcc gcgatggcta tggcgtgagc ttcgctgaag ctgaaagccc cgccaaacag
     4741 ggcccggctg gtgatcaccc ctgcgatatt gggcaggtac gacagggttg aaatgtcatc
     4801 cagggttttc accacgccgc tggcgatgac cggcacacgt atcttcgcgg caagctccgt
     4861 cgtatgggca atcgaacttt ccggatggtc ttcgtcgcgg tcaatgtcgg tgaagatgat
     4921 ggccgcaagc ccagtgtttt caaactggtg ggcaaattca agaggcgtga aggtcgtggt
     4981 ctcggtccag ccttcgatgg ccacatggcc gccgcgcgcg tcgatgctga cgaccactga
     5041 atccggatag gcggtggccg ccgctttgac gaggtgtgga tatttcaccg ccgctgtggc
     5101 gatcaccacc cggcctgctc ccgcttccat ccagctgtgg actttttcca tcgagcggat
     5161 ggccccgcct acctgcaccg ggcagctcac cttgcggatg atatccttga tgatggctgt
     5221 gttctcgcca tcgttgaaca ccgcatcgag atcgaccaca tgcagccatt ccgccccctg
     5281 cgccgcaagg tcgcgtgccg cctggatggg gtcgagatca tagatgatcg ggctgtcaat
     5341 cctgttgcgg gccaggttga ccaggcggcc cttgcgcagt tcaaggtcag gatagatgat
     5401 catcgtcgag ccctcccaag ttcaagatga gccttcagct tgcgccaaaa acccggtcgt
     5461 gaacaggggc cggtttagct tacgaagcgg gcaattcgag gcccgtcatc tcggcaatca
     5521 tccgcgccac cgaggcatcg tca
//
LOCUS       DAORVN010000030         5517 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_9983_length_5517_cov_3.459356, whole genome shotgun sequence.
ACCESSION   DAORVN010000030 DAORVN010000000
VERSION     DAORVN010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 5517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5517
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9983_length_5517_cov_3.459356"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            37..993
                     /gene="argC"
                     /locus_tag="PKV23_01075"
     CDS             37..993
                     /gene="argC"
                     /locus_tag="PKV23_01075"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012111862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HQX84051.1"
                     /translation="MTTIFIDGEAGTTGLQIRDRLKSRADIELVSIAQDKRKDAAARR
                     DLLNSVDVAILCLPDDAAREAVGLIDAKSRTRVIDASTAYRVDPAWAYGFPELSPAQA
                     KKIAGAARVSNPGCYPQGFIAVVKPLIDAGALSADALLTYNAVSGYSGGGKNMIEDYE
                     KAGAKASPYMPYGLTLNHKHLAEMKHYAGLSHDVIFQPVVGNYAQGMLGTVPLFLSML
                     KKKISVAAIHEILAKAYAASTFIEVAPVEAIERSPAIDPQALNNTNRMRLCVFGNEAR
                     GQVLLTSIYDNLGKGASGAAVQNLNLMIGAGESLGVDLPATN"
     gene            1006..1611
                     /gene="leuD"
                     /locus_tag="PKV23_01080"
     CDS             1006..1611
                     /gene="leuD"
                     /locus_tag="PKV23_01080"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019199127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HQX84052.1"
                     /translation="MEKFETLNGVAAPLNIVNIDTDMIIPKQFLKTIKRTGLGKSLFY
                     EMRYTQDGKEQPEFVLNKQAYRKAQILVAGDNFGCGSSREHAPWALLDFGIRCVISTS
                     FADIFYNNCFKNGILPIVVSPENLKKLLDDADRGSNATITIDLPRQEIRGPDGGTVKF
                     DIDPFRKHCLLNGLDDIALSLEKVSSIEAYEKKQAASHSWL"
     gene            1620..2567
                     /locus_tag="PKV23_01085"
     CDS             1620..2567
                     /locus_tag="PKV23_01085"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HQX84053.1"
                     /translation="MESILTTVLPVFGLIVLGYLFARLKLLDSATARGLTQFVFMIAI
                     PALLFRTIALMKPLDAMPFGLWLAFFGGLAATWILTTLISQKFDSLSASGGAAAAMAV
                     GFGNVALLGTPLGLAYYGEEAVIPLGLIISIHAPILWFAATLQRELARHSTAISYSRL
                     GRELFWSLAGNAIVLALAAAALWRLTGLGLNPVVDRMLAMLSEASIPTALFALGLSLA
                     TYSLRGQWSGMAVLIVMKMVLMPLLVFLALHYLVSVPPLWAKVAVLLAAMPTGANAFL
                     FAHRNEESVAAVSAAIALGTGLAAITVSLLLYWMDLGAL"
     gene            complement(2564..3595)
                     /locus_tag="PKV23_01090"
     CDS             complement(2564..3595)
                     /locus_tag="PKV23_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019965386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HQX84054.1"
                     /translation="MSKTDAGNFFEDFRLGQKIVHATPRTLGNGDSALYTALYGSRFA
                     VQSSDEFARSIGYPASPLDDFLVFNTVFGKTVPDISLNAIANLGYAECVFSTPVYAGD
                     TLSALSEVIGLKENSNGKTGTVYVRSKGINQKGEDVLSFVRWVMVRKRQEKSRPPAAI
                     VPLLQKTASVKAGMPRLNLSNWDFALSGSLQRFDDYAVGEKIDHLDGMTVEEAEHQLA
                     TRLYQNTARVHFNQFAEQGGRFGRRLVYGGHVISLARALSFNGLANAFRIAGINGGRH
                     VAPLFAGGTVFAWSEIIDKAKHGDAGALRIVTRATRDLACGDYPVEDGTAEKPGVILE
                     LDYWALIPR"
     gene            3676..4848
                     /locus_tag="PKV23_01095"
     CDS             3676..4848
                     /locus_tag="PKV23_01095"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008834475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HQX84055.1"
                     /translation="MEHVDIAIVGGGVVGSAAAYYLKKHGFSGSIAIIEKDTTYQFGC
                     TGRSLGGLRQQFSTPENIELSKFGLRLMRNLKQEFGPEADVSFHENGYLIMASQEGLP
                     ILEENHRIQLANGADNVILKGSDLTARFSWLVGDGVAGGCFGLSGEGWLDPYSLMTLL
                     RKSAVSQGVRIIPQELTAVNSAADRIASITLSNGNTLSCSHLVNAAGTGAGHVAGLAG
                     IELPVGPRKRYVYLLDCPQASEALHGAPLTVEPGGIYFRPEGRNFLCGLSPEEHEEPT
                     EMNWEVDYSWFEERIWPQLAERVPQFETIKVINAWVGQYDYNALDQNAIIGSHPRLGN
                     FHFANGFSGHGLQQGPAAGNAIAELIVHGTYRTIDLARLGYGRVAHNEPLFEKNVI"
     gene            4896..>5517
                     /locus_tag="PKV23_01100"
     CDS             4896..>5517
                     /locus_tag="PKV23_01100"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: protein motif:HMM:NF020132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HQX84056.1"
                     /translation="MGNSASVPALQAAGFAVSQIPTIILSNHPGLGKPSGIKLPPAEF
                     EAILKSLNALAVLDICVGVMTGYFASPDQVKIAASFIHKMKDKNPLLYVLVDPVLGDD
                     GSLYVSNEVATAIRDELLPLASCITPNCFELGWLTGKTVSSKAEVLRAAATLPHKEIL
                     ATSIPVGPGNLATLAITGSDYAETSSTVKVEVPHGTGDFLAGLYFAS"
BASE COUNT         1096 a         1768 c         1477 g         1176 t
ORIGIN      
        1 tgcactgatg caccatggcc tgtatgagac cccgccatga ccacgatatt catagatggc
       61 gaagccggca ccaccggcct gcaaatccgc gaccggctca aaagccgcgc cgatattgag
      121 cttgtcagca ttgcgcagga caagcgcaag gacgcggccg cgcgccgcga tctgctgaac
      181 agcgtcgatg tcgccatcct ctgcctcccc gatgacgccg cccgcgaagc ggtgggcctg
      241 atcgatgcca aatccaggac ccgcgtcatc gacgcctcga ccgcctaccg ggttgatccc
      301 gcctgggcct atggttttcc ggagttgagc cctgcccagg ccaaaaaaat cgccggtgcg
      361 gcccgcgtct caaaccccgg ctgctatccg caaggcttca tcgccgtggt gaagcctctc
      421 atcgacgcag gggcactgtc tgccgatgcg ctgctcacct acaatgccgt ttccggctat
      481 tccggcggcg gcaagaacat gatcgaggat tatgaaaagg caggtgccaa ggccagcccc
      541 tacatgccct atggcctgac gctaaaccac aagcatctcg ctgaaatgaa gcattacgcc
      601 ggcctcagcc atgacgtcat tttccagccc gtggtcggca attatgccca aggcatgctc
      661 ggcaccgtgc ccctcttcct ctccatgttg aagaagaaaa tcagtgtcgc cgccatccat
      721 gaaattctgg ccaaggccta tgccgccagc actttcattg aggtcgcccc cgttgaggcc
      781 atcgaacgct cccccgccat tgacccgcaa gcccttaaca acaccaaccg catgcggcta
      841 tgtgtttttg gcaatgaggc gcgcggccag gtgctgctaa cctcaatcta cgataatctc
      901 ggcaagggtg cctccggcgc cgccgtgcag aatttgaacc tgatgatcgg tgccggggaa
      961 agcctcggcg ttgatcttcc ggctacgaac tgaaggaaca ggatcatgga aaaatttgaa
     1021 accctgaatg gggttgccgc tcccctcaac atcgtcaaca tcgacaccga catgatcatc
     1081 cccaagcagt tcctgaaaac catcaagcgc accggtcttg gaaaatccct gttctatgaa
     1141 atgcgctaca cccaggacgg caaggaacag ccggagttcg tcctgaacaa gcaggcctat
     1201 cgcaaggcgc agatcctggt ggcgggcgac aatttcggct gcggctcgtc gcgcgagcac
     1261 gccccctggg cgctcctcga tttcggcatc cgctgcgtga tctcaaccag cttcgccgac
     1321 atcttctaca acaactgctt caagaacggc atcctgccca tcgtcgtgtc gccggagaac
     1381 ttgaagaagc tgctcgatga tgccgaccgc ggcagcaacg ccaccatcac catcgatctc
     1441 cccaggcagg aaatccgcgg gcccgacggc ggcacggtga aattcgatat cgatcccttc
     1501 cgcaagcatt gcctgctcaa cggcctcgat gacatcgccc tcagcctgga gaaggtgtcg
     1561 tcaattgagg cctatgaaaa gaagcaggca gcaagccatt cctggctctg acgcaagact
     1621 tggagtcgat cctcaccacc gtactccccg tctttggcct cattgtcctg ggttatctct
     1681 ttgcgcgcct gaagcttctg gatagcgcca ccgcccgcgg cctcacccag tttgtcttca
     1741 tgatcgccat tccggccctg ttgtttcgca ccattgccct gatgaaaccg ctggacgcca
     1801 tgccctttgg cctgtggctt gctttttttg gcgggcttgc cgccacctgg attctcacca
     1861 ccctcatttc gcagaaattc gacagcctct ctgcctcggg cggtgccgcg gctgccatgg
     1921 cggtcggctt cggcaatgtg gccctgctcg gaacgccgct gggccttgcc tattacggcg
     1981 aggaagccgt cattcccctc ggcctcatca tatccattca cgcccccatt ctctggttcg
     2041 cggccaccct gcagcgtgaa ttggcccgcc acagcacagc catttcctac agcaggcttg
     2101 gccgcgagct gttctggagc cttgccggca acgctatcgt gctggctctt gcggcggcag
     2161 cgctatggcg gctcaccggc cttggcctta accccgttgt tgatcgcatg ctcgccatgc
     2221 tctccgaggc cagcattccc accgcccttt ttgcacttgg cctgtcgctt gccacctatt
     2281 ccctgcgtgg ccagtggagc ggcatggccg tgctgatcgt catgaaaatg gtgctcatgc
     2341 cgcttctggt gttcctggcc ctgcattacc tggtatctgt gccgccgctc tgggccaaag
     2401 tcgccgtctt gctggccgcc atgccgaccg gcgccaacgc ctttctcttt gcccaccgca
     2461 atgaggaatc ggtggccgcc gtttccgccg caattgccct gggaaccggc cttgcggcca
     2521 ttaccgtgtc attgcttctg tactggatgg atttaggcgc gctctagcgc gggatcaggg
     2581 cccagtagtc cagttcgaga ataacgccgg gcttttccgc cgtgccgtct tcgaccggat
     2641 aatctccgca agccaagtca cgcgtcgccc gtgtcacgat ccgaagcgcg cccgcatctc
     2701 cgtgcttagc cttgtcgatg atttccgacc acgcgaacac cgttcccccg gcaaacagtg
     2761 gcgcaacatg gcggccgcca ttgatcccgg caatccggaa agcgttggca aggccattga
     2821 agctgagagc ccgcgccagt gaaatgacat ggccgccata aaccaggcgg cggccgaacc
     2881 tgccgccttg ctccgcaaac tggttgaaat gcacccgcgc cgtattctgg taaagccgcg
     2941 tggccaattg gtgttctgct tcctcaaccg tcatgccatc gagatggtcg atcttttcgc
     3001 ccaccgcata gtcgtcaaaa cgctgcaacg agccggacag ggcaaaatcc caattcgata
     3061 aattcaaacg cggcatgcca gccttgacgc tggcagtttt ttgaagcaac ggcacaatag
     3121 ccgccggcgg ccttgatttt tcctggcgct tgcgcaccat cacccagcgc acgaaactca
     3181 gaacgtcctc gcctttctgg ttaatccctt tgctccggac atagaccgtg ccggtttttc
     3241 cattggaatt ttcctttagg ccaatcacct cggaaagcgc cgacagcgta tcgcctgcat
     3301 agaccggcgt ggagaacacg cattccgcgt aacccaggtt ggcaatcgca ttgagcgata
     3361 tgtctggaac cgtcttgccg aacacggtgt tgaaaaccag gaaatcatca aggggcgagg
     3421 caggatagcc aatcgaccgg gcgaattcgt ccgaactctg aaccgcgaaa cgcgagccat
     3481 aaagggccgt atagagcgcg ctgtccccat tgcccagcgt gcgcggcgtt gcatggacta
     3541 ttttttgccc aagcctgaaa tcctcaaaga agtttcccgc atccgttttg ctcatcttca
     3601 ttgcatagcc attgtgccaa aataagtgaa gcccggcctt gtgagccggt tctggattgg
     3661 gcagaatgtt ttgtcatgga gcatgtcgat atagcgattg tgggcggcgg cgttgtcgga
     3721 agtgccgccg cctactatct caaaaaacac ggattttcgg gctctattgc gatcatcgag
     3781 aaagacacga cctaccagtt tggctgtacc ggaaggtctt tggggggctt gcgccagcag
     3841 ttttcgaccc cggaaaatat cgagctgtcc aaattcggcc tcaggctcat gcgcaacctc
     3901 aagcaggaat tcggtccgga agccgacgta agttttcatg agaacggcta tctcatcatg
     3961 gcttcgcagg aaggcctccc cattctcgag gaaaaccacc gtatccagct tgccaatggc
     4021 gctgacaacg tcatcctcaa gggcagcgat cttactgcac ggttttcctg gctggtgggc
     4081 gatggcgtgg cgggcggctg cttcggcctg tccggcgagg gctggctcga tccctattcc
     4141 ctgatgacgc tactgcgcaa gtcggcggtt tcgcagggcg tcaggatcat cccccaggag
     4201 ctcacggcgg taaacagcgc cgctgacagg atagcctcca tcaccctgtc aaatggcaac
     4261 accctctcct gctcccacct ggtgaatgcc gccggaaccg gcgctggcca cgtggcaggc
     4321 cttgccggca ttgagctgcc tgttggcccg cgcaagcgtt atgtctatct cctggattgc
     4381 ccccaggcca gcgaggcgct ccatggcgcg ccgctgactg ttgaacccgg cggcatttac
     4441 ttcaggccgg aaggccgcaa tttcctctgc ggcctctcgc cggaggaaca cgaagaaccg
     4501 acggagatga attgggaggt tgactattcc tggttcgagg agcgcatctg gccccagctt
     4561 gccgaacgcg tgccgcaatt cgaaaccatc aaggtgatca atgcctgggt cggccagtat
     4621 gattacaatg cgctggatca gaacgcgatt attgggtccc atccgcggct gggtaatttc
     4681 catttcgcca acggattttc cggccatggc ctgcagcagg gtccggcagc gggaaatgcc
     4741 atcgccgaac tcatcgtcca tggcacctat cgcaccattg atttggcgcg cctgggctac
     4801 ggccgcgtcg cccacaacga gcccctgttc gaaaaaaacg tcatctgatg ccacgccacg
     4861 tcctcagcat gtcctcgcaa gtggcttatg gacccgtggg aaattccgcc tccgttccgg
     4921 cccttcaggc tgcaggcttt gccgtatcgc agattccaac catcattctt tccaatcatc
     4981 ccggcctcgg caagccgtcc ggcataaaac tgccgcccgc ggaatttgag gccatcctga
     5041 aatcactcaa tgcccttgcc gttcttgata tctgcgtggg cgtgatgacc ggctatttcg
     5101 ccagccccga ccaggttaag attgccgctt ccttcattca taagatgaaa gacaaaaatc
     5161 cattgctcta cgtactggtc gatcccgttc tgggcgatga cggcagcctc tatgtctcaa
     5221 acgaggtggc caccgccatc cgcgacgagt tgctgccttt ggcctcgtgc attacgccaa
     5281 actgttttga actgggctgg ctcaccggca aaactgtctc cagcaaggca gaggtcttgc
     5341 gcgcggcggc gacgcttccg cacaaggaaa ttcttgcaac gtccattccc gtgggtcccg
     5401 gtaaccttgc aacccttgca atcaccggaa gcgactatgc ggaaacaagc agtactgtga
     5461 aggtcgaagt gcctcatggc accggcgatt ttctggcggg cctttacttt gcatccc
//
LOCUS       DAORVN010000031         5509 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_10010_length_5509_cov_3.157316, whole genome shotgun sequence.
ACCESSION   DAORVN010000031 DAORVN010000000
VERSION     DAORVN010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 5509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5509
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10010_length_5509_cov_3.157316"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2002)
                     /locus_tag="PKV23_01105"
     CDS             complement(<1..2002)
                     /locus_tag="PKV23_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013165342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HQX84057.1"
                     /translation="MTLPELCIRRPVFATVLSLILVLIGLMAFSRLTIREYPNIDEPQ
                     VSVSTGYNGASAEIIETQVTQVLEGSLAGIEGIDTIESTSRAESSRITIRFRPGISID
                     SATSDVRDRVSRVRRQLPDEIDEPNISKVEADAQAIIYLVMRSSGMTALELTDYVDRF
                     VVDRFKNLDGVADVSVNGERRYVMRVWLDPGKLAGYGVTVQDVEAAIRSQNAEIPAGR
                     VESTDREFTVLSRTSLGTAEEFARVILKPAGGLQVSLGDVARVELGTADVRRESRYDG
                     QTAISIGIVKQAVANPLDVAIAVKRVLPTINESMPEGTSIEVGFDSTVFIDRSINNVF
                     ITILEATGLVVIIIFLFLHSFRAAIIPVVTIPVSLLTAFAIMYATGLTINTLTLLAFV
                     LAIGLVVDDAIVMLENIYRHVEEGMKPFDAAIRGAREVGFAVIAMTMTLAAVYAPIAF
                     TPGRTGRLFMEFAVTLAGAVVVSGFVALTLTPMMSSKLLKHSERENFFSRTVERLLKS
                     LDRGYKTLLRQVLRLRWMVMLTALGIAGIGGLLLIQLKSELSPLEDRGVVMVSGNTPE
                     GSSFAYTQRYAEQVEELFQDIPDLRSALLIVGAGEVTRMISFARLKDWSERTISQQEI
                     VQTLLPKLRAIAGIQASASNPASLGVRGFGKPMQFVIQSSASY"
     gene            complement(2010..3110)
                     /locus_tag="PKV23_01110"
     CDS             complement(2010..3110)
                     /locus_tag="PKV23_01110"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HQX84058.1"
                     /translation="MKKSAAALVLLLALGGGAFFKQTSDQLKSLLPGASLAPTQTPAT
                     AGGGAKRAASSVEVATAEEGQLSDDIAAIGSLVSDESVDISAETNGRIVEILFADGTQ
                     VNAGDVLFRLDDTLVKAVVADAEARLALAKTVVERNAALRKSNNIAQSAVDQSNTELT
                     LAESALELAKVQLGKLTIRAPFSGRAGFRSVSIGAYVQAGAPLVHVEKIDVLKAAFSV
                     PELNYAQLSLGQNIMVTADAVPGETFEAKISAIDPLLDVNGRALRVRADLDNSADKLR
                     PGMLIRVSVRGPSRSSVMVPEAAIVPRSSGSVVFVATGEKAAETKVRTGKRVDGAVEI
                     IEGLKAGTQVVTAGNARLSDGASIAIVPPASN"
     gene            3298..4224
                     /locus_tag="PKV23_01115"
     CDS             3298..4224
                     /locus_tag="PKV23_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQX84059.1"
                     /translation="MSSAISISGLSKTYKSGFQALKDISLEIQKGEIFALLGPNGAGK
                     TTLISIICGIVNASAGKILANGHDIVTDFRAARSLIGLVPQELTTDAFETVWATVNFS
                     RGLFGLSPNPAHIEKTLKALSLWDKKDSKIMQLSGGMKRRVLIAKALSHEPQILFLDE
                     PTAGVDVELRRDMWQLVRDLRTTGVTIILTTHYIDEAEEMADRIGVINKGEIILVENK
                     KALMAKLGKKRIELHLHSALMAIPRKLSRYGLELNGDGTELTYTYDTQASRTGITSLL
                     EDLKAAKITFRDIETKQSSLEEIFVSLVRKPS"
     gene            4221..4982
                     /locus_tag="PKV23_01120"
     CDS             4221..4982
                     /locus_tag="PKV23_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009796844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQX84060.1"
                     /translation="MNLNAIRAIYVFEMARFFRTLTQSLVSPVISTSLYFVVFGAAIG
                     SRIQEIDGIDYGSFIVPGLIMLSLLTQSIANASFGIYFPRFVGTIFELLSAPVSYLEI
                     VIAYVGAAATKSIFLGLVILATAAFFVPLQILHPVWMLLFLVLTAVTFSLFGFIIGIW
                     ADGFEKLQLIPLLVITPLTFLGGSFYSISMLPPFWQKISLFNPVVYLVSGFRWSFFGH
                     SDVGIGYSLLAIAGFLAACLAVIAWIFRTGYRLKN"
     gene            complement(4979..>5509)
                     /locus_tag="PKV23_01125"
     CDS             complement(4979..>5509)
                     /locus_tag="PKV23_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015927744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid lyase"
                     /protein_id="HQX84061.1"
                     /translation="FAADMIAIAIAEMGSLSERRMAALIDSSISLLPPFLIRNPGLNS
                     GFMIPQCTAAALMSENKQMSHPASVDSAPTSANQEDHVSMATHGARRLGPMNANLARI
                     VAIELMASAEGIDFRRPLRSSKPLEEAHRLVRTKADAREQDREFSTDIEAIAGMIEQG
                     AFQPLVPKLLSDLGCA"
BASE COUNT         1108 a         1749 c         1511 g         1141 t
ORIGIN      
        1 cataggaggc ggacgattgg atgacgaact gcatgggctt gccaaagccg cgcactccta
       61 gggaggcagg atttgaggcg gacgcctgga tgccggcgat ggcccgcagc ttcggcagca
      121 gggtctgcac gatttcctgc tggctgatcg tgcgttcact ccagtccttg aggcgcgcaa
      181 aagaaatcat ccgcgtcacc tctcccgcac ccacaatcaa aagtgctgag cgcaggtcag
      241 gtatatcctg aaacagttct tccacctgct ccgcatagcg ctgcgtatag gcaaagcttg
      301 atccttccgg cgtatttccc gaaaccatga caacgccccg gtcttccaag ggcgagagtt
      361 cggatttgag ctggattagc agcaggccgc caatgcctgc gatgccaagg gcggtgagca
      421 tgaccatcca gcgcagccgc agcacctggc gcagcaacgt cttgtagccg cggtcgaggc
      481 ttttcagaag tctctcgacg gtccgggaga aaaagttctc ccgctccgag tgcttgagca
      541 gcttcgaaga catcatcggc gtcagcgtca gggcgacaaa gcccgacacc accacggcgc
      601 ccgccagcgt caccgcgaac tccatgaaaa ggcgccccgt gcgccccggc gtaaaggcga
      661 tgggagcata gaccgcggcc agcgtcatcg tcatggcaat cacggcaaag cctacttccc
      721 gggcgcccct tatggccgcg tcgaaaggct tcatgccctc ttccacatgg cggtagatgt
      781 tttcgagcat cacgatggcg tcatccacga cgaggccgat ggccagcacg aaagccagca
      841 gcgtcagggt gttgatcgtg agccccgtcg catacatgat ggcaaaggcc gtgagcagcg
      901 agacagggat ggtgaccacc gggatgatcg ccgcccggaa cgaatgaaga aacaggaata
      961 tgatgatcac cacgaggccg gtcgcctcaa gaatggtgat gaacacattg ttgattgaac
     1021 ggtcgataaa cactgtggaa tcaaagccca cctcgattga ggtgccctcc ggcattgact
     1081 cattgatcgt tggcagcacg cgtttgactg cgattgccac gtcaagcgga ttggcgaccg
     1141 cctgcttcac gatgccgatc gagatggccg tctgcccgtc ataacggctc tcgcgccgga
     1201 catcggccgt tccaagttca acccgggcca catctcccag cgaaacctgc aggccccccg
     1261 ccggcttgag gataactctt gcaaattcct ccgccgtgcc aagcgaggtg cgcgacagca
     1321 cggtgaattc gcggtccgtg ctttccaccc ggcccgccgg gatttcggca ttctgcgagc
     1381 ggatcgccgc ttcgacatcc tgcaccgtga cgccgtagcc cgcgagtttt cccggatcaa
     1441 gccagacccg catgacatag cgccgctcgc cgttcaccga aacgtctgcc acgccgtcga
     1501 gattcttgaa ccggtcgacc acgaaacggt cgacataatc cgtaagttca agggccgtca
     1561 tgccgcttga gcgcatcacc agatagatga tggcctgcgc atccgcctca accttcgaaa
     1621 tgttgggctc gtcgatttca tcgggaagct ggcggcgcac ccggctcacc cggtcgcgca
     1681 cgtcgctggt ggctgaatcg atgctgatcc cgggccggaa ccggatggtg atccggcttg
     1741 attccgcccg cgaggttgat tcaatcgtat cgatgccctc gattcccgca agcgagcctt
     1801 ccagcacttg cgttacctgg gtttcaatga tttccgctga agcgccgtta tacccggtgg
     1861 atacggaaac ctgcggttcg tcgatattgg gatattcgcg gatcgtcagg cggctgaagg
     1921 ccatgaggcc aatcagcacg aggatcaggc tcagcaccgt ggcaaaaacc ggtcttcgga
     1981 tgcagagctc tggaagtgtc attgccgcct caattgctgg ccggcgggac gatggcgatg
     2041 gatgccccgt ccgacagccg tgcgtttccc gcggtcacca cctgggtgcc ggccttcagt
     2101 ccttcaatga tttcgacagc gccatctacg cgctttccgg tgcgcacctt tgtttccgcc
     2161 gccttttcac ccgtggcgac aaaaaccaca gagccgctgc tgcgtggaac aatggccgct
     2221 tcaggcacca tcaccgaaga ccgcgaaggc ccgcggacac tgacccgtat cagcatgccc
     2281 ggccgcagct tgtcggcgga attgtcaagg tccgcccgca cccgcagagc ccggccattg
     2341 acgtccagca gcggatcaat cgccgagatt tttgcctcga aagtttctcc cggcaccgca
     2401 tcggcggtca ccatgatgtt ctggccaagg gacagctggg cgtaattcag ctccggcaca
     2461 ctgaaggccg ctttcagcac atcgattttc tccacatgca ccagcggcgc gcccgcctgc
     2521 acataggcgc ctatggaaac gctgcggaat cccgcccgcc cgctgaaggg ggcgcggatc
     2581 gtcagtttgc cgagttgcac cttggcaagc tcaagggcgc tttcggcaag cgtgagttcg
     2641 gtattggact ggtcgacggc gctctgcgca atgttgttgg acttgcgcag ggccgcattg
     2701 cgctcaacga cggttttggc cagcgcaagc cgcgcttccg catcggccac gacggccttg
     2761 accagcgtat cgtcaagccg gaacaacacg tcacccgcgt tcacctgcgt gccgtcggca
     2821 aacaggatct ccacgatccg tccatttgtc tcggccgaaa tatcgactga ttcatccgag
     2881 acaagcgagc cgattgccgc gatatcgtcg ctgagctggc cttcttctgc cgttgccact
     2941 tccaccgagg aagccgcgcg ctttgctccg ccgcctgccg tggcaggagt ctgcgttggc
     3001 gcaagcgagg ccccgggcaa aagggatttg agctggtcgc ttgtctgctt aaagaaggcc
     3061 ccgccaccga gagccaggag tagaaccaat gctgccgcag atttcttcaa gtaacgatca
     3121 ctccccgagt accatgccgc ctgcagcata acctactcaa gacggataag gcccggattt
     3181 ccgttcaaca ttcacgaatt tgtgtatccc aaaacccgtc gccgcaccaa acaaattgca
     3241 ggctggcact cttatcagca ccttgccttt ccagctttca gcccctattt ggacgcaatg
     3301 tcatccgcca tttccatctc cggcctttcc aagacctaca agtcaggctt tcaggccctg
     3361 aaagacatca gtcttgaaat ccagaagggc gagattttcg cccttttggg gcctaatggc
     3421 gccggcaaga ccaccctcat cagcatcatc tgcggcatcg tcaatgcaag tgccggcaag
     3481 atcctggcca acggccacga cattgtcacg gatttccgcg ccgcccgctc cctcatcggc
     3541 ctcgtgcccc aagaactcac caccgatgcc tttgaaaccg tctgggcaac cgttaatttc
     3601 agccgtggcc tcttcggtct gtcgcccaac ccggcccata tcgagaaaac cctcaaggcg
     3661 ctttccctct gggacaagaa agacagcaag atcatgcagc tttccggcgg catgaagcgc
     3721 cgggtgctga tcgccaaggc cctgtcccac gagccgcaga tcctgttcct cgatgagccc
     3781 accgccggcg tggacgtgga gcttcgccgc gacatgtggc agctcgtgcg cgatctcaga
     3841 acaaccggcg tcaccatcat cctcaccacc cactacattg acgaggccga ggaaatggcc
     3901 gaccgcatcg gcgtcatcaa caagggcgag atcatcctgg tcgagaacaa gaaggcgctg
     3961 atggccaagc tcggcaagaa gcgcattgaa ttgcacctgc acagcgcgct gatggccatc
     4021 cctcggaagc tctccaggta cgggctggaa ctcaacggcg atggcactga gctcacctat
     4081 acctatgata cacaggccag caggacgggc atcacctccc tgcttgaaga tctgaaagcg
     4141 gcaaaaatca ctttcaggga catcgaaacc aagcaaagct cgcttgagga aatattcgtg
     4201 agcctggtga ggaagccgtc atgaatttga atgccataag ggccatctat gtcttcgaaa
     4261 tggcgcgttt cttccgcacc ctcacccaga gccttgtctc gccggtgatc tccacctcgc
     4321 tctatttcgt ggtttttggc gccgccatcg gctcgcgcat ccaggaaatc gacggcatcg
     4381 actatggctc cttcattgtt cccggcctga tcatgctgtc gctgctcacc cagagcattg
     4441 ccaatgcctc gttcggcatt tatttcccgc gctttgtcgg caccatattt gaattgctct
     4501 cggcccccgt atcgtatctt gaaattgtca tcgcctatgt cggtgctgcg gcgaccaagt
     4561 ccatcttctt gggcctcgtc attttggcga ccgccgcctt ttttgtcccc ctgcagatcc
     4621 tgcaccctgt ctggatgctg ctcttccttg tgctgaccgc agtgaccttc agccttttcg
     4681 gcttcatcat cggaatctgg gctgatggat ttgagaagtt gcagctcatc cccctgctgg
     4741 tcatcacccc tctcaccttt ctcggcggca gtttctattc catcagcatg cttccgccct
     4801 tctggcagaa gatcagtctg ttcaatcccg tggtctatct cgtcagcggc ttcagatgga
     4861 gcttcttcgg ccattccgat gtcggcattg gttacagcct gctggccatt gctggttttc
     4921 tcgccgcctg ccttgctgtc atcgcctgga tcttccggac cggataccgc ctgaagaatt
     4981 aggcgcagcc gagatcgctg agcagtttgg gcacgagcgg ttgaaacgcg ccctgttcga
     5041 tcatcccggc aatagcctcg atatccgttg aaaattcccg gtcctgttcg cgggcgtctg
     5101 ctttggtacg caccagcctg tgcgcctctt ccagcggctt ggaagatctg agcggccgcc
     5161 ggaaatcaat gccctccgcc gaggccatga gttcgatggc aacgatccgc gccagattgg
     5221 cattcatcgg cccaagcctg cgcgccccgt gtgttgccat ggaaacatgg tcttcctggt
     5281 tggccgaggt gggggctgaa tcaaccgaag ccggatgcga catctgcttg ttctcgctca
     5341 tcagcgccgc cgccgtgcac tgcgggatca tgaaaccgga attgaggccc ggattgcgga
     5401 tgaggaacgg tggcaagagc gagattgagg aatcaatcag cgccgccatc cgccgttccg
     5461 aaagcgaccc catttccgca atggcaattg caatcatgtc ggcagcaaa
//
LOCUS       DAORVN010000032         5475 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_10105_length_5475_cov_3.097601, whole genome shotgun sequence.
ACCESSION   DAORVN010000032 DAORVN010000000
VERSION     DAORVN010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 5475)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5475)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5475
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10105_length_5475_cov_3.097601"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..790)
                     /locus_tag="PKV23_01130"
     CDS             complement(<1..790)
                     /locus_tag="PKV23_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017270164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HQX84062.1"
                     /translation="MNTQDSSAAPDELRDDAEGMSYLSHLPRRIVVIYLPLAIFVFVL
                     LFPFYWMAITAVKPNSQLTDYENYSPFWVVGATLEHINYLLFQTSYPGWLWNTILVSV
                     ASTFLSLFASVFAAYAIERVRFTGARTTGLLIFLAYLVPPSILFIPLAFIVFNIGIYD
                     SRFALIFTYPTFLIPFCTWLLMGYFRSIPYELEESALVDGATRWQILRRIILPLAVPG
                     LISAGIFAFTLSWNEFIYALTFIQSSENKTIPVGVLTELVRGDIF"
     gene            complement(801..1745)
                     /locus_tag="PKV23_01135"
     CDS             complement(801..1745)
                     /locus_tag="PKV23_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003526375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HQX84063.1"
                     /translation="MASRSSPGAISRSGSLLKRFNNSRNALGFVFMLPAAIFLLGFLT
                     YPLGLGVWLGFTDTRIGRAGDFVGLQNYFYLMDDQVFWLSVFNTVVYTLVASILKFAL
                     GLWLALILNEKLPFKSFFRAIVLLPWVVPTVLSALAFWWIYDAQFSILSWSLMKLGLI
                     DQPINFLGDPFNARVSVITANVWRGIPFVAISLLAGLQTIPVSLQEAASLDGATPWKR
                     FRYVTLPMLTPIIAVVMTFSVLFTFTDFQLIYVLTKGGPVNATHLMATLSFQRGIPGG
                     QLGEGAAIAVAMIPFLLASILFSFFGLQRRKWQQGGAD"
     gene            complement(1819..3129)
                     /locus_tag="PKV23_01140"
     CDS             complement(1819..3129)
                     /locus_tag="PKV23_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015888092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HQX84064.1"
                     /translation="MKINRRDLLAASAGIAGAAGFGLPGIRPAFAQEPAFAPEAGASL
                     RLLRWAPFVQGDEDAWLANTKKFTEATGVEVRVDKESWEDIRPKAAVTANVGSGPDMV
                     LCWFDDAHQYPDKLVDLTDLGTYLDGKYGGFYDGLKGYAMRGDKYIALPLAAIGNAIC
                     YRQSHIEAAGFKEFPKDTAGFLDLCKAMKAKGTPAGFAHGKAVGDGNNYVHWLLWSHG
                     GKMVDEAGVVAINSPETIAAINYAKALYDTFIPGTESWQDINNNRAFLAGQVSLTANG
                     VSLYYAAKKDEKLKDLTADLRATNFPMGPVGTNVELYQTTQAVVFNHTKFPNACKAYL
                     KFMYEAEQMNAWITGSSAYCCQPLKAFASNPVWTADPMHAPYAKASETLRPNGYAGPL
                     GYASAAVMADYVLVDMFAAAVTGAATPEEAAAQAEKRANRYYKV"
     gene            complement(3215..4276)
                     /gene="ugpC"
                     /locus_tag="PKV23_01145"
     CDS             complement(3215..4276)
                     /gene="ugpC"
                     /locus_tag="PKV23_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003526380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HQX84065.1"
                     /translation="MAPVDFINVRKSFGAVPIIKGVNIEIADGEFVILVGPSGCGKST
                     LLRMLAGLENITAGEIKIGGRVVNRMPPKERDIAMVFQNYALYPHMTVAENMGFSLLM
                     NGVAKQEIAAMVKPAAEILGLTNLLDRYPRQLSGGQRQRVAMGRAIVRNPQVFLFDEP
                     LSNLDAKLRVQMRVEIKELHQRLKTTTVYVTHDQIEAMTMADKIVVMHDGIVEQIGAP
                     LELYDRPVNLFVAGFIGSPAMNMIEGRLSPSDASVFLTTAGTALPLSRPMDAAKGKAL
                     IYGLRPEHVRLEANGIPATVVVTEPTGSETQVIVRLDQSDLTLMFHERIDVRPGETLH
                     LAIDKSAVHLFDKETGMRL"
     gene            complement(4800..>5475)
                     /locus_tag="PKV23_01150"
     CDS             complement(4800..>5475)
                     /locus_tag="PKV23_01150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX84066.1"
                     /translation="NNSRIYKFLPMHQVFAIAKDFLNSIGFRPGRRRAGKITTIAELG
                     DFIASRSAFVSQKTLYGYVQTRMGMNYAKNFQDEVFLASLNIAKMHVFAACLSDLAIY
                     AVAETTAGAHTSHETCRAIARQCFRNAIEENAGFAPTARWAPEAINEFEERLKDTIWE
                     FGALKPENFTRSPRALVRWSPIAPELKQFDTEIVENSIKFAWLEVREEFRRRVDRAAI
                     LAEAGP"
BASE COUNT         1139 a         1572 c         1682 g         1082 t
ORIGIN      
        1 caaaaatgtc gccacgcaca agttccgtga gaacgcccac gggaatcgtc ttgttttccg
       61 acgattggat gaaggtgagg gcatagatga attcgttcca ggacaatgtg aaggcgaaaa
      121 ttcccgccga aatgagcccc ggcaccgcca gcggcaggat gatgcggcgc aggatctgcc
      181 agcgggtggc cccgtccacc agggcgcttt cctcgagttc ataggggatc gaacggaagt
      241 accccatcag cagccaggtg cagaacggta tgaggaatgt gggataggtg aagatcaacg
      301 caaagcgcga gtcatagatg ccgatgttga acacgatgaa ggccagcgga atgaacagga
      361 tcgagggtgg caccagatag gcaaggaaga tcaggagccc cgtggtccgc gccccggtaa
      421 agcgcacgcg ctcgatggca taggccgcaa ataccgaggc aaacagcgag aggaaagtgg
      481 aggccaccga aaccagaatg gtgttccaca gccatcccgg ataggacgtc tggaaaagca
      541 gataatttat gtgttccagg gtcgcgccga caacccagaa cgggctgtag ttctcatagt
      601 ccgtcagttg cgagtttggt tttaccgccg tgatcgccat ccagtaaaac ggaaagagca
      661 ggacgaacac gaagatcgcc agcggcaggt agatcaccac aatccgccgt ggcaggtggc
      721 tgagatagct catgccctcc gcatcgtcgc gaagttcgtc tggtgccgcg ctgctgtcct
      781 gtgtgttcat ggcgccctca tcagtccgca ccgccctgct gccacttccg gcgctgcagc
      841 ccgaagaagc tgaacaggat cgaggcaagc aggaagggaa tcatggcgac ggcgattgcc
      901 gcaccttcgc ccagctggcc gccgggaatg ccgcgctgga aagacaatgt ggccatcaga
      961 tgggtggcgt tcaccggccc gccctttgtg agcacataga tgagctggaa atcggtgaag
     1021 gtgaaaagca ccgagaaggt catgaccacg gcgatgatcg gcgtcagcat cggcagcgtc
     1081 acatagcgga agcgcttcca gggcgtggcg ccatccagcg aggcggcctc ctgcagcgac
     1141 accgggatgg tctgcaggcc cgccagcagc gagatggcaa caaagggaat gccgcgccag
     1201 acattggctg ttatgacgga aacccgggcg ttgaagggat cgccgagaaa atttatcggc
     1261 tggtcgatca ggcccagctt catcagggac caggacagga tcgaaaactg cgcatcgtag
     1321 atccaccaga aggccagcgc cgacagcacc gtgggaacca cccacggcaa cagcacgatg
     1381 gcccggaaga aggatttgaa aggcagcttt tcgttgagga tgagcgccag ccaaaggccc
     1441 agggcgaatt tcaggatgga agcgacgagc gtgtagacaa ccgtgttgaa aactgaaagc
     1501 cagaacacct ggtcatccat gaggtaaaaa taattctgca ggccgacaaa atcgcccgcc
     1561 cggccgatcc gcgtatcggt gaagccgagc cagacaccga gccccagcgg ataggtgaga
     1621 aagcccagca ggaaaatcgc cgcaggcagc atgaacacga aaccaagcgc gttccggctg
     1681 ttgttgaagc gcttcaggag tgaacctgag cggctgatcg cacctgggct agatcgactg
     1741 gccatgcagt ggtctcttca cggaaaactt ttaaaggagt gcgggccacc cgccttgtca
     1801 gggatggccc gcattgtttt agaccttgta gtagcggttg gcgcgcttct cggcctgtgc
     1861 cgcggcctct tccggcgtgg cggcgccggt tacagcggcc gcgaacatgt cgaccagcac
     1921 gtagtccgcc atgacggcgg cggatgcata gcccaagggg ccggcatatc cgttcgggcg
     1981 cagggtttcc gaagccttgg catagggcgc atgcatcgga tccgccgtcc acacggggtt
     2041 ggacgcaaag gccttcagcg gctggcagca ataggcgctg gagccggtga tccaggcgtt
     2101 catctgttcg gcctcataca tgaatttgag gtaggccttg caggcgttcg ggaacttcgt
     2161 gtggttgaag acgaccgctt gcgtcgtctg atacaactcc acgttcgtgc cgaccggccc
     2221 catggggaag ttcgtggcgc gaaggtcagc cgtcaagtcc ttcagcttct cgtccttttt
     2281 cgccgcgtaa tagagcgaaa cgccgtttgc cgtgagcgac acctggcccg cgaggaaggc
     2341 gcggttgttg ttgatgtcct gccagctttc cgtgcccgga atgaaggtgt cgtaaagcgc
     2401 cttggcatag ttgatggcgg cgatcgtctc cgggctgttg atggccacaa cgcctgcctc
     2461 atcgaccatt tttccgccgt ggctccacag cagccagtgc acatagttgt tgccgtcgcc
     2521 caccgccttg ccatgggcaa agccggccgg cgtgcccttg gccttcatcg ccttgcaaag
     2581 atcgaggaag cccgcagtgt ccttcgggaa ctccttgaag ccagcggctt cgatgtggct
     2641 ctggcgatag cagatggcat tgccgatggc ggccagcggc agcgcgatgt acttgtcacc
     2701 gcgcatcgca tagcccttca ggccatcata gaagccgccg tacttgccat cgaggtaagt
     2761 gccgaggtcg gtcaggtcca caagcttgtc gggatattgg tgggcgtcgt cgaaccagca
     2821 cagcaccatg tcggggcctg aaccgacgtt ggccgtcacc gccgccttcg gccggatgtc
     2881 ttcccagctt tccttgtcga cgcgaacctc gacgccggtc gcctcggtga atttcttggt
     2941 gttggcgagc caggcatctt catcgccctg cacgaaaggg gcccagcgca gaagccgcag
     3001 gctggcgccg gcttccggcg caaaggctgg ttcctgcgcg aaggcagggc gaatgccagg
     3061 caggccaaag cccgccgcac cggcaatacc ggccgaagcc gcaagtagat cacgtctgtt
     3121 gattttcatc atttcctcct gaacgtttcc gggatattcc aaattttccc tccggcgcca
     3181 tcccgtggcc gcaccggcgg ttcttagttt ccagtcagag ccgcatcccc gtttccttgt
     3241 cgaagaggtg gacggcggat ttgtcgatcg ccaggtgcag cgtttcgccc gggcgcacgt
     3301 cgatgcgctc gtggaacatc agcgtaaggt cggattggtc gagccgcacg atgacctggg
     3361 tttccgaacc ggtgggttcg gtgaccacaa ccgttgccgg aatgccgttc gcctccagcc
     3421 ggacatgctc cgggcgcagg ccatagatca gggccttgcc cttcgccgcg tccatcggcc
     3481 gtgacagcgg cagggcggtc ccggccgtcg taagaaacac gctcgcgtcg gatggcgaga
     3541 gccgtccctc gatcatgttc atcgccggtg agccaataaa gccggcgacg aagagattga
     3601 ccggcctgtc atagagttcc agcggcgcgc cgatctgctc gacgatgccg tcatgcatca
     3661 cgacgatctt gtcggccatg gtcatggcct cgatctggtc atgcgtcaca tagaccgtcg
     3721 tcgtcttgag gcgctggtgc agttccttga tctccacgcg catctggacg cgcagcttgg
     3781 catcaagatt cgacagcggc tcgtcgaaca ggaacacctg cggattgcgc acgatggcgc
     3841 ggcccatggc gacccgctgg cgctggccgc cggagagttg ccgcggatag cggtcgagca
     3901 gatttgtaag gccaaggatt tcggctgccg gcttcaccat ggcagcgatc tcctgcttgg
     3961 caacgccgtt catcaacagc gaaaagccca tattctcggc caccgtcatg tgcggataga
     4021 gcgcgtagtt ctggaatacc atggcgatgt cgcgctcttt cggcggcatc ctgttgacca
     4081 cccgcccgcc gatttttatt tctccggcgg tgatgttctc aagtccggcc agcatgcgca
     4141 gcaaggttga cttgccgcag ccggaagggc ccacgaggat cacgaactcg ccgtcggcga
     4201 tctcgatgtt gacgcccttg atgatgggga cggcgccaaa ggatttccgg acgttgatga
     4261 aatcgaccgg cgccatacac atcctcccag accaacgtgc cgaatgataa cgaacgggcc
     4321 tcgtctcggg cccttgatat ttttcgttat cctatacagg tgagcggcag tgtacccagt
     4381 cgagtcgatt agtcaaacgt ttggtggcat gcattgatcc aaattgtgct gtcagtcaaa
     4441 tcagttgcaa atagttgcag ttcaagtcgg cacgagaatc gcatctttct cttcttgcaa
     4501 tgcaacatga aacgggaaag cagtttgtgc ccatgagtcc aaagtagcat atcggctcgc
     4561 ggctccacga tgataggaaa ttggtagcgc ccggattcta tctgccatcg ggaaaccttg
     4621 gctttatgaa ttcaggtgcg gtgttggagg ggatggtcca tgcagctgtt ggccgcaatg
     4681 actggcaagg gaacttgggc tccatgttct ggggctgctc gccatggcaa tgcatggaaa
     4741 gttttcccgt gctcaaaagc gcgtgccgct gatttcacag gacttgccct gcgcccaggt
     4801 caaggccccg cttctgccag gatcgctgct cggtcaaccc gccgccggaa ttcttcgcgc
     4861 acttcgagcc aggcgaactt gatggagttt tccacaattt ccgtatcaaa ttgcttgagc
     4921 tccggggcaa tcggcgacca gcggacaagg gcccgcgggc tgcgcgtgaa attttccggc
     4981 ttgagggcgc caaattccca gatggtgtct ttcagccgct cttcgaattc gttgatggcc
     5041 tccggtgccc agcgggcggt tggcgcaaac ccggcatttt cttcaatcgc gttgcgaaag
     5101 cattggcgcg cgattgcgcg gcaggtctca tgactggtgt gcgcgccagc agttgtttcg
     5161 gctaccgcgt agatcgcgag gtccgacagg caggccgcaa acacatgcat tttggcgatg
     5221 ttaagcgacg ccaggaaaac ttcgtcctgg aagttcttgg cgtagttcat tcccatgcgc
     5281 gtctggacat agccgtaaag cgtcttttgc gaaacgaacg ccgaccggct ggcaatgaag
     5341 tcgccgagtt cagcgatcgt ggtgattttt cccgcgcgcc ggcggccggg ccgaaagcca
     5401 atgctgttga gaaaatcctt ggctatggca aaaacctggt gcatgggaag aaacttgtaa
     5461 atccgggagt tgttc
//
LOCUS       DAORVN010000033         5465 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_10138_length_5465_cov_2.929575, whole genome shotgun sequence.
ACCESSION   DAORVN010000033 DAORVN010000000
VERSION     DAORVN010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 5465)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5465)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5465
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10138_length_5465_cov_2.929575"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..464)
                     /locus_tag="PKV23_01155"
     CDS             complement(<1..464)
                     /locus_tag="PKV23_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006022982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HQX84067.1"
                     /translation="MSGAPRHFLDLSDFEGADLRIILNDAKAMKAGRAGLSKGTHETT
                     ALLAGKIIALIFQRPSTRTRVSFEVGIRQLGGESLLISAGDMQLGRGETIADTAKVLS
                     RFVDGIMIRAQNHDDLMELAQHATVPVINGLTNKSHPCQIMADILTLEEHVGP"
     gene            complement(464..1642)
                     /locus_tag="PKV23_01160"
     CDS             complement(464..1642)
                     /locus_tag="PKV23_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HQX84068.1"
                     /translation="MIAAVLPTYNRAKISFERGEGMRVFTPEGDAYLDFGGGVAVTSC
                     GHAHPHLVKALTDQAQKIWHTSNIYQMPGQEKLAQRLIDATFADTVFFTNSGAEALEC
                     AIKMARKYHAAKGHPERYRLITFEGAFHGRTLATIAAGGQAKYLEGFGPKVEGFDQVP
                     FGDFEALKAAIGPETAGILIEPIQGEGGVRPVPPEDLRAMRALCDEHGILLVLDEVQC
                     GIGRTGKLFAHEWAGITPDIMAVAKGIGGGFPLGACLATENAAQGMTAGTHGSTYGGN
                     PLATAVGNAVLDVVLEKGFLDNVLQRSLGLKQKLARLKDEHPNVIEDIRGSGLMMGIK
                     CKVPNTEFQAKAMEHKLLTIAAGDNVIRLLPPLIATDSDITEAVNKLEMTCRSLEVKK
                     "
     gene            2039..2566
                     /locus_tag="PKV23_01165"
     CDS             2039..2566
                     /locus_tag="PKV23_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009208717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GcrA family cell cycle regulator"
                     /protein_id="HQX84069.1"
                     /translation="MPWTDERVELLKKLWTEGHSASQIAARLGMGVTRNAVIGKVHRL
                     NLSGRVTQPRSTEPRAPRKTREPSHPGRPVGSPSMPSTGNTALKTFVRVEPVQRQIAL
                     PEPQPLRLVDLPKGGRVTILHLSDKTCKWPIGDPGSEDFCFCGHSPRDKSPYCEYHAR
                     AAYQPMQDRRHRKFG"
     gene            complement(2637..3335)
                     /gene="phoB"
                     /locus_tag="PKV23_01170"
     CDS             complement(2637..3335)
                     /gene="phoB"
                     /locus_tag="PKV23_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011475316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon transcriptional regulator
                     PhoB"
                     /protein_id="HQX84070.1"
                     /translation="MSASLLIVEDEEPIQILLSYNFEAEGYRVRATAQGEDVARLVNE
                     ERPDLIVLDWMLPGISGIEVCRLLRTRPETRDIPIIMLTARGEENERVRGLATGADDY
                     LVKPFSVPELLARVRTILRRANPDAVAEVLKAGDIALDRRARRVTRSKRDIELSPTEF
                     RLLEHLMQNPGRVYSRSQLLDAVWGRDIYVDERTVDVHVGRLRKSLQRGRESDPIRTV
                     RSMGYAFDERFGAA"
     gene            complement(3346..4056)
                     /gene="phoU"
                     /locus_tag="PKV23_01175"
     CDS             complement(3346..4056)
                     /gene="phoU"
                     /locus_tag="PKV23_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HQX84071.1"
                     /translation="MSDHTVKSYDDDLASLKQMLAQMGGLAEEQLANAIDAIAKRDTR
                     LADIVIAGDEKIDALEQQIEEKSILTIAKRQPMAQDLRQIIVAIRISADLERIGDLAK
                     NTAKRTHAISEQMPRKLVSGLTRMGNLAQEQLKNILDAYARNDADKAISVWRSDEDID
                     ALYNSIFRELLTYMMEDPRMIGPCTHLLFGTKNMERIGDHTTNIAENIYFLVHGKAII
                     EERPKKDSTSTTNYNLIK"
     gene            complement(4076..4873)
                     /gene="pstB"
                     /locus_tag="PKV23_01180"
     CDS             complement(4076..4873)
                     /gene="pstB"
                     /locus_tag="PKV23_01180"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008549528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HQX84072.1"
                     /translation="MPTLSKVPRILNERETKIVASDVNVYYGEKQAIKNLSIDIPDRA
                     VSAFIGPSGCGKSTFLRTINRMNDTIDGCRVTGDILVDGKNIYDRDLDVVQLRARVGM
                     VFQKPNPFPKSIFENVAYGPRIHGIAKSKADLEEIVVNSLKKASLFEEVKDRLDHSGT
                     SLSGGQQQRLCIARAIAVNPEIILMDEPCSALDPIASARIEELIDEIRQNYCIIIVTH
                     SMQQAARVSQLTAFFHLGDLVEFGDTDEMFTTPKDKRTQDYITGRFG"
     gene            complement(4908..>5465)
                     /locus_tag="PKV23_01185"
     CDS             complement(4908..>5465)
                     /locus_tag="PKV23_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006473263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HQX84073.1"
                     /translation="WTEIIEVNINNLAAVPSIIFGLLGLAVFINFFGLPRSAPLVGGL
                     VLALLVLPTIIIASRAALKAVPPSIKEAALGVGASHQQAVFHHVLPLAVPGILTGTIL
                     GVARALGETAPLLMIGMVAFIVDVPKGFTEAATVLPVQIFLWSDLPEIAFQSRTAAAI
                     IVLLVILFGLNAAAIYMRKRFERRW"
BASE COUNT         1062 a         1633 c         1653 g         1117 t
ORIGIN      
        1 ggccccacat gctcttccag ggtcaggatg tcggccatga tctggcaggg atgggatttg
       61 ttggtgaggc cgttgattac cggaaccgtg gcgtgctgcg ccagttccat gaggtcatcg
      121 tggttctggg cccggatcat gatgccatcc acaaaacgcg atagaacctt cgccgtatcg
      181 gcgattgtct ctccccgacc cagttgcatg tcgcctgccg aaatgagcag cgattcaccg
      241 ccaagctgcc ggatgccgac ttcgaaggaa acccgggtgc gggttgaagg ccgctggaag
      301 atcagcgcaa tgattttccc ggcaagcagg gcggttgttt catgcgtgcc cttgctcagg
      361 cccgcgcgcc ccgccttcat cgctttcgcg tcattgagaa tgatgcgcaa atccgcgccc
      421 tcgaaatccg agaggtcgag aaaatgtctg ggcgctccgc tcatcacttc ttcacttcaa
      481 ggctgcggca ggtcatttca agcttgttga cggcctcggt gatgtcagag tcagtggcaa
      541 tcagcggcgg cagaaggcgg atgacattgt cgcctgccgc gatggtgaga agcttgtgct
      601 ccatggcctt ggcctggaat tccgtattgg gcaccttgca cttgatcccc atcatcaggc
      661 cggagccgcg gatgtcttca atgacgttgg gatgctcgtc cttgagccga gccagttttt
      721 gcttcaggcc gagcgagcgc tgcagcacat tatcgaggaa gcccttttcc agaaccacat
      781 cgagcaccgc attgcccacc gccgtggcca gcggattgcc gccataggtg gagccatggg
      841 ttcccgccgt catgccctgc gccgcgtttt cagttgcaag gcacgcaccc aatggaaagc
      901 cgccgccaat gcccttggcc accgccatga tatccggcgt gatgccggcc cattcatggg
      961 caaacagttt gcccgtgcgg ccgatgccgc actgcacttc gtcaaggacc agcagaatgc
     1021 catgttcatc gcaaagcgcc cgcatggccc gcaggtcttc cggcggcacc gggcgaacgc
     1081 cgccctcacc ctgaatgggt tcgatcagga tgcctgcggt ctccgggcca attgcggcct
     1141 tcaacgcttc aaaatcacca aagggaacct ggtcgaagcc ttcgaccttg ggcccgaagc
     1201 cttccagata ttttgcctgg ccgccggcgg caatcgtggc cagcgtgcgc ccgtgaaacg
     1261 ccccttcgaa tgtaatgagg cggtagcgct ccggatgccc cttcgccgca tgatacttcc
     1321 gcgccatctt gatcgcgcat tccagcgctt ccgccccgga gttggtgaag aacaccgtgt
     1381 cggcaaaagt tgcgtcgatc aaacgctggg ccagtttctc ctggcccggc atctggtaga
     1441 tgttcgaggt gtgccagatc ttctgcgcct gatctgtcaa cgccttgacc agatgtggat
     1501 gcgcatggcc gcagcttgtc accgcaacgc cgccgccaaa atccagatag gcgtctcctt
     1561 ccggcgtgaa aacccgcatg ccttccccgc gctcaaaact gattttggcg cgattgtagg
     1621 tcggaaggac ggctgcgatc atcggtttgg tcccgttgca aagcaaaatg cgccgctcgg
     1681 ggaacggcgc aagttatttt ttccagacta taagtccatg attgcgcacg gtcaatcgca
     1741 tcgcgtgcaa cgcactcatc aacatgagtc aaaacagcgc atggacctcc gattcccgat
     1801 ggcggtaagg tcttgttaac caagcggcgg cgcgtgatta gctggggaaa acataaaaaa
     1861 cactgtgccg gattcttgaa gtcgagtcac cgcgtatagt agtgtccgct cattcgagct
     1921 acgatatgta gcgtttcaaa gtcaattata gcgtcaacac gctgagaggt catttcagcg
     1981 ctagatgggg gattcaaaat tgaacgagat gccagcacaa gttatcggca acgaaaagat
     2041 gccctggacc gacgagcgtg tggagcttct caagaagctc tggaccgagg gccacagcgc
     2101 cagccagatc gccgcccgtc ttggcatggg ggtcacgcgc aacgccgtta tcggcaaggt
     2161 gcaccgcttg aatctgtcgg gccgtgtcac ccagccgcgc tccaccgagc cgcgcgcccc
     2221 gcgcaagacc cgcgagccca gccatcccgg caggccggtg ggctcaccct ccatgccgag
     2281 cactggaaac accgcgctca agacctttgt gcgggttgaa cccgttcagc gccagatcgc
     2341 gctgccggaa ccgcagccgc tcaggttggt ggatctgccc aagggcggcc gcgtcaccat
     2401 ccttcatctc tccgacaaga cctgcaagtg gccaatcggc gatccaggct cggaagattt
     2461 ctgcttctgc ggacactccc caagggataa gtcgccctac tgcgaatacc atgcgagagc
     2521 tgcctaccag ccgatgcagg accgccgtca ccggaagttc gggtaagtac gagacacttg
     2581 ccacttttta attctgtcat cccggcgaaa gccgggatga caggttgggg ggcgggctac
     2641 gccgcgccaa atctttcatc gaaggcatag cccatgctgc gcaccgtgcg gatggggtcg
     2701 ctttcgcggc cgcgctgcag ggatttccgc aagcggccca catgcacgtc aaccgtgcgt
     2761 tcatccacat agatgtcgcg gccccacacg gcatcgagca gctgcgagcg cgaatagacc
     2821 cggcccggat tctgcatcag gtgctcgagc aggcggaatt ccgtgggcga aagctcgatg
     2881 tcgcgcttgg agcgcgtcac ccgccgcgcc cgccggtcga gcgcgatgtc gcccgccttc
     2941 aacacctcgg ccaccgcatc gggattggcg cgccgcaaaa tggtgcgcac ccgcgccaga
     3001 agttccggca ccgagaatgg cttcaccaga taatcatctg ccccggtggc aagcccccgc
     3061 acccgctcgt tctcttcgcc gcgcgccgtg agcatgatga tgggaatatc gcgggtttcg
     3121 ggccgcgttc gcagcaggcg gcacacctcg atgccggaaa tgccaggcag catccagtcc
     3181 agcacgataa ggtcaggccg ctcttcgttc accagacggg ccacgtcctc gccctgcgcc
     3241 gtggcgcgca cccggtagcc ctcagcctcg aaattatagc tgagaagaat ctggatcggt
     3301 tcttcgtctt caacaatcag gagcgaagct gacattgtcc ccgtctcact tgatcaggtt
     3361 gtagttggtg gtgctggttg aatccttctt cgggcgttct tcgatgatcg ccttgccgtg
     3421 caccaggaaa tagatgttct cggcgatatt ggtggtgtgg tcgccgatgc gctccatatt
     3481 cttggtgcca aacagcaggt gggtgcaggg gccgatcatc cgcgggtctt ccatcatgta
     3541 ggtgagaagc tcgcggaata tggaattgta gagcgcatcg atgtcctcat ccgagcgcca
     3601 caccgagatg gccttgtcgg cgtcgttgcg ggcataggcg tcaaggatgt tcttaagctg
     3661 ctcctgggcc agattgccca tgcgggtgag ccccgacacc agtttgcgcg gcatttgctc
     3721 ggagatggcg tgggtgcgct tggccgtgtt cttggccagg tcgccgatgc gctcaaggtc
     3781 cgccgaaatc cggatggcga caatgatctg ccgcaggtcc tgtgccatgg gctggcgctt
     3841 ggcgatggtg agaatggatt tttcctcgat ctgctgttcg agcgcgtcga tcttttcatc
     3901 cccggcgatc acaatgtccg ccagcctggt atcgcgcttg gcaatggcat cgatggcatt
     3961 ggcaagctgc tcttccgcca gcccgcccat ctgcgccagc atctgcttga ggctggccag
     4021 atcgtcgtca taggatttaa ctgtgtggtc cgacatgaag gtctccgttc tgggctcagc
     4081 cgaagcggcc ggtgatgtag tcttgcgtgc gcttgtcctt gggcgtggtg aacatctcgt
     4141 ccgtgtcgcc aaattcaacc aggtcgccga ggtggaagaa ggccgtgagc tgtgaaaccc
     4201 gcgccgcctg ctgcatggaa tgcgtcacga tgatgatgca gtagttttgc cggatctcgt
     4261 caataagctc ctcaatgcgc gccgaggcaa tggggtcaag ggccgagcag ggctcgtcca
     4321 tcaggatgat ttccgggtta accgcaatgg cgcgtgcgat gcacaggcgc tgctgctgcc
     4381 cgcccgacag gctggtgccg gaatggtcga gccggtcctt cacctcttca aaaaggctgg
     4441 ccttcttcag gctgttgacg acgatttcct ccaggtcggc ctttgatttg gcaatgccat
     4501 ggatgcgggg gccataggcc acgttctcga atatggactt tggaaaaggg ttcggcttct
     4561 ggaacaccat gccgacccgg gcacggagct gcaccacgtc gaggtcgcgg tcatagatgt
     4621 tcttgccgtc gaccaggatg tcgcccgtca cccggcagcc gtcgatcgtg tcgttcatcc
     4681 ggttgatggt gcgcaggaag gtggacttgc cgcagcccga ggggccgatg aaggctgaaa
     4741 ccgcgcggtc cggaatgtcg atggaaagat tcttgatggc ctgcttctcg ccgtaataga
     4801 cattcacgtc gctggccacg atttttgtct cccgttcgtt gagaatgcgg ggaactttgg
     4861 aaagtgtggg catttggggc gaagcgtcca tgatgtttcc ttatgtctta ccagcgccgc
     4921 tcgaagcgct tgcgcatgta gatggcggcg gcattgaggc caaagagaat gacaagaagc
     4981 acgatgatgg cggcagccgt gcgcgattga aacgcgatct ccggcaagtc cgaccacagg
     5041 aagatttgaa ctggcaacac ggttgccgct tcggtaaacc ccttgggaac atcgacgatg
     5101 aaggccacca tgccgatcat cagaagcggt gccgtttcgc caagggctcg cgccacgccc
     5161 aaaatcgtgc cggtcaggat gccgggtacg gccagcggaa gcacatggtg gaatacggcc
     5221 tgctggtgcg aggcgccgac ccccagcgcc gcttccttga tggatggggg cacggctttc
     5281 agcgcggcgc gcgatgcgat gatgatggtt ggcaggacca ggagcgccag cacaagcccg
     5341 cccaccaggg gtgccgaacg cggcaggccg aagaaattga tgaagaccgc aagcccaagc
     5401 aggccaaaga tgatggaagg aacggcggcc agattgttga tgttgacctc gatgatctcc
     5461 gtcca
//
LOCUS       DAORVN010000034         5349 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_10516_length_5349_cov_3.137892, whole genome shotgun sequence.
ACCESSION   DAORVN010000034 DAORVN010000000
VERSION     DAORVN010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 5349)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5349)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5349
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10516_length_5349_cov_3.137892"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            118..339
                     /gene="infA"
                     /locus_tag="PKV23_01190"
     CDS             118..339
                     /gene="infA"
                     /locus_tag="PKV23_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018266249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HQX84074.1"
                     /translation="MAKEELLEFEGTVSELLPNATFRVLLDNNNHEIIAHTAGKMRKN
                     RIRVLAGDRVMVEMTPYDLTKGRINYRFK"
     gene            346..984
                     /locus_tag="PKV23_01195"
     CDS             346..984
                     /locus_tag="PKV23_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family nucleotide pyrophosphatase"
                     /protein_id="HQX84075.1"
                     /translation="MADTRVKLVLASASPRRLMLLERMGLAPDLLNPTDLDETPKRRE
                     TPRRLSIRLAEEKAKAAIHAPQVQALGAGVFILAADTVVGLGRRILPKAESRKDAYGC
                     LTLLSGRSHWVYSTVCIIAPNGQKSTRCSETKVRLKRLSREDIESYLKSDEWRGKAGG
                     YAIQGRAEAFVRQLTGSYSGVMGLPLYETVHLLEGAGYPVLHTWMTTASTVV"
     gene            984..1187
                     /gene="yacG"
                     /locus_tag="PKV23_01200"
     CDS             984..1187
                     /gene="yacG"
                     /locus_tag="PKV23_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014761011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HQX84076.1"
                     /translation="MAEPVRLKPRRPCPICGKPSLQKFHPFCSARCAQVDLNRWLGGN
                     YAIPAVENEEPSEGDLDNDRQVD"
     gene            1221..1859
                     /locus_tag="PKV23_01205"
     CDS             1221..1859
                     /locus_tag="PKV23_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018389118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HQX84077.1"
                     /translation="MADARILIVDDHPLFREALRNALGTAKTRLDISEAGSLDALISH
                     LARDDSCDLVLLDLRMPGVQGLSGLIYLRSQYPNIPVVIVSASEEPGIMRKSLHLGAS
                     GFIPKSSGAATIIEAVETVLAGGVWAPPWTESEPPADSEAATIAERVASLTAQQIRVL
                     LMLKEGLLNKQIAHALNVSEATVKAHVSAILLKLGVASRTQAVIAASKLEGL"
     gene            2111..2641
                     /locus_tag="PKV23_01210"
     CDS             2111..2641
                     /locus_tag="PKV23_01210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX84078.1"
                     /translation="MTPAASHAIAGTTNDLAEEIQPQYNLRFERVAAGPGRGFCLVDV
                     LLRNNGLIPANFPFFCLTVLGLNAAPAPGWAQQEITIVRKMQRYTPVTSSTLDPGALA
                     QCCTIKLRYKSAFGGSLEFEPGSEHLLTDFPNLNLTCVVGAGNFPSRRTLLQVPATAF
                     RMLLKNKDASQPAADI"
     gene            complement(2664..>5349)
                     /locus_tag="PKV23_01215"
     CDS             complement(2664..>5349)
                     /locus_tag="PKV23_01215"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain-containing hybrid sensor histidine
                     kinase/response regulator"
                     /protein_id="HQX84079.1"
                     /translation="AAPSDIRRAAWLFPLYLVAINIFVIPIAIAGLVMLPASADGDTF
                     VLALPVSAQSGFFAMVAFIGGLSASTAMVVVETIALSIMICNNLVVPLLLRQRTERPE
                     LLHDMGKTLITIRRLAIALVLVLAYGYYRMIGSTAALAQVGLISFAAVAQFAPAFFGA
                     LMWKRATARGAMAGLTAGALVWGYTLLLPSFADAGWMGKGLIEQGPFGLDFLRPRMLF
                     NMELDALSHGVLWSLAANICAYVGFSLSRLPTPIERIQAASFVTRELTVGTGTGFRLW
                     RTAVTVEKLEQTVARYLGEARARSAFAEFFAQRGVTQKPALEADVRMLRFAEHLLARA
                     VGVASSRLVMALLLERHSAHPRGAMRLLDDASAAIQHNRDLLQSAIDNVEQGIAVFDS
                     EMNLSWNASFHDHLKLSNELGRVGAPMREIVLEFLRTTESGEASLEDRLRKMAITHEH
                     FRERLCDSGQVLEIRSNAIPDGGVVVTFNDATESVRAAETLQKSNEELERRVVQRTVE
                     LTRLNDELAVAKAAAESANLGKTRFIAAASHDILQPLNAARLFTSSLVERLKRSNDGA
                     LARNVDTSLEAVEEILTALLDISRLDAGVMLPELSIFRIDDLMETLSTEYAPAASKKN
                     LVLKFMHCGATVETDRKLLRRVMQNFISNAIKYTRSGRILMGCRRKGRALRIEIHDTG
                     IGIPDSKRELVFQEFQRLGDDGGSATGLGLGLSIVERVARILGSEITLRTTPGKGSMF
                     AITVPLSHVVIPRVRTRSVSLQRPGLGITAAVLVIDDEPAILEGMKHLLSGWGCTPHL
                     ARGSAEALRAYDIADGRIDMILADFHLNKEDGISLINELRRRARRPIPAILITADRSP
                     AVADLATVHDIHVLRKPVRPAALRAAMSHLEIRAVAGE"
BASE COUNT         1071 a         1604 c         1664 g         1010 t
ORIGIN      
        1 gttcggagct ctgggggcgc ggggcgtata aaatatgggt tttgtcgcag tctcttgcat
       61 ttggcggcca agaggtccat ttatcgcgcc gcttcctttt aacaaccgga aagattcatg
      121 gcgaaagaag aacttctcga atttgagggc acggtatccg agcttctgcc caacgcgacg
      181 tttcgcgtgc tgctcgacaa taacaatcat gaaattattg cccacacggc gggcaagatg
      241 cgcaagaacc gtatccgggt tctcgctggt gaccgcgtga tggttgaaat gacgccctac
      301 gatctgacca agggccgcat caactaccgt ttcaagtaag tccatatggc tgatacccgc
      361 gttaagcttg ttctggccag tgcttcgccg cggcgtttga tgcttctgga gcgcatgggc
      421 ctggcgcccg acctgctcaa tcccacggat ctcgacgaaa cgccgaaacg gcgcgaaacg
      481 ccgcgccggc tttcaatcag gctggccgaa gaaaaggcca aggccgccat ccatgcgccg
      541 caagtgcagg cgctgggcgc aggggtgttc attctcgcgg ccgacacggt ggttgggctg
      601 gggcgccgga tccttcccaa agccgaatcc cgcaaggatg cctatggctg cctcaccctg
      661 ctttcgggcc gctcccactg ggtttattcc acggtttgca tcattgcccc caatgggcag
      721 aaatccacgc gctgcagcga aaccaaagtc aggctcaagc ggctgtcgcg ggaagacatc
      781 gagagctatc tcaagtccga tgaatggcgc ggcaaggcgg gcggctatgc catccagggc
      841 cgggcggaag ctttcgtgcg ccagcttacg ggctcctatt caggggtgat ggggctgccg
      901 ctttacgaaa ccgtgcattt gctggaaggt gctggctatc cggtgcttca cacctggatg
      961 acgactgctt ccactgtcgt gtgatggccg aaccggtccg cctcaagcca cgccgtccgt
     1021 gccccatttg cgggaaaccc tcgctgcaga aattccaccc cttctgttcg gcgcgctgcg
     1081 cccaggttga tctcaaccgc tggctgggcg gcaactatgc aatccccgcg gttgagaacg
     1141 aagagccttc cgagggtgat ttggacaacg accggcaagt ggactagcat cgggagatga
     1201 cgaggcaacg gggagcatga atggccgacg cacggattct gatcgtcgac gatcatcccc
     1261 ttttccgcga ggccctgcgc aatgcgcttg gcacggccaa gacccggctc gatatttccg
     1321 aggcgggctc gcttgatgcg ctgatttcgc atcttgccag ggacgacagc tgtgacctgg
     1381 ttctgcttga tttgcgcatg ccaggcgttc agggactctc gggcctgatc tacctgcgtt
     1441 cacaatatcc caatattcca gtggtcatcg tttcggccag cgaagaaccg ggcatcatgc
     1501 ggaagtcgct ccatctcggg gcttccgggt tcatcccgaa gtcatcgggc gcggcgacga
     1561 tcatcgaggc ggtggaaact gtcctggccg gtggtgtgtg ggccccgccc tggaccgaaa
     1621 gcgagccgcc cgctgattca gaagccgcga ccatcgctga gcgcgtagcc tcgctcacgg
     1681 cgcaacaaat ccgcgtcctg ctgatgctga aggaaggcct tctcaacaag cagatcgccc
     1741 acgctcttaa cgtttcggaa gcaacggtga aggcccatgt ctcggccatc ctgctgaagc
     1801 ttggagtggc cagccgcacc caggcggtga tcgccgccag caagctggaa ggattataac
     1861 cccgtaatag gctttaacac ttatagagag cgtgccacat cggatttccg gtctggcaag
     1921 ggttcgcttc ccttcgagat gaactcaaga ctgcgggacc ctcacgtagc attggggtta
     1981 attctgttaa gaagctttaa ttccagccct gactcccgat cagaaaatat tcacttaata
     2041 ttttaatcgg ccagtgatgc cttggaccta taaatgcagg ccagtttcag cttcatcaac
     2101 caggttccca atgaccccag cagccagcca cgcaattgca ggcaccacaa acgacctcgc
     2161 cgaagaaatt cagccgcagt acaatctgcg gttcgaacgc gtggctgcag gcccgggccg
     2221 gggcttctgc ctggttgacg tcctgctcag aaacaacggc ctcattcccg caaactttcc
     2281 cttcttctgt ctgaccgtcc ttggcctcaa cgcggccccg gcgccgggct gggcccagca
     2341 ggagatcacc attgtccgga aaatgcagcg ttacactccg gtcacctcga gcaccctcga
     2401 tcccggggcg ctggcccagt gctgcacgat caagctgcga tacaaatcgg cctttggcgg
     2461 cagcctggag tttgagccgg gaagcgaaca tctgctcacg gatttcccta acctgaacct
     2521 gacctgcgtg gtaggcgccg gcaatttccc gtcgcggcgg acactgctgc aagtgcctgc
     2581 aaccgcattc aggatgctgc tgaagaacaa ggatgcttcg cagccggcag ccgacatctg
     2641 acctgagcag cggaaccccc ggttcactcg ccggcaacag ccctgatttc aagatgcgac
     2701 atggcggcac gcagggcggc gggcctgacg ggcttgcgca ggacatgaat gtcatggacg
     2761 gtggcgaggt ccgccacggc cggagacctg tctgcggtaa tgagaatggc cggaatgggc
     2821 cggcgcgccc tgcggcgcaa ttcattgatc agtgaaatgc catcttcctt gttgagatgg
     2881 aaatcggcga ggatcatgtc gatcctgccg tcggcgatgt cataggcccg cagtgcctcg
     2941 gcgctaccgc gcgccaagtg cggcgtgcag ccccagccgc tgagcagatg cttcatgcct
     3001 tcgaggatcg ccggttcatc atcgatcacc agaaccgccg ccgttatgcc cagcccgggg
     3061 cgctggagcg acacgctcct ggtgcggacg cgcggtatga caacgtgcga caggggcacg
     3121 gtgatggcaa acatgctgcc cttgccggga gtggtgcgaa gcgttatttc gctgccgaga
     3181 atgcgcgcca cccgctcgac aatcgacagg ccaaggccaa ggcccgtggc tgaacccccg
     3241 tcatcgccca aacgctggaa ctcctggaat accaactccc gcttggagtc cggaatgccg
     3301 atgcccgtgt cgtgaatctc gatgcgcaac gcccgtccct tacgccggca gcccatcaga
     3361 atgcgtccgg atctggtgta tttgatcgcg tttgagatga agttctgcat gacacgccgc
     3421 aacagcttgc gatcggtttc aaccgttgcg ccacaatgca tgaacttgag aaccaggttc
     3481 ttcttggagg cggccggggc atattcggtg ctcagggttt ccatcagatc gtcgatccgg
     3541 aagatcgaca gttccggcag catgacgccc gcgtcaaggc gcgatatatc aagcaacgcc
     3601 gtcaaaattt cctccaccgc ctccagcgag gtatcaacat ttcgcgccag ggcaccatcg
     3661 ttggagcgct tcagcctttc caccaggctt gaggtaaaca ggcgggcggc attgagcggc
     3721 tgcaggatgt cgtggcttgc ggcagcaatg aacctggtct tgccgaggtt ggcagattcg
     3781 gctgccgcct tggccacggc cagttcatcg ttcagcctgg tcagttccac cgtgcgctgg
     3841 accacacggc gttccagctc ctcgttggat ttctgcaagg tctccgcggc gcgcacgctt
     3901 tcggtggcat cgttgaacgt gacaacaacg ccgccatccg gaatggcatt tgaccttatt
     3961 tccaggacct ggccgctgtc gcagaggcgc tcgcggaaat gctcgtgggt gatggccatc
     4021 ttgcgcaacc ggtcctcaag gctcgcctcg ccgctttcgg tggtgcggag gaattccagg
     4081 acgatctcgc gcatgggggc gccgacgcgc cccagttcat tgctgagctt gagatggtcg
     4141 tggaagctgg cgttccagct caggttcatt tcggaatcaa acaccgcaat tccctgctcg
     4201 acattgtcga tggccgattg cagcaggtcg cggttgtgct ggatggcggc ggaggcatca
     4261 tcaaggagcc gcatggcgcc gcggggatgg gcggaatggc gctcaagcag caaggccatg
     4321 acaagacgcg acgaggccac gcccacggcg cgggccagga gatgctcggc gaaacgcagc
     4381 atgcggacat cggcttcgag ggccggcttt tgcgtgacgc cgcgctgggc aaagaattcg
     4441 gcaaaggctg atctggcgcg ggcttcgccg agatagcggg cgacggtctg ctcgagcttt
     4501 tcaacagtga cggcggttcg ccagaggcgg aagcccgtgc cggtgccgac agtcagttcg
     4561 cgggtcacaa agcttgcggc ctgaatgcgt tcgatggggg tgggcaggcg gctcagggaa
     4621 aagccgacat aggcacagat attggcggca aggctccaca gcacgccatg gctcaaggca
     4681 tccaattcca tgttgaacag catccggggc cggagaaaat ccaggccgaa cgggccctgc
     4741 tcgatcaggc ccttgcccat ccagccggca tcggcgaagg acggaagcag caacgtgtag
     4801 ccccagacaa gggcgccggc cgtgagtcct gccatggcac cgcgggccgt ggcgcgtttc
     4861 cacatcaggg cgccaaaaaa ggccggggca aactgggcca cggcggcaaa ggaaatcagg
     4921 ccgacctggg ccaatgcggc ggtcgagccg atcatccggt aatagccata ggccagcacc
     4981 agcacgaggg cgatggccag ccgcctgatg gtgatcaggg tctttcccat gtcatggagg
     5041 agttccggcc gttcggtgcg ctggcggagc agcaggggca cgacgaggtt gttgcagatc
     5101 atgatggaga gcgcgatggt ttcaaccacc accatggcgg tggaggccga gaggccgccg
     5161 atgaaggcca ccatggcgaa gaacccgctc tgggcgctga ccggcagggc aagcacgaag
     5221 gtgtcgccgt cggcgctcgc gggcagcatg accagcccgg caatggcgat ggggatgacg
     5281 aagatattga tggcgacgag atacagcgga aacagccagg cggcgcggcg gatgtcggag
     5341 ggggcggca
//
LOCUS       DAORVN010000035         5325 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_10594_length_5325_cov_3.323719, whole genome shotgun sequence.
ACCESSION   DAORVN010000035 DAORVN010000000
VERSION     DAORVN010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 5325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5325
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10594_length_5325_cov_3.323719"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..616
                     /locus_tag="PKV23_01220"
     CDS             <1..616
                     /locus_tag="PKV23_01220"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HQX84080.1"
                     /translation="FGPLGSGKYTEYSHDIHKSGQFLLDVINDILDMSKIEAGRMELE
                     LTTLSLPSIIDDIMRFVGPRAAEGKVEIILELPKTCNITADRRALKQVFINLMSNAVK
                     FTPEGGQVKVTVAKTGEGARVTISDTGIGIPHNDIEKLGRPFEQVENQFTKSKGGSGL
                     GLAISKSLVDLHNGSLTIASSVGAGTTVTVELRTSPTADSSRVA"
     gene            complement(613..1128)
                     /locus_tag="PKV23_01225"
     CDS             complement(613..1128)
                     /locus_tag="PKV23_01225"
                     /inference="COORDINATES: protein motif:HMM:NF025173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HQX84081.1"
                     /translation="MSDDGTTTRFRFWPSQGSRWWTVLLIASLAFNLFIGAAMATRYF
                     THGGPQRFIGASYAQLVPRGFFAEMPSDRRRVLLDILRQYRKDFRAEREGTETVAAKL
                     ADALVNEPYDVEKVKLVVGEFADRSGKLAARGGDAALDIIGRLSPEERKVLANAIRDR
                     ASRNKGRRKSP"
     gene            complement(1125..1478)
                     /locus_tag="PKV23_01230"
     CDS             complement(1125..1478)
                     /locus_tag="PKV23_01230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX84082.1"
                     /translation="MERYDETRMNAALDKLLKHATDPAVPEGAEARLMLAIQAAGQQS
                     NVVPFQPRAKLQGWAIGLPLAAALALGIYLGSQGTLDAYFPGTITDDISAELSDPIPF
                     TGLDDAESYAEGDVT"
     gene            complement(1488..2036)
                     /locus_tag="PKV23_01235"
     CDS             complement(1488..2036)
                     /locus_tag="PKV23_01235"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HQX84083.1"
                     /translation="MLLASARRDAAAFAELVSRYYKPVYRMVWRMMNGNAETEDVAQE
                     AFVKLWQNPAQIREAKALKGWLMRVASNLAIDRMRRKLHADIEAIAEVADPRQVTGAE
                     LDEKAVTARVDQAIASLPERQKLAVTLVYFEGMSNISAASVMDISVEAIESLLTRGKR
                     ALRESLADDWRGLLDELSSKAG"
     gene            complement(2114..2566)
                     /locus_tag="PKV23_01240"
     CDS             complement(2114..2566)
                     /locus_tag="PKV23_01240"
                     /inference="COORDINATES: protein motif:HMM:NF024600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HQX84084.1"
                     /translation="MTRKALYAVLLTTGLVAAGGLAMAAEPYLPKAQKTFERIDLNRD
                     GKISVAEFTPVAEKRFLGIDVNKDNAVSTAEIDASLQAALERRRNRILANLDADRNGS
                     ISRAELDSYIEAMVAGADADSDGGVSFSEARIFKIAKWRKALQLTSAN"
     gene            complement(2642..>5325)
                     /locus_tag="PKV23_01245"
     CDS             complement(2642..>5325)
                     /locus_tag="PKV23_01245"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: protein motif:HMM:NF010706.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional [glutamine synthetase]
                     adenylyltransferase/[glutamine
                     synthetase]-adenylyl-L-tyrosine phosphorylase"
                     /protein_id="HQX84085.1"
                     /translation="ELALLCAGLHAAQSLLTQAEIMTALRHAKAKASLLIALADLGEV
                     WSVDAVTGALTRFADAMLGAAIKWLLLDATRAGKFNCVDSGDPARHSGYVALAMGKHG
                     AGELNYSSDIDLIILYDPEIAPVAEGVEPATFFVRLTKRLVTILQDVTEDGYGFRVDL
                     RLRPDPRATQVAIALEAAAIYYESMGQNWERAAMIKARAAAGDIGLGMEFLLRLKPYI
                     WRKYLDFAAIADVQSLKRQIHAVKGHGAIAVHGHNIKLGRGGIREIEFFVQTQQLIAG
                     GRNPALRGVRTLDMLDALAEAKWISPEAAAELKAAYRFMRAIEHRIQMMNDEQSHVLP
                     QDGQAFESLARLSGFASTPDFETKLRQTFELVQRHYSALFESAGELGTDTGSLVFTGG
                     EDDPETIETLERMGFKRASDISATIRGWHFGRYAATRSSKSKELLTELMPQLLLALAR
                     SGEPDLAFLAFDKFIAGLPAGVQLFSMLKANPNLLDLIATILGTAPRLAEQLSARPRV
                     LDAVLDPGFFDALPQASEIQAMIASVIPVGAALDEVVDRVRIIGREQMFRVGVRVLSE
                     TVSAVEAGASFSTIAELLLQRLHGAARQEMRMRHGEVVGGRSCVIAMGKMGGREMTAS
                     SDLDLMLIYDSAPGTENSGGTKPLSVSQYFTRLTQRLINSITAPTAEGVLFEVDMRLR
                     PSGSQGPVATSLESFRDYQKNSAWTWEKLALTRARVVSGDADLGQAISGAIIASLCAE
                     RDAEATRKDVLDMRRLMVKEQGASGLWDIKRARGGLVEVEFIAQFLQLIHARRHPEIL
                     DTNTHAALGKLTAAGLLEAGVAGELREACLLYHRITQVLRLCVTGSYDPATSPAGLNQ
                     LVARAAGVPDIRAAHSLVNDTQGRIAGLFDELIGPVG"
BASE COUNT         1042 a         1614 c         1663 g         1006 t
ORIGIN      
        1 gttcggtccg ctgggctccg gcaaatacac ggaatactcc catgacattc acaagagcgg
       61 gcaattcctc ctcgatgtga tcaatgacat cctcgacatg tcgaagatcg aggcgggccg
      121 catggagctg gaactcacca cgctgtcgct gccgtcgatc attgacgaca tcatgcgctt
      181 cgtggggccg cgcgcggcgg aaggcaaggt tgagattatc cttgagctgc ccaagacatg
      241 caacatcacc gccgatcggc gcgccctgaa gcaggttttc attaacctga tgtcgaacgc
      301 ggtaaagttc acgccggagg gcggccaggt caaggtcacg gtggcaaaga ccggggaagg
      361 cgccagggtc accatcagcg ataccggtat cggcattccg cacaatgaca tcgaaaaact
      421 gggacggccc ttcgagcagg tggaaaacca gttcaccaag agcaagggcg gctccggcct
      481 ggggctggcc atctcgaaat cgctggtgga cctgcacaat ggctcgctca ccattgcgag
      541 ctcagttggg gctggaacca cggtaacggt ggagcttcgc acctcgccta cggccgacag
      601 ttcaagagtg gcttaaggcg atttcctcct gcccttattc cggctggccc ggtcacggat
      661 ggcattggcc aggaccttgc gctcttcggg cgaaagcctg ccgatgatat caagcgccgc
      721 atcgccgccg cgggccgcca gcttgccgct ccggtcggca aattcgccga ccaccagctt
      781 gaccttttcc acgtcataag gctcgttcac aagggcatcg gccagctttg ccgccacggt
      841 ttcagtacct tcacgctcgg cgcgaaaatc cttgcggtat tggcgcagga tatcgagaag
      901 gacgcggcgc cggtcactgg gcatttcggc aaagaaaccg cggggaacga gctgggcata
      961 acttgccccg atgaagcgct gaggaccgcc gtgggtgaaa tagcgcgtgg ccatggccgc
     1021 accaatgaag agattgaagg ccagcgaggc aatcagcagg actgtccacc agcgcgagcc
     1081 ctgcgagggc caaaagcgga accttgtcgt tgtgccgtcg tcactcatgt cacgtcacct
     1141 tctgcatagc tttcggcgtc atcaaggccg gtgaagggaa ttggatcgga aagttcggcc
     1201 gaaatatcat cggtgatggt cccgggaaaa taggcgtcca gcgtaccctg ggagccgaga
     1261 taaatgccca gggccagggc cgcggccagc ggcaggccaa tggcccagcc ctgcaatttc
     1321 gcacgcggct gaaagggcac aacattcgac tgctgcccgg ccgcctggat ggccagcatg
     1381 aggcgcgcct cggcgccttc gggcaccgca ggatcggtgg catgcttcag cagcttgtcg
     1441 agcgctgcat tcatccgggt ctcgtcatat ctttccatag tacaccttca tccggctttc
     1501 gaactcaact catccaacag cccgcgccaa tcatcggcga ggctttcgcg cagggcccgc
     1561 ttgccgcggg tcaaaagaga ttcaatggcc tcaacggaga tgtccatcac cgaagccgcc
     1621 gaaatattgc tcatgccctc gaagtagacg agggtcacag ccagtttctg ccgctcgggc
     1681 aggctggcga tagcctggtc cacgcgggcc gtcaccgcct tctcgtcaag ctcggcgcca
     1741 gtgacctggc gcgggtcggc cacttcggca atcgcttcaa tgtcggcgtg gagcttgcgg
     1801 cgcatgcggt caatggcgag attcgacgca acgcgcatca gccagccttt cagcgccttg
     1861 gcttcgcgga tctgggccgg gttctgccag agcttgacga aggcttcctg ggccacgtct
     1921 tcagtttcgg cgttgccgtt catcatgcgc cagaccatgc ggtacaccgg cttgtaatag
     1981 cgcgatacca gttcggcgaa agcagcggca tcacggcgag ccgaggccag gagcaaggtg
     2041 gcatcgctcg ccgtctgcag gtccgctgaa ctggactctt gtatttcact actccatacg
     2101 cttcatcccg gcctcagttg gccgatgtca gttgcaaagc tttacgccat tttgcgattt
     2161 tgaaaatacg cgcttcggaa aaagatacgc cgccatcact gtcggcatcg gcgcctgcca
     2221 ccatggcctc gatgtagctg tcgagctcgg cgcggctgat gctgccgttc ctgtcggcat
     2281 cgagattggc cagaatgcgg ttgcggcggc gctccagggc agcctggagc gaggcgtcga
     2341 tttcagcggt gctgaccgcg ttgtccttgt ttacatcgat gccgaggaac cttttctcgg
     2401 ccacgggggt gaattcagca acgctgatct tgccatccct gttcaagtcg atgcgctcga
     2461 aggttttctg ggccttgggc agatagggct ctgccgccat ggcaaggccg ccagcggcca
     2521 caaggccggt cgtcaacagt accgcataca aagctttcct ggtcattgtt acctcctctg
     2581 aaaatcggga actcatgctt cccaaaggca agacgaaacg tgaaccagga atccggcgct
     2641 gtcagcccac ggggccgata agctcgtcaa acaggcctgc aatccggcct tgcgtgtcgt
     2701 taacgaggga atgggcggcc ctgatatcgg gcacgccggc ggcacgggcc accagttgat
     2761 tgaggcccgc cggggacgtg gcagggtcat aggacccggt cacgcaaagc ctgagaacct
     2821 gggtgatgcg gtggtaaagc aggcaggcct cccggagttc acctgccacg cccgcctcga
     2881 gcaaaccggc ggcggtgagc ttgcccaatg ccgcatgggt attggtatcg aggatttccg
     2941 ggtggcgccg ggcatggatg agttgcagga actgggcgat gaattcgact tccaccaggc
     3001 cgccgcgggc ccgcttgata tcccaaagcc cggacgcgcc ctgctccttc accatcagac
     3061 ggcgcatgtc gagtacgtcc ttgcgagttg cctccgcatc gcgttcggcg cacaggctgg
     3121 cgatgattgc cccggaaatt gcttgcccaa gatcggcatc accgctcacc acgcgggccc
     3181 gggtgagggc aagtttttcc caggtccagg cggagttctt ctgatagtcg cggaaacttt
     3241 ctaggctggt ggctacagga ccctgactgc ccgatggccg caagcgcatg tccacctcga
     3301 agagcacacc ttcggccgtt ggcgcggtga tcgagttgat gagccgctgg gtcagccggg
     3361 tgaaatactg gctcacgctc aggggcttcg tcccgccgga attttccgtt ccgggcgcgc
     3421 tgtcatagat gagcatcaga tcaagatcgg aggaagcggt catttcacgt ccgcccatct
     3481 tgcccatggc gatcacgcaa ctgcggccgc ccacaacttc gccgtggcgc atccgcattt
     3541 cctggcgggc ggcgccatgg agccgctgca gcagcagttc ggcgatggtg gaaaaggatg
     3601 cgcccgcctc gaccgcactg acggtttccg acagcacccg cacgccgacg cggaacatct
     3661 gctcgcggcc aatgatgcgg acacggtcca cgacttcatc gagtgccgcg cccactggaa
     3721 tgacggacgc tatcatcgcc tggatttcac tggcttgcgg cagggcatcg aagaagcccg
     3781 gatcaagcac cgcatccaga acgcgcgggc gggcgctcaa ctgttcggcc aggcgcggcg
     3841 cggttcccag aatggtggcg atgagatcaa gcagattggg attggccttc agcatggaaa
     3901 agagctgaac cccggcgggc aggccggcaa tgaacttgtc aaaagctagg aaggcgaggt
     3961 caggttcgcc gctgcgggcc aatgccaaga gcagctgtgg catcagttcg gtgagaagtt
     4021 ccttggactt gctgctgcgg gtggcggcgt agcggccaaa atgccagcct cggatggtgg
     4081 ccgaaatgtc gctcgcccgt ttgaagccca tgcgttccaa agtttcgatg gtttcgggat
     4141 catcctcgcc gccggtgaaa accagactgc cagtatcggt tccaagctca ccggcacttt
     4201 caaagagtgc ggaatagtga cgctgcacca gttcaaaggt ctggcgcagt tttgtttcaa
     4261 agtccggggt tgaggcaaaa ccggacaggc gcgcgaggct ttcaaaagcc tgcccatcct
     4321 gcggcagcac atggctttgc tcgtcgttca tcatctggat gcggtgttca atggcccgca
     4381 tgaagcggta ggccgccttc aattcagcgg cggcttccgg agaaatccat ttcgcttcgg
     4441 ccagggcatc gagcatgtcc aaggtgcgta cgccgcgcag cgcggggttg cggcccccag
     4501 cgatgagctg ctgggtctgc acgaaaaatt caatttcgcg gattccgccg cggcccagct
     4561 tgatgttgtg gccatggacc gcgatggcgc catggccttt caccgcatgg atctggcgtt
     4621 tcagggactg cacatcggcg atggccgcga aatccaggta tttgcgccag atatagggct
     4681 tgagccggag caggaattcc atccccaggc caatgtcgcc cgcggcggcc cgcgccttga
     4741 tcatggcggc gcgctcccag ttctgcccca tgctctcgta atagatcgca gcagcttcga
     4801 gggcgatggc aacctgggtg gcgcgggggt cggggcgcag gcgcaggtca acgcgaaaac
     4861 cgtagccgtc ttccgtcacg tcctgcagaa tggtgaccag ccgcttggtg agccgcacaa
     4921 aaaaggtggc tggctcgacg ccttccgcca cgggtgcgat ttccggatcg tagagaatga
     4981 taagatcaat gtcgctggag tagttcagtt cgccagcgcc gtgcttgccc atggcgagcg
     5041 ccacatagcc ggagtggcgc gctggatcgc cggaatcgac gcagttgaat tttccggcgc
     5101 gcgtggcatc gagaaggagc catttgatgg cggcgcccag catggcgtcg gcaaaacgcg
     5161 tgagcgcccc cgtcaccgcg tcgacggacc agacctcccc caggtcggcc agcgcaatga
     5221 gaagcgaggc ctttgccttg gcatgacgca gcgccgtcat aatctcagcc tgggtcagga
     5281 gcgactgcgc ggcgtggagg ccggcgcaga gcaacgccag ctcat
//
LOCUS       DAORVN010000036         5247 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_10853_length_5247_cov_2.886749, whole genome shotgun sequence.
ACCESSION   DAORVN010000036 DAORVN010000000
VERSION     DAORVN010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 5247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5247
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10853_length_5247_cov_2.886749"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..866)
                     /gene="rhaS"
                     /locus_tag="PKV23_01250"
     CDS             complement(<1..866)
                     /gene="rhaS"
                     /locus_tag="PKV23_01250"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0033296 - rhamnose binding [Evidence IEA];
                     GO_process: GO:0015762 - rhamnose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnose ABC transporter substrate-binding
                     protein"
                     /protein_id="HQX84086.1"
                     /translation="MTKTTRRQTLQLFTAGAAFGLAPSLFTTAARAEGKRIAMVVKNL
                     GNSYFDACAKGATEAATEVGGFEIIYTAPTKATAEEQIAVIDALIAQKVDGIIVSSND
                     ADALVPVGKKAMERGIKVISFDSAIGQEGRIMHLNASSTPLIGAKQVQMIAKTLGGKG
                     EVAILSAASTMTNQNSWIEAMKEEWKKPEYKDMPLVATVYGDDQDDKSYREMQGLVKA
                     YPNLKGVISPTTIGIRSGAKAIIDGGLVGKVFITGLGLPSEMKDAVLKGACDSFAIWN
                     PVDYGYSSTQIM"
     gene            complement(935..1735)
                     /locus_tag="PKV23_01255"
     CDS             complement(935..1735)
                     /locus_tag="PKV23_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008973041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HQX84087.1"
                     /translation="MHERERWQMILGAIKDRAVVTVRELIARTGASPATLRRDLAKLE
                     QAGQIKRIHGGVEALNFEDRPHLATRAFEASQSMRAAAKRAISRAAAALCQDNDSIII
                     NAGTTTFQMVDFLRDRKMQILTNSFPIAEALVAHSENRIIMPGGEIYREQGIVLSPFD
                     GDASQHYTASKMFMSCFSFGPLGVIEGDALIARAEAKLLGRADKLIVLADSSKFETRG
                     SMAVCPLSRVHTLITDDEAPEAVLDQVRQAGVNVIVVSAERADISFAA"
     gene            1891..3960
                     /locus_tag="PKV23_01260"
     CDS             1891..3960
                     /locus_tag="PKV23_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003507003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional rhamnulose-1-phosphate
                     aldolase/short-chain dehydrogenase"
                     /protein_id="HQX84088.1"
                     /translation="MTSSLLIPSLWDNAHAKNLDEPGLLLYRSNLLGSDLRITNFGGG
                     NTSAKVEMTDPLTKKPVQVLWVKGSGGDIASMKLDGFATLYMDKLLALNGLYKDLSQE
                     DQMVHNLNHCTFNLNPRAASIDTCLHGYVPYAHVDHVHSDAVIAIAASKDSEKLTKEV
                     FGTEMGYLPWQRPGIDLGIKLGKMAAEHPEYVGVVLGSHGLFTWAKTAKECYDTTLRI
                     INKAATWLDKNGRKPAFGGEAVKSLSPAERQAVVLRLLPEIRGRISTEEMKTGHFTDA
                     PEVLEFVNGKMLGALAPLGTSCPDHFLRTKIRPVIIPHDAEGTALDKLIANYRADYGR
                     YYDRCKHPNSPKMRDPNAVIYLVPQVGMLSFAKDKATARVAAEFYVNAINVMRGASSV
                     SSYVGLPEQEAFNIEYWLLEEAKLQRMPKAKSLAGRVAFITGGAGGIGTAIAERLMKE
                     GACVVLADIDQPSLETTVANFGKSFGKDNVRGVVMDVTREDQVTKAMDDAVKAFGGVD
                     ILVNNAGITLAANVEDTTLELWNKNMSIMATGYFLVGREGYRLMKRQGLGGSMVFIAS
                     KNAIAASPGASAYCTAKAAEVHLARCMALEGAPKGIRVNVVNPDAVLRGSKIWQGEWR
                     QQRAASNKISEDELEEHYRKRSLLQRSVFPEDIAEAVYFFASDLSAKSTGNFLNVDGG
                     NAVTFSR"
     gene            3963..>5247
                     /gene="rhaI"
                     /locus_tag="PKV23_01265"
     CDS             3963..>5247
                     /gene="rhaI"
                     /locus_tag="PKV23_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018183755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose catabolism isomerase"
                     /protein_id="HQX84089.1"
                     /translation="MTFKISPDLIGTENRKLEQAQDDDYAGLARQLDRRGIDIEAMTR
                     RAMDYAVAIPTWGVGTGGTRFARFPGLGEPRNIHDKLADCGVIQQLCRATPNVSPHIP
                     WDKVADYNALRQEAASYELGFDAVNSNTFQDQSGQKTSYKFGSLTHASKATRDQAIEH
                     NIECIEIGQKLGSTALTVWIADGANFPGQTNLNAAFDRYMDSLKAIYDKLPRNWFVFL
                     EHKLYEPAFYATVISDWGSSFMAAQELGPKAKCLVDLGHHAPNTNIEQIVARLIRAEK
                     LAGFHFNDSKYGDDDLDSGSVDPFRLFLIFNELVDAELRKAKGFAPAYMIDQSHNVTD
                     PIESLMVSAMEIVRSYVQASLVDRAALAAAQDESDVMAGHRLIKQAFNTDVSPILAEA
                     RRRKGGAIDPLSLYRTSGYRAAKAGERPVSGVASAG"
BASE COUNT         1079 a         1650 c         1474 g         1044 t
ORIGIN      
        1 atcatgatct gcgtcgagga atagccatag tcgaccgggt tccagatggc gaagctgtcg
       61 caggcgccct tgaggactgc atccttcatt tccgacggca gtcccagccc ggtgatgaac
      121 accttgccga ccagcccgcc gtcaatgatc gccttggctc ccgagcggat gccaatggtc
      181 gttggcgaaa tgactccctt gaggttggga taggctttca ccagaccctg catttcgcgg
      241 tagctcttgt cgtcctggtc atcgccatag acggtggcaa ccagcggcat gtccttgtac
      301 tccggcttct tccattcttc cttcatggcc tcgatccagg aattctggtt ggtcatggtg
      361 gaagcggccg acaggatggc aacctcaccc ttgccgccca gggtcttggc gatcatctgg
      421 acctgcttgg ccccgatcag cggcgtggag gacgcattga gatgcatgat gcggccttcc
      481 tgtccgatgg ccgagtcaaa gctgatcacc ttgatgccgc gttccatggc cttcttgccg
      541 accggcacga gagcatccgc gtcattcgac gacacgatga tgccgtcgac cttctgtgcg
      601 atgagcgcgt cgatgaccgc aatctgctct tcggcggtgg ccttggtggg tgccgtataa
      661 atgatctcga agccgccaac ttcggttgcg gcttccgtgg cgcctttggc gcaggcatcg
      721 aaatagctgt tgccgagatt cttcaccacc atggcaatgc gcttgccttc agcgcgtgcc
      781 gctgtggtga acagactggg tgccaggcca aatgcggcgc ccgcggtgaa cagttgaagg
      841 gtttgtctgc gtgttgtttt cgtcatgtct ttctcccatt tggtcggctt tggagggcgt
      901 tatcgccgtc cggtttagaa tctctttcct tcacctacgc ggcaaaggaa atatcggcgc
      961 gctccgcact gacgacgata acatttacac ctgcctgccg gacctgatcg agcacggctt
     1021 caggggcctc gtcgtcggtg atgagggtgt gaacccggct cagcgggcaa accgccatgc
     1081 tgccccgggt ttcaaacttc gaagaatcgg caaggacaat gagcttgtcg gcgcggccca
     1141 gaagcttggc ctcggcccgc gcgatcaggg catcaccctc gatcacccca agcgggccga
     1201 aggaaaagca gctcatgaac attttggatg ccgtgtaatg ctgggacgca tcgccatcaa
     1261 acggcgaaag cacaatgccc tgctcccggt agatttcacc acccggcatg atgatgcggt
     1321 tttcgctgtg cgccaccagg gcctcggcga taggaaagga gttggtgagg atttgcattt
     1381 tgcggtcacg caggaagtca accatttgaa aggtggtcgt gccagcattg atgatgattg
     1441 agtcattatc ctgacagagt gcggccgccg cccggctgat ggcccgtttt gcagcagccc
     1501 gcatggactg gctcgcctcg aaagcccgcg tcgccagatg cgggcggtct tcaaagttca
     1561 gcgcctcgac gccgccatga atcctcttga tctggcctgc ttgttcgagt ttggccaggt
     1621 cgcggcgcag ggtggcaggc gaagctccgg tgcgcgcgat gagttcgcga accgtgacga
     1681 cggcgcgatc cttgatcgcc ccgagtatca tctgccagcg ttcgcgttcg tgcatccaat
     1741 cctccaagac aatcaaaact aatcattagc aatcacgaac gtcaatactt accgcagtgc
     1801 aaaacgtcat tgcactgcaa tgaggcaatt tatgattgac gttgagcgaa cctgatcata
     1861 cggtaattca aagcaaccag gtacgcatcc atgacatcct ccctattgat cccatcactg
     1921 tgggacaatg cccatgccaa aaatctcgat gaacccggcc tcctccttta tcgctccaac
     1981 cttctaggta gcgacctgcg catcaccaat ttcggtggcg gcaatacctc cgcgaaggtg
     2041 gagatgaccg atccgctgac caagaaacct gtgcaggtgt tgtgggtgaa aggctccggc
     2101 ggcgacattg cctccatgaa actcgacggc ttcgccacgc tctacatgga caagctgctg
     2161 gccctgaatg gcctctacaa ggacctctca caggaagacc agatggtgca caacctcaat
     2221 cactgcacct tcaatctcaa tccccgcgcc gcatccattg atacctgcct gcatggttat
     2281 gttccttacg cccatgtcga ccatgtccat tccgatgcgg tgatcgccat tgccgcctcg
     2341 aaggattccg aaaagctcac caaggaggta tttggcaccg agatgggtta cctgccgtgg
     2401 cagcgccccg gcatcgatct cgggataaaa ctcggcaaga tggctgccga acacccggaa
     2461 tatgtgggcg tggttctggg cagccatggc ctgttcacct gggccaaaac cgcaaaggaa
     2521 tgctacgaca cgacccttcg catcatcaac aaggccgcca cctggctcga caagaatggc
     2581 aggaagcctg cctttggcgg cgaagccgtc aaatctctca gcccggcgga acgccaggcc
     2641 gtggtcttgc gcctgctgcc cgaaattcgc gggcggattt ccaccgaaga gatgaagacc
     2701 gggcatttca ccgatgcacc cgaggtgctg gaattcgtga atggcaaaat gctgggcgcc
     2761 ctggcgcccc tgggcacctc atgcccggac catttcctcc gcaccaagat ccgtcccgtg
     2821 attattcccc atgatgccga agggacagcc ctcgacaagc tgatcgccaa ttaccgcgcc
     2881 gactatggcc gctactatga tcgctgcaag caccccaact cgcccaagat gcgcgacccc
     2941 aatgcggtga tctacctcgt gccgcaggtc ggcatgctgt ccttcgccaa ggacaaggca
     3001 accgcccggg tcgccgccga attctacgtc aatgccatca atgtcatgcg cggcgcgtca
     3061 tcggtgtcct cctatgtggg acttcccgaa caggaggctt tcaacattga gtactggctg
     3121 cttgaggaag ccaagctgca gcgcatgcca aaggccaaga gcctggccgg gcgcgttgcc
     3181 tttatcaccg gcggggccgg cggcatcggc acggctatcg ccgagcgcct gatgaaggaa
     3241 ggggcctgcg tggtcctcgc cgatatcgac cagccttcac ttgaaacaac cgttgccaat
     3301 ttcggcaagt ctttcggcaa ggacaatgtg cgcggcgtgg tcatggacgt gacccgcgag
     3361 gaccaggtga ccaaggccat ggatgatgcg gtcaaggcct tcggcggcgt cgacatcctc
     3421 gtcaacaatg ccggcatcac ccttgccgcc aatgtcgagg acaccaccct tgagctgtgg
     3481 aacaagaaca tgtcgatcat ggccaccggc tatttccttg tgggccgcga aggctaccgc
     3541 ctgatgaaac gccagggtct cggcggttcc atggtgttca ttgcctccaa gaacgccata
     3601 gcggcctcgc caggggcttc ggcctattgc accgccaagg ctgccgaggt tcatttggcc
     3661 cgctgcatgg ccctcgaagg ggcgccaaag ggcatccgcg taaatgtcgt caacccggac
     3721 gccgtcctgc gcggctccaa gatctggcag ggcgaatggc gccagcagcg cgccgcctcc
     3781 aacaagattt ccgaggatga attggaggag cattaccgca agcgctccct cctgcagcgt
     3841 tcggtgtttc ccgaggacat tgccgaagca gtctatttct tcgcctccga cctctcggcc
     3901 aaatccaccg gaaatttcct caacgtcgat ggcggcaatg ccgtcacctt ctcgaggtag
     3961 ccatgacctt taaaatctca cccgacctga tcggcacgga aaacaggaag ctggagcagg
     4021 cccaggacga cgattacgct gggctggcgc gccagttgga tcgccggggc atcgacatcg
     4081 aggccatgac caggcgcgcc atggactacg cggtggccat cccgacctgg ggggtgggaa
     4141 ccggcggcac ccgctttgcc cgctttccgg gcctgggcga accccgcaac atccacgaca
     4201 agctggccga ctgcggcgtg atccagcaac tctgccgcgc cacacccaac gtgtcccccc
     4261 atatcccgtg ggacaaggtg gccgactaca acgcgctgcg ccaggaagcg gcatcctatg
     4321 aactgggctt cgacgcagtg aactccaaca cctttcagga tcagagcggg cagaagacat
     4381 cctataaatt cggctccctc acccacgcca gcaaggcgac acgtgaccag gccatcgagc
     4441 acaacataga gtgcatcgaa atcggccaga aactgggctc caccgccttg accgtctgga
     4501 tcgccgacgg ggcgaatttc ccgggccaga ccaacctcaa cgctgccttt gaccgctaca
     4561 tggactcgct caaggcgatc tatgacaaac tgcccaggaa ctggttcgtg ttcctcgaac
     4621 acaagctcta tgagccggcc ttctatgcaa ccgtcatttc cgattggggc tcgagtttca
     4681 tggcggccca ggaacttggc ccaaaggcca aatgcctggt tgacctcggc caccacgcgc
     4741 ccaacaccaa catcgagcag atcgtggcgc gcctcatccg ggccgagaag ctggcgggct
     4801 tccatttcaa tgattcgaaa tatggcgacg atgacctgga ttcaggctcc gtcgatccct
     4861 tccgcctgtt tctgattttc aacgaactcg tcgatgccga gttgcggaaa gccaagggct
     4921 tcgccccggc ctatatgatc gaccagtcgc acaatgtgac cgatcccatt gaaagcctga
     4981 tggtctcggc catggaaatt gtccggtcct acgtgcaggc gtcactcgtt gaccgcgcgg
     5041 ccctcgccgc cgcccaggac gagagcgacg taatggccgg tcaccgactg atcaagcagg
     5101 ctttcaacac cgacgtcagc cccattctcg ccgaagcccg ccgccgcaag ggcggcgcca
     5161 tagacccctt gagcctgtac cgcacctccg gctatcgtgc tgcaaaagcc ggggaaagac
     5221 ccgtcagtgg agtagcctcc gccggca
//
LOCUS       DAORVN010000037         5237 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_10879_length_5237_cov_3.407179, whole genome shotgun sequence.
ACCESSION   DAORVN010000037 DAORVN010000000
VERSION     DAORVN010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 5237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5237
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10879_length_5237_cov_3.407179"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1327)
                     /locus_tag="PKV23_01270"
     CDS             complement(<1..1327)
                     /locus_tag="PKV23_01270"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HQX84090.1"
                     /translation="MSSARIGYLAILLTGLLLCLGVRYEANRQYLAAWQQYLEARQAD
                     SRDYTKRVSSFFSTLNDNLGILSFLPSVRKIDSFDHSVGLDGRGTEAHGNNLGADGRE
                     TIQQVYNILAKNASASRLYIVPLDFDSKHLDMVTGRLERPILELDRIRSRSKTIGVDP
                     DSITPARPDSFRIPEFAEVQTQLNWFKVNYPTISRLKTFAVPMISGKEIATGGESGLI
                     FSAPFYDPDGKLAGSVAAVIPTAVLRSITGTENYSLISPRAEYISVPLARDKNIRLML
                     RAAHSVPTATTIFSETVTIDTNDARGKWQLNINHPVAGFYTGPRFAARKDFENGSYLA
                     LGLLTLLGLGWHRTNMKRSQEFKDSSAALQLVNDDITRLNLDLAENMRKLREAHDEII
                     KKGKLAQMGQLVATVAHELRNPLSAVRTSAYLLKRKLTGHSVNVDTQLER"
     gene            1468..3000
                     /locus_tag="PKV23_01275"
     CDS             1468..3000
                     /locus_tag="PKV23_01275"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006936374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HQX84091.1"
                     /translation="MQEILAKLELRRAEARAGGGEKRSKAQHARGKLTARERIDLLLD
                     EDSFEEFDMFVEHRSTDFGMEKSKIPGDGVVTGWGTVNGRVVYVFAKDFTVFGGSLSE
                     SHARKITKIQDMALQNRAPIIGLFDAGGARIQEGVNALGGYGEVFQRNVLASGVIPQI
                     SVIMGPCAGGDVYSPAMTDFIFMVRDTSYMFVTGPDVVKTVTNETVTAEELGGALVHT
                     TRSSIADKAFDNDVEALLQMRRLIDFLPASNTDPIPEIPSFDDAGRMEASLDTLIPAN
                     PNQPYDMKELILKIADEGDFFEIQEAHAKNIIVGFARLAGRTIGIVANQPLVLAGVLD
                     SDAGRKAARFVRFCDCFNIPIVTFVDVPGFLPGTAQEYGGLIKHGAKLLFAFAEATVP
                     KITVITRKAYGGAYVVMSSKHLRADLNYAWPSAEIAVMGSRGAAEIIYRSELGDKDKI
                     AARVKDYEDRFANPFVAAERGFLDEIIMPHSTRKRIARALDMLRHKELNNPWKKHDNI
                     PL"
     gene            3010..4221
                     /locus_tag="PKV23_01280"
     CDS             3010..4221
                     /locus_tag="PKV23_01280"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020187464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HQX84092.1"
                     /translation="MHLLRTLLTRFVKVGTLEVYDVEGKPHVFSGKPGPKVAIRLKDR
                     KLYRTLFLNPELYAGEAYMDGTLVMEGTTIRQFLEFAEINGNNLRKHPLQRILHKGLK
                     GIRKLHQRNDAKSARAHVHHHYDLSNELYKSFLDEDMHYSCAYFASDTDSLEVAQQNK
                     LRHIAAKLDLKPGQRVLDIGCGWGGMALYLAKVADVEVLGVTLSTEQLALARQRAIER
                     GLDNRVRFELKDYRDVEGTFDRIVSVGMFEHVGIAHYDEFFAKVRDLLKPDGVALLHS
                     IGRKGGPGATGPWLRKYIFPGGYSPALSEAMASVERSKLWVTDIEILRLHYARTLRAW
                     EQRFQANRKKIMTFMDERFCRMWEFYLIASELSFTHGSHMVFQIQLAKQRETLPLTRD
                     YMAKAEAQLKA"
     gene            complement(4218..>5237)
                     /gene="rimO"
                     /locus_tag="PKV23_01285"
     CDS             complement(4218..>5237)
                     /gene="rimO"
                     /locus_tag="PKV23_01285"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HQX84093.1"
                     /translation="QYEAVVGAVHNALPPVHDPKYDLMPPQGLRLTPKHYAYLKISEG
                     CNNRCSFCIIPSIRGDLVSRPVASVLYEAERLVKAGVKELLVISQDTSAYGVDTKYAT
                     SKFHGREVRAKFYDMAKELGDLGAWVRMHYVYPYPHVDDVIPLMSEGKILPYLDIPFQ
                     HAAPNVLKAMRRPANQEKVLDRLASWRKICPDIAIRSTFIVGFPGETDADFNYLLDWM
                     KEARLERVGCFKYEPVSGARANDLGLPQVPDKVKAERYHRFMEAQQEISAEIFQSKVG
                     REIDVLVDAIDLENEEAVARSPWDAPEIDGNVFLPGETSLKPGDMVRVRIVEAEEYDL
                     IGEQI"
BASE COUNT         1128 a         1485 c         1459 g         1165 t
ORIGIN      
        1 tgcgctccag ctgcgtatca acgttcaccg agtggccagt gagctttcgc ttgagcagat
       61 aggctgaggt tctgaccgcc gacagcggat tgcgaagctc atgggcaacg gttgcgacca
      121 gctgccccat ctgcgccagt ttgccttttt tgatgatttc atcgtgcgcc tcgcgaagct
      181 tgcgcatgtt ttcggccagg tcgaggttta gccgtgtgat gtcatcgttc acaagctgca
      241 gcgccgcgct gctatccttg aactcctgcg atcgcttcat atttgtgcgg tgccagccca
      301 ggcccaggag ggttagaagt cccagcgcaa ggtaggagcc gttctcgaaa tcctttctcg
      361 ctgcaaagcg cggtcccgtg taaaatcccg ccaccggatg attgatgttc agttgccatt
      421 tgccgcgtgc gtcattggtg tcgatcgtga cagtttctga gaaaatggtt gtggcagttg
      481 gcacggaatg cgccgcgcgc agcattaatc gaatgttttt atcgcgcgcc agcggaactg
      541 aaatgtattc ggcgcgcggg ctgatcagcg aatagttttc agttcccgtg atgctgcgca
      601 gaacggccgt aggtatcacc gccgcaacgc tgcctgcaag tttgccatcc ggatcataga
      661 acggagccga aaaaatcaat cctgactcgc cgcccgtggc gatctctttc ccgctgatca
      721 tcggaacagc aaaggtcttc aacctgctga tggttggata gttcaccttg aaccagttga
      781 gttgcgtctg cacttccgcg aattctggaa ttcggaacga gtcggggcgg gctggtgtga
      841 tggaatcagg gtcaacgcca atggtctttg acctgctgcg tatgcggtcg agttcaagaa
      901 taggccgctc caggcggccc gtcaccatat caagatgctt ggaatcaaaa tcgaggggca
      961 cgatatagag ccgggaagca gaggcgtttt tggctaggat gttgtagact tgctggattg
     1021 tttcccggcc atcggcgcca aggttgttgc catgtgcctc tgttccccgg ccgtcaaggc
     1081 cgacactgtg gtcaaagctg tcaattttgc gcacgctggg caggaagctc agaattccca
     1141 gattatcgtt cagggtggaa aagaatgagc tgacccgctt ggtatagtcc cgcgaatccg
     1201 cctgccgggc ctccaggtat tgctgccagg ccgcaagata ttggcgattt gcctcatagc
     1261 gtacgccaag gcacagcagc agtcccgtca gaaggatagc cagataccca attcttgctg
     1321 acgacacctt tggcaaattt ctcccctgag gaattatagc tgccgcagac tggttccttt
     1381 gtggttaaat ctgggttaaa tgtgtaacaa gttcatgaat ttggccgcta agagaagcca
     1441 tggttaaggc caagccgggg gaggcaaatg caggaaatcc tagctaaact ggagcttcgc
     1501 cgcgctgaag cccgggcggg cggtggcgaa aagcgttcaa aagcccagca cgcccgcggc
     1561 aaacttacgg cccgcgaacg catcgacctc cttcttgatg aggactcctt tgaagagttc
     1621 gacatgttcg tcgagcaccg cagcaccgat tttggcatgg aaaagtcaaa aatccccggc
     1681 gatggcgtgg tcaccggctg gggcacggtg aatggccggg ttgtgtatgt gtttgccaag
     1741 gactttacgg tgtttggcgg ctcactctcc gaatcccatg cccgcaagat caccaagatc
     1801 caggacatgg ctctgcagaa tcgcgcgccc atcattggcc tgtttgatgc tggcggtgcg
     1861 cgcatccagg agggggtgaa cgccctcgga ggctatggcg aagtctttca gcgcaatgtc
     1921 ctggcttccg gcgtcatccc gcagatctcg gtgatcatgg gaccctgtgc cggcggcgac
     1981 gtctattccc cggcgatgac cgacttcatc ttcatggtgc gtgataccag ctacatgttt
     2041 gtgaccggtc ccgacgtggt gaagacagtc accaatgaaa cggtgacggc cgaggagctt
     2101 ggcggcgccc tggtgcacac cacaaggtcc tccattgccg acaaggcgtt cgacaatgat
     2161 gtcgaggcgc tgttgcaaat gcgccgcctg atcgattttt tgcccgcgtc gaacaccgat
     2221 ccaattcctg agattccaag ctttgatgat gccgggcgga tggaggcttc cctcgataca
     2281 ttgattcccg ccaatcccaa tcagccctat gacatgaagg aactgatcct caagattgcc
     2341 gacgagggcg atttcttcga aatccaggaa gcccacgcca aaaacattat tgtcggtttt
     2401 gccaggctcg ccggccgcac catcggcatc gtggccaatc agccgctggt gctggcgggc
     2461 gttcttgaca gcgacgccgg gcgcaaagct gcccgcttcg tgcgcttctg cgactgtttc
     2521 aacattccca ttgtgacctt cgttgatgtg ccgggatttc ttcccggcac cgcccaggaa
     2581 tatggcggcc tcatcaagca cggcgccaaa ctgctgtttg ccttcgccga ggccaccgtg
     2641 ccgaaaatca ccgtgatcac ccgcaaggct tatggcggcg cttatgtggt tatgtcgtcg
     2701 aagcatttgc gggccgacct gaactacgcc tggccctccg ccgaaattgc cgtgatggga
     2761 tcgcgtgggg cagccgaaat catttaccgc agcgaactcg gcgacaagga caagattgcc
     2821 gcccgcgtga aggactatga ggatcgcttt gccaatccat tcgtggcggc ggagaggggc
     2881 tttctcgatg aaattatcat gccccactca accagaaaac gcatagctcg ggcccttgat
     2941 atgctgcgcc ataaggaatt gaataaccca tggaaaaaac acgataatat tcctctttag
     3001 gagatgggca tgcatctgct gagaaccttg ctgacacgtt ttgttaaggt cggaacgttg
     3061 gaagtgtacg atgtggaagg caaaccccat gtcttttccg gaaagccggg ccccaaggtt
     3121 gcaatccgcc tcaaggaccg gaaactttac cggactctgt ttctcaatcc cgaactctac
     3181 gccggcgaag cctacatgga tggcacgctt gtcatggaag gcaccacaat ccgccaattc
     3241 ctcgagtttg ccgaaatcaa tggaaacaac ctgcgcaagc atcccttgca gagaattctg
     3301 cacaagggtc tcaaaggcat tcgcaagctg caccagcgca atgacgcaaa atccgcccgc
     3361 gcccatgtgc atcaccatta tgatctgtcc aacgaactct acaaatcatt cctcgacgag
     3421 gacatgcatt attcctgtgc ttattttgca agcgacacgg acagcctcga agtggcccag
     3481 caaaacaagc tgcgccacat cgcggcaaag cttgatctaa aaccgggtca acgtgtcctg
     3541 gacattggct gcggctgggg cggcatggcg ctctatctgg caaaggtcgc ggatgtggag
     3601 gttttgggcg tgaccctgtc aaccgagcaa ctggccctgg cgcgccagcg ggcaatcgag
     3661 cgcggcctcg acaatcgcgt gcgctttgag ttgaaggact atcgcgatgt tgagggcact
     3721 tttgatcgca ttgtctcggt gggcatgttt gaacatgtgg gcattgccca ctacgacgag
     3781 ttttttgcca aggtgcgcga tctgctgaag cctgatggcg ttgcccttct gcattccatc
     3841 ggccgcaaag gcgggcctgg cgccactggc ccctggctgc gcaagtatat tttccccggc
     3901 ggttattccc cggcgctttc ggaagccatg gcaagcgtcg aacgctccaa gctgtgggtg
     3961 acggacatcg aaatcctgcg gctccactac gccaggacgc tcagggcctg ggagcagcgc
     4021 tttcaagcca atcgcaagaa gatcatgacc tttatggatg aacgcttctg ccgcatgtgg
     4081 gaattctacc ttatcgcaag cgaactgagc tttacccacg gttcccacat ggtgttccag
     4141 attcagcttg ccaagcagcg cgaaaccttg cctttgacga gggactacat ggcaaaggcc
     4201 gaagcccagt taaaggctta gatctgctcg ccaatcagat catactcttc ggcttcgacg
     4261 atccgcacac gcaccatgtc gccgggcttc agtgatgtct cgccaggaag gaacacgttg
     4321 ccatcaattt ccggcgcatc ccacggcgaa cgcgccactg cctcttcatt ttccagatca
     4381 atggcatcca ccagaacgtc gatttcccgg ccgactttcg actggaaaat ttcagcggaa
     4441 atctcctgct gtgcttccat aaaacgatgg tagcgctcgg ccttcacttt atccggcact
     4501 tgcggcagtc ccaggtcatt ggctctggcc cctgacaccg gctcgtattt gaaacagcca
     4561 acacgctcca gcctcgcttc cttcatccag tccagaagat agttgaaatc cgcatcggtc
     4621 tcgccgggaa accccacgat aaaggtggag cggatggcaa tatccggaca aattttacgc
     4681 catgaggcca aacggtccag aaccttttcc tggttcgccg gccgccgcat cgccttcaac
     4741 acgttggggg ccgcgtgctg gaacggaata tcgagatagg gcagaatttt tccttccgac
     4801 atgagcggga tcacatcatc gacatgggga taggggtata catagtgcat ccgcacccat
     4861 gcgcccagat cgcccagttc ctttgccatg tcatagaact tggcgcgcac ctcgcggcca
     4921 tggaatttgc tcgtggcgta cttggtatcc acgccatagg cagacgtgtc ctggctgatc
     4981 accagcaatt ctttcacgcc cgctttcaca agtcgctcgg cctcgtaaag taccgaagcc
     5041 acgggccgcg aaacaaggtc accgcgaatg gatgggatga tgcagaagga gcagcggttg
     5101 ttgcagcctt cggagatttt cagataggca taatgcttgg gcgtcaggcg caggccctgg
     5161 ggcggcatga ggtcatactt gggatcatgc accggtggca gcgcattgtg cacggccccc
     5221 accacggcct catattg
//
LOCUS       DAORVN010000038         5173 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_11141_length_5173_cov_2.767683, whole genome shotgun sequence.
ACCESSION   DAORVN010000038 DAORVN010000000
VERSION     DAORVN010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 5173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5173
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11141_length_5173_cov_2.767683"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1957
                     /locus_tag="PKV23_01290"
     CDS             <1..1957
                     /locus_tag="PKV23_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003521446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HQX84094.1"
                     /translation="ESIGAEDALEEVPRRQRRAPRRQYKIQEVIKRRQIILVQVVKEE
                     RGNKGAALTTYLSLAGRYCVLMPNTARGGGISRKITDAGDRRRLKEVARDVEVPQGMG
                     VIVRTAGAQRTRPEIKRDFDYLMRLWENVRDLTLHSTAPALVYEEGSLIKRSIRDLYT
                     KDVDEVFVEGEAAYREAKDFMKMLMPSHAKNVKLYKDTRPLFSKHQTETQLDALYSPT
                     VTLPSGGYIVMNQTEALVSVDINSGKATREHNIEDTALKTNLEACDEIARQLRLRDLA
                     GLVVIDFIDMEENRNNRAVERRLKDALKNDRARIQVGRISHFGLMEMSRQRLRPGLLE
                     GSSRPCPHCEGRGIVRSISSCGLSVLRAIEEHLISRKAEHLTVKCTREVAFYVLNEKR
                     DNLLTIEQNYGISVFIVPSDEVKGSQAVIERASDRVVAARKIQFVPVKLDAYVEDAEE
                     EETEAEVEAEEEGVDVQAESAAEPENGEEGSDSQRGRRRRRGRRGGRRGGRDREDEPR
                     DKIAGEGDGNIDAPRQAAEADDSAGDDVVSPNEAEMSSAPDDSSEKEGSERGPARRRD
                     RWGRNRNGRNRNGRPAAEGESNGHAHAESPAEIRAPQPERQPTPVIAIQEIIAQPVEP
                     RKWQPPAATVTPTEVAKPKAGWWSKRG"
     gene            2052..3437
                     /locus_tag="PKV23_01295"
     CDS             2052..3437
                     /locus_tag="PKV23_01295"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HQX84095.1"
                     /translation="MIRNLTAVFLALALAISGLADSGMAATKKKPRGPSLPLIRDAEI
                     EGLLRLYSRASFKAAGLNPAAVRVYIINNDKINAFVAGGQRMFIHTGLLTRTKTPNEI
                     IGVLAHETGHIAGGHLARLGIELDRASVQSIIGTLVGVAAVMGGAAAGQKGATQAGQG
                     IMIGSQGMAQRGLLSYQRGMESSADQAALKYLKATEQSPQGMLSLFQLLANESLASLE
                     NADPYLLSHPMPLERIRNLELEAKKSAFFNKDDAPQLLLRHHLMQAKLAGFMQSPQYV
                     FQRYPSADKSLPARYARSISMFRKGDTKNAIPIIDSLIRDIPENPYFWELKGQALLEG
                     GAPAKALASLRQANKLLPNSGLIQILYAQALIATEDRANIKQALNLLTLAKKTERDSV
                     DVYKYSALAYGYLNDLPRAELATAEMAMMQGDTALASEKAKRVIGQFKHGSPEWMKAN
                     DVLNFTSKERN"
     gene            3487..4245
                     /locus_tag="PKV23_01300"
     CDS             3487..4245
                     /locus_tag="PKV23_01300"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HQX84096.1"
                     /translation="MHAAVRTLLLATTLLWGLGASAHAAEFNDAQRKEMESIIKDYLL
                     ANPDILREMGQLLEQQERLAEDQQRKGALVANADKIFRDGTDFVAGNPDGKVTMVEFF
                     DYNCGWCKKGFSEVMALIESDKDLRFVLKEFPIFGGDSDYAAQAAIAAGKQGKYWDLH
                     VAMFSHEGKITKETVDELAAAQGLDMAQLKKDMESPETAAMLERNRTLAQSLAINGTP
                     AFIIDDKLVPGYLPKAELVSAINDVRAKGGCSLC"
     gene            4318..4767
                     /gene="aroQ"
                     /locus_tag="PKV23_01305"
     CDS             4318..4767
                     /gene="aroQ"
                     /locus_tag="PKV23_01305"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013420961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HQX84097.1"
                     /translation="MKPIYILNGPNLNLLGLREPTVYGQETIADLEARCAAKAKALGQ
                     DVVFRQTNTEGELIGWVHEAQGKASGIIINGAGYTHTSVALHDALKAVNIPTIEVHLS
                     NVYAREPFRHHSYVSPVAHGVICGFGGQSYELALDGIAQILKSSGKR"
     gene            4771..>5173
                     /locus_tag="PKV23_01310"
     CDS             4771..>5173
                     /locus_tag="PKV23_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018063631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, biotin carboxyl carrier
                     protein"
                     /protein_id="HQX84098.1"
                     /translation="MPPKKVPPLRGQTKSASPELDLIRTLADILNETGLSEIELEQKG
                     SRVRVSRTVTAAATVQQHAPVHHAPAPSQALSAPVAKVPDTDHAGTVKSPMVGTIYMA
                     SAPGNPNFIEIGSQVKQGQTLLIIEAMKTIAF"
BASE COUNT         1211 a         1534 c         1522 g          906 t
ORIGIN      
        1 cgaatccatc ggcgcggaag acgcgctgga agaagtgcca agacgccagc gccgggcgcc
       61 gcgccgccag tacaagatcc aggaagtcat caagcgccgc cagatcatcc tcgtgcaggt
      121 ggtcaaggag gaacgcggca acaagggtgc ggcgctcaca acctatctct cgctcgccgg
      181 acgctattgc gttctcatgc cgaacacagc gcgcggcggc ggcatttccc gcaagatcac
      241 cgacgcgggc gaccgccggc gcctcaagga agttgcccgt gacgtggaag tcccgcaggg
      301 catgggggtg attgtccgca ccgccggggc gcaacgcacc cggccggaaa tcaagcgtga
      361 ctttgattat ctcatgcgcc tttgggaaaa tgtgcgcgat cttacgcttc attcaacggc
      421 ccctgccctt gtctatgagg aaggcagcct gatcaagcgt tcgatccgcg atctctatac
      481 caaggacgtg gacgaagtgt ttgttgaagg cgaagcggcc taccgcgaag ccaaagattt
      541 catgaagatg ctcatgccat cgcatgccaa gaacgtgaaa ctttacaagg acacgcgccc
      601 gctgttttcc aagcatcaga cggaaaccca gcttgacgcc ctctactcgc caacggttac
      661 gctgccgtcg ggcggctata tcgtgatgaa ccagacggaa gccctggttt cggtcgacat
      721 caactcaggc aaggcaacgc gcgaacacaa tattgaagac acggcgctga agaccaatct
      781 ggaagcctgc gatgaaattg ccaggcagct gcggctgcgc gatcttgccg gccttgtcgt
      841 catagacttc atcgacatgg aagagaaccg caataaccgc gcggtggagc ggcgcctgaa
      901 ggatgccctg aaaaatgacc gggcccgcat ccaggtgggc cggatcagcc atttcggcct
      961 gatggaaatg tcgcggcaaa ggcttcggcc ggggctgctt gaaggttcgt cacggccctg
     1021 cccgcattgc gaaggacgcg gcattgtccg ttcgatctcc tcgtgcgggc tttccgtgct
     1081 gcgcgccatc gaggaacacc tgatcagccg caaggcagag catctcacgg tgaagtgcac
     1141 gcgcgaagtg gccttctatg tgcttaacga gaagcgcgac aatcttctga ccattgaaca
     1201 gaattacggc atttccgtct tcatcgtgcc aagcgacgag gtcaagggat cgcaggcggt
     1261 catcgagagg gccagtgacc gggtcgttgc agcacgcaag atccagttcg tgcccgtgaa
     1321 gctcgatgcc tacgtggaag acgccgagga agaggaaacc gaagccgaag tcgaggctga
     1381 ggaagagggt gtagacgttc aagccgagtc tgctgctgaa cctgaaaatg gcgaagaagg
     1441 ctccgactcg cagcgcggca gacggcggcg gcgcggccgc cggggcggca ggcgcggcgg
     1501 acgcgaccgt gaagatgagc cgcgggacaa aattgccggc gagggagatg gcaacatcga
     1561 tgcgccgcgc caagccgctg aagcagacga ttccgcaggc gatgatgtcg tttcgcccaa
     1621 cgaagcggaa atgagttcgg cgcccgatga ttccagcgag aaggagggct ccgagcgcgg
     1681 acctgcacgc cggcgcgacc gctggggacg caacagaaac gggcgcaacc ggaatggccg
     1741 ccctgccgct gaaggcgaaa gcaatggcca cgcccatgcg gaaagcccgg cagagatacg
     1801 cgctccgcaa ccggaacgtc agccgacccc ggtcatcgca atccaggaaa ttatcgccca
     1861 accggttgag ccgcgaaagt ggcaaccacc ggcagccaca gtgacgccaa ccgaagtggc
     1921 caaacccaag gctggctggt ggagcaaacg cggctgaaga tcacgagcct caaactcgcc
     1981 gtatttgaca gagaaacagc cctcttgaat gcggcgggta atgggcccgc atgcgacctg
     2041 ttgagtgaac catgattcgc aatttgacag cggttttcct tgccctcgcg ctcgccattt
     2101 cgggcctcgc ggattctggc atggcagcta ccaagaagaa gccgcgtgga ccgtcgcttc
     2161 cgctcattcg cgacgcggaa atcgaaggcc tgctgcggct ctactcgcgg gccagcttca
     2221 aggcggcagg gctgaacccc gcggcggtgc gcgtctacat catcaacaat gacaagatca
     2281 atgccttcgt cgcaggcggc cagcgcatgt tcattcatac gggcctgctg acgcggacca
     2341 aaactccgaa cgaaatcatc ggtgttctgg cgcatgagac gggccatatc gcaggcgggc
     2401 atttggcgcg cctgggcatt gaactcgacc gcgccagtgt ccagtcaatt atcggaacgc
     2461 tggttggcgt ggcggccgtc atgggcggcg ccgccgcggg gcaaaaaggc gcgacccagg
     2521 ccggacaggg aatcatgatt ggatcccaag gcatggccca gcggggtttg ctctcttacc
     2581 agcgcggcat ggaatcatct gccgaccagg cagcactcaa atacctcaag gcgacggaac
     2641 agtctccgca gggcatgctc tcgttgttcc agttacttgc caatgaatcg ctggcttcgc
     2701 tggaaaacgc cgacccttac ctgctgtcac atcccatgcc gctggaaaga attcgcaacc
     2761 ttgagctgga agcaaaaaaa tccgccttct tcaataagga cgacgcgccg caactgctgc
     2821 tccgccatca cctgatgcaa gccaagctgg ccggcttcat gcagtcgccg caatatgtct
     2881 ttcagcgcta tccctcagcg gacaagagcc tgccggcacg ctatgcccgg tcgatttcaa
     2941 tgtttcgcaa gggcgacacc aagaatgcca tcccgatcat cgacagcctc ataagggata
     3001 ttccggagaa cccctatttc tgggagctca agggccaggc cctgctggaa ggcggcgctc
     3061 cggcgaaggc gcttgcgtcg ctcaggcagg ccaacaaact gttgccgaac agcggcctca
     3121 tccagatcct ctatgcgcaa gccctgatcg ccacggaaga tcgcgcaaat atcaagcaag
     3181 cactgaacct cctgacgctg gccaagaaaa ccgagcggga ttcagttgat gtctacaagt
     3241 attccgcgct tgcctatggc tatctgaatg acttgcccag ggccgaattg gcaactgccg
     3301 aaatggccat gatgcagggc gacacggcgc tggcgtccga aaaagccaag cgcgtcatcg
     3361 ggcaattcaa acacggctcg ccggaatgga tgaaggccaa cgacgttctg aatttcacca
     3421 gcaaagaacg gaattgaccc ggaacctgcc tttcaggcaa atagactgga aacacaagga
     3481 ttgaacatgc acgctgccgt caggactcta ttgcttgcga ccacgctgct gtgggggctc
     3541 ggcgccagcg cccatgccgc cgaattcaat gatgcccagc gcaaggaaat ggaatccatc
     3601 atcaaggatt accttctggc aaatcctgac attctcaggg aaatgggcca acttctggag
     3661 cagcaggaac ggcttgcgga agaccagcaa agaaaaggcg ccctggttgc caatgccgac
     3721 aaaatattcc gtgacgggac ggatttcgtg gccggcaatc ccgatggcaa ggtcacgatg
     3781 gtcgagttct tcgactataa ctgcggttgg tgcaaaaagg gcttttccga agtcatggcg
     3841 ttgatagagt ccgacaagga tctgagattc gtcctgaagg aattcccgat attcggcgga
     3901 gattcggact atgcggctca ggcggccata gccgccggca agcagggcaa atactgggac
     3961 ctgcatgtgg ccatgttcag ccacgaagga aaaatcacca aagagacagt cgacgaactc
     4021 gcagcggcgc agggccttga catggcgcag ctcaaaaaag acatggagag tccggagacg
     4081 gccgccatgc tggagcgcaa ccgcaccctc gcccagtcgc ttgccatcaa tggcacgccg
     4141 gctttcatta tcgatgacaa gctggttccc ggctacctgc ccaaagccga actggtcagc
     4201 gccattaacg atgtccgggc gaaaggcggc tgttcactct gctgatgccg ggcgcttccc
     4261 tgcccgccat tgaccgccta taacgcgtta cacatcacag attcttatgg cagggcaatg
     4321 aaaccgatct acatcctaaa cggccccaac ctgaacctgc ttggcctgcg cgaacccacc
     4381 gtctatggcc aggaaaccat cgctgacctg gaggcgcgct gtgccgccaa ggccaaggcg
     4441 cttggacaag acgttgtgtt caggcagacc aatacggaag gcgaactgat cggctgggtg
     4501 catgaggcgc aaggcaaggc ttccgggatc atcatcaatg gagccggcta tacccatacc
     4561 tcggttgcct tgcatgacgc cctgaaggcg gtgaacatcc cgaccatcga agttcacctg
     4621 tccaatgttt acgcccgcga accgtttcgc catcacagct atgtttctcc tgttgcccac
     4681 ggggttattt gcggctttgg tggacaaagt tatgaactgg ccctcgacgg cattgcgcaa
     4741 atcctgaaat caagcggaaa gaggtaaaga atgcccccga aaaaagtgcc gccattgcgt
     4801 ggccagacaa agtccgccag tccggaactt gatcttatcc ggaccctcgc ggacatcttg
     4861 aatgaaacag ggctttctga aatcgaattg gagcaaaagg gttcgcgcgt ccgcgtgtcg
     4921 cggacggtaa cggcggcggc aaccgtgcag cagcacgcac cggtgcatca tgcgcccgcg
     4981 ccgtcacagg cgctgtcggc gcccgttgcc aaggttcccg acacggatca tgcgggaacg
     5041 gtaaaatccc ccatggtggg aacgatctac atggcctcgg ccccgggcaa cccgaatttc
     5101 atagaaatcg gctcacaggt caaacagggc cagaccctgc tgatcattga agccatgaaa
     5161 accattgcct ttg
//
LOCUS       DAORVN010000039         5163 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_11172_length_5163_cov_3.093579, whole genome shotgun sequence.
ACCESSION   DAORVN010000039 DAORVN010000000
VERSION     DAORVN010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 5163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5163
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11172_length_5163_cov_3.093579"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1891)
                     /gene="dnaK"
                     /locus_tag="PKV23_01315"
     CDS             complement(<1..1891)
                     /gene="dnaK"
                     /locus_tag="PKV23_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019199296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HQX84099.1"
                     /translation="MAKVIGIDLGTTNSCVAVMEGKTPKVIENAEGARTTPSIVAFNA
                     EGETLVGQPAKRQGVTNPENTFFAIKRLIGRRMEDPMVAKDKKLVPYNIVKADNGDAW
                     VESRGQKYAPSQISAFTLTKMKETAERYLGEPVTQAVITVPAYFNDSQRQATKDAGKI
                     AGLEVLRIINEPTAAALAYGLDKAEAGLIAVYDLGGGTFDVSVLEIGDGVFEVKSTNG
                     DTFLGGEDFDMRIVDYLADEFKKEQSIDLRKDKLALQRLREAAEKAKIELSSATQTEI
                     NLPFITADASGPKHLTLKLTRSKLEALVDDLIQKTVEPCRQALKDAGVTAGQIKEVVL
                     VGGMTRMPKVIEVVKQFFGKEPHKGVNPDEVVAIGAAIQAGVLQGEVKDVLLLDVTPL
                     SLGIETLGGVFTRLIDRNTTIPTKKSQVFSTADDNQTAVTIRVFQGEREMAADNKMLG
                     QFDLMGLPPAPRGVPQVEVTFDIDANGIVNVSAKDKATNKEQQIRIQASGGLSDKEIE
                     KMVKEAEANATEDKKRKEVVEAKNHAESLMHSTQKSVVELGDKITPADKEAVEAAVAD
                     LKSALEGEDAEVIKEKTNVLAQAAMKLGEALYKQSGGGASTDGEVPNMEKDDAEVDIV
                     DADFTE"
     gene            2071..2661
                     /locus_tag="PKV23_01320"
     CDS             2071..2661
                     /locus_tag="PKV23_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB"
                     /protein_id="HQX84100.1"
                     /translation="MRYLPEFLDRSAQEQVLRDLREAVAQAPLFNPVMPRTGKPFSVR
                     MTNLGPLGWFSDRAGYRYQASHPATGLPWPAIPDSILAVWNRVSNYPHPPEACLVNFY
                     GSDAKMGLHQDRDEEDLEAPVVSISLGDTALFRVGGTVRGGKTQSLKLASGDVLILEG
                     PTRLAFHGIDRVINGTSTLLKDGGRINLTLRRVRKP"
     gene            complement(2802..4082)
                     /locus_tag="PKV23_01325"
     CDS             complement(2802..4082)
                     /locus_tag="PKV23_01325"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HQX84101.1"
                     /translation="MTIPKKAGPRRQRVLDTINHRTPDQVPVDFGGSAVTGMHASCVA
                     ALRDHYGLERRLVKICEPYQMLGLIEDDLKEAIGVDITPVFSRTNMFGFENRDWKPFL
                     FNDLEVLVPGGFRTMTEPETGGTLIYPRGDTSAPPSGHMPEGGFFFDAIIRQEPLDLK
                     NLRAEDNLEQYSLLTDKQLDEIEDDARATNTGEYAVFASLTNTSLGDISEVPAIDLTH
                     PKGVRDIAEWYVLTASRPDIVHQIFERQVEIGIANLERIHARIGDAIDVIYLCGTDFG
                     TQTSSFCSVKTFDSLWKPHYAELCNWVHKNTSWKTFKHSCGSSERFFESLIDAGVDII
                     NPVQCSAKGMDPRMLKDKYKGRLVFWGGGVDTQQTLPFGTPADVRAQVLERCEIFSEG
                     GGFIFDSVHNVQAGTPVANIVAMIDAVKEFNAAA"
     gene            complement(4109..4759)
                     /locus_tag="PKV23_01330"
     CDS             complement(4109..4759)
                     /locus_tag="PKV23_01330"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="corrinoid protein"
                     /protein_id="HQX84102.1"
                     /translation="MAFVEKLYDAIVDGSEAEALEAVREELRAGTDPMLLITDSMIPA
                     MDDVGQLYQEEQYFVPELILAGRAMKAAMEPLRPLLAFSGAKPAGVIVAGAVKGDLHD
                     IGKNLVITMLEGAGFKVIDLGTDVKPEKFVAAIREHNPQIVCMTALLTVTMMAMKTTI
                     MAIEAAGLRGLVKILVGGAPLNVRFANEIGADGYGETSTDAVALARQMVVSSQHAN"
BASE COUNT          951 a         1537 c         1510 g         1165 t
ORIGIN      
        1 cttccgtgaa gtcggcatcg acgatgtcaa cttcggcatc gtccttttcc atgttcggca
       61 cttcgccgtc ggtggacgca ccgccgcctg attgcttgta caaggcttcg cccaacttca
      121 tggccgcctg ggccaggaca ttggtctttt ccttgatcac ctcggcatcc tcgccttcaa
      181 gggccgactt gaggtcggca accgccgctt caacagcttc cttgtcggcc ggcgtgatct
      241 tgtcgccgag ttcaaccacg gatttttgcg tcgagtgcat gaggctttcg gcgtggttct
      301 tggcttccac cacctccttg cgcttcttgt cctcggtggc attggcttcc gcttccttca
      361 ccatcttctc gatctccttg tcggagaggc cgcctgaggc ctggatgcgg atttgctgtt
      421 ccttgttggt ggccttgtcc ttggccgaga cattgacgat gccgttggcg tcgatgtcga
      481 aggtcacttc cacctggggc acgccgcgcg gtgcgggggg gaggcccatc aagtcgaact
      541 ggcccagcat cttgttgtcg gccgccattt cacgttcgcc ctggaagacg cggatggtca
      601 ctgccgtctg attgtcatca gcggttgaga acacctggct tttcttggtg gggatcgtgg
      661 tgttgcggtc gatcaaacgg gtgaacacgc cgcccagggt ttcaatgccc agggacaggg
      721 gcgtcacgtc gagaaggaga acatccttca cttcgccctg cagcacgccc gcctggatgg
      781 cagcaccgat ggccaccact tcgtccgggt tcacgccctt gtggggctcc ttgccgaaga
      841 actgctttac cacttcgatg accttgggca tgcgcgtcat gccgccgacg aggaccactt
      901 ccttgatctg gcctgcggtg acgcctgcat ccttcagggc ctggcggcag ggctcaacgg
      961 tcttctggat gaggtcatcc acgagggcct cgagcttgga gcgcgtcagc ttcagggtga
     1021 gatgcttcgg accggaagcg tccgccgtga taaagggcag gttgatttcg gtctgggttg
     1081 cggatgacag ttcgatcttg gccttttcag ccgcctcgcg caggcgctgg agggccagct
     1141 tgtccttgcg cagatcgatg gactgctcct tcttgaattc atcggcgagg taatcgacga
     1201 tgcgcatgtc gaagtcttca ccaccgagga aggtgtcgcc gttggtggac ttcacttcga
     1261 atacgccgtc gccgatttca agaaccgaga cgtcgaaggt gccgccgccc aaatcataaa
     1321 cggcgatgag gccggcctcg gccttgtcga ggccataggc aagcgcggcg gcggtgggtt
     1381 cgttgatgat gcgcagcact tcaaggccgg cgatcttgcc tgcatccttg gtggcctggc
     1441 gctggctgtc gttgaaatag gccggaacgg tgatgacagc ctgggtgacg ggttcgccaa
     1501 gatagcgttc cgccgtttcc ttcatcttgg tgagggtaaa ggccgagatc tgcgagggcg
     1561 catatttctg cccgcggctt tcaacccagg catcgccatt gtcggccttg acgatgttgt
     1621 agggcacaag cttcttgtcc ttggccacca tggggtcttc catgcggcgg ccaatgaggc
     1681 gcttgattgc aaagaaggtg ttttcggggt tggtgacgcc ctgccttttg gcgggctggc
     1741 cgaccagggt ttcgccttcg gcgttgaaag cgacaataga tggggttgta cgggcccctt
     1801 ccgcattttc gattaccttg ggagtcttgc cctccatgac ggcgacgcaa gagtttgtcg
     1861 ttccaaggtc gataccaata actttagcca tgttttttct cctctaggca ggcagcatgc
     1921 gaaagcccca tcaggcgctt aaagtcccgc tgcctcctct gtcacccgca tatggttcgt
     1981 cttttccctg attccaaggg aaaaatcgtt cgggcggtat atagtgggat gtttccttgg
     2041 cagcaaggga gcaggcgggt aaaaataccc atgcgatacc ttccggaatt ccttgaccgc
     2101 agcgcccagg aacaggttct gcgggacctg cgggaagcgg tcgcacaggc cccgcttttc
     2161 aacccggtga tgccccggac gggcaaaccg ttctcggtac gcatgactaa tctggggccc
     2221 ctgggctggt tttccgacag ggccggctac cgctaccagg ccagccatcc ggccacggga
     2281 ctgccctggc ccgccatccc tgacagcatt ctggccgtat ggaacagggt ttcaaactat
     2341 ccccatccac cggaggcctg ccttgtcaat ttctatggga gtgacgccaa aatggggctg
     2401 caccaggacc gggacgaaga ggatctcgaa gccccggtgg tttccatttc gctgggcgac
     2461 accgccctat tccgcgtggg cggcacggtg cgcggcggaa agacgcaatc gctgaaactg
     2521 gcctcgggcg atgtgctgat cctggaaggg cccacacggc ttgcctttca cggcatcgac
     2581 cgggtgatca acggaacttc gacgctctta aaagacggcg gccgcatcaa tctcaccctg
     2641 agaagagtga ggaaaccctg agacattctc cctcacctga ggcgctccgg cgccagccaa
     2701 agcctcgaag ggccggtgga ggcgggtatt gatgcttcga caattcgttg aaacccggcg
     2761 gttcacatct tgatggcccg tcgtgacttg cgcaaccgtt ttcaggccgc cgcattgaat
     2821 tccttcaccg cgtctatcat tgccacgatg ttcgcgaccg gcgttcccgc ctgaacattg
     2881 tgaacagagt cgaagatgaa acccccgcct tctgagaata tctcacagcg ctccaggacc
     2941 tgcgcccgca cgtccgctgg cgtgccaaac ggcaacgttt gttgtgtatc gacgcccccg
     3001 ccccagaaca caaggcgacc cttgtacttg tccttgagca ttcgcggatc catgcccttg
     3061 gccgagcatt gcacggggtt gatgatgtca acgcctgcat caatcagcga ctcgaagaac
     3121 cgctccgatg atccgcagga gtgcttgaac gttttccaag acgtattctt atggacccag
     3181 ttgcacagtt ccgcgtagtg cggtttccac aggctatcga aggtcttgac tgagcagaaa
     3241 ctcgacgtct gcgtgccgaa gtcggtgccg cagagataga ttacgtcgat ggcgtcgccg
     3301 atgcgggcat gtatcctttc gagattggcg ataccgatct cgacttggcg ctcaaaaatc
     3361 tgatgcacaa tgtccggtcg gctagcggtc agcacgtacc actccgcaat atcgcgcacg
     3421 ccctttggat gtgtgaggtc gattgccggc acttcggaaa tatcgccgag gctggtgttt
     3481 gtcagtgagg cgaacaccgc gtattcgcct gtgttggtgg cgcgcgcatc atcctctatc
     3541 tcatcgagtt gcttgtcggt gagcagcgag tattgctcca aattgtcttc ggcgcgcagg
     3601 tttttgagat cgagcggctc ctgacggata atcgcatcga agaagaagcc tccctcgggc
     3661 atgtggccgc tgggtggtgc gcttgtgtcg ccgcgtggat agatcaacgt gccgccagtc
     3721 tcgggctcag tcatcgtgcg gaaaccgccg ggcacgagca cctcaagatc attgaacaaa
     3781 aagggcttcc agtccctgtt ctcgaaacca aacatgttgg tgcggctgaa caccggcgtg
     3841 atgtctacgc caatcgcttc cttgagatca tcctcgatga ggcccagcat ctgatagggc
     3901 tcacagatct tcacgagccg cctttccagt ccgtaatggt cacgcaaagc ggcaacgcag
     3961 ctcgcgtgca tgcccgtgac ggcgctgcca ccaaaatcga ccggcacctg gtcaggcgtg
     4021 cggtggttga tcgtgtcgag gacacgctgg cgccgcggac cggctttctt tgggatggtc
     4081 attgttcatc ctcctcgggc agcagcactc agttggcgtg ctgggacgac acgaccattt
     4141 ggcgcgcgag cgcaaccgca tcggtcgagg tctcgccata tccgtcagcc ccgatttcgt
     4201 tggcgaacct cacgttgagg ggggcgccgc ccacgaggat cttgacgagg ccacgcaggc
     4261 ctgccgcctc tatcgccatg atagtcgttt tcatcgccat catcgtgacg gtcagcaggg
     4321 ctgtcatgca gacgatctgc gggttgtgct cgcgtatcgc cgccacaaat ttttccggtt
     4381 tcacgtcggt gcccaggtca atcaccttga agcccgcgcc ttcgagcatg gtgatcacga
     4441 gattcttgcc aatgtcatgc agatcaccct ttacggcgcc cgccacgatg acgccggcgg
     4501 gtttcgcccc ggagaatgcg agcaggggcc tgagcggttc catcgccgcc ttcatggcgc
     4561 ggccggccag tatcaactca ggaacaaaat actgttcttc ctggtagagc tgcccgacat
     4621 cgtccattgc cgggatcatc gagtcagtta tcaggagcat ggggtcggtg cccgcacgga
     4681 gttcttccct tacggcctca agcgcctctg cttcactgcc gtcaacgatt gcatcgtaga
     4741 gcttttcgac aaacgccatg gctccctctc caattgcaac tgcggatctt gttcccgctg
     4801 ccgccttccc cgcaacgtcc cgacaattgg taacgattat gcctgcagga cttcggcgtc
     4861 aatgcggaat gcgggaaaat atcagaaacc agcagcaaca gaaccggctt ccactgcaat
     4921 aaatgaaagc ggacgtgatc aggctacgga tggatttgga ggcgaacggc ggaaggccgc
     4981 tcggctctgc tcaaagacgc ggccactata aaatctctcc atggtcgccc tccgaggcga
     5041 ccatccagtg actgccgcaa gctcgaagtc tgggtggtcg ggtcgagccc gaccatggag
     5101 aattatgtta gcccttccag ccgttcagct ttgcattgtt ctcggcgttc ttccttgaac
     5161 tga
//
LOCUS       DAORVN010000040         5158 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_11196_length_5158_cov_3.868313, whole genome shotgun sequence.
ACCESSION   DAORVN010000040 DAORVN010000000
VERSION     DAORVN010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 5158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5158
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11196_length_5158_cov_3.868313"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..520)
                     /locus_tag="PKV23_01335"
     CDS             complement(<1..520)
                     /locus_tag="PKV23_01335"
                     /inference="COORDINATES: protein motif:HMM:NF013328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HQX84103.1"
                     /translation="MPELPEVETVMRGLGPVLVGRSIAAVELRRAGLRFPFPAGFAAR
                     LKGRRVVSLQRRAKYILVNLDDGHCLLVHLGMTGRFTVLDVNAPPNNLGEFYYEQGAV
                     DAGSGPHDHVVILLDNGVRVVYTDHRRFGVMDLLHENEMAGHKLLKGIGVEPLGNEFS
                     ADFLAARFRKKSA"
     gene            595..1359
                     /gene="ubiE"
                     /locus_tag="PKV23_01340"
     CDS             595..1359
                     /gene="ubiE"
                     /locus_tag="PKV23_01340"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013499930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HQX84104.1"
                     /translation="MVEPQENDVDFGFRRVGENEKQGLVNEVFSKAAERYDQMNDLMS
                     GGLHRFWKDDLVTLLNPPRNPTAFKVLDVAGGTGDVAFRIARAGGAGTHVTVADISPE
                     MVHEGKKRAEREGLLERCDFTVGNAESLAFPDQSFDAYTIAFGIRNVTHIDRALAEAH
                     RVLKPGGHFLCLEFSHVDMELLAKAYDAFSFTVIPAVGKVVTGDGQPYRYLVESIRKF
                     PRQDEFKAMIDAAGFARTSYRNLSGGIVAIHTGWRI"
     gene            1359..2900
                     /gene="ubiB"
                     /locus_tag="PKV23_01345"
     CDS             1359..2900
                     /gene="ubiB"
                     /locus_tag="PKV23_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-polyprenylphenol 6-hydroxylase"
                     /protein_id="HQX84105.1"
                     /translation="MISTVKNIWRLARAGRTMARYDFFLQPEQVSELPWFARSLLKTA
                     KFGAAAPIVDSGSRVSAALSSLGPSYIKLGQFLSTRPDVIGAKRAFELKSLQDRLPPF
                     SMAEARDTIRANIGQSAEELFTEFGEPVAAASIAQVHKALTRDGEQVAVKILRPGVEK
                     RFADDLSSFFFAARMMERFSREARRLRPVAAVEMLDHSMKLELDLRMEASAISEMAKA
                     TADDPNFRLPAVNWPLTGRQVLTTEWIEGTPISDIATLRAKGFDLKALGDNIIQSFLR
                     HAIRDGFFHADMHQGNLFVDAKGHLVVVDFGIMGRLSEGDRLFLAEILYGFITRDYER
                     VSQVHFDAGYVPRDQDVADFAQALRAIGEPIMGLAAHEISMARLLTQLFEVTGQFNMV
                     TQPQLLLLQKTMVVVEGVARTLNPELNMWVTAEPVVRSWIERKLGPIGKLEGAVQSAA
                     SLGRLASMLPDVVTEIVQSGSIKLDADTTNRIAIAHHHANRFQRYAIMAGAAALVAIA
                     LKLVL"
     gene            2931..4148
                     /gene="coaBC"
                     /locus_tag="PKV23_01350"
     CDS             2931..4148
                     /gene="coaBC"
                     /locus_tag="PKV23_01350"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HQX84106.1"
                     /translation="MTAVTGKTILLIIGGGIAAYKSLELIRLLTSKGVTVRSIMTKAA
                     EEFVTPLSVASLTRQKVHTALFNLTDEIEMGHIELSRAADLLVVAPATADLMAKMAHG
                     QANDLASTTLLATDKPVLIAPAMNVRMWHRAATQRNLAQLKRDGILLVGPNEGDMACG
                     EYGLGRMAEPAEIVAAIEAALAPRAQPLKGKKVLVTAGPTREAIDPVRYISNYSSGKQ
                     GYAIAAAAVALGAETVLISGPVDLAIPPGVQMIPVTSAVEMLATSERELPCDIAIFTA
                     AVADWRVAEEKGEKIKKDKNGLPDLKFVENPDILQTIARRASKRPGIVVGFAAETENL
                     TANAQDKLKKKGCDLIVANDVSKEHGVFGGEQNTVQLVSTSGVESWGILSKQDVAQRL
                     MAQLAAQLATGKA"
     gene            4145..4585
                     /gene="dut"
                     /locus_tag="PKV23_01355"
     CDS             4145..4585
                     /gene="dut"
                     /locus_tag="PKV23_01355"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008193513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HQX84107.1"
                     /translation="MTALRIMRLANGANLPLPAYESPGAAGMDLRAAEDAVLKPGARF
                     LMPTGFAIALPDSHEAQVRPRSGLAVKHGVTVLNAPGTIDSDYRGEIKVPLINHGTAE
                     FIIRRGDRIAQMIVAAVVRAALQEVAALDDTARADNGFGSSGVR"
     gene            4585..>5158
                     /locus_tag="PKV23_01360"
     CDS             4585..>5158
                     /locus_tag="PKV23_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012112505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HQX84108.1"
                     /translation="MALSPDEVERYARHIVMPEVGGPGQAKLVNAKVLVIGAGGLGAP
                     ALLYLAAAGIGTIGICDNDTVSLSNLQRQVVHTTAAVGEPKTKSAKATLAAINPHPKI
                     IEHRQRLDASNALDIIRQYDIVADGCDNFPTRFLVSDACYFANKPLVSAAVGQFDGQI
                     STFKPYEKAPSGEPLPTYRCFIGELPERGLF"
BASE COUNT         1063 a         1537 c         1540 g         1018 t
ORIGIN      
        1 gggcggattt tttccggaat cttgctgcca gaaaatcggc gctgaattca tttccgagag
       61 gctccacgcc gatgcctttc agaagcttgt ggcctgccat ttcattttcg tgaagaaggt
      121 ccatcacgcc gaagcggcgg tgatcggtat aaacaacccg cacgccatta tcgagcagga
      181 tgaccacatg gtcatgggga ccggaaccag cgtcaacggc gccctgctca taataaaatt
      241 cgcccaggtt gtttggcggt gcgtttacat ccagaaccgt aaagcgcccg gtcatgccga
      301 gatgcaccaa aaggcagtgg ccatcatcaa ggttaaccag aatatacttg gcccggcgct
      361 ggagactcac aacacgtctg cccttcagac gggcggcgaa gcccgcggga aagggaaagc
      421 gcaggccggc ccttcgcagc tccaccgcgg cgatggaccg cccaacaagg acggggccaa
      481 gcccgcgcat aacagtttca acttcgggga gttccggcat gattattaag caataagcgt
      541 taagccgcca cactggcaat agaactcatg aaacctttgg tttatgatgg ctccatggtt
      601 gaacctcaag aaaatgacgt ggacttcggc tttcgccggg tgggcgaaaa cgagaagcaa
      661 ggcctggtca acgaggtctt ttccaaggcc gccgaacgct atgaccagat gaatgacctg
      721 atgtccgggg gcttgcaccg gttctggaaa gacgatctgg tcaccctgct caacccgcca
      781 cgaaacccca ccgccttcaa ggtgctggat gtggcgggcg gcacgggtga cgtcgccttc
      841 cgcattgccc gggcgggtgg cgctggaacc catgtgacgg ttgccgatat ctcacccgaa
      901 atggtgcatg agggcaaaaa acgggcagag cgcgaaggcc tgcttgagcg ctgcgatttc
      961 actgtgggca atgccgaaag cctggccttt cccgaccaga gctttgatgc ctatacgatt
     1021 gcctttggca ttcgcaatgt cacccatatc gaccgggcac tggcggaagc gcaccgcgtg
     1081 ctgaaaccgg gcgggcattt tctttgcctg gagttttcgc atgtcgacat ggaactgctt
     1141 gcgaaggcct atgacgcctt ttcattcaca gtcattcccg ccgtcggcaa ggttgtcacc
     1201 ggcgatggcc agccctaccg ctacctggtc gaaagcatcc gcaaatttcc caggcaggac
     1261 gagttcaagg ccatgatcga tgcggcaggc tttgcccgca cgagctaccg caatctttcc
     1321 ggcgggatcg tcgccattca cacgggctgg cggatctgat gatttcaacg gtgaaaaaca
     1381 tctggcggct ggcccgcgcc ggacggacca tggcgcgcta tgattttttc ctgcagccgg
     1441 aacaggtttc cgaactgcca tggtttgcac ggtcgcttct gaagacggcc aaatttggcg
     1501 ccgccgcgcc aattgttgat tccggcagcc gggtaagcgc ggcgctctcg tcgcttgggc
     1561 ccagctacat caagcttggg caattcctgt ccacccgccc tgacgtgatc ggggccaagc
     1621 gggcctttga actgaaatcc ctgcaggacc ggctgccgcc cttcagcatg gcggaagcgc
     1681 gcgacaccat ccgtgccaat attggccaga gcgcggaaga gctgttcacg gagttcggcg
     1741 agccggtcgc cgccgcatcg attgcccagg ttcacaaggc cctgaccagg gatggcgaac
     1801 aggtcgccgt caagattctg cgccccggag tagagaagcg cttcgccgat gacctctcaa
     1861 gtttcttttt tgcggcgcgg atgatggagc ggttctcgcg cgaggcgcgg cgcttgcgcc
     1921 ccgtggcggc cgttgaaatg cttgatcatt ccatgaagct tgaactcgac ctgcgcatgg
     1981 aggcttcggc catttcggaa atggccaagg ccaccgccga tgatccaaac ttccgcctgc
     2041 cggcggtcaa ttggccgctc accggccgcc aggtgctgac gacagagtgg atcgagggca
     2101 cacccatctc ggatatcgca acgcttcggg cgaaaggttt tgatctcaag gcgctgggtg
     2161 acaacatcat ccagagcttc ctgcgccacg ccatccgtga tggcttcttt catgccgaca
     2221 tgcatcaggg gaatcttttc gtcgatgcca agggccatct cgtcgtggtt gatttcggca
     2281 tcatgggaag actgagcgaa ggcgaccgcc tgttcctcgc tgaaatcctt tatggcttca
     2341 tcacgcggga ctatgaacgg gtctcccagg ttcacttcga tgcgggctat gtgccgcgcg
     2401 accaggatgt ggccgatttc gcccaggcgc tccgcgccat cggcgagccc atcatgggcc
     2461 ttgcggcaca tgaaatctcc atggcgcggc tgctcaccca gctttttgag gtgaccgggc
     2521 agttcaacat ggtgacccag ccgcaacttc tgctgttgca gaagaccatg gttgtggtcg
     2581 aaggagtcgc ccgcaccctt aaccccgaac tcaacatgtg ggtcaccgcc gaacctgtgg
     2641 tgcgaagctg gattgagcgg aagctggggc ccatcggcaa gctggagggg gccgtgcaaa
     2701 gtgccgcttc gcttggccgc ctggcctcca tgctgcctga tgtggtgacc gaaatcgtgc
     2761 aatccggcag catcaagctc gacgccgaca cgacaaaccg tattgccatc gcccaccacc
     2821 atgccaaccg tttccagcgc tatgcaatca tggcaggggc ggctgcgctg gtggcaatcg
     2881 ccctcaaact cgtcctttaa gaaagagcgt ttaatcgcta cattggcccc atgaccgctg
     2941 tcaccggaaa gaccatactt ctcatcattg ggggcggaat tgccgcctat aaatcccttg
     3001 aattgatccg gctattgacg agcaagggcg tgacggtgcg cagcatcatg accaaggcgg
     3061 cggaagagtt tgtcacgccc ctgtcggttg ccagccttac ccggcagaag gttcacaccg
     3121 cgcttttcaa tctcaccgat gaaatcgaaa tgggccatat cgagctgtcg cgggcggcgg
     3181 acctcctggt cgtggcgccg gccacggcgg acctcatggc caagatggcc catggccagg
     3241 ccaatgacct tgcctccaca acacttcttg ccacggacaa gcctgtcctg atcgcacccg
     3301 ccatgaatgt gcggatgtgg catcgcgcgg cgacccagcg caatctcgcg caattgaagc
     3361 gcgatggcat tctcctggtc gggcccaatg aaggggacat ggcctgcggt gaatatggat
     3421 tgggccgcat ggcggagcct gccgaaatcg ttgcagccat tgaagcggct ctggcccccc
     3481 gggcacaacc gctcaagggc aagaaggttc tggtcacggc gggcccgacg cgcgaagcca
     3541 ttgatccggt gcgctacatt tccaattatt cctcaggcaa gcagggctat gccattgccg
     3601 ctgcggcggt cgcactcgga gcggaaacgg ttctgatttc gggaccggtc gatcttgcga
     3661 ttcctcccgg tgtgcaaatg attccagtga caagtgccgt tgaaatgctc gcaaccagcg
     3721 agcgggagct tccctgcgac atcgccatct tcacggcggc agtcgccgac tggcgcgtgg
     3781 cggaggagaa aggcgagaag atcaagaaag acaagaacgg ccttcccgat ctcaaatttg
     3841 tggaaaatcc cgacatcctg cagacgattg ccaggcgcgc ctcaaagcgg ccaggaatag
     3901 ttgttggatt cgctgccgaa acggaaaatc tcaccgccaa tgcgcaggac aagctgaaga
     3961 aaaaaggctg tgacctgatc gtcgccaatg atgtgagcaa ggagcacggg gtttttggcg
     4021 gcgagcaaaa caccgtgcag ctcgtgtcga cgtctggcgt tgaaagctgg ggaatcctgt
     4081 cgaagcagga tgtggcgcag cgcttgatgg cgcaacttgc ggcgcaactg gctacgggca
     4141 aggcatgaca gcgttgcgga tcatgcgtct ggccaatggc gcaaaccttc cgctgcccgc
     4201 ctatgaatca ccgggggccg ccggcatgga tcttcgagcc gcagaggatg cggtattgaa
     4261 acctggcgcg cgtttcctga tgccgacggg ttttgccatc gcacttcctg acagccacga
     4321 ggcgcaggtg cggccacgct cggggcttgc ggtcaagcac ggcgtcaccg tcctgaacgc
     4381 gccaggcacc atcgacagcg attaccgcgg cgaaatcaag gtgccgctga tcaatcacgg
     4441 cacggcagaa ttcatcatca ggcgcggcga caggattgcc cagatgattg tcgccgccgt
     4501 ggtgcgcgcg gctttgcagg aggtcgcggc gcttgatgat acggcccggg ccgacaatgg
     4561 ctttggctcg tccggagtcc gctgatggct ctttctcctg atgaagttga acgctacgcc
     4621 cggcacattg tgatgcccga ggttggcggt cccgggcagg caaagcttgt caacgcaaaa
     4681 gtcctcgtga tcggggccgg aggtctgggc gcgccggcgc tgctctatct cgcggcggcg
     4741 ggaatcggca ctatcggcat ctgcgacaat gacacggtga gcctttcaaa ccttcagcgc
     4801 caggttgtcc atacaactgc ggcggtgggt gagcctaaaa cgaaaagcgc caaggcaacg
     4861 ttggcggcaa tcaatccgca tcccaaaatc atcgagcacc gccagcgcct tgatgcttcc
     4921 aatgcgctcg acatcatccg tcaatatgac atcgttgccg atggctgcga taatttcccc
     4981 acgcggtttc tggtcagcga cgcctgctac ttcgctaaca agcctctggt atcggccgcc
     5041 gtgggccagt tcgacgggca gatttcaacc ttcaagccct atgaaaaagc gccatctggc
     5101 gaaccgttgc ccacctatcg ctgcttcatc ggcgagttgc cggaacgggg gcttttcc
//
LOCUS       DAORVN010000041         5151 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_11220_length_5151_cov_2.990777, whole genome shotgun sequence.
ACCESSION   DAORVN010000041 DAORVN010000000
VERSION     DAORVN010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 5151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5151
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11220_length_5151_cov_2.990777"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(142..654)
                     /locus_tag="PKV23_01365"
     CDS             complement(142..654)
                     /locus_tag="PKV23_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018689105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HQX84109.1"
                     /translation="METLLRLSGGIDRLSEFIGRQVRWLILAAVLVSAVNAIIRKSFD
                     MSSNAWLELQGALFGAVFMFAAAYVLQKNGHVRIDALSSHLSKRTRDWIDLFGHVVFL
                     LPFCLLMVWLGVPYFWESLRDGENSSNAGGLVVWPVKFFILGGFALLLAHAISEIIKS
                     WAVIKGLRDD"
     gene            complement(736..1584)
                     /locus_tag="PKV23_01370"
     CDS             complement(736..1584)
                     /locus_tag="PKV23_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008120523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HQX84110.1"
                     /translation="MRATAVITTPCIITVAITGSVPRKEHNPAVPITIDEQVDSTRAA
                     FEAGASITHVHVRKDDQTPTSDPERFAALLAGLRKACPGLIVQFSTGGRSGKGSERGG
                     MLHLGCDMASLATGSCNFPTIIYENSPDLIRDLAAKMLAHKVKPEIEVFDLSMLYQAL
                     RLAEEGLLQKPLHVQFVFGVKNAMPAIRPAFDFMVDEIARLAPEATWTAAGIGRHQLE
                     VNHWCLERGGHCRTGLEDNIRWDGAGLAPSNAALVARVAELCRSYGRHPASVAEARSL
                     LSLPAL"
     gene            complement(1566..3032)
                     /locus_tag="PKV23_01375"
     CDS             complement(1566..3032)
                     /locus_tag="PKV23_01375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX84111.1"
                     /translation="MTHAKLCLQSTFYRSFLIACFAVLIAVLLPTAPRAQQASVPQTC
                     EVQTKSCCNTCGSCDSGPRCPAGYTIYDDYCLPECPGGYLRYPGYPGLCLPPCDHGCP
                     EGYDHVPLPSCPEGYHRNLSNPDECLPDRRIQIPDNCPEGLSYSEETGRCSPNCPDGT
                     YLAENGLCRSYYDKACPEGYQRNARTGACIPGGDWPDDYRWICLPSCPEGFTRDIYHP
                     TRCLPPPETCPFGFENFRQQCVPVCEQGTTRNSYGYCLPPRCEDGSYPNLRGVCSPPS
                     CPEGYETIRGKCHAPCPQGLVRNPNNPEQCERIPQDEPECPEGTRLNPQTDECERVPP
                     PPPPTVKCKQGLEYNPKTKKCEPPPRVETTCPKGFIKLKNGKCQRIVEQPPEEQGCPR
                     GFALNRNTGECEPIRLNIPKACPEGTVFDRKRQRCVRVVIEREPEFEPQDDDQPTLRL
                     PKIDLNNNLLNQQNQQNTQKRCPKGMAPDKKGRCVPLQ"
     gene            3186..4565
                     /locus_tag="PKV23_01380"
     CDS             3186..4565
                     /locus_tag="PKV23_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006333107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family N-terminal
                     domain-containing protein"
                     /protein_id="HQX84112.1"
                     /translation="MRHAQLTSLDLPDFGSPMTEPELHRDIYAARLKQLFARMAASSL
                     DALVIYGDREHAANISWATGYDPRFEEAICIIVPGRAPTLLAGNEGFPYAEMAIGSFD
                     RVLWQPLSLMGQPRGKYRDLASVLRESGMKKDMRIGLAGWKGFETDDGVFDPHWFETP
                     HYLVEALNGFGTVTNAALLFMHPTDGLRVINEVEQLARFEYAATLASNAVRRLILGAK
                     PGMSEYAACRNMAFDGFPQSVHVNMSSGPRAKYGPPSPSMRVIEKGDPIVIGVGLMGA
                     LNCRAGFMVAEASELPANIRDYVDKLVAPYFAAAAAWYETIGIGVEGGVLYEAVMSRL
                     ADPFFGIGLNPGHLIHLDEWVHSPIKQNSRMRLASGMALQCDIIPATGTPYFMSNIED
                     GVALADAPTRAAFQAKFPEAWKRIESRRAFMTEKLGITLKPEVLPFSNIPAWLPPFWL
                     SPGKVMAMR"
     gene            complement(4566..>5151)
                     /locus_tag="PKV23_01385"
     CDS             complement(4566..>5151)
                     /locus_tag="PKV23_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018097044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HQX84113.1"
                     /translation="RIKAMTGVSKAKSEVKASILRLFSYGAWADKYDGAVHSPPLRGV
                     ALAMNEPLGVVGVICPDEAPLLGFISLVAPLIAMGNRAVVVPSERHPLAATDFYQVLE
                     TSDVPAGVINIVTGGRDTLLKTLAEHDDVDGLWVFGSKENSASAEKLSAGNLKRTLVD
                     HGLATDWFDSASSEGPVLLRHAVQVKNIWIPYGE"
BASE COUNT         1022 a         1611 c         1518 g         1000 t
ORIGIN      
        1 tgcgagtagg gcgcgagctc aactcccacc acaaagaata tgaggccgtt tgccgccagt
       61 gaaaaggcca ccgggtagcc cagcagcagg aacgccacca gcgatgcaaa catgatcggc
      121 gcaagattat gggcgataag ctcaatcatc gcgcagaccc ttgatcaccg cccagctctt
      181 gatgatttca gagatcgcgt gcgccagaag cagcgcgaat ccgccgagga tgaagaactt
      241 gaccggccag accacgaggc ccccggcgtt gctcgaattt tccccgtccc tgagcgattc
      301 ccagaaatag ggaacgccaa gccagaccat gagaaggcag aatggcagga gaaaaacgac
      361 gtggccgaac aagtcgatcc agtcgcgggt gcgtttcgag agatggctgg ataaggcgtc
      421 gatgcggaca tgcccgttct tctgcagaac atacgcggcg gcaaacatga atacggcgcc
      481 aaagagggcg ccctgcagtt ccagccaggc attggaactc atgtcaaatg acttgcgaat
      541 tatggcgttg accgcgctga caaggaccgc cgccagaatg agccagcgca cctgcctgcc
      601 gatgaattcc gacagccggt caatgccgcc gctcaggcgc agcaaagtct ccacggttcc
      661 tcccttgacg ttctcccgtt gactgggatg attttggaac gaagcctaga tgagtttgca
      721 ggctccggca attattcaga gagcgggcag cgagagcagg ctgcgggctt ccgcaacgct
      781 ggcgggatgc cggccatagg accggcagag ttctgccacg cgtgcgacca gcgccgcgtt
      841 tgagggcgca agcccggccc catcccaccg gatattgtcc tccagccccg tacggcaatg
      901 accgccacgc tcaaggcacc aatgattgac ctcaagctgg tgcctgccga ttccggccgc
      961 tgtccaggtg gcctccggcg ccagccgcgc gatctcgtcc accatgaaat caaaggcggg
     1021 gcggatggcg ggcatggcat tcttcacccc gaaaacgaac tgcacatgca atggtttttg
     1081 cagcagccct tcctccgcca gcctcagcgc ctggtacagc atggaaaggt cgaatacctc
     1141 gatttccggc ttcaccttgt gcgccagcat ttttgcggcc agatcgcgga tgaggtccgg
     1201 ggaattctcg tagatgatgg tcggaaaatt gcacgagccc gtggccagtg aagccatgtc
     1261 acagccgaga tgcagcatgc ctccccgctc ggaacccttg ccggagcgcc cgcccgtgga
     1321 aaactgcacg atcagtcccg ggcaggcttt ccgcaatccc gccagcaggg cggcaaagcg
     1381 ctccgggtcg gaggtgggcg tctggtcgtc ctttcgcaca tggacatggg tgatgctcgc
     1441 ccccgcctca aaggcggcgc gggtcgagtc cacctgctca tcgatggtga tcggaaccgc
     1501 cggattatgc tccttgcgcg gcaccgaacc agttatcgca acggtaatta tgcacggcgt
     1561 tgtaatcact gcagtggcac gcaccggccc ttcttgtcgg gcgccatccc cttcgggcag
     1621 cgcttctgcg tgttctgctg gttctgctgg ttcagaaggt tgttgttgag atcgattttc
     1681 ggcagccgga gcgtgggctg gtcatcgtcc tgcggctcaa actccggctc ccgttcaatc
     1741 accacccgga cacagcgctg gcgcttgcgg tcgaatacgg ttccttccgg gcaagcctta
     1801 ggaatgttga ggcggatcgg ttcgcattcg ccggtgttgc ggttaagggc aaagccccgc
     1861 ggacagccct gctcctctgg gggttgctca acgatgcgct ggcactttcc gttcttcagt
     1921 ttgatgaagc ccttgggaca ggtggtctcc actcttggtg gcggctcgca cttcttggtc
     1981 ttcggattat actcaaggcc ctgcttgcat ttaaccgttg gtggcggcgg cggcggaacg
     2041 cgctcgcact catcggtctg cgggtttagc ctggtgcctt ccgggcattc cggttcatct
     2101 tgcgggatgc gctcacactg ctcgggattg ttgggattgc ggacaagacc ctgcgggcaa
     2161 ggcgcatggc atttgccccg gatcgtctca taaccttccg ggcaggatgg cgggctgcaa
     2221 accccgcgca ggttcggata actgccatcc tcgcagcgcg gcggcaggca gtacccataa
     2281 gagttgcgcg ttgtgccctg ctcgcaaacc ggcacgcatt gctggcggaa gttttcaaac
     2341 ccgaaggggc aggtttccgg cggcggcagg cagcgggtgg gatgatagat gtcgcgtgtg
     2401 aaaccttccg ggcacgatgg caggcagatc cacctgtagt catccggcca gtcgccgccg
     2461 ggtatgcagg cgccggtccg cgcattgcgc tggtagccct ccgggcaggc cttgtcatag
     2521 tagctgcggc acaggccgtt ttcggccaga taggttccgt ccgggcaatt tggcgagcac
     2581 cgcccggtct cttcggaata gctcaggcct tccgggcaat tgtcgggaat ttgaattctt
     2641 ctgtcaggca ggcattcatc tggattgctg aggttgcggt gatagccttc cggacagctc
     2701 ggcagcggca catggtcata gccttcggga cagccatgat cgcacggcgg caggcaaagg
     2761 ccgggatagc cgggataccg caaatagcca cccgggcatt ccggcaggca atagtcatcg
     2821 tagatcgtgt aacctgccgg acagcgcggc cccgagtcgc agctaccaca ggtgttgcaa
     2881 caggatttgg tctgcacttc acaggtttgc ggcacagatg cttgctgggc ccgtggcgcg
     2941 gtcgggagga ggactgcaat gagaactgca aagcaggcga tcaggaacga ccggtaaaat
     3001 gtggactgca agcagagctt cgcatgtgtc atggcgcacc atccccaact ttcccgcctt
     3061 gggagatgta ccgcatcaac ccgtaggcta gccccttgcc acggagatta cggccaatgg
     3121 aactgcgttg gcaaggggct ggacgttccg ccgcatatgg ctaagctcga taaacaggaa
     3181 cttccatgcg ccatgcccaa ctgacaagcc ttgacctgcc ggatttcggc agccccatga
     3241 ccgagccgga actgcaccgt gacatctatg cggcgcgcct caagcaactg ttcgcgcgga
     3301 tggctgccag cagtctcgac gcgttggtta tctatggtga ccgcgagcat gcagccaaca
     3361 tctcctgggc cacgggttat gatccacgct tcgaggaagc gatctgcatc atcgtgccgg
     3421 gccgcgcgcc cacccttctt gccggcaatg aaggctttcc ctatgccgaa atggcgatcg
     3481 gcagtttcga tcgcgtcttg tggcagccgc tgagcctcat gggccagccc cgcggcaaat
     3541 accgcgatct cgccagcgtc ctgcgcgagt ccggcatgaa gaaagacatg cgcatcggcc
     3601 ttgccggatg gaaaggcttt gaaactgacg acggtgtttt cgatccgcac tggttcgaaa
     3661 ccccgcatta tctcgtcgaa gcgctaaacg gcttcggcac tgtcaccaac gccgccctgc
     3721 tcttcatgca tcccaccgat ggcctgcgtg tgatcaatga agtggaacaa ttggcccgct
     3781 tcgaatatgc cgccaccctc gcctcaaatg ccgtccgccg cctgatcctc ggcgcgaaac
     3841 ccggcatgag cgaatacgcc gcctgccgca acatggcctt tgacggcttc ccgcaatccg
     3901 tccacgtcaa tatgtcatca ggtccccgcg cgaaatacgg cccgcccagc ccatcgatgc
     3961 gggtcattga aaaaggcgat cccatcgtca ttggcgtcgg cctcatgggc gccctgaatt
     4021 gccgcgccgg gttcatggtg gccgaggcca gcgaactgcc cgcgaatatc cgcgactacg
     4081 tcgacaagct ggtggccccc tatttcgccg ccgccgcggc ctggtacgaa accatcggca
     4141 tcggtgtcga aggcggcgtg ctctacgaag cggtgatgag ccgtttggcc gaccccttct
     4201 tcggcatcgg gctgaacccc ggccatctca tccacctcga tgaatgggtc cattccccca
     4261 taaaacagaa tagcaggatg aggctcgcct ccggcatggc gctccaatgc gatatcattc
     4321 cggcaaccgg cacgccctat ttcatgtcca acatcgagga tggcgtggca ctggccgatg
     4381 ccccaacccg cgccgccttc caggcaaaat tccccgaagc ctggaaacgg attgaaagcc
     4441 gccgcgcttt catgacagaa aagctcggca tcaccttgaa acccgaagta ctgcccttca
     4501 gcaatattcc cgcctggctg ccgcccttct ggctgtcgcc gggcaaggtc atggcgatgc
     4561 gctgattact ccccgtaggg aatccagatg ttcttcacct gcaccgcatg gcgcagcagc
     4621 accgggccct cgctcgaggc cgaatcgaac cagtccgtgg ccaggccatg gtcaaccagc
     4681 gtgcgcttga gattgccggc agagagcttt tcggcgctgg ccgaattctc cttcgagccg
     4741 aacacccaca ggccatccac atcatcatgt tcggcaagcg tcttgagcaa cgtatcgcgg
     4801 ccacccgtta cgatgttgat gacgcctgcg ggcacgtccg aagtttcaag aacctgatag
     4861 aaatcggttg ccgccagggg atgtctttcg cttggcacta ccaccgcgcg gttgcccatc
     4921 gcaataagtg gcgccaccag gcttatgaaa ccaagcagcg gcgcttcatc cgggcagatc
     4981 acgccgacaa cgcccagcgg ctcattcatc gccagtgcca cgccgcgcag aggcggtgaa
     5041 tgaacggcac catcatactt gtcggcccag gcgccatagc tgaacaaccg caggatcgat
     5101 gccttgactt cagattttgc tttcgaaacg cctgtcatcg ccttgatgcg a
//
LOCUS       DAORVN010000042         5145 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_11241_length_5145_cov_2.638900, whole genome shotgun sequence.
ACCESSION   DAORVN010000042 DAORVN010000000
VERSION     DAORVN010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 5145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5145
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11241_length_5145_cov_2.638900"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="PKV23_01390"
     CDS             <1..222
                     /locus_tag="PKV23_01390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX84114.1"
                     /translation="IENQVLGLQIMKERSSAMTYNWFTSRFTINRQKIDNNEVSSHRE
                     PLDHPELARMTLRELADLPMPHYTLQNCN"
     gene            complement(289..1719)
                     /locus_tag="PKV23_01395"
     CDS             complement(289..1719)
                     /locus_tag="PKV23_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018182476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HQX84115.1"
                     /translation="MSNNLKFYIDGAWVDPAVPNSLGVVNPSNEEVFAHISLGSKADV
                     DKAVAAAKRAFKTFSVTSRAERLDLLKRIVEQYKKRSKDLALAVSQEMGAPLPFALDS
                     QVGVGALHLEKMIEVLKTYQFEQAKGKSLVVREPIGVVGLITPWNWPLNQITCKVGPA
                     LAAGCTMVLKPSEIAPLDAIIFAEILHEAGVPKGVFNLVNGDGPTVGQSLAQHPDVDM
                     MSFTGSTRAGILVAKSGADTVKRIHQELGGKSANILFPDVDLELAVTKGVAGCFGNSG
                     QSCNAPTRMYVPRERHDEAAAIAKTAAEKFSVGPADDPKSRLGPVVSQIQFDKIQDLI
                     KSGIDEGAALVTGGTGRPGGLNRGYYVKPTIFANVSQDMRIAREEIFGPVLAIMPYET
                     IDEVVDRANDTVYGLASYLQSKDQKRAQEVARRMRSGNVYINYPAWDAGMPFGGYKQS
                     GNGREYAEYGLEDFMEIKGIVGYEAA"
     gene            complement(1986..>2467)
                     /locus_tag="PKV23_01400"
     CDS             complement(1986..>2467)
                     /locus_tag="PKV23_01400"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012254310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HQX84116.1"
                     /translation="LRGVIGHDVVLLLVYGTLVFLVGLSLTLLVMLPIYFIATWIGGW
                     LFFVQHQFEETHWEYADEWDMKIAALKGSSFLVMPPVLNWFSCNIALHHIHHLCSRIP
                     NYRLQECLKGNPELETVSPRLSIWQALKASRLALWDESSKRLISFREFGRKQELQAT"
     assembly_gap    2468..2477
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2478..3053)
                     /locus_tag="PKV23_01405"
     CDS             complement(<2478..3053)
                     /locus_tag="PKV23_01405"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012254310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HQX84117.1"
                     /translation="MDIQNETQYSAELRLQAKRLTAHCNLYRDASSRKAIIQLITTAV
                     PFFTLIAVMFLVAPHAYWLTLLLAIPTGALLTRFFALQHDCGHGSFFPSRRTNEMVGR
                     LISVLTFTPYDHWRRSHALHHQLSGNLDHRGFGDIETMTVKEYSQLGAWGRFRYRLYR
                     NPLIILVLGPPIFFLLLQRLAIGSNMPLRDSL"
     gene            complement(3154..4290)
                     /locus_tag="PKV23_01410"
     CDS             complement(3154..4290)
                     /locus_tag="PKV23_01410"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013531181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HQX84118.1"
                     /translation="MSSFDVIIIGAGAAGLMCAISAGSRGKRVLLLDHNAQAGAKILI
                     SGGGRCNFTNTGTSPENFLSKNPHFCRSALSRYTPADFLALMARHNIAWHEKTLGQLF
                     CDGSARQVVAMLLAECAAAGVVVKLATKVTEIRKSDGFIVDTATETFTAPALVLATGG
                     LSIPKMGATGFSYDVAKRFGLKVITPRPALVPLKAQPDELEFYKGLSGVSLPAIATCG
                     AASFRENILFTHRGLSGPAILQISSYWREGQSIHLDLAPGIDALTFLKERRHARPNAE
                     LKTILAELLPQRLAQAMAPGDLNQLAALLKNWTMTPETSEGFAKAEVTAGGIDTDQLS
                     SKTMEAKAVSGLYAIGEAVDVTGWLGGYNFQWAWASGWCAGQAV"
     gene            complement(4343..>5145)
                     /locus_tag="PKV23_01415"
     CDS             complement(4343..>5145)
                     /locus_tag="PKV23_01415"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HQX84119.1"
                     /translation="KVNAEEANKAKSIFLATMSHELRTPLNAIMGFSEILKRELFGPL
                     SVPAYKDYAGDIHHSGGYLLDLINDILDLSRIEAGRREIQEEPFDLLACVKSAQALLN
                     GKASEKSVAVKVEITPSLPKLMGDLRGVNQILINLLTNAIKFTPAGGQVVVSAARNAS
                     GGMVLSVKDNGPGIPAHEVQSVLNSFSRGSLATKGAVDGAGLGLSIVKGLMDLHGGDV
                     SIVSEVGKGTEVLITFPAHRVLTGPRGEIIAAPNVQSESQRKLIAITG"
BASE COUNT         1054 a         1473 c         1553 g         1055 t
ORIGIN      
        1 atagaaaacc aggtgctggg gttgcaaatt atgaaagaaa ggagttcggc catgacctat
       61 aattggttca catcaaggtt taccattaac cgccaaaaga ttgataataa tgaagtttca
      121 agtcatcgcg agccgctcga tcatcccgaa ctcgcacgca tgacgctacg ggaactggca
      181 gacctgccca tgccccatta tacgctccaa aactgcaatt aagctgatgc accaagccca
      241 tcggccagca cttggggcgc tggccgtggc cgtctgtatg agaactcctc aggcggcttc
      301 gtagccgacg atgcctttta tctccatgaa gtcctccagc ccgtattcgg cgtattcgcg
      361 gccattgcct gactgcttgt agccgccgaa gggcatgccc gcatcccagg ccgggtagtt
      421 gatatagacg ttgccgctgc gcatccgccg ggcaacctcc tgggcccgtt tctggtcctt
      481 gctctggaga taggaggcga gcccgtagac cgtgtcattg gcccggtcca ccacctcgtc
      541 gatggtttca tagggcatga tggcgagcac cggcccgaaa atctcttcgc gcgcgatacg
      601 catgtcttgg gagacattgg caaatattgt gggcttcaca tagtagcccc ggttaagccc
      661 gcccggccga ccggttccgc cggttacaag ggccgcacct tcgtcgatgc cgctcttgat
      721 caggtcctga atcttgtcga actggatctg gctgactacc ggcccaagcc ttgatttcgg
      781 gtcgtccgcc gggcccacgc tgaatttctc ggccgccgtc ttggcgatgg cggccgcctc
      841 atcatggcgt tcgcggggca catacatgcg tgttggcgcg ttgcaggatt ggccgctgtt
      901 gccaaaacag ccggcaacac ccttggtgac ggccagttca aggtccacat cgggaaaaag
      961 gatgttggcg gatttgccgc caagttcttg atgaatccgt ttcactgtat cggcaccgga
     1021 ctttgccact agtatgcccg cccgggtcga gcccgtaaac gacatcatgt cgacatcggg
     1081 atgctgggcc agggattggc caaccgtcgg gccatcgcca ttcaccaggt tgaacacgcc
     1141 cttgggaacc cctgcctcat gcaggatttc agcaaagatg atagcatcca gcggggcgat
     1201 ctcgcttggc ttcagaacca tcgtgcagcc cgccgcaagc gccgggccca ccttgcaggt
     1261 gatctggttg agcggccagt tccacggcgt gataaggcca actactccga tgggctcgcg
     1321 gaccaccagc gatttgccct tggcctgctc gaactgatag gtctttagca cctcgatcat
     1381 tttctcgaga tgcagggccc cgacaccgac ttgtgaatcg agcgcaaagg gcaggggtgc
     1441 ccccatttcc tgggaaaccg ccagggcaag gtccttgctg cgctttttgt attgttccac
     1501 gatgcgcttg agcagatcga ggcgttcggc ccggctggtg acgctgaagg tcttgaaggc
     1561 gcgcttggcc gctgctaccg ccttgtccac gtcggccttg gagcccagcg aaatatgggc
     1621 aaaaacttcc tcgttggaag gattgacgac acccagactg ttgggcacag ccgggtcaac
     1681 ccaggcgcca tcgatataga atttgaggtt gtttgacatg tgcttcactc ctgatttctg
     1741 atagatggtt cacgtcactc tgttcagtct gctccagcgg cgcccgcctt cagggtgggg
     1801 ccgcgattat ttgcattttt ggggttgcat ccagccaggt tgcttttggc aaagagcaaa
     1861 aggcatccat tgcctcttcg atgtatccat atgaacataa cgcattgcgg tcagcaacag
     1921 cctgggatgc atgaggcccg tgcatttatg ttgatgatct ccctgtgccg ggggtatccg
     1981 cgcgcttaag tcgcttgcag ttcttgcttc cgcccaaact cgcggaagga aatcagccgt
     2041 ttggaggact catcccacag ggccagccgc gaagccttca gcgcctgcca gatcgacagc
     2101 cgcggcgaaa ccgtttccag ctccgggttg cctttcaggc actcctgcaa acggtaattc
     2161 gggatgcggc tgcagaggtg atgaatatgg tgaagggcga tgttacaact gaaccagttc
     2221 agcacaggcg gcatcaccag gaaggaactg cccttcaggg cggcgatctt catatcccat
     2281 tcgtcagcat attcccaatg ggtttcctcg aactggtgct ggacgaaaaa tagccagccg
     2341 ccaatccagg tggcgatgaa atagatgggc agcatcacca gcagggtgag cgacaggcca
     2401 accaggaata ccagcgtgcc atagaccagc agcagcacca cgtcatggcc gataacgccg
     2461 cgcaggcnnn nnnnnnncag gctgtcgcgc agcggcatgt tggagccaat ggcaaggcgc
     2521 tgcaaaagaa ggaaaaagat cggcgggcca agcacaagga tgatcagcgg attgcggtag
     2581 agccggtagc ggaaacgccc ccaggcgccc aactggctgt attccttgac cgtcatggtc
     2641 tcaatgtcgc caaagccgcg gtgatccaga tttccggaaa gctgatgatg cagcgcatgc
     2701 gagcgccgcc aatgatcgta aggggtaaag gtcagcacac tgatcagccg cccgaccatt
     2761 tcgttggtgc gccgggacgg aaagaacgag ccatgaccgc agtcatgctg gagcgcgaag
     2821 aaacgcgtga gcaaggcacc ggtgggaatg gcaagcagca gcgtgagcca gtaggcgtgc
     2881 ggcgccacca ggaacatgac ggcgatcagc gtgaaaaacg gaacggcggt ggtgatcagc
     2941 tggatgatgg ctttgcggct gctggcatcg cggtagagat tgcagtgcgc ggtcaaccgt
     3001 tttgcctgca ggcgcagctc cgctgaatac tgcgtctcgt tttgaatatc caagagtttc
     3061 cccagtacgg atttgtgagt cctgaatctc cctttacatg gttcgcggcg ctgcgtctgt
     3121 atttattaca ttaaaattac gtcagatggc cgctcaaacc gcttgcccgg cgcaccagcc
     3181 gctggcccag gcccattgaa aattatagcc gccaagccag cccgtgacat caaccgcttc
     3241 gccgatcgca tagaggccgg aaaccgcctt tgcttccatg gtcttggagg aaagctgatc
     3301 tgtatcaata ccgccggctg tgacctccgc cttggcgaag ccttcggacg tctcaggcgt
     3361 catcgtccaa tttttgagca gggctgcaag ctggttcaaa tcaccgggcg ccatggcctg
     3421 agccagccgc tgcggcagga gttcggccag aatagtcttg agctcagcat tgggccgcgc
     3481 atgccgtcgt tccttgagaa aggtgagagc atcgatgccg ggcgcaaggt caaggtggat
     3541 cgattggccc tcgcgccagt aggatgaaat ctgcaggatg gccggaccgg aaaggccgcg
     3601 gtgggtgaag agaatgtttt cccggaacga cgccgcgccg cacgttgcaa tagccggaag
     3661 tgaaacgccg gatagaccct tgtaaaactc cagctcatcc ggttgggcct tcaaggggac
     3721 cagagccggc cgcggcgtga tcaccttgag gccaaatctt ttggccacat cgtaggaaaa
     3781 tccggttgcc cccattttgg gaatggagag ccccccggtg gccaggacaa gggccggagc
     3841 cgtgaaggtc tcggttgctg tatccacgat gaagccatcg gacttgcgga tttcggtgac
     3901 tttcgtggcg agcttcacca caacgcccgc tgcggcgcat tccgccagca gcatggccac
     3961 gacctgccgg gccgaaccat cacagaaaag ctggcccagg gttttttcgt gccacgcgat
     4021 gttgtgcctg gccatgagcg ctagaaaatc cgctggcgta taacggctga gcgcgctgcg
     4081 gcagaaatgc ggattctttg agaggaaatt ttccggcgag gtgccggtgt tggtgaagtt
     4141 gcagcgcccg cctccggaga tcaggatctt ggcgccggct tgggcattat ggtcgagcag
     4201 cagaacccgc ttgccccgcg acccggccga gatggcgcac atgaggcccg ccgcaccggc
     4261 gccgataatg atgacatcaa aggacgacat ggcggctggc tagcatgcca gatcggcctg
     4321 cggtactgtt ttcaggcgaa acctatccgg tgattgcgat cagcttgcgc tggctttccg
     4381 attggacatt tggcgccgcg atgatttcgc cgcgcggccc cgtgagtaca cggtgggccg
     4441 ggaaggtgat cagaacctcg gtgcccttgc ccacctcgct cacgatgctg acgtcgccgc
     4501 catgcaggtc catcaggccc ttcacgatgg acaggccgag gcctgccccg tccacggcgc
     4561 ccttcgtggc gagcgagccg cgcgagaatg aattgagcac cgactggacc tcgtgggccg
     4621 gaatgccggg accattgtcc ttgaccgaca ggaccatgcc gcctgaggca ttgcgcgccg
     4681 ccgagactac aacctggccg ccggcggggg taaacttgat ggcattggtc agaaggttga
     4741 tcaggatctg gttgacgccg cgcaagtcgc ccatcagctt cggcagcgat ggcgtgattt
     4801 caaccttgac ggcgaccgat ttctcgctgg ccttgccatt gagcagggcc tgggcggatt
     4861 tcacgcaggc caggaggtcg aacggttctt cctggatttc gcgccgtccc gcctcgatgc
     4921 gcgacaggtc gaggatgtcg ttgataaggt cgaggagata gccgccggag tgatggatgt
     4981 cgccggcata atccttgtag gcagggaccg agagggggcc aaacagctcg cgtttgagga
     5041 tttcggaaaa tcccatgatg gcgttcagcg gggtgcgcag ttcatggctc atggtggcga
     5101 ggaaaatgga tttcgccttg ttggcttcct cggcattgac ctttt
//
LOCUS       DAORVN010000043         5103 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_11400_length_5103_cov_3.678883, whole genome shotgun sequence.
ACCESSION   DAORVN010000043 DAORVN010000000
VERSION     DAORVN010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 5103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5103
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11400_length_5103_cov_3.678883"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /locus_tag="PKV23_01420"
     CDS             complement(<1..226)
                     /locus_tag="PKV23_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004111453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HQX84120.1"
                     /translation="MAVLLIAEHDNKSLKDATHKALTAAAQMGGDVHVLVAGNNAAAA
                     AAQAAKLTGVKKVLHAESPSLERPLAEPMAA"
     gene            complement(229..975)
                     /locus_tag="PKV23_01425"
     CDS             complement(229..975)
                     /locus_tag="PKV23_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010657517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HQX84121.1"
                     /translation="MKVLVAVKRVVDYNVKIRVRADGSGVELANVKMSMNPFDEIGVE
                     EAVRLKEAGKATEVVAVTIGPAQAQETLRTALAMGADRAILVKNDEYVEPLGVAKILK
                     GVVEAEKPDIVILGKQAIDDDCNQTGQMLSALLGWAQATACSKLEPGAGEATITREID
                     GGLQTLKVKMPLIVTTDLRLNQPRYASLPNIMKAKKKPLDEKTPADYGADVKPRLKVL
                     KTDEPPKRLAGVKVKSVAELVSKLKEAGAI"
     gene            complement(1073..1765)
                     /locus_tag="PKV23_01430"
     CDS             complement(1073..1765)
                     /locus_tag="PKV23_01430"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HQX84122.1"
                     /translation="MFVPLHDDTPLKVIRFQFVTIAIIAGNILVFLMTGAFQSETMLA
                     GVATSFGVVPSDLTTAFSQNIPYSPIPEPLTFLTYMFLHAGWLHLISNMLFLWVFADN
                     IEDAFGYFAFVLFYLLCGIAGALAHVLMSPASTAPLIGASAAVSGVIGAYMLLYPRAR
                     VWILLFFRIPLRISAIWVLGGWFLLQIFSVLTTDQSAEVEVAWWAHIGGFVAGLAITF
                     LLRSRLLVRTSR"
     gene            complement(1765..2349)
                     /locus_tag="PKV23_01435"
     CDS             complement(1765..2349)
                     /locus_tag="PKV23_01435"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010973170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HQX84123.1"
                     /translation="MVVLNRIYTRTGDKGDTALGSGTRVSKAHLRIQAYGTVDETNAV
                     VGIVRLQTSTEAFAKLDAMLARIQNELFDLGADLCVPDTGEKLTYEPLRILPKQVERL
                     ESEIDDLNHDLDPLRSFVLPGGHVAAAHLHLARTICRRAERLIVELKSHQGEHVSEPA
                     LTYINRLSDFFFVASRWANAKTTGDILWVPGKTR"
     gene            complement(2382..4904)
                     /locus_tag="PKV23_01440"
     CDS             complement(2382..4904)
                     /locus_tag="PKV23_01440"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HQX84124.1"
                     /translation="MNVALWLRFAARDLRSGLQGFWIFLTCLTLGTAAIAIIGSLSAS
                     IERGLDEQGQPLLGGDVEFSLIHREATQAELNFIASNGTVSKVATLRAMAIAPDTTTL
                     VEIKATDTAYPLYGRIILENNGSLQNATAFQDGAHGVAADPLLLGRLGVKLGDSIKVG
                     TLEVAIRGIITTEPDRISDGFILGPRLLMSEDALKATGLIQPGSLVTYRYRVKLENDQ
                     SLQAARNLVTKAREQFPDAGWQLRSRDRAAQGAENFVERLSYFLTLVGLTSLIVGGAG
                     IANAVSAFVDRRIGTIATLKCLGAPARDVFAIYLTEILAVAFLGIAIGLAAGAATPII
                     AHTFLQNFLPLPLTTQIELAPLALTAALGLLVTIAFSIWPLARTHRIPASALFRHRIV
                     HLYGFPRWPYLASIAAALACIAALIFLSFENQRATAYYLGGLLASFVILLGLAFAIIK
                     LAEKIPKPRNAIWRYAIANLYRPGSSAVSVILALGLGLTLFVTLALTDQTISKELRAG
                     IPEKAPAFFFLDVRNEQLPAFAEAAKKFDGVKDVANAPMLRGRIARIGDTPADKVRSQ
                     GDSGWALRGDRGLTYSEALPEGSVLVQGEWWEKDYNGPPLVSFVSGVAQDIGLKIGDS
                     VTVNVLGREVTAKVANFRDVNWRTMGINFVMVFSPNTLKSAPHSHVVTIEMEGGDEAR
                     LLNGMAREFPSVTAVRVKEALATVSELLGKMLGAIRGANALTLLTGILVLAGALAAGL
                     SNRIYDAVVLKTYGASRAQLMGAFIIEYAILGLAAAIFGVAVGSLGSWFLATWILDMP
                     WSFSFIVAATTALLAMLITIAAGLLVTWAALAAKPAPILRNE"
     gene            complement(4901..>5103)
                     /locus_tag="PKV23_01445"
     CDS             complement(4901..>5103)
                     /locus_tag="PKV23_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012110288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX84125.1"
                     /translation="ARILLADEPTGNLDQETGEQIIKLLFELKEQESASLLLITHDRA
                     LAKRCDRIAEVRDGKVISGNHP"
BASE COUNT         1065 a         1376 c         1675 g          987 t
ORIGIN      
        1 tggcggccat gggctcggca agggggcgct ccagcgaagg gctttcggcg tgaaggactt
       61 ttttcacgcc cgtcaacttc gcagcttgag cggcggcggc ggcggcattg ttgccagcca
      121 ccagcacatg cacgtcgccg cccatctggg ctgcggcggt gagcgccttg tgggttgcat
      181 ccttgaggga cttgttgtcg tgttcggcaa tgagaagcac ggccatgatc agattgctcc
      241 tgcttctttg agtttggata ccagttcggc aacggatttg accttgacac cggcgaggcg
      301 ttttggcggc tcatcggttt tcaatacctt gagccgcggc ttcacatcgg cgccatagtc
      361 agccggggtt ttttcatcca gcggcttttt cttggccttc atgatgttgg gcagcgaggc
      421 atagcgcggc tggttgaggc gcaagtctgt cgtcacgatc agcggcatct tcaccttgag
      481 agtttgcagg ccgccatcga tttcgcgggt gatggtggct tcgccggcac cgggctcgag
      541 cttggagcag gcggtggcct gggcccagcc gaggagggcg gacaacattt ggccggtctg
      601 gttgcagtca tcgtcaatgg cctgcttgcc gagaatgacg atatcgggtt tttcggcttc
      661 gacgacgccc ttcaaaatct tggccacgcc cagtggctca acatactcat cgttcttcac
      721 cagaatggcg cggtcagccc ccatggcgag cgcggtgcgc agcgtttcct gcgcctgggc
      781 ggggccgatg gtgacggcca caacttccgt tgccttgccc gcttccttga ggcgcacggc
      841 ctcttcaacg ccgatctcat cgaaagggtt catggacatc ttcacgttgg ccagttcaac
      901 gccggagcca tcggcgcgga cgcggatttt cacgttgtaa tcgacaaccc gcttgacagc
      961 gaccagaacc ttcatcggca accccttgag aatttgcggc ggaacctagt gaaccaccta
     1021 ctccgagtca attctgccac cgacatgccc catctgcttg cgacgccttg cctcagcggg
     1081 aggtgcggac gagcagccgt gaacgcagca gaaaagtgat ggcaagtcct gccacgaagc
     1141 ccccgatatg ggcccaccag gcaacctcga cttcggcgga ctggtcggtt gtgagcacac
     1201 tgaagatctg cagcaggaac cagccgccca ggacccaaat ggccgaaatg cgcagcggaa
     1261 tgcggaagaa cagcagtatc cagacccggg ccctgggata gagcagcata taggcgccga
     1321 tcacaccgga cacggccgcc gacgccccaa tgagaggggc ggttgacgcg gggctcatca
     1381 gcacatgggc gagggctccg gcaatgccgc agagcaggta aaacagcaca aaggcaaaat
     1441 aaccaaaggc gtcctcgatg ttgtcggcga agacccagag gaacagcatg ttggaaatca
     1501 ggtgcagcca gcccgcgtgg agaaacatgt aggtaagaaa ggtcaggggt tcggggatgg
     1561 ggctgtatgg aatgttctgg gaaaaggcag tggtgagatc ggagggcaca acgccaaaac
     1621 tggtggcaac gcccgccaac atggtttcgg actggaaggc gccagtcatg aggaagacga
     1681 gtatgttgcc cgcgatgatt gcgatcgtaa caaactggaa gcggatgacc ttcaggggtg
     1741 tatcgtcatg gagggggacg aacactacct ggtttttccc ggtacccaga ggatgtcgcc
     1801 ggtggtcttg gcattggccc agcggctggc gacaaagaag aagtcggaga ggcggttgat
     1861 ataggtcagc gctggttcgc tgacatgctc tccctgatgg gacttgagct caacaatcag
     1921 gcgttcggcg cggcggcaga tggtgcgcgc cagatggaga tgggccgccg ccacgtggcc
     1981 gccgggcagg acaaacgagc gcaatgggtc gaggtcgtgg ttgaggtcat ctatctcgct
     2041 ttccaggcgc tccacctgct ttggcaatat gcgcaaaggt tcgtaggtca gtttctcgcc
     2101 ggtatcggga acgcagaggt cggcgccgag atcaaagagt tcgttctgaa tgcgagccag
     2161 catggcatcg agtttggcaa aagcctcagt gctggtttga agtctgacaa ttcccacaac
     2221 cgcgttggtt tcatcaaccg tgccataggc ctgaatgcgg agatgggcct tggaaacgcg
     2281 ggtacccgag cccaaggccg tgtcgccctt gtcgccggtg cgggtgtaga tgcggttgag
     2341 tacgaccatg gcctctgagt agcgtgaacc gcccccaatt tctactcgtt tcttagaatc
     2401 ggcgcgggct ttgcggcgag tgctgcccag gtcaccagaa gaccggcggc aatggtgatg
     2461 agcatggcga gaagagccgt ggtggcggcc acgatgaagg agaagctcca cggcatgtcc
     2521 agaatccagg tggcaaggaa ccaggagccg agcgagccta cggcaactcc aaaaatggcg
     2581 gcggcgagcc ccagtatggc atattcgatg atgaaggcgc ccatgagctg ggcccgcgag
     2641 gcgccatagg ttttcaggac caccgcgtca taaatgcggt ttgaaagtcc ggcggcaagt
     2701 gctccggcga ggaccagaat tccggtcagc aaggtcaggg cgttggcacc gcggatggcg
     2761 ccgagcattt ttccgagaag ttcgctgacc gtcgccagag cttccttgac gcgcacggcg
     2821 gtaacggagg gaaattcgcg cgccatgccg ttcagcagcc ttgcttcgtc gccgccttcc
     2881 atctcaatgg tgacgacatg gctgtggggg gcggacttca aagtgttggg tgaaaacacc
     2941 atgacgaagt tgatgcccat ggtacgccaa ttcacatcgc ggaagttggc cacctttgcg
     3001 gtgacctcgc ggcccagcac attcacggtg acactgtcac cgattttcaa accgatgtcc
     3061 tgggcaactc cgctgacaaa cgagacaagg ggcgggccgt tataatcttt ctcccaccac
     3121 tccccttgca ccagcacgga gccttcaggc aaggcttcgg aataggtgag gccgcggtcg
     3181 ccgcggagcg cccagccgct gtcaccctga gaccgtacct tatcggcggg ggtatcaccg
     3241 atgcgggcga tgcggccgcg cagcatgggg gcgttggcaa cgtccttaac gccgtcgaat
     3301 ttcttcgcgg cttcggcaaa ggcgggaagc tgctcattcc tgacatcgag gaagaaaaag
     3361 gcaggagcct tttccggaat gccggcgcgc aattccttgc tgatggtttg gtcggtcagg
     3421 gcgagggtaa caaagagggt aaggcccagg ccgagcgcga ggatgacgga aaccgccgat
     3481 gaaccggggc ggtaaaggtt ggcgatggcg tagcgccaga tcgcgtttct gggctttgga
     3541 attttttctg cgagcttgat gatggcaaag gcgaggccca gtaaaatcac gaagctcgcc
     3601 agcagaccgc caaggtaata ggcggtggcg cgctggtttt cgaaacttag aaatatcaag
     3661 gcggcgatac aggcgagggc tgccgcaatg cttgcgagat agggccagcg gggaaagcca
     3721 tagagatgca cgatgcggtg gcggaacagg gcggaggccg gaatccggtg ggttcgggca
     3781 agaggccaga ttgaaaaggc aatggtcact agcaggccaa gggctgccgt caaagccagg
     3841 ggcgcaagtt cgatttgcgt tgtgaggggt aacggcagaa aattctgcag gaaggtatgg
     3901 gcgataatag gggttgcagc ccccgctgcg aggccgatgg cgatgccgag aaaagccaca
     3961 gccaggattt ccgtgagata gatggcgaag acatcccggg caggtgcgcc caggcatttc
     4021 agggttgcga tggtgcctat gcggcgatca acaaaggcgg acaccgcgtt ggctatgccg
     4081 gcgccgccga caatgagcga ggtcagtccc acgagcgtga ggaaatagga aagccgctcg
     4141 acaaaatttt ccgccccctg ggcggcccgg tcgcgggagc gcaattgcca gccggcatcg
     4201 ggaaactgct ctctcgcttt cgtgacgaga ttacgggcgg cctggaggct ctgatcattt
     4261 tcaagtttca cgcggtagcg ataggtgacg aggctgccgg gctggatgag gccggttgcc
     4321 ttaagcgcat cttcgctcat cagcaggcgt gggcccagga tgaaaccgtc ggatatgcga
     4381 tcgggctcgg tggtaatgat gccacgtatg gcaacctcga gcgtgccgac tttgatgctg
     4441 tcgcccagct ttacgccgag ccgtcccaga agcagaggat ctgcagccac accgtgagcg
     4501 ccatcctgaa aggcggttgc attttgcagc gagccgttgt tttcgagaat gatcctgccg
     4561 taaagcggat aggcggtatc ggttgccttt atctcgacca gtgttgttgt gtcgggggct
     4621 atggccatgg cgcgcagtgt cgccactttg ctgacagtgc cgttgcttgc aatgaagttc
     4681 agttcggctt gggttgcttc gcggtggata agggaaaact cgacatcgcc gccgagcagg
     4741 ggctggccct gttcgtcgag cccccgttca atggaggctg agagggagcc gataatggcg
     4801 atggcggccg tacctaaagt gaggcaggtg agaaaaatcc agaaaccctg caggcctgaa
     4861 cgcaagtctc gggcggcgaa acggagccaa agcgcgacat tcatggatga ttgccggaaa
     4921 tgaccttgcc gtcgcgcact tcggcgatgc ggtcacagcg cttggccagg gcgcggtcat
     4981 gggtgatcaa cagcagcgag gcactttcct gctctttcag ttcgaagagc agcttgatga
     5041 tctgttcgcc ggtttcctga tcgagatttc cggtgggttc atcggcaagc aggatccggg
     5101 cac
//
LOCUS       DAORVN010000044         5067 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_11552_length_5067_cov_2.869912, whole genome shotgun sequence.
ACCESSION   DAORVN010000044 DAORVN010000000
VERSION     DAORVN010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 5067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5067
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11552_length_5067_cov_2.869912"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..333)
                     /gene="ssb"
                     /locus_tag="PKV23_01450"
     CDS             complement(<1..333)
                     /gene="ssb"
                     /locus_tag="PKV23_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018184510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HQX84126.1"
                     /translation="MAGSVNKVILVGNLGKDPEVRHTQDGKAIVNLSLATSENWRDKA
                     TGERKERTEWHRVVIFNENLAKIAEQYLKKGSTVYVEGALQTRKWTDKDGIEKNSTEV
                     VLQNFRGEL"
     gene            complement(484..627)
                     /locus_tag="PKV23_01455"
     CDS             complement(484..627)
                     /locus_tag="PKV23_01455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX84127.1"
                     /translation="MNWVFGAYSNVYSTAMMQNKDAVAYLANAKAGEIKSTGRIARLF
                     GRA"
     gene            complement(724..861)
                     /locus_tag="PKV23_01460"
     CDS             complement(724..861)
                     /locus_tag="PKV23_01460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX84128.1"
                     /translation="MNWIFEAYSNVYNTAMGQRTLHHQPGKPAAEQKQRLKRAHGKHD
                     L"
     gene            968..1942
                     /gene="gcvA"
                     /locus_tag="PKV23_01465"
     CDS             968..1942
                     /gene="gcvA"
                     /locus_tag="PKV23_01465"
                     /inference="COORDINATES: protein motif:HMM:NF008352.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GcvA"
                     /protein_id="HQX84129.1"
                     /translation="MARRLPSLNALKAFEAAARHVSFTEAAGELFVTHAAISRHIREL
                     EEYLGTQLFLRTGRGVALTEAGKRYGEQLTPLFDALSNASREAAAVGAARPLNVSVEP
                     AIASRWLVPRLGHFKELHPDIELNIDPTTRLVDFRVDEVDLGIRYGRGNWEDVEMTKL
                     SSNVVIFPVCSPALIAGVENLKPEDLKNFNLLHEQRKQWWAEWLNANGIEGVTDWKGT
                     VFQNHLAIEAAESGQGFALGDQILCTDSIVEGWLARPFALEMKDHGNYWIVRAKGSKE
                     TATARSFREWLTSEMAETNKKYAALKASSKPAKQAIAPAGLFYPQSNH"
     gene            1976..2353
                     /locus_tag="PKV23_01470"
     CDS             1976..2353
                     /locus_tag="PKV23_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HQX84130.1"
                     /translation="MARKLYDCMKCPGYCCSYPVIEVKDRDAERIARHFALPLAKVEK
                     KFFKKAHGYKRIMRRKKDEIYGKICQFFNTTERRCTIYHARPSTCRAFPGDGHCGYYD
                     FLKFERDGQEDENYISTTNHSGN"
     gene            2396..3721
                     /gene="hisD"
                     /locus_tag="PKV23_01475"
     CDS             2396..3721
                     /gene="hisD"
                     /locus_tag="PKV23_01475"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006468851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HQX84131.1"
                     /translation="MTSHINYLVLSDLTAADRATLLRRTESDLKPFIEKVKPIIDAVR
                     TEGDRALSRFTEKFDGALVEESAIAATPEDFDRAHRELEADVREAIVFAAANIRKFHE
                     LQKPEASWSAETRPGLHAGDRTTPIASVACYIPRGKGSFPSSVLMTCIPASVAGVEDI
                     CIVTPPGPDGRIDAASLVAARAAGVSKVYKVGGAQAIAAVAYGTKTISRYVKVVGPGS
                     PWVVAAKRLLADQIDTGTPAGPSEAIIFADETTDGRLAALDLLIEAEHGDDSSAFIVT
                     TSRDVADAALAAIPTYWHRMSAQRVNFTKAVLISSRGGILLAKDAAEAMAFINDYAPE
                     HLQILSKEPRKYLPMIKNAGEILLGEHTPSTLGNFVLGPSHVLPTSGWAKTFSALSVH
                     DFMKRTSIVEVTAQAYPELARHAAVIARYEGFDAHANAVSDLRKKLLGN"
     gene            4148..5065
                     /locus_tag="PKV23_01480"
     CDS             4148..5065
                     /locus_tag="PKV23_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018323964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HQX84132.1"
                     /translation="MNTATHSIPLADHAGSRRRGILLVAGSAIVWSFGGAIARFLEVD
                     NSWTIVFWRSLFAALFLLGFMLWRDGPRGTVALFKGMGLAGLGVALCFAIASSSFVVA
                     LAYTTVANILLMQAGVPLIAALLTWTLFRERISGATWAAIAAVIAGVAIMVSESFTGK
                     VSPIGDGLALLISVMFAIATVITRRHAHVRMTPAVCLGTILAACFAGLLAGHYAVSAA
                     DLGWLVTFGALNLGLGLVMFTTGVRLLPAALAALVGTLEPVLGPIWVWLVHNEIPSGR
                     TLLGGAVVFTALVIHLLIDWQRQSRAQSR"
BASE COUNT         1013 a         1626 c         1309 g         1119 t
ORIGIN      
        1 caattcaccc cggaagttct gcaggaccac ttcggtcgaa tttttctcaa tcccgtcctt
       61 gtccgtccac ttccgcgttt gcagggctcc ctcgacgtaa accgtcgaac ccttcttcag
      121 atactgctcg gctatcttgg ccaggttttc attgaatatg accacccggt gccattccgt
      181 gcgctccttg cgctcgccag tggccttgtc gcgccaattt tcagaggtgg caaggctcag
      241 gttcacgatc gccttgccgt cctgggtatg gcgcacttca gggtccttcc ccaaattgcc
      301 caccagaata accttgttga ccgaacctgc catgacacat ctccttgttt tcgcgcaccc
      361 tagtctttcg caggcaggct ttcacccgct ggctggattt atccacacag tatatgttct
      421 atttttgttc catacaaggc aaaaacaacc ccagcgcgat ggccggggct gtgaataaat
      481 ctgttaggcg cgaccgaaga ggcgggcaat ccggcccgtg ctcttaattt cgccggcctt
      541 cgcatttgcg agataggcca cagcgtcctt gttctgcatc atggcagtgc tgtaaacgtt
      601 tgaatatgct ccaaaaaccc agttcatcat cagtctcctc aattcaaatc tctgttgttc
      661 tcaatatggg cttttcgccc tcgcagcaca aacggtataa agctacgcaa ccatcagaaa
      721 atgttacagg tcgtgtttac catgtgcgcg cttaagtctc tgcttctgct ccgccgccgg
      781 tttccctggc tgatggtgaa gtgtgcgctg ccccatggcg gtattataaa cgttcgaata
      841 ggcttcaaat atccagttca tgtccatctc ccatgattta gcgggcaatt cgaatatcgg
      901 cccttgtcca gtgggtcaca aacggcaata aacaaccaat cccaacagaa aatgttacag
      961 gtctcccgtg gcgcgccgac tcccctcact caacgctctt aaggctttcg aagccgccgc
     1021 gcggcatgtg agtttcaccg aagcggcggg tgagcttttc gtcactcatg ccgccatcag
     1081 ccgccatatc cgcgaactgg aggagtatct cggcacccag ctcttcctgc gcaccggccg
     1141 cggcgttgcc ctcaccgagg ccggcaagcg ctacggcgag cagctgacgc ccctgtttga
     1201 tgccctgtcc aatgcctcgc gcgaagcggc agcggttggc gccgcccgcc ctctcaatgt
     1261 ctcggttgag ccggccattg cctcgcgctg gctggtgccg cgcctcgggc atttcaagga
     1321 actgcatccc gatattgaac tcaatatcga tcccaccacc cgcttggtgg atttccgcgt
     1381 cgacgaggtt gatctcggca tccgctatgg ccgcggcaat tgggaggacg tggaaatgac
     1441 caagctgtca tccaacgtcg tgatctttcc cgtctgctcc cctgctctca ttgccggtgt
     1501 tgaaaacctg aagccggagg atctcaagaa cttcaatctg ctccatgagc agcgcaagca
     1561 gtggtgggct gaatggctca atgccaacgg catcgaaggc gtcaccgact ggaagggcac
     1621 tgtcttccag aaccatctcg ccattgaggc cgccgagtca gggcaaggct ttgccctcgg
     1681 tgatcaaatt ctatgcactg actccattgt tgaaggctgg ctcgcccgtc cctttgccct
     1741 tgaaatgaaa gaccatggca actattggat agtccgcgcc aagggctcca aggaaaccgc
     1801 aacggcgcgc tccttccgcg aatggctgac aagtgaaatg gccgagacca acaagaaata
     1861 tgccgcattg aaggcttcct ctaagcccgc caaacaggca atagcaccag ccggactgtt
     1921 ctatccccag tcgaatcatt aagcgccggc atctctcaca ccccaaggaa tcctgatggc
     1981 ccgaaaactg tatgactgca tgaaatgccc gggctattgc tgctcctacc ccgtcattga
     2041 agtgaaggac cgcgatgccg aacgcatcgc caggcatttt gctttgccgc tcgccaaggt
     2101 cgagaaaaag ttcttcaaga aggcccacgg ctacaagcgc atcatgcgcc gcaagaagga
     2161 tgagatttat ggcaagatct gccagttctt caacaccacg gaacgccgct gcaccatcta
     2221 tcatgccagg ccctccacct gccgcgcctt ccctggcgac ggccactgcg gctattatga
     2281 tttcctgaaa ttcgagcgcg acgggcagga agatgagaat tacatctcga cgacgaacca
     2341 ttccggaaac tgattttatc ttcttcaatc ccggcgatag agaagtggtg agcccatgac
     2401 gtcacacatc aactatcttg ttctatctga tttgactgcc gcagaccgcg ccaccctgct
     2461 ccggcgcacc gaaagcgatt tgaagccctt catcgaaaag gtgaagccca tcatcgatgc
     2521 cgtgcgcacc gagggtgaca gggccctgtc ccgctttacc gagaaattcg acggcgccct
     2581 cgtcgaagaa agcgccatcg ctgcaactcc cgaagatttc gaccgcgccc accgcgaact
     2641 ggaagcagac gtgagggaag ccatcgtctt tgccgccgcc aacatccgca agttccatga
     2701 actgcaaaaa cccgaagcat cgtggagcgc cgaaacccgc cccggcctcc atgccggtga
     2761 ccgaaccacg cccatcgcct ctgtcgcctg ctacatcccg cgcggcaagg gatctttccc
     2821 gtcctcggtg ctgatgacct gcattcccgc cagtgtcgcc ggcgttgaag acatctgcat
     2881 cgtaacgccg ccgggccccg atggccgcat tgatgcagcc tcgctggttg ccgcccgcgc
     2941 cgccggcgtg agcaaggtct acaaggttgg cggcgcccag gccatcgccg ccgtggctta
     3001 cggcaccaaa accatttcca gatatgtcaa agttgtgggg cctggttcgc cctgggtcgt
     3061 ggccgccaaa cgattgctcg ctgatcagat cgacaccggc acccctgcag gacccagcga
     3121 agcaatcatc tttgccgacg aaacaaccga tggccgcctc gccgccctgg acctgctcat
     3181 cgaggctgag cacggcgacg acagttccgc cttcatcgtc accaccagca gggatgtggc
     3241 cgacgcggcg ctggctgcaa tccccaccta ttggcacagg atgagcgccc agcgcgtgaa
     3301 cttcaccaag gctgtcctga tttcaagtcg cggcggcatt cttctggcga aagatgcagc
     3361 cgaagccatg gccttcatca atgactacgc gcccgagcat ctgcaaatcc tctccaagga
     3421 accacgcaaa tatctgccga tgatcaaaaa tgccggagaa atcctgctcg gcgaacacac
     3481 gccttcgacc ctgggaaatt ttgttctcgg ccccagccat gtcctgccca ccagcggttg
     3541 ggccaagacc ttctccgccc tgtctgtgca tgatttcatg aagcgcacat ccatcgtgga
     3601 ggtcaccgcc caggcctatc ccgaactcgc aagacacgct gccgtcatcg ccagatacga
     3661 aggcttcgat gcccatgcca acgcggtgtc ggacttgcgc aagaagctgc tgggaaactg
     3721 attttcagaa aaatcagccc cgctgcggct atgctgcgcc agcggggccc tcgatccttc
     3781 gagtatgtca cttgatggaa tccggactac accggattac tggcgaggct gggagaataa
     3841 cttgcctcgc atgagcagag ttatgtgaac tgcatgccgg tcaagcgtcg aaatttgctg
     3901 cagcgcgcta accggaatgt gaccgcgaga cctgccgcga tccggggtgg tgaccaggtg
     3961 gcgtcatgct ccctccgatc tgtgtcttaa tgcatcacct gctccgtgga gttcggtgat
     4021 gctttgcatt ttggacccgc cttccacaat gtcaagtaaa ctatccatga aaaacgcttc
     4081 gcaatttcca ccacccgtgt cgtttcagct cactacaggg cgtcatccgc aggctaatca
     4141 tgtgaccatg aacacggcaa cccacagcat tcctctcgcc gatcacgccg gcagcaggcg
     4201 ccgtggcatt cttcttgtcg cgggctccgc cattgtctgg agctttggtg gcgccattgc
     4261 ccgcttcctc gaggtagaca acagttggac aatcgttttc tggcgttctc ttttcgccgc
     4321 cctttttctt ttgggattca tgctctggcg cgatggtcca cgcggcacag ttgcgctgtt
     4381 caaaggcatg gggctggcgg gcctcggcgt tgccctctgc tttgctatcg cctccagttc
     4441 cttcgtggtg gcgcttgcct atacgacagt ggccaacatc ctgctcatgc aggccggcgt
     4501 tcccctgatc gccgcccttc tcacctggac cctgtttcgt gaaagaatct caggcgcaac
     4561 ctgggccgcc attgccgcgg tgattgccgg ggttgccatc atggtctcgg aatccttcac
     4621 tggaaaagtc tctcccatcg gcgacggcct ggcccttctc atctccgtga tgtttgccat
     4681 tgccacggtc atcacccggc gccacgccca cgtacgcatg accccagcag tatgcctcgg
     4741 aaccatcctt gctgcgtgtt tcgcgggact gctcgccggc cactatgcgg tcagtgccgc
     4801 ggatctcggc tggctcgtta cctttggcgc gctcaatctg ggcctgggcc ttgtcatgtt
     4861 cacaaccggc gtcaggctgc tccccgccgc ccttgcagcc cttgtcggaa ccctggaacc
     4921 ggtcctcggc cctatctggg tttggctggt ccacaatgag attccctccg ggcgaactct
     4981 gcttggcggc gccgtggtct tcaccgcgct ggtcattcat ttgctaattg actggcagcg
     5041 gcagtctcgc gcgcaaagcc gctaatc
//
LOCUS       DAORVN010000045         5002 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_11840_length_5002_cov_3.659187, whole genome shotgun sequence.
ACCESSION   DAORVN010000045 DAORVN010000000
VERSION     DAORVN010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 5002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5002
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11840_length_5002_cov_3.659187"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            364..951
                     /locus_tag="PKV23_01485"
     CDS             364..951
                     /locus_tag="PKV23_01485"
                     /inference="COORDINATES: protein motif:HMM:NF014591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HQX84133.1"
                     /translation="MNIILASTSKIRGKILSAAGIRFSVLDSELDEAVAKSSLVKLSP
                     RKMSLQLAKLKAEKVSSQHPEAITIGADQVLGFKNRSFDKPVSRADAESQLSMLRNAT
                     HTLHSAVSCAIAGAEVWNHCSEARLTMRDFTAGFLTSYLDNSPADYLSSVGAYKLEET
                     GIQLFEKIEGDYFAILGLPLLPLLAFLRERKIIAS"
     gene            948..1787
                     /locus_tag="PKV23_01490"
     CDS             948..1787
                     /locus_tag="PKV23_01490"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012110142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HQX84134.1"
                     /translation="MKKACVIGWPIEHSRSPLIHNYWLKKHGINAIYEKKAVEPENVA
                     RFIAGLALSEFVGCNVTIPHKESAFQAVAETDKIARRLGAVNTVYVRNGMACGTNTDG
                     EGFIASLRQLHPDVSLQGTTATVIGAGGAAKAIIGALIDEGVTRIGIINRTRERILGL
                     QEQFGSQIIEINETAAEQALATSDLLINTTSLGMEGGTALALDMRYLKPGALVADIIY
                     APLETALLTKAKERGHPVLGGLGMLLHQAVRGFELWFGVKPEVTKELYDLVAADVQKA
                     KRT"
     gene            1784..2389
                     /gene="coaE"
                     /locus_tag="PKV23_01495"
     CDS             1784..2389
                     /gene="coaE"
                     /locus_tag="PKV23_01495"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013892126.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HQX84135.1"
                     /translation="MITVGLTGSIATGKTEVAKLFAAAGIPVFDSDAEVHALYAGKAA
                     AELLGRTFPGVVVDGKIDRQRLGQAVLGSPEDLRKLESLIHPLVRKKQEDFIARWQKQ
                     RASIVVLDIPLLFETGREKELDYVIVVSADGGIQRKRALARPGMTEEKLASILARQMP
                     DAEKRRRADFVIENSGSLEHLRLQVDALSLKLAEIAGNNRP"
     gene            2386..3072
                     /gene="dnaQ"
                     /locus_tag="PKV23_01500"
     CDS             2386..3072
                     /gene="dnaQ"
                     /locus_tag="PKV23_01500"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009338598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HQX84136.1"
                     /translation="MREIVLDTETTGLDPLQGHRLVEIGAVELFNHIPTGKTYHSFIN
                     PQRDVPREAEAVHGLGDAFLKDKPVFAKIADEFLEFIGDTVLVIHNASFDVGFLNAEL
                     GFIKRPPILYERIIDTLALAKKRHPMGPNSLDALCKRYGIDTAKRTKHGALLDSELLA
                     SVYLELIGGRQITLTLPKTDNVRHLQTGSAPLLAKRSQPLPERLTSEELARHQALVKS
                     LGASALWKAT"
     gene            complement(3074..3658)
                     /gene="secB"
                     /locus_tag="PKV23_01505"
     CDS             complement(3074..3658)
                     /gene="secB"
                     /locus_tag="PKV23_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009450982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051262 - protein tetramerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HQX84137.1"
                     /translation="MAKAAKPAPRKTAAKAANGGKNPDDKTPSISEGKASGAVNLQVP
                     PPQPGAQPSMRILGQYLKDLSFENPNAPQALAPQQSQPDINIAVNVNARNIAPTDFEV
                     ELHLDAKATSDGKVMFASELLFAGVFRMENFPANMLHAAVLIECPRMLFPFARQILAD
                     ATRNGGFPPLMLDPIDFAGMYQKRMQQQKPAAQA"
     gene            complement(3746..4198)
                     /locus_tag="PKV23_01510"
     CDS             complement(3746..4198)
                     /locus_tag="PKV23_01510"
                     /inference="COORDINATES: protein motif:HMM:NF016102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HQX84138.1"
                     /translation="MRRHLPILILALLFADLASLIWLGRFVGVLPVLALVVLGILAGS
                     GLIRRSGANIFTMLNTPAGDKRTVSGGAAKSVLGALAGVLLIIPGIISDFIALALLLP
                     AVRQRVARFFEAHVTGDFTVHRGGKIIDVEAVEIEEMPKLKPVTPRED"
     gene            4319..>5002
                     /locus_tag="PKV23_01515"
     CDS             4319..>5002
                     /locus_tag="PKV23_01515"
                     /inference="COORDINATES: protein motif:HMM:NF033779.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tim44/TimA family putative adaptor protein"
                     /protein_id="HQX84139.1"
                     /translation="MNELFDPLNLVLAAVALMVVWRLRSVLGQRTGTERPPIDPYAPP
                     RRPNGSAPVETGGGKVLEFPSSKEELAKKAEPDAEPKAPIWTGFAKAESPLAAGFEKL
                     AQADPGFSPREFLEGAKLAYEMIVEAFARGNKPALKNLLSKDVYDGFSKAIDARAQAG
                     EKLDFQFVGFEKVDFMSVSLAGKRASIVVKFASQMISATHDKAGALIDGNPKEVRDIT
                     DIWTFERDVA"
BASE COUNT         1150 a         1417 c         1404 g         1031 t
ORIGIN      
        1 tctcaataaa actttctatt aattgaatat gatgttgcag acccgggaac gggatcaaaa
       61 accagtccga ctcttatgcg cgatgatgcg atcctgacca acacttcttt gccaacaaaa
      121 agacaccgca aaaattatct gaacagattc aaattgttag ttagaaaaat ggctgggaaa
      181 ataaagttat ccatcgttat aaacatcgct gacttatccc cacaacagtg ggaatgtgca
      241 ttccgcggat tgccttgttg ataacaatct cacaatatta accgaaagtt aaccatttca
      301 ccatcggcgg aattttccac aatccacctg ttagtactcc acagctttcg acaagagatt
      361 tcattgaata ttattctcgc ttcaaccagc aagattcgcg gaaaaatcct gagcgctgcc
      421 gggataagat tttctgtgct cgattcggag cttgatgaag ccgtcgcaaa aagctccctc
      481 gtgaaacttt caccgcggaa aatgtcgctg cagctggcca agcttaaggc agaaaaagta
      541 tccagccaac accccgaagc gatcacaatc ggcgctgacc aggtgcttgg attcaagaac
      601 cggagtttcg ataagccggt ctcccgcgcc gatgccgaaa gtcagttgtc catgttgcga
      661 aacgcaacac acacattgca ttcagcggtg tcttgtgcca tcgccggtgc cgaagtgtgg
      721 aaccattgct ccgaggcgcg attgaccatg agagacttta ccgcgggctt tttgacgtcc
      781 tatctggata acagcccggc tgattatctg tcttcggtcg gcgcctataa gctggaagaa
      841 accggcattc agctgttcga aaagattgaa ggcgattatt tcgcaatcct tggcctcccg
      901 ctcctgccgc tccttgcatt tttgcgggag cggaagatca ttgcgtcatg aagaaagcat
      961 gcgtcattgg ctggcccatt gagcattcac gctcgccgct catccacaac tattggctga
     1021 aaaagcacgg catcaacgca atctatgaaa aaaaggccgt tgaaccggaa aatgtagcgc
     1081 gcttcatcgc cggactggcg ctgtccgaat tcgtaggctg caacgtgacc attccccaca
     1141 aggaatctgc ctttcaggcc gtggccgaga ccgacaagat cgcaaggcgc cttggcgcgg
     1201 tgaacacggt ctatgtcagg aatggcatgg cctgcggaac caataccgat ggcgaaggct
     1261 tcatcgcatc cctccgccaa ctgcatccag acgtgagcct gcaaggcacc acggcaacgg
     1321 tcataggtgc cggaggtgcg gcaaaggcaa tcatcggcgc gctcatcgat gagggcgtga
     1381 cacggatcgg aatcatcaac cgcacccgcg agcggattct tggcctccag gagcagttcg
     1441 gctcccagat catcgaaatc aatgaaaccg ccgccgaaca ggcattggca accagtgatc
     1501 tcctgatcaa caccacatcc ctgggcatgg aagggggcac ggccctggcc ctggacatgc
     1561 gctacctaaa gcccggcgcg cttgttgccg acatcattta cgcgcctctc gaaacagccc
     1621 ttctaacaaa ggcgaaagaa cgcgggcatc cggtccttgg cggcttggga atgctgctgc
     1681 accaggcggt gcggggattt gaactctggt ttggggtcaa gccggaggtg acgaaagagc
     1741 tctatgatct ggtggccgcc gacgttcaga aagcgaagcg gacatgatca ccgtgggcct
     1801 gaccggctcc atagccactg gcaagaccga ggttgccaaa ctgtttgctg cggcgggaat
     1861 ccccgtgttt gattcagatg ccgaggtgca tgcgctttac gccgggaaag ccgccgccga
     1921 actgctgggc agaacttttc cgggtgtggt cgttgacggc aaaattgacc gccagcgcct
     1981 ggggcaagcg gtcttgggat ctccggaaga tttaaggaag cttgaatcac ttatccatcc
     2041 ccttgtgaga aaaaagcagg aggacttcat cgcccgttgg caaaaacagc gcgcctccat
     2101 cgtggtgctg gatatcccgc ttctcttcga aacaggccgg gagaaggagc tggattatgt
     2161 gattgtcgtg tctgccgatg gcggcatcca gcgaaagcgc gccctggcgc ggccgggaat
     2221 gacggaagaa aagctcgcaa gcattctggc caggcaaatg cccgatgcag aaaagcgtcg
     2281 ccgcgctgat tttgtcattg aaaattccgg tagcctagag cacttgcggt tacaggttga
     2341 tgcgctaagc ctgaagcttg cggaaattgc cgggaataac aggccatgag agaaattgta
     2401 ctcgatacgg aaacaaccgg gcttgatccc ctgcagggcc accgccttgt ggaaatcggc
     2461 gctgttgagt tgttcaatca cattcccacg ggaaaaacct accacagctt tatcaatccc
     2521 cagcgcgacg tgccgcgcga agccgaggcg gttcacggat tgggcgatgc gtttctgaag
     2581 gacaaaccgg tcttcgcgaa gatcgccgat gagtttctcg agttcattgg cgacacggtg
     2641 ctggtcatcc ataacgccag ctttgatgtc ggttttctca atgccgaact gggattcatc
     2701 aagcgcccgc ctattcttta cgagcggatc attgatacgc tggcgctcgc caagaagcgc
     2761 catcccatgg gccctaacag ccttgatgcg ctctgcaagc gttacggcat agatactgcc
     2821 aaacgcacca agcatggggc tctgctggac tcggaactgc ttgcaagcgt ctatcttgag
     2881 ctgatcggcg gccgccagat caccctgacc ttgcccaaaa ccgacaatgt ccgccatctc
     2941 caaaccggca gcgctccgct gctggccaaa cgaagccaac cgcttccgga gcgcctgacc
     3001 agtgaagaac tggcgcgcca tcaggcactg gtcaaaagcc tgggcgcttc agccctctgg
     3061 aaagccacgt gaattaagcc tgcgcggccg gtttttgctg ttgcatgcgc ttttgataca
     3121 tgccggcaaa atcgatgggg tccagcatca gcggcggaaa tccgccattg cgcgtggcgt
     3181 ccgcaagaat ctgccgggcg aacgggaaca gcatccgcgg gcactcgatg aggacagccg
     3241 catgaagcat gttggccggg aaattctcca tgcggaaaac cccggcgaat agcagctccg
     3301 aggcgaacat gacttttccg tcggaggtgg cctttgcatc gagatggagc tcaacctcga
     3361 aatccgtggg tgcgatgttg cgggcgttca cattgaccgc gatattgatg tcgggttggc
     3421 tttgctgcgg cgccagggcc tggggcgcat tgggattttc aaagctcagg tcttttaaat
     3481 actggccaag gatgcgcatt gaaggctggg cccccggctg gggcggcgga acctgcagat
     3541 tgaccgctcc gcttgccttg ccttcggaga tgctcggcgt cttgtcgtcc ggattcttgc
     3601 cgccgtttgc tgccttcgcc gcggttttgc ggggagccgg ttttgctgct ttagccatct
     3661 ggaatgcctc tcatgcgccg aaatagtcgg gtcaaatcac gttgcagtgg ctaacatgaa
     3721 ggcagcaggg ccgcaagcag ggtctttaat cttcccgtgg agtgaccggc ttcagctttg
     3781 gcatttcctc gatttccacc gcttccacgt cgatgatttt tccgccccgg tggaccgtga
     3841 agtccccggt cacatgcgcc tcgaaaaacc gcgccacgcg ctggcgcacc gccggcagca
     3901 gcagggccaa ggcaatgaaa tcgctgataa tccccggaat aatcagcagg accccggcga
     3961 gcgctccaag cacgcttttg gctgccccgc ctgataccgt ccttttgtcg ccggctggtg
     4021 tattgagcat ggtgaaaata tttgcgccgc tccgccggat aagtccgctg cccgcaagaa
     4081 tgcccagcac gaccagtgcc aggacaggca ggacgcccac aaagcgcccg agccagatga
     4141 gggacgccag gtcggcgaac agcagggcga ggatgaggat tggcaagtgg cggcgcaagc
     4201 aagactccaa tagaatggtc cttggaccta tcccaaagtc tcctatatag gactcaaccc
     4261 aagacgagta caatgtgcgc cgctccgtgt gtaggctcag aagtgtgaga aattggtaat
     4321 gaacgagctc tttgatcccc tgaatctggt actcgccgcc gtggcgctca tggttgtgtg
     4381 gcgcctgcgt tcggttctcg gccagcgcac cgggaccgag cgaccgccga ttgatcccta
     4441 tgccccgccc cgccgcccca atggcagcgc cccggttgaa accggcggcg gaaaagttct
     4501 ggaatttccc tccagcaagg aagaactggc gaaaaaagcc gaacccgacg cggagccaaa
     4561 ggccccgatc tggaccggct ttgccaaggc ggaatcgccg ctggccgccg gatttgaaaa
     4621 actggcgcag gccgatccgg gattttcacc aagggaattc ctcgaaggcg ccaagcttgc
     4681 ctatgagatg attgtcgagg ccttcgccag gggcaataag ccggccctga agaacctcct
     4741 gtcgaaggac gtttatgacg gcttttcgaa agccatagat gcgcgcgccc aggccggcga
     4801 aaagctcgat ttccaattcg tcggttttga aaaggtcgac ttcatgtccg tgtcgcttgc
     4861 cggaaagcgc gccagcatcg tcgtcaagtt cgccagccag atgatttccg caacccatga
     4921 caaggcgggc gccctcatcg acggcaatcc caaggaagtg cgtgacatca ccgatatctg
     4981 gacctttgaa agggacgtgg cg
//
LOCUS       DAORVN010000046         4981 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_11930_length_4981_cov_2.947828, whole genome shotgun sequence.
ACCESSION   DAORVN010000046 DAORVN010000000
VERSION     DAORVN010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 4981)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4981)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4981
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11930_length_4981_cov_2.947828"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            121..765
                     /locus_tag="PKV23_01520"
     CDS             121..765
                     /locus_tag="PKV23_01520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX84140.1"
                     /translation="MRNFVQIVFVLSALGLWTGLAEAETKTFENPMIGTNRLDWCVDW
                     GKGCGQEAATAWCKSNAYDHSVAGKQAPDIGASSPTKLIATGEICDQSYCDGFTSITC
                     VTADLPAVAQPAAPETPAPDAEAPAPATVPVTAESKSELFKNPMHEQVRLNWCFSGDG
                     GCGKKAAEAWCAQQGFSSAKDFKYVSGIKPTIQIGTGKTNKKASKAFSRVTCLN"
     gene            complement(766..1350)
                     /locus_tag="PKV23_01525"
     CDS             complement(766..1350)
                     /locus_tag="PKV23_01525"
                     /inference="COORDINATES: protein motif:HMM:NF002099.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2585 domain-containing protein"
                     /protein_id="HQX84141.1"
                     /translation="MSSDSRKAFWLALWGPVLAAGIVLAIAIILLAMGREPICKCGYV
                     KLWHGVVMSSENSQHVSDWYTPSHIIHVFFFYGLFWLVGKKWPFALRLCLALVIEGAW
                     EIFENTDFIINRYREATISLDYYGDSVINSVSDVIAMAIGFYAASRLPVWVTIILALA
                     MELLVGMAIRDNLALNIIMLIYPLDAIKAWQGGG"
     gene            complement(1394..1819)
                     /locus_tag="PKV23_01530"
     CDS             complement(1394..1819)
                     /locus_tag="PKV23_01530"
                     /inference="COORDINATES: protein motif:HMM:NF027482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4864 domain-containing protein"
                     /protein_id="HQX84142.1"
                     /translation="MLRSVLMIVLLGFLSVTAFTQSISETEKTEFQRIITAQISAFRA
                     DDGQTAYGYAAPVVRNIFPTPEIFMSMVKRGYGPVYRPQSFSFTEALIDATGRPAQKM
                     LVVGPDGKTYEALYSMEKQPDGTWRISGCTLLEVPGLDA"
     gene            1909..2229
                     /locus_tag="PKV23_01535"
     CDS             1909..2229
                     /locus_tag="PKV23_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HQX84143.1"
                     /translation="MASNDVLEQASTWLAEGRKVALATVIETWGSAPQPVGSQLAIDG
                     DGNFAGSVSGGCVEGAVVSEALDAIASGQPKVLAFGVADETAWQVGLACGGKIRVFVE
                     PLGS"
     gene            2226..2894
                     /locus_tag="PKV23_01540"
     CDS             2226..2894
                     /locus_tag="PKV23_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HQX84144.1"
                     /translation="MKRELLKAVIEAKRQRRAVAVVTTIENGEQRLVARENIGKDPLG
                     AVLEEAFRFDQSGLHGGEFVHIHNPPLRLVVIGAVHIAQGMIPMARQAGYDITVIDPR
                     GAFATGARFPDITLHTTWPEDVLPGLGVDARTALLALTHDPKIDDPALSIALKSECFY
                     IGALGSKKTQASRVERLQKLGFSDAEIARIHGPIGLPIGAKGAAEIAISIMAEMTRSL
                     RLGN"
     gene            2896..4512
                     /locus_tag="PKV23_01545"
     CDS             2896..4512
                     /locus_tag="PKV23_01545"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding/glycosyltransferase family
                     2 protein"
                     /protein_id="HQX84145.1"
                     /translation="MKFGELPLQDTQGCILAHSVRHAEGVFKKGRKLAANDLKLLAAS
                     NVSMVFVAKLDANDVPEDEAARTLAGKIAGSGASAQEPFTGRANVYAQQRGLVVVDGL
                     RVNAINRVHESMTLATLPSYHVVVKGQMIATVKIIPFAVGKENLNKVLTEIGTKPVIR
                     VQALAERRVGLVITKVAGSRLSLIEKSETAMRERVTALGSGLAEVRVCDHSIEAVRTS
                     VKELEALSCNPILLFGASAIVDREDVIPAGLSAAGGKVVHLGMPVDPGNLMMLGDLRG
                     VPVLGVPSCARSPKVNGFDWALERVLAGIPLSSGDIMDMGAGGLLAEISSRPSPRDRK
                     PVAQHAPRIAAIVLAAGKSSRMGSNKLLAELHGKPLLRHSVEALKASSVNDIIVVTGN
                     EPERVQSALKPLDVTLVHNANFAEGLSTSLKRGLAAVPAETDAVLICLGDMPLVDAQT
                     IDRLVAAFNVPEHRTICVPTFEGKRGNPVLWGRQHFIAIDEIEGDQGARLLLDALSDE
                     VVEVEVKTQAVLVDVDTPQALEEIRSALNP"
     gene            complement(4490..>4981)
                     /gene="radC"
                     /locus_tag="PKV23_01550"
     CDS             complement(4490..>4981)
                     /gene="radC"
                     /locus_tag="PKV23_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012336381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HQX84146.1"
                     /translation="SAAPERLKEVDGVGEAITTELKLIRAAALRLMKGGIVNRPLLSS
                     WSAILAYCRASMAFHDKEQFRILFLDKKNQLIADEVQQEGTVDHTPVYTREVMKRALE
                     LSASAIILAHNHPSGDPTPSVADIDMTRKIIDAGLKLGITVHDHVIVGREGHTSFRGS
                     KLI"
BASE COUNT         1046 a         1304 c         1601 g         1030 t
ORIGIN      
        1 attatctgat cgagcctgtt gattcgaaaa agtcagatgg gcgttccttt ggacattaaa
       61 agcttgcgtc ctgatggtgg tttgaggcag agggttcttc ccagggattg gagagccgtg
      121 atgcggaatt ttgtgcagat tgtttttgtg ctgtcggcgc ttggcctttg gactgggctt
      181 gcagaagctg aaaccaagac ttttgaaaac cccatgattg gcaccaatcg cctggattgg
      241 tgtgtggatt ggggcaaggg ctgcgggcaa gaggcggcaa cggcatggtg caagagcaat
      301 gcctatgatc attccgtggc cgggaagcag gcgccggata tcggcgcttc gtcaccgacg
      361 aagttgattg ccacgggaga aatctgcgat cagagctatt gtgacggctt cacctccatt
      421 acctgcgtca cggccgacct gcctgcagtg gcgcagccag cggcgccgga gacgcctgcg
      481 ccagatgccg aagcgccggc tcctgccacg gtgcccgtca ctgcggagag caagagcgaa
      541 ttgttcaaga atccgatgca tgaacaggtc cggctgaact ggtgtttcag cggggacgga
      601 gggtgcggga aaaaagccgc cgaggcgtgg tgcgcccaac aggggttcag ctccgccaag
      661 gatttcaagt atgtcagcgg catcaagccc accatccaga ttggcactgg aaaaaccaat
      721 aaaaaggcca gcaaggcttt cagccgtgtg acctgcctga attagtcagc cgccgccctg
      781 ccacgccttg atcgcatcga ggggatagat gagcatgatg atgttgagcg caaggttatc
      841 gcggatagcc atgccaacca gcaattccat ggccagtgcc aggattatgg tcacccatac
      901 ggggaggcgg gacgccgcgt agaacccaat cgccatggca atgacatcgc tgactgaatt
      961 tatgacgctg tcgccatagt aatcgagcga gatcgtggct tcgcggtaac ggttgatgat
     1021 gaaatccgta ttctcgaaga tttcccatgc gccttcgatc acgagagcaa gacagaggcg
     1081 caatgcgaat ggccattttt tgccgaccag ccagaacagg ccgtaaaaga aaaagacatg
     1141 gatgatgtgc gatggggtgt accagtcact gacgtgctgg gagttttccg aactcatcac
     1201 cacaccgtgc cagagcttga cgtagccgca cttgcagatg ggttcgcggc ccatggccag
     1261 caggatgatg gcgatggcca gaacaatccc ggcagccagg acaggccccc aaagggcaag
     1321 ccaaaacgct ttccgtgaat cagatgacat ggcctgacaa tggcacgcct tccgtgcggt
     1381 ggctaacagg agatcaggcg tcgaggccgg ggacttcgag cagggtgcag ccggaaatgc
     1441 gccaggtgcc gtccggctgt ttttccatgg agtaaagtgc ctcgtaggtc ttgccgtcgg
     1501 ggccgacaac gagcattttt tgggccggcc tgcccgtggc gtcgatcagg gcttcagtaa
     1561 aactgaagga ctgcgggcga tagaccggac cgtaaccccg cttcaccatg gacatgaaga
     1621 tttccggggt ggggaagatg ttgcggacaa cgggcgctgc atagccgtaa gcggtttgcc
     1681 catcatcggc ccggaaggca ctgatttggg ctgtaattat gcgttgaaat tcggtctttt
     1741 cagtctcact tatggactgc gtgaaggccg tcacggacag gaagcccaaa agcacgatca
     1801 tgaggacaga gcgaagcatc atgggcctcc cgcaatatga gcatcttgac acatatacga
     1861 gggcatgtcc tgtgtggttt cacctgaggt ttgcgacagg aggacaaatt ggccagcaac
     1921 gacgttcttg aacaggcctc cacttggctg gcggaggggc ggaaagttgc gcttgcaacg
     1981 gtcatcgaaa cctggggttc ggcgccgcag ccggtaggca gccagctggc cattgacggc
     2041 gatggcaatt ttgccggttc cgtatcgggc ggctgtgttg aaggcgcggt ggtgagcgaa
     2101 gcgcttgatg ccattgcctc gggccagccg aaagttctgg cctttggtgt ggctgacgaa
     2161 acggcctggc aggtcggcct agcctgcggc ggcaagatac gggtatttgt ggagccgctg
     2221 gggtcgtgaa acgggagctt ctgaaagccg tgatcgaggc aaagcggcag cgccgagcgg
     2281 ttgccgtggt cacaaccatt gagaatggcg agcagcggct ggtggccagg gaaaatattg
     2341 ggaaggatcc gcttggcgcc gttctcgaag aagcttttcg ctttgaccag tcgggacttc
     2401 atggcggaga atttgtacac atccataacc cgccgttgcg gcttgttgtt atcggcgcag
     2461 tccacatcgc gcagggcatg atcccaatgg cccgccaggc cggctatgac attacggtta
     2521 ttgatccgcg tggggccttt gcgacaggcg cgcggtttcc cgacataacg ctgcacacca
     2581 cctggccgga ggacgttttg ccgggcctcg gagtcgatgc gcgcacggcg cttctggcgc
     2641 tgacccatga tccaaagatt gatgatccgg ctctttccat cgcgctgaaa tccgagtgct
     2701 tttatatagg tgccctgggt tccaagaaaa cccaggcgag ccgggttgaa cgcttgcaga
     2761 agttgggatt ttccgatgcg gagattgccc ggatccacgg gcctatcggc ttgcccatcg
     2821 gagccaaggg cgctgccgaa atcgccattt ccatcatggc tgaaatgacc cgttccttgc
     2881 gcctgggcaa ctgacatgaa atttggcgaa ctgccgctgc aagacacaca gggatgcatc
     2941 ctggcgcatt cggtgcggca tgcggaaggc gtgttcaaga aagggcggaa gcttgcggca
     3001 aatgatttga aactcctcgc agcgtccaat gtttcaatgg tgttcgtggc caagctcgat
     3061 gccaatgacg ttcccgagga tgaggcggcg cgaacgctag caggaaagat tgccggttcg
     3121 ggagcttcgg cccaggaacc cttcacgggg cgggccaatg tttatgccca gcagcgcggg
     3181 cttgtggttg tcgacggttt gcgcgtcaac gcgataaacc gggtgcatga aagcatgacg
     3241 ctggcgacac ttcccagcta ccatgtggtt gtcaaggggc agatgattgc gacggtcaag
     3301 atcattccgt ttgccgtggg aaaggaaaat ctcaacaagg tgcttactga gatcggcact
     3361 aaaccggtga tcagggttca ggctttggcc gagaggcgcg tgggtctggt gatcaccaaa
     3421 gttgccggca gccggctgtc acttatcgaa aaatccgaaa cggcgatgcg cgaacgtgtg
     3481 acggcactgg gcagcggcct ggcggaggtc agggtttgcg atcattccat tgaagcggtg
     3541 cggacatcgg tgaaagaact ggaggcactg tcctgcaatc ccatcctgct gttcggcgca
     3601 tcggcgattg tcgaccggga agatgtgatt ccggcgggac tttcggcggc aggcgggaag
     3661 gtagttcacc tcggcatgcc ggttgatccc ggcaatctga tgatgctggg tgatttgcgt
     3721 ggcgtgccgg tgctgggtgt tccgagttgc gccagatcgc ccaaagtaaa cggctttgat
     3781 tgggcgctgg aacgcgtgct ggccgggatt ccactttcgt ccggtgatat catggatatg
     3841 ggggccggcg gtctgctggc ggagatttcg agccggccat cgccgcgtga ccggaagccc
     3901 gttgcgcagc atgcgccacg gattgcggcc attgttcttg cggcggggaa atcctcgcgc
     3961 atgggcagca acaagcttct ggcggagctt cacggcaaac ccctgctgcg ccacagcgtg
     4021 gaggcgctga aagcctcaag cgttaatgac atcatcgtgg tgacggggaa cgagccagaa
     4081 cgggttcaat ccgccctcaa gccgcttgat gtcaccctgg ttcacaatgc gaatttcgcc
     4141 gaagggcttt cgacgtcctt gaaacgcgga cttgccgccg tgccggcgga gacggatgct
     4201 gtgttgattt gcctcggtga catgccgctg gtggatgcac agacaattga ccggctggtg
     4261 gcggcgttca atgttcccga gcaccgcacc atttgcgttc ccaccttcga aggcaagcgc
     4321 ggcaatccgg tgttgtgggg gcggcagcat ttcatcgcaa ttgacgagat tgaaggtgac
     4381 cagggcgcgc ggctcttgct ggatgcgcta tctgacgagg tggtggaggt tgaagtcaag
     4441 acacaggccg tgctggttga tgtggacacg ccgcaggccc ttgaggagat cagatcagct
     4501 ttgaacccct gaagctggtg tggccctcac gccccacaat cacatggtca tgcacggtga
     4561 tgccgagctt cagtccggca tcaatgattt tccgggtcat gtcgatgtcg gcgacagaag
     4621 gagtgggatc accagacgga tggttatggg ccagaatgat ggcggaagcg gaaagttcaa
     4681 gtgcccgctt catcacctcg cgggtgtaga ccggcgtgtg atcaaccgtg ccttcctgct
     4741 gcacctcgtc ggcgatgagc tggtttttct tgtccagaaa caaaatgcga aactgttcct
     4801 tgtcatggaa agccatggag gcgcggcagt atgccagaat cgcgctccat gacgacagca
     4861 gcgggcgatt aacaatcccg cccttcatca ggcgcaatgc tgcggcacgg atgagcttca
     4921 gctcggtggt gatggcttcg ccaacgccat cgacttcctt caggcgctcg ggagcggcgc
     4981 t
//
LOCUS       DAORVN010000047         4951 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_12044_length_4951_cov_3.172386, whole genome shotgun sequence.
ACCESSION   DAORVN010000047 DAORVN010000000
VERSION     DAORVN010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 4951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4951
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12044_length_4951_cov_3.172386"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..892
                     /locus_tag="PKV23_01555"
     CDS             <1..892
                     /locus_tag="PKV23_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013168459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQX84147.1"
                     /translation="GNSPRIAHAHLPTALIFQNLALFPLMTVSENIAYGLRVRGVDKA
                     ARMKRADELLELIALPDQGKKLVSELSGGQKQRVAIARALAVEPKVLLLDEPLSALDL
                     KLRQHMRNELREIQKRVGITFIYITHDQGEALTMSDRVAVMSDGEILQIADGKTIYDS
                     PATSFVASFVGENNPLPGRVTKAGKTGAVLKTPFGELRGANPHGLKVGDNAILFVRPE
                     SISLGKGAADATITTQVLNVAFEGNVSHIFMKGAGKKDLSVTVGRHGGAEIPARGAKA
                     AVNYDPGYAIVLPEGKMARE"
     gene            885..1886
                     /locus_tag="PKV23_01560"
     CDS             885..1886
                     /locus_tag="PKV23_01560"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013168460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQX84148.1"
                     /translation="MSRFVVFLLPLAVIAAFWLMSRQEAKLAHDPTHKSFFQRNGTVL
                     SIVFIALVTFWMLFLVVMPYLYMVQESFHPRLPPMKRGGPEDFLTIQQYKSFFVEPSS
                     GNWNTLHMRAFFFSILTSVFITAVNFAICYPLAYYMAQAGTIQKVRLLMLCLIVPYWV
                     NEILRAFSLRLLLASKGLINQTLMGIGIIDTPIDFLGSNSGLYIGLSYAYLLIMVFPL
                     YNAIESLDKNQIEAARDLGAPWWKIHRDVVIPYAKPGIASGCTMVFMLSAGSLAAPQF
                     LGGPSTLWFTSIVYDFFFQAFNWPRGAAYAFILLTACIIFVLAILKLFKVSLGETIR"
     gene            1883..2773
                     /locus_tag="PKV23_01565"
     CDS             1883..2773
                     /locus_tag="PKV23_01565"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018388243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQX84149.1"
                     /translation="MNSRLIMRGMIGFYIFLFFAYLFGPLIVMSITAFNTPGYPQAYP
                     FEAFTLDWFVKLWNHRSMMDGIKNSIIIGLGVVAISVPVGLAAAIMMGQIYHRARSIF
                     YLVTVSPVLTPGVIIGISTVIFWKDVMGVTDFTKAYFYKGIILAIFGQASFVAAYCLL
                     IIMARLQRFDRSQEEAALDLGASFPQVFWHILLPFLKPALISAAVIAFLSSFENYNTT
                     TFAILADKTLVTVLAGEVRQGTTPALSALAVVIIGLSLIGAIFYEVAKRREEAAAQRM
                     QERARLAERSEVIGANAELA"
     gene            2770..3870
                     /locus_tag="PKV23_01570"
     CDS             2770..3870
                     /locus_tag="PKV23_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019171041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HQX84150.1"
                     /translation="MKIKSLETFCNQYIGFVRVTTDDGSQGWGQVSTYNADITCDIFH
                     RQVAKWSLGAEALDIDALVALIPEREHKFPGSYLMRAITGLDTALWDLRGKLEKKSVC
                     ELLGGKLRPFPVYASSMKRGEITPEAEAERLSRLRDAHGYTAFKFRVGKECGHDEDEW
                     PGRTDAIVPMVRKALGDSATLLVDANSSYTPKKAIEIGKFLEQHGVVHFEEPCPYWEY
                     DWTKQVTDALSLDVTGGEQDCDLALWKFAMEMRCVDVAQPDICYLGGLSRTLKVVEMA
                     NKAGLPVTPHAANMSMVTVFTLHMMGAISNAGPYVEFSIEGNDYYPWQEDFYRPALVA
                     RDGKVQIPEGPGWGVDLNPQWLDKAKYQKSEL"
     gene            complement(3867..4667)
                     /locus_tag="PKV23_01575"
     CDS             complement(3867..4667)
                     /locus_tag="PKV23_01575"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HQX84151.1"
                     /translation="MKLIHITDLHLVPPGHRLWGLDPFSRLDQCFADIMKYHGDAKFC
                     AISGDLTERGEAESYRALQERLEKLQLPVHLMLGNHDDRANFLRVFNGPLEGGFVQHR
                     LILDGQHFLFLDTLKGGPSSAGLYDGPRRSWLRDALATANGAPVYIFMHHPPFKIHHP
                     LMDLIPLEDGDAFGDLLAGHNVRHIFFGHAHRTISGQWRGISYSALPGLNHQLPLVDG
                     SVPTIYSDEPAMYSVVYLNGDQITVHSDAFMNRAPAQMARDAERGGWS"
     gene            complement(4667..>4951)
                     /locus_tag="PKV23_01580"
     CDS             complement(4667..>4951)
                     /locus_tag="PKV23_01580"
                     /inference="COORDINATES: protein motif:HMM:NF014010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoU domain-containing protein"
                     /protein_id="HQX84152.1"
                     /translation="RLATGVLSSADLEGAKRLIAQKDAFRKLENAVLDQHFKSDKQTK
                     RGALRRSALYVDMIRDLHRINSHIVSAGYPIVEAAGLLRGSRLRNEAKKT"
BASE COUNT          986 a         1472 c         1399 g         1094 t
ORIGIN      
        1 cgggaactct ccgcggatcg cgcacgctca cctccccacc gcgttgatat tccagaacct
       61 ggcgcttttc ccgctcatga ccgtatcgga aaacatcgcc tatggcctgc gcgtgcgcgg
      121 cgtcgacaag gcggcgcgga tgaagcgcgc cgacgaacta cttgagctta ttgccctgcc
      181 ggaccagggc aagaaactgg tcagtgaatt gtccggcggc cagaaacagc gcgtggccat
      241 agcccgggcc cttgcagttg aacccaaggt cctgctgctc gatgagccgc tttccgccct
      301 tgacctgaaa ctgcgccagc atatgcgcaa tgagttgcgg gaaatccaga agcgcgtcgg
      361 catcaccttc atctacatca cccatgacca gggcgaggct ctgaccatgt cggaccgcgt
      421 ggccgtgatg agcgatggtg aaattctgca gatcgccgac ggcaagacca tctatgattc
      481 gcccgccaca tctttcgtgg cctcctttgt tggtgaaaac aatcccttgc ctgggcgtgt
      541 gaccaaggct ggcaagaccg gcgccgtgct gaagacgccc ttcggggaat tgcggggcgc
      601 caacccgcat ggattgaaag tgggcgacaa cgccatcctt tttgtccgtc ccgaatccat
      661 ttctcttggc aagggcgccg ccgatgccac gatcaccacg caggtcctca atgtggcgtt
      721 cgaaggcaat gtcagccaca ttttcatgaa aggcgccggc aagaaggacc tttcagtgac
      781 cgtaggccgc cacggcgggg cggaaatccc ggcaagaggt gccaaggcgg ccgtgaatta
      841 cgatccgggc tatgccatag ttctgccgga agggaagatg gcccgtgagt aggttcgtcg
      901 tctttctgct tcccctggcg gtgatcgccg cattctggct gatgtcacgc caggaagcaa
      961 agcttgccca tgatccaacc cacaagagct tcttccagcg caatggcacg gtgcttagca
     1021 tcgtgtttat agccctggtg accttctgga tgctgtttct ggtggtaatg ccttatcttt
     1081 acatggtgca ggaaagtttc caccccaggc tgccgcccat gaaacgtggt gggcccgagg
     1141 atttcctcac gatccagcag tacaagtctt tcttcgtgga gccgtcgagt ggcaactgga
     1201 acacgctcca catgcgggcg ttctttttct ccattctgac gtcggtgttt atcacggctg
     1261 tcaatttcgc catttgctac ccgctcgcct attacatggc ccaggcaggc accatccaga
     1321 aggtgcgcct gctgatgctc tgcctcattg tgccctattg ggtgaatgaa atcctgcgcg
     1381 ccttttcgtt gcggctgctg ctggcatcca agggcttgat caaccagacg ctcatgggca
     1441 tcggcatcat tgacacgccg attgatttcc tcggcagcaa ctccggcctc tatatcggcc
     1501 tgtcctatgc ctatctgctc atcatggttt ttccgctcta taacgccatc gaaagcctgg
     1561 acaagaacca gattgaagct gcacgcgatc ttggcgcccc atggtggaaa atccaccggg
     1621 acgttgtcat cccctatgcc aagccgggca tcgcctccgg ctgcaccatg gtgttcatgc
     1681 tctcggccgg ttctttggca gcgccccagt tcctgggcgg gccaagcacc ctgtggttca
     1741 cctccattgt ctatgacttc ttcttccagg ctttcaattg gccgcgcggt gctgcttacg
     1801 cattcatcct gctgaccgcc tgcattatct tcgtgttggc cattctcaag ctgttcaaag
     1861 tcagccttgg ggagaccatc cgatgaattc cagacttatc atgcggggga tgattggatt
     1921 ttatattttc ctcttcttcg cctatctgtt cggcccgctg atcgtgatga gcatcacggc
     1981 attcaacacg ccgggttatc cccaagccta tccgtttgag gccttcacgc tcgattggtt
     2041 cgtgaagctc tggaaccacc gctcgatgat ggatggcatc aagaattcga ttatcattgg
     2101 cctgggcgta gtcgccattt cggttcccgt agggttggcg gcggctatca tgatgggcca
     2161 aatatatcac cgcgcccgca gcatattcta tctcgtgacg gtttctcccg tgctcacccc
     2221 cggcgtcatc atcggtattt ccacggtgat tttctggaaa gacgtgatgg gcgttaccga
     2281 tttcaccaag gcctatttct acaagggcat catcctggcg attttcggcc aggccagctt
     2341 cgttgccgcc tattgcctgt tgattatcat ggcccgcctg cagcgcttcg atcgctccca
     2401 ggaagaggca gcccttgacc tcggcgccag cttcccgcag gtcttctggc atatcctgct
     2461 gccgttcctg aagcccgcct tgatttctgc cgccgtgatt gccttcctgt cctcgttcga
     2521 aaactacaac accacgacct ttgccattct ggccgacaag accctggtca cggtcctggc
     2581 cggcgaagtg cgccagggaa ccacgccggc gctttcggcg ctggctgtag tcatcatcgg
     2641 actgtcactt atcggtgcca tcttctatga ggtcgccaag cgccgggaag aagcggccgc
     2701 ccagcgtatg caggaacggg cccgtctggc cgagcgcagc gaggtcatcg gtgcgaatgc
     2761 ggagctggcg tgaaaatcaa gagtcttgag accttctgca accagtatat tggctttgtg
     2821 cgggtcacca ctgacgacgg aagccagggc tggggccagg tttcgaccta taacgctgac
     2881 atcacctgcg acatattcca ccggcaggtt gcaaagtgga gcctcggcgc tgaggcgctc
     2941 gacatcgatg cgcttgtcgc gctgatcccc gagcgcgagc ataaattccc cggttcctat
     3001 ctcatgcgcg ccatcactgg gcttgatacc gccctttggg acctgcgcgg taaacttgaa
     3061 aagaaaagcg tgtgcgagtt gctgggcggc aagctgcggc cctttccggt atatgcctca
     3121 agcatgaagc gcggcgaaat cacccccgaa gcggaagccg agcgcctctc gcgcctgcgc
     3181 gacgcgcacg gttacactgc tttcaaattc cgcgtcggca aggaatgcgg ccatgacgag
     3241 gacgagtggc ccgggcgcac cgacgccata gtccccatgg tgcgcaaagc ccttggtgac
     3301 agcgccacct tgctggtgga cgccaacagc agctacacgc cgaagaaggc tatcgagatc
     3361 ggcaagtttt tggaacagca cggcgtggtt cattttgagg aaccgtgccc ctactgggaa
     3421 tatgactgga ccaagcaggt gaccgatgcg ctgtcgctgg atgtgaccgg cggtgaacag
     3481 gattgcgacc tcgccctgtg gaaatttgcc atggagatgc gctgtgttga cgttgcccag
     3541 cccgacatct gctatctcgg cggcctttca cggaccttga aggtggtgga aatggctaat
     3601 aaggcgggac ttcccgtaac accccatgcc gccaacatgt cgatggtcac cgtattcacc
     3661 ctgcacatga tgggagccat ttccaacgcg ggcccctatg tcgagttctc cattgaaggc
     3721 aacgactact atccctggca ggaagacttc taccggccgg cgcttgtcgc ccgcgatggc
     3781 aaggttcaaa ttccggaagg tccgggctgg ggcgtggacc tcaatccgca atggctggac
     3841 aaggccaaat accagaagtc ggaactttag ctccagccgc cgcgttcggc atcacgcgcc
     3901 atctgcgccg gcgcacggtt cataaaggca tcggaatgaa ccgtaatctg atcaccattc
     3961 agatacacaa ccgaatacat tgccggttca tcgctataaa tcgtgggaac cgagccatca
     4021 accaggggca attggtggtt gaggccgggc agggctgaat aggaaatgcc gcgccactgc
     4081 ccgctgatgg tccggtgcgc atggccgaag aagatgtggc gcacattgtg gcccgctagg
     4141 agatcgccaa acgcatcgcc atcttcaagc gggatcaaat ccatcaaggg atggtgaatc
     4201 ttgaacggtg ggtggtgcat gaaaatgtag accggcgcgc cgtttgctgt ggccaatgcg
     4261 tcccttagcc atgaacgccg cggtccatca tagagaccag cggaagaagg gccgcctttc
     4321 agggtgtcaa ggaacaagaa atgctgtccg tccaggatta gtctgtgttg cacgaagccg
     4381 ccttcaaggg ggccattgaa gaccctcagg aagtttgcgc ggtcatcgtg attgccaagc
     4441 atcagatgaa ccggaagctg gagtttctcc aggcgctcct gcagggcccg gtagctttcg
     4501 gcttcgcccc gctcggtgag atctcccgat atggcacaaa atttcgcatc tccatgatac
     4561 ttcatgatat cggcgaagca ctggtcgagg cgcgagaagg ggtcaagacc ccagagccta
     4621 tggccggggg gcacgagatg aaggtcagtg atatggatga gtttcatcag gtcttcttcg
     4681 cttcatttct caaacgcgag ccgcgcagca atcccgccgc ctcgacaatc gggtagcccg
     4741 ccgaaacaat gtgcgaattg atccggtgca ggtcgcggat catgtcgacg taaagcgcgc
     4801 tgcgccgcag tgccccgcgt ttggtctgct tgtcgctctt gaaatgctgg tccagcaccg
     4861 cattttcaag cttgcggaag gcatcctttt gcgcgatcag ccgtttggcc ccctcgagat
     4921 cggcggaact cagcaccccc gtggcgagcc t
//
LOCUS       DAORVN010000048         4927 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_12137_length_4927_cov_3.221264, whole genome shotgun sequence.
ACCESSION   DAORVN010000048 DAORVN010000000
VERSION     DAORVN010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 4927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4927
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12137_length_4927_cov_3.221264"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..338)
                     /locus_tag="PKV23_01585"
     CDS             complement(<1..338)
                     /locus_tag="PKV23_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011582062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX84153.1"
                     /translation="MSQSLHMLPEGMREPHGLYHPRNEHDACGIGLIANINNIKSHRT
                     IDDGLSILKNLEHRGAVGADPKAGDGAGIMIQIPHKFFSEEAKRLGFELPAPHTYGVG
                     YLFMPRETVYR"
     gene            789..1844
                     /locus_tag="PKV23_01590"
     CDS             789..1844
                     /locus_tag="PKV23_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low specificity L-threonine aldolase"
                     /protein_id="HQX84154.1"
                     /translation="MNFASDNHYGVHPRILEAMVEANAGSAPAYGGDDWTKKAESQLS
                     KIFEKEVRSFLVTTGTAANGLALSALTPPYGAVICHGEAHISVDECNSPEMFTGGAKI
                     LGLHGPAGKITPAMVEKTLKGFIRGEHDPKPAAVSLTNSTELGTTYSPDDVKAISNLI
                     KPRGMKLHMDGARFANAVAGLGVTPAELTWKSGVDALSFGATKNGAMMLEAVVFFDTN
                     LADDFIYRRMRGGQLVSKGRYLGGQMLAYLKDGLWLENARQANGLAAQLAEGLGKFKN
                     IRIPNPVQANEVFAVMPRALNDKLLASGAKFYDWMPDSLGKAIADDEIFVRFVLSYAT
                     PEADVRKFLDLAKDLQS"
     gene            complement(1892..2344)
                     /locus_tag="PKV23_01595"
     CDS             complement(1892..2344)
                     /locus_tag="PKV23_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015928411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20 family protein"
                     /protein_id="HQX84155.1"
                     /translation="MFDFTPLYRSSVGFDHLARMLDDAASFETPAYPPYNIERVGEDE
                     YRITIAIAGFGASDINLEVKGNALTVTGKKAEKPAEKTEYLHQGIAARNFERRFQLAD
                     HVEVTGADLNHGLLHISLKREIPEALKPRTIAIRTANEQKVLEAKKAA"
     gene            2572..2922
                     /locus_tag="PKV23_01600"
     CDS             2572..2922
                     /locus_tag="PKV23_01600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX84156.1"
                     /translation="MITKSLIAAAAVAVSLTALSPVQQAQAKVNVDINLGLGGGYYPG
                     YGYGGYYPVYEPYYGGITCNKGRKIVDWSGFHNVHAIDCSKPVYQYHAWKNGHKFRVK
                     VGMNGNIFGVKKLF"
     gene            3108..3467
                     /locus_tag="PKV23_01605"
     CDS             3108..3467
                     /locus_tag="PKV23_01605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX84157.1"
                     /translation="MAAGLAAAFSLSPQVAEAKVKVHIDIWQPGGYCYYNHDPFRCGG
                     YGYYPRPGYFVPHPVYRGKVSCGAAQWKLRNQGYKQINATDCGGRSYAFIARKNGVKY
                     RIKFDSYRNRITSRQPI"
     gene            3493..4125
                     /locus_tag="PKV23_01610"
     CDS             3493..4125
                     /locus_tag="PKV23_01610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX84158.1"
                     /translation="MRLQIIGLAALAAIAGSFATVADAEAGSRWRFLNPGYYFYDPPP
                     RDFYAYEYDDEEFYGDEDRPLAYYDPDDDYYDPRYYEPRYEGEEPVYIAPKKKTKKTV
                     KADPVPKPVAKKSVTAAQKKALTKTETTSVASVEKKAVAPAEKKTAAAAGGMSCEKAE
                     KIVSGYGFTSVKPTACSGQVYAFNATRSGKSYVIKLSSASGELTEVKKVQ"
     gene            4166..>4927
                     /locus_tag="PKV23_01615"
     CDS             4166..>4927
                     /locus_tag="PKV23_01615"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQX84159.1"
                     /translation="MELISTPENPVPRGAECTRVVTNDGFHLRAMSVRVSNPRGTVVI
                     LGGRADFMERYFETARDLMERGYCVASVDLRGQGGSQRLTEKRLRGHVGSFEEYDEDI
                     RAFMTQVVLPDCPPPYYVLAHSTGGNIILRLIRTRNWFKKCVLIAPLVDLNYGAWPIP
                     LVHLLTSIAMTLRLGWVYLPGKRQAPLGRKDFVGNPLTQDQRRWNRDSAVLEKAPQLG
                     SGGPTYGWLSAALKSIATLRKMKSEKGLKCPVLIVA"
BASE COUNT         1091 a         1428 c         1355 g         1053 t
ORIGIN      
        1 cggtaaaccg tttcgcgcgg catgaagagg tagcccacgc cataggtgtg gggtgcgggc
       61 aactcaaagc cgaggcgctt ggcttcctcc gagaaaaatt tatgggggat ctggatcatg
      121 atgccggcgc cgtcgccagc cttggggtca gcccccaccg cgccccggtg ttcgagattc
      181 ttcagaatgc tcaggccatc atcgatggtg cggtggctct tgatgttgtt gatgttggcg
      241 ataaggccaa tgccgcaggc atcatgctcg ttccggggat ggtacagccc atgcggttcg
      301 cgcatcccct ccggcagcat atgaagtgac tggctcatgg ctatcgtcct cttccaacat
      361 tccattcagc cccgggggct gtcattccgt ccttgtctct ggatttgatc tcttgagggg
      421 gcttttggtg ccgccggagc ctcatgcaag ggatcaaact ctgagagcgt ctttccgccc
      481 gagccgaccc ttaagtgcgt ttagtggtct gggcgcgggc catgcaaacg gcatcggcgg
      541 ctgtttttgc cgtcgctttc agatgtgttg tggtcgtatt ccggttcatc cgggctcaca
      601 agtccaaaat tgccatttat aaaggacagc catactgtcc tttcttcaaa atacacgatc
      661 tggagctttg cgcaaggaag gattcgtttg gagcaaaaat acgcaataaa attacgcaaa
      721 taatttcaag gccctcgacc atcccgcctc gaaacggtag ggaagagctc cccctttgga
      781 gctctcccat gaattttgca tctgataatc actacggcgt tcatccccgg attctggaag
      841 ccatggtgga agcgaacgct ggctcggctc cggcctatgg cggcgatgac tggactaaaa
      901 aagctgagtc gcagctttcc aagattttcg agaaggaagt gcgctccttc ctggtgacaa
      961 ccggcacagc agccaatggc ttggccctct cggccctaac cccgccctat ggcgccgtga
     1021 tctgccatgg cgaggcccat atcagcgttg atgaatgcaa ttccccggaa atgtttaccg
     1081 gcggcgccaa gatcctcggc cttcacgggc ccgccggaaa aatcacccct gccatggtcg
     1141 aaaagactct caaaggcttc atccgcggcg agcacgatcc caaacccgcc gccgtttcgc
     1201 tgaccaattc caccgaactg ggcaccacct attctccgga tgacgtgaag gcgatttcaa
     1261 atctcatcaa gccgcgcggc atgaaacttc atatggatgg cgcgcgcttt gccaatgcgg
     1321 tcgcaggctt gggcgtcacc ccggcggaac tcacctggaa atccggcgtc gatgccctgt
     1381 ccttcggcgc caccaaaaat ggcgccatga tgctggaagc ggtggttttc ttcgatacga
     1441 atttggccga tgatttcatc tatcgccgca tgcggggcgg ccagctcgtt tcaaaaggcc
     1501 gctatttggg cgggcaaatg ctggcttatc tcaaggacgg cctgtggctg gaaaacgcca
     1561 ggcaggctaa tggccttgcc gcacagcttg ccgaaggcct cggcaaattc aaaaacatcc
     1621 gcattcccaa tccggtgcag gccaacgaag tattcgccgt catgccgcgg gccctgaacg
     1681 acaagcttct ggcgtcaggt gctaagttct atgactggat gccagacagc cttggcaagg
     1741 ccattgccga cgacgaaatt tttgtccgct tcgttctgtc ctacgcaacc cccgaagccg
     1801 atgtccggaa attcctggac ttggccaagg acctgcaatc ctgattcaat aataaaaagg
     1861 ggcgcccgca aagagcgccc ccaatttgga attaggcggc ctttttagcc tcaagcactt
     1921 tctgctcatt cgcagtgcga atggcaatcg tgcgtggctt caaagcttcc ggaatttcgc
     1981 gcttcagcga aatatgcagc aggccatggt tcaaatcagc cccggtcact tcaacgtggt
     2041 cggcaagctg gaagcggcgc tcaaaattgc gggccgcgat gccctggtgc aggtattcgg
     2101 tcttttccgc gggtttttca gcctttttgc cggtaaccgt aagggcattg cccttcactt
     2161 cgagattgat gtcggaagcg ccaaagccgg caatggcgat ggtgatgcgg tattcgtcct
     2221 cgccaacccg ctcgatgttg tagggcggat aagccggggt ttcgaagctt gcagcatcat
     2281 caagcatccg ggcaagatgg tcaaaaccaa cactggagcg ataaagtggc gtaaaatcaa
     2341 acatttacat tctcctaaag caatgcattc agcgcggacc cacaattggc atccgctctg
     2401 gaaaaagtaa tttccgtttc cgggaatgca agggggaggg gccaaaaaaa atgaactgaa
     2461 aatgaatgat gggttctgac cccgttcaag ttcaggggtc tagtttcggt ctcaaggatg
     2521 gccaatcggg tcatcacaac gaaacaaaaa cttaaaccct ggagaacgaa aatgatcacc
     2581 aagtccctca tcgctgcagc cgctgttgct gtttctctta ccgccctgtc gccggttcag
     2641 caggctcagg cgaaagttaa cgtagacatc aacctcggcc tcggcggcgg ttattatcct
     2701 ggctacggct acggcggata ctatcccgta tatgagccct actacggcgg catcacctgc
     2761 aataagggcc gcaagatcgt tgactggtcc ggatttcaca acgtgcatgc catcgactgc
     2821 agcaagccag tttaccagta ccatgcctgg aagaatggcc acaagttccg cgtcaaggtc
     2881 ggcatgaacg gcaacatttt cggcgttaag aagcttttct aatccaacac cctgcaccgg
     2941 aacgcccgca aggcgttccg gcccctttgc cttgtttctg ccatgaagtt gaacggcaga
     3001 tgaacggggc tatcggcttt tgttcagcaa gattttgcca tgatcccgtc agataagctg
     3061 gggagcttgt gaagttggag ggcggaacga tgctcaggaa atttttgatg gcggctggcc
     3121 tcgcggcggc attttcactt tctccgcagg ttgcggaagc caaggtaaaa gtgcacatcg
     3181 atatctggca gcccggcgga tactgctact ataatcacga tcccttccgc tgcggcggtt
     3241 atggctacta tccccgtccg ggatatttcg tcccccaccc agtatacagg ggcaaagtgt
     3301 cctgcggcgc agcccagtgg aaactccgca accagggtta caagcagatt aatgcaacgg
     3361 actgcggcgg cagatcctat gccttcatcg cccgaaaaaa cggcgtgaag tacaggatca
     3421 agtttgattc ctaccgcaac cgcattacgt cgcgccagcc catctgattt gccaaaccga
     3481 aggaacagtc ccatgcgcct gcaaattatt ggcctcgccg cgctggccgc aattgccgga
     3541 tcattcgcaa cggtcgctga cgcggaggcg ggttcgcgct ggcgtttcct gaatcccggc
     3601 tattacttct acgatccgcc gccccgtgat ttctacgcct atgaatatga cgacgaggaa
     3661 ttctatggcg acgaagaccg gccgctcgcc tactatgatc ccgacgacga ttattatgac
     3721 ccgcgctatt atgagccgcg ctatgaaggc gaagagccgg tctacatagc cccgaagaag
     3781 aagacgaaaa agaccgtcaa ggctgacccg gtgcccaagc ccgtggccaa gaaatccgta
     3841 actgccgcgc aaaagaaagc attgaccaag actgaaacaa cctccgtggc ttcggtcgaa
     3901 aagaaagccg tggccccggc tgaaaagaaa accgccgccg cagccggcgg catgtcctgc
     3961 gaaaaggccg aaaaaattgt ctcgggctac ggctttacaa gtgtgaagcc aaccgcctgc
     4021 agcggccagg tttacgcctt caacgccacc cgcagcggca agagctatgt gatcaagctc
     4081 agttccgcca gcggcgagtt gaccgaggtc aagaaggttc agtaattctt gcccatgatg
     4141 ggcctctgct ttagagcagg ggcccatgga actgatttca acgccagaga atccggtgcc
     4201 gcgcggtgcc gagtgcacgc gcgtggtgac aaatgacggc tttcacctgc gcgccatgtc
     4261 ggtccgcgtc agcaatccca ggggcaccgt ggtcatcctc ggtggccgcg ccgatttcat
     4321 ggagcgctat ttcgaaaccg cgcgcgattt gatggaaaga ggctactgcg tggcctcggt
     4381 cgatctgcgc gggcaaggcg gctcccagcg gctgaccgaa aagcgcctgc gcggccatgt
     4441 cggcagcttc gaggaatatg atgaggacat ccgggccttc atgacccagg ttgtcctgcc
     4501 cgattgcccg ccgccctatt atgtgctcgc ccattccacc ggcggaaaca ttatcctgcg
     4561 ccttatacgc acccgcaact ggttcaagaa atgcgttctc attgccccgc tcgttgactt
     4621 gaactacggg gcctggccca tacccctggt gcatctcctc acctccatag ccatgaccct
     4681 gcgcctggga tgggtttatt tgccgggcaa aaggcaggct cccctggggc gcaaggattt
     4741 cgttggcaac cccctgaccc aggatcagcg gcgctggaac cgtgacagtg cggtgctgga
     4801 aaaagccccg cagcttggtt cgggcgggcc aacctacggc tggctctcgg ccgccctcaa
     4861 gtcgattgcc acgctgcgca aaatgaaaag cgagaaaggc ctgaaatgtc cggtcctgat
     4921 cgtcgct
//
LOCUS       DAORVN010000049         4884 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_12330_length_4884_cov_3.273556, whole genome shotgun sequence.
ACCESSION   DAORVN010000049 DAORVN010000000
VERSION     DAORVN010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 4884)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4884)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4884
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12330_length_4884_cov_3.273556"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            137..763
                     /locus_tag="PKV23_01620"
     CDS             137..763
                     /locus_tag="PKV23_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HQX84160.1"
                     /translation="MIDYLTSALATLLVVADPIFLSALFLGLEASLSAQERREVALRG
                     SIIAFCILVAAGLGGAKLLDLLGISLSAFRIAGGLLLLSAAAEMVFDRRSQRLQATAD
                     TAVSVDHVKNIAAFPLAIPLMAGPGAITAMILLAGKAGGQIELLGGLFGITALVMLAC
                     YLSFLMAERISKTMGVTGRAVISRLLGIILAALAVQFVIDGVLALANP"
     gene            complement(1239..2432)
                     /locus_tag="PKV23_01625"
     CDS             complement(1239..2432)
                     /locus_tag="PKV23_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007873520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HQX84161.1"
                     /translation="MKNQSHLLSITRPAQAEPDSGIVKAAMYGFGRPGVIPLWAGEGN
                     LPTPEAFCKPAAESLLKGETFYTWQRGIPELREALARYHTRHFGRDFNSENFFVTGGG
                     MQAIQTAIQMIAGEDDEVIIPSPAWPNYAGPLRLQGSRPIEVPMEFRNGKWSLDFDRL
                     FAAITPRTRAICLNSPSNPLGWTASRDDLIVIRDVCRKHGIWMFGDEVYSRFYYGAKN
                     EARAPSFLDVCDVEERLLLANTFSKNWAMTGWRVGWLQAPRELGPVIERIIQYNTSGT
                     AAFLQRGCVAALDDGDEFIAMQVTRALANRDLVVNFLREFPRIRFEVPTGAFYMFFKI
                     EGMTDSAATARRLIDEAGVGFAPGSAFGADGEGFMRMCYLRDPAQLQEALNRFGQWLK
                     TNHPK"
     gene            complement(2504..2923)
                     /gene="mscL"
                     /locus_tag="PKV23_01630"
     CDS             complement(2504..2923)
                     /gene="mscL"
                     /locus_tag="PKV23_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018687555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HQX84162.1"
                     /translation="MLEEFKNFAFKGNAFDLAVGVIIGTAFGKIVASIVDDLIMPVIG
                     AIFGGLDFNNYFFGLSSDVTAPTLEAAKAQGATFAYGSFITVLIQFLIIAFILFQLVK
                     ISNRLKKAEKPAPPPPASTTEVLLKEIRDALVTARKK"
     gene            complement(2951..4282)
                     /locus_tag="PKV23_01635"
     CDS             complement(2951..4282)
                     /locus_tag="PKV23_01635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HQX84163.1"
                     /translation="MKSSITLMPVSHRSVLTIAVPIMLSNVTEPLIGVVNTTVIGQLP
                     EAHYIGAIAIGSLIFSFLFWGFGFLRLSTGGLSAQAVGAGDSAEVIAVLLRALAIAVT
                     VGLCLIIASPLIEKVAFDLMGGSPEMRAQGEIYFRYRILSAPFALTNFCILGWFIGQG
                     RAAIAFVVQIFLNLVNMALGAWFVLSLGMTADGVGLSALIAEISAVLLGGILIWRTVH
                     KTKAWASWAQIWDREKVLRTLSANGDIMIRTLCLVFAFAWFAARGARAGDVTIAANAV
                     LLHFFEVAAYLIDGFAYASEALVGQSVGAKNRARFNRAIRLTTLWALVVGTICSAIIW
                     FAGPWFIDLMTVNPEVRETARHYLFWTALTPLLGTICFQFDGIFTGAMATRDMRNMMV
                     LSLMIYLASWWVLERNFGNHGLWAALSVFFIARGVTYAMRMPALSRVAFSS"
     gene            4330..>4884
                     /locus_tag="PKV23_01640"
     CDS             4330..>4884
                     /locus_tag="PKV23_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009796450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HQX84164.1"
                     /translation="MSQLPQKFQDWFAGRGWSLRAHQLAILEHAMHGESALLISPTGG
                     GKTLAGFLPSLVELSEAHERKGIHTLYISPLKALAVDIARNLEAPVLEMGLPITIETR
                     TGDTPHSRRKRQREHPPDILITTPEQVSLLIADAGAAHMLRHLKAIILDELHSLVTSK
                     RGVLLSLALARVHSIAPEARRIGLS"
BASE COUNT         1079 a         1326 c         1531 g          948 t
ORIGIN      
        1 aaagcggcga gatgcgcggc agcaatccgg tgttcgcgcc caaggaccgg tcaaacttcc
       61 tgcaggggct ggaaaatgcc atccaggttg gcaagcggtc tggcccgtga accctgcccc
      121 gtgttagcct gcgccgatga ttgattatct cacctcggcc ctggccaccc tgctcgttgt
      181 cgccgacccc attttcctca gcgcgctgtt cctcggcctc gaggccagtc tttcggcaca
      241 ggaacggcgc gaagtggcgc tgcgcggttc catcatcgca ttttgcatcc tggtggcagc
      301 ggggctcggc ggagcaaaac tgctggacct gctgggcatt tcgctttcgg cttttcgcat
      361 cgccggcgga ttgctgctct tgtcggcggc agcggaaatg gtcttcgacc gccgcagcca
      421 aaggcttcag gctacggcgg atacggcggt gagcgttgac catgtgaaaa acatcgcggc
      481 gtttccgctt gccattccac ttatggcggg gccgggtgct atcaccgcca tgattttgct
      541 ggcgggcaag gccggcgggc agattgagct gctgggcgga ctctttggga tcacggcgct
      601 tgtgatgctt gcctgctatc tcagctttct catggccgag cggatttcaa agactatggg
      661 ggtgacggga cgggcagtca tttcccggct gctcggaatc atcctcgccg cccttgcggt
      721 gcaattcgtg atcgacgggg ttctcgcgct cgccaacccg tagtgggact ataaacttat
      781 aataggcttt taaacgtgca tggaagaccc tacagcgggt ttcagggcgg gagagctggg
      841 tctatagcgg acaagggcag attcccgctt acgggcctgc cagatgcctt aggattaatt
      901 tctatttaag gttttaaaat ccatgacgca ccgggtgcgg gtggaaaaaa gaggggtgaa
      961 cggcagcaac ctttagccgc ccaccccttt tacgaagatg gcttcaaaag aaaccacccc
     1021 gcgacacgag tgctctgtaa tgaaaatggg aggatccaag acagacgtgt gcattgcaac
     1081 atactttgca aaggctgggc cagtttgttg gaggtctctt gtatttcgtt aaatatttga
     1141 atttgtatga taaaataaat tgcacaatct tctgttgagc atttgctcaa gccgctaagc
     1201 aaaaaatatg tgcaatgcag caataaaaga acagcaaatc atttgggatg attggttttt
     1261 agccactggc caaagcggtt caacgcttcc tgcaactggg ctggatcgcg gaggtaacac
     1321 atgcgcatga aaccttcgcc gtcggcgcca aaggccgagc ctggggcaaa gccaacgcct
     1381 gcctcatcga tgaggcgacg ggcggtggcg gcggaatccg tcatgccctc aattttgaag
     1441 aacatgtaga aagccccggt ggggacttca aagcggatcc gggggaattc gcggaggaaa
     1501 ttcaccacga gatcgcggtt ggccagagcc cgggtgacct gcatggcaat aaattcgtca
     1561 ccatcatcga gggcggcaac gcagccgcgc tgcaggaaag cggcggtgcc gctggtgttg
     1621 tactggatga tccgttcaat caccgggcct aattcccgcg gggcctggag ccagcccaca
     1681 cgccagccgg tcatggccca gttcttggaa aatgtgttgg cgagcaggag acgctcctcg
     1741 acgtcgcaga catcaagaaa ggagggagcg cgggcttcgt tcttggcgcc atagtagaag
     1801 cgggaataga cctcatcgcc gaacatccag atgccatgct tgcggcagac atctcggatg
     1861 acaatgagat catcgcgcga ggcggtccaa cccaggggat tggatggtga attgaggcaa
     1921 atcgccctgg tgcggggcgt gatggcggca aagaggcggt caaaatcaag ggaccacttt
     1981 ccattgcgga attccatcgg cacttcaatg gggcgcgagc cctgcaggcg caggggcccg
     2041 gcgtaattgg gccaggcggg ggacggaatg atcacttcat catcttcccc ggcaatcatc
     2101 tgaatggcgg tctggatcgc ctgcatgccg ccgccggtga cgaagaagtt ctcagaattg
     2161 aaatcgcggc caaagtggcg ggtgtggtag cgggccagcg cttccctcag ttccggaatg
     2221 ccgcgctgcc aggtgtaaaa ggtttcgccc ttgaggaggc tttcggcggc aggcttgcag
     2281 aaggcttccg gggttggaag attcccctct cctgcccaga gcggaatgac gccgggccga
     2341 ccgaagccat acatggcggc cttgacgatc ccgctgtcgg gttcggcctg ggctggtctg
     2401 gtgatgctca agagatgtga ctggtttttc ataggcgtcc ttagagcaaa ggaagggccc
     2461 ggatgtccag cacccgggcc catatcagtc atgccaaaga cgcttacttc tttctggcag
     2521 tcaccagcgc gtcgcggatt tccttgagca ggacttcggt ggtcgaagct ggcggcggcg
     2581 gggctggctt ctcggccttt ttcaaccggt tcgaaatctt gacaagctgg aagagaatga
     2641 aggcaatgat caggaactgg atcaggacag taatgaaact gccataggca aaggtggcgc
     2701 cctgggcctt tgccgcctca agtgtcggcg ccgtgacatc ggaggaaagc ccgaagaaat
     2761 aattgttgaa gtcgagtccg ccaaatatgg caccaatcac cggcatgatc agatcatcaa
     2821 cgatcgaagc gacgattttt ccaaaggcag tgccgatgat gacaccgacg gcgaggtcaa
     2881 aggcattgcc tttgaaggca aaattcttga attcttccag catcttaatt ccctttctta
     2941 atttcgctta tcaacttgaa aacgcgactc gcgatagtgc tggcatccgc atagcataag
     3001 tcacgccgcg ggcgataaag aaaacactga gtgcggccca gagcccgtga ttgccgaaat
     3061 tgcgttctag aacccaccaa gatgcaaggt aaatcatgag tgacagcacc atcatgttgc
     3121 gcatgtcgcg cgttgccatg gcgccggtga aaatgccgtc gaactgaaaa cagatggtgc
     3181 cgagcagcgg ggtgagtgcg gtccaaaaaa gatagtgcct tgctgtttcg cgcacctcgg
     3241 gattgaccgt catcagatcg atgaaccaag gtccggcgaa ccagatgatg gcgctgcaga
     3301 tggttcccac aaccagggcc cacagggtcg tgagcctgat ggcgcgattg aagcgggcgc
     3361 gatttttggc gccgacggac tggcccacca gggcctcgga ggcataggcg aagccatcga
     3421 tgagataggc ggccacctca aagaaatgca gcagcacggc gttggcggca atggtcacat
     3481 cgccggcgcg ggcgccgcgc gccgcgaacc aggcgaaggc gaacacgagg cacagggtgc
     3541 ggatcatgat gtcgccattg gcgctgaggg tgcgcaggac tttctcacgg tcccagatct
     3601 gcgcccaact cgcccaggct ttggtcttgt ggacagtgcg ccagatgaga atgccaccga
     3661 ggaggacggc cgagatttcg gcgatcaggg cggaaaggcc cacgccatcg gcggtcatgc
     3721 caagcgagag cacgaaccag gcgccgagcg ccatgttcac aaggttgaga aagatctgga
     3781 cgacgaacgc gatggcggcg cggccctggc cgatgaacca gccgaggatg cagaaattcg
     3841 tgagggcaaa gggtgccgac agaatgcggt agcggaagta gatttcgcct tgggcgcgca
     3901 tttccggcga accgcccatc aggtcaaagg ccactttctc gatgaggggg ctggcgataa
     3961 tcaggcagag gccgacggtg acggcaatgg ccagggcgcg caggagaacg gcgatgacct
     4021 cggcactgtc gccggcgcct acggcctgcg ctgaaaggcc gccggtggag aggcgaagaa
     4081 agccaaagcc ccagaacagg aaagagaaaa tcaacgagcc aatggcaatg gcgccaatat
     4141 agtgggcctc gggcaattgc ccgatcactg tagtattgac cacgccgatc agcggttcag
     4201 taacattcga caacatgatc ggcacggcaa tggtgagcac cgaacggtgg ctgaccggca
     4261 tgagcgtgat ggaggatttc ataagccccg tgttgcttta gcgtacccgc cgagtcaagc
     4321 tcagcgaaga tgagccaatt gccacagaaa tttcaggact ggtttgcggg gcggggctgg
     4381 agtctgcggg cgcatcagct tgcaattctg gagcacgcga tgcatgggga aagcgcgctg
     4441 ctcatttcgc caacgggcgg cggcaagacc cttgccggat tcctgccgag ccttgtggag
     4501 ctttccgagg cgcatgagcg caagggcatt cacacgcttt acatttcgcc gctcaaggca
     4561 ctggcggtgg atatcgcgcg caaccttgaa gcgccggtgc tggagatggg gctgccaatc
     4621 accattgaaa cgcgcaccgg cgacacgccg cattcgcgac gcaagcgcca gagggaacat
     4681 ccgcccgaca ttctgatcac cacgccggaa caggtttcgc tgctcattgc cgatgcgggg
     4741 gcggcgcata tgctgcgcca tctcaaagcc atcattctcg atgagctgca ttcgctggtg
     4801 acctccaagc ggggcgtgct gctgtcgctg gcgcttgcgc gggtgcacag cattgcgccg
     4861 gaggcgcggc gcatcggcct ttca
//
LOCUS       DAORVN010000050         4882 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_12346_length_4882_cov_3.727367, whole genome shotgun sequence.
ACCESSION   DAORVN010000050 DAORVN010000000
VERSION     DAORVN010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 4882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4882
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12346_length_4882_cov_3.727367"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(40..1101)
                     /locus_tag="PKV23_01645"
     CDS             complement(40..1101)
                     /locus_tag="PKV23_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019861306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HQX84165.1"
                     /translation="MTTTAQFLPAPPLLSPVAHNWKLALLAGLVVSVIAIVLFPARLS
                     FSVAGAMAALLAAAFAVNAYRRRYYGGLLVAPAIAVLFVMNIFPLLWSLGLSFFAYQS
                     NNQSIRFVGLNNYIKVLTNDIAAPANWDALINTGMFVVFTVALQMITGFLLAMLFAKQ
                     FPLRKYLLILVLTPMMLSVVASGVFFSYYYDPTFGILSYVMGEITGNQFILMDSKVGA
                     VAGIVFTDAWMWAPFVMLLVLAGLVSVPKYLYEAAAIDRVSKARQFWTITFPYIRGLL
                     MLALLFRTIEAFKLYDLVYILIRGGQDTKTIAYLITEIANEQNKTSEGAALSYVILFM
                     VIVLTNLYLYLANRRNQGA"
     gene            complement(1165..2586)
                     /locus_tag="PKV23_01650"
     CDS             complement(1165..2586)
                     /locus_tag="PKV23_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019861307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HQX84166.1"
                     /translation="MTKMKGVATAFSFAALMSVGGASLASAEDVTLTMAVPDWPPTRI
                     MKKFFDEQYKPKSGNTVKLEVDFIPWPDFYTRVNASLTSGEQKYNFIVSDSQWLGAFV
                     EGGYFRKINDLLDADPEFMATFKTIHPNPLNAYSTYPHKTENYYGFPQFPDVLVNYAR
                     QDIICHDEEQKNFQAKYGKKLPCTGEELDAMTWDDFKNVGEFFQRKKGDMLAGAPADD
                     DFYGIAYQAGKGYDFSSMQINGFIWQMGGDTWDETKAPKGQAEGVVNSPAAVAALEKY
                     LSLIQYMPPVAKTGTMDIFVSDQLFREGKVAMNIDWIGLGEASLAADSKVKDKLVFGQ
                     MPGTMGADGKMVRWSNIGGQPFVLTTWTTESQTKEAVDFVKWWLSNDIQHQFAQGGGQ
                     SAVKAVYEDPKYVTYRPWNRAWAPQLDWQKDVWHVPQFFELLTQQQDQFDLAITGKQD
                     AKTTLDNIAKFQQDKLKEVGLIE"
     gene            complement(2643..3734)
                     /locus_tag="PKV23_01655"
     CDS             complement(2643..3734)
                     /locus_tag="PKV23_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010911634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQX84167.1"
                     /translation="MAEDVKPAVRLSGVAKSYGNVRALKNLSFDVAAGRFFVLFGPSS
                     VGKTTTLRTIAGLVQPDSGRVEIDGRDFTQEPIAGRGVSMVFQSFALYPHLTVFENLA
                     YPLREAKVPSAEITRRVNETAAMLKLSHRLERKPNTLSGGEQQRVALGRSLVRQPKIL
                     LLDEPLTNLDAKLRHDMRAELKRLHRQFGMTIVYATPDELEALSMGEEIAVMRDGMVI
                     QRGTPEELYERPVDTYVAGKIGSPHMNLLQAKVGKDAKTFETPLGKMSPKVPRKTAAA
                     GEAAIIGIRPSDIRLAAKGEKAIASKVHLLEPLGDITIVSVETGKDVLRMVLPEAKAT
                     SMKVGDPASIYFDPDDIHIFRAASGQAMT"
     gene            complement(3871..>4882)
                     /locus_tag="PKV23_01660"
     CDS             complement(3871..>4882)
                     /locus_tag="PKV23_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018183181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HQX84168.1"
                     /translation="RDAGKAKTFAEQWGYESVETDWRKLVERKDIDVIDIASPNDTHH
                     DIAIAAAKAGKMVMCEKPLGRSAAESLKMVEAVEKSKVPNMVWYNYRRVPAVTLAKQL
                     IDEGKLGRIFHYRAKFLQDWTISKDLPQGGQGLWRLDVDVAGSGVTGDLLAHCIDTAM
                     WLNGGITKVSGMTETFIKERHHNITGKKEVVGIDDASLFLARFANGSLASFEATRYAR
                     GHKALYTFEINGENASIAWDLHDLHRLQYFDHRDEGRTRGWRSLHITDGDHPYMDKWW
                     VPGLQIGYEHTFIHQVADFLDAVGGGKVAAPTFRDALATDRVTDAVLKSARTGQWESA
                     ND"
BASE COUNT         1034 a         1343 c         1439 g         1066 t
ORIGIN      
        1 ccttccgaag gccgaatttt tctgcaagcg ttgccatgat tatgcgccct ggttccgtcg
       61 gttggccaga tagagatata ggttggtcag tacaatcacc atgaagagga taacgtagga
      121 gagagctgct ccctcactgg tcttgttctg ctcgttggcg atttcggtga taagataggc
      181 aatggtcttt gtatcctggc cgccacgaat gagaatgtag acgaggtcat agagcttgaa
      241 ggcctcaatg gttcggaaga gaagcgccag catcagcagc ccgcgaatgt aagggaatgt
      301 gatggtccag aattggcgcg ccttcgagac gcggtctatg gcggcggcct catagaggta
      361 tttcggcacc gagacgaggc ctgcaagcac cagaagcatg acgaaggggg cccacatcca
      421 tgcatcggtg aaaacgatgc cggcaacagc acccactttg ctatccatca agataaactg
      481 attgccggtg atctcgccca tgacatagct caggatgcca aaggttgggt catagtaata
      541 ggagaagaaa acgcctgatg cgacgacgga gagcatcatt ggcgtcagca cgaggatgag
      601 caaatacttc cgcaacggga actgctttgc gaaaagcatg gccagaagaa agcccgtgat
      661 catctgcagg gccaccgtga agaccacaaa catgccggtg ttgatcagcg cgtcccagtt
      721 ggcgggagcc gcgatgtcgt tggtgaggac cttgatgtag ttgttgaggc ccacaaaccg
      781 gatggattgg ttgttggatt gataggcgaa gaacgaaagc ccgagcgacc agagcaaggg
      841 aaaaatgttc atgacgaaaa gaacggctat ggccggcgcc accaacaggc caccgtagta
      901 tctgcggcgg taggcattta cagcgaatgc cgcggccagg agagccgcca tggctcccgc
      961 aacgctaaag gaaagccttg cgggaaacag gacaatggca atgacagaaa caacaaggcc
     1021 cgcaagaagg gccagtttcc agttatgagc caccggcgag agaagcggcg gagcaggcaa
     1081 gaattgcgcg gtggttgtca tggtactctt gtttcaaggt gaaaaaattg gggcgcagga
     1141 aaggcctgcg ccccaacaga cggattactc gatcaatcca acttccttca gcttgtcctg
     1201 ctggaacttg gcgatgttgt cgagggtggt cttggcgtcc tgcttgccgg tgatggcaag
     1261 gtcgaactgg tcctgctgct gcgtcaacag ttcaaagaac tgcggcacat gccagacgtc
     1321 cttctgccag tcgagctgcg gagcccaggc gcggttccag ggacggtagg tcacgtactt
     1381 gggatcctca tacacggcct tcacggcaga ctggccaccg ccctgcgcga actggtgctg
     1441 gatgtcgttg gacagccacc atttcacgaa atcgacggct tccttggtct gggattctgt
     1501 cgtccaggtt gtcagcacga atggctggcc accgatattg gaccagcgga ccatcttgcc
     1561 gtcagcgccc atggtgcctg gcatctggcc gaagaccagc ttgtccttga cctttgagtc
     1621 agcggcaagc gaagcttcgc ccaaaccgat ccagtcaatg ttcatggcca ccttgccctc
     1681 gcggaacagc tggtcggaca cgaagatgtc catggtgccg gtcttggcca ccggcggcat
     1741 gtactggatc aggctcagat atttttcgag cgccgcaaca gcggcaggag agttcacaac
     1801 accttcagcc tggccttttg gcgccttggt ttcatcccaa gtatcgccgc ccatctgcca
     1861 gatgaagccg ttgatctgca tggacgagaa gtcgtagccc ttgccggcct ggtaggcgat
     1921 gccgtagaaa tcgtcgtcgg ctggagcgcc cgcaagcatg tcgcccttct tcctctggaa
     1981 gaattcgccg acattcttga agtcatccca agtcatggcg tcaagctctt cgcccgtgca
     2041 gggaagcttc ttgccgtatt tagcctggaa gttcttctgc tcttcatcgt ggcagataat
     2101 gtcctggcgg gcataattca ccagaacgtc ggggaactgc ggaaagccat aatagttttc
     2161 cgtcttgtgc gggtaggtgg aataggcgtt cagcggattc ggatgaatcg tcttgaaggt
     2221 tgccatgaat tccgggtcgg catccagaag atcattgatc ttgcggaaat agccgccttc
     2281 gacaaatgca ccgagccact gcgagtcgga aacgatgaag ttgtacttct gctcacccga
     2341 ggtgagcgat gcgttgacgc gggtgtagaa atccggccat ggaatgaagt caacttcaag
     2401 ctttacagtg ttgccgctct tcggcttgta ctgctcgtcg aagaacttct tcatgatgcg
     2461 ggttggcggc caatcaggca cggccattgt cagcgtcacg tcctcggcgg aggcaagcga
     2521 tgcgcctcca accgacataa gggcggcaaa gctaaacgcc gtggcgaccc ctttcatctt
     2581 cgtcattgta gtcatctcag gttttctccc attgttaccc ggtttgcttc tgtccttcgg
     2641 tgtcaggtca ttgcctgtcc acttgcggcg cggaagatgt gaatatcgtc tggatcaaaa
     2701 tagatgctgg ctggatcgcc aaccttcatg gaagttgcct ttgcttcagg aagcaccatg
     2761 cggagcacgt cctttccggt ctctaccgaa acgatcgtaa tgtcgccgag tggctccagc
     2821 aggtgcacct tggatgcgat ggccttttcg cccttggccg ccagacggat gtcgctggga
     2881 cgaatgccga tgatggccgc ttcgcccgcg gccgctgtct tgcgcggcac tttgggactc
     2941 attttgccca gcggggtttc aaaggtcttg gcgtccttgc cgaccttcgc ctgtagcaag
     3001 ttcatatggg gcgaaccaat cttgccggcc acataggtat cgaccgggcg ctcatagagc
     3061 tcttccgggg tgccgcgctg gatgaccatg ccatcacgca tcacggcgat ttcctcgccc
     3121 atggaaaggg cttcaagctc atcgggcgtg gcgtagacta tggtcatgcc gaactggcgg
     3181 tgcaggcgtt tcagttcggc ccgcatgtca tggcgcagct tggcgtcaag attggtgagc
     3241 ggttcatcaa gaagcagaat tttcggctgg cggaccaggg aacggccaag ggcaacgcgc
     3301 tgctgctcac cgcccgacag ggtgttgggc ttgcgctcaa ggcggtggga aagcttcagc
     3361 atcgccgcag tctcgttcac gcgccgggtg atttccgccg acggcacttt tgcctcacgc
     3421 agggggtaag cgaggttctc aaaaacggtg aggtgcggat agagcgcgaa agactgaaac
     3481 accatggata cgccgcgtcc ggctatcggc tcctgagtga agtccctgcc gtcgatttcc
     3541 acccggccgg aatcaggttg taccaggccg gcaatggtgc gcagcgtcgt ggtcttgcca
     3601 acggaactag gcccgaacag gacaaagaac cgtcccgccg ccacgtcaaa ggaaagattt
     3661 ttcagagccc gcacgttgcc gtaggatttc gcaacccctg acagccgcac cgcgggcttc
     3721 acgtcttcag ccattcccat tccctgtctg cccgtccggg ctatttttgg cagagactag
     3781 cctgtctctc tttatttgca agccgcctat catcaatcca aatcaattat tatgggcgca
     3841 cccggaattt tacgaacccc tgcccaacaa ttagtcattt gcgctttccc actggccggt
     3901 tctggccgat ttcaggacag catcggtaac gcgatcggtt gccagggcat cacggaaagt
     3961 cggagcggcg acctttccgc cgccaacggc atcgaggaag tcggcaacct ggtggatgaa
     4021 cgtgtgttca tagccgatct gcaatcccgg cacccaccac ttgtccatgt aggggtgatc
     4081 gccatcggta atatgaagtg aacgccagcc acgggtgcgg ccctcgtcac gatgatcaaa
     4141 atactgcagg cggtgcaggt cgtgcaggtc ccaggctatc gaggcatttt cgccgttgat
     4201 ttcgaaggtg tagagggcct tgtggccgcg ggcgtagcgg gtggcctcga aactggccag
     4261 agacccgttg gcaaagcggg ccagaaaaag gctggcatcg tcgatgccga cgacttcctt
     4321 tttgccggtg atgttgtgat ggcgttcctt gatgaaggtc tcggtcatgc cggagacttt
     4381 ggtgatgccg ccgttcagcc acatggccgt atcaatgcaa tgggcgagaa ggtcgcccgt
     4441 tacgccgctt ccggccacgt ccacatccag gcgccacagg ccctggccgc cctggggcag
     4501 gtccttggaa atggtccagt cctggaggaa tttggcacgg taatgaaaaa tcctgccaag
     4561 cttgccttcg tcgatcaact gcttggcaag ggtgacggca ggcacgcgcc ggtaattata
     4621 ccacaccatg ttgggcacct tggacttctc gacggcttcc accattttca gggattcagc
     4681 ggcggagcgg cccaggggct tttcgcacat caccattttt ccagccttgg cggcggcaat
     4741 ggctatgtca tgatgggtgt cattggggct ggctatgtcg atgacatcga tatccttgcg
     4801 ctcgaccagt ttgcgccaat cggtttcaac agactcatag ccccactgtt cggcaaaggt
     4861 ctttgccttg ccggcatcgc gg
//
LOCUS       DAORVN010000051         4878 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_12364_length_4878_cov_3.561891, whole genome shotgun sequence.
ACCESSION   DAORVN010000051 DAORVN010000000
VERSION     DAORVN010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 4878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4878
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12364_length_4878_cov_3.561891"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..495)
                     /locus_tag="PKV23_01665"
     CDS             complement(<1..495)
                     /locus_tag="PKV23_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008967699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQX84169.1"
                     /translation="MSLLQVNAVSKSFGAIHALTDVSFSLEAGEVVGFMGDNGAGKST
                     MVKLIAGNFPPTQGEVVVDGQICHFHKPIEARAKGIEVVYQDLALADNLTAAQNVFLG
                     REINKGVWPFNILDKEAMIRRAAELFSELKSETRPRDLVKKMSGGQRQAVAIARTRLS
                     NAKLV"
     gene            693..1883
                     /locus_tag="PKV23_01670"
     CDS             693..1883
                     /locus_tag="PKV23_01670"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HQX84170.1"
                     /translation="MNVQFARRMENVQPSAIRELLRLGADPSIISFGGGYPDASIFPL
                     EQLNAVYEEAIARNGQISLQYTVSNGIPKLREQIAQRMGRDGMACTAEDVLILQGAQQ
                     GLDLTAKLLIDRGDIIITENPTFLGALIAFNPYEPRYATVRIDENGMDMEDLERVLLA
                     TKNAKLLYTVPDFQNPTGVTMSEERRRRLIELANRFNLIILEDTPYREIRYQGKSLPT
                     LKSLDTEGRVIFVGSFSKILAPGLRLGWAVASPEIIQKLGLLKLAVDTQCSTLNMTAV
                     SLFLERFDIEQHIRLIRQTYLRKKELMLATIRQTFPQDIRFTDPSGGLFTWLTFPDGF
                     DAAKFMLEQSLPKAKVAYVPGATFFPLKQETNHARVSYSTQPDEIIVKGITALGQLLK
                     SELH"
     gene            1914..2597
                     /locus_tag="PKV23_01675"
     CDS             1914..2597
                     /locus_tag="PKV23_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015927922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylmenaquinone methyltransferase"
                     /protein_id="HQX84171.1"
                     /translation="MPVIIDNYQPEASSPGTIERWRQVPVAVAVDVSHAECQIDRAIR
                     PLCPPGQQPRLFGRAVTALCEPPDFGAVLYALDKIKPGDVLVIAAGGHADTAMIGEIL
                     GGHLRRLGACGLVCDGAVRDVAELAKWNDLPVFSRAITPRGPTSAERGRVNCSVVVGG
                     RLISPGDLVIGDDDGLASLDPKSIVKFIAEAEGKLAKEAEWQASLAARRTAAETFHLG
                     TPQAGEGSA"
     gene            2882..3907
                     /locus_tag="PKV23_01680"
     CDS             2882..3907
                     /locus_tag="PKV23_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018185466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HQX84172.1"
                     /translation="MLDDLIKEARTRRDVFKAIGATALAGALLADAGFDPAMAQALAP
                     SGKPMKAAFSNAGLQASWCAQGKQAAEAWAKLMNVEITWFDGELAATKQRAAIDNMAT
                     QKWDFVAIQTFGIGTLTDPIQKMIDAGTPVIAMDTLVAPPGTIAIHTFIAPDNEMMGS
                     AVTSQLMAAIGGKGNVVMTQGALGHSGAQGRAKGFKSVVANFPDVKVINEEPADWDVT
                     KVARIWDSVLTQNPDIAGAFFHNDDMALAANQVMKQKGREGIIIGGVDAMPPAVQAVL
                     DGTMLATVRNPSCRIHGWSVAAGVAAVQGGEKAGTDIPNYILADGPVITKETAPGHLW
                     LQKNFLI"
     gene            3972..>4878
                     /locus_tag="PKV23_01685"
     CDS             3972..>4878
                     /locus_tag="PKV23_01685"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018185467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HQX84173.1"
                     /translation="MSKSFGGIAALKDVDMTLQAGEIHGLVGENGAGKSTTMKIIAGV
                     YHDYDGEMRIDGTKVHFRSAREALAAGIGMVHQELSTVPDLTVAENVFLGHQPLTKMG
                     TVDWARINKEATRLIASLGLDIDPRTRMGSLPVGLQQLVELSRVLFSGARIIILDEPT
                     SALSPPEVKRLFDVLRKIKADGRGIIFISHFLDDVLEISDTVTVFRNGKKVATEAAAT
                     LTKDAVISHMIGRGSIGMHMGESADLTGDDSKPVVLAAEAISDGRQLRDVSLKLRAGE
                     ITGVYGFMGCGQIELARALFGKTRIK"
BASE COUNT         1017 a         1474 c         1462 g          925 t
ORIGIN      
        1 caccagcttg gcgttggata gccgtgtgcg ggcaatggcc acggcctggc gctggccgcc
       61 cgacattttt ttcaccagat cgcgcgggcg ggtttccgac ttgagctcgc tgaagagctc
      121 ggcggcgcgc ctgatcatcg cctccttgtc gagaatattg aaaggccaga cgcccttgtt
      181 gatttcgcgg ccgagaaaga cgttctgcgc cgccgtcagg ttgtcggcca gggccaggtc
      241 ctgatagacg acttcaatcc ccttggcgcg cgcttcgatg ggtttgtgga aatggcagat
      301 ctggccatcg acgacgacct cgccttgggt tggcggaaaa tttccggcga tcagcttgac
      361 catggtggat ttgccggcgc cattgtcgcc catgaagccc acgacttcgc cggcttcgag
      421 tgaaaaactg acatccgtca acgcgtgaat agcgccaaag ctctttgata ctgcgttaac
      481 ctgcagcaac gacatcaact gacctcccga attggacctc acggcttctc tgtccgctgg
      541 ccaaaaccga acgtagacgc ttcggcaaaa atttcaatcc ccttgagcct caggtccttg
      601 cacggaacat acgataagca gccctgttga cggcgccagc ggagaccgcc aaattggccc
      661 catctcatcg agatcaagag gaattcccca gcatgaatgt gcaattcgca aggcggatgg
      721 aaaacgtcca gccttcggcg atccgcgaac tccttcgttt gggggccgat cccagcatca
      781 tatcgttcgg cggcggctat ccggatgcgt ccattttccc gctggagcag ttgaacgcgg
      841 tttatgaaga ggccattgcg cgcaatggcc agatatccct gcagtatacg gtttcaaacg
      901 gcatcccgaa gcttcgcgaa cagattgccc agcgcatggg gcgcgatggc atggcatgca
      961 ccgcggaaga cgtgctgatc cttcagggcg cgcagcaggg gcttgacctc accgcgaaac
     1021 tgctgatcga ccgcggcgac atcatcatca ccgagaatcc cacattcctc ggcgccctca
     1081 tcgccttcaa tccttacgag ccgcgctatg ccacggtgcg catcgacgag aacgggatgg
     1141 acatggagga tcttgaacgt gtcctgctgg ccacgaaaaa cgccaagctc ctctacacgg
     1201 tccctgattt tcagaatccc accggggtca ccatgagcga ggagcggcgc aggcggctga
     1261 tcgaactggc gaaccggttc aatctcatca tcctggaaga tacgccctac cgtgaaatcc
     1321 gctatcaggg gaaatcgctc ccgaccctga agagcctgga tacggaaggc cgcgtcatct
     1381 tcgtgggcag cttttccaag attctggcgc cgggcttgcg gctgggctgg gccgttgcat
     1441 cgcctgagat cattcagaaa ctcggcctgc tcaagcttgc ggttgacacg cagtgcagca
     1501 cgctcaacat gacggcggtc tcactgtttc tggaacgctt cgacatcgaa cagcacatcc
     1561 gcctgatccg gcagacctat ctccgcaaga aagagctgat gctggccacg atccgccaga
     1621 ccttccccca ggacatcagg ttcaccgatc caagcggcgg attgttcacc tggcttacgt
     1681 ttccggacgg ctttgacgcg gcgaaattca tgctggagca aagcctgcca aaagccaaag
     1741 tcgcctatgt tcccggcgcc actttctttc ccctgaaaca ggaaacaaac cacgcccggg
     1801 tgagctattc gacacagccg gacgagataa tcgtcaaggg catcacggcc ctggggcagt
     1861 tgttgaaaag cgaactccat taaatcaaca tcacttcatg agggataagt gcaatgcccg
     1921 tcattattga taattatcag cctgaagcat cctccccggg cacaatcgaa cggtggcggc
     1981 aggtgccggt ggcggttgcg gtggatgtaa gccacgcgga atgccagatc gaccgcgcca
     2041 tccgcccgct gtgcccgccg ggccagcagc cgcggctgtt tggccgggcg gtgacggccc
     2101 tatgcgagcc gccggatttc ggcgcggtgc tctatgcttt ggataaaata aagccgggcg
     2161 acgtgctggt gatcgccgcc ggcgggcatg cggacaccgc aatgatcggc gagattctgg
     2221 gcgggcacct gagacgcctg ggggcctgcg ggctggtctg tgacggggcg gtccgcgatg
     2281 tggcggagct tgccaagtgg aatgatcttc cggtgttcag ccgggcgatc acgccgcgcg
     2341 ggcccacttc ggcagagcgc ggccgggtca actgcagcgt tgtcgttggc gggcggttga
     2401 taagtcccgg cgacctggtc atcggcgacg atgacggact tgcatcgctt gatccaaaat
     2461 cgatcgtgaa attcattgca gaggcggaag gcaagcttgc caaggaggcc gaatggcagg
     2521 ccagcctcgc cgcacgccgc acggcggccg aaaccttcca tctgggtaca ccgcaggcgg
     2581 gcgaaggctc tgcgtaagca tcctttttca ggtttgggtg caatgcaata tcaacttctg
     2641 atcaatcgtg aagcgatgga gatgcttaaa tccggtcact tcccgcgcgc gtcatagcca
     2701 tgaccgaaac ccgggcacgg caattttcat ttgtatgacg attggatttg acaggctggg
     2761 gagatgacgg caagatttgc agcggagggt gtcagcagac tcgtgcaata aaccgagact
     2821 caaacagcac gcgactcaaa aacactcctg cctaataaaa attcacatgg gagaatttca
     2881 tatgctcgac gaccttatca aagaagcaag aacccgccgc gacgtcttca aggcgatcgg
     2941 cgccacggcc cttgccggcg cccttctggc cgatgcggga tttgatccag ccatggcgca
     3001 ggccctggcg ccatcgggca aaccgatgaa agccgccttc tcgaacgccg gcctccaggc
     3061 ctcctggtgc gcccagggca aacaggctgc ggaagcctgg gccaagctga tgaatgtcga
     3121 gatcacctgg tttgacggcg agctcgcagc caccaagcag cgcgcggcaa tcgataacat
     3181 ggccacccag aagtgggatt tcgtagccat acagactttc ggcatcggta cgctcaccga
     3241 tccgatccag aaaatgatcg atgccggcac cccggtcatc gccatggaca cgctggttgc
     3301 ccctccggga accatcgcca tccatacctt catcgcgcct gacaacgaga tgatgggctc
     3361 ggccgtgacc tcgcagctca tggccgccat cggcggcaag ggcaatgtgg ttatgacaca
     3421 gggcgcgctc ggccattccg gtgcccaggg ccgtgccaag ggcttcaagt cggtcgtggc
     3481 caacttcccc gatgtgaaag tgatcaacga agaaccagcc gactgggatg tgaccaaggt
     3541 cgcccgcatc tgggattcgg tcctgacgca gaacccggac atcgccggcg ccttcttcca
     3601 caatgacgac atggcactgg ccgccaatca ggtcatgaag cagaagggcc gcgaaggcat
     3661 catcatcggc ggcgtggatg ccatgcctcc ggcggtgcag gcagtgcttg acggcaccat
     3721 gctggcaacg gtgcgcaatc cgtcctgccg tatccatggc tggtcggtgg ctgccggtgt
     3781 tgcggcagtt cagggcggcg agaaagccgg cacggatatc ccgaactata tcctggcgga
     3841 cggaccggtc atcaccaagg aaacagctcc gggccatctc tggctgcaga agaacttcct
     3901 gatctgacct gttcatgacg gctcaatcac ttagcggggg cggcttgccg cccctgcttc
     3961 agctttcaaa tgtgtcaaaa tcattcggcg gcatcgcggc gctgaaggat gtcgatatga
     4021 ctttgcaggc cggcgagatt catggccttg tcggcgaaaa cggcgccggc aaatccacca
     4081 cgatgaaaat catcgccggt gtctatcacg actatgacgg cgaaatgcgc attgacggga
     4141 caaaggtcca cttccgctcg gcgcgcgagg cgctggcggc gggcatcggc atggtgcacc
     4201 aggaactatc gacagttccg gacctgacgg ttgccgaaaa cgtttttctg ggccaccagc
     4261 cgctgacaaa gatgggcacg gtggactggg cccgcatcaa caaggaggcg acacggctga
     4321 ttgccagcct cggccttgat atcgaccccc ggacccggat gggttcgctg ccggtcggcc
     4381 tgcagcagct ggtggaactt tcgcgcgtcc tgttttccgg cgcccgcatc atcatcctgg
     4441 atgagcccac ctcggcgctg tcgccgccgg aggtcaagcg gctgtttgac gttctccgca
     4501 agatcaaggc ggacgggcgc ggcatcatct tcatttcgca cttcctcgac gacgtgctgg
     4561 agatttccga cacggtcacg gtattccgca acggcaagaa ggttgccacc gaagctgcgg
     4621 ccacgctcac caaggatgcg gtgatctccc acatgatcgg gcgcggttcg attggcatgc
     4681 acatgggaga aagcgccgac cttacgggcg atgacagcaa gccggtcgtg ctggcagccg
     4741 aggcgatcag cgacggccgg cagctccgtg atgtctcgct caagctgcgg gccggggaaa
     4801 tcaccggggt ctacggcttc atgggctgcg ggcagatcga gctggcccgc gccctgttcg
     4861 ggaaaacccg gatcaagc
//
LOCUS       DAORVN010000052         4779 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_12819_length_4779_cov_3.040008, whole genome shotgun sequence.
ACCESSION   DAORVN010000052 DAORVN010000000
VERSION     DAORVN010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 4779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4779
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12819_length_4779_cov_3.040008"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..386
                     /locus_tag="PKV23_01690"
     CDS             <1..386
                     /locus_tag="PKV23_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019964134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HQX84174.1"
                     /translation="SRFVDELPEAFIEVAPMSNNFGGHSLGGYGASRFDDQKPFTNTY
                     ATPGWQRAQERWSKGEGLRSTPKFIEGEMVTHGEENPSLAKGTRIFHQKFGYGRITSV
                     DGNKLTIDFEKAGEKRVLDSFVTAA"
     gene            465..2720
                     /locus_tag="PKV23_01695"
     CDS             465..2720
                     /locus_tag="PKV23_01695"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HQX84175.1"
                     /translation="MQIATEVRPAARAKADALNRAAPAVFTMAIFTSAFLLFAIQPMF
                     TKMILPQLGGSPAVWSVAMVFFQAVLLLGYLYAHLSTRYLPPRTAIYVHLGLLLVTFI
                     ALPISNAAGIGQPPAEGQALWLIGVFTFSVGLPFFAVAGNGPLLQAWFARTRHKDAGN
                     PYFLYAASNLGSFAALLLYPLIFETALRLPQQSLGWSIGFMALALMIAASGYVLQLTM
                     AEPKLGIIQPGPVAGPTKRRVGEYIWLSFIPSALLVAVTAHISTDIAPAPFLWVIPLA
                     LYLLTFVLIFRDQPLVPTTIVAHLVPALAGLIILSEVVLLNIFLSVALHLGFFLVVAL
                     MFHQRLYNLRPPAEHLTQFYLWMSFGGVLGGIFSGLLAPVIFDRILEYPILIGLVMLA
                     HPAVLKASRSDFAREALPVLGLALLVAAGIYLFREHEFISSSTYHRYLIAVASIVIIG
                     LFVHQVARAALIVAALLAVEISPAGFLDTTYERSFFGVHKIYLRDNGQFRALAHGTTL
                     HGAIRIKNPDGSKYSGPVTPLTYYHPQGVLAQTLALLPPQINGREIAVVGLGAGSHSC
                     NGMTRDRWTYFEIDPVVIKIARDPNQFEFLSGCTPDARIVLGDARLTLAEEPAPAFDY
                     LLIDAFSSDTIPIHLLTREAIELYVSRLKSDGILVIHISNRHLELQSVVAALARDARI
                     AIKTRVHITRSTSLAEPNSSAVAIFARQPQTLAPFTEELGWQQPPNNDVSVWTDDYSN
                     ILGAIWRKHSN"
     gene            complement(2728..3204)
                     /gene="msrB"
                     /locus_tag="PKV23_01700"
     CDS             complement(2728..3204)
                     /gene="msrB"
                     /locus_tag="PKV23_01700"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013891876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HQX84176.1"
                     /translation="MDRRSILKYAGFTAVGLVAGSRAGFAEEFEISKTEAEWKAVLSP
                     EQYAVLREEDTEAPNSSPLLDEHRKGIFACAGCDLPLYSSDTKYDSGTGWPSFYDVLT
                     NAVGKKEDNTFFSTRTEVHCRRCGGHLGHVFEDGPPPTGLRYCMNGLALKFVPATA"
     gene            complement(3251..4681)
                     /locus_tag="PKV23_01705"
     CDS             complement(3251..4681)
                     /locus_tag="PKV23_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013165784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQX84177.1"
                     /translation="MVSPARLLPLIVASALFMENMDSTVIATSLPAIAADLGTDPIVL
                     KLAFTTYLLSLTVFIPISGWCADRFGAKHVFRAAIVVFMLGSIACGYAQSLGWLIAAR
                     ALQGLGGAMMVPVGRLIILREVPKSQLVDALAWLTIPALIGPLVGPPIGGFITTYFDW
                     RWIFWMNIPIGLIGLMVANRFLPEMPPEPVQRLDVRGFLFSALGLSSLVFGFTIIGRD
                     FLPLYGQVLLIGLGAVCLALYVRHAATAKNPIIDLKLLRIPTFRAGVEGGSFYRIGVG
                     AIPFLMPLMFQLGFGLTAFQSGLLSCWAAAGAIAMKFSASALLRCFGFRQLLIVNGVV
                     SCLLMACYGLLSEATPFVLISLLLLVGGFLRSLQFTAMNALTYSDIDRGDTSNATTFY
                     AVAQQFSLSAGVAVAALVLEAVQAWRGEPVIASDDFSMAFFIVSGIAMTCVFYFARLD
                     ANAGSSVITRRRASAPERVVEGPVEL"
BASE COUNT         1020 a         1516 c         1271 g          972 t
ORIGIN      
        1 cctcgcgctt tgtcgacgaa ttgccggaag cctttatcga agtcgcgccc atgagcaaca
       61 actttggcgg ccacagtctc ggcggctatg gcgccagccg ctttgatgat cagaaaccct
      121 tcaccaacac ttatgccacg ccgggctggc agcgcgccca ggaacgctgg agcaagggcg
      181 agggcctgcg ctcaacccct aaattcattg aaggcgaaat ggtcacccac ggcgaggaaa
      241 accccagcct tgccaaaggc acccgtattt tccaccagaa atttggctat ggccgcatca
      301 cgtccgtcga tggcaacaag ctcaccatcg acttcgaaaa agccggcgag aagcgcgtcc
      361 tcgacagctt cgtgacggcg gcttgagcgg cctgatttga cttaatcccg tcttaagaaa
      421 acctccctag acctttcatt tggcggaatg gaaggcagca ttttatgcaa attgcaacgg
      481 aggtcagacc ggctgcgcgt gccaaagctg atgccctgaa ccgggccgca cccgccgttt
      541 tcaccatggc catttttacc tcggcctttc tgctcttcgc catccagccc atgttcacca
      601 agatgatcct gccgcagctc ggcggttctc ccgccgtatg gtccgtagcc atggtgttct
      661 ttcaggccgt gctgcttttg ggttatctct atgcccatct ctcaacccgc tacctgccgc
      721 cgcgtaccgc catatacgtg catctgggcc ttctccttgt aaccttcatc gctttgccga
      781 tctccaatgc cgcgggcatt ggacaaccgc cggccgaagg ccaggccctt tggcttatcg
      841 gcgtgttcac cttttccgtg ggcctgccat tctttgccgt cgccggaaac ggtccgctcc
      901 tgcaagcctg gttcgcccgc acccggcaca aggatgccgg caatccgtac tttctttatg
      961 ccgcttcaaa tctcggatcc ttcgcggcac tcctgctcta tcccctgatc tttgaaaccg
     1021 ccctgcgctt gccccagcaa tcccttggct ggtcaatcgg ctttatggcc cttgccctga
     1081 tgatcgcagc ctcgggctat gtcttgcagc tgaccatggc agaaccgaaa ctgggcatca
     1141 tccaaccggg acctgtggca ggaccgacga agcgccgggt cggcgaatat atctggctct
     1201 cgttcattcc gtcagccctg ctggtggcgg tcaccgccca catctcaacc gacatagcgc
     1261 cggccccatt tctctgggtg atcccgctgg ccctctatct cctgactttc gtgctgatct
     1321 tccgcgacca gccgctggtt cccacaacaa tagttgccca ccttgttcca gccctggccg
     1381 ggttgatcat tctatccgaa gtggttctcc taaacatctt tctgagtgtc gcccttcacc
     1441 tcggattttt ccttgttgtg gccctgatgt tccaccagcg cctttacaac ttgcgcccac
     1501 ccgccgaaca cctcacgcag ttctacctgt ggatgtcctt tggcggcgtt ctcggcggca
     1561 ttttctccgg gttgcttgca cccgtaattt ttgatcgcat cctggaatac ccgattctca
     1621 taggcctcgt gatgctggcc catccggcgg tgctcaaagc ctcacgaagt gatttcgcaa
     1681 gggaagcgct gccggttttg ggactggcgc tgctggtggc ggcggggatt tatctgtttc
     1741 gcgagcatga gttcatcagt tccagcacct accatcgata tctcatagct gtggccagca
     1801 ttgtgatcat cggtctcttt gttcatcaag ttgcccgcgc ggctctgata gttgcggcgc
     1861 tgctggcagt cgagatatcg ccagccggct ttctggacac gacttacgaa cgctcattct
     1921 ttggcgtcca caaaatttat ctgcgcgaca atggacagtt ccgcgcgctg gcgcacggaa
     1981 caaccctgca cggcgcaatc cggatcaaaa atcccgatgg gtcaaaatat tccggtccag
     2041 tcacgccgct cacttactat cacccccaag gcgttttggc gcagacactt gctttgctcc
     2101 cgccccagat caacggccgt gaaattgccg tcgtaggcct tggcgcaggc tcccattcct
     2161 gcaacggcat gacccgagac cgctggacgt attttgagat tgatcctgtc gtcatcaaga
     2221 tagccaggga ccccaatcaa tttgaatttc tgtccggctg cacacctgac gccaggatcg
     2281 ttctgggcga tgcgcgtttg acccttgccg aagaaccggc tcccgccttc gactacttgc
     2341 tcatcgatgc cttctcatcg gatacaattc cgatccattt gctgacccgc gaggccattg
     2401 agctttatgt atcacgcctc aagagcgatg ggattctggt catccacata tcaaaccgcc
     2461 acctggaact gcaaagcgtt gttgcggccc ttgcccgcga tgccaggatc gccataaaaa
     2521 cccgagtcca tatcaccagg tcaacctcgc ttgccgagcc gaattcttcg gcagttgcga
     2581 tttttgcacg ccagccgcag acccttgcgc cctttaccga agagcttggc tggcaacagc
     2641 cgccgaacaa tgatgtgtcg gtctggactg acgactattc aaacatcctg ggcgccatct
     2701 ggcgcaagca ttccaactga tgccggttca cgccgtcgcc ggcacgaact tcagcgccag
     2761 cccgttcatg caatagcgca ggcctgtggg cggcggaccg tcctcgaaca cgtggcccag
     2821 atgcccgccg cagcgccggc agtgaacctc ggtgcgcgtg ctgaaaaacg tattgtcttc
     2881 cttcttgccg acagcgtttg tcagcacatc ataaaagctc ggccagcccg tgccgctgtc
     2941 atacttggtg tccgaagaat agagcggcag gtcgcagccg gcgcaggcga aaatgccctt
     3001 gcggtgctcg tccagaagcg ggctgctgtt gggcgcctcg gtatcttcct cgcgtaagac
     3061 tgcatattgt tcaggcgaaa ggacagcctt ccattccgcc tcggtcttgc tgatctcaaa
     3121 ctcctcggcg aagcctgcgc ggcttcccgc gaccaggccc acggccgtaa atccggcata
     3181 tttcagaatg ctgcggcgat ccatgatatc ctcactttac cccaattacg ccccaaacag
     3241 gcaaaaggtt tcagagctca accggcccct ccaccacgcg ctccggggcg gaggcccttc
     3301 gccgtgtaat gaccgaagaa ccggcattcg catcaagcct cgcaaagtag aatacgcagg
     3361 tcatggctat tcccgaaacg atgaaaaacg ccatggaaaa atcatccgac gcgatgaccg
     3421 gctcgccgcg ccaggcctgc accgcctcca ggacaagggc cgcgacagcc accccggcag
     3481 acagcgagaa ctgctgcgcc accgcataaa acgtcgtggc attgctggtg tcgccgcggt
     3541 cgatgtccga ataagtcagc gcattcatcg ccgtgaattg caatgaccgg aggaagccgc
     3601 ctacaagcag caggagcgag atcaggacaa aaggagtggc ttctgaaagc aggccataac
     3661 aggccatgag caggcacgat accacaccgt taacgatcag caattgcctg aagccaaagc
     3721 accgcagcaa ggccgatgcc gaaaacttca tggctatcgc ccccgccgcc gcccagcagc
     3781 tcagcaggcc ggactggaag gcggtgaggc caaagccaag ctgaaacatg agcggcatga
     3841 ggaaaggaat ggcccccacc ccgatgcggt aaaagcttcc accctccacc ccggcacgga
     3901 aggtgggaat gcgcagaagc ttcaggtcaa tgatgggatt tttagcagtc gcagcatggc
     3961 gcacgtaaag tgccaggcaa accgcgccaa ggccgatgag aagcacctgc ccgtacagcg
     4021 gcaggaaatc ccggcctatg atcgtgaagc caaataccag tgacgaaagc ccgagcgccg
     4081 aaaacagaaa gccgcgcaca tcaagccgct gcacgggttc tggcggcatc tccggcagga
     4141 accggtttgc caccataagg ccgatgagcc cgattggaat attcatccag aatatccagc
     4201 gccagtcaaa ataggtggtg atgaacccgc cgataggcgg tcccaccagg ggaccgatga
     4261 gagccggaat ggtgagccag gccagggcat ccaccagttg tgattttggc acctcgcgga
     4321 ggatgatcag gcggcccacc ggcaccatca tggccccgcc gagaccctgc agcgcgcgcg
     4381 ccgctatcag ccagcccagc gattgggcat agccgcaggc gatggagccg agcatgaaca
     4441 ccacaattgc cgcgcgaaac acatgctttg cgccaaagcg gtcggcacac cagccgctga
     4501 tcggaatgaa aaccgtaagg ctgaggagat aagtggtgaa cgccagcttc aacacaatgg
     4561 ggtcggtgcc aaggtccgcc gcgatggccg gcagcgacgt ggcaatcacc gtcgagtcca
     4621 tgttttccat gaacagggca gaggccacga tgagtggaag caggcgggct ggggagacca
     4681 tttacatgaa ccggagaatg ccaaactatg gctgatgata caatgatagg agcccgtgaa
     4741 agttcatgcg taaatgcgca aaccccatgc aaccacctc
//
LOCUS       DAORVN010000053         4766 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_12882_length_4766_cov_3.321376, whole genome shotgun sequence.
ACCESSION   DAORVN010000053 DAORVN010000000
VERSION     DAORVN010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 4766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4766
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12882_length_4766_cov_3.321376"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..428)
                     /locus_tag="PKV23_01710"
     CDS             complement(<1..428)
                     /locus_tag="PKV23_01710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HQX84178.1"
                     /translation="MTNLITKIAAAAVFAGLLATAAQAQSKKPFEGVVLVAAVPMDEI
                     QPKALREYATPKFKRGQVVKYATVMAPGSIVVHSKQNVLFYVLDNGKAVKYRVATARK
                     GFEWSGTNKVSFKTEWPDWRPPAEMRARHPELPTFMEGGI"
     gene            634..2652
                     /locus_tag="PKV23_01715"
     CDS             634..2652
                     /locus_tag="PKV23_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013891604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HQX84179.1"
                     /translation="MTIKTVKKFPHKIKEVENFWIPLSDGVKLAARMWIPLDAEKHPV
                     PAVLEYLPYRKRDGTIVRDALTHPYLAGHGYAAIRVDMRGNGDSDGVMLDEYARQEQD
                     DALEVIAWIARQKWSSGKVGMFGISWGGFNALQVAARKPPALKAIVTLCSTDDRYEDD
                     IHYKGGCLLNENLGWAATMLAYSSRPPDPKIVGAKWRKMWLERLKAEPFLLIPWLQHP
                     HRDAYWKHGSVCENFSAIDTPALIVGGWNDAYSNAIPRLMKGLRTTRKAIIGPWSHKY
                     PHFAVPEPRIGFLQEMLRWWDQWLKNTETGVSRDPDYRVYVMDADKPGTSKAHLPGRW
                     IGDSYWGFGNTETKKWFLTGNGISGAPGTEKPLTISSRQTTGGDGGEYCIIWLGPEFP
                     GDQKNDDAQSITFDSPALQTDMDIVGQPQVEIEFSVDKPVASVAVRLNDVWPSGEVSR
                     ITYHLQNLCMRDSRETPTALVPGKRYTMKIKLDDIAWRVPRDHKIRVAVSTSYFPMMW
                     PAPKPVTLTVYAGKSALHLPVRKVVEKETPVLWKPEEAAEPVKLKELKKPWHKREATL
                     DPKTGEWRLAIVDDFGSSEIQPHKLVTYSVGRENYSILPDDPLSARMETHWTEELKRG
                     KWHARTETYGRLTATKTHWLVWGKIDAFEGKKKVFSKEWNEQIERKLQ"
     gene            2872..2975
                     /locus_tag="PKV23_01720"
                     /pseudo
     CDS             2872..2975
                     /locus_tag="PKV23_01720"
                     /inference="COORDINATES: protein motif:HMM:NF016430.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
     gene            3021..3308
                     /locus_tag="PKV23_01725"
     CDS             3021..3308
                     /locus_tag="PKV23_01725"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HQX84180.1"
                     /translation="MRRESAAHFTDLEDFDAPSDDMPGDEAMNRSQEDSHVARALAQI
                     PPEQRELLILSYVDDVPQSMIAERLNLPLGTVKSRMRLAYRRLRKLLEAVN"
     gene            3305..3949
                     /locus_tag="PKV23_01730"
     CDS             3305..3949
                     /locus_tag="PKV23_01730"
                     /inference="COORDINATES: protein motif:HMM:TIGR02451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ChrR family anti-sigma-E factor"
                     /protein_id="HQX84181.1"
                     /translation="MTIVHHLDEATLLSYAAGTLGEALSVAAACHISMCSQCRSAVRQ
                     AEALGGEILDDLESTAVSNARRAATLASLDDAPAVRPVFVKSDMPAPLQRLLQGQGFS
                     QLRWRKKAPGVSVYELSLSKGAKGSLKLLSIASGMTMLEHGHGGEELTLVLQGAFKDK
                     TGRFARGDIADLDEETEHCPVIEKGETCICLYATEAPSHYKTLLGRLLQPFLKI"
     gene            complement(3950..>4766)
                     /locus_tag="PKV23_01735"
     CDS             complement(3950..>4766)
                     /locus_tag="PKV23_01735"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014130346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HQX84182.1"
                     /translation="ENISAHYHLGNNFYKLWLDETMTYSSAVFDRTGNSLEAAQRRKY
                     AKVMEALELKKGDSILEIGSGWGGFAEEAARKKATVKGITLSREQLAFAEERIARAGL
                     ETHASFHFEDYRDTKGSYNGIASIEMIEAVGEPHWPVYFRTLYDRLKPGGIAAIQGIT
                     IAEANFEAYRSGVDFIQRYIFPGGMLLTKTILKDQAAKAGLVLERVETFGQSYATTLR
                     LWHERFEAVWPEVAKLGFDEKFRRLWKLYLAYCEAGFAESVVDVGIYKLRKPA"
BASE COUNT         1003 a         1413 c         1364 g          986 t
ORIGIN      
        1 ttgatgccgc cttccatgaa agttggcagt tccgggtggc gggcgcgcat ttctgctggc
       61 gggcgccaat ccggccattc cgtcttgaag ctcaccttgt tggtgccgga ccattcaaaa
      121 cccttgcggg ctgttgcgac gcggtatttt acagctttgc cattgtcgag aacgtagaac
      181 agaacgttct gcttgctgtg aacgacgatg gagccggggg ccatgacggt cgcatacttt
      241 acgacctggc cgcgcttgaa tttgggcgtg gcatactcgc gaagcgcctt gggctggatt
      301 tcatccatgg gaaccgcggc aaccaggaca acgccttcaa aaggcttctt ggactgggcc
      361 tgggcggcgg tggccagcaa accggcgaat acggcggcgg cggcgatttt tgtgatcagg
      421 ttggtcattt gggcttcccc tcgaaatcag ttgatgctga cggtttacgg gaatgggcct
      481 gaactcatcc tgagcgcggc atttaactgc cgttcatttt cagggctttg cagacgggca
      541 acgggaagct agtctgccgc tccatgccaa atgctcataa aatctggcat tttaggcaaa
      601 aagaccctga aatgccgacc ttccgaggac tccatgacca tcaaaaccgt taagaaattt
      661 ccacacaaaa ttaaggaagt agagaatttc tggatacccc tctccgatgg cgtgaaactc
      721 gcggcccgca tgtggattcc gcttgatgcc gaaaagcacc ccgtgcccgc cgtgcttgaa
      781 tatctcccct accgcaagcg cgacggcacc atcgtccgcg atgcgctcac ccatccctat
      841 ctcgccggcc atggctatgc cgccatccgc gttgacatgc gcggcaacgg cgatagcgac
      901 ggcgtcatgc tcgatgaata tgccaggcag gagcaggacg acgcgctgga agtgatcgcc
      961 tggatcgccc ggcagaaatg gtcaagcgga aaagtcggca tgttcggcat ttcctggggc
     1021 ggcttcaatg cattgcaagt cgccgcgcgg aaacctccgg cgctgaaagc cattgtcacc
     1081 ctatgttcca ccgatgaccg ctatgaggat gatattcatt acaagggcgg ctgcctgctc
     1141 aatgaaaacc tgggttgggc tgcaaccatg ctggcctatt cctcgcgccc gcctgatccc
     1201 aagattgtgg gcgccaaatg gcgcaagatg tggctggagc gcttgaaggc tgaaccattt
     1261 cttctgattc cttggttgca gcacccgcac cgcgatgcat attggaagca tggctccgtc
     1321 tgcgaaaact tctcggcaat cgacacgccc gctctgatcg tcggcggctg gaatgatgcc
     1381 tattctaatg ccatcccgcg cctgatgaag ggcctgcgca ccacgcgcaa ggcaatcatt
     1441 gggccttgga gccataaata tccgcatttc gccgtgcccg aaccccgcat cggattcctg
     1501 caggaaatgc tgcgctggtg ggaccagtgg ctgaagaaca cggaaaccgg ggtttcgcgc
     1561 gaccccgact accgcgttta tgtcatggat gccgacaagc ccggcacctc caaggcccat
     1621 ctgcccggcc gctggatcgg cgattcctat tggggcttcg gcaacacgga gacaaagaaa
     1681 tggttcctga ccgggaatgg catttcagga gcgccaggaa cggaaaagcc gctcacgatt
     1741 tcatcccggc aaacaaccgg cggggatggc ggtgaatact gcatcatctg gcttggcccc
     1801 gaatttcccg gcgaccagaa aaatgatgac gcgcaatcca tcacctttga ctctcctgcg
     1861 ctgcagaccg acatggatat cgtcggccag ccccaggttg aaatcgagtt ttccgtcgat
     1921 aagcctgtcg cgtccgtggc ggttcgattg aatgacgtgt ggccttcagg tgaagtgtcc
     1981 cgcatcacct atcatctgca aaacctctgc atgcgcgaca gccgcgaaac tcctaccgct
     2041 ctcgttcccg gcaagcgcta cacgatgaag atcaagctcg atgacattgc ctggcgcgtg
     2101 cccagggacc acaagataag ggtagccgtt tcaaccagct atttccccat gatgtggccg
     2161 gcccccaaac cggtgacgct taccgtctat gccggcaagt cggcgttgca tctgccggtg
     2221 cgcaaagtgg tcgagaagga aactccagtc ctttggaagc ccgaggaggc cgccgagccg
     2281 gtcaagttga aggaactcaa aaagccctgg cacaagcgcg aagcaacgct tgatcccaaa
     2341 accggggaat ggcgcctggc cattgtcgat gacttcggct ccagcgaaat tcagccgcat
     2401 aaacttgtga cctattcggt gggccgcgag aactactcca tcctgcccga tgatccgctc
     2461 tcggccagaa tggaaaccca ttggaccgag gaactgaagc gtggaaaatg gcatgcccgc
     2521 accgaaacct atggccggct caccgccacc aagacccatt ggctggtgtg ggggaaaatc
     2581 gacgcctttg aaggcaagaa gaaagtcttc agcaaggaat ggaacgagca aattgagcgg
     2641 aagctgcagt gatccgaaag ccactggttt gcgtatatgg aggcgcaggt ccgtcggact
     2701 cgctgtatga ttgccgtgtt gctgcgaatg gctgagaaga cccatattcc tctcccatct
     2761 gcccagcaga ttgattttgc tgctttgctg tttcgcgtgg cccgcgagcg ggaccgcgcc
     2821 gcctttgcgg agatttttga tcacttcgcg ccgcgggtga aaagtttcat gatgcgcaag
     2881 ggcgcttctg ccgaactggc cgaagacctg gtgcaggaaa ccatgatcgc ggtctggtca
     2941 aaggcagccc tctatgtgcc gaaaggggtg ccgcctccac ttgggtattt accattgcgc
     3001 gcaacctgcg catcgaccgt ttgcgccggg agtctgcggc gcattttacc gaccttgagg
     3061 attttgatgc tccatccgac gatatgcccg gcgacgaggc catgaaccgc agccaggaag
     3121 acagccatgt ggccagggcg ctggcgcaaa ttccgccgga acagcgcgag ctactgatcc
     3181 tatcctatgt ggatgacgta ccacagagta tgatcgccga acgattgaac ctcccccttg
     3241 gaaccgtgaa atcccgtatg cgcctggcct accggcgcct gcgcaagctt ttggaggcgg
     3301 tgaattgacc attgttcacc atctggatga agccaccctc ctcagctacg cggcgggaac
     3361 cctcggcgag gccttgagtg tcgcggccgc gtgccacatc agcatgtgtt cgcaatgccg
     3421 cagcgccgtg cgccaggctg aagcattggg tggcgagatc ctcgatgatc tggaaagcac
     3481 cgcagtatca aatgcacgcc gtgccgcaac cctcgccagc cttgatgacg cgcctgccgt
     3541 aaggccagtc ttcgtgaaaa gtgatatgcc agcgccgctc cagcgccttt tgcagggcca
     3601 gggcttcagc cagctgcgct ggcggaaaaa ggcccccggt gtttcggttt atgaattgtc
     3661 actgtccaag ggggcgaagg gctcgttgaa gcttctctcc attgcctcgg gcatgaccat
     3721 gctggaacac ggccatggcg gggaagaact caccctggtc ctgcagggcg ccttcaagga
     3781 caagaccggc aggttcgccc gcggcgatat cgccgatctc gacgaggaga ccgaacactg
     3841 cccggtcatt gaaaaaggcg agacctgcat ctgcctttat gcgacggaag ctccgtcgca
     3901 ctacaagaca cttctcggcc gtctgctgca gccgtttctg aaaatctgat tacgcgggct
     3961 tccgcaattt gtagatcccg acatccacca cggactcggc aaagcccgct tcacaatagg
     4021 ccagatagag cttccacagc cgccggaatt tctcatcgaa gccaagcttg gcgacttccg
     4081 gccacaccgc ttcaaaacgc tcgtgccaca gccgcagcgt ggtggcatag ctttgcccaa
     4141 aggtttcaac ccgctcgagc acaagtcccg ccttggccgc ctggtccttc aggatcgtct
     4201 tggtgagcag catgccgccg ggaaaaatat agcgctggat gaaatccacg cccgagcggt
     4261 aggcctcgaa attggcttcc gcaatcgtga tgccctggat ggcggcgatg ccgcccggtt
     4321 tcagccggtc gtacagggtg cggaaataga ccggccagtg gggctcgccc accgcctcga
     4381 tcatctcgat ggaggcaatg ccgttgtagc tgcccttggt atcgcggtag tcttcgaaat
     4441 gaaaactggc gtgggtctca agccccgccc tggcgatgcg ttcctcggca aaggccagtt
     4501 gttcgcgtga cagggttatg cctttgaccg tggccttctt cctggcggcc tcttccgcga
     4561 acccgcccca gccggaaccg atttccagga tactgtcgcc cttcttcagt tccagggctt
     4621 ccatgacctt ggcgtatttg cgccgctgcg cggcttccag gctgtttccc gtccggtcaa
     4681 aaaccgccga tgaataggtc atggtttcgt caagccagag cttgtagaaa ttattgccga
     4741 gatggtaatg ggccgaaatg ttttcc
//
LOCUS       DAORVN010000054         4760 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_12913_length_4760_cov_3.423592, whole genome shotgun sequence.
ACCESSION   DAORVN010000054 DAORVN010000000
VERSION     DAORVN010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 4760)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4760)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4760
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12913_length_4760_cov_3.423592"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..363)
                     /locus_tag="PKV23_01740"
     CDS             complement(<1..363)
                     /locus_tag="PKV23_01740"
                     /inference="COORDINATES: protein motif:HMM:NF014533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HQX84183.1"
                     /translation="MQLLDQTQRRDWIRLSQSENVGPATFRQLIGRYGSAAAALEALP
                     ELSVKGGLSRPIRIFSLEDAEAVMEQARDLGASFVVAGEAGYPPLLYHIPGAPPLLCV
                     KGNTALAALQCVAIVGARN"
     gene            complement(367..960)
                     /gene="plsY"
                     /locus_tag="PKV23_01745"
     CDS             complement(367..960)
                     /gene="plsY"
                     /locus_tag="PKV23_01745"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HQX84184.1"
                     /translation="MTSFLAVLFGYVCGSIPFGLLLTRAAGLGDVRSIGSGNIGATNV
                     LRTGNKMIAALTLFLDGLKGAVPVLLLTYYGSPQAGMIAGLAAMAGHIFPVWLDFKGG
                     KGVATSLGVLFGLFWPLGLIFIALWLALAFAFRMSSLAGLVTSALTPFWAYLLGCTTE
                     LILSTVIIAVVIWTMHRANIARLMKGEEPKIDLQKRT"
     gene            complement(964..2280)
                     /gene="pyrC"
                     /locus_tag="PKV23_01750"
     CDS             complement(964..2280)
                     /gene="pyrC"
                     /locus_tag="PKV23_01750"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012111757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HQX84185.1"
                     /translation="MMSPAVKQSAQRKAYLNANIVAPAQGLNGKGGILVEDGWILAVG
                     PKVTKDSIGAGTANFDCVGATLIPGLIDMRVFTGEPGTEYRETLATASEAAAAGGVTT
                     MIVMPNTQPVIDDAAIVDFILRRARDTALVRIAPMAAITKGLAGELMSEIGSLKEAGA
                     VAVTDGTHAVSNANLFRRALTYAKDFDMLVVQHVEEASLSKGVMNSSETASRLGLAGN
                     PAMSEVIMLERDIRLVEMTGARYHASQISSSTSLDVIRRAKARGLPVTCGVSIHHLTL
                     NENDIGAYRTFFKLSPPLRTEDDRRALVAGVADGTIDVIVSSHDPQSADTKRLPFAEA
                     AFGAIGLETMLSAAVGLYHNENMPLGRLIEAMSSKPADILGLETGRLAPGAPADFALL
                     DLTMSWTVDETKLRSRSKNSPQEDRTLEGRVIETVVAGRTVYAYAR"
     gene            complement(2277..3239)
                     /locus_tag="PKV23_01755"
     CDS             complement(2277..3239)
                     /locus_tag="PKV23_01755"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012562942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HQX84186.1"
                     /translation="MTSPSERPRLLKGQHLLAVEDISPLEITALLDLAQTYVNEGNGP
                     RQRTPILQGQTQVNLFFEASTRTQSSFEIAGKRLGADVMNMAISSSALSKGETVLDTA
                     MTINAMHPNFLVVRHQSSGAVELLAQKVSCSVINAGDGQHEHPTQALLDALTIRRRKN
                     SIEGLTVAICGDVLHSRVARSNIHMLNKLGARVRVVAPSTLLPTGIANFGVEVHRNMR
                     DGLDGADVVMMLRLQLERMSGAFVPSQREYYRFYGLNEEKLRYAKPDALVMHPGPMNR
                     GIEIDSNVADGAQSVIREQVEMGVAVRMAVLDALSRNWPSGAKS"
     gene            complement(3304..4230)
                     /locus_tag="PKV23_01760"
     CDS             complement(3304..4230)
                     /locus_tag="PKV23_01760"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HQX84187.1"
                     /translation="MTQSFIALLPVFIVILVGYGLRRSELIAEDHWTAVDHVCYYVLF
                     PAIIFKEIAAADFSNVPVWSMALAMILGITSMFALLLALRRPLSSAMDLNGPQFSSLF
                     QGATRWHTFIALAIIPIYFGQPALALGGLSAAAMTPLLNIVNVGVISVYGSGVKMNGR
                     AMALSILKNPFVLSSVGGVVWQLLQLPMPVMGVQVLDMIGKGALGLALLAVGAGLRID
                     EALSTKGPVLLATFLKLLVMPLFMAVWLWVLGVSGEAAAVAILCGAVPTGSGAYVLAK
                     HMGGDAPMIASILTLQVICAAVTIPLVLSLLG"
     gene            complement(4227..4697)
                     /gene="ruvX"
                     /locus_tag="PKV23_01765"
     CDS             complement(4227..4697)
                     /gene="ruvX"
                     /locus_tag="PKV23_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013420037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HQX84188.1"
                     /translation="MSTHLLSLQSALENLPQTSRLMGLDLGTKTIGIATSDRSRQIAT
                     PITTITRTKFTKDATELLAIATKENVGVLVLGLPINMDGTEGPRAQATRAFARNLAKL
                     TPLAIVFWDERLSTAAVERMLIDADASRATRDKVVDKLAAAWILQAALDALRQS"
BASE COUNT         1024 a         1434 c         1418 g          884 t
ORIGIN      
        1 attgcgcgct ccgacgatgg caacacattg aagcgccgcc agagcagtat tgcccttcac
       61 gcagagcagt ggcggcgcgc ccgggatgtg atagagcagg ggcggatagc ctgcctctcc
      121 cgcgaccacg aacgatgctc ccaagtcacg cgcctgttcc atgacggctt ccgcgtcttc
      181 tagcgaaaaa attcggatgg gacgggacaa gccgcctttt accgagagct cgggcaaagc
      241 ctccaaggcc gcggcagcac tcccatagcg gccgatcagc tgccggaatg tcgcgggccc
      301 gacgttttca ctctggctga gccggatcca atcgcgtcgc tgcgtctggt ccagcagctg
      361 catggctcag gttctttttt gaagatcgat cttgggctct tcgcccttca tcaatcttgc
      421 gatattggcg cggtgcatag tccagatgac aactgctatg atgaccgtcg aaagtattag
      481 ttcggtggtg catccaagaa gataagccca gaacggcgtg agcgccgaag taacaagtcc
      541 ggctagtgat gacatgcgga aggcgaaagc aagcgcaagc cagagggcga taaatatgag
      601 tccaagcggc cagaataagc cgaacagcac gccaaggctt gtggccaccc ccttgccacc
      661 cttgaaatca agccagaccg gaaaaatatg cccggccatg gccgcaagcc ccgcgatcat
      721 gcctgcctgc gggctgccat agtaagtgag cagcaacacc ggcaccgcgc ctttgagccc
      781 gtcaaggaac agcgtgagcg ccgcgatcat cttgttgccc gtgcgcagaa cattggtggc
      841 cccgatgttt ccagagccga tgcttcgcac gtctccaagt cccgcggccc gggtgagcag
      901 aaggccgaag ggaatgctgc cacagacata accgaagaga acggcaagaa acgaggtcat
      961 gattcagcga gcgtatgcgt aaaccgtgcg ccctgcaacg accgtttcga tgacccggcc
     1021 ttcgagggta cggtcttcct gcggagagtt cttggagcgt gagcgcagct tggtttcgtc
     1081 aactgtccaa ctcatggtga ggtcaaggag tgcgaagtca gcgggtgccc ccggcgccaa
     1141 gcgtcctgtt tccaacccca gaatgtcggc gggctttgac gacatcgctt caatcagtcg
     1201 ccctaggggc atgttttcat tatggtaaag tccgacggcg gccgacagca tggtttccag
     1261 cccgatggcc ccgaatgccg cttcggcaaa cggcaggcgc tttgtgtcag cgctttgcgg
     1321 atcatgcgag gacacgatca catcgatggt tccgtcggcc actccggcga caagggcccg
     1381 tctgtcatcc tccgtgcgca acggcggcga aagcttgaag aaggttcggt aggcgccaat
     1441 gtcgttttca ttgagcgtca ggtggtggat cgagaccccg catgttaccg gcaggcccct
     1501 ggccttggcc ctgcgaatga catccaggga agtagaactg gaaatttgcg aggcgtggta
     1561 gcgtgcgccc gtcatctcca ccaggcggat atcgcgctcc agcatgatca cttcggacat
     1621 cgccgggttg ccggcgaggc caaggcgcga tgccgtttcc gatgagttca tgacaccctt
     1681 tgacaggctg gcctcttcca catgctggac caccagcatg tcaaaatcct tggcataggt
     1741 aagcgcccgc cggaagagat tggcgttcga aaccgcgtgc gtaccatcgg tgacggcaac
     1801 ggcgccagcc tccttcaggc tgccgatctc gctcatcaat tcaccggcaa ggcccttggt
     1861 gattgcggcc atgggggcaa tccgcaccaa tgccgtgtcg cgggcccggc gcaaaatgaa
     1921 atccacaatc gccgcgtcat cgatcaccgg ctgggtgttg ggcatcacga tcatcgtggt
     1981 gacgccaccc gcggcggccg cttccgatgc ggtggccagc gtctcgcggt attccgtgcc
     2041 gggttcgccg gtgaacaccc gcatgtcgat gaggcctgga atgagggtgg cccccacgca
     2101 atcgaaattt gcggtaccgg cgccaatgga gtccttcgtc accttcggac cgaccgccaa
     2161 tatccagccg tcctcaacca gaatgccacc cttgccattg agaccctgtg ccggggcgac
     2221 aatattggcg ttcagatagg ccttgcgttg cgccgattgc ttcactgcgg gagacatcat
     2281 gattttgctc ccgaaggcca gtttcgcgac agcgcgtcaa gcaccgccat gcggaccgcc
     2341 acgcccattt ccacctgttc ccggatgacc gattgcgctc cgtctgccac gttggagtca
     2401 atttcaatgc cccggttcat cggcccggga tgcatcacca gggcatcagg cttggcatag
     2461 cgcagctttt cttcgttcaa accatagaag cggtaatatt cccgctgcga aggcacgaaa
     2521 gccccgctca tgcgttcaag ctgaaggcgc agcatcatca ccacatccgc cccgtccagc
     2581 ccgtcacgca tattgcggtg tacctccact ccgaaattcg caatgccggt gggcagcagc
     2641 gtggagggag ccacaacgcg aacccgcgcg ccgagtttgt tcagcatgtg aatattggag
     2701 cgcgccacgc ggctgtggag aacatcgccg cagatggcaa ccgtcaaacc ttcaatggaa
     2761 ttcttacggc ggcggatggt gagcgcatcg agaagcgcct gggtcgggtg ctcatgctgg
     2821 ccgtcgcccg cattgatcac cgagcacgaa accttctgcg ccagaagttc aaccgcaccg
     2881 gaggactggt gccgcaccac caggaaattc ggatgcatgg cgttgatcgt catggcggtg
     2941 tccagcaccg tctcgccctt ggagagggcc gaggaggaaa tggccatgtt catgacatcc
     3001 gcccccaggc gctttcctgc aatttcaaat gaactctgcg tgcgggtgga agcttcaaag
     3061 aacaggttga cctgggtctg tccctgcagg atcggagtgc gctgccgggg accgttgccc
     3121 tcgttcacat aggtttgcgc caggtcgaga agcgctgtga tctcaagggg ggaaatatct
     3181 tcgaccgcca gcaggtgctg gcctttgaga agcctgggtc gttcggatgg cgatgtcatt
     3241 aaaagtggct gtgtaggggc gaatctcccg ccccgcaagg gaagatcgct ggcctgtgga
     3301 aaattaaccc aagaggctta acacaagcgg aatcgtgact gctgcacaga tgacctggag
     3361 ggtcagaatg ctggcaatca tgggggcgtc accgcccata tgcttggcaa ggacataggc
     3421 gccggatccc gttggcaccg cgccgcaaag aatggcaacc gccgccgcct caccggaaac
     3481 ccccagaacc caaagccaga ctgccataaa aagcggcata accagcagct tcaggaaggt
     3541 tgccagcagc accggcccct tagtggaaag ggcctcgtcg atgcgcaagc ccgcacctac
     3601 cgccagaagg gccaggccca acgccccctt gccgatcatg tcgagaacct gaacccccat
     3661 cacgggcatg ggcagttgca gcaactgcca gaccacaccc ccaaccgacg acagcacgaa
     3721 cgggttcttc aggatcgaaa gcgccatagc gcgaccattc attttgacgc cggagccgta
     3781 aaccgaaatc acccccacat tcacgatgtt gagaagcggc gtcatggcgg ctgcactcag
     3841 gccgcccaag gcgagggcag gttgtccgaa ataaatggga atgatggcaa gcgcgataaa
     3901 cgtgtgccag cgcgtcgccc cctgaaacag gctggagaat tgcgggccgt tgaggtccat
     3961 ggccgaactc agcggccgcc gcaaagccag cagcagggca aacatgctgg tgatgccaag
     4021 gatcatggca agcgccatgg accacaccgg cacattggaa aaatccgccg ccgcgatttc
     4081 cttgaagatg atggccggaa acaggacgta atagcacacg tgatcaacgg cagtccaatg
     4141 gtcctcggca atcaattcgc tgcgtcgcag gccatagccg acgaggatga ctatgaacac
     4201 cgggagcagg gcgatgaagg attgcgtcat gattgcctca gggcgtccag cgccgcttga
     4261 aggatccagg cggccgccag tttgtccacc accttgtcgc gcgttgcccg cgaggcatcc
     4321 gcgtcgatca gcatgcgctc aaccgcggcg gtggaaagcc gctcatccca gaagacaatc
     4381 gccagcggtg tgagcttggc aaggttgcgc gcaaaggccc gtgtcgcctg cgcccgcggg
     4441 ccctccgtgc catccatgtt gatcggcagg cccaatacca gaacccctac attttccttc
     4501 gtggcaatgg ccagcagctc ggttgcatcc ttggtaaatt tggtgcgtgt tatggtggtg
     4561 atgggggttg caatctgccg gctgcggtcg cttgtcgcaa tgccgatggt cttcgtgccc
     4621 aaatcaagcc ccatcaggcg cgaagtctgg ggcaggtttt ccaaagcgga ctggaggctc
     4681 agaaggtggg tggacatggg cttctgatag ccgatttgtc aaacaggtaa agcagcgtta
     4741 ttgcataacc ccggacgaat
//
LOCUS       DAORVN010000055         4713 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_13158_length_4713_cov_2.627523, whole genome shotgun sequence.
ACCESSION   DAORVN010000055 DAORVN010000000
VERSION     DAORVN010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 4713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4713
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13158_length_4713_cov_2.627523"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1282
                     /locus_tag="PKV23_01770"
     CDS             <1..1282
                     /locus_tag="PKV23_01770"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HQX84189.1"
                     /translation="DSGIAMVFQEQSILPTLTVAENIFLGREEEFLRFGLISKSRMNA
                     AAAEELKKVHLSTHPAMRCADLSFADRQMIEIAKALSLDSRIKTDVTILLDEPTSVLE
                     QKEIELLFRIIGDLKQRASIVFISHRLEEVLEISDRVYVMRDGQIVQELAAKDATVKG
                     LHQHMVGRQLHHEYYREARQATPSAKTVLKCVDLNRDGAFSNISFELHEGEVLGIAGV
                     IGSGREDLARCLAGHTFPDGGNLLVNGTAVHFGAAYDATAKGVGLVPSERKVEGLVAP
                     FSVAENMTLAALPKFVSGGIISFSAEKAVAADWVSRLKIKTPSTATSAGSLSGGNQQK
                     VVLAKWRIAGVRVLILDHPTRGIDVGSKEDVYELVRDMTAEGLAVILLGDTLDEVIGL
                     SNRILVMRDGEITARFEAPPGGKPTQVELVRHMV"
     gene            1284..2234
                     /locus_tag="PKV23_01775"
     CDS             1284..2234
                     /locus_tag="PKV23_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006337166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQX84190.1"
                     /translation="MTQEQRTRLQTWLPLIVLAVLILIVGIYDPRFFSASNFLSVTAD
                     TMTLFLMASGVTLVIMMGGIDLSVQAVASMASCILAAYLQQLGFLAIPLAVLGGALAG
                     FIGGLASTKFRIPSFIATLAVSGVVISAGNWFSQTRSVNIPGDLSATYLDWAIGKALG
                     VPNEIWVGAAFLVIMSAVLGLTPFGRLVRGIGAQERAVIASGINVDRTKIAAFTLSGT
                     MAGVAGVVMAARLGSGSPTLANEFLLPAIACIVVGGTAITGGVGSIWRTFVGALIVQV
                     VRIGMTFMGVSVFAQQIVFGFILVAAVAVTMDRAKVLVIK"
     gene            complement(2227..3246)
                     /locus_tag="PKV23_01780"
     CDS             complement(2227..3246)
                     /locus_tag="PKV23_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003527045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HQX84191.1"
                     /translation="MNKITRRKFGRTGLMVTDMGFGAAPIGNFLKPIPEEVAAAMVEH
                     AWISGMRYFDTAPLYGHGLSELRLGQNLRWKPRDEFVLSSKVGRLLSAAPRASISFAP
                     WVNAAPFTFRFDYSYDGAMRSVEDTLQRTALERIDIAFIHDCDVFTHGAAQQAIYFKQ
                     AMEGAYKALLKMREEGVVKAIGFGVNEWQVCHEALKQGDFDGFLLAGRYTLLEQDSLD
                     EFLPLCEKRNAAVVIGGGLNSGILATGAVPGAKYNYSPAPPHILEKVKKIEAVCTEHK
                     VPLPAAALQFLLAHPAIASHVPGTRTVEQMEQNIAWISHPIPKDFWQDLKHKGLLRKD
                     APVPT"
     gene            3348..4157
                     /locus_tag="PKV23_01785"
     CDS             3348..4157
                     /locus_tag="PKV23_01785"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HQX84192.1"
                     /translation="MKTLNGIPILGCGTFPLKGEEAYRTVRTAIDLGIRHIDTAQMYG
                     NEADVGRALRDCGVPREDLYVVTKVDPGNLSADRFAASVNRSIDDLGGPPDLLLIHWP
                     PAAEEFDGAVERLVLEMQKGMTRHIGVSNFTPNMMRRAYERAGGKIINNQVEFHPLLD
                     QSALLAEARKLGVVLSAYSPLGRGAALKPDAIQEIARRIGRPPSEVVLRWITQQDVVA
                     MPMTTKRENALSNLASLEFELSPGDMAAISRIGRPEGRLINPGWMAGRWSE"
     gene            complement(4293..>4713)
                     /locus_tag="PKV23_01790"
     CDS             complement(4293..>4713)
                     /locus_tag="PKV23_01790"
                     /inference="COORDINATES: protein motif:HMM:NF018012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HQX84193.1"
                     /translation="RPATCDYLGSLDQGMTYSPHALLLCNELAGLLRNLWRGIPVNEE
                     QLALGLAKEVGPRGNYLAHRHTAVNCRAQVWNSRYFGANIPLSNSAKLDQDLFDRIDA
                     DLREHLENHVPAGLAPHIQVELKMIQQRYERAGCGQG"
BASE COUNT          946 a         1370 c         1389 g         1008 t
ORIGIN      
        1 tgattccggc attgctatgg tcttccagga gcagtcgatc ctgcccaccc tgaccgttgc
       61 ggaaaacatc ttcctcggcc gcgaagagga gttcctgcgc tttggcctca tctccaaatc
      121 gcgcatgaat gcggcagcgg cagaggaatt gaagaaggtc catctcagca cccatcccgc
      181 catgcgctgc gccgatctct catttgccga caggcagatg atcgagatcg ccaaggcgct
      241 ttctctcgac agccgcatta agacagatgt tacgatcctc cttgacgaac ccacgtcagt
      301 ccttgagcaa aaggaaatcg aactgctctt caggatcatc ggcgatctga agcagcgggc
      361 ttccatcgta ttcatttcgc accgtctcga ggaggtgctt gaaatctcgg accgtgtcta
      421 tgtcatgcgg gacggccaga ttgtccagga gctggccgca aaggatgcca ccgtcaaggg
      481 gctgcaccag cacatggtgg gacgccagct gcatcacgaa tactaccgtg aggcgcgcca
      541 ggccacgccc tcagcaaaaa ccgtgcttaa atgcgtggat ttgaaccggg atggagcttt
      601 ttccaacatc agctttgaac ttcacgaggg cgaagttctg ggcatagccg gcgtgattgg
      661 ttctggccgt gaagacctgg cacgctgtct cgccgggcat acctttcctg acggcggcaa
      721 cttgctggtc aatggaaccg ccgtgcactt cggagcggcc tatgacgcaa cggccaaggg
      781 cgttggcctt gtaccgagtg aacggaaggt ggagggcctt gtcgccccct tcagtgtggc
      841 ggagaacatg acgcttgccg ccctgccaaa attcgtatcg ggcggcatta tcagtttttc
      901 tgcagaaaag gcggtggcgg ccgactgggt ttcaagactc aagatcaaga cgccgtccac
      961 agcgacttcg gctggaagcc tatcgggcgg caaccagcaa aaagtcgtcc tcgccaagtg
     1021 gcgcatcgcc ggcgtccgtg tcctgatcct ggatcatccc acgcgcggca ttgacgtggg
     1081 ctcaaaggaa gacgtctatg aactggtgcg cgacatgacc gccgaaggac ttgcggtaat
     1141 tctgctgggc gacacgctgg atgaagtgat tggcctgtcc aaccggatcc tcgtcatgcg
     1201 tgacggcgaa attactgcgc gctttgaagc gccccctggc ggaaaaccga cccaggtgga
     1261 actcgtgcgg catatggtgt gatatgacac aggagcaacg aacccgcctg caaacctggc
     1321 ttccgctgat cgttcttgcg gttctcattc tcattgtcgg catttatgac ccccgattct
     1381 tctccgccag caatttcctg tcagtgacgg ccgatacgat gaccctgttc ctcatggcct
     1441 cgggggtaac gctcgtcatc atgatgggcg gcattgacct gtcggtgcag gccgttgcct
     1501 cgatggccag ttgcatcctc gcggcctatc tccagcaatt gggctttctg gcaattccgc
     1561 ttgctgtttt gggtggggcc ctcgcaggat tcattggcgg cctggcgtcg accaagttcc
     1621 gcatcccgtc cttcatcgca acactcgctg tcagtggggt ggtgatcagc gccggcaatt
     1681 ggttttccca gaccaggtcc gtgaatatcc ccggcgactt gagcgccact tatctcgact
     1741 gggccattgg caaagccttg ggcgtaccga atgaaatctg ggtgggggca gccttccttg
     1801 tcataatgag tgccgtgttg gggcttacgc catttggccg ccttgtccgc ggaatcggcg
     1861 cccaggagcg cgcggtgatt gcctccggta tcaatgtgga ccgcaccaag atcgccgcct
     1921 ttaccctgtc aggcaccatg gcgggtgtcg cgggcgtcgt catggcggcc cgcctgggat
     1981 caggctctcc cacactggcc aatgagtttc ttctgccggc aattgcctgc atcgtggtgg
     2041 ggggcacagc cattaccggc ggcgtgggtt ctatctggcg caccttcgtg ggcgccctca
     2101 ttgttcaggt cgtgcggatc ggcatgacgt tcatgggcgt atcggtcttt gcccagcaaa
     2161 tcgtcttcgg cttcattctc gtggctgccg tggcagtgac catggaccgc gccaaggtgc
     2221 tcgtcatcaa gtagggacgg gggcatcctt tctgaggagg cccttgtgct tcaggtcctg
     2281 ccagaaatcc ttcgggatcg gatggctgat ccaggcgatg ttctgttcca tttgttcaac
     2341 ggtccgcgtt cccggcacat gcgaggcgat ggccgggtgt gccagcagga attgaagcgc
     2401 tgccgcaggc agcggcactt tgtgttcggt gcagactgct tcaatcttct tcaccttctc
     2461 caggatgtgg ggcggtgcgg gagaatagtt gtacttggcg ccggggaccg cgcctgtcgc
     2521 aaggattccg ctgttgaggc cgccgcctat cacgaccgcg gcattgcgct tttcgcacag
     2581 tggcaggaac tcatcgagcg agtcctgttc aagcagcgta tagcgccccg caagcaggaa
     2641 accatcgaaa tccccttgct tcagggcttc atggcaaacc tgccattcat tgacgccaaa
     2701 cccgatggcc ttgaccacgc cttcctcgcg catcttgaga agcgccttgt aggctccctc
     2761 catcgcctgc ttgaaataga tcgcctgttg tgctgcgccg tgggtgaaca catcgcaatc
     2821 gtgaatgaag gcgatgtcaa tcctctcaag tgccgtgcgc tgcaaggtgt cctcaactga
     2881 gcgcatcgca ccgtcgtaag aatagtcaaa gcggaacgtg aagggcgccg cgttgaccca
     2941 cggggcgaaa ctgatgcttg cccgcggtgc tgccgacagg agccggccca ccttcgagga
     3001 gaggacaaat tcgtcgcgcg gcttccagcg caggttctgc cccaggcgca gttccgaaag
     3061 cccgtgacca taaagcggag cggtatcaaa gtagcgcatc ccggaaatcc acgcgtgctc
     3121 caccatcgca gcggcgacct cttccggaat gggcttgaga aaattgccga tgggggccgc
     3181 gccaaagccc atgtccgtga ccatgagccc ggtccgccca aatttccgcc gcgtgatctt
     3241 gttcattcgc ttctcccgtt gcatcaggcg gaaggctact ataagtaacc agatggtgca
     3301 atatgtgata ccgtcaacgg gctggaatgg tcaggggagt gcggaagatg aaaacgctga
     3361 acggaatacc cattctcggt tgcggaacct ttccgctgaa gggcgaggaa gcctaccgca
     3421 ccgtccgcac cgcaatcgat cttggcattc gtcatatcga cacggcgcag atgtatggca
     3481 atgaagccga tgtcggccgg gctctccgtg attgcggagt acccagggaa gatctctacg
     3541 ttgtcaccaa ggttgacccg ggcaacctgt ctgcggaccg gtttgccgct tccgtgaacc
     3601 gttcaattga tgatcttggc ggcccgccgg atttgctgct catccactgg cccccggcgg
     3661 cagaggaatt tgacggcgcc gttgagcgtc tggttctgga aatgcaaaag ggaatgaccc
     3721 gccacattgg tgtaagcaac ttcacgccaa acatgatgcg ccgcgcctat gaacgggcag
     3781 gcgggaaaat catcaacaat caggtggagt tccacccctt gcttgaccag agcgcgctgc
     3841 tggccgaggc gcggaaactt ggcgttgtgc tgtcggccta cagtcccctg gggcgggggg
     3901 cagcgctgaa accggatgca attcaggaaa ttgccaggcg catcggaagg ccgccctctg
     3961 aagtcgtgtt gcgctggatc acccagcagg acgtggttgc catgcccatg acaaccaaac
     4021 gcgagaatgc gctgtcaaac ctggcatccc tcgaatttga actttcgccc ggtgacatgg
     4081 cggcaatttc caggattggc aggcccgaag gccggttgat caacccgggc tggatggccg
     4141 gccgctggag cgaatgaagc aggcaaccgg cttggtcaaa ggtgtgcccg tgccaaccca
     4201 gtagcggacc tgctcaatcc cttgactcgg ccaaacttca tcgggactac gatcctctac
     4261 ccaccaaatc tggaaaaatt cggcgcatgg cttcagcctt ggccacagcc ggcgcgttca
     4321 tagcgctgct gaatcatctt tagttcaacc tggatatgag gtgccaatcc agcgggaaca
     4381 tgattttcca ggtgttcacg aagatcggca tcaatgcggt cgaacaaatc ctggtctagc
     4441 tttgcgctat tgctcaatgg aatattcgct ccgaaatagc gcgagttcca gacctgtgcc
     4501 cggcaattca ccgcagtatg gcgatgagcc agataattgc cgcgaggtcc cacttctttt
     4561 gcgaggccaa gcgccagttg ctcttcgtta acaggaattc cacgccagag atttcgaagg
     4621 agcccggcaa gttcattgca cagaagcaag gcgtgggggg agtaggtcat gccttgatcg
     4681 agtgaaccaa gataatcaca ggttgctggc cgg
//
LOCUS       DAORVN010000056         4692 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_13246_length_4692_cov_3.513910, whole genome shotgun sequence.
ACCESSION   DAORVN010000056 DAORVN010000000
VERSION     DAORVN010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 4692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4692
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13246_length_4692_cov_3.513910"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="PKV23_01795"
     CDS             <1..275
                     /locus_tag="PKV23_01795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase type 11"
                     /protein_id="HQX84194.1"
                     /translation="LSGLLKEAQFSAVSWSHALQMPPTKRGFVHSGASAIERIGDLIT
                     SRFSGVIIVEAVKQVYAFSSGKRVRRLIPRFKPALLPSPVRFDGGQ"
     gene            376..1173
                     /locus_tag="PKV23_01800"
     CDS             376..1173
                     /locus_tag="PKV23_01800"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: protein motif:HMM:NF013939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HQX84195.1"
                     /translation="MSEPAGSDVELEDIRREMDAIDDGLVNLLERRFAATEHVRAIKL
                     RNGGLGGSAFRPAREAAILRRLLSRPDSLVPPELLVRLWRNILARSAQNQADITVHIS
                     KKLNASMGLRLRIRDYFGTLPVEEYRDEAQALMQVNASPGDICIVETESLWVEAFVTG
                     KAGQARVIACLPFLREETVPKLLVFGHSPPEPTGDDETLLVSDGKLPRDFSPAPLWQI
                     KLGQHRVSCLPGFLSDHESPLVGLNRSNALLGLKVAGRYPSPFEIKS"
     gene            1170..2273
                     /gene="hisC"
                     /locus_tag="PKV23_01805"
     CDS             1170..2273
                     /gene="hisC"
                     /locus_tag="PKV23_01805"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006936676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HQX84196.1"
                     /translation="MSLKSEASHPLPKAGILDISPYVPGKSAARGAKIYKLSSNESPF
                     GASPQAVAAYSSKSNLLALYPDGSAAELREAIAARYGLNAGSIVCGAGSDELLQLLAH
                     AYLAPGDEAVYSQFGFLVYPIAIASNGAKAVVAPEVNHTTSVDEMLKCVTPRTKMVFL
                     ANPNNPTGTYLPFDEVKRLHAGLPKTTLLVLDAAYAEYVRRNDYESGLELVATSDNVV
                     MTRTFSKIYGLAGLRLGWAYCPAHVADVLNRIRGPFNVSAAAMAAGVAAINDQKFVEA
                     AIAHNEKWLVLLTEQLQSLGLKVTPSVGNFLLIHFAASGKKTAGMADAFLNEHGIILR
                     RMEAYGLPNCLRMTIGDEEANRAVVSALTDFLK"
     gene            2270..3190
                     /locus_tag="PKV23_01810"
     CDS             2270..3190
                     /locus_tag="PKV23_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012110491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate/arogenate dehydrogenase family
                     protein"
                     /protein_id="HQX84197.1"
                     /translation="MSVQFERVALIGIGLIGSSLAHVMRREKIAAHITGYAKSEETRK
                     AAVQLGLVDSIHATAASAVRDADLVILCVPVGACGAIAKEMGAALKPGCIVTDVGSVK
                     AAVVRDVAPHLPAGVHFVPGHPIAGTEQSGPASGFADLFLKRWCILTPLPGSDAVAVM
                     RLEKFWHSCGSYVDQMTPEHHDLVLAITSHLPHLIAYNTVATAADLEEVTDSEVIKYS
                     AGGFRDFTRIAASDPTMWRDVFLNNREAVLEMLGRFSEDLSALQRAIRWGDGETLFNL
                     FTRAREVRRNIIAAGQETAAPDFGRDPKHR"
     gene            complement(3187..4131)
                     /locus_tag="PKV23_01815"
     CDS             complement(3187..4131)
                     /locus_tag="PKV23_01815"
                     /inference="COORDINATES: protein motif:HMM:NF021423.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2125 domain-containing protein"
                     /protein_id="HQX84198.1"
                     /translation="MASKAKRPWYMFYPLTGMILVSLLWCGYWFIAFSGAKELFEAKR
                     LEFAVRGMEFSCAREYWGGFPFRFEFQCGTAKLQFIHGSQTYGVQTSKILAIAQAYNP
                     LHVIVLIDGPTSIDQTAVIHDRALMSVTAGLDQNWDASLETARVNAQGLFTAGQLKLF
                     ARKLNGRLDLAANTKELTVIQPDIPPIIVSSAELVGQTSASLLTQRRLAGHTLDISAL
                     KISQGPVEFAATGTVSLDPQHRLAGKLSSQTNDIDGVMKFIEPIFALNEQDSTTIKKF
                     VSLTGNDPATGTTKADLVAKDGALYWGLLKLADLAPLY"
     gene            4175..>4692
                     /locus_tag="PKV23_01820"
     CDS             4175..>4692
                     /locus_tag="PKV23_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004107692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003839 - gamma-glutamylcyclotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HQX84199.1"
                     /translation="MQQCWIFGYGSLMWRPGFEFESQAHGRIRGFHRSLCVYSLHHRG
                     TEALPGLVLGLDRGGSCVGVAFQIRPSKWEATLAYLREREQVSQVYLEVTQQVELLGG
                     QEKVEAVAFIVDRAHPQYAGKLSHAEILKHVMQGEGKFGHCRDYVMNTLVHLRQMNIH
                     DKTLEAIGAELHL"
BASE COUNT          923 a         1305 c         1448 g         1016 t
ORIGIN      
        1 agctttcggg cctgctcaag gaggcacagt tttcagcggt ttcctggtcc catgccctgc
       61 aaatgccgcc gacaaaaagg ggttttgtcc attcaggggc cagcgccatt gaacggattg
      121 gcgacttgat cacctcccgc ttctctggcg tcatcattgt tgaggcggtg aaacaggttt
      181 atgccttttc ctcaggcaag cgggtcaggc gcctgatccc gcggttcaag ccggctctgc
      241 tgccgtcccc tgtccgcttc gacggcggac aataacgggg aagcctttgc ttttgtgacg
      301 gctcccagta tgaaggtcca atcctgttgg agcggcgcca gtagggccgc cggagtacgt
      361 tagagattga agaccatgag tgaaccagct ggatcagacg ttgagcttga agacatccgc
      421 cgcgaaatgg atgccattga tgacggcctc gtaaacctgc ttgaacggcg ctttgccgcg
      481 accgaacacg tgcgggccat caagttgagg aatggcggtc tgggcggttc ggccttccgg
      541 cctgcacgcg aagcggccat ccttcgccgg cttctgtcac ggcccgatag ccttgtgcca
      601 ccggaacttc tggtgcgtct ttggcgaaac atcctggcgc gctcggccca aaaccaggcg
      661 gacatcacgg ttcatatcag caagaagctc aacgcctcaa tgggtttgcg gctccgcatc
      721 agggattact tcggcaccct tccagtggaa gaataccgcg atgaggccca ggccctgatg
      781 caggtcaacg ccagcccggg agacatctgc attgttgaaa ccgagtctct ctgggtagaa
      841 gccttcgtca ccggcaaggc tggccaggca agggtgattg cgtgcctgcc gttcctgcgg
      901 gaagaaacag ttcccaaact cctggtgttc gggcactcgc cgccggagcc tacaggagat
      961 gatgaaaccc tgctggtcag cgacggcaaa cttccccggg atttttcgcc cgcaccactc
     1021 tggcagatca agctgggcca gcaccgggtg tcttgcctgc cgggattcct gtcagatcat
     1081 gaaagcccgc tcgtaggcct caaccgttcc aatgcgctgc tcggcctgaa ggtggccgga
     1141 cgctatcctt caccattcga gatcaaatca tgagtctgaa gtccgaagcc agccacccgc
     1201 ttcccaaggc gggcattctt gatatttcgc cctatgtgcc gggcaagagc gccgccaggg
     1261 gtgccaaaat atacaagctt tcctccaacg agtcgccgtt tggcgcctcg ccgcaagcgg
     1321 tggcggcgta ttcctccaag agcaacctgc tggcgcttta tcccgacggt tcggccgcgg
     1381 aactgcgtga ggcgattgcg gcgcgatacg gactgaatgc gggatcaatc gtctgcggtg
     1441 ccggctcgga tgaattgctg cagcttttgg cgcatgccta tctggcgccg ggcgacgagg
     1501 cggtttattc gcaatttgga ttcctggtct atcctatagc catcgcctcc aatggggcca
     1561 aggctgtagt tgcgccggaa gtcaatcaca ccacaagtgt tgacgagatg ctgaaatgcg
     1621 tcacaccgcg gaccaagatg gtgtttcttg ccaatcccaa caatcccacg ggcacctacc
     1681 tgcctttcga cgaggtgaag cgcctgcatg cgggactgcc gaaaacaacc ctgctggtgc
     1741 ttgatgcggc ctatgcggaa tatgtcaggc gcaatgatta tgagtctggc ctcgagcttg
     1801 ttgcaacgtc cgacaacgtg gtcatgacgc gcaccttctc gaaaatctac gggcttgcgg
     1861 gcttgcgcct gggctgggcc tactgtccgg cccacgtggc tgacgtgctg aaccggatcc
     1921 gcgggccgtt caatgtcagc gcggcggcga tggcggcggg cgttgcggcg attaacgacc
     1981 agaaatttgt tgaggccgcg attgcgcata atgagaagtg gcttgtcctg ctcacggagc
     2041 aattgcagtc gctgggcctt aaagtcacgc cgagcgtggg aaatttcctg ctcattcatt
     2101 ttgctgccag cggaaagaaa acagctggga tggctgatgc ctttctcaat gagcacggga
     2161 tcattctgcg gcggatggag gcctatggcc tgcccaactg cctgcgcatg accattggcg
     2221 acgaagaagc aaaccgcgcc gttgtgtcgg cccttacgga cttcctgaaa tgagcgtgca
     2281 gtttgaacgc gtggccctta tcggcatcgg cctcatcggc tcgtcacttg cccatgtcat
     2341 gcggcgcgag aagatcgcgg cacatatcac gggctatgca aaaagcgagg agacacggaa
     2401 ggcggcggtg cagcttggtt tggttgacag cattcatgcc accgcggcct cggcagtgag
     2461 agatgcggat ctggttattc tgtgcgtgcc ggtgggcgcc tgcggggcca ttgcgaagga
     2521 aatgggtgcg gcgctgaagc cgggctgcat tgtcacggac gtggggtcgg tcaaagccgc
     2581 cgtggtacgg gatgtggcgc cgcatttgcc ggctggagtt catttcgtgc ccggccatcc
     2641 cattgccggc acggagcaat caggacctgc gtccggcttt gccgacctgt ttctcaagcg
     2701 ctggtgcatc ctcacgccgc ttcccggcag cgatgccgtc gctgtgatgc ggctggaaaa
     2761 attctggcat tcctgcggct cctacgtaga ccaaatgacg cctgagcatc acgaccttgt
     2821 gctggcgatc accagccatc tgccgcatct cattgcctat aacacggtgg ccaccgcggc
     2881 cgaccttgaa gaggtgacgg attcggaagt aatcaagtat tcagccggcg gctttcgcga
     2941 tttcacccgc attgcggcat ccgatcccac catgtggcgc gacgtgttcc tgaataaccg
     3001 cgaagcggtg ctggaaatgt tggggcggtt ttcggaggac ctgtcggcgc tgcagcgggc
     3061 catccggtgg ggcgatggcg aaacgctttt caatcttttc acgcgggccc gcgaagtgcg
     3121 ccggaacatc atcgcggcgg ggcaggaaac cgcggcaccg gatttcggcc gcgatccaaa
     3181 acaccgttaa tagagtggcg cgaggtctgc gagcttgagc aaaccccagt agagggcgcc
     3241 atctttcgca acaaggtctg cctttgtcgt ccctgtcgcg ggatcgttgc cggtcaagct
     3301 gacgaatttt tttattgtgg tactgtcctg ttcattcaag gcaaagatgg gttcaatgaa
     3361 tttcatcacg ccgtcgatgt cgttggtctg gctcgagagc ttgcctgcca accggtgctg
     3421 gggatcgagt gagacagtgc ctgtcgccgc gaactccacg ggcccttgcg aaattttcag
     3481 cgcgcttatg tcgagggtgt gaccggccag acgccgttgc gtgaggagtg aggcgctggt
     3541 ctggccgacg agttcagcac ttgaaacgat gatgggcgga atgtccggct ggattacggt
     3601 gagctctttg gtgttggcgg cgagatccag cctgccgttt aattttctgg cgaatagttt
     3661 cagttggcca gccgtaaaca ggccctgggc gtttactctg gcagtctcca gacttgcatc
     3721 ccagttctga tcaagaccgg ccgtcacgga catcagggcg cggtcatgga taactgctgt
     3781 ttgatcaatt gacgtgggac cgtcgatgag cacaatgaca tggagaggat tataggcttg
     3841 ggcaattgcc aggatttttg atgtctggac gccgtaggtt tggctgccgt ggataaattg
     3901 caactttgcg gtcccgcact ggaattcaaa gcggaaggga aagccgcccc agtattcacg
     3961 ggcgcaggag aactccatgc ctcggactgc gaattccagg cgctttgctt caaagagttc
     4021 cttggcaccc gaaaaggcaa tgaaccagta accacaccag agaaggctga cgaggatcat
     4081 tccggtgagc ggatagaaca tgtaccatgg gcgttttgcc ttggaagcca tatgatctaa
     4141 tgattctcct gaagtgattt gaagcggaaa gagcatgcaa cagtgctgga tatttggcta
     4201 tgggtcgctc atgtggcggc cggggtttga gtttgagtcg caggcgcacg gacgcatccg
     4261 cggctttcac cgcagccttt gcgtttattc gctgcatcac cggggcaccg aggccttgcc
     4321 cggcctggtg cttgggctgg accgcggagg ctcgtgcgtt ggcgtggcgt ttcagatcag
     4381 gccttcgaag tgggaggcaa cgctggctta tctgcgcgag cgcgaacagg tgagccaggt
     4441 ctatctggaa gtgacgcagc aggttgaact tctgggaggc caggagaaag tcgaggcggt
     4501 ggcttttatc gttgaccggg cccatcccca gtatgcgggc aagctctcgc atgcggagat
     4561 tttgaaacat gtcatgcagg gcgaagggaa gttcggccat tgccgcgact atgtcatgaa
     4621 cacgcttgtc cacctccggc agatgaacat ccacgacaag acgctggaag ccatcggcgc
     4681 cgaactgcac ct
//
LOCUS       DAORVN010000057         4690 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_13260_length_4690_cov_3.564401, whole genome shotgun sequence.
ACCESSION   DAORVN010000057 DAORVN010000000
VERSION     DAORVN010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 4690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4690
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13260_length_4690_cov_3.564401"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /locus_tag="PKV23_01825"
     CDS             <1..384
                     /locus_tag="PKV23_01825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HQX84200.1"
                     /translation="GFFDLAPLGNSLAYLRGDEALQRVAVFGLPAALIVWGAMQFKGR
                     KSFWTGQGDASYSLYLTHALFMPVFFLIWKVFPLPTDLMIATSMAASAFFGWRIHLAI
                     EKPVLNALKGERFSRARPHPVASAE"
     gene            418..1290
                     /locus_tag="PKV23_01830"
     CDS             418..1290
                     /locus_tag="PKV23_01830"
                     /inference="COORDINATES: protein motif:HMM:NF017532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HQX84201.1"
                     /translation="MYTKQALHELGIKSNAISEAQKRQLDEQGFFVVENALSKDEVEI
                     LRDEFERIHAAEAEKGGHEVHVEPGARRISNIFNKTTAFDICLNLPPVLAAAHYLLGE
                     IKVHGANLRDPNQGYGHQALHVDVPKRFVDDWWVLNAMLMLDDMTLENGPTRVVPTSQ
                     WWAPINVPYVNQGDWEPEPISAEDQKRIPADLDAPYPGEVYVTAPAGSAIICNSSMWH
                     SGTLKKSSAHRRMLHLTYTRRDLPQQLVQIDHLTQALYDRMSPVHRYLMEIEPPGEGG
                     VLRQKKREHKGWWN"
     gene            1383..2228
                     /locus_tag="PKV23_01835"
     CDS             1383..2228
                     /locus_tag="PKV23_01835"
                     /inference="COORDINATES: protein motif:HMM:NF013918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HQX84202.1"
                     /translation="MTPLTDRQGRFSPLKAATLALALLPAAVIAYWFFTRQLGPLPVK
                     TALHLVGDWTIRLLVITLALTPLQRVLNYPKIALIRRMLGVTTFAYGLTHFLLYIVQS
                     KFDLVFVVTEIVLRFYLTIGFVALIGLSLLAATSFDSAIRKLGKTWKTIHRLVYGIAV
                     LGLLHYFIQSKIDVSPATLMAGFFILLMIYRSFISRRMALTPLVLAISAGLAGLLTAL
                     TEFAWYGLATGVDPWRIAQANLMVSFGLRPAIIVALVGLVPCGFLILRNAHSNGWFKL
                     RKLTA"
     gene            2297..3574
                     /locus_tag="PKV23_01840"
     CDS             2297..3574
                     /locus_tag="PKV23_01840"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HQX84203.1"
                     /translation="MNIQNLPDKQEIETVLGLGPQAQAKRWAKRGIWLAVLALAIAGS
                     YLWYQSSQTTANAVTYETSPARRDDLTVTVTATGTIQPTTQIDVSSEMSGVVRSVNVD
                     NNSLIKKGDVLAELDSERFRGQLKSLQASVESAKAKLADTQATLKASELAYTRQISLQ
                     RRGLAITQDLEAAQAAQQRAIAAVVSAQADIQVAIANLELKQIDIDKSQILSPVDGIV
                     LKRAVEPGQTVASSLQAPVLFTLAEDLKRMQLEADIDEADIGTVKLGQKANFTVDAFT
                     GRNFPALIEAVEFYPETTDGVVTYKAILSVNNSDLALRPGMTATAQVTVQEIPNALLV
                     PNAALRYSPPVAAPQQSFSLSRLFLPRMPRNQKSARTETIAGERTIWVLKNNVPEAVQ
                     IATGASDGKMTEVVKGDIAPDDQLVIAAKQTGK"
     gene            3571..4272
                     /locus_tag="PKV23_01845"
     CDS             3571..4272
                     /locus_tag="PKV23_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019858965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQX84204.1"
                     /translation="MTVAALLALENVSKTYGEGGGMVRALDGVDLTINGGEFVAIMGP
                     SGSGKSTAMNILGCLDSPTKGRFLFNDVDVTKLTRDGRALLRRNFLGFVFQGYNLLSR
                     TTAIENVELPLIYRGTAKRERREQAIGALHLVGLEGRENHTPAELSGGQQQRVAIARA
                     IVTNPLVLLADEPTGNLDTARSNEIMELLSGLNKSRGLTIAMVTHEPDMAAFAKRTIR
                     FVDGKIESDTLQKAA"
     gene            4272..>4690
                     /locus_tag="PKV23_01850"
     CDS             4272..>4690
                     /locus_tag="PKV23_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019171565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQX84205.1"
                     /translation="MLWEAIKLANQSVRRNALRSILTLLGIVIGVGAVIAMVTIGNGT
                     TQKVTADLSKLGSNMLIARPERETFGPGGSGEFRSFTERDVSALRDNLTQVRALAPSS
                     TKSVRIVYGADNVDSTVTGTDSEFFVAQDWTFESGRP"
BASE COUNT          955 a         1439 c         1370 g          926 t
ORIGIN      
        1 ggattcttcg acctcgcgcc cttgggcaat tcgctcgcgt atttacgcgg cgacgaggcc
       61 ttgcagcgcg tggccgtctt tggcctgccc gcggccctca tcgtctgggg cgccatgcag
      121 ttcaagggcc gcaaaagttt ctggaccggc cagggcgatg cctcctactc gctttatctc
      181 acccacgcgc ttttcatgcc ggtgttcttc ttgatctgga aagtgttccc cctgcctacc
      241 gatctcatga tcgcgacgag catggcggcc agcgcctttt tcggctggcg catccatctg
      301 gccattgaaa agccggtgct caatgccctc aagggtgaac gcttttcccg tgcccggccg
      361 catcccgttg cgtcagcgga atagtgtgtt agcctcccgc atcgggagga gcatgccatg
      421 tacacgaagc aggccctgca tgaactggga atcaagagca acgccatcag cgaagcgcag
      481 aagcgccagc tcgacgagca gggcttcttc gtcgtcgaga acgccctgtc aaaagacgag
      541 gttgaaatcc tgcgcgacga attcgagcgc atccatgcgg ccgaggccga gaagggcggg
      601 cacgaggttc atgtggagcc gggggcacgg cgcatttcca acattttcaa caagaccacg
      661 gctttcgata tttgcctgaa cctgccgccg gtgctggccg cggcgcatta tctgctgggc
      721 gaaatcaagg ttcatggcgc caacctgcgc gatcccaacc agggctacgg ccaccaggcg
      781 ctgcatgtgg atgtgccgaa gcgatttgtg gacgactggt gggtgctcaa tgccatgctc
      841 atgctcgatg acatgacgct ggagaatggc cccacccgcg tggtgcccac ttcacagtgg
      901 tgggcgccga tcaatgtgcc ctacgtcaac cagggcgact gggagccgga acccatttcc
      961 gccgaagacc agaagcggat tccggcggac ctcgacgcgc cctatcccgg agaagtttat
     1021 gtgacggcgc cggcgggttc ggccatcatc tgcaattcat cgatgtggca cagcggcacg
     1081 ctgaagaaat cgtccgccca ccggcgcatg ctgcatctca cctatacgcg gcgcgacctg
     1141 ccgcagcagc tggtgcagat cgatcacctg acgcaagcgc tctatgaccg gatgagcccg
     1201 gtccaccgct atctgatgga gatcgaaccg ccgggcgaag gcggggtgct gcggcagaaa
     1261 aagcgtgagc acaagggctg gtggaactga aggtcccacc ctcttcctga tttcaccgct
     1321 tctcccaaaa aggtgaaagc ttgtgtaaag acagtttaag gaagggctgg tagagagccg
     1381 ccatgacccc tcttaccgac aggcagggaa gattttcgcc gctcaaggcc gcaacgctgg
     1441 ccctggcgct gctgcctgcg gccgtcattg cctactggtt ctttacccgc cagctcggcc
     1501 ccctgcccgt caagacggcc ctgcacctgg ttggagactg gaccatccgc ctgctggtga
     1561 tcacgctggc gctcaccccc ctgcagcggg tgctcaacta cccaaaaatt gcactcatcc
     1621 ggcgcatgct gggggtcacc acctttgcct atgggctcac ccatttcctg ctttacatcg
     1681 tgcagtctaa attcgacctg gtcttcgtcg tcacggaaat cgtcctgcgc ttctatctca
     1741 ccattggctt tgtggcgctg atcggcttga gcctgctggc ggccacctcc tttgattccg
     1801 ccatccgcaa gctgggcaag acatggaaga cgatccaccg cctcgtctat gggattgcgg
     1861 tgctggggct tctgcattac ttcattcaat cgaaaatcga cgtgagcccc gccacgctga
     1921 tggccggctt cttcatcctg ctgatgatct accgcagttt catcagccgg cggatggctc
     1981 tcaccccgct cgtcctggcc atcagcgccg gacttgccgg gctgcttacg gcgttaaccg
     2041 agtttgcctg gtatggactg gccacgggag tcgatccctg gcgcatcgcc caggccaatc
     2101 tgatggtttc cttcggcctc cggcccgcca ttattgtcgc gcttgtgggt ctcgttccct
     2161 gcggattcct gatcttgcgc aatgcgcact caaatggctg gtttaagctg cgcaagctta
     2221 cggcataggc tacgccggaa attggctttc ccggcgtcct atccctatat atgcgcagag
     2281 attaaagctg aggtccatga acatccagaa tttgccggac aaacaggaaa tcgagacggt
     2341 attgggcctg gggccacagg cccaggcgaa gcgctgggcg aagcggggaa tttggctggc
     2401 agttctggcc cttgccatcg ccggcagcta tctgtggtac cagagttcgc aaaccaccgc
     2461 caatgccgtg acctatgaga caagcccggc aaggcgcgac gacctgacgg tcaccgtcac
     2521 cgccacgggc accattcagc cgacgaccca aatcgatgtt tcgagcgaga tgtcaggcgt
     2581 cgtgcggtcg gtcaatgtgg acaacaacag cctgatcaaa aaaggcgatg tgctggcgga
     2641 actcgactca gagcgattca gggggcaatt gaaaagcctg caggccagcg ttgagagcgc
     2701 caaggcgaag ctcgccgata cccaagccac cctcaaggcc agcgaacttg cctacacgcg
     2761 ccagatatcc ctgcagagaa gaggcctggc gatcacccag gatctggaag ctgcgcaagc
     2821 cgcccagcag cgggcgatcg ccgcggttgt ttcggcccag gccgacattc aagttgccat
     2881 tgccaatctg gaactgaagc aaattgacat cgacaagtcg caaatcctgt cgccggtcga
     2941 tggcattgtc ctgaagcggg cggttgaacc cggccagact gtggcctctt cgctgcaggc
     3001 cccggtgctt tttacgctgg ccgaggatct gaaacgcatg cagctcgaag ccgacatcga
     3061 cgaggcggac attggaaccg ttaaattggg gcagaaggcg aatttcacag ttgatgcttt
     3121 tactggccgc aattttccgg ccctcatcga agcagtcgaa ttctatccgg aaaccaccga
     3181 tggggtggta acctacaagg ccatactcag cgtcaacaat tccgatctgg cgctccggcc
     3241 aggaatgacg gccacggcgc aagtcaccgt tcaggaaatt cccaatgccc tgcttgttcc
     3301 caacgcggcg ctgcgctatt cgccgccggt tgcagcaccg cagcagagct tcagcctttc
     3361 caggcttttc ctgccgcgca tgccgcgcaa ccaaaagtcg gcacggaccg aaaccattgc
     3421 cggtgagcgc acgatttggg tattgaaaaa caatgttccg gaggcggtgc aaattgccac
     3481 gggagcatct gacggcaaga tgactgaagt cgtcaaaggc gacatcgccc cagacgacca
     3541 gctggtcatc gccgcgaagc aaacgggtaa atgaccgtgg ctgcgttact ggccctggaa
     3601 aacgtgagca agacctatgg cgagggcggc gggatggtgc gggccctcga tggtgttgat
     3661 cttacgatca acgggggcga atttgttgcc atcatgggac cttccggatc cggcaaatcc
     3721 accgccatga acatcctggg ctgcctggat tcccccacca agggacggtt cctgttcaat
     3781 gacgttgacg tgaccaagct gacccgggac gggcgcgcct tgctgcggcg gaatttcctc
     3841 ggcttcgtgt ttcagggcta taatctgctg agccgcacga cggcgattga aaatgtggag
     3901 ctgcccttga tttaccgggg caccgcaaag cgcgaacggc gcgaacaggc tattggtgcc
     3961 ttgcatctcg ttggcctgga ggggcgggaa aaccacacgc cggccgagct ttccggcgga
     4021 cagcagcaac gggttgcgat tgcgcgcgcc atcgtcacga acccactcgt gctgcttgcc
     4081 gacgagccta cgggaaatct cgatacggcc cgcagcaacg aaatcatgga gctgctttcg
     4141 ggactgaaca agtcgcgcgg gcttaccatt gcgatggtga cccatgaacc cgacatggcc
     4201 gctttcgcca agcgcacaat ccgtttcgtt gacggcaaaa tcgaatccga tacgctgcaa
     4261 aaggccgcct gatgctgtgg gaagcgataa aacttgccaa ccagtcggtg cgccgcaatg
     4321 cgctgcgctc catcctcacg ctgctcggca ttgtcatcgg cgttggcgcc gtcattgcca
     4381 tggtcaccat cggcaacggc accacacaga aagtgaccgc tgacctgtcg aagctcggca
     4441 gcaacatgct cattgcacgg ccggaacggg agacatttgg cccgggcggc agcggcgaat
     4501 tccgcagctt cacggagcgc gatgtttcgg cactgcgtga caacctcacc caggtcaggg
     4561 cactggcacc ctcgtcaaca aagtccgtca gaatcgtcta tggcgctgat aacgtggatt
     4621 caaccgtaac cggcacggac tccgagttct tcgtggcgca ggactggacc tttgaatcgg
     4681 gccggccttt
//
LOCUS       DAORVN010000058         4679 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_13320_length_4679_cov_3.524438, whole genome shotgun sequence.
ACCESSION   DAORVN010000058 DAORVN010000000
VERSION     DAORVN010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 4679)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4679)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4679
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13320_length_4679_cov_3.524438"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..556)
                     /locus_tag="PKV23_01855"
     CDS             complement(<1..556)
                     /locus_tag="PKV23_01855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX84206.1"
                     /translation="MHDLSTRALQIRRQKSATSAALLTAVLFAAQFAWTDAIGAPKTT
                     EESAVAVMAPKFQTKVAHDIAIEIVKPGNEGLELSARLSESGGLIERDISWTLRDAQG
                     GIVYDKNTDLAQVGLPPGDYSIEARYGSATFSQNLTLLEANRLMVSFVLEVGGIRVLP
                     RVQGLGLTSAQTQSLVYALSGADKG"
     gene            720..1310
                     /locus_tag="PKV23_01860"
     CDS             720..1310
                     /locus_tag="PKV23_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012113659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HQX84207.1"
                     /translation="MTKLNDLSSPLAFLKTRKSGSVKALGLPGPNPGEISEILDIAVR
                     VPDHGKLTPWRFVLFEGAARADIGQVFAERWKALHPEHGEDSLAFQRGLFLRAPVVIV
                     VISTAGPHVKIPEWEQLMSSAAVCYNIVLAATAMGFDAQWQSDWVAYDPVALAAIGVK
                     ESERVAGIICIGTATAPLEDRPRPDPQAFLTRWVKA"
     gene            1307..2011
                     /locus_tag="PKV23_01865"
     CDS             1307..2011
                     /locus_tag="PKV23_01865"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HQX84208.1"
                     /translation="MTIRGVLFDKDGTLIDVNGTWVPIYRQMLCEQFGISPGEADRMM
                     TRVGYNPNTDGFDANSILAAGTTRQLIDFWWPGLDEAGALEKVRMIDVDYAPLSKSLL
                     KPLMPLVPLLEELKEMALHVGLATNDSHYSAHSHLSHLGILELFDDVIAADTVLTPKP
                     SGDMIRRFAGTVGLKPSEIAMVGDNGHDMEEARNGGAGLAIAVLTGNAGHGDIAHLAD
                     HTLRSIAELPALLRSI"
     gene            2013..2606
                     /locus_tag="PKV23_01870"
     CDS             2013..2606
                     /locus_tag="PKV23_01870"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019962506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HQX84209.1"
                     /translation="MFYDAVRNDHGFEVDPFKALVARPIGWLSTLSADGIANLAPYSF
                     FNIVSERPHYVVFGSTGYKDSLRNIEATGEFAVNVATYSIREAMNMTSSRVAPQVDEF
                     ALAGLSKAPCQFIKPPRVAESPANLECRLFKKIELPDDEGQVANWLVVGRVIGVHIDD
                     KFIENGRVNSGAMQMISRLGYAEYATIDDVWRMRRPD"
     gene            complement(2607..3062)
                     /locus_tag="PKV23_01875"
     CDS             complement(2607..3062)
                     /locus_tag="PKV23_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008837836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HQX84210.1"
                     /translation="MAGLWYEEFEIGKLYRHPITRTVTETDNILFSTLTHNAQPLHID
                     RHFAAGTEWGQPLMNSFFTLGLMVGISVNDLTLGTTIANLGMTEVKFPAPLFQGDTIN
                     VTTEVRSKRESKSRPDAGIVEFDHRAYKQDGALVAQCLRQAFMRKRPVE"
     gene            3373..3738
                     /locus_tag="PKV23_01880"
     CDS             3373..3738
                     /locus_tag="PKV23_01880"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQX84211.1"
                     /translation="MVKCLLIDRDATERKRVSQLLGDLGLSSAERSAADEGLKYCNDN
                     STDLVVMAASPKGMTSSDFIRRMRRTARGKNTVVILYANEPDAEEIGQSIMEGAADFI
                     MQPFDLELLQFKLRQAGVL"
     gene            3837..>4679
                     /locus_tag="PKV23_01885"
     CDS             3837..>4679
                     /locus_tag="PKV23_01885"
                     /inference="COORDINATES: protein motif:HMM:NF021603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2336 domain-containing protein"
                     /protein_id="HQX84212.1"
                     /translation="MRSEPKPGLDTSIFTDVIENGDAMARTRLAVQLAALMCAEDTSK
                     ADCAQVVPVVLKLSVDPVKEVRRTLANGLVGLLTLHADILFSIIADEDDVALPFLAAT
                     PALNHWHMLAVLRVGDVARQQIVVLRSDLSRESLTYAVKSTPLEVCLRLFENHALHLD
                     DRDYHTLYTRFGQAAEMVELLLGRANLPLDIRILQAKRASNRMQQLMAERGWMADNDA
                     SELVADAEETAILRILVEASNEELARAIPFLVSKKMLTPSIILRAAAIGEMKMVQWSL
                     AHLSG"
BASE COUNT          955 a         1289 c         1444 g          991 t
ORIGIN      
        1 ggcccttgtc ggcaccggag agggcataga ccagggattg ggtctgggcc gaggtgagcc
       61 cgaggccctg cacacggggc aaaacccgga tgccgccaac ttccagcaca aagctgacca
      121 tcaggcggtt ggcttcgagc aaggtgaggt tttgcgaaaa ggtggcggag ccgtagcgcg
      181 cttcaatgga atagtctccg ggcggcaggc cgacctgcgc caagtctgtg ttcttgtcgt
      241 aaacgatacc gccctgggca tcgcgcagcg tccatgaaat gtcgcgctct atcaggccac
      301 ccgattctga gagtcttgcc gaaagttcca ggccttcgtt gccgggcttg acgatctcta
      361 tggcgatgtc atgggcgact ttggtctgga attttggcgc catcaccgcc acggcgcttt
      421 cttcggtcgt tttgggagcg cctatggcat cggtccaggc aaactgggct gcaaacagaa
      481 ccgccgtcag aagagctgcg gatgtcgcgc ttttctgacg gcggatttgc agtgcacggg
      541 tgctgaggtc gtgcatgctg tcgtcccctg tttcgttttc ccccagcgtt ccggccggtg
      601 agtgaatcaa agcacggccc tgtcgcttcc ggcaacggga tacttaattt ttagttaatg
      661 aagcacggct aattgaaatc attgcgtttt tgatgttgag ccgccacatt gctggaatca
      721 tgacaaaact gaacgatctg agctcgccgc tcgcctttct caaaaccaga aaatccggct
      781 cagtcaaagc cttaggtctt cctggaccaa atcccgggga aatctcggag attctcgata
      841 ttgcggtgcg ggtgccggat catggcaagc tcacaccctg gcgctttgtg ctgttcgaag
      901 gggcggcgcg ggcggacatc ggccaagtct ttgctgaacg gtggaaagcg ctgcatccgg
      961 agcatggcga agacagcctc gcctttcagc gcggcctgtt cctgcgcgcg ccggtggtga
     1021 ttgtggtgat ttcgacggcg gggccgcacg tcaagatacc ggaatgggag cagctcatgt
     1081 cctcggccgc ggtgtgttac aacatagtgc tggcggcaac agcgatgggt tttgacgccc
     1141 aatggcaatc agactgggtg gcctatgacc ctgtggcact ggcggccata ggggtgaagg
     1201 agagcgaacg ggtggctggc atcatctgca ttggaacggc gaccgcgccg ctcgaagacc
     1261 ggccgcggcc cgacccgcag gcgtttctca cccgctgggt gaaggcatga caatccgcgg
     1321 cgtactcttt gacaaggacg gcacgctgat cgacgtgaac ggcacatggg ttccgatcta
     1381 ccgccagatg ctgtgcgaac agttcggcat cagccccggc gaggccgacc ggatgatgac
     1441 gcgggtggga tataatccga atacggatgg atttgacgca aattcgatcc tggcggcggg
     1501 cacgacgcgc cagctcatcg atttctggtg gccgggcctt gacgaggcgg gagctctgga
     1561 aaaggtgcgg atgatcgatg tcgactacgc gcccctgtca aaatcccttc tcaagcccct
     1621 gatgcccctg gtgccgctgc ttgaggaact caaggagatg gcgctgcatg tggggctggc
     1681 gaccaatgac agccactatt ccgcccatag tcatctcagc catctcggca tactggaact
     1741 attcgatgat gtgattgccg ccgatacggt gctcactcca aagccatcag gcgacatgat
     1801 ccgccggttt gccggaacgg tgggcttgaa gccatcggag attgccatgg tgggggataa
     1861 tggccatgac atggaggaag cgcgaaatgg cggggcgggc ctggccattg cggttctcac
     1921 tggcaatgcc gggcacggcg acatagccca tcttgccgac catacgctgc gcagcattgc
     1981 cgagctgccg gcgcttctga ggtccatctg agatgttcta tgacgctgtc cgcaatgacc
     2041 acggcttcga agttgatccc ttcaaggcgc tggtggcgcg gcccatcggc tggctttcaa
     2101 cgctttcggc cgacggcatc gccaatctcg ctccctacag tttcttcaat attgtttccg
     2161 agcgtccgca ctatgtggtc ttcggatcga ccggctacaa ggacagcctg cgaaacatcg
     2221 aagcgacggg tgaattcgcg gtgaatgttg ccacctattc catccgcgaa gcaatgaaca
     2281 tgacgtcatc gcgggtagca ccgcaggtgg atgaattcgc ccttgccgga ttgagcaagg
     2341 cgccctgcca gttcatcaag ccgccacggg tggccgagag ccccgccaat ctggaatgcc
     2401 ggctgttcaa gaaaatcgaa ctgccggatg acgaggggca ggttgccaac tggctggtag
     2461 tgggccgcgt catcggggtt cacatcgacg acaagttcat tgaaaatggc cgggtgaaca
     2521 gcggcgccat gcagatgatc tcgcgcctgg gctatgcgga atatgccacc attgatgatg
     2581 tctggcggat gcgccggccg gattgatcat tcaacaggcc gtttgcgcat gaaggcctgg
     2641 cggaggcact gcgccacgag tgcgccatcc tgcttataag cgcggtggtc aaactccacg
     2701 atgccggcgt cagggcgtga tttgctttcc cgctttgacc tgacttcggt tgtcacattg
     2761 attgtgtcgc cctggaaaag gggggccggg aattttactt ccgtcatgcc gagattggcg
     2821 atggtggttc ccagggtgag gtcattcacc gaaatgccga ccatgaggcc aagggtgaag
     2881 aaggaattca tcagcggctg gccccattca gttccagcgg cgaaatgccg gtcaatatgc
     2941 aggggctggg cgttgtgagt gagggtggag aacaggatat tatcggtctc ggtcaccgtc
     3001 cgggtaatag gatggcgata aagcttgcca atctcaaatt cctcatacca aaggcccgcc
     3061 atatttttct cccagggcat cgtttcacag ggcaggggcc aaggccagtg gaacttccac
     3121 aaccctgcct tgcttgggga tagcgtctga gtcggcccac gagtcctgaa cggaggctga
     3181 acgccgcaat atagccacac ttgtaagacc tgttgacact agtgatttgt gtttcattgt
     3241 cagtggctta aggtctgtgg aaaagctgtg gaaaaggtgt ttaacgtccc gttaaccaag
     3301 tggggctcag gagtcggcag gcgggaattg tcaggggcag cgctcccgtc gaaacgacaa
     3361 ggaagactcg caatggttaa gtgtttgttg attgacaggg acgccacgga gcgcaagcgc
     3421 gtttcgcaat tgctgggcga tctcggcctc agttccgcgg aacgttccgc cgccgacgaa
     3481 ggcctcaagt actgcaatga caattcgacc gatctggtgg tgatggcggc gagcccgaag
     3541 ggcatgacgt ccagcgattt catcaggcgg atgcgccgga ccgcacgggg caagaatacc
     3601 gtggtcattc tctatgccaa tgagccggat gccgaggaaa tcggccagtc gatcatggaa
     3661 ggggcggctg acttcatcat gcagcccttc gatcttgaac tcctgcagtt caagctgcgc
     3721 caggcgggcg tgctctgact gggcttaagt cttcctaaag agacatggtg aaccaaaaat
     3781 taaggcttga gccgtagcct gagacaatcc tgttacgaat agattggctc ccgtatatgc
     3841 gtagtgaacc caagcccggc ctcgacacca gcatatttac cgacgttatt gaaaatggcg
     3901 acgcgatggc gcgcaccagg cttgccgttc aactggccgc cctcatgtgc gctgaagaca
     3961 cttccaaggc cgattgcgcg caggttgttc cggtggttct gaagctctcg gtcgatccgg
     4021 tgaaggaagt gcgccgtacc ctggccaatg gattggttgg ccttttgacc ctgcatgccg
     4081 acatcctgtt ttccatcatc gccgatgaag acgatgtggc actgccgttt cttgcagcaa
     4141 ccccggcgct caaccattgg cacatgcttg cggtgttgcg ggtgggcgat gtggcgcgcc
     4201 agcagatcgt cgttctccgc tcagatcttt cccgggaatc actcacctat gcggtgaaat
     4261 ccacaccgct cgaggtctgc ctgcggctgt ttgaaaacca tgcgctgcat ctcgatgacc
     4321 gggattacca cacgctttac acgcgcttcg gccaggcggc tgaaatggtc gagcttttgc
     4381 ttggccgggc gaacctgccg ctggatatcc gcatcctgca ggccaagcgc gcctcgaacc
     4441 gcatgcagca actgatggca gaacgcggct ggatggcgga caacgatgcc agcgagcttg
     4501 tggcggacgc ggaagaaacg gccatcctgc ggatcctggt tgaggcaagc aacgaagaac
     4561 tggcgcgggc cattcccttt ctggtgtcca agaaaatgct gaccccttcg atcatactgc
     4621 gggctgcggc aattggcgaa atgaagatgg tgcaatggtc actggcgcat ctttccggg
//
LOCUS       DAORVN010000059         4670 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_13369_length_4670_cov_3.547779, whole genome shotgun sequence.
ACCESSION   DAORVN010000059 DAORVN010000000
VERSION     DAORVN010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 4670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4670
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13369_length_4670_cov_3.547779"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="PKV23_01890"
     CDS             <1..283
                     /locus_tag="PKV23_01890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HQX84213.1"
                     /translation="AHIRFPAFNSRLTPEVAAEVTQISADTSRIDASSPPFYSVRLMI
                     SATELAKLGDNKLKPGMPAEAFIQTEAQTPMTYFLKPLTDQFAHALREE"
     gene            384..1577
                     /locus_tag="PKV23_01895"
     CDS             384..1577
                     /locus_tag="PKV23_01895"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQX84214.1"
                     /translation="MAASGPAFANAEKTNEIIAEQAYQALAAGDQKTAISAYTQAIES
                     QDLPTEGLANALLNRGLAYQQLGEHQEAIDDYTAALRLDAMSAEMRATALYNRGLSQQ
                     KLPNLSAAVEDYTSALFLDPSFAHAYYGRGNALRESGQFLFALSDYEKATRYHHPNAA
                     NVFYAEALTYESLKRPNEARKSLERAVAANPRFKPAVDKLATLQGSVIAKPTAVAAVG
                     GGQVTVRKPDLPQAVEPSPALLGETESDPILTAAVSVPAAIPAKIVDRVPVVEEKIVA
                     IEVVEDDDAPAAVDPVVEPAVAEVGETSEEETPAAPVLEGWSVQVASATSEDGAWSTW
                     KKMKAKHASLAAAKPVVVRADLGTKGIVYRVRLPGFEDQNAAKKSCTKLKSSGVSCYV
                     SKSNS"
     gene            1697..2383
                     /locus_tag="PKV23_01900"
     CDS             1697..2383
                     /locus_tag="PKV23_01900"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF016362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HQX84215.1"
                     /translation="MTVDYSDLMKPWYERLLAPQPPAPEVPGRHSEAEELSVADAHQR
                     ATHSLRLELLGRILAQSPEFFEHLIIDVMLAMGYGARRRDLARRLGRRGDGGVDGVVE
                     QDELGLDVIYLQAKRYKLGSPVPVADVRDFAGTLEAHHAGKGVFVATSQFTRAGRDFV
                     SLLSRRIVLIDGEKLTGLMIRHNIGVKVLETFQFKRLDGDYFSPASFKSRTAETISAS
                     SQPRRKPSEN"
     gene            complement(2296..2913)
                     /locus_tag="PKV23_01905"
     CDS             complement(2296..2913)
                     /locus_tag="PKV23_01905"
                     /inference="COORDINATES: protein motif:HMM:NF021364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding domain-containing protein"
                     /protein_id="HQX84216.1"
                     /translation="MAVDQNLTLASLTDFLSQDYPRLRRYVGETAFNAMARHCLAATD
                     APGLRVLSMHLPDFLALTKPFSRRPEIADLARLERAFQGALNAPEATVIRSAALLPRH
                     IEFHPSVQRLQVRTNATSIWSALTAGELPPSPASLELPQELLVWRQGLASRFRMLGRE
                     EAVAIDAAARGETMKLDQFSEGFLRGWLEAEIVSAVRDLNDAGEK"
     gene            complement(2997..3200)
                     /locus_tag="PKV23_01910"
     CDS             complement(2997..3200)
                     /locus_tag="PKV23_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019198914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HQX84217.1"
                     /translation="MTTVAKVIELTSSSPKSIEDAIETGIKRADSTLDQIEGAWVQDI
                     KCVVKNGKVSEWRVNLKVTFLLK"
     gene            3303..3719
                     /locus_tag="PKV23_01915"
     CDS             3303..3719
                     /locus_tag="PKV23_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015666295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1489 domain-containing protein"
                     /protein_id="HQX84218.1"
                     /translation="MALHLIKLCVGVSEVSELRQWVRDARKGLDSLDHTTRMFPTRAD
                     EILNGGSLYWVIRGMILCRQPIADLVPVKGKDGISRCRIDFKAKIVPVWPTPRRAFQG
                     WRYLSAEDAPADLKKGATASEMDESMRRELSALGLL"
     gene            complement(3722..4567)
                     /gene="panC"
                     /locus_tag="PKV23_01920"
     CDS             complement(3722..4567)
                     /gene="panC"
                     /locus_tag="PKV23_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006200830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HQX84219.1"
                     /translation="MKPKIVRTIEALRRETAKWRNDDLTFAIVPTMGALHEGHLALVA
                     EGLEHADKVLVTIFVNARQFGANEDLSRYPRDEAGDVAKLAEAGAHLIFAPPPEEVYG
                     EGFATTVVLKGPAKANLEDKFRPHFFDGVATIVAKLFIQSGADFAMFGEKDYQQLLVV
                     TQMARDLDMPVRVIGVPTMRAPDGLALSSRNQYLTKTERGYATAIHKSLMQAAEKIRN
                     GTDPQKAMRAASRSLTTLGFKVDYVTARNAETLAVPQSHSDEPLRLLAAAWIGKTRLI
                     DNIEV"
BASE COUNT          933 a         1325 c         1459 g          953 t
ORIGIN      
        1 tgcccatatc cgctttcccg ccttcaactc ccggctgacg cctgaggttg ccgccgaggt
       61 gacgcagatt tccgccgata ccagccggat cgatgcgagc tcgccaccgt tctactcggt
      121 gcgcctgatg atctcggcca cggagctagc aaagcttggc gacaacaagc tgaagcccgg
      181 catgccggcg gaagccttca tccagacgga agcgcagaca ccgatgacct acttcctgaa
      241 gccgctcacg gaccagttcg cccacgcctt gcgcgaggag tagcccggca acggagtttt
      301 aaccctaacg gcctattact gacgggatga gtgtccgtcg tgtaatcctt gcgtctgtta
      361 gttccgcgtt tctcgctgca tgtgtggctg catccggtcc ggcattcgcc aacgccgaaa
      421 agaccaatga aatcatcgct gaacaggcct atcaggcgct ggcggcggga gaccagaaaa
      481 cagcgataag cgcctatacg caggcgattg aatcccagga tttgccgacg gaagggctgg
      541 cgaatgcctt gctcaatcgc ggccttgctt atcaacagtt gggtgagcac caggaagcca
      601 tcgatgatta caccgcggcg ctgcgccttg atgcgatgag cgccgaaatg cgggcaacgg
      661 ccctgtataa ccggggcctg agccagcaga aattgccgaa cctttcggcc gcggttgaag
      721 attacaccag cgcgctgttc ctcgacccca gttttgccca tgcctattat ggccgcggca
      781 acgcgttgcg tgaaagcgga cagttcctct ttgccctgtc ggattatgaa aaagccacgc
      841 gctatcatca tccgaatgcg gccaacgtgt tctatgcgga agcccttacc tatgagagcc
      901 tgaagcggcc aaacgaggcg cggaagtccc tggagcgggc cgttgccgcc aatccgcggt
      961 tcaagccggc ggttgacaag cttgccacct tgcagggcag tgttattgcc aaacctactg
     1021 cagttgcggc tgtcggaggt ggccaggtca cagttcggaa gccagatttg ccccaggcag
     1081 ttgagccaag tccggctttg ctgggcgaga cggagagcga tccaatcctg accgcggcgg
     1141 tgagcgtgcc ggccgcgata cctgccaaga tcgttgaccg ggtgccggtc gtggaagaaa
     1201 aaatcgttgc aattgaagta gttgaggatg atgacgcgcc ggcggcggtt gaccccgtgg
     1261 tcgagcctgc cgttgccgaa gttggcgaaa cctctgaaga agaaaccccc gctgcaccag
     1321 tcctggaagg ctggagcgtg caggtggctt cagctacgag cgaggacggg gcctggtcga
     1381 cgtggaagaa gatgaaggcc aagcacgcat cgcttgcggc cgcgaaacca gtggtggttc
     1441 gcgccgatct cggcaccaag ggcattgtct accgggtgcg cctgcccggc ttcgaggacc
     1501 agaatgcggc caagaagagc tgcacgaagc tcaagtccag cggcgtttcc tgttatgtga
     1561 gcaagagcaa ctcctgatcc catagttgaa gttccatcac cgctggcgcg cagtatttcg
     1621 tcaaaatgga aaggtgaatc gcacctccac ttaaggcgtt tgctttacgc gggttaggcc
     1681 ccgcggagcc cgtagcgtga cagtcgacta ttccgatctg atgaaaccct ggtatgagcg
     1741 attgctggcg ccgcagccgc cagcaccgga ggtgcctggc aggcatagcg aggcggaaga
     1801 actgagcgtt gccgacgccc atcaacgcgc cacccacagc ctgcgccttg agcttctggg
     1861 gcggattctg gcgcagtctc ccgaattctt cgagcacctc atcatcgatg tcatgctggc
     1921 catggggtat ggcgcaaggc ggcgcgatct ggcgcggcgg ctggggcgca gaggcgatgg
     1981 cggcgtcgac ggggtggtgg agcaggatga actgggactc gacgtcatct atctgcaggc
     2041 caagcgctac aagctgggtt cgccggtgcc ggtcgccgat gtcagggatt ttgccggcac
     2101 ccttgaggcg catcatgcgg gcaagggcgt gtttgtcgcc accagccagt tcaccagggc
     2161 ggggcgcgat tttgtttccc tgctgtcacg gcgcatcgtg ctgattgatg gcgagaagct
     2221 gaccggcctg atgatccgcc acaacatcgg cgtcaaggta ctggagacat ttcagttcaa
     2281 gcggctggat ggcgattatt tttcaccggc atcgttcaag tcgcgcacgg ctgaaacaat
     2341 ctcggcttca agccagccgc gcaggaagcc ttccgaaaat tgatcaagtt tcatcgtttc
     2401 gccgcgcgcg gcggcatcga ttgcaacggc ctcttcgcgt cccagcatcc ggaagcgtga
     2461 tgccaaaccc tggcgccaga ccagcagctc ctgcggcaac tccagacttg cgggagatgg
     2521 gggcaattca ccggccgtca gggcagacca aatgctcgtg gcgttggtgc gcacttgcag
     2581 gcgctggacc gaaggatgaa attctatatg gcgcgggagc agggcggcgc tcctgatgac
     2641 agtggcttcg ggggcgttga gggcgccctg aaatgcccgt tccaggcggg cgagatcggc
     2701 aatctcgggg cgtcgggaaa acggcttcgt gagcgcaagg aaatccggca ggtgcattga
     2761 aagcacgcgc aaacctggcg cgtcagttgc ggcaaggcag tgacgcgcca tggcgttgaa
     2821 tgcagtctcg cccacatatc gcctgagcct tggataatcc tggctgagaa aatccgtgag
     2881 gctggcaaga gtcaggttct gatcgacggc catcatccag tttagatgac gaaatccctc
     2941 ttcacatgcc aatcacgagg cggttcgggc cctcacgtta gcgtgatcgg aagggactat
     3001 ttcaagagga aggtcacctt caggttgacg cgccattcgc tgaccttgcc gttcttgaca
     3061 acgcatttga tgtcctgcac ccatgcgccc tcaatctgat caagcgtcga gtcggcgcgc
     3121 ttgatgccgg tttcgattgc atcttcaatg ctcttgggcg acgacgaggt aagttcgatt
     3181 actttggcaa ctgtggtcat ggcttgtatc ctcatgctcg gaaaacattg gctttgaggt
     3241 tttcctcacg gccatgttag cataggtctt tcacctgtag tccagaggaa ttccactcat
     3301 tcatggcgct tcatctcatc aagttgtgcg tcggcgtttc ggaagtgtcc gaactcaggc
     3361 aatgggtgcg ggatgcgcgc aagggcctgg acagcctcga tcacacaacg cggatgtttc
     3421 ccacgcgcgc agacgagata ttgaatgggg gttcactcta ctgggtgatc cgcggcatga
     3481 tcctgtgccg ccagcccatt gcggatctgg tgccagtcaa aggcaaggac ggcatttcgc
     3541 gctgccgcat cgatttcaag gcgaagattg ttccggtctg gcccacgccg cgccgggcgt
     3601 ttcagggctg gcgctatctc agcgcagaag atgcaccggc agatttgaaa aagggcgcaa
     3661 ccgccagcga gatggatgaa tccatgcggc gggaattgtc ggcgctggga ctgctgtaaa
     3721 tttaaacttc gatattatca atcaaccgtg ttttgccgat ccaggcggcg gccaggaggc
     3781 gcaagggttc gtcgctatgc gattgcggca cggcgagggt ttcggcgttt cgcgcggtca
     3841 cgtagtcgac cttgaatccc agtgtggtga gcgagcgcga ggcggcgcgc atggctttct
     3901 gcggatcggt gccgttgcgg attttttctg cggcctgcat cagggatttg tggatggcag
     3961 tggcataacc gcgctcggtc ttggtgagat actggttgcg cgaggatagc gccaggccat
     4021 cgggggcgcg catggttggc acgccaatga ccctgacggg catgtcgaga tcgcgcgcca
     4081 tttgggtcac aaccagcaat tgctggtaat ccttctcgcc aaacatggcg aagtcggccc
     4141 cgctctggat gaacagcttg gccacgatgg tggccacgcc gtcaaagaag tgcgggcgga
     4201 acttatcctc gagattggcc ttggccgggc ctttcaggac cacggtggtg gcaaaaccct
     4261 cgccatagac ttcctccggc ggcggggcaa aaatcagatg ggcaccagct tcagcaagct
     4321 tggcgacatc gccggcctcg tcgcgcgggt agcggctcaa gtcctcattt gccccgaact
     4381 gcctggcatt cacgaaaatc gtcaccagca ccttgtcggc atgctccagg ccttcggcca
     4441 caagcgccag atggccctcg tggagggcgc ccatggtggg cacaattgcg aaggtcaggt
     4501 cgtcgttccg ccacttcgcc gtttcgcggc gcagcgcttc gatggtgcgg acaatttttg
     4561 gcttcacggt ccatggtcct tggggaatcg gcgctacggg cacacacagg aagggcttta
     4621 tatccacata ttttgccgtt aatggcgaga aaaatgtttg ggaatgtggc
//
LOCUS       DAORVN010000060         4651 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_13452_length_4651_cov_2.572454, whole genome shotgun sequence.
ACCESSION   DAORVN010000060 DAORVN010000000
VERSION     DAORVN010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 4651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4651
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13452_length_4651_cov_2.572454"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..524)
                     /locus_tag="PKV23_01925"
     CDS             complement(<1..524)
                     /locus_tag="PKV23_01925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF937 domain-containing protein"
                     /protein_id="HQX84220.1"
                     /translation="MSLLSKTENGDYFGRVGLACNMSAATAEVSLARLCPAIALRLKE
                     KSANDNNAFEALLDLLDEGDDLTGLTDAEAIADGKAVLADLYGSTPAALAEMKKLAPG
                     MSDAQSENISAIAATSVLAVLAKSYTAPATLAGSTDEAPQGGGLMATIMAALIAGLLQ
                     ALRSKFAPRRRRRR"
     gene            complement(530..1156)
                     /locus_tag="PKV23_01930"
     CDS             complement(530..1156)
                     /locus_tag="PKV23_01930"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HQX84221.1"
                     /translation="MPPRLVIFDMDDVLCHYDLGRRLRALSQISGKTPRDIRAAIWDS
                     GFEDDADSGGYPDADVYLAEFSYRIGHTITRQQWIAARRESMIPSGDVLALAEAIGKV
                     SRLAIYTNNGPIVKSSLDELFPEAAAIFKERYCSYEFGTKKPDPDSFTKLLAQIKIPP
                     SDTWFIDDKRSNVQGAKLAGLKAHHFRSYEALAKDVKSMGFAIAGITP"
     gene            complement(1160..2571)
                     /gene="tldD"
                     /locus_tag="PKV23_01935"
                     /pseudo
     CDS             complement(1160..2571)
                     /gene="tldD"
                     /locus_tag="PKV23_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011580082.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
     gene            complement(2639..3280)
                     /locus_tag="PKV23_01940"
     CDS             complement(2639..3280)
                     /locus_tag="PKV23_01940"
                     /inference="COORDINATES: protein motif:HMM:NF018482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion associated locus B family protein"
                     /protein_id="HQX84222.1"
                     /translation="MRITTLFCTFAVAGLLGLFHPVPALAQEEQQPSPPGTAFGPRVK
                     PGQAAQTGQPANPGAGAPPATETVATHGAWQIQCSAPAATGAQAGKKSCGMIQVARSE
                     KNAKVALSLILQHSKQGEKDVTTMRMLVPIGVYLPTGVALEIDGSAVGRAPFVRCRPQ
                     ICEAFAEVSPESIAKMKKGSAANFIIYEAPGLGIPLKVSLEGFSAALENLNTL"
     gene            3635..4492
                     /gene="coxB"
                     /locus_tag="PKV23_01945"
     CDS             3635..4492
                     /gene="coxB"
                     /locus_tag="PKV23_01945"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012116618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HQX84223.1"
                     /translation="MAGMLVFFASVTAWAAAGQPEPWQLGLQDAATPVAEFIAWLHNF
                     LLVIITVITLFVLGLMLYIMLRFNAKANPVPSKNTHNTLLEVAWTVIPILILVAIAIP
                     SLRLLYLQRDIPVADMTVKVIGNPSWNWTYEYPDLGLNDDGSAKVTFTSYLKPKADAE
                     RDNVPYLLATDVPVVVPVNKTVKLIVTSDPEGIIHAWTIPSFGMKIDAIPGRLNEDWF
                     RATQEGVFYGQCSELCGKDHAFMPIEVHVVSDADFAAWSQKAQTASIEEARDFLVAML
                     KAKGQLAQN"
BASE COUNT          898 a         1417 c         1383 g          953 t
ORIGIN      
        1 ttccgccgcc gccgccgtct tggcgcgaac ttgctccgca aggcttgcag caggcccgcg
       61 atgagcgccg ccatgatcgt tgccatgagt ccgccaccct gcggtgcctc atccgtgctg
      121 cccgcaagcg ttgccggagc cgtataggat ttcgccagca ccgccagaac gctcgtggcc
      181 gcgatggccg aaatgttttc gctttgcgca tcgctcattc cgggagccag ctttttcatt
      241 tcggcgagag ccgcaggggt tgagccatag agatccgcaa gcaccgcctt gccgtctgcg
      301 atggcttcgg cgtccgtgag gccggtgaga tcatcgcctt catccaaaag gtcaagcagc
      361 gcctcgaagg cattgttatc attcgcggac ttttccttga gccgaagcgc tatggcgggg
      421 cacagacgcg ccagcgagac ttccgcggtg gcggcactca tgttgcacgc cagtccgacc
      481 cggccgaaat aatcgccatt ctcggttttt gaaagaaggc tcatcattgt catggggtga
      541 ttcctgcgat tgcaaagccc atgcttttga catccttcgc cagcgcctcg taggagcgga
      601 aatgatgcgc cttgaggccg gcgagtttcg ccccctgcac gttcgaacgc ttgtcgtcga
      661 tgaaccaggt gtcggagggg ggaattttta tctgggccag caattttgtg aagctgtcgg
      721 gatcaggctt cttggtgccg aactcatagg agcagtagcg ctccttgaaa atggcggcgg
      781 cttccgggaa cagctcatcc agcgaagact tcacgatcgg gccgttgttg gtgtagatcg
      841 ccagccgtga aaccttgcct atggcttcgg ccagggccag cacatcgccg gacgggatca
      901 tcgattcacg ccgcgccgca atccactgct ggcgcgtaat ggtgtgtccg atgcggtagc
      961 tgaattcggc gagatagaca tcggcatccg gatagccgcc cgagtcggcg tcatcctcaa
     1021 agcctgaatc ccagatggcg gcccggatgt cgcgcggggt cttgcccgat atctgcgaaa
     1081 gggcgcgaag ccggcgtccg agatcgtaat ggcacagcac gtcatccata tcgaaaatga
     1141 cgaggcgtgg tggcatcgct caagccgccg ttccgccaac cgtgagccgg tcaatgcgca
     1201 gggtaggctg gccgacgccc accggaaccc cctggccctg cttgccgcag gtgccgacgc
     1261 ccgaatcgag cgccatgtca ttgcccacca tggaaatgcg ggtcagcgca tcggcacccg
     1321 agccgataag ggtggcgccc ttcaccggcg tggtgatttt gccgtcctcg atcagatacg
     1381 cctcggtgca ggagaacacg aacttgcccg aggtgatgtc cacctggccg ccgccgaagg
     1441 atttgcataa aggcccttct tgatggattt caggatctcg tcgggatgct tgtcgcccga
     1501 cagcatgtag gtgttggtca tccgcggcat cggcgcgtgg gcataggatt gccgccggcc
     1561 attgcccgta ggctttaccc ccatgagccg ggcattgagc cggtcctgca tgaaacccac
     1621 cagcacgccg tcttcaatca gcgttgtgca gttggaaggc gtgccctcgt cgtcgatgct
     1681 gagcgagccg cggcggtcgg ggatagtgcc atcatccacc acggtaatgc ccttggcggc
     1741 aatgcgctgg cccatgagcc cggcaaaggc cgacgtgccc ttgcggttga aatcaccttc
     1801 cagcccatgg ccgatggcct catgcagcag gatgccgggc cagcccggcc cgagaagcac
     1861 atccgtctcg ccggccggcg ccggtttggc gtccagtgat accaggcttt gccggtaggc
     1921 ttcgcgcgcc gccccttgcc agagctcttc ggtcaggtag gaggcgggat cgccgcgtcc
     1981 gcccagccca tggctgccct ggccctgaat gccattgcgc gccgacacga tcgacacgcc
     2041 aaagcgcacc agcggccgca cgtcgcggta aatgctgccg tcgccccgga tgatttcaac
     2101 cgactgccag tcgctggcaa gcgaaaccga aacctgcgca acctccgggc caagggcgcg
     2161 ggcaaagcca tcaacttttt gcagaaggct gatcttctcg gcaaagccga tagcctcaac
     2221 cgggttgtgg gctgaataca gtttctggtt ggtgcgctcc ggcgacgcgg cgatttgccc
     2281 gccggacatc gccattgatt tgcaaagcgc cgcggcgcgc ttgagggctg ccgccgaaat
     2341 atcggttgag tgggcatagg ccgcggtttc gccggtcacc gcgcgcaggc cgaagccctg
     2401 cgattcgtcg aagctggcgg ccttcagttt tccgtcgtcg aaggagaagc cttgcgtttc
     2461 acgcttttca acaaacagct cgccatcgtc ggagcccgcc agggcttgcg ccacaagatc
     2521 ggtggcctgt gcctggctgg tgtcgctgtt gtcaaaaagt gagagggcca acgcaaatgt
     2581 ccttatccaa aagtgtcata cccagataag caccggccac ctgcgccgcc agtcaaattt
     2641 acagggtatt gagattttcc agcgccgccg agaatccttc cagagacact ttcaatggaa
     2701 tgccaagtcc gggcgcctcg tagatgatga aatttgcggc cgaacccttt ttcattttgg
     2761 caatggactc cggcgatacc tcggcgaagg cttcgcagat ctgcggccgg cagcgcacga
     2821 agggcgcgcg gcccacggct gagccgtcaa tttccagggc aaccccggtg ggcaggtaga
     2881 ccccgatggg caccagcatg cgcattgtcg tcacgtcttt ttcgccctgt ttggaatgct
     2941 gcaggatgag cgagagagca acctttgcat tcttttcgct gcgcgccacc tggatcatgc
     3001 cgcaggactt cttgccggcc tgcgccccag tcgctgccgg agcgctgcat tgaatttgcc
     3061 aggcgccatg ggtggcaacg gtctccgtcg cgggcggcgc gcctgcacca ggattcgctg
     3121 gctgacctgt ttgtgcggcc tggcccggtt tgacccgggg gccaaaggct gttcccggag
     3181 gagagggttg ctgctcctcc tgtgccagcg ctggaaccgg atggaaaagg cccaacaggc
     3241 cggcgactgc aaatgtgcaa aacagggtgg tgattctcac gggaacggta cctttcgtat
     3301 gcgaagcgcg gctattacag ccgagttggg tgcaaaacaa caccgatttg gcctaggggg
     3361 ttaaaagctt gctgctgcat ttcatggata aataaggcgg aatttcgaac tctgccaagc
     3421 ctctccggag gattgggcga agcgcctgat tacaatgacg ttatgccgcg cccgcatgag
     3481 ttgcgggtgg gatacgaatg tggttcacat tcagcccgtc attggagtat tcttgaggtt
     3541 tgccaatgac acggcggggc tggccaacac cagctgaaaa agggaccaaa tgagagggtt
     3601 atcaatgaca tttgtaaaca ggctggcggg aacgatggcg ggaatgcttg tctttttcgc
     3661 ttctgtcacg gcctgggccg ctgccggcca gcccgaaccc tggcaattgg gcctgcagga
     3721 tgccgcaacc ccggttgccg agttcatcgc ctggctgcac aacttcctgt tggtgatcat
     3781 caccgtgatc accctttttg tgctgggcct gatgctctac atcatgctgc gcttcaacgc
     3841 caaggcaaac ccggttccct ccaagaacac ccacaacacc ttgcttgaag tggcctggac
     3901 cgtcattccc attctgattc ttgtcgccat cgccattccg tccctgcgcc tgctctatct
     3961 gcagcgcgac attccggtag ccgacatgac cgtcaaggtc atcggcaatc caagctggaa
     4021 ctggacttat gagtatcccg atctcgggct caatgacgat ggttcggcca aggtgacatt
     4081 cacctcctac ctgaagccca aggcggatgc cgaaagggac aatgtcccct atctgctggc
     4141 aaccgacgtg ccggtggtcg ttcccgtcaa taagacggtg aaactgattg tgacctctga
     4201 tcccgaaggc attattcatg cctggaccat tccctccttc ggcatgaaga tcgacgccat
     4261 tccgggccgc ctgaacgaag actggttcag ggccacccag gaaggcgtgt tttacggcca
     4321 atgctccgaa ctgtgcggca aggaccacgc attcatgccc atcgaagtgc atgttgtatc
     4381 tgacgcggac ttcgccgcct ggtcgcaaaa agcccaaacg gccagcattg aagaagcgcg
     4441 cgattttctc gttgccatgc tgaaggccaa gggccagctg gcgcaaaact gaattcattg
     4501 agaggctaac aacccatgtc ctatgcaacc gccgctcacg acgaccacgc cctgccttcc
     4561 ggctggaggc gttacgtcta tgccaccaac cacaaggaca tcggcacgat gtatctgtgg
     4621 tttgccatta tctccgggct tatcggcggc a
//
LOCUS       DAORVN010000061         4642 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_13498_length_4642_cov_2.943972, whole genome shotgun sequence.
ACCESSION   DAORVN010000061 DAORVN010000000
VERSION     DAORVN010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 4642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4642
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13498_length_4642_cov_2.943972"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            104..2563
                     /locus_tag="PKV23_01950"
     CDS             104..2563
                     /locus_tag="PKV23_01950"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003508578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HQX84224.1"
                     /translation="MGSNLSLPKLQAATVEGLSLEIINKLAFSVGKDASSARGHDWLK
                     AAILVVRDRVIEQWMASIRETYQNQGKRVYYLSMEFLIGRLLRDAASNLGLFDTLQAA
                     LENLGVEADLVEALEPDAALGNGGLGRLAACFMESMASLGIPAFGYGIRYRHGLFRQK
                     IIDGVQIELPEDWLVHGNPWEFERREREFEIGFGGTVEASAQLNGVAHYKWKPSEKIL
                     AVAFDTPICGWKGKRVNTLRLWNARPVDPIKLDQFNQGDHIGAQVDTTQAESITRVLY
                     PSDATPAGQELRLRQEYFFTSASLQDLLRRHLNQYGSLLNLAEKAAIQLNDTHPAIAI
                     AELMRLLIDEHNIAWDKAWEITRATTAYTNHTLLPEALESWPVHLIERLLPRHMQIVY
                     AINAGIIADAHKQGMVDFAFLEAISLIDENNGRRLRMGQLAFAGTHSINGVSALHSGL
                     MKETVFKELNSLYPGRINNKTNGITPRRWLQQVNPGLTHLVREAIGPKFLDNAEELIT
                     LDRFANDVGFLEKYSAIKRANKQKLSQLILQRTGIPTDPSALFDVQIKRIHEYKRQLL
                     AILETIALYNSIRAHPGRVTVPRVKIFAGKAAASYWEAKTIIRLINDVGTVINADPAV
                     RGLLKVVFIPNYNVTLAETIVPAADISEQISTAGMEASGTGNMKLAINGALTVGTLDG
                     ANVEISEHAGLDNVFIFGLTAAEVAEQRKSGPVPRDIIDHSPHLKQVIESIHTGIFSP
                     SEPSRYHGLVANLLDHDYFMVAADFAAYSDALGKADAVWRDSSHAWTKKAVHNTARLG
                     WFSADRTIRDYAREIWSIPVA"
     gene            2624..>4642
                     /gene="glgB"
                     /locus_tag="PKV23_01955"
     CDS             2624..>4642
                     /gene="glgB"
                     /locus_tag="PKV23_01955"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012709394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HQX84225.1"
                     /translation="MAAGVLSKAEVDALVVGQHHDPFAVLGLHKVGRSFVARAFVSGA
                     DTLTAHTLAGEKIGDLAKTHEAGFFEGAVKLKDRQPLRFEAANAGGKWNVIDPYSFGP
                     VLGPLDDYYVAQGTHLRLYDKMGAHVISHEGATGVHFAVWAPNAQRVSVIGDFNDWDG
                     RRHVMRKRMGTGVWEIFLPGLAEDVKYKFEIIGADGVRLPLKADPYAFAAELRPNTAS
                     VVASIENFQWTDGDYLTARAQRDARRSPMSIYEVHLGSWRKKDGNGFLSYDELASDLV
                     NYVRELGFTHIELLPISEHPYDPSWGYQPTGLYAPTSRFGDPEGFARFVNAAHEAGIG
                     IIIDWVPAHFPTDEFGLANFDGTALYEHADPRKGFHPDWNTAIYNFGRTEVQSFLINN
                     ALFWLEKYHIDGLRVDAVASMLYLDYSRKSGEWIPNERGGNENLEAIAFLKRMNEVCY
                     GTHPGIITIAEESTAFPGVSQPSYSGGLGFGFKWNMGFMHDTLQYMSRDPIHRRHHHN
                     DLTFGLLYAFTENFVLPLSHDEVVHGKGSLLSKMGGDTWAKFAGLRAYYAFMWAYPGK
                     KLLFMGQEFAPWDEWSEARSLDWHLLGFEPHKGVQTLVSDLNKLYTSSPAMHGRDCEG
                     EGFQWLIADDYTNSVFAWVRWAGEANPIVCISNLTPVPRVGYGVPLPRA"
BASE COUNT          958 a         1393 c         1388 g          903 t
ORIGIN      
        1 aagcactgtt gcgtggcaac aggaacaagt tatacatttg ctcaaattat gatcaattgc
       61 ccaaacaggg ggacgcgtgc aagctacaaa acaaggtata accatgggct ccaacttatc
      121 cttgccaaaa ctgcaggcag caacagttga ggggctgagc ctcgaaatca tcaacaagct
      181 ggcgttttcg gtaggcaagg atgccagctc ggcgcgcggc catgactggc tgaaggccgc
      241 cattctggtg gtgcgcgacc gggtgatcga acagtggatg gcctcgatcc gcgaaaccta
      301 ccagaaccag ggcaagcggg tttactacct ctcgatggaa ttcctcatcg ggcggctttt
      361 gcgcgatgca gcttcgaacc tcgggctgtt cgacacgctc caggcggcgc tcgagaatct
      421 tggcgtcgag gctgatctgg tcgaggcgct ggaaccggac gcagcactgg gcaatggcgg
      481 attggggcgg cttgcggcct gcttcatgga aagcatggca agccttggca ttccggcgtt
      541 cggctatggc atccgctatc gccacggcct gttccggcag aaaatcatcg acggagtgca
      601 gatcgagttg ccggaagatt ggctggttca cggaaacccg tgggagttcg aacggcgcga
      661 acgggaattt gaaatcggct tcggcggaac ggttgaagcc tcggcccaac tcaacggcgt
      721 ggcccattac aagtggaagc cgtcggaaaa aatcctggcc gtggcgttcg atacgccgat
      781 ctgcggctgg aagggaaagc gcgtcaacac gcttcgcctg tggaatgcac ggccggtgga
      841 ccccatcaag ctcgaccagt tcaaccaggg cgaccatatc ggcgcacagg tggatacgac
      901 acaggccgaa agcatcacgc gggttctcta tccctcggat gccacgccgg cgggccagga
      961 actgcggttg cgccaggagt atttcttcac ctcggcctcg ctgcaggacc tgctccggcg
     1021 ccatctcaat cagtacggca gcttgctcaa tctcgccgag aaagctgcca tccagttgaa
     1081 tgacacgcat ccggcaatcg ctattgccga gctcatgcgc ctgctgatcg acgagcataa
     1141 tattgcctgg gacaaggcct gggaaatcac ccgtgccacc acggcctaca ccaaccacac
     1201 gctcttgccg gaggcgcttg aaagctggcc ggtgcatctt atcgagcgcc tgctgccgcg
     1261 ccacatgcag atcgtttacg ccatcaatgc cggcatcatt gccgatgcgc acaagcaagg
     1321 tatggttgat ttcgccttcc tggaagccat atcactgatc gacgaaaaca atggccggcg
     1381 cctgcgcatg gggcaactgg cctttgctgg cacccacagc atcaatggcg tttccgcgtt
     1441 gcattccggc ctgatgaagg aaaccgtgtt caaggaactg aactcgcttt atcccggccg
     1501 gatcaacaac aagaccaatg gcatcacgcc gcggcgctgg ctgcagcagg tcaatcccgg
     1561 cctcacgcat ctggtgcgcg aggcgatcgg gcccaagttc ctcgacaacg ccgaggaact
     1621 catcaccctg gaccgctttg ccaatgacgt gggcttcctc gaaaaatatt ccgcgatcaa
     1681 gcgggcaaac aagcagaagc tttcccagct tatcctgcag cgcaccggca ttcccacgga
     1741 cccttcggcg ctgtttgacg tgcagatcaa acgcatacac gaatacaagc ggcaattgct
     1801 ggccattctt gaaaccatcg ccctttacaa ttccatccgg gcccatccgg gccgggtcac
     1861 ggtgccgcgg gtgaagatat tcgccggcaa ggcggcggcc agctactggg aagccaagac
     1921 gatcatcagg ctcatcaatg atgtgggcac agtgatcaat gccgacccgg cggtgcgcgg
     1981 attgctgaag gtggtcttca tcccgaatta caacgtcacc ctggcggaaa caatcgtgcc
     2041 ggccgccgac atctccgaac agatctcaac cgcagggatg gaagcctccg gcaccggcaa
     2101 catgaagctg gcgatcaacg gggcgctcac cgtgggcacg ctggacgggg cgaatgtgga
     2161 aatttccgag catgcgggcc tggacaacgt cttcatcttc ggactgaccg ccgccgaggt
     2221 ggcggaacag cgcaagtcgg ggccggttcc gcgcgacatc atcgaccatt cgccgcatct
     2281 gaagcaggtc attgaatcga tccacacagg gatattctcg ccttccgaac ccagccgcta
     2341 ccatgggctg gtggcaaacc ttctggacca tgactacttc atggttgcgg ccgactttgc
     2401 cgcctattcg gatgcccttg gcaaggcgga tgcggtgtgg cgcgacagca gccatgcctg
     2461 gactaaaaag gcggtgcata acacggcccg gctcggctgg ttctctgccg accggacgat
     2521 ccgcgactat gcccgggaga tttggtccat tccggtcgcg tgattggcca ttgccccatt
     2581 ccattctgca ttccatagtg agtacaacga gtgtgagtac ccgatggccg caggtgtcct
     2641 aagcaaggct gaagttgacg ctcttgtcgt tggccagcac catgacccct tcgccgtcct
     2701 gggcctgcac aaggttggcc ggagcttcgt ggcccgggcc tttgtcagcg gggcggacac
     2761 gctcaccgcc catacgctcg ccggcgagaa gatcggcgat ctcgcaaaaa cccatgaggc
     2821 cggattcttt gagggcgcgg ttaagctcaa ggaccgccag cccttgcgtt tcgaggccgc
     2881 caatgccggg ggaaaatgga acgtcattga tccttattcc ttcgggccgg tgctgggacc
     2941 gcttgacgac tactacgtgg cgcagggcac gcatctgcgg ctctacgaca agatgggagc
     3001 ccatgtcatt tcccacgagg gcgccacggg cgtgcatttt gccgtgtggg cgcccaatgc
     3061 gcaacgcgtc tcggtgatcg gtgatttcaa cgactgggac ggccgcaggc atgtgatgcg
     3121 caagcgcatg ggcacgggcg tgtgggaaat cttcctgccc ggacttgccg aggacgtgaa
     3181 atacaagttc gagatcatcg gtgctgacgg cgtccgcctg cccctcaagg ctgatcctta
     3241 cgcctttgcg gcggagctgc ggccgaacac ggcgtcggtt gttgcaagca ttgaaaattt
     3301 ccagtggacg gacggcgact atctcacggc gcgggcccaa cgcgatgcgc ggcgttcgcc
     3361 catgtccatc tacgaggtgc atctgggatc gtggcgcaag aaagacggca acggatttct
     3421 gagctacgac gaactggcca gcgatctcgt gaactatgtg cgcgagctgg gtttcaccca
     3481 tatcgaactg ctgcccatct cggagcaccc ctatgatccc tcctggggct accagcccac
     3541 aggcctttat gcgccaacct cgcgctttgg cgacccggaa ggttttgcgc gcttcgtgaa
     3601 tgcggcccat gaggcgggca tcggcatcat tatcgactgg gttccggcgc atttcccgac
     3661 ggacgaattc ggcctggcaa attttgacgg cacggcgctt tacgagcatg cggacccgcg
     3721 caagggcttt catcccgact ggaacacggc gatctataac ttcggccgca ccgaggtgca
     3781 gagcttcctc atcaacaatg ccctgttctg gctggaaaag tatcacattg acggactccg
     3841 agtggatgcg gtggcctcga tgctctatct cgactattcg cgcaagagcg gcgaatggat
     3901 tcccaatgaa cgcggcggca acgagaatct tgaagcgatc gcattcctga aacggatgaa
     3961 cgaggtgtgc tacgggacgc atcccggcat catcacaata gccgaagagt ccacggcgtt
     4021 tcccggcgtt tcacagccct cctacagcgg cggcctgggc ttcggcttca agtggaacat
     4081 gggcttcatg cacgatacgc tgcaatacat gtcgcgcgat cccattcacc gccggcatca
     4141 ccacaatgac ctgaccttcg gcctgctcta tgccttcacg gaaaatttcg tgctgccgct
     4201 gtcgcatgac gaggtggtgc acggcaaggg atcgctgttg agcaagatgg gcggcgacac
     4261 ctgggccaag tttgcggggc tccgcgccta ttacgccttc atgtgggcct accctggcaa
     4321 gaaattgctg ttcatgggcc aggaattcgc gccctgggat gaatggagcg aagcccggag
     4381 cctggactgg cacctgcttg gcttcgagcc gcacaagggg gttcagacgc tcgtttccga
     4441 cctgaacaag ctctacacat cgagccccgc catgcatggc cgggattgcg agggcgaagg
     4501 cttccagtgg ctgatcgccg acgactatac gaattcggtt ttcgcctggg tgcgctgggc
     4561 gggcgaggcc aacccaatcg tctgcatttc aaacctgacg ccggtgccgc gggttggcta
     4621 tggcgtgccg ctgccgcggg cc
//
LOCUS       DAORVN010000062         4637 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_13517_length_4637_cov_2.975993, whole genome shotgun sequence.
ACCESSION   DAORVN010000062 DAORVN010000000
VERSION     DAORVN010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 4637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4637
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13517_length_4637_cov_2.975993"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..701)
                     /locus_tag="PKV23_01960"
     CDS             complement(<1..701)
                     /locus_tag="PKV23_01960"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018387210.1"
                     /note="catalyzes the interconversion of D-xylose to
                     D-xylulose; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HQX84226.1"
                     /translation="MTKFYSITDPIKYEGPESKNALAYRWYNPKQKVMGKSMAEHLRF
                     AVCYWHTLCWPGTDPFGGETFNRQWHNMADPMAAARMKADMMFETLRLLGIDFFTFHD
                     LDIAPEGKSLKEFNSNVTAIANILEGKMAKSGKKLLWGTANMFSNRRFMAGAATNPDP
                     DIFAYCAAQVKHCLDVTKGLGGENYVMWGGREGYETLINTNIGQELKQAGRFLSLVVE
                     YKHKIGFKGPILIEP"
     gene            complement(698..2158)
                     /gene="xylB"
                     /locus_tag="PKV23_01965"
     CDS             complement(698..2158)
                     /gene="xylB"
                     /locus_tag="PKV23_01965"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018064737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - xylulokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HQX84227.1"
                     /translation="MYLGLDFGTSSVKGVLIDERQKIIGTASSPLKVSRPHDGWSEQK
                     PEDWWKAAHAVVKSLRKAKPKAVAAVDGIGLSGQQHGATLIDKAGHVLRPCILWNDAR
                     SFEECKDILAREPRALAISGNIPLAGFTAPKLVWVKKHEPRIFDAVDKVLLPKDYIRY
                     RMTGEFASDMSDSSGTYWLDVAKRQWSDDLLRATDMRREQMPRLCEGTDATGRLTAAV
                     AKAWGMPKRPVVAGGGGDNAASACGIGAVTDGAAFVSIGTSGVMFVSNDKFSPNAARA
                     VHAFCHAVPGTWHQMGVILSATASLEWLAGVLKTPAPQLTKALGTKLSGPSSALFLPY
                     LSGERTPVGDAQVRGLMMGLGHETDARTMTHAVLDGVAFAFRDSLVALQDAGTNVKRV
                     MAVGGGTKSDLWLRIIATALGVPVDLPAAGDVGGAFGAARLGLMAATGASYRTVCTPP
                     KIARTIQPDAKARAAYEEAYGRYQKIYPAIKEINRS"
     gene            complement(2170..3195)
                     /locus_tag="PKV23_01970"
     CDS             complement(2170..3195)
                     /locus_tag="PKV23_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HQX84228.1"
                     /translation="MARVTIEDVALAAGLSVATVDRVLNGRAAVRPQTAQKVEKAIRQ
                     LNYQPDRLAARLAKGQEYRFCFVLPEGNNDFMIGLGEEVRAMASHLVSERVQIDLRLT
                     DVFDAATLAATLDSIGDIYDGVAVVALDHPRVREAINGLVERGVSVVTLVSDVPSSKR
                     VHYAGIDNSSAGRTAATLMGRFLGGKTGKVGLIAGSLSLRDHIERRFGFEQVLAQEFP
                     QLAILPVLESRDDWQRVEKVTVEMLSAHPDLIGIYNVGAGARGIVAALENTGRQKQVT
                     YIAHELTDHTRRALVDGTIDAIINQNAGHEVRSAVRVLMAKADRTPLIEAMEHIRIDI
                     FVRDNLP"
     gene            complement(3258..4190)
                     /locus_tag="PKV23_01975"
     CDS             complement(3258..4190)
                     /locus_tag="PKV23_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007197240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HQX84229.1"
                     /translation="MFYGEPEGTGFEAIDPRFNECLTGHGRVERLWTGARWAEGPAWF
                     AAGRYLVFSDIPNNRMMRYDETDGSMSVFRSPAHNSNGNTVDRQGRLVTCEHQARRVT
                     RTEHNGSITVIADAFDGKRLNSPNDVVVKSDGSIWFTDPPYGHLTDYEGDRGSSEIGN
                     ANVYRVDPASGKVAAVCTDFDKPNGLAFSPDEKFLYIADTGASHKAGGNKHIRLFEIA
                     ANGQGLGKSKVFADCTAGLFDGFRLDREGRIWTSAGDGVHCYLPDGTLIGKIVIPELV
                     ANVCFGGAKLNRLFICGTTSLYSAYLAVNGISPC"
     gene            complement(4268..>4637)
                     /locus_tag="PKV23_01980"
     CDS             complement(4268..>4637)
                     /locus_tag="PKV23_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007752180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HQX84230.1"
                     /translation="TQMQELAPLKDNKRAFDVMIEASGNEQAILTGLDVLKPRGILVQ
                     LGLGGSMGLPQNLVVSKEVEIRGSFRFHEEFALAVDLINRRRVDLTPLLTKVLPLDQA
                     EKAFQLASDRSRAMKVQIAF"
BASE COUNT          863 a         1458 c         1390 g          926 t
ORIGIN      
        1 ttgggctcga tgaggatagg tcccttgaag ccaatcttgt gcttgtactc gacaacgagc
       61 gagaggaagc gccccgcctg tttcagttcc tgcccgatat tggtgttgat cagcgtttca
      121 tagccctcgc gcccgcccca catcacgtag ttttcaccgc ccagcccctt ggtcacatca
      181 aggcaatgct tcacctgcgc cgcgcaatag gcgaagatat ccggatcagg attggtggca
      241 gcgcccgcca tgaagcgccg gttggaaaac atgttggccg tgccccagag cagcttcttt
      301 cccgacttcg ccattttgcc ttcgaggata ttggcgatgg ccgtcacatt gctgttgaac
      361 tccttcagcg acttgccttc cggcgcgatg tcgagatcgt gaaaggtgaa gaagtcgatg
      421 ccgagcagcc tgagcgtttc aaacatcatg tcggccttca tccgtgccgc cgccatcggg
      481 tccgccatgt tgtgccactg acggttgaag gtttcgccgc cgaaggggtc ggttcccggc
      541 cagcacagcg tgtgccagta gcacaccgca aagcgcaagt gctcggccat gctctttccc
      601 atgaccttct gcttcggatt gtaccagcga taggccaggg catttttgga ctctggcccc
      661 tcatacttga tggggtcggt gatcgaataa aatttcgtca tgagcggttg atctccttga
      721 tcgctggata gattttctgg tagcgcccgt aggcctcttc gtaagccgcc cgcgccttgg
      781 cgtcgggctg gatagttcgg gcaattttgg gcggcgtgca gaccgtccgg taggaggcgc
      841 cggttgccgc cattagtccc aggcgcgccg caccgaaggc cccgcccaca tcgccggccg
      901 ccggaagatc gaccggaacg ccaagcgccg tggcgatgat ccggagccag aggtcggact
      961 ttgttccgcc gcccaccgcc atcacccgct tcacgtttgt gcccgcgtcc tggagtgcca
     1021 caaggctgtc gcggaaggcg aaggccacgc cgtcaagcac cgcatgggtc atggtcctcg
     1081 catcggtctc gtggccaagc cccatcatca ggccgcgcac ctgggcgtcg cccaccgggg
     1141 tgcgctcgcc ggagagatag ggcaggaaca gcgccgaaga tggcccggaa agctttgtgc
     1201 caagggcttt ggtgagctgc ggcgcgggcg ttttcaaaac ccctgccagc cattccaggc
     1261 tcgccgttgc cgaaaggatg acgcccatct ggtgccaggt gccgggaacc gcatggcaga
     1321 atgcatgcac cgcccgggcc gcattggggc tgaatttgtc gttggagacg aacatgacgc
     1381 ccgacgtgcc gatcgatacg aaggccgccc cgtcggtgac cgcccctatg ccgcaggccg
     1441 aagccgcgtt gtcgccgccg ccgcctgcca ccaccgggcg cttcggcatg ccccaggctt
     1501 tggccaccgc ggctgtaagc ctgccggtgg catcggtgcc ctcgcagagg cggggcatct
     1561 gctcccgccg catgtccgtc gcccgcagca ggtcatccga ccattgccgc ttggcgacgt
     1621 cgagccaata ggtccccgac gagtccgaca tgtccgaggc aaactctccc gtcatgcggt
     1681 agcggatata atccttgggc agcagaacct tgtcgaccgc atcaaaaatc ctgggctcat
     1741 gtttcttgac ccagaccagc ttgggcgcgg tgaagcctgc gagcggaatg ttgcccgata
     1801 tcgcaagggc cctaggctcg cgcgccagaa tgtctttgca ctcctcgaat gagcgcgcat
     1861 cattccacag gatgcagggg cgcagcacgt ggcccgcctt gtcaatcagc gtggcgccat
     1921 gctgctggcc ggaaaggccg atgccatcga cggcggcaac ggccttgggc tttgccttgc
     1981 gcagcgattt gaccaccgca tgggccgcct tccaccagtc ttccggcttc tgctcggacc
     2041 agccgtcatg cggccgcgaa accttgaggg gcgaacttgc tgtgccgatg attttctgcc
     2101 gctcgtcaat cagcacgcct ttgacggaag aagttccaaa atcaagaccg agatacatga
     2161 gtttgctttc taaggaagat tgtcgcgcac gaagatatcg atgcggatgt gttccatcgc
     2221 ttcgatgagg ggagtccggt cggccttggc catcagcacg cgcaccgcgc tgcgcacctc
     2281 atggcctgca ttctggttga tgatcgcatc gatcgttccg tctaccaggg cgcggcgggt
     2341 gtgatcggtg agttcatgag cgatataggt gacctgcttc tgccgcccgg tgttttccag
     2401 cgccgcgaca atgccgcgcg cgccagcacc cacattgtag atgccgatga ggtcggggtg
     2461 cgccgacagc atttccactg ttactttttc aacccgctgc cagtcgtcgc gcgactcgag
     2521 gaccggcagg atggcgagct gcgggaactc ctgggccagc acctgctcaa agccgaagcg
     2581 ccgttcgata tggtcgcgca atgaaagcga accggcgatc agcccgacct ttcccgtctt
     2641 gccgccgaga aaacggccca tcagggtggc cgcggtgcgc ccggcactgg aattgtcgat
     2701 ccccgcataa tgcacccgct tcgagctcgg aacatcggac accagcgtca ccaccgaaac
     2761 gccgcgctcc accaggccat tgatggcttc ccgcacccgc gggtggtcca gcgccaccac
     2821 ggcgacgcca tcgtaaatat cgccgatgct gtccagggtt gcggccagcg ttgccgcgtc
     2881 aaatacatcg gtgaggcgca ggtcgatttg cacccgctct gaaaccagat gcgaagccat
     2941 ggcgcgcacc tcttcgccaa gcccgatcat gaagtcgttg ttgccttccg gcaggacgaa
     3001 acagaagcgg tattcctggc ccttcgccag gcgcgccgcc agccggtcgg gttgatagtt
     3061 gagctggcgg atggcttttt ccaccttctg ggcggtttgc ggccgcaccg ccgcgcgccc
     3121 gttcagcacc cggtcaacgg ttgcaacact gaggcctgcc gcaagggcca cgtcttcaat
     3181 ggtcactcta gccatggctc atatggactc cgcgccggaa ttttgtcaat tcctgatagg
     3241 cgtctatcaa ggcttaatta gcagggtgaa atgccgttga cggcgagata ggccgaatac
     3301 agcgaggtgg ttccgcagat gaagaggcgg ttgagcttgg ccccgccgaa acagacatta
     3361 gccaccagtt cgggaatgac aatcttgccg atcagggtgc cgtcgggaag atagcagtga
     3421 accccgtcgc cggcgctggt ccagatgcgg ccctcacggt ccagccggaa gccgtcaaac
     3481 agccccgccg tgcaatcggc aaagactttc gatttgccca ggccctggcc gttcgcggca
     3541 atttcaaaaa gccggatatg cttgttgccg cccgccttgt ggctggcgcc cgtgtcggca
     3601 atgtagagaa atttttcatc gggcgagaaa gcgagcccat tgggcttgtc gaagtcggtg
     3661 cagacggcag ccactttgcc gctggcgggg tcgacccggt aaacattggc gttgccgatt
     3721 tcgctgctgc ccctgtcgcc ctcatagtcg gtgagatggc cgtaaggcgg atcggtgaac
     3781 cagatcgaac cgtccgattt caccaccacg tcattggggg aattgaggcg cttgccgtca
     3841 aatgcgtcgg caatgacggt gatcgaacca ttatgctccg tgcgggtcac ccgccgggcc
     3901 tggtgttcgc aggtcaccag gcgcccctgg cggtcaacag tatttccgtt ggagttgtgg
     3961 gccgggctgc ggaacaccga catcgagcca tcggtttcgt cgtaacgcat catgcggttg
     4021 ttgggaatgt ccgagaacac gagataacgg cccgccgcga accacgccgg cccctccgcc
     4081 cagcgcgccc ccgtccacag ccgctcaacc cgcccatggc cggtgaggca ttcgttgaaa
     4141 cgcgggtcga tggcctcaaa gcccgtgcct tccggctctc cataaaacat ctgcaactcc
     4201 cattggattt tttatttgtc ttacatattt gataacaaac aaacttcgga agtgaaaggg
     4261 cccgcatcta aaaggcgatc tggaccttca tggcgcgcga cctgtcgctg gcaagctgaa
     4321 aggccttctc tgcctggtcc aggggcagga ccttggtgag cagcggcgtg agatcaacac
     4381 ggcgccggtt gatgagatcg accgccaggg caaattcctc gtgaaagcgg aatgaaccgc
     4441 ggatttcgac ttccttggat acaaccaggt tttgcggaag tcccatgctg ccgccgagcc
     4501 cgagctgaac cagaatgccg cgcggcttca gcacatcgag gcccgtcaga atggcctgct
     4561 catttcccga ggcctcgatc atcacatcga atgcgcgctt gttgtccttc agcggcgcga
     4621 gctcctgcat ctgcgtt
//
LOCUS       DAORVN010000063         4562 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_13921_length_4562_cov_3.103617, whole genome shotgun sequence.
ACCESSION   DAORVN010000063 DAORVN010000000
VERSION     DAORVN010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 4562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4562
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13921_length_4562_cov_3.103617"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..862)
                     /locus_tag="PKV23_01985"
     CDS             complement(<1..862)
                     /locus_tag="PKV23_01985"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011663030.1"
                     /note="acts in conjunction with GvcH to form
                     H-protein-S-aminomethyldihydrolipoyllysine from glycine;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine dehydrogenase
                     (aminomethyl-transferring)"
                     /protein_id="HQX84231.1"
                     /translation="MSRASLADLEPGANFIRRHIGPSETETTDMLREVGAKSLEDFIA
                     KVVPKEIRMARPLDLPKAKAERTALSYLRRMADRNEVFTSMIGMGYYGTVTPKVILRN
                     VLENPGWYTAYTPYQAEVSQGRLEALLNFQQVIVDLTGLELANASLLDEATAAAEAMA
                     MAKRLVKTTANAFFVDRDTHPQTIGVIQTRARAFGYEIIIGDPAKDLKPETVFAALLS
                     YPGSSGEIRDYRGVIDKLHGAGAMAIMATDLLALALLTPPGELGADIAVGSAQRFGVP
                     MGYGGPHAGYL"
     gene            complement(874..1245)
                     /gene="gcvH"
                     /locus_tag="PKV23_01990"
     CDS             complement(874..1245)
                     /gene="gcvH"
                     /locus_tag="PKV23_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019963101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HQX84232.1"
                     /translation="MSVKFSKDHEWVSVAGDIATIGITNHAQEQLGDVVFVELPAVGK
                     KVAKGGDAAVVESVKAASEVYAPVTGEVVEVNKELEGDPALVNRGAEGDGWFMKVKIS
                     NPAELNELMDKAAYDKFVASL"
     gene            complement(1257..2384)
                     /gene="gcvT"
                     /locus_tag="PKV23_01995"
     CDS             complement(1257..2384)
                     /gene="gcvT"
                     /locus_tag="PKV23_01995"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018697621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HQX84233.1"
                     /translation="MNETALKRTILYGEHAALGAKLVPFAGYEMPVQYPTGILTEHNW
                     TRTSAGLFDVSHMGQAFLVGPDHATTAAAMEKLVPADIASLKPHQIRYSQFLNPEGGI
                     LDDLMITRSAYAGYEGWLYLVCNASRKDDDFAHIERHLPAHVKLQRGADMALVALQGP
                     KAEAALAEFVPAVRDIPFMHYAAVSIDGIEAHVSRSGYTGEDGFEISIFDKDAVRLWQ
                     LLLANPDVKPIGLGARDSLRLEAGLCLYGHDIDESTSPVEGDLLWSIQKRRRNEGGFI
                     GADRVLKEIASGAKRKRVGIKPEGRAPAREGTVVTDAGGRQIGTITSGGFGPTANGPV
                     AMGYVETSFAKPGTAIQLIVRDKPMPAQIVNLPFVPHNYKR"
     regulatory      complement(2442..2539)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     regulatory      complement(2543..2632)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            2728..3690
                     /gene="ispH"
                     /locus_tag="PKV23_02000"
                     /gene_synonym="lytB"
     CDS             2728..3690
                     /gene="ispH"
                     /locus_tag="PKV23_02000"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HQX84234.1"
                     /translation="MSKPKLTVLLAAPRGFCAGVVRAIDIVERALALYGAPVYVRHEI
                     VHNKYVVDDLKSKGAIFVDELDEIPAGDRPVIFSAHGVPKAVPAAASARRLFQIDATC
                     PLVTKVHMEAQRHHAEGLEIILIGHKFHPEVTGTMGQLPSGAISLIETVSDAETLVVK
                     NPEKLAYVTQTTLSIDDTRDIIVALQRRFPGIRGPLREDICYATTNRQEAVKSIAGRI
                     DLLLVVGAPNSSNSQRLVEVGENNGCARALLVQRGTDLDWSQLQNLNTIGITAGASAP
                     EILVNEIIDAFRAHYEVTVENVVLREENVSFKLPRELRAAAAAS"
     gene            3687..4130
                     /gene="rnhA"
                     /locus_tag="PKV23_02005"
     CDS             3687..4130
                     /gene="rnhA"
                     /locus_tag="PKV23_02005"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006330641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HQX84235.1"
                     /translation="MTANKVIVHTDGACSGNPGPGGWGAILAFNGTRKELSGGEAETT
                     NNRMELMGAIAALESLKRPCKVEMHVDSNYVKDGITKWVHGWKRNGWKTADKKPVKNV
                     ELWQRLDAALATHDISWHWVKGHAGHNENERADELARAGMAPFKR"
     gene            complement(4169..>4562)
                     /locus_tag="PKV23_02010"
     CDS             complement(4169..>4562)
                     /locus_tag="PKV23_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013896644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HQX84236.1"
                     /translation="FAGRKVVLFALPGAFTPTCSRNHLPGFLANLDAIKAKGVESVVC
                     VTVNDAHVVDAWAEQTGAKGKIIMLADDNGAFTKAVGMNRFYPDNGMGERSRRYSMIV
                     DNGVVRQINLEEKPGVNVSGADTILEQL"
BASE COUNT          905 a         1311 c         1458 g          888 t
ORIGIN      
        1 cgaggtagcc cgcgtgcggg ccgccgtagc ccatgggcac gccgaagcgc tgggccgagc
       61 ccacggcgat atcggcaccc agttcaccgg gcggcgtgag caaggcaaga gccagaaggt
      121 cggttgccat gatggccatg gcgccggcgc cgtggagctt gtcgatcacc ccgcgataat
      181 ccctgatttc accggatgag ccggggtagg acaggagtgc ggcaaagacg gtttccggct
      241 tcaggtcctt ggcggggtcg ccaatgatga tctcataacc aaaggcgcgg gcccgggtct
      301 ggatgacgcc tatcgtctgc ggatgggtgt cgcggtcaac gaagaaggca tttgcagttg
      361 tcttgaccag gcgcttggcc atggccatgg cctcggcggc ggcggtggct tcatcgagaa
      421 gcgaggcatt ggcgagctca aggccggtga gatcaacgat tacctgttga aaattgagca
      481 gcgcctcgag gcggccctgg ctcacttcag cctgataggg tgtgtaagcc gtataccagc
      541 cgggattctc cagcacattg cgcaggatca cctttggcgt aacagtgccg taataaccca
      601 tgccaatcat cgaggtgaac acctcgttgc ggtccgccat ccggcgcaga taggagagcg
      661 cagtgcgctc cgccttggcc ttgggcaaat ccagcggccg cgccatgcgg atttccttgg
      721 gcaccacctt ggcaataaag tcctcaagcg acttggcgcc cacttcccgc agcatgtcgg
      781 tggtttcggt ttcgctgggg ccgatatggc ggcggataaa attggcgccg ggttcgaggt
      841 cggcaaggct ggcacgggac atcaatgaac tcctcagaga cttgcaacaa atttgtcgta
      901 ggcggccttg tccatcagct cgttgagctc agccggattg gagattttaa ctttcatgaa
      961 ccagccatcg ccttcggcgc cgcggttgac gagagccgga tcgccctcaa gttccttgtt
     1021 cacctcgacg acttcgccgg tgacgggggc atagacttcg ctggcggctt tgacgctttc
     1081 aaccacggcg gcatcgccac ccttggcaac cttcttgccg acggcgggca attcaacaaa
     1141 caccacatcg cccaactgct cctgggcgtg attggtgatg ccgatggtag cgatgtcacc
     1201 ggcaacgctt acccactcat ggtctttcga aaacttgacg gacataggcg atctcctcag
     1261 cgtttataat tatgggggac gaagggcagg ttcacgatct gggcgggcat gggcttatcg
     1321 cggacgataa gctggattgc cgtgcccggc ttggcgaatg aagtctcgac atagcccatg
     1381 gccacagggc cattggctgt agggccgaag ccgccggagg tgatggtgcc gatctggcgg
     1441 ccgcctgcgt cagtgacgac agttccctca cgggcggggg cgcggccttc cggcttgatg
     1501 ccaacgcgtt tgcgtttggc gcccgatgcg atctctttca gcacccggtc cgcaccaata
     1561 aagccacctt cgttccggcg gcgcttctgg attgaccaga ggagatcgcc ctcgacgggg
     1621 ctggtgcttt cgtcaatgtc gtggccgtaa aggcaaaggc ccgcttcgag gcgcagtgaa
     1681 tcgcgcgccc cgaggccgat gggtttcaca tccggattag ccagcagaag ctgccagagc
     1741 cgcaccgcgt ccttgtcgaa gatggaaatt tcaaagccgt cctcgccggt atagccggag
     1801 cgcgagacat gggcctcgat gccgtcaatg gaaaccgcgg cgtagtgcat gaaaggaata
     1861 tcgcgcacgg cgggtacaaa ttctgcaagt gctgcttcag ccttcggtcc ctgcaacgcg
     1921 acgagcgcca tatcagcgcc gcgctggagt ttcacatggg cgggtaagtg tctttcgata
     1981 tgggcaaagt cgtcatcctt gcgcgaggca ttgcaaacga gatagagcca gccctcgtaa
     2041 cccgcataag ccgagcgggt gatcatcaga tcatcgagaa tcccgccttc cggattgagg
     2101 aactgcgagt agcggatctg gtggggcttg aggctggcga tatcggcggg aaccagcttt
     2161 tccatggcgg cggcggtggt cgcgtggtcg gggcccacga gaaatgcctg gcccatgtgg
     2221 gagacgtcaa agaggcctgc actggttcgg gtccagttat gctcggtgag aataccggtg
     2281 ggatattgca cgggcatctc atagccggcg aagggcacga gcttggcgcc cagagcggca
     2341 tgctcgccgt agaggatcgt gcgcttgagg gcggtttcgt tcatttgaac tcctgacaat
     2401 tgacgacagg cccagcgcaa ggtttgcgca tggcctgccc cctctgtcag agaacctgag
     2461 agttttcgcc cgaaccgtca ctccgacacg gccagacttg ctcctacggt gaggggtgca
     2521 gcacccctgc tctccagagt tccaaaacgc ggcggtcctt ttgcctgaga gtttccgggg
     2581 cggttgctcc ttcggcgccg gggttgcccc cgccgtctcc cgcttggcgt tttacgcgat
     2641 ctgaagggaa ggtaggagga tggctggcaa aaggcaaggg tcgcagggtt tcttgacaac
     2701 gcgccccgcc cctcgcatgt aggcgccatg tcgaagccaa agctcaccgt tctgctggcg
     2761 gcaccacgcg ggttttgcgc cggggtggtg cgggccattg atattgtcga gcgggccctg
     2821 gcgctttacg gggcgccggt ttatgtgcgc cacgagatcg tgcacaacaa atatgtggtc
     2881 gatgacctca agtccaaggg cgcgatattc gtcgacgagc tggatgagat tccggctggc
     2941 gacaggccgg tgattttttc ggcgcacggg gttcccaagg cggtgccggc ggccgccagc
     3001 gcgcgccggc ttttccagat tgatgccacc tgtcccctgg tgaccaaggt tcacatggag
     3061 gcccagcgcc accatgcgga agggctggaa atcatattga tcggccacaa gtttcatccg
     3121 gaggtgacgg gcaccatggg gcaattgccg tcgggcgcca tcagcctcat cgagacggtg
     3181 tccgatgcgg aaacgctggt ggtcaaaaat ccggagaagc tggcctacgt cacccagacg
     3241 acgctttcaa ttgatgacac gcgcgacatc atcgtggccc tgcaacggcg ctttcccggg
     3301 atacgggggc cgttgcgcga agacatctgc tatgccacca ccaaccggca ggaagcggtg
     3361 aaatccattg ccggcaggat tgacctcttg ctggtggtgg gggcgccaaa ctcatccaat
     3421 tcccaacgcc tggtggaagt gggcgagaac aatggctgcg cacgggccct gctggtgcag
     3481 cgcggcactg acttggattg gtcgcagctt caaaacctca acacgatcgg gattaccgcg
     3541 ggcgcttcag cgccggaaat cctggtcaac gaaatcatcg acgccttccg ggcgcattat
     3601 gaggtgacgg tggagaatgt ggtgctgcgc gaggaaaatg tttcgttcaa actgccgcgc
     3661 gaactgcgcg ccgctgctgc cgcctcatga cggccaacaa ggtcatcgtg catacggacg
     3721 gcgcgtgttc gggaaatccc ggccctgggg gctggggcgc cattcttgcc ttcaacggca
     3781 cgcgcaagga gctctccggc ggcgaagcgg aaaccaccaa caaccgcatg gaactgatgg
     3841 gcgccatcgc ggcgcttgag agcctcaagc ggccctgcaa ggtggagatg catgtggatt
     3901 cgaattatgt gaaagacggc atcaccaagt gggttcacgg ctggaagcgc aatggctgga
     3961 aaaccgccga caagaagccg gtgaagaatg tggaactctg gcaaaggctg gatgcggcgc
     4021 tggcaaccca tgacatctca tggcactggg tgaaaggtca tgcgggccac aatgaaaatg
     4081 aacgggccga tgaactggcc agggcgggga tggcgccgtt caagcgctag aagtaaattc
     4141 ctctcccccc aaggggcaag gaacattttt atagttgctc caggattgta tcagcacccg
     4201 atacattgac gccgggcttt tcttccaggt tgatctggcg caccacgcca ttgtcgacga
     4261 tcatggaata gcgccttgag cgttcgccca ttccgttatc gggataaaac cggttcatgc
     4321 cgacagcctt ggtgaaggcg ccattgtcat cggccagcat gatgatcttg cccttggcgc
     4381 ccgtttgctc ggcccaggcg tccaccacat gggcatcatt caccgtcacg cagaccacgg
     4441 actcaacgcc cttggccttg atggcatcga gattggcgag gaaccccggc agatgatttc
     4501 ggctgcaggt gggagtgaag gctcccggca gggcaaagag cacgaccttg cggccggcga
     4561 aa
//
LOCUS       DAORVN010000064         4510 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_14169_length_4510_cov_3.705724, whole genome shotgun sequence.
ACCESSION   DAORVN010000064 DAORVN010000000
VERSION     DAORVN010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 4510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4510
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14169_length_4510_cov_3.705724"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            130..879
                     /locus_tag="PKV23_02015"
     CDS             130..879
                     /locus_tag="PKV23_02015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX84237.1"
                     /translation="MKKNVSGQFVGVQMVAATDGSAFTGAVTVSVTGDAGTQATGSVG
                     SGACTHEGNGYHTYAPAQAETNYDLVAFTFTGSGAVPATVQIYTSYPQTGDSYARIGA
                     PAGASVSADVAAVKVDTAAILVDTGTTLDGKIDAIDDYIDTEVAAIKAVTDALGATAA
                     GRLQTAVLAEQTGTIDTAAFSPTTTEFEADDITEATADHYNGRVIIFTSGALQYQATS
                     ISDYALSGGRGHFTVPALTEAPANNDTFIIV"
     gene            1097..1480
                     /locus_tag="PKV23_02020"
     CDS             1097..1480
                     /locus_tag="PKV23_02020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX84238.1"
                     /translation="MSITDNASISEGEKFAAFLDSDNVMWLWQIDPCDQAEVTTLVVT
                     SSTLHRCSVRVSFRTSTATALPFRLYSIRPYLKIQKQDVPYRYEAAVEARVTRPSIRV
                     TWTGGPFVMDTIQLRLKALKNQVKG"
     gene            1482..2210
                     /locus_tag="PKV23_02025"
     CDS             1482..2210
                     /locus_tag="PKV23_02025"
                     /inference="COORDINATES: protein motif:HMM:NF019126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HQX84239.1"
                     /translation="MADLSTTNSTYSTGGTDTWTSQVANVSLYRAQHVNGLASAILQI
                     EAALGSGSTLPGSLSSLAARLAVQVGSDGIIIPPGAGMDFFGSVAPTGWLFRDGSAVS
                     RVAYPALFAAIGTTYGAGDGSTTFNVPDDRHRVTMGAGTGARDGESGSGVISGGTALA
                     AKSIGEWGGASTVLLTGNQSGVQAHAHTVPTATDGIGAGATIANAYDASPASTVSTAS
                     QSGDALEAHTNEMTYLVCNKIIKT"
     gene            2219..2842
                     /locus_tag="PKV23_02030"
     CDS             2219..2842
                     /locus_tag="PKV23_02030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX84240.1"
                     /translation="MALTSFFSSGGTKQTSNSGFLEGKDKFGASGRAFDGLRVLSNDT
                     QEQFQAPLPQFNMSSSIPGLFQEQEGAAQAFANKMFANASAGGALRGQFSINNTPGIV
                     GSAITNMGATLLPMISQNLQNQMLIPEQIRAQRVQNAMSPLQALIAGLGSTSSGQSAG
                     PGLGYQSGNNFLQSFGSGLGSSLASVASAGLGGGGLSGMAAGIRGKT"
     gene            2842..>4510
                     /locus_tag="PKV23_02035"
     CDS             2842..>4510
                     /locus_tag="PKV23_02035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX84241.1"
                     /translation="MAESFAFDQNQRRNFDQWLTSDDASEVTPDALYNVLGVAPGMGA
                     FPAKEELQPGFLDKFLDAFGVQTDKVKSLKTLSLKQKQLEPFVNEMLKLKAVDLIHRN
                     AQLRAAPRTTDDAPAVSRTLGFGGGESQGLQPAPTLGGDPAMAGMPLGASSANAAAGP
                     QISPPDTSMPQPLSQGGRFMTPDAAISTPIPLGPQAQQFDYQGRLVPPMPQEELIPQP
                     IRPEPIAAPLPRATMPQQAPRQLKDFERFQAAQMLQSQATGHLVSDDSGKLVPASFMS
                     ADQRLLEAPQQQELARIYAGEQPSGTVRLPAAPLMAALKGKQDQTLQGLSAPIKDYLQ
                     AKGLKPTPDNIDQARTATRAEKIDDEKTAIRFKEALQAGSQKTRDFLASKTGKLFLAD
                     AKPAEIERAIKDATNNDISEHARKAMDSFNLGGLDGGEKEKLAGLQQILHVTDRLKNE
                     FTPAERREFVGYFQLPVNRLSQIIQANPRFAKFDALVNREGIAAFETGGKALTAQEAA
                     IIFGFLPNAKEWSPENFEAKLLEADDYGRSKIHTIVDIATTNRRDMGK"
BASE COUNT          986 a         1359 c         1335 g          830 t
ORIGIN      
        1 gactatgagt tggagcaata gcacgtcaca ggcgtgggcc attgccgctg ttcccgtcaa
       61 gcccgctgca gctggcagct ttcaggccgc gtggaccatt ggagcgaaca ccgtcatttt
      121 aggagccgca tgaaaaagaa cgtcagcggg caatttgtgg gagttcaaat ggttgccgct
      181 acagacggta gcgccttcac tggagccgtc accgtctccg tcaccggcga tgcaggcacg
      241 caagcaaccg gctcagtcgg ctctggggcc tgcacccatg agggtaacgg ctatcacacc
      301 tacgcacccg cgcaggctga aaccaattac gatcttgtgg ccttcacctt taccggatcg
      361 ggggcggttc cggcgaccgt gcaaatctat accagctatc cgcagaccgg cgacagctac
      421 gccagaatcg gcgcaccggc tggggccagc gtgagtgctg atgtcgcagc ggtgaaggtc
      481 gataccgccg ccatcctggt tgataccggg accacgctcg acggcaagat cgacgccatt
      541 gatgactata tcgacaccga agtggccgcg attaaggcgg tgacggatgc cctcggggcg
      601 actgccgcag gccgattgca aacggcggta ctggctgaac agaccggcac gattgacaca
      661 gcggcctttt cgcccaccac gaccgaattt gaggccgacg acatcacgga ggcgaccgcc
      721 gaccactaca acgggcgggt cattatcttc acgagcgggg ccttgcagta ccaagcaacc
      781 tcgatcagcg attatgcgct gtccggagga agagggcatt tcacggtccc ggcgttgacg
      841 gaagctccgg cgaataacga cacgttcatt atcgtatgac atggcgacaa ccagacttcg
      901 ctctacagga accccaggca agccgtatgg caccttcgcg gccaaactca gcgcgtcaac
      961 gcctcccgtc acgattcccg cacagggcgg cattatttcc tttacgcgct ccctggttcc
     1021 acgcccgatc attgccccgc ccttactcga ccaggacggc gggtattgga ggccgtctat
     1081 cagcaatgac ggcattgtgt ctattaccga taacgcctca atcagcgagg gtgagaaatt
     1141 tgccgccttt ctcgattccg acaatgttat gtggctctgg cagattgacc cctgcgacca
     1201 agccgaagtt accacgctgg tggttacaag ctcgacgctc caccgctgca gcgtgcgggt
     1261 tagtttcagg acgagtacag ccacggcgct cccgttccgg ctctacagca tccgccccta
     1321 cctcaagatt cagaagcagg atgtgccgta tcgctatgag gcggcagtgg aggcgcgagt
     1381 caccaggcca tctatacgcg tcacatggac cggaggcccg ttcgtgatgg acaccattca
     1441 gttacgactc aaggcactca aaaaccaggt gaagggatag catggccgat ctgtcaacca
     1501 ccaattcgac ctacagcacg ggcgggaccg atacatggac gagccaagtg gcgaatgtca
     1561 gcctgtaccg cgctcaacac gtcaacgggc tggcctcggc catcttgcag atcgaggccg
     1621 cgctggggtc cggatcgacg ctgccaggct cactcagttc gctcgccgct cgcttggccg
     1681 tacaggtcgg ctccgatggc atcatcattc cgccaggggc cggaatggat ttcttcgggt
     1741 ccgtggctcc aactgggtgg ctctttcgtg atgggtctgc cgtgagccgc gtagcgtacc
     1801 ccgccctgtt tgcggccatc gggacgacct acggggccgg ggacgggtca accaccttca
     1861 atgtcccaga tgaccggcat cgcgtgacca tgggcgctgg caccggcgcg agagacggcg
     1921 aaagcgggtc cggcgtcatt tccggcggaa cggcgctcgc cgcgaaaagc attggcgaat
     1981 ggggcggggc gagcacggtg ctgttgactg gcaatcaaag cggtgtgcag gcccacgcgc
     2041 atacggtgcc gaccgctacc gatgggatcg gcgcaggggc cacgattgcg aacgcctatg
     2101 acgcctctcc cgcgtctacg gtgtccacgg cttcacagag cggagacgcg ctagaggcgc
     2161 acacgaacga gatgacgtac ctcgtgtgta ataaaatcat caagacctag gaggcgctat
     2221 ggccctgacc agctttttca gttccggagg aaccaaacaa acctcgaaca gcgggtttct
     2281 tgaaggcaag gacaaattcg gggcctcggg gcgtgcgttc gatgggttgc gtgtcctctc
     2341 gaacgatacg caagagcagt tccaggcacc gctcccacaa ttcaacatgt cctcgtccat
     2401 ccccggcctg ttccaggagc aggagggagc cgcgcaggcc tttgcgaata aaatgtttgc
     2461 caacgcctca gccggtggag ccttgcgggg gcagttcagc atcaacaaca cgcctggcat
     2521 tgtgggctca gccattacca acatgggcgc gacgttgctc ccgatgatta gccagaattt
     2581 acaaaatcag atgctcattc ctgagcaaat ccgggcgcaa cgcgtgcaga acgccatgag
     2641 cccattacaa gccttaattg cagggctagg cagtacatct tccggacaat ctgcaggccc
     2701 agggcttggg tatcagtccg gcaacaactt tctgcaaagt ttcgggagcg ggctaggtag
     2761 cagccttgcg tctgtggcct ctgccggatt gggcggcggt gggctctctg gcatggccgc
     2821 aggaataagg gggaagacct aatggcggaa tcattcgcgt tcgaccaaaa ccagcggcgt
     2881 aatttcgacc aatggttgac ttctgacgac gccagtgaag tcacgccaga cgcactctat
     2941 aacgtcttag gggttgcgcc tggtatgggg gccttcccgg ctaaggaaga attgcagcct
     3001 gggttcctcg ataagttcct cgatgcgttc ggggtgcaga ccgataaggt caagtcgctc
     3061 aagacgctct cgctgaagca gaagcagcta gagccgttcg tcaatgaaat gctcaagctg
     3121 aaagcggtgg atctgattca ccggaatgcg caattgcgag cggccccacg caccaccgac
     3181 gacgcgccag ccgtgagccg cacgcttggg tttggtggcg gcgagagtca aggccttcaa
     3241 ccggccccta cgctcggcgg cgatccggcc atggccggga tgccgctggg agcctctagc
     3301 gccaatgcgg cggcaggacc acagatcagc ccgcccgaca cgtccatgcc gcaaccgctc
     3361 tcacagggcg ggcgattcat gacgcctgac gcagcgatct ctacgcccat tccgcttggt
     3421 ccgcaggccc aacagttcga ctatcagggc cgactcgttc cgcccatgcc acaagaagaa
     3481 ttgattccgc aaccgatccg cccggagccg attgccgccc ctctgccacg cgccaccatg
     3541 ccgcaacagg ccccgcgtca actgaaagat tttgagcggt ttcaggccgc gcagatgttg
     3601 cagtcgcaag cgactgggca tctggtttcg gacgactccg gcaagcttgt gcctgcgtcg
     3661 ttcatgagcg ccgatcagcg gttgcttgaa gcgccacagc agcaagaatt agctcgcatt
     3721 tatgcaggtg agcagccatc cggaacggtt cgcctcccag cagctccact catggctgcg
     3781 ttgaagggga agcaagacca gacgttacag gggctcagtg ctcctattaa agactatctc
     3841 caagcgaagg gattgaagcc gacgcctgac aatatcgacc aggcccgcac ggcgacccgt
     3901 gccgaaaaga tcgacgatga aaagacggcg atccgattca aggaagcctt gcaggctggc
     3961 agtcaaaaaa cgcgtgactt cctggcgtca aagaccggca agctgtttct ggccgatgcc
     4021 aaacctgccg aaattgaacg ggccattaag gacgcgacca acaacgacat tagcgaacat
     4081 gcccgcaagg ccatggacag ctttaacctc ggcggattgg acggcgggga aaaggaaaag
     4141 ctggctggct tgcagcaaat cctgcatgtc accgaccggc tcaagaatga gtttacccct
     4201 gccgaacggc gggagttcgt cggctatttc caattgccgg tcaaccggct ctcgcagatt
     4261 attcaggcga acccgcgctt tgccaagttt gatgcgctag tcaaccgtga aggcattgcg
     4321 gcctttgaga ctggtggtaa ggccctcaca gcgcaggagg cagcaatcat ctttggcttc
     4381 ctgcccaacg ccaaggaatg gagccctgag aatttcgagg ccaagctgtt ggaggccgat
     4441 gactacggac gctcaaaaat ccacacgatt gtggacattg ccacgacgaa ccgccgcgac
     4501 atggggaaga
//
LOCUS       DAORVN010000065         4494 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_14257_length_4494_cov_2.735751, whole genome shotgun sequence.
ACCESSION   DAORVN010000065 DAORVN010000000
VERSION     DAORVN010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 4494)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4494)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4494
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14257_length_4494_cov_2.735751"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..508)
                     /locus_tag="PKV23_02040"
     CDS             complement(<1..508)
                     /locus_tag="PKV23_02040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX84242.1"
                     /translation="MNYTISFAPLLPLLWLSILGMAGLVLIAVSAFARTRGWWLRGLA
                     MALLLASLSNPTLRHEDREALNDIAVAVIDRSQSQLAGARMQQADEAEAALKAATATL
                     TNTELRVVTVQSGLNPQEDGTRLFTALNRALGDIPPERFAGALLVTDGQVHDVPPDAS
                     KSGINGPLH"
     gene            complement(510..3290)
                     /locus_tag="PKV23_02045"
     CDS             complement(510..3290)
                     /locus_tag="PKV23_02045"
                     /inference="COORDINATES: protein motif:HMM:NF025086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HQX84243.1"
                     /translation="MMAFLQALTFTTPVALAGLLLLPVIWWLLRFTPPKPQTVKFPPL
                     RLLLELVSKQEQPDKTPWWLMLLRLAIAALVILGVSHPFYAPGKTAAGTSAPLLIIVD
                     DTWAAAKDWTLRRIMLNEIVAEARENDVTLTLATTAPSAREADIVARDADAMLKQIAA
                     LEPKALGPDRAKLLARLKTGFAAAASLHVVWLSDGLDQTSATAFAEGLAGLAGGSAQV
                     DAILPDAASLPLALAAPSAEGGQFKVHLLRSPSAGLRQANIRAVAANGRSLADVRLEF
                     AGNAAEAEAALDLPLELRNEVQRLEILGERNAAATYLFDDRWRRKTIALQSGSSLEDA
                     QPLLSPLYYVSRALEPYAQISEPEDAAQLKEQLEAGLSMLVLADIGVIPAETADAINP
                     WLDRGGVLLRFAGPRMAGAQDELVPVTLRSGGRALGSALSWEEPQNLQAFPDAGPFAG
                     LPLDLNVAVSRQVLAEPDMDLPGRVWASLADGTPLVTARRQGKGLIVLFHVTANAEWS
                     NLPLSGLFVEMLRRINDLAPGAGGGAAAGASSGDDRAAAFVPRLALSAAGELTEPLPD
                     SKPIPPAAMENAKPTPETPAGLYNRGSAERAINLLPDRQAMSAITALPAGVTLRSFAT
                     APRQPLAPVLFGLAFLLFLGDTLAALVLGGGWHRLRGMAAALVVVLVIPNDPAMAQQT
                     DDYAQKATEETRLAFVRTGDDTVDSISEQGLAGLGLILTDRTSVTPGSPIGVNIETDE
                     IVFFPLVYWPVLDGAAVPTPATLAKMDVYMKNGGTIFFDLRDDAMGLESLSGAATGAT
                     DALRRILAKLDIPPLEPVPENHVLTKAFYLLRDFPGRYARGQLWVERSDSTATNNVDG
                     VSSIIIGSNDYAAAWALDDNSNPLYAVIPGNDRQRELAFRTGINIVMYALTGNYKADQ
                     VHIPALLERLGQ"
     gene            complement(3287..4189)
                     /locus_tag="PKV23_02050"
     CDS             complement(3287..4189)
                     /locus_tag="PKV23_02050"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HQX84244.1"
                     /translation="MKTIPSLVLNERANAASQTLPPLLVEAERIAATVMQGIHGRKRS
                     GPGESFWQYRPYGFGDSTSRIDWRKSARSSHVFIRENEWEAANTLWLWASPSNSMDFK
                     SHLSKSTKRDRAYLTALAMASLALRAQERVGLIGSPHKASHARGALVPMATWLLRNEG
                     ASLPQSQHLQKFSSVVLLGDFLEKPEDIAATVRSLASRGVSGHIVQVNDPAEETLPYD
                     GRIEFRAMDGPLKYLAGKTELLRDAYGEKFAAQREAVRRIAHGVSWTFTLHHTDQSPA
                     KLLMMLHGLVGGAKSRAFDLGGIA"
     gene            complement(4202..>4494)
                     /locus_tag="PKV23_02055"
     CDS             complement(4202..>4494)
                     /locus_tag="PKV23_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019198677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HQX84245.1"
                     /translation="VTDAILRVVRAARPGEEASPEINHGVAWGPSPRASQALMLGIRA
                     RALLQGRLSPSIDDVAELIGPVFKHRMALNFTARAEGETIEAIIARQKNLLA"
BASE COUNT          825 a         1291 c         1575 g          803 t
ORIGIN      
        1 cgtgcagcgg gccgttgata ccgctcttgc tggcatcagg aggaacgtca tgtacctggc
       61 catcggttac caatagtgcg ccggcaaagc gctcgggcgg gatatcgccc agggcgcggt
      121 tcagggcggt aaacagcctt gtgccatcct cctgcggatt gaggccggat tgcacggtca
      181 ccacgcgcaa ctccgtattg gtcagggttg ccgtggcagc cttgagggcg gcttccgctt
      241 catcggcctg ttgcatgcgc gcgcccgcca gctggctttg gctgcggtcg atcacggcga
      301 cagcaatgtc attgagggct tcgcggtcct cgtggcgcag ggtgggattt gaaaggcttg
      361 caaggaggag cgccattgcc agcccgcgca accaccagcc gcgggtgcgg gcaaaggctg
      421 aaaccgcgat cagcaccagg ccagccatgc caagtattga aagccaaagc aggggaagca
      481 agggggcgaa tgaaatggtg tagttcatgc tactgcccca gcctttccag cagggccgga
      541 atgtggacct ggtcggcctt gtagtttccg gtgagcgcat acattacgat gttgatgccg
      601 gtgcggaacg ccagttcgcg ctggcggtca ttgccgggga tcacggcata gagcggattg
      661 ctgttgtcat cgagggccca ggcggcggca tagtcgttgc tgccgatgat aatggaactc
      721 acgccgtcaa cattgttggt ggcggttgaa tcgctgcgtt ccacccagag ctggccgcgc
      781 gcgtagcggc cgggaaagtc gcgcaggaga taaaaggcct tggtcagcac gtggttttcc
      841 ggcaccggct caagcggtgg aatatcaagc ttggcgagaa tgcggcgcag cgcatcggtt
      901 gccccggtgg ccgctcccga caggctctcg aggcccatgg catcatcgcg gagatcgaaa
      961 aaaatggtgc cgccgttctt catgtagaca tccatcttgg caagcgtggc gggggttggc
     1021 acggcagcgc catccagcac cggccaatag accagaggga agaatacgat ttcgtcggtc
     1081 tcgatgttca cgccgatggg gctgccaggg gtgactgacg tccggtctgt cagaatcagg
     1141 ccaaggcccg ccaggccctg ctcgctgatg ctgtccaccg tgtcgtcacc ggtgcggaca
     1201 aaggccaggc gggtttcctc tgtcgccttc tgggcatagt catcggtctg ctgggccatg
     1261 gctggatcgt tgggaatgac gaggacaacg acaagggctg ccgccatgcc gcgcagccgg
     1321 tgccagccgc cgccaaggac aagtgcggcc agcgtatcgc caaggaatag caggaaagcg
     1381 aggccaaaga gcacgggcgc caggggctgg cgcggcgccg tggcaaagga gcggagagtt
     1441 acgccagcgg gaagtgcggt gatggcgctc atggcctggc gatcaggcag gagattgatg
     1501 gcgcgttcgg cgctgccgcg attgtaaagg ccggcagggg tttccggcgt cggctttgcg
     1561 ttttccatag cggctggcgg gatcggtttg gagtccggca gcggttcagt gagttcaccg
     1621 gcagcggaaa gtgcaaggcg cgggacaaag gcggcggcgc ggtcatcgcc gcttgatgct
     1681 ccggcggcag cgccaccgcc cgcacccggg gcaagatcat tgatccggcg gagcatttcc
     1741 acaaagaggc cggagagcgg caggttcgac cattcggcat tggcggtcac atggaagaga
     1801 acaatgaggc ccttgccctg gcgcctggcg gtgaccaatg gcgtgccatc ggcaaggctt
     1861 gcccataccc ggccgggcaa atccatgtcc ggttcggcca acacctggcg cgacacggca
     1921 acattcagat caagcggcag tccggcaaag gggccagcat cggggaaggc ctgcaagttc
     1981 tgtggttcct cccaacttaa ggcagaaccc agggctcggc cgccgctgcg aagcgtaaca
     2041 ggcacgagtt catcttgggc gccggccatg cggggaccgg caaagcgcag cagcacgcca
     2101 ccgcgatcaa gccagggatt gatggcgtcc gcagtttcgg cggggatcac gccgatgtcg
     2161 gccagcacca gcatggagag gccggcctcg agctgctctt tcaactgtgc ggcgtcttcc
     2221 ggctcggata tctgggcgta gggttcaagg gcgcgcgaga cataataaag cggcgagagc
     2281 aggggctggg catcttccag cgaactgccg gattggagcg caatggtctt gcgccgccag
     2341 cggtcatcga aaaggtaggt tgcggccgca ttgcgctccc ccagaatttc caggcgctgc
     2401 acttcgttgc ggagttcaag gggcagatcg agggccgctt cggcttcagc cgcattgccg
     2461 gcaaattcca gccggacgtc ggcaaggctg cggccattgg cggccacggc gcggatgttt
     2521 gcctggcgca acccggcgga tggagagcgc aggagatgga ccttgaactg gccgccttcc
     2581 gccgagggtg ccgcaagggc caggggcagg gatgcggcat ccggcaagat ggcatcgacc
     2641 tgggcgctgc cgcctgcaag gccggcaagg ccttcggcaa atgcggtggc cgaggtctgg
     2701 tccaggccat cgctgagcca gacgacatgc agtgaggcgg cggccgcaaa accggttttc
     2761 aggcgcgcca gaagtttggc gcggtccggg cccagggcct tgggttcgag tgctgcgatc
     2821 tgcttgagca tggcatcggc atcgcgcgcc acaatatccg cttcgcgggc agacggtgca
     2881 gtggtggcaa gcgtgagggt cacatcgttt tcgcgcgctt cggcgacgat ttcattcagc
     2941 ataatgcggc gcagggtcca gtccttggcc gccgcccagg tatcatcgac aatgatgaga
     3001 agaggggcgc tggttcctgc cgcggttttg cctggcgcgt agaagggatg ggacacgccg
     3061 agaatgacga gtgccgctat cgccaggcgc agcagcatga gccaccaggg cgtcttgtcg
     3121 ggctgctcct gcttgctcac caattcgagg agcaggcgca acggcggaaa ttttacggtc
     3181 tggggcttgg gcggggtgaa gcgcagcagc caccagatga ccggcagcag cagcagcccg
     3241 gcgagtgcta cgggcgtggt gaaggtcagc gcctggagga aggccatcat gcgatgccgc
     3301 ccaggtcaaa tgcccggctc tttgccccgc caaccaggcc atggagcatc atcaggagtt
     3361 ttgccggcga ctggtcggtg tggtgcaagg tgaaggtcca gcttacgcca tgggcaatgc
     3421 ggcgcacggc ttcgcgctgg gcggcaaact tttcaccata ggcgtcgcgc agcagttcgg
     3481 ttttgccggc gagatactta agcgggccat ccatggcgcg gaattcgata cgcccgtcgt
     3541 agggcagggt ctcttcggct gggtcattga cctgcacgat gtggcccgag acgccgcgcg
     3601 aagcgaggct ccgcacggtc gcggcaatgt cttccggctt ctcgagaaaa tcaccgagga
     3661 gcacgacgct ggaaaacttc tgcaaatgct gggactgggg caggcttgcc ccttcattac
     3721 gcaacagcca ggtggccatg ggcaccaggg cgccgcgggc gtggcttgcc ttgtgcggac
     3781 tgccgatcag gcccacccgc tcctgggcgc gaagggcgag gctcgccatg gccagcgccg
     3841 tgagataggc gcggtcgcgt ttggttgact tgctgagatg tgatttgaaa tccatggagt
     3901 tggatggcga agcccagagc cacagggtgt tggcggcttc ccattcattt tcgcggatga
     3961 agacatggga tgagcgcgcc gatttccgcc agtcgatgcg gctggttgaa tcgccaaagc
     4021 cataggggcg gtattgccag aagctttcgc cgggcccgga gcgcttgcgg ccatggatgc
     4081 cttgcatgac cgttgcggca attcgttcgg cctccaccag caggggtggc aaggtttgtg
     4141 acgccgcatt ggcgcgttca ttcaagacca gtgacggaat ggtcttcatg gtcggcgctc
     4201 gctacgcgag aagatttttt tgccgtgcaa tgatggcctc aatggtttcg ccctcggcgc
     4261 gggcggtgaa gttcaatgcc atgcggtgct tgaagacggg gccgatgagt tcggccacat
     4321 cgtcgatcga ggggctgaga cggccctgca gcagggcgcg ggcgcgaatg cccagcatca
     4381 gggcctggct ggcacggggc gatggccccc aggcaacgcc gtgattgatt tccgggctgg
     4441 cctcttcacc cggcctggcg gcgcgcacga cacggaggat ggcgtcagtt acct
//
LOCUS       DAORVN010000066         4457 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_14498_length_4457_cov_3.024761, whole genome shotgun sequence.
ACCESSION   DAORVN010000066 DAORVN010000000
VERSION     DAORVN010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 4457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4457
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14498_length_4457_cov_3.024761"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(110..982)
                     /locus_tag="PKV23_02060"
     CDS             complement(110..982)
                     /locus_tag="PKV23_02060"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HQX84246.1"
                     /translation="MHVSSNLRGIACMILASGTFVANDSFMKLVLADTPPLQVLLMRG
                     MAATLWCLPLVLIMGYGRDIPRVLNPWVLLRAAFEILAVVSFILALNHMPIGDITAIF
                     QTSPLLVVAGVSLIWGERIGGLRMMLIALAITGALLIAQPGSSTASPYAAFGILTAIG
                     SALRDIVGKKVPHDIPGLVVALATIIAVMAVAGFITAFFETWVPPSGSNILMMLAAGL
                     FLVGGHTFVFLAFRFARAAAVAPFYYTFTLWAVLSGLIVFGDVPNWLSVLGMLLILTS
                     GLASVAMDQRWLKQ"
     gene            complement(1059..2231)
                     /locus_tag="PKV23_02065"
     CDS             complement(1059..2231)
                     /locus_tag="PKV23_02065"
                     /inference="COORDINATES: protein motif:HMM:NF014803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX84247.1"
                     /translation="MLNATTNLTFESAAAASLALKPDAPLFCFSAPQLQARARIFLEG
                     FPGKVTFAVKANSSSQVIRRLADAGITAWDVASVQEMAKVGKAAPNARFHYHNPVKSR
                     REITDAFNLYKCRRYVVDCREELAKIADIIGPGKDIEIAVRFVLPRDRASSAHDFSTK
                     FGAPDHICVELLKQVVALGYTPLLTFHPGSQATDPNTYVRHIEAAARIARTADVEISM
                     LNVGGGFPANYVLSQAPEPPVFFDAIDKAVLKAFGKAKRPALECEPGRGLVATCMSLL
                     TRVKLVCTDGDDIFINDGVYGSLMEYMQVPDLEPPFRVIRDGKLIDGPTKSWKVFGPT
                     CDPLDVLPHRLDILADLREDDYIEFGSLGAYGLATMTNFNGYGGNAIVNVDHALAL"
     gene            2349..2786
                     /locus_tag="PKV23_02070"
     CDS             2349..2786
                     /locus_tag="PKV23_02070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX84248.1"
                     /translation="MRLFVTLAFACLTLSGLPALAETAVPQNLGQMQLSFAPVVKTTA
                     PAVVNVYSKTLAAQDSAQGLFNDPFFRQFFGERGNFGRPRKQVENSLGSGVIVDARGL
                     IVTNNHVIRGARISAWFLPTSANSRQSCCWLMSAATWPFSKLK"
     gene            2699..3742
                     /locus_tag="PKV23_02075"
     CDS             2699..3742
                     /locus_tag="PKV23_02075"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HQX84249.1"
                     /translation="MVLADKREFEAKLLLADERSDLAILKIEVGDEELAALPFGDSDN
                     LEVGDLVLAIGNPFGVGQTVTSGIVSALARTQVGISDYQFFIQTDAAINPGNSGGALV
                     NMAGELVRINTAIFSRSGGSIGIGFSIPSNMVRTVVQSAESGTKILRPWSGANLQDVT
                     ADIAESLGFARPEGALVASLHPDSPLLAAGLKQGDVILALDGHPIENAKEFNYRVATS
                     QIGQASIIEYQRGGKAREASIKLVAAPETTDRAETLMEGKNPLQGMVVANLSPAVAEE
                     LGVPSSLSGVIALDIKGGVAKRFFRKGDILLEVNGVSIASVEDLEKAVSVDESYWEIQ
                     INRGGRKLQMTLR"
     gene            3742..>4457
                     /locus_tag="PKV23_02080"
     CDS             3742..>4457
                     /locus_tag="PKV23_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HQX84250.1"
                     /translation="MSTLFEAAGFGKEAPRPLADRLRPQKLTDVVGQEHLTGEDGVLS
                     RMLASGRIPSIILWGPPGTGKTTIARLLAGETGLAFEQMSAIFSGVADLKKAFEQARI
                     RREQGRGTLLFVDEIHRFNKSQQDSFLPYVEDGTITLVGATTENPSFELNGALLSRAQ
                     VLVLNRLDADAMTALVTRAEMFEKRPLPVDAQALSALIAMADGDGRYILNLVEEIFSS
                     IPEGALPLTSEALAKSVQKR"
BASE COUNT          968 a         1192 c         1444 g          853 t
ORIGIN      
        1 tgagacgaag gaggaaaatc ttgccgcgct gcttgaaatg gcagtcgatt tccaaggcaa
       61 gaggctgccg atcatgaagg cccttgggat cgcccaatag gacctgttcc tattgtttta
      121 gccagcgctg gtccatggcc acgctggcga ggccgctggt gaggatgaga agcattccca
      181 gaaccgagag ccaattcgga acatcgccaa agacgatcag gccggagagc acggcccaca
      241 gggtgaaagt atagtagaaa ggggcaactg ctgccgccct ggcaaagcgg aaagccagga
      301 acacgaaagt gtggccgccg accagaaaca atcctgctgc gagcatcatg agaatgttgg
      361 aaccgctcgg aggaacccaa gtctcaaaaa acgccgtgat aaacccggcg acggccatta
      421 cggcgataat ggttgccagt gcaaccacga ggcctggaat gtcgtggggc acttttttgc
      481 ctacgatatc gcgaagggcc gaaccaatgg ccgtaagaat gccaaaggcg gcgtagggcg
      541 aagcggtgga actgccgggc tgggctatca gcaatgcgcc ggtaatggct aatgcaatca
      601 gcatcatacg caggccgccg atccgttcac cccagatcag cgaaacgccc gcgaccacaa
      661 gcaggggtga cgtctggaaa atggcggtga tatcgccaat gggcatgtgg ttgagcgcca
      721 gaatgaagct gacgactgcg aggatttcga atgcggcgcg aagcagcacc caggggttga
      781 gaacgcgggg tatatcgcgg ccatagccca ttatgagcac caggggcagg caccagaggg
      841 tggcggccat gccccgcatc aggagcacct gcaggggcgg ggtgtctgcg agaacgagct
      901 tcatgaaact gtcgttggcc acaaaggtgc cgctggccag aatcatgcag gcaatgccgc
      961 gcaaattgga agaaacgtgc atattgagtg attaagtcag gcggctgaat gcggcaagat
     1021 cgccacccgc atggcagtga tgtccggctg cggcgctgct acagggcaag cgcatgatcc
     1081 acgttcacga tggcgttgcc gccataaccg ttgaaattcg tcatggtggc aaggccatag
     1141 gccccaaggg agccgaattc gatgtagtca tcctcacgca ggtcagcgag aatatcaagc
     1201 cggtgaggca gcacgtcgag ggggtcgcag gttgggccaa agaccttcca ggatttggtg
     1261 ggcccatcaa tcaactttcc atcgcggatc acccggaaag gcggctcgag atccggcacc
     1321 tgcatgtatt ccatgaggct gccatacacc ccgtcgttga taaagatgtc atcgccatcg
     1381 gtgcagacca gcttgacgcg ggtgagcagg gacatgcagg ttgccaccag tccgcggccc
     1441 ggctcgcatt ccagcgcagg acgtttcgcc ttgccgaagg ctttgagcac ggctttgtca
     1501 atggcgtcaa aaaacaccgg aggctcaggc gcctggctca gcacatagtt ggcgggaaag
     1561 ccgccgccga cattgagcat cgatatctcc acgtccgccg tgcgggcgat acgcgccgcg
     1621 gcctcaatgt ggcgtacata ggtattcggg tccgtggcct gggagccggg atgaaaggtg
     1681 agcagggggg tgtaaccgag ggccacgacc tgcttgagca gttcgacgca gatatggtcg
     1741 ggggcgccaa atttcgtgga aaagtcatgg gccgatgagg cgcgatcgcg gggcagcaca
     1801 aagcgcacgg cgatctcgat atctttccca gggccgatga tatcggcaat ttttgccagc
     1861 tcttcgcggc aatccacgac atagcgccgg catttgtaga gattgaacgc atcggtgatt
     1921 tcgcggcgtg actttaccgg attgtggtag tgaaagcggg catttggcgc cgctttgcca
     1981 accttggcca tttcttgaac cgaggccacg tcccaagccg ttatgcccgc atcggccagg
     2041 cgcctgatta cctgagatga ggaattggcc ttgaccgcga aggtgacctt gccggggaaa
     2101 ccctcgagaa aaatccgggc gcgcgcctgc aattgcgggg cgctgaagca gaacaggggt
     2161 gcatcaggct tcagggccag ggacgccgcc gcggcggatt caaaggtcag atttgtcgtg
     2221 gcattaagca tatcggggag ctttgcaaaa aaggtgtgtt tcggcgattc gtgctatatt
     2281 cttgtgacgt gcatcatatc gccaaagcgg gcgacttgat agaatagtct ggagaatttg
     2341 gagctgatat gcggttgttt gtaacattgg cttttgcctg tctcaccctg tccggcctgc
     2401 cggcgctggc ggaaacagcg gtgccccaaa atcttgggca aatgcagctt tcctttgcgc
     2461 cggtggtgaa aaccacggcg cctgccgttg tgaacgtcta ttcaaagacc cttgcggcac
     2521 aggattccgc gcagggactg ttcaacgatc cgtttttccg ccagttcttt ggtgaacgcg
     2581 gcaatttcgg acggccgcgc aagcaggttg agaactcgct gggttcgggc gtcatcgtcg
     2641 atgcgcgcgg tctgatcgtg accaacaacc atgtaatccg cggggcacgg atatccgcgt
     2701 ggttcttgcc gacaagcgcg aattcgaggc aaagctgctg ctggctgatg agcgcagcga
     2761 cctggccatt ctcaaaattg aagtaggcga tgaggaactg gcggcgctgc catttggcga
     2821 ttccgacaat cttgaggtgg gcgatctggt gctggcgatc ggcaatcctt tcggggtcgg
     2881 gcaaacagtg acgagcggca tcgtatcggc gctggcgcga acgcaagtgg gcatcagcga
     2941 ttaccagttt ttcatccaga ccgatgcggc gatcaaccct gggaactccg gcggcgcact
     3001 ggtgaacatg gcaggtgaac tggtaaggat caatacggcc atcttctcgc gctcgggcgg
     3061 ctccatcggc atcggctttt cgataccctc caacatggtg agaaccgtgg tgcagtcggc
     3121 ggaaagcggc acgaagattc tgcggccgtg gagcggggca aaccttcagg acgtgaccgc
     3181 cgacattgcc gagtcactgg gctttgcacg gccggaaggg gccctggtgg cgagccttca
     3241 tccggacagc ccgctgcttg ccgccggatt gaagcaaggg gatgtcattc tggctcttga
     3301 tggacatccg attgaaaatg ccaaggaatt caattaccgg gtggcgacct cgcagatcgg
     3361 gcaggcgtcg atcatcgagt atcaacgggg cggcaaggcg cgcgaggcca gcatcaagct
     3421 tgtggcggcg cccgaaacaa ccgatcgcgc cgaaactctc atggaaggca agaatcccct
     3481 gcagggaatg gtggtggcca atctttcacc ggcggtagcg gaagaactcg gcgtgccgtc
     3541 cagcctcagc ggcgttatcg cgctggatat caagggcggc gtggccaagc ggtttttccg
     3601 caagggcgat atcctgcttg aagtgaacgg cgtttccatc gccagcgtgg aagacctgga
     3661 gaaggctgtt tctgttgacg aaagctattg ggaaatccag atcaaccggg gcggtcgcaa
     3721 gttgcagatg acattgcgct gatgagcacg cttttcgaag cggcgggctt tggcaaggag
     3781 gcgccacggc ctctcgccga caggctgcgg ccgcagaagc tgactgatgt ggtggggcag
     3841 gagcacctga cgggggaaga cggcgttctc agccgcatgc tggcttcggg gcgcattccc
     3901 tcgataatcc tgtgggggcc accgggcacg ggcaagacga cgattgcaag gctgctggcg
     3961 ggtgaaaccg ggcttgcctt tgaacagatg tcggcgattt tttccggcgt ggcggatttg
     4021 aagaaggctt tcgagcaggc gcgcataagg cgcgagcagg gacggggcac gcttctgttc
     4081 gtcgatgaaa tccaccgctt caacaaatcg cagcaggatt cgtttctccc ctatgtggag
     4141 gacgggacga tcacgctggt tggggccacg acggaaaatc catcgtttga attgaatggc
     4201 gcacttctct cgcgggcgca ggttctcgtt ctgaaccggc tagacgccga tgccatgacg
     4261 gcgctggtga cccgggctga aatgttcgag aaacgcccgc tgccggttga cgcgcaggcg
     4321 ctatcggcgc tgatcgccat ggccgatggg gacgggcgct acattctcaa tctggtggag
     4381 gaaatttttt ccagcattcc ggaaggggcc ttgccgctga cctccgaggc cctggccaag
     4441 tcagtgcaaa agcgcat
//
LOCUS       DAORVN010000067         4427 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_14648_length_4427_cov_3.322507, whole genome shotgun sequence.
ACCESSION   DAORVN010000067 DAORVN010000000
VERSION     DAORVN010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 4427)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4427)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4427
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14648_length_4427_cov_3.322507"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..980
                     /locus_tag="PKV23_02085"
     CDS             <1..980
                     /locus_tag="PKV23_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007009547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="VC_2705 family sodium/solute symporter"
                     /protein_id="HQX84251.1"
                     /translation="QNTPGDVMNSWKMVSLAICMMAGTASLPHILMRYFTTPSVRSAR
                     RSVGWSLLFIFLLYFSAPALATLTKLQLLDPNLATSIIGKPFADVANLEWIKHWASVG
                     MLAVQDQNADGILQLNEFFMRPDIVVLATPEIAGLPFVISGLVAAGGLAAAMSTADGL
                     LLAIANALSHDLYYKIIDPSASTKRRLTVARILLLFIGAAGALVASLKLTGILGAVAW
                     AFDFACSGLFFPLVLGIWWKRANTAGAIAGMVGGFVSGSLYLYLVFNKIMDPWWGIDD
                     IRFGIIGMAVSVVAMVVVSLMTKEPSKEVQAMVDEVRVPSGKTILSSGH"
     gene            1055..1417
                     /locus_tag="PKV23_02090"
     CDS             1055..1417
                     /locus_tag="PKV23_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008837748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HQX84252.1"
                     /translation="MVELFEIFPWWVILIDYVLGLVMWTLIGRAAMGFFLPETSTFFF
                     MRFFVRSTDPVIRVFRPITPGFLIPQMVPLYVAWFFFMVRFYLMPWLLGYSVMGMLSF
                     PLESKVAAFIYKIFTPVP"
     gene            1441..3537
                     /locus_tag="PKV23_02095"
     CDS             1441..3537
                     /locus_tag="PKV23_02095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF294 nucleotidyltransferase-like
                     domain-containing protein"
                     /protein_id="HQX84253.1"
                     /translation="MKQEHPVATAATPLLSLTAIALDTETTSLDVNHARILEIGAIAI
                     LNGRLVPESHFASLVNPGAEIPRESVAIHGITDDAVKSAGSFAQVYKQYRGFAGAHVV
                     LGYAVGFDLAMLRSEHERAGLAWKAPRFLDVLDLARSLRPDLPNFSLETLAAWLGIAV
                     VDRHRALPDARLAAEVFLALLPRLRRNSIRTLAEAEDACRKSATSSGVAGWHEAAAPA
                     SSPVRVDSYPYRHRAKDVMSKPPVFAAPALNLREALALLVRRKVSSLFIAREKPGAPH
                     GIVTERDILRAIDRDAAKAFTKPVKSIATFPLESVSQEDFLYIAFGRMRRKKYRHMGV
                     VDADGELVGAITQRDLLRLQADEALAFADALGEAAGPAELAMVWRKLANAARSLLHEE
                     VDPRDIAGIISGEVRSLTARAATLAEKEVSAAFPKPKALRFAVMVLGSVGRGESLLAL
                     DQDNAIVFDAADEPEAEAWLLKLGQRMNAILDEVGVPLCKGGVMAGNPAWCKSAGQWR
                     KQVAHWLSRAEPQDILNADIFFDALPVHGDHGLVDDLRRDAISAASRTMAFLQLMDMS
                     AAKARPPVNWLGRFSLEEDGRMDLKMHGIMPIFSAARVLALRHALAEHSTAARLNALR
                     GKPGVLEERIDGLLEAHQIILRAILQQQLADIEQGIPPSNRVDPKKLHAPEKAHLMWA
                     LKQVPLVRDMLGVPVA"
     gene            3584..>4427
                     /locus_tag="PKV23_02100"
     CDS             3584..>4427
                     /locus_tag="PKV23_02100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HQX84254.1"
                     /translation="MLQSWMIFFFGLAYLGALFAVANWGDKPANRREPGSPRPFIYAL
                     SLGVYCTSWTYFGSVGIASRTGFDFLPIYIGPILVFALGWPLLQRIAEIAKRQNIASI
                     ADFLSARYGKSEALGALVAVVATIGVVPYISIQLKAVSQSLQTMITDPLFPEDIMPVM
                     QGADGLSMLVALTMGVFAILFGTRHIDTTEHQDGMILAISVESIVKLVAFIAVGLFVT
                     LTMMGGFEKLAAQVTADPHIYALLANGLSGTRWLTLTLLAAIAIILLPRQFHVAVVEN
                     SDEKQ"
BASE COUNT          738 a         1400 c         1294 g          995 t
ORIGIN      
        1 cgcagaatac gcccggcgac gtgatgaact cgtggaagat ggtgtctctt gccatctgca
       61 tgatggccgg caccgcatcg cttccgcata ttctgatgcg ctatttcaca accccatcgg
      121 tccgctcagc ccggcgctct gtcggctggt cgctgctctt catcttcctg ctgtacttct
      181 cggcgcccgc tcttgcgacg ctgacgaagc tgcagctgct cgatccgaac ctggcgactt
      241 ccatcatcgg caagccgttc gcggatgttg ccaatctgga atggatcaag cactgggcgt
      301 ccgtgggcat gctcgcggtg caggaccaga atgccgatgg catccttcag ttgaacgagt
      361 tcttcatgcg gcccgacatc gtagtgcttg ccacgcctga aattgctggc ttgcccttcg
      421 taatttccgg cctcgttgcg gctggcggcc ttgcggcggc catgtcgacg gccgacgggc
      481 tgttgctcgc catcgccaac gcgctctcgc atgatctcta ctataagatc atcgatccgt
      541 cggccagcac gaagcggcgc ctcacagtgg cccgcatcct gttgctcttc atcggtgcgg
      601 cgggtgctct cgtcgcgtcg ctaaagctta cgggaattct gggtgccgtg gcgtgggcct
      661 tcgactttgc ctgttccggc ctcttcttcc cgctggtact cggcatttgg tggaaacgcg
      721 ccaacaccgc cggcgctatc gccggcatgg tcggtggctt tgtctccggc tcgctctacc
      781 tctacctcgt gttcaacaag atcatggatc cgtggtgggg aattgacgac atccgcttcg
      841 gcatcattgg catggctgtc agcgttgtcg ccatggtcgt ggtcagcctc atgaccaagg
      901 agccttcgaa agaggttcag gccatggtgg acgaggttcg cgtgccatcg ggcaagacga
      961 tccttagttc gggtcattga agcaaaaaac agctagaatg caggggcgga attttccgcc
     1021 cctgttttat gttttatgat gcggaccttt tcacatggtt gagctgttcg agattttccc
     1081 gtggtgggtc atcctcattg actacgttct cgggctggtc atgtggacct tgatcgggcg
     1141 cgccgccatg gggttctttc tcccggaaac ctccacgttc ttcttcatgc ggttttttgt
     1201 gagatcgact gaccccgtca tccgggtatt caggccgata acgccggggt ttctgattcc
     1261 gcagatggtg cccctctacg ttgcctggtt cttctttatg gtccgctttt acctgatgcc
     1321 gtggcttctc ggctattcgg tcatgggcat gctatccttt ccattggaaa gcaaagtggc
     1381 ggcattcatc tacaagattt tcacgccagt gccgtaacca ttccgcggcc ggaggatttc
     1441 atgaagcaag agcatcccgt ggcgactgcg gcaacgcccc tgctgtccct gacagccata
     1501 gcgctggata ccgaaacaac gtcactggat gtgaaccatg cccgcattct ggaaatcggc
     1561 gccatcgcca ttctcaatgg caggcttgtg cccgaatccc actttgcctc gctggtcaat
     1621 ccgggcgcgg aaattccgcg tgagtcggtt gcaattcacg gcatcaccga tgatgcggtg
     1681 aagtcagccg gcagcttcgc gcaggtttac aagcagtacc gcggctttgc cggggcccat
     1741 gtggttctgg gatatgcggt gggctttgac cttgccatgt tgcgcagcga gcatgagcgc
     1801 gccggccttg cctggaaggc cccgcggttt ctcgacgtgc tcgatctggc gcgctcgctc
     1861 aggcccgacc tgccgaactt ttccctcgaa acgctggccg cttggctcgg cattgcagtg
     1921 gtcgaccgcc atcgtgccct gcccgatgcc aggcttgcgg cggaagtctt cctcgccctc
     1981 ttgccccgcc ttcgcaggaa ttccatccgc accctggcgg aagccgagga tgcctgccgg
     2041 aaatccgcca cctcttcagg cgtggcgggt tggcatgaag ctgcggcgcc agcgtcaagt
     2101 cccgttcgtg ttgacagcta tccctatcgg caccgggcca aggacgtgat gtcaaagccg
     2161 ccggtatttg ctgctcccgc cctgaacctt cgcgaagccc ttgccctgct cgtgcgccgc
     2221 aaggtgagtt cgctcttcat cgcgcgtgag aagcccggag ccccccacgg cattgtgacc
     2281 gaacgcgata ttctccgggc cattgaccgc gatgctgcga aggcattcac aaagccggtg
     2341 aaaagcatcg ctacattccc gctcgagagc gtttcccagg aagattttct ctacatcgcc
     2401 ttcggccgca tgcggcgcaa gaaataccgt cacatgggcg tggtcgatgc ggatggcgaa
     2461 ctcgttggcg ccatcaccca gcgcgatctg ctgcgcctgc aggccgatga ggccctggcc
     2521 tttgccgacg cgcttggcga agccgcagga cctgcagagc ttgccatggt ctggcggaag
     2581 ctggcgaacg ccgcgcgctc cctgctgcat gaggaggtgg atccccgcga cattgccggg
     2641 atcatttccg gcgaagtgcg aagcctgacg gcccgcgccg ccaccctggc ggaaaaggaa
     2701 gtgtccgcgg cttttccgaa accgaaagcc ctgcgctttg cggtgatggt gctgggctcg
     2761 gtcggccgcg gtgaaagcct gctggcgctg gaccaggaca acgcgattgt ctttgacgcg
     2821 gcagatgagc ctgaagctga ggcctggctc ctgaaacttg gccagcgcat gaatgccatt
     2881 ctggacgagg tgggcgttcc cttgtgcaag ggcggcgtga tggcgggcaa cccggcctgg
     2941 tgcaaatcgg cgggccaatg gcgcaagcag gtggcccatt ggctgtcccg ggccgaaccg
     3001 caggacatcc tgaacgccga catatttttc gacgccctgc cggtccacgg cgatcatggc
     3061 ctggtggatg acctgcgccg cgatgccatt tcagccgctt cccggaccat ggccttcctg
     3121 cagctcatgg acatgagcgc cgcaaaggcg cgcccccctg tcaactggct gggccgcttc
     3181 agcctggaag aagatggccg catggatctg aagatgcacg gcatcatgcc gattttttct
     3241 gccgcccgtg ttctggccct gcgccacgcc ttggctgaac attccaccgc cgccagattg
     3301 aacgcgctgc gcggcaagcc gggtgttctt gaggaaagga ttgatgggct actcgaggcg
     3361 caccagatta tcctgcgcgc cattctccag cagcagcttg ccgacatcga gcagggcata
     3421 ccgccttcga accgcgtcga cccgaagaag ctccacgcgc ccgaaaaggc ccatctcatg
     3481 tgggccctga agcaggtgcc gctggtgcgc gacatgctgg gcgttcccgt cgcctagata
     3541 gcccggcatt tccattcgca aggttttcgc ctaggctcgg cccatgctgc aaagctggat
     3601 gatcttcttc tttgggttgg cttatctcgg ggccctgttt gccgttgcaa actggggcga
     3661 caagcccgcc aaccgccggg aaccaggttc gccccggccc tttatctatg ccctgtcgct
     3721 cggtgtctac tgcacctcat ggacctattt cggcagtgtc ggcatcgcct cccgcaccgg
     3781 ttttgatttc ctcccgatct atatcgggcc gatcctggtt tttgccctcg gctggccgct
     3841 tctgcagcgc atcgccgaaa tcgccaaacg ccagaacatc gcctcgatcg ccgatttcct
     3901 gtcagcccgc tatggcaaga gcgaagccct tggcgccctg gtggcggtgg tggcgaccat
     3961 cggcgtggtg ccctatattt ccattcagct gaaggccgtg tcgcagtccc tgcagaccat
     4021 gatcaccgac cccctgttcc ccgaggacat catgccggtc atgcagggcg ctgacgggct
     4081 gtccatgctg gtggcgctca ccatgggcgt ctttgccatc ctgttcggca cccgccacat
     4141 tgataccacc gagcaccagg atggaatgat cctcgccatt tccgttgaat ccatcgtcaa
     4201 gcttgtggct ttcatcgcgg tggggctgtt cgtcaccctc accatgatgg gtggcttcga
     4261 aaaacttgcc gcccaagtca ccgccgatcc gcatatctat gccttgctgg ccaatggcct
     4321 gagcggcacc cgctggctga cgctcaccct tcttgccgcc atcgccataa ttcttttgcc
     4381 gcgtcagttt catgtggcgg tggtcgaaaa ctcggatgaa aagcagc
//
LOCUS       DAORVN010000068         4425 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_14656_length_4425_cov_3.769565, whole genome shotgun sequence.
ACCESSION   DAORVN010000068 DAORVN010000000
VERSION     DAORVN010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 4425)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4425)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4425
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14656_length_4425_cov_3.769565"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..816
                     /locus_tag="PKV23_02105"
     CDS             <1..816
                     /locus_tag="PKV23_02105"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HQX84255.1"
                     /translation="FLSTEQTREKLNSVRYFESISNSGAFHIHPLKYSLMLAREAEKS
                     GASLHESSPALAVEKLGNEWRVRCAEGEVIAQHVVHCVSSLDRTIHKASGRAVLPVAT
                     YVAVTEPLNQDAVRTSAAVADTRRAGNYYRLIDGGRLLWGGNITTRVSEPERLAEQMK
                     GDMLATYPQLGNPRIEYSWAGLMGYALHKMPLIGRDKDGQWFATAFGGHGMNTTAMAG
                     QLLANAIADGNDEYRRFAPFAPLWAGGQLGRAGVQASYWWMQLRDRIDEARAR"
     gene            920..1336
                     /gene="dksA"
                     /locus_tag="PKV23_02110"
     CDS             920..1336
                     /gene="dksA"
                     /locus_tag="PKV23_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010660068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HQX84256.1"
                     /translation="MNVLVDVDYRPSDDEPFMNERQAAYFRQKLNAWKEDILRESRET
                     ITHLQDENHVLPDLADRASSETDRSLELRARDRQRKLISKIEAALQRIDNGTYGYCEE
                     TGDPISLKRLDARPIATLSIEAQERHERRERVYRDD"
     gene            complement(1321..3117)
                     /gene="ade"
                     /locus_tag="PKV23_02115"
     CDS             complement(1321..3117)
                     /gene="ade"
                     /locus_tag="PKV23_02115"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018428712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HQX84257.1"
                     /translation="MKPWSDLSLTLINVAAGRAAADMVITNGRWVNVHSGEIIPGTDI
                     AIAGQRFAYVGPNARPMIGKSTKVIDAAGRYLVPGLCDGHMHVESGMVTVTEFARAVI
                     PHGTTSMFIDPHEIANVLGVAGVRLMHDEAAGLPVNVFVQMPSCVPSAPGLENAGAAI
                     TPENVADAMTWPNIIGLGEMMNFPGVINGDPQMLAEIAATQRAGKTVGGHYAAPDLGP
                     PFHAYAAGGPADDHEGTNLDDCIARVRQGMRAMLRLGSAWYDVAAQIKAVTERGIDSR
                     NIILCTDDSHSGTLVYEGHMNRVVRHAIAEGVNPVTAIQMATLNTAQHFGLERELGSI
                     TPGRRADLILSSDLTTLPIELVIAHGQVMAENGKLVVELPKFTYPEFARNTVHVGRTL
                     GAKDFEIAAPKGANKVEARVIGVIENQAPTKALQRPLVVKSGLVEMDHANDVCQIAVV
                     ERHRGLGTVVNAFVSGFGYNVECAVASTVAHDSHHIIVVGTNHDDMALAANTLSEIGG
                     GVVVVSNGRELARVELVVAGLMSDEPAEIVAAKAEKMIAAMKACGCNLNNAFMQHSLL
                     ALVVIPELRISDMGLIDVRTFEKVDLFVQSSR"
     gene            complement(3114..3896)
                     /locus_tag="PKV23_02120"
     CDS             complement(3114..3896)
                     /locus_tag="PKV23_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012110892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HQX84258.1"
                     /translation="MDWTELTNFAVSLARASEAEILPHFRRNAAVDAKPHHSWDPVTE
                     GDRAGERIIRKLIEQRFPEHGINGEEYGIKEGKSGLTWVLDPIDGTRSFVCGMPTWST
                     LIALYAEGKPVIGVMNQPFVGDLFYGNPHGAWNTYRGATEPLRTRQGVGISQAAAGTT
                     APELYRGKTDMEAFQKLQNAVRLMRFGGDAYFYSVLAAGHLDIAMDAGLQAYDIGALI
                     PIIESAGGAVGSWDGGNPAEGGNVICAGSRPLLEAAIAVMTS"
BASE COUNT          920 a         1234 c         1351 g          920 t
ORIGIN      
        1 tttctttcga cggaacagac tcgagagaaa ctgaattcag tccgctattt tgagagcatt
       61 tccaactccg gcgcgtttca catccacccc ctgaaatact cgctgatgct ggctcgcgag
      121 gcagaaaaga gcggggccag cttgcatgaa agctcgccgg cgcttgcggt ggagaagctg
      181 ggtaatgaat ggcgggtgcg ctgcgctgaa ggcgaagtta tcgctcagca tgtggtgcat
      241 tgcgtatctt cgctggaccg caccatacac aaagccagcg gccgggcggt tctgcccgta
      301 gcaacctatg tggctgtgac tgaaccgttg aaccaggacg cggtgcgaac cagcgctgcg
      361 gtggcagata cgcggagagc aggcaattat taccggctca ttgacggggg caggctgctg
      421 tggggcggga atatcaccac acgggtttcg gagcccgagc gccttgccga gcaaatgaaa
      481 ggcgatatgc tggcaaccta tccgcaactg gggaatccgc gcatcgagta ttcctgggcg
      541 ggcctcatgg gctatgccct gcacaagatg ccactcatcg ggcgggacaa agatggacaa
      601 tggtttgcca cggcctttgg cgggcatggc atgaacacca cagcgatggc gggacaattg
      661 ctggcgaatg caattgccga tggaaacgac gaataccggc ggtttgcgcc ttttgccccg
      721 ctttgggcgg ggggccagct ggggcgggcc ggagtgcagg cgtcgtactg gtggatgcag
      781 ctccgtgacc gaatcgacga ggcgagggcg cgctagaacg gcattgtttt tgcagggttt
      841 tgggcctgaa tcacgggcta ttgtcgaagc agtccaaggc cgctataagc ccgcgccacg
      901 gtagctggag tgacgattta tgaatgtact tgtcgatgtt gactacagac ccagtgacga
      961 cgagcctttc atgaacgaac ggcaggcggc atatttcagg caaaagctga atgcctggaa
     1021 ggaagacatc ctgcgtgaaa gcagggaaac catcacccat ttgcaggacg agaaccatgt
     1081 cctgccggat ctggcggacc gcgcctcgtc ggaaaccgac cgttcccttg agcttcgggc
     1141 ccgcgaccgg cagcgcaagc tgatttcaaa gattgaagca gccctgcagc gcatcgacaa
     1201 tggcacctac ggctactgcg aagagacggg cgacccgatc agcctgaagc gccttgatgc
     1261 gcgccccata gcgacgctct ccatcgaagc gcaggaacgc catgaacggc gcgagcgggt
     1321 ttaccgcgac gactgaacaa acagatcgac cttttcgaaa gtcctgacat cgatcagccc
     1381 catgtcggaa atgcgcagtt cggggatgac gacaagcgcc agcagggaat gctgcatgaa
     1441 ggcgttgttc aggttgcagc cgcaagcctt catggctgct atcatttttt cggcttttgc
     1501 cgcgacgatt tcggcaggtt cgtcggacat gaggccggct accaccagct cgacgcgcgc
     1561 caattccctg ccgttgctta cgacaaccac gccgccaccg atttcactca gggtgtttgc
     1621 ggccagggcc atatcatcgt gattggtgcc gacaacgatg atgtgatggc tgtcatgggc
     1681 cacggttgag gccacggcac actcaacatt gtagccaaag cccgacacaa aggcgttgac
     1741 cacggtgccc agcccgcgat gtcgttcgac tacggcgatc tggcagacat cgttggcgtg
     1801 gtccatttcg accaagccag atttcactac gagcggccgc tgcaacgctt tggtcggggc
     1861 ctggttttca atgactccaa tgacgcgggc ttcgacctta ttggcgcctt tgggagcggc
     1921 gatctcaaaa tcctttgccc ccagcgttcg gccgacatgc acggtgttcc tggcaaactc
     1981 gggataggta aacttcggca gctccacgac gagcttgcca ttctcggcca tcacctggcc
     2041 atgggcgatg accagttcaa tgggcaaagt ggtgagatcg gacgagagaa tgaggtcggc
     2101 ccggcggccc ggcgtgatgg agcccagttc gcgctcaagc ccgaaatgct gggcggtgtt
     2161 cagggttgcc atttgtatgg cggtgaccgg attcacgccc tctgcaatag catggcgcac
     2221 gacgcgattc atgtggccct cgtagacgag cgtccccgaa tggctgtcat ccgtacacag
     2281 aatgatatta cgggagtcaa tgccgcgctc ggtcacggcc ttgatctggg ccgccacgtc
     2341 ataccaggcc gagccgaggc gcagcattgc gcgcatgccc tggcgcaccc gggcaatgca
     2401 gtcatcgagg ttggtgcctt catggtcatc ggcgggaccg ccggctgcgt aggcatggaa
     2461 gggagggcca aggtccggcg cggcatagtg gccgccaact gtttttccgg ctctctgggt
     2521 ggcggcaatc tcggccagca tttgtggatc accattgatc accccgggga agttcatcat
     2581 ttcgccgagc ccgatgatat tgggccaggt catggcgtca gccacgtttt cgggggtaat
     2641 ggcggcgcca gcgttttcaa ggccgggggc gctgggaaca caactcggca tctgcacaaa
     2701 gacattgacg ggcaggcccg cggcttcatc gtgcatcagc cgcacacccg ccacgcccag
     2761 gacattggcg atctcatggg gatcgatgaa catggaggtg gtgccgtgcg ggatgacggc
     2821 gcgggcaaat tcagtgacag tcaccatgcc gctttcaaca tgcatgtgac catcgcaaag
     2881 ccccggcacg agatagcggc cggcggcatc gatgactttg gttgatttgc caatcatcgg
     2941 cctggcatta ggccccacat aggcaaagcg ctggccagcg atggcgatat cggtgccggg
     3001 gatgatttct ccggagtgga cattcaccca gcggccattg gtaatcacca tgtcggcagc
     3061 ggcccggcct gctgccacgt taatcagagt gaggctaagg tcggaccagg gtttcatgag
     3121 gtcattactg ctatggcagc ttcaagcaga ggcctggagc ccgcgcagat gacattaccg
     3181 ccttcggccg gatttccccc atcccagctg ccaaccgcgc cgcctgcgct ttcaatgatg
     3241 ggaatgaggg cgccgatatc ataggcctgg aggccagcat ccatggcgat gtcgaggtga
     3301 cccgcagcca ggacggaata gaaataggca tcccctccaa agcgcatgag cctcacggca
     3361 ttttgcagct tctggaaagc ttccatgtcg gttttgcccc ggtaaagttc gggagctgtt
     3421 gtgccggccg ccgcctggct gatgcctacg ccctggcggg tacggagggg ttcagtggca
     3481 ccgcgatagg tgttccaggc gccatgggga ttgccgtaga aaaggtcgcc cacgaagggc
     3541 tggttcatga cgccaatcac cggttttccc tctgcataga gggcgatgag ggtggaccag
     3601 gtcggcatgc cgcagacaaa agaccgggtg ccgtcgatgg gatcgagaac ccaggtcagg
     3661 ccggatttgc cttccttgat gccatattcc tcgccattga tgccgtgctc gggaaagcgc
     3721 tgctcgatca gtttgcgaat gatccgctcg cccgcccggt caccttcggt aaccgggtcc
     3781 caggagtgat ggggtttcgc gtcgacggcc gcgttacggc ggaagtgggg gagaatttca
     3841 gcttccgaag cccgcgccag acttacggcg aaatttgtca gctcagtcca gtccataaac
     3901 cccatttccc tttgacccag tcctatagga gagccgggtc cggctcaagg tgggggaaaa
     3961 cccatgctat tccgcactgc acaatgagat gtatttgcca tgcaaacggt gcatggcctg
     4021 tccgcccatg cgttctaaaa tcatacaaat agtataaaat caatttatat cagttactta
     4081 actgttttta acgcgtcggg ggagtgttgc aaatttgatc acttggaaag ttatttaggc
     4141 aatggtcttg aattgaaaat gttgcggtgc catatcggag aggcaagacc aaggcatctg
     4201 ccttctcttg cagcccttct tgggcgtttc ctccctagac ttgggccggt cactttagtg
     4261 tatcggccct ttttttattc cgccgcctgt ttctgcggat ttgcgaactc caccagggtt
     4321 tcaagcagga ttttaatggt atttgcgagg gtccgctgca agggcagaaa atccgcggtt
     4381 ttttcgaagc gcaattcatc caggtaaagg ccgcggttta tttcg
//
LOCUS       DAORVN010000069         4414 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_14725_length_4414_cov_3.487497, whole genome shotgun sequence.
ACCESSION   DAORVN010000069 DAORVN010000000
VERSION     DAORVN010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 4414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4414
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14725_length_4414_cov_3.487497"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1192)
                     /locus_tag="PKV23_02125"
     CDS             complement(<1..1192)
                     /locus_tag="PKV23_02125"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006204113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HQX84259.1"
                     /translation="MPSFKNSDVRLPIMEGSPPKLVPPRLDWDRPPWNRWSFQHVREI
                     LPTAEIWRGYGPVRALPRADADLDNLSVADAAGTSIPLMGFLDETYTDGFLVLKNGGI
                     SYERYFNGMDHRTLHLSQSMSKSFVGMTAGILASRGLLDVNAPVTAYLPELEQTGWRG
                     ATLQQVLDMTTGTRFSEDYVNPYSDVGQMDVASDWKPVPPDSDASFKWPRHMWELITG
                     LTETVRGHGDLFAYRSVETDVLAFCLERVTGKRLPQLLSEEIWQKMGAEESSCITVDS
                     AGYALASGGLNATLRDYGRFGQLVLENGGGIIPAPWIEATRSGDHSKFKEPYTFSIPQ
                     GAYKNQFWIEDPSSRAILAKGVFGQLIYINWDYNMVVVKLSSWPDFLNRPFDVATFCA
                     IHAIA"
     gene            complement(1253..2287)
                     /locus_tag="PKV23_02130"
     CDS             complement(1253..2287)
                     /locus_tag="PKV23_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010911253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HQX84260.1"
                     /translation="MNWKLTAALAGAALFAAAGTAHAEGELNIYNWGNYTSPEMIKKF
                     EEAYKVKVTVTDFDANDTALAKVRAGGHGFDIVVPSANYVPIWVKEGLLLESRPDQME
                     NFKHVDDRWVNVDWDPGRHYSVPWQWGLIGVVVDTSVYKGDINTSAIWLDPPPELVGK
                     INVAPEMNDVMYAAIRYMGGQWCSDDKEVLKKVRDKLVEAKPKWIAMDYGTIEKFASR
                     DYGAAFYWNGAAMRSREQNPDVRFGYPKEGFAYFMDSAAILKDAKNPDNAKLFLNFVM
                     DPENAAMISNFAKYANGIKGSDAFMDPAMKDAPELNVPAEFQAAGEFLKSCAPEVTDL
                     YTRIWTELLK"
     gene            complement(2316..3116)
                     /locus_tag="PKV23_02135"
     CDS             complement(2316..3116)
                     /locus_tag="PKV23_02135"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019860924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQX84261.1"
                     /translation="MARTFSVRTQPGFTFIAMLCFFLLYLPIATLVVYAFNSGQSVAV
                     WEGFSFRWFESAWNNRAIQEASLRSIFIATFAATVATLVATMAALATTRTAPYPGLTF
                     KYAFINQPLMVPEIVTAVALLIVFSRIKVWTGYSGIAYLLAAHTAFCIPFAYLPIRAR
                     LENMDLTLERAAADLYATPWNAFKRVTLPLLWPGIIAGFMLAFVISLDDVVITEFVKS
                     GGQETLPTYMLGQLRRIVTPEINAIATVFLLISVAFVTMFFIINRKKP"
     gene            complement(3109..4032)
                     /locus_tag="PKV23_02140"
     CDS             complement(3109..4032)
                     /locus_tag="PKV23_02140"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010911255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQX84262.1"
                     /translation="MATPREIAVAAERQDIRNRWLLSSPALVIIALAAIGPLFVMLLY
                     SFMLKGDYGDVKAWQFSSDGWFGVFLQRDIFDDTLTLADAHVTIFWRSVKLSLMTTVV
                     TLFLGFPTAYFIATRPAKQRDIWLFLVTIPFWTNILIRTFAMQEVIRNEGIVNSAMIW
                     IGIIDAPIQIMYTDWAILFGMAYVYLPLMVLPLYASMEKIDFRLVEAGYDLYATRLQV
                     LRRIIIPLVKPGIIAGSILVFIPSLGAYVTPRVLGGGKNMMLGNLIELQFGQGRNWPL
                     GAALSITLMIIVTLALLVYVKNASKSGGGHG"
     gene            complement(4032..>4414)
                     /locus_tag="PKV23_02145"
     CDS             complement(4032..>4414)
                     /locus_tag="PKV23_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006204109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HQX84263.1"
                     /translation="ANFIGETNFLEGKVVSTAKGQAKIALDAGTTITASLGDGAKPSG
                     KVTVVVRPEHASLVAASGKATLSGKLENIVYFGTDTHYHVKLHGGSEFTVRHQNVHAG
                     ALAFEKGDKVGVLFGADAARVLRD"
BASE COUNT          955 a         1300 c         1273 g          886 t
ORIGIN      
        1 cggcaatcgc gtggatggcg cagaatgtgg cgacgtcaaa cgggcggttg agaaaatccg
       61 gccagctcga aagcttgacc accaccatgt tgtagtccca gttgatatag atcagctgcc
      121 cgaagacgcc ctttgccaga atggcccgcg aggatggatc ctcgatccag aactggttct
      181 tgtaagcccc ttgcggaatt gaaaatgtat agggctcctt gaatttgctg tgatcgccgc
      241 tgcgcgtcgc ttcaatccac ggcgccggaa tgatgccgcc gccattttca aggacaagct
      301 ggccaaaccg gccatagtcc cgcaaggtgg cgttcaggcc gcctgatgca agggcatacc
      361 cggcgctgtc gaccgtgatg cagctgctct cttccgcccc catcttctgc cagatttcct
      421 cggacagaag ctgtggcagg cgcttgcccg tgacgcgctc caggcaaaac gccagaacat
      481 cggtttcaac cgagcggtag gcaaacaggt cgccatgtcc ccgcacggtc tcggtcaacc
      541 ccgtgatcag ttcccacata tggcgcggcc atttgaaact ggcgtcgctg tccggcggaa
      601 ccggcttcca gtcgctggcc acgtccatct ggcccacgtc cgaataggga ttgacgtaat
      661 cctcggaaaa acgcgtgccc gttgtcatgt cgagtacctg ctgcagagtt gctccgcgcc
      721 agcccgtttg ttcaagctct ggaagatagg ccgtaaccgg cgcattcacg tcaagcagcc
      781 cgcggctggc gagaatgccg gcagtcatgc cgacaaagga tttcgacatc gattgcgaga
      841 gatgcagcgt gcggtggtcc atgccgttga aataacgctc ataggaaatg ccgccattct
      901 ttaaaaccag aaagccgtcc gtataggtct cgtcaagaaa ccccatcagc gggatcgaag
      961 tgcccgcggc atcggcaacc gaaaggttat ccagatcggc atcggcgcgc ggcaaggcgc
     1021 gcaccggccc gtatccgcgc cagatttccg cagtgggcag gatttcccga acgtgctgga
     1081 acgaccaccg gttccacggc ggccggtccc agtccagtct cggcggaacc agtttgggcg
     1141 gcgagccttc catgatcggc aggcgcacgt cggaattctt gaatgatggc atggcgtctc
     1201 ccggacaaca aaaaagcccc ggcagtttcc cgccggggca ttaaatgcaa gcttacttca
     1261 gaagttccgt ccagatcctt gtgtaaaggt ccgtaacctc cggcgcgcag gacttgagga
     1321 attcacccgc tgcctggaat tccgccggaa cgttaagttc gggcgcatcc ttcatggccg
     1381 gatccatgaa cgcatccgat cccttgatgc catttgcgta tttggcaaag ttggagatca
     1441 tcgcggcatt ttccggatcc atgacgaagt tcaggaacag cttggcattg tcgggattct
     1501 ttgcgtcctt gagaatggcc gcgctatcca tgaaataagc gaagccctcc ttcggatagc
     1561 caaagcgcac atccgggttt tgctcgcgcg agcgcatggc ggcgccattc caataaaagg
     1621 ccgcaccata atcacggctg gcaaattttt cgatggtgcc gtagtccatg gcgatccact
     1681 tcggcttcgc ctcaacaagc ttgtcacgca ccttctttag gacttctttg tcatccgagc
     1741 accattgccc gcccatgtag cggatcgcgg catacatcac gtcgttcatt tccggtgcca
     1801 cgttgatctt gcccaccagt tctggcggcg ggtcaagcca gatggccgag gtgttgatgt
     1861 cccctttgta gaccgacgta tccactacaa cgcctatcaa tccccattgc catggaaccg
     1921 agtaatgccg gcccggatcc cagtcgacgt tcacccagcg gtcatcgaca tgcttgaaat
     1981 tctccatctg gtcggggcgc gattccagca gtaggccttc cttgacccag atcggcacgt
     2041 aatttgccga aggaaccacg atgtcaaagc cgtggccgcc ggcgcggacc ttggccagcg
     2101 ccgtgtcgtt tgcgtcaaaa tcggtgaccg tgaccttgac cttgtaggct tcctcgaatt
     2161 tcttgatcat ttcagggctg gtgtagttgc cccagttgta aatgttgagc tcgccttcgg
     2221 catgggcagt gcccgccgcg gcgaaaagtg cggcacctgc gagcgccgct gtcagtttcc
     2281 agttcatggt tgtctttctc cttgttgtac gagcatcatg gtttcttgcg gttgataatg
     2341 aagaacattg tcacgaatgc gaccgaaatc agcaggaaca ccgtcgcgat cgcgttgatc
     2401 tcgggtgtca cgatgcggcg caattgcccc agcatgtagg tcggcagggt ctcctgcccg
     2461 cccgatttca cgaactccgt tattaccaca tcgtcaagcg aaatcacgaa ggccagcatg
     2521 aacccggcta tgatgccagg ccacaaaagc ggcaaagtaa cgcgtttgaa ggcgttccac
     2581 ggggtggcgt agaggtcggc ggcggcccgt tccagcgtca ggtccatgtt ttccagccgc
     2641 gcccggatcg gcaggtaggc aaaggggatg cagaacgccg tatgggcggc cagcagatag
     2701 gcaatgccgg aatacccggt ccacaccttg atgcgcgaga aaacgatgag cagggcaacg
     2761 gccgtgacga tttcgggcac catcagcggc tggttgatga aggcgtattt gaaggtgagg
     2821 ccgggatagg gcgccgtccg cgtggtggct agcgccgcca ttgtggccac cagtgtcgca
     2881 acggttgccg cgaacgtggc gatgaagatc gagcgcaatg aggcttcctg gatggcccgg
     2941 ttgttccatg ccgactcgaa ccagcggaat gaaaagccct cccagaccgc aaccgactgc
     3001 cccgaattga aggcgtaaac caccagcgtc gcgatcggca ggtagagcag gaagaaacac
     3061 agcattgcga tgaaggtgaa gcccggctgg gtcctcacgg aaaatgttct agccatggcc
     3121 gcctccggat ttggaggcgt ttttcacgta gacgagcagg gcgagcgtca cgatgatcat
     3181 cagcgtgatc gacagcgccg cgcccagcgg ccagttgcgc ccctggccga actgcagctc
     3241 gatgagattg ccgagcatca tgtttttgcc gccgcccagc acccgcggcg ttacataggc
     3301 gccaagcgaa gggatgaaca ccaggatgga tcctgcaatg atgccgggct tcaccagcgg
     3361 aatgatgatg cggcgcagta cctggagccg cgtcgcataa agatcatagc ccgcttccac
     3421 cagcctgaaa tcaatcttct ccatgctggc gtaaaggggc agcaccatca gcggcaggta
     3481 gacataggcc atgccgaaca ggatggccca gtccgtgtac atgatctgga taggcgcatc
     3541 gatgatcccg atccagatca tcgcgctatt gacgatgcct tcgttgcgga tcacctcctg
     3601 catggcgaaa gtccggatca ggatgttggt ccagaacgga atggtcacca ggaaaagcca
     3661 gatgtcgcgc tgcttggccg ggcgcgttgc aatgaaataa gcggtcggga atccgagaaa
     3721 aagcgtgacc actgtcgtca tcagcgacag cttgaccgag cgccagaaaa tcgtgacgtg
     3781 ggcatcggcc agagtcagcg tgtcatcgaa gatatcgcgc tgcagaaaaa cgccaaacca
     3841 gccatcgctc gaaaactgcc aggcctttac gtcgccgtag tcacccttga gcatgaagga
     3901 gtagagcagc atcacgaaca aagggccgat tgccgcaagt gcgatgatca ccagggcggg
     3961 agaagacaag agccacctgt tgcggatgtc ctgccgttcc gccgccacgg cgatttcgcg
     4021 cggcgttgcc atcaatccct cagcacacgg gctgcatcgg cgccaaacag cacgcccacc
     4081 ttgtcgccct tttcaaaggc cagggcgccg gcgtggacat tctggtggcg gaccgtgaac
     4141 tcgctgccgc catggagctt cacatggtag tgcgtgtccg ttccgaaata gacaatattt
     4201 tcgagttttc ccgaaagtgt cgccttgcct gatgccgcaa caaggcttgc atgttcggga
     4261 cgcaccacaa ccgtcacttt gcccgatggc ttggcgccgt cgccaagcga agcagtgatc
     4321 gtggtacccg catcaagcgc aatcttggcc tggcccttgg cggttgaaac aaccttgccc
     4381 tcgaggaaat tcgtttcgcc aatgaaattc gcca
//
LOCUS       DAORVN010000070         4396 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_14834_length_4396_cov_3.092836, whole genome shotgun sequence.
ACCESSION   DAORVN010000070 DAORVN010000000
VERSION     DAORVN010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 4396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4396
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14834_length_4396_cov_3.092836"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..446)
                     /locus_tag="PKV23_02150"
     CDS             complement(<1..446)
                     /locus_tag="PKV23_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010968505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imelysin family protein"
                     /protein_id="HQX84264.1"
                     /translation="MKRIPKLWTGLGVAAIAGSMAAQADVISLQKGASQSPVILAQEG
                     GEGGGEGGGGAPTTYALESTDSNAFKYDATPQIDAYIDLVASAYKEAADDARTLAAAV
                     DALLASPSGDTLKAARKAWVEARPAYLKTETFRFYDHISAHTMRRTA"
     gene            698..1732
                     /locus_tag="PKV23_02155"
     CDS             698..1732
                     /locus_tag="PKV23_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018454614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HQX84265.1"
                     /translation="MADINETTGLEPPRVYLVHMVVFTLVVVVLAAVLYPSIKAAFMA
                     NVGLNGLIIGLLILGVLYAYRMVWRLFPEINWVNGFRLGQSSDENLAEPVLLAPMATL
                     LGDRQERTVLSTNTMRSLLDSLASRLDESRDLMRYMVSLLIFLGLLGTFWGLLETVTS
                     VGDAIKNLDVGAGQSSSMFEELKTGLQRPLEGMGLSFSASLFGLAGSLILGFLDLKAS
                     QAQNRFYHNLEDWLSTITDIQVGEGGSQPGATNYLRFDMQGLQRGLDKVGKQLEGVKV
                     NGAGGGNANESIEQLAEAVASLVQQMREEQKMVRQWAQSQQVQQNEIQRLLIRSTGQP
                     AGRARSKSDE"
     gene            1738..2769
                     /locus_tag="PKV23_02160"
     CDS             1738..2769
                     /locus_tag="PKV23_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008188981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan -binding protein"
                     /protein_id="HQX84266.1"
                     /translation="MAGALSKRRRAEDAPYWPGFVDAMAQLLLVITFLLSVFMTAQFL
                     LAREMSGQDSALGQLRSQIAELTQLLALEKSAKGAVVANLLALSDDLSAERQKNAGLL
                     ANLAAEGSKGSAANQTISGLQTALDKEKALTSEALSQVELLNQQIAAMRRQLAQLNSI
                     LTDAETRNKSSEAQIADLGKRLNSALAQRVQELSRYRSEFFGRLRQILSQRSDILVVG
                     DRFVFQSEVLFAKGDAEISPLGQEEMLKLADALRQLEAEIPQDIAWVLRVDGHTDIDP
                     IKSPFFQSNWELSSARAIAVVKFLIAQGIAPKHLVAAGFGEFQPLDVGTSEDAKSQNR
                     RIELKLTER"
     gene            complement(2750..>4396)
                     /locus_tag="PKV23_02165"
     CDS             complement(2750..>4396)
                     /locus_tag="PKV23_02165"
                     /inference="COORDINATES: protein motif:HMM:TIGR02204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HQX84267.1"
                     /translation="TLVVPIAVRRIIDHGFTASNATLVNQYFSIMIAVVLLLALSSAI
                     RFYYVMWLGEKIVADVRDALFTHLLKLSPGFYETQKTGEVVSRLTADTTQIKAAFSST
                     ASIALRNGMMLMGAIVMMIVTSPKLAGLAGLAIPLIVLPLVTYGRRVRLLSRAAQDTL
                     AASAAFAQERLSAITTVQSNVQEAFTEGEFARATHFAFSAAARRTFARAVLTAAIIFV
                     ALGSIVGLLWFGAQEVISGNLSGGTLSQFVIYAVIAASSLGQLSEVWGEVQLAAGAAE
                     RISELLDEKPLITAPPHPAVFPKSPKGQVKFSGVTFHYPTRLKSTAISNVSFVAKPGE
                     FTAVVGPSGAGKSTLFALIQRYYDPASGTIAIDGLDIRTLDPQELRKHIAVVPQDTIV
                     FSGTVLDNIRFGAPDATRETAMAAAVAARVDEFAEKLPKGFDTEVGERGVTLSGGQRQ
                     RIAIARAILRDAPILLLDEATSALDAESESLIQDALEKLTANRTTLVVAHRLATVRNA
                     DRILVFDNGKLVAQGTHGELVKKNALYARLAKLQFSAPSA"
BASE COUNT          855 a         1222 c         1492 g          827 t
ORIGIN      
        1 gcagtacgac gcatggtatg tgccgatata tgatcataga aacggaaggt ttcggttttc
       61 agataggcgg ggcgggcctc gacccaggcc ttgcgggcgg ccttcagggt gtcgccggaa
      121 ggagaggcga gcaacgcgtc gacggccgcg gcaagagtcc tggcatcatc ggcggcttcc
      181 ttgtaggcgc tggccacgag atcgatatag gcatcgatct ggggtgtggc gtcgtatttg
      241 aaagcgttcg aatcggtcga ttcgagggca taggtggtgg gcgctccacc gccaccttcg
      301 ccgccgcctt caccgccttc ctgggcgagg atgacgggtg actggctggc gcctttctgc
      361 agcgagatga cgtcggcctg ggccgccatt gaaccggcaa ttgccgcaac gccaaggccc
      421 gtccacaatt tgggtatccg cttcatcgca ctcatcctgc ttttgctgtt gtgattgatg
      481 gctgagtagc atgcgggacg gtctcataca atagttgatt aaaatactcg gaattagaat
      541 tgttccaaaa taggagagat ttggcgggcc caaaccagcc atttttgcgt cggcgattct
      601 ctcatgttaa caacggaagt tgcgcctcgc ccgcggtcac gcaatcgtct agatttgctg
      661 gaagtgtcgg ggcggacggg aggcggttga ggattcaatg gcagatataa acgaaacgac
      721 agggctggag ccgccgcgcg tctatcttgt ccacatggtt gtgttcacac tggtcgtcgt
      781 tgtcctggcc gccgttctct accccagcat caaggcggcc ttcatggcca atgtgggtct
      841 caatgggctg atcattggcc tgctcatact cggggttttg tatgcctacc gcatggtctg
      901 gcggctgttt ccggaaatca actgggtcaa cggcttccgg ctcggccaat cgagcgacga
      961 gaacctggcg gagccggttc tgctcgcccc catggccacc cttctgggcg accggcagga
     1021 acgcacggtt ctttccacca acaccatgcg atcactgctc gattcgctgg cctcgcggct
     1081 ggacgaatcg cgcgacctga tgcgctacat ggtcagcctg ctgattttcc tggggctgct
     1141 cggaacgttc tggggcctgc tcgagaccgt gacctcggtg ggcgatgcca tcaagaacct
     1201 ggatgtgggg gcggggcaat cgagctccat gttcgaggaa ctgaaaaccg gcctgcagcg
     1261 gccgctcgaa ggcatggggc tttcgttttc cgccagcctg ttcgggcttg cgggttccct
     1321 gattcttggc ttcctggatc tcaaggcaag ccaggcgcag aaccgcttct accacaatct
     1381 ggaagactgg ctttcaacca tcaccgacat ccaggtgggc gagggaggca gccagcccgg
     1441 ggccaccaat tacctgcgct ttgacatgca gggactgcag cgcggcctgg acaaggtggg
     1501 caagcagctt gaaggcgtga aggtcaacgg ggccggcggc ggcaacgcca atgaatcgat
     1561 cgaacagctg gccgaagcgg tggccagcct ggtgcagcag atgcgcgagg agcagaaaat
     1621 ggtgcggcag tgggcgcaga gccagcaggt gcagcagaat gaaatccagc gcctgctcat
     1681 ccgttcgacg ggccagccgg ccgggcgggc ccgcagcaag agcgacgagt aacggccatg
     1741 gcgggcgccc tttcaaaacg cagacgggcg gaagacgcgc cctactggcc aggcttcgtg
     1801 gacgccatgg cccagctcct gctggtcatc accttcctgc tctcggtgtt catgacggcg
     1861 cagttccttc tggcgcgcga aatgtcgggt caggattcag cccttggaca attgcgctcg
     1921 cagattgccg agctcacgca gctgctggcg cttgaaaaat ccgccaaggg cgcggtggta
     1981 gcaaaccttt tggcgctgag tgatgacttg agcgccgagc ggcagaaaaa tgcgggcctt
     2041 ctggccaacc tggcggcgga gggttcaaag ggctcggcgg caaaccagac gatcagcggc
     2101 ttgcaaaccg ccctcgacaa ggaaaaggcg ctcacgtcgg aggcgctctc ccaggtggaa
     2161 ctgctgaacc agcagattgc cgccatgcgc cggcaactgg cgcagctcaa cagcatcctg
     2221 accgacgcgg agacccgcaa caagtcctcg gaggcgcaga ttgccgacct tggcaaacgg
     2281 ctgaattcag ccctggcgca gcgggtgcag gaactgtcac gctaccggtc ggaattcttc
     2341 gggcggctca ggcagattct ttcgcagcgc tcggatatcc tggtggtggg cgaccgtttc
     2401 gtcttccagt cggaagttct gttcgccaag ggcgacgcgg aaataagccc gctgggacag
     2461 gaggaaatgc tgaagctggc ggatgcgctg cggcagcttg aagcggaaat tccccaggat
     2521 atcgcctggg tcctccgggt tgacgggcac acggacatcg atccgatcaa gtcacccttc
     2581 tttcaatcga actgggagct ctcctcggcg cgggccattg cggtggtcaa attcctgatt
     2641 gcccagggca ttgcgccaaa gcatctggtg gcggcgggct tcggcgaatt ccagccgctc
     2701 gatgtgggca cctcggaaga tgccaaatcg cagaaccggc gcatcgagct caagctgacg
     2761 gagcgctgaa ctgcagtttt gccaggcggg cgtagagggc gttcttcttg acgagttcgc
     2821 cgtgggtgcc ctgggccacg agcttgccat tgtcgaaaac aaggatgcgg tcggcattgc
     2881 gcacggttgc caggcgatgg gcgacaacga gggtggtgcg gttggcggtg agcttttcca
     2941 gcgcatcctg gatgagggac tcgctttccg catcaagggc gctggttgct tcatcgagca
     3001 gcaggatggg ggcatcgcgc agaatggcgc gggcgatggc gatgcgctgg cgctggccgc
     3061 cggacagggt gacgccgcgc tcgcccactt cagtgtcaaa gcccttggga agcttttcgg
     3121 caaactcgtc aacacgggcc gcaacagcgg cggccatggc ggtttcgcgg gtcgcgtcgg
     3181 gcgcgccgaa gcggatgttg tcgaggaccg tgcctgaaaa aacgatggtg tcctggggca
     3241 cgacggcgat gtgcttgcgc agttcctgcg gatccagcgt cctgatgtcg agaccatcga
     3301 tggcgatggt tccggaggcg gggtcgtaat agcgctggat gagggcgaag agggttgact
     3361 tgccggcccc cgacgggccc acaacggcgg tgaattcgcc gggcttagcc acgaagctca
     3421 cgttgcttat cgccgttgac ttcagccggg tgggataatg gaaggtgacg ccggaaaact
     3481 tgacttgtcc cttcggcgat tttggaaaca ccgcgggatg gggcggggcg gtgatcaggg
     3541 gcttttcatc gagcagttcg gagatgcgtt cggcggcgcc ggcggcgagc tggacttcgc
     3601 cccagacctc ggacaactgg cccagcgaac ttgcggcgat gacggcgtag atgacgaact
     3661 ggctcagggt gccgccagac agatttccgc tgatcacttc ctgggcgccg aaccagagca
     3721 ggccgacgat cgaacccagg gccacgaaga tgatggctgc ggtgagaacg gcgcgggcga
     3781 aggtgcgcct ggcggcggcg gaaaaggcga aatgcgttgc cctggcgaac tcgccttcag
     3841 tgaaggcctc ctggacattg gattgcaccg tagtgatggc cgaaaggcgc tcctgggcga
     3901 aggcggcgga ggcggcgagc gtatcttggg cggcgcgcga caagagccgc acccggcggc
     3961 cgtaggtgac gaggggcagg acaatgagcg gaatggcgag gcctgcgagc cctgcgagct
     4021 tggggctggt gacaatcatc atgacaatgg cgcccatgag catcatgcca ttgcggagcg
     4081 ctatcgaggc cgtggaggaa aaggccgcct tgatctgggt agtgtcggcg gtgaggcggg
     4141 agacgacctc gccggttttc tgggtttcgt agaagccggg gctgagtttc agcagatggg
     4201 tgaacagggc atcacggacg tcagcgacga ttttttcgcc gagccacatg acataataga
     4261 agcggatggc cgaactcagg gcgagcaaca ggacaacggc gatcatgatg ctgaaatact
     4321 ggttaaccag cgtggcattg gatgccgtaa agccatggtc gataatgcgg cggacggcta
     4381 tgggcacaac aagggt
//
LOCUS       DAORVN010000071         4396 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_14835_length_4396_cov_2.989864, whole genome shotgun sequence.
ACCESSION   DAORVN010000071 DAORVN010000000
VERSION     DAORVN010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 4396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4396
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14835_length_4396_cov_2.989864"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..390)
                     /locus_tag="PKV23_02170"
     CDS             complement(<1..390)
                     /locus_tag="PKV23_02170"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQX84268.1"
                     /translation="MPVAALASQNLHPEQKFSKHADGSRPRVLYAEDSTALRIVTTAM
                     LKRMGLEVDAVEDGELALRQAEKSFYDVILLDIEMPVMDGVTAARGIRALGGKTSKTP
                     ILALSAFLADSTEFSHWRDAFDHAVPKP"
     gene            complement(513..1160)
                     /gene="upp"
                     /locus_tag="PKV23_02175"
     CDS             complement(513..1160)
                     /gene="upp"
                     /locus_tag="PKV23_02175"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018184759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HQX84269.1"
                     /translation="MATSPLAKTLTIVDHPLVLHKLTLMRDKHTPSAVFRQLLREISL
                     LLAYEVSRDLPMTTQTIETPVAEMEAPILKGKKLVIVSILRAGNGLLEGMLDLMPSAR
                     VGHIGIYRNPETLQPVEYYMKVPADIEERRVIVVDPMLATGNSVAAAITRLKEKGAKD
                     IRLVCLLSAPEGIKRFSEEHPDVQIYTAAVDSHLNSHGYIVPGLGDAGDRMFGTK"
     gene            1306..1800
                     /locus_tag="PKV23_02180"
     CDS             1306..1800
                     /locus_tag="PKV23_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018316832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HQX84270.1"
                     /translation="MTQPTKPIAAGTSIGHVHLKVANIQRSLDFYCGVLGFELQQFYG
                     DQAAFVSAGGYHHHLGMNTWESLGGSPPPPGTTGLFHTAILYPTRADLGDALRRLKAH
                     NIPLDGASDHGVSEALYLRDPDQNGVELYWDRPKDKWPRKADGSLAMMTARLDLTSLL
                     AEGA"
     gene            complement(1797..2891)
                     /locus_tag="PKV23_02185"
     CDS             complement(1797..2891)
                     /locus_tag="PKV23_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013635783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HQX84271.1"
                     /translation="MTSFTPLIDSLPATVPFVGPEAILRRSGIPMRARIGANENVFGP
                     SPKAIAAMQEAASESWKYGDPEVHDLRAAIARHHGIGTENVTVGEGIDGLLGIAVRLF
                     VEPGVTVASSLGAYPTCNFHVAGFGGRLVTAPYVNDREDPSSLLDLARKENARLIYFA
                     NPDNPMGTTWKAGEVQDMIDNIPAGGVLMLDEAYHEFAEGTVPALDMANPHVLRFRTF
                     SKAYGMAGVRVGYALGHPHLISAFDKIRNHFGVNRLGQAGAIAALADQEYLKSVQQRV
                     ARARERIYAIAQFNGFGCVPSSTNFVAVDCGRDGAYARRILEGLATHGIFVRMPGVAP
                     LNRCIRISVGTDEDLAALEEALPLAVKAAG"
     gene            3026..4198
                     /locus_tag="PKV23_02190"
     CDS             3026..4198
                     /locus_tag="PKV23_02190"
                     /inference="COORDINATES: protein motif:HMM:NF016041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HQX84272.1"
                     /translation="MNHEAAVRLFFLFSVLAFMAAWEMAAPRRRREIPRLLRWSNNLG
                     VVVIDTLLVRLSFPILAVGMAVLAAERGWGLLNVFAVPGWLAFIISLLLLDLAIYLQH
                     VMFHAVPALWRLHRMHHADLEFDVTTGLRFHPVEILLSMGLKLGIVAALGPPAIAVLV
                     FEILLNGTSMFNHSNVRLPLGLDRILRLFVVTPDMHRVHHSIHPHETNSNFGFNLPWW
                     DRLLGTYRAQPREDHEGMTIGIEQFRTPRDLWLDRMLIQPLLGPASGYPINREKETEM
                     KNSGFIDANELQQQITDGAALAILDVRGPDEFVGALGHIPGAVNIPVGDIASRLIEIK
                     ALGNKPVIIVCQTDRRSARAAEILRNENLADVRVLRGGMERWNQIGLAVKGGAPDR"
     gene            4211..>4396
                     /locus_tag="PKV23_02195"
     CDS             4211..>4396
                     /locus_tag="PKV23_02195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX84273.1"
                     /translation="MKKVPSLIPAAFLAAVVLAPGLALAQVQAAGERYGYGPGMMWDG
                     GYGLFFGPMFMILTLVAV"
BASE COUNT          872 a         1191 c         1379 g          954 t
ORIGIN      
        1 aggctttggc acggcatgat cgaaggcatc gcgccaatgg gaaaattcgg ttgaatcggc
       61 gagaaaggcg gacagcgcca gaatgggcgt tttggaggtt tttccgccaa gcgcgcgaat
      121 gccgcgggcg gcggtcacgc catccatgac cggcatttca atatcgagca gaatgacatc
      181 gtagaaggat ttttccgcct ggcggagcgc aagttcacca tcttccacgg catcaacctc
      241 aaggcccatg cgcttgagca tggcggtagt cacaatgcgc aaggcggtgg aatcctcggc
      301 gtagagaacc cgcggccttg agccatcggc atgcttggaa aatttttgtt cggggtgcag
      361 attctgactg gctagggcgg caacgggcat tcttttctgc actcacaaat cacgttcgga
      421 ggcagactag ggggttaatt attgtcgaat ggtatgcatt gccgttaact tgttgctatg
      481 actaacggga gattaatccg tcccgttctg aatcatttcg tgccgaacat gcggtcaccg
      541 gcatcgccaa gcccaggcac gatgtagccg tgggaattca ggtggctgtc aaccgccgct
      601 gtgtagatct gcacgtcagg atgttcctcg gagaatcgtt ttattccttc cggagctgaa
      661 agcaggcaga ccaggcgaat gtcctttgca cctttttcct tgaggcgggt gatggccgct
      721 gccacggaat tgccggtggc cagcatgggg tccacgacga tgacacggcg ctcttcgata
      781 tcggccggaa ccttcatgta atattcaacc ggctgcaatg tttccggatt gcggtaaatg
      841 ccgatgtggc cgacgcgggc cgagggcatg agatcaagca tgccttcgag caggccgttg
      901 ccggcgcgca gaatggagac gatcacaagt ttcttgccct tgagaatggg ggcttccatt
      961 tccgccacgg gggtttcgat ggtttgcgtc gtcatgggca ggtcgcggga tacttcatag
     1021 gcgagcagca ggctgatttc gcgcagaagc tggcggaaca cggcggacgg cgtgtgcttg
     1081 tcgcgcatca gggtgagctt gtgaaggacg agcgggtgat ccacgatcgt cagggttttt
     1141 gcaagcggcg atgtggccat ggttgcctcc tttatttgga cttttgtttc ggctttccgc
     1201 ggggcaaact caaggccaga ggccaattca tccacggctt tgacttgatt atggcccggg
     1261 atcctcctat ctgcgtaaca taacctgtta cataagagaa aagccatgac gcagcccaca
     1321 aaacccatcg ccgccggcac ttccatcggc cacgtgcacc tgaaagtggc gaatatccag
     1381 aggtcgctgg acttctattg tggggtgctg ggctttgaac tgcagcagtt ttatggcgac
     1441 caggccgctt ttgtttcggc gggcggctat catcaccacc ttggcatgaa tacctgggaa
     1501 agcctggggg gctcgccgcc gccgccgggc accacgggac tttttcacac ggcgatcctt
     1561 tatccgacgc gggccgacct cggcgatgcc ctgcggcggc tcaaggccca taacattccg
     1621 ctggatggcg cgtccgacca cggagtttcg gaagcccttt atctgcgcga tccggaccag
     1681 aatggggttg aactctactg ggaccggccc aaggacaaat ggccgcgcaa agcagatggg
     1741 agccttgcca tgatgacagc gcggcttgac ctcaccagcc tgctcgctga aggtgcctaa
     1801 ccggcggcct tcacagccag cggcagggcc tcttcgaggg cggcaagatc ctcgtcagtc
     1861 cccacgctga tcctgatgca gcggttcagg ggcgctacgc ccggcatgcg cacgaatatt
     1921 ccgtgggtgg cgagcccttc cagaattctt cgtgcatagg cgccatcgcg gccgcaatcc
     1981 acggcgacga aattggttga tgaaggaacg caaccgaagc cgttgaactg ggcaatggcg
     2041 tagattcttt cgcgggcccg ggcgacgcgt tgctgcacgc tcttgaggta ttcctgatcg
     2101 gcaagggcgg cgatggctcc ggcctggccc aggcggttca cgccgaaatg gttccggatc
     2161 ttgtcgaacg cggaaatgag atggggatgg cccagtgcat agcccacgcg gacaccggcc
     2221 atgccatagg cttttgaaaa agtgcgaaag cgcaggacat ggggattggc catatcaagg
     2281 gccggaaccg tgccttcggc aaattcatga taggcttcat ccagcatgag cacgccgcct
     2341 gcgggaatgt tgtcgatcat atcctgcacc tcgccggctt tccatgtggt ccccatggga
     2401 ttgtctggat tggcgaaata aatcagacgg gcattttcct tccttgccag gtccagcaac
     2461 gaagaaggat cctcgcggtc attcacgtaa ggagcggtga caagcctgcc gccgaaaccc
     2521 gcgacatgaa agttacaggt gggataggcg ccgagcgaac ttgccacggt gacgcctggc
     2581 tccacaaaga gcctgacggc gatgccgagc aggccgtcaa tgccttcgcc aacggtcacg
     2641 ttctccgtcc caatgccatg atgcctggca atcgcggcgc gcagatcatg aacttccggg
     2701 tcgccatatt tccacgactc gctggccgct tcctgcatgg cggcaatggc cttgggcgag
     2761 gggccgaaga cattttcatt ggcgccgatg cgggcgcgca tgggaatgcc gctgcgccgg
     2821 agaatggctt cagggccgac gaagggaacg gtggcgggaa ggctgtcgat gagaggggta
     2881 aagctggtca tggggccatg gtgctgatta atcgcgcggc atctgatata atcggtcggg
     2941 acaagtcaat gcgtgtatgc tgcattgctg agcgccgatc tatcatcaaa taaccgagga
     3001 cgtgctgcat atgccggaat atctgttgaa ccatgaagcg gcggtccggc tgtttttcct
     3061 gttttcggtc ctggccttca tggccgcctg ggaaatggcg gcgccgcggc ggcggcggga
     3121 aattccccgg ctcttgcgct ggagcaataa tcttggcgtc gtagtgatcg atacgctgct
     3181 tgtgcgcctg agctttccca ttcttgcggt gggcatggcg gtgctggccg cagagcgggg
     3241 ctgggggctg ctgaatgtgt ttgccgtacc aggctggctc gctttcatca tatcgctgtt
     3301 gctgcttgat ctggcgattt acctgcaaca cgtgatgttc catgcggtgc cggcgttgtg
     3361 gcggctgcac cgcatgcacc atgcggacct ggaattcgat gtcaccacgg gcctgcgctt
     3421 tcatcccgtt gaaatcctgc tttcgatggg gctcaagctg ggcattgtcg cagcgcttgg
     3481 cccgccggcc attgcggttc tggtttttga aatcctgctc aacgggacct cgatgttcaa
     3541 ccacagtaat gtgcggcttc ccctagggct ggaccgaatc ttgcgcctct tcgtggtgac
     3601 gccggacatg caccgggtgc atcattccat tcatcctcac gagaccaaca gcaatttcgg
     3661 cttcaacctg ccgtggtggg accgattgct gggaacctac cgggcccagc cgcgggaaga
     3721 ccatgagggc atgacgatcg gcatcgaaca attcaggacg cccagggact tgtggcttga
     3781 ccggatgttg attcaaccgt tgctgggacc ggcaagcggg tatcccatca atagggagaa
     3841 ggaaaccgaa atgaaaaact ccgggttcat cgacgcaaac gaactgcaac agcagatcac
     3901 agatggcgcg gcgcttgcca tccttgatgt gcgtggaccg gacgaatttg ttggcgcgct
     3961 tgggcatatt ccgggagcgg tcaatattcc ggttggtgac attgccagtc gcttgattga
     4021 gatcaaggcg ctgggaaaca agccggttat catagtgtgc caaaccgaca ggcgctctgc
     4081 ccgggcggcg gagattttac ggaacgaaaa tcttgccgat gtgcgtgtgc tgcgcggcgg
     4141 catggagcgc tggaaccaga ttggccttgc ggtcaagggt ggtgctcctg acagatgaag
     4201 gagtacgcag atgaaaaagg ttccaagcct gattcctgct gcatttcttg cggcggtggt
     4261 tctagcgccg ggcctggcct tggcgcaggt gcaggccgca ggcgaaagat acggctatgg
     4321 ccccggcatg atgtgggatg gcggatatgg cttgtttttt ggaccgatgt tcatgatcct
     4381 gacactggtt gccgtg
//
LOCUS       DAORVN010000072         4377 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_14939_length_4377_cov_2.994216, whole genome shotgun sequence.
ACCESSION   DAORVN010000072 DAORVN010000000
VERSION     DAORVN010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 4377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4377
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14939_length_4377_cov_2.994216"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            81..1004
                     /gene="tsf"
                     /locus_tag="PKV23_02200"
     CDS             81..1004
                     /gene="tsf"
                     /locus_tag="PKV23_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007561066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HQX84274.1"
                     /translation="MAEITAGMVKSLRESTGVGMMDCKAALAATNGDMEAATDWLRAK
                     GLAKAAKKSTRVAAEGLVGIAVNGTTGAAVEVNSETDFVARNEKFQAMVADIAKLALA
                     ADGNTEKLKTAKFPGSQHAVAEYVAEMVATIGENMGVRRTASLKVKDGVIGSYVHNQA
                     APGMGKIGVLVALESTGNKEELAAFGRLLAMHVAATNPVAMDLAGVPPETLAREKAIL
                     EEKNAGKPANVLEKIIASGLKTYAKENCLLEQAYVHDGSKSVTQVIGEVGNKAGAPVK
                     LLGYVRMQLGEGIEKAVDDFAAEVAKAAGQA"
     gene            1177..1896
                     /gene="pyrH"
                     /locus_tag="PKV23_02205"
     CDS             1177..1896
                     /gene="pyrH"
                     /locus_tag="PKV23_02205"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008192166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HQX84275.1"
                     /translation="MSKITYKRILLKVSGEVLMGNKAFGIDLDTVARIADEVIACVKA
                     GAEVALVIGGGNIFRGLAGAAKGMDRVSADHMGIMATIMNALAMQGVLKSKGIDARVL
                     SAIAMPTVCETYSPAKGIHHLEKGRVVICAAGTGLPFFTTDTGSALRAAELQCHALLK
                     GTSVDGVYAEDPKKNPKAQRFERITFNEILSRDLHIMDPAAIALARDNGIPVIVFSIR
                     EAGAVSKVLNGTGRFTLVTKD"
     gene            1900..2466
                     /gene="frr"
                     /locus_tag="PKV23_02210"
     CDS             1900..2466
                     /gene="frr"
                     /locus_tag="PKV23_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015928224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HQX84276.1"
                     /translation="MAQPVPFNAADLKRRMHGATESLKHDFGGLRTGRASATLLEPVQ
                     VDAYGAMMPLNQVASVTVPEARLLQINVWDKGLVVAVEKAIHASNLGLNPQTEGQVIR
                     LRMPDLTQDRRKELVKVAHQYAEKARIAVRNVRRDGMDQLKALEKDHAMSEDDHKKKA
                     EETQKMTDDTIKEIDASLKAKEAEIMQV"
     gene            2483..3211
                     /locus_tag="PKV23_02215"
     CDS             2483..3211
                     /locus_tag="PKV23_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007758353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HQX84277.1"
                     /translation="MLNRPPAVPRHVAIIMDGNGRWAARRGLPRSSGHKAGVDALRRA
                     VKAAADFGIEYLTIYSFSSENWSRPAAEVSFLLDLLRRFIRQDVAELHRSGVKITVVG
                     SRADLEPGIVSLLDDAERLTRDNAKLNLIVAFNYGSRQEIARAVSAIAKKIESGELSA
                     ADVSSDVISCNLDTAGIPDPDLLIRTGGEQRISNFLLWQCAYTEFVFVEDFWPDFTRE
                     IFAMALDEYRLRDRRFGGIEAQTA"
     gene            3208..4038
                     /locus_tag="PKV23_02220"
     CDS             3208..4038
                     /locus_tag="PKV23_02220"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HQX84278.1"
                     /translation="MTSAKWGDLGTRTASALILIPAVVVCAWFGGIWFKALILVLAAL
                     MAHEWVSMVHPKNYLQYALHMGAALGGAILPDLAGFEIALLVILGLALVSAVIVRFGS
                     VSPSKWSYFGVLYVGCPALALVLLRADPDYGFFAILWIFLIVWSADSLAYFAGRVIGG
                     PKLAPLISPKKTWAGLGGAVAGSALISIIFSYVSQMDGMWALAFLAGGLALVEQAGDL
                     FESSLKRFHGVKDAGGLIPGHGGVIDRVDGLIVVAVAAALIGIFRSDGALAARGLLLW
                     "
     gene            4197..>4377
                     /locus_tag="PKV23_02225"
     CDS             4197..>4377
                     /locus_tag="PKV23_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007561088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HQX84279.1"
                     /translation="MDFLNFVHMPFGYLLPFIAALTIIVFIHELGHFLVARWCGVAVE
                     VFSIGFGREIFGWNDR"
BASE COUNT          890 a         1246 c         1284 g          957 t
ORIGIN      
        1 cgtgccagcg gaataatacc caatctgaag ggccagcgat taccgccggc ccttactcca
       61 ttttttgaaa gaggatgatc atggctgaga ttacagctgg catggtgaaa agcttacgtg
      121 aatcaaccgg cgtaggcatg atggactgca aggcagccct tgccgccacc aacggcgata
      181 tggaagccgc caccgattgg ttgcgcgcca agggtctggc caaggcggcc aagaaatcca
      241 cccgcgtagc cgccgaaggc ctcgtcggta tcgcggtcaa cggcaccacc ggcgctgccg
      301 ttgaagtaaa ctcggaaacg gacttcgtcg cccgcaacga aaagttccag gccatggtcg
      361 ctgacatcgc caagttggcg cttgccgccg atggcaatac ggaaaagctg aagaccgcta
      421 aattccccgg ctcgcagcat gcggtcgccg aatatgtggc cgaaatggtg gccaccattg
      481 gtgaaaacat gggcgtgcgc cggaccgcat cgctcaaggt caaggatggc gtgattggtt
      541 cttacgtcca caaccaggcc gcccccggca tgggcaagat cggcgttttg gtcgcccttg
      601 aatcaaccgg caacaaggaa gagctggctg cctttggccg tctgctcgcc atgcatgtcg
      661 ccgcaaccaa tccggttgcc atggatctgg ccggcgttcc gccggaaacc ctggcccgtg
      721 aaaaggccat tctcgaggaa aagaacgcgg gcaagcccgc caacgtcctg gaaaagatca
      781 ttgcctctgg cctcaagacc tacgccaagg aaaactgcct gctggaacag gcctatgtcc
      841 atgacggcag caagtctgtg acccaggtta tcggcgaagt cggcaacaag gccggggctc
      901 ccgttaagct cctcggctac gtgcgcatgc agctcggcga aggcattgaa aaggcggttg
      961 atgatttcgc tgccgaagtc gccaaggccg ccggtcaggc gtgatcggaa ctcttcaact
     1021 taaaaagggc ccgcaggggc ccttttttgt tgcagtttgt gcccggcgcc atgccgaacc
     1081 ctttgattaa agtgaaaaat gtcctaagac gaatcgaact ccaatgccta gaattggcca
     1141 gatacgggac ctttcctgag aggactgtca aaacccatga gcaaaatcac ctataagcga
     1201 attcttctca aggtctcggg tgaagtgctg atgggcaata aggccttcgg cattgacctc
     1261 gatacggtag cgcgcattgc cgatgaagtt attgcctgcg tcaaggccgg cgccgaggtg
     1321 gccctggtta ttggcggcgg caacattttc cgcggcctcg ccggcgccgc gaagggcatg
     1381 gaccgggtga gcgccgacca catgggcatc atggccacca tcatgaatgc cctggccatg
     1441 cagggcgtat tgaagtccaa gggcattgat gcccgcgttc tctcagccat cgccatgccc
     1501 accgtctgtg aaacctattc gccggccaag ggcatccatc atctggaaaa gggcagggtg
     1561 gtgatttgcg ccgcaggcac cggcctgccg ttctttacca ccgacacggg ctcggccctg
     1621 cgcgctgcgg agctccaatg tcatgccctt ttaaagggga ccagtgttga cggtgtctat
     1681 gccgaggatc ccaagaaaaa cccgaaggcc cagcgcttcg aaaggatcac cttcaatgag
     1741 attctgagcc gggatctcca catcatggat cctgccgcaa tcgcccttgc acgcgacaac
     1801 gggattccgg taattgtatt ttccatccgg gaagcgggcg ccgtgtcgaa agtcttgaac
     1861 ggcactggcc gctttacctt agtgacgaag gactagctca tggcccaacc tgttcccttt
     1921 aacgccgctg acctcaagcg ccgcatgcat ggcgcaaccg aatctctcaa gcacgatttt
     1981 ggcggcctgc gcactggccg cgcctccgca actttgcttg agcctgtcca ggtggacgct
     2041 tacggcgcca tgatgccgct caaccaggtt gcctccgtca ccgttccgga agcccgcctc
     2101 ctgcaaatca atgtctggga caagggtttg gtggtggccg tggaaaaggc cattcatgcg
     2161 tccaatcttg ggttaaaccc gcagaccgaa ggccaggtca ttcgcctgcg catgccggac
     2221 ctgacacagg accgacgcaa ggagcttgtt aaggtcgccc accagtacgc cgaaaaggcc
     2281 cgcattgccg tgcgcaatgt gcgccgcgac ggtatggacc agttgaaggc gctggaaaag
     2341 gaccacgcca tgagtgagga cgaccacaag aagaaagccg aagaaaccca gaagatgact
     2401 gatgatacga tcaaggaaat cgatgcttcc ctgaaagcaa aggaagcaga aatcatgcag
     2461 gtttgaggga tagaaacacg ccatgctgaa caggccgccc gccgtcccgc gtcatgttgc
     2521 catcatcatg gatggcaacg gccgctgggc cgcgcgccgg ggcttgccgc gttcctcagg
     2581 gcacaaggcg ggcgttgacg ccctgcgccg tgcggtgaag gcggctgccg attttggcat
     2641 agagtatctc acgatctatt cctttagttc tgaaaactgg tcgcggcccg cagccgaagt
     2701 ttcatttctg cttgatctcc tccgccgttt cattcgccag gacgtggccg agttgcatcg
     2761 ctctggcgtc aagatcactg ttgtcggcag ccgcgccgac ctcgaacccg gcatcgtctc
     2821 attgctcgac gacgcggaac gcttgacccg cgataacgcc aagctgaatc tcattgtcgc
     2881 cttcaactat ggcagcaggc aggagattgc ccgtgccgtc tcggccattg ccaaaaaaat
     2941 cgagagcgga gaactctcgg ccgcggacgt ttcatccgac gtgatctcgt gcaatttgga
     3001 caccgccgga attcccgatc ccgatcttct catccgcacg ggtggcgagc agcgcatttc
     3061 caattttctg ttatggcagt gcgcttacac ggagtttgtt tttgtcgagg acttctggcc
     3121 cgactttacc cgcgagatat ttgccatggc cctagatgaa taccggctgc gggaccgtcg
     3181 tttcggcggc attgaagcgc aaaccgcatg acttcggcaa aatggggcga tctcgggacg
     3241 aggacagcct cggcgctgat cctcattccg gcagtggtcg tctgcgcctg gtttggcgga
     3301 atttggttca aggccttgat cctggtgctc gccgctttga tggcccacga atgggtctcg
     3361 atggttcatc ccaaaaacta ccttcaatac gctctgcaca tgggcgcagc cctcggtgga
     3421 gcgatcttgc ccgatttggc ggggtttgaa attgccctgc tggtcatttt gggattggcg
     3481 cttgtgtcgg ccgtgatagt ccgttttggc agtgtttccc ccagcaaatg gagttatttt
     3541 ggcgttcttt atgtcggctg tccggcgctc gccttggttc tgttgcgcgc tgacccggat
     3601 tatggcttct ttgccatact ctggatcttc ctgatcgtct ggtcggctga cagcctggct
     3661 tatttcgccg ggcgcgtcat cgggggccct aaactcgccc ccctgatttc cccgaaaaag
     3721 acgtgggccg gactgggcgg cgctgtggca ggaagtgcac tgatctcaat tattttctct
     3781 tacgtatccc agatggatgg catgtgggct ctggcatttc tcgccggcgg cctggccctg
     3841 gtggagcagg caggcgatct gttcgagtca tctctcaagc gattccatgg agttaaggac
     3901 gcaggtgggc tcatcccggg ccatggcggg gtcattgacc gggtggatgg gctgatagtc
     3961 gtggccgtgg cggccgcctt gatcggaatt ttccgcagcg acggggctct tgccgcgcgt
     4021 ggattgcttc tctggtaacc atattttgcc gaaatgttga cttaaatacg ggcttgtgtg
     4081 gttaatgaac catgaatcac gccgatggcg ctggcacgag ccttgcgaga ggtgtgtcgg
     4141 acacagcaag ttcaggcacg aacgtctgaa tatggttcgg ggagttagga cggacgatgg
     4201 attttctgaa ctttgtgcac atgccctttg gttatcttct gccatttatc gcggcgctta
     4261 caatcatagt ttttattcat gagcttggcc actttctggt ggcgcgctgg tgcggcgttg
     4321 cggtggaagt attttccatc ggttttggcc gtgaaatctt cggctggaat gaccgcc
//
LOCUS       DAORVN010000073         4374 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_14960_length_4374_cov_3.598750, whole genome shotgun sequence.
ACCESSION   DAORVN010000073 DAORVN010000000
VERSION     DAORVN010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 4374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4374
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14960_length_4374_cov_3.598750"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /locus_tag="PKV23_02230"
     CDS             complement(<1..500)
                     /locus_tag="PKV23_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019171607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HQX84280.1"
                     /translation="MSSRPELSTGEALVGLLEAYGVDTIFGIPGVHNVEMYRALPRSK
                     IKHVLVRHEQGAGFMADGYARATGKPGVCFTITGPGLLNILTPMGQAWSDSSNILVIA
                     SALDMADSAQGRGRLHEMIDQRAAAASVTNLHMRAYTPKDVRDGLARAFANFSSQRPR
                     PAYLEIP"
     gene            complement(502..1488)
                     /locus_tag="PKV23_02235"
     CDS             complement(502..1488)
                     /locus_tag="PKV23_02235"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015927238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein"
                     /protein_id="HQX84281.1"
                     /translation="MSQVLNAQNLTRYYSVSRGAFKEAAILKAVDGASFTLERGKTLA
                     VVGESGCGKSTLARLVTMIELPTSGTLSIGGHNIVGATRETLRALRPKVQIVFQNPYG
                     SLNPRQKIGAILEEPLLVNTKAPKAERQEKARAMMASVGLRPEHYDRYPHMFSGGQRQ
                     RIAIARALMLDPEVLVLDEPVSALDISIQAQVLNLLADLQEKHNLAYLFISHDLSVVR
                     HIADEVMVMYLGRVVEQGARDAVFSNPQHPYTKALLSATPTPDPMRKRERIVLKGELP
                     SPLAPPPGCTFHPRCPIAFEPCNKLNPALLAHDDRLVACHAVNPPTSNAKAA"
     gene            complement(1485..2441)
                     /locus_tag="PKV23_02240"
     CDS             complement(1485..2441)
                     /locus_tag="PKV23_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018181434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQX84282.1"
                     /translation="MPLLDIKNLTVEFKTASGYFRAVDHVSMNVDTGEVLAIVGESGS
                     GKSVSMLAVMGLLPWTARVTADRIDFDGRDLLNITPAERRQITGKDMAMIFQEPMSSL
                     NPCFTVGFQLMEALQTHLDMDKAARRKRAIGLLEEVGIPGPETRLSAFPHQLSGGMSQ
                     RVMIAMALACKPKLLIADEPTTALDVTIQAQILELLVRLQKETGMALVLITHSMGVVA
                     ETAERVSVQYAGQKVEEQPVKNLFATPRHPYTAALLAALPERAHARRLPSIPGVVPGQ
                     FDRPVGCLFSPRCIYATDLCRTTQPAMNFGALCHYPLGKAAA"
     gene            complement(2491..3162)
                     /locus_tag="PKV23_02245"
     CDS             complement(2491..3162)
                     /locus_tag="PKV23_02245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX84283.1"
                     /translation="MPDAIINALGTIAAAFVGVVTWYLASRHDRQREAAERVAHRQAE
                     ERLRDQRVLDIVVALHSEILAGIVANRRQLSKEDAEYAMAQETPFATADDTDFVFDSV
                     KDDLSILPAEVIHSVVQYYRTAKQSNLLTADLRDPYFISQTPSEKRRIIGYLLQIVET
                     QKILGEAALADLSAFAGKQGLDLTENETRAGKLLLQAREEMKLIFRKSERSVRTSKPP
                     GRRKR"
     gene            complement(3167..4060)
                     /locus_tag="PKV23_02250"
     CDS             complement(3167..4060)
                     /locus_tag="PKV23_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015338876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HQX84284.1"
                     /translation="MATETLAVAAPPGPLVAFWRSFRENRGAVIGLAVVISVVFIAIF
                     ADFIAPYSPIEQYRGFTRLPPAWIDGGNWAHPLGTDPVGRDMLSRILYGSRISLFIGL
                     SVMFVSAVLGVLLGLLAAFWGGIVDVIIIRVMDLILAIPSLVLAILIIAVIGPNLTNT
                     IVAVTIVGLPFYVRLVRASAISELTKDYVTAAKVAGVGPLRLMLSTVLPNCLAPLIVQ
                     AALGISDAILAAAGLGFLGLGAQPPTPEWGSMLADSRDLITSNPWVMAMPGIAILITV
                     LAINLMGDGLRDALDPRLKKS"
     gene            complement(4050..>4374)
                     /locus_tag="PKV23_02255"
     CDS             complement(4050..>4374)
                     /locus_tag="PKV23_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018389943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HQX84285.1"
                     /translation="EEDYVRTARAKGLSAFRVIGLHALRNALIPVVTVIGLQVGTLLA
                     GAVLTETIFSWPGIGKWLVESIGRRDYPALQGGIMLIASVVILVNLIIDLLYGLINPR
                     IRHGH"
BASE COUNT          865 a         1199 c         1491 g          819 t
ORIGIN      
        1 gggatttcga gataggcggg tcttggccgc tgactggaaa aattggcgaa ggcacgggcc
       61 aatccgtcgc gcacgtcctt gggcgtatag gcccgcatgt ggagattggt gactgatgcc
      121 gccgccgccc gctgatcaat catctcgtgc agccgtccgc ggccttgtgc gctgtctgcc
      181 atgtcgagag cggaagcgat gaccaatata ttgctggaat cggaccaggc ctggcccatg
      241 ggcgtcagaa tgttcaacag gccggggccg gtgatggtga agcatacgcc gggcttgccg
      301 gtggccctgg catagccgtc ggccatgaag cctgcgccct gttcgtggcg caccaggaca
      361 tgcttgattt tggagcgcgg cagggcgcgg tacatttcca cgttgtgcac gccgggaatg
      421 ccgaagatcg tatcaacgcc ataggcttcg agcaggccca cgagggcctc gccggttgaa
      481 agttctggac gcgatgacat gtcatgccgc ctttgcattc gaggtgggag gattcaccgc
      541 gtggcatgcc acaagacggt cgtcgtgggc aaggagggcg ggattgagct tgttgcatgg
      601 ctcgaaggca atggggcagc ggggatggaa ggtgcagccc ggcggcggcg ccagggggga
      661 aggcagttcg cccttcagca cgatgcgctc gcgcttgcgc atgggatcag gggttggcgt
      721 ggccgacagc aatgccttcg tatagggatg ctgcgggttg ctgaaaaccg cgtcgcgcgc
      781 gccttgctcc acgacgcggc cgagatacat caccatgacc tcatcggcaa tgtgacgcac
      841 tacggaaaga tcgtggctga tgaagagata ggcgaggttg tgtttttcct gaagatcggc
      901 aaggagattg agaacctgcg cctgaatgga aatatcaagg gcggataccg gttcgtcgag
      961 caccaggact tcggggtcga gcatcagggc gcgggcaatg gcgatgcgct ggcgctggcc
     1021 accggaaaac atatgggggt agcggtcata gtgttcgggc ctcagtccca cgctggccat
     1081 catggcgcgg gctttttcct ggcgttcggc ttttggcgct ttcgtgttca ccagcagggg
     1141 ctcctccaga atggcgccga ttttctgccg cgggttcaat gagccatagg ggttctgaaa
     1201 aacgatctgc accttgggcc gcaaggcgcg cagggtttcc cgggtggcgc cgacaatgtt
     1261 gtggccgccg atgctgagcg tgccggaggt cggcagttca atcatggtga cgaggcgggc
     1321 aagggttgat ttaccgcagc cggattcgcc cacgacggcc aatgtcttgc cgcgctcgag
     1381 cgtgaagctg gcgccatcga cggctttcag tatggcggct tccttgaagg ctccgcgcga
     1441 aacgctgtag tagcgggtga ggttttgggc attcaggacc tggctcatgc ggcagccttt
     1501 ccgagcggat aatggcacag ggcgccaaag ttcatggcgg gctgggtcgt ccggcagagg
     1561 tcagtcgcat agatgcagcg cggcgaaaac aggcagccca ccgggcggtc aaactgtccc
     1621 ggcacgacac cgggaatgga gggcaggcgg cgggcgtggg cgcgctcggg cagggccgca
     1681 agcagggccg ccgtataggg atggcgtggg gttgcgaaaa gatttttgac cggctgctcc
     1741 tcaaccttct ggccggcgta ttgcacgctg acccgctcgg ccgtttcggc cacgaccccc
     1801 atggaatggg tgatgagcac cagggccatg ccggtctcct tttgcaggcg caccagaagt
     1861 tcgaggatct gcgcctggat ggtcacatcg agtgcggtgg tgggttcatc ggcgatcagc
     1921 agtttgggtt tgcaggccag cgccatggca atcatcacac gctggctcat gccgccggaa
     1981 agctggtgcg gaaaagcgga aaggcgcgtt tcaggacccg gaatgccgac ttcctccaga
     2041 agcccgatgg cccgcttgcg ccgggcagcc ttgtccatgt cgaggtgggt ctgcaaggct
     2101 tccatgagct ggaaccccac ggtgaagcag gggttcaggc tcgacatggg ttcctggaaa
     2161 atcatcgcca tgtccttgcc ggtgatctgg cggcgctcgg cgggggtgat gttcaggaga
     2221 tcgcggccgt caaagtcgat cctgtcggcg gtaaccctgg cggtccacgg caacagcccc
     2281 atgacggcga gcatggacac ggacttgccg gagcctgatt caccgacgat ggccaaaacc
     2341 tcgcccgtat ccacattcat cgagacatgg tcgacagcgc ggaaatagcc gctggcggtc
     2401 ttgaattcta cggtcaggtt cttgatgtcg agcagtggca tgcgggttgt tttttccttc
     2461 gtttatccta tattcatggg atcgtgataa ctagcgtttt cgtctcccag gaggctttga
     2521 tgtccggacc gatcgttccg atttcctgaa gataagcttc atctcttccc tggcttgcaa
     2581 cagcagcttg ccggcgcggg tttcattctc cgtcagatca agcccctgct tgccggcgaa
     2641 ggccgacagg tcagcgaggg ctgcttcgcc caggattttc tgggtttcca caatctgcag
     2701 gagatagcca ataatgcggc gcttctcgga aggggtctgg ctgatgaaat aggggtcgcg
     2761 caggtcggcg gtcagcaggt tcgattgttt ggcggtgcgg taatactgca ccacggagtg
     2821 gatcacttcc gccggcaata tcgaaagatc atccttcacc gagtcaaaga cgaaatccgt
     2881 gtcatcggcc gtggcaaagg gagtttcctg ggccatggca tattccgcat cttccttgct
     2941 cagctggcgc cggtttgcca cgatgcccgc gaggatttcc gaatgcagcg ccactacaat
     3001 atccaggacg cgctggtcac gcaggcgttc ctcggcctgg cgatgggcca cgcgttccgc
     3061 cgcctcgcgc tgccggtcgt ggcgcgaggc gagataccag gtgactacgc ccacgaatgc
     3121 ggcggcgatg gtaccgagcg cgttgatgat ggcgtcgggc atggcctcaa ctctttttca
     3181 ggcggggatc gagtgcgtcg cgcaggccgt cgcccatgag gttgattgca agaaccgtga
     3241 tcaggatggc aatgccgggc atggccatga cccaggggtt ggaggtgatg agatcgcggg
     3301 aatcggccag catggaaccc cactccgggg tgggcggctg ggccccgagc ccaaggaaac
     3361 ccaggcccgc agcggccagg atggcatcgg atatgccaag ggctgcctgg acgatgagcg
     3421 gggcgaggca gttgggcagc acggttgaga gcatcagccg gagcggaccc acgcctgcga
     3481 ctttggcggc ggtgacatag tccttggtca attcgctgat tgccgaggcg cgcaccaggc
     3541 gcacatagaa aggcaggccc acaatggtca cggccacgat ggtgttggtc aggttggggc
     3601 caatgaccgc gatgatgagg atggccagca ccaggctcgg aatggcaagg atcaaatcca
     3661 tgacgcgaat gatgatgaca tcgacgatgc cgccccagaa agcagcaagc aggccaagca
     3721 gaacgcccag cacggcagag acaaacatga ccgaaaggcc aatgaagaga gagatgcgcg
     3781 aaccgtaaag gatgcgtgaa agcatgtcgc gtcccacggg atcggttccg aggggatggg
     3841 cccagtttcc gccgtcgatc caggcgggcg gcagtctggt gaagccgcgg tattgttcga
     3901 tcggactgta gggggcgatg aaatcggcaa agatggcaat gaagacaacg gagatgacca
     3961 cggccaagcc aatgacagcg ccgcgatttt cgcggaacga gcgccagaag gcgaccagcg
     4021 gcccgggcgg cgcggcgacg gcaagcgttt cagtggccat ggcggatccg cggattgatc
     4081 aggccataaa gaagatcgat gatcaggttg acgagaatga ccacgctggc aatgagcatg
     4141 atgccgccct ggagggccgg ataatcgcgc cggccgatgg attcaaccag ccatttgccg
     4201 atgcccggcc aggagaaaat ggtttcggtg agtacggcgc cagccagaag cgttcccacc
     4261 tgcaagccga tgacggtgac gacgggtatg agcgcgttgc gcagggcatg aagaccaatg
     4321 acgcggaagg cggaaaggcc tttggccctt gcggtgcgca catagtcctc ctcc
//
LOCUS       DAORVN010000074         4374 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_14961_length_4374_cov_3.053253, whole genome shotgun sequence.
ACCESSION   DAORVN010000074 DAORVN010000000
VERSION     DAORVN010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 4374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4374
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14961_length_4374_cov_3.053253"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..903
                     /locus_tag="PKV23_02260"
     CDS             <1..903
                     /locus_tag="PKV23_02260"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006202700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HQX84286.1"
                     /translation="AKETGADAIAHGATGKGNDQVRFELTAYALNPDIKIIAPWREWD
                     FKSRTDLINFAEAHQIPVSKDKRGEAPFSVDANLLHSSSEGKVLEDPWVEAPPYVYQR
                     TIAPEDAPDKATYIEIEFKKGDPIAIDGKAMSPATLFTKLNELGHDNGIGRLDLVENR
                     FVGMKSRGVYETPGGTILSVAHRAMESLTLDREAAHLKDSFMPRYAELVYYGFWYSPE
                     REMLQAMVDKSQEHVEGIVRLKLYKGNVIVVGRKSPKSLYKDTLVTFEDDRGAYDQKD
                     AEGFIRLNALRLRTLAARKRQPSP"
     gene            900..2129
                     /locus_tag="PKV23_02265"
     CDS             900..2129
                     /locus_tag="PKV23_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018116626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HQX84287.1"
                     /translation="MTTLAVTVFLLVYLGMALGRVPGLAIDRTGVALLGLIVLLASGD
                     LSLDEAGRAVDMPTIALLFALMILSAQFEMSGFYGLVASRVTHAARNPRLLLLAVVLA
                     TGALSAVLSNDVVVFALTPLICAGLMAQGLDARPYLVGLAGAANAGSAATLIGNPQNI
                     LIGQAGGLDFWGYILLALPPVLLSLGFVYGAVLLTWRKGLRATPIADVDLSSPLVDRY
                     QLAKGLVAVIVLIGLFLTPLPRELSALAVAGFLLLSRKLSSREMIGAADWHLLLLFVC
                     LFGVTAAFAKTGLAQEGLKELADFGFLPERLSVMGPLMLAASNTIGNVPTIILILKLL
                     PALPPGTLTALALLSTLAGNLLLTGSLCNIIVAERAASAGARLSFLDFARSGVIMTLA
                     SMGVTIWWLWFLGVMPL"
     gene            complement(2126..3235)
                     /gene="leuB"
                     /locus_tag="PKV23_02270"
     CDS             complement(2126..3235)
                     /gene="leuB"
                     /locus_tag="PKV23_02270"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009490822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HQX84288.1"
                     /translation="MASHNLFLLPGDGIGPEVMAEVEAIIAWMDGKGIAKFKTDRGLV
                     GGCAYEAHGKAISEEDMEKAQGADAVLFGAVGGPRWDKVAYVHRPEAGLLRLRKDLGL
                     FANLRPAICYPALAEASSLKRELVDGLDILIVRELTGGVYFGEPKTITDLGNGQKRAI
                     DTQIYDTYEIERIARVAFELARTRKNRVASAEKRNVMKTGVLWNEVVTRIQAESYSDV
                     ALEHVLADNCAMQLVRWPKQYDVIVTDNLFGDILSDVAAMLTGSLGMLPSASLGAEDP
                     KTKRRKALYEPVHGSAPDIAGKALANPIAMIASFGMCLRYSFGLIDWADNVDTAIANV
                     LAKGLRTKDIAAKDEKAVTTREMGAAILKELKALA"
     gene            complement(3303..4145)
                     /locus_tag="PKV23_02275"
     CDS             complement(3303..4145)
                     /locus_tag="PKV23_02275"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HQX84289.1"
                     /translation="MAYRTILVSLNELNRLPQLLSAVATIARNFNAHVSGLYVIPAVQ
                     LYPSMGFEAVPQVFDGNQTYFKDNQEKVRAAFEEAMRKEGLNSDFHVVQGATSSIAEE
                     VVNKGRSADLLIISATNPDETTGVESDFVEQTLMAAGRPIMVLPFKGETKLNLDDVVL
                     GWNGDREAARAAFDALPFLKAAKKVRIVRTDPQKDPSLKGSIAGADLAETLARHGVKV
                     EAASFPTAGDDAGHALLRCADDNGCDLIVMGAYGHSRLREYIFGGATQYVLNHLNRPV
                     LMSH"
     gene            complement(4218..>4374)
                     /locus_tag="PKV23_02280"
     CDS             complement(4218..>4374)
                     /locus_tag="PKV23_02280"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006172651.1"
                     /note="NADH-dependent; catalyzes the conversion of
                     pyrimidines to 5,6-dihydro compounds in pyrimidine
                     degradation; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HQX84290.1"
                     /translation="NLCVNVCPVEDCITMVQQTSGTDPRTGRKINRDYANWTTHPNNP
                     MALKAAE"
BASE COUNT          803 a         1235 c         1402 g          934 t
ORIGIN      
        1 gccaaggaaa cgggggctga tgccattgcc catggggcca ccggcaaggg caatgaccag
       61 gtgcgctttg aactcacggc ctatgcgctc aaccccgaca tcaagatcat cgctccctgg
      121 cgcgagtggg acttcaagag ccgcacggac ctgatcaatt ttgccgaagc ccaccagatt
      181 ccggtgtcca aggacaagcg cggcgaggcg ccgttctcgg tggatgccaa tctgctgcat
      241 tcctcatcag aaggaaaagt cctggaagat ccctgggtgg aagcgccgcc ctatgtgtat
      301 cagcgcacca ttgcgccgga ggatgcgccg gacaaggcca cctatatcga aatcgaattc
      361 aagaagggcg accccatagc cattgatggc aaggccatgt cgcccgccac gctgttcacc
      421 aagctcaatg agctaggcca cgacaacggc attggccggc ttgatctggt ggagaaccgt
      481 ttcgtcggga tgaaatcgcg gggtgtttat gagacgccgg gcggcacgat cctgtcggtg
      541 gcgcaccggg ccatggaaag cctgacgctg gaccgggagg cggcccatct caaggacagt
      601 ttcatgcctc gttatgcgga gctggtttac tacggcttct ggtattcgcc ggagcgtgaa
      661 atgctgcagg ccatggtcga caaatcccag gagcatgtgg aagggattgt ccgcctcaag
      721 ctctacaagg gcaatgtgat tgtggttggc cgcaagtcgc cgaagtcgct ttacaaggat
      781 acgctggtga ccttcgagga tgaccgcggg gcctatgacc agaaggatgc ggaagggttc
      841 atccgcctga acgcgctgcg gctgcggacc ctagcggcaa ggaagcgcca gccttctccg
      901 tgaccacgct ggccgtcacc gtttttttgc tggtctatct cggcatggcg ctggggcgcg
      961 tgccgggact ggcaatcgac cgtacgggcg tggcgctgct cgggctgatt gtgctgctgg
     1021 cctcgggcga tctcagcctt gatgaggcag gccgggcggt cgacatgcca accatcgcat
     1081 tgctgtttgc actgatgatt ctctcggcgc aattcgagat gagtggtttc tatggcctgg
     1141 tagccagccg cgtcacccat gcggcgcgaa atccgcgcct gctgctgctg gcagtggtgc
     1201 tggcgacggg ggcgctctcc gcggtgctgt ccaatgacgt tgtggttttt gcgcttacgc
     1261 cgctgatctg cgctgggcta atggcgcagg gactcgatgc cagaccctat ctcgtcgggc
     1321 tagcgggagc cgccaatgcc ggttcggcgg caacccttat cggcaatccg cagaacatcc
     1381 tgatcgggca ggcgggcggc ctcgatttct ggggctatat cctgcttgcc ctgccgccag
     1441 tgctgctgtc tcttggcttt gtctatggtg cagtgctgct gacctggcga aagggtctga
     1501 gggcgacacc cattgccgat gttgatctgt cttcgccact tgttgaccgt tatcagcttg
     1561 ccaagggcct cgtggcggtc attgtcctga tcggcctttt tctcacgccc ctgccgcggg
     1621 aattgagcgc gctggcggtg gcaggcttcc tgctcctgtc gcgcaagctt tcatcacggg
     1681 agatgatcgg cgcggccgac tggcacctgc tcctgttgtt tgtctgccta ttcggtgtca
     1741 cggcggcctt tgccaagacg ggccttgccc aggaaggtct caaagagctg gcggacttcg
     1801 gcttcttgcc ggaacgcctg tcggtgatgg gccccctgat gctggcggcg tccaacacga
     1861 tcggcaatgt gcccacaatc atccttattc tcaagctgct gccggcgctg ccgcctggaa
     1921 cgctcacggc tctggccctg ctttccaccc tggccggaaa cctgctgctc acgggctcgc
     1981 tctgcaatat catcgtggcg gagcgcgcgg cgagcgccgg cgccaggctt tcgttcctcg
     2041 attttgcccg ctccggagtc atcatgacac tggccagcat gggcgtcacc atctggtggc
     2101 tgtggtttct tggtgtgatg ccgctttagg ccagcgcctt taattctttc aggattgccg
     2161 cgcccatttc cctggtggtc acggccttct cgtccttggc ggcgatgtcc ttggtgcgga
     2221 gccctttggc caggacattg gctatggcgg tgtcgacatt gtcggcccag tcgatgagcc
     2281 caaaggaata acgcaggcac atgccgaagg aagcgatcat ggcaatggga ttggccagcg
     2341 cctttccggc gatgtcgggg gcagaaccgt gcaccggttc atagagcgcc ttgcggcgtt
     2401 tggttttggg atcttcagcc ccgagagaag cggacgggag catgccgagg gaaccggtga
     2461 gcatggcggc cacatcggag agaatatcgc cgaacaaatt gtcggtgacg ataacgtcat
     2521 actgtttggg ccagcgcacg agctgcatgg cgcagttgtc ggcgagtaca tgctcaagag
     2581 cgacatcgga atagctttct gcctgaatgc gggtgacgac ttcgttccac agcacgccgg
     2641 tcttcatcac gttgcgcttt tcagccgagg ccacgcggtt cttgcgggtg cgggcgagtt
     2701 cgaaagcgac gcgggcaatg cgctcgatct cataggtgtc atagatctgc gtatcgatgg
     2761 cgcgtttctg gccattgccg agatcggtga tggttttggg ttcgccgaaa taaaccccgc
     2821 cggtcaattc gcgcacgatc aggatatcaa gaccgtcgac aagttcgcgc ttgagggacg
     2881 aggcttcggc aagcgccggg tagcagatgg cgggccgcag attggcaaaa agacccaaat
     2941 ccttgcggag gcgaaggagg ccggcttccg ggcggtggac ataggcgacc ttgtcccagc
     3001 gggggccgcc gacggcgccg aagagaaccg catcggctcc ttgcgccttt tccatatcct
     3061 cttcggaaat ggccttgcca tgggcctcat aggcgcagcc gccgacaagg ccacggtcgg
     3121 tcttgaactt ggcgatgcct tttccatcca tccaggcaat gatggcttca acctcggcca
     3181 tgacttcggg gccgataccg tcaccgggga ggaggaaaag attgtgggaa gccatggcat
     3241 atccgtttcg cgagagcaat ttcgcggcga tgggtagccg tcccgaaggt ttagtgcaag
     3301 gcttaatgcg acatcaggac cgggcggttc agatggttga ggacgtactg cgttgcgccg
     3361 ccaaaaatat actcgcgcag gcgggaatgg ccataggccc ccatgacgat aagatcacag
     3421 ccattgtcgt cggcacagcg cagcagggcg tggcccgcat catcgccggc ggttggaaag
     3481 cttgcggcct ccaccttgac cccgtggcgg gccagggtct cggcaaggtc agctccggcg
     3541 attgagccct tgagcgacgg gtctttctgg ggatcggtac ggacgatgcg caccttcttt
     3601 gcggccttga ggaacggcag ggcgtcaaag gcggcgcggg cggcctcgcg gtcgccattc
     3661 caacccaaca ccacgtcatc gagattcagt ttggtttcgc ccttgaaagg caggaccatg
     3721 atggggcggc ctgccgccat gagggtctgc tcaacaaagt cggattcgac accggtggtt
     3781 tcatcggggt tggtggcgga aataatcagg aggtcggcgc tccggccctt gttgacgact
     3841 tcctcggcaa tgctcgaggt ggcgccctgg acgacatgaa aatcagaatt caggccttcc
     3901 ttgcgcatgg cctcttcaaa ggcggcgcga accttttcct ggttatcctt gaaataggtt
     3961 tggttgccat caaagacctg cggcaccgct tcaaagccca tggacgggta gagctgcaca
     4021 gcaggaatga catagaggcc agagacgtgg gcgttaaagt tgcgggcaat ggttgcaacg
     4081 gcgctgagaa gctgcggcag acggttcagc tcattcagtg aaacgagaat tgttctgtag
     4141 gccatgacat gtcctccgta aggcgtgaag gcagtgtgct tgacttaaca taggttgtcc
     4201 ttgtgtgaga tcaagaacta ttcggcggcc ttgagggcca tgggattgtt cgggtgagtg
     4261 gtccagttgg catagtcccg gttgatcttg cggccggttc tggggtcggt tccactggtt
     4321 tgctgcacca tggtgatgca atcctcaacc gggcagacgt tgacgcacag gttg
//
LOCUS       DAORVN010000075         4350 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_15130_length_4350_cov_2.216298, whole genome shotgun sequence.
ACCESSION   DAORVN010000075 DAORVN010000000
VERSION     DAORVN010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 4350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4350
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15130_length_4350_cov_2.216298"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="PKV23_02285"
     CDS             <1..210
                     /locus_tag="PKV23_02285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX84291.1"
                     /translation="IVVAHTNVAMQEMVAKGLDLCAGVQAVVQMSMRMYADLIGDDEE
                     AIRQARKFLDDWERDLIDAPDGLTH"
     gene            complement(462..1121)
                     /locus_tag="PKV23_02290"
     CDS             complement(462..1121)
                     /locus_tag="PKV23_02290"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HQX84292.1"
                     /translation="MAKPPVIEDRQVEHLLKATAAYSRVPLRDTALLLTLYGTALSVT
                     ELATVTVGDYLDAKGNVRVTSAVRVDVAHNGEERPLFWSNKRVVSALDAYLAWRVERK
                     HGVTVKKGAYRGLDPESALFLTEDGGAYALTKRMLPSGVWSYSCNTLGAYISRLHANA
                     GIEGGSAQSARRTFAVKQHRQGRDLVHIAAILGHKSVSTTKRLVEGDPVRLADIVARA
                     V"
     gene            complement(1125..1856)
                     /locus_tag="PKV23_02295"
     CDS             complement(1125..1856)
                     /locus_tag="PKV23_02295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HQX84293.1"
                     /translation="MTTIADIKNHKTNGPWRGLEKLAHEMVQDASTVAKSTLLPVLGG
                     GTRLMLAMEHRISDDIDLFIRDPQWIGFLTPRLNDKFEHLINTYDEGATSLKLKFNDG
                     EIDFIVSMSLLGLDNKVSSECLFPLEPVAEVLAKKLFYRGWALTPRDLFDWHAIERHV
                     PLLDTHELMLAELIAARVDGIRMALDQMGTSKQSAIVWQEIRTHELPDLKQTIQWAQG
                     RLGKFQAMSEQAAKDKASGRRPVER"
     gene            complement(1853..2128)
                     /locus_tag="PKV23_02300"
     CDS             complement(1853..2128)
                     /locus_tag="PKV23_02300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX84294.1"
                     /translation="MAVTHLNAPYGAIVSPSDLIESLSQGELRAASPKAAAILSYLFI
                     EVEPRLVMRCAMESGSSLLKANQLYSDTIKGGAPRSALWESSIKALL"
     gene            complement(2198..2386)
                     /locus_tag="PKV23_02305"
     CDS             complement(2198..2386)
                     /locus_tag="PKV23_02305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX84295.1"
                     /translation="MNFTVRFDADTGALSEAYALDDNGQKWGCAPQGRYPGQEADLKV
                     FEVAWALVPRLVRGWLRV"
     gene            2624..2893
                     /locus_tag="PKV23_02310"
     CDS             2624..2893
                     /locus_tag="PKV23_02310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX84296.1"
                     /translation="MNCPAGPQILTYTTNAMVGSGGTNTAVVNLLWTNLMGSAPSAAD
                     AQPFVNLLNQGAFTRRAMTSAVAELLDQKVGVTGVASYDFTVFGV"
     gene            3152..3388
                     /locus_tag="PKV23_02315"
     CDS             3152..3388
                     /locus_tag="PKV23_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019224016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin MazE family protein"
                     /protein_id="HQX84297.1"
                     /translation="MAAASINARVQKHRDALRTAGLRPVQIWVPDTRRPDFAAECRRQ
                     SQIASQADMADTDMDMDMEHFMDATLADLDGWTE"
     gene            3385..3711
                     /locus_tag="PKV23_02320"
     CDS             3385..3711
                     /locus_tag="PKV23_02320"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009873354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HQX84298.1"
                     /translation="MMRGDFVTISLQGDFGKPRPALIIQSDRFNEHATVTVLPVTGTM
                     VAAPLLRVTVQPDASNGLQKPSQVMVDKAMTVKSDKVGPAFGRIGADALIVVERCLAV
                     FLGIAK"
     gene            4086..4289
                     /locus_tag="PKV23_02325"
     CDS             4086..4289
                     /locus_tag="PKV23_02325"
                     /inference="COORDINATES: protein motif:HMM:NF021474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HQX84299.1"
                     /translation="MRTTVTIDDALYQEALEVAEPGMDKADLIREAIKTFVRVQAAKR
                     LASLGSATPDMPDISRRRETAPQ"
BASE COUNT          935 a         1259 c         1277 g          879 t
ORIGIN      
        1 attgtggttg ctcatacgaa tgtagctatg caagaaatgg ttgccaaggg cttggacctc
       61 tgcgctgggg tgcaagctgt tgtgcaaatg agcatgcgga tgtacgccga tttaatcggt
      121 gatgatgagg aggcaatccg acaagccagg aagtttttgg atgattggga gcgcgactta
      181 atcgacgctc cagatggcct aacacactga actggtaaac cgaggaaagc acgctgcgtc
      241 cgtccgtaca cccccaactc ttgcagcgct tgagcgaaca gagtgggcat agaaggtcat
      301 ggtgcgtgtg cacccctcgc tcgcacaagt gcgctcgatg aggcaatctg ccaagtcggc
      361 cttgcccgtc gcaaaaatcc gcagggccct ggccagctga tcagcgcgct cgaccaccag
      421 ctgcttggtc cgcaaaatgg cttcccgcgc ctgtcccgtc atcacacagc cctcgccaca
      481 atgtctgcaa ggcgcacggg atcgccctca accagccttt tcgtggtgct cacgctcttg
      541 tgcccaagga ttgcagcgat atgcacgagg tcgcggccct ggcgatgttg cttgacggcg
      601 aaggtgcggc gagcggactg agcgctcccg ccctcgatcc cagcgtttgc gtgcaggcgc
      661 gagatgtacg cccccaaggt gttgcaggaa tagctccaga cgcctgacgg gagcattcgc
      721 ttggtcagcg catacgctcc accgtcttcg gtcaggaaca aggcgctctc ggggtcgagc
      781 ccacggtagg cgcccttctt caccgtgacg ccgtgcttgc gctccacgcg ccaggcgaga
      841 taggcatcca gtgccgacac aacgcgcttg ttcgaccaga acagcggacg ctcttcaccg
      901 ttgtgcgcaa catcgacgcg caccgcgctc gtgacgcgca cgttgccttt ggcatcaagg
      961 tagtcgccca ccgtgacggt ggcaagttcc gtgaccgaga gggcggtgcc gtagagagtc
     1021 aggagcaatg cggtgtcgcg cagtggaacg cggctgtagg ctgcggtagc tttcagcagg
     1081 tgctcgacct gtctgtcttc gatgactggg ggttttgcca tggactatcg ctccacaggc
     1141 cgacgacccg aagccttgtc tttcgccgcc tgctcactca ttgcctgaaa tttccccaat
     1201 cgcccttgag cccactgaat ggtttgcttg agatcgggca gctcatgtgt gcgaatttcc
     1261 tgccagacta tcgccgattg tttcgatgtc cccatctggt ctagcgccat gcgaatgccg
     1321 tccactctgg ccgcgatcaa ttcagccagc atcagctcat gggtatccaa aagcggcaca
     1381 tggcgttcaa ttgcatgcca gtcaaacagg tcacgcggcg tgagagccca cccacgataa
     1441 aaaagcttct ttgcgagcac ttccgccacc ggctcaagtg ggaagagaca ctcgctggaa
     1501 accttgttgt cgagtccaag cagggacatg ctgacgatga agtcaatttc gccatcgtta
     1561 aatttgagct ttagggaggt ggccccctcg tcgtaggtgt tgatgaggtg ctcaaatttg
     1621 tcattcaggc gcggggtgag aaatccgatc cattgaggat cacggatgaa caaatcgatg
     1681 tcgtcactga tccgatgctc cattgccaac ataagccttg ttccaccgcc caatacagga
     1741 aggagagtgc ttttggcaac cgtggatgcg tcttggacca tctcatgtgc aagcttctca
     1801 agccctctcc atggaccatt ggttttgtga ttcttgatgt ccgcaatagt ggtcatagga
     1861 gagccttgat tgatgattcc caaagggcgc tgcggggcgc accgcccttg atagtgtccg
     1921 aatagagttg gttggccttc agcagcgacg aacccgactc catcgcgcac cgcatcacca
     1981 accgtggctc cacttcaata aagaggtagc tcaaaatcgc agcggctttg ggtgaagcag
     2041 cacgaagctc cccctggctc agtgattcga tgagatcgct gggagaaaca atcgctccgt
     2101 atggcgcgtt taagtgcgtc acagccatcg caataccgtg ttgatgtggg cataaatttg
     2161 gcatgcattt gccttaaatc gtgcaattac attgagtcta cacgcgaagc cagccccgaa
     2221 caagccgggg caccagcgcc cacgccacct cgaaaacctt cagatcggct tcctgtcctg
     2281 gataacgacc ttgtggcgcg cagccccatt tttggccatt gtcatccagg gcgtaagcct
     2341 cagacaaagc accagtgtca gcgtcaaacc gcaccgtaaa gttcataggt tctccagaaa
     2401 gaactgcacg caacatgcgt gcttggttca gttggagttg ccatgacgct gcgaccaggc
     2461 gccccaatcg ggaggcgatg aaccgctatt agcggagcag cgcggggcag tggggaaatg
     2521 ctgcgcgcgc cctcccgaga ccggcgccca tgcgcacttg ccggaaagag cggggcgaaa
     2581 ccctggatgg cagaatggaa caaccgcgtc ctgagcgaac ttgttgaatt gcccggccgg
     2641 cccgcaaatc ctgacttaca caaccaatgc catggtgggc tccggcggca ccaacacagc
     2701 cgtggtgaac ctgctctgga ccaacctcat gggcagtgcc ccgagcgcag cggacgccca
     2761 gcccttcgtg aacctgctca accaaggcgc cttcacccgc agggccatga ccagcgcagt
     2821 ggccgaactg ctggaccaga aggttggcgt gacgggtgtg gccagctacg acttcactgt
     2881 gttcggggtt tgaggggggc gggggcagtc gccgcgcgct ccgcccaaca aatgaatgaa
     2941 caaagccctc tcacaagggc tttgttcatt gcgctaagca gcgcggccca gtagtaggca
     3001 ttgaggtcga tccaggccgg gccaagttga cggcggcgcg acggagcgac tatccatggg
     3061 tcatggctgc agcaacccca aatttcaggg gctggacaag ttacatgaaa catatcacaa
     3121 tgttccatgt aacaatagaa aggggtttga aatggcagca gccagcatca atgcacgggt
     3181 gcaaaagcac cgcgacgcgc tgcgtacggc gggcctgcgg ccggtgcaga tttgggtgcc
     3241 ggacacgcgc cgaccagact ttgctgcgga gtgccgccgc cagtctcaga tcgcatctca
     3301 agccgacatg gccgacacgg acatggacat ggacatggag catttcatgg atgccacctt
     3361 ggccgatctg gatggctgga cggaatgatg cggggcgatt tcgtaaccat ctccctgcag
     3421 ggtgactttg gaaaaccgag gcctgccctg attatccagt ccgaccgatt caacgagcat
     3481 gcgactgtga cggttctgcc ggtgacgggc acgatggtcg ccgcgccgct gctgcgcgtc
     3541 accgtccagc cagacgccag caacggtttg caaaagcctt cccaggtaat ggtggacaag
     3601 gccatgaccg tgaagagcga caaggtgggg ccggcttttg ggcgcatcgg ggctgatgcg
     3661 ctgattgtgg ttgagcgctg cttggccgta ttcttgggaa tagcaaaatg aagcggactt
     3721 actcggaggc caagtcctcc gcatctagct gcgacgcctt cagcagcagc gcccatgcac
     3781 tttgacaaac ttcgtcgccg cgcttgactt gtgtacgcat tgattcacaa ccggcgactt
     3841 tgaccatgga agccacgacg gcagggatcc gcgaatttca cgcgggcatg gcgcaaatac
     3901 atgcgcccag atgagttgct gtctgcacag ggcgttgacg tcgaggacac gatctgctag
     3961 accaataggg cgcattccag ttggatgcgg cctcaaccgc cctttgcacc aaatggatgg
     4021 gtaacccctc taagatgcgt tatttgatgc acaatgactc catatttgat gcaggaaggg
     4081 catttatgcg aaccaccgtg acaatcgatg acgccctgta ccaagaggcc ttagaggttg
     4141 cagagccagg aatggacaag gccgacctga tccgcgaggc catcaagacc tttgtgcgtg
     4201 ttcaagctgc caagcggctc gcgtctctgg gcagcgcgac gccagacatg ccggatatct
     4261 cgcggcgacg tgaaactgct ccgcagtgat gcgcgtgctg gtcgatacct cggtctgggt
     4321 tgagcacttc cgccatggga gtgcgccttt
//
LOCUS       DAORVN010000076         4298 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_15454_length_4298_cov_3.872732, whole genome shotgun sequence.
ACCESSION   DAORVN010000076 DAORVN010000000
VERSION     DAORVN010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 4298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4298
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15454_length_4298_cov_3.872732"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /locus_tag="PKV23_02330"
     CDS             complement(<1..297)
                     /locus_tag="PKV23_02330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX84300.1"
                     /translation="MVPDRTPGKGDLRVFLTLESLQPQFAAYLGTPTRARGYPPYAGQ
                     HALIIEVAPGLMIERVIDLALKADPDLEPGILFVERQFGILEIHGSDKERLLAAG"
     gene            complement(312..596)
                     /locus_tag="PKV23_02335"
     CDS             complement(312..596)
                     /locus_tag="PKV23_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HQX84301.1"
                     /translation="MSSNAIGMVETKGYVAALAAADAMVKAANVTIIGREEVGDGLVA
                     VIINGDVGAVKAATEAGAEVAGQVGTLVSVHVIPRPHADVSKHFTSGTAK"
     gene            complement(625..882)
                     /locus_tag="PKV23_02340"
     CDS             complement(625..882)
                     /locus_tag="PKV23_02340"
                     /inference="COORDINATES: protein motif:HMM:NF015289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HQX84302.1"
                     /translation="MLKATVTGRIWSTKKLGDVPTGSILEVQVEDSSTRLLAFDPLGC
                     GDGEKVIVATGSVAAAWFPGKAPPIDALIIGSIDESSEKKK"
     gene            complement(882..1262)
                     /locus_tag="PKV23_02345"
     CDS             complement(882..1262)
                     /locus_tag="PKV23_02345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX84303.1"
                     /translation="MKAETSQELRGLVREVLKEAMANRAAPKQGAETIRIGSDADLQA
                     FVARISSPGVIESVRAGKLRFTLGHTAPAPTGSGDILEGVISEQKISALAGKGAVILG
                     PTAVLTPLARDRARKLGLKIERRR"
     gene            complement(1259..1858)
                     /locus_tag="PKV23_02350"
     CDS             complement(1259..1858)
                     /locus_tag="PKV23_02350"
                     /inference="COORDINATES: protein motif:HMM:NF013129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HQX84304.1"
                     /translation="MAILRSFIFLDQLQPQTMCYLGSWIKGSLPRRNMAAQIIEVAPG
                     LDIEALTDIALKEADVQGGILVVERQFGYLEIHSRDIAAVKASAEAVLAALGKNSSDA
                     MPPKILASKIITRIDPNHAFLINRNKGGSMILAGETLFVLETEPAAYAILASNAAEKA
                     ANIKIVDYRMIGATGRLYLSGTESEVKAAAGAAEQALEL"
     gene            complement(1862..3373)
                     /locus_tag="PKV23_02355"
     CDS             complement(1862..3373)
                     /locus_tag="PKV23_02355"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HQX84305.1"
                     /translation="MSEALLKVPDAALEQARMMLDRARWGATRFQKLDRSTTQRIVNA
                     VAAAAHGKAQHYAEWAVRETGMGVVAHKRMKNEACSKGLAETYSGENFVSPRIDEAKK
                     IVELPRPAGVIFALIPVTNPVATVYFKTLLALMTRNAIILSPHPGAKACCADAARMLA
                     EAAKAAGAPDGVIQVLEEPNIPLIEKLIADERIDLVVATGGPSVVKAAYRSGNPAYGV
                     GPGNAPVLVDDTADMRLAAKRIVASKAFDNSILCTNESAVLAFSSVAESLLASMKAEG
                     AHICTAEEAQKLRQLLFTEIGFNVGMIGRDASVIAKAAGFPAPNARILVTPVDLVQPE
                     EKLVREKLCPVLAFARVGNIDQAISAARSMMRNSGRGHSAAIHSRNEANIMAFAAALP
                     ALRMAVNAGCSLGASGYETNLGPSMTIGTGFAGGSSLSENLTPQHLMQYSRIAYNKDA
                     SEAFGRFTGLDPLNLPKSAKPAASLVVNNPETDELRRELRKIILEELNAVLAA"
     gene            complement(3384..>4298)
                     /locus_tag="PKV23_02360"
     CDS             complement(3384..>4298)
                     /locus_tag="PKV23_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013894129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HQX84306.1"
                     /translation="EAVDIAIKSARYATKRRKIVSIIKGYHGHSGLAVATGDDRFTKI
                     FLSDQPETFIQVPFNDIAAMETALKGNDCAALIMETIPATYGFPMPKDGYLNACKALC
                     EKYGAMYIADEVQTGLMRTGSMWGWQAYGIQPDIMVTAKGLSGGLYPISACLVNEKTG
                     EWLNEDGAAHISTSGGAELGCIVAHKVIEMLLRKETIANVDMVTEFFREGMKEMMDRH
                     GDIFTGVRQKGVILGLEFNHPEGAVSVSRALYEHGVWAIFSSLDKRVLQWKPGVLLSA
                     GLCQEILDRFDAAMPRARELLRSADKVN"
BASE COUNT          839 a         1263 c         1263 g          933 t
ORIGIN      
        1 gccagccgcc agcaagcgct ccttgtcgct cccgtggatc tccagaatgc cgaattgtcg
       61 ctccacgaac aggatgcccg gctccaggtc aggatcggcc ttgagggcca gatcaatcac
      121 ccgctcgatc atcaggccgg gagcgacctc gatgataagc gcatgctggc ccgcataggg
      181 cgggtagccg cgggcccgtg tgggcgtgcc cagataggcg gcaaactgcg gctgcaggct
      241 ttcaagcgtg agaaagaccc gcaagtcgcc cttgccgggc gtgcgatcag gaaccacgtt
      301 tcaggccttc tttatttcgc cgtgcctgag gtgaaatgct tcgatacatc cgcatgcgga
      361 cgcggaatca catgaactga aaccagcgtg ccaacctggc cggcaacttc agcgcctgct
      421 tcggtggcgg ccttgaccgc gccaacatcg ccgttgatga tcacggcaac caggccatcg
      481 cccacttcct cgcggccgat gatcgtgacg tttgcggcct tcaccatggc atcagccgcg
      541 gcaagggcag ccacgtagcc tttggtctca accattccaa ttgcattgct cgacatcttc
      601 ttctcctgtg gtttatgtgc ttcgttactt cttcttttcc gaggactcat cgatggagcc
      661 gataatcagc gcatcgatgg gcggtgcctt gcctggaaac catgctgcgg caacggagcc
      721 cgttgccaca atgacttttt cgccatcgcc gcatcccagc gggtcgaagg cgaggagcct
      781 tgtcgacgaa tcctccacct ggacctcaag aatggatccg gttggaacgt cgccaagctt
      841 ctttgtggac cagatgcggc ccgtgaccgt tgccttgagc atcagcgtct cctttcaatt
      901 ttgaggccaa gtttgcgcgc cctgtcgcgt gccagcggcg tgagcacggc ggtgggaccc
      961 agtatgacgg cgccttttcc tgcgagggcg gaaatttttt gttccgagat gacaccttcc
     1021 aggatgtcac ccgatcccgt tggggcagga gcagtgtgcc ccagcgtgaa tcgcagtttt
     1081 ccggcgcgaa ctgattcaat aacaccggga ctcgatatac gggcaacaaa ggcctgcagg
     1141 tctgcatcgc tgccaatgcg gatcgtttcg gcaccctgtt ttggagctgc ccgattggcc
     1201 attgcttcct tgagcacttc ccgcaccagt ccgcgcaatt cctgtgaagt ttcagctttc
     1261 ataattccag ggcctgttct gcggcgccgg ccgcggcttt cacttctgat tcggtgcctg
     1321 aaaggtaaag cctgcccgtg gcgccgatca tgcgataatc gacgatcttg atattggcgg
     1381 ccttctccgc cgcgtttgac gcgaggatgg cgtaggccgc gggctcagtc tccagaacga
     1441 acagcgtttc accggccaga atcatcgagc cccccttgtt gcggttgatc agaaatgcat
     1501 gattggggtc gattctggtg atgatttttg aagcaagaat tttgggcggc atggcatcgg
     1561 aagaattttt gcccaacgcg gcaagcaccg cttcggcgga ggccttgact gccgcgatgt
     1621 cgcgggaatg gatttcaaga tagccgaatt gccgctccac caccagaatg ccgccttgca
     1681 catcggcctc tttcagggcg atgtcggtta aggcttcgat atcaagtccc ggcgccacct
     1741 cgatgatctg cgccgccata ttgcggcggg gcagcgatcc tttgatccat gagccgagat
     1801 agcacatggt ctgcggctgc agctggtcga ggaaaatgaa tgagcgcagt attgccatgg
     1861 attaagccgc gagaaccgcg ttcagctcct cgaggatgat tttgcggagc tcgcggcgca
     1921 gctcgtcggt ttccggattg ttgacgacaa gcgaggctgc gggtttcgcg gactttggca
     1981 ggttcagcgg atcaagcccc gtgaagcggc cgaacgcctc gctggcatcc ttgttatagg
     2041 cgatgcggga atactgcatg agatgctgcg gtgtgaggtt ttcgctgagc gacgagccgc
     2101 cggcaaagcc tgtgccaatg gtcatggagg ggccgagatt ggtttcatag ccggaagcgc
     2161 caagcgagca gccggcattg acggccatgc gaagcgcggg cagggcggcg gcgaaagcca
     2221 tgatgtttgc ctcgtttctg gaatggattg cggcggaatg gccgcgcccc gaattgcgca
     2281 tcatcgagcg cgccgccgaa atggcctggt caatattgcc gacccgcgca aaggcgagga
     2341 ccgggcagag cttttcccgc accagttttt cttccggctg cacgagatct accggcgtca
     2401 ccagaattct ggcattgggg gccgggaagc ccgccgcctt ggcgataaca ctggcgtccc
     2461 tgccgatcat gcccacgttg aagccaattt cggtgaacag aagttgcctc agtttctggg
     2521 cttcttcggc cgtgcaaata tgcgcgcctt ctgccttcat cgaagcaagg agggactccg
     2581 caacgctgga aaaggccaga accgcggatt cgttggtgca gagaatggaa ttgtcaaagg
     2641 ccttggatgc gacgatccgc ttggccgcaa gcctcatgtc cgctgtgtca tcaaccagca
     2701 cgggggcatt gccaggcccc acgccgtaag cagggtttcc tgaacgatag gcagccttca
     2761 ccaccgacgg cccgccggtg gcgacgacca aatcaatgcg ttcgtcggcg atcagctttt
     2821 caatgagcgg aatgttgggt tcttcaagca cctgaataac gccgtcgggc gcgccagcgg
     2881 ccttcgccgc ctccgccagc atgcgggcag catctgcgca gcatgccttg gctccgggat
     2941 gggggctcag gatgatggca ttacgcgtca tcagcgccag gagtgttttg aaataaaccg
     3001 tggccaccgg gttggtgaca ggaataagcg cgaagatcac gcccgccgga cgtggcagct
     3061 cgacgatctt cttggcctcg tcaatgcgcg gggaaacaaa attttcaccg gaataggttt
     3121 cggcaagccc cttcgaacag gcttcattct tcatccgctt gtgcgccacg acacccatgc
     3181 cggtttcgcg cactgcccat tcagcataat gctgcgcctt gccatgggct gccgcggcca
     3241 ccgcatttac aatgcgctgg gtcgtgctgc gatcaagttt ttgaaatctt gtggcgcccc
     3301 agcgggcgcg gtcgagcatc atccgtgcct gctcaagggc ggcatcgggc actttcaaca
     3361 aagcttcact catggcaggg tcctcaattc actttgtctg cactgcgtag cagttcgcgc
     3421 gcccgcggca tggctgcatc gaaccggtca agaatttcct ggcataagcc ggcgctcagc
     3481 agaacgccgg gtttccattg caacacgcgc ttgtcgaggg aggaaaagat cgcccacaca
     3541 ccatgttcat aaagtgcccg ggagactgat acggcgcctt ccggatgatt gaattcaagg
     3601 ccgaggatca cgcctttctg gcgcacgccg gtgaagatgt cgccatggcg gtccatcatc
     3661 tccttcatgc cttcgcggaa aaattctgtg accatatcga cattggcaat ggtctctttc
     3721 cgcaaaagca tttcgataac cttgtgagcc acgatgcagc cgagttcggc gccgcccgag
     3781 gtgctgatat gggcagcgcc atcctcattc agccactcgc cggttttctc gttcaccagg
     3841 cacgcgctga tcgggtagag cccgcctgat aaccccttgg ccgtaaccat gatatcgggt
     3901 tggattccat aggcctgcca gccccacatg gaacctgtgc gcatcaggcc ggtttgcact
     3961 tcgtcggcga tatacatggc gccatatttt tcgcacaacg ccttgcaggc attgagatag
     4021 ccgtctttcg gcatcgggaa accataggtt gcgggaatgg tttccatgat cagcgccgcg
     4081 caatcattgc ccttgagcgc tgtttccatg gcggcaatgt cgttgaaagg aacctggata
     4141 aaggtctccg gctggtcgct gagaaagatc ttggtgaaac ggtcatcacc cgtggcaacc
     4201 gcaagacccg aatggccgtg atagcccttg atgatggaga ctatcttgcg tcgtttcgtt
     4261 gcatagcgag cgctcttgat ggcgatgtcc acggcctc
//
LOCUS       DAORVN010000077         4286 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_15546_length_4286_cov_2.870243, whole genome shotgun sequence.
ACCESSION   DAORVN010000077 DAORVN010000000
VERSION     DAORVN010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 4286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4286
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15546_length_4286_cov_2.870243"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(190..681)
                     /gene="rpsI"
                     /locus_tag="PKV23_02365"
     CDS             complement(190..681)
                     /gene="rpsI"
                     /locus_tag="PKV23_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015318209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HQX84307.1"
                     /translation="MSEQTTLADLGAAMGMAAAANPAAPVAPAKPKIDAKGRAYATGR
                     RKNAIARVWIKRGSGKVTVNGKVIEAYFARPVLRMLVQQPMIAVNRNTQMDVECTVVG
                     GGLSGQAGAVRHGISRALTHFEPDLRPALKKGGFLTRDSRAVERKKYGKAKARRSFQF
                     SKR"
     gene            complement(687..1151)
                     /gene="rplM"
                     /locus_tag="PKV23_02370"
     CDS             complement(687..1151)
                     /gene="rplM"
                     /locus_tag="PKV23_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011580503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HQX84308.1"
                     /translation="MSTYSAKAKDIVKNWVLIDAEGLVVGRLATIIANRLRGKHKPTF
                     TPSMDCGDNIIVINCEKIAFSGAKRKDKTYYWHTGFPGGIKERKAHQILDGKFPERVL
                     EAAVKRMLPGASLKRQQMTNLRIYSGSAHPHEAQNPVKLDIKAMNPKNVLRG"
     gene            1471..2121
                     /locus_tag="PKV23_02375"
     CDS             1471..2121
                     /locus_tag="PKV23_02375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX84309.1"
                     /translation="MASAQADANSPDGHFIDQGMWPDLAVSDSHGDDTQPTPPADVED
                     LTEPDPTQPGDGETPPEDEVSIGEIPIVQMVELTPETAQKAVDAYVIVKEKYKDAALE
                     NFDSLQDFVDRDPLGKAFEADIKAEGFANVTEWNLAITTVGAAYSNIIDDQSTDIKQQ
                     IEEVENDTELAQDMKDRIITSLKALIPSENNHKVVQGLIDNPVYAEKLKTLETEEE"
     gene            2118..2900
                     /locus_tag="PKV23_02380"
     CDS             2118..2900
                     /locus_tag="PKV23_02380"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009027887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HQX84310.1"
                     /translation="MSDLILREESDGILRLTLNDPATRNSLSEKMIEALHDAISTAKA
                     RVIILSAHGPAFSSGHNLKEITARRGDADEGRRFFKRLFDKCADLMLAITNSPCPVIA
                     EVDGLASAAGCQLVASCDLAIATDRATFCTPGVNIGLFCSTPMVALTRAVTRKHAMEM
                     LLTGDVIPASEALRIGLVNRVTTHNELKAVTQELAQKIASKSGAAIKVSKQAFDRQAD
                     MPVAEAYAYMAQVMADNLLLSDAHEGIGAFIQKRTPAWDQDH"
     gene            2897..3322
                     /locus_tag="PKV23_02385"
     CDS             2897..3322
                     /locus_tag="PKV23_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HQX84311.1"
                     /translation="MKPRINFLTLGVTDMVAMRAFYERLGLTASSASNPDVTFFDANG
                     IVLALFGYHALAKDAGVSAGPVPAFRGVGVAWNAGSENEVDRIMAQAKSAGGTIKKKA
                     EKVFWGGYSGYFADPEGHLWEVAYNPFMPIDAEGRVQLP"
     gene            3319..3858
                     /locus_tag="PKV23_02390"
     CDS             3319..3858
                     /locus_tag="PKV23_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007197000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HQX84312.1"
                     /translation="MNHDSYPADYIRGILHSVKVIAMVGASSNVVRPSYLVMQYLLSK
                     GYDVIPVNPGLAGRELQGKMVYGTLKDIPQAIDMVDIFRNSDAAGAIVDEALTLTPLP
                     SVIWMQLSVRDDAAAMRAEAAGVKVVMNRCPKMEYGKQSGEWAWVGGNSGILSSRRQN
                     MHESGKMQSLGLGVIPTKR"
     gene            3888..>4286
                     /locus_tag="PKV23_02395"
     CDS             3888..>4286
                     /locus_tag="PKV23_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003597343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HQX84313.1"
                     /translation="MTDQPPGFDTLAIHAGAAPDPSTGARVTPIYQTTAYVFNDVKHA
                     SDLFALRAFGNIYTRIMNPTQGVLEAKIAALEGGTAALALASGHAAQMLVFHTIMQPG
                     DEFVAVRQLYGGSINQFNHSFKSFGWHVVWA"
BASE COUNT          914 a         1203 c         1271 g          898 t
ORIGIN      
        1 gagggggact cgcaatttgt ccgctgctac ctatttctag tggcagaagc gagcctgttt
       61 ttcccctccc ccgcaatggc gggcttgctt ggaaggcccg gtctcccctg cccgggcttt
      121 cctttttcct ccttggaaaa gaaaaaggcc ggagttttct ccggcctttt ttgcggggtg
      181 aaagttcgct tagcgcttcg agaactggaa gctgcggcgg gccttggcct tgccgtattt
      241 cttgcgttca acggcacggc tgtcgcgagt caggaagcca ccctttttca gggccggacg
      301 gaggtccggt tcaaaatggg tgagggcacg cgaaatgccg tggcgcacgg cgccggcctg
      361 gccagagagg ccgccgccga caacggtgca ttcaacatcc atctgggtat tgcggttaac
      421 tgcaatcatc ggctgctgaa ccagcatccg cagaaccggg cgggcaaagt aggcttcgat
      481 gactttgcca ttcaccgtaa ccttgccgct gccgcgcttg atccagacgc gggcaatggc
      541 gtttttgcgg cggccggttg cataggcccg gcccttggca tcgatctttg gcttggcagg
      601 ggcaacgggc gcagcaggat ttgccgcagc cgccattccc atggcagcgc ccaggtccgc
      661 gagagtcgtt tgttctgaca tgacgcttag cctctcaata cgttcttggg gttcattgct
      721 ttgatatcga gcttgaccgg gttctgggcc tcatggggat gggccgaacc gctgtaaatc
      781 ctaagattgg tcatctgctg gcgcttgagg gaagcaccgg gaagcatgcg cttcacggcc
      841 gcttcaagaa cccgctcggg aaacttgcca tcgagaatct ggtgcgcctt gcgctccttg
      901 atgccgccgg ggaagccggt gtgccagtaa taggtcttgt ccttgcgctt ggcaccgctg
      961 aaggcgatct tttcgcaatt gatgacgatg atgttgtcgc cacagtccat cgagggggtg
     1021 aaggtgggct tgtgcttgcc gcgcaggcga ttggcaatga tcgtggccag gcggccgaca
     1081 accagtcctt ctgcgtcgat cagcacccaa ttcttcacga tgtccttggc tttcgccgaa
     1141 taggtgctca tgtttttttc tctgggttaa tgatcacaaa tgaaaaggcc acaggaacac
     1201 tgcagccgtt tcggttgcgc ggtgattacg gtagacatgg gacgaagtca agaattaccc
     1261 cagaaaaacg tgaatttcgt tgatgtggta atatattacc tcagaactat tgttttcctc
     1321 caaatcttcg gcgaatgctg ctaaagatag gcagcggatg tcgctgttaa gatgggaaaa
     1381 gacctccgac cgggaagaat aatgacgcgt ttggctgggt ttttaggcgc tttctgggcc
     1441 gcattcgcgc taacgggaat gtgtccagcg atggcttccg cccaagccga tgcgaactcc
     1501 cccgatggac atttcatcga ccagggcatg tggcccgatc tggcggtgag cgacagtcat
     1561 ggcgatgaca cgcagccaac ccctcctgcc gacgttgagg accttacgga accggacccc
     1621 acccagcctg gcgatggcga aacgccgcct gaagacgaag tgagcattgg cgagatcccg
     1681 attgtccaga tggtggaact gaccccggaa accgcgcaaa aagccgtaga tgcctatgtg
     1741 attgtcaaag agaaatacaa ggatgcggca ctcgagaact tcgacagcct tcaggatttt
     1801 gttgaccgcg acccgctcgg caaggccttt gaggccgata tcaaggccga gggctttgcc
     1861 aatgtgacag aatggaacct tgccatcacc acagtgggtg ctgcttactc caacatcatc
     1921 gacgatcagt cgaccgacat caaacagcag attgaggaag tcgagaatga cacggaactg
     1981 gcgcaggata tgaaagaccg gattatcacg tcgctcaagg ctctgatccc gtcggagaac
     2041 aatcacaaag tcgtgcaggg cttgatcgac aatccggtct atgctgaaaa actgaaaacg
     2101 ctggaaaccg aagaagaatg agcgatctga tcctgcgcga ggagagcgat ggcattttgc
     2161 gcctgacgct caatgatccc gccacacgca acagtctttc cgaaaaaatg atcgaggcgc
     2221 tgcatgacgc catcagcacg gccaaggcgc gcgtcatcat cctgtctgcc catggtccgg
     2281 ccttcagttc cgggcacaac ctgaaggaaa tcaccgcgcg ccgcggcgac gcggatgaag
     2341 gccgccgttt cttcaagcga ctgttcgaca aatgcgccga cctgatgctt gccatcacga
     2401 acagcccctg ccctgtcatt gcggaagtcg atggactggc aagtgctgcg ggatgccaac
     2461 tggtggcgag ctgcgacctg gcaattgcga cggaccgggc gacattctgc acgcccggag
     2521 tcaacatcgg gctattctgc tcgacgccga tggtcgccct tacccgcgcc gtcacacgca
     2581 agcatgccat ggaaatgctg ctgacgggcg atgtcattcc tgcttccgaa gcgcttcgca
     2641 taggtctggt caatcgtgtc accacgcaca atgaactcaa agcggtgacc caagaactgg
     2701 cacagaagat tgcctcgaaa tccggtgctg cgatcaaggt tagcaagcag gcgtttgacc
     2761 ggcaagccga tatgccggtg gcggaggcct acgcctacat ggcgcaggtg atggctgaca
     2821 atctgcttct gtctgacgcc catgaaggca tcggggcctt tatccagaag cgaacgccgg
     2881 cgtgggacca ggaccattga agccacgcat aaactttctg acgcttggcg ttaccgacat
     2941 ggttgccatg cgggccttct atgaacggct tgggctcact gcatcgagcg ccagcaatcc
     3001 ggatgtgacg ttctttgatg ccaatggaat tgtgctggcc ctgttcggct atcacgcact
     3061 ggcgaaggac gcaggcgtca gcgcgggccc ggttccggct tttcgcgggg ttggcgtggc
     3121 gtggaatgcg ggcagcgaaa atgaggttga ccgcatcatg gcccaggcaa aatcggcagg
     3181 cggtacaatt aagaagaaag cggagaaggt tttctggggc ggctattccg gctatttcgc
     3241 cgacccggag ggccacctgt gggaagtggc gtataacccc ttcatgccga tcgacgcaga
     3301 agggcgcgtt caactgccat gaatcatgac agctatccgg cagattacat ccgtggaatc
     3361 ctgcacagcg tcaaggtgat cgccatggtg ggcgcctcca gcaacgtggt gcgcccttcc
     3421 tatctggtga tgcaatatct cctgtccaaa ggctatgatg tcattccggt gaatcccgga
     3481 ctggcgggtc gggaactcca gggcaaaatg gtttatggga cgctgaagga cattccccag
     3541 gcaattgaca tggttgacat cttccggaat tcggacgctg ccggggccat tgtcgatgag
     3601 gctttgactt taactcccct gccctcggtt atctggatgc agctatcggt ccgcgatgac
     3661 gccgccgcga tgcgcgcgga agccgccggg gttaaagttg tcatgaaccg ctgccccaag
     3721 atggaatacg gcaagcaatc gggtgaatgg gcctgggtgg gcggcaattc gggcattctg
     3781 tcctcacgca ggcagaacat gcacgagagc ggaaaaatgc aaagcttggg acttggcgtc
     3841 atccccacca agcgataggc tcacccctta aagcacagga gaatagcatg actgaccagc
     3901 caccgggatt cgatacccta gccatccacg ccggcgcggc accggatccc tccacgggtg
     3961 cacgggttac gcccatctac cagaccacgg cctatgtctt caacgatgtg aagcatgcct
     4021 cggatttgtt cgcgctgcgg gccttcggca acatctatac ccgcatcatg aacccgacgc
     4081 agggcgttct tgaagccaag atcgcggccc tggaaggcgg cactgcagcc ctagcgctgg
     4141 cctcgggcca tgcggcgcag atgctggtgt tccacaccat catgcagccg ggcgatgagt
     4201 tcgtggcggt gcgccagctc tatggcggat cgatcaacca gtttaatcac tcattcaagt
     4261 cattcggctg gcatgtggtc tgggcc
//
LOCUS       DAORVN010000078         4262 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_15676_length_4262_cov_3.321131, whole genome shotgun sequence.
ACCESSION   DAORVN010000078 DAORVN010000000
VERSION     DAORVN010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 4262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4262
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15676_length_4262_cov_3.321131"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            265..1206
                     /locus_tag="PKV23_02400"
     CDS             265..1206
                     /locus_tag="PKV23_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003567609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HQX84314.1"
                     /translation="MKKFILAAAMTALMSGTALAANIGVSMALFDDNFLTVLRNGMVA
                     QAKGMEGVNLQVEDAQNDVAKQLDQIKNFIASGVDAIIVNPVDTSATQAMSDAAAAAN
                     VPLVYVNREPINVDTLPDNQAFVASNEKDSGTLETQEVCRLFKEMGKTEAKVYVIMGE
                     LSNQAAVMRTKDIDDVIARPECSFIKIIDKQTSNWRRDEAQNLMTNWLSTGTAFDGVI
                     ANNDESAIGAIQAMKAANVDMKSVVVGGVDATQDALVAMQAGDLDATVFQDAAGQGAG
                     ALDAALKLSKGEAVDQKVYIPFQLVTPANIGDFLKKN"
     gene            1336..2412
                     /locus_tag="PKV23_02405"
     CDS             1336..2412
                     /locus_tag="PKV23_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015314569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQX84315.1"
                     /translation="MAQATHGVGGLKYDASKRARPPELSVFVALMLIVATFEILGRLV
                     IGDSFLFNTRENVAGLFNQQRLEVIILQVSIVGIIAIGVTQVIITGGIDLSSGSVVGA
                     TAMIAMSFAQVAIVNGNPNPKAIFPELGWLDLPVIVPILVGMGCGLIAGLINGSLIAY
                     TRIPPFIATLGMMVAARGLSRWWSTGNPISFPTESYAAIGKGLMPVMIFITLAILFQL
                     IMSYTKYGRHCYAIGSNEEAARVSGIKVEHHKVLVYVIAGVLASVAALVLSSKNLTAQ
                     AGMGVMYELDAIAMTVIGGASLAGGRGSIIGTVIGALIFGVIISGFTFLKLDAYYQEM
                     VKGVIIVGAVVLDQWRQRRQAIGG"
     gene            2416..3186
                     /locus_tag="PKV23_02410"
     CDS             2416..3186
                     /locus_tag="PKV23_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008872818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQX84316.1"
                     /translation="MEEVILRTEGLTKHYGGVHALENANFELRMGEHVAIMGDNGAGK
                     STFVRQITGVEQRTSGKIIFGGQEVQFKGPLDARQAGIETVFQNLALADDLDVPSNLF
                     LGREKIWLNLGPFSILDFKGMRAATEKALLRTAVKIPNLSNSIRKMSGGQRQCVAIAR
                     TATFASKLIIMDEPTAALGVQETTQVENIIRTLKVNGEPLIIVSHNMRQVFDLADRIV
                     VFRRGRIVANLNKSETNGQDVVAYITGAKTGAEYADAA"
     gene            complement(3187..4092)
                     /locus_tag="PKV23_02415"
                     /pseudo
     CDS             complement(3187..4092)
                     /locus_tag="PKV23_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008193721.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT          891 a         1279 c         1262 g          830 t
ORIGIN      
        1 tcgcatgcaa ccccaagagg agtgtttgac tctcccggaa agtagaatat tttatccaaa
       61 ttggggctaa gatgttccaa atattccatc ttggttccat cgctccagac tgcccaatca
      121 ataaggcgca gctggcgacc gggaggattt gaatattttc acatcttgtc caaccgtgat
      181 tttttgcgcc tagccccgtc ttggggtcat tccggcaaat caaaaaaggg tgccgccgga
      241 cacccttacg gaggagatac aaacatgaag aaatttattc tcgcggccgc catgacagcg
      301 ctcatgtccg gcaccgcact tgccgcaaat atcggcgtat ccatggccct gttcgatgac
      361 aacttcctga ccgttctgcg caacggcatg gttgcccagg caaaaggcat ggaaggcgtc
      421 aacctgcagg ttgaggatgc acagaatgac gtggccaaac agcttgacca gattaaaaac
      481 ttcatcgctt ccggcgttga tgcgatcatc gtgaacccgg tcgatacttc ggcgacccag
      541 gccatgtcgg acgccgctgc tgccgccaac gttccgctgg tttatgtgaa ccgcgagccg
      601 atcaacgtcg acacgctgcc ggacaaccag gcattcgtcg cctcgaacga aaaggattca
      661 ggcactctgg aaacccagga agtttgccgc cttttcaaag aaatgggcaa gaccgaagcc
      721 aaagtctatg tgatcatggg cgaactctcg aaccaggctg ccgtgatgcg caccaaggat
      781 atcgacgatg ttatcgccag gccggagtgc agcttcatta agatcatcga caagcagaca
      841 tcgaactggc gccgcgacga ggcccagaac ctgatgacca actggctctc gaccggcacg
      901 gcctttgacg gcgtgattgc caacaatgac gaaagcgcca ttggcgccat ccaggccatg
      961 aaggccgcca acgtcgacat gaagtcggtc gtcgtcggcg gcgttgacgc aacccaggat
     1021 gcgcttgtgg ccatgcaggc aggcgatctc gatgcgaccg ttttccagga cgcggcgggc
     1081 cagggggcgg gcgcacttga tgcggcgctc aagctctcga aaggcgaggc ggtggaccag
     1141 aaggtttaca tccccttcca actggtgacg ccggccaaca ttggcgactt cctgaagaag
     1201 aattgagtcg cattgcacta cggcaactgg gagcggcgca tcggcgccgc tccttttcat
     1261 aatgcggcat cttgctctaa tgtaacttga agctgggagg tgggcgcaag gcgcgcccgt
     1321 cagtggagga taaaggtggc acaagcaacc catggtgttg gcggcctgaa atatgatgcc
     1381 tcgaagcggg cgcggccgcc cgaactcagc gtatttgtgg cgcttatgct catcgtcgcg
     1441 acttttgaaa tcctgggccg cctcgtcatc ggcgacagtt tcctgttcaa cacccgtgaa
     1501 aacgtcgcag ggcttttcaa tcagcagcgg ctggaagtga tcattctcca ggtttcgatc
     1561 gtcggcatca tcgcgatcgg cgtcactcag gtgatcatca ccggcggcat cgacctgtcc
     1621 tccggctccg ttgtcggagc caccgccatg atcgcaatgt cttttgcgca ggttgccatc
     1681 gtcaacggca accccaatcc gaaagcgatt tttccggagc tgggctggct cgacctgccg
     1741 gtcatcgtgc cgatcctggt ggggatgggt tgcgggctca ttgccggcct catcaacggg
     1801 tcgttgatcg catatacgcg catcccgccc ttcatcgcta cgctcggcat gatggtggcg
     1861 gcgcgcggcc tgtcgcgctg gtggtcaacc ggcaatccta tttccttccc caccgaaagc
     1921 tacgccgcaa taggcaaggg gctgatgccc gtcatgattt tcataacgct tgccatccta
     1981 ttccagctca tcatgagcta cacgaaatac ggcaggcact gctatgccat aggctccaac
     2041 gaggaggccg cgcgcgtctc cggcatcaag gtcgagcatc acaaggttct tgtctatgtg
     2101 attgccggag tcctggcctc agtggccgcg ctggtgctga gctccaagaa tctcacggcg
     2161 caggccggca tgggcgtgat gtatgagctt gacgccattg ccatgacggt gatcggcggc
     2221 gcgtcactcg ccggcgggcg tggctcgatc atcggcacgg tcatcggagc cttgattttc
     2281 ggcgtgatca tttcgggctt caccttcctg aagcttgatg cctattacca ggagatggtg
     2341 aaaggcgtga tcatcgtggg cgccgtggtg ctggatcaat ggcggcagcg ccgccaggca
     2401 atagggggct gagccatgga agaggttatt ctgcgcaccg aagggctgac caagcactat
     2461 ggcggcgtcc acgccctgga gaacgcgaac tttgaactgc gcatgggaga acatgtcgcc
     2521 atcatgggtg acaatggcgc gggcaagtca acctttgtcc ggcagatcac cggcgtcgaa
     2581 cagcggacca gcggaaagat cattttcggc ggccaggaag tgcaattcaa gggcccgctt
     2641 gatgcccgcc aggcggggat cgagacggtg ttccagaacc tggcgctggc cgacgatctc
     2701 gatgttccct caaacctgtt tctcggtcgg gaaaaaatct ggttgaatct cgggccgttt
     2761 tcgatcctgg atttcaaggg aatgcgtgcc gcgaccgaga aagccctgct gcgcaccgcc
     2821 gtcaaaatcc caaacctcag caactcaatc cgcaaaatgt cgggcggcca gaggcaatgc
     2881 gtggcgattg cccgcaccgc aacctttgct tcgaaactca tcatcatgga tgaacccacg
     2941 gcggcactcg gtgtccagga aacaacccag gtcgaaaaca tcatccgcac cttgaaggtc
     3001 aacggcgaac ccctgatcat cgtctcccat aacatgcgcc aggttttcga cctggccgac
     3061 cggatcgtcg ttttccgccg cgggcggatc gtagcgaacc tcaacaagag tgaaactaac
     3121 ggccaggacg tggtggccta tatcaccggc gccaagaccg gagcggaata cgccgacgcg
     3181 gcgtgatcag gctgcagcct ggaatgccgc cagtttctgg cggtagctca gcgcttcggc
     3241 gatgtggagc cgcgcgatct gctcgcggcc atcgagatcg gcaagggtgc gggccacgcg
     3301 caagacccgg tgatagcccc gcgccgacag gcccatggca tcggctgcat cgcgcatcag
     3361 cttgagacca gcagaatccg gctgggcgat ttcttcaagc agcgcgccat cggcctgggc
     3421 attgatgcgc aagtgagcca tgcccagttt ttcaaagcgc tgcttctgcc tgagccgcgc
     3481 cgcggcaaca cggacggcaa catcggcgct gctttccttg ggcggcggca gggtgagatc
     3541 ggcaatgcgc accgccggca attcgatctg gatatccatg cggtccagca atggcccgga
     3601 aatccgcgcc tgataatcag tggcacagcg cgggcctttc ttgcacacat gccccggctc
     3661 gcccgccatg ccgcacttgc acgggttcat cgcggccacg aactggaatt gcgccggata
     3721 ggtgacgtga taattggccc gcgccaccac cgtctcgccc gtttccagag gttgccgcaa
     3781 tgactccaaa acgcgcggct ggaattcggg aagctcgtcg agaaacagca cgccgtgatg
     3841 ggccagcgcc atttcgccgg gcttggcgcg cagcccgccg ccgacaaggg ctggcatcga
     3901 tgccgaatga tgcggtgaac ggaagggccg ccgccgcgac agccttccat ctttcaactc
     3961 accgccaagc gaggccacca tgctgacatc gagcatttca cgcgggtcca tcggcggcag
     4021 gacggagggc aggcgctgcg ccagcatgga cttgcccgca cccggtggcc ccaccatcag
     4081 catggtgtgt ccaattaatc ctactgagga ccagccaggt tcaatggggc acaccatcaa
     4141 catgttctcg atgatcggac tggtgctgct ggttggcctg gtggccaaga attcgatcct
     4201 gctggtcgac ctcaccaacc agcggcgcgc ccagggtctg gcgatcgatg cggcgctgac
     4261 ga
//
LOCUS       DAORVN010000079         4227 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_15896_length_4227_cov_3.174497, whole genome shotgun sequence.
ACCESSION   DAORVN010000079 DAORVN010000000
VERSION     DAORVN010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 4227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4227
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15896_length_4227_cov_3.174497"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..268)
                     /locus_tag="PKV23_02420"
     CDS             complement(<1..268)
                     /locus_tag="PKV23_02420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQX84317.1"
                     /translation="MIIRELTSSDYDAAVPRLAEILVDAVDSGAGVTFMHPLPQDVAE
                     AYWMKQKADVASGQTMVFVADETGVIGGTVILQKVWPPNQPHRCE"
     gene            complement(265..885)
                     /locus_tag="PKV23_02425"
     CDS             complement(265..885)
                     /locus_tag="PKV23_02425"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HQX84318.1"
                     /translation="MSRTLVLVRHGQSEWNLKNWFTGWKDPDLSEQGVREAHAAGKRL
                     KAKGYVFDVCFTSVLTRAQHTLKIMLEELGQTGLPETRDQALNERDYGELTGLNKDDA
                     RKRWGEEQVLIWRRSYAVPPPGGESLKDTLARALPYYMIHIQPLVLSRKRVLVSAHGN
                     SLRALIMAIEGLTPEQILKRELETGVPVCYRIGADSRPISVETLSA"
     gene            complement(885..1667)
                     /gene="dapB"
                     /locus_tag="PKV23_02430"
     CDS             complement(885..1667)
                     /gene="dapB"
                     /locus_tag="PKV23_02430"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HQX84319.1"
                     /translation="MKLAIAGAGGRMGRVLARIIDETPGCSVAGGLEPKGSALVGSAM
                     GLTGMTISDDPAALFRKIDGIIDFTLPAATLSLVEFSAKAGIVHVIGTTGIDAAGDEK
                     IRAAGKSARIVKSGNMSLGVNILASLVRKAAQALGEEFDIEVLEMHHRHKIDAPSGTA
                     LLLGKAAAEGRKIDLAKRSLRSRDGHTGARPAGDIGFAALRGGSVVGDHTVIFAGNSE
                     RIEFSHKAESRDIFAHGAVKAALWAKGQKPGLYSMTDVLGLE"
     gene            1752..2507
                     /locus_tag="PKV23_02435"
     CDS             1752..2507
                     /locus_tag="PKV23_02435"
                     /inference="COORDINATES: protein motif:HMM:NF023984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3750 domain-containing protein"
                     /protein_id="HQX84320.1"
                     /translation="MRRAFKVLVWFVVLTMVVPSALGAAIGYARGWPASWRQASWASS
                     GTLPDAATTNTAKVMVLSSRTGRWKGIFAEHMSIVMKLAGDTSWTRYDVVGWGMPVRE
                     NAYPADAFWYGNSPRVIHELEGEAAERLIPAIRASIRKYPYSASGSYLIWPGPNSNTF
                     VSWVVRHTDGFAAELSPVAVGKDYLGPGLQFARAPSETGYTVSLNGYVGATLAYAEGF
                     ELHIAGGTIGIDPDDLSIKLPAFGKLSVWDLAG"
     gene            complement(2617..2904)
                     /locus_tag="PKV23_02440"
     CDS             complement(2617..2904)
                     /locus_tag="PKV23_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014330759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HQX84321.1"
                     /translation="MAKAYWIAHVTVTDPDQYKHYAGDAPIAFKKYGAVFLARGGASE
                     QMEGNGRPRNVVVEFPSLQAARDCYNSAEYQAARAKRVGAGEADIVIVEGV"
     gene            complement(2908..4071)
                     /gene="dnaJ"
                     /locus_tag="PKV23_02445"
     CDS             complement(2908..4071)
                     /gene="dnaJ"
                     /locus_tag="PKV23_02445"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006935395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HQX84322.1"
                     /translation="MAKRDFYEVLGLQKGADEKALKAAYRNHAKKFHPDANPGDKAAE
                     AKFKEISEAYDILKDPQKKAAYDRFGHQAFENGAGGGHRPGGAGFGPEFNSSMSDIFE
                     DLFGDFMGGGGQRTSQRGRGGGGAARGADLRYNMEISLTEAYSGKTAQIRVPTSVSCD
                     TCKGSGAKPGTSPKTCTTCKGAGAVRSQSGFFTVERACPTCQGRGQVISDPCSACAGQ
                     GRVTKERNLSVNIPQGVEDGTRIRLANEGEAGLRGGPTGDLYIFLTVRPHEFFQRDGA
                     DLFCKVPISMAVATLGGEIEVPTIDGKKARVTIAEGAQTGKQFRLKGKGMPVLRSSQA
                     GDMYIQVNVETPVNLSRRQREILKEFSEEARSNSPESEGFFAKAKAFWDGFGS"
BASE COUNT          735 a         1266 c         1344 g          882 t
ORIGIN      
        1 cttcgcagcg gtggggctgg ttgggcggcc agaccttctg caaaatgacg gtgccgccga
       61 tcacgccggt ttcatcggcg acgaagacca ttgtctggcc ggaagccacg tccgccttct
      121 gcttcatcca gtaagcttcc gccacatcct gcggcaaggg atgcatgaaa gtgacaccgg
      181 cgccggaatc caccgcatcg accaggattt ctgccagcct cggcacggcg gcgtcatagt
      241 ccgaacttgt cagttcacgg atgatcatgc agacagtgtt tccaccgaaa tgggccgcga
      301 gtcagcgccg atcctgtagc aaaccggaac gccggtctcc aattcgcgct tgaggatctg
      361 ctccggcgtc aggccttcga tcgccatgat gagggcgcgg agcgaattgc catgggctga
      421 gacgaggacg cgcttgcggg aaagcaccag cggctggata tggatcatgt aatagggaag
      481 ggcgcgggcc agcgtgtcct tcaggctttc gccgccgggc ggcggcacag cgtatgagcg
      541 gcgccaaatc agcacctgct cctcgcccca tctcttgcgg gcgtcgtcct tgttgaggcc
      601 ggtgagttcg ccatagtcgc gttcattgag tgcctggtcg cgggtttccg gcaggccggt
      661 ctggcccagt tcctccagca tgattttcag ggtgtgctgg gcccgggtga gcacgctggt
      721 gaaacacacg tcaaaaacat agcccttggc cttcagccgc tttcccgccg catgcgcttc
      781 cctgacgccc tgctccgaga gatcgggatc cttccagccg gtgaaccagt tctttaaatt
      841 ccattcgctt tggccgtgac gcaccagcac gagggttctg ctcatcattc caatcctaaa
      901 acatcggtca ttgaatagag gccgggcttc tggcctttcg cccagagggc cgccttgacc
      961 gcgccatggg cgaagatatc gcggctctcg gccttgtggg aaaattcgat gcgctcggaa
     1021 ttgccggcga aaatcacggt gtgatcacca acgacggaac cgccgcgcag ggccgcaaag
     1081 ccgatgtcgc ctgcgggcct tgcccctgtg tgtccatcgc gcgaacgcag ggaccttttg
     1141 gccaaatcaa tcttgcggcc ttcggcagcg gccttgccca gaagaagagc cgtgccggag
     1201 ggcgcatcga tcttgtggcg gtggtgcatt tccagcacct cgatgtcgaa ttcctcgccc
     1261 agcgcctggg ctgccttgcg cacgagggag gcgaggatgt tcacgccgag actcatgttg
     1321 cccgacttca cgatgcgcgc cgattttccg gcggcacgga ttttttcatc gcccgccgca
     1381 tcaatgcctg tcgtgccgat gacatggacg atgccggcct tcgccgaaaa ctccaccagc
     1441 gaaagcgttg ccgccggcag ggtgaaatca atgatgccgt cgattttcct gaacaacgcg
     1501 gcgggatcat cggaaattgt catgccagtc aagcccatcg cgctgcccac cagcgccgag
     1561 cccttgggct caagcccgcc cgccacgctg cagcccgggg tttcatcgat gatgcgggcc
     1621 agcacccgcc ccatgcgccc gccagcgccc gctattgcca gtttcattgt catgaggatg
     1681 tgtttagcaa cgggattgcg gtgagaacaa gtgcctcaat tgcgccatag gattttccca
     1741 gatttgccga ggtgcggcgc gctttcaaag tcctggtctg gtttgtcgtg ctgacgatgg
     1801 ttgtgccatc ggctttgggt gcggccattg gctatgccag aggctggccc gcctcctggc
     1861 gccaggccag ctgggcctcg tcggggacct tgcctgacgc cgcaacaaca aacaccgcga
     1921 aggttatggt cctgtcgagc cggacggggc gatggaaggg catcttcgcc gagcacatgt
     1981 cgatcgtcat gaagctggca ggggatactt cgtggacgcg atatgatgtg gtgggctggg
     2041 gcatgccggt gcgcgagaac gcctatccgg cggacgcctt ttggtacggc aacagccccc
     2101 gggtgattca tgagcttgaa ggcgaggcgg cggagcggct cattccggcg atcagggcca
     2161 gcatccggaa atatccctac agcgcttcag gaagttatct tatctggccg ggaccgaatt
     2221 cgaatacttt cgtgtcctgg gtcgtgcgcc atacggatgg ctttgcggcc gagctttcgc
     2281 cggttgcggt gggcaaggat tatctgggtc ccggattgca gttcgcccgg gcgccgagcg
     2341 aaacggggta tacggtttcg ctcaacggat atgtgggggc cacgctggcc tacgcagaag
     2401 ggttcgaact ccacattgcc ggcggcacca tcggcattga ccccgatgac ctctcgatca
     2461 aactgccggc gtttgggaag ctcagtgtgt gggatttggc gggataacaa aaagcactgt
     2521 catcccgacg aaagtcggga tccacgcttt cagcatgtct acagattgca agtctgggtc
     2581 ccggcgtgcg ccgggatgac agaatagcga taagcgttaa acgccttcga caatcacgat
     2641 atcagcttct ccggcaccca cccgtttggc gcgggcggcc tggtattcag cggagttgta
     2701 gcaatcgcgg gcggcctgca gggaggggaa ttcgaccacc acattgcgcg ggcgaccgtt
     2761 gccttccatc tgttcggatg cgccgccgcg ggccagaaaa accgcgccat actttttgaa
     2821 cgctattggc gcgtcaccgg cgtagtgctt gtactggtcc gggtcggtca cggtgacatg
     2881 ggcgatccaa taggccttgg ccatcgttca gctcccgaac ccgtcccaga aggccttggc
     2941 cttggcgaag aagccttcgg attcgggcga gttgctgcgg gcttcctcgc tgaattcctt
     3001 caggatctcg cgctggcggc gcgagaggtt gaccggggtc tcgacattca cctggatata
     3061 catgtcgccg gcctgggagg agcgcagcac cggcatgccc ttgcccttca ggcggaactg
     3121 cttgccggtt tgcgcgcctt cggcaatggt gacgcgagcc ttcttgccat caatggtggg
     3181 cacttcgatt tcgccgccga gggttgccac cgccatggag atcggcacct tgcagaagag
     3241 atcagcgcca tcccgctgga aaaattcatg ggggcgcacg gtgaggaaaa tatagagatc
     3301 gccggtgggg ccgccgcgca ggccggcttc gccctcattg gcaagccgga tgcgggtgcc
     3361 gtcctccacg ccttgcggaa tattgaccga gaggttgcgt tccttggtga cgcggccctg
     3421 gccggcgcag gcgctgcagg gatcagagat aacctgccct cggccctggc aggtggggca
     3481 ggcgcgctcc accgtgaaga agccggattg cgagcgcacc gcgcctgccc ctttgcaggt
     3541 ggtgcaggtt ttcgggctgg tgccgggctt ggcgccggaa cccttgcagg tgtcgcagga
     3601 gacggaggtc ggcacgcgga tttgcgctgt cttgccggaa taggcctcgg tcagggaaat
     3661 ttccatgttg tagcggagat cggcgccgcg ggcagcgccg ccgccgcccc gcccgcgctg
     3721 gctggtgcgc tggccgccgc cacccatgaa gtcgccgaag aggtcttcga agatatccga
     3781 catggaggaa ttgaattcgg ggccgaaacc ggcgccgccg ggcctgtggc cgccgcctgc
     3841 gccattttca aaggcctggt ggccgaagcg gtcgtaggcg gcttttttct gggggtcttt
     3901 caggatgtcg taggcttcgc tgatttcctt gaacttggct tcagcggcct tgtcgccggg
     3961 gttggcgtcg ggatggaatt tcttggcgtg attgcggtat gcggccttga gcgccttttc
     4021 gtcggcgccc ttttgaagcc ccaggacttc gtaaaaatca cgtttggcca ttcagttcca
     4081 cgccctcgaa tcggaaggcc ccccaaccct taaagctggg gggcttatct ctgtttagga
     4141 cgttttcttg tcgtcgtcac cgacttccgt gaagtcggca tcgacgatgt catcttcggc
     4201 atcgtccttt tccatgttcg gcacttc
//
LOCUS       DAORVN010000080         4207 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_16053_length_4207_cov_4.295761, whole genome shotgun sequence.
ACCESSION   DAORVN010000080 DAORVN010000000
VERSION     DAORVN010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 4207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4207
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16053_length_4207_cov_4.295761"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="PKV23_02450"
     CDS             <1..223
                     /locus_tag="PKV23_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006468765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX84323.1"
                     /translation="FGEASGDLSVVASNTPIWGTSFTLAEPAGYPCINIGPWGRDYHH
                     WLERLHAPYAFETLPKVLLAVIDAVSKKG"
     gene            254..604
                     /locus_tag="PKV23_02455"
     CDS             254..604
                     /locus_tag="PKV23_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019857529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HQX84324.1"
                     /translation="MSITRIGAGKRMSEAVIHGGKAYLAGFVADKTVGKSVKEQTADI
                     LAQIDATLKQAGTDKTKILKANIWLTDIGKWAEMNEAWDAWVVAGQTPARATVEAKLA
                     GPGLDVEIMVEAAI"
     gene            800..1126
                     /locus_tag="PKV23_02460"
     CDS             800..1126
                     /locus_tag="PKV23_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020093388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HQX84325.1"
                     /translation="MGLLTLGGFDSGVEAKSASGVASYYKSGRQTANGERFNPNGLTA
                     AHRTLPFGTKVRVTNMRNGKTVVVRINDRGPFIRGRVIDLSLGAARIVGMTTSGVVKV
                     SYSVLN"
     gene            1258..2430
                     /locus_tag="PKV23_02465"
     CDS             1258..2430
                     /locus_tag="PKV23_02465"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HQX84326.1"
                     /translation="MEAKDDTNQRTEIPARKCVLIVDDDPLICDVLASLFANLGYETV
                     TAEDGEAACDRIASSNLVLAVVDLNMPKLDGFGLLRHIRQHPRTVDLPVIISTGYDDR
                     KSIEEAYRLGASSFVTKPINWAQFGYHAQFVIRNGEIERDLRRAEAEAVAASKMKNGL
                     FRILSHELKTPLIALIGLTDVLAEALKDKVEAIEAEQLGHIVGASQRLNRLISDILVL
                     SKSFSGAASLRVSNENMTDLLDDSIIGLEANAKAKGIKIKIARPGKGLTLRCDSQLLR
                     QAIKKLVDNAIKFSPEGGTIEIWAHRKPDNSTVISVRDNGPGISPSKLEECLLPFIQE
                     DMSYGRPADGLGLGLPIAKAIAEAHGGELICQSTLGEGTVAAVWIPNLAVPVTKAA"
     gene            2581..3609
                     /locus_tag="PKV23_02470"
     CDS             2581..3609
                     /locus_tag="PKV23_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018235213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HQX84327.1"
                     /translation="MKVVFTDDHRLQFAQGEISGGEFVTPFERPSRMEYILRELKARK
                     MDNIIPPKPLDMKAVARVHDKGFLKFLESAWREWTKAGYRGEIVPTGFPARSMQQRCP
                     KHIDGKVGYYIHSVETCITAGTWGAACASASVALTAQKLIAGGEKSAFALCRPPGHHA
                     HDDLYGGYCFLNNAAIATQAFRDSGAARVAILDVDFHHGNGTQDIFYERNDVLFCSLH
                     GEPETAYPYFLGFKDETGAGKGEGYNFNYPLPEGCTYDKWSKALGQACGKVKAFSPDV
                     LVVSLGVDTYEKDPISYFKLKSADFRDYGRRIAKLKLPTLFVMEGGYAIEAIGVNTVN
                     VLEGFLDA"
     gene            3602..>4207
                     /locus_tag="PKV23_02475"
     CDS             3602..>4207
                     /locus_tag="PKV23_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013897024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HQX84328.1"
                     /translation="MRDPRYDVLFEPVKIGPVTAKNRFYQVPHCNGGGYRDPTAAAEM
                     RRVKSEGGWGVIFTEQVEIHHTSEITPFIELRIWDEDDMPGLTRMADAIHSNGALAGI
                     ELAYSGTNGPNLYTREVPMGPMHSPILTFTSDPVQARAMDKQDIKNLRHWHKLAYRRA
                     KRAGFDIMCLYGAHGFGILQHFLSRRTNQRSDEYGGSLENRA"
BASE COUNT          894 a         1204 c         1271 g          838 t
ORIGIN      
        1 ctttggcgag gcatcaggcg atctttcggt ggtggcctcc aacacgccga tctggggaac
       61 gagtttcacg ctggctgaac cggcgggtta cccttgcatc aatatcggcc cctgggggcg
      121 ggattatcat cactggctgg aacgccttca tgcgccttac gcatttgaga cgcttcccaa
      181 ggttctgctc gcggtgattg atgcggtgtc caagaaaggc tagagagcct caacccatca
      241 acaggagaga gcaatgagca ttacaagaat cggcgcgggc aagcgcatga gcgaagcggt
      301 cattcatggc ggcaaggcct atctcgccgg atttgtggcg gataaaaccg tcggcaagtc
      361 cgtcaaggaa cagacagccg atatcctggc gcagatcgac gcgacgctga agcaggccgg
      421 caccgacaag acgaaaatcc tgaaagccaa catctggctc acggatatcg gcaaatgggc
      481 tgagatgaac gaggcctggg atgcctgggt tgttgccgga cagacgccgg cgcgcgccac
      541 ggtggaagcc aagcttgccg gccccggcct tgacgtcgag atcatggtag aagctgccat
      601 ctgacgaatt cctgcttaac ttattggaat ggcgtgtttt ttgcagtgac ggcccgcatg
      661 tggaaccttc cgtactgacg gttgttaact attaacaatt ggttagctgg gggtcacatt
      721 gcggccataa aaggccctat gttgcgtcgc agcatgaaag agagggtctg tcatgaagca
      781 tgtggtttta ggcgttttga tgggtttgct taccctgggc ggtttcgatt ccggggtgga
      841 agcaaagtca gccagcggcg ttgcctccta ttacaaatcc ggcaggcaaa ccgccaatgg
      901 tgagcgcttc aatcccaatg ggctgacagc cgcacaccgg accctgcctt tcggcacaaa
      961 ggtccgggtg accaacatgc ggaatggaaa aaccgtcgtg gtgcggatca acgaccgggg
     1021 gccctttatc cgtggccggg tgattgacct gagcctgggt gcggcaagga ttgtggggat
     1081 gaccacctcg ggcgtggtga aagtcagcta ttcggtgctc aactaagcca cttcattact
     1141 aacagattct tgccgtaacc gtttttctta accatgcttg ttgaagaatt gccgcttctt
     1201 acccgccatt aacactccac gctctaatgc ttcttgaaaa atagtgggaa gtgtcacgtg
     1261 gaagccaagg acgataccaa ccagcggacc gaaatcccgg cgcgcaagtg tgttttgatc
     1321 gtagatgacg acccgctcat ttgtgacgtg ctggccagcc tgtttgccaa tctgggctac
     1381 gaaaccgtaa cggccgagga tggcgaggca gcctgtgacc gcatcgcaag ttcaaatctg
     1441 gttctggccg tcgtcgacct gaacatgccc aagctggatg gcttcggcct gctccgccat
     1501 atccggcagc atccgcgcac cgtcgatctg ccggtgatca tttccaccgg ctatgacgac
     1561 cgcaagtcca ttgaagaggc ctaccgcctt ggcgcctcat cctttgtcac caagcccatc
     1621 aactgggcgc agttcggcta tcacgcgcaa ttcgtcatcc gaaacggtga aatcgagcgg
     1681 gacctgcgcc gcgccgaggc cgaagcggtt gccgccagca agatgaaaaa cggcctgttc
     1741 aggatcctga gccacgagct caagacaccg ctcattgccc tcatcggcct gaccgatgtc
     1801 ctggcggaag cgctgaagga caaggtcgag gcgattgaag ccgagcagct cggccacatt
     1861 gtcggcgcct cgcagcgcct caaccgcctg atcagcgata tcctggttct gtccaagtcg
     1921 ttttccggtg ccgcaagcct gcgtgtttcc aatgaaaaca tgaccgacct gctcgatgac
     1981 agcatcatcg ggctggaggc caacgcgaaa gccaagggca tcaagatcaa gatcgcgcgg
     2041 ccaggcaaag gcctgacgct ccgctgcgac agccagctgc tgcgccaggc catcaagaag
     2101 ctggtcgaca acgccatcaa gttttcaccg gaaggcggca cgattgaaat ctgggcccac
     2161 cgcaagcctg acaattccac ggtcatttcg gtgcgtgaca acgggccggg catttcgcct
     2221 tcaaagctgg aggaatgcct gctgccgttc attcaggaag acatgtccta tggccggccc
     2281 gccgacggcc tgggccttgg cctgcccatt gcgaaagcca tcgccgaggc gcatggcggc
     2341 gaactcatct gccagagcac gctcggggaa ggcaccgttg ccgccgtgtg gattcccaac
     2401 ctggctgtcc cggtcaccaa ggccgcctga gccatccaat tctgacatga ttgtggccag
     2461 ccgtcgcata gggcggcgtt tgtaacaatt ccaattctct gacagactgc ctttgtgaag
     2521 caagttgtta tcggcaaagt cgtgcaactt tgccgcaacg tgctttgtgg aggagatatc
     2581 atgaaagttg tttttaccga cgatcaccgg ctgcagttcg cgcaaggcga aatatcgggc
     2641 ggcgaattcg tcacgccctt cgagcggccc tcgcgcatgg aatatatcct gcgcgaactc
     2701 aaggcgcgca agatggacaa tatcattccg cccaagcccc tggacatgaa ggcggtggcg
     2761 cgggtgcacg acaagggttt cctgaaattc ctggaatcgg cctggcgcga atggaccaag
     2821 gcgggctatc gcggcgagat cgtgccgacg ggttttccgg cgcgcagcat gcagcagcgc
     2881 tgccccaagc atatcgacgg caaggtgggc tattacattc attcggtcga gacctgcatc
     2941 acggcgggca cctggggtgc ggcctgcgct tcggcctcgg tggcgctcac ggcgcagaag
     3001 ctgatcgcgg gcggggaaaa atctgccttt gcgctgtgcc ggccgccggg ccaccatgcc
     3061 catgacgatc tttatggcgg ctattgcttt ttgaacaatg cggccatcgc cacccaggcg
     3121 ttccgtgatt caggagcggc gcgggtggcc attcttgacg tggattttca ccacggcaat
     3181 ggcacgcagg acattttcta tgagcgcaat gacgtgctgt tctgctcgct ccatggcgag
     3241 ccggaaacgg cctatcccta tttcctcggc ttcaaggacg agacgggggc ggggaagggc
     3301 gaaggctaca atttcaatta tccgctgccg gagggatgca cctatgacaa gtggagcaag
     3361 gcgctgggcc aggcctgcgg gaaggtcaaa gccttctcgc ctgacgtgct ggtggtttcg
     3421 ctgggcgtgg acacctacga gaaggatccg atcagctatt tcaaactgaa gtcggcggat
     3481 ttccgcgatt atgggcggcg tatcgccaag ctcaaactgc ccacgctatt cgtgatggaa
     3541 ggcggctacg ccattgaagc catcggggtg aacacggtaa atgtgctgga aggcttcctg
     3601 gatgcgtgac ccgcgctatg acgtgctttt cgagccggtg aagatcgggc cggtgacggc
     3661 gaagaaccgg ttttaccagg tgccgcattg caatggcggc ggctaccgcg atccaacggc
     3721 cgcggccgag atgcgccggg tgaaatcgga gggcggctgg ggtgtgatct tcaccgagca
     3781 ggtggaaatc catcatacat cggagatcac gcccttcatc gaactgcgca tctgggacga
     3841 ggacgacatg ccgggtctca caaggatggc cgatgccatc cattccaacg gggcgctggc
     3901 cggcattgaa ctggcctata gcggaaccaa tggccccaat ctttacaccc gcgaagtgcc
     3961 catggggccg atgcacagtc ccatcctgac cttcaccagc gatccggtgc aagcccgcgc
     4021 catggacaag caggatatca agaacctgcg gcactggcac aagctggctt accgcagggc
     4081 gaagcgcgcc ggcttcgaca tcatgtgcct ttacggggcg catggctttg gcattctgca
     4141 gcatttcctg tcgcggcgga ccaaccagcg cagcgacgaa tatggcggct cgctggaaaa
     4201 ccgggcg
//
LOCUS       DAORVN010000081         4202 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_16082_length_4202_cov_3.296841, whole genome shotgun sequence.
ACCESSION   DAORVN010000081 DAORVN010000000
VERSION     DAORVN010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 4202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4202
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16082_length_4202_cov_3.296841"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1128
                     /locus_tag="PKV23_02480"
     CDS             <1..1128
                     /locus_tag="PKV23_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008123535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyltransferase family protein"
                     /protein_id="HQX84329.1"
                     /translation="RPNVLLNHNHAAPTEDGRIVEKWSFRRSNAFRFVGEECRNVMEN
                     VALQDMSAFAKMEVSGPGAREWLDSILANRIPKKMGRIALCHLLTKLGGVRAEFTVYE
                     WAPGRFYLVSAGAYERHDHDTLYKLLPKDGSVKLNPITTRLGVLVLAGPNSRKVLQKL
                     TSTDLSNEAFPWLSGQAISVGHTSCHALRVNFVGELGFEFHHPIEQQVALFDLLMKAG
                     REFGIKPYGIKAMSSLSIEKSYRLVPRELSIEYSAYESGLDRFVHPNKGEFLGRDALV
                     AGREKGLNWNFVTMEVHGVSDADSDTRGSEPIYSKGKLVGRATNGGFGWRVNKSLALG
                     MVRPEYATLDTELEIKILDKTFKASVIAESPYDADNLKLRA"
     gene            1206..2144
                     /locus_tag="PKV23_02485"
     CDS             1206..2144
                     /locus_tag="PKV23_02485"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HQX84330.1"
                     /translation="MATPEQSYGTGVFFVFLATIGWSLSGVFVRIMPGLDGWQLNCWR
                     GYWLAVSLMVYLVAVYGRGAYRKFLDIPRVALFASALFFALGSTLYVSSLTMVSTATV
                     SVIGATSPLFAALLSPWVTGERPGFEAWIAAMLALLGVGVIAWDGLEAGRSTLGLLLS
                     ICVPMAFAGQTLALRRYRNVDMVPSICLGGIMSFFIAGLLGFTAGHAGGGFDVGFREV
                     LLLAAMATLQLAIPLVFYIKGAHSVPAVTLTLIAMLDAVINPMWPWIFVGEKPGEAAF
                     IGGAIIVGAVLISVFGRKFYNSRAAAADLNAVERPA"
     gene            complement(2161..2883)
                     /locus_tag="PKV23_02490"
     CDS             complement(2161..2883)
                     /locus_tag="PKV23_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HQX84331.1"
                     /translation="MKLSDFKALTFDCYGTLIDWESGMIEGLKPLTSRLKNVLSRNGI
                     LEAHARHESSQQIYTPTMKYSELLAVVYKRLAEEWGLAASHDECQTYGYAVKDWPAFP
                     DSVEALAYLKKHYKLVILSNVDNASFAFSNRRLQVVFDAVYTAQDVGSYKPSDRNFDY
                     MIAKLQGLGIAKSEILHTAESLFHDHAPAHKHGLASCWIYRRHNQQGFGATMDPGTTP
                     KYNFRFNSMADLAAAHRQEAAR"
     gene            complement(2895..3668)
                     /gene="gloB"
                     /locus_tag="PKV23_02495"
     CDS             complement(2895..3668)
                     /gene="gloB"
                     /locus_tag="PKV23_02495"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003527442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HQX84332.1"
                     /translation="MPHLDMRQFMCRSDNYGVIIHDSKTGATASIDAPDAEAVEAELR
                     KFGRQLTHIFTTHHHEDHVAGNLRLKELFGCKIIGPKNEAEKIPGIDTAVKGGDTFSW
                     AGREVKVFDCPGHTRGHIAFYMPSEFSLFAGDTVFAMGCGRVNEGSMAEMWHSVNQFR
                     PLPPVTHVYCGHEYTEANCRFALSVEPGNRDLQQRATVVAAQLARGEMTCPSTIGDEL
                     KTNPFMRCDSREIRKNLKLETATDAEVFAELRHQKNSFK"
     gene            3736..>4202
                     /locus_tag="PKV23_02500"
     CDS             3736..>4202
                     /locus_tag="PKV23_02500"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HQX84333.1"
                     /translation="MDVVDLREFYASPLGNATRRLLSAKLRPRLGSLNDACVMGMGFA
                     TPYLDDLPSETKRTLAFMMARQGVIHWPQSGAVKSALVDEFDLPLLESTVDLALVVHG
                     LELTDSPIEMLQEVWRVMAPQGRLVLVVPNRRGLWAAFDTSPFGYGQPFSRTQL"
BASE COUNT          791 a         1153 c         1254 g         1004 t
ORIGIN      
        1 cggccaaatg tgctgctcaa tcacaatcat gcggcaccga cggaagacgg gcgcattgtc
       61 gaaaagtggt cgttccgccg ctccaacgcc ttccgctttg tcggtgaaga gtgccgcaat
      121 gtgatggaga atgtggccct ccaagacatg tcggcctttg ccaagatgga agtatcgggg
      181 ccgggggcgc gggagtggct ggattcaatt cttgccaacc gcattccaaa aaagatgggg
      241 cgcatcgcac tgtgccatct gctcacaaag cttggcggag tgcgcgccga gttcacggtt
      301 tatgagtggg cgccggggcg tttctatctc gtctccgccg gcgcctacga gcgccatgac
      361 catgacacgc tttacaagct gctgccgaag gacgggtcgg tgaagctcaa cccgatcaca
      421 acgcggcttg gcgtgctcgt gctggccgga cccaactcgc ggaaggtttt gcagaagctg
      481 acatcgacgg atctttccaa cgaagctttc ccgtggcttt ccggccaagc catttcggta
      541 ggccacacaa gctgtcatgc actccgcgtc aactttgtgg gcgaactggg cttcgagttc
      601 caccacccta tcgagcaaca ggttgcattg ttcgatctgc tgatgaaagc gggccgcgaa
      661 ttcggcatca agccctatgg catcaaggcc atgtcgtcat tatcaatcga aaagagctac
      721 cgactggtgc cacgcgaact ttccatcgag tattcggctt acgagtcggg tcttgaccgt
      781 ttcgtgcatc ccaacaaggg tgagttcctg ggccgggatg cactggttgc gggccgcgag
      841 aagggcctga actggaattt tgtcaccatg gaagttcatg gcgtcagcga tgcggattcg
      901 gatacgcggg gctcggaacc gatttattcc aagggcaagc tggtgggccg cgctaccaac
      961 ggcggctttg gctggcgggt gaacaagtcg ctggcgctgg gcatggtgcg gcccgagtat
     1021 gcaactctgg atactgaact ggaaatcaaa atcctcgaca aaacgttcaa ggctagcgtt
     1081 atcgcggaaa gtccttatga tgcagacaat ctgaagttgc gcgcctgatt ctctaagccg
     1141 caatgttcac tattggatgt gtgcgtgttg acgttggcga gaattgagcg gcggcggggg
     1201 caatattggc gacacctgaa caaagttacg gcacaggcgt attctttgtg tttcttgcta
     1261 ccattggatg gagcctttcg ggagtttttg tccgcatcat gccggggctc gacggctggc
     1321 agcttaattg ctggcgcggc tactggttgg ccgtttccct catggtttat ctcgtcgccg
     1381 tgtatggccg cggggcctac cggaaatttc tggacatacc gagggtggcg ctgttcgcgt
     1441 cggccctttt ctttgccttg ggatcaacgc tctatgtgtc ttccctcacc atggtcagca
     1501 ccgccaccgt ttcggtcatc ggcgccacct cacccctgtt tgccgcactc ctcagtccct
     1561 gggtgacggg cgaaaggccc ggatttgagg cgtggatcgc agcgatgctg gcactccttg
     1621 gtgtcggtgt catcgcctgg gacgggctcg aggctggccg ttccacattg gggctgctcc
     1681 tttccatttg cgtacccatg gcttttgcgg ggcagactct ggctctgcgc cggtaccgca
     1741 atgtggacat ggtgccgtcg atttgccttg gtggcatcat gtcgtttttc attgcgggat
     1801 tgctggggtt tacggcgggg cacgcgggcg gcggtttcga tgtgggcttc agggaagttc
     1861 tcctgttggc ggccatggcc acgcttcaac ttgccattcc cctggttttc tacatcaagg
     1921 gggcccattc agttcctgcc gtgacactca cgctcatcgc aatgctcgat gcggtcataa
     1981 atccaatgtg gccgtggatt tttgtcggcg agaaaccggg agaggccgcc tttatcgggg
     2041 gcgccataat cgtgggtgcc gtgctgatca gcgttttcgg acgcaaattc tacaattcaa
     2101 gagccgctgc ggctgacctc aacgctgtcg aaagaccagc ttaagcatat ttatcgagac
     2161 tcaacgtgcc gcttcctgcc ggtgtgccgc ggccagatcg gccatgctgt tgaagcggaa
     2221 attatacttg ggggtcgtcc ccggatccat tgtcgcgccg aagccctgct gattgtggcg
     2281 acgatatatc caacatgatg ccagcccgtg tttgtgtgct ggcgcatggt cgtggaacag
     2341 gctttcagcg gtatggagta tttctgactt cgcaatgcca aggccttgca gcttggcaat
     2401 catgtaatcg aaattgcggt cgctcggctt gtaggagccc acgtcctgtg cagtgtagac
     2461 cgcatcgaaa acgacctgca ggcgacggtt gctgaaggcg aaactggcgt tgtcaacgtt
     2521 cgacaggatg acaagtttgt agtgtttctt caggtacgcc agcgcttcga cagaatcagg
     2581 aaaggcaggc caatccttca ccgcgtagcc ataggtttgg cattcgtcat gggacgcagc
     2641 gagcccccac tcttcggcga ggcgcttata caccacggcc aaaagctcgc tatatttcat
     2701 ggtgggagta tagatttgct gtgaagattc atggcgcgca tgggcttcga ggatgccgtt
     2761 gcgtgaaagt acgtttttca gccgcgaggt cagcggtttg agcccttcta tcatgccgct
     2821 ttcccaatcg atcaacgttc catagcaatc gaatgttagg gccttaaagt cactgagctt
     2881 catattccgg ggcctcactt gaaactgttt ttctggtggc gaagctcggc aaacacttcg
     2941 gcgtccgtgg ctgtctccag cttcaggttc ttccggatct cgcggctgtc gcaacgcatg
     3001 aagggattgg tcttcaactc atcgccgatg gttgaagggc aggtcatctc gcccctggcg
     3061 agctgggccg ccacgacggt ggcgcgctgc tgcaggtcgc ggttacccgg ctcgacggag
     3121 agcgcgaagc ggcagttggc ttcggtatat tcgtggccgc aatagacgtg ggtcaccggc
     3181 ggaagcgggc ggaactgatt cacggagtgc cacatctccg ccatgctgcc ctcgttgacg
     3241 cggccgcaac ccatggcaaa aaccgtatca ccggcaaaga gtgaaaattc ggatggcata
     3301 taaaaagcga tgtgaccccg ggtgtgccca gggcaatcaa agactttcac ctcgcgcccg
     3361 gcccagctga acgtgtcgcc accctttacg gccgtatcga tgcctggaat tttctcagcc
     3421 tcgttcttcg gcccgatgat cttgcagcca aagagctcct tgagccgcag gtttccagct
     3481 acatggtctt cgtggtggtg ggtggtaaat atgtgggtga gctgccggcc aaatttcctg
     3541 agttcggcct ccactgcctc ggcatcgggc gcgtcaatcg aggctgtggc ccctgtcttt
     3601 gaatcatgga ttatcacacc gtagttgtcg gagcggcaca tgaattggcg catatcgaga
     3661 tggggcatat gatttactaa ccctgttttt cttcgttgat gacccattag gcgttaaacg
     3721 ctaaaacccg ccgtcatgga tgttgttgat ctgcgtgagt tctacgcgag tcctttggga
     3781 aatgctacac gccgcctgct ttccgccaag ctgcggccgc ggctgggttc gttgaacgat
     3841 gcctgtgtaa tgggcatggg atttgccacg ccctatcttg acgatttacc ttccgaaacc
     3901 aaacgcaccc ttgccttcat gatggcgcgc cagggagtca ttcactggcc gcagtcaggc
     3961 gccgtgaaat cggccctggt tgatgaattt gaccttccgc tgcttgaaag cacggtggat
     4021 ctggccctcg tggttcatgg actggaactt acggacagcc ccatcgagat gctccaggaa
     4081 gtctggcggg tgatggcgcc gcagggccgg ctggttctgg tagtgccaaa ccgccgcggg
     4141 ctgtgggcgg ctttcgacac ctcgcccttt ggctatggcc agcctttttc gcgcacccag
     4201 ct
//
LOCUS       DAORVN010000082         4173 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_16270_length_4173_cov_2.846527, whole genome shotgun sequence.
ACCESSION   DAORVN010000082 DAORVN010000000
VERSION     DAORVN010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 4173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4173
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16270_length_4173_cov_2.846527"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1755
                     /locus_tag="PKV23_02505"
     CDS             <1..1755
                     /locus_tag="PKV23_02505"
                     /inference="COORDINATES: protein motif:HMM:NF013109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HQX84334.1"
                     /translation="PVIGGFLAATGYLLLTGAIGMGLGARVSIWNLAPLLAPATLMLW
                     LPWVLIGGGIAIASRWFRSDLLLPLVLALALVGFYAILLVAGMDLQQAEDASLLLGPF
                     ETSGFLHVLTPSLVLDADPWAILYAAPTIMVVAMLTILGTLLNATGLELALTRDLDLE
                     KDLRATGIANIAGALGGGMTGYQMIPGTILGQRMGLRSVLPGLSAAAGCLLIFIYGAT
                     LLSALPAGLFVALISYLGIDLLITWLWVRRRQFSLTDYAVVLLILAVAATVGFFEALG
                     IGILAAAAMFIVSFARVNVVRLSSTLAGRRSLVERSNAEVNYLMQVGHQAAVFELSGF
                     LFFGTANALVERVHDELARQKELRFLVIDFARVHGLDASAIYSLGKLSRLCMAAGVRL
                     IFSGLPRYQEAHYRAFAGHKDRALFTDTLDDALQSLEAELLSTRPESAPDTASLLLND
                     LLALSERPQFADKFKRVTLNTGDVLLNQGAESREMFVLLSGAMRAEVARQDGDPLVVA
                     RFMPGAPIGEIAFYGSTARIATVLAEEPSVLVKIDADSLVRSGDDHDPATVVHHYLAV
                     NLARRLMNMTRLMRDADF"
     gene            1846..2739
                     /locus_tag="PKV23_02510"
     CDS             1846..2739
                     /locus_tag="PKV23_02510"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HQX84335.1"
                     /translation="MDWAEQNDLVAVRRCARRDQAEQYALVLTAMGIQSWISPEGRIT
                     ALYVAHEDVVRATEELEAYDSENPRPQPAKAWLHPALPRAEVAFAYWALLLFFFAAAR
                     NQAFAFDWVGEGAVQSARMFNGEWWRAVTALCLHTSGAHILGNLVFGTVFLMLLSQVT
                     GAGVATLAMITAGAAGNVLSAMVHAPEYTSIGASTAIFAGIGLLAALQQARRRDHAQA
                     HIRNWVPVAGGLALLGFLGVSGENTDIVAHMTGFASGIAAGLLMARWDRDWTTDRGLQ
                     WWCAGMAVTLVASSWALAALA"
     gene            complement(2760..>4173)
                     /locus_tag="PKV23_02515"
     CDS             complement(2760..>4173)
                     /locus_tag="PKV23_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012331929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQX84336.1"
                     /translation="RFYSDEDRERGEPQKALDLAAEHGHFEKEGWRIRKDGTRFWASV
                     VIDAIRDPSGDLIGYAKITRDISEKRKAEQALEETREALFQAQKMEIVGQLTGGIAHD
                     FNNLLMVIQSSMELLRKRVPDDEKLLSLIDNATEGVKRGTSLTQRMLSFARRQDLDQK
                     PINLHEFIFEMTDLLQRSLGPTIVIEARFPIGLSMVRADANQLESALLNLAVNARDAM
                     PAGGPLTISAREENLAPGNSLRLAPGKYVCLALEDKGEGMTQDTISHATEPFFTTKGI
                     GKGTGLGLSMVQGFAEQSGGRLCLKSVLGQGTTAEIWLPTAEAMEADSALPSSRTFTP
                     PAKPQDLTVLAVDDDILVRMGTVAMLEDLGHTVREANSGEEALNILAEGAKIDLVVTD
                     QAMPRMTGVQLAEAILAEQPNLPIILATGYAELPSGLGSVLPRLSKPFSQEQLAQALN
                     AAIMKRAKGSDSPDVPPAFK"
BASE COUNT          664 a         1356 c         1283 g          870 t
ORIGIN      
        1 ccggtcatcg gcggcttcct cgccgccacc ggctacctgc tgctgaccgg cgccatcggc
       61 atgggcctgg gtgcccgcgt cagcatctgg aacctggctc ccctgctcgc ccctgcgacc
      121 ctgatgctgt ggcttccctg ggtgctgatc ggcggaggca tcgccattgc ttcgcgctgg
      181 ttccgcagtg acctgctcct gccgctggtc ctggccctgg ccctcgtggg cttctatgcc
      241 atcctccttg tcgctggcat ggaccttcag caggccgagg acgcaagcct gctgctcgga
      301 cctttcgaga caagcggctt cctccatgtg ctcacccctt cgctggtcct cgacgccgac
      361 ccctgggcca ttctgtacgc cgcccccacc atcatggtgg tggccatgct gaccattctc
      421 ggcaccctgc tcaacgccac cggcctcgag cttgcgctga cccgcgacct tgaccttgag
      481 aaggacctgc gcgccacggg catcgccaac atcgccggag cgctgggagg cggcatgacc
      541 ggttaccaga tgatcccggg caccattctg ggccagcgca tgggactgcg cagcgtgctg
      601 ccaggactca gcgccgcggc gggctgcctg ctgatcttca tctacggggc aaccctgctc
      661 tcggccctgc cagcgggcct cttcgtcgcc ctgatttcat atctcggcat cgacctgctg
      721 atcacctggc tctgggtccg gcgccggcag ttctcgctca ccgactatgc ggttgtcctg
      781 ctgatcctcg cggtggccgc caccgtgggc ttcttcgagg cgctggggat cggcatcctg
      841 gcggcagcgg caatgttcat cgtctccttc gcccgcgtca acgtggtgcg gctgagctcg
      901 acgctcgccg gccggcgctc gcttgtcgaa cgcagcaacg ccgaggtcaa ctacctcatg
      961 caggtgggcc accaggccgc ggtgtttgaa ctcagcggct tcctgttctt cggcactgcc
     1021 aatgcgctgg tcgagcgcgt ccatgacgaa ctggcgcgcc agaaggaact gcgcttcctg
     1081 gtcatcgact ttgcccgcgt tcatgggctc gatgcttccg caatctattc cctgggcaag
     1141 ctctcgcggc tttgcatggc agcgggcgtc aggctcatct tcagcggact gccgcgctat
     1201 caggaggcgc actatcgcgc cttcgccggc cacaaggacc gggccctgtt caccgacaca
     1261 ctcgacgacg ccctgcaatc gcttgaagcc gagctcctct cgaccaggcc cgagtcagcc
     1321 cccgacacgg cctccctgct gctcaacgat ctcctggcgc tcagcgagcg gccgcagttc
     1381 gccgacaaat tcaagcgcgt gacactgaac acaggcgacg tgctgctgaa ccagggagcg
     1441 gaatcacgtg agatgttcgt gctgctctcg ggcgccatgc gagccgaagt ggcccgtcag
     1501 gatggcgacc cgctggtcgt ggcgcgcttc atgcccggcg cacccatcgg cgaaatcgcc
     1561 ttctatggca gcacggcgcg catcgccacc gtccttgccg aggagccttc agtgctggtg
     1621 aagatcgacg ccgacagcct tgtccgctcg ggcgacgacc acgaccccgc caccgtggtt
     1681 caccattacc tggccgtgaa tctggccagg cggctcatga acatgacacg cctgatgcgc
     1741 gacgccgatt tctgatgggc ggcgtctgcc gtgcgcagcg ctcttgttta gtccgcggac
     1801 accacctatt tcctcttgac tgttggggca acgtgaaaag tcgtcatgga ttgggctgaa
     1861 caaaacgatc tggtggcggt gcggcgttgt gccaggcgtg accaggcaga gcagtatgcc
     1921 ctggttttaa ccgccatggg catccagtca tggatctcgc cggaaggccg gatcacggca
     1981 ctctatgttg cccatgagga tgttgtaagg gccaccgagg aactggaagc ttacgacagc
     2041 gagaaccccc ggccccagcc tgccaaagcc tggctgcatc ctgccctgcc gcgtgccgag
     2101 gtggcattcg cgtactgggc gctgctgctc ttcttttttg ccgctgcgcg caaccaggcg
     2161 ttcgccttcg actgggtcgg tgaaggggcg gttcaatccg cacggatgtt caacggtgaa
     2221 tggtggcgcg ccgtcacggc actctgcctc cacacaagtg gcgctcacat cctgggcaac
     2281 ctcgtgttcg gcacggtctt tctgatgctg ctgtcccagg tcacgggtgc gggtgttgcc
     2341 acccttgcca tgatcaccgc cggtgcggcg ggcaatgtgc tgagtgccat ggttcacgca
     2401 ccggagtaca cctcgattgg cgcctcaacc gcgatttttg ccggcattgg ccttcttgcc
     2461 gccctgcagc aggcgcggcg gcgggatcat gcgcaggcgc acatccgcaa ctgggttccc
     2521 gtggctggcg gattggcgct tctcggcttc ctcggagtca gcggcgagaa cactgacatc
     2581 gttgcgcata tgacgggctt tgcttcaggc attgccgcgg gcctgctgat ggcacggtgg
     2641 gaccgcgact ggaccacgga tcgaggcttg caatggtggt gtgccggcat ggccgttacg
     2701 ctggtggcat cctcctgggc attggcagca cttgcataag gctgttcatg cgccggggat
     2761 catttgaagg ccggcgggac gtcggggctg tcgctgccct ttgctcgctt catgatggcg
     2821 gcgttcaggg cctgggccag ctgttcctgg ctgaagggct tggagagcct gggaaggacc
     2881 gagccaaggc ccgaaggcaa ttccgcatag ccagtggcaa gtatgatggg aagatttggc
     2941 tgctccgcga ggatcgcttc ggcgagctgc accccggtca tgcgcggcat ggcctgatcg
     3001 gtgacgacca ggtcaatttt ggcgccctcg gcgagaatgt tcagcgcctc ctcgccggag
     3061 ttcgcttccc gcaccgtgtg cccgagatct tccagcatgg ccaccgttcc catccggacc
     3121 aggatatcgt catcgacagc aagcaccgtg agatcctgtg gcttggcggg gggtgtgaaa
     3181 gtcctggatg acggaagcgc cgagtccgct tccatggctt cggctgtcgg aagccagatt
     3241 tcagcggtgg tgccctggcc caacacactc ttcaggcaca ggcgccctcc cgactgctcg
     3301 gcaaatccct gaaccattga caggccgagg ccggtgccct tgccaatccc cttggtcgtg
     3361 aaaaagggtt ctgtcgcatg gctgatcgtg tcttgcgtca tgccttcacc cttgtcctcc
     3421 agcgccaggc aaacatattt tcccggagca aggcggaggc tatttcccgg tgcaaggttt
     3481 tcttcacgcg ccgaaatggt cagcggtccg cctgccggca tcgcatcgcg ggcattaacc
     3541 gcaagattga gcagcgccga ttccagctgg ttggcatcgg cgcgaaccat ggaaagccca
     3601 atggggaaac gcgcttcgat cacgatcgtg gggcccagcg accgctgcag gagatcagtc
     3661 atttcaaata tgaactcatg gagattgatg ggcttctgat ccaggtcctg gcgccgggcg
     3721 aaggacagca ttcgctgggt cagggaggtt ccgcgtttca cgccctcggt ggcattgtcg
     3781 atgagcgaaa ggagcttttc gtcgtccggc acgcgcttgc gcaaaagctc catgctgctc
     3841 tgaatgacca tcagcagatt gttgaagtca tgggcgatgc ctcccgtgag ctggccgaca
     3901 atttccatct tctgcgcctg gaacagggcc tcgcgtgttt cttcaagcgc ctgttcggct
     3961 ttccgctttt cggaaatatc ccgcgtgatc ttggcatagc caatcaggtc tccgctgggg
     4021 tcacggatgg cgtcaataac aacgctcgcc caaaacctgg ttccgtcctt gcggatgcgc
     4081 cagccttctt tttcgaaatg cccgtgctcc gcggccaggt cgagagcttt ttgcggttcg
     4141 cccctttccc ggtcttcgtc cgaatagaag cgt
//
LOCUS       DAORVN010000083         4169 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_16296_length_4169_cov_2.757900, whole genome shotgun sequence.
ACCESSION   DAORVN010000083 DAORVN010000000
VERSION     DAORVN010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 4169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4169
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16296_length_4169_cov_2.757900"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            15..365
                     /locus_tag="PKV23_02520"
     CDS             15..365
                     /locus_tag="PKV23_02520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX84337.1"
                     /translation="MTGFAYISFVFALSVIGALLGGLGHFWRAHATMFAEDLGLGEPL
                     DTLTRDNYQFEKHVVGAEWDDAGFWAPDSVRNFIYYVASGFAGPLLLGIGFWGQRSEI
                     VGAICSSLPSVVCP"
     gene            complement(366..686)
                     /locus_tag="PKV23_02525"
     CDS             complement(366..686)
                     /locus_tag="PKV23_02525"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HQX84338.1"
                     /translation="MRKLLAVLFIAVSLTPAAYATSGPGCLIVTNVADDDALNMRSRP
                     SASSRIVDQLVPGQHGIIHLDEPCTPLSRPWPQRWCRVSHYNAEVVTQGWVKARYVRD
                     SDCP"
     gene            950..3274
                     /locus_tag="PKV23_02530"
     CDS             950..3274
                     /locus_tag="PKV23_02530"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQX84339.1"
                     /translation="MEKGELTSGSTWHAAGQCPQFNGSLNMSKVHLYGTQLYPQLEKL
                     TGQAVSWHGCGGLRLATTDEEVNWFKYVYGVSRLAGYECEIIGPGEIKQYHPFLETFG
                     VKAAFRTVTDGHVAPADITNAMAAGARQLGAEIHRRTRVMDIKLLPSGEWNVITDKGN
                     ITCQHVVNSAGSYCDVVGSWTGHNVPIANMLHHYMITEPLKELIDLAPELPVVRDPYS
                     HAYLREETNGVLVGPYETATAHLCWDGQPPAWDFESELVAPELDRLTPWLERATERMP
                     IFGNAGLKSMITGAITHTPDGTYLSGPAPGPKNYWMHCGASIGICQGGGAGKYLAQWM
                     VHGQAEINMREFDPRRFGNWASKDYTAEVSVADYHHMYYCYKPGEQHEVGRGLRRSSL
                     YEKLKAEGAQFAQIFGWERPRWYDTTGKGEAYSFKRSNWFDAVRDEALAVREKVGLMD
                     LSTFSKFEVTGPDAYGFLERICANKIPAKDGGIILGHLLNENGFIESEITVTRLGPEH
                     FYVLSAAVAQLHDKDQLEWRRLATEKVTITDVTDDFGVLVLAGPLAREVLSKCTATDL
                     SNTSFRWLTGKEAEVAGVKKVRLLRVNYVGELGWELHVPMADMPKVFDALTKAGKPYG
                     LKLFGTYAMNSLRMEKSYRGWGSELTTEIDMFEASMERFIRLEKPEFIGKAATLSMHQ
                     RGARMKLVPLEVANTDSDCVGNEPVYSGDRIVGLTTSGGYGHAVRKSLAFAYVDPKMT
                     GVGTGFDVLMFGEKRKASIIGESPWDPANARLKS"
     gene            complement(3271..3573)
                     /locus_tag="PKV23_02535"
     CDS             complement(3271..3573)
                     /locus_tag="PKV23_02535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX84340.1"
                     /translation="MIKFSFVCACVSFLALGATSPAQALGMCGKRADFVKALNDKYQE
                     SGKALAIAGQVNLVEVFASKAGTWTILVTTPEGKTCIIAAGNSWEDLPPSKNLTSL"
     gene            3760..>4169
                     /locus_tag="PKV23_02540"
     CDS             3760..>4169
                     /locus_tag="PKV23_02540"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HQX84341.1"
                     /translation="MPASPATHPNTLFGIALMIAAMIVLPFLDVVAKFLGEQNVPIME
                     IVWARLFFGMVMTAPLVWKMDGARSLIPQRPVVHTIRAIFLVAATALFFWALKFQGIA
                     ETLAIFFVQPLVITMLSPLVLGEKVGMRRWLAVAV"
BASE COUNT          831 a         1202 c         1303 g          833 t
ORIGIN      
        1 gataatcttc gccgatgacc ggttttgcct atatcagttt tgtcttcgcg ctctcggtga
       61 ttggcgcgct gctgggcggc cttggccatt tctggcgggc ccatgcgacg atgttcgcgg
      121 aggacctggg cctgggcgaa ccgctggata cgctgacccg cgacaactac cagtttgaaa
      181 aacatgtggt gggggcggaa tgggacgatg ccgggttctg ggcgccggac agcgtgcgca
      241 acttcatcta ttacgtggcc agcggctttg ccgggccgct gctcctcggc atcggcttct
      301 ggggccagcg cagcgaaatc gtcggcgcga tctgcagcag cctgccttcg gtggtttgcc
      361 cctgattacg ggcagtcgct atccctgaca tagcgggcct tgacccagcc ttgggtcacc
      421 acttccgcat tgtaatgact gacccggcac cagcgctgcg gccaaggcct gctgaggggc
      481 gtacatggct catcgagatg gatgatgccg tgctggcccg gcaccagttg atcgacaata
      541 cgcgatgaag ccgagggccg gctgcgcatg ttcagcgcat cgtcatcggc cacattggtg
      601 acgatcaggc agccggggcc tgaagtggca taggcggcgg gtgtgagaga aacggcgata
      661 aacagaacgg ccaaaagctt gcgcatgaag aattctcctg atttttccac tatcgctgca
      721 cgcacctgaa cccaccatga acccagcggt ggtgaatccc gctcacctca ggcacaagaa
      781 actcgcttgc gtggcgcttg agaacatgag cactcacggt ttacactgtt ttgttactcg
      841 gaggaatcga atgaagtccc atgcgcgggt ggttgtggtc ggtggcggcg taatgggcgt
      901 tggcctgctt tatcacctgg cgctggaggg ctgaagcgac attgcgctca tcgaaaaggg
      961 cgagctgacc tccggttcga cctggcacgc ggcgggccag tgcccgcagt tcaacggctc
     1021 gctcaacatg tccaaggtgc atctatacgg cacccagctt tatccgcagc tcgagaagct
     1081 caccgggcag gcggtgtcct ggcatggctg cgggggcttg cggctcgcca ccacggatga
     1141 agaagtaaac tggttcaagt atgtctacgg cgtgtcgcgc cttgcgggtt acgagtgcga
     1201 gatcatcggc cccggcgaga tcaagcagta ccacccgttc cttgaaacct tcggggtgaa
     1261 agcggctttc cgcacggtga ccgatggcca tgtggcgcca gccgacatca ccaatgccat
     1321 ggcggcgggg gcgcgccagc ttggggctga aatccaccgg cgcacgcggg tgatggacat
     1381 caagctgctg ccgtcgggcg aatggaatgt gatcactgac aagggcaata tcacctgcca
     1441 gcacgtggtg aactccgccg gttcctattg cgacgtggtg ggctcctgga ccgggcacaa
     1501 tgtgcccatc gccaacatgc tgcaccacta catgatcacc gaacccctga aggaactgat
     1561 cgatctggcg ccggaactgc cggtggtgcg tgacccctac tcccacgctt acctgcgtga
     1621 ggaaaccaat ggcgttctgg tgggacccta tgaaaccgca accgcgcatc tctgctggga
     1681 tggccagccg ccagcctggg atttcgagag cgagctggtg gcgcctgagc ttgaccgcct
     1741 cacgccgtgg ctggaacgcg ccacggaacg catgccgatt ttcggcaatg cggggctcaa
     1801 gtccatgatc actggcgcca tcacccacac gccggatggc acctatcttt cgggccctgc
     1861 tcccggcccc aagaattact ggatgcattg cggggcatcc atcggcatct gccagggtgg
     1921 cggggcgggc aagtacctgg cgcagtggat ggtgcatggc caggccgaga tcaacatgcg
     1981 ggaattcgat ccgcggcgct tcggcaactg ggcaagcaag gattacacgg cggaagtttc
     2041 ggtcgccgat taccatcaca tgtattactg ctacaagccg ggcgagcagc atgaggttgg
     2101 ccggggactg cggagatctt cgctctatga gaagctgaaa gcagagggtg cgcaatttgc
     2161 gcagatcttc ggctgggagc ggccgcgctg gtatgacacc acaggcaagg gcgaggccta
     2221 ttccttcaag cgctcgaact ggttcgatgc ggtgcgcgat gaagccctgg cggtgcgcga
     2281 gaaggtgggg ctgatggacc tctcaacctt ctccaagttc gaggtgacgg ggcccgatgc
     2341 gtatgggttc ctcgagcgga tctgcgccaa caaaattcct gcgaaggacg ggggcatcat
     2401 tctcggccat ctgctgaacg agaacggctt catcgaatcg gaaatcaccg tcacgcgcct
     2461 ggggccggag catttctacg tgctttctgc cgccgttgcc caattgcatg acaaagacca
     2521 gttggagtgg cgcaggcttg ccacggagaa agtcacgatt accgacgtca ccgatgattt
     2581 cggcgtgctg gttctggcag ggccgctggc gcgcgaagtt ctcagcaaat gcaccgccac
     2641 agatctttcc aacacgagct tccgctggct tacgggcaag gaagccgaag ttgcgggggt
     2701 aaagaaggtc aggctgctgc gggtgaatta tgtgggcgag ctgggctggg aactgcatgt
     2761 tccgatggcc gacatgccta aagttttcga tgccctgaca aaggccggga agccatacgg
     2821 actgaaatta ttcggcacct acgccatgaa ttcactgcgc atggagaagt cctaccgggg
     2881 ctggggctcg gaactcacca ctgaaatcga catgtttgaa gcctcgatgg agcgcttcat
     2941 ccgcctggag aagccggaat tcattggcaa ggccgccacc ctttccatgc accagcgcgg
     3001 agcacgcatg aaactggtgc ccctggaagt tgccaatact gactccgatt gcgtgggaaa
     3061 tgaaccggta tacagcggcg acaggatcgt gggcctcacc acgtcgggcg ggtacggcca
     3121 tgccgtcagg aagtcactgg catttgccta tgtggacccc aagatgacag gcgttggcac
     3181 cggctttgac gttctcatgt ttggtgagaa acgaaaggca agcatcatcg gcgaatcgcc
     3241 ctgggacccg gcaaatgccc ggcttaaatc ctagagggat gtgaggttct tgctcggcgg
     3301 caggtcttcc caggaattgc ccgcggcgat aatgcaggtt tttccctcgg gcgtggtgac
     3361 cagaatggtc catgtccccg ccttggaggc gaagacttca accagattga cctggccggc
     3421 gatagcaagc gccttgcctg attcctggta tttgtcattc agcgccttga cgaaatcagc
     3481 acgcttgccg cacatgccaa gggcttgcgc cgggctggtc gcgcccaggg cgaggaatga
     3541 tacgcatgca catacaaaac tgaacttaat catagttcct ccagtctggc cgtccgagac
     3601 gccgccggag gtaactcagc cccctgaggt taggcgggtc ccggagcggc tatgccgatt
     3661 tgatcgttca taaacagctc caactacacg taccgaatca tcatgcgccc aagtcgtaaa
     3721 caaattgcga agagcccggc aatgggatag ctagcggcca tgcctgcctc ccctgccacc
     3781 cacccaaata cgctgttcgg cattgccctg atgatcgcgg cgatgatcgt gctgccgttt
     3841 ctcgatgtgg tggccaagtt cctcggcgag cagaatgtgc ccatcatgga aatcgtctgg
     3901 gccaggctct tcttcggcat ggtgatgacc gcgcccctgg tgtggaaaat ggacggggcg
     3961 cgcagcctca tcccgcagcg cccggtagtt cacacaatcc gcgccatatt cctcgtggcg
     4021 gcaacggcgc tgttcttctg ggcgctgaaa ttccagggga tcgccgaaac gctggcgata
     4081 ttctttgtcc agccgctggt catcaccatg ctgtcgccgc tggtgctggg agaaaaagtg
     4141 ggcatgcgtc gctggctggc ggtggctgt
//
LOCUS       DAORVN010000084         4163 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_16338_length_4163_cov_4.244645, whole genome shotgun sequence.
ACCESSION   DAORVN010000084 DAORVN010000000
VERSION     DAORVN010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 4163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4163
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16338_length_4163_cov_4.244645"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..807
                     /locus_tag="PKV23_02545"
     CDS             <1..807
                     /locus_tag="PKV23_02545"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HQX84342.1"
                     /translation="SVAGPMARSAKDLTAMLKLLAGTDGIEARGLSLHLPDLKQKTFK
                     DFRVAVMLSDPVSEVDQPVQDLIAKLAQFLAKKVKKISHEARPGFTTREAMDIFIALL
                     RSATSRRQSKDEFEANEARVENFAADDDSYYAKMTRAYVMPHRSWLMANERRHQMRLL
                     WDRFFDEWDVMICPAAASAAFPHDQTGDRHERTIEVNGKRVPTTDQLFWAGYSCGFYL
                     PSTVAPLGLTPQGLPSGVQIITRQYGDYTSLRFAELLEREYASFVPPPGY"
     gene            808..1248
                     /locus_tag="PKV23_02550"
     CDS             808..1248
                     /locus_tag="PKV23_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013530544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQX84343.1"
                     /translation="MRRAIAADAAIVRDLTRAAYAKWIPLIGREPKPMTANYERAVVE
                     HIIDLYEEDDVPVALVEVIPQATCLLIENIAVQPSQQGKGLGDLLLKHAEMIAHSLHL
                     DELQLYTNAAFASNIAFYARRGFEEFHREPFPAGGVTVHMRKVI"
     gene            complement(1288..1869)
                     /locus_tag="PKV23_02555"
     CDS             complement(1288..1869)
                     /locus_tag="PKV23_02555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX84344.1"
                     /translation="MKNLSITTASIIVSAVTFTSIAHAESQSFANPKVGAQRLDLCLN
                     WGTGCGKPAADAWCVSKGFTTSTGHVVAHDIGDVRPTRLITTGAVCDQQYCDGFSQVT
                     CFKPGAPPAPDMQAYNKPNFNGMRLDLCVDWSVGCGQPAAKKYCQMKGWATAHSYVVA
                     NNIGSYAPTRLIGTGAVCDQNFCDGFQKIVCKN"
     gene            complement(1988..2794)
                     /locus_tag="PKV23_02560"
     CDS             complement(1988..2794)
                     /locus_tag="PKV23_02560"
                     /inference="COORDINATES: protein motif:HMM:NF014215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQX84345.1"
                     /translation="MSLFKFFAVSAIVAYAAVAFGMYVFQRKLQYHPEHKGLTPESVG
                     IIGASIETLTTADGEKLILWHAPARAGMPTILYFQGNAGEIGDRPLRFNYYHSRGFGV
                     AYLSYRGFGGSSGSPSEAGLITDASAGYDWLIARGVEPGRIALLGESLGSGVAVQLAA
                     QREVGALALEAPYTSTAEVAAKIYWWLPVHALMKDQFKSIDFIAAVVAPLLIIHGEED
                     KLVPVEFGKRLFASASQPKELEIVSGLGHDVLFEETTWARETEFFARVIK"
     gene            complement(2791..3414)
                     /locus_tag="PKV23_02565"
     CDS             complement(2791..3414)
                     /locus_tag="PKV23_02565"
                     /inference="COORDINATES: protein motif:HMM:NF028177.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047869 - dimethylpropiothetin
                     dethiomethylase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfonioproprionate lyase family
                     protein"
                     /protein_id="HQX84346.1"
                     /translation="MSDDLYNDARWLVGAIARGLHSRGGPHVDVVLQRLAEQDMTRED
                     FVEPRPQGLPVTRYFADTIAETMMIESELAAAIASLDGHLKWLQSKSYTDEILGAGFS
                     ENYGWCEIVGPQGFFKGDDFILGLLMLGPQRHYKDHYHPAPELYWPLTGPTDWKQGAG
                     SFETKEAGAVIYHAPMVHHATKTSDQPLLAVWSWTKDIHTPARLAEA"
     gene            complement(3451..>4163)
                     /locus_tag="PKV23_02570"
     CDS             complement(3451..>4163)
                     /locus_tag="PKV23_02570"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HQX84347.1"
                     /translation="TGSEVTRVAVFANAAKTKTWAWGDELKPQVAILDPELTVGVPPH
                     ITAATGLDALVHAIEAATNRHRTDGNDLYALKAISLISENLERAVKNPNDIEARGALL
                     LGSCYGGIAIDNCGTALAHNISHAMADLAPIPHGRATGLAMLATMKWVSQGASEAYAR
                     VAKAMSAADPVIAYDRLVRATGMKISLEGDGLDLAKPELLAEHMASPANAPMRKSNLR
                     YPGDAELVELARLVYDLH"
BASE COUNT          831 a         1198 c         1299 g          835 t
ORIGIN      
        1 tccgtggcgg gccccatggc acgctcggcg aaggatctca ccgccatgct gaagctgctg
       61 gcgggcacgg atggcatcga ggcccggggc ctcagcctgc atcttccgga cctcaagcag
      121 aagaccttca aggatttccg cgtggcagtg atgctgagtg acccggtgag cgaggtggac
      181 cagccggtgc aggaccttat cgccaagctg gcgcagttcc tcgccaagaa agtgaagaag
      241 atttcccatg aggcacggcc cggtttcacc acgcgcgaag ccatggacat tttcatcgcg
      301 ctgcttcgct cggccaccag ccgccggcaa agcaaggatg agttcgaggc caacgaggcg
      361 cgggtggaaa acttcgcggc cgacgatgac agctattacg ccaagatgac gcgggcctat
      421 gtgatgccgc accgctcctg gctgatggcc aatgagcgcc ggcaccagat gcgcctgctg
      481 tgggaccgat tctttgatga gtgggatgtg atgatctgcc cggcggcagc gtccgcagcc
      541 ttcccgcacg accagacggg cgaccggcac gagcgcacca ttgaagtcaa cggcaagcgg
      601 gttccgacca ccgatcaatt gttctgggcg ggttactcat gcggtttcta cctgccgtca
      661 acagtggcgc cgctgggact caccccgcag ggcctgccct cgggcgtgca gatcatcacg
      721 cggcagtatg gcgattacac gtcgctgcgc tttgccgaat tgctggaaag ggaatatgcg
      781 agctttgttc cgccgccggg atattgaatg aggcgcgcca ttgcggctga tgcggcgatt
      841 gtccgcgacc tgacgcgggc cgcctacgcg aaatggattc cgctgattgg gcgtgagcca
      901 aaaccgatga cggcaaatta cgagcgggca gtggttgagc acattataga tttatatgag
      961 gaggacgatg tccccgtggc gttggttgaa gtcattccgc aggcaacctg tctcttgatt
     1021 gaaaacatcg ctgttcagcc aagccagcag ggcaagggcc ttggcgacct gcttctgaag
     1081 catgccgaaa tgatcgcgca ctcgcttcat ctcgatgaat tgcagctcta cacaaatgcg
     1141 gccttcgcct cgaacatcgc attctatgcc cgccgcggat ttgaggaatt ccaccgtgag
     1201 ccctttcccg cgggcggcgt tacggttcat atgaggaagg tgatctgacg gtgtcccctc
     1261 ccgtgaagtg ggagaggaca agaagcatca gttcttgcag acgatctttt gaaagccgtc
     1321 gcagaaattc tggtcgcaga cggctcccgt accgatcagg cgggtcggcg cataggaacc
     1381 gatgttgttg gcgacgacat agctgtgtgc cgtagcccag cccttcatct ggcagtattt
     1441 cttggcggcg ggctggccgc acccaacact ccagtcaacg cagaggtcaa ggcgcatgcc
     1501 gttgaaattc ggcttgttgt aagcctgcat gtcaggtgct ggaggtgcgc ccggcttgaa
     1561 gcaagtcacc tgcgagaatc catcgcaata ttgctggtcg cagaccgcgc cagtggtgat
     1621 cagccgggtt ggcctgacat caccgatatc atgagcaacg acgtggccgg ttgaggtggt
     1681 gaagcccttg ctgacgcacc aagcgtcggc agcaggcttg ccgcagccgg tcccccagtt
     1741 caggcaaagg tcaaggcgct gcgcaccgac cttggggttg gcgaaggact ggctttcggc
     1801 atgggcaatg ctggtaaagg tcactgcact gacaataatt gaggcggtgg tgattgatag
     1861 gtttttcatg gcgttcatcc taaatgtgtt ggtcgcactt cacatgatgt aaggagaatc
     1921 actcccgtaa cttgcaccgt gacacagcac agccgaacgc cgcgtgaatc ccccgttcac
     1981 gcccggttca cttgatcact ctggcaaaga attctgtttc gcgggcccag gttgtttcct
     2041 caaacagcac gtcgtggcca aggcctgaga caatttccaa ttcctttggc tggctggctg
     2101 aggcgaacag gcgcttgccg aactcaaccg gaacaagctt atcctcttcg ccgtggatga
     2161 tgagcagcgg ggccaccacg gcggcgatga aatcgatgga tttgaactgg tccttcatca
     2221 gggcgtgcac tgggagccac cagtagatct tggccgccac ttcagcggtc gaggtatagg
     2281 gtgcttcaag cgccagtgcg cccacttcac gctgtgccgc aagctgcacg gcaacgccgc
     2341 tgccgaggga ttcgcccagc agagctatcc ggccgggctc gactcccctg gcgatgagcc
     2401 agtcgtagcc cgcgctcgca tcggtgatca ggcctgcttc gctgggcgat ccgctgctgc
     2461 cgccaaagcc ccggtaggac agataggcca cgccaaagcc gcgggaatga tagtagttga
     2521 agcgcaaggg acggtcgccg atttcgccgg cattgccctg gaaatagagg atggtgggca
     2581 tgcctgccct ggccggggcg tgccagagga tgagcttctc gccgtcggcg gtggtcaaag
     2641 tttcgatact ggcgccgatg atgccgacac tttccggagt caggcccttg tgttccggat
     2701 gatactgcag cttgcgctga aacacataca tgccaaaggc tacggcagcg taagccacga
     2761 tggcagacac ggcgaaaaac ttgaaaaggc tcatgcctca gccagcctgg cgggggtatg
     2821 gatatctttt gtccagctcc atacagccag taacggctga tctgacgtct tggttgcatg
     2881 atggaccatt ggcgcgtgat agatcacggc gcccgcctct ttcgtttcaa agctgccggc
     2941 gccctgcttc caatcggtgg gcccggtcag cggccaatag agttcgggcg ccggatggta
     3001 gtggtccttg tagtggcgct ggggcccgag catcaaaagg ccgagaatga aatcatcgcc
     3061 cttgaagaat ccctgcggcc cgacgatttc gcaccagcca taattctcgc tgaagccggc
     3121 accgaggatt tcatcggtgt aggatttgga ctgcagccat ttgagatggc catcaagcga
     3181 ggctatggcg gcggccagct cggactcgat catcatggtt tcggcgatgg tatcggcgaa
     3241 gtaacgggtg acgggcaaac cttgcgggcg gggctcgaca aaatcctccc gtgtcatgtc
     3301 ctgttcggca aggcgctgca agaccacatc cacgtggggg cctccgcgcg aatgaagccc
     3361 gcgggcaatc gcacccacca gccagcgcgc atcattatag aggtcatcgc tcatggcgcg
     3421 caccatggcg gcgtgagctt tgaaggagtg ttaatgaaga tcgtaaacaa gccgggccaa
     3481 ttctaccaat tccgcatcac caggatatcg caaatttgac ttccgcatag gggcattggc
     3541 ggggctggcc atgtgctcgg ccagcagctc gggcttagcc aaatccaaac catcgccttc
     3601 cagggatatc ttcattccgg tggcgcgcac cagccggtca taggcgatga ccggatctgc
     3661 cgctgacatg gcttttgcca cccgcgcgta ggcctccgac gcgccctggg acacccattt
     3721 catggtggcg agcatggcga ggcccgtggc cctgccatgg gggatggggg cgagatcggc
     3781 catggcatgg gaaatgttgt gggccagcgc cgtgccgcag ttatcaatgg caatgccacc
     3841 ataacaggag cccaggagca gcgcgccgcg ggcttcgatg tcgttcggat ttttgacggc
     3901 gcgttcgaga ttttccgaga tcaatgaaat ggctttcagg gcgtacaaat cattgccgtc
     3961 ggtgcggtgc cggttggtgg ccgcctcaat ggcgtggacc agggcatcga gcccggttgc
     4021 cgcggtgatg tggggcggca cgcccacggt gagttcggga tcgaggatgg ccacctgggg
     4081 tttcagctca tcgccccagg cccaggtctt ggtttttgca gcattggcga ataccgcaac
     4141 ccgcgtgacc tctgacccgg ttc
//
LOCUS       DAORVN010000085         4144 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_16485_length_4144_cov_2.731475, whole genome shotgun sequence.
ACCESSION   DAORVN010000085 DAORVN010000000
VERSION     DAORVN010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 4144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4144
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16485_length_4144_cov_2.731475"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..457)
                     /locus_tag="PKV23_02575"
     CDS             complement(<1..457)
                     /locus_tag="PKV23_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019443403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX84348.1"
                     /translation="MPKRTDIKSILIIGAGPIIIGQACEFDYSGTQACKALKAEGYRI
                     ILINSNPATIMTDPDFADATYIEPITPEVVAKIIEKERPDALLPTMGGQTALNTALTL
                     NKMGILEKFGVELIGDVLQQLGQCATGGRVHGPAEELFGWVESNLEDPAY"
     gene            complement(529..855)
                     /locus_tag="PKV23_02580"
     CDS             complement(529..855)
                     /locus_tag="PKV23_02580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX84349.1"
                     /translation="MFKTIAATLGILLLASTAGQASVRNFFAPAVDGSRIDNCLSGKA
                     ECGKPAADAFCVKEGFSESILFQREAASATRQLGSDTMCEGENCVSFRRIKCIGPAET
                     AGLTQN"
     gene            complement(982..1182)
                     /locus_tag="PKV23_02585"
     CDS             complement(982..1182)
                     /locus_tag="PKV23_02585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX84350.1"
                     /translation="MKTLIATATLTIMFLSAGAFAGNDIKMASGAKPQVSNVTVILKN
                     GQWPVKGQTSFDACSVRRCLDA"
     gene            complement(1498..1698)
                     /locus_tag="PKV23_02590"
     CDS             complement(1498..1698)
                     /locus_tag="PKV23_02590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX84351.1"
                     /translation="MIRYTFFVLTVLGSAATAIAAIAPASDMPSANAKNVTVIHMNQA
                     FPVYGPIVVEQCAVENCSDAKA"
     gene            1843..2397
                     /locus_tag="PKV23_02595"
     CDS             1843..2397
                     /locus_tag="PKV23_02595"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HQX84352.1"
                     /translation="MNSVYETIESELDVLLPLVANGDRKAFGRLYDLTSGKLFAVVRC
                     VLKKPELAEEALQEAFIKVWERAASYEAGSLRPTAWLAAIARNQAIDLKRRFAERMSA
                     RAEELEAADSVPVMPEAELTMDLSRLKKCLKLLPEERQDMVLLAYYQGWTRDELAAQY
                     KRPATTIKTLLRRSLTVLKDCLDG"
     gene            2390..3094
                     /locus_tag="PKV23_02600"
     CDS             2390..3094
                     /locus_tag="PKV23_02600"
                     /inference="COORDINATES: protein motif:HMM:NF021604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HQX84353.1"
                     /translation="MAKPEDRDELAAEFALGSLPPAERQEAEALMARDAEFAGMVAAW
                     ERRLIPLALALEPVAAPPRIRGAVMKAIAGDAVNSETVVTWKRKASAWRLATLGASAI
                     AASLAAFIFVGTPEPQLTDQRYVAVLQAEGPGPAFFASIDLASGSISVRTVGAPPQPG
                     KSYELWAVGAGRDKPQSLGVIDASFRIPASTLGKIDPAALNDTLFAVSLEPEGGSPTG
                     QATGPVLFTGKLVATE"
     gene            3211..3648
                     /locus_tag="PKV23_02605"
     CDS             3211..3648
                     /locus_tag="PKV23_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX84354.1"
                     /translation="MTLPLVGCAAEGARYVPNSIEGAKNTGIIYVYRPLTQLGQRGED
                     PFVSMAHKSYGRMRAGSFVAGTFPEGEVDVTVQQSMLFLVPTIPKTVTVTVVRGSQSY
                     VKVDQIIDVIGVTDGASVSQSIEIEEMPFNVGQAELAQTRQNI"
     gene            3740..>4144
                     /locus_tag="PKV23_02610"
     CDS             3740..>4144
                     /locus_tag="PKV23_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017957519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HQX84355.1"
                     /translation="MAKIDDLKNQRLAALKATAGGPPKRTSNQFFGVGPITDATTDEA
                     ASRLLRFEFEAWLEKNYRKLDDKGRDLGPFTVAEIGRSMHRGYPADKFLADMMREIHR
                     YFECPKTNKMAVGLGGGHSGFTVCLMHMMNATD"
BASE COUNT          834 a         1118 c         1335 g          857 t
ORIGIN      
        1 tgtacgccgg gtcttcgagg ttcgattcga cccagccgaa aagctcctcg gccggcccgt
       61 gaacgcgccc gccggtggca cactggccga gttgttgcag cacatcacca atgagctcga
      121 cgccgaattt ttcgaggatg cccatcttgt tgagggtgag agcggtgttc agcgcggtct
      181 ggccgcccat ggtgggcagc agcgcgtcgg ggcgttcctt ctcgatgatc ttggccacga
      241 cttccggggt gatgggctcg atgtaggtgg cgtcggcgaa gtccgggtcg gtcatgatgg
      301 tggccggatt ggaattgatc aggatgatgc ggtagccctc ggccttgagg gccttgcagg
      361 cctgggtgcc ggaataatca aactcgcagg cctggccaat aatgataggg ccagccccaa
      421 tgatgagaat ggattttata tctgtacgtt ttggcatgtt ttgaggaaac ccgatttggg
      481 gttccctaca caatcacggg gcggattcca agcttttccg gcgagttatc agttttgcgt
      541 caaaccagcg gtttccgccg gtccgatgca cttgattcgc ctgaaactta cgcagttttc
      601 gccctcgcac atggtatcgg agccgagctg gcgggtggcg cttgcggctt cacgctggaa
      661 gaggatggat tcggagaagc cttcctttac gcagaaagcg tcggcggcgg gcttgccgca
      721 ttcggccttg ccgctcaggc agttgtcaat ccgtgaaccg tcgacagcgg gggcaaagaa
      781 attgcgcacc gaagcttgcc cggcggtgga ggccagaaga aggattccaa gggttgcggc
      841 aatggtcttg aacatttcag gctccggatt tgaagaagat gggacaggaa gcagaatgaa
      901 gcggcttgag agggggctgt tgatccactc cacctgcctc ctgtccctca gccctcttgc
      961 gaaggttgag ggtgaaactg gttaggcgtc gaggcaacgc ctgacggaac aggcgtcgaa
     1021 actggtttgg ccctttaccg gccactggcc gttcttgagg atcacggtca cgttgctcac
     1081 ctggggcttg gcaccggatg ccatcttgat gtcgttcccg gcaaaggcgc cggccgacag
     1141 gaacatgatg gtgagggttg cggtggcgat cagggtcttc attttgtgtc tcctccagag
     1201 actgtgtgca gcgttaagaa caaattaccc cttcgctttg gaatggagcc tgaatggccc
     1261 gttcatctgg cgtttagatt cggctcaaaa ccatccctta ggggcggcaa aagaaaaggc
     1321 cccggggatt gccggggcct ttgagttgca agcaggacat ttgcagggga tcgtgcttgg
     1381 ggagggagat ttttggaacc ggggttggtc atgaccacct tacgagaggg gagggatata
     1441 agttgccatt cccgaacccc ggttccgatg cccagcgggt tagactgggc cgttcaatca
     1501 ggccttcgcg tcggagcagt tctcaacggc gcactgctca acaacgatgg ggccgtaaac
     1561 agggaaagcc tggttcatat ggataaccgt gacatttttg gcatttgccg agggcatgtc
     1621 actggcaggg gcgatggcag caattgcagt tgcggccgat ccaaggactg tgagaacaaa
     1681 gaaggtgtag cggatcataa cgtggtttcc tctcgtgtat ttcgatcttg aacccaacat
     1741 accgaggtaa actcgaaccc aagctgaatg ttccgttcat ctggcgttta tcttgggcgg
     1801 gaaacgtgca actatcgggc gaaatcagcc gtaagtgggg ttatgaacag cgtttacgaa
     1861 accatagaga gcgaattgga tgttttgcta cctttggttg caaacggtga cagaaaggct
     1921 ttcggccgtc tttatgacct tacgtctggc aagctgtttg cggtcgtgcg ctgtgttttg
     1981 aagaagccgg aacttgccga agaggccttg caggaagcat ttatcaaggt ctgggaacgg
     2041 gccgcatcct atgaggcggg atcgctgcgg cccacagcct ggcttgcggc cattgcccgc
     2101 aaccaagcaa ttgacctcaa gcgccgattc gccgagcgca tgtcggcgcg ggctgaggaa
     2161 cttgaagccg ccgattcggt gccagtgatg ccggaggcgg aattgaccat ggatctcagc
     2221 cgtctcaaga aatgcctcaa gcttctgccg gaggagcgcc aggacatggt gctgctggcc
     2281 tattatcagg gctggacccg ggatgagctt gcagcccagt acaagcgccc cgccacgacc
     2341 atcaagaccc tgttgcggcg gagtcttaca gtgttgaagg attgcctcga tggctaaacc
     2401 cgaagacagg gatgaactgg cggccgaatt cgcccttggg tcactcccgc ctgcggaacg
     2461 gcaagaggcg gaagccctga tggcgcgtga cgcggaattt gcgggaatgg tggcggcatg
     2521 ggaacgccgg ctcataccgc tggccctggc gcttgaaccg gtggcagcgc cgccgcggat
     2581 ccgtggtgcg gtgatgaagg ccattgccgg ggatgcggtg aattcggaaa ccgtggtcac
     2641 gtggaaacgc aaagcctcag cctggcggct tgccacgtta ggcgcaagcg ccattgccgc
     2701 gtcactggcg gccttcatat tcgtcggcac gccggagccg caactcacgg atcagcgtta
     2761 tgtggcggtg cttcaggcgg aaggtcctgg tccggcgttt tttgcctcca ttgatctggc
     2821 cagcggcagc atcagcgtgc gcacggttgg cgcgccgccg cagcccggga aatcctatga
     2881 gctgtgggcc gtcggcgccg ggcgtgacaa gccgcagtcg ctgggcgtga tcgacgcctc
     2941 gttccgcatt ccggcgagca cgcttggtaa aatcgatccg gcggcgttga acgacacctt
     3001 gtttgccgtg agccttgagc cggaaggcgg ctcgcccaca ggccaggcaa cgggacctgt
     3061 gctgtttacc ggcaagctgg ttgccaccga ataggttatc cacaggggct tgccgcattg
     3121 cggccttaaa ttgtccttct catcacctca gtgattcggg tggggcggtt catgagtggc
     3181 ttgattacgc gtcggtcagt cttgcaggta atgactttgc cgcttgtcgg ctgtgcggcg
     3241 gaaggggcgc ggtatgtgcc caacagcatc gaaggcgcca agaatactgg aatcatctat
     3301 gtgtaccggc cgctgaccca gctgggccag cggggcgaag acccgttcgt gtccatggcg
     3361 cacaagtcct atggccgcat gcgggcgggc tcgtttgtgg cgggaacctt cccggaaggc
     3421 gaggttgatg tgacggtgca acagagcatg ctgtttctcg tgccgacaat tcccaagaca
     3481 gtcacggtca cggtggtgcg cggcagccaa tcctatgtta aggttgacca gatcatcgac
     3541 gtgatcggcg ttaccgatgg cgcttcggtc agccagtcca ttgaaatcga ggaaatgcct
     3601 ttcaatgtgg gccaggcgga actggcgcag acgcggcaaa acatctgaac ggcgcaaacc
     3661 cccgcgatta tgacgcattt ggggagcggc gctggcacga tcacccatag taatgtcccg
     3721 caaatccgga gatgttatga tggcgaagat cgacgatctg aaaaaccagc gcctggcggc
     3781 gctcaaagcc acggcgggcg ggccgcccaa gcgcacctcc aaccagttct tcggcgtggg
     3841 cccgatcact gacgccacca cggatgaagc agcctcacgc ctgctgcgct tcgaattcga
     3901 agcatggctt gaaaagaact accgcaagct ggacgacaag gggcgcgatc tcgggccctt
     3961 caccgtggcg gaaatcggcc ggagcatgca ccggggctat ccggcggaca aattcctcgc
     4021 cgacatgatg cgcgagatcc accgctattt cgagtgtccc aaaaccaaca agatggcggt
     4081 tgggctgggg ggcgggcaca gcggcttcac ggtgtgcctc atgcacatga tgaacgccac
     4141 ggac
//
LOCUS       DAORVN010000086         4137 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_16533_length_4137_cov_3.207496, whole genome shotgun sequence.
ACCESSION   DAORVN010000086 DAORVN010000000
VERSION     DAORVN010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 4137)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4137)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4137
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16533_length_4137_cov_3.207496"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1009)
                     /locus_tag="PKV23_02615"
     CDS             complement(<1..1009)
                     /locus_tag="PKV23_02615"
                     /inference="COORDINATES: protein motif:HMM:TIGR02283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HQX84356.1"
                     /translation="MFKRLLAALLIATPLFTSVAQADAKFEKFIQTLWPQVKAAGISR
                     SLFDAAFAGVTDPDPAVLKLAATQPEFTSTTSAYLAKAVTPIRIDTGQQLKGSDAALL
                     SAIEKKYGVDRHILLGIWGMESNFGKDKGSMSVMRSLATLLYAGRKKKFAREQIIAAF
                     KILKSGTRTPENFTGSWAAAMGHTQFIPTSYLSYAVDWTGDGKRDIWGSREDALASTA
                     NYLAKAGWKSDRPWGWEVSLPAKFNKALIGRSKWRPVSEWVKLGVTPAGGGKFSAPQA
                     DAFMMIPQGIDGPAFLVTRNFLSLMNYNYSHSYAIAVGHLGDRIRGGGPIVGTWPDLD
                     YD"
     gene            complement(1097..2086)
                     /gene="galE"
                     /locus_tag="PKV23_02620"
     CDS             complement(1097..2086)
                     /gene="galE"
                     /locus_tag="PKV23_02620"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013533607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HQX84357.1"
                     /translation="MTVLVTGGAGYIGSHMVLALVDAGEDVVVLDNLSSGFRWSVAPQ
                     AKLVEGDIGDADFLNRLMAEHKFDAVVHFAGSIVVPDSVSDPLGYYLNNTVKSRTLLD
                     SAVKAGIPRFIFSSTAAVYGNPLTRPVFETEAPAPISPYGTSKLMTEWMLRDTHAAHG
                     LDYVALRYFNVAGADPKGRCGQSTPRATHLIKVACQTALGQRQSMDIFGTDYDTPDGT
                     CLRDYIHVSDLIAAHMKALTHLRRGGESGIFNCGYGKGYSVLEVIRAVEKAHGGPVRA
                     NRVARRAGDPVAIIAGADRVRSVLGWEPQYDDLDAIVASALAWENHLLRRNAA"
     gene            2191..3099
                     /gene="galU"
                     /locus_tag="PKV23_02625"
     CDS             2191..3099
                     /gene="galU"
                     /locus_tag="PKV23_02625"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012317069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HQX84358.1"
                     /translation="MQRPIKKAIFPVAGLGTRFLPATKAMPKEMLTVVDRPLIQIAVD
                     EAREAGIEHFIFVTGRNKSVIEDHFDKQYELESTLRKRGKSSELDILEQDLPGAGQTS
                     FTRQQEPLGLGHAIWCARSIVGDEPFAVLLPDMVMHARPGCLSQMIATYNERGGGNVI
                     AVEKVEPDQVHRYGVVGAKDWRGNSFTIDRMVEKPKPKDAPSNLIISGRYILQPEVFA
                     EISAQAPGAGGEIQITDAMIRLMARQPFHGLAYEGTSYDCGDKLGFLAANVVYGLERH
                     DLGPGFRLELQKIIADHGGRLTWKAE"
     gene            complement(3105..>4137)
                     /locus_tag="PKV23_02630"
     CDS             complement(3105..>4137)
                     /locus_tag="PKV23_02630"
                     /inference="COORDINATES: protein motif:HMM:NF021589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HQX84359.1"
                     /translation="SDARSDLGLSVSPSLRYERDWVRHSWRGQATGDYVAYLENDDYN
                     SLQAEVSSAFRLDIRRTTRAEFDASYVLGQESSADSEVPDTAIGNRTDHALAANAALI
                     HDFGGLEGRAKLGIERQIFEDVDLSGGGTEDNSDRNNTTPSLALRLSYTDPPALKPFV
                     ELAYAPRFHDEKFDRNGLRRDSQGINASLGVTLDQGPFWLGELALVYAMRDYEDSALD
                     TNEAIGINGNLTWAPTDLTSVVLTLATGLNESASATSSGSKTWSGRIDAAHELRENLI
                     LLAGFGAEFEKYSGGTDTTLSPNLGIEWQLNPNLAWTAGYDGTWFEAEASGDNYNEQR
                     LMTGIVLRR"
BASE COUNT          852 a         1189 c         1303 g          793 t
ORIGIN      
        1 gatcataatc gagatcgggc caagtgccga caatggggcc gccgccgcgt atccgatcgc
       61 ccaaatggcc cacggcaatg gcataggaat gggagtaatt gtagttcatg agggagagga
      121 aattccgcgt gacgaggaag gccgggccat caatgccctg cgggatcatc atgaatgcat
      181 cggcctgcgg ggcgctgaac ttgccgccgc cagcgggcgt cacgccaagc ttcacccatt
      241 cggaaacggg ccgccacttg ctgcggccaa tcagggcctt gttgaatttt gccggcaggg
      301 aaacttccca gccccagggc ctgtcggatt tccagccggc cttggccagg taattcgcgg
      361 ttgaggccag agcatcttcc ctggaacccc agatgtcgcg cttgccgtcg ccggtccaat
      421 ccaccgcata ggacagatag gaggtgggga tgaactgcgt gtggcccatg gcggcggccc
      481 aggagccggt gaaattttcc ggcgtgcgcg tgccgctctt caaaatcttg aaagcggcga
      541 tgatctgttc gcgggcgaat tttttcttcc gtcccgcata gagaagcgtt gccagggagc
      601 gcatgacact catggagccc ttgtccttgc cgaagttcga ctccatgccc cagatgccca
      661 agagaatgtg gcggtcgacg ccatatttct tttcaatggc cgaaagcagg gcggcgtcgc
      721 tgcctttcaa ctgctgtccc gtatcgatgc ggatgggggt caccgccttg gcgagatagg
      781 cggaggtcgt cgaggtgaat tccggctggg tcgcagccag cttcaggact gcgggatcgg
      841 gatcagtgac gccggcaaag gccgcatcaa agagcgaacg ggaaattcca gccgccttca
      901 cctgcggcca gagcgtctgg atgaattttt caaattttgc atccgcctgg gccaccgagg
      961 tgaacagtgg cgtggcaatg agcagtgccg cgagtagtcg tttgaacata tgaaccgtcc
     1021 ctgatccaac ctggcgattc gccgccagtc catgtcttta gcagggtggg agaaaagctg
     1081 ccaaatgcgg cgtgaatcag gctgcgttgc gccgcaagag atgattttcc caagccagcg
     1141 ccgaggctac aatggcgtca agatcgtcgt attggggctc ccagcccaaa actgacctca
     1201 cccggtcggc gccggcaatg atggcaacgg ggtcgccggc gcgccgtgcc acgcgattgg
     1261 ccctgaccgg gccgccatgg gccttttcca cggcccgtat gacttccaga accgaatagc
     1321 ccttgccata gccgcaattg aagatgccgc tttcgccacc ccggcgcaga tgggtcagcg
     1381 ccttcatgtg ggcggcgatc aggtcgctca catggatata gtcacgcagg caggtgccgt
     1441 cgggggtgtc gtagtcggtg ccgaaaatgt ccatggattg gcgctggcca agtgcggtct
     1501 ggcaggccac cttgatcagg tgggtggcgc ggggggttga ctggccgcag cggcccttgg
     1561 gatcggcacc ggccacattg aaatagcgga gcgccacata atccaggcca tgggcggcat
     1621 gggtgtcgcg cagcatccat tcggtcatga gtttcgaggt gccgtaaggt gaaatcgggg
     1681 cgggcgcctc agtttcgaac acgggccggg tgaggggatt gccatagacg gccgcggttg
     1741 aggagaagat gaagcgcggg atgcccgcct tcacggcact gtcaagaagg gttcgcgatt
     1801 tcaccgtatt gttgagatag tagcccaagg gatcgctgac ggaatccggc acaacgattg
     1861 aaccggcaaa atggacaacc gcatcaaact tgtgttcggc catcaggcgg ttgagaaaat
     1921 ccgcatcgcc aatgtcgcct tcaaccagtt ttgcctgggg ggcgacggac cagcggaaac
     1981 cggaggaaag attgtcgagc accacgacat cctcaccggc atcaacgaga gccaatacca
     2041 tgtggctgcc gatgtagccg gcgccgcccg ttaccaacac tgtcacgatc aaatatccct
     2101 ttacttgtca cggcctatgc tagattgaat tttacgaaaa ctgtacagca gccggtgccc
     2161 ctttcaaagt ctggttaaga gcaggttaac atgcagcgtc cgatcaaaaa ggcaatattc
     2221 cccgtggccg gcttgggcac gcgcttcctg cccgccacca aggcgatgcc caaggaaatg
     2281 ctcacggtgg ttgaccggcc gctcatccag atcgccgttg acgaggcccg cgaagccggc
     2341 atcgagcatt tcatctttgt cacgggccgc aacaagagtg tcatcgagga ccatttcgac
     2401 aagcagtatg aactggaatc aacgctgcgc aagcgcggca agtcaagtga gcttgatatc
     2461 ctcgaacagg atctgccggg tgccgggcag accagcttta cccgccagca ggaacccctg
     2521 ggactgggcc atgccatatg gtgcgcgcgc agcatcgtgg gcgacgaacc cttcgcggta
     2581 ctgctgccgg acatggtgat gcatgcgagg ccgggatgcc tcagccagat gattgccacc
     2641 tacaacgagc ggggcggcgg caatgtcatc gcggttgaaa aggtggagcc tgaccaggtg
     2701 catcgctacg gcgtggtggg cgccaaggac tggcgcggca acagctttac catcgacagg
     2761 atggtggaga agccgaagcc gaaagatgct ccctcgaacc tgatcatttc cgggcgttac
     2821 atcctgcagc cggaggtctt tgcagagatt tccgcccagg cgccgggagc gggcggtgaa
     2881 atccagatca ccgatgcgat gatccgcctg atggcgcgcc agccgtttca cggcctggcc
     2941 tatgaaggca cgtcctatga ttgcggcgac aagctgggat ttctcgccgc caatgtcgtc
     3001 tatggcctgg agcgccatga cctgggcccg ggatttcgcc tggagctgca gaaaatcatt
     3061 gcggaccatg gcggccgcct gacctggaaa gccgagtgaa gccgctagcg gcgcaggaca
     3121 attccggtca tgaggcgctg ctcattataa ttgtcacctg aggcctccgc ctcgaaccat
     3181 gtgccgtcat agccggcggt ccacgccaga ttgggattga gctgccattc gattcccagg
     3241 tttggcgaaa gcgtcgtatc ggtgccgccg gaatattttt caaactccgc gccgaagcct
     3301 gcgagcaaga tcaggttctc gcgcaattca tgggcggcat caatgcggcc cgaccaggtt
     3361 ttcgatcccg aagaggtggc cgaggcggat tcattcaggc ccgttgccag tgtcaacacc
     3421 acggaggtca gatcggtcgg cgcccaggtg agattgccgt tgatgccgat tgcctcattg
     3481 gtatccaggg cggaatcctc gtaatcgcgc atggcataga ccagcgccag ttcgcccagc
     3541 caaaatggcc cttggtcaag agttacaccg aggcttgcat ttatcccttg cgagtcacgg
     3601 cgcaggccat tgcggtcgaa tttttcatca tggaagcgcg gcgcataggc aagctcgacg
     3661 aaaggcttga gggccggtgg atccgtatag gacagccgca aggcaaggga gggcgtggtg
     3721 ttgttgcggt cggaattatc ttcggtcccg ccgccggaca ggtccacgtc ttcaaagatc
     3781 tgccgctcga tgccaagttt cgcgcggccc tcgagcccgc caaaatcgtg aatgagggcg
     3841 gcgttcgccg ccagggcatg gtcggtgcgg ttgccaatgg cggtatcggg cacctcgctg
     3901 tcagccgaac tctcctggcc cagcacatag ctggcatcaa attcggcgcg cgtggtgcgg
     3961 cggatgtcca gccggaaggc gctgctcact tcggcctgca atgaattgta gtcatcattc
     4021 tccagatagg ccacgtaatc gccagttgcc tggccgcgcc aggaatggcg gacccagtct
     4081 ctctcgtagc gcagggaggg agacaccgag aggccgagat cagagcgggc atcgctg
//
LOCUS       DAORVN010000087         4110 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_16743_length_4110_cov_4.169420, whole genome shotgun sequence.
ACCESSION   DAORVN010000087 DAORVN010000000
VERSION     DAORVN010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 4110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4110
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16743_length_4110_cov_4.169420"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..718
                     /locus_tag="PKV23_02635"
     CDS             <1..718
                     /locus_tag="PKV23_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008935313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQX84360.1"
                     /translation="LVLKDLTREQQIERMRSDFVANASHELRTPLASLSGLIETLQGA
                     ARNDEAAREKFLNLMRSQAERMKRLIDDLLSLSRIEMNVHLRPSAKVDLAQVARHVCD
                     LLSSMAQEEKVTLQINLDAKFEVAGEWDELVQVVVNLVENAIKYSEPGKTVNIEGSRE
                     DGFVTLSVIDEGRGIAEEHIPRLTERFYRISVQESRSRGGTGLGLAIVKHILNRHRGR
                     LLVTSQLGHGSRFTIRLPAI"
     gene            834..1871
                     /locus_tag="PKV23_02640"
     CDS             834..1871
                     /locus_tag="PKV23_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HQX84361.1"
                     /translation="MKKVLLAVSALALFAGPALARDQIRIVGSSTVYPFTTTVAENFG
                     KTSGMKTPVVESTGTGGGMKLFCSGVGEDTPDMTNASRAMKKSEFEACAKNGVTEIVE
                     IKVGFDGLTVAASKDAPDMKLTKQQLFMALAKQVPDKDGKLVDNPYKMWSDIDASLPK
                     EKIEILGPPPTSGTRDSFMELAMEVGAGKFESLEALKKSDAKAFEAVWKSMREDGAFV
                     EAGENDNLIVQKLQANPAALGIFGFSFLEENASSIKGLEIDGATPTFESVASGDYKMS
                     RPLFVYVKKQHVGVVPGIAEFVAEYVSDKALGEDGYLGAKGLITLPGAEAEKTRADAL
                     AMNVLTIDGLK"
     gene            1988..3166
                     /gene="pstC"
                     /locus_tag="PKV23_02645"
     CDS             1988..3166
                     /gene="pstC"
                     /locus_tag="PKV23_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008834927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HQX84362.1"
                     /translation="MTSWTLLIIAALTATGFFVGQAKAQGLRNAGTASLHSLPAYHGL
                     LMASMALAAAGITTLAVAAIAGPNSLVMPAAAALLGTGAIAASYRLITRDFRARNRFE
                     FFVMLVLIACSAVAVLTTVGIVFSVLFESLAFFSEVSPIEFLFGTHWAPTSTPPSFGL
                     IPLLAGTLLITFIAIVVAGPLGLLSAIYMAEYSSRKMRGVLKPMLEILAGVPTVVLGF
                     FAALTVAPLVRNAGESLGLNVASESALAAGLVMGMMIVPLISSLSDDIINAVPQALRD
                     GSYAMGATKSETIKRVVLPAALPGIVSAFMLAISRAIGETMIVVMAAGLAANLTFNPL
                     AAVTTITVQIATLLVGDQEFDSAKTLSAFALGFVLFCFTLVLNYIALRIVQRYREQYD
                     "
     gene            3159..>4110
                     /locus_tag="PKV23_02650"
     CDS             3159..>4110
                     /locus_tag="PKV23_02650"
                     /inference="COORDINATES: protein motif:HMM:NF023240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3333 domain-containing protein"
                     /protein_id="HQX84363.1"
                     /translation="MTDTFDKGNAAFIAKRYASERRFKAYGAVALAVSVLFLVYLIVD
                     IGIKAWPSFFEHRVVLEVNLDASKIPADEVLKGDFEGLVRGSLRSLFPRVTTRGDVRS
                     LTSLLSAGAADDLRQDTAANPASIGTTVRAGALLGDDSDLYLKGMLTTTFRTPGTTNL
                     QLAQSGEEFILTGDLSQLAVGQIVRANGGALRITGVEAGLAKAEAILPPATLNSLTPG
                     TWDILSFSRAEADRRIDDRQVVLLEALRDKGVIEKHFAWRFFTSGDSREPELAGLRGA
                     IFGSLLTLIMTLSLSVPLGIAAAVYLEEFAAKNKWTEIIEV"
BASE COUNT          830 a         1207 c         1172 g          901 t
ORIGIN      
        1 gctggtcctc aaggatctga cgcgcgaaca gcagatcgag cggatgcgct cggatttcgt
       61 ggccaatgcc agccatgaac tgcgcacgcc gctggcttcg ctgtccgggc tcatcgaaac
      121 gctccagggt gcggcgcgca atgacgaagc ggcgcgcgaa aaattcctga atctcatgcg
      181 cagccaggct gagcgcatga aacgcctgat cgatgacctg ttgtctctgt cgcggatcga
      241 aatgaacgtc cacctgcgcc cctcagcgaa agttgacttg gcacaagtgg cccgccacgt
      301 ctgcgacctt ctttccagca tggcgcagga agaaaaagtc accctgcaaa tcaatctgga
      361 cgccaagttc gaggtggcgg gcgaatggga tgaactggtg caggtggtgg tcaacctcgt
      421 cgagaacgcg atcaagtatt cggagccggg aaaaaccgtg aatatcgaag gttcccggga
      481 agacggtttt gtcacccttt cggtgatcga tgaagggcgc ggcattgccg aagagcatat
      541 cccgcgcctt acggagcgtt tctaccgcat cagcgtgcag gagagccggt cacgcggcgg
      601 aaccggcctt ggccttgcga ttgtgaagca cattctcaac cggcatcgcg gccgccttct
      661 ggtgacctcc caactgggcc acggcagccg tttcaccatc cgcctccccg ctatctgaaa
      721 acccttatat tacagtatgt taaactgtca taaaagtgtg atataactgt cgcataagca
      781 tcatcaatgg tttctaggtt ccgctctgtt ctactgacaa ggagacatca gagatgaaaa
      841 aagttctatt ggccgtgagt gcacttgccc tcttcgccgg cccggcattg gcccgcgatc
      901 aaatccgtat cgttggttct tcaacggttt atccctttac gacaacggtt gccgaaaact
      961 tcggcaagac ctccggcatg aagacgccgg tggttgaatc gaccggcact ggcgggggca
     1021 tgaagctgtt ctgctcgggc gtcggtgaag acacgccaga catgaccaac gcttcgcgcg
     1081 ccatgaagaa gagcgaattc gaagcttgcg ccaagaatgg cgtgaccgag atcgttgaaa
     1141 tcaaggtggg tttcgacggc ctgaccgttg ctgcatccaa ggatgcaccg gacatgaaat
     1201 tgaccaagca gcagctgttc atggccttgg ccaagcaagt tcctgacaag gacggcaagc
     1261 ttgtggacaa tccctacaag atgtggagcg atatcgacgc ctcgctgccc aaggaaaaga
     1321 tcgagattct gggtccgcct cccacatcgg gtacgcgcga ttccttcatg gagctcgcga
     1381 tggaagtagg cgctggaaag ttcgagtcgc tagaagcgct caagaagtca gacgccaagg
     1441 cctttgaagc cgtatggaag tccatgcgtg aagatggcgc ctttgttgaa gccggcgaga
     1501 acgacaacct catcgtgcag aaattgcagg ccaatccggc ggcacttggc attttcggtt
     1561 tttccttcct cgaggaaaat gccagcagca tcaagggtct cgaaattgac ggtgcaacgc
     1621 ccaccttcga aagcgttgct tccggcgact acaagatgtc gcgcccgctc ttcgtatatg
     1681 tcaagaagca gcatgtaggc gtggttccgg gcattgccga gtttgtggcc gagtatgttt
     1741 cggacaaggc cctgggcgaa gacggctatc ttggggccaa gggccttatc accctgccgg
     1801 gcgctgaagc tgaaaaaacc cgcgctgacg cgctggcgat gaatgttctc acgattgacg
     1861 gtcttaagta attaggacct gattaggcga atggggtgga ccttgcggat gtaccaagcc
     1921 gccccattcg atcataattc gcgcgacaga cgcgattgaa gaagacaaga caggggctaa
     1981 tgagttcatg acttcctgga cgctactgat catcgccgcc ttgacggcca ctggcttttt
     2041 cgttggccag gcaaaggcgc aaggcctgcg caatgccggc accgccagcc tccattcgct
     2101 ccccgcctat cacggcctcc tgatggcttc catggctctt gccgcagccg gcatcacaac
     2161 gcttgccgta gccgccatcg ccggtcccaa ttcactggta atgccggcag cggcggcgct
     2221 cttgggcacc ggggccatag ccgcatcgta ccggctcatc accagggatt tccgggcccg
     2281 caaccgcttt gaattttttg tcatgctggt gctcatcgcc tgttcagcgg tggctgtgct
     2341 gaccaccgtc ggcattgtgt tttccgtcct gtttgaatca ctggccttct tcagcgaagt
     2401 ctcgccaatc gaatttctgt tcggcaccca ttgggcgcca acctcgacgc ctccttcctt
     2461 tgggctcatt ccgcttctcg ccggaaccct tctcatcaca tttattgcca tcgtggttgc
     2521 cgggcccctg gggcttttgt cggcgattta catggccgag tactccagcc ggaaaatgcg
     2581 cggcgtgttg aaacctatgc tggaaatcct tgccggtgtg cctaccgtgg tgctcggctt
     2641 ctttgccgcg ctgacagttg cgccactcgt ccgcaatgcc ggcgagagcc tgggtttgaa
     2701 tgtggcgtcc gaatcggctc tggcggcggg cctcgtcatg ggcatgatga ttgtgccgct
     2761 catctcctcg ctttccgatg acattatcaa cgcggttccg caagcgctgc gcgatggctc
     2821 ctatgcgatg ggcgccacga aatccgaaac catcaagcgg gtggtcctcc ccgccgcatt
     2881 gccaggcatc gtatcggcct tcatgctggc gatttcacga gccattggcg agacaatgat
     2941 cgtggtcatg gccgcgggcc ttgccgcaaa cctgaccttc aatccactcg cggcggtcac
     3001 cacaatcacc gtgcagatag cgaccctgct ggtgggcgac caagagtttg attctgcaaa
     3061 gacgctctcg gcttttgcgc tcggtttcgt gcttttctgc ttcacccttg ttctaaacta
     3121 catcgcactt cgcatcgtgc agagataccg tgaacagtat gactgataca tttgataagg
     3181 gtaacgcggc cttcatcgcc aagcgctatg ccagtgagcg gcggttcaag gcctatggtg
     3241 ccgtggcgct ggctgtttcg gtcctgttcc tggtttatct tatcgtcgac atcggcatca
     3301 aggcgtggcc aagcttcttc gaacaccgcg tggtgctcga ggtaaacctc gatgccagca
     3361 aaatccctgc cgatgaagtg ctgaagggtg attttgaggg cctggtaagg ggcagcttgc
     3421 ggagcctgtt ccctcgcgtg acgacccgag gcgacgtgcg aagcctgaca tcactgctat
     3481 cggcaggtgc tgccgatgat ctccgccagg atacggcggc aaatccggcg tcgattggca
     3541 ccaccgtcag ggccggcgca ttgcttggcg atgattcgga cctgtacctc aagggcatgc
     3601 tcacaacgac gttccggacc ccaggcacaa ccaacctgca actggcacag agcggcgagg
     3661 agttcattct caccggcgac ctctcgcaac tcgccgttgg gcaaattgtt cgcgccaatg
     3721 gcggagcgct cagaattact ggcgtggagg ccggccttgc caaggctgaa gccatcctcc
     3781 cgcccgcaac cctcaatagc ctgacgcccg gcacctggga catcctgtca ttttcccgtg
     3841 cggaggccga tcggcgcatt gatgaccggc aggttgtgtt gcttgaagcc ctgcgtgaca
     3901 agggtgtgat cgaaaagcat ttcgcctggc gattcttcac ctccggggac tcccgcgagc
     3961 cggaacttgc tggcttgcgc ggcgccattt tcggttcgct cctcactctc atcatgacgc
     4021 tttccctgag cgttcccctc ggcatcgcag ccgccgtcta tcttgaggaa ttcgcggcca
     4081 agaacaaatg gacggagatc atcgaggtca
//
LOCUS       DAORVN010000088         4102 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_16815_length_4102_cov_3.640969, whole genome shotgun sequence.
ACCESSION   DAORVN010000088 DAORVN010000000
VERSION     DAORVN010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 4102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4102
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16815_length_4102_cov_3.640969"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="PKV23_02655"
     CDS             <1..298
                     /locus_tag="PKV23_02655"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQX84364.1"
                     /translation="ATSTVLVGILDDRIVASVMVGHDGHRGVIYYLAVDPAFRKRGYG
                     KAAVAAGETWLRERGVWKINLMVRNDNAAAGRFYERLGYEVNPVTSYGKRLDGP"
     gene            503..1321
                     /locus_tag="PKV23_02660"
     CDS             503..1321
                     /locus_tag="PKV23_02660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX84365.1"
                     /translation="MNPLPSKRERDEFDKAFSARLTGEPEKRSGDLRLSDRQVQPAPF
                     FNIAGQFGAKLVQYAKPGVSKKVLAAMADDSDPAVSLANIRKLLVGPTHKLHDAMFEE
                     VVTILEESDREVQQALRALERKWATLSHVTDELVAKSLDSTDQNREQAEFLKQELQKS
                     ADSQQEMLSEMFLVIDAKIGELTKQVELKMEALGQKIEADMREAAESQERRLQDLEAR
                     FMASNRAEIENLESRLHRLERKASTSTDDLNEVFAGGLTSIADRLKALRDNQAN"
     gene            complement(1477..1821)
                     /locus_tag="PKV23_02665"
     CDS             complement(1477..1821)
                     /locus_tag="PKV23_02665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX84366.1"
                     /translation="MVRIIICVIGLLLFPSSSYALTCNQWYDTMSGFIEEGKQIQAKL
                     KSLEKDPPAVCAFRRNTQIPIMRRNLLMIENFYPCAAPTGLAARDTAVSIRHVLQKLV
                     NDTASQCAAAGM"
     gene            1950..2363
                     /locus_tag="PKV23_02670"
     CDS             1950..2363
                     /locus_tag="PKV23_02670"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HQX84367.1"
                     /translation="MPDLQHFPRLGASACVWKAGKVLLVQRAKAPVGLWSLPGGQVER
                     GETALAAAERELLEETGITARLSAFVGLYEIIRQDPPFHYAIACYGGFWTAGEATASS
                     DALAARWEAPDELDSLEFAPQVREAIARAQALLGV"
     gene            2508..2768
                     /locus_tag="PKV23_02675"
     CDS             2508..2768
                     /locus_tag="PKV23_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018182523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX84368.1"
                     /translation="MLKFRTKPVPAAYPAEAKHLALRYILDAWEEAVYEGLEPDCIAT
                     AAIFAALSDMVATYGEEPVAVMCAKLPDRIRGGEFSVAQTKQ"
     gene            complement(2769..3383)
                     /locus_tag="PKV23_02680"
     CDS             complement(2769..3383)
                     /locus_tag="PKV23_02680"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HQX84369.1"
                     /translation="MDLSLIIIGTGIGIAMAAPLGPVNLIVIRTALCHGVKLAFLAGL
                     GAVLADVLIAGIAAYGIQSIAHLILDYAKFLQIAGGLLLVVLGIRTARTHFADSDLAA
                     APHAARFGLTFSLSVTNPGLVLGYLAIFSSLSEHLALGTSPFRPAFVVLGIAMGSALW
                     WLALSFVMGRLKTRLGPSTLDTINRWSGILVAAFGFLLLWKAFD"
     gene            complement(3387..>4102)
                     /locus_tag="PKV23_02685"
     CDS             complement(3387..>4102)
                     /locus_tag="PKV23_02685"
                     /EC_number="1.3.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011501652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acryloyl-CoA reductase"
                     /protein_id="HQX84370.1"
                     /translation="GVGEQHNGGFSEMARVSGDWLVAVPKGWTCADTMAVGVAGYTAM
                     LCVMALEEQGVKPGDGEILVTGAAGGVGTVSIVLLAKLGYKVAASTGRPEESEFLKKL
                     GASTIIDRNEFNVPVKPLAKMRWAGAIDSVGSTTLANVISQINNDGAVAACGLAQGMD
                     LPTSVAPFILRGVKLIGINSVTTPLPRRQQAWNRIARDLDLKALAALTTHVKLEDVPR
                     VAADIVAGQVRGRVVVDIR"
BASE COUNT          914 a         1129 c         1277 g          782 t
ORIGIN      
        1 cgctacatcg acggtgctgg ttggcattct cgatgaccgc attgtggcct cggtgatggt
       61 cggccatgac gggcaccggg gcgtcatcta ctacctcgcg gttgatccgg cgtttcgcaa
      121 gcgcggatat ggcaaggcgg cggttgccgc cggcgaaaca tggctgcgcg agcgcggcgt
      181 gtggaagatc aatctcatgg tgcgtaacga caatgcggcg gcaggccgct tctatgaacg
      241 cctgggctac gaggtcaatc cggtgaccag ttacggcaag cgactcgacg gcccctgagg
      301 gggtgcccgg ggtctgcctt gattcgggca tgatcttgct gtggaaatgg actccgttaa
      361 ggttaataat ttactaacat ccgagcggtc gctggattgt taaggatgtt tcttagatat
      421 taagcattgt taacaatcgg gatttcggcg ggtttcgagc gaagacaagt ggacggcagt
      481 aactgacctc ggggagagcg acgtgaatcc actaccaagc aagcgtgaac gggacgaatt
      541 cgacaaggca ttttccgcaa gactgaccgg cgaacccgag aaaagatctg gtgacctgcg
      601 cctgtccgac aggcaggtcc agccggcgcc gtttttcaat atcgccgggc agttcggcgc
      661 gaaactggtg caatacgcca agccgggggt gagcaagaag gtgcttgcgg cgatggcgga
      721 cgattcggat ccggcagtct cgctggcgaa catccgcaag ctgctggttg gccccacgca
      781 caagcttcac gacgcgatgt tcgaggaggt cgtgaccatc ctcgaggaat cggaccgcga
      841 ggtgcagcag gcgttgcggg cgctggaacg gaaatgggcg accctttccc atgtcaccga
      901 tgaactcgtg gccaaatccc tggacagcac ggaccagaat cgcgagcagg cggaattttt
      961 gaagcaggaa ctgcagaaat ccgcggattc ccagcaggaa atgctctcgg agatgttcct
     1021 ggtgatcgat gcgaaaatcg gcgagctgac caaacaggtc gaacttaaga tggaagcctt
     1081 ggggcaaaag atcgaggccg acatgcgcga ggctgccgaa agccaggagc gccggctgca
     1141 ggacctcgaa gcaaggttca tggccagcaa ccgggcagaa atcgagaatc tcgaaagccg
     1201 cctgcaccgt ctcgagcgga aagcgagcac cagcaccgac gacctgaatg aggtttttgc
     1261 cggcggcctt acgagcatcg cggaccggct gaaggcgttg cgggacaacc aggcgaactg
     1321 agtgcaggcg aaagggcttg tagccgggcc ggtgggcccg ggcgccgtaa tcatgctcaa
     1381 aactgtgggc aacactgcag ttcgcggcaa cggcgcgctt gctggccagg aacggttact
     1441 tgggtgccct cttcgctttc agggctgaaa ttctatctac atgcctgcag ctgcgcactg
     1501 ggatgctgtg tcatttacaa gtttttgcag gacgtgccga atgctcacgg cggtgtccct
     1561 ggcagccagc ccggttggag ccgcacaggg ataaaaattc tcaatcatca gcagattgcg
     1621 ccgcataatt ggaatttgcg tattccggcg aaaagcacag acagcaggcg gatccttttc
     1681 caacgatttc agctttgcct gaatctgctt accttcttca ataaaaccgc tcatggtgtc
     1741 gtaccattga ttgcaggtca atgcatatga cgatgaagga aagagaagca atccaattac
     1801 gcaaataatg atacgaacca tgtttgcatt agagcagaat tcgcgcttat tcgcaatctt
     1861 gtccgggggc tatcgtccaa aaaaatcccc gctgctttcc gcagttttcc tgaaactata
     1921 gaggccacac atggagccag cgtaactcaa tgcctgacct tcaacacttc cctaggctcg
     1981 gggcttcggc ctgtgtgtgg aaggccggca aggtcttgct ggttcagcgg gccaaggcgc
     2041 ccgtgggcct gtggagcctg cccggcggcc aagtggagcg gggcgaaacg gcgcttgctg
     2101 cagccgagcg ggagctcctg gaagaaacag gaatcacggc tcgtctcagt gcatttgtcg
     2161 ggctctatga aatcatccgg caggatcccc cgttccacta tgccattgcc tgctatggcg
     2221 ggttttggac ggcgggcgag gccaccgctt cgagtgacgc gctggccgcc cggtgggagg
     2281 cacctgatga actcgattcc cttgagttcg cgccccaggt ccgcgaggcc attgcccggg
     2341 cccaggctct gctaggggtt tgacttaaaa ttcattgttc atattaaccc ttttttaagg
     2401 aacgagtcgc gaaaggccgc gcctttgcta tggtgaatta acgaatgcgg ggcgctggtg
     2461 gaaaagcgct gcgggcgggg atcaatacgt caattgaaaa gactgttatg ttgaaatttc
     2521 ggaccaaacc ggtgcctgcg gcctatccag ccgaggccaa acacctggcg ctacgctaca
     2581 ttctcgatgc ctgggaagaa gccgtctatg aagggcttga acccgattgc attgccacgg
     2641 cggcgatctt cgcggcgctt tctgacatgg ttgccaccta tggcgaagaa ccggttgcgg
     2701 ttatgtgcgc gaaactgccg gaccgcatcc gcggcggcga attctccgtg gcgcagacca
     2761 agcaataatc agtcgaaagc tttccacagg agcaggaagc cgaaggcggc aaccagaatt
     2821 cctgaccagc gattgattgt gtccagcgtg gaaggtccaa gccgggtttt cagccggccc
     2881 atgacgaaac tcagcgccag ccaccacaag gcacttccca tggcaatgcc gaggacaacg
     2941 aacgctggcc ggaatggtga cgtgccaaga gccagatgct cgctcagtga actgaagatg
     3001 gcaagatagc ccagcacaag gccgggattg gtgaccgaaa gcgaaaaggt gaggccgaat
     3061 ctggccgcat ggggtgcggc cgcaagatcg ctgtcggcaa aatgggtgcg cgccgtgcgg
     3121 atgcccagaa cgaccaacag gagcccgccg gcgatctgca ggaattttgc gtaatccaga
     3181 atgagatggg cgatcgattg aatgccgtag gcggcaattc cggcaatcag cacgtcggcg
     3241 agcaccgcgc cgaggcctgc aagaaaggcg agcttcacgc cgtggcacag ggcggtgcgg
     3301 atgacaataa gattgaccgg gcccaggggc gccgccatgg caatgcctat ccctgtgcca
     3361 atgatgatca acgacaagtc catgatctag cggatatcca caactacacg gccgcgaacc
     3421 tggcccgcga ctatgtctgc ggccacgcgc ggcacatctt caagcttcac atgggtggtg
     3481 agagcggcaa gcgccttgag atcgagatcg cgggcaatgc ggttccaggc ctgctggcgg
     3541 cgcggtagcg gcgtggtgac ggagttgatg ccgatgagtt tcacgccgcg caggatgaag
     3601 ggggcaaccg aggtcggcag atccatgccc tgggccaggc cgcaagcggc gacggcgccg
     3661 tcattgttga tttgcgagat gacattggcc agcgtagtgg agcccacgga atcaatggct
     3721 ccagcccagc gcatcttggc cagcggcttt acgggcacgt tgaactcgtt gcggtcaatg
     3781 attgtggagg caccaagttt tttaaggaat tcggattctt ccggacggcc agtggaggca
     3841 gcaaccttgt agccaagttt cgccagaagc acgattgaaa cagtgccaac gccacctgca
     3901 gcgccggtca ccaggatttc accatcgccg ggctttaccc cctgctcttc cagcgccatg
     3961 acacacagca tggcggtgta gccggcaacg ccaacggcca ttgtatcggc acaggtccag
     4021 cccttcggta cggcgacgag ccagtcaccg gagacgcggg ccatttcgga aaagccaccg
     4081 ttgtgctgtt cgccgacacc cc
//
LOCUS       DAORVN010000089         4082 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_16970_length_4082_cov_3.411969, whole genome shotgun sequence.
ACCESSION   DAORVN010000089 DAORVN010000000
VERSION     DAORVN010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 4082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4082
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16970_length_4082_cov_3.411969"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            23..1261
                     /locus_tag="PKV23_02690"
     CDS             23..1261
                     /locus_tag="PKV23_02690"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007601216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA];
                     GO_process: GO:0019547 - arginine catabolic process to
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HQX84371.1"
                     /translation="MTTPAPLGAYSEIGKLRTVLTCRPGLAHQRLTPTNCHDLLFDDV
                     FWVHEAKNDHHDFVVKMEERGVEVLEMHKLLSETLTDKTALNWLLDRKFTENQVGIGM
                     VGELKSWLAGLPTTDLAEFLTGGITVSDIPFKTQGMFGLYLGPHGFVTPPLPNLMFTR
                     DTSAWIFGGVTLNPMHWPARRQETLLTAFVYKFHPRFKAKDVKVWWGDVERDFGPATL
                     EGGDIMPIGNGAVFVGMGERSTPQAVGQLARSLFAAGVATRVVACQMPQSRAAMHLDT
                     VFTLCDREVATSFVEAAEQLRCYSLRPGDRANSVDVKAEHKSVFELVAECLEIKKLHI
                     VSTGGDAAEQEREQWDDGNNVIALEPGVVIAYDRNVYTNTLLRKAGVEVITIRGAELG
                     RGRGGGHCMTCPVSRDGVDY"
     gene            1273..2274
                     /gene="argF"
                     /locus_tag="PKV23_02695"
     CDS             1273..2274
                     /gene="argF"
                     /locus_tag="PKV23_02695"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011983033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HQX84372.1"
                     /translation="MPFSLQDRSFLTLRDFTPAEIKFLLKLSADLKSAKYTGTEQQRL
                     KGKCIALIFEKASTRTRVGFEVAAYDQGAHVTYLGPSGTQIGHKETMKDTARVLGRIY
                     DAIEYRGFSQKTVEELAKFSGVPVYNGLTDQYHPTQVLADLLTMQEHSEKPLSQVSFC
                     FLGDAGNNMGDSLLIGGAKMGMDVRLCAPKSCWPDKEIIANAEKTAAETGARVMVTEN
                     VEQAVKGCDFLYTDVWLSMGEPEEKWAERIKLLKPYQVSMQVMKLTGKPSTKFMHCLP
                     AFHNTETEVARDIEKKFGLTAMEVTEEVFESPASIVFEQAENRMHTIKAVLVATIGT"
     gene            2279..3187
                     /gene="arcC"
                     /locus_tag="PKV23_02700"
     CDS             2279..3187
                     /gene="arcC"
                     /locus_tag="PKV23_02700"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018063324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HQX84373.1"
                     /translation="MRVVIALGGNAILKRGEVMSAENQRQNVRRAALAIAEVIAAGHK
                     VVVTHGNGPQVGLMALQDMAYDQALASPVDVLGAETEGMIGYLIEQELGNVISAPNKI
                     ATLLTQVEVDASDPAFRKPSKPIGPLYGSKEATALAEKHGWVMVPDGSHFRRAVPSPQ
                     PKRILEIGVIRLLIEHDVTVICTGGGGIPVVKRKDGSYAGVEAVIDKDHASGLLAREI
                     DADALLMLTDVDGVYLGWGTAQQRRLESVKPGELMSYAFAAGSMGPKARAAADFVSGG
                     GKMAGIGRLEDALGILNRSAGTHIVA"
     gene            complement(3196..>4082)
                     /locus_tag="PKV23_02705"
     CDS             complement(3196..>4082)
                     /locus_tag="PKV23_02705"
                     /inference="COORDINATES: protein motif:HMM:NF026066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HQX84374.1"
                     /translation="MKTNNQVVAKALREAAALFAQQDANKFRVSAYRNAAKTIEDLPE
                     DVADVAARGPGALDALPHIGKSIARAILQLVTTGRWPQLERMRGELDPEASFQNIPGI
                     GPAFARLIHEHLHIDTLESLEAAAHDGRLDTVPGIGERRGKIIRQSLASMLARKGPLP
                     SGLHKAPPPPIGMILEVDREYRERAKAGDLKFIAPRRFNPKGEAWLPILHTERGPWHF
                     TTLFSNTARAHELGKTADWVIVFYASDHQNEDQCTVVTETNGPLKGQRVVRGREPECL
                     AQLQQSGPDTGNLRHSGS"
BASE COUNT          831 a         1144 c         1294 g          813 t
ORIGIN      
        1 gggttccgga aaggaacagc taatgaccac tccagcaccg ctcggcgcat actctgaaat
       61 tggcaagctc cgaacggtgc tgacctgccg tcccggcctg gcccaccaga gattgacgcc
      121 gacaaactgc catgaccttt tgtttgacga cgtcttttgg gttcatgaag ccaagaatga
      181 tcatcatgat ttcgttgtca agatggaaga gcgtggggtt gaggttctgg agatgcacaa
      241 gctcctgagc gagaccctga cggacaagac ggcactcaac tggctgcttg accggaaatt
      301 cacagagaac caggtcggca ttggcatggt tggagaattg aaaagctggc ttgcaggact
      361 ccccaccact gatctggccg agttccttac cggcggaatc accgtgagcg acatcccctt
      421 caagactcag ggcatgtttg gcctctatct cggtccgcat ggatttgtca cgccgccgct
      481 gcccaatctg atgttcaccc gtgataccag cgcctggata ttcggtggcg taacactcaa
      541 ccccatgcac tggccggcgc ggcgccagga aaccctgctg actgccttcg tttacaagtt
      601 tcatccgcgc ttcaaggcga aagacgtaaa agtgtggtgg ggcgatgtgg agcgggattt
      661 tggcccggca acccttgagg gcggcgacat catgcccatt ggcaatggcg cggtgtttgt
      721 cgggatgggc gagcgcagca cgccgcaggc ggtaggccag cttgcgcgtt cattatttgc
      781 ggcaggcgtt gcaacgcggg ttgttgcctg ccagatgccg caatcacggg ctgccatgca
      841 tcttgatacg gtgttcaccc tgtgcgaccg cgaggtggca accagtttcg tcgaggccgc
      901 ggaacagctt cgctgctaca gcttgcgtcc gggcgacagg gccaattccg tcgatgtcaa
      961 ggccgagcac aagtcggttt ttgaactcgt ggccgaatgc cttgagatca agaaattgca
     1021 tattgtttca accggcggcg atgccgccga gcaggagcgc gagcagtggg atgacggcaa
     1081 caatgtcatc gccctcgaac ccggcgtggt gatcgcctat gaccgcaatg tctataccaa
     1141 taccttgctg cgcaaggccg gggttgaagt cattaccatc cgcggcgccg aactgggccg
     1201 cgggcggggc gggggacact gcatgacctg cccggtctcc cgcgatggcg tggactatta
     1261 ggagaaacac caatgccatt cagtctacaa gaccggagtt tcctgacgct tcgcgacttc
     1321 acaccggcgg aaattaaatt tcttctcaag ctctcggctg acctcaagtc cgccaaatac
     1381 accggcacgg agcagcagcg gctcaagggg aaatgcatcg cgctgatctt cgagaaggct
     1441 tccacccgca cacgggtcgg attcgaggtc gccgcctatg accagggcgc ccatgtgact
     1501 tatctgggtc cgagcggcac gcagatcggc cacaaggaaa cgatgaagga cacggcccgg
     1561 gtgcttggcc gaatctatga cgcgatcgag tatcgcggtt tcagccagaa gaccgtggag
     1621 gagcttgcga aattctccgg cgtgccggtc tacaacggcc ttacggacca gtaccatcca
     1681 acgcaagtcc ttgccgatct gctcaccatg caggagcaca gcgagaagcc gttgagccag
     1741 gtgagtttct gctttctcgg cgatgccggg aacaatatgg gggattcact cctgatcggc
     1801 ggggccaaga tgggaatgga cgtgcgcctc tgcgcgccaa aatcctgctg gccggacaag
     1861 gaaatcattg ccaacgccga gaagactgcg gcggagaccg gggcccgggt gatggtgacg
     1921 gaaaatgtcg aacaggcggt gaaaggctgc gacttccttt acaccgacgt gtggctttcg
     1981 atgggcgagc cagaggagaa gtgggcagag cgcatcaagc tgctgaagcc gtaccaggtg
     2041 agcatgcagg tcatgaagtt gacggggaaa ccctccacca agttcatgca ttgcctgcct
     2101 gcattccaca atacggagac ggaagttgcc agggacatcg agaagaaatt cggactgacg
     2161 gccatggaag tcaccgaaga agtcttcgaa tcgccggctt ccatcgtctt cgagcaagcc
     2221 gaaaaccgga tgcatacgat caaggcggtc cttgtggcga cgattgggac ctgacggcgt
     2281 gcgcgtggtc atcgcacttg gcggaaacgc aattctcaag cgcggcgagg tgatgtcggc
     2341 ggagaaccag cgccagaacg tcaggcgtgc ggccctggcc atcgcggagg tgattgcggc
     2401 cgggcataaa gtcgtcgtga cgcatggcaa cggcccacag gttggcctca tggcgcttca
     2461 ggatatggcc tatgaccagg cccttgccag tccggtcgat gttctgggcg ccgaaacgga
     2521 aggcatgatc ggctatctga ttgaacagga actgggaaat gtcatatcag cgcccaataa
     2581 aattgccacc ctgctgacgc aggttgaagt cgatgcctcg gaccctgcct tccggaagcc
     2641 aagcaagccg atcggccccc tgtatggcag caaggaggca acggcattgg ccgagaagca
     2701 tggctgggtc atggtgcccg atggatccca tttcaggcgg gccgtgcctt cgccgcagcc
     2761 gaaacgcatt ctggagatcg gcgttatcag gctgctcatc gagcacgatg tgaccgttat
     2821 ctgcacgggg ggcggcggca ttccggtcgt gaagcgaaag gacggcagtt acgcgggggt
     2881 tgaagccgtt atcgacaagg accacgcaag cggcctgctg gcgcgtgaaa tcgatgcgga
     2941 cgccctgttg atgctgaccg atgtggatgg cgtctacctt ggctggggaa cagcgcagca
     3001 gcggcgcttg gagtccgtaa agccgggcga gctgatgtcc tatgccttcg cggccggaag
     3061 catggggcca aaggccaggg ctgcggccga ctttgtgtca ggcggcggaa agatggcggg
     3121 tatcggccgg cttgaagatg cgctcgggat attaaaccgg agcgctggca cccacattgt
     3181 ggcataggcg tgcggctatg atcctgagtg ccgcagattt ccagtgtcag gcccggattg
     3241 ttgcagctgt gccaggcatt ccggttcgcg gccccgtaca acccgctggc ccttgagcgg
     3301 gccatttgtt tcggtgacaa ccgtgcattg gtcttcgttt tgatggtcgg acgcgtagaa
     3361 gacaatgacc cagtcggccg tctttcccaa ttcatgggct cttgcggtgt ttgaaaacag
     3421 cgtggtgaaa tgccaggggc cgcgctccgt atgcaggatc ggaagccatg cctcgccttt
     3481 cgggttgaaa cgccggggcg ctatgaattt gaggtcaccg gctttcgccc gttcccggta
     3541 ttccctgtca acctccagga tcatcccgat tggcggcggc ggcgccttgt gaaggccgga
     3601 gggcagcgga cctttgcgcg ccagcatgct ggccaatgac tggcggatga tcttgccccg
     3661 gcgctcgccg atccccggaa cggtgtccag tctcccatcg tgcgccgcgg cctcgagcga
     3721 ttccagcgtg tcgatgtgca ggtgttcatg aatgagccgg gcaaaggccg ggccaattcc
     3781 aggaatgttc tgaaatgacg cttcaggatc gagttctccg cgcatccgtt caagctgcgg
     3841 ccatctgccg gttgtcacga gctgcaggat ggcacgggct atggattttc cgatgtgggg
     3901 cagggcgtcg agagcacccg gcccccgtgc ggcgacatct gccacatctt ccggcaggtc
     3961 ctcaatggtt tttgcggcat tgcgataggc cgagacgcgg aatttgttgg catcctgctg
     4021 ggcgaaaagt gccgcggctt cacgcagggc tttggcgacc acttgattgt ttgtcttcat
     4081 tc
//
LOCUS       DAORVN010000090         4076 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_17014_length_4076_cov_3.294454, whole genome shotgun sequence.
ACCESSION   DAORVN010000090 DAORVN010000000
VERSION     DAORVN010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 4076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4076
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17014_length_4076_cov_3.294454"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1207
                     /locus_tag="PKV23_02710"
     CDS             <1..1207
                     /locus_tag="PKV23_02710"
                     /inference="COORDINATES: protein motif:HMM:NF021603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2336 domain-containing protein"
                     /protein_id="HQX84375.1"
                     /translation="AEIAALLAADAARSKIQADSEVVIVEPASAQKSRVRRVERHDDP
                     FAKLEIESPAPPEIIDESDADAGELARSLLDMMLSNPSAGQPQERALAADALLKLVPR
                     IPLKSLVMVVDRISIMESPPHLLVSKLIHDPRIEVAGPLLELCNHINDQVLSKVIATG
                     QVSLLRLIARRRHISANLSDQLIEFDDPSVLLTLVRNSGAVFSHHAFPRLADHAQRHQ
                     ALLAPLATRPDLPAPIAFELFWFVPAELRRYLLSRFLTDSETLTKILKITHAMGGGEQ
                     GTETRFPDREQIEAFVEFLNAGKFPEASNLLAEITAIQPDSASRIIADSNGEPLTIAL
                     KAVGTNRFRFGEIIDMLKASDFGIISSDRSTAELQNIFDSMSFNKARVLLTYWDWAVM
                     KSGPYAPAN"
     gene            complement(1220..1846)
                     /locus_tag="PKV23_02715"
     CDS             complement(1220..1846)
                     /locus_tag="PKV23_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016844081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HQX84376.1"
                     /translation="MILIGQYDSPFVRRVGIALTLYELPFEHRPWSTFGEADKLRPYN
                     PLTRVPTLVLDNGESLIESHAILDYLDSLVPPEKALFPRDEPARHQALKIAALATGLG
                     DKAVSLFYERLLHTETSEAWSNRCRQQIAAVLTVLEEDRASRTTDYWFGGRMGHADIA
                     VATVLGFVVEAHPGLVGLSDFPGLNAHAVRLEALPVFQAIKQPFIPPN"
     gene            complement(1895..3079)
                     /locus_tag="PKV23_02720"
     CDS             complement(1895..3079)
                     /locus_tag="PKV23_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009737067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HQX84377.1"
                     /translation="MPYNFDEYVLDTGRRELRRGAELIDAEPQVFDLLLYLIENQDRV
                     VSGEELFDAVWQGRIVSLSTLTSRINAARRAIGDSGAKQKLIKTIPRKGYRFAGEAKL
                     EAGQFAAAAQPEAALRQEVQFCICGDGVRIAYATVGSGPPLVKAGNWLNHLEYDWDSP
                     VWRHLLTWMASERQFIRYDARGNGLSDWDADDISFEAFVRDLETVVDAAGLERFALFG
                     ASQGCAVSIDYAARHPERVSKLVLYGGFARGKRLRDDPTVIAQSDALITLMRTGWGQE
                     NPAFRQVFTSLFVPGGTAEQMRWFNDLQRNTTSPENAVRIRLVSDWIDVRNLLPQITM
                     PTLVLHCRDDAVQPFEEGRMMAAGIPGARFVCLEGRNHVILESDPGWSRFRQEIDSFL
                     AD"
     gene            3237..>4076
                     /locus_tag="PKV23_02725"
     CDS             3237..>4076
                     /locus_tag="PKV23_02725"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HQX84378.1"
                     /translation="MFHNSPTDARARPLGRNFMVRALLPWDMFFNAMTRRKTRFLLSA
                     LDDHLLKDIGISRGEIDRIARDTAGEPGIARTARQPTPTITQLNQRRTTMTTKTATSQ
                     KPARTPVAMNGVDTPTLLATINAVGGQPELAKFQFRAKSRWISGTHSQSTMEGFFGAG
                     GEHKHKAAYKADGDHPAVLCGVDNGPTPVEWVLHALASCLTSGIANIASARGIKLRKV
                     ESTVEGDIDLRGILGLSDKVRNGYQGIKVSFDIDGDASPEQLEKIIMQSKARSAVFDV
                     ITNG"
BASE COUNT          807 a         1367 c         1152 g          750 t
ORIGIN      
        1 ggccgagatt gccgccctgc tggccgccga tgcggcacgc agcaaaatcc aggccgattc
       61 cgaagtggtt atcgtcgaac cggcttccgc gcaaaaatcc cgggtgcgcc gcgtcgagcg
      121 gcacgatgat ccatttgcaa agcttgagat cgaaagccct gcaccgcccg aaatcattga
      181 tgagtccgat gcggatgcag gcgaactcgc ccgctcgctt ctcgacatga tgctgtcaaa
      241 ccccagcgcc ggccagccgc aggagcgggc ccttgccgcc gatgcccttt tgaaactggt
      301 gccgcgcatc ccgttgaagt ccctggtcat ggtcgttgac cgcatttcca tcatggaatc
      361 gccgccgcat ctgcttgtct ccaagctgat ccatgacccg cgcattgaag tggccggccc
      421 cttgctcgaa ctctgcaacc acatcaacga ccaggtcctg tccaaggtca ttgccacggg
      481 gcaggtgagc ctcctgcgcc tgatcgcgcg ccgccgccat atttcggcca acctgagcga
      541 ccagctcatc gaattcgacg acccgtcggt ccttctcacc ctggtgagaa acagcggcgc
      601 cgtcttctcg caccatgcat tcccccgtct tgccgatcat gcccagcgcc atcaggcgct
      661 cctcgcgccg ctggctaccc gccccgatct gccggcccct atcgcctttg aacttttctg
      721 gttcgtgccg gcggaactcc gccgatatct cctgtcgcgt ttcctgaccg actcggaaac
      781 gctcaccaag atcctgaaaa tcacccacgc catgggcggc ggcgagcagg gcacggaaac
      841 ccggttccct gaccgcgaac agatcgaggc tttcgttgag ttcctcaatg caggcaagtt
      901 ccccgaggcc tcaaatctgc tggccgaaat caccgccatt cagcccgaca gcgcctcgcg
      961 catcatcgcc gattccaacg gcgagccgct gaccatcgcc ttgaaggcag ttggcaccaa
     1021 ccgtttccgc ttcggcgaga tcatcgacat gctcaaggcc tccgacttcg gcatcatttc
     1081 ctcagaccgg agcaccgccg aactgcagaa catttttgat tccatgtcgt tcaacaaggc
     1141 ccgggttctg ctcacctatt gggactgggc cgtaatgaaa tccggcccct acgccccggc
     1201 caattgaggc agagacaact caattcggcg gaatgaatgg ctgtttgatc gcctggaaca
     1261 ccggcagggc ctcgagccgc accgcatggg cgttgagccc cggaaaatct gaaagcccca
     1321 ccaatcccgg atgcgcttcc acaacgaaac ccagcactgt ggccaccgcg atatcggcat
     1381 gccccatgcg gccgccaaac cagtagtcgg tcgtccggct ggcgcggtct tcttccagca
     1441 cggtgagcac ggcggcaatt tgctggcggc aacggttgct ccaggcttct gacgtttccg
     1501 tgtgcagcag cctttcgtag aaaaggctga ccgccttgtc gccaaggccc gttgccaggg
     1561 cagcgatctt cagcgcctgg tgccgggcgg gttcgtcccg tggaaacagg gccttctccg
     1621 gcggcacgag actgtcgaga taatccagaa tggcatggct ttcgatcaga gactcgccgt
     1681 tatccagcac cagcgtcggg acccgggtga ggggattata gggccgtagc ttgtccgcct
     1741 cgccgaatgt ggaccagggc cggtgctcaa atggcagctc atacagcgtc agcgcgatgc
     1801 cgacacggcg aacaaagggc gagtcatact ggccgatcag gatcatctcg ggacgttgcc
     1861 agcgatgggc ccgagcggca agtgccgtgg cgctttaatc cgccagaaaa ctgtcgatct
     1921 cttgcctgaa gcggctccag ccgggatccg attccaggat cacatggttg cgcccctcaa
     1981 ggcacacgaa gcgcgccccc ggaatgcccg ccgccatcat ccggccttct tcaaacggct
     2041 gcaccgcatc gtcacggcaa tgcagcacca gggtgggcat ggtgatctgc ggaagaagat
     2101 ttctgacatc gatccagtcc gacaccagcc ggatgcgcac ggcattttcg ggtgatgtcg
     2161 tattgcgttg caggtcattg aaccagcgca tctgttcggc cgttccgccg ggcacgaaga
     2221 gcgaggtgaa gacctggcgg aaggcgggat tttcctggcc ccagcccgtg cgcatgaggg
     2281 tgatcagggc atcggactgg gcgatgacgg tgggatcatc ccgcagccgc ttgccgcggg
     2341 cgaacccgcc gtagagcacc agcttgctca cccgctccgg atgcctggcg gcatagtcaa
     2401 tgctgaccgc gcagccctgc gaggcgccaa aaagggcaaa gcgctcaagc cccgccgcat
     2461 cgaccaccgt ttcaagatcg cgcacgaagg cctcgaagga aatgtcatcg gcgtcccagt
     2521 ccgacaggcc gttgccgcgc gcgtcgtagc ggatgaactg gcgttcgctg gccatccatg
     2581 tcagcagatg cctccacacc gggctgtccc agtcatattc cagatggttc agccagttgc
     2641 cggctttcac cagcggcggc ccgctgccga ccgtggcata ggcaatgcgc accccgtctc
     2701 cgcaaatgca gaattgtacc tcctggcgca aagcggcttc cggctgcgcg gcagccgcga
     2761 actgcccagc ttcgagtttg gcctcgccgg caaagcggta tcccttgcgc ggaatggtct
     2821 tgatcagttt ctgttttgcg ccgctgtcgc caatggccct gcgcgccgcg ttgatgcggc
     2881 tggtgagcgt tgaaagcgag acaatacgcc cctgccatac ggcatcaaac agctcttcgc
     2941 cgctgaccac cctgtcctga ttctcgatga gatagagcag caggtcaaaa acttgcggtt
     3001 cggcgtctat gagctcggct ccccgccgca attcgcggcg cccggtatcc agcacatatt
     3061 catcgaaatt ataaggcacg cagatctcct ctgccccccg ccgcgccttc tagcgcgtta
     3121 atcaagggct tctcaaggaa aaaacaagcc cgcctcaaag cccccgggag cggtggccgt
     3181 ctagatgtcg ctccatgaac cgggccctct aggtccagca aaaaaagggc catgccatgt
     3241 ttcataattc gccgactgat gcccgcgcgc ggcccctggg ccggaatttc atggtccgtg
     3301 cgctgctgcc ttgggacatg tttttcaacg cgatgactcg ccggaaaacc aggttcctgc
     3361 tgtctgcgct ggacgatcat ctgctcaagg atatcggcat ttcgcggggc gagatcgacc
     3421 ggattgcccg cgatactgct ggcgagcccg gcatcgcccg cacagcgagg caacccacac
     3481 ccactattac ccaactcaac cagaggagaa cgaccatgac gaccaagacc gcaacttccc
     3541 agaaaccagc ccgcacgccc gtagccatga atggcgttga cacaccaacc ctcctggcca
     3601 ccatcaatgc cgtgggcggc cagcccgagc ttgccaagtt ccagttccgc gccaaaagcc
     3661 gctggatttc cggcacccac agccagagca ccatggaggg tttcttcggc gccggcggcg
     3721 aacacaagca caaggctgcc tacaaggccg atggcgatca tcccgccgtt ctctgcggtg
     3781 tcgataacgg gcccacgccc gttgaatggg tcctgcacgc gctggcttcc tgcctgacct
     3841 ccggcatcgc caacatcgcc tcggcccgcg gcatcaagct gaggaaagtc gaatcgaccg
     3901 tcgaaggcga catcgatctg cgcggcatcc tgggtctgtc ggacaaagtc cgcaacggct
     3961 accagggcat caaggtctcc tttgacatcg atggcgatgc cagccccgag cagctggaaa
     4021 agatcatcat gcagtcaaag gcgcgctcgg ccgtcttcga cgtcatcaca aacggc
//
LOCUS       DAORVN010000091         4076 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_17015_length_4076_cov_3.220592, whole genome shotgun sequence.
ACCESSION   DAORVN010000091 DAORVN010000000
VERSION     DAORVN010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 4076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4076
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17015_length_4076_cov_3.220592"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1122)
                     /locus_tag="PKV23_02730"
     CDS             complement(<1..1122)
                     /locus_tag="PKV23_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008839133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HQX84379.1"
                     /translation="MLAPEVETRPWAEQFVIDDAQYRNQLDYLLAHSAFYQKKFEAAG
                     IGPSGAGGLADIGQLPFTEKSEIRASCTAENPIGSHLCVGRSEIMRIYSTSGTTGTPS
                     YIPLTARDLENWVTTSARSYSASGVEAGQRIVSTYNAGPFVAGAALAAFDKLAMCHIP
                     VGTGNTERLMTAIRLLKPEAVVLTPSYAAYLIEWAAEKDFDLKGSSVRRVLVAGEPGG
                     GEPAFRKKLEEGWGARVTEAMGIGDIGVSLWGECEYQQGMHLGGRGFIHAELIDPDSG
                     ASLTMRDGAKGELVLTHLQHRAAPLLRFRTRDHVEVWTSACKCGRTAPRIRCIGRTDD
                     MLIVRGVNVFPSAIREVVSEFVPDVSGHILVKPSQPGVKQ"
     gene            complement(1122..1919)
                     /locus_tag="PKV23_02735"
     CDS             complement(1122..1919)
                     /locus_tag="PKV23_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015888159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citryl-CoA lyase"
                     /protein_id="HQX84380.1"
                     /translation="MQIGKTGTAESHICKAYPDRVEVRGLDLCRDLMGQMSFTEYFHL
                     LLTGRKPTELQRDFLDLLLVAISEHGMMPTSVAARMTLAADPGSLQGALAAGLLGCGP
                     VILGTAELCAKLLAEAQAKVLSGGNVKTVAEQIALDIKSAGGRAPGFGHPVHRPLDPR
                     TELIFELADQRGVSGPHVALARQFRLAIAKVWGKPLVMNVSMPMAAVLLDMGLPVNIV
                     KAIPLLARTAGILGHLAEEQENPVGLLMAGKAEEAITYRSDQPEDKA"
     gene            complement(1989..3518)
                     /locus_tag="PKV23_02740"
     CDS             complement(1989..3518)
                     /locus_tag="PKV23_02740"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HQX84381.1"
                     /translation="MLSPPALAGPYEDAAAAYARRSYTEALLLWQELAAQGNAAAQYN
                     LGALYENGQGVPKDSAAAERWYRLAAGQGLADAQFTLGLRYANGQNMSEALKWYRLAA
                     EQGFTQAQYNLGAIFDEANGVRRDFSEAAKWYRLAADNGSAAAQANLGLMHEVGRGVR
                     LDFAEAANWYQRAAEQGHALAQSRLGLLYDQGRGVEQDFAKALKWYRLAADQGNAAAQ
                     NNLGVLHAQGKGVEKNDSEAVKWYGLSAEQGFAAAQANLGAAYDMGLGVAQDATEAIK
                     WLGMAADSAYAPAQHSLGAMAANGRGGPPDYAAAARWYRLAAVQDFAPAQFDLGASYE
                     NGVGLPQDHGQAVKWYRLAAAQGHVAAQFRLGVSYDLGQGVTQDYVEAIKWYRAAVAQ
                     GDAAAQNNLGSMYEYGLGVAQDYVEAVKWYQFSADQGYAKAQYNLGLMHANGRGVPPD
                     FMIAHQWLDLAIEADPPLAPKDMDDATDNRGQIAARLAPEELAQAQRRAREWLAAHPK
                     H"
     gene            complement(3674..>4076)
                     /locus_tag="PKV23_02745"
     CDS             complement(3674..>4076)
                     /locus_tag="PKV23_02745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX84382.1"
                     /translation="SGAMEYYADRLLRSDAVGGTTAVSPDAQYNAQVGRILARNVAGP
                     TVDVTDRSYLAREISARTGLAETDAQKRVDDTVATLKAQADTARRYGVLLAFMTAASL
                     LISGVAAWWAATAGGKHRDDGVDHSHMSRWS"
BASE COUNT          795 a         1248 c         1234 g          799 t
ORIGIN      
        1 ttgcttaacg ccaggttgtg atggctttac cagaatatgg ccgctgacat cgggcacaaa
       61 ttcactgacc acctcacgga tggccgaggg aaaaacattc acgccgcgca cgatgagcat
      121 atcgtcggtg cggccgatgc aacggatgcg aggggcggtg cggccgcatt tgcaggcact
      181 ggtccagact tccacgtggt ccctggtgcg gaaacgaagc aagggtgcgg cccggtgctg
      241 gagatgggtg aggacaagtt cgcccttggc gccatcacgc attgttaggg atgcgccact
      301 gtcagggtcg atcagttccg catggatgaa accgcgacca ccgagatgca tgccctgctg
      361 atattcgcat tcgccccaga gggagactcc gatgtcgccg atgcccatgg cttcggtaac
      421 ccgggctccc catccctctt ccagcttctt gcggaaggca ggttcgccgc caccgggctc
      481 acccgcaacc agtacacgcc gcacacttga tcccttcagg tcaaaatcct tctccgctgc
      541 ccattcaata agataggccg cataggaggg ggtgagtaca acggcttcgg gcttcagcag
      601 gcggatggct gtcatcaggc gctcagtgtt gccggtgcca acgggaatat ggcacatggc
      661 caatttatcg aatgcggcca gcgcagcacc ggccacgaat ggcccggcat tgtaggtgga
      721 aactatgcgc tggccggctt cgacgccgga ggccgaatag cttcgtgccg aggtggtcac
      781 ccagttctca agatcgcgcg cggtcaatgg aatgtagctt ggcgttccgg tggtgccgct
      841 ggtcgaatag atgcgcatga tttccgaacg gcccacgcag aggtgggaac cgatggggtt
      901 ttctgctgtg cagcttgccc tgatttcgct tttttcggta aagggaagtt gaccaatgtc
      961 agcaagtccc cctgctccag acgggccaat gcctgccgct tcgaacttct tctgataaaa
     1021 agcagagtgc gccaggagat agtcgagctg attgcggtac tgcgcatcgt cgatgacaaa
     1081 ttgctccgcc catggacggg tttcaacttc cggcgccagc atcacgcctt gtcttccggc
     1141 tgatcgctgc gataggtgat cgcctcctcc gccttcccgg ccatgagtaa gccaaccgga
     1201 ttttcctgtt cttcggcgag atggccaaga atgccagcag tgcgcgccag caacgggatg
     1261 gctttcacga tgttcacggg taagcccata tccagcagca cggcggccat tggcatggag
     1321 acattcatga ccagcggctt gccccagacc ttggctatgg caaggcggaa ctgccgcgcc
     1381 agggccacat gggggccgct gacaccacgc tgatcagcga gttcgaaaat gagctcggtg
     1441 cgcgggtcaa gcggacggtg cacgggatgg ccaaagcccg gggccctgcc gcccgcgctc
     1501 ttaatgtcga gggctatctg ctctgcaacc gtcttaacgt taccgccgga aagcaccttg
     1561 gcctgcgctt cggccagcag cttggcacaa agttcagcgg tgcccaggat caccgggccg
     1621 caacccagca gtccggcagc aagggcgccc tgcaacgagc cgggatcggc ggcgagcgtc
     1681 atgcgcgcgg caactgaggt gggcatcatg ccgtgctcgg agatggcaac gagcaagagg
     1741 tcaagaaaat cccgctgcaa ttcggttggt ttgcgcccgg tgagaagcaa atgaaaatat
     1801 tcggtgaagc tcatctgccc cataagatcg cggcacaggt ccagcccgcg cacttcgacg
     1861 cggtccggat aggccttgca gatatggctc tctgctgtgc cggtcttgcc tatctgcatt
     1921 tatcaccctg ggctgaattc aaaccgccac atcatatatt gaccccggct ttcggcaagg
     1981 cccgccggtc aatgcttggg gtgcgccgcc agccattccc gcgcgcggcg ttgcgcctgg
     2041 gccagttctt cgggtgccag gcgcgcggct atctggccgc gattgtcggt cgcatcgtcc
     2101 atgtccttcg gtgccagcgg aggatcggcc tctatcgcaa gatccagcca ctggtgggcg
     2161 atcatgaagt ccggcggcac gccgcggccg ttggcatgca tgaggccaag attatactgg
     2221 gccttggcgt agccctgatc agccgagaat tggtaccatt ttacggcttc aacgtagtcc
     2281 tgggcaacac cgaggccata ctcatacatg gaaccaagat tgttctgggc tgcggcatca
     2341 ccttgggcga ccgccgcgcg gtaccacttt atcgcttcaa cataatcctg ggtaacaccc
     2401 tggccgaggt catagctgac tcccagtcta aactgggcag caacatggcc ctgcgcggcg
     2461 gcaaggcggt accacttgac ggcctgacca tggtcctggg gcaggccaac gccgttttcg
     2521 tagctggcgc ccaaatcaaa ctgggcgggg gcaaagtcct ggacagcagc aaggcggtac
     2581 cacctggctg ccgcggcata atccgggggg ccgccacgcc catttgcggc cattgcgcca
     2641 aggctgtgct gggcaggtgc ataagcgctg tcagcggcca tgccgagcca cttgattgcc
     2701 tctgtggcgt cctgggcaac gcccaggccc atgtcatagg cggcaccgag gtttgcctgg
     2761 gcggcggcga agccctgttc ggctgacagc ccataccatt ttaccgcctc agaatcattc
     2821 ttctccacgc ccttaccctg agcgtgcaaa acgccaaggt tgttctgggc ggcggcgttg
     2881 ccctgatcag cagcgagcct gtaccatttc agggccttgg cgaaatcctg ctccacgcca
     2941 cggccctggt catataataa ccccaagcgg ctttgcgcga gggcgtggcc ctgctcggcg
     3001 gcgcgctgat accaattggc ggcttcggca aagtccagcc gcacaccccg gccgacctcg
     3061 tgcatcaggc caagattggc ctgggcagcc gccgatccat tgtcggccgc caaccggtac
     3121 catttggcgg cttcagaaaa gtctcgccga acaccattgg cttcatcaaa gatggcgcca
     3181 agattgtact gggcttgggt aaacccctgt tctgcggcca gacgatacca tttcagcgct
     3241 tccgacatgt tctggccatt ggcgtaacgg agtccaaggg tgaattgggc atcggcaagg
     3301 ccctggccgg cagcaagccg gtaccatctt tcggctgcgg cgctgtcttt tggaacaccc
     3361 tggccgttct cgtacagggc cccaagattg tattgggcgg cggcgttgcc ctgcgcggcc
     3421 aattcctgcc agagcagaag cgcctcagtg taactccggc gggcgtaagc ggcggcggca
     3481 tcttcgtagg gcccggccag agcgggcggt gacaacataa tgatcgtcag ggcaagaatc
     3541 ttaaagggta aaccagatat catgcggcga gcattaacaa catttcccag gcggtgtcac
     3601 gttaagccgg tcgacctcgg gtaagacttt gggtgtaatc ccaccacctt tcggcagcgg
     3661 ggcaccgtgg atgctaactc cagcgggaca tatggctatg gtccacgcca tcgtcgcgat
     3721 gtttgccacc cgcagttgcc gcccaccacg cagccactcc actgatcaat agagaggcgg
     3781 cagtcatgaa ggccagcaac acgccgtagc ggcgggcagt gtcagcctgg gcctttaaag
     3841 ttgccactgt gtcgtccacg cgtttttgcg catcggtttc cgccagtccc gtgcgggctg
     3901 aaatttcgcg cgccaaatag gacctgtcgg ttacgtccac ggtcggccca gcgacatttc
     3961 gcgcaaggat gcggccaacc tgggcgttgt actgggcatc gggcgaaacc gcggtagtgc
     4021 cgccaacggc atcgctccgc agcagccggt cagcataata ttccatcgca ccagag
//
LOCUS       DAORVN010000092         4025 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_17405_length_4025_cov_2.897481, whole genome shotgun sequence.
ACCESSION   DAORVN010000092 DAORVN010000000
VERSION     DAORVN010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 4025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4025
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17405_length_4025_cov_2.897481"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(131..1030)
                     /gene="speB"
                     /locus_tag="PKV23_02750"
     CDS             complement(131..1030)
                     /gene="speB"
                     /locus_tag="PKV23_02750"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007536252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HQX84383.1"
                     /translation="MRLPHVTLDEAKDLDIGLVGIPWDGGTTNRPGPRHAPRQMRDQS
                     SMVRRMHQTTHIVPYDLANIADLGDCSVNPASVDDALARVEGYFKKLVGKGIRPLSAG
                     GDHLCSLPILRAVGEKKPVGMIHFDAHTDLYDSYFGGFKYTHGTPFRRAIEEGVLDPK
                     RTIQIGLRGSMYDLDDFKYGEKMGVRLMRIEEAVELGPQKVMEIARRVVGDQETYVSF
                     DIDMLDPVYAPGTGTPEVGGFTTLQAQQMLRGLRGLNLVGADVVEVSPPFDASGMTAY
                     AGVVMMFEILCSMAETVAKQRRQ"
     gene            complement(1114..2295)
                     /gene="doeA"
                     /locus_tag="PKV23_02755"
     CDS             complement(1114..2295)
                     /gene="doeA"
                     /locus_tag="PKV23_02755"
                     /EC_number="3.5.4.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010975302.1"
                     /note="DoeA (degradation of ectoine A) is also called EutD
                     (ectoine utilization D); Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0042400 - ectoine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ectoine hydrolase DoeA"
                     /protein_id="HQX84384.1"
                     /translation="MTEATLHFPIAEYQRRIAKTRKAMQARGLDLILVSDPSNMEWLT
                     GYNGWSFYVHQAVLLGLEGEPVWWGRGMDANGARRTAFMGHDNIAGYPDHYVQSTERH
                     PMEHLSRLIADRGWKDALIGLEMDNYWFSAKAHTTLLKAFKPNNLQDATGLVNWQRSV
                     KSDLEIGFIRRAAKIVDAMHARVFELVEPGLPNNVLVAEIQKQSILGADGNWGDYAAF
                     VPMAPSGMDATAPHLTWDDRPFKKGEGTFFELGGCYRRYHAPLSRTVYLGKPPQKFLD
                     AEKAVLDATSAGLELAKPGKTCGDVADAFFDELKRHGFAKDSRAGYSIGLSYPPDWGE
                     RTMSIRRGDSTELEPNMTLHFMPALWLDDGGLELSETILITPQGAECLCQTPRKLVIK
                     N"
     gene            2358..3470
                     /locus_tag="PKV23_02760"
     CDS             2358..3470
                     /locus_tag="PKV23_02760"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HQX84385.1"
                     /translation="MADTFDIIVIGAGIAGASIAAHLAETKRVLILEMEDRPGFHTTG
                     RSAATFEPNYGPPPMLAFTRASNMFFTNPPQGFTDGPLLVPRSSLFFEAEGQENFTKN
                     LLASGSGLEELSETQARKLFPTLREGYAKRTFLDPHTGDLDVDLLHRGYLKLFKARGG
                     QLLNSAAAETILRQGHVWEVISPQGIFEADILVNAAGAWGDAVAAKAGVKPIGLQPKR
                     RSIGVIPVEGIPGVASFPMVTDVGETWYAKPQSGKMMVSSADATPVDPHDAYADDMAI
                     AEGIERLMNATTIEVNNLHHSWGGLRTFAPDGAPVIGFDPSTEGFFWLVGQGGYGIQS
                     APALSRTAAAMVLGEEPPQDVRDAGLVLAEILPDRF"
     gene            complement(3467..>4025)
                     /locus_tag="PKV23_02765"
     CDS             complement(3467..>4025)
                     /locus_tag="PKV23_02765"
                     /inference="COORDINATES: protein motif:HMM:NF017532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HQX84386.1"
                     /translation="AEIGRQLMGTKTARLFHEHVLVKEASAGVATPWHQDQPYYCVDG
                     NDTVSLWIPLDPVPRDRTLEFVGGSHKWGKYFRPERFDKTPLNENDGLEEVPDINGNR
                     DSFNVIGWALEPGDAVAFNYKTLHGAPANNSASEQRRAFSLRLLGDDARFARREGVKT
                     SPPFRGVTLAHGAVMDGPEFPVLVS"
BASE COUNT          787 a         1328 c         1130 g          780 t
ORIGIN      
        1 gtgcggatca gtgtcgccat gtcggaatta atctcggaca cgccgatctt ggcgaagatg
       61 gcggtaagcg ctgcaaacac cgccgacagg aacgcccagc tctgccagct catcaatccg
      121 gaattgatca tcactgcctc cgttgtttcg ccaccgtctc tgccattgag cacagaattt
      181 cgaacatcat cacaaccccc gcataggccg tcattcccga cgcatcgaag ggcggcgaca
      241 cttcaaccac atcggctccc accagattga gcccgcgaag cccgcgcagc atctgctgcg
      301 cctgcagggt agtgaacccg ccaacctccg gcgtgccggt gcctggcgca taaaccggat
      361 cgagcatgtc gatgtcgaag ctcacatagg tttcctggtc gcccacaaca cggcgcgcaa
      421 tttccatcac cttctgcggt ccgagttcca ccgcttcctc gatgcgcatg aggcgaaccc
      481 ccatcttctc gccatacttg aaatcatcga gatcatacat ggagccgcga agcccgatct
      541 ggatggtgcg ctttgggtca agcacgcctt cctcgatcgc ccgccggaac ggcgtgccat
      601 gagtatattt gaagccgccg aaatagctgt cgtaaagatc cgtatgggca tcgaaatgga
      661 tcatgcccac cggcttcttt tcgccgaccg cccgcaggat cggcagcgag cacagatgat
      721 cgccgcccgc cgaaagcggg cgaatgccct tgcccaccag ctttttgaaa tagccctcga
      781 cccgcgccag cgcgtcatcc acgcttgccg ggttgacaga acaatcgccg agatcagcga
      841 tattggcgag atcataggga acgatatggg tggtctggtg catccgccgc accatggagg
      901 attggtcgcg catctgccgc ggcgcatggc gcgggccagg ccggttggtc gtgccgccat
      961 cccacggaat gccaaccagc ccgatgtcga ggtccttcgc ctcgtccagt gtcacgtgcg
     1021 gcagccgcat gaaggtggcg atgccgccaa agcgtggcac cacaaggccg gaggcaggtt
     1081 gattgaattt tttcaaggca cgggttcttt cattcagttc ttgatgacaa gtttgcgcgg
     1141 cgtctggcac aggcactcag ccccttgggg cgtgatcagg atggtttccg acagttcaag
     1201 cccgccgtca tcaagccaca gggccggcat gaaatgcagg gtcatattgg gttccagttc
     1261 ggttgaatcg ccgcgccgga tcgacatggt gcgctcgccc caatccggcg gataggataa
     1321 cccgatggaa tagccggcgc gcgaatcctt cgcaaagcca tggcgcttga gttcatcaaa
     1381 gaatgcatct gccacgtcgc cgcaggtttt ccccggcttg gccaactcaa gcccggcgga
     1441 cgtggcatcg agcaccgcct tttccgcgtc gaggaatttc tgcggcggct tgcccaggta
     1501 aaccgtgcgc gacagcggcg catggtagcg ccggtagcag ccgcccagct caaagaaagt
     1561 gccctcgcct tttttgaacg gccggtcgtc ccatgtaaga tgcggcgccg ttgcgtccat
     1621 ccccgatggc gccatgggaa caaaggctgc gtaatcgccc caattgccgt cagcgccgag
     1681 aatggactgc ttttgaattt ccgcaaccag cacgttgtta ggaaggcccg gctccaccag
     1741 ctcaaaaacc ctggcatgca tcgcgtcaac aatttttgcc gcccgccgga tgaaaccaat
     1801 ttccagatcg ctcttcacgc tgcgctgcca gttcaccagg ccggttgcat cctgaagatt
     1861 gtttggcttg aaagccttca gcagtgtcgt atgcgccttg gcggaaaacc agtagttgtc
     1921 catctccagc ccaatgagcg cgtccttcca gccgcgatcg gcaatcagcc gggagagatg
     1981 ctccatcgga tggcgctcag tggattgcac ataatggtcg ggataacccg cgatattgtc
     2041 atggcccatg aacgccgtgc gcctggcgcc attggcatcc atgccgcgtc cccaccacac
     2101 cggctcacct tcgagcccca gcagcacggc ctgatgcaca tagaacgacc atccattata
     2161 gcccgtgagc cattccatgt ttgaggggtc ggagacaagg atcagatcaa gaccacgtgc
     2221 ctgcatggct ttgcgcgtct tggcaatgcg ccgctggtat tcggcgatgg ggaaatgcag
     2281 ggtggcctcg gtcatgccgc gagtttggcc cggcggtctg tttgacgcaa ggccagtgaa
     2341 atggctacgc ttggtccatg gctgacactt tcgacatcat cgtcatcggc gctgggattg
     2401 ccggcgcttc catcgccgcc caccttgccg aaaccaaacg tgtgctgatt ctggaaatgg
     2461 aggaccgtcc aggctttcac accaccggaa ggtccgccgc taccttcgag cccaattatg
     2521 gcccgccgcc catgctggcc ttcacccgcg ccagcaacat gttcttcacg aatccgccgc
     2581 agggcttcac cgacggcccc ttgctggtgc cgcgctcctc cctcttcttc gaagccgaag
     2641 gccaggaaaa cttcacgaaa aatctcctcg cctccggttc cggattggaa gaattgtcag
     2701 aaacccaagc ccgcaagctt ttccccaccc tccgcgaagg ctatgccaag cgcacctttc
     2761 tcgatcccca caccggcgat cttgatgtgg acctgctgca ccgcggctat ctcaagctgt
     2821 tcaaggcgcg cggcggccag ttgttgaaca gcgccgccgc tgaaaccatc ctgcgccaag
     2881 gccatgtctg ggaagtgatt tccccgcaag gaatcttcga agccgatatc ctcgtcaatg
     2941 cggcgggtgc ctggggcgat gccgtggcgg caaaggctgg cgtaaagccc ataggcctgc
     3001 agcccaagcg ccgctccatc ggcgtcatac cggtggaagg cattcccggc gtggccagct
     3061 ttcccatggt taccgacgtc ggcgaaacct ggtacgccaa gccgcaaagc ggcaagatga
     3121 tggtctcgtc tgccgatgcc acgccggttg atccccatga tgcctatgcc gatgacatgg
     3181 ccatagccga aggcatcgag cgcctgatga atgccaccac catagaggtc aacaacctcc
     3241 accacagctg gggcgggctg cgcaccttcg cccccgatgg cgcgcccgtg atcggatttg
     3301 atccgtccac ggaaggattc ttctggctcg tcggccaggg cggctacggc atccagtccg
     3361 ctcccgccct ctcccgcaca gccgccgcca tggtgctggg cgaagaacct ccgcaggatg
     3421 tcagggatgc aggattggtg cttgccgaaa ttctaccgga ccgcttctag gaaaccagca
     3481 ccggaaactc cggcccgtcc atcaccgcgc catgcgccag cgtcacgcca cgaaaaggcg
     3541 gtgaggtctt caccccttcg cgccgcgcaa accgcgcgtc atcacccagc agccgcagcg
     3601 aaaacgcccg gcgctgctcg ctcgccgaat tattggccgg cgcgccatgc agcgtcttgt
     3661 agttgaaggc caccgcatcg cccggctcca gcgcccagcc gatcacattg aagctgtccc
     3721 ggttgccgtt gatgtcgggc acttcctcaa gcccgtcatt ttcattgagc ggcgtcttgt
     3781 cgaagcgctc cggcctgaaa tacttgcccc acttgtgcga gccgccgaca aactccagcg
     3841 tccggtcgcg cggcactggg tccagcggaa tccagaggct caccgtgtcg ttgccgtcca
     3901 cgcagtaata gggctggtcc tggtgccagg gcgtggcaac cccggcactc gcctccttca
     3961 ccagcacatg ctcgtggaac agccgggccg ttttcgtgcc catcaattgc cgcccgatct
     4021 cggct
//
LOCUS       DAORVN010000093         3988 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_17698_length_3988_cov_3.310196, whole genome shotgun sequence.
ACCESSION   DAORVN010000093 DAORVN010000000
VERSION     DAORVN010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3988)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3988)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3988
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17698_length_3988_cov_3.310196"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="PKV23_02770"
     CDS             <1..214
                     /locus_tag="PKV23_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HQX84387.1"
                     /translation="HSADVAKVSVVGIGMRSHAGVAAKMFRSLADKGINIQAITTSEI
                     KVSVLIDEAYTELAVRALHQAYGLEA"
     gene            291..2555
                     /gene="ptsP"
                     /locus_tag="PKV23_02775"
     CDS             291..2555
                     /gene="ptsP"
                     /locus_tag="PKV23_02775"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012110656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HQX84388.1"
                     /translation="MAHSALGPRVLLRRLREVMAEPDTAQKRLDKIATLIAANMVAEV
                     CSIYVMRPGQVLELYATEGLKRGAVHKSKLKVGEGLVGLIGDQAIGLNLADAQNHPSF
                     KYLPETGEEIYHSFLGSPIMRGGTVIGVLVVQNRTRRHYSEEEEEALQTTAMVLAEVI
                     ASGELREVAREVAADVAHMRSHHLRGESLADGIALGHVVLHEPRVPIHNLIAESIPLE
                     KKRLEEAIQQLHLQVDQLLDGTESLRATDYSDVLETIRMFAHDKGWVNRLGEAVDTGL
                     TAEAAVERVQSDNRARMMRTPDPYLRERMHDLDDLSNRLMRILTGAVATASRGDLPAN
                     AIIVSRNMGPAELLDYDRKKIRGVILEEGGKTSHVAIVARALGIPAVGQAAGLIDLVD
                     TGSSIVIDGNTGELFVRPSADLQKSYAEKVRFYARKQAQYAVLRDVPAVTKDGVKINL
                     NINAGLIVDLPHLHDSGADGVGLYRTELHFMMASRFPRLTSQVRHYKAILDQAHGKPV
                     VFRTLDIGADKSLPYLRQPREDNPALGWRSIRMALDRPALLRLQVRSLLIAGQGHDLR
                     IMFPMIADVDEYLRARDIVDLEKAYLLARGHALPKSLQLGVMIEIPALIWQLDQLLPL
                     IDFASVGSNDLVQFLFASDRGNPRLAGRYDPLSPAALGAMRLIVEKAKAHGKPVTLCG
                     ELGGRPLEAMGLLGIGLTSVSMVPSGIGPVKAMLLKLDQGKLWSFMAPLLKSPAHSIR
                     GDLLEFAQRNGVPV"
     gene            2657..3766
                     /locus_tag="PKV23_02780"
     CDS             2657..3766
                     /locus_tag="PKV23_02780"
                     /inference="COORDINATES: protein motif:HMM:NF024855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4115 domain-containing protein"
                     /protein_id="HQX84389.1"
                     /translation="MDDPQLEFGRDETEYEAAGFREKPDGASDPAGEAGWFLQREREK
                     RGETLEEAGEATGIHPYHVEAIEFGDMTRMPTRMEALEMISAYAEYLGFDPEPLLHHY
                     IEFLPRPAIAPRTSHPANPAPLSSAKILSFGKMPKLMPLNIKLSDIPGGAGGLVASIA
                     GAIFIFAGASWMMIPLEEVAAPEAQVAIAADPMPTASTGPEAARVKITQETMPDDVLA
                     STDSTATDLSNPADADLGGLGAFIAKQVPEEQTAVPIPEPETATLDVPVVDDVMQTAE
                     GRVFGAKNETTRLVLKAKAPVWVRVEDAQGNVVMTQMLMKGDTYQVPDREGLVVIARD
                     GGLLSYVIDGKEKGILGTPGEILVGRSLNLSTFEG"
BASE COUNT          800 a         1190 c         1241 g          757 t
ORIGIN      
        1 gcattccgcc gacgtggcca aggtttcggt cgtcggcatc ggcatgcgga gccacgcggg
       61 cgttgccgcg aagatgttcc gctcgctggc cgacaagggc atcaacatcc aggcgatcac
      121 gacctcggaa atcaaagtca gtgttctgat tgatgaggcc tacacggagc tcgcggtgcg
      181 ggccctgcac caggcctatg gattggaagc ttgaagccca tgcacagcag cacccgtcaa
      241 aaatacatga cccgctgccg ctggaagcgg cagcgcgagg acacctgctg atggctcatt
      301 ccgcccttgg cccgcgtgtc ctgctccggc ggctccgcga ggtcatggcg gagccggaca
      361 cggcacaaaa gcggctggac aaaatcgcga ccctgatcgc ggccaacatg gtggccgagg
      421 tgtgctcgat ctatgtcatg cggccgggcc aggtgcttga gctctatgcc acggaaggcc
      481 tgaagcgcgg cgctgtgcac aagtccaagc tgaaagtggg cgagggcctg gtgggcctca
      541 tcggtgacca ggcgatcggc ctcaacctgg cggacgcgca aaaccacccg tcgttcaaat
      601 acctgccgga aaccggcgaa gaaatttacc actcgttcct cggctcgccc atcatgcggg
      661 gcggcacggt gattggcgtg ctggtggtgc aaaaccgcac gcggcggcat tattccgagg
      721 aggaggaaga ggcgctgcag accacggcca tggttctggc cgaagtcatt gcctcggggg
      781 aactgcgcga agtggcgcgc gaagtggcgg ctgacgttgc ccacatgcgc agccatcacc
      841 tgcgcggcga gtcgctggcg gacggcatag cgctcggcca tgtggttctg catgaaccgc
      901 gcgttcctat ccacaatctc atcgccgaaa gcatcccgct ggaaaagaag cggcttgaag
      961 aggctatcca gcagcttcac ctgcaggtcg accagttgct tgatggcacg gaatcgctgc
     1021 gcgccacgga ttattccgac gtgctcgaaa ccatccgcat gttcgcccac gacaagggct
     1081 gggtgaaccg gctgggcgag gccgtcgata cggggctcac cgccgaggca gcggtcgagc
     1141 gggtgcagag cgacaaccgg gcgcgcatga tgcgcacgcc cgatccctac ttgcgcgagc
     1201 gcatgcatga cctggatgat ctttcaaacc gcctgatgcg catcctgacc ggagcggttg
     1261 caaccgcctc acgcggcgac ctgccggcca atgcgatcat cgtgtcgcgc aacatgggcc
     1321 ccgccgaact tctggactat gaccgcaaaa agatccgcgg cgtcattctg gaggaaggcg
     1381 gaaagaccag ccatgtggcc attgtggcgc gggcccttgg cattccagcc gtcggccagg
     1441 ctgccggcct tattgacctt gtggatacgg gaagctccat cgtcattgac ggcaataccg
     1501 gcgagctgtt cgtcaggccg tcagccgacc tgcaaaagtc ctatgccgag aaggtgcgtt
     1561 tctacgcgcg caagcaggcg caatacgcgg tgctgcgcga tgtgccggcg gtcaccaagg
     1621 atggcgtcaa gatcaatctc aacatcaatg cgggattgat cgtcgatctg ccgcacctgc
     1681 atgattcagg cgccgacggc gtcggccttt accgcaccga actgcatttc atgatggcca
     1741 gcagattccc gcgtctcact tcgcaggtgc gccactacaa ggccattctc gatcaggcac
     1801 acggcaagcc ggtggtgttc cgcacgctcg atatcggcgc cgacaagtcc ctgccctact
     1861 tgcgtcagcc gcgggaagac aatccggcgc tcggctggcg ttccattcgc atggcgctgg
     1921 accggcccgc cctgctgcgc ctgcaggtac gctcactttt gattgccggg cagggccatg
     1981 atctcaggat catgttcccg atgatcgccg atgtggatga atacctgcgg gccagggaca
     2041 ttgttgatct ggaaaaggcc tatcttctgg cgcgggggca tgcgttgcca aagtcgctgc
     2101 agcttggcgt gatgattgag attcctgccc ttatctggca gcttgaccag cttctgccgc
     2161 tgatcgattt tgcctcggtc gggtccaatg acctggtgca gttcctcttt gcctcggatc
     2221 gcggcaatcc gaggcttgcc gggcgctatg atcccttaag cccggcagcg cttggcgcca
     2281 tgcggctgat cgttgaaaag gcaaaggccc acggcaagcc ggtcacactt tgcggcgaac
     2341 tcggcgggcg ccccctggaa gccatggggc tgctcggcat cgggctcaca tcggtttcca
     2401 tggttccctc tggcattggg ccggtgaagg cgatgctgct gaagctggac cagggcaagc
     2461 tttggtcctt tatggcgccg ctgctgaaaa gcccggccca ttcgatccgg ggcgatctcc
     2521 tggaatttgc ccagcgcaat ggcgttccgg tttaatttaa tcagggcgtt agcttgctga
     2581 taaccattct gcgtcattct ctcgaaaagc agcgttagta aaaagttaat gcttggtagc
     2641 acgagagagt aagaccatgg acgacccaca gcttgaattt ggccgtgacg aaacggaata
     2701 tgaagccgca ggcttccgtg agaaaccaga tggcgcaagc gatcccgcgg gggaagccgg
     2761 ctggtttttg cagcgcgagc gcgaaaagcg tggcgagacc ctggaggaag ccggcgaagc
     2821 cacgggcatt catccctacc atgtggaagc catcgaattc ggcgacatga cgcgcatgcc
     2881 gacgcgcatg gaagcgctcg agatgatttc ggcctatgcg gaatacctgg gttttgatcc
     2941 ggagccgctt ctccatcatt acattgaatt cctgccgcgg ccggcgatcg cgccgcgcac
     3001 cagccatccg gcaaatcccg cgcccctgtc gagcgccaaa attctgagct ttggaaaaat
     3061 gccaaagctg atgccgctga acatcaagct gtcggatatt cccggcggag caggcggtct
     3121 ggttgcatcc attgccggcg ccattttcat tttcgcaggc gcaagctgga tgatgattcc
     3181 cctggaagag gttgcagccc ctgaagcgca ggtggcgatt gccgccgatc cgatgccgac
     3241 ggcctccacg ggaccggagg ccgccagggt caagatcacg caagagacga tgcctgatga
     3301 tgttctggct tcgaccgaca gcactgccac ggacctgagc aatcccgcgg atgcggattt
     3361 gggcgggctt ggcgcattca ttgccaagca agtgcccgag gaacagacgg ctgttcccat
     3421 tcctgaacca gaaaccgcga cacttgatgt gcccgtggtc gacgacgtca tgcagaccgc
     3481 tgaagggcgc gtgttcggcg ccaagaacga aacgacacgc cttgtgctga aagccaaagc
     3541 cccggtttgg gtgcgggttg aagacgcgca aggcaatgtg gtgatgaccc agatgctgat
     3601 gaagggcgac acctaccagg tgccggaccg ggaaggcctt gtggtcatcg cgcgcgatgg
     3661 cgggcttctg agctatgtga tcgacggcaa ggagaagggc attctcggaa cgcccggtga
     3721 aatcctggtt ggccgctcgc tcaatctttc aaccttcgag ggctgacagc cactgcattt
     3781 gcggtggaag cagctttagt gcgtggcctg aaaccacgca ccaaaaccac agatcaatcg
     3841 catggacctg aagtgattac ttcagcattt cggtccacag agccgtgtag agctccgtca
     3901 cacgcggcgg acaggcggcg ataaacctgc ccttgtccgc cagttccggt ggaatgacaa
     3961 tttcacgagc cgccttcatg tcttcggg
//
LOCUS       DAORVN010000094         3984 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_17727_length_3984_cov_2.767116, whole genome shotgun sequence.
ACCESSION   DAORVN010000094 DAORVN010000000
VERSION     DAORVN010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3984)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3984)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3984
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17727_length_3984_cov_2.767116"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..302
                     /locus_tag="PKV23_02785"
     CDS             <1..302
                     /locus_tag="PKV23_02785"
                     /inference="COORDINATES: protein motif:HMM:NF024375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HQX84390.1"
                     /translation="RINQNAAWTSGKLFLMRIAPGTAMPEHGHGGEEITLILSGAYND
                     RFGRYARGDVADLDESIEHQPIVEKDSACICLVATEAPTRFKSWPARLLQPLIGI"
     gene            302..1051
                     /locus_tag="PKV23_02790"
     CDS             302..1051
                     /locus_tag="PKV23_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020178328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HQX84391.1"
                     /translation="MQALPWKVVWITGASTGIGREIALQLAGQGVTVAASARSADKLV
                     SLGPNIRPYPLDVTDRQAVLATLETIEGDLGPVDLAVMAAGTYSPLDVEKFDTKLFEA
                     AMTTNYLGVVNCLSGLLPRMFRRRRGHVAWIASVAGFVGLPKAAAYGPSKAALINLAE
                     SLKVDLERRGVTVSVVNPGFVETPLTAQNDFTMPFLMQAPEAARLTIEGLKRRRFEIS
                     YPWRFVLIMKLARLMPYGVFFRLIKRTVLKA"
     gene            complement(1116..2258)
                     /locus_tag="PKV23_02795"
     CDS             complement(1116..2258)
                     /locus_tag="PKV23_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018259902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HQX84392.1"
                     /translation="MAAYDLVLKGGHVVDPGQNLDAKMDVAFQAGKVAAIAKTIAVPA
                     GVEARDVSGLIVTPGLIDMHTHVYWGGTSLGIDAEDFCRKSAVTTAIDTGSAGPGNFA
                     GFRKHVIEPSAVRILAYLHISHAGIFGFSSRIMVGESEELRLMDPETAVEVATANCDL
                     IVGIKVRLGRWASGIHGMVPFEYALQVAEAAGMPMMVHIDDPPPSYVEVVERMRKGDV
                     LTHCFRPFPNTPTTADRRVRPEVLAARQRGVFFDIGHGMGSFSFKVARIMLENGFYPD
                     TISSDVHALCINGPAFDQVTTLSKFLHLGMPFSEVIRASTQNAALALSRPELGSLRPG
                     SVGDAAILSLDEGQFEFVDTVGEKVTGERKINVRGVVLGGKWWHSA"
     gene            complement(2320..2865)
                     /locus_tag="PKV23_02800"
     CDS             complement(2320..2865)
                     /locus_tag="PKV23_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019962558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HQX84393.1"
                     /translation="MIWPKMGLSRAWRYVVHRMARLKASPHAIALGFAAGAFASFTPF
                     VGLHFILAGLIAFALRSSILASAIGTVVGNPLTFPFIWLSTYNVGAALLGREAKGEVL
                     LDLPAVPESAVSAGMMEFAEKLFQRIEPVFLPMLIGGTLLGLACAAACYFIVRLSVER
                     FKQRRKSVRVRAAAPQQKATS"
     gene            complement(2941..>3984)
                     /locus_tag="PKV23_02805"
     CDS             complement(2941..>3984)
                     /locus_tag="PKV23_02805"
                     /inference="COORDINATES: protein motif:HMM:NF013832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX84394.1"
                     /translation="NAVKFDDPGTIEALTYWTMFIAIAAVIAVAGPYVSRNGDIIAEK
                     TNLSGGWIGLVLIATVTSLPELVTGVSAVTYWDLPDIAAGDALGSCVFNLAILIVVDF
                     MLRGEPVYSRANRGHIISGGFGIVLIGFVALTIMVDQNGGGLRLGHIGISTPIMLLLY
                     VGAMRTVFVYERDHREQFSEDVARRHPDVTLAMAARRYAAAAAAIAVAGVALPFAGSA
                     IADIMGWNRTFVGTLLIAGATSLPELVVTIAAVRYGALNMAVAGLLGSNLFNMLILAI
                     EDGLYLPGPLLAHVSPAHAVSAISAMIMTGIAIIGLQFGPRSRIFGTVGWASLAIFTV
                     YLFNSYVLYLHGH"
BASE COUNT          839 a         1272 c         1187 g          686 t
ORIGIN      
        1 ctcggataaa tcagaatgcg gcgtggacca gcggcaagct tttcctcatg cgcattgccc
       61 ccggcacggc aatgcccgag cacggccatg gcggcgaaga aatcaccctg atcctgtccg
      121 gcgcctacaa cgacaggttc gggcgctatg cgcggggcga cgttgccgat ctcgatgaga
      181 gcatcgaaca ccagcccatt gtcgaaaaag attcggcctg catctgcctg gtggcgaccg
      241 aagccccgac ccgtttcaaa tcctggccgg cccgcctgct gcagccgctg atcggaatct
      301 gatgcaggcc ttgccgtgga aagtcgtgtg gatcaccgga gcatccaccg ggatagggcg
      361 cgaaattgcg ttgcagcttg cgggccaggg cgtaaccgtg gccgcctcgg cccgcagcgc
      421 cgacaagctg gtttctctgg gtccgaacat caggccctat ccgctcgacg taactgaccg
      481 ccaggcagtc cttgccacgc tcgaaacaat cgaaggggat ttggggccag tggatttggc
      541 tgtcatggcg gcgggcacct acagcccgct tgatgtggaa aaattcgata ccaagctttt
      601 tgaagccgca atgaccacga actacctggg cgtggtgaac tgcctgtccg gcttgctgcc
      661 gcgcatgttc cggcgccgcc ggggccatgt ggcctggatt gcttcggttg caggcttcgt
      721 tggcctgccg aaggccgccg cctatgggcc ctccaaggca gccctcatca atctggcgga
      781 aagcctgaaa gtcgatctcg aacggcgggg cgtgacagtc agcgtggtca atcccggctt
      841 cgtggaaaca ccgctcaccg cgcagaacga tttcaccatg cccttcctga tgcaggcgcc
      901 ggaagcggcg cgcctgacaa tcgaagggct gaaacgccgc cgctttgaaa tttcctaccc
      961 ctggcgcttc gtgctcatca tgaagctggc gcgcctgatg ccttacgggg ttttcttccg
     1021 cctgatcaaa cgcaccgtgc tgaaggctta gcttgccgcc agaagccaat catcatggcg
     1081 ccaggctagt tcgccccgct gcccgatcac aaagatcagg cggaatgcca ccactttccg
     1141 cccagcacca cgccgcggac attgattttg cgttcgcccg ttaccttctc gcccaccgtg
     1201 tcgacgaact cgaactggcc ctcgtcgagg ctgagtatgg cggcatcccc caccgacccc
     1261 ggccttaggc tgcccagctc cggccgcgac agcgccagcg ccgcattctg ggtcgaggcc
     1321 cggatgacct cggaaaacgg catccccagg tggaggaatt tggagagcgt cgtcacctgg
     1381 tcaaaggccg ggccattgat gcaaagggca tggacatccg acgagatggt gtcgggatag
     1441 aacccgtttt ccagcatgat gcgcgccacc ttgaacgaaa acgaccccat gccgtggccg
     1501 atatcaaaga agaccccgcg ctggcgtgcc gcaagcactt ccgggcgcac cctgcggtcg
     1561 gctgtcgtgg gggtattggg aaaaggccgg aagcaatggg tgagcacatc gcccttgcgc
     1621 atccgctcca ccacctcaac gtaggacggc ggcggatcat cgatatgaac catcatcggc
     1681 atgcccgccg cttcggctac ctgcagggca tattcgaaag gcaccatgcc atggattccg
     1741 gacgcccagc gcccgagccg gaccttgatg ccgacaatca ggtcgcagtt ggccgtggcc
     1801 acttccaccg ccgtttccgg atccatcagc cgcagctctt cgctttcgcc caccatgatg
     1861 cggctggaaa agccgaaaat ccccgcgtgt gaaatatgga gataggccag gatcctcacc
     1921 gctgagggct cgatcacatg tttgcggaag ccggcaaaat tgccgggccc ggccgagccc
     1981 gtatcgatgg ccgtggtgac agcgctcttg cggcagaaat cctccgcatc gatgccaagc
     2041 gaggtgccgc cccaatagac atgggtgtgc atgtcaatga ggccgggagt gacgatcagg
     2101 cctgacacat cgcgcgcctc gacgcccgcc ggtaccgcaa tggttttcgc aatggccgcc
     2161 accttgcccg cctggaaggc cacatccatt ttcgcgtcaa ggttttgccc cggatcgacg
     2221 acatgcccgc ccttcaacac cagatcatac gccgccatgc tgcctccgat tacccatggc
     2281 gcaactggag acaattgccc ggatgatgtc aattccggct taggatgtgg ctttttgctg
     2341 tggcgcggcg gcacggaccc gcacggattt gcgccgctgc ttgaaacgct cgaccgacag
     2401 gcggacgata aaatagcagg cggcggcgca ggccagtcca agcagggttc cgccgatgag
     2461 catcggaagg aacacgggct cgatccgctg aaaaagcttc tcggcaaact ccatcatccc
     2521 ggcgcttacg gcactttcag gaaccgccgg caggtcaagc agcacctccc ccttcgcttc
     2581 ccgcccgagg agcgcggcac cgacattgta agtcgacagc cagatgaagg gaaaggtcag
     2641 cggattgccc acgactgtgc cgatggccga tgccaggatg ctggaacgga gcgcaaaggc
     2701 gataagcccg gccagaatga aatggaggcc gacaaagggc gtgaaagagg caaaggcgcc
     2761 agccgcgaaa ccaagcgcaa tcgcatgcgg gctggccttg aggcgcgcca tccggtgcac
     2821 gacatagcgc cacgcccggc tcaggcccat cttcggccag atcaagttgc gtacccttag
     2881 ccagaaccct ggcgacttgc ggcgcaaaaa catcccgtcc acttcgatcc gaaaaagctg
     2941 ctagtgccca tgtaggtata aaacatagga attaaataaa taaaccgtga aaatcgcaag
     3001 gctggcccac cccacggtgc caaatatccg cgaccgcggg ccgaattgca agccaatgat
     3061 cgcaatgccg gtcatgatca tcgccgagat cgccgagact gcgtgggctg gcgacacatg
     3121 cgccagaagc ggcccgggca ggtaaagccc atcctcgatg gccaatatca acatattgaa
     3181 gagattgctg ccgagaagcc ccgcaaccgc catgttcagg gccccgtagc gcacagccgc
     3241 gatggtcacg acgagttcgg gcaatgacgt ggcgccggcg atcaggaggg tgccgacaaa
     3301 ggtgcggttc cagcccatga tgtccgctat ggcgctgccg gcaaaaggca gggcgacgcc
     3361 tgcgacggcg attgcagcgg cagcggcggc gtaacggcgg gccgccatcg cgagggtcac
     3421 gtccggatgc cggcgcgcca cgtcctcgct gaactgctcg cgatggtccc gctcatagac
     3481 gaaaacggtg cgcatcgccc cgacatagag cagcagcatg atcggcgtcg aaatcccgat
     3541 atgccccagc cttaacccgc ccccgttctg atcgaccatg atggtcagcg caacaaatcc
     3601 gatcagcacg attccaaaac cgccggaaat gatgtgtccc cggttggcgc ggctgtaaac
     3661 cggttcgccg cgcagcatga aatcgaccac gatcaggatg gcaagattga agacgcaact
     3721 gccaagcgcg tcacctgcgg cgatgtctgg caggtcccag taggtcactg cgctgactcc
     3781 ggtcaccagt tccggcagcg atgtgacggt ggcaatcagg accagcccta tccagccgcc
     3841 ggagagattg gtcttctctg caatgatgtc gccgttgcgt gatacatagg ggcccgcaac
     3901 ggcaatcacc gcggcgatcg caatgaacat ggtccagtag gtcagcgcct cgatcgtgcc
     3961 cgggtcgtcg aacttgaccg cgtt
//
LOCUS       DAORVN010000095         3978 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_17778_length_3978_cov_2.953352, whole genome shotgun sequence.
ACCESSION   DAORVN010000095 DAORVN010000000
VERSION     DAORVN010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3978
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17778_length_3978_cov_2.953352"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..479
                     /locus_tag="PKV23_02810"
     CDS             <1..479
                     /locus_tag="PKV23_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018388226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HQX84395.1"
                     /translation="FKVWDSAVGKVGLGICWDQWFPEAARAMALMGAEILLYPTAIGS
                     EPGDPSYDSSNHWQNAMRGHAAANIMPLAASNRVGREAAPDGRADTFYGRSFIADCHG
                     EKLTEMDRVEEGVRLQSFDLAAIGELRRTWGVFRDRRPELYGSLLTIDGKLRFSAT"
     gene            531..953
                     /locus_tag="PKV23_02815"
     CDS             531..953
                     /locus_tag="PKV23_02815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX84396.1"
                     /translation="MEKPGFLIQHFMIAGLLAAIGVVVLAGQSQAGNRYCTYYPEDPN
                     CFDAIYGQGASPALGDEDGQFEDGTFSFQPPAPRPRAKSVTSCEIIGRALRRHGYRQV
                     RPVDCGGVDYKYVAYLGYQRYLLQVNARNGAIVHEINY"
     gene            complement(943..2385)
                     /locus_tag="PKV23_02820"
     CDS             complement(943..2385)
                     /locus_tag="PKV23_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013531377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HQX84397.1"
                     /translation="MTNVDLAYASATELLKLFKARKASPVELLKAQIARSGKLNKEIN
                     CLADCYFDEALVKAKAAEQLYAKRGFKPRALEGIPLLVKDAQRVKGKRTTHGSLIFAS
                     NIDDHSDPQIERLEKAGAIIFGRTTTPEFCLSGVTHSRIWGITRNPWNLDYGPGGSSG
                     GSGAALAAGFTTLATGTDIGGSIRIPASACGVVGFKPPHGRNPDGPPGNFDRFNHCGP
                     MTRTIADAALMQNTTSGPHPLDHDSLRNRVRLPVKAESIRGMKIAYSLDFGYVEVDPE
                     VRRNTLAALAVFRGLGAEVNEVDLGWTPQVDLDCFHWYNAMHFGRQTIWHREKNARLM
                     TDYALKTADAVERNTGINDVHKAWERIHTMYQTLGPILAAHDVLICPTNNLAAVKADH
                     DPWDQAFTVNGKKADAEYGWIMTAQFNMLHNCPVLAVPSGHTKQGIPTGIQIVGRTWD
                     DARVFRAGLAYEKALGGWYTAKEKRPVLNN"
     gene            2446..3189
                     /locus_tag="PKV23_02825"
     CDS             2446..3189
                     /locus_tag="PKV23_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009364769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQX84398.1"
                     /translation="MAGKLEGRVALVTSAGQGIGRASAIALAREGAKVFATDIREELF
                     MNISKEHPGITGFKLNVLEQAEVDAALKRTGPIDILFNCSGFVHNGTVLDCEEKDWDF
                     SFDLNVKAHYRMIKAYLPGMLAKGKGNVINMASVASSVKGAPNRFVYGASKAAVIGLT
                     KALAIDFVSKGIRCNCICPGTVETPSLYDRMRAQGDVEKAKAAFIARQPMGRMAQPEE
                     IAEMVVYLASEDTAFITGQALVIDGGWSI"
     gene            3281..>3978
                     /locus_tag="PKV23_02830"
     CDS             3281..>3978
                     /locus_tag="PKV23_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006338817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQX84399.1"
                     /translation="MAIGKTAIEAVGVTKIFQSSGQDSLKALDNVSVTIGENEFFTLL
                     GPSGCGKTTLLRLIAGFDFPTDGQILLHGKDIAPLPPFQRPVNTVFQNYALFPHMTVG
                     QNIGFGLEMQGKPKAQVEARVAEMLRLVQMEELKNRRTSQISGGQQQRVALARALAPE
                     PKVLLLDEPLSALDYKLRKEMQIELKRLQNETGITFIFVTHDQEEALTMSDRIAVMSK
                     GQILQVGSPRDIYDR"
BASE COUNT          810 a         1104 c         1244 g          820 t
ORIGIN      
        1 ggttcaaggt ctgggactcg gcggtgggca aggtggggct tggcatatgc tgggaccagt
       61 ggtttccgga agcggcgcgc gccatggcgc tgatgggggc cgaaatcctg ctctatccca
      121 cggccatcgg ttcggaaccc ggcgatcctt cctatgattc cagcaatcac tggcagaacg
      181 ccatgcgggg ccatgcggcg gccaatatca tgccgctggc ggcctccaac cgggtgggcc
      241 gggaagcagc gcccgacgga cgggcggaca cattctatgg ccggtccttc attgccgact
      301 gtcatggcga aaaattaacc gagatggatc gcgtcgaaga aggggtgcgg ctgcagagtt
      361 ttgacctggc ggccattggc gaattgcgcc ggacctgggg ggtgttccgc gaccggaggc
      421 ccgaacttta tggcagtctg ttgacaatcg atggtaaatt gcgcttttcc gccacatagt
      481 tagctggatt gcgacaatct gacgccgcaa tccccgccta gcctggcttc atggagaaac
      541 cgggttttct cattcagcac ttcatgattg cgggtctgct tgccgccatt ggcgtcgtcg
      601 tccttgcggg ccagtcccag gctggcaacc gctattgcac ctactatccg gaagacccca
      661 actgcttcga tgcgatttat ggccagggcg cttcgccggc gctgggcgat gaagacgggc
      721 aattcgagga tggtacattc agctttcagc cacctgcgcc ccgcccgcgg gcgaagtccg
      781 tcacatcttg cgaaatcatt ggccgggcct tgcgccggca cggttaccgc caggtgcgcc
      841 cggtggattg cggcggcgtt gattacaagt atgtggccta cctcggttac cagcgctacc
      901 tgctgcaggt gaacgcccgc aacggcgcga tcgttcacga aatcaattat tgagcaccgg
      961 gcgcttttcc ttagccgtgt accagccgcc caaggccttt tcataggcca ggccggcgcg
     1021 gaaaacgcgg gcgtcatccc aggttcggcc gacgatctga attccggtgg gaatgccttg
     1081 cttcgtatgg ccggagggaa cggccagcac cgggcaattg tgcagcatgt tgaactgcgc
     1141 ggtcatgatc caaccgtatt cggcatcggc cttcttgccg ttgacggtga aggcctggtc
     1201 ccagggatca tggtcggctt tcacggcggc gagattgttg gtggggcaga tcaaaacatc
     1261 atgggcagca aggattggcc ccagtgtctg atacatggtg tggatgcgct cccacgcctt
     1321 gtggacatca ttgatccccg tattgcgttc cacggcatcc gccgttttca gggcatagtc
     1381 ggtcatcagg cgggcattct tttcgcggtg ccaaatcgtc tggcggccga aatgcatggc
     1441 gttgtaccaa tggaagcaat cgaggtcgac ttgcggcgtc cagccgagat cgacttcatt
     1501 gacctcggcg ccgaggccgc gaaacacggc aagtgccgcc agggtattcc tgcgcacttc
     1561 cggatccact tcgacataac cgaagtcgag cgaataggcg atcttcatgc ccctgatgct
     1621 ttcggccttc acgggaagcc tcacacggtt gcggagcgag tcgtgatcga gggggtgcgg
     1681 ccccgacgtt gtgttctgca tcagggccgc atcggcaatg gtccgggtca taggaccgca
     1741 atggttgaaa cggtcaaaat ttccgggcgg gccatcggga ttgcggccgt gcggcggctt
     1801 gaagcccacg acgccacagg cagacgcggg gatgcggatg gagcctccga tgtcggtgcc
     1861 ggtggcaagg gttgtgaagc ctgcggccaa tgccgcgccg gaaccgccgg aagagcctcc
     1921 tgggccataa tcgaggttcc aggggtttcg cgtgatgccc catatgcgtg aatgggtgac
     1981 accggagagg caaaactccg gcgttgtggt gcggccaaaa atgatggcgc cggctttttc
     2041 cagacgctcg atctggggat cggaatgatc atcaatgttg ctggcgaaaa tcaaagagcc
     2101 atgggtggtg cgtttgcctt tgacccgctg ggcatccttg accagcaagg gaatgccttc
     2161 gagggcgcgc ggcttgaagc cgcgtttcgc gtaaagctgc tctgccgctt ttgccttgac
     2221 gagggcttca tcgaaatagc agtcggcgag gcagtttatc tctttgttca gtttcccgct
     2281 gcgcgcgatc tgcgctttca gcagctcaac gggcgaagcc ttgcgggcct tgaacagctt
     2341 cagaagctcc gttgcagaag cataggccag atcaacattg gtcatggaca atttccacgg
     2401 attagagtta ggcaggagtt tgaaccataa tgagggaact agtccatggc cgggaagctt
     2461 gagggtcgcg ttgcattggt cacttcggcg gggcagggca ttggccgggc ctcggccatt
     2521 gcgctggcgc gggagggggc aaaggttttt gccaccgaca tccgggaaga gctgttcatg
     2581 aacatttcca aggaacaccc cggcattacc ggctttaaac tcaatgttct ggagcaggcg
     2641 gaggtggatg cggctttaaa gcgcaccggc cccatcgaca tcctgttcaa ctgctcaggc
     2701 tttgtgcaca acggcacggt gctggactgc gaagagaagg actgggactt ctcctttgac
     2761 ctgaacgtca aggcccatta ccggatgatc aaggcctatc tcccgggcat gctggcaaag
     2821 ggcaagggca atgtcatcaa catggcatcg gttgcctctt cggtgaaggg tgctcccaac
     2881 cgctttgtct atggcgccag caaagccgcg gttatcgggc ttaccaaggc cctggcgata
     2941 gatttcgtga gcaagggcat ccgctgcaat tgcatttgcc cgggaacggt ggaaacgcca
     3001 tcgctttacg accggatgcg ggcccagggg gatgttgaaa aggcgaaagc ggcctttatc
     3061 gcacgccaac ccatggggcg catggcgcag cctgaagaaa tagcagagat ggttgtttat
     3121 ctggcaagcg aggacacggc cttcatcacc gggcaggcgc ttgtcatcga tggcggctgg
     3181 tcgatctaat ccttcgcaac caccccattg gcaagccgaa aaattcccca taggatagta
     3241 ttttaatcgt ctggaacaag acgaagggga gaaagctaaa ttggcaattg gcaagactgc
     3301 cattgaggca gtcggcgtta caaagatatt ccaaagctcg ggccaggaca gcctcaaggc
     3361 gctggacaat gtgtccgtca cgattggcga aaacgagttc tttacccttc tcggcccttc
     3421 gggctgcggg aaaaccaccc tgttgcggct gattgccgga tttgatttcc caaccgacgg
     3481 gcaaatcctg ctgcatggga aagacattgc gccactgccg ccatttcagc ggccggtcaa
     3541 cacggtgttt caaaattacg cgctgttccc gcacatgaca gttggccaga acatcggttt
     3601 cggacttgag atgcaaggca agccgaaggc gcaggttgag gcgcgggtcg ccgagatgct
     3661 gcgcctggtg caaatggaag aactcaagaa ccggcggacc agccagattt ccggcggcca
     3721 gcagcagcgc gtggcgctgg cgagagccct ggcgccggag cccaaggttc tgcttctcga
     3781 cgaaccgctt tccgcccttg actacaagtt gcgcaaggaa atgcagatcg agctgaagcg
     3841 gctgcagaac gagacgggca tcaccttcat cttcgtgacg catgaccagg aagaagcgct
     3901 gaccatgtcg gaccgcattg cggtgatgtc gaagggccaa attcttcaag tgggatcccc
     3961 ccgcgatatc tatgaccg
//
LOCUS       DAORVN010000096         3977 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_17789_length_3977_cov_3.083631, whole genome shotgun sequence.
ACCESSION   DAORVN010000096 DAORVN010000000
VERSION     DAORVN010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3977
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17789_length_3977_cov_3.083631"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..341)
                     /locus_tag="PKV23_02835"
     CDS             complement(<1..341)
                     /locus_tag="PKV23_02835"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007603754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HQX84400.1"
                     /translation="MSKLYGESHRKLQREFDTERLADRIEQRLFHEHITPDDRAFIER
                     MDMFFLATADREGRPNCSYKGGDPGFIRVLDSKTLAFPNYDGNGMYLSTGNTLENPAV
                     GLLFIDFENRKR"
     gene            complement(458..769)
                     /locus_tag="PKV23_02840"
     CDS             complement(458..769)
                     /locus_tag="PKV23_02840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX84401.1"
                     /translation="MKTVEQLLNHAKALLPRRYSPSETLSELAAGALVIDIRGDEQQR
                     ADGLIPGTLVIRRNVLEWRCDPVSPWRHPSVTNHQQKIILFCNEGYSRFWPPQIFRYW
                     D"
     gene            complement(766..1200)
                     /locus_tag="PKV23_02845"
     CDS             complement(766..1200)
                     /locus_tag="PKV23_02845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX84402.1"
                     /translation="MGTAVAIALVLFCACAAQAHSYRIGNLEIVHPAIMVPQANSDCT
                     CAHVTITNHGSETEYFLGADIEAASSTRLLHVASRGLNISAPSIVAIAPGAKLDLHRH
                     EWCLFLSDIPKGLEPDIGAYPARLMFRHAGTIAIEFMVDANQ"
     gene            complement(1253..1795)
                     /locus_tag="PKV23_02850"
     CDS             complement(1253..1795)
                     /locus_tag="PKV23_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018237411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX84403.1"
                     /translation="MKKFVLLTASLCTFCLAAASVTRADDAELIKSAESASPAAVGAG
                     ATIYAPQADGSMKTLREGTNGFWCMPDNPASPGPDPMCGDANAMEWAMAWIGKKDPPK
                     GKVAMIYMLAGGTDASNTDPFAMAPTEGNNWVKTGRHIMIMNAMDMMAGYPTDAKPDT
                     SRPYVMWSRTPYQHLMVPVE"
     gene            1955..2536
                     /locus_tag="PKV23_02855"
     CDS             1955..2536
                     /locus_tag="PKV23_02855"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HQX84404.1"
                     /translation="MDQKTDQVHTSQQILDIAQRLVQTRGFNAFSYADIASELKVSKA
                     SLHYHFASKAELGIKLIERYEAGMERALHEIDASGGGAATKLRRYVNIYASVLADERM
                     CLCGMLAAEYVTLPKAMQTALDSFFALNERWLAAILEDGRAGGTLQFKGKASEAADYI
                     IGSLEGSMMMARSHGGTERFDAASRRLLAEFGI"
     gene            2593..3537
                     /locus_tag="PKV23_02860"
     CDS             2593..3537
                     /locus_tag="PKV23_02860"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HQX84405.1"
                     /translation="MEVKAAICQRPPVLLDKKASIAAALAMVAEAAGQGARLMTFPEA
                     FLPGYPTWVWRLRPGADMALGNEIHRQLQLNAIDAASGDLDPLRDAAARHGMVIVMGM
                     HELDSTYSGSTLFNTVVAIGDDGAILNTHRKLMPTNPERMVWGAGDASGLRVVDTKFG
                     RLGCLLCWENYMPLARYALYAQQMDILVALTWDCGDLWTASMRHIAKEGGCWVLSSAT
                     ALQGSDMPASFPGRGQLFKDAEWINPGDAIAVKPGGTLAAGPLHEEKAILYVTIDADM
                     ARAARKSLDVAGHYSRSDVFQLNVDRRPKPPAVFRDSD"
     gene            complement(3556..>3977)
                     /locus_tag="PKV23_02865"
     CDS             complement(3556..>3977)
                     /locus_tag="PKV23_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008839735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQX84406.1"
                     /translation="MTMVVWRGIKDNPWLGHAKEVVLKFLPPPGENALSCGPGPFSMA
                     DTGVVTKQLEIAGYTGIEFEQVDAELFVGKDVDDAVAFQLALGPAGEVYREAGELAEK
                     RHDEIAAALRVELAKYQRPNGIIMDSSSWKVTARNPG"
BASE COUNT          809 a         1099 c         1231 g          838 t
ORIGIN      
        1 cgcttgcggt tttcgaaatc gatgaagagc aagcccaccg ccggattttc cagggtgttg
       61 ccggtggaga gatacatgcc gttgccgtcg taattcggga aggccagggt cttggaatcg
      121 aggacacgga tgaagccggg atcgccgccc ttgtaggaac agttggggcg gccctccctg
      181 tcggcggtgg cgaggaaaaa catgtccatg cgttcgatga aggcccggtc gtcgggcgtg
      241 atatgctcgt ggaacaggcg ctgctcgatc cggtcggcga gccgttcggt gtcaaactcg
      301 cgctgcagct tgcggtggct ttcgccatag agcttcgaca tggcattcct cgctgcgggc
      361 tggagagatc aactcatttt atctagatca tagggcttgg tcggcaagcc tgaggccttc
      421 cagccggtga agccgctctc catgtctgat gcaaagatca gtcccaatat ctgaagattt
      481 gcggcggcca gaaccgactg tagccctcgt tgcagaacag gatgatcttc tgctggtgat
      541 ttgtcacgct gggatggcgc cagggcgaga cgggatcgca acgccactcc agcacgttgc
      601 ggcgaatgac cagggtgccg ggaatgaggc catcggcgcg ctgctgctcg tcgccgcgga
      661 tgtcgatgac cagggcgccg gccgcgagtt cggacagggt ttccgacggc gaatacctgc
      721 gcgggagcag cgcctttgcg tgattgagca gttgttcaac cgtcttcatt gatttgcgtc
      781 aaccatgaat tcaatggcga tggttccggc gtggcgaaac atcagtcgcg ccggataggc
      841 accaatgtcg ggctcgaggc ctttgggaat atcgctgaga aacagacacc attcgtgacg
      901 gtgaaggtca agcttggccc ccggcgcaat cgccacgatg gaaggcgcgc tgatattgag
      961 cccgcggctt gcgacatgaa gaagccttgt actgctcgct gcctcaatgt ccgccccgag
     1021 aaaatattcg gtctcggaac catggttggt gatcgttaca tgggcacagg tgcaatcgga
     1081 gttagcctgc ggcaccatta tggccggatg aacaatttcc agattgccaa tgcggtagga
     1141 atgcgcctgc gctgcgcagg cacaaaataa cacgagagcg attgctactg ccgttcccat
     1201 ggaaaaaacc catggcgcag gaacagtgaa tatgtccctg cgcccggaca ctttattcca
     1261 ccggaaccat gagatgctgg tacggcgttc tggaccacat cacataaggc ctgctggtat
     1321 cgggttttgc atcggtgggg tagccggcca tcatgtccat ggcattcatg atcatgatat
     1381 gacgccccgt cttgacccag ttgttgcctt cggtcggcgc cattgcaaat ggatcggtgt
     1441 tgctggcatc ggtgccgccg gcaagcatgt agatcatggc caccttgcct tttggcggat
     1501 cttttttgcc aatccaggcc atggcccatt ccatggcatt ggcgtcgccg cacatcggat
     1561 ccggtccggg tgaggcggga ttgtccggca tgcaccagaa accgttggtt ccctcgcgca
     1621 gagtcttcat agaaccgtcg gcctggggag catagatcgt ggcgcctgcc cctacggcgg
     1681 ctgggctggc ggattcggcg cttttgatca attcggcatc gtcagcccgg gtgaccgatg
     1741 cggcagcaag gcagaatgta cataggctag ctgtcagcaa gacaaacttc ttcatgatac
     1801 ttctccctag attgtgggca gtttggaatc gctcacttaa cctgtgctta cctattgata
     1861 agtaacttgt caactagtag atagaatcgt atgagggcgg gagcggacgc gattcgacaa
     1921 atgccatttt tccaaaggat ttccaggccg gcacatggat cagaaaacag accaggttca
     1981 cacttcacaa cagatactgg atattgccca gcggctggtt caaacccgcg ggttcaatgc
     2041 tttcagctat gccgatattg ccagcgagtt gaaggtttcg aaggccagcc tgcactacca
     2101 cttcgcttca aaggccgaac tcggcatcaa gctcatcgaa cgctatgaag ctggaatgga
     2161 gcgggctctt catgaaattg atgccagtgg cggcggggca gcgacgaagc tgaggcgcta
     2221 tgtgaatatc tatgcaagcg tcctggcaga tgaaaggatg tgcctgtgcg gaatgctggc
     2281 tgcagaatat gtcacgcttc caaaagccat gcagacggcg ctcgacagtt tttttgcgtt
     2341 aaacgagcgc tggctcgcgg caattcttga agatggccgc gccggcggca cgttgcagtt
     2401 caagggaaag gcttccgagg cagcggacta tattatcggc tctcttgaag gttcgatgat
     2461 gatggcgcgt tcgcatggcg gcacggagcg ttttgacgcc gcatcaagac ggcttctcgc
     2521 cgagttcgga atttgatgga gagcgcgggc ccgattgcat aggcagccgg gacacgctac
     2581 gggattcata tcatggaagt caaagccgca atctgccaac ggccccccgt cctgctggac
     2641 aaaaaggcct cgatagcagc ggcgctggcg atggtggcag aagcggctgg ccagggcgcc
     2701 aggttgatga cttttcccga ggcgtttctt ccgggttatc ccacctgggt gtggcggttg
     2761 cgccccggag ccgacatggc gctgggaaac gagattcatc gccagctgca gctgaacgca
     2821 atcgacgccg catccggcga ccttgatccg cttcgcgacg cggccgcaag acatggcatg
     2881 gtgatcgtca tgggcatgca tgagctcgac agcacgtaca gcggttcaac gcttttcaac
     2941 acggtggtgg ccatcggaga tgacggcgca atcctgaaca cgcaccgcaa gctgatgccc
     3001 accaatccgg aacgcatggt gtggggtgcg ggtgatgcaa gcggcttgcg cgtggttgac
     3061 acgaagttcg gccgactggg ctgcctgctg tgctgggaaa actacatgcc gctcgcccgt
     3121 tacgcgcttt atgcacagca gatggacatt ctggtggcgt tgacctggga ttgcggcgac
     3181 ctgtggactg ccagcatgcg gcatatcgca aaggaaggcg gctgctgggt gctgtcatcg
     3241 gccactgcgc tgcagggttc agatatgcct gcgtcattcc cgggccgcgg ccagctcttc
     3301 aaggatgcgg aatggatcaa tccgggtgat gccattgccg tcaagcccgg tggcacgctc
     3361 gcagccgggc cgctgcatga agagaaggcg atcctctatg tcacgatcga cgctgacatg
     3421 gcccgcgccg cgcgcaagtc gctggatgtt gcgggccact attcccgttc ggacgtgttt
     3481 caactgaacg tggaccggcg gccaaagccg ccggcggtat tccgggattc cgactagcgc
     3541 cgacgcaatg cgagattatc caggattgcg ggccgtgacc ttccatgagc ttgaatccat
     3601 gatgatgcca ttggggcgct ggtatttggc gagctccacc ctgagtgccg cggcaatctc
     3661 gtcgtggcgc ttttcagcca gttcgccggc ctcgcggtaa acttcccccg ccgggccgag
     3721 ggcgagctgg aaggcaaccg cgtcatcaac atccttgccc acgaacagct ccgcatcaac
     3781 ctgttcaaac tcgatgccgg tgtagccggc gatttccagc tgcttggtga cgacacccgt
     3841 gtccgccatg gagaatgggc cagggccgca actcaaggca ttttcaccgg gcggcgggag
     3901 gaatttcaga acgacttcct tggcgtggcc gagccaggga ttatccttga tgccgcgcca
     3961 taccaccatg gtcatga
//
LOCUS       DAORVN010000097         3974 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_17807_length_3974_cov_4.412095, whole genome shotgun sequence.
ACCESSION   DAORVN010000097 DAORVN010000000
VERSION     DAORVN010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3974)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3974)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3974
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17807_length_3974_cov_4.412095"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            14..547
                     /locus_tag="PKV23_02870"
     CDS             14..547
                     /locus_tag="PKV23_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018448710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQX84407.1"
                     /translation="MTSRPVLRDASLPEGYSLRPVDVSDLDGYRRIFRKVGEDLMWFS
                     RLIMPDEKLAAILGNPQVESYVLYQGNAAIGILELDFKDMPNCELAFFGLAKEAIGSG
                     LGRVLMNIAITKAWEKPITRLWVHTCHFDHPNALGFYKRSGFEPYSLMVEIHDDPRLA
                     GKLPRHASPHVALLDPR"
     gene            680..2545
                     /locus_tag="PKV23_02875"
     CDS             680..2545
                     /locus_tag="PKV23_02875"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011471354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HQX84408.1"
                     /translation="MPFPQTPAPFTRALTERKYDEPTPVQLAVLEARAEGRDLVVSAQ
                     TGSGKTVAYGLAIATTLLGEAERFQQDASPMALIVAPTRELAMQVERELEWLYHYTGA
                     RILSCVGGMDPKRERRRLADGAHIIVGTPGRLRDHLERGVLNITNLKAVVLDEADEML
                     DLGFREDLEFILEATPAERRTLLFSATMPKIIANLAKRYQRNALRIEASGGERAHADI
                     EYRAMRVAPNEVEHAVVNVLRFVEAQSTLVFCNTRDHVRHLHATLLERGFAAVYLSGE
                     LSQGERNHALQALRDGRARVCVATDVAARGIDLPNLALVIHADLPNDAEILQHRSGRT
                     GRAGKKGVSVVLVPLSRRRKAEQLLAAAKVNPVWSGPPQAEDIRRLDQQRLLHDPLLT
                     EVSSEEDLEMARAVLAMRSPEEVAAALVRVYRARLPAAEDVFDPGPGREFREAKPARR
                     DAHSAEPRNFTWFRLDIGRKNNADPKWLLPMICRRGKVVKADIGAIRITDNETRFEIA
                     NAVVDRFSIASRVADDEDVKIELLVGNPEISRPSRNPDRKPNFKPPHAAEPSRYEDRR
                     PHDGPAKPKFEATGYKGKKSFAGGKPRFEGTGKPHAAGNTPAFKEKKAAWKKKKR"
     gene            complement(2542..3456)
                     /locus_tag="PKV23_02880"
     CDS             complement(2542..3456)
                     /locus_tag="PKV23_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018328001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HQX84409.1"
                     /translation="MPHKIVVAALYKFVTLKDFASLRQPLLAAMKAHGVKGTLLLAEE
                     GINGTISGSRSAIDGFLAVLRSDARLADIDHKESYCDKDPFYRSKVKLKKEIVTIGVV
                     GVDPNKQAGTYVSPVDWNTLIADPDVLLIDTRNDYEVALGTFEGAADPGTSSFGEFPK
                     FVSENLDPAQHRKVAMFCTGGIRCEKASSYMQSQGFREVYHLKGGILKYLEEVPKEQS
                     KWQGDCFVFDHRVAVGQGLVPSEIELCHGCRMPLDPADRFSEFYEEGVSCPKCFATLS
                     ERSKASARERQKQIDLAKKRNLRKPIGA"
     gene            3544..>3974
                     /locus_tag="PKV23_02885"
     CDS             3544..>3974
                     /locus_tag="PKV23_02885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX84410.1"
                     /translation="MPEFILNAGVEIPFILILLALLGFRRKVNPRAKLLIRAPQEKVF
                     ALVDLHHGKVQTWSRTTVTSELLDDARQVFRMTYVTTLSTGAQQKSQAHFRVAERRPP
                     AYLELHRDGLEGKSLNNELLKIVTETEAHPEGTWLTQKYKWG"
BASE COUNT          818 a         1271 c         1091 g          794 t
ORIGIN      
        1 gacctgcctt gagatgacgt ccagaccagt ccttagggat gcgtctctgc cggaaggcta
       61 ttctttgcgg ccggttgatg tgagcgacct cgatggctac cgcagaatat tccgcaaggt
      121 gggcgaagac ctcatgtggt tttcacgcct catcatgccc gatgaaaagc tggcggccat
      181 tctcggcaac ccgcaggtgg aatcctatgt cttgtatcaa ggcaacgcgg cgatcggaat
      241 tctggaactg gatttcaagg atatgcccaa ctgcgagctc gccttctttg gcctggccaa
      301 ggaagccatt ggcagcggcc tgggccgtgt actgatgaat atcgccatca ccaaggcctg
      361 ggaaaagccg atcacccgtt tgtgggtcca cacctgccat tttgaccacc ccaacgcgtt
      421 gggcttttac aagcgctcag gctttgagcc ttatagcctc atggtcgaaa ttcacgacga
      481 cccccgtctc gccggcaagc tgccgcgcca cgccagcccc catgtggcgt tgctcgatcc
      541 gcgatagtgg ttaagggcga agtttgcgtc gccatggtgt ttccagtgaa cttgctctaa
      601 gagcggccca attggccctg cgtagtagcg gcatccccgt ttgcccgccc tcaactccgt
      661 tagaaaagta acctttaaat tgccctttcc ccagacccct gccccgttca cccgagccct
      721 caccgagcgc aagtatgatg agcccacccc ggttcagctt gccgttctcg aagccagggc
      781 ggaggggcgc gatctcgtcg tttcggccca aaccggctcc ggcaagaccg tggcctatgg
      841 ccttgccatc gccacgacgc tgcttggcga agccgagcgc ttccagcagg atgcctcgcc
      901 aatggccctc attgtggcgc caacccgcga actcgccatg caggtggagc gcgaactgga
      961 gtggctctac cattataccg gggcccgcat cctttcctgc gtcggcggca tggaccccaa
     1021 gcgcgagcgc cgcaggcttg ccgatggcgc ccacattatt gtgggaacac cgggacgcct
     1081 gcgtgaccat ctcgagcgcg gcgttttgaa tatcaccaat ctcaaagccg tggtcctcga
     1141 cgaagccgat gaaatgcttg atctcggctt tcgcgaggac ctcgaattca tcctggaagc
     1201 aacgcccgcc gaacggcgca cccttttgtt ttccgcgacc atgcccaaaa tcattgccaa
     1261 cctggccaag cgctaccagc gcaatgccct gcgcatcgag gcctcaggcg gcgaacgggc
     1321 ccatgccgac atcgaatacc gcgccatgcg cgttgccccc aacgaggttg agcatgcggt
     1381 cgtcaatgtg ctgcgctttg tcgaggcgca aagcaccctt gtgttttgca acacccgcga
     1441 ccatgtccgc catttgcatg caaccctgtt ggagcgcggc tttgccgccg tctatctctc
     1501 cggcgaactc agccagggcg aacgcaacca tgccctgcag gcgctgcgcg acggccgggc
     1561 ccgcgtctgc gtcgcaaccg atgtggcggc ccgcggcatt gaccttccca accttgccct
     1621 cgtcattcat gcggatctgc ccaatgacgc ggaaatcctg cagcaccgca gcggtcgcac
     1681 cggacgcgcc ggcaagaagg gcgtgagcgt cgtgctggta cccctgtcaa ggcgccgcaa
     1741 ggccgaacag ctgctcgcgg cagccaaggt gaatcccgtg tggagcggtc cgccccaggc
     1801 agaagacatt cgcaggcttg accagcagcg tctgctgcat gatccgctcc tcaccgaggt
     1861 cagcagcgaa gaggacctgg aaatggcccg cgccgttctg gccatgcgct cgccggaaga
     1921 agttgccgcg gcccttgtcc gtgtttaccg cgccagactg cctgccgctg aggatgtgtt
     1981 tgaccccggc cctggccgcg agttccgcga ggcaaaaccg gcgcgccgtg atgcccattc
     2041 agccgaaccc cgcaatttca cctggttccg ccttgacatc ggccgcaaga acaatgccga
     2101 tccgaaatgg ctgctgccga tgatctgccg ccgcggcaag gtagtaaagg ccgacatcgg
     2161 cgccatccgc atcaccgaca atgaaacccg gtttgaaatt gccaatgcgg ttgtcgaccg
     2221 tttctccatc gccagccggg ttgccgatga cgaggacgtc aagattgaat tgctggtcgg
     2281 caatccggaa atcagccgtc cttcgcgcaa tccggaccgc aagccgaact tcaagccgcc
     2341 ccacgctgct gaaccatcgc gctatgaaga cagaagaccc catgatggcc ctgccaagcc
     2401 gaaattcgaa gcaaccggct acaagggcaa gaaatctttt gcaggcggca agccgagatt
     2461 tgaagggacg ggaaaacccc acgctgcagg caacacaccg gctttcaagg agaagaaagc
     2521 cgcctggaaa aagaagaaac gctaggctcc gatcggtttg cgcaggttcc gcttcttcgc
     2581 caggtcaatc tgcttctgcc gttctcgtgc cgaagccttc gacctctcgc taagtgtcgc
     2641 aaaacacttc ggacagctca cgccctcttc atagaattcc gaaaacctgt cggcaggatc
     2701 cagcggcatg cggcagccgt ggcataattc aatttcgctg ggaaccaggc cctggcccac
     2761 cgccacccgg tggtcgaaaa caaaacagtc accctgccat ttgctttgct ctttcggcac
     2821 ctcctcaaga tatttgagaa tgccgccttt caggtgatac acttcgcgga agccctgcga
     2881 ctgcatgtag ctcgaggcct tctcgcagcg gatgccgccg gtgcagaaca tggccacttt
     2941 ccggtgctgc gccggatcaa gattctcgct gacgaatttg ggaaattcgc caaaactgga
     3001 ggtcccggga tcagccgctc cctcgaacgt gccaagcgcc acttcataat cgttgcgcgt
     3061 atcaatgagc aggacatcgg ggtcggcgat cagcgtattc cagtccaccg ggctcacata
     3121 ggtgccggcc tgtttgttcg ggtctacgcc gacaactcca atcgtgacaa tttccttctt
     3181 cagcttcacc ttgctgcgat agaacggatc tttgtcgcaa taggattcct tgtggtcaat
     3241 gtcggcaagg cgggcgtcgg atctaagcac tgcaagaaat ccgtcgatgg cacttcggct
     3301 gccgctgatc gtgccgttaa tcccctcttc cgcaaggagc agcgtgcctt tcaccccatg
     3361 agccttcatg gctgcaagca ggggttgcct gaggctggca aagtccttca gtgtgacaaa
     3421 cttgtagagg gcggcaacaa caattttgtg gggcatgaac caagctttta ttaggccttt
     3481 ccatgttttt caattgcgca tcaggcaggg cgtcccgtaa gtaacggcaa ccccagagga
     3541 caaatgccag agttcattct aaacgccggt gtggagattc cttttattct cattctgctg
     3601 gcgcttctgg gcttccgccg gaaagtgaac ccccgggcga aactcctgat tagggctcca
     3661 caggaaaagg tctttgccct tgtcgacctc catcacggca aggtgcagac ctggagccgc
     3721 accacagtca cgtccgagct tcttgatgac gcccgccagg ttttccgcat gacttatgtg
     3781 accaccctct cgaccggcgc ccagcaaaaa tcccaggccc atttccgtgt ggccgaacgg
     3841 cgccctcccg cctacctaga actgcaccgc gacgggctgg agggcaaatc cctcaacaat
     3901 gagctgctga agatcgtcac ggaaactgag gcccaccccg aaggcacctg gctcacccag
     3961 aaatacaaat gggg
//
LOCUS       DAORVN010000098         3965 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_17885_length_3965_cov_3.025320, whole genome shotgun sequence.
ACCESSION   DAORVN010000098 DAORVN010000000
VERSION     DAORVN010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3965
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17885_length_3965_cov_3.025320"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1090
                     /locus_tag="PKV23_02890"
     CDS             <1..1090
                     /locus_tag="PKV23_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019964442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminopeptidase family protein P"
                     /protein_id="HQX84411.1"
                     /translation="DKAKLPEDVQAHLKKSVTIRKPGDLEPALKALGKSTVQIDGAWA
                     PARIKAALGKARIINATDLCVLPKAKKNKTEQEGARAAHRRDGVAMSRFLCWLESAAP
                     LGGLTEWSVAEKLQQFRKDTGMLLDLSFDSIAGAGPNAAIPHYHVNPDNCLPLRINEI
                     FLIDSGGQYQDGTTDITRTVIVGEPTDEMRDRFTRVLKGMIDVSCIRFPKGTCGSQID
                     VLARQSLWAAGLDYDHGTGHGVGSYLSVHEGPARINKSDRTPLEPGMILSNEPGFYKQ
                     NEYGIRIENLLLIHEAKAIDGGERPMLGFETLTLCPIDRRLIDTKLLTRDELHWLDTY
                     HARVLKEVGDHLSGDELTWLRKACAPFN"
     gene            complement(1083..1379)
                     /locus_tag="PKV23_02895"
     CDS             complement(1083..1379)
                     /locus_tag="PKV23_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011974933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HQX84412.1"
                     /translation="MTVTILAILGMALATYATRASGPYLMRGVVVKGRLKAALDALPP
                     AILMAVIAPTILTTGLAESIAAVLTAAVAFMRAPLVVIVLTGVISVVLLRMVLS"
     gene            complement(1376..2068)
                     /locus_tag="PKV23_02900"
     CDS             complement(1376..2068)
                     /locus_tag="PKV23_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007605765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HQX84413.1"
                     /translation="MTSTAREFRQGLLDVLPILVAAAPIGLLWGTLAASKGLVPFEVW
                     LMSATVFAGAAQFVAIEVWRDPVPWVLLTVTALIVNIRHVLMGASLSRHLGGIGRRWH
                     PFMMFLMADENWAFSERRALKTPLSLGYYFGLALPMWITWTTSSITGAIVGRSFGDPA
                     AYGFDFAFSAMFIGILAGFWKGSRTAAILGSSAVAAALSKLYVPGAWYIVIGGIVGVL
                     VAALLYREGERS"
     gene            complement(2065..2928)
                     /locus_tag="PKV23_02905"
     CDS             complement(2065..2928)
                     /locus_tag="PKV23_02905"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HQX84414.1"
                     /translation="MKLLAPDDTLDDLGENDETSFWRVNRHESLECLSATFRTHVFPP
                     HTHESYVIGAVVGGVNVYRISGLLLRAGPGELCFVHPGEVHDAVPEGDGYSYRMIYPK
                     PSLLQNIVEMATGRRANAPQFNGPVIRDEQLAARYLRAHEALEAGAEPLGVDEALTAV
                     LLDMIDRHAGGLNAVGVAGREPLAVRRAKDHLAAHVDEQVELENLARAAGLSPFHLIR
                     VFRKATGMTPHNWLIDRRVHMACNLLRAGLSATHVAAQCGFSDQSHLTRVFKSRLGIT
                     PGRFRLSKICA"
     gene            complement(2977..>3965)
                     /locus_tag="PKV23_02910"
     CDS             complement(2977..>3965)
                     /locus_tag="PKV23_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HQX84415.1"
                     /translation="EIAPKDIRIGDTVVVQRAGDVIPQIVAVILDKRPRGAKPYEFPK
                     VCPACGSHAEREERETGKTDVVRRCTGGLICPAQRVERLKHFASRNAFDIEGLGDKII
                     EEFYHDGLIQSPSDIFTLAARNERSLKKLKDREGWGEASVKKLFDAIDQRRSIALDRF
                     IYALGIRHVGETTARVLARAYGSAEHFRDQMIAASHHDEEAWKELNAIDGIGEVVAEA
                     ITQFFGEGHNIEVVNELLKHVTPAALAAVAASSAVSGKTVVFTGALELMTREEAKSMA
                     ERLGAKVAGSVSKKTDLLVAGPGAGSKLKEAEKHGVEVIDEAEWFKRVGEKK"
BASE COUNT          819 a         1328 c         1106 g          712 t
ORIGIN      
        1 cgacaaggca aaactgccgg aagatgtgca ggcccatctc aaaaaatccg tcaccatcag
       61 aaagcccggc gaccttgagc ccgccttgaa ggcgctggga aaatccaccg tgcagattga
      121 tggcgcatgg gcgcctgcga ggatcaaagc ggcccttggc aaggccagga tcatcaacgc
      181 caccgatctc tgcgttctgc ccaaggcgaa gaagaacaag acagagcagg agggagcccg
      241 cgccgcgcac cggcgtgatg gcgtcgccat gtcccgcttc ctttgctggc tcgaatctgc
      301 cgcgccgctt ggcgggctca ccgaatggtc cgtggccgaa aagctgcagc agttccgcaa
      361 ggacaccggc atgctgcttg acctctcttt cgacagcatt gcgggcgctg gccccaacgc
      421 cgccattccg cactatcacg tgaacccgga caattgcctg ccactcagga tcaatgaaat
      481 cttcctcatc gattccggcg gccaatacca ggacggcacc accgacatca cccgcaccgt
      541 catcgtcggc gagccaaccg acgaaatgcg cgatcgtttc acccgcgtgc tgaaaggcat
      601 gatcgatgtt tcatgcatcc gctttcccaa aggcacctgc ggctcgcaga tcgatgtgct
      661 tgcccgccaa agcctgtggg ccgcaggtct tgattatgac cacggcacgg gccacggcgt
      721 cggctcctat ctctctgtgc atgaaggccc ggcccgcatc aacaagtcgg accgcacgcc
      781 gcttgaaccc ggcatgatcc tttccaacga gcccggattc tataagcaga atgagtacgg
      841 catccgcatt gaaaatcttc tgctcattca cgaagccaag gccatcgacg gcggtgagcg
      901 ccccatgctg ggcttcgaga ccctgacgct ctgccccatt gatcggcggc ttatcgatac
      961 caaattgctg acccgtgacg agctgcattg gctcgacacc taccacgccc gcgttctcaa
     1021 ggaagtcggc gatcaccttt ccggcgatga actgacctgg ctgcggaaag cctgcgcgcc
     1081 ttttaactga gaaccatccg cagcagcacc acgctgatca caccagtcag gactatgact
     1141 acaagcggcg cccgcatgaa ggccaccgcc gccgtcagca ccgccgcaat ggattcggca
     1201 aggcctgttg tcaggatcgt tggcgcgatc accgccatca ggatagccgg cggcagcgcg
     1261 tcaagcgctg ccttcagccg ccccttcacc accacgccgc gcatgaggta cggcccgctg
     1321 gcgcgcgtcg cataggtcgc aagcgccatg cccaggatgg ccagaatcgt caccgtcatg
     1381 acctctcgcc ttcacgatag agaagtgctg caacaagcac gccaacaatg ccgccgatca
     1441 cgatatacca ggcgccgggc acatacagct tggacagggc cgccgcgacg gcgctggacc
     1501 ccagaatcgc agcggtgcgt gagcccttcc agaagccggc gagaatgccg atgaacatcg
     1561 ccgaaaatgc aaaatcaaat ccgtaagccg ccggatcgcc gaacgaccgg ccgacgattg
     1621 cgcccgtgat tgaagaggtt gtccaggtaa tccacatggg gagtgccagc ccgaaataat
     1681 agcccaggct gagcggtgtc ttgagcgccc ggcgctcgct gaaggcccag ttttcatcgg
     1741 ccatcaggaa catcatgaag ggatgccagc gccggccgat gccacccagg tggcgtgaca
     1801 gggatgcccc catcaacaca tggcggatat tcacaatcag cgccgtgact gtgagcaaga
     1861 cccagggcac tggatcgcgc catacttcaa tggctacgaa ttgcgccgcc ccggcaaaaa
     1921 ccgtggcgct catcagccag acttcaaaag gtacgagccc cttgctcgcc gccagggttc
     1981 cccacagcaa gccgatgggc gcggcggcaa ccagtatggg caggacatca agcagcccct
     2041 gccggaactc acgcgccgtt gacgtcatgc gcagatcttg ctcaggcgga acctgccggg
     2101 cgtgatcccc agccgcgact tgaacacgcg cgtcagatgg ctctggtcgg agaagccgca
     2161 ttgcgccgcc acatgggtgg cgctcaggcc ggcccgcagc aggttgcagg ccatatgcac
     2221 ccgccggtca atgagccagt tgtgcggcgt catgcccgtg gccttgcgaa acacccggat
     2281 caggtggaaa ggactgagac ccgccgcccg cgccagattt tcaagttcca cctgctcgtc
     2341 gacatgagcc gccaggtgat ccttggcccg gcgcaccgcg agaggctcgc ggccggcaac
     2401 tccgactgca ttcaggcccc cggcatgacg gtctatcatg tcaagcagca ccgcggtcaa
     2461 cgcctcgtca accccaagtg gctccgcccc ggcttcaagc gcctcatggg cgcgcaggta
     2521 acgtgcggcc agctgctcgt ccctgatgac cgggccattg aactgcggcg cattggcccg
     2581 ccggccggtc gccatttcca cgatgttctg cagcagcgac ggttttggat agatcatccg
     2641 gtagctgtag ccgtcgccct ccggcaccgc gtcatgaacc tcgccgggat ggacaaagca
     2701 gagctccccc ggccccgccc gcagcagcaa accgctgatc cggtaaacat tcacgccacc
     2761 taccaccgcg ccgatcacat agctttcatg agtatggggc gggaaaacat gggtacggaa
     2821 ggtggccgac aggcattcca gggactcatg ccggttcacc cgccagaatg acgtttcatc
     2881 attctcgccg aggtcgtcca gcgtatcgtc gggagcaaga agtttcatcg gtctaattta
     2941 gcaggccggg ccggaaaggc ttgtacagaa ttgcgcctac tttttctccc ccacccgctt
     3001 gaaccactcg gcttcatcaa tgacctcgac cccgtgcttt tcggcttcct tgagcttgga
     3061 gcccgccccc gggcccgcca cgagaagatc ggttttcttg gaaaccgaac ccgccacctt
     3121 ggcccccagc cgttcggcca tggattttgc ttcttcgcgg gtcatcaatt ccagtgcccc
     3181 ggtaaacacc acggtctttc cggaaaccgc tgacgaagct gcaacggctg caagcgccgc
     3241 cggcgtgaca tgcttcagca gttcgttgac cacctcgata ttgtggccct cgccaaaaaa
     3301 ttgcgtgatc gcttcggcaa cgacttcgcc gatgccatca atcgcgttga gctccttcca
     3361 cgcctcttca tcatgatgtg acgccgcaat catctgatcg cggaaatgct ccgccgagcc
     3421 ataggcccgc gccagaaccc gcgccgtggt ctcgcccaca tggcgaatgc ccagagcata
     3481 gatgaagcgg tcaagggcaa tgctgcgccg ctggtcgatg gcgtcgaaca gttttttgac
     3541 gctggcctca ccccacccct cgcggtcctt cagttttttc agtgagcgct cattgcgtgc
     3601 cgcaagggtg aagatgtcgg aaggcgattg aataaggccg tcatgataga attcctcgat
     3661 gatcttgtcg ccaagcccct cgatgtcgaa tgcatttcgc gaggcgaaat gcttgagccg
     3721 ctccacccgt tgcgccgggc aaatgagccc gccggtgcag cgccgcacca catcggtctt
     3781 gcccgtctca cgctcctcgc gctccgcatg gctgccgcag gccgggcaga cttttggaaa
     3841 ttcatagggc tttgcacccc gcggccgctt atccaggata accgcaacaa tctgcggaat
     3901 gacgtcgccc gcccgttgaa ccaccaccgt atcaccgatc cgaatatctt tcggggcgat
     3961 ttcat
//
LOCUS       DAORVN010000099         3957 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_17948_length_3957_cov_3.707586, whole genome shotgun sequence.
ACCESSION   DAORVN010000099 DAORVN010000000
VERSION     DAORVN010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3957)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3957)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3957
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17948_length_3957_cov_3.707586"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1099
                     /locus_tag="PKV23_02915"
     CDS             <1..1099
                     /locus_tag="PKV23_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HQX84416.1"
                     /translation="SYEELNGIANKLVEKLEDNPGLADLDTDLRLNKPEVRIEIDRAR
                     AADLGLDVTVIGRTLETLLGGRDVSTFEIGSEQYDVTVALPVEDRTSTETLSRIFVRS
                     ASGEMVQLSNVVTAREAVAPRDLKRFNQLRAITIEANLSPGFTLGEAIAAFNAAAQEV
                     LPDNVVTDLSGQARELRDSSSNLALVFVLALAFIYLVLSAQFESFRDPVMIMLSVPLS
                     MTGALAALYFTGGTLNVYSQIGLVTLIGLITKHGILIVDFSNRMQEEGRTRLDAIVEG
                     ATLRLRPILMTTSAMVLGAVPLVLASGAGAESRVQIGWVIVGGMTLGTLLTLFVVPCA
                     YAIMGRQHVGPALEEAKPELHFAKPLPKAAE"
     gene            complement(1118..1411)
                     /locus_tag="PKV23_02920"
     CDS             complement(1118..1411)
                     /locus_tag="PKV23_02920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX84417.1"
                     /translation="MNILLSSGMLNSILDHIEKARGKREILDVYKAAEMIRLEHISDN
                     VALEDIIEKIVLTAGSTLPMEFRRARSQPRQTGDAAIDSPVEFLLPDQALAAV"
     gene            complement(1568..1792)
                     /locus_tag="PKV23_02925"
     CDS             complement(1568..1792)
                     /locus_tag="PKV23_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013894480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX84418.1"
                     /translation="MSRERLVRLQIMLDNDELEAIETWRFQTRMPSRAAAVRELMRRG
                     LAAEGFKVAQVGGQSENYGVISSPVPDGEA"
     gene            2035..2535
                     /locus_tag="PKV23_02930"
     CDS             2035..2535
                     /locus_tag="PKV23_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007008315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HQX84419.1"
                     /translation="MSTFDLFFIRTALPETEIVVRLIVSLLLGGVIGFDREVKNRPAG
                     LRTHMLVALAASIFSITTLEIIAGLEKTGHTGDPLRAIEAVTAGVAFLAAGTIIQSRV
                     QISGLTTGASMWLAGAVGVACGFGFFSIAVLATGLALIVLVALQRLSEKINPTAAPAA
                     ETKVKR"
     gene            complement(2525..3004)
                     /locus_tag="PKV23_02935"
     CDS             complement(2525..3004)
                     /locus_tag="PKV23_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009734288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HQX84420.1"
                     /translation="MSLESVRAFLAENAPDIAIIELENSSATVALAAAGHGVTRGQIA
                     KTLSLRVGDRNLVVVTSGDARLDNKKARAAFGGKPRMLSPEEVLAVTGHPVGGVSPFG
                     LASALPVYCDVSLKAFDEVVPAAGSTNSALRINPIRMAELLGAEWVDVCQTDTPATV"
     gene            complement(3182..3316)
                     /locus_tag="PKV23_02940"
     CDS             complement(3182..3316)
                     /locus_tag="PKV23_02940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX84421.1"
                     /translation="MAQIRFNDARQWIDGRAPQFPLIMAKFFGRTIDVNQDPLLAHPA
                     "
     gene            3315..3869
                     /locus_tag="PKV23_02945"
     CDS             3315..3869
                     /locus_tag="PKV23_02945"
                     /inference="COORDINATES: protein motif:HMM:NF013460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HQX84422.1"
                     /translation="MPVTARTRYDTVAMSLHWLIAALLIFMLFFGEEIMETEVGGGAF
                     GPSLHVSIGSAILVLSVLRLVWRVINPPPAYSASMAPWERLAATATHVLFYVLLIGIP
                     LTGWLATPKFLRDEDALAGLTLFGAFPLPAAPSLGLPMKGIHEIGSNLGIVLLALHVL
                     AALKHHFLNRDDVLRRMLPFGRAR"
BASE COUNT          751 a         1187 c         1170 g          849 t
ORIGIN      
        1 ctcctatgag gagctcaacg gtattgccaa caagctggtg gaaaagctcg aggacaaccc
       61 gggccttgcc gacctcgata cagacctgcg gctcaacaag ccagaggtgc ggattgaaat
      121 tgaccgggcg cgagccgccg acctcggcct ggacgtgacg gtgatcggac gcacgctgga
      181 aacgctgctc ggtgggcgcg acgtcagtac ctttgaaatc ggcagcgaac aatatgacgt
      241 gacggtggca cttccggtgg aggaccgcac ttcgaccgaa acgctttcgc gcattttcgt
      301 gcgcagcgct tcgggcgaaa tggtccagct ttccaacgtg gtgacggcgc gcgaggccgt
      361 ggcaccgcgc gacctcaagc gtttcaacca gcttcgcgcc atcaccatag aggccaatct
      421 ttcgccgggc ttcacgctgg gcgaggcgat tgccgccttc aatgcggcag cccaggaagt
      481 gctgcctgac aatgtggtga cggacctttc aggccaggcg cgcgagctgc gcgactcaag
      541 ctccaacctg gcgctggttt tcgttttggc gctggcgttc atctatctcg tgctttcagc
      601 gcagtttgaa agcttcaggg atcccgtgat gatcatgctg agcgtgccgc tgtccatgac
      661 gggggcgctg gcggcgcttt atttcacggg cggcaccttg aacgtgtatt cgcagatcgg
      721 attggtgaca ctcatcgggc ttattaccaa gcacggcatc ctcatcgtgg atttttccaa
      781 ccggatgcag gaggaaggcc gcacgcggct tgacgccatc gtcgaagggg cgacgctgcg
      841 gctgcggccc attctcatga ccacttccgc catggttctg ggcgcggtgc ctcttgtgct
      901 ggcctccggc gccggtgcgg aaagccgggt gcagatcggc tgggtgattg ttggcggcat
      961 gacgcttggc acactgctca cgctgttcgt ggtgccctgc gcctacgcca tcatgggccg
     1021 gcagcatgtt ggcccggcgc ttgaagaggc gaagcctgaa ctgcatttcg caaagccgct
     1081 gcccaaggct gcggagtagt tcgccgcaaa cctagggtca gacagcagcc agggcctggt
     1141 cgggcaggag aaattctact ggcgaatcaa tggcagcgtc ccccgtctgc cgaggttgac
     1201 tcctggctct cctgaattcc atgggaagag tggaacctgc agtcaggaca attttctcga
     1261 ttatatcctc aagcgcgaca ttgtcgctga tatgctcgag acggatcatc tcggcggcct
     1321 tgtagacatc gagtatctca cgcttaccac gcgccttctc gatgtgatcg agtatgctgt
     1381 tcaacatgcc ggaactgagc aagatgttca tgggcgatct cctgttttga gatcactctg
     1441 cgcttcgtgc tgatttaatc aatccgccgt ttgtgccaga agctgcatgc gcgccaagtg
     1501 ctgcatgtgc gcgttccgtg catcgtccgg caagtccgca caatgagtag tcttagcaaa
     1561 tcaggactca agcttcgcca tcgggaacag gggaactgat cacgccatag ttttccgact
     1621 ggccgccgac ttgcgcgacc ttgaagccct ccgccgccag gccgcggcgc atcaattcgc
     1681 ggaccgccgc ggcacggctc ggcatgcggg tctggaagcg ccaggtttca atggcttcga
     1741 gttcatcgtt gtccaacatg atctgcagcc gcacgagccg ttctctactc atatcaatct
     1801 ccgtaaatcg gcaaacagaa acgatagact ggcaaccctg accaaaaatc agctcttttc
     1861 gacagccctg caagatgaga caagtcagca aatggcgtca agttagtgtc cggtagcgaa
     1921 cgcaaatcct gcccctccgg ttccaacacc gagtgaaaaa atcaattttt cagccttatg
     1981 gaactttttt ctttgtgatc cgttgcggtt tctcatactg aggaactgcg ggacatgagt
     2041 acattcgacc tctttttcat ccggaccgct ctgccggaga cggagatcgt tgtcaggctg
     2101 atcgtatccc tgctcctcgg cggtgtcata ggtttcgacc gtgaagtcaa gaaccgccct
     2161 gccgggctgc gcacccacat gctggttgcc cttgccgcgt caatcttttc aatcaccacg
     2221 ctggagatca tcgccgggct ggaaaaaacc gggcatacgg gcgaccccct ccgggccatt
     2281 gaagcggtga ccgccggcgt ggcgtttctt gcagcgggca ccatcatcca gtcgcgggtg
     2341 cagatttccg gcctgacaac gggggccagc atgtggctgg ccggcgcggt gggcgttgcc
     2401 tgcggatttg gctttttctc cattgcggtg ctggccacgg gccttgccct catcgttctg
     2461 gtggcactgc agcgcctttc ggaaaaaata aatccaaccg cagccccggc ggccgagacc
     2521 aaggtcaaac ggtagctggc gtgtccgtct ggcagacatc gacccactct gcgccaagca
     2581 gttcggccat gcggatggga ttgatgcgga gcgcgctgtt tgtcgaaccc gccgcgggca
     2641 ccacctcatc aaaggctttc agcgagacgt cacaatagac gggcaaggcc gaggccagcc
     2701 caaagggact gaccccgccc accgggtggc cggtcaccgc cagtacctct tcggggctga
     2761 gcatgcgggg cttgccgccg aaggcagccc tggccttttt gttgtcgagg cgtgcgtcgc
     2821 cgctggtcac aaccacgagg ttacggtcgc ctacgcgcag tgaaagggtc ttggcgatct
     2881 ggccgcgggt aacgccatgg cctgccgccg ccagcgccac ggtggcgctg ctgttttcca
     2941 gttcaatgat ggcaatgtcg ggggcgtttt cagccaggaa ggcccggaca gactctaagc
     3001 tcatgggatg cagcctttct tcagcacccg agaattatcc gccccgccgc accattcaag
     3061 aggaaaaggc gtaaggtaca gggaaacaac ctggtacccc aattatgcct tcttgcgctg
     3121 catcttcggt tctccattta cgccaggtaa caattcgtaa tcaaggccgc ctgacctctc
     3181 gtcaagccgg atgagccagc agcgggtctt gattcacatc aatggtgcga ccaaaaaatt
     3241 tcgccatgat caacggaaat tgcggggcgc gcccgtctat ccattgccga gcatcgttga
     3301 aacggatttg agccatgcct gttaccgcaa gaaccagata tgacactgtt gccatgagcc
     3361 tgcactggct gatcgccgca ctgctgatct tcatgctctt ctttggcgag gagatcatgg
     3421 aaacggaagt cggcgggggt gcgttcggac cgtctctcca tgtttcgatc ggcagtgcca
     3481 ttctcgtgct cagcgtattg cgcctcgtct ggcgcgtgat caatccgccg cctgcctatt
     3541 ccgccagcat ggccccctgg gagcggctgg cagctacagc aacgcatgtg ctattttatg
     3601 tcctgctgat tggcattccg ctcacgggct ggcttgccac gccgaagttc ctcagggatg
     3661 aagacgcact cgcaggcctc acgctcttcg gcgcatttcc gctgcctgcg gcgccaagcc
     3721 ttggcctccc catgaagggc attcacgaga tcggttcgaa cctcggcatc gtgctgctgg
     3781 cgctgcatgt gcttgccgcg ctcaagcatc acttcctcaa ccgggatgac gtgctgcgcc
     3841 gcatgctgcc ctttgggcga gcccgctagc ttccaggcaa ttttttgggc tgcggcgcgg
     3901 ggcagttgtg gcgcggcaga ggaatctgct cgtaagggtt atcggcgcgg gccaagg
//
LOCUS       DAORVN010000100         3957 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_17953_length_3957_cov_3.384931, whole genome shotgun sequence.
ACCESSION   DAORVN010000100 DAORVN010000000
VERSION     DAORVN010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3957)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3957)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3957
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17953_length_3957_cov_3.384931"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="PKV23_02950"
     CDS             <1..291
                     /locus_tag="PKV23_02950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX84423.1"
                     /translation="DAVRVWVISLKPGERLPYHCHVLNYFWTATSEGATRSHYSDGTT
                     AESSVRTGDTRHYRFAAGERMIHDLENIGATPVSYVTVELKIGSANAPLALA"
     gene            complement(288..1052)
                     /locus_tag="PKV23_02955"
     CDS             complement(288..1052)
                     /locus_tag="PKV23_02955"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015916435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HQX84424.1"
                     /translation="MSCKLSVNVNAIAFLRNRRNLPWPSLEGLGRIALQAGAAGLTIH
                     PRPDERHIRQADVPVLRGLIRKEFPSREYNIEGYPDERFLKLVEANRPDQVTLVPDDP
                     AQGTSDHGWDIARNHGLLTDVIARLHAGKMRVSLFVDADPKLAEQAKAVGADRIEIYT
                     GPYGGAFDPASRVREFDKIVVAGKVAEFAGLGLNAGHDLTRENLPALVAALPNLAEVS
                     IGHAIIADALTFGMSETVRLFREAIGDVDVAARRTA"
     gene            complement(1121..1411)
                     /locus_tag="PKV23_02960"
     CDS             complement(1121..1411)
                     /locus_tag="PKV23_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013503819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HQX84425.1"
                     /translation="MQILSPAHPGEVIKEDVIGAYGLSVTDAARVLGVGRPALSAVLN
                     GRASLSPDMALRCEKAFGISMDLLLKMQLQFDIAQVRKAAKNMKVQKFKPAA"
     gene            complement(1420..1674)
                     /locus_tag="PKV23_02965"
     CDS             complement(1420..1674)
                     /locus_tag="PKV23_02965"
                     /inference="COORDINATES: protein motif:HMM:NF016875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HQX84426.1"
                     /translation="MQRFAETGDPRGLPADCIPRLKRLVSALNAAESLEELGSVPGWR
                     LHVLKGDRSGYWSLSVSGNWRLIFKWSNGRAEELDFVDYH"
     gene            complement(1750..2310)
                     /gene="ilvN"
                     /locus_tag="PKV23_02970"
     CDS             complement(1750..2310)
                     /gene="ilvN"
                     /locus_tag="PKV23_02970"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HQX84427.1"
                     /translation="MNAHQQPTGSAYFLDTSKKTVETHTLAVVADNEPGVLARIIGLF
                     AGRGYNIDSLTVSETIHDKHVSRFTIVTTGTPSVLTQIRHQLERMVPVHSVADLTVQG
                     NSIARELAMVKVAGGGEKRMEALRLADAFRARVIDASTDSFIFEITGKPDKVDQFISL
                     MVPIGLVEVSRTGVAGISRGPEGMKV"
     gene            complement(2321..>3957)
                     /locus_tag="PKV23_02975"
     CDS             complement(2321..>3957)
                     /locus_tag="PKV23_02975"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HQX84428.1"
                     /translation="RHILVRHEQGAVHMAKGYARSTGKPGVVLVTSGPGATNAVTGLV
                     DALMDSIPLVVLSGQVPTFMIGTDAFQECDTVGITRPCTKHNWLVKDVNDLARILHEA
                     FYVATHGRPGPVVVDIPKDVQFAKGKYVGPKNIEHKTYRPQVKPNQDAIRAAVELISK
                     AKRPLLYTGGGVINSGVIASKLLREFVKMTNIPITSTLMGLGAYPASGKNWLGMLGMH
                     GTYEANMAMHDCDVMINVGARFDDRITGRLDAFSPGSKKIHIDIDPSSINKTVQVDIP
                     VVGDVAHALEDIIRVWKAKSHELDKKALAAWWKEIDVWRAKDSLKYRANKDVIMPQYA
                     IQRLYELTRDKEVYITTEVGQHQMWAAQFFKFEDPHHWMTSGGLGTMGYGLPAAVGVQ
                     MAHPKALVVDIAGDASVQMTMQEMSTAVQYRLPIKIFILNNQYMGMVRQWQQLLHGNR
                     LSESYSESLPDFVKLAEAFGCVGLRAEKPAELDDRIREMIKTPHPVLFDCRVASLANC
                     FPMIPSGKAHNEMLLGEDVSDEEVGTAIDAKGKELV"
BASE COUNT          780 a         1239 c         1134 g          804 t
ORIGIN      
        1 gatgcggtgc gggtgtgggt gatatcactg aagcccggcg agcgcctgcc ctatcactgc
       61 catgtgctga attatttctg gaccgccacc tccgagggcg ccacccgctc gcattacagc
      121 gatggcacaa cagccgaaag cagcgtcagg accggcgata ccaggcatta caggtttgcg
      181 gccggcgaaa gaatgatcca cgaccttgag aacataggcg cgacgcccgt ttcctacgtc
      241 accgtcgaac tgaaaatcgg cagcgccaac gcgccgctgg cgctggccta ggcggttctg
      301 cgtgccgcca cgtcaacatc gccaatggcc tcccggaaca ggcgcaccgt ctccgacatg
      361 ccgaaagtga gcgcgtcggc aataatcgcg tgaccgatgg aaacttccgc gaggttcggc
      421 agtgccgcca cgagcgccgg caggttttcc cgcgtcaggt catggcccgc gttaaggcca
      481 aggccggcaa attccgccac cttgccggcc accacgatct tgtcgaattc gcgcacccgg
      541 gatgcggggt caaacgcccc gccatagggc cccgtataaa tctcgatgcg gtcggccccc
      601 acggccttgg cctgctcggc cagtttcggg tcggcatcaa caaacagaga gacacgcatc
      661 tttccggcat gaagccgcgc gatcacatcc gtgagaagcc cgtggttcct ggcaatgtcc
      721 cagccatggt cggaggtgcc ctgggccgga tcatcgggca ccagcgttac ctgatccggc
      781 ctgttggcct ccaccagctt gagaaagcgc tcgtcgggat agccttcgat attgtattcg
      841 cggctgggaa attccttccg gatcaggccg cgcagcaccg gcacatccgc ctgccggata
      901 tggcgttcat cgggccgagg atgaatagtg aggccagccg cccccgcctg cagcgcaatg
      961 cgccccagcc cctcaaggct cggccagggc aggttccgcc ggttacgcag aaacgcaatg
     1021 gcattcacgt tgacagacag cttacagctc atggcgcagg aatgtagacg cgaaatggaa
     1081 tgccggaaag tccggtttta ataaattatg tcagaccact tcaggccgct ggcttgaatt
     1141 tctgcacttt catgttcttc gccgccttgc ggacttgcgc gatatcaaac tgcagttgca
     1201 ttttgagaag aaggtccatt gaaattccaa aggctttctc acagcggagt gccatgtcag
     1261 gtgaaaggct ggcacgccca ttcaaaaccg cgctcaaggc cggacgcccg acacccagta
     1321 cccgcgctgc atctgtgacc gacaggccat acgccccaat cacgtcttcc tttattactt
     1381 cgccgggatg agccgggctg agaatctgca tgatgcacct caatgatagt ccacaaaatc
     1441 caactcttcg gccctgccat ttgaccactt aaaaatcagc cgccaattgc cacttacgga
     1501 aaggctccaa tatccgctgc gatcgccctt gagaacatgc aggcgccatc cgggcaccga
     1561 acccagttct tccaggcttt cggcggcatt caaagctgaa acgagacgtt tcaaccttgg
     1621 gatacaatct gctggcagcc cgcgcggatc acccgtctct gcaaaccgct gcaaggcctt
     1681 atgcctgatt gaaccaatca cccgtagagt gtagcctgtt gcgcgacaga agtcaactca
     1741 gcgctctctt caaaccttca tgccttccgg cccgcgcgag atgccggcaa cgccggtgcg
     1801 cgagacttcg accaggccga tgggcaccat cagcgagatg aactggtcaa ccttgtcggg
     1861 cttgccggtg atttcaaaaa taaagctgtc ggtcgaagca tcaatgaccc gggcccggaa
     1921 cgcgtcagcc aggcgaagtg cttccatgcg cttctcgccg ccgccggcaa ccttcaccat
     1981 ggcaagctcg cgcgcgatgg aattgccctg caccgtgagg tcggccaccg aatgcactgg
     2041 caccatgcgc tcaagctggt gcctgatctg ggtgagcacc gatggcgtgc ccgtggtgac
     2101 aatggtgaaa cgcgagacat gcttgtcgtg gatcgtttcc gaaaccgtga gcgagtcaat
     2161 gttgtagccg cggcccgcga acaggccgat gatccgggca aggacgcccg gctcgttgtc
     2221 ggccaccacg gccagcgtgt gggtttccac tgtcttcttc gaggtgtcga ggaaataggc
     2281 cgagcccgtg ggttgttgat gtgcgttcat gtctctctcc tcaaaccagc tctttgccct
     2341 tggcgtcgat ggccgtgccg acttcctcgt cggacacatc ttcgcccagc agcatttcat
     2401 tatgcgcctt gcccgaaggg atcatcggga aacagttggc aaggcttgcc acgcggcaat
     2461 cgaaaagcac gggatgcggc gtcttgatca tctccctgat cctgtcgtca agctcggctg
     2521 gcttttccgc ccgcaggcct acgcagccaa aggcttccgc cagcttcacg aaatccggca
     2581 gcgattccga atagctttcg ctgagccggt tgccatggag cagttgctgc cactgccgca
     2641 ccatgcccat gtactggttg ttcagaatga agatcttgat cggcaggcgg tactgcactg
     2701 ccgtggacat ttcctgcatg gtcatctgca ccgaggcatc gcccgcgata tcgacaacca
     2761 gcgccttggg atgcgccatc tgcacgccca ctgcggccgg caggccatag cccatggtgc
     2821 cgaggccgcc cgacgtcatc cagtggtgcg ggtcctcgaa cttgaagaac tgcgccgccc
     2881 acatctggtg ctggccaact tccgtggtga tgtagacttc cttgtcgcgc gtgagttcat
     2941 acagccgctg gatggcgtat tgcggcatga tcacgtcctt gttggccctg tatttcaggc
     3001 tgtccttggc acgccaaacg tcaatttcct tccaccaggc cgccagcgct ttcttgtcga
     3061 gctcatggga cttggccttc cacacgcgga tgatgtcttc cagcgcgtgg gccacgtcgc
     3121 ccaccaccgg aatgtcaacc tgcacggtct tgttgatcga cgagggatcg atgtcgatgt
     3181 ggattttctt cgagcccggt gaaaaggcat cgagtctgcc cgtgatccgg tcgtcaaagc
     3241 gtgctcccac attgatcatc acgtcgcaat catgcatcgc catgttggct tcgtaggtac
     3301 cgtgcatgcc cagcatgccg agccagttct ttccgctcgc cggataggcg ccaaggccca
     3361 tcagcgtcga ggtgatcggg atgttcgtca tcttcacgaa ttcgcgcaac agcttcgagg
     3421 cgatcacgcc ggaattgatc acccccccgc cagtgtaaag cagcgggcgc ttggccttgg
     3481 agatcaattc aacggcggcg cggatggcgt cctggttcgg cttcacctgc gggcgatagg
     3541 tcttgtgctc gatattcttg gggccgacat acttgccttt ggcaaactgc acatccttcg
     3601 gaatatcaac cacgaccggg ccgggccgcc cgtgggtggc cacatagaag gcttcgtgca
     3661 ggatgcgggc gagatcattt acgtccttca ccagccagtt gtgcttggtg caggggcggg
     3721 tgatgccgac cgtgtcgcat tcctggaagg cgtcggtgcc gatcatgaac gtgggcacct
     3781 gacccgacag cacgaccagc gggatggaat ccatcagcgc atcgacaagg ccggtcaccg
     3841 cattggtggc gcccggaccc gatgtcacca gcacgacgcc cggctttccg gtcgagcggg
     3901 catagccttt ggccatgtgg actgcgccct gttcgtgacg cacgagaatg tggcgaa
//
LOCUS       DAORVN010000101         3957 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_17954_length_3957_cov_3.123783, whole genome shotgun sequence.
ACCESSION   DAORVN010000101 DAORVN010000000
VERSION     DAORVN010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3957)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3957)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3957
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17954_length_3957_cov_3.123783"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            71..850
                     /locus_tag="PKV23_02980"
     CDS             71..850
                     /locus_tag="PKV23_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018900969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HQX84429.1"
                     /translation="MANAIKKILLIGETWMSSATHYKGFDQFGSVTFHSGADPLVKAL
                     ADTAFELTHMPAHAAIEKLPFDLAGLQAWDVILLSDIGANSILLHPDVWLRGKTVPNR
                     LKLLRDWTAAGGGLAMIGGYFSFQGIDGRARWHRTPVEEVLPVECLPHDDRLEIPEGF
                     SAVVHGSASHAILKGITGEWPMLLGANEVRLKKIEGVEVLASLPAEQGGHPLLVTGKF
                     GKGRTLAWTSDISPHWLPASFYEWPGYTTLWRNIVGWLANS"
     gene            complement(870..1397)
                     /locus_tag="PKV23_02985"
     CDS             complement(870..1397)
                     /locus_tag="PKV23_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015824678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HQX84430.1"
                     /translation="MLNLMLLRHAKSSWAETGQADLDRPLNERGKRAAAAMGSYLAAN
                     SLVPQRVLCSPARRTKETWDLVAAELPGTPEVLVAPEIYDFSGGKALMECLRHKAGAA
                     NSVLLVGHNPSIAGLAQTLVGAGNGKLRQRLDEKYPTAALAVISFDLANWESLSAGAG
                     TLQRFVTPRDIIDDD"
     gene            complement(1403..2176)
                     /locus_tag="PKV23_02990"
     CDS             complement(1403..2176)
                     /locus_tag="PKV23_02990"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018700381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQX84431.1"
                     /translation="MPRFLNLHLGATFAFLYIPIAVLIVLSFNKAGLPTAWSGFSVEW
                     YGRLAANPKILASAWNSVIVATISTLIATVLGTLLALGVERAKPSPARDALLFAPMIV
                     PDIVLAIALLSFFTLIKFTLGLHSIILAHVVFNIAFVCAVVRARLKGFDWSLTEVSRD
                     LGAGHLVTFRKITLPLILPSVIAAALLAFTLSIDEFIIAYFTAGAGQASTTLPMQIYS
                     MIRFGVTPEINAMASIVMAVSFVLVLLAQRLNRGRMPGA"
     gene            complement(2169..3020)
                     /locus_tag="PKV23_02995"
     CDS             complement(2169..3020)
                     /locus_tag="PKV23_02995"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013892339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQX84432.1"
                     /translation="MPPRLISWLLMAPGLLWLTALMIVPCLLIFILAFFERGTYGGID
                     WSAATLENFARAFDHLYFSIFLASARIALIATVIALLIGYPAAYAIMKAPERWQTSLL
                     FLAILPFWTNYLIRTYAWIVLLNPVGVINNVLIYLGVISQPLPLLYNEFAVILGLVYN
                     YTPFVILAIYSALQRLDPSYAEASRDLGASAVTTFVRITLPLTAAGVAAGAVFVFVLS
                     IGNFVTPDLLGGGKLQMIGNLIYDQFLTARDWPFGATLSGLLIALMMIALFMQAVATG
                     RARGDHA"
     gene            complement(3199..>3957)
                     /locus_tag="PKV23_03000"
     CDS             complement(3199..>3957)
                     /locus_tag="PKV23_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013528946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HQX84433.1"
                     /translation="IDHGKIPNMANLYAEAGQLKHDPGNTFSVPYAWGTTGLCYRSDL
                     VKAEPTSWNDLLKPTDDLKGKITMLATDRWLMAAAFLAGGLSVNTDDQASLDKVRDQL
                     VATKKDLLAYDDTTFYSKLVSGEATLVHAWDGWCNYGIAENAKIKYVVPKEGSDLWVD
                     TMVITKASKNPDAAAKFIDFVLKADNGKWVVENIMYKTPNKAGMEAIDQSMLTTYPNM
                     AITPADLLKYEQLRDLGDGMKAFSKTVSEIMAAK"
BASE COUNT          847 a         1089 c         1289 g          732 t
ORIGIN      
        1 aaccgccagt atccaaagcc agtgtaatcg gtttaaatgc cataaccaac acaaaccggg
       61 agcatcagcc ttggcaaatg cgatcaagaa aattctgctc atcggcgaaa cctggatgag
      121 ttctgccacc cactacaagg gttttgacca gttcggcagc gtcaccttcc attccggcgc
      181 tgacccactg gtcaaggcgc tggccgacac ggctttcgaa ctgacccaca tgcccgctca
      241 tgcggccatt gaaaaactcc ccttcgatct tgctggcctg caagcctggg acgtgatcct
      301 gctatcggac atcggggcga attcaatact gcttcatccc gatgtctggc tgcgcggaaa
      361 aaccgtaccg aaccgcctca agctgttgcg ggattggacc gccgcgggtg gcggcttggc
      421 gatgatcggc ggctatttca gttttcaggg cattgatggc cgggcccgct ggcaccggac
      481 gccggttgaa gaggtcctgc cggtggaatg cctgccccat gacgacaggc ttgaaattcc
      541 ggaagggttc agcgccgttg tccatgggtc ggcctcccac gcaatcctca agggcatcac
      601 cggggaatgg ccgatgctgc taggtgctaa tgaagtcagg cttaagaaga ttgagggcgt
      661 cgaagtgctg gcaagccttc cggcggaaca gggcgggcac ccgctgctgg tcactgggaa
      721 attcggcaag ggccgcacgc tggcctggac ttcggacatc agcccgcact ggctgccggc
      781 ctcattttat gaatggccgg gttacaccac gctttggcgc aacatcgtgg gctggctggc
      841 aaacagctga gtgcgcccta gaagaacttt cagtcgtcgt cgatgatatc cctgggcgtg
      901 acaaagcgct ggagcgtacc tgcgccagcg gacagggatt cccaattggc gagatcaaag
      961 ctgataacgg cgagggccgc cgtagggtat ttctcgtcca gccgttgccg gagtttgccg
     1021 ttgccggctc caacgagggt ttgggccaat ccggcaatcg acgggttgtg gccaacaagc
     1081 agaaccgaat tcgcagctcc ggccttatga cgcaggcatt ccatgagcgc cttgccgcca
     1141 ctaaaatcgt aaatttctgg cgcgacgaga acttcagggg tgcccggcag ttctgctgca
     1201 accaagtccc aggtttcctt tgtccgccgc gccggtgaac agagcacacg ttgaggcacg
     1261 aggctattgg ctgccaagta gctgcccatc gccgcggcgg cgcgtttgcc gcgctcgttg
     1321 agtggccggt caaggtcagc ctgtccagtt tcagcccagc tcgacttggc atggcgcaga
     1381 agcatcaggt tcagcatcgt tttcaagctc ccggcatgcg gccacggttg aggcgctggg
     1441 cgagaagcac caggacgaaa cttacagcca tgacgatgct ggccatggca ttgatctccg
     1501 gggtcacgcc gaagcggatc atggaataga tctgcatggg aagggtcgtt gaagcctggc
     1561 cggcgccggc ggtgaagtag gcgatgatga attcatcgat cgacagcgtg aaagccagga
     1621 gtgctgcggc aatgaccgag ggcaggatga gcggcagggt gattttccgg aaggtgacga
     1681 gatggccggc gccgagatcg cgcgacactt cggtgagtga ccagtcgaag cccttcagcc
     1741 tggcgcggac cacggcgcag acgaaggcga tgttgaacac cacatgggcc aggatgatgg
     1801 aatgcaggcc gagggtgaac ttgatgagcg tgaagaaaga gagaagcgca atggcaagca
     1861 cgatgtccgg cacgatcatg ggcgcaaaca gcagggcgtc gcgggccggc gatggctttg
     1921 cccgctccac gccgagggcc agcagggttc cgagcacggt tgcgataagg gttgagattg
     1981 tggcgacgat taccgaattc caggcggagg cgaggatctt gggattggcc gccagcctgc
     2041 cataccactc gaccgagaat ccggaccatg cggtgggcag gcccgccttg ttgaaggaca
     2101 gcacgatgag caccgcaatc ggaatgtaaa gaaaggcgaa ggtggcgccg agatggaggt
     2161 tgagaaatct aggcatggtc gccccgggcc cggccggttg ccacggcctg catgaagagg
     2221 gcgatcatca tcagggcgat cagcaagccg gaaagcgttg cgccgaaagg ccagtcacgc
     2281 gccgtgagga actggtcata gatcaggttg ccgatcatct gcagcttgcc gccgcccagc
     2341 aggtcgggcg tcacgaaatt tccaatggac agcacgaaga cgaagaccgc gcctgccgcc
     2401 acgcccgccg cggtgagcgg cagtgtgatc ctgacaaatg tcgtgacggc agaagcaccg
     2461 agatcgcgcg aggcttcggc ataggaggga tccaggcgct gcagggccga atagatggcg
     2521 aggatgacga agggggtgta gttgtagaca aggccgagaa tgacggcgaa ttcattgtag
     2581 agcaggggca ggggctgtga aatcacaccc agataaataa gcacgttgtt gatcacgccc
     2641 accgggttga gcagaacgat ccaggcatag gtgcggatca ggtagttggt ccagaagggc
     2701 aggatggcaa ggaacagcag cgaggtttgc cagcgctcgg gggccttcat gatggcatag
     2761 gccgccggat agccgatgag gagcgcaatc acagtcgcaa tcagcgcgat gcgggcactg
     2821 gcgaggaaga tcgagaaata gagatggtcg aaagcgcggg caaaattttc gagcgtggcg
     2881 gcggaccagt caatgccgcc ataggtgccg cgctcgaaaa aagcgagaat gaaaatcaga
     2941 aggcatggca cgatcatcag cgccgtcagc caaagcaggc ccggcgccat gagcagccat
     3001 gaaatgagac gtggcggcat cagttaacct tcacgcttca tcggaatgac ggaagatagg
     3061 agcaagcggc gtcgattcta aattcgtcat tcccgcgaac gcgggaatcc atcgtggacg
     3121 cagcaaaggt ggatccccgc ttgcgcgggg atgacgaaac tggggaagcg tcggccacgt
     3181 cccgaccctc cccaagactc acttcgccgc catgatttcc gagacggttt tcgaaaacgc
     3241 cttcatgccg tcgcccaggt cgcggagctg ttcgtatttg agcaggtcag ccggcgtgat
     3301 cgccatgttg ggataggtgg tcagcatgct ctggtcgatg gcttccatgc cggccttgtt
     3361 gggcgtcttg tacatgatgt tctcgaccac ccatttgccg ttgtcggcct tgagcacgaa
     3421 gtcgatgaac ttcgccgcgg catcgggatt cttcgaggcc ttggtgatca ccatggtatc
     3481 gacccagagg tctgagcctt ccttgggcac cacgtacttg atcttggcgt tttcggcgat
     3541 gccgtagttg caccagccgt cccaggcatg aaccagggtc gcctcgccgg acaccagctt
     3601 ggaatagaag gtggtgtcgt cataggccag caggtccttc ttggtggcca cgagctggtc
     3661 gcgcaccttg tcgaggctgg cctggtcatc ggtgttcacc gacaggccgc cggccaggaa
     3721 ggccgccgcc atcagccagc ggtcggtggc cagcatggtg atcttgccct tgaggtcatc
     3781 ggtgggcttg agcaggtcgt tccagctggt gggctcggcc ttcaccaggt cggagcggta
     3841 gcagaggccg gtggtgcccc aagcataggg caccgagaag gtgttgcccg gatcatgctt
     3901 gagctggccc gcttctgcat agagattggc catgttggga atcttcccat ggtcgat
//
LOCUS       DAORVN010000102         3956 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_17960_length_3956_cov_3.186875, whole genome shotgun sequence.
ACCESSION   DAORVN010000102 DAORVN010000000
VERSION     DAORVN010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3956)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3956)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3956
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17960_length_3956_cov_3.186875"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..688
                     /locus_tag="PKV23_03005"
     CDS             <1..688
                     /locus_tag="PKV23_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014131201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HQX84434.1"
                     /translation="GAHCLEKAQGGRGMLLGGVPGVAPAKVVILGGGVVGTNAAAIAI
                     GMGADVTIIERSTDRMEELVARFGISTKTLYSTQGAIEDECATADLVIGGVLIPGAAA
                     PKLVTKKMLKDWKPGSVFVDVAIDQGGCAETSKATTHADPTYVVDGVIHYCVANMPGG
                     VARTSTFALNNVTLPFAMAIANKGWKKALADDVHLKNGLNVALGKVTYKAVADDLGYA
                     YVPADRVIAG"
     gene            complement(813..1745)
                     /locus_tag="PKV23_03010"
     CDS             complement(813..1745)
                     /locus_tag="PKV23_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin-like domain-containing protein"
                     /protein_id="HQX84435.1"
                     /translation="MSIVTINPALARENFLKRPFELKHGLSDHPLFSLQRLVELAKSL
                     PRDRIEYNSGKVAVGVKPEDVPKIDMPAEDVIRTIETANAWMVIKFVNQDLAYNELLK
                     AFVEEANAAAGKKPGDYCDLQGFIFVSSANSITPFHFDAEENILIQIKGDKFVRTFDN
                     DDRKLVSEETLELTPSRHRNTRYEPWYESRATLHNLKPGDAVHMPYMVPHWVSTGNSY
                     SISMAMTWKTPEVLRLNKIRLINGTLRHYGLPQKAPGVSPGLDALKVMAHDALRALLD
                     PLRKSESIRRFLRGLIYGRSANYYLEGQAKKVGM"
     gene            complement(1742..2854)
                     /locus_tag="PKV23_03015"
     CDS             complement(1742..2854)
                     /locus_tag="PKV23_03015"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQX84436.1"
                     /translation="MLAGNWRMVTAVNLRESADLDAVLPAWKRLADHSLGPAGLNAPE
                     LVMPLLRGLPGAELAVVKQGNDLLFALPIKKRRFMPGLFTNWMTPLTIMGEPHLDREF
                     PETGLTSFINNLKSPILLHSLKVNGSFWNLLARQDAQVSILSSWQRAALSLTGTYETW
                     AESNFGAKRRKEYRRLANRLSEMGRFESLNLEVGRDCSPWVDDLLALEAAGWKGRRGT
                     AIAKDAALQAAFRESCQFLAAAGKLRFWKLALDGKTIAVTYAIVEGNQAWLHKIAYDE
                     AYAKFSPGVLLVLYATERLFAENGIAFVDSCAIPGHPMIENIWRDRIKVSDVMIAPNS
                     IGVKRFAFTLKVEKMRRAARVLARDAYNALRGRKRS"
     gene            complement(2954..3139)
                     /gene="rpmF"
                     /locus_tag="PKV23_03020"
     CDS             complement(2954..3139)
                     /gene="rpmF"
                     /locus_tag="PKV23_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003601373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HQX84437.1"
                     /translation="MAVPRRKMSKSRIGMRRSGQKMQTITWIEDKKSGELRRPHHIDL
                     KTGMYRGRQVLAVKADA"
     gene            complement(3199..3933)
                     /gene="mtgA"
                     /locus_tag="PKV23_03025"
     CDS             complement(3199..3933)
                     /gene="mtgA"
                     /locus_tag="PKV23_03025"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019172110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HQX84438.1"
                     /translation="MTIETQSGMVAAPRRYLDRLKPWQRRLVYALAFVIAWPIVMTLV
                     YTAVPPPFSNLMILRLFTGNGIHKDWVSLSQMSPQLANAVITSEDARFCEHNGVDWVE
                     FQGVVEDVLDDDEGPIRGASTISMQTAKNLFLWDGRSFVRKGLEIPVAYWMDLVFSKR
                     RMIEIYLNIVEWAPGVYGAEAASQFHFRKSAAKLTKREAALLAAVLPNPIKRKAGKPS
                     KRVSSIANRIMSRMNGMGSYLACLGP"
BASE COUNT          833 a         1114 c         1180 g          829 t
ORIGIN      
        1 cggcgcgcat tgcctcgaga aggcccaggg cgggcgcggc atgctgctcg gcggcgttcc
       61 cggcgttgcg ccggccaagg tcgtgatcct tggcgggggt gtggtcggca ccaatgcggc
      121 agcaatcgcc atcggcatgg gtgccgatgt caccatcatc gagcgcagca cggaccgcat
      181 ggaagaactg gtggcccgct tcggcatcag caccaagaca ctttactcca cacaaggggc
      241 cattgaagat gaatgcgcca ccgccgatct cgttatcggc ggagtactca ttcccggcgc
      301 cgctgccccg aaactcgtga ccaagaagat gctgaaggac tggaagcccg gctcggtatt
      361 cgtcgacgtg gccatcgacc agggcggatg tgctgaaacc tcaaaggcca caacccatgc
      421 ggacccaaca tatgttgtgg acggcgtgat ccattactgt gtggccaaca tgccgggcgg
      481 cgtggcgcgc acgtccacct tcgcgctcaa caacgtcacc ctgcccttcg ccatggccat
      541 tgccaacaag ggctggaaaa aggcgcttgc cgatgacgtg catctgaaga atgggctgaa
      601 cgttgccctt ggcaaggtga catacaaggc ggtggccgat gacctgggct atgcctacgt
      661 gccggcggac agggttattg cggggtaaac gatccactcc acttttggga tttcatcgtg
      721 gccgggcttg acccggccat catcttttgg gactcaagaa gatgcccgcg tcgagcgcgg
      781 gcatgatgag aattgaggat gtgtgcttta gattacattc ccaccttttt cgcctgacct
      841 tcgagatagt agttggcact gcggccgtag atcaggccgc gcaggaagcg gcggatgctt
      901 tccgatttgc gcaggggatc aagcaacgcg cgcagagcgt cgtgggccat cactttcaat
      961 gcatccagac ctggcgaaac tcccggcgcc ttctgcggca ggccgtaatg gcgaagcgtg
     1021 ccgttgatca gcctgatctt gttgaggcgc agcacctccg gcgtcttcca ggtcatggcc
     1081 atggaaatcg aataggagtt tccggtgctt acccagtggg gtaccatata gggcatgtgc
     1141 acggcatcgc cgggcttgag gttatgcagc gtggcgcggg attcgtacca aggctcatac
     1201 ctggtgttgc gatgccggga cggcgtcagt tccagagtct cttcggacac cagcttgcga
     1261 tcgtcattgt caaaggtgcg cacgaacttg tcgcccttga tctggatgag gatgttttcc
     1321 tccgcatcaa agtgaaacgg cgtgatcgag tttgccgatg acacaaagat gaaaccttgc
     1381 aggtcgcaat agtcgccggg cttcttgccc gctgccgcat tggcttcttc gacgaaagcc
     1441 ttgagcagtt cgttgtaggc cagatcctga tttacaaact tgatcaccat ccaggcattc
     1501 gccgtttcaa tggtgcggat cacgtcttcc gccggcatat cgatctttgg aacatcctcc
     1561 ggcttcacgc ccacggccac cttgccggaa ttgtattcga tccggtcgcg cggcagcgac
     1621 ttggcgagtt ccaccaggcg ctgcagggaa aacagcggat ggtcggaaag cccatgcttc
     1681 agttcaaagg gccgtttcag gaaattttca cgggccagag cgggattgat ggtgacaatg
     1741 ctcatgatct cttccttccc cgcagcgcgt tataggcatc acgggccaac acccgggccg
     1801 cccggcgcat tttttccact ttcagcgtaa aggcgaagcg cttcacccca attgagttcg
     1861 gtgcaatcat cacgtctgaa accttgatgc ggtcacgcca gatgttttca atcatggggt
     1921 ggccgggaat ggcgcagctg tcgacaaagg caatgccgtt ttcggcaaaa agcctttcgg
     1981 tggcatagag gacaagcagg acaccgggcg agaattttgc gtatgcctcg tcataggcaa
     2041 tcttgtgaag ccatgcctgg ttgccttcga cgatggcgta tgtgacggcg atggtttttc
     2101 catcaagcgc cagcttccag aagcggagct tcccggcggc ggcgagaaat tggcaggact
     2161 ccctgaaagc cgcctgcagt gctgcgtcct ttgcgatggc ggtgccacgc ctgcccttcc
     2221 agccggcagc ttcgagggcc agcagatcgt cgacccatgg gctacaatcg cgcccgacct
     2281 cgaggttgag agattcgaac ctgcccattt ccgaaagccg gttggccagg cgccggtatt
     2341 ccttgcggcg cttggcaccg aaatttgatt cggcccaggt ttcataggtt ccggtcaggc
     2401 tcaatgctgc gcgttgccag gaggagagga tggaaacctg ggcatcctgc ctggccagca
     2461 agttccaaaa tgaaccgttc accttcaggg aatgaagcag aatgggactt ttcagattgt
     2521 taatgaacga ggttaagccc gtttccggaa actcccggtc aagatgaggc tcgcccatga
     2581 tggtgagggg cgtcatccaa ttggtgaaga ggcccggcat gaagcggcgt tttttgatgg
     2641 gaagggcaaa aagcaggtca ttgccctgct tcacgaccgc aagctccgcg cccggaaggc
     2701 ctctcaggag gggcatgacc agttccgggg cattgagccc tgcaggtccc agactgtggt
     2761 cagcgaggcg tttccaggca ggcaaaacgg catccagatc ggcgctctcg cgcagattaa
     2821 ccgccgtcac catacgccaa tttccagcca acatggggct cctgctgcaa aacgcgggcc
     2881 ataccggcgg gcgttgattc aaaaagaaaa agaccggtca aacgaccggt cttaaacctt
     2941 caaatggcag tgattaagcg tcggccttga cggccagaac ctggcggccg cgatacatgc
     3001 cggtcttcag gtcaatgtgg tgaggacggc gcaattcgcc ggatttcttg tcctcaatcc
     3061 aggtaatcgt ctgcatcttc tggcctgaac ggcgcatgcc gatacggctt tttgacattt
     3121 ttctgcgtgg aacggccata acttactcca tatcaggcgg gctcaagccc accacggttg
     3181 ggcggcttat agcgaagctt atggcccaag acaagcgaga taggagccca tgccgttcat
     3241 ccgcgacata atgcggttgg cgatggacga tacccgtttt gagggctttc cggccttgcg
     3301 cttgatggga tttggcagga cggcggcgag caaggcagcc tcgcgttttg tgagcttcgc
     3361 ggctgatttc cggaagtgga actgggaggc cgcctcggcc ccataaacgc cgggggccca
     3421 ttccacgatg ttgaggtaaa tctcaatcat gcggcgcttg gagaacacca aatccatcca
     3481 gtaagccact ggaatttcga gaccttttct cacaaaactg cggccgtccc agaggaacag
     3541 gttcttggcg gtttgcatgg aaatggtgct ggcgccgcgg atcgggccct cgtcgtcgtc
     3601 caggacatct tccaccacgc cttgaaactc cacccagtca accccgttat gctcgcaaaa
     3661 gcgggcgtct tccgaagtta tgacggcatt ggcgagctgc ggcgacatct ggctgaggct
     3721 tacccaatcc ttgtgaatgc cgttgcccgt aaaaagccgg agaatcatca gattagaaaa
     3781 tggcggtggc acggcggtgt aaaccagagt catgacgatg ggccaggcga tgacaaaggc
     3841 taacgcatag accagccgcc gctgccacgg cttcagccgg tcgagatagc gcctgggcgc
     3901 tgcaaccatg cctgattgag tttcaatggt catgacagta cacagggggt ttaaca
//
LOCUS       DAORVN010000103         3932 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_18148_length_3932_cov_3.473046, whole genome shotgun sequence.
ACCESSION   DAORVN010000103 DAORVN010000000
VERSION     DAORVN010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3932)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3932)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3932
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18148_length_3932_cov_3.473046"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            46..522
                     /locus_tag="PKV23_03030"
     CDS             46..522
                     /locus_tag="PKV23_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016918504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02300 family protein"
                     /protein_id="HQX84439.1"
                     /translation="MGASIPSYPISKGSTVVKAELGTKRTCPSCASRFYDLLKNPIVC
                     PKCGVSFIAAPILPSKGDYPGAAAAPAPKPREKVEVEESENADVELISLEDVEEDGKD
                     DDETAGIEDVDLGEDAEPADAEEDDTFLVVEEEEGDNVSGLLDSGPTGGKEGEEEV"
     gene            630..705
                     /locus_tag="PKV23_03035"
     tRNA            630..705
                     /locus_tag="PKV23_03035"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:663..665,aa:Ala,seq:ggc)
     gene            complement(743..1636)
                     /locus_tag="PKV23_03040"
     CDS             complement(743..1636)
                     /locus_tag="PKV23_03040"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HQX84440.1"
                     /translation="MPNKLEISPLTGSFGVIVHNLNLSDISRDKEFPQLRALFEERSA
                     LLIPGQNLSAGDHLRLAEMFGPIEDRKKDERKPGETVEVAMVSNVTDDGGVTGEMDLH
                     TLHLKANQQWHIDSTFLPTPALCNILTAKVVSSEGGQTELASTRAAWTVMPEELKGRI
                     RGRILGHNYRRSRERVSKKLAEQALFNKWPPQRWPAVWSNPVNGRESLYIASHVYEVE
                     GLGQQEGDALIDELINYCTQPQFVYSHTWTVGDVLIWDQRAVLHRGTPWPYDQPRILQ
                     SVCCSLTDGDGLAAARAEIAA"
     gene            complement(1750..3078)
                     /locus_tag="PKV23_03045"
     CDS             complement(1750..3078)
                     /locus_tag="PKV23_03045"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HWE histidine kinase domain-containing protein"
                     /protein_id="HQX84441.1"
                     /translation="MPIPINRMWFEGRLPAFRRSPVIGGLVSAGLVIAAIAIKLLLPI
                     TPSLTILFAVVLVSAWVGGARCAIPTLLACGIAGAYFLSKPLPVQTASWHTVTLAIFL
                     VVGGLVIFVVDLLGKAVDRYQHERRRLDLALKAAKAALWESHPNRPLHWNNNFYDLVG
                     LDYTDEAPPAEQFFAMVHPEDREKMREARRLMDSGLEPRARDEFRLTRPDGKMLWLEN
                     HRTSDPAQKGFYIGIIHDITQRKNAEERVDLVLNELAHRIKNQYAVIIKIAKDTYQQA
                     ETAKDFNEAFQARMMGLARSNDLLLKGGGQEVDLKSLLLSQVETFGDAGRIEVNGPAI
                     AVSPNAAQYLAMAFHELSTNALKHGAFSVETGQVAVTWTVSEEAPSMFRITWKEHGGP
                     APKPKTKSGFGKLVLEKLMPAAVEGTAETEAGPDGITWTLSAPLDHVLRS"
     gene            3273..>3932
                     /locus_tag="PKV23_03050"
     CDS             3273..>3932
                     /locus_tag="PKV23_03050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX84442.1"
                     /translation="MDWEHAIKRNSEVLAGIVETLFVMLGLVGEATVSRISWPAYRAV
                     LRVLRPAESALRRLIVVAAKGLVIAPAVSRPKSAGAVKPGKRGISRPLFQLFDPQPRI
                     VFPRRRNPKRAVPRIHFFNTDGEFITIGPPIRPAKAPARAKSADGMVNAARVIRRLEA
                     LEAALADLPRQARRLVRWRMREEKPRPSAGLPQAGGSPRRRVARRMPVAGLPRHDARH
                     EL"
BASE COUNT          771 a         1164 c         1151 g          846 t
ORIGIN      
        1 acccccttcc catgcggggc tttgacattc ggcaaaaccc gtggcatggg agcctcaatt
       61 ccgtcttatc cgatctcgaa aggatcaacc gtggtcaaag ctgaactcgg aaccaagcgg
      121 acatgccctt cttgcgcgtc ccggttctat gacttgttaa agaatcccat tgtctgcccg
      181 aagtgcggcg tcagcttcat cgcggcgccc atcctgcctt ccaagggtga ttaccccggt
      241 gccgccgcgg cgccggcgcc gaagccccgc gagaaggtgg aggtcgagga aagcgagaac
      301 gccgatgtcg agctcatcag ccttgaggac gtcgaggagg acggcaagga tgatgatgaa
      361 accgcaggca ttgaagacgt cgatctgggc gaagacgccg agcccgccga tgcggaagaa
      421 gacgatacgt tcctcgtcgt cgaagaggaa gaaggcgaca atgtctccgg cctcctggac
      481 agcggcccca cggggggcaa ggaaggcgag gaagaggttt aaccgcttct tcccggtggc
      541 gcgaagtttt gcttgattgt tgaagggcgc ggaactaggt tccgcccgct tcccggacca
      601 gaagatcctg gttcaatatg aagacctgtg gggtcatagc tcagttggga gagcgcttgc
      661 atggcatgca agaggtcgtc ggttcgattc cgtctggctc caccactttc ttcaaatgca
      721 ttccaccctg ctgtgctgct cgtcaggccg caatttccgc ccgcgccgct gccagcccat
      781 cgccatccgt cagggaacag caaacgcttt gcaggattct cggctggtca tagggccagg
      841 gcgtgccgcg atgcagcacg gcgcgttggt cccagatcag gacatcgccc accgtccagg
      901 tatgcgaata cacaaactgc ggttgggtgc aataattgat caactcgtca atcaacgcat
      961 cgccctcctg ttggcccaag ccctcgacct catagacatg tgaggcaatg tagagggatt
     1021 cgcggccatt cacgggattg gaccaaacag cgggccagcg ctgcggcggc catttgttga
     1081 acaatgcctg ctcggccagc ttctttgaca cccgctcgcg cgagcggcga tagttatggc
     1141 caagtatcct gccccgaatg cggcctttca gctcttccgg catcaccgtc caggcggccc
     1201 gcgtcgaggc cagctcggtt tgcccgcctt cagatgacac gaccttggcg gtcagaatgt
     1261 tgcacagcgc aggtgtcggc agaaaggtcg agtcaatatg ccactgctgg ttggctttca
     1321 gatgcagggt atgcaggtcc atttcccccg tcacgccgcc gtcatccgtc acgttgctga
     1381 ccatcgcaac ttcaaccgtt tcacccggtt tgcgttcgtc cttcttgcgg tcctcgatgg
     1441 ggccgaacat ttcagcaagg cgcagatgat cgccggccga aagattctgg cccgggatca
     1501 gcagcgccga acgctcttca aacaaggccc gcagttgggg aaactcctta tcccgggaaa
     1561 tgtccgacag gttcaggttg tgaacaatca ccccgaaact gccggtcagc ggtgatattt
     1621 cgagtttgtt tggcatgtca tatcctctgc gcgcaattct ttaagcacta tgagccgatg
     1681 aatttccaat taaacttgct ggattgcagc aacggacagt tttgggctcg gctgcacagg
     1741 gggattactt caacttcgca gaacgtgatc cagcggggct gatagagtcc acgtgattcc
     1801 gtccgggccg gcctcggttt cggccgtgcc ttcaacggcg gccggcatca gcttttccag
     1861 aaccagtttg ccaaatcctg atttggtttt cggctttggc gcagggccgc cgtgttcctt
     1921 ccaggttatg cggaacatgg agggcgcctc ttccgatact gtccatgtca ccgccacctg
     1981 gccggtttca acagaaaagg cgccatgctt cagggcgttg gttgagagtt catgaaatgc
     2041 catggcgaga tactgcgccg cgtttggcga aacggcgatg gcgggtccgt tcacttcaat
     2101 gcggcccgca tcgccaaagg tttccacctg ggacaagagc aggcttttca gatcaacctc
     2161 ctgaccgccg cctttcagca ggagatcgtt cgagcgggca aggcccatca tgcgggcctg
     2221 gaaggcctcg ttgaagtcct ttgctgtctc cgcctgctgg taagtatcct tggcaatctt
     2281 gatgatcacc gcatattgat tcttgatgcg gtgggccaat tcgttcagca ccagatccac
     2341 ccgctcctcg gcgttcttgc gctgagtaat gtcatggatg atgccgatgt agaatccctt
     2401 ttgcgcgggg tcggacgtcc ggtgattttc cagccacagc attttgccat cgggcctggt
     2461 caggcggaat tcgtcccggg ccctgggttc aagcccgctg tccatgagcc gccgtgcttc
     2521 gcgcattttt tcccggtctt cggggtgaac catcgcgaag aactgctctg ccggcggagc
     2581 ttcgtcggtg taatcgaggc caacgaggtc ataaaaattg ttgttccagt gcagcggacg
     2641 attcggatgg ctttcccaca gcgcggcctt cgccgccttg agcgcgaggt cgagccgcct
     2701 tcgttcgtgc tggtatcggt ccacggcctt gccgagaaga tccacgacga agataaccaa
     2761 cccgcccaca acaagaaaaa tggctagcgt aaccgtatgc cacgaggctg tctgaacggg
     2821 cagcggcttt gacaggaagt aggctccagc gatgccgcat gcgagcagtg taggtattgc
     2881 gcatcttgca ccgcctaccc aagcgctcac cagcacaacg gcaaaaagaa tcgtcaggga
     2941 tggcgtaatg ggaagaagaa gttttatggc aatggcggca ataaccaggc cggcactgac
     3001 gaggccccca atgaccggac tgcgccggaa ggctgggagt cttccttcga accacatgcg
     3061 gtttatggga ataggcatct gtctgctccc tcggacgcaa tataccgaca cggttttgcg
     3121 aatccatggg aatttccccg aacccgcgtc tcagtcagtt tgcgtccaaa gcgctgcgtg
     3181 gatgccagac tgaaaaatat gaatagatcc cccttgacat ctattcctac ataggattat
     3241 gtgttcctgt tttgttcttt cagggataga tcatggactg ggagcacgct atcaaacgca
     3301 acagcgaggt actcgcgggg atcgttgaaa cgctcttcgt gatgctgggg ctggtgggcg
     3361 aagctacggt ttcgcggatt tcgtggcctg cctaccgggc cgtgctgcgt gttttgcgtc
     3421 ccgctgaatc cgccctgcgg cggcttattg tcgttgcggc aaagggcctt gtgattgcgc
     3481 ccgctgtctc acgacccaag tcggcggggg ctgtaaaacc tggaaagcgc gggatttcac
     3541 gtcctttatt ccagctcttc gatccgcagc cccgcattgt gttcccgcgc cgcagaaatc
     3601 ccaagcgggc tgtgccccgc atccactttt tcaataccga tggcgagttt ataaccatcg
     3661 ggccgccgat ccggccggca aaagctcccg cccgggccaa atccgctgat gggatggtca
     3721 atgcggcgcg agtcatccgc cggctcgagg ccctcgaggc tgccttggca gatcttcccc
     3781 gccaggcccg gcgcctcgtc cgctggcgga tgcgggagga aaagccccgg ccgtccgccg
     3841 ggctcccgca ggcgggcggt tcacctcgtc gacgagttgc tcgacgaatg ccagtggctg
     3901 gccttccgcg ccacgatgcc cgacacgagc tg
//
LOCUS       DAORVN010000104         3926 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_18201_length_3926_cov_2.422888, whole genome shotgun sequence.
ACCESSION   DAORVN010000104 DAORVN010000000
VERSION     DAORVN010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3926)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3926)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3926
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18201_length_3926_cov_2.422888"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            245..634
                     /locus_tag="PKV23_03055"
     CDS             245..634
                     /locus_tag="PKV23_03055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF995 domain-containing protein"
                     /protein_id="HQX84443.1"
                     /translation="MNFRACTAILLISLGLAACAGQQDQNFGPPVTAPTGLTQADINN
                     ILSGKSWRWSGPNNSGVTLYASDGTSLVEVTGKGTTTGTWSAKDGQLCEAFAPAPFLP
                     KGVALNCQPMTGSGNVYQVGQATFTLA"
     gene            740..1414
                     /locus_tag="PKV23_03060"
     CDS             740..1414
                     /locus_tag="PKV23_03060"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020184279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HQX84444.1"
                     /translation="MPGAVSRRKLLLGSLSLLSLATPAISQTNLLYGGDGNGFARDPS
                     LLPPPESPVDFKIVPANLKKIPDTFHKQLVVYNGTEWPGTLIVDPERRHLYHVLENQQ
                     AIRYGVGVGREGFSWSGEAKIGMKRRWPRWLPPAEMVVRDENAAKWANGMPGGPDNPL
                     GARALYLYANGEDTLYRIHGTNDPSSIGKAMSSGCVRMLNQDIADLYLRVPVGSKVVV
                     LPSQGV"
     gene            1502..1885
                     /locus_tag="PKV23_03065"
     CDS             1502..1885
                     /locus_tag="PKV23_03065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX84445.1"
                     /translation="MCVAALGLAGCATGPKGVRFIDGAGGAGSKPVAQPIQQFSAGEI
                     QTAIVGKTFQYTRSDGNGFVTYNADGTFNYQDDNKGEGTGKWETSNSLFCEIFGPNAI
                     QECGEFKSTGDAYFAAKSRLVEMKS"
     gene            complement(1882..2526)
                     /locus_tag="PKV23_03070"
     CDS             complement(1882..2526)
                     /locus_tag="PKV23_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007875971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQX84446.1"
                     /translation="MAKLKVLIIPVTPFQQNCSLVFDEESKRGAIVDPGGDVATILNA
                     IEQSNVKIEKILLTHGHIDHAGGAAELREALGVQIEGPHKDDLFLLKDLPMSGAKFGM
                     ADSRSVVPDRWLNDGDDVEFAGLKFTALHAPGHSPGSVVFSNDENRFALMGDVLFQGS
                     VGRTDFPYGSHDTLMASIKEKVLTLGDDVAFLPGHGRASQIGVERKTNPFIQGL"
     gene            2638..3807
                     /locus_tag="PKV23_03075"
     CDS             2638..3807
                     /locus_tag="PKV23_03075"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQX84447.1"
                     /translation="MTATAATPWRYGNLVAAIATIASCDIALGFTFQLLPILMEMQRI
                     PAWIIGLNAAMGPIGILLAGPFLPQLISRFGAKRVAYVAVLFTMAALFAFKAYPSLYI
                     WFPLRFALGIATGALFTISEAWILTFAGPGSRGRIMGVYTSVMAISFSLGPFMLPFTG
                     TEGWLPWLIGISCLGLSILPLTLVNVSNDIFHDEKGGNFLRVVKRAPLLLFAVCTATL
                     FDTVFISFFTIFGLRSGLTLQTASWVLGAGILGNLGFQFLIGALSDRWSRVGVVIWSA
                     VLTIITCVALIFVVHSWLIWPTVIIMATAAFAVYVVALAILGDTFKGADLVAGSAAIS
                     AMWGVGGLVGPPMAGAAIDAFGVNSMPLLLAAFYVMLLVSLALSRGQFIRAPSNG"
BASE COUNT          772 a         1238 c         1022 g          894 t
ORIGIN      
        1 gattgccgcg aaatcgtgac gtctattatt catgctaaca tgttagcatg aataacctct
       61 agaccatatt ggaccaactc ttggtccata aggcatcact tttgtcacgc gttttgcacc
      121 tcaactctta ctaaaagcta cgctcaattc acccgtttgc accacttgca agagagttaa
      181 cgccttctcc acattgtcgc catatgctaa gcgtttacca agggtcattt cggcggagta
      241 acccatgaat tttcgtgcct gcacagctat tctattgatt tcattgggct tggccgcttg
      301 cgccggccag caggatcaga atttcgggcc ccccgtcact gcccccaccg gcctgaccca
      361 ggccgatatc aacaacatcc tgtccggcaa gagctggcgc tggtccggcc ccaacaacag
      421 cggcgtcacc ctctatgcct cggacggcac ctcgctcgtc gaagtcaccg gaaagggaac
      481 aactacaggc acttggagcg ccaaggacgg ccagctctgc gaggccttcg cccccgcccc
      541 cttcctgccg aaaggcgtcg ccctgaattg ccagcccatg accggctccg gcaatgtcta
      601 tcaggtaggc caggccactt ttaccctggc ctgagccctc accacataat cgccacagca
      661 tcttcacgct cccgccgcgg ggaactttca taaggtttct ccgttttaga tcatccattt
      721 gagtcgaaag gagaatgccg tgcctggtgc tgtttcgcgt cgcaaactat tgcttggctc
      781 actttcgctt ttgagccttg caacgcctgc tatttcacag accaatctgc tttacggcgg
      841 agacggcaat ggctttgccc gtgatccaag cctgctgccg ccacccgaat cacccgtcga
      901 cttcaagatc gtcccggcaa atctaaagaa aatccccgac accttccaca agcagctagt
      961 cgtatacaac ggtaccgaat ggccagggac cctgatcgtc gatccggagc gccgccacct
     1021 ctatcacgtc ctcgaaaacc agcaggccat ccgctacggc gtcggcgtcg gccgtgaagg
     1081 tttttcctgg agcggcgagg caaaaatcgg catgaaacgc cgctggccgc gctggctgcc
     1141 gccggcagaa atggttgtgc gcgacgaaaa tgcggcgaaa tgggccaacg gcatgcccgg
     1201 cggcccggac aatcccctgg gcgcccgcgc cctttatctc tatgccaacg gcgaggacac
     1261 cctctaccgc attcacggca ccaatgaccc ctcttcaatt ggcaaggcca tgtcttccgg
     1321 ctgtgtacgc atgttgaatc aggacatagc tgatctctat ttgcgcgtgc ctgtgggttc
     1381 caaggttgtc gtgctgccgt cacaaggggt atagtgcttg ctgcggcagc aaagctgtgg
     1441 cacatggcgt gacgtgggga ctgaactgat cgtgaaacct ggtagcagag cgttgagtat
     1501 catgtgcgtg gccgcgctcg gcctcgccgg ttgtgcaact ggtcccaagg gcgtgcgctt
     1561 catagacggc gcaggcggtg ccggttctaa gcctgtcgcc cagcccatcc agcaattcag
     1621 cgccggcgag atccagaccg cgattgtcgg caagacattt caatacacac gcagcgacgg
     1681 caatggcttt gtcacctaca acgccgatgg caccttcaac taccaggatg acaacaaggg
     1741 cgaaggcacc ggaaaatggg aaaccagcaa cagcctgttc tgcgagatct ttggcccgaa
     1801 cgccattcag gaatgcggtg aattcaagag caccggcgat gcctattttg ctgccaaatc
     1861 ccgcctcgtg gaaatgaagt cttagagtcc ctgaatgaac gggttggtct tgcgttcgac
     1921 gccgatctgg ctggcgcggc catgacccgg cagaaatgcc acatcatcac cgagcgtcag
     1981 cactttttcc ttgatggatg ccatcagcgt gtcgtggctg ccatagggaa aatccgtgcg
     2041 ccccaccgag ccctgaaaca agacatcccc catcagcgca aagcggttct catcgttgga
     2101 gaaaacgact gaccccggcg aatgccccgg ggcatgcagt gccgtgaatt tcaggccggc
     2161 aaactcaacg tcatccccgt cattcagcca gcggtccggc actacgctgc gggaatctgc
     2221 catgccgaat tttgccccgc tcattggcaa atccttcagc aggaaaaggt cgtccttgtg
     2281 cggcccctcg atctgcacgc ccagagcttc gcgaagttcg gcagcaccgc ccgcatgatc
     2341 gatatggcca tgcgtgagga gaattttctc aattttcaca ttcgattgtt caattgcatt
     2401 gagaatggtg gccacatcgc cgcctggatc cacgatggcg ccgcgcttgc tttcttcatc
     2461 gaaaaccagc gaacagttct gctggaaggg cgtgacggga atgataagaa ctttaagctt
     2521 agccatgatg gcgctgcata gcgatcctgc ggattgcccg caagtgcccg cttcccttgc
     2581 ctaaaattgg ggcaacggct agagccagtg aaaaccattc acccgaggca atgccccatg
     2641 accgcaaccg ccgccacgcc ctggcgctat ggcaatcttg ttgcagcaat tgcaacaata
     2701 gccagttgcg atatcgcgct tggcttcacc tttcaattgc tgcccattct catggaaatg
     2761 cagcgcatac cggcctggat cattggcctc aacgctgcca tggggccaat tggcattctc
     2821 ctcgcgggcc ctttcctccc ccagctcatc agccgcttcg gcgcaaagcg tgtcgcctac
     2881 gttgcggtac tgtttacgat ggctgctctg tttgccttca aggcatatcc cagtctctat
     2941 atctggtttc ccctccgctt tgcccttggc atagcaaccg gtgcgctttt cacgatcagc
     3001 gaagcctgga tactcacctt tgccggcccc ggaagccgtg gccgcattat gggggtttac
     3061 accagcgtca tggcaatctc cttttcgctt ggcccgttta tgctgccctt taccggaacg
     3121 gagggctggc tgccgtggct catcggaata tcctgtctgg ggctcagcat ccttcccctc
     3181 acgctggtga atgtcagcaa cgacattttt cacgacgaga aaggcggaaa cttcctgcgt
     3241 gtcgtcaagc gcgcgccact gctcctcttt gcggtttgca ccgccacgct gttcgatacc
     3301 gtgttcattt ccttcttcac catttttggc ttgcgctccg gccttaccct tcaaaccgcc
     3361 agctgggtgc tgggcgccgg catccttggc aatctcggct ttcaattcct gattggcgcg
     3421 ctttccgaca ggtggtcccg cgtcggtgtc gtcatttggt ccgcagtcct tacaattatc
     3481 acctgtgtcg ccctgatctt cgtggtgcat tcatggctca tctggccaac cgtaatcatc
     3541 atggccacgg cagcctttgc cgtttatgtc gtcgcgctcg ccatcctggg cgacaccttc
     3601 aagggggctg atctggttgc cggctctgcc gcgatttccg ccatgtgggg cgtcggcggt
     3661 ctcgtcggcc cgcccatggc aggcgcggca atcgacgcgt ttggcgtgaa ctccatgccg
     3721 ctgctgctgg ccgcattcta tgtcatgctg cttgtcagcc tggctttgag ccgcgggcaa
     3781 ttcatccggg cgccatccaa tggataggca gcgcatcatc aatctctttg ccgccatcag
     3841 cacgatcacc gtattcggtt tcgccctcgg cctgatgttt ccgctcctcg ccctcatcat
     3901 ggaagagcgc ggcatcgatc ccgata
//
LOCUS       DAORVN010000105         3903 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_18382_length_3903_cov_3.878378, whole genome shotgun sequence.
ACCESSION   DAORVN010000105 DAORVN010000000
VERSION     DAORVN010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3903)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3903)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3903
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18382_length_3903_cov_3.878378"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..745
                     /locus_tag="PKV23_03080"
     CDS             <1..745
                     /locus_tag="PKV23_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006330904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HQX84448.1"
                     /translation="PVTLYSSLNLYSKAMIGLEWLSFKTGLGTTNHFEACGFIRSKAG
                     IEYPDIEYHFLPAAMRYDGKVAFKGHGFQVHVGPMRSKSRGHVHVRNSDPKAPPKILF
                     NYMSHPDDWEEFRACIRLTRELFQQPAMLKYAGREIQPGSNVQSDAEIDAFIRQHCES
                     AYHPCGSCKMGSAKDVTAVVDHECRVIGIDGLRVADSSIIPQVTNGNLNAPTLMIGEK
                     ASDHILGRDPLPASNQEPWINPAWKVSQR"
     gene            complement(714..1403)
                     /locus_tag="PKV23_03085"
     CDS             complement(714..1403)
                     /locus_tag="PKV23_03085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX84449.1"
                     /translation="MKYKVRVSPTLLETMTLAAIEAYCYGNLRKQKNTAVETLGYVWG
                     YRKRENGTSIFYLDRTSVSISAERQYHSVTPNLDAAKLKNEVMMRWSPHLTLMGDFHS
                     HPYRNLTEVNDEAGYNFSPEDLKELKSNDFVWEQAGDNPVILVITVCRLGRVRDSVQD
                     NFRNNTARFAVGDFQFWLNAAVGYLDADGKRVVTGNTNSDVHLSVEGWYYNEAGGRVR
                     SASAVRPSRRG"
     gene            complement(1431..2273)
                     /locus_tag="PKV23_03090"
     CDS             complement(1431..2273)
                     /locus_tag="PKV23_03090"
                     /EC_number="2.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018042153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino-acid transaminase"
                     /protein_id="HQX84450.1"
                     /translation="MSRVVYVNGSYVPEEQGKISIFDRGFLFADGIYEVTAVVRSKLV
                     DYDSHMERLERSLREIRLDWPCSKEELKAMHLDMVKRNSLDEGWIYMQITRGSADRDF
                     KFPKGVKPTLIAFTQAKKLVDNPDAIRGVSVITVPDIRWARRDIKSVMLLAPVLGKQA
                     AYEAGAFEAWMVEDGKVTEGTSSNAYIVKDGKVITRAISNNILAGCTRRALFRLAREK
                     GVTLEERAFTPDEAYAADEAFLTSASNFVMPIVEIDKKRVGGGQPGPVTRKLRDMFLE
                     EAMV"
     gene            2395..3750
                     /locus_tag="PKV23_03095"
     CDS             2395..3750
                     /locus_tag="PKV23_03095"
                     /inference="COORDINATES: protein motif:HMM:NF005453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HQX84451.1"
                     /translation="MKTAISEASIKAMLTREKALFIQRNPKSKGHSENAARNWLRGVP
                     MHWMVDWGTPFPLFIERAQGVDLVDADGNSYIDFCLGDTGAMFGHSPKPVVDTLLREG
                     AHGFTTMLPSASAVKVGQLLEDRFKLPAWQITATASDANRAVLRWCRAITGRKKILVF
                     NHCYHGAVDETYVNLDHGKPEADRALIGEPNDLTQFTKVIEFNDIPALEAALAPGDVA
                     CVLAEPIMTNVGMVLPDEGFHSALREITRRTGTLLIIDETHCMSSGPGGYTGEHGLEP
                     DAFVLGKPIAGGIPAAVYGFSESVTARIRDYLKSRSPGHSGIGTTLAGSKIQLALMET
                     VLETYFTREAFAPLIALAKKLEKGLADVIIKHSAPWHVVRAGARVEFMCCPNRPRNGG
                     EAAKMIHQPIDQAVHHHLLNRGIIMTPFHNMLLICPSTTEAHVNQMIEGLDHCLAELM
                     S"
BASE COUNT          829 a         1267 c         1024 g          783 t
ORIGIN      
        1 gcctgtcacc ctctattcaa gcctcaacct ctactccaag gccatgatcg gcctggaatg
       61 gctgtcgttc aagactggcc tgggaaccac caatcacttt gaagcctgcg gcttcatccg
      121 ctccaaggcc ggcattgaat atcccgacat cgaataccac ttcctgcccg ccgccatgcg
      181 ctatgacggc aaggtggcct tcaagggcca cggcttccag gttcacgtgg ggcccatgcg
      241 cagcaagtcg cgcggccatg tccatgtgcg caatagcgac cccaaggcgc cgcccaaaat
      301 tctgttcaat tacatgtccc atcccgatga ctgggaggag ttccgcgcct gcatccgcct
      361 cacccgcgaa ctgttccagc agccggccat gctgaaatac gccgggcgtg aaatccagcc
      421 cggaagcaat gtgcaatccg atgcggagat cgatgccttc atccgccagc attgcgaaag
      481 cgcctatcac ccctgcggct cctgcaagat gggttcggcc aaggatgtga ccgccgtcgt
      541 cgatcatgaa tgccgcgtca tcggcatcga tggcctgcgc gtcgccgaca gttccatcat
      601 tccgcaggtt accaacggca atctcaatgc gccgaccctg atgataggtg agaaagcctc
      661 cgaccacatc ctcggccgcg atccgctgcc ggcttcgaac caggagccct ggatcaaccc
      721 cgcctggaag gtctcacagc gctagccgag cgcacccgcc cgcccgcttc gttgtagtac
      781 cagccctcga cgctcagatg gacatccgag ttggtgttgc cggtgaccac gcgcttgcca
      841 tccgcatcga gataacccac cgccgcattc agccagaact gaaaatcgcc caccgcgaag
      901 cgcgccgtgt tgttgcggaa attgtcctgc acgctgtcgc gcacccggcc cagccggcac
      961 accgtgatca ccaggatcac cggattgtcg cccgcctgct cccagacgaa atcattgctc
     1021 ttcagttcct tgagatcttc cggcgaaaaa ttatagccgg cctcgtcgtt gacctcggtc
     1081 aggttgcggt agggatggct gtggaaatcg cccatcaggg tgaggtgcgg cgaccagcgc
     1141 atcatcacct cgttcttcag ctttgccgca tcgagattgg gggttacgct gtgatactgc
     1201 cgttccgccg aaatgctgac gctggtgcgg tcgagataga agatcgaggt gccgttctcg
     1261 cgcttgcggt agccccagac atagccaagg gtttcaaccg ccgtgttctt ctgcttgcgc
     1321 agattgccat aacaataggc ttcgatagcc gccagtgtca tggtttccaa tagtgtcggc
     1381 gatactcgga ccttgtactt catttctatc ccaaacaaac ctgaccaaaa ttaaaccatt
     1441 gcttcttcca ggaacatgtc ccgcaatttg cgcgtgaccg gccccggttg cccgccgccc
     1501 acccgcttct tgtcgatctc gacaatcggc atcacgaaat ttgacgcgct cgtcagaaag
     1561 gcctcgtccg ccgcataagc ctcgtccggc gtaaaggcgc gctcttcaag cgtcacaccc
     1621 ttttccctgg ccaggcggaa cagggcccgc cgcgtgcagc cggccaggat attgttcgag
     1681 atggcccggg tgatcacctt gccgtccttg acgatatagg cgtttgacga cgtcccttcc
     1741 gtcactttac catcctcgac catccacgcc tcaaacgcgc ctgcctcata cgccgcctgc
     1801 ttgcccaaca ccggggcaag cagcatgacg gacttgatgt cccgcctcgc ccagcgaatg
     1861 tctggcaccg tgatcacgct cacccccctg atggcatccg gattatcaac caatttcttg
     1921 gcctgtgtaa aagcaatgag tgtaggctta acgcctttgg ggaacttgaa atcccggtcg
     1981 gcggagcccc gggtaatctg catgtaaatc cagccttcat caagactgtt gcgcttaacc
     2041 atatccagat gcatggcttt aagctcctcc ttgctgcacg gccagtccag gcggatttcg
     2101 cgcaaagacc gctccagccg ctccatgtgg gaatcatagt caaccagctt ggaccttacc
     2161 accgcagtta cctcatagat gccatccgca aacaggaagc cgcggtcaaa tatcgagatt
     2221 ttcccttgtt cttcagggac atagcttccg ttaacgtaaa cgacgcggct cattcagggc
     2281 tccagattgc tgcactgcat tcaaagcaga ttcgggtcaa aaaatccacg ggggttgttt
     2341 atgggcctaa agcccggcta aaaccaaggg caacgtccac ttgccaagga aatcatgaaa
     2401 actgccatat cggaagcctc gatcaaggcc atgctgacga gagaaaaggc cctcttcatc
     2461 cagcgcaacc caaagtccaa ggggcattcc gaaaatgcag caaggaactg gctgcgcggc
     2521 gtgcccatgc attggatggt ggactgggga accccctttc cgctgttcat cgagagggcc
     2581 cagggcgtag atttagtcga cgccgacggc aacagctaca ttgatttctg cctcggcgac
     2641 acgggcgcca tgttcggcca ttcgcccaag cctgtggtcg ataccctgct gcgcgaaggg
     2701 gcccatggct tcaccaccat gctgccgtcg gccagtgccg tgaaggtggg ccagctcctc
     2761 gaagatcgat tcaaattgcc ggcctggcag atcaccgcaa cggcgtctga tgccaaccgc
     2821 gcggtgctgc gctggtgccg cgccataacc ggccgcaaga aaatcctggt cttcaaccac
     2881 tgctatcacg gcgccgtgga tgaaacctat gtgaaccttg accatggcaa gccggaagcc
     2941 gaccgcgccc tcatcggcga acccaatgac ctcacccagt ttaccaaggt catcgagttc
     3001 aacgatattc cggcccttga agcagccctc gcccccggcg acgtggcctg cgttctggcc
     3061 gagcccatca tgaccaatgt cggcatggtc ctgcccgacg agggcttcca ctcggcactg
     3121 cgtgaaatca cccgccgcac cgggaccctg ctcatcatcg atgaaaccca ttgcatgtca
     3181 tcggggcccg gcggctacac cggcgagcat gggctcgagc ccgatgcctt tgtgctggga
     3241 aaacccattg ccggcggcat tcccgctgcc gtctatggtt tttccgaaag tgtcacggcc
     3301 cgcatccggg attacctgaa aagccgttcg cccggccatt ccggcatcgg cacgacgctt
     3361 gccggatcga agatccagtt ggccctgatg gaaaccgttc tcgaaaccta tttcacaagg
     3421 gaggccttcg cgcccctgat cgcgcttgcc aaaaagctcg aaaagggact ggcggatgtc
     3481 atcatcaaac attcagctcc ctggcatgtg gttcgcgccg gagcccgcgt tgaattcatg
     3541 tgctgtccaa atcgcccgcg aaatggcggc gaggcggcga agatgatcca ccagcccatc
     3601 gaccaggcag tgcatcatca cctcttgaac cgcggcatca tcatgacgcc gtttcacaac
     3661 atgctgctca tctgcccctc aaccacggag gcacacgtca accaaatgat cgagggactc
     3721 gatcactgcc tggcggaact gatgagctaa accggagaca gtaacatgtc gattgtattt
     3781 ccaaggcgcg aggcggaagc ccacgcattt ttagacgcaa accccgacat caccagcatc
     3841 cacgtgatct ggaccgatat ctgcggcgtt gcccgcggca agatcctgag gcgcgatgaa
     3901 ctg
//
LOCUS       DAORVN010000106         3900 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_18403_length_3900_cov_2.735241, whole genome shotgun sequence.
ACCESSION   DAORVN010000106 DAORVN010000000
VERSION     DAORVN010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3900)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3900)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3900
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18403_length_3900_cov_2.735241"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..920
                     /locus_tag="PKV23_03100"
     CDS             <1..920
                     /locus_tag="PKV23_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011995344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HQX84452.1"
                     /translation="LARLLETKNPLFVEWFAIDQIYGREADVGLHSHWVDPTEPLALA
                     VLKNADGVIITSATLKDRPPDVPDDWANAEMRTGAVHLPYPVKRASYDSPFDYASTCR
                     LLVVNDVNREDMDQVAAAYRELFFASRGGGLGLFTAISRLRAVHKRLSVALAQKGLAL
                     YAQHVDPMDTGTLVDMFRAERDACLLGTDAVRDGVDVPGDSLRLIVLDRVPWGTPTIL
                     ERARRETFGGNAYTDMVVRLRLRQAFGRLIRSENDRGCFVILDPRLASRFTTAFPPGL
                     AVERVGLVEAIERVRTFTAVSLDKSPARG"
     gene            947..1360
                     /locus_tag="PKV23_03105"
     CDS             947..1360
                     /locus_tag="PKV23_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013419857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HQX84453.1"
                     /translation="MPGTISTEQALIYVMVTMSGVEGKINATELKEIGRVVKSLPIFK
                     NFDTARLTTVAQECGEMLQEPEGLMAVLGLARDQLSPKLRETAYALAAEVAAADLAVG
                     KEELRFLAILRDIFGLDKLVTAALERAVIARYQTL"
     gene            1362..2321
                     /gene="pip"
                     /locus_tag="PKV23_03110"
     CDS             1362..2321
                     /gene="pip"
                     /locus_tag="PKV23_03110"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020188058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HQX84454.1"
                     /translation="MRQFFPELQPYTRGRLRVSELHEIYYEECGNPKGKPVVVLHGGP
                     GGGISPFLRRLHDPRLYRIILFDQRGCGQSTPYAELRENTTWHLVADIEALRAHLGIE
                     TWQVLGGSWGSTLALAYAQSHVERVTELILRGIFAIRKREIDWFYQEGASMLFPDVWE
                     TYLAPIPVPEREDLVAAYYRRLTGTDSHEQMACAKAWSQWEASTLSLRPNPQLISEFG
                     GDAFALAFARIECHYFVNKGFFDRDGQLLADAPKLRAIPGVIIQGRYDVVTPMDTAWS
                     LYRAWPEAAFEIIPDAGHTATEPGITDALIRATDRFAMRAPHS"
     gene            2415..2540
                     /locus_tag="PKV23_03115"
     CDS             2415..2540
                     /locus_tag="PKV23_03115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX84455.1"
                     /translation="MTGVFKEIVFGLGRGFGIYFSQRHEFIDSVMTPGRPVKKRR"
     gene            2882..>3900
                     /gene="rmuC"
                     /locus_tag="PKV23_03120"
     CDS             2882..>3900
                     /gene="rmuC"
                     /locus_tag="PKV23_03120"
                     /inference="COORDINATES: protein motif:HMM:NF014680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HQX84456.1"
                     /translation="MQLDQIILQIGSEAFTLLDVLLAGAALALVLILATLIVTWRAQR
                     NRKSETLEAMRRASELEYRLAEMAGSLRNFADQSQGAQAHLTRTLDERLDHVGQRIGL
                     GLTDQAERTTQSLTHLYERLAVIDAAQKNLTSLSTEMISLKDILSNKQARGAYGQGRM
                     EAIVRDGLHARAYAFQPTLSNGMRPDCLISLPDSELKLVIDAKFPLEAFNSLREAKGE
                     GDARTAEARLRGDVSKHVKDIAEKYLITGETHETAIMFVPSESIYADLHEKFEDVIQK
                     AHRARVIIASPNVLMLLIQTMQAIFKDSAMREQAHVIKIEVMRLLEDVGRLKERVGDL
                     QKHFG"
BASE COUNT          732 a         1175 c         1217 g          776 t
ORIGIN      
        1 tgctggcccg gctccttgaa acgaagaacc ccctgttcgt cgaatggttc gccattgacc
       61 agatctatgg ccgtgaggcc gacgtggggt tgcattccca ctgggttgat ccgacggagc
      121 ccttagcgct ggcagtgctg aagaacgccg atggcgttat catcacttcg gccacgctca
      181 aggaccggcc gcccgatgtg cccgatgact gggccaatgc ggaaatgcgc acgggtgcgg
      241 tgcacctgcc ctatccggtg aaacgcgcga gctatgactc gccctttgac tacgcaagta
      301 cgtgccggct gctggtggtg aatgatgtga accgcgagga catggaccag gttgccgccg
      361 cctaccgcga actgtttttc gccagccgcg gcggagggct ggggctgttc acggcgattt
      421 cacggctgcg cgcagtgcac aagcgcctga gcgtcgcgct ggcccaaaag ggcctggcgc
      481 tttatgcgca gcatgtggat cccatggata cgggaacgct ggtggacatg ttccgggccg
      541 agcgcgatgc ctgcctgctg gggaccgatg cggtgcgtga cggagttgat gtgcccggtg
      601 actcgctgcg gctcatcgtg ctggaccggg tgccatgggg aacgcccacc attctggagc
      661 gggcgcggcg ggaaaccttc ggcggcaatg cctatacgga catggtggtg cggctgcgac
      721 tgcgccaggc ctttggccgc ctgatccgca gcgagaatga ccggggctgc tttgtcatcc
      781 tcgatcccag gctggcctca cgctttacca cggcgtttcc gccgggattg gcggtggagc
      841 gggtggggct ggttgaggcg attgagcggg taaggacctt taccgcagtt tcactcgaca
      901 aaagcccggc caggggctag ccagcgtcca acatttggag aacgacatgc ccggcacgat
      961 cagtacggaa caggcgctta tttatgtcat ggtcaccatg tcgggcgtcg aaggcaagat
     1021 caacgcgacg gaattgaagg agattggccg ggtggtgaaa agcctgccga tcttcaagaa
     1081 tttcgatacg gcgcggctga caacggtggc ccaggaatgc ggcgagatgc tgcaggagcc
     1141 ggaagggctg atggccgttc tcgggctggc gcgcgaccag ctttcgccga agctgcgcga
     1201 gacggcctat gcccttgcgg cagaagtggc ggcggcggat ctggcggtgg gcaaggaaga
     1261 actgcgcttc ctcgccatcc tgcgtgacat cttcggcctc gacaagctgg tgacggcggc
     1321 actcgagcgc gcggtcatcg cgcgttacca gacgctctga catgcggcaa ttctttccgg
     1381 aactgcagcc ctacacccgt ggccggcttc gcgtctcgga gctccatgag atctattacg
     1441 aggaatgcgg caacccgaag ggcaagccgg tggtcgtgct gcatggcggg ccgggcgggg
     1501 gcatttctcc gttcctgcgc cgcctgcacg atcccaggct ttaccgcatc atcctcttcg
     1561 accagcgggg ctgcgggcaa tccacgccat acgcggaatt gcgcgagaac accacctggc
     1621 atctggtggc tgacattgaa gccttgcggg cccatctcgg aatcgagacg tggcaggtgc
     1681 tcggcggttc atgggggtcg acgctggcat tggcctatgc gcaaagccat gtggagcggg
     1741 tgaccgagct tattcttcgc ggcattttcg ccatccgcaa gcgcgagatc gactggttct
     1801 accaggaggg cgcgagcatg ctgtttcccg atgtctggga aacctacctg gctcccattc
     1861 ctgtcccaga gcgcgaggat ctggttgccg cctattaccg gcggctgacc ggcaccgaca
     1921 gccacgagca gatggcctgc gccaaggcct ggagccagtg ggaagccagc actctttcgc
     1981 tgcgccccaa tccccaactc atcagcgaat tcggcgggga cgccttcgcc ctggcctttg
     2041 cccgcattga gtgccactac ttcgtcaaca agggcttctt tgaccgggac ggtcagttgc
     2101 ttgcagatgc tcccaagctc cgggcaattc cgggcgtgat catccagggc cgctacgatg
     2161 tggtgacgcc catggacaca gcctggtccc tgtaccgggc ctggccggaa gcggccttcg
     2221 agatcattcc cgacgcgggc catacggcca cggaaccggg aatcaccgac gcgctgattc
     2281 gggccacgga ccgattcgcc atgcgggcgc cgcatagctg agtttcgtgc aattcacctt
     2341 gaatctctat atttcgttca tatttcaatg gcatagcacg ttgattcagc agtctattcg
     2401 ttaaggaggt ctttatgacc ggtgtattca aggaaatcgt attcggcctg ggccgcggat
     2461 ttggcatcta tttctcccag cgccacgaat ttatcgactc ggtcatgacc ccgggtcgcc
     2521 ccgttaagaa gcgccgctaa gcggttgttc ttaatcgtta ttgtttcggc gtggaaagtt
     2581 tcggtgggga tctcaccttg ataccgccgc aatcttgcct gcgcaatgcc tcgttctgcc
     2641 cgtgatcaag ccatgatcga aagccactct cacactcaat ccagcgggga ttagccagcc
     2701 taaacagctg gttcttgaga gggaaatttc gggaacctaa taaagctacc cctgtttgtg
     2761 agcgagttga cctgtgcggc tcctgtcgcc gtcccgagtt gtgcgggtca agggcccgtc
     2821 cacgcttagt tgagtgtgga cgggccacgc ttctttcccc gaatcggggt aaaagattct
     2881 catgcaactc gaccagatca ttcttcaaat cggctccgag gcctttaccc tgcttgatgt
     2941 cttgctggcg ggggctgccc tggccctggt cctcattctt gccaccctga tcgtcacgtg
     3001 gcgggcccag cgcaaccgga aaagtgagac tctcgaagcc atgcgtcgcg cctcggagct
     3061 ggaataccgg ctggccgaga tggcggggtc gctgcgcaat tttgccgacc agtcgcaggg
     3121 cgctcaggca caccttacac gcaccctgga tgagcggctg gaccatgtgg ggcagcggat
     3181 cggccttggc cttacggacc aggcggaacg caccacgcag tccctcaccc atctctatga
     3241 gcgcctggcg gtcattgacg cggcccagaa gaacctcacc tcgctttcca ccgagatgat
     3301 ctcgctcaag gacattctat ccaacaagca ggcgcgcggc gcctatggcc aggggcgcat
     3361 ggaggccatc gtgcgcgacg ggcttcatgc ccgggcctat gccttccaac ccacgctcag
     3421 caacgggatg cggccggatt gcctgatcag cctgccggat tcggagctca agctggtgat
     3481 cgatgccaag ttcccgctgg aggccttcaa ctccctgcgc gaggccaagg gtgagggcga
     3541 tgcccggacc gcagaagcac ggctgcgggg cgatgtttca aagcatgtga aggacattgc
     3601 cgaaaagtac ctgatcaccg gcgagaccca cgagacggcc atcatgttcg tgccgtccga
     3661 atccatatat gcggacttgc acgaaaaatt cgaagacgtc atccagaagg cccaccgggc
     3721 gcgcgtcatc atcgcctcgc ccaatgttct catgctgctc atccagacca tgcaggcaat
     3781 cttcaaggac tcggccatgc gcgagcaggc ccatgtgatc aagatcgaag tcatgcgcct
     3841 gctggaagac gtggggcggc tcaaggaacg ggtcggcgac ctgcaaaagc atttcggcaa
//
LOCUS       DAORVN010000107         3886 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_18532_length_3886_cov_3.022971, whole genome shotgun sequence.
ACCESSION   DAORVN010000107 DAORVN010000000
VERSION     DAORVN010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3886
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18532_length_3886_cov_3.022971"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1028
                     /locus_tag="PKV23_03125"
     CDS             <1..1028
                     /locus_tag="PKV23_03125"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HQX84457.1"
                     /translation="AARRAGARARRGDDRRRRGRADADAAGNADRVLSRHGRRHADLR
                     HPHARIGHATSHDLMTWKYEGIVLGPSEQAGWDDGTTWTGSVVKSPDGKWMMFYTGTQ
                     LSESRKYQRIGAAVSDDLFTWTRLGRAPLLEVDPEIYETYDPSRWHDQAFRDPWVYPD
                     PGGHGWRMIFTARVKDGLPGGSGVIGQASSPDLLTWKAEKPLFSIGHYGEMEVPQIFE
                     LNGWWFCLFSNATRHMEPAYLATGKTGVVTGTHYIRSREPSGPFELVEDRFFGGDAIG
                     SFYGGRFLRTPAGTPVFLAFLNHRADGSFVGAISDPMPIWTTSEGYLRIDGRQYGMAL
                     QSEMSIA"
     gene            1187..2800
                     /locus_tag="PKV23_03130"
     CDS             1187..2800
                     /locus_tag="PKV23_03130"
                     /inference="COORDINATES: protein motif:HMM:NF017081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HQX84458.1"
                     /translation="MRGIVLHVVLFLAYLVLFRASYIFQTSDLRSTPWNPETGIAVVA
                     GALLGWASLPAIFLASLVSKTLNPTILSFGWEIVAAAVNAMVFAGSAAALRRFLPSIN
                     TATTANVILFICYAFVATLASAVARLFIAALALDIYPVYLINYTFTLSVGNVVGILTI
                     VPVFFVSRPVADWMRYFSGFRAMHWLTTLIILLTSVIVFGLEDTDKFKFFYLVFLPVM
                     AFAIKDGLMGAAYAILLSDTAMIAILLWRVSEPSTATELQVLMISLSATGLLMGATIS
                     ERRRISTQLEESHVRLRDTQAALLQASRISLASEMAAALAHEINQPLSSIRSFVRTAR
                     RKLDKGKVNYAELKSDIDSAVKEVDVAANLIRETRKFLERGDVRKDRINLRQLLVTCS
                     DLIAPELGYSKIAWELHMPERLPTVIGNAMQIQQVVLNLVRNAREAILDGRSKMRKIE
                     LSVSLESRPGHVEIAVSDSGPGIQSEVKAMLFKPLKSSKVNGLGLGLSLSNTIVTSHG
                     GSIWYDEASSTVTRFAFTLPLSASKESER"
     gene            2812..3408
                     /locus_tag="PKV23_03135"
     CDS             2812..3408
                     /locus_tag="PKV23_03135"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQX84459.1"
                     /translation="MIIDDEQSVRDSLSAVLATYGFRTASCGNAHEALEMIRKSTPDC
                     VVLDVRMPEIDGLALQRMLAQTAANLPVIIITGHADIAMAVQAMRNGASDFIEKPIDD
                     EQLVASIQAAIDQKRHASRSGPDEKSLLARLATLTERERSVAEMVAEGYSSAAIAAAF
                     SISVRTVDHHRASILAKMQATSLPQLIRYLLRILPPKT"
     gene            3622..>3886
                     /locus_tag="PKV23_03140"
     CDS             3622..>3886
                     /locus_tag="PKV23_03140"
                     /inference="COORDINATES: protein motif:HMM:NF012920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HQX84460.1"
                     /translation="MDLTAVQDARAINVYGNGMLPLYRHGDQLIVSAKVPVKAGDRIL
                     LELKDGSHVCGTLSRKTHQLVGINIGGQSRKEALIDTSRIAFLG"
BASE COUNT          820 a         1066 c         1106 g          894 t
ORIGIN      
        1 aggcagcgcg tcgtgccggt gctcgagcgc ggcgaggcga tgatcgccgc cgccggggac
       61 gtgccgacgc cgacgctgct ggcaacgctg atcgagtcct ttcccgacat ggtcgtcgcc
      121 acgccgatct ccgccatccc catgcccgca taggccatgc gacttcgcat gatctcatga
      181 cctggaaata cgagggaatc gtgcttgggc cttctgaaca ggctggctgg gatgatggaa
      241 cgacctggac tggttcagtc gtgaaaagtc cagacggaaa gtggatgatg ttctacacgg
      301 gaacacaatt gtccgagagc agaaaatacc agcgcattgg cgcggcagtg tcagatgacc
      361 ttttcacctg gaccaggctt ggcagagcgc ccttgctgga agtcgatccc gagatctacg
      421 agacttatga tccgtcacgc tggcacgacc aggcatttcg cgacccatgg gtttatccag
      481 atccgggcgg ccatggctgg cggatgatat tcacggcccg cgtgaaggat gggctgcccg
      541 gcggcagcgg tgtaattggc caggcctcat cgcctgacct gcttacatgg aaagccgaga
      601 agcccctttt cagcattggc cactacggcg aaatggaagt gccgcagatc tttgaactga
      661 atggctggtg gttttgcctt ttcagcaacg ccacacggca catggaacct gcgtacctgg
      721 cgaccggaaa aaccggcgtg gtgacgggca cacattacat ccggtccaga gagccctcgg
      781 ggcctttcga actggttgaa gacaggtttt tcggcggcga cgccatagga agcttctatg
      841 gcggccgctt cctccggaca ccggcgggaa cccccgtttt cctggcattt ctgaaccacc
      901 gggccgatgg aagttttgtc ggggcaatct ccgatccgat gccgatttgg accacaagcg
      961 agggttattt gcgcattgac ggcaggcaat atggcatggc gctgcagagt gagatgtcga
     1021 tcgcttgatt gcaacacctg aggcgaacaa attccgtgtt tggaaagact gtgtacctgg
     1081 actagctttg acagatactg tgacggagga attgccagaa ttgccaggca gtaaagctta
     1141 cgagtaccga atcccattcc tgccggcggt ccaagtcagg gtgaccatgc gcgggatagt
     1201 cctgcatgtg gttctatttt tagcctacct ggtgcttttc agggcgtcat acatttttca
     1261 aacctctgac ctccgatcaa cgccctggaa tcccgagacc ggcattgccg tggttgctgg
     1321 cgcgcttctc ggctgggcaa gtctgccggc gatcttcctc gcgtcgcttg tcagcaagac
     1381 attgaacccc accatcctct ctttcggatg ggaaattgtt gcggccgccg ttaacgccat
     1441 ggtttttgcg ggttctgctg ccgccctgcg tagattcctg ccctccatca acacggcgac
     1501 gacggcaaat gtcatcctgt tcatctgcta tgcctttgtt gcaacgctgg cgtccgcggt
     1561 tgcgaggctg ttcattgccg cattggccct ggatatctat ccggtctacc tgatcaacta
     1621 tactttcact ctgtcagttg gaaacgttgt cggcatcctc accatcgtgc ctgttttttt
     1681 tgtttcaagg cctgttgccg attggatgag atacttttca ggtttccggg ccatgcattg
     1741 gctgacaacg ctcatcatac tgctgacatc agtgatcgta tttggccttg aggacaccga
     1801 caaattcaag ttcttctacc tcgtcttcct gcccgtcatg gctttcgcga tcaaggatgg
     1861 cctcatgggc gccgcctatg ccatcctcct gagcgatacc gcgatgatcg cgatcctgct
     1921 ttggcgtgtt tcggaacctt ccacagccac agaactacag gtgttgatga tctcgctttc
     1981 ggcaaccgga cttctcatgg gcgcaacaat ttccgagcgg cggcggattt caacgcagct
     2041 tgaggaaagc cacgtgcggc tgcgcgatac gcaggcggcc ctcttgcagg cttcgcgtat
     2101 atcactggcg agtgaaatgg cagccgccct ggcgcatgaa atcaaccagc ccctgtcgtc
     2161 catccgcagt ttcgtgcgaa cggcacggcg caagctcgac aagggcaagg tgaattatgc
     2221 ggagctcaag tctgatattg attcagcggt gaaggaagtt gacgtcgccg ccaacctcat
     2281 tcgtgaaacc agaaagtttc tggagcgcgg agacgtccgc aaggacagga taaacctgcg
     2341 gcaattgctg gtcacctgtt ccgatttgat cgcccctgag ctgggttatt cgaaaatcgc
     2401 ctgggagctc cacatgccgg aaaggctgcc gaccgtcatt ggcaacgcca tgcaaattca
     2461 gcaggttgtc ttgaatctgg tccgcaatgc cagggaagcc atactggacg gacgatccaa
     2521 gatgcgaaaa atcgaattgt ccgtgtcgtt ggaatcacgg ccaggccatg tcgaaattgc
     2581 tgtctcggat tcagggcctg gaattcaatc agaggtgaaa gccatgcttt tcaaaccctt
     2641 gaagtcgtcc aaggtcaacg gtctgggttt ggggctctcg ctcagcaata ccattgtaac
     2701 atcccacggt ggaagtatct ggtatgatga ggcctcatcc actgtaacca ggtttgcatt
     2761 tacgctccct ctctcagcca gcaaggagag cgagcggtga agcaccgggt catgatcatc
     2821 gacgacgagc aatcagtccg tgactctttg tccgctgtgc tggcaactta tggcttcaga
     2881 accgcttcat gtggcaatgc gcacgaagcc ttggaaatga ttcgaaaatc cacaccggac
     2941 tgtgtggttc tggatgtccg tatgccagaa atagatggct tggcattgca gcggatgctt
     3001 gcgcaaactg cggccaacct tccagtcatc atcataactg gccatgccga tatcgccatg
     3061 gccgttcagg ccatgcgaaa tggcgcctct gattttatcg agaagccgat tgacgacgag
     3121 cagctggttg catcgatcca ggctgccatc gaccagaaac ggcacgcttc aaggtcgggg
     3181 cccgatgaaa aatctcttct ggcccgcctc gcaactctga ctgagcggga gaggtccgtg
     3241 gcggaaatgg tagccgaagg ctattcgagt gcagccattg cagcggcatt ctccatcagt
     3301 gtacggaccg tcgaccacca ccgggccagt atcctggcga agatgcaggc aacgtcattg
     3361 ccgcaactta ttaggtatct gctcaggatt ttgccgccga agacctgaaa ttgtcggacc
     3421 gcacatcagt cttggagagg ggtgcttgcg ggagctgatg tgcggtccgg tatttcccga
     3481 ttgtgtgtac tgggttcggg gtacccagac tgcggggcag ggtcaatctg cctttcagag
     3541 cccggcaagc caataggtag tgctacctat gtcgccggat ttcactggcg ctatcttcga
     3601 aagtcagtgg ggagaactga tttggatttg actgccgtcc aggatgccag ggctatcaat
     3661 gtatacggta atggaatgtt gccgctctat cgtcatggcg accagttgat cgtttcggcg
     3721 aaagtccccg tgaaagcggg cgacaggatc ttgcttgaat tgaaggacgg cagccatgtt
     3781 tgtggcaccc tgtcgcgcaa aacccaccaa ttggtgggga tcaacattgg cgggcagtcg
     3841 cgcaaggaag cactgatcga cacatccaga atagctttcc tcggcc
//
LOCUS       DAORVN010000108         3882 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_18569_length_3882_cov_2.936242, whole genome shotgun sequence.
ACCESSION   DAORVN010000108 DAORVN010000000
VERSION     DAORVN010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3882
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18569_length_3882_cov_2.936242"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /gene="mlaD"
                     /locus_tag="PKV23_03145"
     CDS             complement(<1..407)
                     /gene="mlaD"
                     /locus_tag="PKV23_03145"
                     /inference="COORDINATES: protein motif:HMM:TIGR04430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HQX84461.1"
                     /translation="MNNSTVETLIGAVVIAIAGAFLVFAYNTPGMGQKGGGYLISAEF
                     DNVEGVNVGTDIRMAGIKVGTVTAQSLNPENFQALVTMSIDPAVQLTDDTSAKVTSEG
                     LLGSKFISLEPGGSETKLTEGGVIAYTQGAVDI"
     gene            complement(497..895)
                     /locus_tag="PKV23_03150"
     CDS             complement(497..895)
                     /locus_tag="PKV23_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010257 - NADH dehydrogenase complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone oxidoreductase subunit NDUFA12"
                     /protein_id="HQX84462.1"
                     /translation="MHFLKQFFTWWSGQTLATRFFTWRKGEHVGTDQAGNHYYRAKSA
                     MPDSIAERRWVIFNGYSEASSIPPGWHGWMHHRVDKVPSSSDAPDREWQKPHQANLTG
                     SAGAYRPPGSIVGAARPKKSDPGYQAWRPD"
     gene            complement(975..2987)
                     /locus_tag="PKV23_03155"
     CDS             complement(975..2987)
                     /locus_tag="PKV23_03155"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HQX84463.1"
                     /translation="MDHIQPFLDYFARNPEWAIVVVFLIAFGEALLIIGLFVPSTAVL
                     VGAGVLVGSGHLPFWPVFLATLIGAVAGDQISYWAGRFFGEHLKILWPLNRFPQLVAR
                     GEEFVRNHGGKSIALGRFVPGVKAVVPGIVGMLGMSQLYFATVNISSGIVWTIAHVLP
                     GILIGQGLALAGELSGHLGLVLVALLVILAVAGWLIRLFAAGVSPRLDHILQRVSAWA
                     KTRNNRSLHRFGRAVAPENPRSLLIIFFVAMLLLSLVAFLVLLSRMVSQHAIPNTDVS
                     VFNLMREIRNAPADELMVPLTMLGDGVVLTAMMVTIVGWLVWRKAWRAAIASTLVFLA
                     GKMFAVLLKYGIHRPRPLELYTGAESFSFPSGHATMAAITFGVLAVLASHAMNRWNRS
                     LVYAICGLIVITIAYSRLYLGVHWLSDILGGVVFGGIMVAAFGVAIEAIPPRRIMPLG
                     LLGATFVAFVAAGALNISNNFERAEKFYAAPEQSYSMVPAEWESFAWRDLPERRIDLA
                     GKPEEQFVLQWAGSLEALQKILEGQGWVYSPKWTWRDSIAYIDPNAVLESVAPRPALH
                     EGLKARLTMIHVLDGQPNRRLVMRAYKTHASLRSAAASTPIFLISLTQESLRKSLRAY
                     VVPTLVAATAAQAADFANDLALPENTRSVIERRPAGGGEVRLITLP"
     gene            3167..>3882
                     /locus_tag="PKV23_03160"
     CDS             3167..>3882
                     /locus_tag="PKV23_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008972684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQX84464.1"
                     /translation="MEDLELNKIQPPPASVGLWLGYGALCVGMFMAILDIQVVVTSLA
                     VIEDALKIGADRMSWVQTTYLIAEIISIPLTGLLIRIFTIRWLFVGAIFTFTLASIGC
                     AFSYDFFSLILFRVLQGFAGGVLIPLVFAAIFLLFGKGLNQTIATTIGGLLAVLAPTL
                     GPLTGGWITESYTWHWLFLINVAPGIVTLVIGIFCLPRNLTRLSQFRTLDWISLAYVS
                     VALAALLVGLKEAPEQGWLAP"
BASE COUNT          853 a         1129 c         1154 g          746 t
ORIGIN      
        1 cagatatcaa ccgccccctg agtataggcg atgacgccgc cttccgtgag cttggtttca
       61 gagccgcccg gttcaagcga aatgaatttg gagcccaaca gcccttcgga cgtcaccttg
      121 gcggaggtgt cgtcggtaag ctgcacggca ggatcaatgc tcatggtcac aagagcctgg
      181 aaattttccg gattgagcga ttgcgcggtc accgtgccga ccttgatgcc ggccatgcgg
      241 atgtcggtgc cgacgttgac gccttcgaca ttgtcgaatt cggcggagat gagataaccg
      301 ccacctttct ggcccatgcc aggagtatta taggcaaaca ccagaaaggc cccggcgatc
      361 gcgatgacca cagccccgat aagtgtttca accgtcgaat tgttcatggc gcttcgcccc
      421 tgattctagg cacattcaag gctttggatg ataacagaaa caactgagac aaagtatggc
      481 gcattgcagc ccgggattaa tccggtcgcc aggcctgata accgggatca gattttttgg
      541 gccgcgccgc gcccacaatg ctgccggggg gacgataggc accggccgag ccggtgagat
      601 tggcctggtg aggcttttgc cactcacggt ccggggcatc ggatgacgat ggcaccttgt
      661 caacccggtg atgcatccag ccgtgccagc ccgggggaat gctcgaagcc tccgaatagc
      721 cattgaaaat cacccagcgg cgttcggcaa tagagtcagg catggccgac ttggcgcggt
      781 aataatggtt gccggcctgg tcggttccca catgttcacc cttgcgccag gtgaaaaaac
      841 gggtggcgag tgtctggcca ctccaccagg taaaaaactg cttcagaaaa tgcatgcttg
      901 gtcctgccaa aggtctcgaa atccgggcgg aatatggccc aatgacggac attcgtaaag
      961 ctggcaaatc gtcctcatgg cagggtgatc aagcgcacct cgcccccacc ggcagggcgc
     1021 ctttcgatga ccgaccgggt gttttcgggc aaggccagat cattcgcaaa atctgcggcc
     1081 tgtgcggccg ttgccgccac cagggtcgga accacgtagg cccgcaggct cttgcgcagg
     1141 ctttcctgag tgaggcttat gaggaagatg ggagttgagg cagcggccga cctgagactg
     1201 gcatgggtct tgtaagcccg catgacaagg cggcgattgg gttggccgtc caggacatgg
     1261 atcatcgtga gcctggcctt gaggccttcg tgaagggcgg gacgcggcgc cacactttcg
     1321 agaaccgcat tgggatcgat ataggctatg ctgtctcgcc aggtccactt tggtgaatac
     1381 acccagccct ggccttcgag gattttctgg agagcctcaa gagagcctgc ccactgcagg
     1441 acaaattgct cttccggttt cccggcaaga tcgatgcggc gctccggcaa atcacgccag
     1501 gcaaaggatt cccattcggc aggcaccatg gaataggatt gttcaggggc ggcgtagaat
     1561 ttttccgccc tttcaaaatt gttcgagata ttcaaggcgc cggcagccac gaaagcaacg
     1621 aaagttgccc cgagcaatcc caaaggcatg atacggcgcg gcgggatggc ttctatggca
     1681 acgccaaagg ccgccaccat gatgcccccg aacacgacgc cgccaaggat atctgacaac
     1741 cagtgaacgc ctagatagag ccgtgaatag gcgatggtga tcacgatcag cccgcaaatg
     1801 gcatagacca gcgaacggtt ccagcggttc atggcatggc ttgccagcac ggccaggacg
     1861 ccaaaagtga tggccgccat ggtggcatgg cccgatggaa aactgaagga ttcagcgccg
     1921 gtataaagct cgagcggccg cggccggtga atgccgtact tgagcagcac ggcaaacatt
     1981 ttccccgcaa ggaacacgag cgttgaggca atggccgccc gccacgcctt gcgccagacc
     2041 agccagccaa caatggtcac catcatggcg gtcaacacca cgccgtcacc cagcatggtc
     2101 aggggaacca tcaattcatc ggcgggggcg ttcctgattt cgcgcatcag gttgaaaacc
     2161 gaaacgtcgg tgttcggaat ggcgtgttgg gacaccatac ggctgagcag cacaaggaag
     2221 gcaacgaggc tcagtagtag catcgcaaca aagaatatga tcagcagcga gcgcggattt
     2281 tccggcgcca cggcgcgccc gaaacggtgc aaggaacggt tgttgcgggt tttcgcccag
     2341 gcggaaaccc gttgcaggat atgatcgagg cggggcgata cgccggcagc gaaaagccgg
     2401 atgagccaac cggccacggc gaggatcacc agcagggcaa ccaacaccag accgaggtgg
     2461 ccgctgagtt cgcccgccag cgccagcccc tgcccgatca atattccggg gaggacatgg
     2521 gcgatggtcc agacgatgcc cgaggaaatg ttgactgtgg caaaatacag ctggctcatg
     2581 cccagcatgc cgacgatgcc gggcacgaca gccttcacgc ccggcacgaa acggcccagg
     2641 gctatgctct tgccgccgtg atttctgaca aactcttcgc cgcgggccac cagttgcgga
     2701 aaccggttca agggccacag gatcttcagg tgttcaccaa agaagcggcc ggcccaatag
     2761 gaaatctggt ctcctgccac agcgccgatg agggttgcca ggaaaaccgg ccagaacggc
     2821 aggtggcccg agcccaccag cactccggcc cccaccagaa cggcggttga aggtacaaac
     2881 aacccaataa tcagaagcgc ttcgccaaag gcgatcagga aaacgacaac aatcgcccac
     2941 tccggattgc gggcaaaata gtcgagaaaa ggctggatgt gatccatatc gtggggctgg
     3001 ggctcgtcgt cgtcctgagg ttagcgccaa atgggggcag gatgtatgtc ctgtctacaa
     3061 ctatttgatt acagcaactt aaaccgcgta aatctgcggg aaagaagggc cgcggcaaac
     3121 agaatcagat gatatcgcgc acggtttgct tctaacatgc ccggcgatgg aagatctgga
     3181 gttgaacaag atccagccgc cgccggcaag cgtcggactc tggctggggt acggggcgct
     3241 gtgtgtcggc atgttcatgg ccattctgga tatccaggtg gtcgtcacat cgctggcggt
     3301 catcgaggac gcgctcaaaa ttggcgccga ccgcatgagc tgggtgcaga cgacctatct
     3361 cattgccgaa atcatctcca ttccgctcac cggattgctc atccgcattt tcaccatccg
     3421 ttggcttttt gtaggcgcca ttttcacctt cacacttgcc tccattggct gcgccttcag
     3481 ctatgacttt ttctcgctca tcctgttccg ggtgctacaa ggctttgccg gcggtgtgct
     3541 cattccgctg gtgtttgccg ccatcttcct gcttttcggc aagggcctca atcaaaccat
     3601 tgcaacgaca atcggcggcc tgctcgcggt gctggcgcct acccttggcc cgctcaccgg
     3661 tggctggatc acggagagct acacctggca ctggctgttc ctgatcaatg tggcgccggg
     3721 catcgtgacc ctggtgatcg gcattttttg cctgccgcgg aatctcaccc ggctttccca
     3781 gttcagaacg ctggactgga tctcgctggc ttatgtctca gtcgccctgg ctgcccttct
     3841 ggttggcctg aaggaagcgc ccgaacaggg ctggctggcg cc
//
LOCUS       DAORVN010000109         3876 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_18624_length_3876_cov_2.853180, whole genome shotgun sequence.
ACCESSION   DAORVN010000109 DAORVN010000000
VERSION     DAORVN010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3876)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3876)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3876
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18624_length_3876_cov_2.853180"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1165)
                     /locus_tag="PKV23_03165"
     CDS             complement(<1..1165)
                     /locus_tag="PKV23_03165"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006336390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HQX84465.1"
                     /translation="MVEAAPLFRLEGVSKSYGGVRALEKANLDIYPGRIHGILGENGA
                     GKSTLIKAIAGVVAPDEGRMFLDGREISFASPTAANQAGIVCIFQELSLIPDLSVADN
                     IVISDPPTRFGMIDRARQRRIAEEALARAGAEDIHPLALVKDLPLSRRQMVEIAKALA
                     RKPRILILDEATSALTAADVTKIFAVLKRLRSEGLALLYISHRMHEIAELADECSVFR
                     NGRKVETYVAGSKTDNEVVELMIGREYKNVFPPKPPLQTNGATPVLQAENLSWTDRLK
                     DISLSVRAGEIVGLGGLDGQGQRDLLLALFGVLRGCSGNVLIDGTPVKINSPTAAKSQ
                     KIGMALIPEDRKTEGLMLPMTVRDNLSFASLHRISDSGIVNRAMAELGPLSEVA"
     gene            complement(1247..2389)
                     /locus_tag="PKV23_03170"
     CDS             complement(1247..2389)
                     /locus_tag="PKV23_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018238258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HQX84466.1"
                     /translation="MKSAPQLRRFVSIGAVLAAGLAFSGVAKAAGPEVVAGPAAEPEC
                     FAPWAADTKFFKFPKKDGPYRIALANGYIANTWRIQMIQTAKAYAAQPDVAAKLKEFK
                     VVSTGEDVPAQISAINNFIDSGYDAIVVNAQNPTAFGPVIKRAKEAGVILVAFDNILD
                     TQDAINVNVDQKGLGVLWANWLIKHLPEGGKILEVKGVAGTSVDTDRHNGIHETFAAS
                     GKKWDVTEVAGKWDDPTAQKVTADAIATNGPFDSITAQGGDTGVVQAMIDAKHAFVPF
                     GGETENGFRKFCAKHSADGLKCSSAGTGPAQVAVAIKTAIAALEGQVVPQSVKLPLAI
                     VEDPNFKEGEDFFPDQSDNFFVGNSFPTCGINFTAQEIMGQSKENQ"
     gene            2589..3617
                     /locus_tag="PKV23_03175"
     CDS             2589..3617
                     /locus_tag="PKV23_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015666010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HQX84467.1"
                     /translation="MALPRNKSRRVTLDDVATRARVSAVTASRVLRKPEMVSADLRAR
                     VDSAVRELAYIPNQLASALASNHTGRIGVIVPSLTNGVFGDYLRALHDVFIPAGFQVL
                     VLNSHYLAGREEKAIATMLGQFPEAMILAGVDQTPQARKSLLQAGIPVVQTMEMTDNP
                     IDINIGLSQREAGYVAARHLFDLGHRHVGQISAPHDARSRQRLEGYLQACAEFQATPM
                     LASTEEPCTVLLGGELLTELLTRWPKVTGLFCGNDNLALGAMFECQRRGIRVPQDLSI
                     MGFNDLEVSACACPSISTIAVPRYEMARRAAEIILEITRGTGKRPKQRRIDLGFKLIA
                     RDSTGRAS"
     gene            complement(3637..>3876)
                     /locus_tag="PKV23_03180"
     CDS             complement(3637..>3876)
                     /locus_tag="PKV23_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018182730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HQX84468.1"
                     /translation="SLIVVGGMLARGGDFLLSGIRELVYQRCLPLATGELKIILAEPK
                     TDSALIGAAHLVLDDVFSPAKVEDFLARFISKRPS"
BASE COUNT          759 a         1129 c         1180 g          808 t
ORIGIN      
        1 gcgcgacttc gctgagcggg ccaagttctg ccatggcgcg attgacgatg ccgctgtccg
       61 agatgcggtg gagcgaggca aaggacaggt tgtcgcgcac tgtcatgggc agcatcaggc
      121 cttcggtctt gcggtcctcg gggatgaggg ccatgccgat tttctggctt ttggccgccg
      181 tcgggctgtt gatcttcact ggcgtgccgt caatcaggac attgccggag cagccgcgca
      241 gcacgccgaa gagcgccaag agaagatcgc gctgcccctg cccgtcgagt ccgccgagac
      301 cgacaatctc gcctgccctc acggacagcg aaatatcctt gagccggtcg gtccaggaca
      361 ggttttcagc ctgcagcacg ggcgttgcgc cattggtttg cagcggcggc ttgggaggga
      421 acacattctt gtattcacgc ccgatcatga gctcgaccac ctcattgtcg gtcttgctgc
      481 cggccacata agtctcgacc ttccggccgt tgcggaacac ggaacactcg tcggcaagct
      541 cggcgatttc gtgcatgcgg tgggaaatat agagaagcgc caggccttcg ctgcgcaggc
      601 gcttgagcac ggcaaaaatc ttggtcacgt cggccgcggt gagcgccgac gtggcctcat
      661 caagaatcag gatgcgcggc ttgcgggcca gcgccttggc gatttccacc atctggcggc
      721 gcgacagggg aaggtccttc acgagggcaa ggggatgaat gtcttcggct ccggcccggg
      781 ccaaagcctc ttcggcgatc cggcgctgcc tggcgcggtc aatcatgccg aagcgcgtgg
      841 gcggatcgct gatcacgata ttgtcggcga ccgaaagatc gggaatgagc gagagttcct
      901 gaaaaatgca gacgatgcct gcctgattgg cggcggttgg cgaggcaaag gagatttccc
      961 tgccatcgag gaacatgcgg ccttcatcgg gcgccacgac gccggcgata gcctttatga
     1021 gcgtggattt gccagcgccg ttctctccca gaatgccgtg gatgcggccg ggatagatat
     1081 caaggttggc cttctcaagg gcacggacgc caccgtagct cttggacacg ccttccagac
     1141 ggaatagtgg agctgcttcg accacgggag tcctccagga tgaccggaca ccaaaagcag
     1201 cgccgcagaa ttgcttccgc gacgccactc gggagtttag atcagattac tggttttctt
     1261 tcgattggcc catgatttcc tgagccgtga aattgattcc acaggtcggg aaagagttgc
     1321 caacgaagaa gttgtcggac tggtcaggga aaaagtcctc gccttccttg aagttcgggt
     1381 cctcgacaat cgccagcggc agcttgacgg actgcggaac gacctggcct tcaagggcag
     1441 caatcgccgt cttgatggca acagcaacct gggccgggcc cgttcctgct gaagagcact
     1501 tcaggccatc agcagaatgc ttcgcacaga acttgcggaa gccgttttcg gtttcaccgc
     1561 cgaaaggcac gaatgcatgc ttggcgtcga tcatcgcctg cacaacgccg gtgtcgccac
     1621 cctgcgcggt aatgctatca aacggaccat tagtggcaat ggcgtcagcc gtcaccttct
     1681 gggcggtagg gtcatcccac ttgcccgcaa cttcggtcac gtcccacttc ttgccggagg
     1741 cggcaaaggt ttcgtgaatg ccattgtggc ggtcggtgtc gacggaggtt ccggcaacgc
     1801 ccttcacttc aaggatcttg ccgccctcag gcagatgctt gatcagccaa ttggcccaga
     1861 gaacgccgag gcctttctgg tccacgttca cgttgatcgc gtcctgggtg tcgagaatgt
     1921 tgtcaaaggc aaccagaatg acgccggctt ccttggcccg cttgatgacg gggccgaaag
     1981 ctgtcgggtt ctgggcgttg acgacgatcg cgtcatagcc ggagtcgatg aagttgttga
     2041 tggccgaaat ttgcgccggc acgtcttcac cggtggatac gaccttgaat tccttcagct
     2101 tggccgcaac atccggctgg gcggcatagg ccttcgcagt ctggatcatt tgaatgcgcc
     2161 aggtgttggc gatgtagcca ttggccagcg caatgcggta tgggccatct tttttcggaa
     2221 atttgaagaa cttggtgtcg gccgcccagg gcgcgaagca ttcaggttct gcggcggggc
     2281 ctgcgaccac ttcgggtccg gcagccttcg ccacgcctga gaaggcgagc cctgccgcca
     2341 aaacggcgcc aatgctcaca aaacggcgta gttgtggtgc tgatttcatt gaattctctc
     2401 ccattggttt tgcacgctct cgctttcggg aacagaaagt tcacttgatc ttctgccgtc
     2461 gtcggacgtg tttttttgtt tgcaccaagc atgattttct atgcttgatg agccgaactt
     2521 attgtaggat gatagcgctg tcaattgccc caggtggcgc tttgacacaa aaagattata
     2581 gttgcgacat ggccctgccg agaaacaaat cccgccgcgt gacactcgat gacgtggcca
     2641 cccgggcgcg ggtgagcgcg gttaccgcgt cacgagtcct gcgcaagcca gagatggttt
     2701 ccgccgatct tcgtgcccgg gttgacagcg cggtgcggga gctcgcctat atccccaacc
     2761 agcttgcaag tgcgcttgcc tccaatcaca caggccggat cggggtcatt gtcccctccc
     2821 tcaccaatgg tgttttcggc gactacctgc gggccttgca tgatgttttc attcccgcag
     2881 gtttccaggt gctggttctc aattcgcatt atcttgcagg ccgtgaggaa aaagccattg
     2941 caaccatgct tgggcagttt ccggaagcga tgatccttgc cggggttgac caaacaccgc
     3001 aggcgcgcaa gtcactgctg caggccggca tccccgtggt gcaaaccatg gagatgacgg
     3061 ataaccccat cgacatcaat atcggcctat cgcaacggga agccggctat gtggcggcgc
     3121 ggcacctgtt cgatcttggc catcggcatg taggccagat ttccgcgccg cacgacgccc
     3181 gttcgcggca acgcctggag ggctaccttc aggcttgcgc cgagttccaa gcaacgccca
     3241 tgctggcttc caccgaggag ccttgcacag ttctcctagg gggcgagctg ctcaccgagc
     3301 tgctgacccg ctggccgaag gtgactggat tattctgcgg caacgacaac ctggctctgg
     3361 gagccatgtt tgagtgccag cgccgcggca tccgcgtgcc gcaggacctc tcaatcatgg
     3421 ggttcaacga ccttgaagtt tcggcttgcg cctgcccttc catcagcacg attgccgtgc
     3481 cgcggtatga gatggcgcgc cgcgcggcag agatcattct cgagattacc cgcggaaccg
     3541 gaaaacgccc gaaacagcgc cggattgatt tgggcttcaa gctgattgcc cgcgacagca
     3601 ccggcagagc aagctgattg gggcacggct ggccgttcag gatgggcgct tggagatgaa
     3661 ccgggccaaa aaatcctcaa cttttgcggg gctgaaaaca tcatccagaa caagatgggc
     3721 ggcgccgatc agtgctgaat cggtttttgg ttcggccaga ataattttca gttcgccggt
     3781 ggcgagcggc aaacaccgct gatagacaag ctcgcggatg ccggagagaa gaaagtcccc
     3841 gccacgcgcc agcatgccgc cgaccacgat caggct
//
LOCUS       DAORVN010000110         3860 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_18744_length_3860_cov_3.796321, whole genome shotgun sequence.
ACCESSION   DAORVN010000110 DAORVN010000000
VERSION     DAORVN010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3860
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18744_length_3860_cov_3.796321"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..568)
                     /locus_tag="PKV23_03185"
     CDS             complement(<1..568)
                     /locus_tag="PKV23_03185"
                     /inference="COORDINATES: protein motif:HMM:TIGR00654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HQX84469.1"
                     /translation="MQLSFHTLDVFTKHRFGGNPLAVVLGADALSTEQMQAITREFNL
                     SETIFVMKPEDPANTAKVRIFFPGGEMAFAGHPTVGCAVLLAEMKYKAGCSFETEIRL
                     EEKAGLVPVKVSRIGDHPRAQFTAPVVPFRADAPLPSVAETARALNLEAEDIGFKAHG
                     LASLEGGPRFFFVPVKSRAALEKSRVLEP"
     gene            complement(651..2303)
                     /locus_tag="PKV23_03190"
     CDS             complement(651..2303)
                     /locus_tag="PKV23_03190"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HQX84470.1"
                     /translation="MNFRTVLLAVFLIISALVPAKAEDGSRVILVLDASGSMWGQIDG
                     TAKMEIAKDVVGRVVGAWKPENELGLVAYGHREKGSCEDIEVLREPGALDAGDYMRAV
                     KALNPKGKTPMTAAVRMAAESLKYTERKASVILVSDGIETCGLDPCAIAEELEKLGVD
                     LTVHTVGFGLDDQGAVAQLKCLAENTGGTYSTAENASELEAALTKTVEAVPAPPPEPV
                     APEFNVLGHVMMAEGVELSQGYESATWEFFDAVDGQKGQWIQTEYGTDIKINLPRTGD
                     FIVRVTDDVATVELPITLESGEVEKLDVSLEAGILRVSGMMDEATPMTDAGAAWELLD
                     SSGKWITTKYGAETAFLANAGVYKVRLALGTAKVEQDVEIAAGKLSEQKIALGAGSIE
                     ASGVFVTGGPAVPDSSAVELRKGEPGVDGKHEWITTTYGTTAQFKVPAGKYLVVFTKD
                     YAEGSAPVEVTAGNSSKVEVSLDGGYLAVTGPAESTIEVFSAEKDISGKRKYLATEYG
                     GAVNKAFEAGAYHVIAKGADGAVLGEKDFEVKAGERTEGTIP"
     gene            complement(2349..3110)
                     /locus_tag="PKV23_03195"
     CDS             complement(2349..3110)
                     /locus_tag="PKV23_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018388565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HQX84471.1"
                     /translation="MMTGDVWFWVLAGLATLFVGSAKGGLPLIGTLAVPLMALQISPV
                     VAAGLLLPIYIISDMYGLWIYRKSFDRRNIAILVPAAAIGIIFGWATASVTNEDVVTI
                     LVGLIGLAYCIDAFLKARRNLPPRSADVPRGIFWGAIAGFTSFVSHSGAPPYQMYVLP
                     QRLEKMVYAGTSTIVFAIINLLKLPPYWFLGQVNLGSLETALLLSPLAIIGAFAGYRL
                     TLILPEKIFFRIVEVALFLVSLKLVYDGVAGLTNG"
     gene            complement(3107..3610)
                     /locus_tag="PKV23_03200"
     CDS             complement(3107..3610)
                     /locus_tag="PKV23_03200"
                     /inference="COORDINATES: protein motif:HMM:NF002696.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-degrading domain-containing protein"
                     /protein_id="HQX84472.1"
                     /translation="MSLESDIERLKFQEQKLRFEKFDEADAWRLGTLMRDAAISRNLP
                     LVIDIRIAGRPLFYTALPGTTPDNAEWVRRKINVVMRLHKCSYRAGREIALSGQALDE
                     TRGVNPIDVAPHGGCFPLHIIGTGVVGSITVSGIPQREDHNFVVSCLSEFLGVDHAQV
                     ALPAETA"
     gene            complement(3624..>3860)
                     /locus_tag="PKV23_03205"
     CDS             complement(3624..>3860)
                     /locus_tag="PKV23_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018074220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HQX84473.1"
                     /translation="LRRGIGDISQRMLTETLRHLQRDGLIYRKVYPTVPPSVEYGLTD
                     LGQSLLVPLDKLVEWSAQNHDVVKAARKEFDKAV"
BASE COUNT          797 a         1256 c         1067 g          740 t
ORIGIN      
        1 ggggctcaag caccctggat ttttccagcg cggcgcggga cttgaccggc acaaagaaaa
       61 agcgcgggcc accttccagc gaagccagac catgcgcctt aaagccaatg tcctcggcct
      121 caagattgag cgcccgtgcc gtctcggcaa cgctgggtaa cggcgcatcc gcgcggaaag
      181 gaacaaccgg tgcggtgaac tgggcccgcg gatgatcgcc gatgcgcgag accttgaccg
      241 gcaccagacc cgctttctct tccaggcgga tctccgtctc gaaactgcag ccggccttgt
      301 atttcatttc tgccagcagc acggcgcagc ccacggtggg atgaccggcg aaggccatct
      361 ccccgccggg aaagaagatc ctcaccttcg ccgtgttggc aggatcctcc ggcttcatca
      421 cgaagatcgt ttccgacagg ttgaactcgc gggtgatcgc ctgcatttgc tccgtagaca
      481 gagcatcggc gccgagcacc accgccagcg gattgccgcc gaagcggtgc ttggtaaaaa
      541 catcaagggt gtggaaggaa agctgcatgg cttgctaaat aatatggccg ccgccagccc
      601 cgcaagccaa acattgtccc gattgtcccg gtttgtcctc tgaaaagacg ctagggaatg
      661 gtcccttcgg tccgttctcc agccttcacc tcaaaatcct tctcgcccag aaccgcgccg
      721 tcggctccct tggcaatcac atggtaggcg cccgcttcga aggccttgtt gaccgcgccg
      781 ccatattccg tcgcgaggta tttccgcttg ccggaaatgt ctttttcggc cgagaagact
      841 tcaatggttg actccgccgg tcccgtgacg gccagatagc cgccatctag gctgacctcg
      901 actttcgatg aatttcccgc cgtgacttca accggcgccg aaccctccgc ataatccttg
      961 gtgaaaacga ccaggtattt tccggccgga accttgaact gggccgtggt gccgtaggtg
     1021 gtggtgatcc actcatgttt tccatcaacg cccggctcgc ccttgcgcaa ctcaaccgcc
     1081 gaactgtccg gcaccgcggg tcctcctgtg acgaacacgc cactggcttc aatcgaaccg
     1141 gctcccagcg caatcttctg ctcggataac ttgcccgccg cgatctccac atcctgctca
     1201 acctttgccg tgcccagcgc aaggcggacc ttgtagacgc cggcattggc gaggaaggcg
     1261 gtttccgccc catacttcgt cgtgatccac ttgcccgaag aatccaacag ctcccaggcc
     1321 gctcccgcat cggtcatggg cgtagcctca tccatcatgc cggaaacccg caggatgccg
     1381 gcttcaaggc taacatcaag cttctcgact tcgccggatt caagcgtgat cggcaattca
     1441 acggtggcca catcatctgt cacccggacg atgaaatcgc cggtacgcgg cagattgatc
     1501 ttgatgtccg tgccatactc tgtctgaatc cactggcctt tttggccgtc caccgcatcg
     1561 aagaattccc aggtggccga ctcatagccc tggctgagtt ccaccccttc cgccatcatc
     1621 acatggccca gcacattgaa ttccggcgca actggctcag gcggcggggc gggcacggcc
     1681 tcaaccgtct tggtcagcgc cgcctcaagc tcgctggcgt tttccgccgt gctgtatgtg
     1741 ccgccggtat tctcggcaag gcacttcaac tgcgcaaccg cgccctgatc atcaagcccg
     1801 aaacccaccg tgtgaaccgt gagatcaact cccagttttt caagctcttc ggcgatggcg
     1861 caagggtcaa gcccgcacgt ctcgatgcca tccgacacca atatgacgct ggccttgcgc
     1921 tcggtatatt tcagcgactc cgccgccatg cgcaccgccg ccgtcatcgg cgtcttgccc
     1981 ttgggattga gcgccttgac ggcacgcatg tagtcgcccg cgtccagcgc ccccggctcg
     2041 cgcagcactt cgatatcctc gcaggaacct ttctcgcggt ggccgtaggc gacaaggccg
     2101 agttcgttct ccggcttcca ggcgccgaca acgcggccca cgacgtcttt ggcgatttcc
     2161 atttttgccg tgccgtcgat ctgcccccac atggagcccg aagcgtcgag caccaggatg
     2221 acccgcgaac cgtcttcggc cttcgccggg accagtgcag aaatgatgag aaagacagca
     2281 agcaaaacgg tgcggaaatt catgtctgcg actcccttgc ccactcagga gccaaggtgg
     2341 cagatcgttc agccatttgt caatccggcc acgccgtcat aaaccagctt cagcgagaca
     2401 aggaagagcg ccacctccac aatgcggaag aaaattttct ccggcaggat gagcgtgagc
     2461 cggtaacccg caaaggcacc aatgatcgcc agcggagaca gcagcagcgc cgtttccagg
     2521 ctgccgagat tgacctgccc caggaaccag tagggcggca gcttgagaag attgatgatc
     2581 gcaaagacaa tggttgaagt tcccgcgtaa accatcttct ccaggcgctg cggcagtaca
     2641 tacatttggt aaggcggcgc gcccgaatgc gaaacaaaac ttgtgaaacc cgcaatcgca
     2701 ccccagaaaa tgccgcgcgg cacatcggcg gatctcggcg gcagattgcg gcgcgctttc
     2761 agaaacgcat cgatgcagta ggcaagtcca atgaggccga ccaggatcgt cacaacatcc
     2821 tcattcgtga ccgaagctgt agcccagccg aatatgatgc cgatggcggc ggccggcacg
     2881 aggatggcga tgttgcgcct gtcgaaagac ttgcggtaga tccacaggcc gtacatgtcg
     2941 ctgatgatat agatgggcag cagcaggccc gccgcaacga cgggcgagat ttgcagggcc
     3001 atgagcggca cagccagcgt gccgatcagc ggcaggccgc ctttggcgct gccgacaaat
     3061 agcgtggcga ggcccgccag aacccagaac cagacgtcac ctgtcatcat gccgtttcgg
     3121 ccggaagcgc aacctgggca tgatcgacgc caaggaactc gctgaggcag ctcacgacaa
     3181 aattatgatc ctcgcgctgc ggaattccgg aaacggtgat gctgccgaca acgcccgtgc
     3241 cgatgatatg caggggaaag cagccgccat ggggcgccac atcgatcggg ttcacgccgc
     3301 gcgtttcatc cagcgcctgc ccgctcagcg cgatttcgcg gcccgcacgg taagagcact
     3361 tgtgaagccg catgaccaca ttgattttgc gccgcaccca ttcggcattg tccggcgtgg
     3421 tgccaggcaa ggctgtgtag aacaagggcc gcccagctat gcggatgtcg atgaccagcg
     3481 gcagattccg cgaaatggcc gcatcgcgca tcagcgtgcc aagtctccag gcatcggctt
     3541 catcgaattt ttcaaagcgc agcttctgtt cctgaaactt cagccgctca atatcgcttt
     3601 caagtgacac gcaattttct cccctagacc gccttgtcga attccttgcg cgccgctttc
     3661 accacgtcgt ggttctgcgc cgaccattca accagcttgt ccagcggcac cagaagcgac
     3721 tggcccaaat ccgtcaagcc atattccacg cttggcggca cggtgggata gactttccgg
     3781 tatatcagcc cgtcgcgctg caaatggcgc agcgtctcgg tgagcatgcg ctgcgagata
     3841 tcaccgatcc cgcggcgcaa
//
LOCUS       DAORVN010000111         3842 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_18902_length_3842_cov_3.706364, whole genome shotgun sequence.
ACCESSION   DAORVN010000111 DAORVN010000000
VERSION     DAORVN010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3842
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18902_length_3842_cov_3.706364"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..727)
                     /locus_tag="PKV23_03210"
     CDS             complement(<1..727)
                     /locus_tag="PKV23_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012029700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HQX84474.1"
                     /translation="MSTLIRGGTVVNHDHSVRADLLIDGGKIVGIGEKLSGTTTIDAG
                     GCYVMPGGIDPHTHMELMFMGNVSADDFEWGTKAALSGGTTMIVDFCIPAPGESMLAA
                     YQAWRHKSEKAATDYSFHMAVTSWSPQVHDEMETVVKTYGINTFKHFMAYKGALMVND
                     DELYNSFKRCAQLGALPLVHAENGDVVARMQADLLARGITGPEGHSYSRPPEVEGEAT
                     NRAIMIADMAGSPLYVVHTSCEQT"
     gene            complement(874..1866)
                     /locus_tag="PKV23_03215"
     CDS             complement(874..1866)
                     /locus_tag="PKV23_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009760161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HQX84475.1"
                     /translation="MRKYLLAAAAVGALLASSGGAFAEAVKLQLQWVTQAQFAGYYVA
                     QAKGYYKDAGLEVTIMPGGPDINPQQVLAGGGADVVIDWMPSALATREKGSPVVNIAQ
                     PFKKSGMMLTCRKDTGITKPEDFKGKTLGVWFFGNEYPFLSWMSQLGLPTAGGADGVT
                     VLKQGFNVDPLLQKQADCISTMTYNEYYQLIEAGMKAEELVVFKYEDQGVATLEDGLY
                     VLEDKLKDPAFVTTMAKFVKASMKGWDEARANPAEAVKLVLENDASGSQTEGHQTTMI
                     EEINKLTEGSDGTLDVAAAERTVDTLMKGGSDPVITKKPEGAWTSVVTDAAKAM"
     gene            complement(1901..2671)
                     /locus_tag="PKV23_03220"
     CDS             complement(1901..2671)
                     /locus_tag="PKV23_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018326761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQX84476.1"
                     /translation="MRSSKFNDVIAPALFGIAILILWQATVRYFNVPFVIFPAPTDIW
                     SKLIVSLPILYADFVQTFIRAVIPGWIIGSLSGFLVALLCDRFAFLRQGLLPLGNLAA
                     ALPIVGVAPILVMWFGFDWQSKAAMVVVMTFFPMLVNAVAGLQASGHLERDLMRSYGA
                     SWFQTLVKLRLPAAMPFIFNALKINSTLAMIGAIVAEFFGTPIVGMGFRISTEVGRMG
                     LDMVWAEIAVAALVGSAFYGLLVALERWVTFWHPSYRR"
     gene            complement(2668..3543)
                     /locus_tag="PKV23_03225"
     CDS             complement(2668..3543)
                     /locus_tag="PKV23_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010969951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQX84477.1"
                     /translation="MKTEGRKSSVVPVTVVCVIILVAWYIAAIFMNAVVAEPKIEALG
                     GGFANTVNVSWSLDRPVIPAPHQVVVEVWKTVFTIAPWKVRSLLYHVWVTLSSTLLGF
                     VLGTLLGIGLAVAIVHSRLLSKSLMPWIIASQTIPILAIAPMVIVVLGSIGLTGLVPK
                     SIISMYLCFFPVTIGMVKGLTSPDPMQLDLMRTYNASVSQVLWKLRFPAAMPFLFASL
                     KVAVAIALTGAIVGELPTGAQSGIGSRMLAGSYYGQTIQIWSALVAASAVAAALVYGV
                     GGIERIVNRQMGARA"
     gene            complement(3533..>3842)
                     /locus_tag="PKV23_03230"
     CDS             complement(3533..>3842)
                     /locus_tag="PKV23_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008196156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQX84478.1"
                     /translation="LLLMDEPFGSLDEFVRDKLNQQLLELWAATKKTVVFVTHSIPEA
                     VFLSSKIVVMSPRPGRIIDVIETNLPANRTLDIRETPEFLKIAHRVREGLKAGHSYED
                     "
BASE COUNT          814 a         1204 c         1103 g          721 t
ORIGIN      
        1 gggtctgctc gcaggaggtg tgaacgacat agagcggcga gcccgccatg tcggcaatca
       61 tgatggcccg gtttgtcgct tcgccttcca cttcgggagg ccgtgaatag ctgtggccct
      121 ccggcccggt aatgccacgc gccagcagat ccgcctgcat gcgggccacc acatcgccat
      181 tctccgcatg aaccaagggc aaagcgccaa gctgggcgca gcgcttgaaa ctattataga
      241 gctcgtcgtc atttaccatc aacgcgccct tgtaggccat gaaatgcttg aaggtgttga
      301 tgccgtaggt cttgaccacg gtttccatct cgtcgtggac ctgcgggctc caggaggtga
      361 ccgccatgtg gaaggaataa tccgtggcgg ccttttccga tttgtgccgc caggcctgat
      421 aggcggccag catcgattca ccgggtgcgg gaatgcagaa atccacaatc atcgtcgtgc
      481 cgccggaaag tgccgccttg gtgccccatt cgaaatcatc agccgagaca ttgcccatga
      541 acatgagttc catgtgcgtg tgaggatcga tgccgcccgg catcacatag cagccgccgg
      601 catcaatcgt cgtggtgccg gaaagcttct cgccaatacc cacgatcttg ccgccatcaa
      661 taagcaagtc ggcgcggacc gaatgatcgt gattaacaac agtgccgccg cgaatgagtg
      721 tagacatggg gattctccga actgactggt gcttcgaggc tcatccgggc tcacgcccga
      781 atgagcacct cagcatgagg attggtggaa gtccctcacc ctgaggtgca tggcgcgagc
      841 catgcctcga agggcgaggg gcatcccatt tgcttacatc gccttggcgg cgtcggtcac
      901 gacgctggtc caggcgcctt ccggcttctt ggtgatcacc ggatcggaac cgcccttcat
      961 cagcgtgtcc accgtgcgtt cggcggcggc cacgtcaagc gtgccgtcgg aaccttcggt
     1021 gagcttgttg atctcctcga tcatggtggt ctggtggcct tcggtctgcg agcctgatgc
     1081 atcattctcg agaacgagct tcaccgcctc ggccggattg gcgcgcgcct catcccagcc
     1141 cttcatcgaa gccttgacga acttcgccat ggtggtgacg aaggccggat ccttcagctt
     1201 gtcttccaga acgtaaagcc cgtcttccag cgtggcaacg ccctggtctt catacttgaa
     1261 caccaccagc tcttccgcct tcatgccggc ttcgatgagc tggtaatact cgttgtaggt
     1321 catcgtcgaa atgcagtccg cctgcttctg cagcaggggg tccacgttga aaccctgctt
     1381 cagcacggtg acgccatcgg cgccgcccgc cgtcggcagg ccaagctggc tcatccatga
     1441 caagaaggga tattcattgc cgaagaacca gacgcccagc gtcttgccct tgaagtcttc
     1501 cggcttggtg atgccggtgt ccttgcggca ggtcagcatc atgcccgact tcttgaacgg
     1561 ctgggcgatg ttgacaaccg gcgaaccctt ttcgcgcgtc gccagggccg atggcatcca
     1621 gtcgatcacc acgtcagccc cgccgccggc cagaacctgc tgcggattga tgtccgggcc
     1681 gcccggcatg atggtgactt cgaggcctgc gtccttgtag tagcccttgg cctgggccac
     1741 gtagtagccg gcgaactgcg cctgcgtcac ccactgcaat tgtagcttca cggcttcggc
     1801 aaaagccccg cctgaactcg ccagaagtgc tcccaccgct gctgcggcaa gtaagtattt
     1861 cctcatttcc ctgttctcct tcttctttcc aaattgccgc ttacctccgg taagacggat
     1921 gccaaaaagt tacccagcgc tcgagggcga caagcaaccc gtagaatgcc gaacccacca
     1981 gtgctgccac ggcgatttcg gcccaaacca tatccagccc catgcggccc acttccgtcg
     2041 aaatgcgaaa gcccatgccg acaatcggcg ttccgaaaaa ttccgcaaca attgcgccaa
     2101 tcatcgccaa tgtcgagttg atcttgagcg cgttgaaaat gaaaggcatg gcggcgggaa
     2161 gtcgcagttt caccagcgtc tggaaccagc tggcgccata gcttcgcatc aggtcgcgct
     2221 ccaggtggcc cgaggcctgc aaccccgcca cggcgttcac cagcatggga aagaaggtca
     2281 tgaccaccac catggcggcc ttggactgcc agtcaaaacc gaaccacatg accaggatgg
     2341 gggctacgcc gacgatggga agggctgctg ccagattgcc cagcggcaag agcccctggc
     2401 gcaggaaggc gaaacggtca cagagcagcg ccacgaggaa tcccgacagc gacccgatga
     2461 tccagccggg gatcaccgcg cggatgaagg tctggacaaa atccgcataa agaatgggca
     2521 gcgatacaat gagctttgac cagatgtcgg tgggcgcggg aaaaatcacg aagggcacgt
     2581 tgaagtagcg caccgtggcc tgccacagga tgagaatggc gatgccgaaa agcgccgggg
     2641 cgataacatc attgaacttg gatgacctca tgcccgcgcg cccatctgcc ggttgacgat
     2701 gcgctcgatt ccgccgacgc cataaaccag cgccgcggcc accgccgaag cggccaccag
     2761 cgccgaccag atttgaatgg tctgcccgta ataggaaccg gccagcatgc gcgaaccgat
     2821 gccggattgc gcgcccgtgg gcagttcacc gactatggcg ccggtcagcg caatggcaac
     2881 cgccaccttc agcgacgcaa acaggaaggg catcgcggcg ggaaagcgca gcttccacag
     2941 gacttgagaa acactcgcgt tgtaagtgcg catgagatca agctgcattg ggtccggcga
     3001 ggtgagcccc ttcaccatgc cgatggtgac ggggaagaaa cacagataca tcgaaatgat
     3061 ggacttaggg acaagcccgg tgaggccaat cgaccccagc accacgatga ccatgggtgc
     3121 tattgcgaga atgggaattg tctgcgaggc gatgatccag ggcatcaggc tcttgctcag
     3181 cagcctggaa tgcacgatgg ccactgcaag gccgatgccg agaagcgttc ccagcacaaa
     3241 gccgagcagc gtcgaggaca gcgtcaccca cacatgatag agcagcgacc ttaccttcca
     3301 cggcgcgatg gtaaacacgg tcttccagac ttcgactacc acctggtgcg gcgccgggat
     3361 caccgggcgg tcaaggctcc acgacacgtt caccgtattg gcaaagccgc cgccgagcgc
     3421 ctcgatcttc ggctccgcca ccaccgcgtt catgaaaatc gccgcgatat accaggccac
     3481 gagaatgatc acgcacacca ccgtgaccgg aacgactgag cttttgcggc cctcagtctt
     3541 catagctatg ccccgccttc aggccctcac gcacccggtg ggcgattttc aggaactccg
     3601 gtgtctcgcg gatatccagc gtgcggtttg ccggcaggtt tgtttcgatc acgtcaatga
     3661 tgcggccggg cctgggactc atcaccacaa tcttggacga caggaacacc gcttccggaa
     3721 tcgaatgcgt cacaaacacc acggtttttt tcgtcgccgc ccagagctcc agcaattgct
     3781 ggttcagctt gtcgcgcacg aattcatcga gcgacccgaa aggctcgtcc atcagcagca
     3841 gc
//
LOCUS       DAORVN010000112         3838 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_18938_length_3838_cov_3.461803, whole genome shotgun sequence.
ACCESSION   DAORVN010000112 DAORVN010000000
VERSION     DAORVN010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3838
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18938_length_3838_cov_3.461803"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1270
                     /locus_tag="PKV23_03235"
     CDS             <1..1270
                     /locus_tag="PKV23_03235"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HQX84479.1"
                     /translation="ISVEVKYANAADREGEAAPRQQQFEEFFNQFPQFRNQLPPGFEN
                     RRPRGGAAVGSGFLISADGFAVTNNHVVQDADEVTVKIHGGAELKAEVIGTDPKTDLA
                     LIKVKSDKPFQFVKFAEAPARVGDIVMAVGNPFGLGGSVTTGIVSARGRDIGSGPYDD
                     FLQIDAAINRGNSGGPAFNLQGEVIGVNTAIFSPSGGSVGIGFAIPAEITQDVIASLK
                     ESGSVTRGWLGVQIQPVTDEIAESLGLASAKGAIVSDVTEGSPALAAGFKPGDTILKA
                     DGTEISDARDLSRKVARFAPGKSVNVDIVRDGKPMSVTVKIGTMPNDVRMARRGGNPD
                     SADVASLGMKLGPAEDGAGVKIVEVAPNSIAAEQGLKPGDIILEVAGTDVNGPADVRD
                     ALKAAGKSRVLMLVRSGDSQRFLALPRAKG"
     gene            1456..2130
                     /locus_tag="PKV23_03240"
     CDS             1456..2130
                     /locus_tag="PKV23_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HQX84480.1"
                     /translation="MRILVIEDDRDAASWLIKGLTESGHVTDHAANGEDGLAMASENL
                     HDILIVDRMLPKMDGLSIIRALRAKGVTTPVLILSALSDVDERVKGLRAGGDDYLAKP
                     YAFSELLARVEGLGRRNSQEPLETKLRAGDLEIDLLTRAVTRAGQTIPLQPREFKLLE
                     YLMRNAGHIVTRTMLLENVWDYHFDPQTNVIDVHVSRLRSKIDKGFDEPLLQTVRGAG
                     YMIRAV"
     gene            2120..3511
                     /locus_tag="PKV23_03245"
     CDS             2120..3511
                     /locus_tag="PKV23_03245"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HQX84481.1"
                     /translation="MQFNPKILKTSTFRLAAIYLLVFALSVGSILAYVFWNTAGLLER
                     QTDATIRAEVQALADQYRLLGLRGIVDTVQRRSAERGGGVYLIADSLGRRVAGNLESV
                     PPQVIDETGWIDFPLDIQIGPNKQRRSARAFHTDLKDNYELIVGRDVQELRQFGDIIR
                     RTLYWALGIALVLGLGGGLLTSRNFLRRVDAITDASRAIMGGNLSSRMPVKGTGDELD
                     KLSRSLNEMLDQIERLMTGMKEVSSNVAHDLKTPLTRLRARVESALRSNSKTEYRAAL
                     DKTIEESDRLLQTFNALLSIARAEAGQNKEMLQAIDAREIVEGVAELYEPIAEEDGGT
                     LHVKAAAGLNVLADRQLLSQALSNLVDNALKYGVTGENPKPHIEIEGKLEEGNVVILV
                     ADKGAGIAAEDRTRVLDRFVRLDESRSKPGNGLGLSLVAGVMKLHGGTLLLEDNTPGL
                     RAKLVLPLHQSQS"
     gene            3521..>3838
                     /locus_tag="PKV23_03250"
     CDS             3521..>3838
                     /locus_tag="PKV23_03250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX84482.1"
                     /translation="MTGVFSERLKQHLPAPRDMEAAARFWNDLTPGHQAMLQDHRAFL
                     EAVAGGSPYLRTLMLRDPQFLADLFHHAPEDELALLCAGLHTAQSLLTQAEIMTALRH
                     AKAK"
BASE COUNT          761 a         1247 c         1117 g          713 t
ORIGIN      
        1 gatcagcgtt gaagtgaaat atgccaatgc cgccgaccgg gaaggcgaag ccgccccgcg
       61 ccagcagcag tttgaagagt tcttcaatca gtttccccag ttccgcaacc agttgcctcc
      121 gggctttgaa aaccgccggc cccgcggcgg tgccgccgtg ggctcgggct tcctgatcag
      181 cgccgatggc tttgccgtaa ccaacaacca cgtggtccag gacgccgatg aagtgaccgt
      241 caagattcac ggcggcgcgg aactcaaagc cgaagtgatc ggcaccgatc ccaagacgga
      301 tcttgccctc atcaaggtca agtcggacaa gccttttcaa ttcgtgaagt ttgctgaagc
      361 gcccgcccgt gtcggcgata tcgtgatggc cgtgggcaat ccctttggcc ttggcggctc
      421 ggtgaccacc ggcatcgttt cggcccgtgg tcgcgacatc ggatccggcc cctatgacga
      481 cttcctgcag attgatgccg cgatcaaccg cggcaattcc ggcggcccgg cctttaacct
      541 gcaaggtgag gtcatcggcg tgaacaccgc gatcttctcg ccttcgggcg gctccgtcgg
      601 catcggcttt gcgattccgg cagaaatcac ccaggacgtg attgccagcc tcaaggaaag
      661 cggcagcgtg acccgcggct ggctcggcgt gcaaatccag ccggtgaccg atgagattgc
      721 cgaaagcctg ggccttgcca gcgccaaggg tgcgattgtc tcggatgtca ccgaaggctc
      781 acccgccctt gccgcgggct tcaagccggg cgacaccatt ctcaaggccg atggcacgga
      841 gatatccgat gcccgcgacc tgtcgcgcaa agtcgcccgc ttcgctccgg gcaaatccgt
      901 caatgtggat atcgtccgtg acggaaagcc catgtcggtt accgtcaaga tcggcaccat
      961 gccgaatgac gtgcgcatgg cccgccgcgg aggcaacccg gattcggctg atgtcgcctc
     1021 gcttggcatg aaactgggcc cggccgagga tggcgccggc gtgaagattg tcgaagttgc
     1081 gccgaattcc attgccgcgg agcagggcct taagcctggc gacatcattc ttgaagttgc
     1141 cggcaccgac gtcaacggcc cggccgatgt tcgggacgcc ttgaaggccg ccggcaaatc
     1201 ccgcgtcttg atgctggtgc gctccggcga cagccagcgc ttcctggcgc tgccacgcgc
     1261 caagggctaa cggttaggac gcccgaatgc cccctccgct cggcatttga cgtccaatag
     1321 cgcggaggcc gccccctcca aaggttcccc cctggtccgg tggcctccgc gcactctttt
     1381 ctgaaagggc aagtcatgca agtctccacc tcacacaaga ccgaatctca aggattggga
     1441 ctggccaccg aaagaatgcg cattctcgtt attgaagatg accgcgatgc ggcttcctgg
     1501 ctgatcaagg gactgaccga aagcggccac gtcaccgacc acgccgccaa cggcgaggat
     1561 ggcctggcca tggcgtcgga gaatttgcac gacattctca ttgtcgaccg catgctgccc
     1621 aaaatggacg gcctttcgat catccgcgcc cttcgcgcca agggggtcac cactcccgtc
     1681 ctcatcctga gcgccctgag tgacgtggac gagcgggtca aaggcttgcg ggccggcggc
     1741 gatgattacc tggcaaaacc ttacgccttt tccgagcttc tggcccgcgt tgagggcctg
     1801 ggccgccgca attcccagga gccgctggaa acaaaactgc gggctggcga tctggagatc
     1861 gacctgctga cccgcgccgt aacccgcgcc ggccagacca ttccgctcca gccccgcgag
     1921 ttcaagctgc tcgaatatct catgcgcaac gccgggcaca tcgtcacccg caccatgctg
     1981 ctggagaatg tctgggacta tcatttcgat ccgcagacca atgtgataga cgtacacgta
     2041 tcaaggctcc ggtcgaagat cgacaagggc tttgatgaac cccttctgca aaccgtgcgc
     2101 ggagccggat acatgatacg tgcagtttaa tccgaagata ctgaagacct ccacctttcg
     2161 tcttgcggcc atctacctgc tggtctttgc actgtccgtg ggctccatcc tggcctacgt
     2221 tttctggaac acggcgggcc ttctggaacg ccagaccgat gccacgatcc gcgccgaggt
     2281 ccaggctctg gccgatcagt accggcttct tggcctgcgc ggcattgttg acacggtcca
     2341 gcgccgcagc gccgagcgtg gcggcggcgt ctatctcatc gccgattcct tgggaaggcg
     2401 cgtggccggc aatcttgagt ccgtgccgcc ccaggttatc gacgaaaccg gctggattga
     2461 ttttcccctc gatatccaga ttggcccaaa caagcagcgc cgctcggccc gcgcctttca
     2521 caccgacctc aaggacaatt atgaattgat cgtcggccgc gatgttcagg aactgcggca
     2581 attcggcgac atcatccggc gaaccctcta ctgggccctg ggaattgccc ttgttctggg
     2641 tctaggcggc ggcctgctca ccagtcgcaa cttccttcgc cgggttgacg ccatcactga
     2701 cgccagccgc gccatcatgg gcggcaacct ttcaagccgc atgccggtca agggaaccgg
     2761 cgatgaactg gacaagctgt cccgttcgct caacgaaatg ctcgaccaga tcgagcgcct
     2821 gatgaccggc atgaaggaag tcagcagcaa tgtcgcccat gacctgaaaa ccccgctcac
     2881 ccggctgcgg gcccgcgttg aatccgccct ccgttccaac agcaaaaccg aatatcgcgc
     2941 cgccctcgac aagaccatcg aggaatcgga ccgcctgctg caaaccttca atgcgcttct
     3001 ctccatcgcc cgcgctgaag ccggccaaaa caaggaaatg cttcaggcca tcgatgcccg
     3061 cgagattgtc gaaggggttg ccgaactcta cgaacccatt gccgaggaag atggcggaac
     3121 cctgcatgtg aaagcggctg ccggcttgaa tgtattggcc gaccgccagc tcttgtccca
     3181 ggccctgagc aatctggtgg acaatgccct gaaatacggc gtgaccgggg aaaatcccaa
     3241 accgcacatc gaaattgaag gaaagctgga ggagggcaat gtcgtcatcc tcgtggccga
     3301 caagggcgcg ggcatagcag ccgaagaccg gacccgggtg ctggatcgct ttgtgcgcct
     3361 cgacgaaagc cgcagcaagc ccggaaacgg cctgggcctg agcctcgtgg ccggcgtcat
     3421 gaaactccac ggcggcaccc tgctgctcga agacaacacg ccaggcctcc gcgccaagct
     3481 ggtgcttccg cttcaccaga gccaaagcta gtatggcccc atgactgggg ttttttctga
     3541 acgcttgaag cagcacttgc ctgcaccgcg agatatggaa gctgctgcaa ggttctggaa
     3601 tgacttgacg cctggccatc aggcgatgct gcaagaccac cgggcatttc tcgaagccgt
     3661 ggctggcggc tcgccttact tgcgcaccct catgctgcgc gacccgcaat ttctggcaga
     3721 cctgtttcac catgcgccgg aagatgagct ggcgttgctc tgcgccggcc tccacaccgc
     3781 gcaatcgctc ctgacccagg ctgagattat gacggcgctg cgtcatgcca aggcaaag
//
LOCUS       DAORVN010000113         3836 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_18967_length_3836_cov_2.894208, whole genome shotgun sequence.
ACCESSION   DAORVN010000113 DAORVN010000000
VERSION     DAORVN010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3836
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18967_length_3836_cov_2.894208"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..590)
                     /locus_tag="PKV23_03255"
     CDS             complement(<1..590)
                     /locus_tag="PKV23_03255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX84483.1"
                     /translation="MDQSSTNDTDKRNWRERLGIGGKDLPRISGEFSKTPETVVQESE
                     PVRAPQPVAKPAPMAPRIPMKAAPAPAPRPQQQVMPQGAAPQAPDALAEKLRAQRAAA
                     ERLAEQRVTAAKQRAESAASTPAPAPAPVSAAAKAPIIAGGPAEKPKFSFADEDSKPD
                     FGREARTLPPGRPPQRPAPQQPQLSPPRPPLGGQPR"
     gene            complement(609..2342)
                     /gene="argS"
                     /locus_tag="PKV23_03260"
     CDS             complement(609..2342)
                     /gene="argS"
                     /locus_tag="PKV23_03260"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019965725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HQX84484.1"
                     /translation="MNLFGHFQTLVRNELSAMMAAGELKPGLDISRVTAEPPRDASHG
                     DVTTNAAMVVAKSAGMAPKVLAETLAKRLAKQPDVLSAEVAGPGFINLRLKETFWPKI
                     LNAILELGDTYGHSELGKGIRTNVEYVSANPTGPLHVGHCRGAVFGDALAALLRVTGY
                     DVSTEYYINDAGAQVDTVARSAFLRYQEALGTDIGEIPAGLYPGEYLIPVGQALAAEH
                     GKNLLSMKEEQWLPIARTKAIAMMMDLIRGDLASLKIHQDIFFSERSLHESLEVRDTV
                     ADLRAKGHVYEGRLPPPKGELPEDWEDREQTLFRATAFGDDMDRPLLKSDGSYTYFAS
                     DVAYSRNKIRRGFKELIYVLGADHGGYVKRLEAVAAAIAGPGQVDVIVRLCQLVKLFR
                     NGEPVRMSKRSGEFVTLRDVVDEVGPDVVRFMMLYRKNEAPLDFDFARVTEQSKDNAV
                     FYVQYAHARVCSVLRNGADVGQTGINHDFSLLIDEAERHVVKRLAEYPKMLEGAALAH
                     EPHRISFYLYELASDFHSLWNKGKESPQLRFIVKNELILTDTRLAFLSAIRYVLGNGL
                     RILGVDPVEEM"
     gene            complement(2438..3634)
                     /locus_tag="PKV23_03265"
     CDS             complement(2438..3634)
                     /locus_tag="PKV23_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HQX84485.1"
                     /translation="MKRHTASYAARPEESRGRLFPEAESPPRSAFERDRDRVIHSSAF
                     RRLKHKTQVFVYHEGDHYRTRLTHSIEVAQVARAIARVMGLDEDLTEALALAHDLGHT
                     PFGHAGERELDKLMSPYGGFDHNAQSLRIVTKLERKYAKFDGLNLTWETLEGLVKHNG
                     PLIDSRGHALGAFKDSGLPQVIIEYAEAHDLKLASFASVEAQVAAIADDIAYNAHDID
                     DGLRAKLFDIIDLGDVPLVGEALSGVLKLYPGLERSRIIHETVRRVISAMVTDVVAQT
                     KKNCADAGVKSSDDVRAQKRPVVQFAPQMAENNRVLQAFLSTRMYRHARVLEIMERAQ
                     RVIRDLFEAYMNDASLLPEDWREENIQGDRSHYARQVCDFLSGMTDRFALEQHKHLFD
                     LDPLFR"
BASE COUNT          743 a         1115 c         1179 g          799 t
ORIGIN      
        1 atgcggggct ggccaccgag cggcgggcgc ggcggggaca gctgcggctg ctgcggcgcg
       61 ggcctttgcg gcggacggcc gggcggcaaa gtgcgcgcct cgcggccaaa gtcaggtttt
      121 gaatcctcat cggcgaaact gaatttcggc ttttcggccg ggccgccggc gatgatcggc
      181 gccttggccg cggcggagac cggcgctggc gccggtgctg gtgtgctcgc ggcgctttca
      241 gcccgttgct tggccgccgt tacccgctgt tcggccagcc tctcggcggc ggcgcgctgg
      301 gcgcgcagct tctcggccag ggcatcaggg gcctggggag cggcgccctg cggcatgacc
      361 tgctgctggg gcctgggtgc gggtgccgga gctgccttca tgggaatgcg cggcgccatg
      421 ggtgctggct ttgccacagg ctggggagcc cggacaggct ctgactcctg aacgacggtc
      481 tcgggcgtct tgctgaactc ccctgaaatg cggggcaagt ccttgccacc aatacctaaa
      541 cgctcgcgcc agttgcgctt atcggtgtca ttcgtggagc tttgatccat tacccgtcat
      601 ttccgttgtt acatttcttc gacagggtct acccccagga tgcgaagacc gttccccaaa
      661 acatagcgaa tcgccgacaa gaaagcgagg cgcgtatcgg ttaatattaa ctcatttttt
      721 acaataaatc ttaattgcgg cgactctttg cctttgttcc agagggaatg aaagtcactg
      781 gcgagctcat agagataaaa cgagatgcgg tgcggctcgt gggcaagtgc tgcgccctcc
      841 aacatttttg gatattctgc cagcctcttt accacatggc gctcggcttc gtcgatcaat
      901 aatgaaaaat catggttaat tcccgtctgg ccgacgtcgg ctccgttgcg caacaccgag
      961 cagacgcggg cgtgggcgta ctgcacgtag aatacggcat tgtccttcga ctgctcggtg
     1021 accctggcaa aatcaaaatc cagcggcgcc tcgttcttgc ggtagagcat catgaagcgg
     1081 acaacgtcgg ggccaacctc atcgacgaca tcgcgcaggg tgacaaactc accggaacgc
     1141 tttgacatgc gcacgggctc gccgttgcgg aacagtttca ccaactggca gagcctgacg
     1201 atgacatcga cctggccagg tccggcaatg gccgcggcca cggcttcaag tcttttgaca
     1261 tagccgccgt ggtcggcgcc gagaacatag atcagttcct tgaagccgcg cctgatcttg
     1321 ttgcggctgt aggcaacgtc ggaggcgaaa taggtgtagg agccgtcgga cttaagcaag
     1381 ggccggtcca tgtcatcgcc aaacgccgtg gcgcggaaaa gggtttgctc gcggtcttcc
     1441 cagtcttccg gcaattcgcc cttgggcggc ggcaggcggc cttcatagac atggcccttg
     1501 gcgcgcagat cggccaccgt gtcacgcact tcaagcgact catgtaggga cctttctgaa
     1561 aagaaaatgt cctggtgaat cttcaagctg gccagatcac cgcggatcaa gtccatcatc
     1621 atggcgatcg ctttggtccg ggcaatggga agccactgct cctccttcat cgagaggaga
     1681 ttttttccat gctcggcggc aagcgcctgc cctaccggga tcaggtattc accgggataa
     1741 aggccggctg gaatttcgcc aatgtccgtg cccagagctt cctgatagcg caggaaggcg
     1801 gagcgcgcga ccgtatcaac ctgggcgccg gcatcgttga tgtagtattc ggtgctgacg
     1861 tcatagccgg tcaccctgag caaggcagcc agggcatcgc cgaacaccgc gccacggcag
     1921 tggccgacat gcaagggccc ggtcggattg gccgagacgt attctacatt tgtcctgatg
     1981 cccttgccca attcggagtg gccgtaggta tcgccgagct ccagaatggc gttgaggatc
     2041 ttgggccaga aggtttcctt gaggcggaga ttgatgaagc cggggcccgc gacttcggca
     2101 gataaaacat ccggctgttt tgccaggcgc ttcgcaagag tttcggccaa caccttcggc
     2161 gccatgccgg cggactttgc caccaccatg gcggcgttgg tcgtcacatc gccatgtgaa
     2221 gcatcacgcg ggggttccgc cgtgacgcgc gaaatatcta ggccgggctt cagttcaccg
     2281 gctgccatca tggctgaaag ctcgttcctg acgagcgtct gaaagtgtcc aaaaaggttc
     2341 attttatcat tctaagttat tgaaattgta taatttgcta ttttagcttg gcgcgtgatc
     2401 ggacggaaaa ccggtgccca cttttcctga tcacgcgcta cctgaaaagc gggtcgaggt
     2461 caaacaagtg cttgtgctgc tccagggcaa agcgatcggt catgccggaa agaaagtcgc
     2521 agacctggcg ggcatagtgg ctgcggtcac cctggatatt ttcctcacgc cagtcctcgg
     2581 gcaagagcga ggcatcattc atgtaggctt cgaacaagtc acggatcacg cgctgggcgc
     2641 gctccatgat ttcaaggaca cgggcgtggc ggtacatcct tgtcgacagg aaggcctgca
     2701 gcacccggtt gttttccgcc atctgggggg cgaactgcac cacgggccgc ttctgggcgc
     2761 gcacgtcgtc gcttgatttc acgccggcgt cggcgcagtt ttttttcgtc tgggcgacga
     2821 catcggtcac catcgccgaa ataacgcggc gcaccgtctc gtgaatgatg cgtgaccttt
     2881 ccaggccggg gtaaagtttc aggacaccgg acagggcctc ccccacgagc ggcacatcgc
     2941 cgagatcgat gatgtcaaag agcttggcgc gcagcccgtc atcgatgtca tgggcattgt
     3001 aggcgatatc atcggcgatg gcggcgacct gcgcttcaac ggaggcaaag cttgcaagct
     3061 tgagatcatg ggcctcggcg tattcaatga tgacctgcgg caagccgctg tccttgaaag
     3121 ccccgagcgc atggccgcgc gaatcaatga gcgggccatt gtgcttgacc aggccctcaa
     3181 gagtttccca ggtaagattc aagccatcga atttcgcgta tttccgctca agcttcgtta
     3241 cgattcgcag ggactgggca ttgtggtcaa agccgccata aggtgacatg agtttgtcga
     3301 gctcgcgctc gccggcatgg ccgaaggggg tgtggccaag gtcgtgggcc aaggccaggg
     3361 cttcggtcag atcttcatcg aggcccatga cacgggcgat ggcccttgcc acctgggcca
     3421 cctcaatgga atgggtaagc cgggtgcggt agtggtcgcc ctcatgatac acaaaaacct
     3481 gggttttatg cttcaggcgg cggaaggctg aggaatggat gacgcggtcc cggtcccgct
     3541 cgaaggccga gcgcggcggg ctttcagcct ccgggaacag ccgtcccctg ctctcctccg
     3601 gccttgccgc atagcttgcc gtatgacgtt tcaccttacc gcaccattga caatcagctg
     3661 cccaggaccg acataaatgc ccatggaaag cactgtaaac attactgccc gcgccgcaaa
     3721 ccgtatttgt gaaatcaccg ccggccagac tgagctgcgc ggcctgcgga tttcggtgaa
     3781 tggcggcggc tgttcgggct ttcaatatga ctttgccatc gccacggacc gcaccg
//
LOCUS       DAORVN010000114         3829 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_19030_length_3829_cov_3.591150, whole genome shotgun sequence.
ACCESSION   DAORVN010000114 DAORVN010000000
VERSION     DAORVN010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3829
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19030_length_3829_cov_3.591150"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1198
                     /locus_tag="PKV23_03270"
     CDS             <1..1198
                     /locus_tag="PKV23_03270"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HQX84486.1"
                     /translation="KPGPVIISLPEDVLSDEAGDTVSSPFPVAAARHGASDITAIQAM
                     IDAAEHPLIVAGAMLRGAKGAVALERFAEALRIPVAVTWKNQDIFDNGSPLYAGHLGF
                     GNPPKHRQVLGSSDLIIAVGTRLGDVASLNYTFPTAPQPKQKLIHIYPDGEPIGKVFR
                     TDFGLIADPAAVLTDLAAHPRVVSSARESWRSSINEFVREFQRFTSPEPNDAVDFGKV
                     VVSIAAQAPDDAIITTDAGNMSTWVHRHWVMTPKNTLLGAIAGAMGFGVPTAVAAAIV
                     DSTRMAITFVGDGGILMTGQELATAVQYGAKPKVVISDNGIYGTIRTHQERHYPHRVS
                     GTDLVNPDFTLWAKSFGVFTVTIIQGDDIDAKVKEFLAHDGAAVLHVKSSKEALSAFT
                     TLSALH"
     gene            1211..1810
                     /locus_tag="PKV23_03275"
     CDS             1211..1810
                     /locus_tag="PKV23_03275"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HQX84487.1"
                     /translation="MTRTKADLLVLFAALIWGVAFYFQKSAMSHIGPLLFLGLRAILA
                     AAALFPFALREWHEAPTATPPIRPFALLGGTVFFTAAALQQVGIVTATVTNTSFLTAL
                     YVVVTPFLLWLLRHERPGPRIWLASGLAFIGIWALGGGTIGAFSAGDMLIAVSSVAGH
                     STWWSLARRANWQSPCNTRSSIFSWWPPLPCPAPSTSKR"
     gene            1735..2085
                     /locus_tag="PKV23_03280"
     CDS             1735..2085
                     /locus_tag="PKV23_03280"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HQX84488.1"
                     /translation="MQYTFIHFLVVAALALPCAFYFEKVDGAAIADAALPLLYVGILS
                     SAFTFALMANVVRYIPASRASILLSTEVLFSAASGFIMLGERLSPIGWAGAALMFIAI
                     LMIQTASGRTRDKL"
     gene            complement(2082..3692)
                     /locus_tag="PKV23_03285"
     CDS             complement(2082..3692)
                     /locus_tag="PKV23_03285"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HQX84489.1"
                     /translation="MRLIARLLVTVFVLFVGQQAYAAKSDRVPSREGVLGALTEILNA
                     PERQPLPIERVREALKAHYLDNGGSIYWVGTGRMTPFIQRLEDAQFDGLNSGDYPVET
                     LIDVRDSVDPDDPRSAAQAELYYSAFFVAYAADLKIGRVAPQKVDPRLFRSRKSIDVL
                     RVLTDLNKQRNPGTFLSSFEPQNEHYQALKRMMRIYSNQADEGNWPVIGQGVALKPGM
                     SDPRIPRIRSLLALTGDYEWQVTDSPVYDEQLAIAVRRFQQRHGLEAKGLIGKQSVIA
                     MDMKPEERINQIMLNMERWRWMPDSLGDSHFLVNLAAFELQRVQSSVIVERMDVVVGA
                     VATQTPEFSDELEYVELNPTWTVPYSIATKEMLPKLRSNPFAYAADFDVFVNGALSSW
                     GSINWESYGPGKFPFTFRQRPGPKNALGKVKFMLPNKHNIYLHDTPSKDKFASTTRAF
                     SHGCIRLSRPVELAHKLVGEVPGWSGALVDGVLASGKQTRVSLPTHIPVHLVYATAFK
                     GENGIEFRPDVYGRDRKLYNALFGRSAS"
BASE COUNT          748 a         1175 c         1107 g          799 t
ORIGIN      
        1 aaaacccggc ccggtcatca tttccctgcc tgaagatgtg ttgagcgacg aagccggcga
       61 taccgtctct tcccccttcc cggtcgctgc ggcacgtcac ggggcaagcg acatcaccgc
      121 catccaggcc atgatcgatg cagccgagca tcccctcatt gtggccggcg ccatgctgcg
      181 cggggccaaa ggcgccgtgg ccctggagcg gtttgccgaa gccctgcgga ttccggttgc
      241 ggtcacgtgg aaaaaccagg acatatttga caatggctcg cccctctatg ccgggcatct
      301 tggctttgga aatccgccga agcaccggca ggtgctgggc agttcggatc tcatcatcgc
      361 ggttggcacg cggctgggcg atgttgccag cctcaactac acctttccaa ccgctccgca
      421 acccaagcaa aaactcattc acatctaccc cgacggcgag ccaatcggaa aagtgttccg
      481 gaccgatttt ggcctcatcg ccgatcctgc ggcggtcctc acggaccttg cggctcaccc
      541 ccgcgtggtt tcgtcagcgc gtgaatcatg gcgttcatca atcaatgaat ttgttcggga
      601 gttccagcgc ttcacgtcac ccgaacccaa tgatgccgtg gatttcggca aggtggtggt
      661 ctcaatcgcg gcgcaggctc cggacgacgc aatcatcacc acggatgcgg gcaatatgtc
      721 cacctgggtg caccggcact gggtgatgac acccaagaat acgctgctcg gcgccatcgc
      781 gggggccatg ggcttcggcg ttcccactgc ggttgccgcc gcaatcgtgg acagcacccg
      841 catggccatc acctttgtgg gcgacggcgg catcctgatg acgggccagg aactggcaac
      901 cgcggtgcag tatggcgcga aacccaaggt cgtgatttcg gacaacggga tttacggcac
      961 catacgcacg catcaggagc gacactatcc gcaccgggtt tcgggcaccg atctggtcaa
     1021 tccggatttc accctttggg cgaagtcgtt cggtgtgttc accgtgacca tcatccaggg
     1081 cgacgatatc gatgccaagg tcaaggaatt cctggctcat gacggagctg ccgtgctgca
     1141 tgtcaaatcc agcaaggaag ccctgtcggc gtttacaacg ctgagcgccc tgcactgagg
     1201 ctatcccggc ttgacccgca ccaaggctga cctgctggta cttttcgcag cgctcatctg
     1261 gggcgtggcg ttctactttc agaaatcggc catgtcgcat atcgggccgc ttctgtttct
     1321 tgggctcagg gccattcttg ccgccgccgc cctctttccc tttgccctgc gtgaatggca
     1381 tgaagcgccc acggcaacgc cccctatccg cccatttgcc ctgctcggag gcacagtttt
     1441 cttcaccgct gcggctctgc agcaggtggg catcgtcacg gcaacggtca ccaacaccag
     1501 cttcctcacg gcgctctatg tggtggtgac acctttcctg ctctggctgc tgcggcatga
     1561 aaggccggga ccacgcatct ggctggcaag cggcctcgct tttatcggaa tctgggcgct
     1621 gggcggcgga accatcgggg cgttttcagc gggcgacatg ctcatcgcgg tatcatcggt
     1681 ggctggtcac tctacatggt ggtcactagc gcgtcgggca aactggcaaa gcccatgcaa
     1741 tacacgttca tccattttct cgtggtggcc gcccttgccc tgccctgcgc cttctacttc
     1801 gaaaaggtag acggtgctgc cattgcagat gctgccctgc cgcttctcta tgtcggcatt
     1861 ctgtccagcg ccttcacctt cgcgctgatg gccaatgtcg tgcgctacat tcccgcctcg
     1921 cgcgcttcca ttctgctttc aacggaagtt ctttttagcg ctgcctcagg attcatcatg
     1981 ctgggcgaaa ggctgagtcc catcggctgg gcaggtgctg ccctgatgtt cattgccatc
     2041 ctgatgatcc agacggccag cggccgcaca agggacaaac tctaagacgc cgaacgccca
     2101 aaaagcgcgt tatagagttt gcggtcacgg ccgtagacat cggggcggaa ctcaatgcca
     2161 ttttcgccct tgaaggcggt ggcataaacg aggtgcacgg gtatgtgcgt gggcaggctg
     2221 acgcgggtct gcttgccgct ggcgaggaca ccatccacca atgcgccgct ccagcccgga
     2281 acttcgccaa caagcttatg ggcgagttcg acgggacgcg aaagcctgat gcagccgtgg
     2341 ctgaaggcgc gcgtggtgga ggcaaacttg tccttggacg gcgtatcatg caggtagata
     2401 ttgtgcttgt tcggcagcat gaatttcacc ttgcccaagg cgttcttggg gccgggacgc
     2461 tgcctaaagg tgaagggaaa tttccccggg ccataggatt cccagttgat ggaaccccag
     2521 gaagagagcg caccgtttac aaagacgtca aagtccgccg cataggcaaa gggattggaa
     2581 cgcaacttcg ggagcatttc cttggtggca atcgagtatg gcaccgtcca ggtgggattg
     2641 agttccacat attccaactc gtccgagaat tctggggtct gtgtggcaac cgcacccacg
     2701 accacatcca tgcgctcgac aatcaccgag gactgcacgc gctgcaactc aaaggccgca
     2761 agattcacca gaaaatgact gtcgccaaga ctgtcgggca tccagcgcca gcgctccatg
     2821 ttcagcatga tctggttgat gcgctcttcc ggcttcatgt ccatggcgat gacggattgc
     2881 ttgccaatga gaccctttgc ctccagccca tggcgctgct gaaaccttcg cacggcaatg
     2941 gccagctgct cgtcatagac gggtgagtcg gtgacctgcc attcataatc gccggtcagg
     3001 gcaagcagcg acctgatcct gggaatgcgc ggatcgctca tgcccggctt cagcgcgacg
     3061 ccctgcccga tcaccggcca attgccctca tcggcctggt tcgaatagat ccgcatcatc
     3121 cgcttcagtg cctggtagtg ttcattctgc ggctcaaagc ttgacaggaa cgtgccagga
     3181 ttgcgctgtt tgttgagatc cgtcaggacg cgcagcacat cgatggactt gcggctgcga
     3241 aacaggcggg gatcaacctt ttgcggcgcc acccggccga ttttgagatc ggcggcataa
     3301 gccacgaaga atgcggagta atagagttca gcctgggcgg cggaacgcgg atcatcggga
     3361 tccacgctgt cgcgcacatc aatgagcgtt tcgacggggt aatcgccgga attgaggccg
     3421 tcgaactggg catcttcgag acgctggatg aacggcgtca tgcgcccggt gccgacccaa
     3481 tagatggagc cgccattatc caggtaatgc gcctttagcg cctccctgac gcgctcgatc
     3541 ggcaggggct ggcgttcggg cgcgttcaat atctcggtca gggccccgag aacgccttca
     3601 cggcttggca cacggtcact ttttgcggca taggcctgct gaccgacgaa caggacgaac
     3661 actgttacca aaagacgtgc aatcaggcgc atgaaagctc cgctgccccg gcgctagaca
     3721 ttgagcagca ggaattcccg ctcccaggaa ctgatgacct tgcgaaagtg ctgcatttcc
     3781 tcaaacttca catcctggta ggcagtgacg aatttctctc ccatcacct
//
LOCUS       DAORVN010000115         3819 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_19126_length_3819_cov_2.987513, whole genome shotgun sequence.
ACCESSION   DAORVN010000115 DAORVN010000000
VERSION     DAORVN010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3819
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19126_length_3819_cov_2.987513"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(363..1175)
                     /locus_tag="PKV23_03290"
     CDS             complement(363..1175)
                     /locus_tag="PKV23_03290"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014490718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HQX84490.1"
                     /translation="MTYSQICYDVIRAVATITLNRPERLNAWTPTMAAEFRQAMEQAN
                     NDPQVRAIIVTGAGKGFCSGVDMNALDGMTSSGKTPSKSFTPLDPAAREDFQHPMTWL
                     PSVGKPIIAAVNGAAAGLGLALLLFCDLRFASSNAAFVTSFSRRGLIAEHGTSWMLTR
                     LVGHSRALDLMFSSRRVDGDEAYRIGLADRVCAPERLLDEAREYAENLAANVSPRSTR
                     IMKRQLWDALFVDLATSMQEADEEMRESLKSADFKEGVAHFLEKRPAKFVGG"
     gene            complement(1216..1959)
                     /locus_tag="PKV23_03295"
     CDS             complement(1216..1959)
                     /locus_tag="PKV23_03295"
                     /inference="COORDINATES: protein motif:HMM:NF024859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HQX84491.1"
                     /translation="MKLRLRQIALAVTDLEVATLQASQILGLGSAYQDPAIVRYGLRN
                     AVYAIDNTFLEFVSPITENTTVGRLLAKQGGDCGYMVILQTDGADEARARIDAAGVRV
                     ADQLDRNGAGFMHLHPKDVGGTLLSIDYMPRWSDWEWGGPDWQKSATHDEAITGIGIS
                     ALDPQGMAGRWGEILGRPMEHCDHASRIGLDEGELRFAAPAGNGSEGFQAFDLHSARA
                     AQLRENAVRHGCLTSEGNIALFGVTIRLV"
     gene            complement(1998..2681)
                     /locus_tag="PKV23_03300"
     CDS             complement(1998..2681)
                     /locus_tag="PKV23_03300"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HQX84492.1"
                     /translation="MHEDRAQHEPGVLSPELDAALRQHARRRLLGRGEQLYAFGSVPD
                     ALFCVEEGVLRLSVTSPEGRESVLSMVTQNQWFGEASLFSGEPRGNDAMAIVASAVLI
                     VPAAAVHQLVDHRPDFLLQFLAMMGRRYRMILSRMDDTVLRPLPARLAREVLQAHGRE
                     LAAAGPHHPVTLRFSQEEMSQMLGASRQSINRLLKQWERGGAIEVAYRSLTVLDPEAL
                     RLLTERDEG"
     gene            complement(2719..3630)
                     /locus_tag="PKV23_03305"
     CDS             complement(2719..3630)
                     /locus_tag="PKV23_03305"
                     /inference="COORDINATES: protein motif:HMM:NF004843.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HQX84493.1"
                     /translation="MKNMKGGVAVVTGGGSGLGKAIAQEAARRGMRVVIADVQAEAMN
                     AAVKELRASGADAIGVLTDVTSAASVEALAEAAERQFGPANLVFNNAGVASGGPIWES
                     TEKDWKWVFAVNVDGVANGVRSFTPRMLAAAAADPAYQGCIVNTASMAGLVTAPGMGI
                     YSVSKHAVIAISECLYHDLDLAGDQVKTAVLCPSYVTTNISQCYRTRPADLANESGPT
                     KSQLATQAISAKDLANGSLTAEEVASITFQAIEVGQFYVYPSPELLPIVKNRLDNIAA
                     GSNPDIPYEDIPMFKERRDRLKEALAS"
BASE COUNT          720 a         1182 c         1131 g          786 t
ORIGIN      
        1 ttccggaata ggttcattgt aggttgcgac atgtagtggt agcgccatag taaacactcc
       61 tccaccagga gattgagcgg acggaatgcc cgctctcgag tcctggtagc gcgaagctat
      121 caagttccga ttgtgttctt tagcacttcg agtcgaaagc ccgtcaggag agttttggca
      181 gttatgttgg acgatccgga aagtctcctt tcaggaccgc aaattgggat agcggacatt
      241 ttcatgcata gttgaggcaa actccccgtg gccattccgg ccacaggaga agtgtcatgg
      301 gacatttaga cctcgaaggc attccagata ccgatgtcga tcacaaaacc ccaaaataaa
      361 ctttagccac ctacgaactt tgccgggcgc ttctcaagga agtgcgcaac gccctccttg
      421 aaatccgcgc ttttcaggct ttcccgcatt tcctcatcgg cttcctgcat cgaggttgcg
      481 agatcgacga acagggcgtc ccacagctgg cgcttcatga tgcgggtcga gcgcggagag
      541 acattcgccg ccaggttctc ggcatattcg cgtgcctcgt ccagcagccg ttcgggggcg
      601 cagacccggt ctgccaggcc gatgcggtat gcctcgtcgc catctactcg acgcgaggag
      661 aacatcaggt ccagtgcacg gctatgtccc accaatcgcg tcagcatcca gctggtgcca
      721 tgttccgcga tcaggccgcg ccgcgagaat gatgtcacaa atgccgcgtt gctggaggca
      781 aaccgcaaat cgcagaacag cagaagcgca agccccagcc cggccgccgc accgttgacc
      841 gcggcaataa tcggcttgcc gaccgacggc agccaggtca tcggatgctg gaaatcttcg
      901 cgggccgccg ggtcaagcgg ggtgaacgat ttgcttgggg tcttgccgct cgaggtcatt
      961 ccatccagcg cgttcatgtc gacgcccgag cagaacccct tgcccgcgcc ggtcacgata
     1021 atcgcacgca cctgcggatc gttgttcgcc tgctccatgg cctggcggaa ttctgccgcc
     1081 atggtcgggg tccaggcgtt caggcgctct ggccggttca gcgtgatggt ggcgaccgcg
     1141 cggatcacat cataacaaat ctgcgaatag gtcactggtt atcctctcgt gtctcaggcg
     1201 ctttgatgca ggctatcaaa ccagccgaat agtcacgccg aacagtgcga tattcccctc
     1261 actggtcagg cagccgtgcc gaacagcgtt ttcgcgcagc tgcgcggcgc gggcggagtg
     1321 caggtcgaag gcttggaatc cttcactgcc gttgcctgct ggggccgcaa accgcagctc
     1381 gccttcatcc aggccgatgc ggcttgcatg atcgcaatgt tccatcgggc gaccgagaat
     1441 ttccccccag cgccccgcca tgccctgcgg gtcgagcgcg cttattccaa tcccggtaat
     1501 ggcctcgtca tgggtagcgg acttttgcca gtcggggccg ccccattccc agtcggacca
     1561 gcgcggcatg taatcgatgg acagcagggt tccgccaaca tccttgggat gcaggtgcat
     1621 gaagcccgcg ccattgcggt ccaactgatc cgccacgcgc acacccgcag catcaattcg
     1681 cgcgcgcgct tcgtctgccc cgtcggtttg aagaatgacc atatagccgc agtcaccgcc
     1741 ttgcttggcc agcaagcgcc cgacggtggt gttttcggtg atcggcgata cgaattccag
     1801 aaaggtgttg tcgatcgcat agaccgcatt gcgcagcccg taacgcacga ttgccgggtc
     1861 ttgataggct gagcccaggc caagaatctg gctggcctgc aatgttgcaa cttcaaggtc
     1921 agttacggcg agtgcaatct ggcgcaggcg cagtttcatc ttggtactcc ggtagtttgg
     1981 agcaggacca tagcgcttta tccttcgtca cgttccgtga gcaaccgcag cgcttcagga
     2041 tcgaggactg tcaggctgcg gtaagcaact tctatggcgc cgcctcgctc ccattgcttg
     2101 agcaggcggt tgatgctttg ccgcgatgcg ccgagcatct ggctcatttc ctcttgcgag
     2161 aagcggagcg tgaccgggtg atgtggtcct gccgctgcaa gctcccgccc atgcgcctgc
     2221 agcacttccc gcgccaggcg ggcgggcaag gggcgcagca cggtatcgtc catccggctg
     2281 agtatcatgc gataccgcct ccccatcatg gccaggaatt gcagcaagaa atccggccgg
     2341 tggtcgacaa gctggtggac tgctgcagcc gggacgatca gcacagcgct ggcaacaatc
     2401 gccatggcgt catttccgcg cggctctccc gaaaacaggg aggcttcgcc gaaccactgg
     2461 ttttgagtca ccatgctgag aacggattcc cggccctcgg ggctggtcac gctgaggcgc
     2521 agtactccct cttcaacgca gaacagcgca tcgggcacgg acccgaacgc ataaagctgc
     2581 tcacccctgc caagcagtcg gcggcgggca tgctggcgca gcgcagcatc gagctccggg
     2641 ctcaatactc ctggttcgtg ctgggccctg tcttcgtgca actgccgatg tccttgatcc
     2701 gcctgtgagg gctggcgttc aactggccag tgcttccttt aaccggtcgc gccgctcctt
     2761 gaacatcgga atgtcctcgt agggaatatc gggattggat cccgccgcga tgttatccag
     2821 gcggttcttg acgatcggga gcagttccgg gctgggatag acgtagaact gtcccacttc
     2881 gattgcctgg aaagtgatgc tggccacttc ttctgcggtc aacgacccat tggcgagatc
     2941 cttcgcggaa atggcctggg tcgccagctg ggactttgtc gggccgcttt cattggcaag
     3001 atcggcgggc cgggtgcgat agcattggct gatgttggtg gtcacgtatg agggacacag
     3061 cacagctgtc ttgacctgat ctccggccag atcgagatcg tggtaaaggc attccgaaat
     3121 cgcgatcacg gcgtgtttgg aaacgctgta aatgcccatg ccgggtgccg taaccagtcc
     3181 ggccatcgag gcggtattca caatgcagcc ttgataggcc gggtcggcag cggcggcagc
     3241 aagcatccgc ggggtgaagc tccgcacacc gttggccacc ccatcgacgt tgacggcgaa
     3301 cacccacttc caatcctttt cggtgctttc ccagatcggc ccaccggatg cgaccccggc
     3361 attgttgaac accaggttgg cggggccaaa ctggcgttcg gcggcctctg cgagcgcctc
     3421 aaccgaagca gcgctggtca cgtcggtcaa cacaccaatc gcatccgcac cgctggcccg
     3481 cagctcttta accgccgcat tcatcgcctc ggcctgcacg tcggcaatca ccacccgcat
     3541 tccccggcgt gcggcctcct gtgcaatagc cttgcccagg ccagaaccgc cgccggttac
     3601 tacggctacg ccgcctttca tgttcttcat ctgcacgctc ctcggtcttt gcgggtccgg
     3661 cccggtcaat agctgtagga aaagatcgga tcgtcgagat cgatggccgg gatggccttg
     3721 ctctcgtaga gataatcctg gctgtgctgc cactccggct tgtcaccgct gcgtggcaag
     3781 cggtccaggc tgcgcaagag gtagccggga ttgaggtat
//
LOCUS       DAORVN010000116         3810 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_19214_length_3810_cov_3.146738, whole genome shotgun sequence.
ACCESSION   DAORVN010000116 DAORVN010000000
VERSION     DAORVN010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3810
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19214_length_3810_cov_3.146738"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..694)
                     /locus_tag="PKV23_03310"
     CDS             complement(<1..694)
                     /locus_tag="PKV23_03310"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003600719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HQX84494.1"
                     /translation="MSILPFDQREGSIWFDGQIVPWKEARVHVLTHGLHYGSSIFEGE
                     RAYAGTIFKSREHSERLHKSAAIMDFVIPYSVEQLDAAKARVVELNGGGDQYVRPVAW
                     RGSEMMAVSAQHNTIHVAIASWTWPSMFDPETKMKGIRLDIADYRRPDPQCAPVHAKA
                     AGLYMICTISKHKAEKKGYADAMMLDWQGRVAECTGANIFFTRDGAIHTPIADCFLNG
                     LTRQTVIALAKQQ"
     gene            827..1339
                     /locus_tag="PKV23_03315"
     CDS             827..1339
                     /locus_tag="PKV23_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012320996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HQX84495.1"
                     /translation="MADINFDDTSGENETVALIELLFFAYRDFVSDPDAVLEQLGFGR
                     AHHRVVHFVGRYPGMTVAQLLDILRITKQSLGRVLKDLIDKGYVFQKEGETDRRQRLL
                     HLTKAGEELRQQLMAPQISRIRRAVSETAGNGKSSMRDILYHLVSPENREGVKDWIDG
                     ARKLGEKAGD"
     gene            1302..2027
                     /locus_tag="PKV23_03320"
     CDS             1302..2027
                     /locus_tag="PKV23_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019565223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQX84496.1"
                     /translation="MAPASSERKPVTEARHILVVDDDRRIRELIRSYLMENDFLVSVA
                     GTAAEARERMRGVTFDLMVLDIMMPGETGLELTKSLRGEKRNVPILMLSALADSDDRI
                     AGLATGSDDYLAKPFEPRELLLRINNLLRRVSVAAPARTEVRFGVFQLNVPRGELRRN
                     GDLVRLTSAEKNLLRMLSLKPGTPFSRLELSQPGLEESARSVDVQINRLRQKIESDPA
                     NPVYLQTVRGAGYALYVDEEAAG"
     gene            2024..3364
                     /locus_tag="PKV23_03325"
     CDS             2024..3364
                     /locus_tag="PKV23_03325"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQX84497.1"
                     /translation="MSSVYFRFNRFLERHLPKGLYSRSLIIVIAPIVLLQTIMAGIIL
                     ERHWDNVTKVLGRSLAREIGLITDLYDRSDKSEAAIMEIESVANNRLKLGLEIARGAT
                     LPAPIDRTLYSLVDDKMTKYLERETGRPFWINSAAKGGKVEIQVEAEKGLIFRIRTDE
                     DRAYAANTGVLLLIMLLSSLVLLFIAVVFLRKQITPIVELANAAKSFGMGREVSEFRP
                     RGAAEVRDAGLAFVDMKSRIARHVEQRTAMLAGVSHDLRTILTRFKLELAFLGDGPKI
                     KPLKEDVEEMQRMLEAYMAFVRGDGGEKTEAVNLAQMFSSVASAVGRGKSHVALDVQD
                     VDVSIKPNAFRRLLSNLLGNAVRYADNVKVKGGVENRLLTILVDDDGPGIPAKNREDA
                     FRPFVRLDHARNLDETGTGLGLAIALDIAHAHGGEIRLDDSPTGGLRAVVQIPV"
     gene            complement(3361..3699)
                     /locus_tag="PKV23_03330"
     CDS             complement(3361..3699)
                     /locus_tag="PKV23_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HQX84498.1"
                     /translation="MPQISFDDFLKVDVRVGTIIAAEPFPEARKPAFKLRIDFGAEIG
                     VKKSSAQVTKYYTPETLLGRQVAAVVNFPPRQIGPFMSEVLTLGFPDPAGEVVLVAVE
                     RPVPNGGRMY"
     gene            complement(3700..>3810)
                     /locus_tag="PKV23_03335"
     CDS             complement(3700..>3810)
                     /locus_tag="PKV23_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007198518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HQX84499.1"
                     /translation="GTTYAALQVLMADDGLQPLFEKAVKAAADRSRELAS"
BASE COUNT          763 a         1096 c         1199 g          752 t
ORIGIN      
        1 cctgctgttt ggcaagggca atcacggtct ggcgcgtcag gccattgagg aagcaatcgg
       61 cgatcggcgt gtggatggcg ccgtcgcggg tgaagaaaat attggctccg gtgcattcgg
      121 ccacgcggcc ctgccagtcc agcatcatgg catcggcata gcccttcttc tcggccttgt
      181 gcttggagat ggtgcagatc atgtagaggc ccgccgcctt ggcatgaacc ggcgcgcatt
      241 gcggatcagg cctgcggtaa tcggcgatgt cgagcctgat gcctttcatc ttggtttcgg
      301 gatcgaacat ggaaggccag gtccagctgg caatggccac atggattgta ttgtgctggg
      361 cggagaccgc catcatctcg gaaccgcgcc aggcaacggg gcggacatac tggtcgccgc
      421 cgccgttcag ttccacaacc ctggccttgg ccgcgtcaag ctgctcgacg gagtagggga
      481 tcacgaaatc catgatcgcg gcggatttgt gcaggcgctc ggaatgttcg cgcgatttga
      541 aaatggtgcc ggcataggcg cgctcgcctt caaaaatcga ggagccatag tgcaggccgt
      601 gggtgaggac gtggaccctg gcttccttcc agggcacgat ctggccatcg aaccagatgg
      661 aaccttcacg ttggtcaaaa ggcaggatgg acatcggaac tctcccaatt cgatttttat
      721 agaacttcag cctatattac ggtcaaacct gcgaaatcag cggtttacgc aataacattg
      781 cgtgttattg agatgcaacg taacaaaaat ggattaatat gtcaacatgg ctgacataaa
      841 ttttgacgac acatcggggg agaatgaaac cgtggctttg atcgagctgc tgttcttcgc
      901 ctaccgggat ttcgtctcgg atccggatgc ggttctggag caactgggct ttggccgggc
      961 gcaccaccgg gtggtgcatt ttgtcggccg ctacccgggc atgacggtgg cgcagctgct
     1021 cgatatcctg cggatcacca agcagagcct gggccgggtt ctgaaagacc tgatcgacaa
     1081 gggctatgta ttccagaaag aaggcgagac ggaccggcgc cagcggctgc tgcatctcac
     1141 caaggcaggc gaagagctgc gccagcaact catggcgccg cagatttcac gcatccgccg
     1201 ggcggtgagc gagactgccg gcaatggcaa gtcttcgatg cgtgacattc tctatcacct
     1261 ggtcagcccg gaaaaccgcg agggcgtcaa ggactggatt gatggcgccc gcaagctcgg
     1321 agagaaagcc ggtgactgag gcccggcaca ttctggtggt ggacgatgac cgccgaatcc
     1381 gcgagctgat caggtcctat ctgatggaga atgattttct ggtgtccgtc gcgggaaccg
     1441 cggctgaagc ccgcgagcgc atgcgcggcg tcacctttga cctcatggtg cttgatatca
     1501 tgatgcccgg tgaaacgggg cttgaactca ccaaatccct gcgcggcgaa aaacgcaacg
     1561 ttccaatcct catgctttcg gcgctcgccg attctgatga ccgcatcgcc gggcttgcca
     1621 cgggcagcga tgactatctg gcaaagccct ttgagccgcg cgagctactg ctgcgcatca
     1681 acaacctgct gcgccgcgtg agcgttgccg cgcctgccag gacggaggtg cgctttgggg
     1741 tttttcaact caatgtgccg cgcggcgagc ttcgccggaa tggcgacctg gtgcggctga
     1801 cctcggctga aaagaacctg cttcgcatgc tgagcctcaa gcccggaacg ccctttagcc
     1861 gccttgagct ttcgcaaccg gggctggagg agagtgcgcg cagtgttgac gtgcagatca
     1921 accggctgcg ccagaaaatc gaaagcgatc cggccaaccc ggtgtacctt cagacggtgc
     1981 gcggggcggg atatgcgctc tatgtcgatg aagaggcagc tggatgagca gcgtttactt
     2041 ccgcttcaac cgctttctcg agcggcatct gccgaagggg ctctactccc gttcactgat
     2101 cattgtcatt gcgcccatcg tgctcctgca gacgatcatg gcagggatca ttcttgagcg
     2161 gcactgggac aatgtcacca aggtcctggg ccggtcgctt gcccgcgaaa tcggccttat
     2221 caccgatctc tatgaccgtt cggacaaatc cgaagcagcg atcatggaaa ttgaatccgt
     2281 cgccaacaac cgcctgaaac tcggacttga gattgcccgc ggcgctacgc tgccggcgcc
     2341 catcgaccgc accctgtatt cgctggtcga tgacaagatg acgaaatacc tggagcgtga
     2401 aaccggccgg ccgttctgga tcaacagcgc ggcgaaaggc ggcaaggtgg aaatacaggt
     2461 tgaggccgag aagggcctga tcttccgcat ccgcaccgac gaggaccggg cctatgccgc
     2521 caacaccggg gttctgctgc tgatcatgct tctgtcttcg ctggtgctgc tgttcatcgc
     2581 tgttgttttc ctgcgaaagc agatcacccc cattgttgaa ctggcaaacg ccgccaagag
     2641 tttcggcatg gggcgcgagg tcagcgagtt ccgcccgcgc ggtgccgctg aagtgcgcga
     2701 tgcagggctg gcctttgtcg acatgaaaag ccgcattgcg cgccatgtgg aacagcggac
     2761 cgccatgctg gcgggcgtct cgcatgatct ccgcaccatt ctcacgcgct tcaagctgga
     2821 actggccttt ctcggtgacg ggccgaaaat caagccgctc aaggaagacg tggaggagat
     2881 gcagcgcatg ctggaagcct atatggcctt tgtgcgcggc gatggcggcg agaagacgga
     2941 agcggtcaac ctggcgcaaa tgttcagctc ggtcgcctcc gcggtgggcc ggggcaagtc
     3001 ccatgtggcg ctcgacgtgc aggatgtgga cgtcagcatc aagcccaacg cgttccggcg
     3061 cctgctttca aaccttttgg gcaacgcggt gcgttatgcc gataatgtga aggtgaaggg
     3121 cggggttgaa aaccgcctgc tcaccatcct tgtcgacgat gacgggccgg gcattccggc
     3181 caagaaccgc gaagatgcat tcaggccatt tgtgcggctg gaccatgccc gcaaccttga
     3241 tgaaacgggc acgggccttg ggctcgccat cgccctggac atcgcccatg cccatggtgg
     3301 ggaaatcagg ctggatgaca gcccaacagg cgggcttcgc gccgtggtgc agatcccggt
     3361 ctagtacatc cttccgccat tgggcacggg gcgctcgacc gccaccagca ccacctcgcc
     3421 cgcaggatcg ggaaagccga gggtcaggac ttccgacatg aaggggccga tctggcgcgg
     3481 cgggaaattg acgacggcgg ccacctggcg gccaagcagg gtttccggcg tgtagtattt
     3541 ggtcacctgg gcagacgatt ttttcacgcc gatctcggcg ccgaaatcaa tgcgcagctt
     3601 gaaagcaggc ttgcgcgctt ccgggaaagg ctcggcggcg atgatggtgc ccacgcggac
     3661 gtcaaccttg aggaagtcat cgaaggaaat ttgcggcatc tagcttgcca gctcccgcga
     3721 acggtcggct gccgccttca cggccttttc gaagaggggc tgcaggccgt cgtctgccat
     3781 gagaacctgg agggccgcat aggttgtgcc
//
LOCUS       DAORVN010000117         3805 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_19257_length_3805_cov_3.445067, whole genome shotgun sequence.
ACCESSION   DAORVN010000117 DAORVN010000000
VERSION     DAORVN010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3805)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3805)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3805
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19257_length_3805_cov_3.445067"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..488)
                     /locus_tag="PKV23_03340"
     CDS             complement(<1..488)
                     /locus_tag="PKV23_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012331951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HQX84500.1"
                     /translation="MNWVKAGFVVVVASVAAAGAAEAAKCSSTGGGFEAWKKSFAVEA
                     KANGVKPKAISALMGTSYSKGTIRADRGQHGFKLSLKAFMAKRGADGIVARGRTLKKA
                     NAALFAKLEKRYGVPPGPLLAIWGMETGFGGFLGNQNTLSAVATLAYDCRRTAFFTDH
                     LYA"
     gene            577..1176
                     /locus_tag="PKV23_03345"
     CDS             577..1176
                     /locus_tag="PKV23_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009764662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02466 family protein"
                     /protein_id="HQX84501.1"
                     /translation="MKSTVESLFPTLVYRAEVSNAQRLNLELEDAALSLSQHDARGRR
                     WCEKHGYAGYTSYASIDDLSRRSKPFARLEKIIHAHALDFARSLQWNLRGQKPVCDSL
                     WVNVLPQGGSHTSHIHTNAVLSGTYYVTAPEGASPIVFEDPRHAMMMAAPPRKPGIYE
                     SRTPKAGTLMLWESWLRHEVPMNRSPEDRISVSFNCVIG"
     gene            1280..2074
                     /locus_tag="PKV23_03350"
     CDS             1280..2074
                     /locus_tag="PKV23_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014329721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQX84502.1"
                     /translation="MRFLAAFCLAMALLSTSGNAQALSSDCIAVAQGPARVQYAKMVP
                     AALTENQVQLTFIGHSTFLIESSGGLRIATDYSGNAGGVVPDVVTMNRAHRSHYTPTP
                     DPAIKNVLRGWNDDGTPAEHSVTFGDVHIRNVTTDIRGGTLGRVPDGNSIFVFEVAGL
                     CIGHLGHLHHELTPGHIGQIGRLDVVLVPVDGGYTMPVVNMIQVLKDLKTRLVIPMHY
                     FGPTTLSRFIGNVRGSLEIEMASSPVVIVSRETLPEEPKLIVLPGY"
     gene            2118..2429
                     /locus_tag="PKV23_03355"
     CDS             2118..2429
                     /locus_tag="PKV23_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003605507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HQX84503.1"
                     /translation="MRIILSLFLLVFTSSLSRADDIAAGRKLVEANCSRCHAIGMEGG
                     SPLATAPPFREVVTRYPPDSLGEALVEGIVTGHNEMPEFVFTADQASEIIAYLDSLVT
                     K"
     gene            complement(2430..2726)
                     /locus_tag="PKV23_03360"
     CDS             complement(2430..2726)
                     /locus_tag="PKV23_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018073449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HQX84504.1"
                     /translation="MIRHTAVFRLKHAAGSAGERDFLAANRALKAIPGVEAFELLRQV
                     SPKNDFTFGVSMEFADDAAYQGYNNHPAHVAFVRDRWVPEVAAFMEIDYVALPE"
     gene            complement(3003..3311)
                     /locus_tag="PKV23_03365"
     CDS             complement(3003..3311)
                     /locus_tag="PKV23_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013529693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HQX84505.1"
                     /translation="MIRHVVLCRFRRDVSEVAIAQIFAALKALQHQIDGIIAITAGRD
                     NSPEGLQKGCSHGFTVDFVDAAARDGYLPHPAHQKVGAMIVAAAEGGVAGITVVDWDV
                     "
     gene            complement(3312..>3805)
                     /locus_tag="PKV23_03370"
     CDS             complement(3312..>3805)
                     /locus_tag="PKV23_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009029389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HQX84506.1"
                     /translation="IKQKRVGLKNFSMIPNGTGGLEDRMPVLWNAGVNTGRLTKEEFV
                     GVTSANIARILNMYPRKGAVAVGSDADIVIWDPAATKKISARNQVSRIEYNVFEGYEC
                     KGAPRTVLSRGRVAWEQGDIRAKPGDGDFVARQPNAPVHQANAIWKGVTAPKGVKRVE
                     VTP"
BASE COUNT          771 a         1235 c         1030 g          769 t
ORIGIN      
        1 gcatagagat gatcggtgaa gaacgcggtg cggcggcagt cataagccag ggttgccacc
       61 gccgacaggg tgttttgatt acccaaaaaa ccgccaaagc ctgtttccat gccccagatc
      121 gcaagcaacg ggccgggtgg aacgccgtaa cgtttttcaa gctttgcaaa gagcgctgca
      181 ttggccttct tgagcgttcg cccccgcgcg acaatcccgt cggccccgcg cttggccatg
      241 aaggccttga gcgacagctt gaaaccatgc tgcccccggt cggcgcggat ggtgcccttc
      301 gaatagcttg tgcccatgag ggcggaaatc gccttgggct tcaccccgtt ggcctttgcc
      361 tcaaccgcaa agcttttctt ccacgcttca aaaccacctc ccgttgagct gcatttggcc
      421 gcttcggccg cacccgccgc agcaaccgac gccaccacaa caacaaatcc cgccttcacc
      481 cagttcattc ctgctccact ctttgcctgt tcggatccca gactgccatt atcgccagcc
      541 cttgtcagca gggagcaagc tgattaaggg acagccatga aaagcaccgt ggaaagcctg
      601 tttcccaccc tggtttaccg ggccgaggta agcaatgccc agcgcctgaa tctcgaactg
      661 gaagatgctg ctctgtcctt gagccagcac gatgccaggg gcaggcgctg gtgcgaaaag
      721 cacggctacg ccggctatac ctcctatgcc tcaattgatg atctcagccg gcgctcaaaa
      781 cccttcgccc ggctggaaaa aatcatccat gcccatgcct tggattttgc caggtccctg
      841 cagtggaatt tgcgtggaca gaaacctgtc tgcgattcac tctgggtgaa cgtcctgccg
      901 cagggcggtt cgcacacctc gcacattcac accaacgcgg ttctaagcgg aacttactat
      961 gtaaccgccc cggaaggcgc cagccccatc gtcttcgagg acccgcgcca tgccatgatg
     1021 atggcagcac ccccgcgtaa gcccggcatt tatgaaagcc gcacgcccaa ggccggaacc
     1081 ctgatgctct gggaaagctg gctcaggcat gaagtgccaa tgaaccgctc gcccgaggac
     1141 cggatcagcg tgagcttcaa ctgtgtgatt gggtagtcaa cacttttccg tgagcagcca
     1201 tctctccatt taaatttctg ccacaatgga agggcgctat aacacttgaa cttccgtttc
     1261 aggagagccg ccgatgccca tgaggtttct tgccgcgttc tgtcttgcga tggccctgct
     1321 ttccacaagc ggaaatgccc aggccctgtc gagcgactgc atagccgtgg cccagggccc
     1381 cgcgcgcgtg caatacgcaa aaatggttcc cgccgctctg accgaaaacc aggtgcagct
     1441 cacctttatc ggccattcaa ctttcctgat cgaaagctcc ggcgggcttc gcattgccac
     1501 ggattattcc ggaaatgccg gcggcgttgt gcctgatgta gtcaccatga accgtgccca
     1561 ccgctcgcac tacacaccaa cccctgatcc ggcgatcaag aatgtcctgc gcggctggaa
     1621 cgacgatggc accccggcag agcacagtgt caccttcggt gacgttcaca tccgcaatgt
     1681 gaccaccgac atccggggcg gcaccctggg ccgcgtgccc gatggcaatt cgattttcgt
     1741 ctttgaagtg gcgggcctct gcatcggcca cctcggccac ctgcaccacg agctcacgcc
     1801 cggccatatc ggccagatcg gcaggctcga cgtggttctg gtgcctgtcg acggcggtta
     1861 caccatgccc gtcgtcaaca tgatccaggt tctgaaggac ctgaagaccc ggctggtcat
     1921 ccccatgcac tatttcggcc ccacgaccct gtcgcgcttc atcggaaatg tgcgggggtc
     1981 ccttgaaatc gaaatggcgt cctcgcccgt ggtcatcgtc tcgcgcgaaa ccctccccga
     2041 ggagcccaag ctgattgtcc tgccgggcta ttgatatggg tcaatgcgat agccttcgcc
     2101 catgctatcc tttttagatg cgcatcatcc tcagcctttt cttgctcgtt ttcaccagtt
     2161 ccctctcccg cgccgacgat atcgccgcgg gccgcaagct ggttgaagcc aattgcagcc
     2221 gctgccatgc gattggcatg gagggcggaa gcccgctggc cacggccccg ccgtttcgcg
     2281 aagtggtgac gcgctatccc ccggactccc tgggcgaggc cctggtcgag ggcattgtca
     2341 ccggccacaa cgaaatgccc gagttcgtct tcacggcgga ccaggcttcg gagatcatcg
     2401 cctatctcga cagcctcgtg acgaagtagc tattccggca gcgcgacgta atcaatttcc
     2461 atgaaggccg ccacttccgg aacccagcgg tcgcgcacaa aagccacatg ggccggatgg
     2521 ttgttgtagc cctggtaggc cgcatcgtca gcaaactcca tcgacacgcc aaaggtgaaa
     2581 tcattcttgg ggctcacctg gcgcaggagc tcaaaggcct cgacgccggg aatggccttg
     2641 agcgcccggt tggcggcaag aaagtcacgc tcgccggccg aacccgcggc atgcttgagg
     2701 cggaacacgg cagtatggcg gatcatgggg aaactccgat tgtggcggaa aagttgcgaa
     2761 acactatttc accgctctat tcggtgcaag tacctacctg caaaaaatag gaattaaagc
     2821 ttgacaccgc acggtcattc tgccatatat ggctagtctc caaaattgta agtttcaccc
     2881 gtgagcgcaa ggccctctaa acaggggcat cgctgaaatc caatcggctt tggccatggt
     2941 ccaacactgg caaaatcttg tttggaagag agacaccgtg cgggcagcct aaaccatcag
     3001 cgtcacacgt cccaatcgac aaccgtaatt cccgcaacac cgccctcggc ggccgccacg
     3061 atcatcgcgc caaccttctg atgggcggga tggggcagat agccgtcacg cgccgcggca
     3121 tctacgaaat ccacggtgaa gccatggctg cagcccttct gcaatccttc gggactgttg
     3181 tcgcgccccg ccgtgatggc gatgatccca tcgatctgat gctgcaacgc cttgagggcg
     3241 gcaaaaatct gcgcgatggc aacttcgctc acatctctgc ggaaccggca aaggacaaca
     3301 tggcggatca tctaaggtgt aacctcaacc cgcttcacac ccttcggcgc ggtgacacct
     3361 ttccagatcg cgttggcctg gtgcaccggc gcgttgggct gcctcgccac gaaatcgccg
     3421 tcgcccggct tggcgcggat gtcgccctgc tcccaggcga cgcgtccgcg cgacagcacc
     3481 gtgcgcggcg cacccttgca ctcatagcct tcgaagacat tatattcgat gcggctcacc
     3541 tggttcctgg cgctgatctt cttcgtggcg gcgggatccc agatgacaat gtcggcatcg
     3601 ctgcccaccg ccaccgcgcc cttcctggga tacatgttca agattcgcgc gatgttcgcc
     3661 gaggtgacgc ccacgaattc ttccttggtc agccgcccgg tgttcacccc ggcattccac
     3721 aaaaccggca tgcggtcttc aagcccgcct gtgccgttgg ggatcatcga gaaattcttg
     3781 aggccaacac gcttctgttt gatgg
//
LOCUS       DAORVN010000118         3796 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_19329_length_3796_cov_3.252874, whole genome shotgun sequence.
ACCESSION   DAORVN010000118 DAORVN010000000
VERSION     DAORVN010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3796
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19329_length_3796_cov_3.252874"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..411)
                     /locus_tag="PKV23_03375"
     CDS             complement(<1..411)
                     /locus_tag="PKV23_03375"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018389501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HQX84507.1"
                     /translation="MTAHKRIRPFNTKETYPEQKLDNDLCQAVVARGTMVFVRGQVSQ
                     DLDTRESLHVGDAAAQTAKTMANIKLLMEEAGGNMDHICRIVVYLTDIRYRQDVYREM
                     GKWLKGVFPCSTGLVITALARPEWVVEIEVTAVIP"
     gene            complement(411..1877)
                     /locus_tag="PKV23_03380"
     CDS             complement(411..1877)
                     /locus_tag="PKV23_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008139126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase family
                     protein"
                     /protein_id="HQX84508.1"
                     /translation="MIRTGEQYRESLRDGREVWINGERVRDVTRHPMFKPVVDIRARI
                     YDMQHEAAHREVMTYEEKGERFAIGNRLPRAQQDWTDKRKAVDAVMYEVGGVVTRVGD
                     ETVGEMWSLWDGKDILNEIDPRFAANIERHIHESIAHDPFHVSANTDPKGDRSKPPQE
                     QDPDMLVHVVKETDAGIIIRGAKYETAAAYANQAFLKPTIANWGDAKLSEYALGCIVK
                     MNAPGVKHICRTGFTGRAPERDYPLSNKFDEVDTIVILDNVLIPWEDILFYQHTRAAA
                     FIRQTLHRYSAFPFVQRTLSYADLIIGAALWNARQTGLDKQQAVQEKLSELAVWREGI
                     NAHLTASIVMAEKSPNGLLMPNQSLLMSGRCHALNNLPRMMHIARELCGGQICVTPDA
                     AVFEHPETKKWMDKYYTINDEWLADDRRKLLVLARDLLNSDYAGHRLTFQLFAQAPPF
                     AQSAAVYRTFDWNSTLKFAQKAGGMSPRIWGKQKQAAE"
     gene            2268..3518
                     /locus_tag="PKV23_03385"
     CDS             2268..3518
                     /locus_tag="PKV23_03385"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HQX84509.1"
                     /translation="MSHPHATIIGAGIVGVSSAAFLQRAGYRVTLIDRLPPGEGCSFG
                     NAGGLAFCEIVPPIHPRILLKIPGWLMDPLGPLTIRWSYLPKALPWLLAAGRNAMPAR
                     VAAITEARANLALRVVADFETLLRDAGSAELLKYEDTLRLYDNEQQFAAEANEREVKK
                     AYGFEMKRLSGGECRELEPALSPHIHCGAFHGGWYFVTDPERTVKSIAEHVVRGGGEI
                     IADDVVRIERDGTRAKALVLKSGRNVMLDRLVICGGAYSHLMAKQLGEKVLLEAERGY
                     HMVLPNSGVKLSRSLTYARTPGAATPMDMGLRLAGSDEFAGLEAPENWARADALWKIF
                     KQLLPGLNEPDSATTRWMGRRPGTPDSLPVIGPSKTVSNVWYGFGHSHMGLTWGPTTG
                     RLISELITGARSNIDLAPFRVDRF"
     gene            3593..>3796
                     /locus_tag="PKV23_03390"
     CDS             3593..>3796
                     /locus_tag="PKV23_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015667316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HQX84510.1"
                     /translation="MTLAILTAAIPATSFAADRIVVALGASHTFGKGVSRGQDYPAQL
                     QNLLSQKGNEAQVINAGINGQTTG"
BASE COUNT          731 a         1102 c         1195 g          768 t
ORIGIN      
        1 cggtatgacg gccgtcactt cgatttccac aacccattcg ggccgggcca gcgcggtgat
       61 gaccaggccg gttgaacagg gaaagacgcc cttcagccat ttgcccatct cgcggtagac
      121 atcctggcgg tagcggatat cggtgagata gacgacaatg cggcagatgt gatccatgtt
      181 gccgcccgct tcttccatca gcagcttgat gttggccatg gtcttggcgg tttgcgccgc
      241 tgcgtcgccg acgtgaagcg attcacgcgt gtcgagatcc tgcgagacct ggccacgcac
      301 gaataccata gtgccacggg ccacgacggc ctggcagagg tcattgtcga gcttctgctc
      361 gggataggtt tccttggtgt tgaagggacg gattctcttg tgtgcggtca ttattcggct
      421 gcctgttttt gcttgcccca aatgcggggg ctcatcccac cagccttctg agcgaatttc
      481 aaggtgctgt tccaatcgaa agtccggtag acggcagccg actgggcgaa gggcggggcc
      541 tgggcgaaca actggaaggt caggcggtgg ccggcgtaat ccgaattgag gagatcgcgg
      601 gccagcacca gcagcttgcg ccggtcgtcg gccagccatt cgtcattgat ggtgtaatac
      661 ttgtccatcc actttttggt ttcgggatgc tcgaagacgg cggcatcggg ggtgacgcaa
      721 atctgcccgc cgcagagttc gcgggcgatg tgcatcattc tgggaaggtt gttaagcgca
      781 tggcaacggc ccgacatcag caatgactgg ttgggcataa gcaggccatt ggggcttttc
      841 tcggccatga caatcgacgc ggtgagatgg gcgttgatac cctcgcgcca gacggcgagc
      901 tcgcttagtt tttcctgcac ggcctgctgc ttgtcgaggc cggtctgcct ggcgttccac
      961 agggcggcac cgatgatcag gtcggcatag gacagggtgc gctgcacgaa aggaaaagcg
     1021 gagtaacggt gcagcgtctg gcggatgaag gcggcggcgc gggtgtgctg gtagaacagg
     1081 atgtcctccc agggaatgag gacattgtcg agaatgacga tggtatccac ctcatcgaac
     1141 ttattggaga gcggatagtc acgttccggc gcgcggccgg tgaagccggt gcggcagatg
     1201 tgcttcacgc cgggagcgtt catcttcacg atgcagccca gggcatattc ggaaagcttg
     1261 gcatcgcccc agttggcgat ggtcggtttc aggaaggcct gattggcgta ggcggcggcg
     1321 gtttcatatt tcgcgccgcg aatgatgatg ccggcatcgg tttccttcac cacatggacc
     1381 agcatgtccg ggtcctgttc ctggggcggc tttgaacggt cgcccttggg atcggtattg
     1441 gccgagacat ggaaggggtc atgggcaatc gactcgtgaa tatggcgctc gatgttggcg
     1501 gcaaagcgcg gatcgatctc gttcaggatg tccttgccat cccagagcga ccacatttca
     1561 cccacggttt catcgcccac ccgggtgacg acgccgccca cttcatacat caccgcgtca
     1621 acggccttgc gcttgtcggt ccagtcctgc tgggcgcgcg gcaggcggtt gccgatggcg
     1681 aagcgttcgc ccttttcctc ataggtcatg acttcgcggt gggcggcctc atgctgcatg
     1741 tcgtatatgc gggccctgat atcgaccacg ggcttgaaca tgggatggcg cgtcacgtcc
     1801 ctgacgcgct cgccgttgat ccacacctca cggccatccc tgagggattc gcggtattgt
     1861 tcgcctgtcc tgatcatctc aattccccac aatgcggcaa tgcacccggt gaagatacga
     1921 tggaaaccag catcatggga acactcgatt tcgcagttaa aacgcggtgc tgcaccggaa
     1981 agaaccggcc ggccgggtga gccctcctcc cagtggggga aggcacaggg ctctggcgat
     2041 tgacacagga caacggcacg ggtgattggc cgatacaccc gggcccacgg ggattccacc
     2101 caggatggcg aacaattcgc catacgctcc attgccgggt aaattgagga cggcgttatt
     2161 gccatagcag ccctgaaaag cctgcggccg acgcggctga tctcgacctt tgaggaactc
     2221 tgtgtaagct tcttcaggaa aatccatccg aagagcacct gatttccatg tcccacccac
     2281 acgcgaccat catcggcgct ggcatagtcg gcgtttccag cgccgcattc ctgcagcggg
     2341 cgggataccg cgtgacgctc atcgaccggt tgccgccggg agaaggctgt tctttcggca
     2401 atgcgggcgg cctggcattt tgcgaaatcg tgccgcccat ccatccgcgc atcctgctca
     2461 agatcccggg ctggctcatg gatccgctgg ggccgctcac catccgctgg tcctacctgc
     2521 cgaaagccct tccctggctc ctggcggcag ggcgcaatgc catgccggcg cgggttgcgg
     2581 cgatcaccga agctcgcgcc aacctggcgc tgcgggtggt ggcggatttt gaaaccctgc
     2641 tccgcgatgc cggcagcgct gaattgctga aatacgaaga cacgctgcgg ctttacgaca
     2701 atgaacagca gttcgcggcg gaggccaacg aacgcgaagt caagaaagcc tatggctttg
     2761 agatgaagcg gctgagcggc ggggaatgcc gggaactgga gccagcactt tcaccccata
     2821 tccattgcgg ggcattccat ggcggctggt attttgtcac cgatccggaa cgcacggtga
     2881 aatccatcgc cgaacatgtg gtgcgcgggg gcggcgaaat catcgctgat gatgtggtgc
     2941 ggatcgagcg cgacggcacc cgggcgaaag cgctggtcct gaaatcagga cgtaatgtca
     3001 tgctcgacag gctggtgatc tgcggcgggg cctattcgca tctgatggcg aaacaactcg
     3061 gggaaaaggt tctgctcgaa gcagagcgcg gctaccacat ggttttgccg aactccgggg
     3121 taaagctttc gcgttcgctc acctatgcgc gaactccggg tgcggcaaca cccatggaca
     3181 tggggctgcg gctcgcgggc tccgatgaat tcgccgggct cgaggcgccg gaaaactggg
     3241 cccgggcgga cgccttgtgg aaaatcttca aacagcttct gcccgggctg aatgaacccg
     3301 attccgccac aacgcggtgg atggggcgca ggcccggcac gcccgattcc ctgccggtca
     3361 ttggcccgtc aaagacggtt tccaatgtct ggtatggttt tggccacagc cacatggggc
     3421 tgacctgggg gcccaccacc ggccgcctga tcagcgaatt gattaccggc gcgcggagca
     3481 atatcgatct tgcgccattc cgcgttgacc ggttctaaac aggagattgt ctccaatgtt
     3541 ccaagcacat cggccccgac acattgctgg caggctcagc cggtttctct ttgtgacact
     3601 ggccatcttg acggctgcaa ttcccgcgac cagttttgca gccgacagga ttgttgtcgc
     3661 ccttggcgcc agccatacat tcggaaaagg tgtcagccgc ggccaggact atcctgcaca
     3721 actccaaaat ctcctcagcc aaaagggcaa tgaggcgcag gtgatcaacg ccggcatcaa
     3781 tgggcagacg acgggc
//
LOCUS       DAORVN010000119         3777 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_19513_length_3777_cov_3.077915, whole genome shotgun sequence.
ACCESSION   DAORVN010000119 DAORVN010000000
VERSION     DAORVN010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3777)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3777)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3777
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19513_length_3777_cov_3.077915"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="PKV23_03395"
     CDS             <1..253
                     /locus_tag="PKV23_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014892474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HQX84511.1"
                     /translation="LSFRRGSKEERKFWQRTLEDGNQTEDDLLYAQKLMARHRAIDDT
                     IDRANHYGDIARDALAIFPDSAWKSSLLEAVDFCIIRAY"
     gene            complement(247..1098)
                     /locus_tag="PKV23_03400"
     CDS             complement(247..1098)
                     /locus_tag="PKV23_03400"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010971024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HQX84512.1"
                     /translation="MLVRERAHAKINLALHVTGRRQDGYHELDSIVAFADVADVLTIA
                     PAGAVSLSISGPFAGDLPGDGENSVTSAWTLLSEYSRQKGAPITPVNFQLEKNLPVAS
                     GIGGGSADAAAALRGLIRHFGLGISPDELSELALRLGADVPVCLFQQSSRMRGIGEII
                     APVSLELPSAMVLVNPLIPAPTSKVFSSLNLQPGQSFGAGIVNLLDIESWRNDLTAPA
                     VTLVPEIAEVIGSLNFQPEIICSRMSGSGATCFGLFESLGAAQTAAESITQKHPPWWV
                     VATTLLQ"
     gene            complement(1103..2827)
                     /locus_tag="PKV23_03405"
     CDS             complement(1103..2827)
                     /locus_tag="PKV23_03405"
                     /inference="COORDINATES: protein motif:HMM:NF024821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQX84513.1"
                     /translation="MLRFALPLASAAGFMMLVAAVPARAEFSFDEVSSLSGNYLAGRT
                     AGKERDNEIAADYLSKALIEDPDNPLLIERLFLLEVSSGNLPDSEELATKVLSFNSQH
                     RMARIVLGLRDFRIRHYADARRNFSEASYTPVGELTSALLSAWAFAGEGDLNDALATL
                     DKLDGNDSFANFQSYHSALIADYLSSAIRAEASYKKAYEQAGTSLRVVQAYGNFLERH
                     NRRDEAIKIYQAFIEGGDGNILIELALKRAQEGKPPLPFIATPGAGAGEALFSLAAAM
                     TDEQSIDVALLYAQLALSFNGDRPVVLTLLGDIYVDTRQYQNAISAYEQTPPGSALRT
                     NADTEIAINLQRLERTKEAQEKLKEIIAREPGNYLALVTLGNVYRSNEEFKSASDTYS
                     TALALVSKLEKEHWRVLYYRGISYERQKLWDKAEADFRQALALSPEEPLVLNYLGYSM
                     IEKKMNLNEALGMVKKAVELKPNDGYIVDSLGWAYFQLGDYEEATIHIERAVDLNPSD
                     PIIGEHLGDAYWRVGRTLEAKFQWQHAKDNDPQPEDLKRIEEKLLNGLPDEPVSKPAE
                     NHTDQTNG"
     gene            complement(2965..>3777)
                     /locus_tag="PKV23_03410"
     CDS             complement(2965..>3777)
                     /locus_tag="PKV23_03410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX84514.1"
                     /translation="SSTSYGTYANDTWNGSYIPESGTPSEVAGMTTTQKNDTSTAGLI
                     YRQQNQAKYVNRNIGSESTTSATNGPWKGCAKTPIVPLTYNRANVEAGITAMAAAGNT
                     LIAEGVAWGWRVLSPGEPFTKVEASTSFPADTISTYNHPRWLKIMVIMTDGDNDISAG
                     VDTLNGTTYTAYGRGKETLADNRFGTTNSSEIMGKLDDAMSEACTKAKAEGIELYVTS
                     FGSGVSTATRAKLQACATDADNYQHASSSADLAAFFNHIGQDVLNKSIYVSK"
BASE COUNT          755 a         1038 c         1200 g          784 t
ORIGIN      
        1 gttatctttc cgccgtggtt caaaggaaga aagaaaattc tggcagcgca cgctggaaga
       61 cggaaaccag acggaagacg acctgctcta tgcgcaaaag ctcatggcgc ggcaccgggc
      121 cattgatgac accatcgaca gggccaacca ctacggcgac attgcgcgtg acgcccttgc
      181 catattcccg gacagcgcct ggaagtcatc cctgctcgag gcggtggatt tctgcatcat
      241 ccgcgcttac tgaagcagcg tcgtagcgac aacccaccag ggggggtgtt tttgcgtgat
      301 ggactctgca gcggtttggg ccgcgccgag gctttcaaac agaccaaaac aggtggcgcc
      361 cgagcccgac atgcgcgaac agatgatctc gggctggaaa ttcagactgc cgatcacctc
      421 ggcgatttca gggacaagag tgaccgccgg cgccgtcaga tcattgcgcc agctctcgat
      481 gtcgagaagg ttgacgatcc ctgccccgaa ggactggcca ggctgcaggt tcaaggatga
      541 aaacaccttt gaggttggtg ccggaatgag gggattgacc agcaccatcg ccgagggaag
      601 ttcgagcgat acaggcgcaa tgatctcgcc gatgccacgc atgcggctgg attgctgaaa
      661 caaacagacc ggcacatcgg cgccgaggcg cagcgccagt tcgctcaatt catccggcga
      721 tattccaagc ccaaaatgcc ggatcaggcc gcgaagtgcg gctgccgcat cggcagagcc
      781 gccgccaatg cctgacgcaa cgggaagatt tttctcaagc tgaaaattga ccggggtgat
      841 gggagcgccc ttctgccttg aatactcgct cagcagggtc caggcggagg tgacactgtt
      901 ctcgccatcg ccaggaagat caccggcaaa tggcccggaa atgctgaggg aaaccgcgcc
      961 cgccggggca atggtcagga catccgccac gtcggcgaag gccacgatgg aatcaagttc
     1021 gtgatagccg tcctggcgcc gccctgtcac atgcaatgcg aggtttatct tggcgtgggc
     1081 ccgctcacgc accagcatgg ggttatccgt tggtctggtc cgtgtggttt tcagcaggct
     1141 tgctgaccgg ttcgtcaggc aggccgttca gcagtttttc ctcgatgcgt ttcaggtcct
     1201 cgggctgggg atcattgtcc ttggcgtgct gccactgaaa ctttgcttcc agggtgcggc
     1261 cgacgcgcca ataggcgtca cccagatgtt cgccgataat cgggtccgac ggattcagat
     1321 ccacggcgcg ctcgatgtgg attgtcgctt cctcgtagtc gccgagctgg aaataggccc
     1381 agcccaggct gtcaacgata tagccgtcat tgggcttcag ctcgacggcc ttcttcacca
     1441 tgcctaaggc ttcgttgagg ttcatcttct tctcgatcat cgagtagcca aggtagttca
     1501 gcaccagcgg ctcttcgggc gaaagcgcca gggcctggcg gaaatcagct tcggccttgt
     1561 cccagagctt ctggcgttca taggaaatgc cgcgatagta aagcacgcgc cagtgctctt
     1621 tctccagctt cgagacgagc gccagggcgg tggaataggt gtcggaggcg gatttgaact
     1681 cttcgttgct gcggtagaca ttgcccaggg tgaccagggc cagataattc ccgggctcgc
     1741 gcgcgatgat ttccttgagc ttctcctggg cttccttggt gcgctccagg cgctgcaggt
     1801 tgatcgcgat ctcggtatcg gcgttggtgc gcagggccga gcccggcggg gtttgctcat
     1861 aggcgctgat ggcgttctgg tactggcgcg tatccacata gatatcgcca agcagggtga
     1921 ggaccaccgg cctgtcgccg ttgaacgaaa gggcaagctg ggcatacagc aaggcgacgt
     1981 cgatgctctg ctcgtcagtc atggccgcgg caagagaaaa cagggcttca ccggcgccgg
     2041 ctcccggcgt tgctatgaag ggcagcggcg gctttccctc ctgggcgcgc ttcagggcaa
     2101 gttcaatcaa tatgttgcca tcgccgcctt caatgaatgc ctgatagatc ttgattgcct
     2161 cgtcgcggcg gttgtggcgt tcgaggaagt ttccgtaggc ctggaccacc cgcagggagg
     2221 ttcccgcctg ctcataggct ttcttgtagg acgcctcggc gcgaatggcc gaggagagat
     2281 agtcggcgat cagggcactg tggtaggatt ggaaattggc gaaggaatca ttgccatcga
     2341 gcttgtcaag cgtggcgagg gcatcgttca ggtcgccttc gccggcaaag gcccaggccg
     2401 acaaaagcgc cgaggtcagt tcgccaacgg gggtgtagga ggcttcgctg aaattccggc
     2461 gggcgtcggc gtagtggcgg attcgaaaat ccctgaggcc cagaacgatg cgggccatgc
     2521 gatgctggct gttgaatgac agcaccttgg tggccagctc ttccgagtca ggcagattgc
     2581 cagacgagac ttcgagcaga aacagccttt cgatgagaag cggattgtca ggatcctcga
     2641 tcagggcctt gctgagataa tcggcggcta tctcgttgtc gcgctctttg cccgccgtgc
     2701 gcccggcgag gtagttgccg gacaggctgg acacctcgtc gaacgaaaac tcggcacggg
     2761 caggaaccgc cgcgacaagc atcataaagc cagccgcgct ggcgagaggc agggcaaatc
     2821 tgagcattgg catccgtctc acgaaaaaaa agcggccact tcagccgaaa agtgattcat
     2881 cggcataaaa tggggttttt tcaggataac cgcaatagca aaacggcggg cctgggcccg
     2941 ccgccggttt gccgcaaaag cggcttattt gctgacgtaa atcgacttgt tgagcacgtc
     3001 ctggccgatg tggttgaaga aggcggccag gtcggcgctg gaggaggcat gctggtagtt
     3061 gtcggcatcg gtggcgcagg cctgcagttt ggcccgagtc gcggtggaaa caccggaacc
     3121 aaaggacgtc acatagagct cgatgccttc agccttggcc ttggtgcagg cttcagacat
     3181 tgcgtcgtcg agtttgccca tgatttccga actgttggtc gtgccgaagc ggttgtcagc
     3241 aagggtttcc ttgccgcggc cataggccgt gtaagtcgtg ccgttcaatg tatcgacgcc
     3301 ggcgctgata tcgttgtcac cgtcggtcat gataaccatg atttttagcc agcgcggatg
     3361 attataggtg gaaatcgtgt cggccgggaa cgaggtcgag gcttccacct tggtgaaagg
     3421 ttcgccgggc gagagaacac gccaacccca ggccacgcct tcagcaatga gcgtgttgcc
     3481 ggcggcggcc atggccgtga tgccggcttc aacgttggcc cgattgtaag tcagggggac
     3541 gatcggggtc ttggcgcagc ctttccacgg gccatttgtg gcggaggtcg tagattcgga
     3601 gccgatgttc cggttcacgt atttggcctg gttctgctgg cgatagataa ggccggcggt
     3661 gctggtatca ttcttttgag tcgtggtcat accggcaact tcgctcggcg ttccgctttc
     3721 aggaatatag gagccgttcc aggtatcgtt cgcgtaagta ccgtaagaag ttgaact
//
LOCUS       DAORVN010000120         3767 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_19593_length_3767_cov_3.076778, whole genome shotgun sequence.
ACCESSION   DAORVN010000120 DAORVN010000000
VERSION     DAORVN010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3767
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19593_length_3767_cov_3.076778"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2149)
                     /gene="pnp"
                     /locus_tag="PKV23_03415"
     CDS             complement(<1..2149)
                     /gene="pnp"
                     /locus_tag="PKV23_03415"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011313367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HQX84515.1"
                     /translation="MFKIDVEELEWAGKPLRIETGRMARQADGAVVVTYGETTVLATA
                     VAARSEKPGIDFFPLTVNYQEKTYAAGKIPGGYFKREGRPTEKETLVSRLIDRPIRPL
                     FPEGFRNETQVIATVLSYDLENDSDIVALIGASAALTISGIPFMGPVGGARVGFIDGE
                     FVLNPTQEQMKTSDLDLVVAGTSDAVLMVESEAKELSEEQMLGAVMFGHAGFQPVIDA
                     IIRLAERSSREPRDFVAPDNEKLYKAVKKSVEKDVRAAYAIPKKEERVEAIAKAKDKV
                     VAALVDAEKDTSPTPQKVAEAFKHLEKDVVRNSILDTGLRIDGRDTKTVRQIIAEASV
                     LPRTHGSSLFTRGETQALVVTTLGTGEDEQYVDSLDGTYKAKFMLHYNFPPFSVGEVG
                     RMGGAGRREIGHGKLAWRAINPMLPPADQFPYTIRVVSEITESNGSSSMATVCGSSLA
                     LMDAGVPLKSPVAGIAMGLIKEDERFAVLSDILGDEDHLGDMDFKVAGTSNGVTALQM
                     DIKITGITEEIMKVALWQAKDGRLHILGKMAEALENARPGLGEHAPRIEVIKIPTDKI
                     REVIGTGGKVIREIVEKTGAKVNIDDDGTVKVASADGESIKAAIKWIKSIASDPELGQ
                     IYDGTVVKVMEFGAFVNFFGAKDGLVHISQLASARVQKTSDVVKEGDKVKVKLLGFDD
                     RGKTRLSMKAVDQVTGEDLEAGQKAEAPAREAAG"
     gene            complement(2308..2577)
                     /gene="rpsO"
                     /locus_tag="PKV23_03420"
     CDS             complement(2308..2577)
                     /gene="rpsO"
                     /locus_tag="PKV23_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011995947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HQX84516.1"
                     /translation="MTMTAERKSALMKDYGTKPGDTGSPEVQVALLTERINGLTDHFK
                     THKKDNHSRRGLLKMVSLRRGLLDFLKSKDEGRYQALIKRLEIRR"
     gene            complement(2597..>2968)
                     /locus_tag="PKV23_03425"
     CDS             complement(2597..>2968)
                     /locus_tag="PKV23_03425"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006608908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HQX84517.1"
                     /translation="ARDMGRKLGCLGHVSMLRRIAVGPFVEADMIPLETLAEMSHKPL
                     GNSAENGALRPLETVLDGIPALALKDAEAQRLKQGQSILLRGANAPVACDAVLVTWDS
                     KPLGICSIEQGALKPKRLFNL"
     assembly_gap    2969..2978
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2979..3520)
                     /gene="truB"
                     /locus_tag="PKV23_03430"
     CDS             complement(<2979..3520)
                     /gene="truB"
                     /locus_tag="PKV23_03430"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018319414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HQX84518.1"
                     /translation="MKKRGQPVHGWVVLDKPLGMTSTQAVGKIRRLFDAEKAGHGGTL
                     DPLATGMLPIALGEATKTVQWAMDGRKVYRFEVTWGAERTTDDLEGEITAQSDLRPTP
                     INIESILTDFQGVILQAPPAFSAIKIDGERAYDLARAGETVEIAERPVTIEKLVLVGC
                     PDDNHAIFEVSCGKGTYVRSL"
     gene            complement(3517..>3767)
                     /locus_tag="PKV23_03435"
     CDS             complement(3517..>3767)
                     /locus_tag="PKV23_03435"
                     /inference="COORDINATES: protein motif:HMM:NF014130.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0030490 - maturation of SSU-rRNA [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribosome-binding factor A"
                     /protein_id="HQX84519.1"
                     /translation="MSPDLRIATAYVRGLINSQDKLVLAALERNKKYVRGLVAPRIDV
                     KYMPEIRFRIDTSLEYAAKIDELLADPKVVSDLKHNEK"
BASE COUNT          704 a         1162 c         1067 g          824 t
ORIGIN      
        1 cgccggcggc ttcgcgcgcc ggagcttcgg ccttctggcc ggcttcgagg tcctcgccgg
       61 tcacctggtc gaccgccttc atcgacagac gcgtcttgcc gcgatcgtcg aagccgagca
      121 gcttgacctt gaccttgtcg ccttccttga cgacatcgga ggtcttctgc acgcgggccg
      181 atgcgagctg gctgatgtgg acgaggccgt ccttggcgcc gaagaagttc acgaacgcgc
      241 cgaactccat caccttgacc acggtgccgt catagatctg gccgagctcc ggatcggaag
      301 cgatcgactt gatccacttg atcgccgcct tgatcgactc gccatcggcg gacgcgacct
      361 tgacggtgcc gtcgtcgtcg atgttgacct tggcgccggt cttttccacg atttcgcgga
      421 tcaccttgcc gccggtgccg atcacttcac ggatcttgtc ggtcgggatc ttgatcactt
      481 cgatgcgcgg cgcatgctcg ccaaggcccg gacgggcgtt ttcaagcgcc tcggccatct
      541 tgccaaggat atgcaagcgg ccatccttcg cctgccacaa ggcaaccttc atgatctcct
      601 cggtgatgcc ggtgatcttg atgtccatct gcagtgcggt cacgccattg ctggtgcccg
      661 ccaccttgaa gtccatgtcg ccgagatgat cctcgtcgcc gagaatatcc gagagcaccg
      721 caaagcgctc gtcttccttg atcaggccca tggcaatgcc ggctaccggt gatttcagcg
      781 gaacgcccgc atccatcagc gccagcgacg aaccgcacac ggttgccatc gatgaggagc
      841 cgttggattc agtgatctcc gacaccacgc gaatggtgta ggggaactgg tcggccggcg
      901 gcagcatcgg attgatcgcc cgccaggcca gcttgccatg gccgatttca cggcggccgg
      961 cgccgcccat gcggcccact tcacctaccg agaagggagg gaagttgtaa tgcagcatga
     1021 acttggcctt gtaggtgccg tcgagcgagt cgacatattg ctcgtcttcg ccggtgccca
     1081 gtgtcgttac aacaagcgcc tgggtttcac cgcgggtaaa cagcgatgaa ccgtgggtgc
     1141 gcggaagcac cgaagcttcg gcaatgatct ggcgcaccgt cttggtgtcg cggccatcga
     1201 tgcgaagtcc cgtgtcaaga atgctgttgc gtacaacatc cttttcgaga tgcttgaagg
     1261 cttcagccac tttctggggt gtcggcgagg tatctttttc cgcgtcaacg agggcggcca
     1321 caaccttgtc cttggccttg gcaattgctt ccacccgctc ttccttcttc ggaatggcgt
     1381 aggcggcgcg cacgtctttc tcgacggact tcttcaccgc cttgtaaagc ttctcattgt
     1441 ccggagccac gaagtcgcgg ggttcgcgcg atgagcgttc ggccaggcgg attatggcgt
     1501 cgatgacggg ctggaagccc gcatggccaa acatcacggc gccaagcatc tgctcttccg
     1561 aaagctcctt ggcttccgat tcaaccatca gaaccgcgtc tgatgtgccg gcaaccacaa
     1621 ggtcaaggtc agaagttttc atctgctcct gcgtcgggtt caaaacgaac tcgccatcga
     1681 tgaagccgac gcgggccccg ccaaccgggc ccatgaaggg aatgccggaa atggtgagtg
     1741 cggcggatgc accgatgagg gccacgatat cgctgtcgtt ctccaggtcg taggagagca
     1801 ccgtggcgat cacctgggtt tcattgcgga aaccttcagg gaaaagcggg cggatggggc
     1861 ggtcgataag gcgggaaacc agtgtttcct tttccgtggg ccggccttca cgcttgaagt
     1921 agccgccggg aatcttgcct gcggcatagg tcttttcctg gtagttcacg gtgagcggga
     1981 agaaatcaat cccgggcttt tccgagcgcg cggcaactgc cgtggcaaga accgtcgtct
     2041 cgccgtaggt tacgacaacc gcgccgtctg cctggcgcgc catgcggccg gtttcaatgc
     2101 gcaagggctt tccggcccat tccagttctt caacatctat cttaaacatg taacattcct
     2161 tattgctctt cgccgccact tccgttcacc tgaccggacg gcatccccat tgagcgatca
     2221 aaacctggcc ccattgcccg gcttgtgaaa aggggacgcg ggcggcaatt cggtgatccg
     2281 cccgctcata caatcgtcag ccgctgttta gcggcggatt tcgagacgct tgatcagggc
     2341 ctggtaacgg ccctcatcct tcgacttcaa gaagtcgaga agtccacggc gcaaactcac
     2401 catcttcaac aaaccgcggc gtgagtgatt gtccttcttg tgggttttga aatggtcggt
     2461 cagaccattg atgcgctctg tcaaaagagc aacctgcact tcaggcgagc ccgtgtcacc
     2521 gggcttggtt ccatagtctt tcatgagcgc ggatttacgc tcggcagtca tcgtcattcg
     2581 gccatctcca ggatgtctaa aggttaaaca ggcgcttcgg cttgagcgct ccctgttcga
     2641 tgctgcagat gccgaggggt tttgaatccc aggtcaccag aaccgcatcg catgcaacgg
     2701 gagcgttcgc acctctgagc aggattgact gaccctgctt caggcgctgg gcttccgcgt
     2761 ccttcaaggc cagtgccggg atgccgtcca gcacggtctc gagggggcgg agagccccgt
     2821 tttcggcact attgccgagg ggcttatggc tcatttccgc gagggtttcc agcggaatca
     2881 tgtcagcttc cacaaatggc ccaaccgcaa tccgccgcaa catggaaaca tggcccaggc
     2941 agcccagttt ccggcccatg tcacgggcnn nnnnnnnnag cgagcgcaca taggtgccct
     3001 tgccgcagct cacctcaaaa atcgcatgat tgtcgtcggg gcagcccacc agaaccagct
     3061 tttcaatggt taccggccgt tcggcgatct cgacggtctc gcccgcccgg gccaggtcat
     3121 aagcccgctc cccgtcaatc ttgatggctg aaaaagccgg cggggcctgc agaataacgc
     3181 cctggaagtc agttagtatc gattcgatat taattggtgt cggacgcaaa tcagattgag
     3241 cggtaatctc gccttccaaa tcgtcggtcg tgcgctccgc cccccaggtc acttcaaagc
     3301 ggtagacttt ccgcccatcc atggcccact gcacggtttt ggtggcttct cccagagcaa
     3361 tcggcagcat tcccgtagcg agtggatcca atgtcccgcc gtgcccagcc ttttccgcat
     3421 cgaacagccg ccggattttg ccaaccgcct gtgtcgaggt catgcccaag ggcttgtcca
     3481 gcaccaccca gccatgaacc ggctgcccgc gctttttcat ttttcgttat gtttgaggtc
     3541 gctgacaacc tttggatcgg ccaggagctc atcgattttg gcagcgtact ccaaagacgt
     3601 atcgatacga aaccttattt ccggcatgta ttttacgtca atgcgcggcg ccacaagccc
     3661 gcggacatac ttcttgttcc gctctaaggc tgccagcacc agcttgtcct gactgttgat
     3721 caggccccgc acataggccg tggcaatccg caaatccggt gacatct
//
LOCUS       DAORVN010000121         3759 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_19667_length_3759_cov_3.410907, whole genome shotgun sequence.
ACCESSION   DAORVN010000121 DAORVN010000000
VERSION     DAORVN010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3759)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3759)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3759
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19667_length_3759_cov_3.410907"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..930)
                     /locus_tag="PKV23_03440"
     CDS             complement(<1..930)
                     /locus_tag="PKV23_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013167787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HQX84520.1"
                     /translation="MSNKPYRLAQGGSRIDRTKKLHFSFDGKQLNGFAGDTLASAVMA
                     NGTKLFGRSFKYHRPRGVVGLGSEEMNALVGVGDGSRHEPNLRATQVELFDGLQAVSQ
                     NRWPSLAFDVSSLNSSFSRFIPGGFYYKTFMWPQRFWKHVYEPIIRKAAGLGHAPEGR
                     DPDSYENIHVHCDVLVVGGGVAGLAAAESAAATGARVVVADENPQFGGVADISAGTLD
                     SMPQATWIAEKVAALAAAENVRLLPRATVVGHWHHNYLMLFERVADHDPALLREGAPR
                     HRLWKVRAKQVILATGAIERPIAFANNDRPGIML"
     gene            complement(927..1280)
                     /locus_tag="PKV23_03445"
     CDS             complement(927..1280)
                     /locus_tag="PKV23_03445"
                     /inference="COORDINATES: protein motif:HMM:NF016179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008115 - sarcosine oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0046653 - tetrahydrofolate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit delta"
                     /protein_id="HQX84521.1"
                     /translation="MLVVHCPVCGAEGDETDFHGGGQAHVRRPATHDPANVSDEAQRD
                     YLFIRHNPKGLHFERWRCDRGCGKWFHAARDTVTMEFKAFYGISELPPREVMEQATGE
                     WATYFKDRLKAEAKS"
     gene            complement(1292..2545)
                     /locus_tag="PKV23_03450"
     CDS             complement(1292..2545)
                     /locus_tag="PKV23_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020812334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008115 - sarcosine oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0046653 - tetrahydrofolate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit beta family protein"
                     /protein_id="HQX84522.1"
                     /translation="MKSRYSIFSLARNAMNYHKDWERAWRSPQPKAEYDAVIVGAGGH
                     GLATAYYLAKNHKMTNIAILEKGWLGGGNTGRNTTIIRSNYLQDSSIAIYELARSLYE
                     GLSQELNYNVMFSPRGVLMLCQTEHELRAFKRTAHANRLAGLDCRMVSPAEVKEIVPI
                     INDDPNCRYPVLGAYYQPRGGTARHDAVAWGYARGADALGVDIIQNCEVTGITREGGK
                     VVGLETSRGSIKTKKVGVVTAGNVSTLMQTAGVRMPIESLCLQALVSEPIKPVIDCVV
                     MANTVHGYVSQSDKGELVIGGGTDPYNNYSQRGSFPSLEHTVTALVETFPIISRLRML
                     RMWGGIVDMTGDRSPIISKTDVEGLYVNCGWGTGGFKAIPGSGYVFADTIAHDRPHAI
                     AEPFGLNRFAEGRLIDESVAAAVAH"
     gene            2702..3682
                     /gene="ccmI"
                     /locus_tag="PKV23_03455"
     CDS             2702..3682
                     /gene="ccmI"
                     /locus_tag="PKV23_03455"
                     /inference="COORDINATES: protein motif:HMM:TIGR03142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcmI"
                     /protein_id="HQX84523.1"
                     /translation="MIWLIFAGMTAAVVAALLVPFMRNSADTLSPDSEFDRAIYRDQL
                     LELDRDKARGLIGETEAQAARNEISRRLLQVAKPEALAAGNRYALMAVLLVPLMAMPI
                     YAKYGSPRMPDVPLQERLKGAIANQDFEALVATVEAHLAKSPNDVEGWKVLAPAYKRE
                     RRWRDAAEAYANVLRLAPPTSDAIADYGEMLVFANEGMVTDEAERAFVEALKLEATNE
                     RAKYYYDMALKQEGKAPALSDEQIAAGQAMPAQDQMAMIAGMMDGLEQKLGRDSRDLE
                     GWKRLIRARHISNQIDKAKASLDLAMNIFKDEPETLDALRALAKELGIAE"
BASE COUNT          773 a         1169 c         1087 g          730 t
ORIGIN      
        1 cagcatgatg ccgggccggt cattgttggc gaaggcgatg gggcgttcaa tggccccggt
       61 ggcaaggatc acctgcttgg cgcggacctt ccacagcctg tggcgtggcg cgccttcgcg
      121 aagcagcgcc ggatcatggt cggccacacg ctcaaacagc atcaggtaat tatggtgcca
      181 atggcccacg accgtggcgc ggggcaggag acgcacattc tccgccgccg caagcgcggc
      241 gaccttctcg gcaatccagg tcgcttgtgg catgctatca agcgtcccgg cggaaatgtc
      301 tgcaacaccg ccgaactgcg gattctcatc ggccacgaca acccttgcgc ccgttgcggc
      361 ggcggattcg gccgccgcca gtccggcaac cccgccgccc accaccagca catcgcaatg
      421 cacatgaatg ttttcgtatg agtcgggatc gcggccttcc ggcgcatggc caaggcctgc
      481 cgccttgcgg atgatcggct catacacatg cttccagaag cgctgcggcc acatgaaggt
      541 cttgtagtaa aagccgccgg gaatgaagcg cgagaagctg ctgttgagcg aactcacatc
      601 gaaggcaagc gatggccagc ggttctggct caccgcctgc agcccgtcga agagttccac
      661 ctgcgtggcg cgcaaattgg gctcgtggcg cgaaccatcg cccacgccga caagggcgtt
      721 catttcttct gagccgaggc ccaccacccc acgcgggcgg tgatacttga aactgcggcc
      781 gaaaagcttt gtgccattgg ccatcacggc tgaggccagc gtgtcaccgg caaaaccgtt
      841 gagctgcttg ccgtcaaaac tgaaatgcag cttcttcgtg cggtcaatgc gggagccgcc
      901 ctgggccagg cggtaaggct tgttgctcat gattttgcct ctgccttcag cctgtccttg
      961 aaataggtgg cccattcgcc cgtggcctgt tccatcacct cgcgcggcgg cagttcgctg
     1021 atgccgtaaa acgccttgaa ttccatggtc accgtgtcgc gcgccgcatg gaaccatttc
     1081 ccgcagcccc ggtcgcagcg ccagcgctcg aaatgcaggc ccttcggatt atgccggatg
     1141 aacaggtaat cgcgctgcgc ctcatcgctg acattcgccg gatcatgggt cgcaggccgg
     1201 cgcacatggg cctggccacc gccatgaaaa tccgtctcgt cgccttccgc gccacacact
     1261 gggcaatgaa caactaacat atcatctgct cctaatgcgc caccgctgcc gcaacacttt
     1321 cgtcaatcag gcggccttcc gcaaaacggt tgagcccgaa aggctcggcg attgcgtgcg
     1381 gccggtcatg ggcgatggtg tcagcaaaga catagcccga acccggaatg gccttgaagc
     1441 cgccggtgcc ccaaccgcaa ttcacataga ggccttccac atcggttttg gaaatgatcg
     1501 gtgaacggtc gcccgtcatg tcgacaatgc cgccccacat gcgcagcatg cgcagccgcg
     1561 aaatgatcgg gaaggtttcc accagcgccg tcaccgtgtg ctcgagggag gggaaggagc
     1621 cgcgctgcga atagttgttg tagggatccg tgccgccccc gatcaccagt tcgcccttgt
     1681 ccgactggct gacatagcca tgcaccgtat tggccatgac cacgcagtca atgaccggct
     1741 tgatcggttc agacaccagc gcctgcaggc agagactttc gatcggcatg cgcacgccgg
     1801 cagtctgcat caaggtcgag acattgcctg ccgtcaccac cccaactttc ttcgtcttga
     1861 tggagccacg cgatgtttca agtccaacga cctttccgcc ttcgcgggtg atgccggtga
     1921 cttcacagtt ctggataatg tcgacgccca gtgcatcggc cccccgggca tatccccagg
     1981 ccaccgcatc atgccgcgcc gtgccgccgc gcggctggta ataggcgccg aggacgggat
     2041 agcggcagtt cggatcatca ttgatgatcg gtacgatttc tttcacttcc gccggtgaaa
     2101 ccatccggca atccaatccc gccaggcgat tggcgtgcgc cgtgcgcttg aaggcgcgaa
     2161 gctcatgctc ggtctggcac agcatcaaca cgccacgggg cgagaacatc acattgtaat
     2221 tcagttcctg gctcagccct tcgtaaagcg agcgcgccag ttcataaatg gcaatcgacg
     2281 aatcctgcag gtaattggag cggataatgg tggtattgcg ccccgtattg ccgccgccca
     2341 gccagccctt ttccagaatg gcgatattcg tcattttatg gttcttggcg aggtaatagg
     2401 cggtggccag gccatgcccg ccggccccca cgatcaccgc gtcatactcc gccttaggct
     2461 gtggcgaacg ccaggcccgc tcccaatcct tgtggtaatt catggcgttg cgggcgagcg
     2521 aaaagatcga atagcgggat ttcatcgggc ctatccttaa ttcgcctgac ttgtatcaaa
     2581 taggccccac aatcactaca gctttccgac accccattgc cgccatgcga caatgcgacg
     2641 tgacagatgc ttgcctatga attgtattgt aagtccacca tgggaaaacc ataaccgcat
     2701 gatgatttgg ctgatttttg caggcatgac ggcggcagtc gtggcagctt tgctggtgcc
     2761 tttcatgcgc aattcggcgg atacccttag tcccgattcg gagtttgatc gggcgatata
     2821 ccgcgaccag ctgctggaac ttgaccgcga caaggcccgc ggcctgattg gcgagacaga
     2881 ggcccaggca gcccgcaatg agatttccag gcgcttgctc caggtcgcaa agccggaggc
     2941 tcttgccgcc ggaaaccgct acgcccttat ggccgttctg ttggtgccgc ttatggccat
     3001 gcccatctac gccaagtatg gcagtccgcg gatgcccgat gttcccttgc aggaaagatt
     3061 gaaaggggcc attgcaaatc aggactttga agccttggtg gcaaccgttg aggcccatct
     3121 cgccaaatct cccaatgacg tcgagggctg gaaagtgctg gctccggctt acaagcgcga
     3181 aaggcgctgg cgcgatgcgg cggaggccta tgccaatgtc ttgcgtctcg ccccgccaac
     3241 ctctgacgca attgccgatt atggtgaaat gctggtcttc gccaatgagg gcatggtgac
     3301 cgatgaggcc gagcgcgcct ttgtcgaagc actgaaactc gaagcaacca acgaacgtgc
     3361 caaatactat tacgacatgg ccctgaaaca ggagggcaag gcccccgctt tgagcgatga
     3421 acagattgcc gcgggacagg ccatgccggc ccaggaccag atggcgatga ttgccggcat
     3481 gatggatggc ctggagcaaa agctgggcag ggacagccgc gacctcgaag gctggaagcg
     3541 cctgatccgc gcccggcata tctcaaacca aatcgacaag gccaaagcct cgctcgacct
     3601 cgccatgaac attttcaagg acgagcccga aacgctggat gccttgcgcg cccttgcaaa
     3661 ggaattgggg atagcagaat gacccgtaaa cagaagcgcc tggccgtaat cgccggtggc
     3721 cttgccattc tcggccttgc cgcaggcctc gacaagaac
//
LOCUS       DAORVN010000122         3752 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_19734_length_3752_cov_3.557479, whole genome shotgun sequence.
ACCESSION   DAORVN010000122 DAORVN010000000
VERSION     DAORVN010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3752)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3752)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3752
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19734_length_3752_cov_3.557479"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1293
                     /locus_tag="PKV23_03460"
     CDS             <1..1293
                     /locus_tag="PKV23_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009338484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HQX84524.1"
                     /translation="TMAPLFDLIVSHFNPPVIEEGPFRMLVTTIEANPFLGRVLTGRV
                     RSGSVKANQQVRALSQDGKVVEQGRISKVLAFRGLDRVPVDECFAGDIVAISGLTTST
                     VADTICDPTVNEPIKAQPIDPPTLTMTFRINDGPLAGKEGDKVQSRVIRERLLREAEG
                     NVALKVTASSTETDAFEVAGRGELQLGILIETMRREGFELTVGRPRVVFRTDEHGQKL
                     EPIEEVIVDVDEAYSGIVVQKLSERRAEMRDMRPSGAGRQRLVFHAPTRGLIGYQGEL
                     MSDSRGTAIMNRLFHAYAPYVGDIPGRHTGALLSNGDGEAVAYAIFNLQDRGPMFIEH
                     GTKVYAGMIIGEHTRGNDLELNVIKGKKLTNVRASGKDEAVVLTPPIKMSLEKALAYV
                     GDDELVEITPKSIRLRKIYLDANERKRMSRAKEAAEVA"
     gene            complement(1297..1905)
                     /locus_tag="PKV23_03465"
     CDS             complement(1297..1905)
                     /locus_tag="PKV23_03465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX84525.1"
                     /translation="MGKFAALIVLGLSASAAAAGEITSIYSDLDLKACEALELDSGED
                     NGEGGIWQCKGIPGFDVLYLEGDLRGFIAFGPEARSQCTSAQTFGAFNSPGPRIEWRM
                     ENGKPIATILRWFTDNGSGEEYAKQNWLVVSKLNGRDACRTALIDTKYPEANTVARAK
                     ADGPARRFNCEKDMPEVVSQRAITAGELMSDVPCPGGPYREE"
     gene            1948..2484
                     /gene="ppa"
                     /locus_tag="PKV23_03470"
     CDS             1948..2484
                     /gene="ppa"
                     /locus_tag="PKV23_03470"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009734779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HQX84526.1"
                     /translation="MRIDAIAAGRNPPKEINVIVEVPVGGEPIKYEMDKEAGTLVVDR
                     FLYTSMRYPGNYGFIPHTLSGDGDPVDVLIANQRGIIPGAVVACRPVGVLKMQDEAGG
                     DEKIIAVPVPRLTRRYENVFNYLDLPEITVQQIEHFFTHYKDLEAGKWVKVLGWGNAG
                     EAEKMITDGIARAKAAKK"
     gene            complement(2580..3275)
                     /locus_tag="PKV23_03475"
     CDS             complement(2580..3275)
                     /locus_tag="PKV23_03475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX84527.1"
                     /translation="MDWEHAIKRNSEVLAGIVETLFVMLGLVGEATVSRIPWPAYRAV
                     LRVLRPAESALRRLIVVAARGLVVEPPASRPGPAGAKRARRGGYKRSLSFQLFDPQPR
                     IMLPRRKRTPRAVPRIHFFNTDGEFITIGPPIRPAKAPARAKSADGMVNAARVIRRLE
                     ALEAALQDLPRQAKRLVRWRMREEKSENPSFKTPLRPGRPPGSRRRAVHLVDELLDEC
                     QWLAHRAAMPDTS"
     gene            complement(3367..3549)
                     /locus_tag="PKV23_03480"
     CDS             complement(3367..3549)
                     /locus_tag="PKV23_03480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX84528.1"
                     /translation="MDDHYADKPRILPRIAFVFNIVMARLRLFARKSVSGKPCNYLAQ
                     HPRVIIASLNSKNNIS"
BASE COUNT          756 a         1159 c         1126 g          711 t
ORIGIN      
        1 accatggcgc cgctgtttga tctcattgtc tcgcatttca atccgccggt gatcgaggaa
       61 ggccccttcc gcatgctggt caccaccatc gaagccaacc cgttcctcgg ccgggttctc
      121 accggccgcg tccgctccgg ctcggttaag gccaaccaac aggtgcgggc cctttcccag
      181 gacggcaagg ttgtcgagca gggccgcatt tccaaggttc tggccttccg tggcctcgac
      241 cgcgtgccgg tcgatgaatg cttcgccggt gacatcgttg ccatctccgg actgaccacc
      301 tcgacggttg ccgatacgat ttgcgatccc acggtgaacg agccgatcaa ggcccagccc
      361 atcgatccgc caaccctcac catgaccttc cgcatcaatg acggcccgct cgccggcaag
      421 gaaggcgaca aggtccagag ccgcgtcatc cgcgagcgcc tgctccgcga agccgaaggc
      481 aatgtggccc tcaaggtcac cgcctcatcc actgaaaccg acgccttcga agtggcgggc
      541 cgcggcgaat tgcagttggg catccttatc gaaaccatgc gccgcgaagg ctttgaactc
      601 accgtgggcc gcccccgcgt cgtcttccgc accgatgagc acggccagaa gctcgaaccc
      661 atcgaagaag tgattgtcga cgtcgacgag gcctattccg gcattgtggt gcagaagcta
      721 tcggaacgcc gcgccgaaat gcgcgacatg cggccctccg gcgccggccg ccagcgcctg
      781 gtgttccatg cccccacccg tggcctcatc ggctaccagg gcgaactcat gtccgacagc
      841 cgcggcaccg ccatcatgaa ccgcctgttc cacgcctatg cgccttacgt cggcgatatt
      901 ccgggccgcc atacgggagc actcctctcc aacggcgatg gcgaggccgt ggcctatgcc
      961 attttcaacc tgcaggaccg tggccccatg ttcatcgaac acggcaccaa ggtctatgcc
     1021 ggcatgatca tcggcgaaca cacccgtggc aatgatctgg aactcaatgt gatcaagggc
     1081 aagaagctca ccaacgtgcg cgcctccggc aaggatgaag cggtggtgct gaccccgccc
     1141 atcaagatgt cgctggaaaa ggcgcttgcc tatgtgggcg atgatgagct ggtggagatt
     1201 acgccgaaat ccatccggct ccgcaagatc tatctcgatg ccaacgagag aaagcgcatg
     1261 agccgcgcca aggaagctgc cgaagtcgcc taacccctat tcctcgcggt aaggcccgcc
     1321 cggacagggc acgtcggaca tgagttcgcc cgccgtgatg gcgcgctgcg aaaccacctc
     1381 cggcatatcc ttttcgcagt tgaagcggcg cgccgggcca tcggctttgg cgcgcgcgac
     1441 tgtattggcc tccggatatt tcgtgtcgat cagcgccgtg cggcaggcgt cccgcccgtt
     1501 gagcttgctg accaccagcc agttctgctt ggcatattct tcgcccgaac cattgtcggt
     1561 gaaccagcgc aggatggtgg caatgggctt gccgttttcc atgcgccatt caatcctggg
     1621 gccgggcgaa ttgaaggccc cgaaggtttg cgccgaagtg cactggctgc gcgcttccgg
     1681 gccgaaagcg ataaagccgc gcaagtcgcc ctcgagatag agaacgtcaa agcctggaat
     1741 gcccttgcac tgccagatgc cgccttcgcc attgtcttcg ccgctgtcaa gttcaagcgc
     1801 ttcgcaggcc ttgaggtcca ggtcggaata gatgctggtg atttcgccag ccgcagccgc
     1861 gcttgccgaa aggcccaaga caattagagc tgcgaatttt cccatggccc gtagtatgct
     1921 acgggaaatc gtgcaaagga aagccccatg cgtatcgacg ccattgccgc cggccggaac
     1981 ccgcccaagg aaatcaacgt catcgtcgaa gtgccggtgg gcggcgagcc gatcaaatac
     2041 gagatggaca aggaggcggg aacccttgtg gtcgaccgct ttctctatac ctccatgcgc
     2101 tatcccggga actacggctt cattccgcat accctgtccg gcgatggcga cccggtcgac
     2161 gtgctgatcg ccaaccagcg cggcatcatt cccggcgccg tcgtggcctg ccgcccggtg
     2221 ggcgtcctga agatgcagga cgaggcgggc ggcgatgaaa agatcattgc cgtgccggtg
     2281 ccccgcctca cccggcgcta tgaaaatgtt ttcaactatc tcgacctgcc ggaaatcacc
     2341 gtgcagcaga tcgagcattt cttcacccac tacaaggatc ttgaagcggg caaatgggtg
     2401 aaggtgctgg gctggggcaa cgccggggaa gccgagaaaa tgatcaccga cggcattgca
     2461 cgggccaagg cggcgaagaa atagttcgcc ttatttgcca ttccggcgaa cgcccccaaa
     2521 tttcggtcgc cgggatgacc gaagtggggt ggttggcatt ttaatccgta tacggatgat
     2581 cagctcgtgt cgggcatcgc ggcgcgatgg gcgagccact ggcattcgtc gagcagctcg
     2641 tcgacgaggt gaacggctcg cctgcgggaa cccggcgggc gcccggggcg gagcggcgtt
     2701 ttgaagctcg ggttttctga cttttcctcc cgcatccgcc agcgcaccag gcgcttggcc
     2761 tggcggggaa ggtcttggag ggcagcctcg agggcctcga gccggcggat gactcgcgcc
     2821 gcattgacca tcccatcagc ggatttggcc cgggcgggag cttttgccgg ccggatcggc
     2881 ggcccgatgg ttataaactc gccatcggta ttgaaaaagt ggatgcgggg gacagcccgc
     2941 ggagtccttt tccggcgcgg cagcatgatg cgtggctgcg ggtcgaaaag ctggaaggag
     3001 agggagcgct tatatccgcc tctccttgct ctttttgccc cggctggccc ggggcgggaa
     3061 gctgggggtt caaccacaag ccctcgcgcc gcaacgacga tgagccgccg cagggcggat
     3121 tcagcggggc ggagaacccg cagcacggcc cgataggcgg gccacggaat ccgcgaaacc
     3181 gtagcttcgc ccacaagtcc cagcatgaca aagagcgttt caacgatccc cgcgagtacc
     3241 tcgctgttgc gtttgatggc gtgctcccag tccatatttc cctatcctgt ctaaagaaca
     3301 aaacaggaac ataaatgaca tggtgggaat tcaatgtcaa gcattaaatt cctgtcagtg
     3361 gttacatcag ctgatgttat tttttgaatt gaggctggca atgatcaccc gcggatgctg
     3421 cgcgaggtaa ttgcaaggct tgccgctgac cgatttgcgc gcaaataagc gcaatcgtgc
     3481 catcacgata ttgaacacaa atgcaattct tggtagaatg cgcggcttat ctgcgtagtg
     3541 atcatccatg gtggtcccct tgatgtttgg ccaggcaatc atggggcagt gatgcaaatc
     3601 cagcagaaaa acagcgtaaa accagcgcaa atgcgtggaa aaatgggtat tcgggagcaa
     3661 gaaaacgcct cagccgacgc tattggcaca ggacagctca aaggctgccg gctggtcctc
     3721 gccttgctcg gccgcttttc ggccatgggt cc
//
LOCUS       DAORVN010000123         3746 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_19802_length_3746_cov_2.840423, whole genome shotgun sequence.
ACCESSION   DAORVN010000123 DAORVN010000000
VERSION     DAORVN010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3746
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19802_length_3746_cov_2.840423"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..653
                     /locus_tag="PKV23_03485"
     CDS             <1..653
                     /locus_tag="PKV23_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008124197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HQX84529.1"
                     /translation="LESMELCAKFCKLIREAVGTKADLLFGTHGQFTAAGAIRFAKHI
                     EPYDPLWFEEPTPPEKPEEMALVARGTSIPIATGERLTTKYEFGRVLECRAASILQMA
                     LGRVGGIWEAKKIAGMAETYYAQIAPHLYAGPVEAAANIHFATSLPNFLILESIETFG
                     GFYAKLLKKPIRWEQGYVIPPTEPGLGIELNEEVALAHPYTGDRLHLEMTNHPVNV"
     gene            650..1549
                     /locus_tag="PKV23_03490"
     CDS             650..1549
                     /locus_tag="PKV23_03490"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019860848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HQX84530.1"
                     /translation="MKYGYIGLGNLGGHLAASLLRNGFDVTVNDIDKSLAARHLKAGG
                     KWADTPKSLAEKVDCVITCLPSPAISEKVLAQVLEGLKPGSTWIEMSTLGRDEIQRLA
                     KLAGAKGMRTMEAPVTGGVHLAAQGKITVLAGGDTDLFELHKPALQAMGGQLFHMGPL
                     GSAAIIKVITNMLAFIHLVADGEALMLAKRGGLDLAQSWAAIKASSGTSFVHETEGQL
                     ILNGSYDIAFTMDLALKDLGFAMNFGREFGVPLDLAALTNETFIKGRQAYGGSAQSTQ
                     IVKLLEDALATDLRAPGFPARLE"
     gene            1667..1999
                     /locus_tag="PKV23_03495"
     CDS             1667..1999
                     /locus_tag="PKV23_03495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper domain-containing protein"
                     /protein_id="HQX84531.1"
                     /translation="MTRSRIFAAALATLLVAGPLAAESNAAGKRYCQDVAHDYAKRKT
                     GAQQVVPGLVGGAIAGGLIGSVIGGKKSVRTGVLVGGATGAVVGAGHANQKYDKYYWR
                     KYNECRQW"
     gene            complement(2088..3509)
                     /gene="glnA"
                     /locus_tag="PKV23_03500"
     CDS             complement(2088..3509)
                     /gene="glnA"
                     /locus_tag="PKV23_03500"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018698239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HQX84532.1"
                     /translation="MAKETKDVAAALKMIKDKEVKFVDLRFTDPRGKMQHLTMDVSQM
                     GEDAWADGLMFDGSSIAGWKAINESDMVLMPDPTSVHMDPFYAQTTMAVFCDILEPST
                     GEGYARDPRMVAKRAEAYLKQTKVGDTVFVGPEAEFFIFDDVKFSTNPYKTGFELDSV
                     EFPKNSDRDYETGNMGHRMRTKGGYFPMNPMDSAQDMRSEMLSVMGEMGLNIEKHHHE
                     VASAQHELGTKFQPLTMAADHMQIYKYVIHNVAHAYGKTATFMPKPVFGDNGSGMHCH
                     FSIWKEGKPMMAGNQYADLSELCLFFIGGVIKHAKALNAFTNPSTNSYKRLVPGYEAP
                     VLLAYSSRNRSASCRIPHTSNPKAKRVEVRFPDPTANPYLAFSAVVMAGLDGIANKMH
                     PGPAMDKNLYDLPPQELSQIPTVCGSLQEALDSLKADHEFLTKGGVYTKDQIEAYIDL
                     KQEEQDRYRMTPHPVEFDMYYSC"
     gene            complement(3540..>3746)
                     /locus_tag="PKV23_03505"
     CDS             complement(3540..>3746)
                     /locus_tag="PKV23_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018698238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HQX84533.1"
                     /translation="LYRGAEYVVDFLPKVKIEIVIADEFVDRAVEAIQTAARTGRIGD
                     GKIFISSIEQAIRIRTGESGADAL"
BASE COUNT          750 a         1143 c         1067 g          786 t
ORIGIN      
        1 cgctcgaatc catggaactc tgcgccaagt tctgcaaact catccgagag gccgtaggca
       61 ccaaggccga tcttctgttt ggcacccatg ggcagttcac cgccgctggc gccatccgct
      121 ttgccaagca catcgagccc tatgatcccc tctggttcga ggagccaacc ccgccggaga
      181 agccggagga gatggcgctc gtcgcgcgcg gcacgtccat tcccatcgcc actggcgaac
      241 gcctcaccac caaatatgaa ttcggccggg ttctggaatg ccgcgccgct tccattctgc
      301 aaatggccct gggccgcgtc ggcggcatct gggaagccaa gaaaatcgcc gggatggcag
      361 aaacctacta cgcccagata gccccgcatc tttatgccgg cccggtcgag gccgccgcca
      421 acattcattt cgccacctcg cttcccaact tcctcatcct tgaaagcatc gaaaccttcg
      481 gcggcttcta tgccaaactc ttgaagaagc ccatccgttg ggagcagggc tatgtcatcc
      541 cgcccaccga accgggcctc ggcatcgagc tgaatgagga ggtggcgctc gcccacccct
      601 ataccggcga taggctgcat cttgaaatga ccaatcatcc ggtgaatgta tgaagtacgg
      661 ctatatcggt ttgggcaatc tcggcggcca tcttgccgca agccttctgc gcaatggctt
      721 tgacgtcacc gtcaatgaca tcgacaaaag cctggccgcc cggcacctga aagccggcgg
      781 caagtgggcg gacacgccaa agtcgctcgc cgaaaaggtg gattgtgtca tcacctgtct
      841 gccgtctccc gccatatctg aaaaagtcct tgcccaggtc ctcgaaggcc tgaagcccgg
      901 atcgacgtgg attgaaatgt ccaccctggg ccgcgatgaa atccagcgcc tcgccaagct
      961 tgccggggcg aaaggcatgc gcaccatgga agctcccgtc accggcggcg tccacctcgc
     1021 cgcccaaggc aagatcaccg ttctcgccgg gggcgatacg gaccttttcg aattgcacaa
     1081 gcccgccctg caggccatgg gcggccagct ctttcacatg gggccgctgg ggtcagccgc
     1141 catcatcaag gtcatcacca acatgctggc cttcattcat ctcgtggccg atggcgaagc
     1201 cctgatgctg gccaaacgcg gtgggctcga tctcgcccaa agctgggccg ccatcaaggc
     1261 cagcagcggc acgagcttcg tccatgaaac cgagggccag ctcatcctca acggcagcta
     1321 cgatattgcc ttcaccatgg acctggccct gaaggacttg gggtttgcca tgaatttcgg
     1381 ccgcgaattc ggcgtgccgc ttgatctggc cgccctgact aatgaaacct tcatcaaggg
     1441 caggcaagcc tatggcggca gcgcccagtc cacccagatt gtcaagctcc tggaggatgc
     1501 gctggctacc gatttgcgcg ccccgggttt ccccgcgagg ctcgagtaac cataaataac
     1561 ccgtaaaaca gcccggaaat cccttacttt cgccacaccg caagagtgtc tatactcaat
     1621 ccaccgcagg agggggctcc tgagtccatg agaggaattc gaaattatga cccgaagcag
     1681 aatttttgcc gccgcccttg ccacgctttt ggtagctggc cccttggcgg ccgagtccaa
     1741 tgccgccggc aagcgctatt gccaggacgt ggcccatgat tatgcaaagc gcaaaaccgg
     1801 tgcccagcag gttgtgcccg ggctggttgg cggcgctata gccggcggcc taattggctc
     1861 ggtgattggc ggcaagaaat ccgtccgcac cggcgtcttg gtcggtggtg ccacaggcgc
     1921 agtcgtaggc gcaggccatg cgaatcagaa gtacgacaag tactactgga gaaagtacaa
     1981 cgaatgccgc cagtggtaag ctttgaagtc tgaaatcaaa aaaggccgga agttttcttc
     2041 cggcctttaa gtttagcggg gagacacccg cccttggctg cctaacctta gcagctgtaa
     2101 tacatgtcga actcgaccgg atgcggcgtc atgcggtaac ggtcctgctc ttcctgcttg
     2161 aggtcgatat aggcctcgat ctggtccttg gtgtacacgc cgcccttggt gaggaactca
     2221 tggtcggctt tcaggctgtc caacgcttcc tggagcgagc cgcacaccgt cgggatctgc
     2281 gacagttcct gcggcggcag gtcatagaga ttcttgtcca tggccgggcc cggatgcatc
     2341 ttgttggcaa tgccatcaag ccctgccatc accacggccg agaaagcgag ataggggttc
     2401 gccgtcggat cggggaaccg cacctcgacg cgcttggcct tcgggttgct cgtgtgcgga
     2461 atgcggcacg aagccgaacg gttgcgcgac gaataggcca gaagcaccgg cgcctcataa
     2521 cccggaacca gccgcttgta gctgttggtc gaagggttgg tgaaggcatt cagcgccttg
     2581 gcatgcttga tcaccccacc aatgaagaac aggcagagtt cggagagatc ggcatactgg
     2641 ttgcccgcca tcatcggctt gccttctttc cagatggaga agtggcagtg catgcccgaa
     2701 ccgttgtcgc cgaataccgg ctttggcatg aaagtcgccg tcttgccata agcgtgcgcc
     2761 acgttatgga tcacatactt gtaaatctgc atgtggtcgg cagccatggt cagaggctgg
     2821 aacttggtgc caagttcgtg ctgggccgat gccacctcat ggtggtgctt ctcgatgttc
     2881 agccccatct cgcccatgac ggaaagcatc tcggaacgca tgtcttgcgc cgagtccatc
     2941 gggttcatcg ggaaatagcc gcccttggtg cgcatccggt ggcccatgtt gccggtctcg
     3001 taatcgcggt ccgagttttt cgggaactcc actgagtcca gttcaaagcc ggtcttgtag
     3061 gggttggtcg agaatttcac gtcgtcgaag atgaagaatt cggcttccgg tcccacgaag
     3121 actgtgtcgc cgacttttgt ctgcttgagg taagcctcgg cccgcttggc gaccatccgc
     3181 ggatcacgcg cgtagccttc gccggtcgat ggttcgagga tgtcgcagaa caccgccatg
     3241 gtcgtctggg catagaaagg gtccatgtga accgaagtcg gatctggcat cagcaccatg
     3301 tcggactcgt tgatggcttt ccatcccgcg atcgacgagc catcgaacat cagcccgtcg
     3361 gcccaggcat cttcgcccat ctgcgacaca tccatggtga gatgctgcat cttgccgcgc
     3421 ggatcggtga agcgcaggtc gacgaatttc acttccttgt ccttgatcat cttcaaggct
     3481 gctgcaacgt cttttgtctc tttggccatt atgttttcct ctttgattta tggcgtgaat
     3541 tacagtgcat ccgccccgga ctcgccggtc cggatgcgga tcgcttgttc gatgcttgaa
     3601 atgaaaatct tgccgtcgcc gatgcgcccg gtgcgcgccg cagtctggat ggcctctacg
     3661 gcgcggtcga cgaattcgtc ggctatgacg atttcgattt taaccttggg cagaaaatcc
     3721 accacatatt cggccccgcg gtaaag
//
LOCUS       DAORVN010000124         3740 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_19854_length_3740_cov_3.283039, whole genome shotgun sequence.
ACCESSION   DAORVN010000124 DAORVN010000000
VERSION     DAORVN010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3740
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19854_length_3740_cov_3.283039"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1165)
                     /gene="trpB"
                     /locus_tag="PKV23_03510"
     CDS             complement(<1..1165)
                     /gene="trpB"
                     /locus_tag="PKV23_03510"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011923362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HQX84534.1"
                     /translation="MTTAQVNSYRTGPDEKGLFGIYGGRFVAETLMPLILELEQAYKD
                     AKADPSFQAELDSFLEHYVGRPSPLYYAERLTEHLGGAKIYFKRDELNHTGAHKINNV
                     MGQILLARRMGKKRIIAETGAGQHGVATATACARFGLECIVYMGSVDIERQKPNVFRM
                     KLLGAEVVPALSGSRTLKDALNEALRDWVANVEDTFYCIGTVAGPHPYPEMVRDFQSV
                     IGIETKRQMLAREGRLPDTLVAAIGGGSNAMGLFHPFLDDKEVEIIGVEAAGLGLRTR
                     KHAASVTGGRPGILHGNRTYLLMDDDGQITEGHSISAGLDYPGIGPEHSWLHDMKRVQ
                     YVAATDKEALAAFQLCCRLEGIIPALEPSHALAHVMKIAPTKPKDHLMVMNMCG"
     gene            complement(1189..1836)
                     /locus_tag="PKV23_03515"
     CDS             complement(1189..1836)
                     /locus_tag="PKV23_03515"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012332365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HQX84535.1"
                     /translation="MVDVKICGLSTMESVDAAIEAGAGLVGFVFYPRSPRHVSFPLAK
                     KLAAHARGRAKIVALIVDANDVALQEIDNIVAPDLYQAHGAETPERVQEIAALTGKPV
                     IKAIKIGASADAARADAYAGKASLILYDAKAPETLVNALPGGNGIAFDWSLLGSERQP
                     EGFILSGGLNPENVAKAIRATGAPIVDVSSGVEKAPGVKDLSLIRKFIEAAKAAR"
     gene            complement(1829..2533)
                     /gene="pyrF"
                     /locus_tag="PKV23_03520"
     CDS             complement(1829..2533)
                     /gene="pyrF"
                     /locus_tag="PKV23_03520"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011995059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HQX84536.1"
                     /translation="MSEANPICVALDTPDIARAQTLARQLRPHVGYLKIGMEFFYAHG
                     SRGYEAVAAAGLPIFLDLKLHDIPNTVAEGMRSLMRLSPSPALINLHASGGMAMMQAA
                     AEAVGGRAKLIAVTLLTSLSDEDIWAAGFDAERDTGAHAAGLAKLAKSAGLDGVVCSA
                     HDLAGIRAATGPEFLTVVPGIRPTENAVHDQKRVATPKSAIAAGADILVIGRAITGAA
                     DPAAAAQKILEDLRHG"
     gene            complement(2558..3478)
                     /locus_tag="PKV23_03525"
     CDS             complement(2558..3478)
                     /locus_tag="PKV23_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013893004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HQX84537.1"
                     /translation="MKVISAEALAKVATYGAIVEALREGFRADIATPVRHHHETSAVS
                     TLLLMPAWSTEWTGLKTVVVKSDNAAKNLPTVQASYLLIRNDTGETVAIMDGTELTRR
                     RTAAASALAADYLARPDASTLVLVGAGALGAHFVRAHAAVRPIKRVFVFNRSPEKAAG
                     LARDLKADGLEASAVTDLEAAVRQADIVTCVTTSTKPIVKGAWLKPGTHVDLAGAFKP
                     SMRETDGEVVARARVFVDTREGALSEAGDLLQARDEGKFDFARVQGDLFDLCRGSVKG
                     RKTPGEITLFKSCGTALEDLATAIMVYLRS"
     gene            complement(3475..>3740)
                     /locus_tag="PKV23_03530"
     CDS             complement(3475..>3740)
                     /locus_tag="PKV23_03530"
                     /inference="COORDINATES: protein motif:HMM:NF018058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HQX84538.1"
                     /translation="AIANRQWITVSFDPFSRDAPFAAMDMPLWLLFFCGGFFGLIAGW
                     IACWVAQGKWRRAAKDTRRELAHAQTEASELRREVPPVDHSFL"
BASE COUNT          704 a         1120 c         1197 g          719 t
ORIGIN      
        1 ggccgcacat gttcatcacc atcaagtggt ccttgggttt tgtcggcgcg atcttcatca
       61 catgggcgag cgcgtgggaa ggctcgagag ctgggatgat tccttccagc ctgcagcaga
      121 gctggaaggc ggccaatgct tccttgtcgg tggcggccac atattgcacg cgcttcatgt
      181 cgtgcagcca ggaatgctcg gggccgatgc cgggataatc aaggcctgcc gagatggaat
      241 ggccctcggt gatctggccg tcatcatcca tcagcagata ggtgcgattg ccgtggagaa
      301 tgccgggcct gccgccggtg acggaggccg catgtttcct ggtacgcagg cccaggcccg
      361 ccgcttccac gccgatgatt tccacttcct tgtcgtcgag gaagggatgg aacaggccca
      421 tggcattgga gccgccgcca attgcagcga caagcgtgtc cggcaggcgg ccctcgcggg
      481 ccagcatctg gcgcttggtt tcaatgccga tcaccgactg gaaatcacgc accatttccg
      541 gatagggatg ggggcccgcc acggtgccga tgcaatagaa ggtgtcttca acattggcga
      601 cccaatcgcg cagcgcttcg ttcagcgcat ccttcaaggt gcgcgaaccg gacagggcgg
      661 gaacgacttc ggcccccagc agtttcatgc ggaacacgtt tggtttctgg cgttcgatgt
      721 cgaccgatcc catgtagaca atgcattcca ggccgaaacg ggcgcaggca gtggcggtcg
      781 cgacgccgtg ctggccggcg ccggtttcgg cgatgatgcg cttcttgccc atgcgccggg
      841 caagaaggat ctgacccatg acattgttga tcttgtgagc cccggtgtgg ttgagctcat
      901 cgcgcttgaa atagattttt gcgccgccca ggtgttcagt caggcgctcg gcataataga
      961 gcggactggg ccttcccaca taatgctcaa ggaatgaatc aagctcggcc tggaagctgg
     1021 gatcggcctt ggcgtccttg taggcctgct caagctcaag gatgagcggc atcaatgttt
     1081 cggccacgaa acgcccgcca tagatgccga acaggccttt ttcgtcgggc ccggtgcggt
     1141 aggaattaac ctgtgctgtg gtcataaaaa ctcgctttca aagccgtttt agcgggccgc
     1201 cttggccgcc tcaatgaatt tccgaatgag gcttaaatcc ttcacgccag gagccttttc
     1261 aacacctgag gagacatcga caatgggcgc cccggtggcg cggatggcct ttgcgacgtt
     1321 ttccggattg agcccaccgg agagaataaa gccctcaggc tggcgctcgc tgcccagcag
     1381 gctccagtca aaggctatgc cgttgccgcc gggcagcgca ttaaccaacg tctcgggagc
     1441 cttcgcgtcg taaagaatga ggctggcctt gccggcgtag gcatctgccc ttgcagcatc
     1501 ggctgaggcg ccaatcttga tggccttgat caccggcttg ccggtcagcg cggcgatctc
     1561 ctgcacgcgc tccggcgttt ccgcgccatg ggcctgatac aggtcggggg ccacgatatt
     1621 gtcgatttcc tggagcgcca catcattggc atcgacgatg agggcgacga tttttgcccg
     1681 gccgcgcgca tgggcagcaa gcttctttgc cagcggaaaa gagacatggc gggggctgcg
     1741 gggataaaac acgaagccga caaggccggc gcctgcctcg attgccgcat caaccgattc
     1801 catggtggag agcccgcaaa tcttcacatc aaccatggcg caggtcttcc agaatctttt
     1861 gcgcagctgc ggccggatcg gcagcgccgg tgatggcccg gccgatcaca agaatatcgg
     1921 cgccggcggc gatggccgat ttgggcgtgg cgacacgctt ctggtcatga acggcatttt
     1981 cggtggggcg gatgccgggc acgacggtca aaaattccgg gccggtcgcg gcacggatgc
     2041 ctgcaagatc atgggcgctg cacacaacac cgtccagccc ggctgatttg gcaagctttg
     2101 caagtcccgc agcatgggcg cccgtatccc gttccgcatc gaagccggcg gcccagatgt
     2161 cctcatcgga gaggctggtc aaaagggtca cggcaatcaa cttggcgcgg ccgcccaccg
     2221 cttccgccgc cgcctgcatc atggccatgc cgccgctggc atggagattt atcagggccg
     2281 gacttgggga aagccgcatg agggagcgca tgccttcggc caccgtattg ggaatgtcgt
     2341 ggagcttgag atcgaggaat atgggcaggc ccgcggcggc cacggcctca tagccccggc
     2401 tgccgtgggc gtagaaaaat tccatgccga ttttcaggta gccgacatgg ggcctcagtt
     2461 gcctggcaag ggtctgggcc cgggcaatat ccggcgtatc aagggccacg cagatggggt
     2521 tggcttcgct catggggcac tcgatagccg caaacagtca ggagcgcaaa tagaccatga
     2581 tggcggttgc gaggtcctca agggctgtgc cgcaggactt gaagagggtg atttcgccgg
     2641 gcgttttccg ccccttcaca ctgccccggc agaggtcaaa gagatcaccc tggacccttg
     2701 cgaaatcaaa cttgccctcg tcgcgggcct gcagcaggtc gcccgcttcg gacagggcgc
     2761 cctctctggt atccacaaaa acccgggcgc gggccaccac ctcgccatcg gtttcgcgca
     2821 tgctgggctt gaaggcgccg gcgagatcca catgggtgcc gggcttcagc catgcgccct
     2881 tgacaatcgg ctttgtcgat gtcgtcacgc aggtgacaat atctgcctgg cgcacggccg
     2941 cttccagatc ggtgacggct gatgcctcaa gcccgtcagc tttcaagtca cgtgccaggc
     3001 ctgccgcctt ttccggtgag cggttgaaaa caaagacccg cttgatgggc cgcactgccg
     3061 cgtgggcgcg cacgaaatgc gcgcccaggg caccggctcc caccagcaca agcgttgaag
     3121 cgtcgggcct tgccagatag tcggccgcca gggccgaggc ggcggcggtc cgccggcggg
     3181 tgagttcggt gccgtccatg atggccacgg tttcgcccgt gtcattcctg ataagaagat
     3241 agctcgcctg aacggtgggc aggttttttg cagcattgtc tgatttgacg acaacggttt
     3301 tcaatccggt ccactctgtg gaccaggccg gcatcagcag cagggttgaa accgcagacg
     3361 tctcgtgatg gtggcggacg ggggttgcga tgtcggcccg gaagccttcg cgcaaggctt
     3421 cgacgattgc gccataggtt gcgactttcg ccagcgcctc agccgatatg actttcatag
     3481 gaagctgtgg tccacgggcg ggacttcacg gcgcagttcg ctcgcctccg tttgggcgtg
     3541 cgccagttcc cggcgcgtgt ccttggcggc gcggcgccat ttgccctggg ccacccagca
     3601 ggcaatccag ccggcaatca ggccaaaaaa acccccgcag aagaacagca gccagagggg
     3661 catgtccatg gcggcaaagg gcgcatcgcg cgagaaggga tcaaatgaaa cagtgatcca
     3721 ctggcggttg gcaatggcga
//
LOCUS       DAORVN010000125         3735 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_19905_length_3735_cov_2.481250, whole genome shotgun sequence.
ACCESSION   DAORVN010000125 DAORVN010000000
VERSION     DAORVN010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3735
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19905_length_3735_cov_2.481250"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(98..1747)
                     /locus_tag="PKV23_03535"
     CDS             complement(98..1747)
                     /locus_tag="PKV23_03535"
                     /EC_number="1.5.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009452214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004174 -
                     electron-transferring-flavoprotein dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HQX84539.1"
                     /translation="MEQVQRETMEYDVVIVGAGPAGLAAAIRLKQLSSDISVCILEKG
                     SEVGAHILSGAVVDPIALNELIPDWKEKGAPLNTPVTEDRFYVLGPKGALRMPNFLMP
                     PLMNNHGNYAVSLGNICRWLATQAEALGVEIYPGFACSEVLYRDDGSVRGVVAGVMGI
                     AKDGSHKSDYQPGMELIGKYVFIAEGVRGSLAKEIIAKYKLSEGREPQKFGLGMKELW
                     EVKPENHKQGQVTHTMGWPLGMKTGGGSFMYHLEDNLVSIGFVVHLNYQNPYLYPYME
                     FQRFKHHPLISQVLEGGRRVAYGARAITEGGFQSVPKLYFPGGVLIGCSAGFVNVPRI
                     KGSQNAMKTGMMAAEAAFAALKEGRAGDELAAYETAYEKSWVYRDLKRVRNVKPMWSK
                     LGLVGGVVLGAVDMWLNTLFGFGFGTWKHGKPDSATLKPAADSTIINYPKPDGVLSFD
                     RLTNLAFSATNHEEDEPPHLKLKDASIPIAINLPMWAEPAQRYCPAGVYEVIMEDGKP
                     PRFQINHQNCVHCKTCDIKDPSQNINWVVPQGGEGPNYPNM"
     gene            1854..2588
                     /locus_tag="PKV23_03540"
     CDS             1854..2588
                     /locus_tag="PKV23_03540"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HQX84540.1"
                     /translation="MSPGDAQAALAWLIDMGADEIVAEAPVNRFAAAAPPVAAVPPAE
                     AAPAVFVPLSADACNSLDELQAALLALDDFPLKKTASNLCFAGGDATAQVMIIGDVPG
                     RDEDIEGKVFAGQNLLLLERMLGAIGLSVAATSSFNFIPWRPPGNRTPTDAELQLCQP
                     YILRAIALCRPKFILCLGGHPAQRLSGKSDGLLSLRGKWFELAIGAKGIPVLPSFHPS
                     YLLKQPGQKRLAWRDLLSFKEALDAH"
     gene            2578..3204
                     /locus_tag="PKV23_03545"
     CDS             2578..3204
                     /locus_tag="PKV23_03545"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HQX84541.1"
                     /translation="MPISSTALVIFLTAALSLNLTPGNDMMYVLGQSLKGGPRTGISA
                     SLGIAGGSLIHLGLVALGVAVVLAQNPLLFDLIRYAGAAYLVWIAYGTLTSPLTALNR
                     DETERSTFAAFRDGVLVNLLNPKIIVFMFAFLPPFIRPENGSPLLQLFILGMIFNVGG
                     TIINCVVALFAGKIGAALSTSLKFRRWFSRISAGLFLLLAARLVFERK"
     gene            3205..>3735
                     /gene="moaB"
                     /locus_tag="PKV23_03550"
     CDS             3205..>3735
                     /gene="moaB"
                     /locus_tag="PKV23_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007594989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HQX84542.1"
                     /translation="MTEPRDFLPLNIAILTVSDTRTLSDDKSGQTLQERIAAAGHSVA
                     DRQITKDEVAKIRRIVKAWVKRKDVDVIISTGGTGLTGRDVTIEAMRPLFEKEIDGFS
                     TLFHMVSFPKIGTSTVQSRATAGVAKGTYIFCLPGSPGACRDAWDEILKYQLDYRHPP
                     CNFVEIMPRLEEHQKRR"
BASE COUNT          722 a         1077 c         1114 g          822 t
ORIGIN      
        1 ggaaaggttg ctttttagac actttgtaaa atttcaaaag aaacgcatcc aaggctaatc
       61 cctgcttaac ggatgcctgc aaatcgcggc cggcctgtta catgtttgga tagtttggcc
      121 cctcgccgcc ctgcggcacg acccagttaa tgttttgtga aggatctttt atgtcgcagg
      181 ttttgcaatg gacgcaattc tggtggttga tctggaaacg gggcggtttg ccgtcctcca
      241 tgatcacttc atagacccct gccgggcaat agcgctgggc cggctcggcc cacatgggca
      301 gattgattgc aatcggaatg cttgcatcct tcaatttgag gtgaggcggc tcgtcttcct
      361 catgattggt ggcggaaaaa gcaaggttcg tgaggcggtc gaaggacagc acgccatcgg
      421 gcttgggata gttaataatc gttgaatcgg cggcgggctt cagtgtggcg ctgtcgggct
      481 tgccatgttt ccaagtgccg aagccaaaac caaacagggt gttgagccac atatccacgg
      541 cgccgagaac gacgccgcca acgagcccga gcttcgacca catgggcttc acgttcctca
      601 cccgcttcag gtcccggtag acccaggact tctcgtaagc tgtttcataa gcggccaatt
      661 catcgccggc gcggccttct ttcaaggctg cgaaggcggc ttcggcggcc atcatgccgg
      721 tcttcatggc gttctgcgag cccttgatcc ggggcacgtt cacaaagccg gcggagcagc
      781 cgatgaggac gccgccgggg aaatagagct tcggcacgga ctggaagcca ccctcggtga
      841 tggcccttgc gccataggcc acgcgcctgc cgccttcgag gacttgcgag atcagcgggt
      901 ggtgcttgaa gcgctggaat tccatgtagg ggtagagata ggggttctgg taattgagat
      961 ggacgacgaa gccaatggaa acgagattgt cttccaggtg gtacatgaag cttccgccgc
     1021 ctgttttcat ccccaggggc cagcccatgg tgtgggtcac ttgaccttgc ttgtggtttt
     1081 cgggcttcac ttcccacagc tctttcatgc caaggccgaa tttctgcggc tcgcggcctt
     1141 ccgacaattt gtatttggcg atgatttcct tggccagcga accgcgcaca ccttcggcga
     1201 tgaaaacata tttgccgatg agttccatgc cgggctggta gtcggatttg tggctgccgt
     1261 ccttggcgat gcccatgact ccggccacca cgccgcgcac cgagccatcg tcgcggtaga
     1321 gaacttccga acaggcaaag ccgggataaa tctcaacacc gagggcttcc gcctgggttg
     1381 ccagccagcg gcagatattg ccgagcgaca cggcgtagtt gccatgattg ttcatgagcg
     1441 gcggcatcag aaaattcggc atgcggagcg cgcccttggg acccagcaca tagaaacggt
     1501 cctcggtcac cggcgtattc agcggtgcgc ccttttcttt ccagtcaggg ataagttcat
     1561 tgagggcgat gggatcgacc acggcgccgg agaggatatg ggcgccgact tccgaacctt
     1621 tttccagtat gcaaaccgag atatcggagg acaactgttt caggcggatg gcggcggcga
     1681 ggccggcagg gccggcgccc acgatgacaa catcatattc catcgtttcg cgctgaacct
     1741 gctccacaga caattctcct caaagggcat gcgtttccgg acagcgcctg ttatagtggc
     1801 ccgacccggc agacaagata accaattcgg acatgcaaac aaagcaaaac gacatgagcc
     1861 ccggtgatgc ccaggcggca ttggcctggc tcatcgatat gggggccgac gagattgtgg
     1921 ccgaggcccc ggtcaatcgc tttgcggcgg cggccccgcc tgtggctgcc gttccgcccg
     1981 ccgaggcagc gcccgccgtt tttgtccctt tatcggcaga cgcctgcaat tcgctggacg
     2041 agcttcaggc ggcattgctt gccctggacg atttcccgct gaaaaagacc gcgagcaatc
     2101 tgtgctttgc cggtggcgat gccacggcgc aagtgatgat catcggcgat gtcccgggac
     2161 gcgatgagga catcgagggc aaggtgtttg ccgggcaaaa tcttcttctg ctggaaagga
     2221 tgctgggggc gatcgggttg tcggttgcgg ccaccagttc attcaatttc attccgtggc
     2281 ggccgccggg aaaccgcacg ccaaccgatg ccgaactgca actgtgccag ccctatatcc
     2341 tgcgggccat tgcgctttgc cggccgaaat tcatcctctg cctgggcggc catccggccc
     2401 agcgcctgtc gggcaagagc gacggactgc tttcgctgcg cggcaaatgg ttcgagctag
     2461 ccatcggggc aaagggaatt ccggtgcttc ccagttttca tcccagttac ctgttgaagc
     2521 agccggggca gaagcgcctg gcctggcgcg atcttctttc gttcaaggaa gcccttgatg
     2581 cccattagct ccacggccct ggtgattttc ctgacggcgg cgctgagcct caatctgacg
     2641 ccgggcaatg acatgatgta tgttctgggg caatccttga agggcgggcc acggaccggc
     2701 atttcagcaa gccttggcat tgcgggtggc tcgctgatcc atctcggcct cgtggcgctg
     2761 ggtgttgccg ttgtcctggc gcagaacccg ctgctcttcg atcttatccg ctatgcgggt
     2821 gcggcctatc ttgtctggat tgcctacggc acgctcacct cgccgcttac tgcccttaac
     2881 cgggatgaaa cggaacgcag cacatttgcg gctttccgcg atggcgttct ggtcaacctg
     2941 ctcaacccca agatcattgt tttcatgttt gccttcctgc cgccgttcat ccggccggaa
     3001 aacggctcgc cgcttctgca gcttttcatc cttggaatga ttttcaatgt gggtggcacg
     3061 atcatcaatt gcgtggtggc gctgtttgcg ggaaaaattg gcgcggcgct ttccaccagt
     3121 ctgaaattcc gccgctggtt ttcacgcatc tcggccggcc tgtttctgct gctggcggcg
     3181 cgccttgtgt ttgagagaaa atagatgact gagccccgcg atttccttcc cctcaacatt
     3241 gcaatcctca cggtgtcgga cacgcgcacg ctttccgatg ataaatccgg ccagaccctt
     3301 caggagcgaa ttgcggcggc aggccacagc gttgccgacc ggcagatcac caaagatgag
     3361 gtcgcaaaga ttcgccgcat cgtgaaagcc tgggtgaagc gcaaggatgt tgacgtcatc
     3421 atttcaacgg gcggcacggg acttacgggg cgcgatgtga ccatcgaggc gatgcgcccg
     3481 ctgttcgaaa aggaaatcga cggcttcagc accctgttcc acatggtctc gtttccaaag
     3541 atcggaacct caacggtgca atcgcgcgcc acggcgggcg ttgcgaaggg cacatatatt
     3601 ttctgcctgc cgggctcacc gggtgcgtgc agggatgcgt gggatgaaat cctgaaatac
     3661 cagttggatt accggcatcc gccctgcaac ttcgtggaaa tcatgccgcg cctggaggag
     3721 catcagaagc gccgg
//
LOCUS       DAORVN010000126         3734 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_19914_length_3734_cov_3.370753, whole genome shotgun sequence.
ACCESSION   DAORVN010000126 DAORVN010000000
VERSION     DAORVN010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3734)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3734)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3734
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19914_length_3734_cov_3.370753"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..990
                     /locus_tag="PKV23_03555"
     CDS             <1..990
                     /locus_tag="PKV23_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018447985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQX84543.1"
                     /translation="FAPIIANDRPMLVSYKGEVLFPTFVNYPESKFGGFLARTDYRDP
                     VNQDEINANGWMIWPPIRYSYNTVNNELPRPAPSTPASNLTREEACAKYPLGAADPNC
                     NFGNLNWLGTDDQGRDVVARLIYGFRISVAFGLILTFFSSIIGVAAGAVQGYFGGWTD
                     LLFQRFIDIWSSMPTLYLLIILAAFITPTFWLLLFILVLFSWTGLVVVVRAEFLRARN
                     LEYVRAARALGLSNTKIMFKHLLPNALISTVTFMPFILSGSVTTLTALDFLGFGLPPG
                     SPSLGELLAQGKANLQAPWLGFTGFFVVSIMLTLLVFIGEGVRDALDPRKTFA"
     gene            987..2615
                     /locus_tag="PKV23_03560"
     CDS             987..2615
                     /locus_tag="PKV23_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012709949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQX84544.1"
                     /translation="MSEVLLEVKDLSVAFRQGGKATLAVDHVSFSLKKGETLALVGES
                     GSGKSVSALSILKLLPYPAAEHPSGSVRFKGQELLDADESDLRKVRGNDITMIFQEPM
                     TSLNPLHSVEKQIGEILELHQGLGGAAARERIISLLSKVGIRDPETRLQDYPHQLSGG
                     QRQRVMIAMALANNPDLLIADEPTTALDVTVQAQILKLLKELQAENGMALLLITHDLG
                     IVRHMADTICVMQQGKVVEAGDAKAVFAKPQHEYTKMLLLAEPKGKPPKSDTSAPIVV
                     ETKNLKVWFPIKRGFFRSTVGHIKAVDGLDTLVRAGQTLGVVGESGSGKTTLGLAILR
                     LISSEGEISFQGQRIDKFSFNEMRPLRKDMQIVFQDPFGSLSPRLSILQIVEEGLLVQ
                     GGRGNAEERRAHVAQALTEVGLDPATMDRYPHEFSGGQRQRIAIARALALNPKFIMLD
                     EPTSALDMSVQAQIVDLLRALQTKHNLCYLFISHDLKVVRALANEVVVMRNGVAVERG
                     PASQIFENPQTDYTRALIAASLNLRVVPGNSVKQ"
     gene            2615..3562
                     /locus_tag="PKV23_03565"
     CDS             2615..3562
                     /locus_tag="PKV23_03565"
                     /inference="COORDINATES: protein motif:HMM:NF014841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase A"
                     /protein_id="HQX84545.1"
                     /translation="MAFLFVTPTWPSDLWKAAMNKAAPKLEVRIWPDGLGNAQDIEYT
                     AAWLPPANVLKGLPNLKVIFSLGAGVDAILKDPTLPAGIPIVRVNDDDLTKRMTEYIV
                     LHVLMHHRQQRRIDANQKQKIWDSFPTHAASALSVGIMGLGVMGQDSALRLRELGFRV
                     AGWSRSRKSIPGVDCFAGPAEFDAFLARSDILISLLPATAETDGIINRETILKLSRKG
                     PFGAPIIINAGRGRQQVASDILTCLDAGELHAATLDVFAVEPLPANDPLWSHPRVTVT
                     PHIAADSEPEVICAYVAAQIERHQKGLPLINLVDVKRGY"
     gene            complement(3559..>3734)
                     /locus_tag="PKV23_03570"
     CDS             complement(3559..>3734)
                     /locus_tag="PKV23_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012252579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HQX84546.1"
                     /translation="LRRGDLVFWKGHVGIMADQKTLLHANGHHMMTVKEPLSEAVARI
                     AGTYGQVTSIRRF"
BASE COUNT          713 a         1083 c         1149 g          789 t
ORIGIN      
        1 tttgcgccga tcattgccaa tgaccggccc atgctggtgt cctacaaggg tgaggtgctg
       61 ttcccgacct ttgtcaatta tcccgaatcc aagttcggcg ggttcctggc gcgtacagac
      121 tatcgcgatc cggtgaacca ggatgaaatc aatgccaatg gctggatgat ctggccaccc
      181 atccgctatt cctacaacac ggtgaacaac gaactgccgc ggcccgcgcc ttccacgcct
      241 gcctcaaatc tcacgcggga ggaggcctgc gccaagtatc cgctcggtgc tgcggatccc
      301 aactgcaatt tcggcaattt gaactggctg ggaaccgacg accaggggcg ggacgtggtg
      361 gcgcgtctga tttacggctt ccggatttcg gtggccttcg ggctgatcct cacattcttc
      421 tcgtcaatca tcggcgtggc ggcgggggcg gtgcagggct atttcggcgg ctggacggac
      481 ctgctgttcc agcgcttcat cgacatctgg tcatcgatgc caacgctcta tctgctgatc
      541 attctggcgg cattcattac cccgacattc tggctgctgc tattcatcct ggttctcttc
      601 tcgtggacgg gattggtcgt cgtggtgcga gccgaattcc tgcgggcccg caatctggaa
      661 tatgtgcgcg cggcccgcgc cctgggtttg agcaatacaa aaatcatgtt caaacatctc
      721 ctgcccaacg cgctgatttc aacagtcacc ttcatgcctt tcattctctc aggttcggtg
      781 acgacgctaa cggcccttga ttttcttggc tttgggttgc cgccgggctc gccttcgctg
      841 ggcgaattgc tggcccaggg caaggccaac ctgcaggcgc catggctggg gttcaccggc
      901 ttcttcgtgg tttccatcat gctcacgctt ctggtgttca tcggcgaggg cgtcagggat
      961 gcgcttgatc cgcgaaagac tttcgcatga gcgaggtttt gctcgaggtc aaggacctgt
     1021 ccgtggcctt ccgccagggc ggcaaggcca cgctggcggt tgaccatgtg tccttctccc
     1081 tgaaaaaagg cgagacgctg gcgcttgtgg gtgaatctgg ctctggcaaa tcggtttcgg
     1141 cactttccat tctcaagctc ctgccctatc cggcggcaga gcacccttcc ggttcggtca
     1201 ggttcaaggg ccaggaattg ctcgatgcgg atgagagcga cctccgcaag gtgcgcggca
     1261 acgacatcac gatgattttc caggaaccaa tgacctcgct caatccgctg cattcggtgg
     1321 aaaagcagat cggcgagatc ctggaattgc accagggcct gggcggcgct gcggcgcggg
     1381 aacggatcat cagcctgctc agcaaggtgg gcatccgcga tcccgagacg cgactgcagg
     1441 attatccgca ccagctttcc ggcgggcagc ggcagcgcgt tatgatcgcc atggcgctgg
     1501 ccaacaatcc cgacctgctc attgccgatg aacctaccac ggcgcttgac gtgacggtgc
     1561 aggcgcaaat tctcaagctg ctgaaggaac tgcaggccga aaacggcatg gcgctactgc
     1621 tcatcaccca tgaccttgga atcgtgcgcc acatggcgga cacgatttgc gtgatgcagc
     1681 aaggcaaggt ggttgaagca ggcgatgcca aggcggtgtt tgcgaagcct cagcatgaat
     1741 acacaaaaat gctgcttctg gcggagccca agggcaagcc gccgaagtcc gatacgagcg
     1801 ctcccatagt tgttgaaacg aagaacctga aagtctggtt tccgatcaag cgcggatttt
     1861 tccgcagcac ggtcggccat atcaaggcgg ttgacgggct tgatacgctg gtgcgggccg
     1921 ggcagaccct gggcgtggtg ggtgaatcag ggtcggggaa aaccacgctc gggcttgcga
     1981 tcctgcggct gatttcctct gagggtgaaa tttcattcca gggccagcgg atcgacaagt
     2041 tcagcttcaa tgagatgcgg cccctgcgca aggacatgca gattgtattc caggatccat
     2101 tcggctccct gtcgccgcgg ctttccattc tccagatcgt cgaagaggga ttgctggtgc
     2161 agggcgggcg cgggaatgcg gaggagcgcc gcgcgcatgt tgcgcaggcg cttacggaag
     2221 tgggcctcga ccctgcgacc atggaccgtt acccgcatga attttccggc gggcaacggc
     2281 agcgcatcgc cattgcaagg gccctggcac tcaatcccaa attcatcatg ctggatgaac
     2341 ccacctcggc gctggacatg tcggtgcagg cgcagatcgt cgatctgctg cgggcgctgc
     2401 agaccaagca taacctctgc tatctcttca tcagccatga cctcaaagtg gtgcgggccc
     2461 tggccaatga ggtggtcgtc atgcgcaatg gggttgcggt tgagcggggg ccggcgagcc
     2521 agatcttcga gaacccgcag acggactata ccagggcgct gattgcggcg tcgctcaacc
     2581 tccgggtcgt tccgggaaac agtgtgaaac aataatggca tttctctttg tgacgcccac
     2641 atggcccagt gacctctgga aggccgcaat gaacaaggca gcgccaaagc tggaggttcg
     2701 gatctggcct gatggtttgg ggaatgcgca ggatattgag tatacggccg cctggctgcc
     2761 gccggccaat gttctgaaag gcttgcccaa ccttaaggta attttttcct tgggggccgg
     2821 ggtcgatgcc atcctgaagg accccaccct tcccgccggc attcccatcg tgcgggtgaa
     2881 cgacgatgac ctcaccaagc ggatgacgga atacatcgtg ctgcatgtgc ttatgcacca
     2941 ccggcagcag cggcgcatcg atgccaacca gaagcagaag atttgggaca gtttcccaac
     3001 ccatgccgct tcggcgcttt cggtgggcat catggggctt ggcgtcatgg ggcaggattc
     3061 agcgctgcgc ctgcgcgagc tcggctttcg cgtggcgggc tggagccgca gccggaaaag
     3121 cattccggga gtagattgtt ttgccgggcc tgccgaattc gacgcattcc tggcgcgcag
     3181 cgatatactg atttccctgc ttccggcgac ggcggagacg gacggcatca tcaatcggga
     3241 gactattcta aagctgtcgc gcaaggggcc gtttggggcg ccaatcatca tcaatgcggg
     3301 ccgcggacgc cagcaggtgg ccagcgacat tctcacatgc ctcgatgcgg gcgagcttca
     3361 tgcggcgacg ctcgacgtgt ttgcggtgga gcccctgccc gccaatgatc ccctctggag
     3421 ccatccgcgg gtgactgtta cgccccatat tgcggcggat tcggagccgg aggtgatctg
     3481 tgcctatgtg gcagcccaga tcgagcggca tcaaaaggga ttgcctctta tcaatctggt
     3541 ggatgtcaaa cgcggatact agaatcgcct gatgctggtg acctgcccat aggtgccagc
     3601 gatacgggcg accgcttccg agaggggctc cttgacggtc atcatgtggt ggccattggc
     3661 gtggagcaaa gtcttctggt cggccatgat tcccacatgg cctttccaga acaccagatc
     3721 gccacggcgc aggc
//
LOCUS       DAORVN010000127         3732 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_19937_length_3732_cov_3.013326, whole genome shotgun sequence.
ACCESSION   DAORVN010000127 DAORVN010000000
VERSION     DAORVN010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3732)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3732)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3732
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19937_length_3732_cov_3.013326"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2526)
                     /gene="polA"
                     /locus_tag="PKV23_03575"
     CDS             complement(<1..2526)
                     /gene="polA"
                     /locus_tag="PKV23_03575"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011995019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HQX84547.1"
                     /translation="MSHSTSLKPGDHLYLVDGSGYIFRAYHALPPLTRKSDGLPVGAV
                     QGFCNMLWKLLKETRANEKPTHLAVIFDESSKTFRNDIYPEYKAHRPEVPADLRPQFG
                     LIRQATTAFNVACVEQANYEADDLIATYARQAVEAGATCRIVSSDKDLMQLVRKGVTL
                     LDTMKDRELGEEAVLEKFGVRPDKVVDVQALAGDSVDNVPGVPGIGVKTGAQLITEYG
                     DLETLLARAPEIKQQKRRENLIAFAEQARLSKRLVTLDDKVPVDHDVGSFAVDTPRAS
                     DLIGFLKALEFTSFTKKISGELDADSSAIEPASIEIKFWPPEGQDVQPPTPHHDRAST
                     RSSSPSEVPDGDARVERRHDEKKVVEADEDFEKSLLAIPFVHANYETVSSLKDLDRWI
                     AEATELGAVAVDTETDSLDSMQAGLVGVSLSTAPGKACYIPLAHVGEGGGLFGSEKIT
                     GQIPLQEAIAHLKPLLENPGVLKIGQNLKYDLQVLRRYGTDIRPIDDTMLLSYAIESG
                     ENGHGMDEMSVKHLGHKPISFKEVAGSGKSMISFAQVPLDKATAYAAEDADVTLRLWK
                     LLKPRLLRKRKVTVYETLERPLIPVLQAMEHVGILVDRNVLAKLSNEFANGQGKIEAQ
                     IHELTGERFNIGSPKQLGDILFGKMALPGGRKTATGAWSTDSDALEDLAAQGIELARR
                     VVDWRQLAKLRSTYTDALPNYINPTTGRVHTSYAMAATSTGRLSSSDPNLQNIPVRTD
                     EGRRIRTAFIAPPGTKLISADYSQIELRVLAHVADIPQLKKAFADGLDIHAMTASEMF
                     GVPIVGMDSAVRRRAKAINFGIIYGISAFGLANQLSISREEAGD"
     gene            complement(2477..3550)
                     /locus_tag="PKV23_03580"
     CDS             complement(2477..3550)
                     /locus_tag="PKV23_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019199661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HQX84548.1"
                     /translation="MPVSHSRISWVDYSKGICIILVVMMHSTLGVENAAGGLSWLHDF
                     IGWAKPFRMPDFFLISGLFLASRINRPWREYFDSKVLHFAYFYILWMTIQFATKGYGL
                     YQANGVEGLLRDYALGFVEPFGTLWFIYMLAVFFVVVKLTRHVPPLYVFLAGALLEMA
                     PIASGHMVIDEFAARFVYFFAGYWLAGNVFKFAEGVNFKSVPAIVSGLFIWAIVNTVM
                     VRSGLSEVPGLGLGLGFVGAAAVISMGVLLSKARLAAPIRYFGENSIVIYLAFFLFMA
                     GARSVLLKYNVVADLGSVALLTTAAGVVGPILLFWATRHTKLSLLFRRPSWARLASRP
                     AQWHSDGHVTFNQPEARGPSLSR"
BASE COUNT          791 a         1136 c         1078 g          727 t
ORIGIN      
        1 atctcccgct tcctcgcggc tgatggacaa ctggttggca aggccgaaag ccgatatccc
       61 gtagatgatt ccgaaattga tcgccttggc gcgcctccgc accgcactgt ccatgccgac
      121 aatcggcacg ccaaacattt cgctcgccgt catcgcgtga atatctagcc cgtcggcgaa
      181 ggctttcttg agctgcggaa tatcggccac atgggccagc acgcgaagct cgatctggct
      241 gtagtcagcg ctgatcagct tggtgcctgg cggtgcgatg aatgcggtgc ggatgcggcg
      301 gccttcatcg gtgcgcaccg gaatgttctg caaattagga tcggatgagg agagccgccc
      361 cgtagaggtc gccgccatgg catagctggt gtgcacccgc cccgtggtgg gattgatgta
      421 attgggaagc gcatccgtgt aggtggaacg cagcttggca agctgccgcc aatccaccac
      481 gcggcgagca agctcaatgc cttgcgccgc caggtcttcc agcgcgtcgg aatcagtgct
      541 ccatgccccc gtcgccgtct tgcgcccgcc aggcaatgcc attttcccaa acaatatatc
      601 gcccaactgc ttgggcgagc cgatgttgaa gcgttcgccg gtgagttcat ggatctgcgc
      661 ctcgatcttg ccctggccat tggcaaactc attggagagc ttggccagca cattgcggtc
      721 caccagaatg cccacatgct ccatcgcttg cagaaccgga atgagcggcc gctccagcgt
      781 ttcataaacc gtgaccttgc gcttcctgag caggcgcggc ttgagcagct tccagagacg
      841 cagcgtcaca tcggcatcct ccgccgcata ggctgtggcc ttgtcgagcg gcacctgcgc
      901 gaacgagatc atggatttgc cggagcccgc cacctccttg aagctgatcg gcttgtggcc
      961 caggtgcttc accgacattt catccatgcc atggccgttc tcgcccgact cgatggcgta
     1021 agacagcagc atggtgtcgt cgatggggcg gatatccgtg ccgtagcgcc gcaaaacctg
     1081 caggtcatac ttcaggttct ggccgatttt caaaacgccg ggattttcca gcagtggctt
     1141 gagatgtgcg atcgcctctt gcaaagggat ctgtccggtg attttttccg aaccgaacag
     1201 cccgccgccc tcgcccacat gggccagcgg aatgtagcaa gccttgcccg gcgctgtaga
     1261 tagcgaaaca ccgactagcc ctgcctgcat ggaatcgagc gaatccgttt ccgtatcgac
     1321 cgccaccgcc ccaagttccg tcgcctcggc aatccagcgg tccaggtcct tcagactgct
     1381 gacagtctcg taattcgcgt gcacaaaggg aatggcgagc aagcttttct caaaatcctc
     1441 gtccgcttca accacctttt tctcatcatg cctgcgctcg acgcgggcat ctccgtctgg
     1501 cacttcgctt ggcgaagatg accgggtcga agcccggtca tgatgaggag tggggggctg
     1561 tacgtcttgg ccctcaggtg gccagaactt tatctcgata gatgccggct ctatcgcgga
     1621 actgtccgca tccagttcac cggaaatctt cttggtgaaa ctcgtgaact ccaaagcctt
     1681 caggaaaccg atgaggtcgg aagcccgtgg cgtatccacc gcgaaagaac ccacatcatg
     1741 gtccaccggc accttgtcat cgagcgtgac cagccgcttt gacagccgcg cctgttcggc
     1801 aaatgcgata aggttctccc ggcgcttctg ctgcttgatt tccggggccc gcgccagaag
     1861 cgtttcgaga tcgccgtatt cggtgatgag ttgcgccccg gttttgacgc cgatgccggg
     1921 aaccccgggc acattgtcaa cactgtcgcc ggccagcgcc tgcacatcga caaccttgtc
     1981 gggtctaacc ccaaatttct caaggaccgc ctcttcgccc agctcccggt ccttcatcgt
     2041 atcgagaagc gtgacgccct tgcgcaccag ttgcatcaag tccttgtccg atgacacgat
     2101 gcggcaggtg gcgcctgcct caaccgcctg tcgcgcatag gtggcaatca ggtcatcggc
     2161 ctcataattt gcctgttcga cgcaggccac attgaaggcc gtggtcgcct gcctgatcag
     2221 gccgaattgc gggcggaggt cggctggcac ttcgggccgg tgggccttgt attccggata
     2281 gatgtcattg cggaaggtct tcgacgattc atcgaaaatc actgcgagat gcgttggttt
     2341 ctcgttggct cgggtttcct tgagcagctt ccacagcatg ttgcagaagc cctgcacggc
     2401 ccccaccggc aggccgtcgc tcttgcgggt taagggcgga agggcatggt aggcgcggaa
     2461 aatatagccg gagccatcaa cgagatagag atggtccccg ggcttcaggc tggttgaatg
     2521 tgacatggcc gtcactatgc cattgggcag gccgcgatgc cagtcttgcc cacgaaggcc
     2581 tgcggaaaag cagggacagc tttgtatgtc gtgtggccca gaacagcagg ataggcccca
     2641 ccactccggc agcagttgtc agcagcgcca cactgccgag atcggccacc acgttatact
     2701 tgagcagcac actgcgcgcc cccgccatga acaggaagaa ggcgaggtag atgacgatgg
     2761 aattttcgcc gaagtagcgg ataggcgccg caagccgcgc cttggagagc agcacgccca
     2821 ttgaaatcac cgccgcggcg cccacgaagc cgaggcccag gccaaggccc ggcacttcgg
     2881 aaaggccgga gcgcaccatg acggtattga caatcgccca gatgaaaagc cccgagacga
     2941 tggccggaac cgatttgaag ttcacgccct ccgcgaattt gaaaacatta ccggcgagcc
     3001 agtagcccgc gaaaaaatag acaaagcgcg ccgcgaattc atcaatcacc atgtggccgc
     3061 tggcgatagg cgccatctca agcagggcgc ccgccaggaa gacatagagc ggcggcacat
     3121 ggcgcgtgag tttcaccacc acgaagaata ccgccagcat gtaaatgaac catagggtgc
     3181 cgaaaggctc cacaaagccc agggcataat ccctgagcag cccctcaacc ccatttgcct
     3241 ggtacaggcc atagcccttg gtggcaaact ggatggtcat ccacaggatg tagaaatagg
     3301 caaaatgcag caccttgctg tcgaaatatt cccgccatgg ccggttgatg cgcgaggcca
     3361 ggaacaggcc tgagatcagg aagaagtccg gcatgcggaa gggcttggcc cagccgatga
     3421 aatcatgcag ccaggagagc cctccggcgg cgttttccac ccccagggtg gaatgcatca
     3481 tcacgaccag aatgatgcaa attcccttgg aataatcgac ccaactgata cgggagtggc
     3541 tgacaggcat ggcaatatcc ttaacgtccc ggcatgggac acatgaggat ttccgccgca
     3601 aaaaccctgc cattgcgaaa ggcagcaggg ctgggctata gctggcccgt taacggccaa
     3661 agcacccgta gctcagatgg atagagcgct gccctccgaa ggcagaggtc acaggttcga
     3721 atcctgtcgg gt
//
LOCUS       DAORVN010000128         3725 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_19999_length_3725_cov_3.337602, whole genome shotgun sequence.
ACCESSION   DAORVN010000128 DAORVN010000000
VERSION     DAORVN010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3725)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3725)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3725
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19999_length_3725_cov_3.337602"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..528)
                     /gene="modA"
                     /locus_tag="PKV23_03585"
     CDS             complement(<1..528)
                     /gene="modA"
                     /locus_tag="PKV23_03585"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HQX84549.1"
                     /translation="MFSISRYFCVVVIAFLSMAFHAHAENVNVFAAASMKTALDDAIR
                     LWKTQSGKEVVATYGSSGTLARQIAAAAPADIFISADLAWVDDIAKKSLIKPDSRRNL
                     VGNTLVLVAAAGTGLKIGLEKESNLAASLGIEKLAVGDVKSVPAGKYAKSALENLGLW
                     ASVEPNLVMQENVRSA"
     gene            complement(610..1191)
                     /locus_tag="PKV23_03590"
     CDS             complement(610..1191)
                     /locus_tag="PKV23_03590"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HQX84550.1"
                     /translation="MAGAPLFSAEKCALIVIDVQDYFLAKLPHGWRTPLVERIAWLMQ
                     VASLLDIPVIATAEDIASDGPLVPALEKQLRAGASVHDKKVFGLSGQADILDAVRGTG
                     RSECVVVGLETDVCIAHSALGLIDQGFRVAAIDDATASPPPHHEAGLRRMAAGGVVLT
                     NTKGIYYEWVRDLATHARVKSRINLPLPPGLTL"
     gene            complement(1202..2827)
                     /locus_tag="PKV23_03595"
     CDS             complement(1202..2827)
                     /locus_tag="PKV23_03595"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF039309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HQX84551.1"
                     /translation="MQGHGKISDYRQTEPVRVQREAIYLDQLEANLDDPFHDEPIHHR
                     WLLTTCIAGIIGTLVVGGAALGLFGENAVPGDAFAAVDRSPETGTKSRLAKLASLSQS
                     TEVIPERFLGGDNNYPEISSGDLPYGDGKTIVLDAEIQASETESGNITIITKTPPAEP
                     IDETFRIAKGRTLVDELTNRGVTKDAAQALASAMEPVFPSHMIMAGTEFELTLDRQQD
                     FYGRYATFPVQLSFKPGPKETITIEADEDGRFVARVDGKEKGMASQYAEITQFRTKAK
                     VGSSLYAAAKDNRVPEYIIAELTRMFSYDVDFQRQVKPNDTFEVFYGNPLTGSSTKRK
                     VLHYAQLTLSGVTKTYYRYVNADGQVDYLDDNGKSASKALLKTPVAGSKLTSGFGMRK
                     HPLLGYTKMHTGVDFGVPYGTPIRSAGAGVVEIAGRHGAYGVTVEIKHSNKFETLYAH
                     MSKLADGIRTGKKVNQGQIIGYVGATGRATGPHLHYEVRVGGRPMNPMKVKATGGKQL
                     AGKDLQKYKHYKTRVLAMMQNAPSATQVAQAGQ"
BASE COUNT          755 a          988 c         1140 g          842 t
ORIGIN      
        1 ggcgctgcgg acgttttcct gcatgacaag gtttggctca acgctggccc aaaggcccag
       61 attttccaaa gcagatttgg cgtattttcc ggcgggcacg gatttcacgt cgcccaccgc
      121 cagtttctcg attccgagcg aggcagcaag gttggactcc ttttcaaggc ctattttcag
      181 gcccgttccg gctgccgcca ccaacaccag agtattgcca accaggtttc tccgggagtc
      241 cggcttgatc agacttttct ttgcgatatc atccacccag gcaaggtcgg cggagatgaa
      301 gatgtcggcg ggggcggctg cggcgatctg cctggcaagc gtgccagagg agccataggt
      361 cgccacaact tcctttccag attgggtttt ccaaagcctg atggcatcat caagggcagt
      421 cttcatgctg gcggcggcga agacattgac gttttcggca tgggcatgga aagccatgct
      481 caagaaggcg atgacgacaa cgcaaaaata gcggctgata ctgaacatcc ctgatcctct
      541 ggcatatcgt tatgttctat tggatataac gatagagaaa tgaagtcaaa cccgatgttg
      601 aagtgcggtt tacagggtga ggccgggtgg caaggggagg ttgatccggg atttcacgcg
      661 ggcatgggtt gcgaggtcgc ggacccattc atagtagatg cccttggtat tggtgagcac
      721 aacgccgcct gccgccatgc ggcgaaggcc ggcttcatgg tgtggcggag gcgatgcggt
      781 ggcatcgtcg atggcggcca cgcggaatcc ctggtcgatc agcccgagcg ccgaatgagc
      841 aatgcagacg tcagtttcca gtcccaccac aacacattcg ctgcggcccg tgccgcggac
      901 ggcgtcaagg atgtcagcct ggccgctaag gccgaagact ttcttgtcgt gcacgcttgc
      961 cccggcgcgc aattgttttt cgagcgccgg caccagcgga ccgtcgctgg ctatgtcctc
     1021 cgccgtggcg ataaccggga tgtcgagaag gctggccacc tgcatgagcc aggcgatgcg
     1081 ctcaaccagc ggtgtgcgcc agccgtgagg gagttttgcc agaaaatagt cctgaacatc
     1141 gatgacgatg agggcgcatt tttcggccga gaacagggga gctccggcca ttgccgcagg
     1201 cttactgtcc ggcctgggca acctgggtgg ccgacggggc gttttgcatc atggccagca
     1261 cacgcgtctt gtaatgcttg tacttttgca ggtccttgcc ggccaattgc ttgccgccgg
     1321 tggccttgac cttcatcggg ttcatcgggc gcccgccaac gcggacttca taatgcaggt
     1381 ggggcccggt ggcgcggccg gtggcgccga catagccgat gatctggccc tggttgactt
     1441 tcttgccagt gcggatgcca tcggccagct tgctcatatg ggcatagagc gtctcaaact
     1501 tgttgctgtg cttgatttcg acggtgacgc cataggcacc gtggcggccg gcaatttcaa
     1561 ccacgccggc accggccgag cggatggggg tgccataggg aacgccaaaa tccacgccgg
     1621 tgtgcatttt ggtgtagccg agcaggggat gcttgcgcat gccaaagccg gaggtgagct
     1681 ttgaaccggc gaccggggtt ttgagaaggg ccttggaggc gctcttgccg ttgtcgtcca
     1741 ggtaatccac ctggccgtcg gcattcacat agcggtaata ggttttggtc acgccgctga
     1801 gggtcaactg ggcgtagtgc agaactttgc gcttagtcga tgaaccggtg agcgggttac
     1861 cgtagaaaac ctcaaaggtg tcgttcggct tgacctggcg ctggaagtcc acgtcgtagg
     1921 agaacatgcg ggtcagctcg gcaatgatat attccggaac ccggttgtcc ttggccgcgg
     1981 catagaggct ggagcctacc ttcgccttgg tgcggaactg ggtgatctcg gcgtattggg
     2041 aagccatccc cttttccttg ccatcaaccc gggcgacaaa gcggccgtct tcgtcggctt
     2101 cgatggtgat ggtttccttg gggcctggct tgaaggaaag ctgaaccggg aacgtggcat
     2161 aacggccata gaagtcctgc tggcggtcaa gggtcagttc aaattcggtg ccggccatga
     2221 tcatgtggga cggaaatacc ggctccatgg ccgaagccag ggcctgcgct gcatccttgg
     2281 tcacgccgcg gttggtgagt tcgtccacca gagtgcggcc cttggcgatc ctgaaggttt
     2341 cgtcgatggg ttcggccggc ggtgtcttgg tgatgatggt gatgttgccg gattcggttt
     2401 cggaagcctg tatttccgcg tccagcacaa tggtcttgcc gtcgccatag ggcaaatcac
     2461 cggagcttat ctccggatag ttgttgtcgc cgccgagaaa gcgctcggga atgacttcgg
     2521 tgctctggga cagggaggca agctttgcca gcctcgattt agtgccggtt tcgggggatc
     2581 tgtccacggc ggcaaaggca tcgccgggca cggcattttc accaaacagc cccaaggcag
     2641 cgccgcccac aaccagggtc ccgataatcc cggcaatgca ggtggtcagc agccagcgat
     2701 gatggatagg ttcgtcgtga aagggatcgt caagatttgc ttcgagctgg tccagataaa
     2761 tggcttcgcg ctgaacgcga acaggttcag tttgccggta atcggatatt ttaccgtggc
     2821 cctgcaatat cgatgtcccc tcgcactaaa tactaacgtc ggttcttttg tttgggcccc
     2881 acagcctttt tcaaggaaat tggcccaaat ccatacgatc tgtcaatctt tagacacccc
     2941 gccttgcgac tgggggaatt ggctttcccc catagagtac ctggttcgac ctgtcccact
     3001 tatcgaaccc gggaatcatt gccacccaac tgttacaggt tggttaacta tgacagcaat
     3061 cgtgctcaat gatggcgaga ttggggcgcc ggggcatgga aaatgcgtcc gcggttcggt
     3121 tgtgggtgaa atcgccagca gaacagcaga cgggtcgggc aaaaatattt cccgttgcga
     3181 ttccgggtca ggttctgccc accgggcagc gcgtgaaatc catataatcc ggggcttcgt
     3241 tgtgtttgca aaaattttct aacaaacgct tgacacttcc gcccgccccc tcctataaag
     3301 cggccactga gggacgcggc gttgcaaaca actgccgacg ttcctgtgtg aattcaatgt
     3361 gcagctggct gagacgccgg tgttacccga aaggggaacc ggtggatttt tcgtcggctg
     3421 catgaatcgg aggcttaata gaagcttccc cgctgtttga catcgttgaa gaaagaaaga
     3481 gaaacgcagg cggcgtgtgt cctggcggtt cgcgaaggca acttcgtgga caaccgaaac
     3541 attagtcgta tgcgttttat atttaaagag aagacaatgc tttggtggaa acactagagc
     3601 acatgatctc tcgtcaattt agtacgcaat gcgatcattt ttctggtgga tttatccatc
     3661 agaagagctg tttcaaactt gagagtttga tcctggctca gaacgaacgc tggcggcagg
     3721 cttaa
//
LOCUS       DAORVN010000129         3720 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_20051_length_3720_cov_3.415825, whole genome shotgun sequence.
ACCESSION   DAORVN010000129 DAORVN010000000
VERSION     DAORVN010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3720)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3720)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3720
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20051_length_3720_cov_3.415825"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(153..497)
                     /locus_tag="PKV23_03600"
     CDS             complement(153..497)
                     /locus_tag="PKV23_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018389297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HQX84552.1"
                     /translation="MSIPLGTAQALKLWHDVSLAQVHGDEADLSTRQVSILLTIYMEA
                     PPHTIRDLARKLHVSKPVITRALDSMGKLDLVARRRDTADRRNVLVQRTVKGALYLDR
                     LADLIGKNSKNL"
     gene            complement(562..1941)
                     /locus_tag="PKV23_03605"
     CDS             complement(562..1941)
                     /locus_tag="PKV23_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008964740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase family protein"
                     /protein_id="HQX84553.1"
                     /translation="MDARKILLKAGDGRAVKIQVSTAASWPKLRSRLSRLAQTWADAQ
                     NFAGNAGTLLQIPDGKGAVAEVILGSNPEDDGFRLARLYGKLPPGTYRFGEAKSETPE
                     YAELAWCLEAYGFDRYKTGPKKFPSLVCSPHTDYGNVARLANATYLVRDLINTPSNDF
                     GPDELEAAARKIAAQYKAKFSVVKGGALKRGFPMVYAVGKASPRQPRLIDFTWGAVRA
                     PRVTLVGKGVCFDTGGLDIKSASGMVLMKKDMGGAANVLGLAQMIMDAQLPVRLRVLI
                     PAVENAIAGNAFRPGDILKSRKGLTVEIGNTDAEGRLVLGDALALADEETPDLLIDMA
                     TLTGSARVALGPDLPPFYTNDEKLAGDVTRHSQTQKDPLWRMPFWDPYYSLFESPVAD
                     LNNSADTSFAGSITAALFLKRFVENAKSYLHLDIYGWTPTAKPGFPKGGEAQGIRTLF
                     SLISERYKK"
     gene            2094..2864
                     /locus_tag="PKV23_03610"
     CDS             2094..2864
                     /locus_tag="PKV23_03610"
                     /inference="COORDINATES: protein motif:HMM:NF019339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQX84554.1"
                     /translation="MRRITLPLLLVVSAFAISACQSKTASLESLDGLTTASTEPASFK
                     KTAELGNRWQGDPKNLELGLAYADGLEKLGQTDQQLEVLAKLSAQNPEKSHIQVLLGK
                     KLLAAGRSGQAIPVLKAVVDGGTADWRTLSALGSAYDQQAQYGTAREFYQKALALQPN
                     QISVLNNMGMSHALEGNLKQAEETLRSAMTLPDGKSMPRLRQNLALVVGLQGRFEESR
                     KIASEDLPPEEVEANMAYLQKMLSQPNTWQQLSDDTQG"
     gene            complement(2933..>3720)
                     /locus_tag="PKV23_03615"
     CDS             complement(2933..>3720)
                     /locus_tag="PKV23_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007199647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HQX84555.1"
                     /translation="VSGDARLRERPKRSLVTQIVESLNLRSVFEAEASREALRQAGMR
                     SERHLVAFLALRLITPFIVAIVVFIYSSTVFADRIPPQMRLVATVVGLVFGFYLPSIV
                     LKNLKTRRQDSIRKAWSDALDLLLICVESGMAIEPALQRVAREIGSGSVALAEELTLT
                     VAELSYLQDRRKAFENLGKRTGLPTVKSVVTSLIQSERYGTPLGTALRVLAQENRDAR
                     MQEAERKAAALPPKLTVPMILFFLPVIFVIILGPSVIMVMKLQ"
BASE COUNT          805 a         1066 c         1108 g          741 t
ORIGIN      
        1 cgttatcgaa gcgtcccggt gcaacgctgc aaccggggtg ataacttcca tgaccacgcc
       61 ttcaacaaat ttcttcgcat cgacacggcc ctgcaatttt acatcggcaa gatcggggcg
      121 gaaggcatgg agcctggaat cgagatcagc catcataggt ttttcgagtt cttgccaatc
      181 aggtcggcca gccggtcaag atacaaagca ccctttacag ttcgctggac caatacattg
      241 cggcggtcgg cggtgtcgcg gcggcgcgcc acgaggtcga gcttgcccat cgaatccagg
      301 gcccgggtga tcacgggttt tgagacatgg agcttccggg cgagatcgcg aatggtgtgc
      361 ggcggggctt ccatgtaaat cgtcagcaga atgctcacct gcctggtgga aagatcagcc
      421 tcatcgccgt gcacctgggc aaggctcaca tcgtgccaaa gtttgagggc ctgcgcggtt
      481 cccagcggaa ttgacatctt gcgattcggc aatcgttacg gatgcgtttc attctcccgc
      541 atcgggccac aaggtgcaag cctatttctt gtaacgttcg gaaatcaatg aaaacagggt
      601 tcttatgccc tgcgcctcgc cgcctttcgg gaagcccggc ttggccgtcg gggtccagcc
      661 ataaatatcc agatgcagat aggatttggc attctcgaca aaacgcttga ggaaaagggc
      721 tgcggttatg gagccggcaa agcttgtgtc ggcactgttg ttcaggtcgg caacgggcga
      781 ttcaaacagg gaatagtaag ggtcccaaaa cggcatgcgc cagagggggt ccttctgagt
      841 ttgggaatgc cttgtgacgt cgcccgcgag tttttcgtca ttggtgtaga agggcggcag
      901 gtcaggcccg agggcgacgc gggccgagcc cgtgagggtt gccatgtcga tcagcagatc
      961 gggtgtttcc tcgtcggcca gggccagggc atctcccaga accaaacggc cttccgcatc
     1021 ggtattgccg atctccacgg tcagaccttt acggcttttc aggatatcgc cggggcggaa
     1081 ggcattgccg gcaatggcgt tttccacggc aggaatgagg acgcgcaggc gcacgggcaa
     1141 ctgcgcgtcc atgatcatct gggcgagccc gaggacattg gcggcaccgc ccatatcctt
     1201 tttcatcaga accatgccag acgccgactt gatatcaagg ccgcccgtat cgaagcagac
     1261 ccctttgccg acgagcgtta cccggggagc ccgcaccgcg ccccaggtaa aatcaatgag
     1321 gcggggttgc ctggggcttg ccttgccgac cgcataaacc atcgggaagc cgcgcttcag
     1381 ggccccacct tttacaacgc tgaattttgc cttgtattgg gcggcaatct tgcgggcagc
     1441 ggcttccagt tcgtcggggc caaaatcatt ggagggcgtg ttgataaggt cgcgcaccag
     1501 ataggtggcg ttggccaggc gtgcgacgtt cccataatca gtatggggag agcatacgag
     1561 gctggggaat tttttgggac ctgtcttgta gcgatcaaaa ccataggcct cgaggcacca
     1621 ggccagttcg gcatattcag gagtctcaga tttcgcctcg ccgaacctat atgttcccgg
     1681 cggaagcttt ccatagaggc gggccagacg gaaaccgtca tcctccggat tagaacccag
     1741 gatgacctcg gcaacagcac cctttccatc agggatctgc agaagagttc cggcgtttcc
     1801 ggcaaaattc tgggcatcgg cccaagtctg ggcgaggcgc gaaagccggc tccggagttt
     1861 tggccagctt gccgccgtcg acacctgaat tttcacggcc ctgccgtctc cggccttcag
     1921 cagtatcttt cgggcatcca tcaacacacc ttctttcgcg agcaaccccg gcctcaggcc
     1981 cggcatggtt aattgtttat tgacacctgc ataccatgat cggcctaagt ccttttcgca
     2041 atatgcatga ttctattggg gtttgagtga tggtgtcggt caatactgga aatttgcgca
     2101 ggatcacact gcccttgctc ctcgtggtgt cagccttcgc aatttcggcc tgccagagca
     2161 agacggcaag cctggaaagc cttgacggcc tgaccacggc cagcacggaa ccggcatcct
     2221 tcaaaaaaac ggccgaactc ggcaaccgct ggcaaggcga cccgaaaaac cttgaattgg
     2281 ggttggccta tgccgacggg ctggaaaagc tgggccagac ggaccagcag ttggaagttc
     2341 ttgcaaagct atcggcccag aatccggaga agagccatat ccaggtgctc ctgggcaaaa
     2401 aactcctggc tgccgggcgg tcaggccagg cgattccagt tctcaaagcg gtggtcgacg
     2461 gcggcacggc ggactggcgc acgctgtcgg cgctgggttc agcttatgac cagcaggcgc
     2521 agtatggcac ggcgcgggaa ttctaccaga aagccctggc gcttcagccc aatcagattt
     2581 ccgtgctcaa caacatggga atgtcccatg cgctggaagg caacctgaag caggcggagg
     2641 aaaccctgcg cagcgccatg accctgccgg acggcaagtc gatgccaagg ctgcggcaga
     2701 accttgccct ggtggttggc ctgcaagggc gctttgaaga atcccggaaa atcgccagtg
     2761 aggaccttcc gccggaagag gtcgaggcaa acatggccta tctgcaaaaa atgctgtcgc
     2821 agcccaacac ctggcagcag ctctcggacg acacccaggg ctaaacaccg tccaggccaa
     2881 atgacaaagg cgcgaatgtt ttcgcgcctt tttctctggc atcatggcat tgctattgaa
     2941 gcttcatgac catgatgacg gatggtccca gaatgatgac gaagatgact ggcaggaaga
     3001 acaggatcat gggcacggtc agcttgggcg gaagtgccgc cgccttgcgc tcggcttcct
     3061 gcatgcgggc atcacggttt tcctgggcca gaacccgcag ggccgtgccc aagggcgtgc
     3121 cgtagcgctc cgactgaatg agactggtga caaccgattt gactgtcggc aggccggtgc
     3181 gcttgcccaa attctcaaat gcttttcggc gatcctgcaa ataggagagc tcggccaccg
     3241 tcagggtcag ctcttcagcc agggctaccg agccgctgcc gatttcgcgc gccacacgct
     3301 gcagggcagg ctcgatcgcc atgccggatt ccacgcaaat cagcagaaga tcgagcgcat
     3361 ccgaccaggc tttcctgatt gagtcctgcc gccttgtctt gaggttcttg agcacgatgc
     3421 tgggcaggta aaagccaaac accaaaccaa caacagttgc aaccagccgc atctggggcg
     3481 gaatacggtc ggcgaatact gtcgacgaat agatgaatac gacgatggcg acgataaaag
     3541 gcgtaatcag gcgcagcgcg agaaatgcca cgaggtggcg ttcactgcgc atgccggcct
     3601 ggcgcagcgc ttcgcgcgat gcttcggcct caaacacgct gcgcaaattg agggattcaa
     3661 caatctgcgt aacgaggctg cgcttcggcc tttcccggag cctggcatca ccgctcacca
//
LOCUS       DAORVN010000130         3715 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_20107_length_3715_cov_2.891530, whole genome shotgun sequence.
ACCESSION   DAORVN010000130 DAORVN010000000
VERSION     DAORVN010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3715
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20107_length_3715_cov_2.891530"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..946
                     /locus_tag="PKV23_03620"
     CDS             <1..946
                     /locus_tag="PKV23_03620"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HQX84556.1"
                     /translation="DHGHPVPVRRDGGLVLVRLLQRLPLGSGFEKQFGAPYRVAHRAD
                     LHNALLESARSKPGLEFQNNAEVTGISVAETTLSLKSGKELNGAAIIAADGVRSGIRK
                     QLLGQPGKSPIGHLLHRGLMPIASVPASVNVDVVTLWLFPGGHVVHYAVSNWRHFNIV
                     AAVEDPDVSLWTAFQGACQPLADILEQKTDWTKWPALDFAPARNWSRNRTVLVGDAAH
                     ASLPYLAQGAAMALEDACVLSAKLGDRSDIPSAFAEFSELRFSRASAIQKKSRQLGRI
                     YHAGGVLRRARNAALIAMPLRRFTRQVAWIYDWKPEGK"
     gene            complement(990..1358)
                     /locus_tag="PKV23_03625"
     CDS             complement(990..1358)
                     /locus_tag="PKV23_03625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX84557.1"
                     /translation="MRTFNSTNSFAAAAFGAAALFSMLSFGSSAEAAQNLSQCYGKST
                     GSVVSCCEKLTKSQRPLWMTQSGTNCREAVVCGGLQSKDRRCYVRIVLIDPQRGDTPG
                     GTPDRGDRGRDSRGGVQSAK"
     gene            1536..2078
                     /locus_tag="PKV23_03630"
     CDS             1536..2078
                     /locus_tag="PKV23_03630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX84558.1"
                     /translation="MKKLMFLSLAAFLMASASSLSFAAGSGGGEPVEAPKCKKGEVLK
                     KVGNVKKCVKVESGILPDEDLYQQGRALAKAGEYEWALQVLAAIENQNDPRVLNYTGY
                     SNRKAGRLEIGITYYRKALAIDPNFVLAREYLGEGYVAAGRIDLAKLELNEIKARAGT
                     GSEEYRDLAKAIDTGVVATD"
     gene            complement(2135..2545)
                     /locus_tag="PKV23_03635"
     CDS             complement(2135..2545)
                     /locus_tag="PKV23_03635"
                     /inference="COORDINATES: protein motif:HMM:NF019501.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BA14K family protein"
                     /protein_id="HQX84559.1"
                     /translation="MRIFQVLAGVAALSCISAPAFADAKSYCEIFGQDFASAKTSNVD
                     EWQLTYRNAFSNCMAQYTSEPAPAPPVKKTAEKASKKIVVVPVTSTSTKKRTRILAPG
                     STAWISACAAKYSSFDSGTGNYKSSSGKIRRCNL"
     gene            2767..3096
                     /locus_tag="PKV23_03640"
     CDS             2767..3096
                     /locus_tag="PKV23_03640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX84560.1"
                     /translation="MASAAGQKPLILPIADADQERADARAEARARLTKGQAHVPGTGF
                     GSLVLYTVVALALLASGYLWFSGNANTIMLFPTVSETVPSPDINADVPPAPQAAPPPA
                     AAPATNP"
     gene            complement(3181..3570)
                     /locus_tag="PKV23_03645"
     CDS             complement(3181..3570)
                     /locus_tag="PKV23_03645"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HQX84561.1"
                     /translation="MIKTFNPSGMVQPASRYSQGVEVSGTVRWLHIAGQIGVAPDGTV
                     AKGLEAQFRQAFENIGTVLAAAGMTKADLVKVTVFVTVPDLETVGIYRKARDQWMEGH
                     APAATYLVVAGLAHRDYLVEIEAIAAG"
BASE COUNT          746 a         1177 c         1029 g          763 t
ORIGIN      
        1 cgaccacggc cacccggtgc ccgtccgtcg tgatggcggg ctcgtccttg tgcgactcct
       61 ccagcgcctt ccgctcggat cggggttcga aaagcagttc ggtgccccct accgtgtcgc
      121 ccaccgcgcc gatttgcaca atgccctcct cgaatcggcg cgttccaagc ccggcctcga
      181 atttcagaac aatgccgaag tcacaggaat ttcggtggcg gaaaccaccc tgtcgctgaa
      241 atcgggaaaa gaactcaacg gcgcggccat tatcgccgcc gacggcgtgc gttccggaat
      301 tcgaaagcag ttgctcgggc agcccggaaa aagcccgatt ggccacttgt tgcaccgggg
      361 ccttatgccc attgcgtcag ttccggcaag cgtcaatgtc gacgttgtga ccctctggct
      421 ttttcccggc ggccatgttg tccactacgc cgtcagcaat tggcgtcatt tcaatattgt
      481 tgcggcagtt gaggatcctg atgtgtccct ctggaccgcc tttcagggtg cctgccagcc
      541 gcttgcggac atactcgaac agaaaacaga ttggaccaaa tggcctgccc tcgattttgc
      601 tccggcgcga aactggagca ggaaccgcac ggtgcttgtg ggtgacgccg cccacgccag
      661 cctgccctat ctcgcccagg gtgccgccat ggcccttgag gatgcctgcg tgctgtcagc
      721 caaactcggc gatcgcagcg atattccttc agccttcgcg gagttttctg agctgcggtt
      781 ttcgcgcgcc tcggccatcc aaaaaaagtc ccgccagctc ggccggattt atcatgccgg
      841 cggcgtcctg agacgggccc gcaacgccgc tctgatagcc atgcccctgc gccgcttcac
      901 cagacaggtg gcatggatct atgattggaa accagaggga aagtgaccgc tcgcgagatc
      961 acctcccatt ggcttaaacg aattggcgat tacttggccg actgaacgcc accgcgcgaa
     1021 tcacggcccc gatcaccgcg gtccggagtc ccgcctgggg tgtcaccgcg ctgcgggtcg
     1081 atcagaacga tgcgcacgta gcaacggcga tccttgcttt gcaggccgcc gcaaaccacg
     1141 gcttcccggc agttcgttcc ggattgagtc atccacagtg ggcgctgtga cttcgtcagt
     1201 ttttcgcagc aggatacgac cgatcccgtt gacttgccgt agcactgtga gaggttttgc
     1261 gccgcttcag cgctgctgcc gaatgaaagc atgctgaaaa gggcggcggc gccgaacgcg
     1321 gcggcggcga agctattggt tgaattgaac gtgcgcataa ttctctcctt gggttgcgat
     1381 cttgtttgcc cgttgcccca catttggcgc aatttgcctg agccccgcct gaatgcgccg
     1441 ttcatttttc gttaatctga aatccaatgc cccggcggcg ccgtaacagg gtaaagttcg
     1501 gctatattcc aaccccatag gcatggagat cgacaatgaa aaagctgatg tttctttccc
     1561 tggcggcttt tctgatggcc tcggccagct cgttgagctt tgccgccggc agcggcggcg
     1621 gtgagccagt tgaggctccc aaatgcaaga aaggtgaagt tctcaagaag gttggcaacg
     1681 tcaagaaatg cgtgaaggtt gaatccggca ttctcccgga cgaggatctc tatcagcagg
     1741 gccgcgccct ggccaaggcc ggtgaatatg aatgggccct gcaggtgctc gccgccatcg
     1801 aaaaccagaa tgatccccgc gtcctgaatt acaccggcta cagcaaccgc aaggccgggc
     1861 gccttgaaat cggcatcacc tattatcgca aggcgctggc catcgatccg aatttcgtgc
     1921 tggcccgcga atacctcggt gaaggctatg tcgccgccgg ccgcattgat ctggcgaaat
     1981 tggaactcaa tgagatcaaa gcccgcgccg gaaccggcag cgaagaatac cgcgacctgg
     2041 caaaggctat tgacacgggc gtggtcgcaa ctgactgatc aagtcaaggg ggtgcgcgta
     2101 cgccgcgtgc ccccgatttg tttaatcaat tgacctagag gttgcagcgc ctgatcttgc
     2161 cgctgctcga tttgtagttg cccgtcccgc tgtcaaacga cgaatacttt gccgcgcagg
     2221 ccgaaatcca tgcagtagat cccggcgcca ggatccgcgt gcgtttcttc gtcgaagttg
     2281 aagtcaccgg caccaccacg attttcttcg aagccttctc cgccgtcttc ttgaccggag
     2341 gcgcaggcgc aggttcgctg gtgtactggg ccatgcaatt gctgaaggcg ttgcggtaag
     2401 tgagctgcca ttcatccacg ttggacgttt tggcgctcgc gaaatcctgg ccaaagatct
     2461 cgcaatagga ttttgcatcc gcaaaagccg gagctgagat acagctcaat gccgccacac
     2521 ctgcaagaac ctggaatata cgcatgacac ttctaccttg gattgccgtt caggaacaat
     2581 cgcgccaaac tatggcagac ccggggcaga tcatagggca atttcctggg ctcgtaaact
     2641 cttgatatgt ctggctatag gtccttggca ccacggcggg cggcaacccc gggccgggaa
     2701 tggatgcgga acatctgcgc ccttcccgcg ttatcccagc aagatcaaac ctgaaaggat
     2761 atagccatgg caagtgctgc tggccaaaag ccactcatcc tgcccatagc cgacgcagac
     2821 caggaacgcg ccgatgcccg ggccgaagcc agggcccgct tgaccaaggg gcaagcccat
     2881 gtgccaggaa cggggttcgg aagcttggtc ctctatacag ttgttgcact tgccctgctg
     2941 gccagcggct acctgtggtt cagcggcaac gccaacacca tcatgctgtt tcccaccgtc
     3001 agtgaaacag tgccatcgcc ggatatcaat gctgatgtgc ctccggcgcc gcaagccgct
     3061 ccgccgcctg ctgccgctcc tgctaccaat ccatagattg gcgataggca gttcttagcg
     3121 ccaggcgccg gcaggaaaaa gcctccggac gcgggggctt tttctccgtg ggcgaaatcc
     3181 ctagccggca gcgatggctt cgatctcgac cagataatcc cggtgcgcca gccctgcgac
     3241 caccagatag gttgccgccg gcgcatgccc ttccatccac tggtcccgcg ccttgcggta
     3301 aatgcccacc gtttcaagat ccggcaccgt cacgaacacc gtcaccttca ccagatcggc
     3361 tttcgtcatg cccgccgccg ccagcaccgt gccgatattc tcaaaggcct gcctgaattg
     3421 cgcttcaagc cccttggcca ccgtgccatc cggagcaacc ccgatctgcc ccgcgatatg
     3481 cagccagcgc acagtcccgc taacttcaac accctggctg taacgcgacg ccggctgcac
     3541 catgccagat ggattgaatg tttttatcat ctaagtctcc tcatgttcta ggcggcacca
     3601 gccactcaac cacgccttgc ccccaggttc caatgccaag gcgcgccgcc agcgccggca
     3661 tgatcacttt cagttcatct tcaagtcccc agggcgaatt gacgatgatc aaccc
//
LOCUS       DAORVN010000131         3696 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_20287_length_3696_cov_3.549574, whole genome shotgun sequence.
ACCESSION   DAORVN010000131 DAORVN010000000
VERSION     DAORVN010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3696
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20287_length_3696_cov_3.549574"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            52..720
                     /locus_tag="PKV23_03650"
     CDS             52..720
                     /locus_tag="PKV23_03650"
                     /inference="COORDINATES: protein motif:HMM:NF021492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQX84562.1"
                     /translation="MELVAVSEESLFREVDEELRQEQYKKLWDKFGTYFVALCVVIVA
                     AVAGFKGYEYYKVKQSQAAAVVYFDGVKRATDGKFDDALTALAAVNHPGFKQLGLLQQ
                     ASILAEQGKTTEAVAAYDALAADASVDPVLRDVARIRAGYLLADSLKPDELISRLGSF
                     DRDGQVWRHAAREIFGLAAFRTGDYSMADRYMNANFADPETPQDMRQRAQVMIQLLTP
                     LLQK"
     gene            726..998
                     /locus_tag="PKV23_03655"
     CDS             726..998
                     /locus_tag="PKV23_03655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX84563.1"
                     /translation="MKKLPTFVIMAVLASSLMACSTVKRFTGEQDNSVLPGTREEILT
                     PDQQTARDPEVTGNKTTPCNPDDIDCITPSSETDSSEIDQESSTAQ"
     gene            995..2347
                     /gene="der"
                     /locus_tag="PKV23_03660"
     CDS             995..2347
                     /gene="der"
                     /locus_tag="PKV23_03660"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009491040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HQX84564.1"
                     /translation="MTFTVAILGRPNVGKSTLFNRLVGRKIALVDDQPGVTRDRREGL
                     AQIGDLSFRVFDTAGLDDAKKETLEARMSAQAETAVQDADLVFFVIDARAGITPIDRK
                     FAEKIRKFGKPVRVVANKAEARMAEAGVLESYSLGFGEPLAISAEHGEGMESLYDVIA
                     PLAPTTEEASDEELEEAELTRPLRLAIIGQPNAGKSTLVNTMLGEERMLTGPEAGITR
                     DAISTDWTFGGREIKLWDTAGIRRKSRVTGKIEKLAVSDALRAIRFADCVVVLIDASM
                     EIERQDLALSDLVATEGRAVVLALSKWDLVEDKQKVLAKVHSELEDVLPEIRGVPVVT
                     ISAKQERGVDKMMKAVFEAVDRWSARITTSQLNRWLEAAISKNPPPAPSGRRIKIRYA
                     TQASSRPPTFAVFGNQMAKMPESYKRYLMNTLRKDFDLAGVPIRFSLRGGKNPFDKDK
                     "
     gene            complement(2358..3125)
                     /locus_tag="PKV23_03665"
     CDS             complement(2358..3125)
                     /locus_tag="PKV23_03665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX84565.1"
                     /translation="MSQASIIDALRAARLSGEKLASYPGPAPASMAEAFAIQTAVRTT
                     IGWTLAGWKIGCTSERAQKALHTDGPFPGPLYRERIYGAGAHVETLASNSRTTEPEVA
                     FTLARDLPARDKPWSVEEVLEAVATVHPSIEIVNPRLPKGFNDVVEWYVADGGLSHAL
                     VLGPGVKPLARSDYARITNRISINGISKYTGIASNALGGPELALTWLANNLIASGLFL
                     REGDVVTTGVITDVFETQIGDFVKASYDLIGDVSVQL"
     gene            complement(3122..>3696)
                     /locus_tag="PKV23_03670"
     CDS             complement(3122..>3696)
                     /locus_tag="PKV23_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009491064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HQX84566.1"
                     /translation="GGSATLVPSDIKDFAAIDRLGAAIFERWKKLDILIGNAGILGKL
                     TPLAHLDPKVFDDVMAVNVTANYRLIRSLDPLLRASDAGRAVFVTSGLAYKCWAYWGS
                     YSISKAALEAMVRTYAAENGQTAIRANCFSPGATRTKMRAQAMPGEDPESLPSAEDVA
                     AQVIPMCLPAFTDNGAVYKYAPTGLFKQVL"
BASE COUNT          771 a         1032 c         1193 g          700 t
ORIGIN      
        1 gaatttgcct tgatttagcc cttccactgg cttaccccca cggcagcatt tatggagctg
       61 gtggccgtga gcgaagaatc cctattccgc gaggtggatg aagaactccg ccaggaacaa
      121 tacaaaaagc tttgggacaa atttggcact tactttgttg ccctgtgcgt tgtcattgtc
      181 gccgcggtgg cggggttcaa gggttatgag tattacaagg tcaagcagtc gcaagcggcg
      241 gcagtggttt attttgatgg cgtgaagcgc gccaccgacg gcaaattcga cgatgccctg
      301 acggcgctgg ccgcggtcaa tcatccgggc ttcaagcagc ttgggctttt gcagcaggcc
      361 agcattctgg cggagcaggg aaagacaaca gaagccgtgg ccgcctatga cgcgcttgcg
      421 gctgacgctt cggttgatcc agtcctgcgc gatgtggcgc ggattcgcgc cggttacctg
      481 ctggctgaca gcctgaagcc tgacgagctc atctcacggc tgggcagttt cgacagggat
      541 gggcaggtct ggcgccatgc ggcccgggaa atttttggcc tggcagcgtt ccgcaccggt
      601 gactacagca tggctgaccg ttacatgaac gccaatttcg ccgatcctga aacgccgcag
      661 gacatgcgcc agcgggcgca agtcatgatc cagctcctta cgccgctgct gcagaagtaa
      721 ttgccatgaa aaaacttccg acatttgtga ttatggccgt ccttgcatca tcgctgatgg
      781 cgtgcagcac cgttaagaga ttcaccggcg aacaggataa cagcgttttg ccgggcaccc
      841 gcgaggaaat tctgacgccc gaccagcaaa cggcgcgtga tccggaagtg acgggcaata
      901 aaaccacgcc gtgcaatccc gacgacattg attgcatcac gccatcgagc gagaccgact
      961 cttctgaaat tgatcaagaa tccagtacgg cgcaatgacg tttacagtcg ctattctcgg
     1021 tcgccccaat gtggggaagt cgacgctatt caaccgcctg gtggggcgca agatcgccct
     1081 ggttgatgac cagccggggg ttacgcggga ccgcagggaa ggcctggcgc aaatcggcga
     1141 cttgagtttc cgcgtcttcg atactgccgg acttgacgac gccaagaagg aaacgcttga
     1201 agcgcggatg agcgcccaag ccgaaacggc ggttcaagac gccgatctcg ttttctttgt
     1261 cattgacgcg cgggcgggga tcacgccgat tgacaggaaa tttgccgaga agatccggaa
     1321 attcggcaag ccggtccgcg tcgtagccaa caaggccgag gcgcgcatgg cggaggccgg
     1381 ggttcttgaa tcctattccc tgggctttgg cgaaccgctt gcgatttcgg ccgagcatgg
     1441 cgaagggatg gagagccttt atgatgtcat cgcaccactg gcacccacga cagaagaagc
     1501 gtccgatgag gaactggaag aagcagaact gacgcggccc ttgcgccttg cgatcatcgg
     1561 ccagcccaat gcgggcaagt ccaccctggt gaataccatg cttggtgaag agcgaatgct
     1621 gacagggccg gaagccggga tcacgcgcga cgccatttcc accgactgga cgttcggggg
     1681 gcgcgagatc aagttgtggg atacggcagg catccggcgc aaatcgcggg tcacaggcaa
     1741 gatcgaaaag ctcgcggtct ccgacgcgct gcgggccatc cgctttgccg actgcgttgt
     1801 cgtgctcatc gacgcttcga tggagatcga gcgccaggac ctggcgcttt ccgaccttgt
     1861 ggcaacagag gggcgggcgg tggttctggc actcagcaaa tgggacctgg tcgaggacaa
     1921 gcagaaggtt ctcgccaagg tccacagcga gcttgaggac gtattgccgg aaatccgcgg
     1981 ggttccggtg gtgacgatat ccgcgaagca ggagcgcggc gttgacaaga tgatgaaggc
     2041 ggtgttcgag gcggttgacc gctggagtgc gcgcatcacc acttcgcagc tcaaccgctg
     2101 gcttgaggct gccatctcga agaacccgcc gcccgcacct tcaggacggc ggatcaagat
     2161 ccgctacgcg acacaggcga gttcacgccc gccgaccttc gcggtattcg gcaaccagat
     2221 ggcaaaaatg ccggaaagct acaagcgcta tctgatgaac acactgcgca aggattttga
     2281 tctggccggt gttcccatcc gcttcagcct gcggggcggc aagaatccct tcgacaagga
     2341 caagtgacga agcagcgtca gagctggacc gatacgtcgc cgatcaggtc atagctggct
     2401 ttaacgaaat caccgatttg ggtttcaaag acgtcggtga tgacgccggt ggtgaccaca
     2461 tcgccctcac gcaggaacaa gccactggca atgagattgt ttgcgagcca ggtgagggcc
     2521 agctccggcc cgcccaaggc attggaggca atgccggtgt atttgcttat gccgttgatg
     2581 gagatgcgat tggtgatccg ggcgtagtct gagcgcgcaa gtggcttgac gcctgggccc
     2641 agcacaaggg cgtggctcag gccaccatcg gccacgtacc attcaaccac atcgttgaag
     2701 cccttgggca aacgcggatt gacgatttcg atggaggggt gaacggtggc cacggcttcg
     2761 agcacttcct caacggacca gggcttatcg cgcgccggca ggtcgcgcgc gagggtgaag
     2821 gcaacttccg gttccgtggt gcggctgttg ctggccaggg tttctacatg ggcaccggct
     2881 ccataaattc gctcccggta gagcggccct ggaaaagggc cgtcggtatg aagggctttc
     2941 tgggcccgct cgctggtgca accgattttc cagccggcga gggtccagcc aatcgtggtg
     3001 cggaccgcag tctggatggc gaatgcttcc gccatgctcg caggcgcagg gccggggtag
     3061 gaggcaagct tctcgcctga caggcgcgcg gcgcgcaagg cgtcgatgat ggaagcctgg
     3121 ctcatagcac ctgcttgaac aggccggtgg gcgcgtattt gtaaaccgcg ccgttgtcag
     3181 tgaaggccgg caggcacatg ggaatgacct gggccgcaac gtcttcggcg gaaggcaggg
     3241 attcggggtc ttcaccgggc atggcctggg cccgcatttt ggtgcgggtg gcgcccgggc
     3301 tgaaacagtt ggcacggatg gcagtctgcc cgttctcggc ggcataggtt ctcaccattg
     3361 cctcaagagc ggctttcgag atggaatagc tcccccaata ggcccagcac ttataggcaa
     3421 ggcccgaggt gacaaagacg gcccggcctg catcagaggc gcgcagcagc ggatcgagcg
     3481 aacggatgag gcggtagttg gcggtgacgt tcacggccat cacatcatcg aaaaccttgg
     3541 gatcgagatg tgcaagcggc gtgagcttgc cgagtatgcc ggcattgccg atcagaatgt
     3601 cgagcttctt ccagcgttcg aaaatggcgg cgcccaagcg gtcgattgcc gcaaagtcct
     3661 tgatgtcgga gggcacaagc gtggccgaac cgccta
//
LOCUS       DAORVN010000132         3671 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_20526_length_3671_cov_3.310288, whole genome shotgun sequence.
ACCESSION   DAORVN010000132 DAORVN010000000
VERSION     DAORVN010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3671
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20526_length_3671_cov_3.310288"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..549)
                     /locus_tag="PKV23_03675"
     CDS             complement(<1..549)
                     /locus_tag="PKV23_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019859775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol/glycerol family dehydratase large
                     subunit"
                     /protein_id="HQX84567.1"
                     /translation="MTETPNRWRRYAEWDARPLRLDKFAAEDAAHGFSAFTSPADPKP
                     GIVIRAGRIASMDGVLAHDFDMIDEFIARHHLDPEIAPEAMAMDSGEVARMLVDMNVP
                     REQLVRLAHGMTPAKLADVVSRLNALEISFAYSKMRARKTPGNQAHVTNAKDDPLQLA
                     ADAATAVAFGFDEIETTMRVSRN"
     gene            703..1587
                     /locus_tag="PKV23_03680"
     CDS             703..1587
                     /locus_tag="PKV23_03680"
                     /EC_number="3.1.1.99"
                     /inference="COORDINATES: protein motif:HMM:NF020039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HQX84568.1"
                     /translation="MAALRDVYQAANKLGEAPIWHAASKRLLWIDLYDPKLFIHDPAK
                     GETLVREISLKAPLGAISATANSKLLIVSHAGGLSTFNIKTGATKIFARPEQDRDAII
                     YNDCKADRFGRLWVGSSHAKETDPRGALWCVLPNGKCFLGDVGFAVSNGPAFSLDGRI
                     MYFNDSVGKKTFAYDISPDDPIPRNRRVLISYTLEEGMPDGLTVDAEGNLWVAHWGGA
                     RITQFTKAGFRLRHIQVPAPHVTSVGFGGENLATLYVTTARDGMSPDALHLWPQSGNL
                     FATKPGVHGIEEPLFPLK"
     gene            1734..3146
                     /locus_tag="PKV23_03685"
     CDS             1734..3146
                     /locus_tag="PKV23_03685"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HQX84569.1"
                     /translation="MARYVDGEVVQRLLKALDFTFFLATTTIYTTSFGDWYDTPNGIS
                     IIAFFALALTFLCLLLLRDLGLYKVSALVNGTWTFAKSAAALVIAGCAAYKGLDYFLI
                     PLGARLSVDWALAVTAYLALSRLLAQAWARPIASQGRFRKRIAIVGGGRVADDALHSL
                     QASRDLEIEVIGLFDDRDVVRSSAPLNAHKKMGTITDLANYARNNRVDLIIVAIPLSA
                     EARLLHILKRLWELPVDIRISGKASDLKFSSKAYTYIGRLPLLSVFDRPLMGWALFLK
                     TMFDRVIASAAIVVLAPVMLAVAAAIRLESTGPILFKQKRYGFNNELIEVFKFRSMYA
                     NRCDANAATLVSKGDPRVTRVGRFIRKTSLDELPQLFNVLTGQLSLVGPRPHATQAKA
                     DNTLYEQVVDGYFARHRVRPGITGWAQVNGWRGETDTREKIEQRVKYDLDYIDHWSLM
                     FDVAILLRTPFALFKNENAY"
     gene            3269..3571
                     /locus_tag="PKV23_03690"
     CDS             3269..3571
                     /locus_tag="PKV23_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019564341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX84570.1"
                     /translation="MDITSLLIQLIIGAVGGNAAGKAVPDKSLGTLGNTIAGAIGGLG
                     GGQLLDMILGGGAMATDAAAASGLDIGAVIQDVAGGGIGGAVLTIVVALIKGAMNK"
BASE COUNT          747 a         1061 c         1075 g          788 t
ORIGIN      
        1 attgcgcgag acgcgcatgg tggtttcaat ttcatcgaaa ccaaaagcca cggcggttgc
       61 cgcgtcggcg gcaagctgca agggatcatc cttggcattg gtcacatggg cctggttgcc
      121 cggtgtcttg cgggcccgca tcttggaata ggcgaaagaa atttcaagcg cattgaggcg
      181 ggagacgaca tcggcgagtt tggccggcgt catgccatgg gccaggcgca cgagttgttc
      241 gcgcggcaca ttcatgtcga ccagcatgcg cgccacctcg ccagagtcca tcgccatggc
      301 ttcgggggca atttccggat cgaggtgatg gcgggcgatg aattcatcga tcatgtcgaa
      361 gtcatgggcc agcacgccgt ccatgctggc gatccggcct gcccttatga ctattccggg
      421 ttttgggtcg gcggggctgg tgaaggcgga gaaaccatga gccgcgtctt ccgccgcaaa
      481 cttgtcaagc cgcaggggcc gcgcatccca ctccgcataa cgccgccacc gatttggagt
      541 ctctgtcaaa cgcccagtcc tgaacctgca aacaggcaga gtgcgggacg agccagcccc
      601 aagtcaagac gcaggccaat tggaacatct gccttggtcc agaatgtggc ctgattcgag
      661 gtttgcggct aaagtggcgg caacggatcc aaggggaaac acatggcagc attgcgggat
      721 gtctatcagg cagcaaacaa actgggcgaa gcgcccatct ggcatgcggc ttcgaagcgg
      781 cttttatgga tcgatcttta cgatccaaag ctctttatcc atgatcccgc caaaggcgag
      841 acgctggttc gcgaaatttc cctgaaggcc ccgctcgggg ccatttctgc cacggccaat
      901 tccaagcttc tgatcgtcag ccacgccggt ggtctttcca ctttcaacat caaaaccggg
      961 gcaaccaaga ttttcgcgcg gccggaacag gaccgtgacg ctataatcta caatgactgc
     1021 aaggcggatc gtttcgggcg gctatgggtc ggcagttcgc acgccaagga aaccgatccg
     1081 cgcggtgccc tgtggtgtgt gctgcccaac ggaaaatgct tccttggcga tgttggattc
     1141 gccgtttcca acggcccggc cttttcgctc gacggaagga tcatgtattt caacgacagc
     1201 gtgggcaaga aaacctttgc ctatgacatc tcgcctgatg acccgattcc gcgcaaccgc
     1261 cgggtgctga tttcctatac gctggaagaa ggcatgcccg acggtttgac ggtggatgcc
     1321 gagggcaacc tttgggtggc tcattggggg ggcgcccgga taacgcagtt cacgaaagcc
     1381 ggtttccggc ttcgccatat tcaggtgcct gccccgcatg tcacatcggt gggcttcggc
     1441 ggcgagaacc tcgccaccct ttatgtaacg acggcacggg acggtatgag cccggacgcc
     1501 ctgcacctgt ggccgcagtc cggcaacctg tttgcgacca agccgggggt tcacgggatt
     1561 gaagagccac tttttccgct gaagtaacca ttcatcaagg ttaatggcat cctaatccgg
     1621 gccaaatcgc cagcattttc actaattttt agccattggc tttaaatttg cattgcaggg
     1681 aggcaagcgc aatcaagtgc agagttggga caatggcggc ggcaacaagg acagtggcaa
     1741 gatatgttga cggcgaagtc gttcaacgcc tgctgaaggc tctggatttt accttcttcc
     1801 ttgccactac cacgatctac acgacgagtt tcggagactg gtacgacacc ccaaacggca
     1861 tctcaatcat tgcctttttc gccctggccc tgacgtttct ctgcctgctg ctgctacgcg
     1921 atctcgggct ttacaaggtg tcggccctgg tcaacggcac ctggaccttt gcgaaatcgg
     1981 cggcggctct cgtcattgcc ggttgtgccg cctacaaggg gctcgactac tttctcattc
     2041 ccctgggggc gcgcctgtcg gtcgactggg cgctggccgt caccgcctat ctggccctca
     2101 gccgcctgct ggcacaggcc tgggcccggc ccattgcgag ccagggacgg tttcgcaagc
     2161 gcatcgccat tgtgggcggc ggacgggtcg ccgacgatgc cctgcattcc ctgcaggctt
     2221 cacgcgacct ggagattgaa gtcatcggcc tgtttgacga ccgcgatgtg gttcgcagct
     2281 cagcgccgct taatgcccac aagaagatgg gaacaatcac cgaccttgca aactatgccc
     2341 gaaacaaccg ggtcgatctg atcatcgtgg ccattccgct ttcggcggaa gcgcgccttc
     2401 tccacatcct gaaacggctg tgggaattgc ccgtcgatat ccgaatcagt ggtaaagcct
     2461 cggatctcaa attcagttcc aaggcctata cctacatcgg ccgcctgccg ctgctttcgg
     2521 tattcgaccg gccgctgatg ggctgggcac tgttcctcaa aacaatgttt gaccgcgtca
     2581 tcgcaagcgc tgccattgtc gtgctggcgc cggtgatgct tgctgtggcc gcggcaatcc
     2641 ggctggaaag cacggggccg attctgttca agcagaagcg ctacggcttc aacaatgaac
     2701 tgatcgaagt tttcaaattc cgctccatgt atgcgaaccg ctgcgacgcc aacgcagcaa
     2761 cactggtgag caagggcgac ccccgtgtca cgcgtgtggg gcgcttcatc cgcaaaactt
     2821 ctctggacga attgccgcaa ttattcaacg ttttaaccgg ccagctgtca cttgtgggcc
     2881 cacgcccgca tgccacccag gccaaggcgg acaacacgct ttacgagcag gtcgtcgatg
     2941 gctatttcgc ccgccaccgg gtgcgtcccg gaatcaccgg ctgggcgcaa gtgaacggct
     3001 ggcgcggcga gaccgatacg cgcgagaaaa tcgaacagcg ggtgaaatat gacctggatt
     3061 acatcgacca ctggtccctg atgtttgacg tggcgatcct gttgcgcacg cctttcgcgc
     3121 ttttcaaaaa tgaaaacgcc tactgaagat tcttccgatc ttcgccacag gccgcggcgc
     3181 ggttatcaga ctttgagttg acgttcgaaa tctgttatat ttgcgcactg gcagcaagcc
     3241 agtgatcgtc cacaaaaggg gaaccaaaat ggacattact tcgcttctta tccaactcat
     3301 catcggcgcc gtcggcggca acgcggcggg caaggcagtg cctgacaaga gtctcggtac
     3361 tctgggtaac acaattgcgg gtgccattgg tggattgggc ggcggccaac ttcttgatat
     3421 gatacttggt ggcggcgcga tggcgactga tgcggcagcg gcaagcggcc tggacattgg
     3481 cgcggtcatc caggatgtgg cgggcggcgg catcggcggt gccgtgctga cgattgtggt
     3541 ggccctcatc aagggcgcga tgaacaagta agtttcgttg gaatttcgaa cgggcgcaga
     3601 ttaactgcgc ccgtttttta tttgcaccat gttcgcgtta tcagagctgg tcagttccaa
     3661 aagtatcgca c
//
LOCUS       DAORVN010000133         3669 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_20545_length_3669_cov_4.048146, whole genome shotgun sequence.
ACCESSION   DAORVN010000133 DAORVN010000000
VERSION     DAORVN010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3669)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3669)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3669
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20545_length_3669_cov_4.048146"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..985)
                     /locus_tag="PKV23_03695"
     CDS             complement(<1..985)
                     /locus_tag="PKV23_03695"
                     /inference="COORDINATES: protein motif:HMM:NF016274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HQX84571.1"
                     /translation="MATLKEQGFYMPGEWHPHRRCWMAWPSNAGGYSGQFAEARQAAA
                     RVAAAIACFEPVIMLANETDIIEAQTLCGLSVEVQAAPIDDGWFRDNGPSFVIDGRGN
                     IRGVNWDFNGWGRKPGIPFTRDRAVTKHILAREEIACVASPLILEGGSFHVDGEGTLL
                     TTRQCLLNPNRNPAWGQASIERELKDHLGADTVIWLNRGVVDDFTDGHIDLLAAFVKP
                     GVVLALGCDDAADANYTALQENLEIFRGARDAKGRSFEVIEVPQPPAAYNEATGTRLG
                     LSHLNFYLANGGLILPDFGNPESDAAAQGLFRELFKDLEIVPVPSRALLYTD"
     gene            complement(1035..1595)
                     /locus_tag="PKV23_03700"
     CDS             complement(1035..1595)
                     /locus_tag="PKV23_03700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX84572.1"
                     /translation="MTRTTCVLAMIWMLAGISAEAKDRLSDLPGPVAAYAEQFSKDCQ
                     ARGLGEAVVNENYRAGNPGPADVNGDGAPDYVIYKCMFGCSEKPFAFSGVGTPCPFGK
                     LLLSQAGQYAEVFLPGLISRIEAGPPVWISLQRPSSLRTAGNFCRDPFPDSDPVFVYE
                     LKEKRFQVVGSCPSDGACELASAAGL"
     gene            complement(1688..2521)
                     /locus_tag="PKV23_03705"
     CDS             complement(1688..2521)
                     /locus_tag="PKV23_03705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX84573.1"
                     /translation="MNQLPGKKERNAFNRASPAALAEGNEQELGELSLSGTHEQAVPF
                     LRAASQLGGKVVQFAVQPVIDYNPRKISKKVLAAMSGDPDPVKTLSDIRSLLVGPTRS
                     LHDAMFEEVIDILEESDREVQTALETLESNYYRLSDITDGLVTASVESRDKTRQQTEF
                     FQAELQKSVAGQQEMLSEMFMAIDAKIAEMTARVDRKIEALAVKLEASQREAEIAQER
                     NNQALEARCLASAAEAIDMLEARLGHLERKSSTDQAVIREVFADGLLNIADRFKTLHH
                     G"
     gene            complement(2663..3316)
                     /locus_tag="PKV23_03710"
     CDS             complement(2663..3316)
                     /locus_tag="PKV23_03710"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007606460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HQX84574.1"
                     /translation="MCNLYSITSSQSYVRELARALRDISGNQPPLPGVFPDYVAPIAR
                     NNGGTRELATARWGMPTPLFALKGRNSDGGITNVRNVKSPHWRRWLGVESRCLVPFNS
                     FSENEVLADGSRPPVWFASSEERPLAFFAGIWTPQWKSVRKVKEGETTNDLFAFLTTE
                     PNREVGAVHPKAMPVILTTPAEIEIWMTAPAEEALKLQRPLPDGALQIVARGQKKDP"
     gene            complement(3357..>3669)
                     /locus_tag="PKV23_03715"
     CDS             complement(3357..>3669)
                     /locus_tag="PKV23_03715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX84575.1"
                     /translation="YCGATSPPWGLRWDDRSVRMSLFPGKHEFEIQSVKPGVSPGSAL
                     VTASAAEAAVSFVYSDEICHGTDNATVSWGSAYIVIREGGVERLYKGCCNPLRIAFTN
                     R"
BASE COUNT          657 a         1099 c         1203 g          710 t
ORIGIN      
        1 cgtcggtgta gagcagggcg cgggagggga cggggacgat ctccagatct ttgaacagct
       61 cgcggaaaag gccctgcgct gccgcatcgg attcagggtt tccgaagtcg ggcaatatga
      121 gtccgccgtt ggcgaggtag aagttgaggt gcgagaggcc aagcctggtg ccggttgcct
      181 cgttgtacgc ggcgggcggc tggggcacct cgattacttc gaagctgcgg cccttggcgt
      241 cgcgggcgcc gcggaagatt tccaaattct cctggagggc ggtgtagttg gcgtcggccg
      301 cgtcatcaca gcccagcgcc agcaccacgc cgggcttcac gaaggccgca agcaggtcga
      361 tatggccgtc ggtgaaatca tcgaccacgc cgcggttcag ccagatcact gtgtcggcgc
      421 cgagatggtc tttcagttcg cgctcgatcg aagcttgccc ccaggccggg ttgcggttgg
      481 ggttcagcag gcattgcctt gtggtgagca atgtgccctc accatccaca tggaatgagc
      541 cgccctcgag gatgaggggc gaggccacgc aagcgatttc ctcgcgcgca agaatgtgtt
      601 tggtgacggc gcggtcgcgg gtgaagggaa tgccgggttt gcggccccag ccgttgaaat
      661 cccagttcac acccctgatg ttgccgcggc cgtcgatcac gaagctcggg ccattgtcgc
      721 ggaaccagcc gtcatcaatg ggcgcggcct gcacctcgac cgaaagaccg cacaaagtct
      781 gagcctcgat tatgtcggtc tcattggcca gcatgattac cggttcgaag caggcgatgg
      841 ctgcggccac gcgggctgcc gcctggcggg cctcggcaaa ctggccgctg taaccgcccg
      901 cattgctcgg ccaggccatc cagcagcgcc tgtgcggatg ccattcgccc ggcatataga
      961 atccctgttc cttcagcgtg gccatgggtt cacgctaagg tgagcggggc ggggatgcaa
     1021 acggcttctg cccgttagag tccggcggcg ctggccaact cacaggcgcc gtcggaaggg
     1081 caactgccca ccacctggaa gcgcttctcc ttcaactcgt agacaaacac cggatcggaa
     1141 tccggaaagg gatcccggca aaaatttccg gcggtgcgaa gcgaactcgg gcgctgcagg
     1201 gaaatccaga cgggcgggcc ggcctcgata cggctgatca ggccgggcag gaaaacttcc
     1261 gcgtattggc cggcctgcga cagaagcagt ttgccaaagg ggcagggggt tccgacgccc
     1321 gaaaaggcaa agggcttttc gctgcagccg aacatgcact tgtagatgac gtaatcgggg
     1381 gcgccatcgc cgttcacatc ggcgggaccc ggattgccgg cccggtaatt ctcgttgacg
     1441 acggcctcgc caaggccgcg cgcctgacaa tctttgctga attgttccgc ataggcggcc
     1501 acggggccgg gcagatcgct gagcctgtct tttgcctctg cggaaattcc cgccagcatc
     1561 cagatcattg caagcacgca ggtcgttcgg gtcatgggag catctcactc cggaacggca
     1621 gccaaggctg catgatagac caatccggca acgccttcaa cgtgccggac tggttctcaa
     1681 tgcaagctca accgtggtgc agggttttga agcggtcggc gatattcagc aggccgtcgg
     1741 cgaagacctc gcggatgact gcctgatcgg tgctcgactt cctttcgaga tggccgagac
     1801 gcgcttcgag catatcgatg gcctcggcgg cgctggccag gcaacgcgct tccagggcct
     1861 ggttgttgcg ttcctgggcg atttcggcct cgcgttggga agcttcgagc ttcacggcca
     1921 gggcctcgat cttgcggtcc accctggcgg tcatctcggc gatcttggcg tcgatggcca
     1981 tgaacatttc ggagagcatt tcctgctggc cggccaccga cttctgcagt tccgcctgga
     2041 agaattcggt ctgctggcgg gtcttgtcgc ggctctcgac ggaggcggtc accagtccgt
     2101 cggtgatgtc ggagagacgg taatagttgc tctccagcgt ctcgagcgcg gtctggactt
     2161 cgcggtcgga ttcctcgagg atgtcgatca cttcctcgaa catcgcatca tgcaggctgc
     2221 gcgtcgggcc cacgagaagc gaacggatgt cggacagcgt cttcaccgga tccggatcgc
     2281 cggacatggc ggccagaacc ttcttgctga ttttgcgcgg attgtaatcg atcaccggct
     2341 gaaccgcgaa ctgcaccacc ttgccgccga gctggctggc ggcgcgcagg aagggcacgg
     2401 cctgttcatg ggtgccggac aggctcagtt cgccaagctc ctgctcgttg ccctcggcga
     2461 gggctgcagg cgatgcccgg ttaaaggcgt tgcgctcttt cttgcccggt aattgattca
     2521 tccctcacac tcccactcac tgttacactt accccgcgtc caccggaatt ttgcaatagt
     2581 tacagcgatt taagcccgca cccgtgaaca gatcgaatcg gacacagata tgtggccatt
     2641 gtgtggcatg gttaatggcg gctcaggggt ctttcttctg cccacgggcc acgatctgca
     2701 gggcgccgtc gggcaggggc cgctgcagtt tcaacgcttc ctcggcgggc gccgtcatcc
     2761 agatttcaat ttccgccggc gtcgtaagga tcaccggcat ggccttgggg tgaacggcgc
     2821 ccacttcgcg gttgggttcg gtggtgagaa aggcgaagag gtcgtttgtg gtttcgccct
     2881 ccttcacctt gcgcacggat ttccattgcg gcgtccagat gccggcgaag aaggcgaggg
     2941 ggcgctcttc gctcgaggca aaccacacgg gcgggcgcga gccatcggcc agcacttcgt
     3001 tttccgaaaa gctgttgaac ggcacaaggc agcggctttc cacgccgagc cagcgccgcc
     3061 agtggggcga tttcacattg cggacattgg tgatgccgcc gtcggaattg cgacccttca
     3121 gggcgaagag gggtgtgggc atgccccagc gcgcggtcgc cagttcgcgg gtgccgccgt
     3181 tgttgcgcgc gatgggggca acataatcgg ggaagacgcc ggggagcggc ggctggtttc
     3241 ctgaaatgtc gcgcaaggca cgcgccagtt cgcgcacata gctctggctt gaagttatcg
     3301 aatagagatt gcacatgggg ggcgacttta gcccggcgat tccccggcga acaggtctag
     3361 cggttggtga aagcaatccg caggggattg cagcagccct tgtagagccg ttccacgccg
     3421 ccctcacgaa tgacgatata ggcggagccc cagcttacgg ttgcgttgtc cgtcccatgg
     3481 cagatttcat cgctgtagac aaaggacacc gccgcttcgg cagcggacgc ggtcaccagc
     3541 gcggagcccg gcgagacgcc cggcttcacc gattggattt caaattcgtg ctttcccggg
     3601 aacagcgaca tgcgcacgga tcggtcgtcc cagcgcaggc cccagggcgg cgaggtggcg
     3661 ccgcaatag
//
LOCUS       DAORVN010000134         3668 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_20562_length_3668_cov_3.047606, whole genome shotgun sequence.
ACCESSION   DAORVN010000134 DAORVN010000000
VERSION     DAORVN010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3668
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20562_length_3668_cov_3.047606"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            11..154
                     /locus_tag="PKV23_03720"
     CDS             11..154
                     /locus_tag="PKV23_03720"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="laccase domain-containing protein"
                     /protein_id="HQX84576.1"
                     /translation="MKSFGTGAVIDSGLCTYKREAEYFSYRRATHAREKDYGRQIAAI
                     TLT"
     gene            163..1314
                     /locus_tag="PKV23_03725"
     CDS             163..1314
                     /locus_tag="PKV23_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004689854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HQX84577.1"
                     /translation="MALHFEPSEYRQRIAAAIAAMQKANLDGLLMFQQESMYYLTGYD
                     TFGFCFFQCLYLGADGKLALLTRSADLRQAQHTSNIQDIRIWTDQAGAQPATQLRDML
                     ESLGARGKRLGIETNSYGLTHFNGKAVDAAMEGFCTLVESTNLVDRLRLVKSPAELKY
                     VRKAGKLGDEALKAGIKKAKAGADEGDILAAQHSAIFSGGGDYPGNEFIIGSDRDALL
                     CRYKSGRRKLSKRDQLTLEFAGVYRHYHAAFMRTLIIGKPNKDHLKMDEACRAALSEV
                     EGVLRPGKTAGEVFDAHARVMDAHGMREHRLNACGYSLGAKFTPSWMDFPMFYRGNEV
                     VIAEGMVFFAHMILMNSASEAAYCLGRTYIIGTRKPEPVSKYPLTMIAR"
     gene            1311..1922
                     /locus_tag="PKV23_03730"
     CDS             1311..1922
                     /locus_tag="PKV23_03730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX84578.1"
                     /translation="MTRLLSILFLLMLAACAAGGSQPFGKLEPAERTSAIISPPWEAM
                     IAAGPGASKEIDLETLNGPGRQQAAAATPEDIAADAKSGRLVIRAVAVIPVKGAKGTG
                     NDELTRAMRQTLTLAGWTVLSAPAKNALTISGRVEMADVSGNMQQVALNWAVQAPDGG
                     KLGDVNQANKVPAGSLDAGWGDIAGAAVEAAASGIFELINKFR"
     gene            2139..3071
                     /locus_tag="PKV23_03735"
     CDS             2139..3071
                     /locus_tag="PKV23_03735"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HQX84579.1"
                     /translation="MKIVAGNSNRPLAEAICSYLHLPQVKAVVKRFNDMEVFVEIQEN
                     VRGQDMFVVQSTSFPANDHLMELLIIIDALKRASAKRVTAVLPYFGYARQDRKPGPRT
                     PISAKLVANLITRAGADRVLTLDLHAGQIQGFFDIPTDNLFAGPVLVRDIKQHNDVAN
                     SVVVSPDIGGVVRARALAKRLDTPLAIVDKRREKAGESEVMNIIGEVAGKSCILIDDI
                     VDSGGTLCNAAEALLAQGAKDVSAYITHGVLSGGAVARITSSKLKHLVITDSIQPTEA
                     VKVARNIRVIGIASLLGEAIGRTSREESVSSLFE"
     gene            3361..3537
                     /locus_tag="PKV23_03740"
     CDS             3361..3537
                     /locus_tag="PKV23_03740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX84580.1"
                     /translation="MDCQDCCLGLSCVSSGVVLQDAMGMTKRLPSGQPQAISDDHLYG
                     RSIAPETGPPKLPA"
BASE COUNT          740 a         1101 c         1124 g          703 t
ORIGIN      
        1 gatggcgcgc atgaaaagct tcggcaccgg cgcggtcatc gacagcgggc tttgcacgta
       61 taagcgcgag gcggaatatt tctcctatcg ccgcgccacg catgcacggg agaaggatta
      121 cggacggcag atcgccgcca tcacgttgac ctaggggcat tcatggcact tcatttcgag
      181 ccttccgaat accggcagcg catcgccgcg gcgattgccg ccatgcagaa agccaatctc
      241 gacggcctgc tcatgttcca gcaggagagc atgtattatc tcacgggcta cgacaccttc
      301 ggcttctgct ttttccagtg cctctatctc ggcgccgatg gcaagctggc gctgctcacc
      361 cgctcggcgg atttgcgcca ggcgcagcat acctccaaca ttcaggacat ccgcatctgg
      421 accgatcaag ctggagccca gcctgcaacc cagttgcggg atatgctgga aagcctcgga
      481 gcgcgcggca agcgtcttgg catcgaaacc aattcctatg gcctcacgca tttcaacggc
      541 aaggcggtgg atgccgcgat ggagggtttt tgcactctgg ttgaatcgac caatcttgta
      601 gaccggctgc gcctggtcaa atcgccagcc gagctcaaat atgtgcgcaa ggccggcaag
      661 cttggtgatg aagccctgaa ggccggaatt aaaaaagcaa aggcgggcgc cgatgagggc
      721 gatattctcg ccgcccagca ttcagccatt ttctccggtg gcggagacta cccgggcaat
      781 gaattcatca tcggatcgga ccgtgacgcg ctgctctgcc gctacaagtc gggccgccgg
      841 aaactgtcga aacgcgacca gctcacgctg gagttcgcgg gcgtctatcg ccactaccat
      901 gcggccttca tgcgcacact gatcatcggc aaaccgaaca aggatcatct gaagatggat
      961 gaggcctgcc gcgcggcact cagcgaagtt gaaggcgtat tgcggccggg gaaaaccgct
     1021 ggcgaggtgt ttgacgctca cgcccgggtg atggatgccc atggcatgcg cgagcaccgg
     1081 ctcaatgcct gcggctattc gctgggggca aagttcaccc cgagctggat ggatttcccc
     1141 atgttctatc gcggcaatga agtcgtgatc gcggaaggca tggtgttctt tgcccacatg
     1201 atcctgatga attccgcatc ggaggctgcc tattgcctgg ggcgcaccta tatcatcggg
     1261 accaggaagc ccgaacccgt ttcgaaatat cctctcacca tgatcgcaag atgacacgac
     1321 tgctgtccat tctcttcctg cttatgcttg cggcttgtgc cgcaggaggc agccagccct
     1381 tcggcaagct tgagccggcg gagcggacgt cggccatcat ttccccgccc tgggaggcga
     1441 tgatcgcggc agggccggga gcgtcgaagg agatcgatct ggaaaccctc aatggcccgg
     1501 gcaggcagca ggcggctgcc gcgacgccgg aggatattgc cgctgacgcc aagagcgggc
     1561 ggctggtcat cagggccgtg gcggtcattc cggtcaaggg ggccaagggc acgggcaatg
     1621 acgaattgac ccgcgccatg cgccagacct tgaccctggc gggctggacg gtgctcagcg
     1681 ccccggccaa gaatgcgctc accatttcag gccgggtcga gatggcggac gtttcgggca
     1741 acatgcagca ggtggcgctc aactgggcgg tgcaggcacc cgatggcggc aagctcggcg
     1801 atgtgaacca ggccaacaaa gttcccgccg gctcgctcga cgcgggctgg ggcgatatcg
     1861 ccggcgctgc ggtggaagcc gccgccagcg ggatcttcga actgatcaac aagttcaggt
     1921 gatgcctgac ctcaccctga ggtgccttgc gtgagcaagg cctcgaaggg ccggctgcct
     1981 cggaaacaga tgcttcgagg ctcacgctgg cgcgtgagca cctcagcatg aggggattga
     2041 agggggttct taacactccc cacaggcggc atttgcgctt gtgtcacacc cttgttatag
     2101 agcgcccaca ggcagcgcgg cgacgaggga caagcatgat gaaaatagtg gcgggtaaca
     2161 gcaaccggcc cctggcggaa gccatctgtt cctacctcca cctgccgcag gtcaaggccg
     2221 tggtcaaacg cttcaacgac atggaagtgt tcgttgaaat ccaggaaaac gtccgcggcc
     2281 aggacatgtt cgtggtgcag tccaccagct tcccggccaa cgaccatctg atggaactgc
     2341 tgatcatcat cgatgccctg aagcgggctt cggccaaacg cgtgaccgcg gtgctgcctt
     2401 atttcggcta tgcccgccag gaccgcaaac ccggaccgcg cactcccatt tcagccaagc
     2461 ttgtggccaa cctgattacc cgcgccgggg ccgaccgggt gctcacgctc gatcttcatg
     2521 cggggcaaat ccagggcttc ttcgacatcc ccaccgacaa tctcttcgcc ggaccggtgc
     2581 tggtgcgcga catcaagcag cacaatgatg tggcaaattc ggtcgtggtc tcgccggaca
     2641 tcggcggcgt ggtgcgcgcc agggcccttg ccaagcggct cgatacgccg ctcgccatcg
     2701 tcgacaagcg ccgcgagaag gcaggcgagt cggaagtcat gaacatcatc ggcgaagtgg
     2761 cgggcaagtc ctgcatcctg atcgacgaca tcgtcgattc cggcggaacg ctgtgcaacg
     2821 ccgccgaggc gctgctggcg cagggcgcca aggatgtgag cgcctatatc acccacggag
     2881 tgctgtcggg cggtgcggtg gcgcggatca cctcgtcaaa gctcaagcat ctggtgatca
     2941 ccgattcgat ccagcccacg gaagcggtga aggtggcgcg caatatccgg gtcatcggca
     3001 tcgccagcct gctcggcgaa gcgatcggcc gcacgtcgcg cgaagagtcg gtgtcgagcc
     3061 tgttcgagta ggcgacatga accggggttc aacatgtgcg ctgtcatcta tcgagggatt
     3121 gttttaaatc aacaaagata agttgaagtg gtctatagag tgtcgatgcc cggttatcgg
     3181 ttcatagttt tggctgccgc catgttaatg atggtcgtat ccatcggttt gggcgcatct
     3241 gccggtatgg ccaactgcgc accacaacag cccttttccg tcagtgacca tgctggtcat
     3301 ggcacggatg caagcgccgg ctgtcaagct tctgattgtc aggacgtgga ccatcgcgat
     3361 atggactgcc aggattgctg tcttggcctg tcctgtgtct cgtccggcgt tgttttgcag
     3421 gacgccatgg gcatgaccaa gcgcctgccg tccggccagc cccaggcaat ttcggacgat
     3481 catctgtacg gccggtccat tgccccggaa acaggccctc caaagctccc cgcctgagtg
     3541 atcccggacc ggtcggtccg gagcagccca cgtgttcaaa acccaagatc atgtttcagg
     3601 tgccgattgc atgtcggcaa ttttgaagga gctataaatg gcatatcaca atctatccaa
     3661 ttcaatcg
//
LOCUS       DAORVN010000135         3652 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_20708_length_3652_cov_3.555741, whole genome shotgun sequence.
ACCESSION   DAORVN010000135 DAORVN010000000
VERSION     DAORVN010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3652
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20708_length_3652_cov_3.555741"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..989
                     /locus_tag="PKV23_03745"
     CDS             <1..989
                     /locus_tag="PKV23_03745"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HQX84581.1"
                     /translation="ASGVMTTLVLIFSEVMPKTYAIAYPERVALAAAPIIRVLVAVFG
                     PVVMTVEYIVKSVLRLFGVDITNAQNVLSAHDELRGTIDLHHKEGAFITNDRNMMGGI
                     LDLQDLEVLDAMIHRTKMLTIDSADAPEKIVNQVLKSGHTRFPVWKDTQDNIVGILHA
                     KNLFAALQESEGDFSKVRIEEVMSPPWFVPDTRPLADQLKAFLRRKTHFAVVVDEYGE
                     VQGLITLEDIIEEIIGDIKDEHDFVSSGIKPQPDGSYIVEGTVPLRDLNRALGWNLPD
                     DEATTIAGLVIHDARMIPDVGQVFNFHGFRVEVLKKRKHQVTSVKLTPENPG"
     gene            complement(986..1261)
                     /locus_tag="PKV23_03750"
     CDS             complement(986..1261)
                     /locus_tag="PKV23_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014490401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HQX84582.1"
                     /translation="MNLGLVGKTLETKLQLAFSPVSLQVIDESLQHVGHAGARPDGES
                     HFRVKIVAAAFQGKSRVAQHRMINHVLAAELKERVHALAIEAAAPED"
     gene            1322..1918
                     /locus_tag="PKV23_03755"
     CDS             1322..1918
                     /locus_tag="PKV23_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018183429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HQX84583.1"
                     /translation="MKLDSKYFDKIRIKTGPNKKEPPAPAMCEWPGCAKPSRHKAPKG
                     RGAEGQFHNYCTQHVQEYNKTYNYFEGMKDDAVAGFQKDAQTGHRPTWKLGQNSYANI
                     NGMRRKARNVNDPFAMFTPSAENVNAKLGRALRKNEIKALNTLGLDETATSEKAKSQY
                     KLLVKRLHPDANGGSRENEDTLKAVIHAYDTLRASGFC"
     gene            2018..2998
                     /gene="cobS"
                     /locus_tag="PKV23_03760"
     CDS             2018..2998
                     /gene="cobS"
                     /locus_tag="PKV23_03760"
                     /EC_number="6.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013417996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051116 - cobaltochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobS"
                     /protein_id="HQX84584.1"
                     /translation="MANGDAHGMPDSTASVSQLFGFESNMIVPSYSTATEHVPDLDED
                     YLFNRDTTLAILAGFAFNRRVMITGYHGTGKSTHIEQVAARLNWPCIRINLDSHISRI
                     DLIGKDAIVLQDGKQVTEFREGILPWAYQRNVALVFDEYDAGRPDVMFVIQRILESSG
                     KLTLLDQSKVIRPHKAFRLFSTANTVGLGDTTGLYHGTQQINQAQMDRWSIVSALNYL
                     PHAEEVGIVLAKCKNYDTEKGRKIIANMVRIADLTRNAFMQGDVSTVMSPRTVITWAQ
                     NADIFGDLGFAFRLTFLNKCDELERPIVAEFYQRCFGEELPESSAHVAIA"
     gene            3013..>3652
                     /locus_tag="PKV23_03765"
     CDS             3013..>3652
                     /locus_tag="PKV23_03765"
                     /inference="COORDINATES: protein motif:HMM:NF017973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobT"
                     /protein_id="HQX84585.1"
                     /translation="MAEAREGKAEEFKRVLSTAMKTIADDPELTVSFGNEAPALSGNK
                     CKLPQVTSQFTPKDIAITRGLSDSFALRLSNHSDQIHSKYRPEGKNARAVFEAVEQAR
                     IEALGANAMPGMAQNLNAALEDRFAKAVVNRSSNRRDTPLEEAVALMVRERLTGQAPP
                     ANAKMYVDLWRPWIEEKAASRLSNLGSAIHDQTEFARASRDIIAALDMAADLD"
BASE COUNT          803 a         1164 c          986 g          699 t
ORIGIN      
        1 tcgcatccgg cgtgatgacc actctggtgc tgatcttcag cgaagtcatg cccaagacct
       61 atgccatcgc ctatcccgaa cgcgtggcgc tggcggccgc acccatcatc cgggtgctgg
      121 tggccgtctt cgggccggtc gtgatgaccg tcgaatacat cgtgaagtcg gtgttgcgcc
      181 tgtttggcgt cgacatcacc aatgcccaga acgtgctgtc cgcccatgac gaattgcgcg
      241 gcaccattga tctgcaccac aaggaaggtg ctttcatcac caatgaccgc aatatgatgg
      301 gcggcattct cgatcttcag gatctcgagg tgctggatgc catgatccac cgcaccaaga
      361 tgctgaccat cgactctgcc gacgcgcccg agaagatcgt caaccaggtc ctgaaatcag
      421 ggcatacgcg ctttcccgtg tggaaggata cgcaggacaa catcgtaggc atcctgcacg
      481 ccaagaatct cttcgctgct ttgcaggaga gtgaaggcga cttcagcaag gtcaggatcg
      541 aggaggtcat gagcccgccg tggttcgtgc ccgacacgcg gcccctggct gaccagctca
      601 aggccttcct ccggcgcaag acccactttg ccgtggttgt cgacgaatat ggtgaagtcc
      661 agggcctcat taccctggag gacatcattg aagagattat cggcgacatc aaggacgagc
      721 atgatttcgt aagctccggc atcaagccgc agcccgacgg ttcatacatc gtcgagggca
      781 ccgtgccctt gcgtgacctc aaccgcgccc tgggctggaa cttgccggac gatgaagcta
      841 ccaccatagc cggcctcgtc atccatgatg cccgcatgat cccggacgtg ggccaggtct
      901 tcaattttca cggtttccgc gtggaagtgc tcaagaaacg caaacaccag gtaacttcgg
      961 tcaaactgac tccggagaat cctggctagt cttctggggc tgccgcctcg atcgccagcg
     1021 catgcacccg ctctttgagt tctgccgcca ggacgtggtt aatcatccgg tgctgcgcga
     1081 cccgggactt gccctgaaac gccgccgcga caattttgac gcggaaatgg ctttccccgt
     1141 ctggccgggc gccggcatgg cccacatgct gcagcgattc atcaataacc tgcagggaaa
     1201 cgggtgaaaa tgccaactgc agcttcgttt caagggtctt tccgaccagt cctagattca
     1261 tgctgtgtcc agtgcggttc agggttgcga cttttgcttc atatccccat aatcatgccc
     1321 catgaaactt gattcaaaat atttcgacaa gattcgcatc aaaaccggcc ccaacaagaa
     1381 ggagccgccg gccccagcca tgtgcgaatg gccgggttgc gccaagccgt ctcggcacaa
     1441 ggcgcccaag gggcgtggcg ccgaaggcca gtttcacaac tactgcaccc agcatgtcca
     1501 ggaatacaac aagacctaca attattttga ggggatgaag gatgacgcgg tagccggctt
     1561 ccagaaggac gcccagaccg gccatcggcc gacctggaaa ctgggccaga actcctatgc
     1621 caatatcaac ggcatgcgcc gcaaggcccg caatgtgaac gatccttttg ccatgttcac
     1681 gcccagcgcc gagaatgtta atgccaagct gggccgcgcc ctgcgcaaga acgaaatcaa
     1741 ggcgctcaac accctgggac tggacgaaac ggccacctct gaaaaggcca agtcgcagta
     1801 caagctcctg gtgaaacgcc ttcaccccga cgccaatggc ggctcacgcg agaatgagga
     1861 caccctgaaa gcggtgatcc atgcttacga cacattgcgg gccagcggtt tctgctagtt
     1921 tacccgtttt ctggaacttg cagcccccct ttgaaccgtt tagaacccaa agcattccac
     1981 gacacgcccg gaagagaaga atgacaagat gaacattatg gcaaatggtg acgcccacgg
     2041 catgcccgat tccaccgcat cggtaagcca gcttttcggc ttcgaatcga acatgatcgt
     2101 gccgtcctac tcgacggcaa ctgagcacgt gcccgatctg gatgaggact atcttttcaa
     2161 tcgcgacacc acgctggcaa tcctggcggg ctttgccttc aaccgccgcg tgatgatcac
     2221 cggctaccac ggcaccggca agtcgaccca tatcgagcag gtcgccgccc gcctcaactg
     2281 gccctgcatc cgcatcaacc tcgacagcca catcagccgc atcgatctca tcggcaagga
     2341 cgccattgtg ctgcaggatg gcaagcaggt caccgaattc cgcgaaggca ttttgccctg
     2401 ggcttatcag cgcaacgtgg cgctggtctt cgacgaatat gacgcgggcc gtcccgacgt
     2461 gatgtttgtc atccagcgca ttctcgaatc ctccggcaag ctcaccctgc ttgaccagtc
     2521 gaaggtcatc cgcccgcaca aggccttccg cctcttttca acggccaaca cagtgggcct
     2581 cggcgacacc accggccttt atcatggcac ccagcagata aaccaggccc agatggaccg
     2641 ctggtccatc gtctccgctc tgaactatct gccccatgcc gaggaagtcg gcatcgtgct
     2701 ggccaagtgc aagaattacg acaccgaaaa gggccgtaag atcattgcga acatggtacg
     2761 catcgccgat ctcacccgca acgccttcat gcagggcgat gtctcgaccg tgatgagccc
     2821 gcgcaccgtc atcacctggg cgcagaacgc cgacatcttt ggtgatcttg gttttgcctt
     2881 ccggctcacc ttcttgaaca aatgcgacga gcttgaacgc ccgatcgtgg ccgagttcta
     2941 ccagcgctgc tttggcgagg aattgcccga gtcatcggcc catgtggcca ttgcctgaaa
     3001 gcccatcagc ccatggccga ggcccgtgaa ggcaaagcag aggaattcaa gcgcgttctg
     3061 agcacggcga tgaaaaccat tgccgatgat ccggaactca ccgtgagttt cggcaatgaa
     3121 gcgccggctt tgtccggcaa caagtgcaag cttccccagg tcaccagcca gttcaccccc
     3181 aaggacattg ccatcacccg cggcctgtcc gacagttttg ccctccggct ctccaaccac
     3241 tcggatcaga tccacagcaa gtaccgcccc gaaggcaaga atgcccgcgc tgtgtttgaa
     3301 gccgttgaac aggcccgcat cgaggcgctg ggcgccaatg ccatgccggg catggcgcaa
     3361 aacctgaatg ccgccctgga agaccgcttt gcaaaagccg tggtcaaccg ctccagcaac
     3421 cgccgggata cccctctcga agaggccgtg gcgcttatgg tgcgtgagcg cctcacgggc
     3481 caggcgccgc ctgccaacgc caagatgtat gtcgatctct ggcgtccatg gatcgaggaa
     3541 aaggcggcat ctcgcctcag caatctgggc agcgccattc acgaccagac ggagtttgcc
     3601 cgggcctcgc gcgatataat cgccgccctc gacatggccg ccgatctcga tg
//
LOCUS       DAORVN010000136         3617 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_21073_length_3617_cov_3.503930, whole genome shotgun sequence.
ACCESSION   DAORVN010000136 DAORVN010000000
VERSION     DAORVN010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3617
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21073_length_3617_cov_3.503930"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1458
                     /gene="murJ"
                     /locus_tag="PKV23_03770"
     CDS             <1..1458
                     /gene="murJ"
                     /locus_tag="PKV23_03770"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HQX84586.1"
                     /translation="TGTVAQAFFVAQRFPNMFRALFAEGAFNSAFVPQFARRIEGEGQ
                     SAAHKFAIEIFSALLAWLLVFTAVAMIAMPWLIYVIAPGFSQDAQKLQLTIDLTRICF
                     PYLLFMSLTALQSGVLNSLNRFSAAAAAPILLNIFMILATVVAWIFETGDSALTGYIL
                     AAGVSMAGVAQFLLLAFVCRRAGMPLVPQWPVFSPDVKRVLALSIPGIISGGIMQINL
                     LVATVIATSIDRAVAYLYYADRLYQLPLGVIVVAIGVVLLPDLSRKLRAGDDRAALNS
                     QNRALELSLFLTVPAAIALMAIPVPIIRTVLEHGAFTAADTLAVAPTVLAFATALPAF
                     AMTKVFQPGFYAREDTRTPMRFAVISVAINIAASLLLSRWFGHVGIAAATSIAAWANA
                     ILLATALTRRGHYRLDDRLKARLPKILLCAVAMGLLLMLGLWFLEENFTESASFSSAA
                     WGLLVLIAGGGASYFALAHFSGAMSLAEMRAVTKR"
     gene            1522..2559
                     /gene="trpS"
                     /locus_tag="PKV23_03775"
     CDS             1522..2559
                     /gene="trpS"
                     /locus_tag="PKV23_03775"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018182257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HQX84587.1"
                     /translation="MTTFTPRVFSGMQPTGNLHLGNYLGAMLNWIKLQETHESIYCVV
                     DMHAITLWQEPKELHKSILEVTAAYLACGLDPKRSIIFNQSQVQEHAELAWIFNCVAR
                     MGWLNRMTQFKEKAGKDKEQASVGLYVYPNLMAADILAYKATHVPVGDDQKQHLELAR
                     DIAGKFNLDFGDAISAAGYKGDFFPQPEPFIIGPATRVMSLRDGAKKMSKSDPSDLSR
                     INLSDDAETIARKIRKAKTDTDGLPSEAAGLKGRPEAENLVGIHAALSGQSVEQVLGQ
                     FGGGQFSRLKEALAELSVAKLGPIGQETRRLKGDSGYVEGILADGSARARAIAGPILR
                     ETRKIVGFVVS"
     gene            2621..3181
                     /locus_tag="PKV23_03780"
     CDS             2621..3181
                     /locus_tag="PKV23_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006615438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HQX84588.1"
                     /translation="MFIQTEATPNPATLKFIPGKPVLPGSTREYRAADEADDCALAQQ
                     LFTVSGVSGVFLGQDFITVSKSDGEWQHLKPAILGHIMDHYLSSAPMLKGDKSQAMTD
                     SGEFFDESDKAIVGTIKELLETRVRPAVAQDGGDITFHGFKEGVVFLNMKGSCAGCPS
                     STATLKHGIENLLRHFVPEVTEVRPV"
     gene            complement(3184..>3617)
                     /locus_tag="PKV23_03785"
     CDS             complement(3184..>3617)
                     /locus_tag="PKV23_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007633805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HQX84589.1"
                     /translation="MCDFIIAADTAKFGLPEITLGIIPGSGGTQRLARSIGKAKAMEM
                     ILTGRLMEAAEAERCGLVSRIVAPDKLLDEAMETAAKIAAFSQPIAMMAKEAVNRAFE
                     TSLAEGLRFERRLIHSMFALEDQKEGMSAFVEKRKPAFRNR"
BASE COUNT          691 a         1117 c         1043 g          766 t
ORIGIN      
        1 accgggaccg tcgcgcaggc attttttgtg gcccagagat ttccaaacat gtttcgcgcc
       61 ctgtttgccg aaggggcttt caattccgcc tttgtgccgc aatttgcgcg gcgcatcgag
      121 ggcgaagggc aaagtgcggc gcacaagttt gcaattgaaa tattctcggc cctgctggcc
      181 tggcttctgg tgttcacagc cgttgcgatg atcgccatgc cgtggctcat ctatgtcata
      241 gcaccaggtt tttcgcagga cgcccagaag ctgcaactga ccattgatct cacgcggatc
      301 tgctttccct atctgctctt catgtcgctc acggccttgc agtcgggggt gctcaacagc
      361 ctcaaccggt tttcggcggc ggcggcggcg cccattctgc tcaacatatt catgatcctc
      421 gcaaccgttg tggcatggat attcgaaacg ggcgacagcg cgctcaccgg atacatcctc
      481 gctgccggcg tctccatggc gggggtcgcg caattcctgc tcctggcatt tgtctgcagg
      541 cgcgcaggga tgccactggt gccgcaatgg ccggtatttt cgcccgacgt caaacgggtg
      601 ctggcgctct cgatacccgg catcatttcg ggcggaatca tgcagatcaa cctgctcgtc
      661 gccaccgtca tcgccacgag catcgaccgc gcagtggcct acctctacta tgcggaccgg
      721 ctttatcagc ttccgctcgg cgtcattgtc gtggcaatcg gcgtggtctt gctgccggat
      781 ctgtcacgca agctccgcgc gggcgatgac cgcgccgcgc tcaacagcca gaaccgggcg
      841 ctggagctct cgctgtttct caccgttccc gcggcgattg ccctgatggc cattccggtg
      901 ccaatcattc gcacggttct cgaacatggc gcattcaccg cggctgacac gcttgccgtg
      961 gcccccacgg tgctggcctt tgcaacagca cttccggcct ttgccatgac caaggttttc
     1021 cagcccggct tctatgcccg ggaagacacc aggacaccga tgcgttttgc ggtcatttcg
     1081 gtggcgatca atatcgcggc gagcctgctg ctgtcgcgct ggttcggcca tgtcggcatt
     1141 gcggctgcaa catccatcgc cgcctgggcc aatgccatcc ttctcgccac cgcattgacg
     1201 cggcgcggcc attaccgcct ggatgaccgc ctgaaggcca ggctgccgaa aatccttctg
     1261 tgcgccgtcg caatggggtt gctgctgatg cttggcctgt ggttcctgga ggagaatttc
     1321 accgaatccg caagcttctc cagcgcggca tggggattgc ttgttctcat agctggcggc
     1381 ggcgccagct actttgccct ggcccatttc tcaggcgcca tgagccttgc cgaaatgcgt
     1441 gcggtcacaa aacgctaagt cttttcaagc cgtgcaattg cgggcataag gcggcccagt
     1501 tcagaccaca ggatttcctt tatgaccact ttcaccccac gggttttctc cggcatgcag
     1561 cctacgggca atttgcacct cggcaactat ctgggcgcca tgctgaactg gatcaaactc
     1621 caggaaaccc atgagtccat ttattgcgtg gtcgacatgc atgccatcac cctgtggcag
     1681 gaacccaagg aactgcacaa gtccatcctt gaagtgactg ccgcctatct cgcctgcggc
     1741 ctggacccga aacgctcgat cattttcaac cagagccagg tgcaggaaca tgcggaactt
     1801 gcctggattt tcaactgcgt ggcacgcatg ggctggctca accggatgac ccagttcaag
     1861 gaaaaggccg gcaaggacaa ggagcaggct tcggtggggc tttatgtcta tcccaacctc
     1921 atggcggcgg atatcctggc ctacaaggca acccatgtgc cggtcggcga tgaccagaag
     1981 cagcatctgg aactggcgcg cgatatcgcg ggcaagttca acctggattt cggcgatgcg
     2041 atcagcgccg ccggctacaa gggcgatttc ttcccgcagc ccgagccctt cattatcggg
     2101 cccgccaccc gggtcatgtc gctgcgcgac ggggcaaaaa aaatgtcgaa gtcagaccct
     2161 tcggacctgt cgcggatcaa cctgtccgac gatgccgaga cgattgcccg caaaatccgc
     2221 aaggccaaga ccgatacgga cggcctgcct tcggaggcgg caggactcaa gggccggcca
     2281 gaggctgaaa acctggttgg catccatgcg gccctgtcgg gccagtcggt ggagcaggtt
     2341 ctggggcagt tcggcggcgg gcagttttcc cgcctgaagg aggccctggc ggagctgtca
     2401 gtggccaagc ttggacccat tggccaggaa acccggcgtt tgaaggggga ttccgggtat
     2461 gtcgagggca ttttggccga tggctcggcc cgggcgaggg ccatagcggg gccaatcttg
     2521 cgggagacac gtaaaattgt cggttttgtc gtctcctgag acaccgcaac agtttgaaat
     2581 gattccaaac tagcctatat agaggggcag aggacaaagc atgtttattc agactgaagc
     2641 caccccaaac cctgccaccc tgaagttcat tccgggaaaa cccgtgctgc ccggctcgac
     2701 gcgcgaatac cgcgcggcgg acgaggcgga cgattgtgcc ctggcccagc aactgttcac
     2761 cgtttccggc gtttccggcg tcttccttgg tcaggatttc atcacggtca gcaagagcga
     2821 cggcgaatgg cagcacctga agcccgccat cctcggccat atcatggatc actatctgtc
     2881 gagcgcgccc atgctgaagg gcgataaatc ccaggccatg accgacagcg gcgagttctt
     2941 tgacgagtcg gacaaggcca tcgtcggcac cattaaggaa ctgctggaaa cccgcgtccg
     3001 gccagccgtt gcccaggacg gcggcgatat tacctttcat ggcttcaagg aaggcgtggt
     3061 ctttctcaac atgaagggct cgtgcgccgg ctgcccgagt tccaccgcca cgctcaagca
     3121 cggcattgag aacctgctgc ggcatttcgt tccggaagtc accgaggtcc ggccggttta
     3181 agtctaccgg ttcctgaatg cgggtttccg cttttcaaca aaggcggaca tgccttcctt
     3241 ctgatcctcc aatgcgaaca tggaatggat gagtcggcgc tcaaagcgca ggccttcggc
     3301 aaggctcgtt tcaaaagcac gatttactgc ctctttcgcc atcatggcta tgggctggga
     3361 aaaggctgca atttttgcgg ccgtctccat cgcttcatcc agcagcttgt cgggggccac
     3421 aatgcgcgac accagcccgc aacgctccgc ctcggcagct tccatcaaac ggcccgtcag
     3481 gatcatttcc atggctttag ccttgccgat ggagcgggcc agcctttgcg tgccgccgga
     3541 acccggaata atccccaagg tgatttccgg caggccgaat ttcgccgtgt cggcggctat
     3601 gatgaaatca cacatca
//
LOCUS       DAORVN010000137         3605 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_21194_length_3605_cov_2.928169, whole genome shotgun sequence.
ACCESSION   DAORVN010000137 DAORVN010000000
VERSION     DAORVN010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3605
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21194_length_3605_cov_2.928169"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..860)
                     /locus_tag="PKV23_03790"
     CDS             complement(<1..860)
                     /locus_tag="PKV23_03790"
                     /inference="COORDINATES: protein motif:HMM:NF018827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I poly(R)-hydroxyalkanoic acid synthase"
                     /protein_id="HQX84590.1"
                     /translation="MSDSTPQKPGDFKLTDPVQLAQNLARVFEQLAHIARQISERPEL
                     AKKQAETQVTPLDQVTKTLGAVAQAYMAEPQKLMEAQTALWNSYSQLWQNAWSTVLGT
                     PTEPMASPARNDKRFKDRDWQENTVFDFLKQFYLITANWAQDFVKNADGLDEHTRQKA
                     KFYVEQILNAVSPSNFPVSNPEILRATLGSNGANLIEGLKHLEQDLATPDGRLRIKQS
                     DTTAFELGKNIAMTPGKVVFRNDTFELIQYSPSVQRAYEIPLLIVPPWINKFYILDLN
                     PKKSFVRWAT"
     gene            999..2303
                     /locus_tag="PKV23_03795"
     CDS             999..2303
                     /locus_tag="PKV23_03795"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019995100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HQX84591.1"
                     /translation="MTNALRLGIAGLGNVGVGVLDIVKKHGALLADRAGVPIKVTAVA
                     ARRKGKDRGHDLSGIKWHDDPVALARSLDIDVFVELMGGEGDPAKAAVEAAIAAGKHV
                     ITANKALLAHHGAALARAAEAKGVALNFEASVAGGIPIIKALREGLIGNRVSKLFGIM
                     NGTCNYILTKMANEGRSFMDVLKEAQNLGYAEADPTFDIGGFDTAHKLAILTSLAFGT
                     EVNLDAIEIEGIEAITLDDIRNASDMGYKIKLLGVAIAHAEGIEQRVNPTLVPKGAPV
                     SDTDGVFNAIVVKGDFVGDLMLEGRGAGAHPTASAVLSDVVDIARGNRRPAFGIPAAK
                     LTPYKQSPKKAHEGGYYVALELVDRPGAVASIAQILADEKISIESIVQRGRQGQETKR
                     ATATFILITHDTLETSIRTALQQIEKNGHVASKPRVIRIERL"
     gene            2300..>3605
                     /locus_tag="PKV23_03800"
     CDS             2300..>3605
                     /locus_tag="PKV23_03800"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HQX84592.1"
                     /translation="MTERNFLDVRRSLSGRRWIARLQDERLAQAIAERYELPEILGRV
                     MAARGVGLEDAEAFLNPTIRSLMPQPSALKDMEKGAARLAEAIIAQTRIGIISDYDVD
                     GVSSAAIFQLFLRSVGSGAIVHVPDRLTEGYGPSEHAVKLLKEQGCDLLLTLDCGVLA
                     HDPLAHAAELGMETIIVDHHQAGEILPEAHAVINPNRQDDVSGFGYLCAAGVVMILIA
                     TVNKLLRAMGWYSAERPEPNMLQWLELVALATVCDVVPLKGLNRAYVTQGLKIMSRRE
                     NLGLAMLSDIARLKRSPDVYALGFVLGPRLNAAGRIGHADLALELLTTRDKGRANQLA
                     QELEQLNRQRQAIEMRVVDAAALQAEASLGKEHRSSVIVVTNDNWHPGVVGLAAARLK
                     ERFQLPSFVLASNPKTGFASGSGRSIAGVDIGSAVRIALEAGV"
BASE COUNT          704 a         1097 c         1058 g          746 t
ORIGIN      
        1 tcggttgccc agcgcacgaa tgacttcttg gggttgaggt caagaatgta aaacttgttg
       61 atccacggcg gcacaatgag caggggaatt tcataggcgc gttgcaccga cggggaatac
      121 tggatgagtt cgaaggtgtc gttgcggaac acgaccttgc ccggcgtcat ggcaatgttc
      181 ttgccgagtt cgaaagctgt cgtgtcgctc tgtttgatgc gcagccgtcc atccggcgtg
      241 gccaggtcct gttccaaatg tttcaggcct tcgatgaggt tcgccccgtt gctgcccaac
      301 gtggcacgca ggatttcagg attcgagacc ggaaaattgg aaggtgaaac cgcgttgagg
      361 atctgttcca catagaattt tgccttctgg cgcgtgtgtt catcaagccc atcggcgttc
      421 ttgacgaagt cctgcgccca gttggcggta atcaggtaga actgcttgag aaaatcaaaa
      481 accgtgtttt cctgccagtc ccggtccttg aagcgcttat cgttgcgcgc cgggctggcc
      541 atgggctcgg taggcgtgcc aaggacagtc gaccaggcgt tctgccagag ctggctataa
      601 ctgttccaca gcgctgtttg ggcttccatc agcttctgtg gttcggccat ataggcctgc
      661 gccacggcgc caagcgtttt ggtgacctgg tcgagggggg tgacctgggt ttccgcctgc
      721 ttcttggcca gttccgggcg ttcggatatc tgccgggcaa tatgcgctaa ctgctcaaat
      781 acccgcgcca gattctgggc aagttggaca gggtccgtca gcttgaagtc gcccggcttc
      841 tggggggtgg aatcgctcat caatcctgtc tgttctttgt tgccggcagt ttaatggtgg
      901 aggcccggaa cgccaacaat aaacagcctt tcttgcaaaa tcatgtctgt ctaggcataa
      961 gacaaaccac ccttaacctg aaagccccgg cggacaatat gactaacgct ttgcgtctcg
     1021 gaatagccgg cctcggcaat gtgggcgtgg gcgtgctcga tatcgttaag aagcatggcg
     1081 ccctgctggc cgaccgggcc ggggttccga tcaaggtgac tgccgttgcc gcccgccgca
     1141 aaggcaagga ccggggtcat gacctttccg gcatcaaatg gcatgatgac cccgtggccc
     1201 ttgccagatc cctcgatatc gatgtcttcg tcgaactcat gggcggcgaa ggcgatccgg
     1261 ccaaggcagc cgtggaagca gccatagcgg cgggcaagca cgtcattacc gccaacaagg
     1321 ccttgctggc ccatcatggc gcggcccttg cccgggccgc cgaggccaag ggtgtcgccc
     1381 ttaatttcga ggcctccgtc gccggcggca ttcctattat caaggcgctc cgcgaaggcc
     1441 tgatcggcaa ccgcgtcagc aagctcttcg gcatcatgaa cgggacctgc aactacattc
     1501 tcacgaaaat ggccaatgag ggtcgaagct tcatggacgt tctgaaggag gcgcagaatc
     1561 tcggctacgc cgaagccgat ccaaccttcg acatcggcgg ctttgatacc gctcacaaac
     1621 tggcgatttt gacttcgctg gccttcggaa ccgaggtgaa cctggatgct attgagatcg
     1681 aaggcattga agccatcact ctggatgata tccgaaacgc cagtgacatg ggctacaaga
     1741 tcaagttgct cggcgtcgcc attgcccacg ccgaaggcat tgagcagcgc gtcaatccca
     1801 ccctcgttcc caagggcgcg cccgtctcgg acaccgatgg cgtattcaac gccatcgtcg
     1861 tgaagggcga tttcgtcggc gatctcatgc tggaaggcag gggtgcgggc gcccatccta
     1921 ccgcttcggc ggttctttcc gacgttgtcg atatcgcgcg cggtaaccgg cgtccggcct
     1981 tcggcattcc cgccgccaag ctcacacctt acaaacagtc gccgaagaag gcgcacgagg
     2041 gcggctatta cgtggccctt gaactcgtgg accgccccgg tgcggtggct tccatcgcgc
     2101 aaattctggc tgatgaaaaa atctccattg aaagcatcgt ccagcgtggc cgccagggcc
     2161 aggaaaccaa acgggccacc gcaacattca tcctgataac tcacgacacg ctcgagacct
     2221 ccatccgcac tgcgcttcag cagatcgaga agaacggcca cgttgcctca aagcctcgcg
     2281 tcatccgcat agagcggctg tgacggagcg caatttcctt gacgtacggc gttcattgag
     2341 cggcaggcgc tggatcgcgc gcctgcagga tgagcgcctg gcccaggcca ttgccgaacg
     2401 atatgaactg ccggaaattc tcggccgcgt catggcggcg cgcggtgtgg gacttgaaga
     2461 tgctgaagcc ttcctcaatc caacgatccg cagcctcatg ccgcagcctt cggccctgaa
     2521 ggatatggag aagggggcgg cgcgacttgc cgaggcaatt atcgctcaaa cccgcatcgg
     2581 gatcatcagt gactatgatg tcgatggcgt ttcctccgct gcaatcttcc agttgttcct
     2641 tcgctccgtg ggctccggcg ccatcgtcca cgtgcccgac cggctgaccg aaggctatgg
     2701 ccccagcgaa cacgcggtga aacttttgaa agagcagggc tgcgacctgc tgctcaccct
     2761 ggattgcggt gtccttgccc atgatcctct ggcccacgcc gccgagctcg gcatggaaac
     2821 catcatcgtc gaccatcatc aggccggcga aattctccca gaggcccatg cggtgatcaa
     2881 tcccaaccgc caggatgatg tttccggctt cggttatctc tgtgccgccg gggtggtgat
     2941 gatcctcatc gccaccgtca acaaattgct gcgcgccatg ggctggtact cagccgaacg
     3001 ccccgaaccc aacatgttgc aatggctcga gctcgtggcc cttgccactg tctgcgacgt
     3061 cgtgcccctg aagggcctta accgcgccta tgtcacgcaa gggttgaaga tcatgtcacg
     3121 ccgcgagaat cttggccttg ccatgctgag cgacatcgcc cgcctgaagc gcagccccga
     3181 tgtctatgcc ctgggttttg tgctcggccc gcggctcaac gcggcagggc gcatcggcca
     3241 tgccgacctg gcgcttgagc ttctcaccac gcgcgacaag ggccgggcca accaactggc
     3301 ccaggaactc gagcagctta accgccagcg ccaggccatc gaaatgcgcg tcgtcgatgc
     3361 cgccgcactt caggctgaag catcgctcgg caaggaacat cgcagttcgg tgatcgtcgt
     3421 gaccaatgac aattggcatc ccggcgtcgt cggattggct gcggcccggc tcaaggaacg
     3481 ctttcaactg ccgagctttg ttctcgccag caatccgaag acgggttttg ccagcggctc
     3541 gggtcgctcc attgctggcg tcgatattgg cagcgccgtc cggatcgcac ttgaagccgg
     3601 cgtca
//
LOCUS       DAORVN010000138         3604 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_21208_length_3604_cov_3.132150, whole genome shotgun sequence.
ACCESSION   DAORVN010000138 DAORVN010000000
VERSION     DAORVN010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3604
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21208_length_3604_cov_3.132150"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1133)
                     /locus_tag="PKV23_03805"
     CDS             complement(<1..1133)
                     /locus_tag="PKV23_03805"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS-domain containing protein"
                     /protein_id="HQX84593.1"
                     /translation="MSDNPDEKERLRRLAQYRISRTPPEAAFDDITRLLAVLFNAPIA
                     YLCITEEHCHWFKSRIGIELEEIPRAISFCDHTVKQEGVLVVPDAARDERFAASSMVT
                     GPLAIRFYAGVTLRDADGFAMGTLAVADTVPHGVTEQQKDSLERLANIALGRMELRKT
                     QAELRETVAVTETARQHAVSDHAELRQVIECLPEAIVLLDAQNRLLLWNRNYELMFPD
                     TAPYMKPGVSIETIYRLALDRGRQVTRMDAAAEEAWIKNRLERLDRGDSIVEQNFGDR
                     RWIRYDQHRAPDGKKIFVRTDITDDRNAAESFRLLFENNPVPMWVVEKATLKFIDINA
                     AAVALYGYTRDQFLKMTSLEIHPASEYQRALEDAKSDFRLDSG"
     gene            complement(1299..2000)
                     /locus_tag="PKV23_03810"
     CDS             complement(1299..2000)
                     /locus_tag="PKV23_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008837483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HQX84594.1"
                     /translation="MSRLHLHPESHLVSRIGWLRAAVLGANDGIVSTASLIVGVAAAS
                     AASSEILVAGVAGLVAGAMSMAAGEYVSVSSQSDTEGSDLARERAELASQPDFERDEL
                     AQIYVKRGLEPKLAQKVADQLMAKDALGAHARDELGISEVTAARPLQAALTSAATFAI
                     GAAMPLLIVIVAPPSAIVPVVSLASLAFLALLGAVGARAGGADVTTATLRVTFWGAMA
                     MALTAGIGALVGTAV"
     gene            complement(1997..2401)
                     /locus_tag="PKV23_03815"
     CDS             complement(1997..2401)
                     /locus_tag="PKV23_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012115916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HQX84595.1"
                     /translation="MLEYTVTAQRSDSHGSEARTKDAVLTLDTDVNGRPDAFNPAELF
                     LASIAACMIKGIERMIPLLKFQLRGVEVKLHGVRQDSPPKMISIDYELIVDTEEDDRR
                     LALLHANVRKYGTISNTVAAAAKLEGTIRRKQ"
     gene            2605..3318
                     /gene="rph"
                     /locus_tag="PKV23_03820"
     CDS             2605..3318
                     /gene="rph"
                     /locus_tag="PKV23_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006724974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HQX84596.1"
                     /translation="MRPSGRKPDQLRAVSFERNYIKHAEGSCLVRFGDTHVLCTASLE
                     ERVPPWMKGLGKGWVTAEYGMLPRSTGDRMRREASAGKQSGRTQEIQRLVGRSLRAVV
                     DLVALGEKQITLDCDVIQADGGTRTAAITGSWIALHDCLKWMEARSMFKTWPMKDHVA
                     AISCGIHGDGVVLDLDYEEDSSAGTDANFVMTGSGGIVEVQGTAEGEPFSQERFLELM
                     GLARKGIDELVAMQKAAIA"
     gene            3315..>3604
                     /locus_tag="PKV23_03825"
     CDS             3315..>3604
                     /locus_tag="PKV23_03825"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HQX84597.1"
                     /translation="MIKARHILETVLYAEDLSAARRFYGEVLGLPIYQEVDGRLVFFR
                     CAAQMLLLFNPKLSGSQRSDKGPPAHGTKGNGHVCFRATVEELKVWQKHFES"
BASE COUNT          692 a         1077 c         1096 g          739 t
ORIGIN      
        1 tcaccactgt ccaatcggaa atcggacttg gcgtcttcca gcgccctctg gtattcgctg
       61 gcggggtgta tctccagcga cgtcattttt aggaactggt cgcgcgtata gccataaagt
      121 gccacagcgg cagcattgat atcgatgaat ttcaaggttg ctttttcaac gacccacatg
      181 ggaaccggat tgttctcgaa cagcaggcgg aaggattcgg ccgcgttcct gtcgtcggta
      241 atgtccgtcc tgacaaagat cttttttcca tcaggggccc ggtgctggtc gtagcggatc
      301 cagcgccggt cgccaaagtt ctgttcgaca atcgaatccc cccggtcaag gcgctccagc
      361 cggttcttga tccatgcctc ctcggcggcg gcatccatcc tggtgacctg gcgccccctg
      421 tccagggcca ggcggtagat cgtttcgatg ctgacgcccg gcttcatgta gggcgccgtg
      481 tcagggaaca tcagttcata gttccggttc cagaggagaa gccggttctg cgcgtcgagc
      541 aggacaatgg cctccggcag gcattcgatg acctggcgga gctcggcatg gtctgagacc
      601 gcgtgctggc gcgccgtttc ggtcaccgca accgtttcac ggagttcggc ctgggttttc
      661 cgcagctcca tgcggccgag cgctatgttt gccagccttt ccagcgaatc cttctgctgt
      721 tcggtgaccc catgcggcac ggtatcggca acggccaggg tgcccatggc aaagccgtcg
      781 gcatcgcgca gcgtaacgcc cgcatagaag cggatggcaa gcggacccgt caccatggag
      841 cttgccgcga acctttcgtc gcgcgctgca tcgggcacca ccaggacgcc ctcttgcttc
      901 acagtatggt cgcagaagct gatggcgcgg ggaatctcct ccagttcaat tccaatgcgg
      961 gacttgaacc agtggcagtg ttcttcagtg atgcaaagat aggctatggg ggcattgaac
     1021 aggacggcca gcaacctggt gatgtcatca aatgccgctt ccggaggggt gcgcgaaatc
     1081 cggtattgcg cgaggcggcg gagcctttct ttttcgtcgg ggttgtcgct cataggttta
     1141 ctccgtccct ggcaacaagg cttctgcgag ccagctccct tggccgcgac tctgacgcag
     1201 aaaattttat gacctgttaa cggtaactcc caggcggctg acttccatcc gatggttact
     1261 cgctagtgaa ttttccggct ggacccgcac tgaaactgct acacagcagt accgactagc
     1321 gccccgattc cagcagtcag ggccatcgcc atcgcgcccc aaaatgtgac gcgcagcgtt
     1381 gccgtggtga catcagcgcc cccagcccgc gcccccaccg cgccaagcag cgccagaaat
     1441 gccagggagg caagtgaaac gacggggact atggcggatg gcggcgctac gataacaatc
     1501 aaaagtggca tcgccgctcc aatggcgaac gttgcggcgg aagtaagcgc tgcctgcagg
     1561 ggacgcgccg ccgtcacttc cgaaattcca agttcgtcac gcgcgtgggc gccaagcgca
     1621 tccttcgcca tcaactggtc ggcgactttc tgggcgagct ttggttccag cccccgtttc
     1681 acgtagatct gggcgagttc gtcgcgctca aaatccggct ggcttgccag ttcggcccgt
     1741 tcgcgcgcca ggtcggatcc ttccgtgtcg gattgcgaac tgaccgagac atattcgcct
     1801 gccgccatcg acatggcccc ggcaaccagt ccggctacac ctgccaccag gatttccgat
     1861 gacgcggccg atgccgctgc gacgccgaca atcaggcttg ctgtcgaaac gataccatca
     1921 ttcgccccga gcacggcggc gcgcagccag ccgatgcgcg agaccagatg gctttccgga
     1981 tgcaggtgaa gccggctcat tgtttcctcc tgatggtgcc ttcaagtttt gccgccgccg
     2041 caacggtgtt tgagatggtg ccatacttgc ggacgttggc gtggagcaaa gcgagacgcc
     2101 ggtcatcttc ttcagtatca acaatcagtt catagtcgat ggaaatcatt ttaggcgggc
     2161 tgtcctgccg cacgccgtga agcttaacct cgacgccgcg cagctggaac ttcagcagcg
     2221 ggatcatccg ctcgatgccc ttgatcatgc aggcggcgat gctagcgaga aacagttccg
     2281 ccggattgaa ggcatcgggc ctgccattca cgtccgtatc cagggtgagc accgcatcct
     2341 tggtccgggc ttcgctgcca tgactatcgc tgcgttgggc agtcacagtg tattcgagca
     2401 taaggctggc ctcctttggc aaggatccat agtattcagg ccgccgccgg aacggcttga
     2461 tgaagatcaa taatgcggca tggaagcgcc gtcaaaagcc tgctttgccc cgcccagggc
     2521 gtccccccgc tgccttcgtt tattgccatt tccatttgct gtcatggcgc gctagaagac
     2581 ccgaaatcac aaaaggaatg acccatgcgc ccctcaggcc gtaagcccga ccaactccgc
     2641 gccgtcagtt ttgaacggaa ctacatcaag catgcggagg gctcgtgcct ggtgcgtttc
     2701 ggcgacaccc atgtgctgtg cacggcaagc ctcgaggagc gggtgccgcc gtggatgaag
     2761 gggctgggca agggctgggt tactgccgaa tacgggatgc tgccgcgctc caccggggac
     2821 cggatgcggc gggaggcctc ggccggcaag caatcgggcc gcacccagga aatccagcgg
     2881 ctggtggggc gctcgctccg cgccgtggtt gaccttgtgg cgcttggcga aaagcagatc
     2941 acgctggatt gcgatgtgat ccaggccgat ggcggcacgc gcaccgccgc catcacggga
     3001 agctggatcg cgctgcatga ttgtttgaaa tggatggagg cgcggtccat gttcaagacc
     3061 tggccgatga aggaccatgt ggcggctatt tcctgtggca tccacggcga cggcgtggtg
     3121 cttgacctgg attacgagga agactcttca gcgggcaccg atgcaaattt tgtaatgaca
     3181 ggctcgggcg ggatcgtcga agtacagggc acggcagagg gtgagccctt cagccaggag
     3241 cgctttcttg agctcatggg cttggcccgc aagggcatcg acgaactggt ggcgatgcag
     3301 aaggcggcaa ttgcttgatc aaggcgcgcc acatacttga aacggttctc tacgcggagg
     3361 atttatccgc ggcgcgacgc ttctatggtg aggtgctggg ccttcccatc tatcaggaag
     3421 ttgacgggcg gctggttttc ttccgctgtg cggcgcagat gctgctgcta ttcaatccca
     3481 agttatcggg cagccagcgc tccgacaagg gcccgccagc ccatggcaca aagggcaacg
     3541 gccatgtctg ctttcgtgcc accgtggaag aactgaaagt ctggcaaaaa catttcgaat
     3601 ctca
//
LOCUS       DAORVN010000139         3604 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_21212_length_3604_cov_2.655396, whole genome shotgun sequence.
ACCESSION   DAORVN010000139 DAORVN010000000
VERSION     DAORVN010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3604
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21212_length_3604_cov_2.655396"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="PKV23_03830"
     CDS             <1..291
                     /locus_tag="PKV23_03830"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009451540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HQX84598.1"
                     /translation="DEITRKAAWPDWRLPKVMIEREAKKGHVLPAFMAGGPENPLGAR
                     ALYIGDTEYRIHGTTQPWSIGRAVSSGCIRMMNHEVIDLFNRVEIGATVVVE"
     gene            396..1721
                     /locus_tag="PKV23_03835"
     CDS             396..1721
                     /locus_tag="PKV23_03835"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing M23
                     family metallopeptidase"
                     /protein_id="HQX84599.1"
                     /translation="MRVDSSAPKGLSKFNILASAALAVMLAACSNSVERFADNSSSGN
                     PSDADPVYTASVPKKRTTYAPSYESSGDDKIISRPLVSAPLGAPNYPKNNYTYQKPAY
                     KQPSYSEPTVVGQNSTTIPKGGTVRVEQGMTLYSISRANGVTVAQLAAANGISPPYTV
                     STGRVLRIPGVAQARAPKPSFEPQDDFASDEPVKKASGGRHTVRGGETLFSLGRKYGV
                     SPYAIADLNDLPHDQTLNVGQSLRLPGGTAKSIVSKVPAPDEGEQIASADEPIETDDI
                     EQNVALKKPALDMDDEVPVVKPVQQQVAAVDAAPTMGSMRWPVKGKIISDYGAKPNGL
                     KNEGINIAVPEGTGVRAAESGVVAYAGNELKGYGNLVLIRHEGGWVTAYAHAKELFVK
                     RGDIVKRGDVIAKAGQTGSVSSPQLHFEVRKGATAMDPLKFLASTTASN"
     gene            complement(1774..2640)
                     /locus_tag="PKV23_03840"
     CDS             complement(1774..2640)
                     /locus_tag="PKV23_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020178463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQX84600.1"
                     /translation="MVTKSQDTWIARIADALDRLAPLPPKPGNLGRSSAFVWHASPPA
                     LEAVAKVNRVDLQLLKGIDQTRDTLLENTRRFAQGLPANNALLWGARGMGKSSLVKAA
                     HHLVNEEHKGMLKLIEIHREDIESLPALMSILRSSPKLHFIVFCDDLSFDGQDTSYKS
                     LKAALDGGIEGRPENVILYATSNRRHLLPREMIENERSTAINPSEAVQEKVSLSDRFG
                     LWLGFHNCSQDEFLDMVDGYAAHYRLPVTEAELHAGAIEWSVTRGSRSGRVAWQYIQD
                     VAGKTGTKLTPK"
     gene            2763..3101
                     /gene="yajC"
                     /locus_tag="PKV23_03845"
     CDS             2763..3101
                     /gene="yajC"
                     /locus_tag="PKV23_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002711686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HQX84601.1"
                     /translation="MFVTPAFAQSAGSPGMGDFMGMILPLVMIMAVFWFLLIRPQQRK
                     MKEHQDTISKISRGDTVVTSGGLIGRVAKVVDDKELLVEVGENVKVRVLRQGVSDVRA
                     KGEPAKAETK"
     gene            3113..>3604
                     /locus_tag="PKV23_03850"
     CDS             3113..>3604
                     /locus_tag="PKV23_03850"
                     /inference="COORDINATES: protein motif:HMM:NF019187.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HQX84602.1"
                     /translation="MLNFSRYKTIAILASVVIAIIFALPNVLSPAAQAVMWNTLGARP
                     MTLGLDLQGGSNVVMEVDRKDLRDQLTLQLSGDIRASLREAKIGYKGITRTDNGVTVR
                     LSDIADQARARTALQTLQQPQTGGLMSTGIAVNLFDLNPEGEAFNFTFSEPGLDAKVA
                     IAIG"
BASE COUNT          773 a         1083 c         1026 g          722 t
ORIGIN      
        1 gacgagatta cccgcaaggc agcgtggcct gactggcgcc tgccgaaggt catgattgaa
       61 cgcgaggcaa aaaaaggtca cgtccttccc gccttcatgg cgggcgggcc tgagaatccc
      121 ctgggcgccc gggccctcta catcggcgat accgaatacc gcattcatgg caccacccag
      181 ccctggagca tcggcagggc cgtctcctcc ggttgcattc gcatgatgaa tcacgaagtg
      241 attgatctct tcaaccgggt tgaaatcggc gccactgtcg tcgtcgaata atgcagaatt
      301 attatcggga tagcccgcag aggcgctggc cataaacgct ccattaacct gaacacgcca
      361 ttatcggccg gtaagttcgt aggtggagtt cgcgtatgcg tgtcgattca agcgccccaa
      421 aagggctctc aaagttcaac attctggctt cggcggccct ggcggtaatg ctggctgcat
      481 gctcgaattc agtcgagcgc tttgccgata attcgtcatc tggcaatccg tcggatgcag
      541 atccggtcta caccgcatcg gttcccaaga aacgcaccac ctacgcgcca tcctatgaat
      601 cctccggcga cgataaaatc atctcgcgtc cgctcgtaag cgcgccgctc ggagcgccga
      661 actatcccaa gaacaattat acctatcaga aaccggccta caagcagccg agctattccg
      721 agccaactgt agtggggcaa aactcgacca cgattcccaa aggcggcacc gtacgggtcg
      781 agcagggcat gacgctctat tcaatttcgc gggccaatgg cgtgacggtt gcccagctgg
      841 cagcggccaa tggaatttcg cctccctata cggtcagcac cgggcgcgtt ctgcgcatcc
      901 ccggcgttgc gcaggctagg gcgccaaaac catcatttga gccacaggat gatttcgctt
      961 ccgatgagcc ggtgaagaag gcctctggcg gcaggcatac tgtccgcggt ggtgaaaccc
     1021 tgttctcgct tggtcgcaaa tacggcgtgt ctccttacgc aattgctgac ctgaatgact
     1081 tgccccatga ccagaccctg aatgtcggcc aaagccttcg cctgccagga ggaaccgcca
     1141 agtcaattgt ttcaaaagtt ccagcgcccg atgaaggcga gcagattgca tccgccgacg
     1201 aaccgatcga aaccgatgat attgaacaga atgtcgcgct gaaaaagccc gcgctggata
     1261 tggacgacga agtgccagtc gtgaagccgg ttcagcagca agtcgcagca gtcgatgccg
     1321 cacccaccat gggcagcatg cgctggccgg tcaagggcaa gattatttcg gattacggcg
     1381 ccaagccaaa cggcctgaag aacgaaggca tcaacattgc cgtgcctgaa ggcaccggcg
     1441 tccgtgccgc cgaaagcggc gtcgtggcct atgccggcaa cgagttgaag ggttacggca
     1501 accttgttct catccgccac gaaggcggct gggtcaccgc ctatgcccat gccaaggaat
     1561 tgtttgtgaa acgtggcgac atcgtcaagc gtggcgatgt cattgccaag gccggccaga
     1621 ccggttccgt ttccagcccg caattgcact ttgaagttcg caaaggcgca accgccatgg
     1681 accccttgaa gttcctggcg tccacaaccg catcgaacta gagtacgtaa gatcctctgc
     1741 aagcctgggc ccggcagcaa tgccgggcct tcgctatttg ggcgtgagct tggtgcccgt
     1801 ctttcccgct acgtcctgga tgtactgcca ggccacgcgc cccgagcggc tgccgcgcgt
     1861 gaccgaccat tcaatggcgc ccgcatgaag ttcggcctct gtcaccggca gcctgtaatg
     1921 cgccgcatag ccgtcgacca tgtcgaggaa ctcatcctgc gagcagttgt ggaaacccag
     1981 ccacaggcca aatctgtccg ataaggatac cttttcctgc accgcctccg atggattgat
     2041 cgccgtcgac ctttcgttct cgatcatctc gcggggcagc aggtggcgcc ggtttgacgt
     2101 cgcgtaaaga atgacgtttt caggccgccc ttcaatgccg ccatcaagcg cagccttcaa
     2161 tgatttgtat gaggtgtcct gcccatcgaa agaaaggtca tcgcagaaga cgatgaagtg
     2221 gagcttcggc gaactgcgca ggatcgacat gagggcaggg aggctttcaa tgtcttcccg
     2281 gtgaatctcg atgagcttga gcatcccctt gtgctcttcg ttcaccagat gatgcgccgc
     2341 cttgaccagc gaggattttc ccatgccccg cgccccccac agcagcgcat tgttggccgg
     2401 caggccctgg gcaaagcgcc gcgtattttc cagcagggta tcgcgcgtct ggtcgatgcc
     2461 cttgagcaat tgcaggtcaa cccgattcac tttggcaact gcttcaagtg cgggcggtga
     2521 tgcatgccag acaaaggcgc tggaacgccc gagattgccg ggttttggcg gcaaaggcgc
     2581 caaccggtcc agagcatcgg cgatgcgggc aatccaggtg tcttgggatt ttgtaaccat
     2641 gccaagctct tagcttgcaa gccgcacgcc gtaaagcgtc ctaaatccgg cgttgcaatg
     2701 gcgcaatcca tcgctatatt ccgcgcaaac cttccgtagg ccaaatcgat taggagcatc
     2761 ccatgttcgt gacccccgcc tttgcccagt ccgccggctc gcccggcatg ggagatttca
     2821 tgggcatgat cctccccttg gtgatgatca tggccgtgtt ctggttcctg ctcatccggc
     2881 cccagcagcg caagatgaaa gagcatcagg atacgatcag caagatttcc cgcggcgata
     2941 cggtcgtcac cagcggtggc ctcatcggcc gtgtggcaaa ggtcgttgac gacaaggaac
     3001 tgcttgtcga agtgggcgag aacgtgaaag tccgcgtgct gcgccagggc gtcagcgacg
     3061 tgcgcgccaa gggcgagccc gccaaggcgg aaacgaaata agttacctgt aaatgctcaa
     3121 tttcagccga tacaagacga tcgccattct ggcatcggtc gtgattgcga taatctttgc
     3181 cttgcctaat gtgctgtcgc ccgctgccca ggccgttatg tggaatacct taggggcgcg
     3241 gcccatgacg ctcggccttg acctgcaggg cggctctaac gtggtcatgg aggtcgaccg
     3301 gaaagatctc cgcgaccagc tcacgttgca gctttcaggc gatatccgcg cgagcctgcg
     3361 cgaagccaaa attggctaca aaggcattac ccgcaccgac aacggcgtta cggtgcgcct
     3421 ctcggatata gccgatcagg cgagagccag gacagcattg cagacgttgc agcagccgca
     3481 gaccggcggg ctgatgtcga cgggaattgc ggtcaacctc ttcgatctca atcctgaagg
     3541 cgaagcgttc aatttcacct tttcagaacc gggcctggat gccaaggtcg caatcgctat
     3601 cggg
//
LOCUS       DAORVN010000140         3576 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_21498_length_3576_cov_2.785288, whole genome shotgun sequence.
ACCESSION   DAORVN010000140 DAORVN010000000
VERSION     DAORVN010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3576
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21498_length_3576_cov_2.785288"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(118..1587)
                     /gene="arcD"
                     /locus_tag="PKV23_03855"
     CDS             complement(118..1587)
                     /gene="arcD"
                     /locus_tag="PKV23_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006201039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043858 - arginine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HQX84603.1"
                     /translation="MAKSVSIPVSTVRKKSIAGLGLVPLVALVVGSMIGGGVFSLPQN
                     MAKGASPGAVIIGWLIAGVGMLALAFVYQGLSTRKPALDAGPYSYARAGFGDFIGFNS
                     AWGYWLSAWIGNVSYAVLIFGALSYFVPMFGAEGNTWQAIVGASISLWLVHALILMGI
                     RQAALVNVITTIAKLAPIFIFIVAVLIAFSLPTFSIDLWGSGAPALGDVMTQVKSTML
                     VTLWVFIGIEGASVVSARAERRADIGKATILGFAVALSVYLLVSLLSFGVMTQPELAG
                     LPSSASMANVLEKAVGPWGSVLVRIGLVVSVAGAFLSWTLFAAEIPYRAAREGMMPEV
                     FARENANGSPTGSLWITNILVQIFLIITLYANSTYLALFYIASTAILVPYVFSGAYAL
                     KLALTGESYGAGNGRGRDIFVGAIATVYGIWLVYAAGPNYLLMCALLYAPGILVYWWA
                     RNVRGEKAFSVAEAAIAAGIVIAAGIAGYWMWTGAISAL"
     gene            1705..2412
                     /locus_tag="PKV23_03860"
     CDS             1705..2412
                     /locus_tag="PKV23_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009451929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQX84604.1"
                     /translation="MTVAVFSGRGLTKTYRSGEVEVHALRGADIDLYEGEIAVLLGPS
                     GSGKSTLLNILGGLDRATTGTVRFRDHDLTNASERELTQYRREHVGFVFQFYNLIPSL
                     TARENVALVTEISSNPMDPEKALGLVGLAHRLDHFPSQLSGGEQQRVAIARAIAKRPD
                     VLLCDEPTGALDSKIGVLVLEALDHVNQTLGTTTAIITHNAAIQSMAHRVFNFGDGRI
                     ISTHANETRQNPSEINW"
     gene            2451..>3576
                     /locus_tag="PKV23_03865"
     CDS             2451..>3576
                     /locus_tag="PKV23_03865"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQX84605.1"
                     /translation="MKRLWAQSLAVALVMAAGVATMLIGLGAYYSLSETRTAYYDRTQ
                     FADVFAFAVRAPKSTAAEIRQINGVAQVETRIVKAALLDIEGFELPATGYFISLPASG
                     ENTLNRLHLRSGRLPGPGSLREVAVSESFAQAHGLQIGSTLDAVLNGAKRRLTITAVV
                     LSAEFIYALGPGDMMPDDRRFGIIWMREPALAGAFDLEGAFNSVTLRLLKNAVMEDVF
                     QSLDNILERYGGEGAYGREDQLSHAFLDAELQQLSAMSRILPPVFLIVTAFLINMVLA
                     RLISLEREQVGLLKAIGYRPTEIAVHYAKLVLLISCMGIGIGFAAGTWLSHGLTVLYT
                     KFFHFPYQIFVASPKLYVVAAFVSFSAALFGSMRAVAGAAA"
BASE COUNT          793 a         1054 c         1010 g          719 t
ORIGIN      
        1 aatctctggt gggccagacc cggacggcag gtcaacaccg tccggagctt gccaatttcg
       61 gagtatgcgc cgagcggtgc tggagtggtc attagctgtt cctttccgga acccggttca
      121 gagtgcgctg atggcgccgg tccacatcca gtagccggca atacctgcgg caatcacgat
      181 gccagcagcg atggccgcct ccgccacgct gaaggctttt tcgccgcgca cattcctggc
      241 ccaccagtag accagaatgc cgggtgcata gagaagcgcg cacatcagca gataattggg
      301 gcccgccgca taaaccagcc aaatgccata aaccgtcgct atggctccca cgaaaatatc
      361 ccggccgcgc ccattgccag cgccatagct ttccccggtg agggcaagct tgagcgcata
      421 ggccccggag aaaacataag gcacaaggat ggccgtggag gcgatataga acagtgcgag
      481 ataagttgaa ttcgcataga gcgtaatgat caggaatatc tgaacaagga tatttgtgat
      541 ccacagggag cccgtgggcg aaccatttgc attctccctt gcaaagactt ccggcatcat
      601 gccttctcgc gccgcgcgat agggaatttc ggcagcaaaa agtgtccagc tcaggaaagc
      661 gcctgcgacc gatacgacga ggccgatgcg gaccagcact gaaccccagg ggccaaccgc
      721 cttttccagc acattggcca ttgaggccga acttggcagc ccggcaagtt ccggctgggt
      781 catgacgcca aaggaaagga gcgagacaag cagatacacc gagagcgcca ctgcaaagcc
      841 gagaatagtg gcctttccga tatcggcgcg gcgttcggcc cgcgccgaga cgacacttgc
      901 gccttcaatg ccgatgaaga cccacagtgt caccagcatg gtgctcttga cctgagtcat
      961 cacatcgccg agggccggtg cgccggaccc ccagaggtca atactgaacg tcgggaggct
     1021 gaaagcgatc aacaccgcaa cgataaagat gaagatcggc gccagctttg ctatcgtcgt
     1081 aatgacattg acgagtgcgg cttgccggat tcccatgagg ataagcgcat ggaccagcca
     1141 gagggagatg gaagcgccaa cgatggcctg ccaggtattg ccctcggcgc caaacatcgg
     1201 aacaaaatag ctgagggcgc cgaaaatcaa taccgcatag gagacgttgc cgatccaggc
     1261 gctcagccaa tagccccacg cgctgttgaa tccgatgaaa tctccgaacc cggcccgggc
     1321 ataagagtat gggccggcgt ccagcgccgg ctttcgcgtc gataagccct gatagacaaa
     1381 agccagcgcc agcataccaa cgccggcgat gagccaacca ataatgaccg cgccggggga
     1441 tgcccctttt gccatattct gcggcaggct gaatacacca ccgccaatca tcgaacccac
     1501 aacaagggcc acaaggggaa ccaggccaag cccagcaatt gacttcttac gcaccgtgct
     1561 gactggaatg ctgactgatt ttgccatgga tttctcctct gattccggtc actttagacc
     1621 gcaaattccg cggctacatt gatcgggatc aaggtaattg caggttctcg cgcccatctt
     1681 tcttccaagc aatgccggag gattatgaca gttgcagtat tctcaggccg cggcctgacc
     1741 aaaacctatc gcagtggcga agtggaagtg catgcattgc gtggcgccga catcgacctt
     1801 tatgagggcg agattgccgt tctgctgggg ccttccggct ctggcaagtc gacgctgctg
     1861 aacatcctgg gcggattgga ccgtgcgacc acgggcaccg tgagattccg cgatcacgac
     1921 ctgaccaatg ccagcgaacg ggaacttacg cagtaccggc gtgaacatgt gggctttgtc
     1981 tttcaattct acaatttgat acccagcctc acggcacgcg agaacgtggc cctggtgacc
     2041 gaaatttcct ccaacccaat ggaccccgag aaggccctgg gcctagtcgg tcttgcgcat
     2101 cggctggacc attttccatc ccagctttct ggcggcgagc agcaacgcgt agcgatcgcc
     2161 cgcgccattg caaagcgccc tgatgtgttg ctttgcgatg aaccaaccgg cgcattggac
     2221 agcaagatcg gcgtgctcgt ccttgaggcg cttgatcatg taaaccagac actgggaaca
     2281 acaactgcaa tcattacgca caatgctgcc attcaatcga tggcgcaccg tgtcttcaac
     2341 tttggcgacg gccggataat atcgactcac gcaaatgaaa cacggcagaa tccatcggaa
     2401 ataaactggt agcaaacatg cgagcgctcg atcgaaagct ccttcgtgac atgaagcgcc
     2461 tttgggcgca gtcgctggcc gtcgcgctgg tcatggccgc cggcgtagct acaatgctga
     2521 ttgggctggg cgcctattat tctctttcgg aaacccggac cgcttactat gaccggaccc
     2581 agtttgccga cgtttttgcg tttgccgtgc gggcgcccaa atccactgcc gccgagatac
     2641 gccagatcaa tggcgtggca caggtcgaga ccaggatcgt aaaagctgca ttgctcgaca
     2701 tcgagggttt tgaactgccc gccaccggct acttcatatc attgcctgcc agcggcgaaa
     2761 acacgttgaa caggctccac ttgcgttcag gcaggcttcc cggtccaggc agccttcggg
     2821 aagtggctgt cagcgagagt tttgcacaag cgcatggact gcaaatcggt tccacgcttg
     2881 atgcggtttt gaatggcgca aagcgccgcc tcaccatcac ggcggtggtt ctgtcagccg
     2941 agttcatcta tgcgctgggc cccggggaca tgatgccgga tgaccgccgc ttcggaatta
     3001 tctggatgcg ggagccggcg cttgccggcg cttttgacct tgaaggcgcc ttcaacagcg
     3061 tcacgctccg cttgctcaag aacgctgtca tggaggacgt gtttcaatcg ctcgacaata
     3121 tacttgaacg ctacggcggc gaaggcgcct acggccgtga ggatcaattg tcacacgctt
     3181 ttcttgacgc agagctccag caactcagcg ccatgagccg catcctgccg ccggtctttc
     3241 tcattgtgac ggcgttcctg atcaacatgg tgctcgcacg cctgatctca ctggagcggg
     3301 aacaggtggg ccttctcaag gcgataggct accggccgac tgaaattgcg gtgcattacg
     3361 caaagctggt acttctcatc tcttgcatgg ggattggcat cggctttgcg gcgggcacct
     3421 ggctcagcca tggactcacg gtgctctaca cgaagttctt ccattttccc tatcagatat
     3481 ttgtggcgtc gcccaagctc tatgtggttg cggcgttcgt ctcattttcg gcggcacttt
     3541 ttggctccat gcgggctgtt gccggggctg cggcgc
//
LOCUS       DAORVN010000141         3549 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_21801_length_3549_cov_4.103034, whole genome shotgun sequence.
ACCESSION   DAORVN010000141 DAORVN010000000
VERSION     DAORVN010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3549
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21801_length_3549_cov_4.103034"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="PKV23_03870"
     CDS             <1..286
                     /locus_tag="PKV23_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008129095.1"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HQX84606.1"
                     /translation="ARRPMHGDLSEIIHDGTGIFKGLPNRFNAGRYHSLIVETDSENS
                     EIEITARSDDGEVMGLKHKQHPTFGVQFHPESILTEHGYAMLKNFLEVVH"
     gene            283..1128
                     /locus_tag="PKV23_03875"
     CDS             283..1128
                     /locus_tag="PKV23_03875"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HQX84607.1"
                     /translation="MSVVWFNGEFVAGTLMLDPHDRGFLLGDGVFETVAVINRRPLWL
                     DEHVQRMAHAAVELGIAFSAERTFSGLTEVLKKSEAPCEVLRITLSRGKAARGLAGDG
                     ASPGLLITLDEFSVKNLFQPCRLKVSQVRRNEFAPSSRLKTLSYIDGIAAAREVAGEA
                     DDALMLNASGHVASATVANIFAMQGDELATPSLDQGVLSGITRRILLEHAKELGLKPV
                     ERVVPLEDLVRADAVFLCNSLRFIRPVTTLNGEPLGKGSLDRLIEELGGLAKKQCGTD
                     PRTLM"
     gene            1145..1798
                     /gene="fsa"
                     /locus_tag="PKV23_03880"
     CDS             1145..1798
                     /gene="fsa"
                     /locus_tag="PKV23_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008197099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HQX84608.1"
                     /translation="MKFFVDTADVVDIRDLADAGLLDGVTTNPSLIAKSGRAFKEVIA
                     EICKIVEGPVSAEVTALDSAGMIKEGNDLRKIAKNVTVKVPLTIDGLKACKALRADGA
                     MVNVTLCFSANQALLAAKAGASFISPFVGRLDDIHMEGMDLISEIRQIYDNYGFDTEI
                     LVASVRTANHIKQAALIGADVVTAPPAVLKGLAKHPLTDKGIEAFLADWKKTGQSIL"
     gene            1795..2094
                     /locus_tag="PKV23_03885"
     CDS             1795..2094
                     /locus_tag="PKV23_03885"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018183073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HQX84609.1"
                     /translation="MILEHAILDVAPGEAADYEAAVKEALPLIAATPGFIRLDIRPCL
                     ETQGRYLLLVQWRELADHTVGFRDSERYQAWKNLLHRFYSPFPLVEHFGDAVVSS"
     gene            2128..3066
                     /locus_tag="PKV23_03890"
     CDS             2128..3066
                     /locus_tag="PKV23_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HQX84610.1"
                     /translation="MSDGLQFGFSGDAAEIAVRWLQSLKSERRMAAKTLEAYARDVSQ
                     FGQFLKEHLGQPATVSDLASLTVSDFRSFMAKRRKTGVESRTLARQLSAIRSFFRFAQ
                     RNAYFANSALSALRSPKLAHSVPKPLNIQAARQVAAGDILSTDETPKWVEARDRAVLT
                     LLYACGLRISEAIGLTLSQSKSTPLVITGKGGKARIAPVLPRVREVLDSYVALCPFPL
                     KPGEPMFRGMRGGPLNARNIQLLIERMRGVLNLPDTATPHALRHSFATHLLGNGADLR
                     VIQELLGHASLSSTQIYTEVDRAHLLEQYRKAHAQS"
     gene            3105..>3549
                     /locus_tag="PKV23_03895"
     CDS             3105..>3549
                     /locus_tag="PKV23_03895"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007879477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQX84611.1"
                     /translation="MPVIDPVSSDSDFPKKTTVAVIGGGVIGVATALELSERGVPVVL
                     LEKGIIAGEQSSRNWGWCRQMGRDSREIPLILESLKLWREMNARVGAETGYRQCGVIY
                     LAENEVELAAREKWYAENAKAFGLDTKLVSGIEAERLQPGSTRPWK"
BASE COUNT          708 a          969 c         1077 g          795 t
ORIGIN      
        1 cgctaggcgc ccgatgcacg gggatttatc cgagatcatt catgatggga caggaatttt
       61 caaaggcctg cccaatcgct ttaatgcggg gcgctatcac tcactgattg tggaaacaga
      121 ttcagaaaac tccgagattg aaattaccgc gcgcagtgat gacggcgaag tcatgggatt
      181 gaaacacaag cagcatccaa ctttcggagt gcagttccat ccggagtcga tactcactga
      241 acatggttat gccatgctga agaattttct agaggtggtg cattgagtgt tgtctggttc
      301 aacggcgagt ttgttgcggg aaccctgatg cttgatcccc atgaccgtgg ttttctgctg
      361 ggcgatggtg tttttgaaac ggtggccgtg atcaaccgca ggccgctttg gcttgacgaa
      421 catgtgcagc gcatggctca tgcggcagtt gagttgggaa tcgcttttag cgcagagcgc
      481 accttctcgg ggttgacgga agttcttaaa aaaagcgagg cgccgtgcga ggttctccgc
      541 atcaccctga gtcggggcaa agcggcgcgc ggtttggcgg gtgatggcgc gtcgcccggc
      601 ctgctcatca cgcttgatga gttttccgtg aagaatttgt ttcagccctg ccgcctcaag
      661 gtctcgcagg tgcggcgtaa tgaattcgcg ccgtcgtcgc ggctgaagac gctgtcttac
      721 atcgatggga tagcagcggc gcgggaagtg gcgggagagg cggatgatgc cctgatgctc
      781 aatgcatcgg gccatgtggc ctccgccacg gttgccaata tctttgcgat gcagggcgat
      841 gaattggcta cgccctccct cgatcaagga gttctatccg gcatcacgcg gcgtattctg
      901 ttggagcatg ccaaggaact gggactcaag cctgtcgagc gcgttgttcc gctcgaagat
      961 ttagtccgtg cggatgcggt ttttttgtgc aatagcctgc gtttcatcag gccggttaca
     1021 acattgaatg gcgagccgtt gggaaagggc tcgctggaca gactgattga ggaactgggc
     1081 gggcttgcca aaaagcaatg cgggactgat cctcgcacac tgatgtgaat gaaaggaata
     1141 gattatgaaa ttttttgttg atacggctga tgtggtggac atccgcgatc ttgctgatgc
     1201 ggggcttctt gacggggtca ctaccaatcc atcgctcatt gcaaagtcgg gccgggcatt
     1261 caaggaagtc atcgccgaaa tttgcaagat tgtcgaaggg ccggtgagtg ccgaggtcac
     1321 ggcccttgat tctgcgggca tgatcaagga gggtaatgat ctccgaaaga tcgccaagaa
     1381 tgttacggtc aaggtgccgc tcaccattga cgggctcaag gcttgcaagg cgttgcgggc
     1441 cgatggggcc atggtcaatg tcacgctgtg cttcagcgcc aatcaggcac tgctggcggc
     1501 caaggccggg gcctcgttca tatcgccctt cgtcggccgt cttgatgaca ttcatatgga
     1561 aggcatggat ctcatttccg agatccgcca gatctatgac aattacgggt ttgacacaga
     1621 aatcctggtt gcctcggtgc gcaccgccaa ccacatcaag caggcagcgc tcattggggc
     1681 cgatgtagtg accgcaccgc ctgccgtcct caagggcctc gccaagcatc cgctcaccga
     1741 caagggcatt gaagcgttcc tggccgattg gaaaaagacg ggacagtcca tcttgtgatc
     1801 ctcgaacatg ccatactcga tgtcgcgccc ggcgaagcag cggactatga agcggcagtg
     1861 aaggaggcat tgcctctgat tgcggcaacg cccggtttta tccggcttga tatccggccc
     1921 tgcctggaga cgcaggggcg ctatctgctt ctggtgcaat ggcgggaact ggctgatcac
     1981 acggtgggat ttcgcgattc cgagcgttac caggcttgga agaatttgct gcatcgtttt
     2041 tacagtccgt ttccccttgt tgagcatttc ggcgatgcgg ttgtcagcag ttagaagtca
     2101 tcaaccattg ggggcctagg ttaaggcatg agcgatggtc ttcagttcgg gttttccggt
     2161 gatgcggcag agatcgccgt gcgctggctg caatcgctga aatccgagcg ccgcatggcg
     2221 gccaagacgc tggaagccta tgcccgcgat gtttcacagt ttgggcagtt cctcaaagag
     2281 catctgggcc agcccgccac cgtttctgat cttgcctccc tgacagtgtc ggatttccgc
     2341 agtttcatgg ccaagcggcg gaaaaccggg gttgaaagcc gcaccctagc ccggcagctc
     2401 tcggccatcc gttctttttt ccgctttgcc caaaggaacg cctatttcgc aaattcagcg
     2461 ctttcggcgc tgcgttcgcc aaagctggca cattccgttc ccaaaccctt gaacatccag
     2521 gcggcccggc aagtggcggc cggcgatatc ctgtccaccg acgagacgcc gaaatgggtg
     2581 gaagcccgcg accgggcggt gctcaccctg ctttatgcct gtggccttcg catatcggaa
     2641 gccatcggtc tcaccctcag ccaatccaaa tccacccccc tggtgatcac cggcaagggc
     2701 ggcaaggcgc gcattgcccc ggttctgccc cgggtgcggg aagtgctgga ctcctatgtc
     2761 gcgctctgcc cctttccgct gaaacccggc gaacccatgt tccggggaat gcgcggcggg
     2821 ccgctcaatg cgcgcaatat ccaattgctg atcgagagaa tgcggggggt tttaaacctg
     2881 cctgatacgg ccacccccca tgcgctgcgc cattcctttg ccacccatct gctgggcaat
     2941 ggggcggacc tccgggtgat tcaggagctg ctgggccatg ccagcctttc ctccacccag
     3001 atatacacgg aggtggaccg ggcccacctg cttgaacaat atcgcaaggc tcacgcgcag
     3061 agttaaggct tgttccggtg cattgctttt tgtagcaatc ggccatgcct gtcattgatc
     3121 ctgtctcctc cgattctgat ttccccaaga aaaccaccgt tgccgtcatc ggcggtggcg
     3181 tcatcggcgt tgccacggcg ctggaactgt ccgagcgcgg ggttccggtg gtgcttctgg
     3241 aaaaaggcat cattgccggt gagcagtcct cgcgcaattg gggctggtgc cggcagatgg
     3301 ggcgcgattc gcgtgaaatt ccgctcattc tagagtcgct gaagctatgg cgcgaaatga
     3361 acgcgcgcgt tggggctgaa accggctacc ggcaatgcgg tgttatttac ctcgccgaaa
     3421 acgaagtgga actggcggcg cgggaaaaat ggtacgcgga aaatgccaag gcctttggcc
     3481 tggatacgaa actggtttca gggatcgagg ccgagcgcct gcagccgggt tcgacacggc
     3541 cctggaagg
//
LOCUS       DAORVN010000142         3539 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_21911_length_3539_cov_3.276119, whole genome shotgun sequence.
ACCESSION   DAORVN010000142 DAORVN010000000
VERSION     DAORVN010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3539
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21911_length_3539_cov_3.276119"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            27..641
                     /locus_tag="PKV23_03900"
     CDS             27..641
                     /locus_tag="PKV23_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012110510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HQX84612.1"
                     /translation="MNEPKRNRNSLIYLVLLALIAGGAGIYLSGDAPREVPEVNGSSG
                     FKAFAAGSMAAFLVKADRPVVPDLAFKDSTGSGIKLNRWRGRVVLLNLWATWCAPCRR
                     EMPALNAVQQQLGSKDFEVVALSVDRKGLEASAAFLKETGADSLGLYSDETTASLTML
                     QALGLPVTLLIDRKGREIGRLLGPAEWNGAEAVAMIRAAIAEQD"
     gene            complement(638..1513)
                     /locus_tag="PKV23_03905"
     CDS             complement(638..1513)
                     /locus_tag="PKV23_03905"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018700996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HQX84613.1"
                     /translation="MKIKTVGIIGAGQMGSGIAHVCALAGFDVGVNDVSKERIDAGIA
                     TISGNLGRQLKSGKITEADRNAALKRIAPVAAYEGFADYDLVIESAVEDEQVKRKIFQ
                     ALCPHLKPHTIVASNTSSISITRLAAVTGRPEKFMGIHFMNPVPVMELVELIRGIATE
                     EATFAAVKEFTLALHKTIAVAEDFPAFIVNRILLPMINEAIYTLYEGVGGVEAIDTAM
                     KLGARHPMGPLELADFIGLDTCLSVMQVLYEGLADSKYRPCPLLVKYVEAGWLGRKSN
                     RGFYDYRGEKPVPTR"
     gene            1679..2851
                     /locus_tag="PKV23_03910"
     CDS             1679..2851
                     /locus_tag="PKV23_03910"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQX84614.1"
                     /translation="MTPIPGEAPQRLALWSLLFGNFVIGTGVLLPAGLLNVLGADLHV
                     PPATAGLLLFAGGLVVGVGAPVLAGLTSRFDRRVLLAVSAAIFAVGHGLAALAPEFWS
                     LLVIRTVTMVAAALFTPQAAATIGLLVPPQRRSEAIAFIFIGWSLASVVGIPLGSILG
                     TAFGWRATFLVMAVLAAVSCVGVMLSLKPGLRVQPLQFSSWVAVFKNPALLCILLVTL
                     LSMSGQFTVVSYLAPILREAFAATPNNIALMFGIFGVAGVLGNYLATKAIRRFGVDRA
                     ILVALCFLIAGLGMFGLFFGNYVMGCIACFTWGLGTFSSNSLQQSRLVVIAPPLAAAT
                     VALNTSAVYLGQSVGAATGGFAIKDGISASVAWMALGFLAVAIALSMTATRLAARR"
     gene            complement(2860..>3539)
                     /locus_tag="PKV23_03915"
     CDS             complement(2860..>3539)
                     /locus_tag="PKV23_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018453240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HQX84615.1"
                     /translation="AAATTNGKNFMPRLAALLDVMQISDISEVKSADTFVRLIYAGNA
                     VQTVQSTDAKKVITVRTASFAATGETGSAVVEKVTAAADPGLSTYQSENLTKSDRPEL
                     TSAKVIVSGGRGMQNGENFKMLETVARKLGAAVGASRAAVDAGYVPNDYQVGQTGKVV
                     APDLYIAVGISGAIQHLAGMKDSKIIVAINKDDEAPIFQVADFGLVADLFQAVPELDA
                     ELAKILK"
BASE COUNT          629 a          980 c         1125 g          805 t
ORIGIN      
        1 aatgcaaacg agtatgcctt ttagccatga atgagccaaa gcgaaaccgg aattccctga
       61 tctacctcgt cctgcttgcc cttattgcgg ggggcgccgg catttacctg tccggcgatg
      121 cgccgcgcga ggtgccggaa gtcaacggca gcagcggttt caaggctttt gccgcagggt
      181 caatggcggc cttcctggtc aaggcggaca ggccggtcgt cccagacctt gcattcaagg
      241 attcaacggg aagcgggata aagcttaaca gatggcgggg ccgggtggtt ttgctcaatt
      301 tgtgggccac atggtgcgcg ccgtgccgga gggaaatgcc cgccctgaac gcggtgcagc
      361 agcagttggg gtcgaaggat tttgaggttg tggcgttgag cgttgaccgc aaggggctgg
      421 aagcctcggc ggcgttcttg aaggaaacgg gagcagactc actgggcctc tatagcgacg
      481 agacgaccgc ttccctcacg atgctgcagg ccctgggtct gccggtgacc ctgctgattg
      541 accgcaaggg ccgcgaaatc gggcgcctgc tgggaccggc ggaatggaac ggcgcagaag
      601 ccgttgcgat gatcagggcc gccatagccg aacaggatta gcgggtgggg acaggttttt
      661 cgccacgata gtcatagaag ccgcggttcg acttgcgccc cagccagccg gcctcgacat
      721 atttcaccag cagcgggcac ggccggtatt ttgaatcggc caagccttca taaagcacct
      781 gcatcaccga aaggcaggta tccagtccga tgaagtcagc cagttcgagg ggccccatgg
      841 gatgccgggc gccgagcttc atggccgtgt cgatggcttc gacgccgccc acgccctcat
      901 aaagcgtata gatcgcctcg ttgatcatgg ggagcaggat gcggttcacg atgaaagcgg
      961 gaaaatcctc ggccaccgca atggtcttgt ggagggcgag ggtaaattcc ttcacggcgg
     1021 caaaggttgc ctcttccgtg gcaatgccgc ggatgagttc caccagttcc atgacgggca
     1081 cgggattcat gaagtggatg cccatgaatt tctcagggcg gcctgtcact gccgcaagcc
     1141 tggtaatgga aatcgacgag gtgttggagg cgacaatcgt gtgcggcttc aaatgcgggc
     1201 acagggcctg gaaaatcttg cgcttgacct gctcatcctc gacggcggat tcgatgacga
     1261 ggtcgtagtc ggcaaagcct tcgtaagctg caacaggggc aatgcgcttg agagcggcat
     1321 tgcggtcagc ttcggtgatc ttgcctgatt tcagctgccg accgaggttt ccgctgatgg
     1381 tggcaatgcc cgcatcaatg cgctccttgc tgacatcatt gacgccgacg tcaaatccgg
     1441 cgagggcaca gacatgcgca atgccactgc ccatttgccc agcaccaatg atgccgactg
     1501 tcttgatctt catgtccgtt cgtaactcgc ttgatttcag cgtttaagcc tataacgcgc
     1561 acaggtttag agcaagtcta tttcgcaggt gcagcataat cgcacagggc catgcttaac
     1621 tggcgctgga caggtttgga gccggctgct ttcgttttgc tttgtgaaag tttgcttgat
     1681 gacgccgata cccggtgagg ccccgcaacg cttggcgctt tggtccctgc tgtttggcaa
     1741 ttttgtcatc ggtacggggg tattgctccc ggccgggctg ttgaatgtgc tgggagccga
     1801 tctccatgtg ccgccggcca cggcggggct gctgctgttt gccggcggtc tggtggtggg
     1861 cgtgggcgct ccagttctgg cggggctgac gagccggttt gaccggcgcg tgttgctcgc
     1921 ggtgtcggcg gcgattttcg cggtgggcca tggtctggca gcccttgcgc cagaattctg
     1981 gtccctgctt gtcatccgca cggtgaccat ggttgcggcg gcgctgttca caccgcaagc
     2041 tgcggcgaca attggcttgc tggtgccgcc tcaacggcga tcggaagcca ttgccttcat
     2101 ttttatcggc tggtcgctgg cctcggttgt aggcattccg ctgggcagca ttttgggcac
     2161 cgcctttggc tggcgcgcca cgttcctggt catggccgtt ctcgcggccg tgtcttgcgt
     2221 cggcgtcatg ctgtcgctca agccgggctt gcgggttcag cccctgcaat tttcatcgtg
     2281 ggttgcggtt ttcaagaatc ccgcgctctt gtgcatcctg ctggtgacgc ttctcagcat
     2341 gtccgggcag ttcacagtgg tgagctatct cgccccgatt ttgcgcgaag cgtttgccgc
     2401 cacgccaaac aacattgcct tgatgttcgg gatattcggg gttgccgggg ttttgggaaa
     2461 ttatcttgca acaaaggcca tcaggcggtt tggcgtcgac cgggccattc ttgtcgcgct
     2521 gtgtttcctg attgcggggc tcggcatgtt cgggctgttc ttcggcaatt atgtcatggg
     2581 ctgcatcgcc tgtttcactt ggggcctggg aacattttca tccaattcac tgcaacaaag
     2641 caggctggta gtcatcgcgc cgccgcttgc cgcagcaacc gtggcgctca atacctccgc
     2701 cgtctatctt gggcagtcgg taggggccgc caccggcggg tttgcgatca aggacggcat
     2761 ctctgcaagt gtcgcctgga tggctctcgg ttttcttgcc gtggccatcg ccctgtccat
     2821 gaccgcgacg cggcttgcgg cgcgcaggta gagagcctgc tacttcagga ttttggccaa
     2881 ttcggcatca agttccggca cggcctgaaa aagatcggcg acaaggccaa aatccgccac
     2941 ctggaagatg ggagcttcat cgtccttgtt gatggcgaca atgatcttgg aatccttcat
     3001 gccggccaaa tgctgaatgg cccctgaaat gccaacggcg atatagagat caggtgctac
     3061 gaccttgccg gtctggccca cctgataatc attgggaaca tagcccgcat cgacggcggc
     3121 gcgcgaagcg ccaaccgcgg cgccaagctt cctggccacg gtttccagca tcttgaagtt
     3181 ctcgccgttc tgcatgccac ggccaccgga gacgatgacc ttggcggagg tgagttcggg
     3241 gcgatcggac tttgtgagat tttcactctg ataggtcgac aggccgggat cggcggcggc
     3301 ggttactttt tcgaccacgg ctgaaccggt ttctccggtt gcggcaaagc ttgcggtgcg
     3361 cacggtgatg actttcttgg catcggtgga ttgcacggtc tggactgcgt tgccggcgta
     3421 gatcaagcgc acaaaggtgt cggcggattt cacttcggaa atatcggaga tttgcatcac
     3481 atcgagaagt gcggcgaggc gcggcatgaa attcttgccg ttggtcgtag cggcggcca
//
LOCUS       DAORVN010000143         3532 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_22000_length_3532_cov_3.410986, whole genome shotgun sequence.
ACCESSION   DAORVN010000143 DAORVN010000000
VERSION     DAORVN010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3532
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22000_length_3532_cov_3.410986"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(272..1774)
                     /locus_tag="PKV23_03920"
     CDS             complement(272..1774)
                     /locus_tag="PKV23_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008131503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HQX84616.1"
                     /translation="MTVRIGIVRNLMGGAALGLLALGFVGVPLATQPAQAQLEQLPSV
                     ADLAERLLPSVVEISVQSKSEVSGPTLQMPELPDNSPFKDFFDEFLKKQQRGQSPEPQ
                     DRSVSSMGSGFVIDASGLIVTNNHVVEGASVIEVHFQDSTILKAELVGRDPKTDLAVL
                     RVKSELPLPVVTFGDSDTLRIGEWVVAIGNPFGLGGSVSLGIVSARNRDINAGPYDDF
                     IQTDAAINKGNSGGPLFNLKGDVVGINTAIFSPTGGSVGIGFSVPSNTAKGVVDQLVK
                     FGETRRGWFGVKIQSVTDDIAESMAMGKARGALIADVTKTGPAEKAGIEPGDVVIEFN
                     GKAVNAMRDLPRIVADTEIGKKVPIKIFRKGKELQLMAEVGRLEDGEKVVAQQDSSSG
                     ASPAPAVLTALGMTVSSMTDELRQKYKIDADIKGAVVTEVTADSPAADKRLEPGDVIT
                     EAGEKAVTGAADISTSIAEAEKAGKSSVLLLVSKGGKKAEMRFIALKLKK"
     gene            complement(1797..1976)
                     /locus_tag="PKV23_03925"
     CDS             complement(1797..1976)
                     /locus_tag="PKV23_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008547359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HQX84617.1"
                     /translation="MALGLVFVIEGLLYALVPGHLKNMMKAMESVPDETLRLGGLAAL
                     GFGVFVVWMVKAFFV"
     gene            complement(1991..2890)
                     /gene="hflC"
                     /locus_tag="PKV23_03930"
     CDS             complement(1991..2890)
                     /gene="hflC"
                     /locus_tag="PKV23_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008876588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HQX84618.1"
                     /translation="MSSFRNLLGIVLAIIAFIAYSSYFVVDERQKALVLRFGEINRTV
                     EQPGLYFKVPFADTVTYIEDRMLIWENNDRPVQDIASQVYIVNAITLARISDPRLFRE
                     TLGADLVQAEARVAARLDSALRQTYGRRSFDAALSSDRAVMMREIRDELRKEANTFGI
                     DIVDVRVRRTDLSENVLEQTYNRMESERNALAADIRSKGEATKTRMNAETDRTYTTKL
                     ADAQRQSEIIRGEGDGERNKVFAQAFQQDPEFFSFYRSMQAYTKSIGADGTTMVLNPD
                     SEFFKYFGFQTNSGPAAPAAPAQ"
     gene            complement(2911..>3532)
                     /gene="hflK"
                     /locus_tag="PKV23_03935"
     CDS             complement(2911..>3532)
                     /gene="hflK"
                     /locus_tag="PKV23_03935"
                     /inference="COORDINATES: protein motif:HMM:TIGR01933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HQX84619.1"
                     /translation="WRILEPKDFLFNVADQERTIRAVAQSAMREVVGQTRAQMILTTG
                     KDAVSAQVQQITQDLLDEYKAGIIVSQINLGDVQPPAEVANAFAEVVRAGQNQQQLIN
                     EAEKYRNQQVQLAEGEAAKLIEDANAYKARVVFEAQGEAARFLSVYNASKNAKDVTRQ
                     RIYLETMEGILSRSNKVIIEGGQGGSGVVPYLPLLEIQKRATATSQ"
BASE COUNT          692 a         1085 c         1020 g          735 t
ORIGIN      
        1 agctatgccg atccgcggct gtcgctttgc tcgtggagtg aacggacctg gtcccgcgag
       61 gaggtgatcg aggccgccat gcggggcaag atcctgccga tgaaatccac ccgccattgc
      121 ctcactccag aaccgcaaac cacgccaacc gccctgcacg ccctgcgctg aaggctcagc
      181 agggtccatc caagcagcgg tcaggttcaa gagttgggtg gcaagaggaa acttgtctga
      241 cttctcttcc cgcgaagcgg gagaagacga atcacttctt cagcttcagg gcgatgaagc
      301 gcatttcggc tttcttgccg ccttttgaca ccagcaggag caccgaactc ttgccggcct
      361 tttcggcctc ggcaatgctg gtggaaatgt ctgccgcacc ggtgaccgcc ttctcgccgg
      421 cttccgtgat cacgtcgccg ggctccagcc gtttgtcggc ggccgggctg tcagccgtca
      481 cttcggtcac gacggcgccc ttgatgtcgg catctatctt gtatttctgc cggagctcat
      541 cggtcatcga actgactgtc atgccgagtg cggtgagaac cgccggagcg ggcgacgcgc
      601 ctgaactgct gtcctgctgg gccacgacct tttcgccatc ctccagccgg cccacttcag
      661 ccatgagctg aagttccttg cccttgcgga agatcttgat cggcactttc ttgccgattt
      721 cggtgtccgc cacaatgcgc ggcaggtcgc gcatcgcgtt gaccgccttg ccgttgaatt
      781 caatcaccac atcgccaggt tcgatgcccg ccttctcggc agggccggtc ttggtcacat
      841 cggcaatcag cgcgccgcgc gccttgccca tggccatgct ctcggcaatg tcgtcagtca
      901 ccgactggat tttcacgcca aaccagccgc gccgggtctc gccgaattta accagttgat
      961 cgacgacccc cttggccgtg ttggaaggca ccgaaaagcc aatgcccaca gagccgccgg
     1021 tgggcgaaaa gatcgcggtg ttgatgccaa ccacgtcacc cttcaggttg aacagcgggc
     1081 cgccggaatt gcccttgttg atcgccgcat ccgtctggat aaagtcatca tagggcccgg
     1141 cattgatgtc gcggttgcgg gccgagacaa tgccaagcga aaccgaaccg ccaaggccga
     1201 agggatttcc gatggcaacc acccactcgc caatgcgcag cgtgtcgctg tccccgaaag
     1261 tgaccacggg cagcggcaat tctgacttga cgcggagcac cgccagatcg gttttcggat
     1321 cgcgtccgac cagttccgct ttcaggatgg tggaatcctg gaaatgcact tcaatgaccg
     1381 aggcgccctc caccacatga ttgttggtaa cgatcaggcc gcttgcgtca atcacgaagc
     1441 ctgagcccat ggaactcacc gagcggtcct gcggttccgg cgactggccc cgctgctgct
     1501 ttttcaggaa ttcgtcaaag aagtccttga acggcgaatt gtcgggaagc tcgggcatct
     1561 gcagggtggg gccgctgact tccgatttgg actgcactga aatttcgaca accgaaggca
     1621 gcaggcgctc cgccaggtcg gcgaccgatg gaagctgctc caactgggct tgtgccggct
     1681 gcgtggccaa cggcactccg acaaaaccca gggcaagcaa gccgagggcc gcgccaccca
     1741 tgaggttccg cacaatcccg atccgtaccg tcatgtaaat cgcctcccga aagccatcag
     1801 acgaaaaatg cttttaccat ccaaacaacg aaaacgccaa agccgagggc ggccaggccg
     1861 cccaagcgga gcgtctcgtc cggaacgctc tccatggctt tcatcatatt tttcaaatgc
     1921 cccggaacca aggcataaag aaggccctcg atcacgaaaa cgagaccaag ggccatcagc
     1981 agggcttgca tcattgggcg ggtgcggcag gcgcggcagg tccgctgttc gtctggaagc
     2041 caaaatactt gaagaactcg gagtcgggat tcagcaccat ggtggtgcca tcggccccga
     2101 tgctcttggt ataggcctgc atcgaccggt agaagctgaa gaactcgggg tcctgctgga
     2161 acgcctgggc aaacaccttg ttgcgttcgc cgtcgccttc gccccgaatg atttcagact
     2221 ggcgctgggc atcggcaagc ttggtcgtat aggttcggtc cgtttcggca ttcatccggg
     2281 tcttggtcgc ttcgcccttg gagcggatat ccgcggcgag cgcgttgcgc tcggattcca
     2341 tgcggttgta ggtttgctcc agaacgtttt cgctcaggtc ggtgcggcgc acccggacgt
     2401 cgacaatatc gatcccgaag gtattggcct ccttgcgcag ttcatcgcgg atttcacgca
     2461 tcatcacagc ccggtccgat gacagggccg cgtcaaagga gcggcggcca taggtctggc
     2521 gcaaggcgga atccagccgt gccgcgacac gggcctcggc ctgcacgaga tcagcgccaa
     2581 gggtttcacg gaacagccgc gggtcagaga tgcgcgccag ggtaatcgcg ttcacaatat
     2641 agacctggct ggcgatatcc tggaccgggc ggtcattgtt ttcccagatc agcatgcgat
     2701 cctcgatgta ggtcacggtg tcagcgaagg gcaccttgaa atacaggcca ggctgttcaa
     2761 ccgtgcggtt gatttcacca aagcggagga ccagtgcctt ctgccgctca tccactacga
     2821 aataggacga ataggcaatg aaggcgatga tcgccaacac gatgcccaat aggtttctaa
     2881 aactgctcat gtttctatcc taaatctgct ttactggctt gttgcggtgg cgcgcttctg
     2941 gatttccaga agcggcaggt agggcacgac gcccgatccg ccctgcccgc cttcaatgat
     3001 caccttgttg gaccgggaaa gtatcccttc catggtttca agataaatcc gctggcgagt
     3061 gacgtccttg gcgttcttgg acgcattgta aaccgagaga aaccgcgccg cttcgccctg
     3121 tgcttcaaaa accacgcggg ccttgtaggc attggcatct tcgatcagct tggcagcttc
     3181 gccttccgcc agctgaacct gctggttgcg gtatttctcg gcctcgttga tcaactgctg
     3241 ctggttctgc ccggcccgca caacttcagc aaaggcgttt gccacttcgg cgggaggctg
     3301 gacgtcaccg aggttgatct ggctgacaat gatgccggcc ttgtattcat cgagaaggtc
     3361 ttgcgtgatc tgctggacct gggctgaaac ggcgtccttg cccgttgtca gaatcatctg
     3421 cgcccgtgtc tggccaacga cttcgcgcat ggcgctttgt gccaccgccc tgatcgtccg
     3481 ctcctggtcg gccacgttga aaagaaaatc cttcggctca aggatgcgcc ag
//
LOCUS       DAORVN010000144         3531 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_22007_length_3531_cov_3.359321, whole genome shotgun sequence.
ACCESSION   DAORVN010000144 DAORVN010000000
VERSION     DAORVN010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3531
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22007_length_3531_cov_3.359321"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1668)
                     /locus_tag="PKV23_03940"
     CDS             complement(<1..1668)
                     /locus_tag="PKV23_03940"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013420539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HQX84620.1"
                     /translation="MDEAVNAAGRVLEKAKSAVGALLLSDGGLSAAALDLHQHEAHGL
                     AWLATYVDTLKALAACATKLSAQGKLGEIEQLLLKIACGEYLQQVAGGIPMNQTEIFR
                     VENLGIDLKNLRACALYVQGNTRENRAHLVDLMQEQQGELTFGNCGLDDTIDEMRVSM
                     QRFVASKVTPHAHQWHLHNSYVPLEIIQELAELGVFALTLPEGFGGLGMPKEAMCVVS
                     EELSRGWIAVGSLGTRAEIACELILGGGTDEQKAKWLPKITSGEILPTAVFTEPDTGS
                     DLGSLKTRAVKAGDVFKVTGNKIWITHPVRADLMTLLVRSIPEEKGYKGLSMFLAEKP
                     RGSDANPFPAKGMSGSEIAVLGYRGMKEFEIRFEDFEVNAENLLGGIEGQGFKQLMQT
                     FESARIQTAARAIGVAQNALDLGLKYAIDRKQFGKPIIKFPRVADKLALMAAEIMAAR
                     QLCYAAAREKDAGRRCDLEAGMAKLLGARIAWSAADNALQIHGGNGFALEFEISRVLC
                     DARILNIFEGAAVKRHNALVALHMRALVHRHCEVALAQQLARRDFTQL"
     gene            1823..2341
                     /locus_tag="PKV23_03945"
     CDS             1823..2341
                     /locus_tag="PKV23_03945"
                     /inference="COORDINATES: protein motif:HMM:NF018482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion associated locus B family protein"
                     /protein_id="HQX84621.1"
                     /translation="MIPLGRLGGLALLTLTIGMTGSARSAEPVSLGVFTDWTAYTYKA
                     PDTAVCYIVSQPKASDAARKVKRDPIFFIVTHMPGRKINGEVSTIIGYPFKESTMVQL
                     KVDGTEFELFTNGDGAWADTTEKEKKIVTAMKQGQKFTVRGTSWKGTETTDTYSLNGF
                     SAAMDKIDSSCK"
     gene            2349..3086
                     /locus_tag="PKV23_03950"
     CDS             2349..3086
                     /locus_tag="PKV23_03950"
                     /inference="COORDINATES: protein motif:HMM:NF021507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HQX84622.1"
                     /translation="MRRFFPIVLFLCLQSIWAAAGPLCSPLNYEGNAYSVCRIDLRET
                     KLELFNLDGEGAPFRNFTALAETLSQEDKELAFAMNAGMFDDGLKPIGLYIEDGAQAK
                     RLNRRDGYGNFHVKPNGVFYITGDRVGVAATEAYLKLNEKPDFATQSGPMLVIDGKIH
                     PKFSPGGVSYKMRNGVGVLDAHTAIFVISENAVNFYDFALLFRDGLKCANALFFDGTV
                     SSLYSPELGRNDGFAALGPMVGAVKVK"
     gene            3097..>3531
                     /locus_tag="PKV23_03955"
     CDS             3097..>3531
                     /locus_tag="PKV23_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002713993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HQX84623.1"
                     /translation="MTTVLQATTVAPAAYLPNDLRPSLMGLSREELAQILGRVGVPEK
                     QRRMRMRQIWHWIYHRGATSFAEMTDISQSLRVVLDDTFSIGRPDVVTEQKSSDGTRK
                     WLLKLAPDERGQRHEVEMVYIPEEERGTLCISSQVGCTLTCTF"
BASE COUNT          732 a         1002 c         1048 g          749 t
ORIGIN      
        1 gagctgggtg aagtcgcgcc gggccaattg ctgggcaagg gccacctcgc aatgacggtg
       61 gaccagggcg cgcatatgga gcgctaccag ggcattgtgg cgcttgacgg cggcaccctc
      121 gaagatgttc aggatgcggg cgtcgcagag aacgcggctg atttcaaatt ccagagcgaa
      181 gccattgccg ccgtgaatct gcagggcgtt gtcggcagcc gaccaggcga tgcgggcacc
      241 caaaagcttc gccatgcccg cttccaaatc gcacctgcga ccagcgtcct tttctcgcgc
      301 cgccgcatag cagagctggc gcgccgccat gatttcagca gccatcaggg caagcttgtc
      361 ggcgacgcgg gggaacttga tgatgggctt gccgaactgc ttgcggtcaa tggcgtactt
      421 caaaccaaga tcgagtgcat tctgcgccac gccgatggca cgggcggcgg tctggatgcg
      481 ggcactttca aaggtctgca tcagttgctt gaagccctgg ccctcgatcc cgcccagaag
      541 attttcggca ttgacctcaa aatcctcgaa gcggatttca aattccttca tgccgcgata
      601 gcccaggact gcaatttcgc tgccgctcat gccttttgct ggaaatgggt tcgcatcgct
      661 gccccttggc ttctcggcca ggaacatgga tagccccttg tagccctttt cctccggaat
      721 gctccgtaca aggagggtca tcagatcggc gcgcaccgga tgggtgatcc agatcttgtt
      781 gcctgtcacc ttaaagacat cgccggcctt cacggcgcgg gtttttagtg agcccagatc
      841 ggaacccgta tctggttcgg tgaagacggc ggtggggaga atttcaccgc tggttatttt
      901 tggaagccat ttcgctttct gttcatctgt gccgccgccg agaatgagtt cgcaggcgat
      961 ttcggcccgg gtgccgagcg agcccacagc aatccagccg cgcgacagtt cctcggagac
     1021 cacgcacatg gcttccttgg gcatgcccag gccgccaaaa ccttccggaa gggttagggc
     1081 gaagacaccc agttccgcca gttcctggat gatctccaag ggcacgtagg aattatgcaa
     1141 atgccactgg tgcgcatgag gggtgacctt gcttgcgaca aagcgctgca tggagacacg
     1201 catttcgtca atggtgtcat cgagaccgca gttgccgaaa gtcagttcgc cctgctgctc
     1261 ctgcatcaga tccacgaggt gggcgcgatt ctcccgggtg ttgccttgca cataaagggc
     1321 gcaggcgcgc aggtttttga gatcgattcc caaattctca acacggaaaa tctccgtctg
     1381 gttcatggga attccgcctg caacctgctg caggtattca ccgcaggcga ttttcaacag
     1441 gagttgttcg atttcgccaa gcttgccttg ggcggaaagt ttagtggcgc aggcggccag
     1501 ggctttcagg gtgtccacat aggttgccag ccaggccagg ccgtgggctt cgtgctggtg
     1561 caggtcaagg gctgcggcgg aaagcccgcc atccgagagc agcagagcgc caacggccga
     1621 cttggctttt tcaagcaccc gtccagcggc gttcaccgct tcatccatag cttcaatcac
     1681 cagtttcggt ccttgctgcc caaaaattga acgctatccc agctatttgt gcggcgcaac
     1741 atcatgtgcc gcaattgggc cggatttctc cggcaattcg ttgccatgga tttttttatt
     1801 tgttgaggct atgcaggtag acatgattcc actggggcga ttgggcgggc tggccctcct
     1861 gacactgacg attggcatga cgggcagcgc ccgaagtgct gaacccgtct cccttggagt
     1921 ttttaccgat tggacggcct atacctacaa ggccccagac acggcggtct gctacatcgt
     1981 gtcccagccc aaagccagcg atgcggccag aaaagtcaag cgcgacccga ttttcttcat
     2041 cgtcacccat atgccggggc gcaaaatcaa tggcgaggtg tcaaccatca tcggctaccc
     2101 tttcaaggaa tcaaccatgg ttcagctcaa ggttgacggg actgaattcg agcttttcac
     2161 caatggcgac ggggcctggg ccgataccac ggaaaaagag aagaaaatcg tgacggccat
     2221 gaagcagggc cagaagttca cggtacgggg cacctcatgg aagggcaccg aaaccacgga
     2281 tacctattct ttgaatggtt tctccgcagc catggacaag atcgacagct cctgcaaata
     2341 agtaggccat gcggcggttt ttcccaattg tgctgtttct gtgcctgcag tccatttggg
     2401 ctgcggcggg ccccctgtgc tcgcccctga actatgaagg caacgcctat agcgtctgcc
     2461 gcatcgacct ccgggagacc aagctcgaac ttttcaacct cgacggcgaa ggcgcgccat
     2521 tcaggaactt tacggcactg gccgagaccc tgagccagga agacaaggaa ctcgcctttg
     2581 ccatgaatgc aggcatgttt gacgacggac tcaagcccat cggcctttat attgaggacg
     2641 gggcccaagc caagaggctg aaccggcggg atggctacgg caatttccat gtgaagccca
     2701 acggggtatt ttacatcacg ggagaccggg tgggggtggc tgccacggag gcctatttga
     2761 agctcaacga aaagcctgat tttgccacgc aatcggggcc gatgctggtg atcgacggga
     2821 aaatccaccc gaagttttca cctggcgggg tgtcctacaa gatgcgcaac ggggtgggcg
     2881 ttctcgatgc ccatacggcg attttcgtga tctcggaaaa cgcggttaat ttctatgatt
     2941 ttgccctgtt gttcagggac gggctgaaat gcgccaatgc cctgtttttt gacggtacag
     3001 tctccagcct ttacagcccg gaacttggcc ggaatgatgg atttgcggcc ctggggccta
     3061 tggtcggcgc cgtcaaagtg aagtaagacg cgcgccatga ctaccgtttt acaggccacg
     3121 actgtcgcgc cggcggcgta tctgccgaat gatctgcgcc catcgctgat gggcctgtcg
     3181 cgggaagagc ttgcccaaat cctgggccgg gtcggcgttc cggaaaagca gcggcgcatg
     3241 cgcatgcggc agatctggca ctggatctat caccgggggg ccacaagctt tgccgagatg
     3301 acggatattt cccaaagcct gcgggtggtt ctggacgata cgttttccat tggccggccc
     3361 gacgtagtga ccgagcagaa atccagcgac gggacacgga agtggctgct gaaactggcg
     3421 cccgatgaac gcggccagcg ccatgaagtg gaaatggttt atatccctga agaagagcgg
     3481 ggaaccctgt gcatctcaag ccaggtgggc tgcacgctca cctgcacctt t
//
LOCUS       DAORVN010000145         3518 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_22160_length_3518_cov_3.001155, whole genome shotgun sequence.
ACCESSION   DAORVN010000145 DAORVN010000000
VERSION     DAORVN010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3518
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22160_length_3518_cov_3.001155"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..517
                     /locus_tag="PKV23_03960"
     CDS             <1..517
                     /locus_tag="PKV23_03960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX84624.1"
                     /translation="KNFVMGLLTLCSMPSAQAQELGAWIFGQAKAENGAIIHMATLAS
                     STLISSAATDYAALYTISCQTGEPMKWVQQLRLEDALSSRGQVELLATIDQKLPREEF
                     WIVTGNKRTLTRENLPDIAELRTAKTLKLEWNWGWSWLWLSDEAHFELGDAGAIIFTL
                     AKSCGIEEPQW"
     gene            511..1083
                     /locus_tag="PKV23_03965"
     CDS             511..1083
                     /locus_tag="PKV23_03965"
                     /inference="COORDINATES: protein motif:HMM:NF017747.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HutD family protein"
                     /protein_id="HQX84625.1"
                     /translation="MVKILKAEDYREGRWLNGLGLSWDIAATPPDADAQHFEWRMALA
                     RIDSSVAFSYYHNVDRIFMLIEGHGLDLVFDDRELMKVDQPFLPHEFPGDVKTDCVVH
                     NGPCRALNLFIRRGAWKASVRVMGPASNLNIMSAHTTLAFALRGCFTLAEGGTLNSGD
                     ALQLSPGETAQFEPQSEDALLYVAVLTPAR"
     gene            complement(1080..>3518)
                     /gene="dnaE"
                     /locus_tag="PKV23_03970"
     CDS             complement(1080..>3518)
                     /gene="dnaE"
                     /locus_tag="PKV23_03970"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012563101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HQX84626.1"
                     /translation="FELGVIIKMRYPGYFLIVADFIKWAKAQGIPVGPGRGSGAGSLV
                     AYSLTITDLDPLRFQLLFERFLNPDRVSMPDFDIDFCQDRRDEVIDYVQERYGKDHVA
                     QIITFGKLQARAVCRDVGRVLQMPYMQVDRISKLIPMNPANPVTLKQAIDGEPRIQEE
                     RDRDPTVRRMLDIGLKLEGLYRHASTHAAGLVIGDRPLEELVPLYRDPRSSLPATQFN
                     MKYVEKAGLVKFDFLGLKTLTVIDKALKLIHVRKPEFDISKILLDDGQTYHMLAQGDT
                     VGVFQLESSGMKDAVRQMRADRFEDLIALVALYRPGPMANIPTYCARKLGKEPIEYLH
                     PKLEPILKETFGVITYQEQVQQIAKDMAGYTLAEADLLRRAMGKKIKSEMDDQRERFL
                     TGATDQGVDRNIAIQIFEACAKFAEYGFNKSHSAPYAYISYQTAYLKANFPVEFLAAS
                     MTLDMGNTDKLQVFRDEAVRMGIKVAPPSVNASAIDFSVREGKIFYSLAALKNVGVGA
                     IQHLIATREVGGRFISLGDFARRVQGSMLNKRAMESMAKAGAFDEINANRAQVLAGVE
                     TIIATSSRTSAEADAGQNDLFGAATGTQEELVLPLRDVWLPMERLSHEFEAVGFYLSG
                     HPLDDYMKPLAKLGVDSWAAFQQKALTKGATAGKLAGTITHRQERRSKSGNKFAFIGF
                     SDPTGQFEAICFSDTLAVARDLLEPGKAVIARVECDVDGEEIKLRLQGVEILDKAAAA
                     IVTGLTIFVRDTKPLDSIAMRLNSGGKAPVRMILQLESGREVELALGNKFAITPQIKG
                     AIKAIQGVVDVHDF"
BASE COUNT          725 a         1079 c          978 g          736 t
ORIGIN      
        1 caaaaatttt gtaatgggat tgttaaccct gtgctccatg ccgtcggcgc aggcgcaaga
       61 gcttggcgcg tggatttttg gacaagccaa agcggaaaat ggcgcaatca tccatatggc
      121 gaccctggct tcatcgacgc tgatttcatc cgccgcaacc gactatgcgg ctctttacac
      181 aatttcctgc cagaccggag agcccatgaa atgggtccag caattgaggc ttgaggatgc
      241 actttcaagt cgcggccagg tcgagctcct cgccaccatc gaccaaaaat tgccgcgcga
      301 ggaattctgg atagtcactg gcaacaagcg tactctcacc cgtgaaaacc ttcccgatat
      361 tgccgaactt cgcaccgcca agaccctgaa gcttgaatgg aactggggtt ggagctggct
      421 gtggctttcc gacgaagcac attttgaact gggagacgcc ggagcgataa tcttcacgct
      481 cgccaagagt tgcggaattg aagagccgca atggtgaaaa ttttgaaagc tgaagactat
      541 cgggagggcc gctggctgaa tggccttggc ctgtcatggg atattgccgc cacaccgcct
      601 gatgcagacg cccaacactt tgaatggcgc atggccttgg cccggatcga ctccagcgtg
      661 gccttttcct actaccacaa tgtcgatcgc atcttcatgc tgatcgaagg tcatggcctc
      721 gacctggttt tcgatgatcg ggaactcatg aaagtcgatc agccctttct gccccatgaa
      781 tttcccggtg atgtgaagac tgattgtgtg gtgcacaacg ggccttgccg cgcgctgaac
      841 cttttcatcc ggcgaggagc atggaaagct tcggtccgcg tgatgggccc cgccagcaat
      901 cttaacatca tgtccgcaca caccacactc gccttcgcgc tgcggggctg cttcacactt
      961 gctgaaggcg gcacgctcaa cagcggcgat gccttgcaac tttcgccagg tgaaaccgcg
     1021 caattcgaac cgcaaagcga agacgcgttg ctctatgtgg cggtgcttac gcccgctcgc
     1081 tagaaatcat gtacatcaac cacgccctgg atggccttga tcgccccctt gatctgcggc
     1141 gtgatggcga acttgttgcc gagtgccagt tcaacctcgc ggcccgactc caattgcagg
     1201 atcatccgca caggtgcctt gccccctgaa ttgaggcgca tggcgatgga atccagcggc
     1261 ttcgtgtcgc gcacgaaaat cgttagcccg gttacgatcg ccgcagccgc cttgtcaaga
     1321 atctcgacgc cctgcagccg cagcttgatc tcttcgccgt ccacgtcgca ttcaacccgt
     1381 gcaatcaccg ctttgccggg ctccaataaa tcccgcgcca ccgccagcgt atccgaaaag
     1441 cagatggctt cgaactggcc ggtgggatcg gagaatccga tgaaagcgaa tttgttgccg
     1501 gatttcgagc gccgctcctg ccggtgggta atggtgccag ccagctttcc tgcggttgcc
     1561 cccttggtca gggccttctg ctggaaagca gcccagctat ccacaccaag cttggccagc
     1621 ggcttcatat agtcgtcaag cggatgcccc gagaggtaaa agccaaccgc ctcaaactca
     1681 tgggaaagcc gctccatcgg cagccataca tcgcgcaaag gcagcaccag ttcttcctgc
     1741 gtacccgtcg cggcgccgaa caagtcattc tgccccgcgt cggcctccgc actggtgcgg
     1801 ctcgaagtgg caataattgt ttcaaccccc gccaagacct gcgcccggtt ggcattgatt
     1861 tcgtcgaagg ccccggcctt ggccatgctc tccatggccc gcttgttcag catcgagccc
     1921 tgcacgcgcc gtgcaaaatc ccccaatgaa atgaagcgcc cacccacctc gcgcgtcgca
     1981 atgagatgct ggatagcgcc gacgccgaca tttttcagtg ccgccagcga atagaaaatc
     2041 ttgccctcgc gcacgctgaa atcaattgcg ctggcattga ccgatggcgg cgcaactttg
     2101 atccccatgc gcacagcttc atcgcggaac acctgcagct tgtcggtgtt gcccatgtcc
     2161 agcgtcatcg aggcggccag gaattccacc gggaaattgg ccttgagata ggccgtctgg
     2221 taactgatgt aggcataagg cgccgagtgc gatttgttga agccgtattc ggcaaacttg
     2281 gcgcaggcct cgaaaatctg gatggcgatg ttgcggtcga cgccctgatc ggtggcgcca
     2341 gtcaggaaac gttcgcgctg gtcatccatt tccgacttga tcttcttgcc catggcacgg
     2401 cgcagaagat cggcttccgc cagcgtataa cctgccatgt ccttggcaat ctgctgcacc
     2461 tgttcctgat aggtgatgac gccgaaggtt tccttgagaa tgggctcaag cttaggatgg
     2521 aggtattcaa tcggctcctt gcccagcttg cgcgcgcagt aggtgggaat gttcgccatt
     2581 gggcccgggc ggtacaacgc caccagcgcg atcagatcct caaaccggtc ggcccgcatc
     2641 tggcggaccg catccttcat gccggagctt tccaattgga acacccccac cgtgtcaccc
     2701 tgcgccagca tgtggtaggt ctggccgtcg tccagcagga ttttggagat atcaaattcc
     2761 ggcttgcgca catggatgag cttcaaggcc ttgtcgatca cggtgagcgt cttcagtccg
     2821 agaaagtcga atttcaccag cccggctttt tcaacatact tcatgttgaa ttgcgtggcc
     2881 ggaaggctcg agcgtgggtc gcggtaaagc ggcaccagct cttccagggg ccggtcgccg
     2941 atcaccagcc ccgccgcgtg cgtcgaggca tggcggtaaa gcccttcaag cttcaggccg
     3001 atgtccagca tgcgccgcac ggtggggtcg cggtcgcgct cttcctgaat gcgcggctcg
     3061 ccgtcgatgg cctgtttcag cgtcaccgga ttggccgggt tcatcgggat cagttttgaa
     3121 atccggtcca cctgcatgta gggcatctgc aacacccggc ccacgtcacg gcacacggcc
     3181 cgtgcctgca actttccaaa ggtgatgatc tgcgccacat gatccttgcc gtagcgctcc
     3241 tgcacataat cgatcacttc gtcgcgccgg tcctggcaga agtcgatatc gaagtcgggc
     3301 atcgacacgc ggtccggatt gaggaaccgc tcgaacagaa gctggaagcg caacggatca
     3361 agatcggtga tggtgagcga ataggccacc agcgaacccg cccccgagcc gcgccccggc
     3421 cccaccggaa tgccttgcgc cttggcccat ttgatgaaat cggccacgat caggaaatag
     3481 ccgggataac gcatcttgat gatcaccccg agctcgaa
//
LOCUS       DAORVN010000146         3514 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_22195_length_3514_cov_4.248916, whole genome shotgun sequence.
ACCESSION   DAORVN010000146 DAORVN010000000
VERSION     DAORVN010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3514
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22195_length_3514_cov_4.248916"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..559)
                     /locus_tag="PKV23_03975"
     CDS             complement(<1..559)
                     /locus_tag="PKV23_03975"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HQX84627.1"
                     /translation="MFSIIQAAGWPIWPLLFASVIAVALIIERAAALRRSKIVPAGLL
                     QSAVAEYRQSGISEAQLARLEGHSPLGRVLAAGMRNAKSSREIMKEAIEEAGRGVAHE
                     LERFLTTLGTIATIAPLMGLFGTIVGMIEIFGASTAAGNNPQALAHGISVALYNTGFG
                     LIIAIPAMIFYRHFRAQVDGFLVDME"
     gene            982..2328
                     /gene="xseA"
                     /locus_tag="PKV23_03980"
     CDS             982..2328
                     /gene="xseA"
                     /locus_tag="PKV23_03980"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HQX84628.1"
                     /translation="MLVSADVLSITELNRRARATLEREFPLLWVAGEISNLTRAASGH
                     LYFSLKDDAAQARCVMFRSRASSVSWHLENGQQVEAHALVSLYEPRGDFQLNIESLRR
                     AGLGRLFEAFARLKERLETEGLFADARKRQLPRFPRRVGIITSPGAAALRDVLVALKR
                     RAPHLQLVLYPALVQGAAAAAEIAAAIATASRRNECDLLLVVRGGGSIEDLWAYNEEV
                     VARAIAASNLPVISGIGHETDTTIADLAADLRAATPTAAAELATQGWHEAAAKISELG
                     TALGRAARYRVARDQQRVDHLSLRLVHPATRLGQARDRLALLGSQLDASLARNLRQHR
                     DRLNRAALGLSRTMPRTEKHRGGIELLVQRLAAAIREQHGKRVATLDNIAGALSHLNP
                     DATLARGFSVVRDAHGKLVTDASRLHAGQRVDIYLAHGSAEAGILTCSPDHGPVAT"
     gene            2392..2976
                     /locus_tag="PKV23_03985"
     CDS             2392..2976
                     /locus_tag="PKV23_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HQX84629.1"
                     /translation="MEHTLPPLPYAMDALVPHISKETFEYHYGKHHQAYATNLNNLIK
                     GTEYESLDLEAIIKKAPAGGIFNNSAQVWNHSFFWNSMKVGGGGTPSGSLADAINKKW
                     GSFDEFKKAFQASAVGNFGSGWTWLVKKPDGSVDIVNTSGAGTPLNTDNKALLTIDVW
                     EHAYYIDYRNARPKFVETFLASLANWDFAASNFG"
     gene            3063..3263
                     /locus_tag="PKV23_03990"
     CDS             3063..3263
                     /locus_tag="PKV23_03990"
                     /inference="COORDINATES: protein motif:HMM:NF003316.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SlyX family protein"
                     /protein_id="HQX84630.1"
                     /translation="MEARITELEIKLGLTEDHLEELNRTVFRQQQQIDLLQAQIRELY
                     LLMQPAGPGEARNPRDEIPPHY"
     gene            complement(3273..>3514)
                     /locus_tag="PKV23_03995"
     CDS             complement(3273..>3514)
                     /locus_tag="PKV23_03995"
                     /inference="COORDINATES: protein motif:HMM:NF014678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HQX84631.1"
                     /translation="EVAKHCMWMRNTLVPLSVAFLDDKGRILNVEDMQPKTEVNHCSV
                     SPVRYALEMNLGWFRSRGLGPGFAITGVDKVPAGR"
BASE COUNT          755 a         1084 c         1005 g          670 t
ORIGIN      
        1 gctccatgtc gaccagaaaa ccatccacct gggcccggaa atgtcggtag aagatcatgg
       61 ccggaatagc gatgatcagg ccgaaaccgg tgttgtacag cgccaccgaa atgccatgtg
      121 cgagtgcctg cggattgttg cccgcggcag tcgatgcacc gaagatttcg atcatgccga
      181 cgatcgtgcc aaacaggccc atcaacggtg cgatcgtggc gatggtgccc aacgtcgtga
      241 ggaatcgttc cagttcatgg gccaccccgc gccccgcttc ttcgatcgcc tccttcatga
      301 tttcgcgaga actctttgcg ttgcgcatgc cggccgcaag tacccgcccc agcggcgaat
      361 ggccctcaag gcgcgccagt tgagcctccg agattccgct ttgacggtat tcggcaaccg
      421 cgctttgcag cagccccgcg ggaacgatct tgctgcggcg cagtgccgcg gcgcgttcaa
      481 tgatgagcgc gactgcgatg acggaagcga acagcaacgg ccagataggc cagccagcag
      541 cctggatgat ggaaaacacg tgggtgactc cggaaagcgg gaaagcgcga actttagccc
      601 gagtgggaag attcagcaat aaaaaatggg gttgccgatc aggcaagggt gttgcaaacc
      661 ggttttcgcc gggcagaaga atgaatcgac aggctgtgcg gccgtcgctt cgtcatcagc
      721 cacggaagcc ttcggcaaat tgtccacaaa atctgtggat aagattgtgg atcatcgtct
      781 ggtcccgggg ctgaagcagc gttgcgtctg ggcttggcat tttttgcgca aattttggcc
      841 agaaataatt ctcgaccatt cagatggtta cacgaaaaca aagaccttga atggggctcc
      901 tcctgcccgc gaaaccggcc ccacgcgggg atgctgtagt atttctttcc tcccccatcc
      961 tttcccgacc acgttccgtc catgctcgtt tcagctgacg tcctcagcat caccgaactc
     1021 aatcgccggg cgcgcgcaac cctggaacga gagttccccc tgctttgggt ggccggtgaa
     1081 atttccaacc tgacccgcgc tgcgtcaggc cacctctatt tttcgctgaa ggacgatgca
     1141 gctcaggccc gctgtgtcat gtttcgctca cgcgccagtt cggtttcctg gcacctggaa
     1201 aacgggcagc aggtcgaggc ccatgcgtta gtttcgctct atgaaccccg cggcgacttc
     1261 cagctcaaca tagagtcctt gcgccgcgcc ggattgggac gtctgttcga agcctttgcc
     1321 cgattgaagg agcgactgga gaccgaaggc ctgtttgccg acgcgcgcaa acgccagctg
     1381 ccccgctttc cgcgccgggt cggaatcatc acttcgccgg gtgctgccgc cctgcgcgat
     1441 gtactggtgg ccctgaagcg ccgtgcgccg cacctgcagc ttgttcttta tcctgcgctg
     1501 gtgcagggcg cagcagccgc agccgaaatt gctgcggcaa tcgccacggc gtccaggcgc
     1561 aatgaatgcg acctgctgct tgtcgtacgc ggcggtggca gcatcgagga tctttgggca
     1621 tacaacgaag aggtcgtcgc acgagccatc gccgcaagca acctgcccgt catcagcggc
     1681 atcgggcatg agacggatac caccatcgcc gaccttgccg ccgacttgcg cgcggcgacg
     1741 ccaactgccg cggcagagct tgccactcag ggatggcacg aagccgccgc caaaatttcc
     1801 gaacttggaa cagcgctggg cagggccgcc aggtatcgcg tcgcccgcga ccagcagcgg
     1861 gtggatcacc tctccctgcg ccttgtccat ccggcaacgc gattgggcca ggcacgggat
     1921 agattagcct tgctgggatc gcaacttgat gcctccctgg cccgcaacct gcgccagcat
     1981 cgggaccggc tgaatcgcgc ggctctgggt ctgagccgaa ccatgccacg caccgaaaaa
     2041 caccgggggg gtatcgaact gttggtgcaa cgactagctg ccgcaatccg tgaacaacac
     2101 gggaagcgcg tggccacgct ggacaatatt gcgggggcgc tctcccatct gaaccccgat
     2161 gcaaccctgg ccaggggctt ttccgttgtt cgtgacgccc acggaaaact cgtaacggat
     2221 gcctcccgac tgcatgccgg ccaacgcgtg gacatctatt tggcacacgg cagtgccgaa
     2281 gccggcatcc tgacctgcag cccggatcat ggtccagttg ccacctgaac tattcttgac
     2341 tataatagtc aactttaaac ttcccaccct cactgcaaca ggagaacctc catggaacac
     2401 acactccccc ccctgccgta tgcgatggat gctctggtcc cgcacatttc gaaggaaacc
     2461 tttgaatacc actatggcaa gcatcatcag gcctacgcaa ccaacctcaa caatctgatc
     2521 aagggcaccg aatacgagag cctggacctg gaagccatca tcaagaaagc accggccggc
     2581 ggcatattca acaattcggc ccaggtctgg aatcacagct ttttctggaa cagcatgaaa
     2641 gtcggcggcg gcggcacgcc gagcggctcc ctggcggatg cgatcaacaa gaaatggggc
     2701 agcttcgacg agttcaaaaa ggccttccag gcctccgctg tcggcaactt cggctctggc
     2761 tggacctggc tggtgaaaaa gccggatggc tcggtggata tcgtcaacac ctcgggcgca
     2821 ggcacgccgt tgaacaccga caacaaggct ctactgacca tcgacgtctg ggaacacgcc
     2881 tattacatcg actaccgcaa tgcgcggccc aagtttgtcg aaaccttcct ggcttcattg
     2941 gccaactggg attttgccgc aagcaatttc ggctgataga ggcaggtaag tcaaagcaac
     3001 caagacagca aagggcgccg tgaggcgccc tttgtctttg tcattggatg cggggggcag
     3061 aaatggaagc cagaatcacg gagctcgaaa tcaagctggg cttgaccgag gatcatctcg
     3121 aagaactcaa tcgcacggtg tttcgccagc agcagcaaat cgacctgctg caggcccaga
     3181 ttcgagagtt gtacctgttg atgcaaccgg caggcccggg tgaagcgcgc aatccgcgcg
     3241 atgaaatccc gccgcactat tgaaaccggg tatcagcgtc ctgcggggac cttgtcgacc
     3301 ccagtgatgg caaatcccgg ccccaggccg cggctcctga accagcccag gttcatttcc
     3361 agggcataac gcaccggact gacagagcaa tgattgacct cggtcttggg ttgcatgtcc
     3421 tcgacattga gtatccggcc tttgtcatca aggaaggcca ctgacaaggg caccagggta
     3481 ttgcgcatcc acatgcagtg cttcgccacc tcgg
//
LOCUS       DAORVN010000147         3496 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_22407_length_3496_cov_3.229294, whole genome shotgun sequence.
ACCESSION   DAORVN010000147 DAORVN010000000
VERSION     DAORVN010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3496)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3496)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3496
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22407_length_3496_cov_3.229294"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1017)
                     /locus_tag="PKV23_04000"
     CDS             complement(<1..1017)
                     /locus_tag="PKV23_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006468574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQX84632.1"
                     /translation="MGKTTAGERETVLRLSGITKQFGALLANDDISLSLRRGEILGLL
                     GENGAGKTTLMNILFGHYVADAGEIEVEGKVLPPGLPAAALAAGIGMVHQHFTLAENL
                     TVLDNIVLGTESLWQLSSNSQKARTRIRQLAQEFGLEVDPDAIVASLSVGERQRVEIL
                     KALYRKAKILVLDEPTAVLTPQESAALFETLKRFVAKGLSIIFISHKLGEILAACSRV
                     SVLRGGRLVAERGVAGVTRSELAELMLGRSIPQAKAEIQKSGEVVLGLHGIHARHSSQ
                     VLDGITLDIRAGEILGIAGVSGNGQSLLADVVSGVAVPTAGTLALKGATLSRTGPRVM
                     VNLGV"
     gene            complement(1023..2045)
                     /locus_tag="PKV23_04005"
     CDS             complement(1023..2045)
                     /locus_tag="PKV23_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008128205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HQX84633.1"
                     /translation="MTNVFRVNKRDVLKFTAAGALLPLAGMFPRLANAAGPIKTAAIY
                     TVPVEQQWVSRIHKAATIAKDRGDIEYVFSEKVSNTDYERVMREYCEAGNQLIVGEAF
                     AVEDAARAVAKDYPDRAFLIGSSFKPDAAAAPNFAVFDNYIQDASYLSGIVAGAMTKT
                     GKIGMVGGYPIPEVNRLMNAFMAGVRETRADATFLVAFIGSWFDPPKAKETALAQIDA
                     GADLLYAERFGVSDAAKEKGILAVGNVIDTQADYPDTVVASALWHFEPTLDAAIAALK
                     AGAFKADDYGQYSFMKAGGSSLAPYGTFESKIPDDVKAKVAAKEADIKSGAFSVAIDD
                     NEPKSS"
     gene            complement(2261..3031)
                     /locus_tag="PKV23_04010"
     CDS             complement(2261..3031)
                     /locus_tag="PKV23_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010915720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQX84634.1"
                     /translation="MTGRLHHRSALVIGGARGIGAAIAERFFEEGAWVVIADTEEEAG
                     RATAERLKGRFISTDISQKIDAERAVSEAVTAYGGLDIIVQNAGIYPWTLIENIEPEE
                     WDQVMAVNLKGTYLAARAALPVMKARKQGRMIFTSSITGPRVSSPGHGHYSASKAGIN
                     GFIKAAALEFSSFGITVNGIEPGNILTEGMKLHRSAGFIKTMKDAVPLGRLGTPRDIA
                     NAALFLASDDASYITGTTIIVDGGQTLPEGKDFMINPE"
     gene            complement(3028..>3496)
                     /locus_tag="PKV23_04015"
     CDS             complement(3028..>3496)
                     /locus_tag="PKV23_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019857727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQX84635.1"
                     /translation="LIAWIAIACFALFTFIMAKTPFGRHITMIGGNAVATFRAGIKVD
                     KLVVFSFVLAGAIAGVAGWLLAIRTSGATANLGTGMLFNAFAAVVIGGVSLKGGVGRL
                     PGVYAGVLLLSSIHTAINLMGLPAHFTQMILGGLVLVAVLLDTVKLSIRQKLA"
BASE COUNT          733 a         1009 c         1040 g          714 t
ORIGIN      
        1 gaccccgaga ttgaccatca cgcgcgggcc tgtccgggaa agggttgctc ccttcagcgc
       61 cagggttccg gctgttggca cagcaacgcc actcaccaca tcggcgagca ggctttggcc
      121 attgcccgag actccggcta ttcccaaaat ctcgccagca cggatatcaa gcgtgatgcc
      181 gtcaaggacc tgggaactgt gccgggcatg aattccgtgc aagcccagga caacttcccc
      241 tgacttctga atttcggcct ttgcctgcgg aatgctgcgg ccaagcatca actcggcaag
      301 ttcagatcgt gtgacccctg caacgccgcg ttccgccacc agcctgccgc cgcgcaggac
      361 gctgacacgg ctgcaggcgg cgaggatttc gccaagctta tgggaaatga aaataataga
      421 gaggcccttg gccacaaagc gcttcagggt ttcaaacagg gcggctgatt cctggggcgt
      481 cagaaccgcg gtgggttcat ccagaaccag gattttggct ttgcggtaaa gcgccttcag
      541 aatttcaaca cgctggcgct cgcccaccga tagcgatgca acaattgcat ccgggtccac
      601 ctccaagcca aactcctggg ccagctgccg tatgcgcgtg cgggcctttt gagaattgga
      661 ggagagctgc cacagggatt ccgttccgag cacaatattg tcgagcaccg tcaggttttc
      721 agcaagggtg aaatgctggt gcaccatgcc aatgccggcg gcaagggcgg cggcaggcag
      781 gccgggcggc agcaccttgc cttcaacctc gatctctccc gcatctgcca cataatgccc
      841 gaacaggatg ttcatgagcg tggttttgcc ggcgccattt tcacccagca gccccaagat
      901 ctcgccgcgc cgcagggaca gtgaaatgtc atcattggcc agcaatgcgc caaactgttt
      961 ggtgatgccg gaaagccgga gaacggtttc acgctctccg gctgttgtct tgcccatgcc
     1021 cgtcaggagg atttgggttc gttgtcgtca atggcaaccg agaaggcgcc cgatttgata
     1081 tcagcttcct tggcggcaac cttggctttc acgtcatcgg gaattttcga ctcgaaagtt
     1141 ccgtatgggg caaggctcga gccgcctgcc ttcatgaagg aatattggcc gtagtcatcg
     1201 gccttgaagg cgccagcctt gagcgcggca atggcagcat ccagggtggg ctcaaaatgc
     1261 caaagggctg aggccacaac cgtgtccgga tagtcggctt gcgtgtcgat cacgttgcca
     1321 accgccagaa tgcctttttc ctttgcggca tccgaaacgc caaaccgttc cgcatagaga
     1381 aggtcggcac ctgcgtcgat ttgggcaagg gctgtttcct tggcctttgg cggatcaaac
     1441 caggagccga tgaaggccac caggaacgtg gcatcagcgc gggtttcgcg cacccctgcc
     1501 ataaacgcat tcatcaggcg gttcacttcc gggatgggat agccgccaac catgccgatt
     1561 ttgccggtct tggtcatggc gcccgcgacg atcccgctga gataggacgc atcctggatg
     1621 taattgtcga acaccgcgaa atttggcgcc gccgctgcat cgggtttgaa gcttgagccg
     1681 atgaggaagg cacggtccgg atagtccttg gcaacggcgc gcgccgcatc ctcaacggca
     1741 aaggcctcgc caacgatgag ctggtttcca gcttcgcaat attcgcgcat gacgcgctca
     1801 taatccgtgt tggaaacttt ttcggagaag acgtattcaa tgtcgccacg gtctttggcg
     1861 atggttgcgg ccttgtgaat gcggctcacc cactgctgtt cgaccggcac tgtataaatt
     1921 gctgctgttt tgatggggcc tgcagcattc gcaagccggg gaaacatgcc ggccagaggc
     1981 aatagggctc cggcggcggt gaatttcagg acgtcgcgtt tgttcacgcg gaaaacattg
     2041 gtcatggttc gactccctcc aagggcctta tgccccttta acgggatact acacttgcgg
     2101 aaattcttgt ccagaaggtc aaattaggaa gtgaactcat aaacgggcag ggtcagatca
     2161 tgcacctttg tctgcttccg aggcgattgc ggccatagtc ggcaaccaat gccatggcgc
     2221 ctctgggcaa gaagcagcat tggtcgggca cactcagcgg ctattccggg ttgatcatga
     2281 agtcctttcc ttccggcagg gtttgcccgc cgtcgacgat gattgtcgtg ccagtaatgt
     2341 atgacgcatc gtcggaggca agaaacaagg ccgcattggc aatatcgcgg ggcgttccaa
     2401 ggcggccaag cggaacggcg tctttcatgg ttttgataaa gccggccgag cgatgcagct
     2461 tcatcccttc ggtgagaata ttgccgggct cgatgccatt caccgtgatg ccaaagctgg
     2521 aaaactcaag ggccgccgcc ttgatgaagc cattgatgcc ggccttggaa gccgaataat
     2581 ggccatgccc ggggctgctc acgcgtgggc ccgtgatcga tgacgtgaaa atcatccggc
     2641 cctgtttgcg tgctttcatg acgggcagtg cggcccgtgc cgcaagatag gtgcccttta
     2701 ggttgacggc catcacctgg tcccattctt cgggttcaat gttttcaatc agtgtccaag
     2761 gataaatgcc ggcattctgc acaatgatat cgagtccgcc ataggctgta acggcctcac
     2821 tcaccgcccg ctccgcgtcg attttctgcg aaatgtcagt ggagatgaag cggcccttga
     2881 ggcgttcagc ggtggctcgg cccgcttcct cttcggtgtc tgcgatcacg acccacgccc
     2941 cttcctcgaa aaagcgctcg gcaattgccg caccaatgcc gcgggcgccg ccgatcacca
     3001 gggcgctgcg atgatggagg cggccggtca tgccagtttc tgccggattg aaagtttcac
     3061 cgtgtcgaga aggacagcga caagcaccag tcctccgagg atcatctggg tgaaatgggc
     3121 aggaagcccc atgagattta tcgcggtgtg aatggacgag agcagcaaca caccggcata
     3181 tacgccaggc aaccggccga cgccgccttt caggctgacg ccgcctatca ccacggcggc
     3241 aaaggcattg aacagcatgc cggtgcccaa attggcggtg gcgccggagg tgcggatggc
     3301 cagcagccag ccggcaacgc ctgctatggc gccggccagc acgaaagaaa ataccacaag
     3361 cttatccacc ttgatgccgg cgcggaaggt ggcaacggca ttgccgccga tcatggtgat
     3421 gtggcggcca aagggcgtct tggccatgat gaaggtgaag agggcgaaac aggcgatggc
     3481 gatccaggcg atgagc
//
LOCUS       DAORVN010000148         3488 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_22482_length_3488_cov_4.484707, whole genome shotgun sequence.
ACCESSION   DAORVN010000148 DAORVN010000000
VERSION     DAORVN010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3488
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22482_length_3488_cov_4.484707"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="PKV23_04020"
     CDS             <1..320
                     /locus_tag="PKV23_04020"
                     /inference="COORDINATES: protein motif:HMM:NF013362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQX84636.1"
                     /translation="LKSPQQLAGLVILQQKMLTHAARLVRPGGKLVYCTCSLSPLEGE
                     RQVFNFLRSRDDFRLSPIIPDELAQQVQFITPAGLMRCLPSMVIGKGSGLDGFFAARL
                     ERR"
     gene            397..2052
                     /locus_tag="PKV23_04025"
     CDS             397..2052
                     /locus_tag="PKV23_04025"
                     /inference="COORDINATES: protein motif:HMM:NF019552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HQX84637.1"
                     /translation="MRDHWATQQFATPTYFQVLSDAALKLWASHGLGLGTTQHISICG
                     LGTKLLTLKPGDPIQAQVMARGKFSFAGVTVSVKPIDIFDARPPSPEWATALHNLSWL
                     QHFVTGGHELHRIVARSLVLRWGEQRNRLRTSLANFQALIALASAAHFLVGPLPSSFG
                     KPFYALAQKLTHRVMATRPSSVSDSFWQAVALQYASLAFRSSETLRELANARMCDVIN
                     DVILPDGGHVSRNPQALLEVLLNVIPIREAMLAKREAVPQALSVAIERMFPMLRMLSH
                     GDRGLSNFHGAGVTDMTSIRTILEHDIIAGRPLLSAPHSGYCRISHRSGLLIMDTGAP
                     MECVSPLAIEFSDGPHRIFSNCGMPASASPAWRRAAADIAAHNTTEVMSFAGGPQNAL
                     VAEVISSPQGSLIRSVNEIAGNTGKVSHHRSIFLSQTGTDLRGEDQISPGESNGSYPG
                     PFDFTIRFHLHPGVKASFNRKGTQIVIVLANRTAWQFMARGGSLTLEESVFLGDPSGP
                     RKTQQIVIRGSTDTAAPVNWALRRVEKSATAANDPAEMPQLPF"
     gene            2113..>3488
                     /gene="purH"
                     /locus_tag="PKV23_04030"
     CDS             2113..>3488
                     /gene="purH"
                     /locus_tag="PKV23_04030"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014438890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HQX84638.1"
                     /translation="MTHPIRRALISVSDKTGLIEFARALASLSIEIISTGGTAKSLQA
                     AGIPAIDAASVTGFPEIMDGRVKTLHPKIHGGLLAIRNDAAHESAKKSHGIADIDLLV
                     VNLYPFEETVAKQADWSTTIENIDVGGPAMIRAAAKNHARVTVIVGPEDYATVLSELK
                     SHSGNTTEATRRSLAAKAFARTAAYDAAIAQWFSKELGDATPDYFAIGGKRLQNLRYG
                     ENPHQSAALYDSPDQRKGVASAKQVQGKELSYNNINDTDAALDCLSEFRNEPEAACVI
                     VKHANPCGVAIGHSLFEAYGKALACDPVSAFGGILAFNRNLDEETAFEISKLFTEVII
                     APGADEGARGIMAAKKNLRLLLLGELPNSNAPAISVKSIIGGLLVQSRDGYSVDEMNL
                     RVVTQREPTISEMKDLKFAFRIAKHVKSNAIIYAKNGATVGIGAGQMSRVDAARIAVR
                     KSEDAARAA"
BASE COUNT          750 a         1065 c          930 g          743 t
ORIGIN      
        1 atttgaaatc cccccagcag cttgcagggc tggtgatcct gcagcagaaa atgctcaccc
       61 atgccgcacg tctggtaagg ccgggcggga agctggtcta ttgcacctgt tccctgtctc
      121 ccctcgaggg cgaacgccag gtgttcaatt tcctccggtc ccgcgacgac ttcaggcttt
      181 cgcccatcat tccagatgaa cttgcacagc aggtacaatt cataacccct gcgggcctta
      241 tgcgctgcct gccgtccatg gtgatcggca aggggtccgg cctcgacggc ttctttgccg
      301 caaggctcga gcggcgttag taattcgtta accatctcgg caccagtgtg ggcctttgca
      361 ccatcaccgg aacaacgggt ttcaacgcgg cagaacatgc gcgatcattg ggctacgcag
      421 caattcgcca ctcccaccta ttttcaggtg ctgtcggacg ctgcgctcaa gctctgggct
      481 tcgcatggac ttggccttgg cacaacccag cacataagca tttgcggtct gggcacaaag
      541 ttattgacgc tgaaacctgg ggatccaatc caggctcaag taatggcccg tggcaagttc
      601 agcttcgccg gagtaaccgt aagcgtaaaa ccgatcgaca tatttgacgc caggccgccc
      661 agtcctgaat gggccacagc cctccacaat ctgtcctggc tgcagcactt cgtaaccggc
      721 ggccatgagc tgcatcgcat cgtcgcccgc tcgctggtgc tgaggtgggg cgagcaacgg
      781 aacagactcc gcacgtccct ggcaaatttt caggcactca tcgcgctcgc aagtgccgcg
      841 cattttcttg tgggccccct gccctcttca tttggaaagc cattctacgc ccttgcccaa
      901 aagctcaccc atcgtgtgat ggcaacgcgg ccctcatcag tcagtgacag tttctggcag
      961 gcggttgctc tgcaatatgc aagccttgcc tttcgcagtt cagagacgct gcgtgaactg
     1021 gccaatgcca ggatgtgcga tgttatcaac gatgtcattc ttcctgatgg cggacacgtc
     1081 tcgcgcaacc ctcaggcttt gctcgaggtg ctccttaacg tcatcccaat cagggaagca
     1141 atgctggcaa agcgtgaggc tgtcccgcag gcattgagcg ttgccattga gcggatgttc
     1201 cccatgctcc ggatgctcag tcatggcgac cggggcctca gcaattttca cggcgccggc
     1261 gtcactgata tgaccagcat caggactatt ttggaacatg acataattgc tggtcggccg
     1321 ctgctctcgg cacctcattc cggttattgc aggatttcgc accggtccgg cctcctgatc
     1381 atggacaccg gcgcaccaat ggaatgtgtg agcccgctgg cgatcgagtt ttccgatggt
     1441 ccccatcgca ttttcagtaa ttgcggcatg ccggcctccg cttcgccggc ctggcgtagg
     1501 gctgccgcag atatcgcggc ccacaatacg actgaagtca tgtccttcgc tggcggaccg
     1561 caaaatgctc tggttgccga agtgatcagt tcgccccagg gctcgctgat ccgttccgtc
     1621 aatgaaattg caggcaacac cggcaaggtc agtcaccacc ggagcatctt tctctcgcaa
     1681 accggtactg acctccgggg tgaggatcag atttcacccg gcgaatccaa tggctcgtac
     1741 cccggtccgt tcgatttcac catcaggttt catctccatc ccggcgtgaa ggcaagcttc
     1801 aaccgcaagg gaacgcagat tgtcattgtg ctggccaacc gaaccgcatg gcaattcatg
     1861 gcccggggcg ggagcttgac actggaagaa agtgtgtttc tgggagatcc gtctgggccc
     1921 cggaaaaccc agcaaattgt catccggggt tcgacggaca ccgcggcacc ggtaaactgg
     1981 gccctgcgcc gcgtcgagaa atcggccacg gctgccaacg accccgcgga aatgccgcag
     2041 cttcctttct agtggcctaa tcattccggc gtgctatgtc gctgatgagt ttttagttcc
     2101 tggaaattac ccatgaccca tccgatccgc cgcgccctca tttccgtttc cgataagacc
     2161 ggcctgattg aatttgcccg cgcccttgca tccctcagca tcgagatcat ttctaccgga
     2221 ggcaccgcga agagcctgca agccgcaggc attccggcca ttgatgcggc ctcggtgact
     2281 ggctttcctg agattatgga cggccgggtg aagaccctgc atccgaaaat ccatggcggt
     2341 cttttggcaa ttagaaacga tgcggcccac gaaagcgcca agaaaagtca tggcatcgct
     2401 gacattgatc ttctggtggt taatctttat ccgtttgaag agactgtcgc caagcaggcc
     2461 gactggagca ccaccataga aaacatcgat gtcggcggac ctgccatgat ccgggccgct
     2521 gccaagaatc atgctcgggt gacagtcatt gtcggtcccg aagactatgc aactgtgctg
     2581 tccgaactca aatcccatag tggaaacacg accgaggcaa cgcggcgttc tctcgcggcc
     2641 aaggccttcg ccagaaccgc tgcctatgac gccgcaatcg ctcaatggtt ttcaaaagaa
     2701 ctcggcgacg ccacgccgga ttattttgcc attggcggca agcgcctgca aaacctgcgc
     2761 tacggcgaaa acccccacca gagcgccgcc ctctatgaca gcccggatca acgaaaaggt
     2821 gtagcaagcg caaaacaggt tcaaggcaag gaactgtcct ataacaacat caatgacacg
     2881 gacgcagccc tcgattgtct ctcggaattc cgcaatgagc cggaagccgc ctgcgttatc
     2941 gtaaaacatg ccaacccttg cggcgttgcc atcggccatt cgctatttga agcctatggc
     3001 aaggccctgg cctgcgatcc ggtcagcgcc tttggcggca tcctggcgtt taaccggaat
     3061 ctggatgaag aaacagcttt cgaaatttca aaacttttca ccgaagtcat tattgctccg
     3121 ggcgcggacg aaggcgcacg gggcatcatg gcggccaaaa aaaatcttcg cctcctgctt
     3181 ctgggtgaac ttcccaattc aaacgccccg gcaatttccg tgaagtccat catcggcgga
     3241 ctcctcgtgc aaagccgcga cggctattcc gttgacgaga tgaacttgcg ggtcgtaacc
     3301 cagcgtgaac ctaccatttc cgaaatgaag gatctcaagt ttgccttccg gattgccaag
     3361 cacgtaaaat ccaacgcaat catttacgcc aagaatggcg caactgtcgg catcggcgcc
     3421 gggcaaatgt cacgcgtcga tgccgcccgc atcgcggttc gcaaaagcga agatgcggcg
     3481 agggccgc
//
LOCUS       DAORVN010000149         3481 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_22564_length_3481_cov_2.707823, whole genome shotgun sequence.
ACCESSION   DAORVN010000149 DAORVN010000000
VERSION     DAORVN010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3481
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22564_length_3481_cov_2.707823"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1766
                     /locus_tag="PKV23_04035"
     CDS             <1..1766
                     /locus_tag="PKV23_04035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX84639.1"
                     /translation="AIANLKIKQGKTGFSISYDATCSDAEQSLKYQAKIEASADGRLA
                     FSAVGTPLSDFLTNRSGFVILHPLEGVVGKPVEVVHVDGSREKRNFPKIISPGQPIFE
                     IRSLKHTAMPGVTATVLMEGNKFEMEDHRNWMDASYKTYVCSLLDPWPYTLKKGEAFT
                     QSVTLTISGKPTAKASSKSGGAISVAMGKAKGRMPSIGVGVPMAEAQHAVAKADLIAA
                     AKPGQLVFQIDGRKENQTEAAAAFRALKERTGIPATLEIVLPAKAPAMEEVEAIAGAL
                     RAAGFKPDAVVITQMHDLKSFQPNTPRPWGPTYEEMAAAARKAFPGVTLGGGMLSYFT
                     ELNRKPAPKGVFDFITHTACPIVHAADDLSVMETLESMPSIIASTRAMIGTSPYHMGP
                     TSIPCRDNPYGAREASNPDNGRVCLSDMDPRQRGLFGAAWNLGLVAAAAKGKLDAVSL
                     GAVTGPQGLIYRMANYMQPGLDGGNAAVYPVYHILAGLGRASGARHIETESGAPGKIA
                     ALAHESKAGPVLWLANLTGEKQAVKVGGFKGAAQVHVLDEKTFDGLIKRPDYLAKPGT
                     KMNKVSSLELGPYAVIRVAAT"
     gene            1770..3029
                     /locus_tag="PKV23_04040"
     CDS             1770..3029
                     /locus_tag="PKV23_04040"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018185460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HQX84640.1"
                     /translation="MLLGCIADDFTGATDLANTLSRHGMRTVQIIGEPTGLPPDTDAI
                     VVALKSRTIPAKDAVAQSLAACRWLKQHGARQIYFKYCSTFDSTDAGNIGPVADALLD
                     ELGSSFTIACPAFPENGRTIYMGHLFVGQVLLSDSSMRHHPLTPMHDSNLVNVLARQT
                     PHRTGLVAHATVKQGAGAIAAAFAELQSRGIRHAIIDAIEDIDLHYLGEAVANLDVVT
                     GGSGAAIGLPENFRKAGLLSKRENVKPLAPVGGGGVVLSGSSSAATLAQVAAMSAKHP
                     AFAIDVAALFSNKDKAIYEVLLWAVEKLKASPALIYASAPPEKVSEIQSRFGREKAGA
                     VIEEAIAAIATGLAKSGVRRFVVAGGETSGAVVSALGVKVLEIGPQISPGVPATLSYG
                     EPRYALALKSGNFGGPEFFSEALEALK"
     gene            3026..>3481
                     /locus_tag="PKV23_04045"
     CDS             3026..>3481
                     /locus_tag="PKV23_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HQX84641.1"
                     /translation="MKESEARELIVEMARSMFERRLTFGSSGNISVKIDGGWLMTPTG
                     SSLGRLDPARLSKLDKAGALISGDAPTKESFLHLAMYRERAGTGAVVHLHSTHSVAVS
                     CLDGINHDDVLPPITAYYVMRVGRLPLIPYYAPGDKTLGEAVGRYAAKHA"
BASE COUNT          692 a          988 c         1144 g          657 t
ORIGIN      
        1 cggctatagc aaacctgaag atcaaacagg gaaagaccgg cttctcaatc agctatgacg
       61 caacctgcag cgacgcggaa cagtccctca agtaccaggc aaagatcgaa gcaagcgctg
      121 atggcaggct ggcattttct gccgtgggga cgccgctcag cgattttctc accaatcgct
      181 cggggtttgt catcctgcat ccgctggagg gggtggtggg caaacccgtc gaggtggtgc
      241 atgtggatgg cagccgcgaa aagcggaact ttccgaaaat catcagcccg ggccagccca
      301 ttttcgagat ccgttcgctg aagcatacgg cgatgcccgg ggtcaccgcc acggtgctga
      361 tggagggcaa caagttcgag atggaggacc accggaactg gatggatgcc tcctacaaaa
      421 cctatgtctg ctccctgctt gatccgtggc cctatacgct gaagaagggc gaggccttca
      481 cccagtcggt gacgcttacg atttccggga agcccacagc aaaggcatca tcaaaatctg
      541 gcggggcaat atccgtggcg atgggcaagg ccaaggggcg catgccttcg attggcgtgg
      601 gcgtaccgat ggcggaagcc cagcacgcgg ttgccaaggc cgatctcatt gctgctgcga
      661 agccggggca gttggttttc cagattgacg gacgcaagga aaaccagacg gaagcggcag
      721 cggcgtttcg cgccctgaag gaacgcacgg gcattccggc gaccttggaa attgtcctgc
      781 cggcaaaggc gccagccatg gaagaggtcg aagccattgc aggcgcgtta cgcgccgccg
      841 gtttcaagcc ggacgccgtg gtgatcacgc agatgcatga cctcaaatcc ttccagccga
      901 atacgccacg gccctggggg ccaacctatg aagagatggc ggcagcggcg cgcaaggcgt
      961 ttcccggtgt gaccctgggg ggcggcatgc tctcctattt cacggagctg aaccggaagc
     1021 ccgcgcccaa gggcgtgttt gacttcatca cccacaccgc atgccccatc gtgcatgcgg
     1081 cggatgacct ctcggtcatg gaaacgcttg aatccatgcc gtcgatcatt gcctcgacgc
     1141 gggccatgat cggcacgtcg ccctatcaca tggggccaac cagcattccc tgccgcgata
     1201 acccctacgg ggcgcgggaa gcgtccaatc ccgataatgg ccgggtgtgc ctgtccgaca
     1261 tggatccgcg gcagcgcgga ttgttcgggg cggcgtggaa tctggggctg gtagccgcgg
     1321 cggcgaaggg caagctggat gcggtttcgc tgggagcggt aacgggtcca cagggcctta
     1381 tctaccgcat ggccaattac atgcagccgg gtttggatgg cgggaacgct gcggtctatc
     1441 ccgtttatca cattcttgcg gggctggggc gcgccagcgg cgccaggcat attgaaacgg
     1501 aaagcggcgc tccgggaaaa atcgcagcgc tcgcccatga aagcaaggcg gggccggtgc
     1561 tgtggctggc aaatctcacg ggcgagaagc aagcggtgaa ggtcggcggc ttcaagggag
     1621 cggcacaggt tcatgttctc gacgaaaaga ctttcgatgg gctgatcaaa cgcccggact
     1681 acctggccaa gcccggaaca aagatgaata aggtttccag ccttgagctt gggccctatg
     1741 cggtgatccg cgtcgcggcg acctgatcaa tgctgctggg gtgcattgcc gatgacttca
     1801 cgggggccac ggacctggcg aatacgctga gccgccatgg catgcgcacg gtgcagatca
     1861 tcggcgaacc cacgggactg ccgcctgaca cggacgccat tgtcgttgct ttgaaatcca
     1921 ggactatccc tgcgaaggat gcggttgccc aatcgcttgc cgcctgccgc tggctgaagc
     1981 agcatggggc gcggcagatc tatttcaaat actgctcgac cttcgactcc accgatgcgg
     2041 gaaatatcgg cccggtagcg gatgcactgc tcgatgagct tgggtccagt ttcaccattg
     2101 cctgcccggc attcccggaa aacggccgga cgatctacat ggggcatctc ttcgtggggc
     2161 aggtgctgct ttcggactcg agcatgcggc accatccgct gacgccgatg catgattcaa
     2221 atctggtaaa tgttctggcg cggcagacgc cgcacaggac agggcttgtg gcccatgcga
     2281 cggtgaagca gggggcgggc gccattgccg ccgcctttgc agagttgcaa tcacgcggta
     2341 tccgtcacgc cattatcgat gcgatcgaag atatcgacct gcattatctc ggtgaggcgg
     2401 tagcaaatct tgatgttgtc acgggcggat cgggcgctgc cattggcctg ccggaaaatt
     2461 tccggaaggc ggggcttttg tccaagcggg agaatgtcaa gccattggct ccggttggcg
     2521 gcgggggcgt tgtgctttcg ggaagcagtt cggcggcaac gcttgcccaa gtggctgcca
     2581 tgagtgcaaa gcatcccgct tttgccatcg atgtggccgc gttattctcc aacaaggaca
     2641 aagcgatata tgaggttctt ctctgggcgg ttgagaagct gaaagcatcg ccggcgctga
     2701 tctatgccag cgcgccgcct gagaaggttt cagaaatcca atcccgattt ggccgggaga
     2761 aggcaggcgc agtgatcgag gaagccattg cggccatcgc cacgggcctg gccaagtcgg
     2821 gggtgcggcg tttcgtggtg gcgggcggcg agacttccgg tgcggtggtt tcggcgctgg
     2881 gcgtcaaggt actggaaata ggcccgcaga tttcgcccgg cgttccggcc acattatctt
     2941 atggcgagcc gcgctatgcg ctggcgctga agtcgggaaa cttcggcggg ccggagtttt
     3001 tcagcgaagc cctggaggcg ttgaaatgaa agagtccgag gcgcgcgaac ttatcgtcga
     3061 gatggcgcgc tccatgttcg agcggcggct gaccttcggc agttccggaa acatcagcgt
     3121 gaaaatcgac ggcggctggc tgatgacgcc gacgggatca tcactggggc ggctggatcc
     3181 ggcgcgcctg tcgaagcttg acaaagcggg cgcgctgatt tccggagatg cgcccaccaa
     3241 ggaaagtttt ctgcatctgg ccatgtaccg ggagcgggcc ggcaccgggg cggtggtgca
     3301 tctgcattcc acccattccg ttgccgtatc ctgcctcgac ggcatcaacc atgatgatgt
     3361 gctgccgccg atcaccgcct attacgtgat gcgggtgggc aggctgccgc tcattcccta
     3421 ttacgcgccg ggagacaaga ccctgggaga agcggtgggg cgatatgcgg cgaagcatgc
     3481 c
//
LOCUS       DAORVN010000150         3447 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_22960_length_3447_cov_3.577241, whole genome shotgun sequence.
ACCESSION   DAORVN010000150 DAORVN010000000
VERSION     DAORVN010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3447
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22960_length_3447_cov_3.577241"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..446
                     /locus_tag="PKV23_04050"
     CDS             <1..446
                     /locus_tag="PKV23_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013419281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HQX84642.1"
                     /translation="GVFRIRGTDQNFSSLLEMFGLTYPHQPYIAQKYLPDVRKGDKRI
                     ILVDGKFAGAINRVPAAHESRSNMHVGGKPEPATLTKRERDICEAMGPELKKRGLIFV
                     GIDVIGDYLTEINVTSPTGIREVKRFGGADIAVLIWDAIEERMLG"
     gene            complement(438..1259)
                     /locus_tag="PKV23_04055"
     CDS             complement(438..1259)
                     /locus_tag="PKV23_04055"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HQX84643.1"
                     /translation="MDNSRKAFIFYGITLALALAVTFTVPRIGEASLPLTMMTPTVAT
                     LIMLTVIAPEGGPRKALSLLGLDRAGLKAWPLAIAGPAAIHLAGLVILSLAGLAVFTA
                     PQMTGSVGFAIFKILSGLLIGTLFALGEEIGWRGYLLPRLLGRGVVPAMLLVGFLHGV
                     WHLPLMLTTDLYHNSGNPLLVVPLFLVTLTLAGVFFGFLRLWTGSVWAVAIAHAAANM
                     AWEIMTEMTQTKSAVVLEYVGGESGVIMIGGLLVFCFFIVRHMNSGKFKAVVESP"
     gene            complement(1252..2082)
                     /locus_tag="PKV23_04060"
     CDS             complement(1252..2082)
                     /locus_tag="PKV23_04060"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HQX84644.1"
                     /translation="MPPKETESANGLIRFVRRNQLAVFVGLAYALSWWAWIWYRLDPG
                     NVDAPILPIGPLLAALITLAVIGGWPAIRDMLRKLVHWRVGWKWYALVLLLPAALTLT
                     AFAINLLLGAQRVAGIEVPDAGQMAVRFAFIFLWIGLGEEPGWRGFALPRLLVGRSAL
                     AAAVIIGLIHMVWHAPLYGVEYDSANVWPWGISVICYSIVVCWIYLHTGGSLLMPMLM
                     HASNNTIAFVWRMFEGGDQLRLWWIWCALWVIAAIAVVLAAGPNLARDRQETQDAGLG
                     "
     gene            complement(2141..2560)
                     /locus_tag="PKV23_04065"
     CDS             complement(2141..2560)
                     /locus_tag="PKV23_04065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX84645.1"
                     /translation="MNAALLFLHLVGLMLGAAGGFASAVIMRRALVLPADEAKVVRGL
                     GPILANVSLTGLVLMWATGLILVWSKWGGIAALPDMFWLKAIFIASLTIVTGLIHMTY
                     AEIRKGNVALAARLPRLGPMAGVSALLAVLFAVLAFN"
     gene            complement(2648..2911)
                     /locus_tag="PKV23_04070"
     CDS             complement(2648..2911)
                     /locus_tag="PKV23_04070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX84646.1"
                     /translation="MMTFKEFYRREKCIALHAQSRRFRIVKYCVLLAVAVGVYVWKGL
                     AAAGLLFLVLFLAGICLHFFFRWKTEAWTKSWGLYKRIPFEGE"
     gene            complement(2950..3276)
                     /locus_tag="PKV23_04075"
     CDS             complement(2950..3276)
                     /locus_tag="PKV23_04075"
                     /inference="COORDINATES: protein motif:HMM:NF037832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HQX84647.1"
                     /translation="MRFLVDAQLPPALARRLTELGHMADHVYDIGMQAASDHSIWNRA
                     EETGAVIITKDEDFTQIGRGASGPQVVWLRLGNVNRNALLDRISKALPQIIEAIEDGE
                     RVIEIR"
     gene            complement(3279..3446)
                     /locus_tag="PKV23_04080"
     CDS             complement(3279..3446)
                     /locus_tag="PKV23_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013502391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HQX84648.1"
                     /translation="MRNMRIRVSDILDLLAAGETRADILADYPYLEDEDITAALEYAS
                     AASSHRVIAAE"
BASE COUNT          795 a         1033 c         1023 g          596 t
ORIGIN      
        1 tgggcgtctt ccgcatcagg ggaaccgacc agaatttctc ctcgctgctc gagatgttcg
       61 gcctcaccta tccgcaccag ccctacatcg cgcagaaata cctgcccgac gtgaggaagg
      121 gcgacaagcg catcatcctg gtggacggaa aatttgccgg cgccatcaac cgggttcccg
      181 ccgcccacga aagccgctcc aacatgcatg tcgggggaaa gccggagccc gccacactca
      241 ccaagcgcga gcgcgacatc tgcgaggcga tggggccgga gctgaaaaag cgcgggctga
      301 tcttcgtcgg catcgacgtg atcggcgact atctcaccga gatcaacgtc acctcgccca
      361 ccggcatccg cgaggtgaag cgcttcggcg gggcggacat cgcggtgctg atctgggatg
      421 cgatcgagga gcggatgcta gggtgattcc acaaccgcct tgaactttcc gctgttcatg
      481 tggcgaacga taaagaagca gaagaccaac agcccgccga tcatgatcac gccgctttca
      541 ccgcccacat attcgagcac gactgcggat ttggtctggg tcatttcggt catgatttcc
      601 caggccatgt tggcggccgc gtgggcgatt gccacggccc agacgcttcc ggtccacagc
      661 ctgaggaaac caaagaacac gccggcaaga gttagcgtca ccaggaacag cggcaccaca
      721 agcagcggat ttccgctgtt gtgatacaag tcggtggtga gcatgagcgg caggtgccag
      781 acgccgtgca ggaagccaac cagcagcatg gcgggcacaa cgccgcgccc cagcaggcgc
      841 ggcagcaggt agccccgcca gccgatttcc tcgcccagcg cgaagagcgt gccgatgaga
      901 agtccagaca gtattttgaa aattgcaaaa ccgaccgagc cggtcatctg cggcgcggta
      961 aatacagcaa ggccagccaa ggacaggatg acaaggcctg cgagatggat ggcggcgggc
     1021 ccggcaatcg ccagcggcca ggccttgagc ccggcacggt caagtccgag gagtgacagg
     1081 gctttgcgcg ggccgccctc gggcgcgatt actgtcagca tgatcagagt tgcgacagtc
     1141 ggcgtcatca tcgtcagtgg aagactcgcc tcaccgatcc gtggcaccgt gaaggtcact
     1201 gccaaggcaa gggccagtgt gatcccgtag aagataaagg ccttgcgtga attatccaag
     1261 gcccgcgtcc tgagtctcct gcctgtccct cgcaaggttc gggcctgcgg cgaggacgac
     1321 ggcgattgcg gcgattaccc acagggcaca ccatatccac cacaggcgga gctggtcgcc
     1381 gccttcgaac atccgccaga caaaggcaat cgtgttgttc gaggcgtgca tgagcatcgg
     1441 catcagaagg ctgcctcccg tgtgcaggta aatccagcac actacaatgg aatagcagat
     1501 gacgctgatg ccccagggcc agacattggc tgagtcatat tcaacgccgt agagcggcgc
     1561 gtgccagacc atgtgaatga ggcctatgat cacggcggcg gcaagggcgc tgcggcccac
     1621 gagaagccgc ggcagggcaa agccgcgcca gcccggctcc tcgcccaggc caatccacag
     1681 gaagatgaag gcaaagcgga ccgccatctg gcccgcatcg ggaacctcga tgcctgcaac
     1741 acgctgggct ccgaggagaa gattgatggc gaaagccgtg agggtgagcg ctgcgggcag
     1801 cagaagcacc agcgcgtacc atttccagcc gacacgccaa tgcacaagct tccgcagcat
     1861 gtcgcggatt gccggccagc caccgattac cgcaagggtg atcagtgccg caagaagcgg
     1921 cccgataggc aggatgggcg cgtcaacatt tccgggatcg aggcggtacc aaatccacgc
     1981 ccaccaggac agcgcgtaag cgaggcctac aaacacggct agctggtttc gccggacgaa
     2041 gcggatcagc ccgttcgcgc tttcggtttc tttgggcggc atggttccgg tcctcctgta
     2101 aattctatac ggacctgaaa agtggcgcgc gaaacgccag ctagttgaag gccagcacgg
     2161 cgaaaagcac ggcaagcaag gctgacacac cagccatcgg ccccagcctg ggcaggcgcg
     2221 cggccagcgc cacattgcct ttgcggattt ccgcataggt catgtggatg aggccggtca
     2281 cgatggtgag cgaggcgatg aagatggcct tcagccagaa catgtcggga agggcagcga
     2341 tgccgcccca tttgctccac accaggatca gacctgtggc ccacatcaga acaagcccgg
     2401 tgagggaaac attggccagg atcggcccga ggccgcgcac caccttggct tcatcggcgg
     2461 gcaacacaag ggcgcggcgc atgatcacgg cgctggcaaa accaccggct gcgccgagca
     2521 tcaggccgac cagatgcaga aacaggagtg ctgcgttcat cttgaattcc ccttgtcggt
     2581 gttcttcccc ggaacgttca atacatgaag tccgctgcgt tggaaaggcg gcggcagggg
     2641 aaccggatta ttccccttcg aaaggaatgc gcttgtaaag cccccaggac tttgtccagg
     2701 cttcagtttt ccagcggaaa aagaaatgca ggcaaattcc tgcgagaaaa aggacaagga
     2761 acaagagccc tgcagcggcc aaacccttcc atacataaac gccaactgcg actgcaagca
     2821 gaacgcagta tttgacaatc cgaaacctgc gcgactgcgc atggagcgcg atgcattttt
     2881 ccctgcggta gaattccttg aatgtcatca ttcgctttct tagccgataa atttccctta
     2941 tcagcgcagc tatctaatct caattacgcg ttcaccgtct tcgattgctt caatgatttg
     3001 cggtagggcc tttgaaatcc tatcgagcag agcatttcga ttcacattgc ccagccgcaa
     3061 ccaaacgact tggggaccgg aagcgccccg gccaatctgg gtgaaatctt catcctttgt
     3121 gatgataact gcgcccgtct cctcggccct attccaaatg gaatgatccg atgccgcttg
     3181 catgccgata tcgtagacat gatctgccat gtgaccgagt tctgtaagcc gtcgcgccaa
     3241 tgccggtggc aattgggcgt cgaccaagaa ccgcacggct attctgccgc gatcacgcga
     3301 tgggaagagg cggcagaagc atattccagc gccgcagtaa tatcttcatc ctcaagatag
     3361 ggataatccg cgaggatatc tgcccgggtc tctcctgcgg ccagcaaatc gaggatatca
     3421 gagacacgga tgcgcatgtt gcggatg
//
LOCUS       DAORVN010000151         3442 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_23018_length_3442_cov_2.655152, whole genome shotgun sequence.
ACCESSION   DAORVN010000151 DAORVN010000000
VERSION     DAORVN010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3442
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23018_length_3442_cov_2.655152"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(83..1024)
                     /locus_tag="PKV23_04085"
     CDS             complement(83..1024)
                     /locus_tag="PKV23_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019752941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate resistance protein"
                     /protein_id="HQX84649.1"
                     /translation="MTQWLSLITSLPTENATARMRAWRALKASGAAVLRDGVYLMPER
                     ESCRNTLDSVATDILGAGGTVFVLRVEEPDGAAFVSLFDRREDYAVLLADISKAHAGL
                     AAETAADTLKQARKLRKTFAGLAEIDFFPGEARRQAEAALQDLEQQAGWALSPDEPHA
                     VASTIPQLPLGLYLGRTWATRCRPWVDRLASAWLIRRFIDPDAHLLWLASPADCPPDA
                     LGFDFDGATFSHVGVRVTFEVLLASFGLETPALQRLGALVHFLDVGGVQPPESVGIES
                     ALAGLRDTIGDDDQLLNFASTVFDGLLASFEKATPAK"
     gene            1208..2389
                     /locus_tag="PKV23_04090"
     CDS             1208..2389
                     /locus_tag="PKV23_04090"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQX84650.1"
                     /translation="MILVNKSEGVGECKDLEDDLANLARLAAADSCEDTRLLLAKLVR
                     KYRQGRPELASRLDQSLKTTQTRSAGSSVLRRGVSVSDDREILLPVDGDSRLPLIRVF
                     DDREGLAAPILPVGVGDAIRAIIHERQESERLAARGISPTRSAILVGPPGVGKTLSAR
                     WIAQSLGKPLWVLDLTAVMSSLLGKTGNNLRMALDHAKKHAAVLLLDEIDAIAKRRSD
                     ESDIGELKRLVTVMLQEVDQWPDSGLLLAATNHPELVDPALWRRFDLVVKFDAPDAKS
                     IAVAIERFLGEDRGLFQPWIDVLAAGFQGQSLSDVERSINALRRGYTLEVAPVERLIS
                     SVLGQNQEMLSKAERLHLAIELAKAGQHTHVQISQMTGVARDTIRKHAGPSPRKGRGL
                     K"
     gene            2390..>3442
                     /locus_tag="PKV23_04095"
     CDS             2390..>3442
                     /locus_tag="PKV23_04095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S8 and S53, subtilisin, kexin,
                     sedolisin"
                     /protein_id="HQX84651.1"
                     /translation="MTTRYLIGRAELLTYPIEAPKKAPSNKAHPYTLGEAKQVIVPQI
                     EAANVLFQELPGKACVDDLAVARLVLHPAYLAKSFFPKQLFDQVGLTSVGSRTRRIKP
                     RRQVLKKAPAEADTTEIFVAGTRSALKKFPAFARSLNEQMPVGEQFAQLESFAAMTAA
                     DRLQLEGTELGHVFEVGLHLPPDGDAGYVRSLFADYAKDCGFLVNGEFGFEAGRLLFI
                     AVEGDATALERLAQFTLARVIRPMPHLRAARPVTRSQPLSVPFSLPSAEPLSREPKVA
                     VLDGGLPVEHVLGRYVRRYFLADEHALDVPSYLEHGLGGRPTLLFGTIEPEEVAARPY
                     APVDHHRVLDAKVEGED"
BASE COUNT          694 a          969 c         1074 g          705 t
ORIGIN      
        1 agccagaaaa cgaaggcttc ccagaagctg accggcgcgg gtggcgcgtc aggactcact
       61 tcaggaatgg tggtggcgtg gttcattttg cgggagtcgc tttctcgaag gaggccagca
      121 ggccatcaaa gaccgtgctg gcgaagttca ggagttgatc gtcatcgcca atggtgtccc
      181 gcaggcctgc cagggcgctc tcgataccca ccgactcggg gggctgcacg cctcccacat
      241 ccaggaaatg caccagcgcc ccaagccttt gcagcgcggg ggtttcaagg ccgaagctcg
      301 ccagcaggac ttcaaaggtg acccgaacgc cgacgtggct gaaagtcgca ccgtcgaagt
      361 caaaacccag ggcatcgggc gggcaatcgg caggcgacgc cagccacagc aggtgcgcat
      421 ccggatcgat gaagcggcgg atcagccatg cgctggcgag gcggtccacc catggacgac
      481 aacgggtagc ccacgtccgg ccgaggtaca ggccaagcgg cagttgggga atcgtgctgg
      541 cgacggcatg aggttcatct ggagacaagg cccagcctgc ctgctgctcc agatcctgca
      601 aagccgcctc ggcctggcgc cgggcctcgc cggggaagaa atcaatttca gcgaggcccg
      661 cgaaggtctt gcgtagcttg cgtgcctgct tgagggtgtc ggccgcggtc tcggcagcaa
      721 gtcctgcatg cgccttgctg atgtcggcga gcaacaccgc atagtcctca cggcggtcaa
      781 acaggctgac gaaggcggcg ccatccggct cttcgacacg caggacaaac accgtgccgc
      841 cggcacccag gatgtcggtg gccacggaat cgagcgtatt gcggcaggat tcccgttcag
      901 gcatcagata cacgccgtca cgcaggacgg cggcccctga tgccttgagc gcccgccagg
      961 cccgcatccg ggccgtggcg ttctcggtgg gaagactggt gataagcgag agccattgag
     1021 tcatgtcgtc tacgctacat cttcgttgat gtggttacaa gtaaattttg atgggcaatg
     1081 tcgcggcaca ggaaggaagg atagggatgg ctacactgca cttttcgctg catgtatccc
     1141 tgcatggctt attgacgatt tttagttttg gtcataaaat tcgccataat aatttttcat
     1201 aattctggtg attcttgtga ataaatctga aggggtgggc gaatgcaagg atctggaaga
     1261 tgatctcgcc aatttggcaa gactcgctgc cgcggactct tgtgaagaca cacgactgct
     1321 gttggcgaaa ttggttcgta agtaccggca aggtcgccct gagttggctt cacgcctgga
     1381 tcagtcgcta aagacaacac aaacccgatc ggctgggagt tctgtgcttc gacgcggagt
     1441 gtcggtgagc gatgaccggg agatcctgct gcctgttgac ggagattccc gtctgccgct
     1501 gattcgtgta ttcgatgacc gagagggatt ggcagcaccc attcttcccg taggggtagg
     1561 cgatgcgatc agagcgatca tccatgaacg tcaggagtca gagcgcttgg ctgcacgggg
     1621 tattagcccc actcgatcgg ccatcctggt cggtccgcct ggcgtgggga aaaccctgtc
     1681 tgcccgctgg attgcgcagt cgctcggaaa gccgctgtgg gtccttgatc tgacggcggt
     1741 gatgagcagc cttctgggaa agaccggaaa caatctgcgc atggcgcttg atcatgccaa
     1801 gaagcatgcg gcggtccttt tgctcgacga aattgatgcc atcgcgaagc gtcggagtga
     1861 cgaatcggat atcggtgagc tcaagcgttt ggttaccgtc atgcttcagg aggttgatca
     1921 gtggccggac agcggcctgc tgcttgctgc caccaaccac ccggaattgg tggatcccgc
     1981 gctgtggcgt cgttttgact tggtcgtgaa attcgatgca ccagatgcga aatcaatcgc
     2041 cgtcgcgatc gagcgttttc tgggcgaaga ccgggggctc ttccagccct ggatcgatgt
     2101 gctggctgca ggctttcagg ggcaatcgct ctccgatgtg gagcgaagca tcaatgcgct
     2161 tcggcgtggc tatacgcttg aagtcgcccc cgtcgagagg ttgatctcgt cagtgctggg
     2221 gcaaaaccag gaaatgctca gtaaggctga acggttgcat ttggccattg aactcgccaa
     2281 agctggacag catacccatg ttcagataag ccagatgacg ggtgttgccc gcgatacgat
     2341 tcgcaagcat gcggggccgt cgcctcgtaa aggaaggggg ctcaagtaaa tgaccacgcg
     2401 atacctgatt ggccgggcag agttgctgac gtaccccatt gaggcaccca agaaagcccc
     2461 aagcaacaag gcccaccctt acacgcttgg tgaggcgaag caggtcatcg tccctcagat
     2521 cgaagctgcc aatgtgctgt ttcaagagct tccgggcaag gcgtgcgttg atgatctagc
     2581 cgtcgcgcgg ctggtcttgc atcccgccta tttagcgaag tcgtttttcc ccaagcagct
     2641 cttcgatcag gtggggctca cgtcagtggg gtcgcggact cgccgcatca aaccacgacg
     2701 gcaggtgcta aagaaggcac cagccgaggc cgacaccaca gagatattcg ttgcaggaac
     2761 gcgctctgcc ctcaagaagt tcccggcctt cgcccgtagc ctgaatgagc agatgccggt
     2821 tggggagcag ttcgcccagc tcgaaagctt tgccgcaatg actgccgccg accggcttca
     2881 gcttgagggc acagaacttg ggcacgtctt cgaggtcggc cttcacctgc cgccggacgg
     2941 tgacgcaggc tacgtccggt cactctttgc tgactacgcc aaggactgtg gatttctcgt
     3001 caacggggag tttgggttcg aggctggtag gctgctgttt attgccgtgg agggcgacgc
     3061 gacagcgctt gaacgcttgg ctcaatttac actggctcgg gtgattcgac cgatgccgca
     3121 cctgcgagcc gcgcgccccg tcacaaggag ccagccgctc tcagtgccgt tttctctgcc
     3181 aagcgccgag ccgctttcac gggaacccaa ggttgccgtg ttggacggag gattgcccgt
     3241 tgagcacgtt ctcggtcgat atgttcgtcg ctattttctt gctgacgagc atgcactgga
     3301 tgtgccctca tacttggagc acggtctcgg tggccgccca acacttcttt tcggaacaat
     3361 tgaacccgaa gaggttgcgg cgcgtccata tgcccccgtt gaccaccacc gagtgctcga
     3421 tgccaaagtg gagggcgagg at
//
LOCUS       DAORVN010000152         3432 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_23128_length_3432_cov_3.604975, whole genome shotgun sequence.
ACCESSION   DAORVN010000152 DAORVN010000000
VERSION     DAORVN010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3432
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23128_length_3432_cov_3.604975"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            112..2544
                     /locus_tag="PKV23_04100"
     CDS             112..2544
                     /locus_tag="PKV23_04100"
                     /inference="COORDINATES: protein motif:HMM:NF024267.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQX84652.1"
                     /translation="MAVACFVFLQCRVAHAAPFQIPDIDPLMSFLTLAAVAVFFWAFR
                     VVRLLSQSENKARQRISELEIRLNEAETAFAAEAHVMIVWHGKEEFPSRVIGSMYGSA
                     KVPTVAADMLNFPAWLEADSSNVIEDCIRTLREVGKPFNIGVKTLNNELLEADGRVAG
                     GMATLRFRPLSGERRQITELNYDVRKLGMQVERLSAVLDSAPLPIWLRDGEGRLSWVN
                     RAYVKAVELPDSAAVLRSLIEVAAPDRIDTTKADPKVGLLGRAHAVFAGAMRALNIHE
                     VALPEGRAGFAVDVSELEDIEKELDRHIKAHASTLNKLDTAIAIFGPDQRLRFHNMAF
                     AQLWPLDGQWLDMHPTDGEILDRLRTQRCIPEQANYREWRAKQLSSYTTLEMRESWWY
                     LPDGRSLHVVCEQHPFGGVTYLYENVTKEIQLESRYNELIGVQRETLDNLEEAIALFG
                     SDGRLKLFNPAFSKFWNFDGAFLQKEPHVDQLVSDAAVIREAGAAWQEIKFGITGMES
                     ERRALRGRLTASDRILQYSSVPLPDGNTLLTFSDISDPAKMERALRDRTEALEAADRL
                     KNAFLSNVSYEVRTPLTSILGFAETLDLGLVGELSPKQREYVLDIKRSSEELKAIIDA
                     IIDLSAIDAGAMELKLARLDVAEVLQAVAEKLSPTMTRRDQTLSIELAEDSLAFVGDA
                     MRVEQIVSNLLSNAIGFSARGSQVRMGARRSGTNIQIWIADSGRGIDPEFQKKAFERF
                     QSKPLPGSHRGPGLGLSIVKSFTELHGGNVSLISKLDRGTTVVCSFPVDGPAKQNPRN
                     DQAERISPQRVA"
     gene            2556..>3432
                     /gene="tsaE"
                     /locus_tag="PKV23_04105"
     CDS             2556..>3432
                     /gene="tsaE"
                     /locus_tag="PKV23_04105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HQX84653.1"
                     /translation="MVIERPGLSLPAMEALAREISLWARPGLALHLSGDLGAGKSAFA
                     RAFIRGLAPDDRDFDIPSPSFSLVQSYDALRVPVVHVDLYRIGSPTEVAELGLDEFLA
                     KSLMIIEWPEKLSENLSPDCLKISISGSGAARDLKLEAAGKWIEILKRDELIKGFVKQ
                     SQVAGGTRYYFEGDASARRYERVAAKTETLLLMDMPRRPDGPVVKNGKPYSAIAHLAE
                     GIDAVVGINDYLFKLDYSVPRIYTADVNLGMALIEPLGDRVFSRMMARGEDMEEPMKA
                     AVEVLADMAKRDWPNK"
BASE COUNT          706 a         1005 c         1068 g          653 t
ORIGIN      
        1 gagtctatat ctcgggatgg gacagatgcg gcctgattcg ggcccatctc gcaggcggct
       61 ggagacgaaa tgcggcaggg caacagcaca atgaacgcaa tcatgcgtca tatggcggtt
      121 gcttgtttcg tattcctgca gtgccgcgtg gcgcatgccg cgccgttcca gattcctgac
      181 attgacccgc tgatgagctt tctcaccctg gcggcagtgg cggttttctt ctgggcgttc
      241 cgcgtcgtca gactattgag ccagagcgaa aacaaggcgc gccagcgcat cagtgaactg
      301 gagatcaggc tgaacgaggc ggagacggcc tttgccgccg aggcccatgt gatgattgtc
      361 tggcacggca aggaagagtt ccccagccgg gtcatcggca gcatgtatgg cagcgccaaa
      421 gtccccacgg tggcggctga catgctcaat tttcccgcct ggctcgaagc tgattccagc
      481 aatgtcatcg aggattgcat cagaaccctg cgggaggtcg gcaaaccctt caacattggg
      541 gtcaagacgc tcaacaatga actgctggag gccgatggac gggttgccgg cggaatggcc
      601 acactgcgtt ttcgcccgct gtccggcgaa cggcggcaga tcaccgagtt gaattacgac
      661 gtgcggaaac tgggcatgca ggtcgaacgc ctcagcgcgg tgcttgactc ggcccccctg
      721 cccatctggc tccgcgatgg ggaaggcagg ctgagctggg tcaaccgggc ctatgtcaag
      781 gcggtggaac tgcccgacag tgccgcggtg ctgcggtcgc tcatcgaagt tgctgccccg
      841 gaccgcatcg acacgacaaa ggcagatccc aaggtcggcc tcttgggccg ggcgcacgcg
      901 gtttttgccg gagccatgcg ggccctcaac atccatgaag tggccctgcc ggaggggcgg
      961 gccgggtttg cagtggatgt gtcggagctg gaggacatcg aaaaggaact cgaccggcat
     1021 atcaaggcgc atgcctcgac gctcaacaag cttgacacgg ccatcgccat cttcgggcca
     1081 gaccagcgcc tgcgctttca caacatggcc tttgcccagc tctggccgct tgacgggcaa
     1141 tggctcgaca tgcatcccac cgacggggaa atccttgacc gcctgcggac ccagcgctgc
     1201 attccggaac aggccaatta ccgggaatgg cgggccaagc agctttcctc ctatacgacg
     1261 ctcgagatgc gcgaaagctg gtggtacctg ccggacgggc gctcgctgca tgtggtgtgc
     1321 gaacagcatc catttggcgg cgttacctac ctttatgaaa acgtcaccaa ggaaatccag
     1381 ctcgagagcc gctataacga gctcatcggg gtgcagcgcg agaccctcga caatctcgaa
     1441 gaggcgatag cgcttttcgg ctccgatggc cgcctgaagc tctttaatcc ggccttctcg
     1501 aaattctgga actttgacgg cgccttcctg cagaaggagc cgcatgtgga ccagcttgtg
     1561 agcgatgcgg ccgttatccg ggaagcgggg gccgcatggc aggaaataaa attcggcatc
     1621 accggcatgg agtccgagcg ccgggcgctg cgcggccggc tgacggccag cgaccgcatt
     1681 ctccagtaca gctcggttcc cctgcccgat ggcaacacgc ttctgacgtt ttcggatatt
     1741 tcggatccgg caaaaatgga acgggcgctg cgcgaccgca ccgaagccct cgaagcggcc
     1801 gaccgcctga aaaacgcctt cctcagcaat gtctcctatg aagtccggac cccgctcacg
     1861 agcatcctgg gctttgccga aacgctggac ctcgggctgg taggcgagct ttcgccaaag
     1921 cagcgggagt atgttctcga catcaagcgc tcatcggagg aattgaaagc catcattgac
     1981 gccatcatcg acctgtcggc gattgacgcg ggcgccatgg aattgaaact ggccaggctg
     2041 gatgttgccg aggtcctgca agcggttgcc gaaaaactgt cgccgacgat gacccggcgc
     2101 gaccaaaccc tgagcataga actggcggaa gactccctgg ccttcgtggg cgatgccatg
     2161 cgggtcgagc aaatcgtttc aaacctgctg tcaaatgcca tcggattttc ggcgcgcggc
     2221 agccaggtgc gcatgggcgc caggcgctcg ggcacaaata tccagatctg gatcgccgat
     2281 tctggccgtg gcatagatcc ggaattccag aaaaaagcct tcgagagatt tcagtccaaa
     2341 cccctgccgg gcagccatcg cggccccggg ctcggcctgt caatcgtcaa gagcttcacg
     2401 gaactgcatg gcggcaatgt ttccctcatt tccaagcttg accgcggcac aactgttgtg
     2461 tgtagcttcc cggttgatgg acccgcgaaa caaaaccccc ggaacgacca agctgaacgc
     2521 atcagcccgc agcgtgttgc ctaagcccct gcatcatggt cattgaacgg cccggccttt
     2581 cccttcccgc catggaggcg cttgcacgcg agatttcgtt gtgggcaagg cccggcctgg
     2641 cactccatct ctcgggcgat ctgggggccg gaaaatccgc cttcgcccgg gcctttatca
     2701 gggggctggc gccggatgac cgggacttcg acattccgag cccgtcattt tcgctggtgc
     2761 aatcctacga tgccttaagg gtgccggtgg tgcatgtcga cctctaccgg atcggctcgc
     2821 ccacggaagt ggccgaactg gggcttgacg agttcctggc caagtcgctg atgatcatcg
     2881 aatggccgga aaagctttcc gaaaacctct ctccggattg cctgaagatc tccatctcgg
     2941 ggtcgggtgc cgcccgcgac ctgaagctgg aggcagcggg gaaatggatt gagatactca
     3001 agcgcgatga actgatcaaa ggatttgtga agcaatcgca ggtggcgggc gggacgcgtt
     3061 attatttcga gggcgatgct tcggccagac gctatgagcg ggtagcggcc aagaccgaaa
     3121 cgctgctgct gatggatatg ccgcgcaggc cggatggccc ggtggtgaaa aacggcaagc
     3181 cctacagcgc cattgcccat ctggctgaag gcattgacgc ggtggtgggc atcaatgact
     3241 atctttttaa attggactac agcgtgccgc gaatttacac ggcggatgtg aatctgggca
     3301 tggcccttat cgagccactc ggcgacaggg ttttcagcag aatgatggcg cgcggcgagg
     3361 acatggaaga gcccatgaaa gcggcagtcg aggtccttgc ggatatggcg aaaagggatt
     3421 ggccaaacaa gg
//
LOCUS       DAORVN010000153         3430 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_23158_length_3430_cov_2.848889, whole genome shotgun sequence.
ACCESSION   DAORVN010000153 DAORVN010000000
VERSION     DAORVN010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3430
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23158_length_3430_cov_2.848889"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..872
                     /locus_tag="PKV23_04110"
     CDS             <1..872
                     /locus_tag="PKV23_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002965457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HQX84654.1"
                     /translation="AGVPKGVLNLVMGRGSVVGQTMLDSRGVNAISFTGSVETGAKVA
                     MACSGRGAKYQLEMGGKNPLVVLDDANIDAAVAASIDGAFFQTGQRCTASSRLVVQSK
                     VHDAFVEKMVTAMKALKVDHALKEGTVVGPVVDENQMAQDEKYIGIARDEGGELAWGG
                     ERLNRDTKGHYLSPALFTNTKNEMRINREEVFGPVASVIKVGSYDEALAVANDTAFGL
                     SSGIFTTSLKYATDFRKKSAAGMVMVNLPTAGVDYHVPFGGRKGSSYGAREQGRFAAD
                     FYTSVKTAYTLAG"
     gene            complement(889..1497)
                     /gene="pncA"
                     /locus_tag="PKV23_04115"
     CDS             complement(889..1497)
                     /gene="pncA"
                     /locus_tag="PKV23_04115"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007610450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HQX84655.1"
                     /translation="MQTSENEVLLVVDIQHDFCSGGKLAVPDADEVVPLINDFAKNFK
                     HVVMTQDWHPAGHSSFASSHPGKDIFSEIEMAFGPQTLWPDHCIQGTHGAAFHRLLDI
                     PHTELIVRKGFRHEVDSYSAFFENDHVTPTGLGGYLKERGFTRVFCVGLAFDYCVRYS
                     AEDAKSLGFDTVVIEEACKAIDLNGTADATRSLFRDRGIELI"
     gene            complement(1505..2446)
                     /gene="glk"
                     /locus_tag="PKV23_04120"
     CDS             complement(1505..2446)
                     /gene="glk"
                     /locus_tag="PKV23_04120"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF014715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HQX84656.1"
                     /translation="MTDFSLVGDIGGTNSRFGLVKQGSMAIQHMEALRNDNFPSLEGA
                     IQQYLRNREVTSLSSAAIAVAAPVDREHISLTNYGWSFTNESLRLAAKARRLRLLNDY
                     EALALSLPHLKGDDLVQIGGQESSVPRLKIVMGPGTGLGMAALAPLPQGGWMALPGEL
                     GQVTLPTVTREEFDLRERMKKPGSIFIAEDAVSGSGLHLIYRTLSPQGKLTTPEAVIQ
                     AALKGQDAVAAKTLEHFISWLARISGDAAMALQARGGVYLAGGIAPSIIDRLKSGPFR
                     TIFEDKGKLSDIMRPIPVYVIVDKFPAFKGCAASLAQ"
     gene            2558..>3430
                     /locus_tag="PKV23_04125"
     CDS             2558..>3430
                     /locus_tag="PKV23_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012322428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HQX84657.1"
                     /translation="MSVTNQIANEVSRRRTFAIISHPDAGKTTLTEKLLLFGGAIQLA
                     GQVRAKGDRRRTRSDWMSIERARGISVVTSVMTFEYNNCVFNLLDTPGHEDFSEDTYR
                     TLTAVDAAVMVIDGAKGIEARTRKLFEICRLRDIPIITFINKMDRETREPLDLLDEIE
                     KGLALTVAPMVWPIGLGRAFVGTYDLNTPRVRRLDEDPAGQVVFGPDDPLFTTLLTPP
                     QLATWRDEMELVRDLCHSFDRDAFLEGHLSPVLFGSALRNFGVRDLLDTLIAHAPPPR
                     DQQARQRVVHASEAK"
BASE COUNT          691 a          989 c         1089 g          661 t
ORIGIN      
        1 gcgccggagt gcccaagggg gtgctcaacc tggtaatggg gcggggttcg gtggtggggc
       61 agaccatgct ggattcgcgc ggtgtgaacg ccatatcatt taccggctcg gtagagacgg
      121 gagccaaggt ggccatggcc tgttctggtc gcggcgccaa gtatcagctt gaaatgggcg
      181 gcaagaatcc gctggtggtt ctggatgacg ccaacatcga tgcggcagtt gctgcttcca
      241 tcgatggggc attcttccag accgggcagc gctgcacggc gtcttcgcgc ctcgtcgtcc
      301 aaagcaaggt ccatgacgca ttcgttgaaa agatggtcac cgccatgaag gcactcaagg
      361 ttgatcatgc cctcaaggaa ggaacagtgg tcgggcctgt ggttgacgag aaccagatgg
      421 cgcaggacga gaaatacatt ggcattgcca gggacgaagg cggcgagctg gcctggggcg
      481 gcgagcgcct gaaccgggac accaaggggc attatttgtc gccagccctg ttcaccaaca
      541 caaaaaacga gatgcggatc aaccgggaag aagtgttcgg gcccgtggct tccgtcatca
      601 aggtggggag ctatgacgag gcgcttgcgg ttgccaatga cacggcgttt ggcctgtcct
      661 ccggcatttt cacgacgtcg ctgaaatacg ccacggactt ccgcaagaaa tcggcggcgg
      721 gcatggtcat ggtcaacctg ccgacggcgg gcgtggacta tcatgtgccg ttcggcgggc
      781 gcaaaggctc aagctacggg gcgcgcgaac agggccgctt tgctgccgat ttctatacgt
      841 cggtgaagac ggcttacacg cttgcaggct gaagcgccgt aacgcggctc atatcagctc
      901 tatgccccgg tcacggaaca ggctgcgggt ggcatcggcc gtgccgttga gatcaatagc
      961 cttgcaggct tcctcgatca ccaccgtatc gaagcccagg cttttcgcgt cttcggccga
     1021 gtagcgcacg caatagtcga atgccaggcc aacgcaaaag acccgggtga atccccgttc
     1081 cttcaaatag ccgccgaggc cggtgggcgt cacatggtcg ttttcgaaga aggccgaata
     1141 ggagtcgact tcatggcgga agcctttgcg cacaatgagt tcggtgtgcg ggatatccag
     1201 cagccggtgg aaggccgcac cgtgggttcc ctgaatgcag tgatcgggcc agagggtttg
     1261 cgggccaaag gccatctcga tttccgaaaa gatgtccttt ccggggtgcg aggatgcaaa
     1321 tgaggagtgc ccggcgggat gccagtcctg ggtcatcacg acatgtttga aatttttggc
     1381 aaagtcattg atgaggggaa cgacttcatc ggcgtcgggc acagccagtt tgccgccgga
     1441 gcagaagtca tgctggatgt cgacgacaag aagaacttcg ttttcgctgg tctgcatggc
     1501 aacctcactg ggcaagggat gcggcgcatc ccttgaaagc cggaaacttg tcgacaatga
     1561 catagacggg aatgggccgc atgatatccg acaacttgcc cttgtcctcg aatatggtgc
     1621 ggaagggccc gctcttcaat cggtcaatta tggacggggc gatgccgccg gcaagataga
     1681 ctccgcccct ggcctgcagc gccatggcgg cgtcgccgga aatgcgcgcc agccaggaga
     1741 tgaaatgctc cagggttttt gccgccaccg cgtcctgccc cttcagggcg gcctgaatga
     1801 cggcttcggg cgtggttaat tttccctggg gcgaaagtgt ccggtaaatc aagtggaggc
     1861 cgctgccgct gacagcgtct tcggcgataa atatgctgcc gggctttttc atgcgctcgc
     1921 gcaagtcaaa ctcctcgcgc gtgacagtgg gcagcgtcac ctggcccaac tcaccgggca
     1981 gcgccatcca gccgccctgc ggcaggggcg ccaaggccgc catgccgagg ccagttcccg
     2041 ggcccatgac gatcttgagc ctggggacgg atgactcctg gccgccgatc tggaccaggt
     2101 catcgccctt gagatgcggc agggaaaggg cgagcgcctc atagtcattc aacagccgca
     2161 agcgccttgc cttggcggcc agacgcaggc tttcattggt aaatgaccag ccgtagttgg
     2221 tcagggaaat gtgctcacgg tcaaccgggg cggccaccgc aatggcggcg gacgacagtg
     2281 aagttacttc cctattgcgc agatattgct ggattgcccc ttcaagcgag ggaaaattgt
     2341 cgttgcgcag ggcctccata tgctggatcg ccatggagcc ctgcttaacc agaccgaagc
     2401 gggagttggt gcccccgatg tcgccgacaa gtgaaaaatc cgtcattctg tcctcagccc
     2461 tccactgcca cggccagcgg tagagggcaa gagcggcggt gatctggcta aagccttcaa
     2521 cttccgctag gaagcggctt cattcgaacc agttggaatt tccgtgacca atcaaattgc
     2581 taacgaagtt tcgcgccggc gaacctttgc catcatttcc catccggacg ccggcaagac
     2641 cacgctcacc gaaaagctcc tgctcttcgg cggcgccatt cagcttgccg gacaggtgcg
     2701 ggccaagggc gaccggcggc ggacgcgctc ggactggatg tcgatcgagc gggcccgcgg
     2761 catttcggtg gtcacctcgg tgatgacctt tgaatacaac aattgcgtgt tcaacctgct
     2821 ggacacgccg ggccacgagg acttttccga agacacttac cgcacgctga cggcagtgga
     2881 cgcggcggtc atggtcatcg acggggccaa gggcatcgag gcgcggaccc gaaagctctt
     2941 tgaaatctgc cggctgcgcg acattccaat cataaccttc atcaacaaga tggaccggga
     3001 aacccgcgag ccgcttgacc ttcttgacga gattgaaaag ggactggcgc tgacggtcgc
     3061 ccccatggtg tggcccattg gcctgggcag ggcctttgtg gggacctatg acctcaatac
     3121 gccgcgggtg cggcggctgg acgaagaccc agcaggacag gtggttttcg gtcccgatga
     3181 tcccctgttc accaccctgc tcacgccgcc gcaactggcg acctggcgtg acgaaatgga
     3241 actggtgcgc gacctctgcc acagttttga ccgagacgct tttctggagg gccatttatc
     3301 tcccgtgctt tttggctcgg ccttacggaa cttcggagtg cgcgaccttc tcgatacgct
     3361 gattgcccat gcgccgccgc cgcgcgacca gcaggcccgg cagcgggtgg tgcatgccag
     3421 cgaagcgaag
//
LOCUS       DAORVN010000154         3408 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_23405_length_3408_cov_3.253803, whole genome shotgun sequence.
ACCESSION   DAORVN010000154 DAORVN010000000
VERSION     DAORVN010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3408)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3408)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3408
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23405_length_3408_cov_3.253803"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(202..996)
                     /gene="iolB"
                     /locus_tag="PKV23_04130"
     CDS             complement(202..996)
                     /gene="iolB"
                     /locus_tag="PKV23_04130"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019862590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HQX84658.1"
                     /translation="MSTLLVKPRAGGGRVHHVTPKTARWTHVGFDLWKRKAGELATGT
                     DGDRETCLVFVSGRGSVRVGAEEYGILGGRSSPFEGRPWSVYVPPAMVWSVEATEDLV
                     LAVCSAPGKGKFPPRVIVPDVLVQETRGKGSNTRHVTNILPEWEMAENLLVVEVLTPA
                     GNTSSYPPHKHDEDNLPHESALEETYYHRLNPPQGFAFQRIYTDDRELDEAIAVEDGD
                     VTLVPRGYHPCAAIHGYELYYLNVMAGPRRTWKFHNAKEHEWLLKA"
     gene            complement(997..1881)
                     /gene="iolE"
                     /locus_tag="PKV23_04135"
     CDS             complement(997..1881)
                     /gene="iolE"
                     /locus_tag="PKV23_04135"
                     /EC_number="4.2.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011925298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050114 - myo-inosose-2 dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inosose-2 dehydratase"
                     /protein_id="HQX84659.1"
                     /translation="MTIRIGANPIGWSNDDMQEIGGWIPLEQCLSEAKAAGFQGMELG
                     NKFPRVASRLRPILEAHGLVLVGGWYSTFLLEHDAETEFAKSADHRRLLKGMGTNVFI
                     AAECTSTVHGNKQAPLSTRPVMTEAQWKTFTTRLTKLAELLKADGFQLAYHHHMGTVV
                     QTPAEIDRLMQGTGAAVNLLLDTGHAKWGGDDPVRLAKTYRTRISHVHCKDVRLAVKA
                     EADKNDWSFLDSVLAGVYTVPGDGSIDYVSVFKELKGYSGWVVVEAEQDPEKAPPAKY
                     AKMGCDNLKRFMSEAGLT"
     gene            complement(1897..>3408)
                     /gene="iolD"
                     /locus_tag="PKV23_04140"
     CDS             complement(1897..>3408)
                     /gene="iolD"
                     /locus_tag="PKV23_04140"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016823 - hydrolase activity, acting on
                     acid carbon-carbon bonds, in ketonic substances [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HQX84660.1"
                     /translation="LLPGDVFAGRRPDPVLQQVEDFGDGTASANDCFRPVSRYFDRIA
                     RPEQLLAALPRAMAVLTDPAQCGPVTLALCQDTQAEAYDYPESFFEEKIWANRRTRPD
                     EDELAEAIRVLKSAKKPVIIAGGGVLFSEASDTLAKFAEAHGIPVVETQGGKSSLPFT
                     HNLNMGAVGVTGTGASNAVCEDADVVLAVGTRLQDFTTGSWALFKNDKKTIIGLNTQV
                     FDATKHRALPLIADARVGLEELSINLGKWAAPAAWTKAAQSGFAAWMKTAGKYTDASN
                     VERPADSQVIGAVQRALGRDISVLCASGGLPGELHKLWQAGPPSSYHMEYGFSCMGYE
                     IAGGIGLKMAQPTREVVVMVGDGSYLMMNSELATSVMLGVKLIVVLLDNRGFGCINRL
                     QHASGGERFNNLFDYNTVQEVSPDIDFAAHGKSLGALSEKVSSLADLERALKKARSNT
                     RTTFIVIDTDPMKSTDAGGHWWDVAIPEVSSRSPVRAARKNYDKAKAKQRIGN"
BASE COUNT          627 a         1029 c         1055 g          697 t
ORIGIN      
        1 catcaagccg gtgaaggcag gggaatacct cacctatgac aattgcgtgc ctgacgattc
       61 actgatcgtc acgcaaatcc gcaagcgcct tgatcaagct gacgcccggt accacgaagc
      121 agccgcctga acgaaggcag cgccgcttcc gttaatccgt aagcggatta ataggaggtg
      181 attgctttcc ggcgcagcgc gctaagcctt cagcagccac tcgtgttcct tggcgttgtg
      241 gaatttccag gtccgccggg gtccagccat gacgttgagg taatacagtt catagccatg
      301 gatcgccgcg caggggtgat agcccctggg caccaacgtt acgtcgccat cctccacggc
      361 gattgcctca tcaagctcgc ggtcatcggt ataaatccgc tggaaggcaa agccctgagg
      421 cgggttgagc cggtgataat aggtttcctc cagggccgat tcatggggca ggttgtcctc
      481 gtcgtgcttg tggggcggat aggacgaggt gttcccggcc ggcgtcagga cctccaccac
      541 cagcagattt tccgccattt cccattccgg caggatgttg gtgacatggc gcgtgttgga
      601 gcccttgccc ctggtttcct gcacaaggac atcaggcaca atcacgcgcg gcggaaattt
      661 tcccttgccc ggggcggaac acacggccag caccaaatct tcggttgcct ccaccgacca
      721 gaccatggcg ggcggcacat agacggacca gggcctgccc tcaaagggtg aacttctgcc
      781 gcccagaatc ccatattcct cggcgccaac gcggacgctg ccgcggccgc tgacaaaaac
      841 cagacaggtt tcgcggtcgc catcggtgcc ggtggccagt tcgcctgctt tgcgcttcca
      901 caggtcaaag cccacatggg tccagcgcgc cgttttcggc gtcacatggt ggacacggcc
      961 gccgccggcg cggggcttta caagaagtgt tgacattcag gtcaggcccg cctcgctcat
     1021 gaaacgttta agattgtcgc atcccatctt agcatatttt gccggcggcg ccttctccgg
     1081 atcctgctcg gcctcaacca ccacccagcc gctgtacccc ttaagctcct tgaaaaccga
     1141 aacatagtca atggagccgt cgccgggcac ggtgtaaacg ccggccagca cgctgtcgag
     1201 aaatgaccag tcgtttttgt cggcttcggc cttcacggca aggcgcacat ccttgcagtg
     1261 cacgtggctg atacgagtcc tgtaggtttt cgccaggcgg acgggatcat cgccgcccca
     1321 tttggcgtgg cctgtatcga gcaggagatt caccgccgca ccagttccct gcatcagccg
     1381 gtcaatttcg gccggagtct gcaccacggt tcccatgtgg tggtggtatg caagctggaa
     1441 gccgtcagcc ttcagcagtt cggcaagctt ggtgaggcgc gtcgtgaagg tcttccactg
     1501 ggcttcggtc atcacaggac gggtggagag cggggcctgt ttgttgccat gaaccgtgct
     1561 ggtgcattcg gcggcgatga acacattggt gcccatgccc ttcaagagcc tgcggtgatc
     1621 ggcggacttt gcgaattcgg tttccgcatc atgctcgagc aggaaggttg aataccagcc
     1681 gcccaccagc acaaggccat gagcttcaag aatggggcgc agcctgctgg ccacgcgggg
     1741 aaacttgttg cccaattcca tgccttgaaa tcctgcggct ttcgcctcgc tcaggcattg
     1801 ctccagcgga atccagccgc cgatttcctg catgtcgtca ttggaccatc cgatggggtt
     1861 ggcgccgatc cgtatcgtca taggtgctgc tttctctcaa ttgccgatgc gttgtttggc
     1921 tttcgccttg tcgtaatttt tgcgcgcggc gcgtaccggg ctccgcgatg agacctcagg
     1981 gatcgccaca tcccaccagt ggccgcccgc gtcggtggat ttcatcgggt ccgtgtcaat
     2041 cacgatgaag gtggtgcggg tgttgctccg ggccttcttc agggcgcgtt cgagatcggc
     2101 gagagaggat actttttccg agagcgcccc gaggctcttg ccatgggcgg cgaaatcaat
     2161 gtcgggggaa acctcctgca ccgtgttgta atcaaacagg ttgttgaaac gctcgccgcc
     2221 tgaggcatgc tgcaggcggt tgatgcagcc gaagccgcgg ttgtcgagca gcaccacaat
     2281 gagtttcacc ccgagcatca ccgaggttgc aagttcggag ttcatcataa gataggaacc
     2341 gtcgcccacc atcacaacaa cttcgcgggt tggctgggcc atcttgaggc caatgccgcc
     2401 ggcgatttca tagcccatgc aggaaaagcc atattccatg tggtaggacg aaggcgggcc
     2461 cgcctgccag agcttgtgca gttcgccggg caggccgccc gaggcgcaga gcacggatat
     2521 gtcacggcca agggcgcgct gcacggcgcc gatcacctgc gagtccgctg ggcgctccac
     2581 attgctggca tcggtgtatt tgcctgcggt tttcatccag gcggcaaatc cgctttgcgc
     2641 cgctttggtc caggcggcag gcgctgccca ttttccgaga ttgatggaga gttcctcaag
     2701 gcctacgcgg gcgtcggcaa tcaagggcaa agcgcggtgc ttggtagcgt caaagacctg
     2761 ggtgttgagt ccaatgatgg tctttttatc attcttgaac agagcccagg agccggtggt
     2821 gaaatcctgc agccgcgtgc cgaccgccag caccacgtcc gcatcttcgc atactgcgtt
     2881 ggaggccccg gtgccggtga cgcccaccgc ccccatgttc aaattatggg tgaagggcag
     2941 ggaggatttt ccgccctggg tttcaacgac gggaatgcca tgagcctcgg cgaatttggc
     3001 cagggtgtcg gaagcctccg aaaacagcac gccgccgccg gcgatgatca cgggtttctt
     3061 cgcgctcttc agaacgcgaa tggcttcggc cagttcgtcc tcatcggggc gggtgcggcg
     3121 gttggcccag attttttcct cgaagaagct ttcgggatag tcgtaagcct cggcctgggt
     3181 gtcctggcac agcgccagcg tgaccgggcc gcattgcgcg gggtcggtca gcaccgccat
     3241 ggcgcggggc agggcggcca gcagctgctc cgggcgggcg atgcggtcga aatagcggga
     3301 gaccgggcgg aagcagtcat tggcggacgc cgtgccgtcg ccgaaatcct cgacctgttg
     3361 cagcaccggg tccggccggc gcccggcgaa gacgtcgccg ggcagcag
//
LOCUS       DAORVN010000155         3390 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_23621_length_3390_cov_2.232684, whole genome shotgun sequence.
ACCESSION   DAORVN010000155 DAORVN010000000
VERSION     DAORVN010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3390
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23621_length_3390_cov_2.232684"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1296
                     /locus_tag="PKV23_04145"
     CDS             <1..1296
                     /locus_tag="PKV23_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HQX84661.1"
                     /translation="VVEEAPSPLLDPKTRKAMGEQAVALAKAVNYDSAGTVEFVAGQD
                     RSFFFLEMNTRLQVEHPVTELVTGVDLVEQMIRVAYGEKLALRQADVRLNGWAIESRI
                     YAEDPYRNFLPSVGRLVKYRPPAEQISDGVTLRNDTGVFEGGEISIWYDPLIAKLCTH
                     APTRAGAITAMAQGLDGFYIDGIQHNVPFVSAIMDNPRWKEGTLSTGFIAEEFPDGFK
                     GNAITPELADRLTTVACVLDHVGNRRKRKISGQMSGRAVKFSRGRKVKLGDEWYDAEV
                     VHETERLIQILLPGERGERAIVSQWTPGLPVWHGIIDGEEISVQVRRISNGYRLSHRG
                     VSIDARVYAPREAELVKLMPKKTAADTSKKLLCPMPGLVVALHVGEGAEVKAGDALAI
                     VEAMKMENILRAERDGTVKKINAKPGDSLSVDAVIMEFV"
     gene            complement(1280..1954)
                     /gene="lipB"
                     /locus_tag="PKV23_04150"
     CDS             complement(1280..1954)
                     /gene="lipB"
                     /locus_tag="PKV23_04150"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007565992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HQX84662.1"
                     /translation="MTARLAIQPKLQSAIPVRWEIAPDPVPYDVAVARMEHEVEAIAK
                     GDAAELVWLMEHPPLYTAGTSAKDSDLIMPALFPVHHTGRGGQYTYHGPGQRVAYVML
                     DLKKRGQDVRAFVAGLEEWLIATLAAFNVKGERREDRVGVWVKREGREDKIAAIGIRV
                     RRWVSYHGISLNVDPDLRHFDGIIPCGVREHGVTSFVDLGLPVTLADADVALKNCFQD
                     IFTQTP"
     gene            2052..>3390
                     /locus_tag="PKV23_04155"
     CDS             2052..>3390
                     /locus_tag="PKV23_04155"
                     /inference="COORDINATES: protein motif:HMM:NF021606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HQX84663.1"
                     /translation="MALAGFVLALIALNRISRLTTQIAQLKLQVGLLSDQGAGAAPAP
                     KESPKAEKKKSNWPTTAVAAAKEPEPVLAETPDPDLQAQAAKTLESVPEKKPTAAKQD
                     MEQQLASRWFVWIGGVAIAIGGLLFVKYAYDNGLISPPLQIVLGLLLGAALVFAGDTL
                     HRRTPAAAQPNYVPAALSAAGLSTAFGSIYAAYALYELVAPTTAFIGLALLALGALAL
                     SLRQGPFIAALGLLGSNVTPMMISSPDPSGWSFFPYLLIILGASFAVLRKRPWWWLGY
                     AGISGSALWAFLWLGGPFEVSDTVPIGLFALCIGAVSLFAISGRSILGSASGSLLNPM
                     GMTDSLKIGSIGGVAAALVLAALVVKSGHSGLALTLFFIGMAGIAALSWCKQGDTAAA
                     PAAALLSFIVLMVWEQAAFNEWAMDESGLWSSILGGDAQPYLRWMLAAGTAFTA"
BASE COUNT          627 a         1081 c          992 g          690 t
ORIGIN      
        1 gtggtcgagg aagccccttc gcctctgctt gatcccaaga cccggaaggc gatgggcgag
       61 caggccgtgg ccctggccaa agctgtcaac tacgacagcg ccggcacggt cgagttcgtc
      121 gccgggcagg accgctcctt cttttttctt gaaatgaata cgcggctgca ggttgaacat
      181 ccggtcaccg aactggtgac gggcgtcgac ctcgttgagc aaatgatccg tgtggcctac
      241 ggcgaaaaat tggctctccg ccaggctgac gtgagactga acggctgggc catcgaaagc
      301 cgcatctatg ccgaagaccc ttaccggaat ttcctgccgt cagtgggccg cctggtgaaa
      361 tatcgtccgc ctgcggagca aatcagtgat ggcgtgacac tccgcaacga cacgggcgtg
      421 ttcgaaggcg gcgagatctc catctggtac gatccgttga ttgccaagct ctgcacccac
      481 gcgcccacgc gggccggggc catcaccgcc atggcgcagg gcctcgacgg attctacatt
      541 gatggcatcc agcacaatgt accctttgtt tccgccatca tggacaatcc gcgctggaag
      601 gagggaacgc tttcaaccgg cttcatcgcc gaggaatttc ccgatggctt caagggcaat
      661 gccatcaccc ctgaactcgc cgaccggctc accaccgttg cctgcgtgct cgaccatgtt
      721 ggaaaccgcc gcaagcgcaa gatctccggc cagatgagcg gccgggctgt caaattctca
      781 cgtgggcgca aggtgaagct cggggacgag tggtatgatg ctgaggttgt tcatgaaacc
      841 gaacgtctga tccaaatcct cctgcccggc gagcgcggcg agcgcgccat cgtcagccag
      901 tggacgccgg gattgcccgt atggcacggc attatcgatg gtgaggaaat ctccgttcag
      961 gtccgccgca tttccaatgg ctaccgcctc agccatcgcg gcgtgagcat agacgcccgg
     1021 gtctatgcgc cgcgcgaagc ggaactcgtg aagctcatgc cgaagaaaac ggcggctgac
     1081 acctccaaga aacttctctg ccccatgccg ggactcgtcg tggcgttgca tgtgggcgag
     1141 ggagcggagg taaaggcggg cgatgcgctc gccatcgtcg aagccatgaa gatggaaaac
     1201 atcctgcgcg ccgagcgcga cggcacggtg aagaaaatca acgccaagcc cggcgacagc
     1261 ctcagcgtcg acgccgtgat catggagttt gtgtgaagat atcctgaaag caattcttca
     1321 gcgccacatc cgcatcggcc aaggtcacag gcaagccaag gtcaacaaag ctggtcaccc
     1381 catgctcccg caccccgcag ggaatgattc cgtcaaaatg ccggagatcg ggatctacat
     1441 tcaggctgat gccgtgatag ctcacccagc gccggacccg gattccgatg gccgcgatct
     1501 tgtcttcgcg gccctcgcgc ttcacccaga cgccgacccg gtcctcgcgt cgttcgcctt
     1561 tgacgttgaa cgcggcaagc gtcgcaatca gccattcctc cagtcccgcc acaaaggccc
     1621 gcacatcctg tccgcgtttc ttgaggtcaa gcatgacata ggctacccgc tgcccgggcc
     1681 cgtgataggt gtattgcccg ccgcgccccg tgtgatgcac cgggaatagg gccggcataa
     1741 tgaggtcgct gtccttggcc gaagtgcctg ccgtgtagag cggcggatgc tccatcagcc
     1801 acaccagttc cgcagcatcg ccttttgcaa tcgcttccac ttcatgttcc atccgtgcca
     1861 ccgccacatc atagggaacg gggtcagggg caatttccca gcgcacagga atagcacttt
     1921 gcagttttgg ctgaatggca aggcgggcag tcatttccgg tcttttccca atttcgacaa
     1981 tcaaaggcaa gcggtgtaac ttcgcccgac tcaatggctc aggggattcc atggacggct
     2041 ttggatggtt gatggcactt gccggtttcg ttctggccct catcgccctt aacaggattt
     2101 cgcgccttac cacacaaatc gcccagctga aattgcaggt ggggctgctt tccgatcagg
     2161 gggcgggcgc ggcccctgcg cccaaggaat cacccaaggc ggaaaagaag aaatcgaact
     2221 ggcccaccac ggccgttgcc gccgctaaag aacctgaacc tgtcttggct gaaacaccag
     2281 atccggacct gcaggctcaa gcggccaaaa cccttgaatc agttccggag aaaaaaccca
     2341 cggcggcgaa gcaggatatg gaacagcaat tggcctcgcg ctggttcgtc tggatcggcg
     2401 gcgtggccat tgccatcggc ggtctgctct tcgtcaaata cgcctatgac aacggcctca
     2461 tttcgccgcc cctgcagatt gtcctgggcc tgctgctggg cgctgccctg gttttcgccg
     2521 gcgacaccct gcaccgcaga acgcccgccg ctgcgcagcc caactatgtg cccgccgctt
     2581 tgtcggcagc gggcctttct actgctttcg gcagtatcta cgccgcctat gcgctctatg
     2641 aattggtggc gccgacgacc gccttcatcg gattggcgct gctggctttg ggggcgttgg
     2701 ccctctcgct tcgccaggga ccgttcattg ccgcccttgg cctcctgggt tccaacgtga
     2761 cgcccatgat gatttcctcg ccggatcctt cgggctggag cttctttccc tatctcctga
     2821 tcattctcgg cgcgagcttt gcggttctcc gcaagcggcc ctggtggtgg ctgggctatg
     2881 ccggcatcag tggctcggcc ctttgggcgt tcctctggct cggcgggccc ttcgaagttt
     2941 ctgatactgt gccaatcgga ctgttcgccc tgtgcatcgg cgccgtgtcg ctctttgcaa
     3001 tctcgggccg cagcattctg ggcagcgcct cgggctcgct tctgaacccc atgggcatga
     3061 ccgactcgtt gaaaattggc tccatcggcg gcgtcgcggc ggcgctggtt ctcgcggctc
     3121 tcgtcgtgaa aagcggccat tcaggcctgg ccctcaccct gttcttcatc ggcatggcag
     3181 gcattgccgc cctctcgtgg tgcaaacagg gcgacacggc tgcagcgccc gccgccgctc
     3241 tcctgtcatt cattgtcttg atggtctggg agcaagccgc tttcaacgaa tgggccatgg
     3301 atgaaagcgg cctttggtcc agcatattgg ggggcgatgc ccaaccctat ctccgctgga
     3361 tgctcgccgc cggcacggct ttcaccgcac
//
LOCUS       DAORVN010000156         3387 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_23659_length_3387_cov_3.072029, whole genome shotgun sequence.
ACCESSION   DAORVN010000156 DAORVN010000000
VERSION     DAORVN010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3387
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23659_length_3387_cov_3.072029"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..128
                     /locus_tag="PKV23_04160"
     CDS             <1..128
                     /locus_tag="PKV23_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007629587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX84664.1"
                     /translation="YRQARGTANLIQAQRDFFGAHGFKRLDGEGDFHGPWGSGNN"
     gene            143..772
                     /locus_tag="PKV23_04165"
     CDS             143..772
                     /locus_tag="PKV23_04165"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009451071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HQX84665.1"
                     /translation="MSLPKRLLDGYANFRKGRYASESERYRKLGEGQQKPKIMMIACC
                     DSRAAPETIFDAGPGEMFVVRNVANLVPPYTPDGGHHATSAALEFAVLSLAVEHIVVM
                     GHGRCGGIRAAVGDGSPLSHTDFIGSWMRPIKDVARAVHPGDNAALHERLIERASIEH
                     SIANLRTFPWIRMKETRKELALHGSWFDVSMGELHAYDQSEATWQLIEV"
     gene            complement(776..1051)
                     /locus_tag="PKV23_04170"
     CDS             complement(776..1051)
                     /locus_tag="PKV23_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018700377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HQX84666.1"
                     /translation="MIEGESRTSGVVLFKGPNGQSESGIWICTPGFWNCHVTSDEFCH
                     FLLGRCTYTHESGEVIEIVPDTVAFFPKDWRGTCKVHETIRKVYMIR"
     gene            complement(1137..1502)
                     /locus_tag="PKV23_04175"
     CDS             complement(1137..1502)
                     /locus_tag="PKV23_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018700378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HQX84667.1"
                     /translation="MPTKLDSSAPHLERPATFAGPLKDWGHQPNPIAGNSKNDGLLLH
                     KGPDGQPEVGLWQCTEGTWPLEIPRDELCHFVSGRATYTHESGDVIDVSAGTVVLFPA
                     GWKGTCTVHQTMRNVYMLR"
     gene            complement(1502..2857)
                     /locus_tag="PKV23_04180"
     CDS             complement(1502..2857)
                     /locus_tag="PKV23_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010912248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQX84668.1"
                     /translation="MAKTPKPKKPAKKSKAAKKAKKVIALFPEAAFGPALNSVGIGQA
                     LKKLGHKVVFLSDPGFLDVYRGYGFEAHAVNLSEPMPAEEMAKFWVDFINGHIPNFRK
                     SPYDQIDNYVKGCWEMIVETSKWAEKDLPGVLAKVKPDVVCVDNVILFPAIKRYARDN
                     RKPWVRVISCSENEIEDPDIPPHLSGCGEKDKKGFAKYRKHFNATIKPIHDAFNAFLK
                     SVGEKKYPLGQFFEASPHMNILLYPTAAKFKRRHKLSPKQFQYLEGCVRKDAPYKVPQ
                     FPANNDKPLLYVSYGSLGSGDTELLKRVIKVIGKMPVRALVNVGDYLDQYTDIPPNVL
                     IDKWYPQPSVIPKVDGVIHHGGNNSFTECLYFGKPAIIMPYVWDGHDNATRVEETGHG
                     FKVDRYTWTDDELAAKIMAMLTDKKMKAKLQKTSKAMRAEHGPTKAAKIIDGLTRRKI
                     A"
     gene            2961..>3387
                     /locus_tag="PKV23_04185"
     CDS             2961..>3387
                     /locus_tag="PKV23_04185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX84669.1"
                     /translation="MLEIFSLTDLIGTLSYALIALSYMMTRILWLRVVAVVGLLLEIA
                     YFRFSGGDLKVGIGWNSIFILINLFLLVLLVRERASLRLPEKDAPMLRQSFVGLDDAQ
                     IARLLKAADWRDGQPGEILTQQDHAVDSLFFLCSGRASVE"
BASE COUNT          610 a         1048 c          980 g          749 t
ORIGIN      
        1 gctaccgcca ggcgcgcggc accgccaatc tcatccaggc ccagcgcgat ttcttcggcg
       61 cccacggctt caagcggctt gacggggaag gcgacttcca cggcccttgg ggcagcggca
      121 acaattgatt caggaggaag caatgtccct gccaaaacgg cttctcgatg gctatgcgaa
      181 tttccgcaaa gggcgttacg cgtccgaatc ggaacgctat cgcaagctgg gcgagggcca
      241 gcagaagccc aagatcatga tgatcgcctg ctgcgattcc cgcgccgcgc cggaaaccat
      301 tttcgacgcc ggcccgggcg aaatgttcgt ggtgcgcaat gtcgccaatc ttgtgccgcc
      361 ctatacgccc gatggcggcc accacgccac cagcgccgcg ctcgaattcg cggttctctc
      421 gctcgccgtt gagcatatcg ttgtcatggg ccatggccgc tgcggcggca tccgcgccgc
      481 cgtgggcgat ggaagtcctc tcagtcacac ggacttcatc ggcagctgga tgcggccaat
      541 caaggacgta gcccgcgccg ttcaccccgg cgacaatgcc gccctgcatg aacgcttgat
      601 cgagcgcgcc tccatcgaac attccatcgc caatttgcgc accttcccgt ggatccgcat
      661 gaaggaaacc cgcaaggaac ttgccctgca cggcagctgg ttcgatgtca gcatgggcga
      721 gctccacgcc tatgaccaga gcgaggcgac ctggcagctt atcgaggtct agatcctagc
      781 ggatcatgta aaccttgcgg atggtttcat gcaccttgca ggtgccgcgc cagtccttcg
      841 ggaaaaacgc caccgtgtcc ggcacgatct cgatcacctc gccggattca tgtgtgtagg
      901 tgcagcgccc cagcaggaaa tggcagaact cgtcgcttgt cacatggcag ttccagaagc
      961 ccggcgtgca gatccagatg ccgctttcgc tctggccatt ggggcccttg aagagcacca
     1021 ccccgctggt gcgcgattcg ccctcgatca tcgtggggat cacgccccag tccttgaacg
     1081 ggccttcata ggtggcggga tttttcagct gcggggcgga agatgtcagg ttcattctat
     1141 ctcaacatgt aaacattgcg catggtctgg tgaacggtgc aggtgccttt ccagccggcg
     1201 ggaaacagca ccacggttcc ggcggacacg tcaatcacat cgccgctctc atgggtgtag
     1261 gtggcgcggc ccgatacgaa gtggcacagc tcatcgcgcg ggatttccaa aggccacgtg
     1321 ccctccgtgc attgccacag ccccacttcc ggctggccat ccggcccttt gtgcaagagc
     1381 aggccatcat tcttcgaatt cccggcaatc ggattgggct gatggcccca atccttcagc
     1441 gggcccgcga atgtcgccgg ccgttcaaga tgcggcgccg aactgtcgag ctttgttggc
     1501 atcaggcgat cttccgccgc gtcaggccat caatgatctt cgccgccttg gtcggcccat
     1561 gctcggcccg catggctttt gaggttttct gcagcttcgc cttcattttc ttgtccgtca
     1621 gcatggccat gatcttggcg gccaactcgt catcagtcca ggtgtagcgg tcaaccttga
     1681 agccgtggcc ggtttcctca acccgcgtgg cgttgtcatg gccgtcccag acatagggca
     1741 tgataatggc gggcttgccg aaatagaggc attccgtgaa ggagttgttg ccgccgtggt
     1801 ggatcacgcc gtcgaccttc gggatcaccg aaggctgcgg ataccacttg tcgatcagca
     1861 cattgggcgg aatatccgta tactgatcaa gatagtcgcc gacattgacc agggccctca
     1921 ccggcatctt cccgatgacc ttgatcaccc gcttcagcag ttccgtgtcg cccgagccca
     1981 gcgagccata gctcacatag agcaggggct tgtcgttgtt cgccgggaac tgcggcacct
     2041 tgtagggcgc gtccttccgc acgcagccct cgagatactg gaactgcttg ggcgaaagct
     2101 tgtgccgccg cttgaatttc gccgcggtgg gatagagcag gatgttcatg tgcgggctcg
     2161 cttcgaagaa ctgccccagc ggatatttct tctcgcccac gcttttcagg aaggcgttga
     2221 aggcgtcatg gattggcttg atcgtggcgt tgaaatgctt gcggtatttg gcaaagccct
     2281 ttttgtcctt ctcgccgcag cccgaaagat gcggcggaat gtccggatcc tcgatctcgt
     2341 tttccgagca ggaaatgacc cgcacccagg gcttcctgtt gtcccgggca tagcgcttga
     2401 tcgccgggaa caggatgaca ttgtcgacgc agacgacatc gggcttcacc ttggcaagca
     2461 cgccgggcag atccttctcc gcccatttcg acgtctcgac gatcatctcc cagcagccct
     2521 tcacgtaatt gtcgatctgg tcgtaggggc tcttgcggaa attggggatg tggccgttga
     2581 tgaaatccac ccagaacttg gccatttcct ccgccggcat gggctcggac agattgaccg
     2641 catgcgcctc gaagccatag ccccggtaga catcgagaaa gccggggtcc gacaggaaca
     2701 ccaccttgtg gccgagtttc ttcagggcct ggccgatgcc aaccgaattc agcgccggcc
     2761 cgaaggcggc ttcggggaaa agggcgatca ctttttttgc ttttttcgcg gctttcgatt
     2821 ttttggcggg ttttttcggt ttcggcgttt tcgccattgc actctccctt gaattgaaac
     2881 tcttctagcc tcccgtctct tggcttgaca agccacaagc taaaaagcga acttggcgcc
     2941 cgaaccggaa ttgggcgcct atgctggaaa ttttttcact gaccgatctg attggcacgc
     3001 tgtcctacgc cctcattgcg ctgtcctaca tgatgaccag gatcttgtgg ctgcgcgttg
     3061 ttgctgtcgt gggcctgttg cttgaaatcg cctatttccg tttctccggc ggtgacctga
     3121 aagtcggcat cggctggaac tccatcttca tcttgattaa cctttttctg cttgtcctcc
     3181 tggtgcgcga gcgcgccagc ctcaggctgc cggagaagga tgcgccgatg ctgcgccagt
     3241 cctttgtggg cctcgatgac gcgcagatcg ccaggctgct caaggccgcc gactggcgcg
     3301 atggccagcc cggcgaaatc ctcacccagc aggaccatgc ggtggactcg ctgttcttcc
     3361 tctgctcggg ccgcgccagc gtcgagg
//
LOCUS       DAORVN010000157         3375 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_23802_length_3375_cov_3.064157, whole genome shotgun sequence.
ACCESSION   DAORVN010000157 DAORVN010000000
VERSION     DAORVN010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3375)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3375)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3375
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23802_length_3375_cov_3.064157"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1642
                     /gene="htpG"
                     /locus_tag="PKV23_04190"
     CDS             <1..1642
                     /gene="htpG"
                     /locus_tag="PKV23_04190"
                     /inference="COORDINATES: protein motif:HMM:NF003555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HQX84670.1"
                     /translation="DKAAKTLTIADSGIGMGEDELIDNLGTIARSGTQAFVEKAGQGV
                     NLIGQFGVGFYSSFIVANRVEVTSRRAGTDTSFTWASEGLGTFTVVPAEKGPRGTSIV
                     LHLKDDALEFLEGWKIEQVIRAYSDHIGHPIMLADGSEAAKQINTANAIWTRPKAEVT
                     AEQHKEFFGHVTGNYSDPAMTIHYRAEGRHEYTVLLYVPTERPFDLYDPERRGRQKLY
                     VKRVYIADDAQLLPPYLRFVRGVIDSEDMPLNLSREMLQHNRQVAAIRKAVTNKVLGE
                     IKKFSEADPEKFLKMWEIFGPVIKEGLYEDFEHRGQLFEFARFRSTRNASVTLKDYVA
                     GLKENQTAIYYLTGEDELKAAASPQLEGYRARDVEVLLLTDPVDSFWVRTAMEFDGKP
                     FKSVTQGTADLDLIAVKDAPPKTEVKESATAKLIVAMKEALGDKVKDVRSSARLTDSA
                     VCLVADGELDRTLEKLLSRQKDSGVNVSAPVLEINAGHPLIVALAAAADAGASPPEIA
                     DAAQLLLDQAHVLEGEPVSDPAGFSRRMANVLTRAFAK"
     gene            1947..2147
                     /locus_tag="PKV23_04195"
     CDS             1947..2147
                     /locus_tag="PKV23_04195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX84671.1"
                     /translation="MFSLKSNLFVSMLAEAKDYAKKINLRRSHPPNFEADPRELNRML
                     SELRLAREHAQLMNVMHRNRIF"
     gene            complement(2144..3085)
                     /locus_tag="PKV23_04200"
     CDS             complement(2144..3085)
                     /locus_tag="PKV23_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013529407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HQX84672.1"
                     /translation="MTVTRATISEAATRIAPFIRRTPVMDITLPGIENPVCLKLELFQ
                     HTGSFKPRGAFTNLVGVKIPGAGVAAASGGNHGAAVAYAASVLGIKARIFVPVISSPA
                     KVARIAGYGATIVQEGANYQAALALCEDFVRESGAVNIHAYDMETTLAGQGTLGRELE
                     EQAPGLDRVLVAVGGGGLIGGIAAWYQAKSKIIGVEPRTCNCLHAALAAGSTVTIKPS
                     GLATDSLGASAAGSLMFPIAQKFVDHIALVTDEDIRNAQRWLWSHAQIVAEPGGATAL
                     AALLSGSYRPAKGARVGVIVCGANTPLATFNELFTTA"
     gene            complement(3082..>3375)
                     /locus_tag="PKV23_04205"
     CDS             complement(3082..>3375)
                     /locus_tag="PKV23_04205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX84673.1"
                     /translation="ATLPHKEILATSIPVGHGNLATLAITGSDYAETISTVKVEVPHG
                     TGDFLAGLYFASRLNGFAPPEALKLSSGILESAISRSLGQPCLDIIGALHSPS"
BASE COUNT          709 a          941 c         1049 g          676 t
ORIGIN      
        1 cgacaaggcg gccaaaacgc tcacaatcgc cgacagcggc atcggcatgg gcgaggacga
       61 gctcatcgac aatctgggca ccatcgccag gtccggcacc caggcatttg tggaaaaggc
      121 gggccagggg gtcaatctca tcggccagtt cggggttggt ttctactcgt ccttcatcgt
      181 ggcaaaccgc gtcgaggtca cctcgcgccg ggccggaacg gacacttcct tcacctgggc
      241 ttccgagggc ctgggtacat tcacggtcgt tccggcggaa aaggggccgc ggggaacgtc
      301 catcgttctg catctcaagg atgacgcact tgaattcctt gagggctgga aaattgaaca
      361 ggtgatacgg gcctattccg accacatcgg ccatccgatc atgctggctg atggcagcga
      421 agcggccaag caaatcaaca cggccaatgc catctggacc cggcccaagg ccgaggtcac
      481 ggcggaacag cacaaggaat tcttcggcca cgtcacggga aactattccg atccggccat
      541 gaccatccat taccgggcgg agggacggca tgagtacacg gtgctgctct atgtgccgac
      601 cgaacggccg tttgacctct atgacccgga gcgccgcggc cggcagaaac tttatgtgaa
      661 gcgggtctac attgccgatg acgcgcaact cctgcctccc tacctgcgtt tcgtgcgtgg
      721 cgtgattgat tcagaggata tgccgctcaa cctctcgcgc gagatgctgc agcacaaccg
      781 ccaagtcgcg gcgatccgca aggcggtgac caacaaggtg ctcggcgaga tcaagaagtt
      841 ctccgaagcg gatccggaaa aattcctgaa gatgtgggag atcttcggcc cggtcatcaa
      901 ggaagggctc tatgaggact ttgagcaccg cgggcagctg tttgagttcg cgcgcttcag
      961 gtcgaccagg aatgcttcgg ttaccttgaa ggactatgtg gcgggcctca aggaaaacca
     1021 gacggcgatc tactatctga caggcgagga tgagctgaag gcagcggcca gcccgcaact
     1081 tgaaggctac agggccaggg atgttgaggt cctgctcctc accgacccag tcgattcctt
     1141 ctgggtgcgc acagctatgg agtttgatgg caagcccttc aagtcggtca cgcaagggac
     1201 ggccgatctt gatctgatcg cggtgaagga tgcgcctccc aagaccgaag tgaaggaatc
     1261 ggcaactgcc aaactgatcg tggcgatgaa agaggcgctg ggcgataagg tgaaggatgt
     1321 gcgcagttcc gcacgattga ctgacagtgc ggtttgcctg gtggccgacg gcgaattgga
     1381 ccgcaccctg gaaaagcttc tgtcgcgcca aaaggattca ggcgtgaatg tgagcgcccc
     1441 ggtgctggaa ataaacgccg gccatccgct tatcgtggcc cttgcggcgg cggcggatgc
     1501 aggcgcttcg ccgcctgaaa tagctgatgc ggcgcagctc ttattggatc aggctcatgt
     1561 gcttgagggc gaaccggtga gcgatccggc gggcttttca cggcgcatgg cgaatgtgct
     1621 gacgcgggct tttgccaagt gatgtgtctg ccctgcaaca gtgtttggca ttttttcaga
     1681 aaactctttt cagatatgat attttcgcgt acagcgacgg cgactgtaac tgaaagggag
     1741 atcatcatga caaggacatt ttcgcgctgc tgatccccag gctgtcgagg cttgggcccc
     1801 ggcagatagc gttacccgcg cccgaggcgc aacttaatca aatcataact taactgtcag
     1861 gttttctgaa acgtaagtgt ttacgtcttt gaggccgtga ctcattttcg cccgcgattc
     1921 atgcgcgtct ggctacggag aatcttatgt tttcgcttaa atcaaacctg tttgtttcca
     1981 tgcttgctga agcaaaagac tatgccaaga agatcaatct gcggcgcagc catccgccca
     2041 attttgaggc tgacccgcgc gagttgaacc ggatgctgtc cgagctccgg ctggcgcgcg
     2101 agcacgcgca actcatgaac gtgatgcacc gcaaccggat tttttaggct gtggtgaaca
     2161 gctcattgaa ggttgcgagc ggcgtgttgg cgccacatac gatgacgccg actcgcgcgc
     2221 ccttggccgg cctgtaactt cccgacagca gcgcggcgag ggccgtggcc ccgccgggtt
     2281 cggctacgat ctgtgcatgg ctccagagcc agcgctgggc attgcggatg tcttcgtcgg
     2341 tgacaagggc aatatgatca acaaactttt gcgcgatggg gaacatgagt gagcctgccg
     2401 cacttgcgcc cagactgtcg gtggcaagcc ccgatggctt gatggtgacg gtgctgcctg
     2461 cggccaaggc cgcatgcagg caattgcagg tccgaggttc gacgccgatg atttttgatt
     2521 ttgcctgata ccaggcggcg atgccgccga tcaggccacc cccgccgacg gccacaagaa
     2581 ccctgtccag gccgggcgcc tgttcctcaa gttcgcggcc caaggttccc tggcccgcaa
     2641 gcgtcgtttc catgtcatag gcgtgaatgt tcacggcgcc gctttcacgg acaaagtcct
     2701 cacagagcgc cagggccgcc tgataattgg cgccttcctg cacgatggtg gcgccatagc
     2761 ctgcaatgcg ggcgaccttt gccggggaag aaatgacggg cacaaaaatg cgcgccttga
     2821 tgccgaggac actggcagca taggcaacag ccgcgccatg attgccgcca gaggccgctg
     2881 ccacgccggc tccgggaatt ttcacaccga caaggttggt gaatgcgccg cgcggcttga
     2941 agctgccagt gtgctggaaa agctcaagct tgaggcaaac cggattttca attccaggca
     3001 gggtgatatc catgaccggg gtccggcgga tgaagggcgc aatgcgggta gcagcttcgc
     3061 tgatggtggc gcgggtgaca gtcatgacgg gctatgcagg gctccgatga tgtcgaggca
     3121 gggctggccg aggctccgtg aaatcgcgga ttcgagaatg cccgaggaga gcttcagcgc
     3181 ttctggcggt gcaaagccat tgaggcggga tgcaaagtag aggcccgcca gaaaatcgcc
     3241 ggtgccatga ggcacttcga ccttcacagt actgattgtt tccgcatagt cgcttccggt
     3301 gattgcaagc gttgcaaggt taccgtgacc cacgggaata gacgttgcaa gaatttcctt
     3361 gtgcggaagc gtcgc
//
LOCUS       DAORVN010000158         3372 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_23842_length_3372_cov_3.507085, whole genome shotgun sequence.
ACCESSION   DAORVN010000158 DAORVN010000000
VERSION     DAORVN010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3372
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23842_length_3372_cov_3.507085"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..998)
                     /gene="prfB"
                     /locus_tag="PKV23_04210"
                     /pseudo
     CDS             complement(1..998)
                     /gene="prfB"
                     /locus_tag="PKV23_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_111199041.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
     gene            complement(1032..>3372)
                     /locus_tag="PKV23_04215"
     CDS             complement(1032..>3372)
                     /locus_tag="PKV23_04215"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HQX84674.1"
                     /translation="DGSLLAEYANERRLFIPINSVPKRLIQAYISAEDKTFFTHGGLD
                     WRGIAAAGLRYFQVKLTGKGQIVGASTITQQVAKNFLLSNEQTIERKLREAIIVQRIE
                     AAFTKDQIIELYLNDIFLGLNSYGIAAASLNYFGKSLTELSLDEMAYLAALPKGPNNY
                     HPFRHKDRAIERRNWVLQQMLENDYITQAEFDDATKKPLVVSPRPFGAQLFAAESFAE
                     EVRRELAQMYGKDTLGKGGLSVRTTLDPKLQIYARQALARGLIAFDRRRGFRGPVKKV
                     EVQGDWGLLLAKPRVPADVAPWRLAVVTEVNEQQATIGLQPKLLPDGKLEEARETGTV
                     PAALLGWARAYVDGTELGPKVEKATEVLAVGDVVYVAPSSQEGQWHLVQMPDVEGALV
                     AMDPHTGRVLSMVGGFSYGNSQFNRAVQAMRQPGSAFKPVAYAAALDNGYTPSSVVLD
                     AAVEYQLPNGEVWKPKNYQDKFFGPSTLRRGIEQSRNVMTVRLADDLGMTKIVDLAQR
                     LGLYDKMPLQLSMALGAGETTLMKMTTAYSIFANGGKKVQATLIDRVQDRYGRTIFRY
                     DKRDCSFCQQESYTANSAEPELIDVREQVMSPYTAYQITSMMEGVVQRGTGKKLQIVG
                     KPVAGKTGTSNEEKDAWFIGYTPDLAVGVYVGYDTPKPMGKRRTGGELAAPIVADFMR
                     LALREQPATPFRVPRAIELIPIDANTGQRAIYGEEGVILEAFKPGEEPAGSTTIIGED
                     LASVTTESIYGAGQQLVPPADSEQSVEEGGLTTGTGGLY"
BASE COUNT          645 a          973 c         1008 g          746 t
ORIGIN      
        1 tgaccaactg atagggttgc agaacatatg agcggatctg gtggccccag ccaatatcgg
       61 ttttcttggc gttgactgcg tcgatcttct cctgctgcat ctgcaattcg cgctcgtaaa
      121 gacgagcctt cagcattttc caggcggtgg cccggttctt gtgctgggac cgctcggcct
      181 ggcaggccac gacaatgccg gtgggtaaat gagtaatgcg caccgccgag tcagttgtat
      241 tgacgtgctg gccgccgccc cccgaggcgc ggtaggtgtc gatcttgcat tcactctcgt
      301 tgacctggat atcgatattg tcgtcgatta ccggataggc ccagacggag gcgaaagagg
      361 tgtggcgcct ggcgttcgaa tcatagggcg agatgcggac cagacgatgt acgccggatt
      421 cggttttcaa ttttccatat gcattgtggc ccttgatttc gagagtgcat gacttgatgc
      481 ccgcctcttc cccggcgtgc tcgtcgagca cggagacctt gaacttgttc tggttggccc
      541 agcgcgtata catgcgcatc agcatcgaag cccagtcctg gctttcggtg ccgccagcgc
      601 cggcattgat ctgcaagtag ctgtcgttgc catccgcctc gccggaaaga agtgtttcaa
      661 gttcctgctc ggccacttcc aactggagtt tggtgaggga tttctccgcc tcgctgacga
      721 tggcgtggtc accttctaat tcaccaagtt caatcaggcc cacattgtct tcaatggttt
      781 gctcgaggcg caaaacggat gaaatgcgcg catccaggtc ctggcgcttg cgcatcactt
      841 cctgggcctt ctgcgggtcg ttccaaagat tgggatcttc cgaccgggcg ttcagctcgg
      901 caagctggcg aatcgcggaa tcccagtcaa agatgcctcc tcagcaatcc aatggactgc
      961 ttcatctctt cgacgacttt gacggtttca gcgcgcataa gtgaacagct ttcagattgg
     1021 tttggtgggg ctcaatagag gccgccggtg cctgtcgtca acccgccttc ttccacggac
     1081 tgttcgctat cggcgggcgg cacgagctgc tggccggcgc catagatgga ctcggtcgtg
     1141 acagaggcca aatcctcgcc gatgattgtc gtcgaaccgg cgggctcctc accgggcttg
     1201 aaggcctcga gaatgacgcc ctcctcgcca tagatcgccc gctgtcccgt attggcatca
     1261 atcggaatga gctcaatggc gcgcggtacg cggaagggcg tggccggctg ttcgcggagg
     1321 gccaggcgca tgaaatcggc gacgatggga gcggccaatt cgccgccggt gcgccttttg
     1381 cccatgggct tgggcgtgtc gtagcccaca tacacaccca ccgcgagatc aggcgtatag
     1441 ccaatgaacc acgcgtcctt ttcctcattg gaggtgccgg tctttccggc cacgggcttg
     1501 cccacgattt gaagtttctt gccggttccg cgctggacaa cgccttccat catcgaggta
     1561 atctgatagg ccgtataggg gctcatgacc tgttcacgga cgtcgatgag ttcgggttcg
     1621 gcgctgtttg cggtatagga ttcctgctgg cagaaactgc aatccctttt gtcatatcgg
     1681 aaaatcgtgc ggccgtaacg gtcctgcacg cggtcgatca gggttgcctg aaccttcttg
     1741 ccgccgttcg cgaagatgct gtaggcggtg gtcattttca tcaaggtcgt ttcgcccgcc
     1801 cccagggcca tggacagttg caggggcatc ttgtcgtaga ggcccaggcg ctgggcaagg
     1861 tcaacgatct tggtcatgcc gagatcatcg gcgaggcgca cggtcatcac gttgcgcgat
     1921 tgctcaatac cgcggcgcag cgtcgagggg ccgaaaaact tgtcctgata attctttggc
     1981 ttccacactt cgccattcgg cagctggtat tcaacagcgg catcgagcac gacggacgac
     2041 ggcgtatagc cgttatccag tgctgcggca taagcaaccg gcttaaaggc agaacccggc
     2101 tggcgcatgg cctgaacagc gcggttgaac tggctattgc cgtaggagaa gccgccgacc
     2161 atggaaagca cgcggccagt atgtgggtcc attgcgacaa gggcgccctc cacatcaggc
     2221 atctgcacca gatgccattg gccttcctga ctgcttggag ccacatagac aacgtcgccc
     2281 accgccagaa cttcagttgc cttttcgact ttgggaccga gctccgtacc atccacatag
     2341 gcccgggccc agcccaggag cgctgcggga accgtgccgg tttcgcgcgc ctcttccagc
     2401 ttgccatcgg gcagcagttt tggctgcagg ccaatggttg cctgctgctc attcacctcg
     2461 gtgacaaccg ccagacgcca aggcgccacg tcggcgggaa cccggggctt tgcaaggaga
     2521 agaccccagt cgccctgaac ttcgactttc ttgaccggcc cgcgaaatcc gcgcctgcgg
     2581 tcgaaggcaa tgaggcctct cgccagggcc tgcctggcat agatctgcag ttttggatca
     2641 agggtagtgc gaaccgaaag cccgcccttg ccaagcgtgt ccttgccgta catctgggcc
     2701 agttcgcggc gcacttcctc ggcaaaggat tcagcggcaa agagctgggc gccaaaaggg
     2761 cgcgggctga cgaccagcgg cttcttggtg gcatcgtcaa attcagcttg ggtgatgtag
     2821 tcattttcca gcatctgctg gagcacccag ttgcgccgct cgatggcgcg atccttgtgc
     2881 cggaacgggt gatagttgtt gggacccttg ggcaaggccg ctagataagc catttcatcc
     2941 agggaaagct cggtcagcga cttgccgaaa taattcagcg aggccgcggc aatgccgtag
     3001 gagttcaggc caaggaatat gtcgttcaga taaagctcta taatctgatc cttggtaaag
     3061 gcggcttcaa tacgctggac gatgatggcc tcgcgcagct tgcgttcgat cgtctgctca
     3121 ttgctgagga gaaagttctt ggccacctgc tgggtaatgg tggatgcccc gacgatctgt
     3181 cccttgccgg tcagcttgac ctggaagtag cgcaggccgg cggccgcaat gccgcgccag
     3241 tcaaggccgc cgtgagtgaa aaaggttttg tcttccgcag aaatgtaagc ctggatcagc
     3301 ctcttgggga cggaattgat aggaatgaac aggcgccgct cgtttgcata ttcagcaagc
     3361 agcgaaccat cg
//
LOCUS       DAORVN010000159         3368 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_23913_length_3368_cov_3.220646, whole genome shotgun sequence.
ACCESSION   DAORVN010000159 DAORVN010000000
VERSION     DAORVN010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3368)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3368)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3368
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23913_length_3368_cov_3.220646"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..655)
                     /gene="panB"
                     /locus_tag="PKV23_04220"
     CDS             complement(<1..655)
                     /gene="panB"
                     /locus_tag="PKV23_04220"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011664756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HQX84675.1"
                     /translation="MSVQKMSKRLTAPEISERKGGEPIVALTSYHAHTAAIADKYVDF
                     ILVGDSLGMVMHGFESTLPVPLDLMILHGRAVIRGAKRALVVVDMPFGSYEESHAQAF
                     RNAARVVKETECGAIKVEGGKRMADTIHFLTQRGIPVMAHIGLTPQFINVFGGFKTQG
                     RTRDTWAAIEEDAKAVAEAGAFAVVLEAIVEPLAKKITKSLAIPTIGIGASAACDGQI
                     "
     gene            complement(659..1228)
                     /locus_tag="PKV23_04225"
     CDS             complement(659..1228)
                     /locus_tag="PKV23_04225"
                     /inference="COORDINATES: protein motif:HMM:NF018949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrU family protein"
                     /protein_id="HQX84676.1"
                     /translation="MLILAIGVLIFVLLHGVAAVPRLKSYVKSRTGERWYGPIFGLAS
                     VLAIAVIVLGWRSSDFVALYDPPGWGWYVNYVLTFIAFLCVGVFLFRGGYRQRLRFPM
                     AIATVFWATGHLFANGDLASLILFGGLLVGNTAHMAVAAANGIRPTPEVRIGHNGLSL
                     IFGAALYGIMTQLHPAVIGVPIFAISKAG"
     gene            complement(1228..1800)
                     /locus_tag="PKV23_04230"
     CDS             complement(1228..1800)
                     /locus_tag="PKV23_04230"
                     /inference="COORDINATES: protein motif:HMM:NF018949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrU family protein"
                     /protein_id="HQX84677.1"
                     /translation="MGYLIIGIGFFAGLHAFSMLLPQQRDALKVNLGEGPYKGLYSLI
                     SLAGLALMIWGFWSLTSDPLAGSIVYQPAPWTRHVTMLLVLLGFISLSGFHGKGYMKQ
                     WLRNPFSVGIVLWSVGHLLSNGMLHDVLLFGTFLVLAALDIILSTVRGKRPGHDPQIG
                     SDMKAVVAGVALYAIFLFGVHPYVFNVPIV"
     gene            complement(1801..1998)
                     /locus_tag="PKV23_04235"
     CDS             complement(1801..1998)
                     /locus_tag="PKV23_04235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX84678.1"
                     /translation="MVFELNANTQDLTKTRSLRQRHDGQHHSWQSFSAMITLKMWQTG
                     WDEETGWRGGQNLANTPAGGA"
     gene            2043..2804
                     /locus_tag="PKV23_04240"
     CDS             2043..2804
                     /locus_tag="PKV23_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013912927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX84679.1"
                     /translation="MKPTLILVGADKGGVGKTTVSRALLDFFARKNILTRAFDTENPR
                     GTLKRFYPDLAEIIDLGNVAHQMKVLDTMDTANVPVTIVDLKAGNMSYALDIFERIGV
                     LEAARAGAFNLGLFHVIGPSIASLDEIAEIAHYLQGVHYVVARNFINETNFFEWDQAT
                     QRKYFAQIPKAQEIAIPKLNEMAYEQVDLAGVTFKNFIENRSPDGSPGNYSFVLRGYV
                     RKWSSEIDEQFAHVKLIHDLLTGGRLPQPQLVAQN"
     gene            2822..>3368
                     /locus_tag="PKV23_04245"
     CDS             2822..>3368
                     /locus_tag="PKV23_04245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX84680.1"
                     /translation="MADKPTIYIVCSDRHRNGKTLLARVLVDYLMLEKRDPFVIDAGF
                     PEGPLRAAFPGRTALVDFSQVQGQMKLFDTIMGSPGRDYVIDLPVPQTENFFATYSQL
                     DFHIEATRAGFQIVVFFIVDKDYSSLRFSEDVQRQVAPALFIPVRNIYIGSALVPRGD
                     TLSIDIPELDREVMNLIEARHF"
BASE COUNT          775 a         1044 c          883 g          666 t
ORIGIN      
        1 ggatctgccc gtcgcaggcc gccgatgcgc caatgccgat tgttggtatt gccagcgact
       61 tggtaatctt cttggccaga ggttccacga tggcttcgag cacgacagca aaggccccgg
      121 cctcagccac cgccttcgcg tcctcttcaa tggcggccca ggtgtcacgg gtgcggccct
      181 gggtcttgaa gccgccaaag acattgatga actgcggcgt cagtccgata tgcgccatga
      241 ccggaatgcc gcgctgcgtg aggaaatgga tggtgtctgc catgcgcttg ccgccttcta
      301 ccttgatggc gccgcactcc gtttccttga ccacccgcgc cgcattgcga aaggcctggg
      361 cgtggctttc ctcataggag ccaaagggca tgtcaaccac gaccagcgcc cgtttggccc
      421 cgcgtatcac ggcccggccg tgcaggatca tgaggtcaag cggcaccggc agggttgatt
      481 caaagccatg catcaccatc ccgagcgaat cccccaccag gatgaaatcc acatatttgt
      541 cggcaatcgc cgccgtatgg gcgtgatagg aggtgagcgc cacgatgggc tcgccgcctt
      601 tccgctcgct gatctccggg gccgtcagac gctttgacat tttttgaacg gacatggctt
      661 aacctgcttt tgatattgca aaaatcggaa cgccgatgac ggcgggatga agctgcgtca
      721 tgatcccgta aagcgctgcg ccaaaaatca gcgacaggcc gttatggcca attcgaacct
      781 cgggtgttgg gcgaatgcca ttggccgcgg cgaccgccat atgcgccgtg ttccccacga
      841 gcaagccgcc aaaaaggatc aggcttgcca ggtcgccatt ggcgaagagg tggcccgtgg
      901 cccagaacac cgttgcaatc gccatgggaa atcgcaggcg ttggcggtag cctccgcgaa
      961 acaggaaaac tcccacgcag aggaaggcaa taaaggtgag cacatagttc acataccacc
     1021 cccagcccgg aggatcatat aacgccacaa aatcggaact ccgccagccc agcacaatca
     1081 cggcaattgc aagaactgac gccaggccaa aaatcgggcc ataccaccgc tctcctgtcc
     1141 gcgatttgac gtagctctta aggcgcggga cagccgcaac cccatgtaga agcacaaaaa
     1201 tcagcacacc aattgccagg ataagcatca gacgatcggc acattgaaga cataggggtg
     1261 aaccccgaac aggaagatgg cgtaaagcgc caccccggca accacggcct tcatatcgga
     1321 accgatttgc gggtcatggc ccgggcgctt gccacgcacg gtggagagaa tgatgtccag
     1381 agccgccaaa accagaaacg tgccaaacag cagcacgtcg tgcagcatgc cgttcgacag
     1441 cagatggccg actgaccaaa gcacaatgcc aacggagaag gggttgcgca gccattgctt
     1501 catgtatccc ttgccatgaa acccgctcag cgaaataaaa ccaagcaata ccaaaagcat
     1561 ggtcacatgg cgcgtccagg gcgccggttg atagacaatg ctgcccgcaa gcggatccga
     1621 ggtcaggctc cagaaacccc agatcatcag ggcaaggcca gccagtgaaa tcagcgaata
     1681 gagtcccttg tacggccctt cacccaaatt cactttcaaa gcatcacgct gttgcggcag
     1741 aagcatggaa aatgcatgca ggcctgcaaa aaacccaatg ccgataatca ggtaacccat
     1801 tcaagctcct cctgcgggag tgtttgccag attttggccg ccgcgccaac cggtctcctc
     1861 atcccaccct gtttgccaca ttttaagcgt gatcattgct gaaaatgact gccaactgtg
     1921 atgctgccca tcatgccgct gacgaaggct tcgtgttttc gtcaggtctt gagtgtttgc
     1981 gttcagttcg aaaaccatga taacaattcc tcgataaaga ggccaaatgc ggagggacta
     2041 ccatgaaacc aacactcatt ctcgtgggcg cggacaaggg cggcgtgggc aaaaccaccg
     2101 tatcgcgcgc cttgctggac ttttttgccc gtaagaacat cctcacccgg gctttcgaca
     2161 cggaaaaccc ccgcggcacc ctgaagcgct tttatcccga tcttgccgaa atcatcgatc
     2221 tcggcaatgt cgcccaccag atgaaagtgc tggacaccat ggacacggcc aatgtgccgg
     2281 tcaccatcgt cgatctcaag gccggcaaca tgtcatacgc cctcgacatc tttgagcgca
     2341 ttggcgtcct cgaagccgcc cgcgccggcg ccttcaacct tggcctgttc catgtcatcg
     2401 ggccgtccat tgcctcgctc gatgaaatcg ccgaaatcgc ccactacctg caaggcgtgc
     2461 attatgtggt cgcccgcaat ttcatcaatg aaaccaattt ctttgagtgg gatcaggcca
     2521 cccagcgcaa gtacttcgcg cagattccca aggcgcagga aatcgccatc ccgaaactca
     2581 acgagatggc ctatgagcaa gtcgacctcg ccggcgtcac cttcaagaac ttcatcgaaa
     2641 accgctcacc cgatggttcg ccgggcaatt attccttcgt cctgcgcggc tatgtccgca
     2701 agtggagttc cgagatcgac gaacagtttg ctcatgtgaa gttgatccat gatctgctca
     2761 ccgggggccg tttgccgcaa ccgcaactcg tcgcccaaaa ctaattcgaa acggctgaac
     2821 tttggccgac aagccaacga tctacatcgt ctgctccgac cggcaccgca atggaaaaac
     2881 acttctggcg cgcgtgctgg tcgattatct catgctcgaa aagcgcgatc ccttcgtgat
     2941 cgacgcgggc ttcccggaag gcccgctgcg cgccgccttt cccgggcgca ccgcacttgt
     3001 cgatttctcc caggttcagg gccagatgaa attgttcgat acgatcatgg gttcgcccgg
     3061 ccgcgattat gtgatcgatc tgcctgtccc ccagaccgaa aacttttttg caacttacag
     3121 ccagctcgac ttccatatcg aggcaacccg cgccggcttt cagatcgtgg tctttttcat
     3181 cgtcgacaag gattattcat cgctcaggtt ttccgaggac gtccagcgcc aggtggcccc
     3241 ggcgctcttt atccccgtcc ggaatattta cattggcagc gccctggttc cgcgcggcga
     3301 taccctgtcg attgacattc cggagctcga ccgcgaggtc atgaacctca tcgaagcccg
     3361 gcactttt
//
LOCUS       DAORVN010000160         3351 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_24131_length_3351_cov_3.568871, whole genome shotgun sequence.
ACCESSION   DAORVN010000160 DAORVN010000000
VERSION     DAORVN010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3351
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24131_length_3351_cov_3.568871"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="PKV23_04250"
     CDS             <1..252
                     /locus_tag="PKV23_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009463444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HQX84681.1"
                     /translation="QDVARGAGLLAVYSLWLGIQFLLAAAGVGTFLGFSGYFKRHLLT
                     VERVMGVLLVLTGIMFITGSMQIMAYWFLENFPWLATIG"
     gene            complement(239..541)
                     /locus_tag="PKV23_04255"
     CDS             complement(239..541)
                     /locus_tag="PKV23_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019858987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase accessory protein"
                     /protein_id="HQX84682.1"
                     /translation="MNPPDLKDPVFNEPWEAQAFAMVVKLHERGHFTWSEWAETLGAE
                     IKSSDRPYYEHWLAALEKIVEAKGLMSQVERLNRVEAWDRAAHATPHGKPIELSRS"
     gene            complement(528..1127)
                     /locus_tag="PKV23_04260"
     CDS             complement(528..1127)
                     /locus_tag="PKV23_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011158357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase subunit beta"
                     /protein_id="HQX84683.1"
                     /translation="MNGVHDLGGMMGFGPIAASAVEPVFHEKWEGRMAALVMAVPGDW
                     SIDEDRAACESMHPAKYLKTSYYEHWLHGLELLLKKHTPDSASAMRPADIWPALTARR
                     SYVRDIETPAAFAVGQNVRVKVMHPAGHTRAPRYVRGHTGEVVRLHGAHVFPDSNALG
                     RGENPQWLYTVRFTAKELWGRANDDTVMLDLWEPYLEPA"
     gene            complement(1124..1777)
                     /gene="nthB"
                     /locus_tag="PKV23_04265"
     CDS             complement(1124..1777)
                     /gene="nthB"
                     /locus_tag="PKV23_04265"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: protein motif:HMM:TIGR03888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase subunit beta"
                     /protein_id="HQX84684.1"
                     /translation="MNGPQDMGGMMGFGPVKPEINEPVFHEDWERRVLGLTLAMDACG
                     LWNIDILRHTRECFPAEIYWSVPYYDLRIEAHIKVMLARGLVTEAEILAGHMQVPPKP
                     VKAVLKPEKVGPLLAKGSAYIRTAASLPLFKAGDSVRVRNLHAQGHIRLPGYLRGKTG
                     EVVRYHKAHVFPDSNAHGAGENPQHLYTLRFKAREVFGADSDDDLQADLFEPYLEAP"
     gene            complement(1774..2388)
                     /gene="nthA"
                     /locus_tag="PKV23_04270"
     CDS             complement(1774..2388)
                     /gene="nthA"
                     /locus_tag="PKV23_04270"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008876568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase subunit alpha"
                     /protein_id="HQX84685.1"
                     /translation="MPHDGHDHDDHSELSPIALRVKALESLLVEKGYVDPAALDVLID
                     TYETKIGPRNGAKVVAKAWTDPAYAKWLKEDATAAIESLGYTGRQGEHMVALFNTPEI
                     HNMVVCTLCSCYPWPVLGLPPVWYKSPPYRSRAVIDPRGVLAEFGVTLPEKTKVSVWD
                     STAEIRYLVIPMRPANTDGLSEDELAGLVNRDSMIGTRLAGKVA"
     gene            2446..3126
                     /locus_tag="PKV23_04275"
     CDS             2446..3126
                     /locus_tag="PKV23_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009338186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HQX84686.1"
                     /translation="MAGLEGIRACVFDAYGTLFDVGSPVQKLATEIGDKASGLAALWR
                     QKQLEYTWLRSLMGVHADFWHVTGDALDYSLEALKIDDPGLRDELMTLYLKLDAYPEV
                     AAALQAVRAKGKRTAILSNGSPSMLDSAVRAAGLEKSFDMVLSVEDVGIYKPSRRVYR
                     HAMQKLQIQDAPAICFVSANSWDAQAAAQFGFQVVRLARGGGQDDRIPGKVAARIESL
                     SELAALLA"
BASE COUNT          610 a          949 c         1070 g          722 t
ORIGIN      
        1 caggatgtgg cgcgcggcgc cgggctgctc gcggtttatt cgctgtggct tggcattcag
       61 ttcctgctgg ccgccgccgg ggttggaaca tttctcggct tttccggata tttcaaacgg
      121 catctcttga ccgtcgagcg ggtgatgggc gtactgctgg tgctgaccgg catcatgttc
      181 atcaccggct cgatgcagat catggcctac tggttcctgg aaaacttccc gtggctggct
      241 acgatcggct gagttcgatg ggcttgccgt gcggcgtggc atgggcggcg cggtcccagg
      301 cttcgacgcg gttcaggcgc tcgacctgtg acatgaggcc cttggcctcg acgatttttt
      361 caagtgccgc aagccagtgt tcataatagg gtctgtcaga agattttatt tcggcgccca
      421 gggtttcggc ccattcgctc caggtgaaat gcccgcgctc atggagcttc accaccatgg
      481 cgaaggcctg cgcctcccag ggttcattga ataccgggtc cttgagatca ggcgggttca
      541 agatagggct cccagagatc gagcatcacc gtgtcatcat tggcacgacc ccataattcc
      601 ttggcggtga agcggacggt gtaaagccat tgcggatttt cgccgcggcc cagcgcattt
      661 gaatcgggga agacatgggc gccgtggagt cttaccactt cgcccgtatg cccccgcaca
      721 taacgcgggg ctcgcgtgtg gcctgctgga tgcatgactt taacgcgcac gttttggcca
      781 acagcaaatg cggctggtgt ttcaatatct cgcacatagc tgcggcgcgc cgtcagcgcc
      841 ggccagatgt ctgccgggcg cattgcactg gcgctgtcgg gtgtgtgttt cttcagcaga
      901 agttccaggc cgtgcagcca gtgctcgtag tagcttgtct tgagatattt ggcggggtgc
      961 atggattcgc aggccgcccg gtcctcatca atggaccagt cccccggcac ggccattacg
     1021 agggcggcca tgcggccctc ccacttttca tgaaacaccg gttcgactgc agacgcggcg
     1081 atggggccga agcccatcat gccgccaaga tcatggacgc cgttcatggg gcctccagat
     1141 agggctcgaa gagatcggcc tgcaggtcat cgtcagagtc cgcgccaaaa acctcgcgcg
     1201 ccttgaagcg cagggtgtag aggtgctggg gattctcgcc cgcaccatgg gcatttgaat
     1261 cggggaacac atgagccttg tgataacgga caacttcacc agtcttcccg cgaaggtagc
     1321 cgggcagacg gatgtggccc tgcgcgtgaa gattgcggac cctcacgctg tcgcccgcct
     1381 tgaacagtgg caggctggcg gcagttctaa tgtaggcgct gcccttagcg agcagtggtc
     1441 caactttttc gggcttaagc acggctttca cgggtttcgg cggaacctgc atgtggcctg
     1501 ccagaatttc ggcttcggtg acaagcccgc gggcgagcat caccttgata tgggcctcga
     1561 tgcgaagatc atagtagggc actgaccagt aaatttccgc cggaaaacat tcccgcgtgt
     1621 ggcgcaggat gtcgatgttc cacaggccgc aggcatccat ggcaagggtg agccccagca
     1681 cgcggcgctc ccaatcttca tgaaacaccg gctcgttgat ctcgggcttg accgggccaa
     1741 agcccatcat gccgcccatg tcctgggggc cgttcacgcg actttccccg caagcctggt
     1801 gccgatcatc gagtcgcggt tcacaagccc agcgagctca tcttcgctca ggccatcggt
     1861 gttggcgggg cgcatgggga tcacgagata gcggatctcg gcggttgaat cccagacgct
     1921 gactttggtt ttttcgggaa gggtcacgcc gaattcggcg agcacgccgc gcggatcgat
     1981 gacggcgcgc gagcggtagg gcggcgattt gtaccagacg ggcggcaggc ccagcaccgg
     2041 ccacggataa caggagcaga gggtgcagac caccatgttg tggatttccg gcgtgttgaa
     2101 cagcgccacc atgtgttcgc cctggcggcc ggtgtagccc aggctttcaa tcgccgctgt
     2161 cgcatcctcc ttcagccatt tggcgtaggc cggatcggtc caggctttcg ccaccacctt
     2221 ggcgccattg cgcgggccga tcttggtttc gtaggtgtcg atcagaacgt cgagggctgc
     2281 cggatcgaca tagccctttt caacgagcag cgactccagc gccttgacgc gcagggcgat
     2341 gggggaaagt tccgaatggt cgtcgtgatc gtggccgtcg tgtggcatgt catttttccg
     2401 gttgttggtt tagcatagcc ggagattgcg ggacggagaa gggcaatggc tggactggaa
     2461 ggcataaggg cctgcgtttt tgatgcctat ggcacgctgt ttgatgtggg ttcgccggtg
     2521 cagaagctgg caaccgagat tggcgacaag gcctcgggcc ttgctgcgct gtggcggcag
     2581 aagcagctgg aatacacctg gctccgcagc ctcatggggg tgcatgcgga tttctggcat
     2641 gtgaccggcg atgcgctgga ctattcgctg gaggctctga agatcgacga tccgggcctg
     2701 cgcgatgaac tgatgacgct ttacctgaag cttgacgcct atccggaagt tgcggcggcg
     2761 ctgcaggcgg tgcgggccaa gggcaagcgc acggccatac tctccaatgg cagcccctcc
     2821 atgctggaca gcgcggtgcg cgccgcaggc ctcgaaaaat ccttcgacat ggttctatcg
     2881 gtggaggatg tgggaatcta caagccgagc cggcgggtct accgccatgc catgcagaag
     2941 ctgcaaatcc aggacgcacc cgccatctgc ttcgtctccg ccaacagctg ggacgcgcag
     3001 gcggcggcgc agttcgggtt tcaagttgta agactcgcgc gcggcggcgg gcaggacgac
     3061 aggattccgg ggaaggtggc ggcacggata gagtcgctga gcgaacttgc agcgctgctc
     3121 gcataattca ccgctctcat catgaccggg cttaacccgg tcatcttcct atctgccagg
     3181 caggtatcga atcttcctag tcgttccgtt atgttttgaa aattgtgtgg gagggactta
     3241 cgatggaatc agcggataca gtgggtttgg cagattttat aggcttgtta actccatact
     3301 ttattgtcgt ggtgctgacc attatcccgg ccatcagaat cttaagacgt g
//
LOCUS       DAORVN010000161         3337 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_24302_length_3337_cov_3.843693, whole genome shotgun sequence.
ACCESSION   DAORVN010000161 DAORVN010000000
VERSION     DAORVN010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3337
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24302_length_3337_cov_3.843693"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..396)
                     /locus_tag="PKV23_04280"
     CDS             complement(<1..396)
                     /locus_tag="PKV23_04280"
                     /inference="COORDINATES: protein motif:HMM:NF018960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HQX84687.1"
                     /translation="MLVNDSKDASQAYAHIRHPGTRALFSFWEKIRGEKAAPGRDDLD
                     LRQISPIVPNLLILERDHLHQSYKWRLAGSEICQLYRQKLTSTNALAGWGGFERNTLK
                     QLFDTVVTGLQPCLVRFQLSTDLGQVIGAE"
     gene            499..1227
                     /locus_tag="PKV23_04285"
     CDS             499..1227
                     /locus_tag="PKV23_04285"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HQX84688.1"
                     /translation="MSEPDPVKDEPVAREPLFRAPKVVLIVIAILVAVHVAIQLGGQN
                     WQIWSLYAFSFIPARVSGAAPFPGIWGSQAWSFVTYGFLHADMMHLFFNSLWFLVFGS
                     VVARRLGAFRFLVLTCVSTIAGAAAMLFIHWGEAAIMIGASGAVSGLMAAAIPLMYGE
                     GLRLGDTYRMDIATVRPLRPLEILTNRRALIFTLVWIAVTLFSGASGWTGGSFLEEAR
                     IAWEAHLGGFAAGLVAFYWLDRRN"
     gene            1290..1724
                     /locus_tag="PKV23_04290"
     CDS             1290..1724
                     /locus_tag="PKV23_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008598725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HQX84689.1"
                     /translation="MSVSHILGVKGRSVITGTAKETIQSIAQKLSQHRIGAVVIVDEK
                     EAIAGIVSERDIVRALAENGAKSMTMPVSGVMSKAVKTCKAGDSEIELMALMTTHRIR
                     HLPVVDGGKLAGMISIGDIVKFRIEAVEREAEEMKSYIASAG"
     gene            complement(1728..2138)
                     /gene="hisI"
                     /locus_tag="PKV23_04295"
     CDS             complement(1728..2138)
                     /gene="hisI"
                     /locus_tag="PKV23_04295"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018065643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HQX84690.1"
                     /translation="MAASALDETKALHLKFDEHGLIPAIVTDARDGTVLMFAHMNREA
                     LDVTLVTGQIHFYSRSRKLLWKKGETSGETFAALSILTDCDQDVLQIKVTPEGHGAAC
                     HTGRKSCFYRKIVNGELEFIDNEKLFDPDFVYRK"
     gene            complement(2138..2776)
                     /gene="folE"
                     /locus_tag="PKV23_04300"
     CDS             complement(2138..2776)
                     /gene="folE"
                     /locus_tag="PKV23_04300"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007629153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HQX84691.1"
                     /translation="MDAIIKKFQRETSDSKVKRPSQEEAEAAVRVLLAWAGDDPAREG
                     LLDTPRRVTKAFNEYFSGYNEDPAETLSRTFTEVGGYDDIVMLRDIEFTSHCEHHIAP
                     FVGRAHVAYLPTEQVVGISKLARVVEIFARRLQTQETMTAQIANSICASLKPRGAAVM
                     IEAVHTCMSMRGIGKRNVATITTQFTGEFKSNPAMQARFMELVRSPSGGFSL"
     gene            2904..>3337
                     /locus_tag="PKV23_04305"
     CDS             2904..>3337
                     /locus_tag="PKV23_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006612927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HQX84692.1"
                     /translation="MINEIYNRKILEFAADIPGLQRLASPDATAVAHSRLCGSRVTVD
                     LKMKDDVVTDFGQEVKACALGQASSSVMARHVVGSTAAELRKLKEQMYAMLKDGGPAP
                     TGKWADYEAFIPVRDFKARHASTLLTFDAVTDAISQIEAKAEA"
BASE COUNT          656 a          886 c         1051 g          744 t
ORIGIN      
        1 ttcggcgccg atcacttggc cgagatcggt ggagagctgg aaacgcacca gacatggctg
       61 cagtccggtc accacggtat caaacagctg tttcagggta ttgcgctcaa agccgcccca
      121 tccggccaag gcgttggttg aggtaagttt ctggcggtaa agctggcata tttcgctgcc
      181 cgccaggcgc catttatagc tctgatggag gtggtcgcgc tccagaatga gcagatttgg
      241 cacgataggg ctgatctgcc tcaaatcgag atcatcccgg ccgggggccg ccttttcgcc
      301 acggatcttt tcccagaagc tgaacagcgc gcgggtgccg ggatggcgta tatgggcata
      361 ggcttggctt gcgtctttcg agtcgttaac taacattgcg taaccccaag ggctgaggcc
      421 caccgctgcc tgtgccaatc gtgaactttc aggtccagtg ttgcaggggc catgccattc
      481 gacaggagaa atgcattgat gagcgagcca gacccggtga aggatgagcc tgtggcacgg
      541 gagccacttt tccgggcgcc aaaagttgtc ctgattgtga ttgccatcct ggttgcggtg
      601 catgttgcaa ttcaacttgg cggacagaac tggcagatct ggagcctcta tgccttttcg
      661 ttcattccgg cgcgggtctc cggcgctgcc ccctttcctg gcatctgggg atcacaggcc
      721 tggtcgttcg tgacctacgg atttcttcat gccgacatga tgcatctgtt cttcaacagc
      781 ctctggttcc tggttttcgg ttcggtggta gcccgcaggc ttggagcctt caggtttctc
      841 gtgctgacgt gcgtctctac catcgccggt gcggcggcca tgcttttcat ccattggggc
      901 gaagcggcta tcatgatcgg cgcatcgggt gcggtttcgg gcctgatggc agcggccatt
      961 ccgctgatgt atggcgaagg gcttcggctt ggtgatacct accgcatgga tatagcaacc
     1021 gtgcggcccc tgcggcccct ggaaatcctc acgaaccggc gcgcgcttat tttcaccctg
     1081 gtgtggattg cagtcacttt gttttctggc gccagcggct ggacaggcgg ctcattcttg
     1141 gaggaggcgc gtattgcctg ggaagcgcat ctcggcggat ttgcggcagg cttagtggcc
     1201 ttttactggc tggacaggcg gaattgattt accttctgct caggcgtggt acagtgagcc
     1261 tcagaacgtt caagtatcgg gaggtgtgca tgtccgtatc ccatatcctt ggtgtcaaag
     1321 gccgctccgt cataaccggc acggcgaagg aaaccattca aagcatcgcg caaaaactgt
     1381 cgcagcaccg cattggcgcg gtcgtcattg ttgacgaaaa agaagcgatc gccggcatcg
     1441 tttcggaacg tgatattgtg cgggcccttg ccgaaaacgg ggcaaaatcc atgacgatgc
     1501 ctgtctccgg agtgatgagc aaagcggtga agacctgcaa ggccggtgac agtgaaatcg
     1561 aattgatggc gctgatgaca acgcaccgga tccgccatct gccagtggtg gacggaggca
     1621 agcttgccgg catgatttcg atcggcgata tcgtgaagtt ccgcattgaa gcggtcgagc
     1681 gcgaggcgga agagatgaag agctacatcg cttcggcggg ataaggccta tttccggtag
     1741 acgaaatccg gatcaaacag tttctcgttg tcgatgaact cgagctcgcc atttacgatc
     1801 ttgcggtaga agcatgattt gcggcccgta tggcaagcgg cgccatggcc ttccggcgtg
     1861 accttgatct gcaggacgtc ctggtcgcaa tcggtgagaa tgctcagcgc cgcaaaggtc
     1921 tcgcctgacg tctcgccttt tttccagagg agcttgcgcg aacggctgta gaagtggatc
     1981 tggccggtga cgagcgtgac atcgagggct tcgcggttca tgtgggcgaa catgagcacg
     2041 gttccgtcgc gcgcatcagt aacgatggcc gggatcaggc catgctcatc gaacttcagg
     2101 tgaagcgcct tcgtttcatc gagggcggag gcagccatca gagggagaat ccgcccgaag
     2161 gcgagcggac cagttccatg aaccgggcct gcatggccgg gttggacttg aactcgccgg
     2221 tgaactgggt ggtaatggtt gccacattgc gtttgccgat gccgcgcatg gacatgcagg
     2281 tgtgaaccgc ctcgatcatg acggcggcgc cgcggggctt gaggcttgcg caaatggaat
     2341 tggcgatctg ggcggtcatg gtttcctggg tctgcaggcg gcgggcgaaa atctcaacaa
     2401 cgcgggccag cttggaaatg cccaccacct gttcggttgg cagataggcc acgtgggcgc
     2461 ggccgacgaa gggggcgatg tggtgttcgc aatgggacgt gaactcaata tcgcgcagca
     2521 tgacgatatc gtcatagccg cccacctcgg taaaggtgcg cgaaagggtt tcggccgggt
     2581 cctcattata gccgctaaag tactcgttga atgccttggt gacgcggcgc ggcgtatcca
     2641 gcaggccttc acgggccggg tcgtccccgg cccaggcaag aaggacgcgc acggcggcct
     2701 cggcctcttc ctgagacgga cgcttaacct tggaatcgct ggtttcgcgc tggaatttct
     2761 tgatgatagc gtccattttg tctctttgat tttgggtact aaattgactt tactaaagtt
     2821 tctggtcttc ccatatcatg gggggtaaat tgaggcaaac cggcaaagac ccttggttaa
     2881 gcaataccga cgcggatatg tgaatgatta acgagattta caacaggaaa atcctcgagt
     2941 tcgcggcaga cattccgggt ttgcaacgct tggcatcgcc agatgccacc gcggtggccc
     3001 attcccggct ttgcggctca cgggtgacgg tagacctcaa aatgaaggat gacgtggtga
     3061 ccgactttgg ccaggaggtg aaagcctgcg ccctggggca agcctcctcg tcggtcatgg
     3121 cccggcatgt tgttggctcg acggcggcgg agctgcgcaa gctcaaagaa cagatgtatg
     3181 ccatgctgaa agacggcggt ccggcgccca cgggtaaatg ggcggattat gaggctttca
     3241 tcccggtgcg ggatttcaag gcgcggcatg cctcgacgct gctcaccttt gatgcggtta
     3301 ccgatgccat ttcccagatc gaagcgaaag ccgaggc
//
LOCUS       DAORVN010000162         3336 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_24323_length_3336_cov_2.552271, whole genome shotgun sequence.
ACCESSION   DAORVN010000162 DAORVN010000000
VERSION     DAORVN010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3336
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24323_length_3336_cov_2.552271"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="PKV23_04310"
     CDS             complement(<1..300)
                     /locus_tag="PKV23_04310"
                     /inference="COORDINATES: protein motif:HMM:NF012799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HQX84693.1"
                     /translation="MKTGVLYGIGVGPGDPELMTVKAWRIISMAPVVAYLTANSTEST
                     ARDIAKPFLPDDVIELKIDMPMRTEREPAQEAYDRGAAAITEHLKAGRDVAMTEEG"
     gene            complement(297..1502)
                     /gene="cbiE"
                     /locus_tag="PKV23_04315"
     CDS             complement(297..1502)
                     /gene="cbiE"
                     /locus_tag="PKV23_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007067059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046025 - precorrin-6Y
                     C5,15-methyltransferase (decarboxylating) activity
                     [Evidence IEA];
                     GO_process: GO:0046140 - corrin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6y C5,15-methyltransferase
                     (decarboxylating) subunit CbiE"
                     /protein_id="HQX84694.1"
                     /translation="MTKWLSIIGMGEDGFEGLSARAQLALQNADVIVGSERLLGFLPA
                     LKGERLEWPQPFSAVVDQMKPLRGRNTVILATGDPMNYGVARKLLEFIPFAEMSIIPH
                     LSAFSHAAARVGWSIPDCDTLTLHGRDAANIEAFIQPGAKLIVLTADASTIPEVARRL
                     VARGFGKSPITVLENMGGAREAQTCFMADAVPKQRFSDLNTLAVECIAGTNAKIWSRL
                     AGLPDEAFVHDGQLTKREVRAATLAALAPAPDQLLWDVGAGCGSVAIEWMRSTRGCEA
                     LAFEHHEDRLKMIAANADQLGTPRLKIIAGEVPNTLQGKPGPDAAFIGGGVGIPGVFE
                     TAWEHLKSGGRMVANVVTIEGEMHLYDLQEKHGGVLVRLEVSHLTPIGPYRALKPRMA
                     VTQWRAIKP"
     gene            complement(1499..2107)
                     /locus_tag="PKV23_04320"
     CDS             complement(1499..2107)
                     /locus_tag="PKV23_04320"
                     /EC_number="5.4.99.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007198274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016993 - precorrin-8X methylmutase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-8X methylmutase"
                     /protein_id="HQX84695.1"
                     /translation="MMDYLRDPSAIYEKSFAIIRGETDLSCLPVSAKAIAIRMIHACG
                     MTDIVGDLRISEDFVAAATRALAKPILTDAEMLRHGIIDKSIQVMCTLNDPRAREIGI
                     STQTTRSAASVQLWEPHLEGALVVIGNAPTALFALLEMIDAGGPRPAAIAAFPVGFVG
                     AAESKDELVKNPRGIPCATILGRRGGTAMAAACVNGLSKGLT"
     gene            complement(2104..3105)
                     /locus_tag="PKV23_04325"
     CDS             complement(2104..3105)
                     /locus_tag="PKV23_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009757575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sirohydrochlorin chelatase"
                     /protein_id="HQX84696.1"
                     /translation="MTKLGVMLCGHGSRDTDAVAEFAVLSEHLKKRLPQYPVEYGYLE
                     FATPIIRSGLDVLKAKGVERVLAVPGMLFAAGHAKNDIPSVLNTYAASNGLKIDYGKE
                     LGIDPKMMRAAGDRIQEALDANPSKVPLHETLLVVVGRGASDPDANSNVSKVTRILWE
                     GFGFGWAETAYSGVTFPLVDPGLEHAARLGYKRIIVFPYFLFTGILVQRIYDATDDVA
                     KRHPGIEFIKAPYLNDHPAVIDTFVERVQQILTGDIAMNCQMCKYRTAVLGFEADVGA
                     VQESHHHHVEGINAKGPFEIVNHAHHHGHDHSHDDGHTHAPYPHASHPLGPVSMKKK"
BASE COUNT          617 a          929 c         1118 g          672 t
ORIGIN      
        1 gccttcttcc gtcatggcca catcgcggcc ggccttgagg tgttcagtga tggcggcagc
       61 gcccctgtca taggcttcct gggccggttc gcgctcggtg cgcatgggca tgtcgatctt
      121 gagttcgatc acgtcatcgg gcaggaaggg cttggcgatg tcgcgcgccg tgctttcggt
      181 tgaattggcg gtgagatagg cgacgacagg cgccatggaa atgatgcgcc aggctttcac
      241 cgtcatcagt tcgggatcgc cggggccaac gcctatgcca taaaggactc cggttttcat
      301 ggcttgatgg ctctccactg agtgacagcc atgcgcggct tcaaagcgcg gtaggggccg
      361 atgggcgtga ggtgcgaaac ttcaaggcgc accagaacgc cgccgtgttt ttcctgcagg
      421 tcgtagagat gcatttcgcc ttcgatggtg acgacattgg ccaccatgcg gccgccggat
      481 ttcaaatgct cccaggcggt ttcgaaaacc ccgggaatgc cgacgccgcc cccaatgaac
      541 gcagcatcgg ggccgggttt gccctgcaag gtatttggga cttcgcctgc aatgattttc
      601 aggcgcggcg tgcccagttg atcggcattg gccgcgatca ttttcaggcg gtcctcgtgg
      661 tgttcaaagg ccagggcttc gcagccgcgg gtggagcgca tccattcgat ggcgaccgag
      721 ccgcagccag cgccaacatc ccagagcaat tggtcggggg cgggagccaa tgcggcaagg
      781 gtggcggcgc ggacttcacg cttggtgagc tggccgtcat ggacaaaggc ctcgtcggga
      841 aggccggcca gccgcgacca gatttttgcg tttgttccgg cgatgcattc cacggcgagc
      901 gtattgagat cggagaaccg ctgcttcggc acggcgtcag ccatgaaaca ggtttgtgct
      961 tcgcgtgcgc cgcccatgtt ttccagaacg gtgatcgggc ttttcccaaa gccccgcgcc
     1021 accaggcgcc gcgccacttc cggaatggtg ctggcatcgg cggtcagcac aatcagtttt
     1081 gcgccgggct ggatgaaggc ctcgatattg gcggcatcgc gcccgtgcag agtgagcgtg
     1141 tcgcaatcgg ggatggacca gccgacgcgg gcggcggcat gggagaaggc cgagagatgg
     1201 ggaatgatgc tcatttccgc gaagggaatg aactcgagca gcttgcgcgc cacgccgtaa
     1261 ttcatgggat cgccggtggc gaggatcacg gtgttgcggc cacgcagggg tttcatttga
     1321 tcgaccacgg ccgaaaaagg ctgcggccat tccagccgct cgcctttcaa ggccgggaga
     1381 aagccgagga gacgttcaga acccacgatt acatcggcat tctgcaacgc aagttgtgcc
     1441 cgggcggaca ggccttcaaa gccatcctcg cccatgccga tgatggagag ccatttggtc
     1501 atgtgaggcc tttcgacagg ccgttgacgc aggccgccgc catggcggtg ccgccgcggc
     1561 ggccgaggat cgtggcgcag ggaatgccac gtgggttctt tacgagttca tccttcgact
     1621 ccgccgcgcc gacaaagccc acggggaagg cggcaatggc ggcgggcctt gggccgccgg
     1681 catcgatcat ttcgagaagg gcaaagagcg cagtgggggc attgcctatt accacgaggg
     1741 cgccttccag atggggttcc cacaattgaa ccgaggcggc ggaccgcgtg gtctgcgttg
     1801 agatgccgat ttcgcgggcg cgggggtcat tcagggtgca catgacctga atcgatttat
     1861 cgatgatccc gtggcgaagc atttcggcat cggtcaggat cggttttgcc agggcccttg
     1921 ttgccgccgc aacaaaatcc tcagaaatgc gcaggtcgcc cacgatgtcg gtcatgccgc
     1981 aggcgtggat catgcggatg gcgatggctt tcgcactcac gggcaaacaa gaaagatcgg
     2041 tttcgccgcg aatgatggcg aaggattttt catagatggc cgatgggtcg cggaggtaat
     2101 ccatcatttc ttcttcatgc tgacggggcc aagcgggtgg ctggcatggg gatagggcgc
     2161 atgggtgtgg ccatcgtcat gggaatgatc atggccgtga tgatgcgcgt ggttcacgat
     2221 ctcgaagggg cccttggcgt tgatgccttc cacatggtga tggtggcttt cctgcacggc
     2281 gcccacatcg gcctcgaagc ccagcaccgc cgtacggtat ttgcacatct ggcaattcat
     2341 ggcgatgtcg ccggtgagga tctgctggac gcgctcgacg aaggtgtcga tcacggcggg
     2401 atggtcgttg agatagggcg ccttgatgaa ttcgataccg gggtggcgct ttgccacgtc
     2461 gtctgtcgca tcatagatgc gctggacgag aatgccggtg aagaggaaat aagggaagac
     2521 gatgatgcgc ttgtagccca gccgtgcggc atgttccagg ccggggtcca ccagcgggaa
     2581 ggtgacgccg gagtaggcgg tttcggccca gccgaagccg aagccttccc agagaatgcg
     2641 ggtgactttc gagacattgg agttggcgtc gggatcggag gcgccgcggc ctacgacgac
     2701 aagcagggtt tcatgcagcg gcaccttcga cgggttggcg tcgagggctt cctgtatgcg
     2761 atcgccggcg gcgcgcatca tcttggggtc aatgcccagt tccttgccgt aatcgatttt
     2821 caggccgttg ctggcggcat aggtgttgag cacggaggga atgtcgttct tggcgtggcc
     2881 cgccgcgaaa agcatgccgg gcacggccag cacccgctcc acgcccttgg ctttcaaaac
     2941 gtcaagacca ctgcggatga tgggcgttgc aaattcgagg tagccgtatt ccaccggata
     3001 ttgcggcagg cgcttcttga ggtgctcgga gagcacggca aattcggcga ccgcatcggt
     3061 atcccggctg ccatggccac acagcattac acccagtttc gtcattttgc ctgccttcca
     3121 taaatcagcg ctgccggtca cggctacgga aggcagctcc ggctttggcc ccttcttggg
     3181 ttggccgcac cggaatggct ttcagtccct cgaggggaac gctgctgatg gccccttcat
     3241 aggcgcaagc cggattctgg acaaggatgg aaacggcagc ctatggtgcg ctcacggcat
     3301 ggcaggtttt ggagattccg caatggaaac ggttaa
//
LOCUS       DAORVN010000163         3334 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_24343_length_3334_cov_3.223239, whole genome shotgun sequence.
ACCESSION   DAORVN010000163 DAORVN010000000
VERSION     DAORVN010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3334
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24343_length_3334_cov_3.223239"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..549)
                     /gene="rpsB"
                     /locus_tag="PKV23_04330"
     CDS             complement(<1..549)
                     /gene="rpsB"
                     /locus_tag="PKV23_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006205162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HQX84697.1"
                     /translation="MAVPEFTMRQLLEAGVHFGHQTHRWNPKMQSYIYGSRNGIHIID
                     LAQTVPLLHQALLAVTAAVAKGGRVLFVGTKRTAQEQIAESARRSAQYYVNSRWLGGM
                     LTNWKTISNSIKHLRTLDEQLAKEGMGLTKREMLSISREKAKLDKALGGIKDMGGTPD
                     ILFVIDTNKEALAIKEANRLGIP"
     gene            complement(805..1464)
                     /locus_tag="PKV23_04335"
     CDS             complement(805..1464)
                     /locus_tag="PKV23_04335"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQX84698.1"
                     /translation="MTGKTLGLEGPLYDYYAAHAYREPDILKELRLETAKLGGEARMQ
                     IGPEQGAFMAMLVKLMGAKRILEIGTFTGYSSLAMALAGDAKIVAADVSEEWTKVARN
                     FWKKAGVEGRIDLRLGPGAETIAQLLKVGEAGRFDMMFIDADKSSYDTYYEGGLKLLR
                     TGGVMLIDNVLWSGDVAKPSVKDEDTVALRALNEKIMADERVDLVMVPICDGLTMARK
                     K"
     gene            complement(1472..2008)
                     /locus_tag="PKV23_04340"
     CDS             complement(1472..2008)
                     /locus_tag="PKV23_04340"
                     /inference="COORDINATES: protein motif:HMM:NF020909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1993 domain-containing protein"
                     /protein_id="HQX84699.1"
                     /translation="MTKAAQAETFHRLTIAAMIGSLENLDRMLVKAQSHATKNKIEAG
                     VLLAARLYPDMFSLIQQLQYALYLPMDFARHFTDEPAPHVGYEEATFEDVHASLKTAI
                     AYLKAIPADRLSERAQRVVPIFFDGNKGMPATDYAASVEMPDFYFHLSIAYAILRHNG
                     VPLSKGDFIGPYESIAIE"
     gene            complement(2028..>3334)
                     /locus_tag="PKV23_04345"
     CDS             complement(2028..>3334)
                     /locus_tag="PKV23_04345"
                     /inference="COORDINATES: protein motif:HMM:NF021606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HQX84700.1"
                     /translation="LGLAGVFKKSPPITWGILAAGSALLFLFGAWGRVDGLLSDTSWA
                     LLGALAAAVLLAAVWLRRGKITANEDNFTAGILAIGSAGLLLFAEDRMLNGVWLTISM
                     AVLASAYAFSTRTLAVKLLGPIAAALGSLTALRLFVSRELWNDDRTLPLGQHWPFYGY
                     GIPVVLFYFAARWLKSSGHTKSATALEGISLGLAISLVSLELRVLIGGGLTTDEPQLL
                     EVAAHILTWLGAAYGLMYRQQLFSSLISLWGARILIGASLAAIVFVSLGSLNPIVTED
                     PVPGNVVFNALLLAYLAPVVLLGFILRRLEALGWLQFRPAVGILALVLAMTYVTLETK
                     RVFQGHVLVAWSLSGAESYAYSAVWLVSALALFAAGIKLGRQYIRYAGLAVMVLVVLK
                     VFLWDMSGLEGLYQIGSFLGLGLSLVGIGWLYQRFVQKPKEA"
BASE COUNT          739 a          914 c         1015 g          666 t
ORIGIN      
        1 cgggatgccc agtcgattgg cttccttgat ggcgagggct tccttgttgg tgtcgatcac
       61 gaacaggatg tcaggcgttc cgcccatgtc cttgatgccg cccagggcct tgtcgagctt
      121 ggccttctcg cgggaaatgg agagcatttc gcgcttggtg aggcccatgc cttccttggc
      181 aagctgctca tcgagggtac ggagatgctt gatggaattc gaaatcgttt tccaattggt
      241 gagcatgccg ccgagccagc gggaattcac atagtactgg gccgagcgcc ttgcgctttc
      301 ggcaatctgt tcctgggcgg tgcgcttggt gccaacgaag agcacgcggc cacccttggc
      361 tacggccgcg gtgacggcaa gaagcgcctg atgcagcaat ggcacagtct gggccagatc
      421 gatgatgtgg atgccattgc gcgagccata gatatacgac tgcatcttgg ggttccagcg
      481 atgggtttga tgtccgaaat gtacgcctgc ctcaagcaat tggcgcatag tgaattctgg
      541 cacagccatg gtcttttcct tctccggttg atccgccgcg gatggttgtg accggattat
      601 ccggcaccgg aaggacttca gcctatgagg ggaatttgca ttcccatggc ccaagtccaa
      661 cacccgcgtg agtatttaac gagcggggct atacaggtgc ggtgcagtaa aggcaagcgc
      721 gatggtaaac catgtaaaaa caatggtttg cactgctacc ttgggcgaca cacgcaactg
      781 cctttgcggg ctgttcaggg ggccctactt tttccgcgcc atggtcaggc catcgcagat
      841 tggcaccatc acgagatcaa cgcgttcgtc ggccattatc ttctcattga gggcgcgcag
      901 ggcaaccgtg tcttcatcct tgaccgaagg cttggccaca tcaccgctcc acaggacgtt
      961 atcaatcagc atgacgccgc cggtgcgcag caatttcagg ccgccttcgt aataggtgtc
     1021 gtaggatgat ttatcggcat caatgaacat catgtcgaaa cgcccggcct cacccacttt
     1081 gaggagctgt gcgatggttt cagcacccgg tcctaaacgc aggtcaatgc ggccctcgac
     1141 gccggccttc ttccagaaat tgcgggccac cttggtccac tcttcgctta catccgcggc
     1201 gacgattttg gcatcaccgg ccagggccat ggcgagcgat gagtagccgg tgaaggtgcc
     1261 gatctcgaga atgcgtttgg cgcccatgag cttgaccagc atggccatga aggcgccctg
     1321 ctccggcccg atctgcatgc gtgcctcgcc gccgagcttt gcggtttcaa ggcggagctc
     1381 cttcaggatg tccggctcgc ggtaggcgtg ggcggcgtaa tagtcataga gggggccttc
     1441 aaggccaagg gtttttccag tcattcaaca ctcactctat ggctatggat tcatatggcc
     1501 cgatgaaatc gcccttcgaa aggggcacac cgttgtgacg gaggatggca taggcgatgc
     1561 tcaggtgaaa atagaaatcc ggcatctcga cactggcggc gtagtccgtg gccggcatgc
     1621 ccttgttgcc gtcaaaaaag ataggcacga cgcgctgggc gcgttcggaa aggcggtcag
     1681 ccggtatcgc cttgagatag gcgattgcgg ttttcagact ggcatgcaca tcttcgaaag
     1741 tggcttcttc ataacccaca tgcggcgcgg gctcgtcagt gaagtgccgg gcgaaatcca
     1801 tgggcagata gagggcatac tgcaattgct ggatgaggct gaacatatcg gggtagagcc
     1861 ttgcagccag caatacgcca gcttcaattt tgttcttggt ggcatgggat tgcgccttga
     1921 cgagcatcct gtcgagattt tccagtgaac cgatcatcgc ggcgatggtg aggcgatgaa
     1981 aagtctcggc ctgcgctgcc ttggtcatgt ttttctccct tggggtttca tgcctccttc
     2041 ggtttttgca caaatcgctg atagagccaa cctatgccca caagactgag gcccaagcca
     2101 aggaaggagc cgatctggta gagcccttcc agtcccgaca tatcccagag gaagactttc
     2161 aggaccacca gaaccatgac agctaggccg gcatagcgga tgtattggcg gccaagtttg
     2221 atcccggcgg caaacagggc aagggcggag accagccata cggcggaata ggcgtaggat
     2281 tcagccccgg acaatgacca cgcaaccagc acatgcccct ggaacacgcg cttggtttcg
     2341 agagtcacgt aggtcatggc gaggaccaga gccagaatgc cgactgcggg ccgaaactgg
     2401 agccagccca aggcctccaa acgacgcaag atgaagccga gcaacaccac aggcgcgagg
     2461 tatgccagca gcagggcgtt gaacacgaca ttgccaggga ccggatcttc agtgacaatc
     2521 gggttcaagg acccgaggct cacaaagacg attgccgcca gggatgcgcc aatcaggatt
     2581 cgcgctcccc atagggaaat cagcgaggag aatagctgct ggcgatacat gaggccatag
     2641 gctgcaccga gccaggtgag aatatgggcc gccacttcaa ggagttgcgg ttcatctgtg
     2701 gtcaagccgc cgccgatgag cacgcgaagt tcaagcgaga cgagcgagat ggcaaggccg
     2761 aggctgatgc cttcaagggc tgtggcggat ttggtgtggc cggatgattt cagccagcgc
     2821 gcggcgaagt agaacagaac aacgggtatg ccgtagccat aaaacggcca gtgctggcct
     2881 aggggcaagg tgcggtcatc gttccaaagt tcacgcgaaa caaacaggcg cagggcggtg
     2941 agggagccaa gcgcggccgc aattggcccc agcaatttca cggcaagagt tctggtggaa
     3001 aatgcatagg ctgaggcgag aaccgccatg cttatggtca gccatacgcc attgagcata
     3061 cggtcttcag caaacaggag aaggccggcg ctgccgatgg cgagaattcc ggcggtgaaa
     3121 ttgtcctcgt tcgcggtgat ttttccgcgc cggagccaga ctgctgcaag cagcacagcc
     3181 gcagccaatg caccaaggag agcccatgac gtatcggaca gcaagccatc caccctgccc
     3241 caggcgccga atagaaataa cagcgcggaa cccgctgcca ggatgcccca ggttatgggc
     3301 ggtgattttt tgaaaacacc ggcaaggccc agtg
//
LOCUS       DAORVN010000164         3330 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_24379_length_3330_cov_2.682748, whole genome shotgun sequence.
ACCESSION   DAORVN010000164 DAORVN010000000
VERSION     DAORVN010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3330
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24379_length_3330_cov_2.682748"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..732)
                     /gene="cas7c"
                     /locus_tag="PKV23_04350"
     CDS             complement(<1..732)
                     /gene="cas7c"
                     /locus_tag="PKV23_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas7/Csd2"
                     /protein_id="HQX84701.1"
                     /translation="MSLTNRYDFVLVFDVRDGNPNGDPDAGNMPRLDAESGHGLVTDV
                     SLKRKVRNFIGMMKGETPPFDIYVKEKAVLNQAHEKAYVAIGAEDELKGDAKKRKGSG
                     ETVDKARQWMCKNFFDVRTFGAVMSLGVNCGQVRGPVQLTFARSVDPIIAQEHSITRM
                     AVATEAEAEKQQGDNRTMGRKHTVPYGIYVAHGFVSSFLAKQTGFDENDLELLWQALE
                     NMFEHDRSAARGEMSTRGLYVFKHDS"
     gene            complement(729..2471)
                     /gene="cas8c"
                     /locus_tag="PKV23_04355"
     CDS             complement(729..2471)
                     /gene="cas8c"
                     /locus_tag="PKV23_04355"
                     /inference="COORDINATES: protein motif:HMM:TIGR01863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas8c/Csd1"
                     /protein_id="HQX84702.1"
                     /translation="MILRALYDYYQRKALDPDPARRLPAFGLEEKEIPFVIELAANGR
                     PLLISDTRAGEGKKKLPRRYLVPQGVKRASGIAANFLWDTAEYALGVDSKGKPERVAA
                     QHAAFRKRIADLPESCRQDAGLLALEAFFAGHGQAALADDPLWPEIMEINPILTFRLS
                     GENDLICQRPAIVGAMPQEAAEEGGNAICLVTGQAATAERLHTAIKGVWGAQTSGANI
                     VSFNLDAFTSFNKSQGANAPVSPAAAFAYTTALNHLLERGSRQRIQVGDASTVFWAQK
                     DDPLEEGFAAIFGDQDDPDARADQIRALLSSVDAGVFDGGRGNHRFYVLGLAPNAARI
                     AIRFWYAEPLSVIAHRIRQWFDDLKLHSAPNDPEYPSLFRLLVACAVLNKADNIPPNL
                     GGDVMRAILSGASFPASWLNAAVLRCRAEQNVSYMRAATIKACLNRKLRLTSNQSQEK
                     ELLPMLDPQNISPAYRLGRLFAALEKIQEEASPGLNATIRDRYYGAASSTPVSVFTTL
                     LRLKNHHLGKLSNRGRAVNLERLIGEIMDGLLDFPKHLTLPEQGRFALGYYHQRQAFF
                     TKSESIESTQGESK"
     gene            complement(2468..3121)
                     /gene="cas5c"
                     /locus_tag="PKV23_04360"
     CDS             complement(2468..3121)
                     /gene="cas5c"
                     /locus_tag="PKV23_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003762769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas5c"
                     /protein_id="HQX84703.1"
                     /translation="MDNFCLEVSGDYACFTRPEMKVERVSYDVITPSAARSIFEAILW
                     KPAIRWEVKRIEVLKPIRWISVRRNEVSSKLSVRNAQAAMGAGKGNLALYIEEERQQR
                     ASLLLRDVAYRLHADLVPVGNDALSNPGKYRDMFQRRAEKGQCVNQPYLGCREFASRF
                     RLVSEIDLEASPLNESHDLGWMLYDMDFEHSDDPKPRFFRAQLENGIIDLSKAQVMG"
     gene            complement(3114..>3330)
                     /locus_tag="PKV23_04365"
     CDS             complement(3114..>3330)
                     /locus_tag="PKV23_04365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX84704.1"
                     /translation="IKTVSWPRAAIMNLLGLIRELRLMKDQPSHRFYAVAGLVLFVFI
                     GGVLLRSAFVAGLLMSFGKCLQGGCLG"
BASE COUNT          734 a          906 c          946 g          744 t
ORIGIN      
        1 ggagtcgtgc ttaaagacgt agaggccacg ggtggacatt tcccctctgg ccgcagaacg
       61 gtcatgctca aacatgtttt ccagcgcctg ccagagtagt tcgaggtcgt tctcgtcaaa
      121 gccggtttgc ttggcaagga aggatgaaac gaagccgtgg gcgacgtaaa tgccatacgg
      181 aacggtgtgc ttgcggccca tggttcggtt gtcgccttgc tgtttctcgg cttccgcttc
      241 agtggcaaca gccatacggg tgatggagtg ttcctgggca atgattgggt cgactgagcg
      301 ggcgaaggtc agttgcactg ggccacggac ttggccgcag ttgacgccaa gtgacatgac
      361 agcgccaaaa gtacgtacgt caaaaaagtt tttgcacatc cattggcgtg ccttgtcgac
      421 tgtttccccg ctacctttgc gctttttggc atcacctttc agctcatctt cagcaccaat
      481 agcaacatag gctttttcgt gagcttggtt gaggactgct ttctctttga catagatatc
      541 gaatggtggc gtttcacctt tcatcatgcc gataaagttg cgtaccttgc gcttcaaaga
      601 aacatcagtg acaagcccgt gaccagattc ggcgtcaagc cggggcatat tgccggcgtc
      661 tggatcacca tttggatttc catcgcgaac atcgaaaaca agaacaaaat cgtagcggtt
      721 ggtaaggctc atttggattc tccctgggtg gattcaatag attcggattt ggtgaaaaag
      781 gcttgtcgct gatggtagta gccgagcgca aaacggcctt gctccggcag agtcaggtgt
      841 ttggggaaat caagtagccc atccatgatt tcgccgatca ggcgttccag atttaccgcc
      901 ctgccacgat tactgagctt gccaagatgg tggtttttca ggcggagcag ggtggtgaaa
      961 acgctgactg gcgtgctgga ggcggcgccg taatagcggt cacgaatggt ggcattcagg
     1021 ccgggactgg cttcttcctg gattttctcc agcgcggcaa acaagcgccc aaggcggtat
     1081 gccgggctga tattttgcgg gtcgagcatg ggtaacaact ccttctcttg agattgattg
     1141 ctggttaaac gcagcttgcg attcaggcag gctttgatcg tcgctgcacg catatagctg
     1201 acgttttgct cggccctaca gcgcagcacg gctgcgttca gccatgaggc tggaaatgag
     1261 gcaccggaca ggatggcgcg catgacgtcg ccgccaagat tgggtggaat gttgtcggcc
     1321 ttgttcagca ccgcacaggc gaccagcaac cggaacaggc tggggtattc gggatcattc
     1381 ggtgcgctgt gaagtttcag atcatcaaac cattgccgaa tgcggtgggc gatgaccgaa
     1441 agcggctcgg cgtaccaaaa gcggatagca atccgggcgg cattgggggc cagaccgagt
     1501 acgtaaaacc ggtggttgcc tcgcccacca tcaaatacac cggcatcaac cgaactgagc
     1561 agggcgcgga tctgatcggc gcgagcatcg gggtcgtcct gatcgccaaa gatggcggca
     1621 aaaccttctt cgagcgggtc gtccttttgt gcccagaata cggtggaggc atcgccaacc
     1681 tgtatgcgtt ggcgtgaccc tctttccagc aggtggttaa gggctgtcgt gtaggcaaag
     1741 gcggcggctg gactgactgg tgcgttggcg ccctggctct tgttgaagga ggtgaaagca
     1801 tccaggttga aggagacgat atttgcccca gaggtctgcg ctccccagac gcctttgatt
     1861 gccgtatgca gccgctccgc agtggcggct tggccggtca caaggcaaat cgcattgccg
     1921 ccttcttcgg cggcttcttg aggcatggcg ccaacgatgg cggggcgttg gcagatcaga
     1981 tcgttttctc ctgatagtct gaacgtcagg atggggttga tctccatgat ctccggccaa
     2041 agcgggtcat cagccaaagc tgcttggcca tggccggcaa agaaagcttc cagcgccaat
     2101 agcccggcat cttgccgaca gctttccggc aagtctgcaa tgcgcttgcg gaaagcggca
     2161 tgttgcgcgg caacccgctc cggcttaccc ttgctatcga cccccaaagc gtattcggcg
     2221 gtatcccaca gaaaattggc ggcaatgccc gaggctcgtt tgacgccttg cggaaccagg
     2281 taacgccttg gtagcttctt ttttccttcg ccggcgcggg tgtcactgat caacaacggt
     2341 cggccgttag ccgccaattc aatgacaaag ggaatttctt tttcttccag accgaaggcc
     2401 ggtaaacggc gggccggatc aggatcgagc gccttgcgct ggtagtagtc gtagagggct
     2461 cgcaggatca tcccatcacc tgcgctttgc tcaggtcgat gatgccgttt tcaagctggg
     2521 cacggaaaaa gcgcggtttc gggtcatccg agtgttcaaa atccatgtca tacagcatcc
     2581 agccaaggtc gtggctttcg ttgagtggcg aggcttcaag atcaatctct gaaaccaaac
     2641 gaaagcgtga agcaaattcg cggcaaccca gatagggttg attgacgcat tgccctttct
     2701 cggcacgccg ctgaaacatg tcccggtatt tgccgggatt actcaacgca tcgttgccaa
     2761 ctggcaccaa gtcagcgtgc agtcgatagg cgacatcccg cagcagcagg ctggcccgtt
     2821 gctggcgctc ctcttcgata tacagcgcaa gattaccttt gccggcaccc atcgccgctt
     2881 gagcatttct gaccgatagc ttggacgaaa cctcattgcg ccgaacgctg atccagcgaa
     2941 tcggcttcag tacctcaatc cgcttcactt cccagcggat cgccggcttc cagagaatgg
     3001 cctcgaaaat cgagcgcgcc gccgacggcg ttatgacgtc gtaggacacc cgttcgacct
     3061 tcatttccgg tcgcgtgaag caggcgtagt cacctgaaac ctcaagacaa aaattatcca
     3121 agacagcctc cttgcaagca cttgccaaaa ctcatgagca gccccgcaac aaatgcggaa
     3181 cggagcagca caccacctat gaaaacaaaa agcactaacc ccgctaccgc ataaaaccga
     3241 tgcgacggtt gatctttcat caaccgcaac tcacgaataa gtccaagcaa attcataatc
     3301 gccgcccttg gccaagaaac ggttttgatt
//
LOCUS       DAORVN010000165         3309 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_24648_length_3309_cov_3.089121, whole genome shotgun sequence.
ACCESSION   DAORVN010000165 DAORVN010000000
VERSION     DAORVN010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3309
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24648_length_3309_cov_3.089121"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(79..681)
                     /gene="recR"
                     /locus_tag="PKV23_04370"
     CDS             complement(79..681)
                     /gene="recR"
                     /locus_tag="PKV23_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019172660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HQX84705.1"
                     /translation="MKRTAGIEIERLIQLLAKLPGLGPRSARRAALYLIKNREKLLSP
                     LALAMADAQDKVTVCSTCGNVDTIDPCTICCDPRRDRTILCVVEEVGDLWALERAGAW
                     TGLYHVLGGTLSAMEGVRPEDLAINHLIERAAASGMREVVLATNATVEGQTTAHYITE
                     RLKGLGLSTSRLAHGVPVGGELDYLDEGTLTAAMRARRPF"
     gene            complement(683..1006)
                     /locus_tag="PKV23_04375"
     CDS             complement(683..1006)
                     /locus_tag="PKV23_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HQX84706.1"
                     /translation="MKNLGDMMKQVQAMQSKMADMQAKLETATVTGQSGGGLVKVTLT
                     GKGAMSALNVDVSLLKEDEKEILEDLIVAAHADAKAKSEAMMAEEMKSVTGGLALPPG
                     MKLPF"
     gene            complement(990..2684)
                     /locus_tag="PKV23_04380"
     CDS             complement(990..2684)
                     /locus_tag="PKV23_04380"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF006585.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HQX84707.1"
                     /translation="MTETPDTMSAQGYRVLARKYRPQNFTDLIGQEAMVRTLTNAVAT
                     GRIAQAYMLTGVRGIGKTTTARLIARALNYPGSPSIEMPELTPQCEAILESRHMDVIE
                     MDAASNTGVDNMREIIDSVRYAPVALRYKIYIIDEVHMLSKSAFNALLKTLEEPPPHI
                     KFIFATTEIRKVPVTILSRCQRFDLKRLDAALLMQHYARIAALENVTIDEESLRMIAR
                     ASEGSVRDGLSLLDQAIAYGEGKVKATDVAGMMGLIDRSRIIDLFDAVMTGDPARAIH
                     EIEAQHEGGGDPETILNDLADFVHWVTRLKVVKESALTDPSRTEAEKLRGADYAARLG
                     MAHLTRAWQMLLKGIAEVSYAGQPLLAAEMVLIRLAHAADLPTGEELLKLAKQSPPAE
                     KSSPIVMAQPAKRELPVAESVGSLALKPEAAAPQAPTETRAFANFADIIALVVEKRDV
                     KLKGDLERFVRPIRVAPGQIELAMEQGASPGLPGELGRKLEAWTGRRWMVLVAKEGGD
                     KPLALQKKDAKESAFREAREEPAVQAILKRFPGSTVTDVRDPETLKPVEESDEESR"
     gene            complement(2705..2801)
                     /gene="ffs"
                     /locus_tag="PKV23_04385"
     ncRNA           complement(2705..2801)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PKV23_04385"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(2870..3160)
                     /locus_tag="PKV23_04390"
     CDS             complement(2870..3160)
                     /locus_tag="PKV23_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017965212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HQX84708.1"
                     /translation="MARYIVLANWTDQGVKNIKDSANRLDAAKALAKKMGCEMHEFYM
                     TVGAYDMVVMAEAPNDETFAKFTLNLAMGGNIRTTTLKAFPEDAYRKIIGGL"
BASE COUNT          568 a          971 c         1054 g          716 t
ORIGIN      
        1 tggacgcaaa ggagcaagcc acggtccaaa cgggccccgc gggacgttaa cttactggca
       61 aggtttacag actgttaatt aaaatggtcg ccttgcacgc atcgcggcgg tcagggtgcc
      121 ttcatccagg taatcgagct cgccgcccac gggcacgcca tgagccaggc gcgaggtgct
      181 caggcccagc cccttcaggc gctcggtgat gtagtgggcg gtagtctggc cctcaaccgt
      241 ggcattggtg gccagcacga cctcgcgcat gccggacgct gcggcgcgct cgatcaggtg
      301 gttaatggcc aggtcttcgg ggcgcacgcc ctccatggcg gaaagggtgc cgccgaggac
      361 atggtaaagg ccggtccagg cgccggcgcg ttcgagggcc cagaggtcgc ccacttcctc
      421 cacaacgcag agaatggtgc ggtcgcggcg cgggtcgcag caaatggtgc agggatcaat
      481 cgtatcgaca ttgccgcagg tgctgcacac cgtcaccttg tcctgggcat cggccatggc
      541 aagcgccagg ggagagagca gtttttcgcg gttcttgatt aggtaaaggg cggcgcggcg
      601 ggccgagcgg ggcccgagtc ccggcaattt tgccagcagc tggatgagcc gctcgatttc
      661 aatgcccgcc gtccgcttca tgtcagaagg gcagcttcat gccgggcgga agggccaggc
      721 cgccggtcac ggatttcatt tcctcggcca tcatcgcttc gctcttggcc ttggcatcgg
      781 catgggccgc cacgatgagg tcttcgagaa tttccttctc gtcttccttc agcagggaaa
      841 catccacgtt aagcgccgac atggcgccct tgccggtaag cgttaccttc accagcccgc
      901 cgccggactg gcctgtgacg gttgcggttt ccagctttgc ctgcatgtca gccattttgg
      961 attgcatggc ctggacctgc ttcatcatgt caccgagatt cttcatcact ctcttccact
     1021 ggtttcaagg tttctggatc gcgcacatcg gtcaccgtgc tgccgggaaa gcgcttgagg
     1081 atggcctgca cggcaggctc ttcgcgggcc tcgcggaaag ctgattcctt tgcgtctttt
     1141 ttctggagcg ccagcggctt gtcgccgccc tccttggcca caagcaccat ccagcggcgg
     1201 ccggtccagg cttcaagctt tcgtccgagt tcaccgggca agccgggcga agcgccctgc
     1261 tccatggcca gttcaatctg gccgggggcc acgcggatcg gcctgacaaa gcgctcaagg
     1321 tcgcctttca gtttcacgtc gcgcttttcg acaaccaggg caatgatatc ggcgaaattt
     1381 gcgaaggccc tggtttcggt gggcgcctgg ggggccgctg cttcgggctt cagcgccagc
     1441 gagcccacgg attcggccac cggcagctcg cgttttgcgg gctgggccat gacgatgggc
     1501 gatgattttt ccgccggcgg gctctgcttt gcaagcttca gaagctcctc acccgtgggc
     1561 aggtcagccg catgggcaag gcggatgagc accatttccg cggcaagcag gggctggccg
     1621 gcgtagctca cctctgcgat gcctttcagc agcatctgcc aggcgcgcgt cagatgcgcc
     1681 atgccaaggc gcgccgcgta atcggcgccg cgcagttttt ccgcctcggt gcgcgaagga
     1741 tcggtgaggg ctgactcctt caccactttc agccgggtga cccaatgaac gaaatccgca
     1801 agatcattga ggatggtttc cggatcgccg ccaccttcgt gctgggcttc aatctcgtga
     1861 atggcccttg ccggatcgcc ggtcatcacc gcgtcaaaca ggtcaatgat ccgcgagcgg
     1921 tcgatgaggc ccatcatgcc ggcaacgtcg gtggccttca ccttcccttc gccataggct
     1981 atggcctgat cgagcagcga caggccatcg cgcaccgaac cctcggaagc ccttgcaatc
     2041 atgcgcagcg attcctcgtc gatggtgaca ttctcgaggg ccgcgatgcg ggcgtagtgc
     2101 tgcatgagaa gggccgcgtc caggcgcttc aggtcaaagc gctggcagcg cgacaggatg
     2161 gtgaccggca ctttgcggat ttcggtggtg gcaaagatga acttgatatg cggcggcggc
     2221 tcttccaggg tcttgagcag ggcattgaag gcgctcttgg agagcatgtg gacttcgtcg
     2281 atgatgtaga tcttgtaacg cagcgccacg ggcgcatagc gcacgctatc gatgatttcg
     2341 cgcatgttgt cgacgccggt gttggaggcc gcgtccattt caatcacatc catgtggcgc
     2401 gattcgagaa tggcctcgca ctggggggtg agttccggca tttcgatgga agggctgcct
     2461 ggataattca gagcccgggc gatgagtcgg gcggtggtgg ttttgccgat gccgcgtacg
     2521 ccggtcagca tataggcctg agcgatccgc ccggtggcca cggcgttggt cagggtgcgc
     2581 accatggctt cctggccgat caggtcagtg aagttctgcg gacggtattt gcgggcgaga
     2641 acgcggtagc cttgagcgct catggtgtct ggggtttccg tcatgatggc tcagaatact
     2701 agaggtggga ggctggacgg gcaacccgtt ccgatctcgt tggggctgct tccttccgga
     2761 cctgacccgg ttggcgagcg gtacgtccgc cgccaacctc ccgaaggggt atatcggtgt
     2821 tttgggttga aaaggcaagg gtgggattca cccacctttg ccgttgctgt cacaagccgc
     2881 caatgatctt gcggtaggcg tcctcgggga aagccttgag ggttgtcgtg cggatattgc
     2941 ctcccattgc caggttcagg gtgaatttgg caaaggtttc gtcattgggg gcctcggcca
     3001 tgacaaccat gtcataggcc ccgacggtca tgtagaattc gtgcatttcg cagcccattt
     3061 tcttggctag cgctttggcc gcgtcgagcc gatttgcact gtccttgatg tttttgacac
     3121 cctgatcagt ccagttcgca agcacaatat atctggccat gtagtcctcc catttgcttt
     3181 tcgcacaagg gggcgattat ccgcctctcc ggcgaaacgg ggcaagaata acagattttt
     3241 gcaggtttga caatgcgccg ggcttggcct gattgacctc ttaggcccgc tcgcttatct
     3301 gagggccat
//
LOCUS       DAORVN010000166         3308 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_24660_length_3308_cov_2.635413, whole genome shotgun sequence.
ACCESSION   DAORVN010000166 DAORVN010000000
VERSION     DAORVN010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3308)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3308)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3308
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24660_length_3308_cov_2.635413"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..640
                     /locus_tag="PKV23_04395"
     CDS             2..640
                     /locus_tag="PKV23_04395"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011424991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HQX84709.1"
                     /translation="MLGPPGAGKGTQAKLLEVGRGMKQLSTGDMLRAAVAAGSEVGLQ
                     AKAIMDRGDLVSDEVILAVVGERMNQPDITAGVVFDGFPRTPAQAEALDGMLAARGQR
                     VDAVIEMKAEDEELVKRISGRFTCASCGKGYHDSFDKPKKDGICDVCGGTQFTRRADD
                     NEKTVRDRLTVYNKQTAPLVNYYAKRGNLHGVDGMADIKLVTASIGRILDGI"
     gene            840..1208
                     /gene="rpsM"
                     /locus_tag="PKV23_04400"
     CDS             840..1208
                     /gene="rpsM"
                     /locus_tag="PKV23_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008963796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HQX84710.1"
                     /translation="MARIAGVNIPTNKRAVIALRYIHGIGPVNAVEIMDKVKIPHSKR
                     VNELSDAEVLAIREVIDRDYMVEGDLRREVSINIKRLTDLGCYRGTRHRKSLPVRGQR
                     THTNARTRKGPAKAIAGKKK"
     gene            1220..1609
                     /gene="rpsK"
                     /locus_tag="PKV23_04405"
     CDS             1220..1609
                     /gene="rpsK"
                     /locus_tag="PKV23_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007603045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HQX84711.1"
                     /translation="MAKEAARPRRKERKNISSGVVHVNSSFNNTMITITDVQGNTIVW
                     ASAGKLGFKGSRKSTPYAAQMAGEQVARAAMEHGMRTVEVEVTGPGSGRESALRALQA
                     CGLQVTSIRDVTPIPHNGCRPPKRRRV"
     gene            1708..2730
                     /locus_tag="PKV23_04410"
     CDS             1708..2730
                     /locus_tag="PKV23_04410"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HQX84712.1"
                     /translation="MHVNQKNWQELIRPTKLDIQPGRERRKVATMVAEPLERGFGTTL
                     GNALRRILLSSLQGAAVSSVQIDGVLHEFSSIAGVREDVTDIVLNIKEIALAMHAEGP
                     KRVSLRKEGPGVVRAGDIQQTADIHVLNPEHVICTLDEGAEVRMEFTVNTGKGYVSSE
                     RNRPEDAPIGLIPVDSIYSPVRRVSYKIENTREGQILDYDKLIMTVETDGSMTPEDAV
                     AYAARILQDQLTVFINFAEPSKVVLEEARPELEFNAALLKKVDELELSVRSANCLKND
                     NIVYIGDLIQKSEAEMLRTPNFGRKSLNEIKEVLAQMGLHLGMEVPNWPPENIEDLAK
                     KFETHY"
     gene            2778..3206
                     /gene="rplQ"
                     /locus_tag="PKV23_04415"
     CDS             2778..3206
                     /gene="rplQ"
                     /locus_tag="PKV23_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008597535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HQX84713.1"
                     /translation="MRHGFRGRRFNRTVPHRLAMFANMSAALIKHEQIVTTLPKAKDL
                     RPVIEKLITLAKKGGLHAQRQAMSQVRDETQVKKLFDILAARFKDRQGGYARIMKAGF
                     RHGDNADMAVIEFVDRDTSAKGANDKVRVEAERAAEAEAA"
BASE COUNT          747 a          918 c         1010 g          633 t
ORIGIN      
        1 cctgctcgga cctccgggtg ccggaaaggg cacgcaagcc aagctgcttg aggtcggccg
       61 gggcatgaag caactttcca cgggcgacat gctgcgtgcg gcggttgcgg cgggaagtga
      121 agttggcctc caggccaagg cgatcatgga ccgcggcgat ctggtatctg acgaagtcat
      181 cctagcggtg gtgggcgagc ggatgaacca gccggacatc acggccgggg tggtttttga
      241 cgggtttccc cgcacgccgg cccaggcgga ggccctggac ggcatgctgg cggcgcgggg
      301 ccagagggtt gatgcggtga tcgaaatgaa agccgaggac gaggaactgg tgaaacgcat
      361 ttccggccgt ttcacctgtg cttcgtgcgg caagggctat catgacagtt tcgacaagcc
      421 gaaaaaggac ggaatttgcg acgtgtgcgg gggaacccaa ttcacccggc gcgccgacga
      481 caacgaaaag acggtaagag accgcctgac ggtctacaac aagcagacag cccccctggt
      541 aaactactat gccaagcggg gcaatttgca cggggtggac gggatggcgg acatcaagct
      601 ggtgacggcc tcgatcggcc gcattcttga tgggatttag gccagttttg ttggtcaatc
      661 gggttgacgc gtaagcgatt ccagcctatt gaaccccaca atcttggtct gcatatgagc
      721 tggcttgggg agggaaacct tccagagcgc ggctttgcgc tattgaagcg tcaggtgtga
      781 ctataacaga aatgcgccct tggacagggt aagtccttgg aaagcttagg agaaacagag
      841 tggcccgtat tgcaggcgta aatattccaa ccaacaagcg cgccgtcatt gcgctgcgct
      901 atatccatgg aatcggtccg gtcaacgcgg tcgagatcat ggacaaggtc aagatccccc
      961 attccaagcg ggttaacgag ttgagcgatg ccgaagtgct ggcaatccgc gaagtgattg
     1021 accgcgatta catggtagag ggtgaccttc gccgtgaagt ttccattaat atcaagcgct
     1081 taactgatct gggctgctat cgcggcaccc gtcaccgcaa gagcctgccg gttcgcggcc
     1141 agcgcaccca caccaatgca cgcacacgca agggccccgc taaggccatc gccggcaaga
     1201 agaagtaagg gctcagatca tggctaaaga agcagcacgt ccgcggcgca aagagcgcaa
     1261 gaacatctcg tccggcgtgg ttcacgtcaa ctcatcgttc aacaacacaa tgatcaccat
     1321 caccgacgtc cagggcaaca cgattgtctg ggcctcggcc ggtaaactcg ggttcaaagg
     1381 ttcgcggaag tcgacacctt acgccgccca gatggcaggc gagcaggttg cccgcgccgc
     1441 catggagcat ggcatgcgga cggttgaagt tgaagtcacc ggtccgggtt cgggccgtga
     1501 gtcggcttta cgcgccttgc aggcctgcgg cctgcaggtg acgtcgatcc gcgatgtaac
     1561 gccaattccg cacaatggct gccgcccacc aaagcggcgc cgcgtctaac aggttagtca
     1621 tgggttttcc tttgggaagc ccagtaaaag aggctgagaa ccggagggca ttttcctgcc
     1681 cacccgggaa atccacggga tagcaaaatg catgtaaatc aaaaaaactg gcaggaactc
     1741 atcaggccaa caaagctcga catccagccg ggccgtgaac gccgcaaggt tgccactatg
     1801 gtggccgagc ctttggagcg tggtttcggc acgacgctcg gcaatgcgct gcgccgcatt
     1861 cttctctcgt cacttcaggg tgccgctgtt tcttcagttc agatcgatgg cgtgctgcac
     1921 gagttctcct cgattgccgg ggtgcgcgaa gacgtgaccg acatcgtttt gaacatcaag
     1981 gaaattgccc tggccatgca tgcggaaggc cccaagcggg tttcgctgcg caaggaaggc
     2041 ccgggcgtgg tccgcgccgg cgacattcag cagaccgccg acattcatgt gctcaacccc
     2101 gagcatgtga tctgcaccct ggatgaaggt gctgaagtgc gcatggaatt caccgtgaac
     2161 acgggcaagg gctatgtttc ctccgagcgc aaccgcccgg aagatgcgcc catcggcctg
     2221 atcccggttg actcgattta cagcccggtg cgccgcgtat cctacaagat cgagaacacc
     2281 cgcgagggcc agattctcga ctacgacaag ctgatcatga cggttgaaac cgatggttcg
     2341 atgacccctg aagacgctgt tgcctatgcg gcgcgcatcc tgcaggacca gctcacggtg
     2401 ttcatcaact tcgccgagcc ttccaaggtt gtgcttgaag aagcccgtcc cgaactggag
     2461 ttcaatgccg cccttctcaa gaaggttgac gagcttgaac tttcggtgcg ctcggccaac
     2521 tgcctgaaga acgacaacat tgtttacatc ggcgacctga tccagaagtc ggaagcggaa
     2581 atgctccgta ccccgaactt cggccgcaag tcgctgaacg agatcaagga agttctggcg
     2641 cagatgggcc tgcatctcgg catggaagtg ccgaactggc cgccggaaaa catcgaagat
     2701 ctcgccaaga agtttgaaac ccactattga gaaattaagc gcctggccga ggccgggcgg
     2761 gtttaaggag aacggttatg cgtcatggtt ttagaggtcg ccggttcaac cgcacggttc
     2821 cgcatcgtct ggcgatgttt gccaacatgt cggcggcatt gatcaagcat gagcagatcg
     2881 tcactaccct gccaaaggcc aaggatttgc gcccggtgat cgagaagctg atcacgctcg
     2941 ccaagaaggg cgggctgcat gcacagcgcc aggccatgag ccaggtgcgc gatgaaacgc
     3001 aggtaaagaa gctttttgat attctcgccg cgcgcttcaa ggaccgccag ggcggttatg
     3061 cccgcatcat gaaggcgggt ttccgccatg gtgacaatgc cgatatggcc gtgatcgaat
     3121 tcgtggatcg cgacacgtcc gccaagggcg cgaacgacaa ggttcgcgtt gaagccgaac
     3181 gcgctgccga agcggaagcc gcgtaagcta cgctttgctt gtgattagaa ttcaaagccc
     3241 ggctgtaacg ccgggctttc attattagac tatgcggcaa gcgattggcg gcgatttatt
     3301 gatttaag
//
LOCUS       DAORVN010000167         3301 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_24746_length_3301_cov_3.805915, whole genome shotgun sequence.
ACCESSION   DAORVN010000167 DAORVN010000000
VERSION     DAORVN010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3301
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24746_length_3301_cov_3.805915"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..503)
                     /locus_tag="PKV23_04420"
     CDS             complement(<1..503)
                     /locus_tag="PKV23_04420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX84714.1"
                     /translation="MKNFLNSFAGFLPLGILWLSPLAAGLVAVLYGALDEAAWLALFG
                     HPQLWPGLALSMGTGTLSLIISALLAFWIVAGLYGGKLWPGTSGLMGGFLSLPHLAFA
                     IGLGFLITPSGLLARVIGSVAAWAAPPQWVTTQDPYGFSLVTVLVLKEVPFLIWLISA
                     HLARPEVA"
     gene            complement(500..1699)
                     /locus_tag="PKV23_04425"
     CDS             complement(500..1699)
                     /locus_tag="PKV23_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003531965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HQX84715.1"
                     /translation="MKFTRRDLVRGAGAAALLPLFPRAGIAGDWAAVEAEARGQTVYF
                     NAWAGSEAINAYIDWAAEILKARHGVTLEHVKIADAAEVVRRVRDEVKAGKAEGSADL
                     VWINGENFRAMKAEKLLYGPFATDLPNFRYVDTEGKPTTLVDFAEPTDGLESPWGMAQ
                     LTFFGDGAKISTPPQSMDELVAFAKAHPGRVTYPAPPDFHGTTFVKQALIESIVDQGL
                     LPLPAKDGEFLRAAAPLFDLLDRLHPHLWRAGKQFPQSQAQLKQMLADGELLMALTFN
                     PNEPANLIAAGALPDTTVAWQHRAGTIGNTHFVAIPVNSKATAGARVVASFLLSPEAQ
                     GRKADLKTWGDPTVLAITKLSAEEAKFFGTSIVPGVVTEPAPALAEPHGSWVPLLEKA
                     WLESYGT"
     gene            complement(1794..>3301)
                     /locus_tag="PKV23_04430"
     CDS             complement(1794..>3301)
                     /locus_tag="PKV23_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008194522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HQX84716.1"
                     /translation="ETASAGIDSVYAGEWEYMVGGGAATFDCNGDGFADMLLAGGTAP
                     AKFYRNASSRGGALKFEAEASGLELEAVTGVYPLDVDSDGNTDLVLLRVGENVVMRGL
                     GNCRFERANEAWGFDGGNAWSTAFAATWEKGAAWPTLAIGNYINRLEETSPWGSCTDN
                     WLHRPDAKPNKFAAPLALKPSYCALSMLFTDWNKSGTPSLRISNDREYYEGGQEQLWH
                     VDAGQAPALYTEKEGWKPLRIWGMGIAGYDLDSDGYQEYFLTSMADNKLQTLASAPMD
                     GKPPEATFKDVAYARGVTAHRPYTGGDLRPSTAWHTQFEDVNNDGLVDLFIAKGNVAK
                     MPDFAAKDPNNLLLQGADGKFTESGEAAGVASLAISRGGALADFNMDGLLDLVVVNRW
                     EKAQVWRNTSTNAGHWIEVKLQQPGTNRDAIGAWLEVKQGEKILRREIFVGGGCASGQ
                     NGWWHFGLGDMAQAEVRVIWPDGTSDGWQSVNSDNFYILERSKAAQVWAAK"
BASE COUNT          626 a         1069 c          971 g          635 t
ORIGIN      
        1 gcgacctcgg gccgcgccag gtgtgcgctg atgagccaga tcaggaatgg cacttccttg
       61 agaaccagaa ccgtcaccag agaaaatcca taaggatcct gggtcgtcac ccattgcggc
      121 ggcgcggccc aggcggcaac cgagccgatg acccgcgcca ggaggccgct gggcgtgatc
      181 aggaagccaa gcccgatggc aaaggccagg tgcggcaggg acaggaagcc gcccatgagc
      241 cccgaggttc ccggccagag ttttccgccg taaagtcccg ccacgatcca gaaggcgagc
      301 aacgccgaaa tgatgaggct cagtgttccc gtgcccatgg aaagcgccag ccccggccag
      361 agctgcggat ggccaaacag ggcaagccag gcagcctcat caagggcgcc atacaacacc
      421 gcaacaaggc ctgccgccag cgggctcagc cataagatgc caagcggcag aaaaccggca
      481 aagctgttca gaaagttttt catgtgccgt aggattcaag ccaggccttt tcaagcagcg
      541 gaacccagct cccgtgcggc tcagctagcg ccggcgccgg ttcagtcaca actccgggga
      601 caatgctggt tccaaaaaat ttggcctctt cggcggaaag tttggtgatt gcaagcaccg
      661 tgggatcgcc ccaggtcttg agatcggcct tgcggccctg cgcctccggc gaaagcagga
      721 agctggccac aacccgggct cccgccgttg cctttgaatt aaccggaatg gccacgaaat
      781 gcgtgttgcc aatggtgccg gcccggtgct gccacgccac tgtggtgtcc ggcaacgccc
      841 ccgcggcgat gagattggca ggctcattgg gattgaaggt gagcgccatc agcaattcgc
      901 cgtccgccag catctgcttg agctgtgcct ggctctgcgg aaactgcttg cccgctcgcc
      961 agagatgcgg atgcagtctg tcgagcaggt caaaaagggg tgcggcggcg cggagaaact
     1021 caccgtcctt cgcgggcagc ggcagaagtc cctgatccac gattgactca atcagcgcct
     1081 gcttcacgaa agtcgtgccg tgaaagtcag gtggggcagg ataggtcacg cggcccggat
     1141 gggctttggc aaacgccacg agttcgtcca tgctttgggg cggcgtggag attttggcac
     1201 catcaccgaa aaacgtgagc tgcgccatgc cccaggggct ttccaggcca tcggtcggct
     1261 cggcaaaatc cacaagcgtc gtgggcttgc cctcggtgtc cacataacgg aagtttggca
     1321 ggtcagtggc gaatggcccg tacaaaagct tctccgcttt catggcgcgg aaattttcac
     1381 cattgatcca gaccagatcc gccgaacctt cggccttgcc ggctttcact tcgtcacgca
     1441 cccgccggac cacttccgcc gcgtcggcaa tcttcacatg ctcaagggtg acgccatgac
     1501 gggccttcag aatttccgcc gcccagtcga tataggcatt gatggcttcc gaacccgccc
     1561 aggcgttgaa gtaaaccgtc tggccccggg cctccgcttc aactgcggcc caatctcccg
     1621 caatgcccgc tctcgggaaa agcggcagca aggcagcagc gcctgctccc ctcaccaaat
     1681 cacgcctggt aaatttcatg acggctcctg tatcgccgcc cactcatagg gagttccggg
     1741 acgaaaggaa atatatccct ccgtgctgta atcagaaatc tgtgatcagc ggtttacttt
     1801 gccgcccaga cttgtgccgc tttgctccgc tcgagaatgt aaaaattgtc gctgttaacg
     1861 ctttgccagc catcggaggt gccgtctggc cagatcacgc gcacctctgc ctgagccatg
     1921 tcgcccagcc cgaaatgcca ccagccattc tgcccgctgg cgcatccgcc gcccacgaag
     1981 atttcccggc gcaagatctt ctcgccttgt ttaacttcaa gccaggcgcc gatcgcgtcg
     2041 cggtttgtgc cgggttgctg cagcttcacc tcgatccagt ggccggcatt ggtgctggtg
     2101 ttgcgccaca cctgggcttt ttcccagcgg ttgaccacaa ccagatccag caggccgtcc
     2161 atgttgaaat ccgccagtgc gccgccgcgc gaaatggcga ggctcgccac gcctgccgcc
     2221 tcgccggatt cggtgaactt gccgtcggcg ccctgcagca gcaggttgtt ggggtccttc
     2281 gccgcgaagt ccggcatttt cgccacattg cccttggcaa tgaacaggtc aaccaggccg
     2341 tcattgttca cgtcctcaaa ctgcgtatgc caggccgtgc tcgggcgcag gtccccgccc
     2401 gtgtaggggc ggtgtgccgt gactcccctg gcataggcca catccttgaa ggtggcctcc
     2461 ggcggtttgc cgtccatggg tgcgctggcc agggtctgca gcttgttgtc ggccatgctg
     2521 gtcaggaaat attcctggta gccatcggaa tcgaggtcgt agccggcaat ccccatgccc
     2581 cagatgcgca agggtttcca cccttccttc tcggtgtaga gcgccggcgc ttgccccgca
     2641 tcaacatgcc agagctgttc ctggccgccc tcgtagtatt cgcggtcgtt ggatatgcgt
     2701 aaggaaggcg tgcctgactt gttccagtcg gtgaacagca tcgacagcgc gcaatagctg
     2761 ggcttgaggg ccagcggtgc cgcgaacttg ttcggcttgg cgtcaggccg gtgcagccag
     2821 ttgtccgtgc aggaccccca gggcgaagtc tcctccaggc ggttgatgta attgccgatg
     2881 gccagtgtcg gccacgcagc acctttctcc caggtggcag caaaggccgt tgaccacgcg
     2941 ttcccgccat caaaacccca ggcttcattg gcccgctcaa aacggcagtt gccaagtccc
     3001 cgcatcacga cattttcacc gacgcgcagc agcacgagat cggtgttgcc gtcactgtcg
     3061 acatccagcg gataaacccc ggtgactgct tccagttcga gcccgctggc ttccgcctcg
     3121 aatttcaggg cgccgcccct gctgctggca ttgcggtaga atttggctgg cgcggtgccg
     3181 cccgccagca gcatgtcggc aaagccgtcg ccattgcagt cgaaggtggc agccccgccg
     3241 cccaccatgt attcccattc gcccgcatat acgctgtcaa tcccggcact ggccgtttcc
     3301 t
//
LOCUS       DAORVN010000168         3280 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_25015_length_3280_cov_3.351938, whole genome shotgun sequence.
ACCESSION   DAORVN010000168 DAORVN010000000
VERSION     DAORVN010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3280
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25015_length_3280_cov_3.351938"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            64..774
                     /locus_tag="PKV23_04435"
     CDS             64..774
                     /locus_tag="PKV23_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010914114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HQX84717.1"
                     /translation="MVADNSRLTTVGLDADDTLWQSEQFFKLTEARFVELLTGYAEAG
                     DLSARLLQAEMKNLQYYGFGTKSFVLSMIETAIDVTQGKVPVRVIQEILSAGREMLAH
                     PIQPLPHVRETLEKLGNSFRLVLISKGDLFDQERKLAQSGLGEFFHGVEIVSEKTPQV
                     YRRIFDRHGDGPDRAVMVGNSMKSDIVPAIAAGCWGVFIPHELTWTLEHAEAPIHHPR
                     FRQLKSLNELHEFITKIS"
     gene            complement(795..869)
                     /locus_tag="PKV23_04440"
     tRNA            complement(795..869)
                     /locus_tag="PKV23_04440"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(835..837),aa:Gly,seq:gcc)
     gene            983..1486
                     /locus_tag="PKV23_04445"
     CDS             983..1486
                     /locus_tag="PKV23_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018699065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HQX84718.1"
                     /translation="MPASRDELFQRFADLGIKTTTKNHAPVFTVEEARLVHPDIPGGH
                     CKNLFCKDEKGVLWLIVCLEDSRVDLKAAPAKIGSRRLSFGKPELLMEVLGLEPGSVT
                     PFGLINDKNVRTNVILDARMMEEEILNYHPLKNDATTTIRSGDLLAFIRSCGHEPRVV
                     AVADPAL"
     gene            1546..2457
                     /gene="trxA"
                     /locus_tag="PKV23_04450"
                     /pseudo
     CDS             1546..2457
                     /gene="trxA"
                     /locus_tag="PKV23_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018388168.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
     gene            2464..3132
                     /locus_tag="PKV23_04455"
     CDS             2464..3132
                     /locus_tag="PKV23_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009338528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HQX84719.1"
                     /translation="MKFLKDYNRPADLPQKLPVFPLPGALLLPRADLPLNIFEPRYLE
                     MISDALSGERMIGIIQPRDDSDTAERPDLMKVGCAGRITSYAETPDGRMLVTLTGVSR
                     FSVKSELAVDTPYRQVVANFKPFAIDLVMDLGAAEVNRPALLTAFKDYLTANNMSADW
                     SEINAASTEVLVNTLSLLAPYPASEKQALLEAPDLKTRADVLVALTEMALSKSFSGKR
                     HKLQ"
     gene            3141..>3280
                     /locus_tag="PKV23_04460"
     CDS             3141..>3280
                     /locus_tag="PKV23_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019965359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HQX84720.1"
                     /translation="MHEVREVDPKLLEILVCPVTKTRLDYDAVRQELISKAAGLAYPI
                     RDG"
BASE COUNT          749 a          970 c          888 g          673 t
ORIGIN      
        1 aaagcatcga cgcccccaaa ccgtgcccga caggaacagc aaaactgtct agagattatc
       61 cttatggtag cagacaattc tcggctcaca acggttggac tggatgccga cgataccctt
      121 tggcagagtg agcagttttt caagctcacc gaagcccgct ttgttgagct tctcacagga
      181 tacgccgaag ccggcgatct gagcgcacgc ctgctgcagg cagaaatgaa aaacctgcaa
      241 tactatggct ttggcaccaa gagctttgtc ctctcgatga tcgaaactgc catcgacgtc
      301 actcagggca aggtaccggt ccgggtcatc caggagatcc tcagcgctgg ccgtgaaatg
      361 ctggcccatc ccattcaacc cctgccgcac gtacgcgaaa ctttggagaa gctgggcaat
      421 tcctttcggc ttgtcctcat tagcaagggt gatctcttcg accaggaacg caaacttgcc
      481 caatcaggct tgggagaatt ctttcacggc gttgaaattg tcagcgaaaa aacaccccag
      541 gtctaccggc gcatttttga ccggcacggc gatggtcccg accgcgccgt catggtggga
      601 aattccatga aatcagacat tgtaccggcc attgccgccg gctgctgggg cgtcttcatt
      661 cctcatgaat tgacctggac tctggaacat gccgaggcac cgatccacca cccgcgcttc
      721 aggcagctga aaagtcttaa tgaactccat gaattcatta caaaaatatc atgatgcggg
      781 gatgtcggga atattggagc gggcgaaggg attcgaaccc tcgaccccga ccttggcaag
      841 gtcgtgctct acccctgagc tacgcccgct gagcgtatga cgaagccgga aaaaaccggc
      901 gaggtggcgc gtcttattgc ccaagcccgg cgtaaatgca atagcaagtt caacgagccc
      961 ctaacctgga acgattgatg ccatgccagc cagccgcgat gagcttttcc aacgctttgc
     1021 cgaccttggc atcaaaacca cgacgaaaaa ccatgccccc gtattcaccg tggaggaggc
     1081 taggctcgtc caccccgata tccccggcgg ccactgcaag aacctcttct gcaaggatga
     1141 aaagggcgtc ctttggctga ttgtctgcct ggaggactcg cgcgtcgatc tgaaggcagc
     1201 cccggcaaaa atcggctcac ggcggctttc ttttggcaaa cccgaactgc tgatggaagt
     1261 tctgggcctt gaaccaggct cggtcacgcc ttttggcctc atcaatgata agaacgttcg
     1321 gactaacgtg attctcgacg cccggatgat ggaggaggaa atcctgaact atcatccttt
     1381 gaaaaatgac gccacaacga ccattcgttc cggcgatctg ctggccttca tcaggtcatg
     1441 cggccacgaa ccacgggttg tcgcagtggc cgatcccgca ttgtaatttg cccccagaag
     1501 cgccattttt acgatgaaac accaaacagg cacaacatga acgacatgat cataggacaa
     1561 accagcgctt ccgcggttcc cgccgctgaa cttataaagg ataccgatac caagagcttc
     1621 atggccgatg tcatcgaggt ctcaaagaag gtaccggttc tcgtggattt ttgggccccc
     1681 tggtgcggcc cctgcaagca gctgacgccg cttctcgaga aggtcgtgaa gcaggccaac
     1741 ggaaaagtgc gcctcgtcaa aatcaatgtg gacgaaaacc agcagattgc cgcccaaatg
     1801 cgggtgcaat cgattcccgc cgtatttgcc tttgtaaacg gccagcccat tgacggcttc
     1861 atgggcgccc tgcccgaggg ccagatcagg caattcgtcg acaagctttc gagcaaaggc
     1921 tcgctggccg aggagattgc tgccgccatg aaggtggccg aagaggccat gcagcgcaaa
     1981 gcctttgacg aagcccagaa cattctggga cagattctcg gcgtcgatcc cacccatgtt
     2041 gcagccctcg ccaattttgc aaaatgccag atcgagctcg gcgacctcga aagtgccgct
     2101 gcgaccctgg cccaggttcc agctgacaag gcggcaaacc ccgatgttgt cagcgctgcc
     2161 gctgcgcttg atctggcgct tcatcccgtc gacaccagtc tcctcgatca attgcaaaaa
     2221 gctgtggccg ccacccccga tgactttgca aagcgcctgg agctggccgt tgccttgaat
     2281 ggcgctggct agaggcccga ggccaccgac cagttgctct atgtcatccg caagaagcgc
     2341 gattggaatg aagacgcggc ccgcaagcag ctcgttcagt tctttgacgc ttgggggcca
     2401 agggatgagg ccaccctgca aggccgccgc aaactgtctg caactctatt ttcgtgagga
     2461 tgaatgaaat ttcttaagga ctataaccgg ccagccgacc tgccccagaa gctgccggtg
     2521 tttccgcttc ccggcgcctt gctgttgccg cgggctgatc tgccgctgaa tatctttgaa
     2581 ccccgctatc tcgagatgat ttccgatgcg ctgtcgggtg agcgcatgat cgggattatt
     2641 cagccccggg atgatagtga cacggccgaa cggccggacc tgatgaaagt gggttgcgcc
     2701 gggagaatca cctcttatgc ggaaaccccc gatggcagga tgctggtgac gctgaccggc
     2761 gtcagccgct tttctgtcaa gagcgaattg gccgtcgaca cgccctaccg ccaggtcgtg
     2821 gccaacttca aacctttcgc cattgacctt gtgatggatt tgggtgcggc tgaggtcaac
     2881 cggcccgccc tgctcaccgc cttcaaggat tacctcacgg ccaacaacat gagcgccgac
     2941 tggagtgaaa tcaatgcggc ctcgaccgag gttctggtca atacgctttc gcttctggcg
     3001 ccctatcccg ccagcgaaaa gcaggcgctt ctcgaagcgc ctgacttgaa aacccgtgcc
     3061 gatgtgttgg tagcgctgac tgaaatggcg ctgtcgaagt ccttctccgg caaacgccac
     3121 aaattgcagt aggaaaagcc atgcacgaag tccgcgaggt ggatccaaaa ttgcttgaga
     3181 tccttgtttg ccccgtgacc aagacccgtc tcgactatga cgcggtaagg caggagctca
     3241 tttcgaaagc ggcggggctt gcctatccaa tccgcgacgg
//
LOCUS       DAORVN010000169         3270 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_25157_length_3270_cov_3.348056, whole genome shotgun sequence.
ACCESSION   DAORVN010000169 DAORVN010000000
VERSION     DAORVN010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3270
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25157_length_3270_cov_3.348056"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1093
                     /locus_tag="PKV23_04465"
     CDS             <1..1093
                     /locus_tag="PKV23_04465"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011976260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HQX84721.1"
                     /translation="PWQPKTALVLCDLLDHHDHSPIPHAPRNILKRQLERLSAMKMKA
                     YFASELEFYVFDQSFKEAHAGGYRTLTTPSHLNEDYNILQTSKDENLMRAIRNGLHGA
                     GITVENSKGEAWAGQEEINVRYDAALPMADTHVFLKHACKEIALGLGQSLTFMAKPNY
                     AWAGSSSHIHQSLWSADGKQPLFFDKSGDHGMSDLMKHYVAGLLAHADEITYFLAPYI
                     NSYKRYIAGTFAPTRAVWSFDNRTAGYRLCGADSKAIRIECRVGGSDLNPYLAFAALL
                     AAGLDGIENKMALEPVFAGDAYDKSKKLREIPKTLREATALLDSSKFLRKALGDDVVD
                     HYVHTAKWEQFEYDRRVTDWEVRRGFEQY"
     gene            1102..2493
                     /locus_tag="PKV23_04470"
     CDS             1102..2493
                     /locus_tag="PKV23_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013893089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HQX84722.1"
                     /translation="MVETIKCFSPVDGKLYASRPVASKKEIAAAFSAAHAAQEKWKRV
                     SLKGRAAFCSAAVDAMLAMRDEIVPELAWQMGRPIRYGAGELRGFEERARHMIGIAEG
                     ALANHVPAAKEGFTRFISREPWGVVFTIAPWNYPYLTAVNSVIPALMAGNAVVLKHAS
                     HTLLVAERFQKAFDKAGLPKGVFQHLVMNHAQATDVISGGLVNMVCFTGSVAGGRAME
                     QAAAGRFINVGLELGGKDPAYVRHDANLAHAVENLVDGAFFNSGQSCCGIERIYVHKS
                     LYKDFVSGFADLTKKYVLGSPLDEATTLGPMIKPEAADFVRKQIKSALRGGAVAHVDA
                     KSFAMDKAGTAYMAPQVLTGVNHQMSVMTEESFGPVVGIMKVSDDAEAISLMNDSDYG
                     LTAALWTQDADAARMIGDELATGTVFMNRCDYLDPALAWTGVKNTGRGITLSALGYEA
                     MTRPKSYHLRTKL"
     gene            2490..>3270
                     /locus_tag="PKV23_04475"
     CDS             2490..>3270
                     /locus_tag="PKV23_04475"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003494812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HQX84723.1"
                     /translation="MTIPNRNFSYPTALKYGVGRIKELAELCKANGIMRPLFVTDPGL
                     AGMPMVHAIIADVKNAGLAIEIFSDVRPNPIEANILEGVKAYKAGRHDGVIAFGGGSG
                     LDIGKMIALMHGQVISVFELEDIGDWWTRADASRISPIIAVPTTAGTGSEVGRAGVVT
                     HPITHEKKIIFHPAIMPKVAILDPQLTVGLPPKLTAATGMDALAHCLEAYCAPFYHPL
                     SAGVGLEGMRLVKENLAKAVKKGSDLDARGNMLMASTMGATA"
BASE COUNT          680 a          941 c          990 g          659 t
ORIGIN      
        1 tccatggcag ccaaagacgg cgctggtgct ctgtgacctg ctcgaccacc atgatcattc
       61 gcccattccg catgcgccgc gcaacatcct gaagcgccag ctggagcggc tttctgccat
      121 gaagatgaag gcctacttcg cctcagaact tgaattctat gtcttcgacc agagcttcaa
      181 ggaagcccat gcgggcggct accgcacgct cacaacaccg tcgcacctca acgaagatta
      241 taacatcctg caaaccagca aagacgagaa cctgatgcgc gccattcgca acggtctcca
      301 tggtgccgga atcacggttg agaactccaa gggggaagcc tgggcgggcc aggaggaaat
      361 caacgtgcgc tatgacgcgg cattgcccat ggccgacacc catgtgttcc tgaaacacgc
      421 ttgcaaggaa atcgccctcg ggcttggcca gtcgctcacc ttcatggcca agcctaacta
      481 cgcatgggcg ggaagttcgt cccacatcca ccaatccctg tggagtgctg atggcaagca
      541 gccgctgttc tttgacaaga gcggcgacca tggcatgtcg gacctgatga agcattatgt
      601 ggcgggcctg ctcgcccatg ccgacgaaat cacctatttc cttgcgccct atatcaactc
      661 ctacaagcgc tacatcgcag gaacctttgc gcctacccgc gccgtgtgga gttttgacaa
      721 ccgcactgcg ggttatcgtc tctgcggtgc tgacagcaaa gctatccgca ttgaatgccg
      781 cgtcggcggc tctgacctca acccctatct agcttttgcg gcactgcttg cggcaggtct
      841 ggatggtatt gagaacaaga tggcattgga acctgttttt gcgggggatg cctatgacaa
      901 gtccaaaaag ctgcgcgaaa tccccaagac gctccgtgaa gcgacggcgt tgctcgacag
      961 ttcaaaattt ctccgcaaag cgctgggcga tgatgttgtc gatcactacg ttcacacggc
     1021 gaaatgggag cagtttgaat atgaccggcg cgtcaccgac tgggaagtgc gccgcggctt
     1081 tgagcagtat taggggttat catggttgaa acaatcaagt gtttctcgcc ggtagacggc
     1141 aagctctatg ccagccgccc ggtggcttcc aaaaaagaaa ttgcggccgc gttttccgcg
     1201 gcccacgcag cgcaggaaaa atggaagcgc gtttccttga aggggcgcgc ggctttctgc
     1261 tccgccgctg tcgatgccat gctggccatg cgggatgaaa ttgttccgga gcttgcctgg
     1321 cagatgggcc ggcccatccg ctatggcgcc ggcgagttgc gcggctttga agagcgtgcg
     1381 cggcacatga tcggcatcgc ggaaggagcc ttggccaatc atgttcccgc cgcaaaggaa
     1441 ggcttcacgc ggtttatcag ccgggagccc tggggcgtgg ttttcaccat cgcgccctgg
     1501 aattacccgt atctcacggc ggtgaattcg gtcattccag ccctgatggc gggcaatgcg
     1561 gtggtgctga aacacgcctc ccacaccctg ctggtggctg aacgcttcca gaaggccttc
     1621 gacaaggctg ggctgccgaa aggggttttc cagcatctgg tgatgaacca cgcacaagcc
     1681 accgacgtta tttccggcgg gctcgtcaac atggtgtgtt tcaccggctc ggtggccggc
     1741 ggcagggcca tggagcaggc ggcggccggt cgcttcatca atgtgggcct ggaacttggc
     1801 ggcaaggatc cggcctatgt gcggcacgac gcgaacctgg cccatgcagt ggaaaacctg
     1861 gtggacgggg cgtttttcaa ttcaggccaa agctgctgcg gcattgagcg catttatgtg
     1921 cacaagagcc tttacaagga tttcgtttcc ggatttgcag atctgacaaa aaaatatgtg
     1981 ctgggttcgc cgctcgatga agcgacaacg cttgggccga tgatcaagcc ggaggccgcc
     2041 gatttcgtcc gcaagcaaat taagtcggcc ttgcgcggcg gggctgtggc gcatgtcgat
     2101 gcaaaatcat ttgccatgga caaggccgga acggcttaca tggcgcctca ggtgctgacc
     2161 ggggtgaatc accagatgag cgtgatgacc gaggaaagct tcgggcccgt cgtgggcatc
     2221 atgaaggttt ccgacgacgc ggaagcaatc tcgctgatga atgattcgga ttacggcctc
     2281 actgccgccc tgtggacaca ggacgccgat gcggcccgga tgattggcga cgagcttgca
     2341 accggcaccg tgttcatgaa ccgctgcgat tatctcgatc cggcgctggc ctggaccggg
     2401 gtcaagaata cgggccgcgg catcaccctt tccgcactcg gctacgaagc catgaccaga
     2461 ccgaaaagct atcatttgag gacaaagcta tgacaattcc aaaccgcaat ttcagttatc
     2521 ccacggcgct caagtatggc gtggggcgca tcaaggaact ggccgaactg tgcaaggcca
     2581 acggcatcat gcggccgctc ttcgtcaccg acccgggatt ggcgggcatg ccgatggtgc
     2641 atgccatcat tgccgatgtg aaaaatgccg gactggcgat tgaaatattt tccgatgtgc
     2701 ggcccaatcc gatcgaggcc aatatcctgg agggcgtcaa ggcctacaag gcggggcggc
     2761 atgacggcgt gatcgccttt ggcggcggct cgggccttga tatcggcaag atgatcgcgc
     2821 tcatgcacgg ccaggtcatt tcggtgttcg agcttgagga catcggtgac tggtggacta
     2881 gggccgatgc atccaggatt tcgccgatta tcgcggtgcc gacaacggcg ggcaccggct
     2941 ctgaagtggg ccgcgccgga gtggtcaccc atcccatcac ccatgagaag aaaatcattt
     3001 tccatccggc gatcatgccg aaggtggcga tcctcgatcc gcagctcacg gttggcctgc
     3061 ctcccaagct gacggcggct acgggcatgg atgcgctggc ccattgcctg gaagcctatt
     3121 gcgcgccgtt ttaccatccg ctgtcggccg gtgtcgggct tgagggcatg cgcctggtca
     3181 aggaaaacct ggcgaaggcg gtgaagaagg gttcggacct tgatgcgcgc ggcaacatgc
     3241 tgatggcctc caccatgggc gccaccgcct
//
LOCUS       DAORVN010000170         3267 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_25204_length_3267_cov_2.849004, whole genome shotgun sequence.
ACCESSION   DAORVN010000170 DAORVN010000000
VERSION     DAORVN010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3267
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25204_length_3267_cov_2.849004"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="PKV23_04480"
     CDS             <1..313
                     /locus_tag="PKV23_04480"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HQX84724.1"
                     /translation="NVPKEGSQLWFDLLVIPNKSKNKDGAYQLLNYMLKPEVAAANTN
                     FLQYANPILTSTPYIEPKLLGDPGLYPPKSVLKRLAVQPPMAANVEAELKRIWSKLSK
                     E"
     gene            517..>804
                     /locus_tag="PKV23_04485"
     CDS             517..>804
                     /locus_tag="PKV23_04485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyltransferase"
                     /protein_id="HQX84725.1"
                     /translation="MVSVKAGDGVVLADIEGGQACEIAFCDETGRFDASALGARANSH
                     GEGLKAQLAEDSESTQRMKSALQRRNIDLGKAETVRLFGEDSQAGASAEFKV"
     assembly_gap    805..814
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <815..2925
                     /locus_tag="PKV23_04490"
     CDS             <815..2925
                     /locus_tag="PKV23_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013896789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1989 domain-containing protein"
                     /protein_id="HQX84726.1"
                     /translation="YNPLLRCIMRRTRAYLENTINPATGGYFLPKVTVRLFGEDSQAG
                     ASAEFKVSRDGVLIVAVPAAAMAPENQDTATDIELRITRSGKTNERSSMLPEPLADPV
                     QDIRIRASTASAYVVKAGEYIQVIDVSGRQCTDFQALSARKVDKGLDLALDATVTRTL
                     LGRSYPAPGLPSKSFDREFEPLVEIVQDTVGRHDAFALACTSRYYDDMGYPGHVNCTE
                     NFNHALAPYGVRPRKGWEALNYFYNTNIDHQNQLYLDEPWSRPGDYVLMRALTDLVCV
                     SSSCPDDIDAANGWDPTDIHIRTYAAKQKFSRAVVTRMTPDADAHLTKETAFHPRFSA
                     LTRNTTEYRGYWLPQRFNNDGPIEEYWACRENVTVTDLSPLRKFEVTGPDAEALLQYC
                     VTRDIRKLSVGQVVYTAMCYENGGVIDDGTVFRLGQNNFRWIGGDEFSGQWLRQQAEK
                     MGYKAWVRSSTDQLHNIAVQGPKSRDLLKEIIWTSPAQTAIGELEWFRFAVARIGHFE
                     GAPIVVSRTGYTGELGYEIFCHPKDALAVFDAVWEAGQKYGLKPMGLEALDMVRIEAG
                     LIFANYEFTSETDPFEAGIGFTVPLKSKSDDFIGKQALIQRKEHPRHRLVGLDIESNE
                     AVGHGDSVYMGRSKIGVVTSSTRSPVLGKNIALARVDVLHSEIGTGIEIGKLDGMQKR
                     LPGHIVRFAHYDPEKLRPRS"
     gene            complement(2922..>3267)
                     /locus_tag="PKV23_04495"
     CDS             complement(2922..>3267)
                     /locus_tag="PKV23_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010973723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HQX84727.1"
                     /translation="KTKVKAELAVWKGAYSIPAAADIVINATSIGLYPDVNARLDIAL
                     DSLRKGLVVADVIPNPPRTNLIKDAEARGCKTLDGLGMLVNQGVIGVKYWTGVDVDPA
                     VMKRKLEEIFSA"
BASE COUNT          675 a          927 c          997 g          658 t
ORIGIN      
        1 caatgttccc aaggaaggca gccagctctg gtttgacctg ctcgtcatcc ccaacaagtc
       61 gaaaaacaag gatggggcct atcagctcct caattacatg ctgaagccgg aagtggctgc
      121 cgcaaacacc aatttcctgc aatatgccaa tccgatactg acctcgacac cctacatcga
      181 gccaaaactc ctgggtgatc cggggcttta tccgccgaaa agcgtgctga aacggcttgc
      241 ggtgcagcca cccatggcgg cgaatgtcga ggccgagctc aagcggatct ggtccaagct
      301 gagcaaggag tagccgttcc gctgctttgg gcgtgggtgg cggtatatcg accatccgga
      361 ggaactgtgc gatattgaca gttcatttcc ctccggatgg tgacgtgtta tcccatgctt
      421 tcaactgcgc cacggcccag ccttatatat cgcccgggaa tccggacgtt accccttggg
      481 gttgaacgct atattgtgcg cggagcaggc tcgacggtgg tttcggtcaa ggccggcgat
      541 ggtgttgttc tggccgacat tgagggcggc caggcctgcg aaatcgcctt ttgcgacgaa
      601 acggggcgtt ttgatgcgtc ggcgctgggg gcacgcgcca acagccatgg cgaagggttg
      661 aaggcccagc ttgctgagga cagtgaaagc acacagcgca tgaaatcagc gctgcagcgc
      721 cgcaacattg atcttggcaa ggcagagacc gtgcgcctct ttggcgaaga ttcgcaggca
      781 ggggcgagtg ccgagttcaa ggtgnnnnnn nnnnagtaca atcccctgtt gcgctgcatc
      841 atgcgccgca ctcgggccta cctggagaac acgatcaacc ctgcgacggg cggctatttc
      901 ctgcccaagg ttacggtgcg cctctttggc gaagattcgc aggcaggggc gagtgccgag
      961 ttcaaggtgt cgcgcgacgg cgtacttatc gttgcagtgc ctgcggcggc aatggcgccg
     1021 gagaaccagg atacggccac ggatattgaa ttgcgcatta cccgcagcgg aaaaacaaat
     1081 gagcgcagca gtatgctacc ggaacctctc gccgatcccg tgcaggatat ccgcatcagg
     1141 gcctccaccg cttcggccta tgtggtgaag gctggtgaat atatccaggt catcgacgtc
     1201 tccggtcgtc agtgtacgga ttttcaggcc ctgtcagcgc gcaaggtcga caaggggctt
     1261 gacctggcgc tcgatgctac ggtcacccgc acattgctcg gccggagtta tccggcccct
     1321 ggccttccct ccaaatcttt tgaccgggag tttgagccgc tggtggaaat cgtccaggac
     1381 acggtgggca ggcatgacgc tttcgcgctc gcctgcacct cacgctatta cgatgacatg
     1441 ggctatcccg gccatgtgaa ctgtacggaa aatttcaatc atgcgctggc gccctacggc
     1501 gtgcgcccac gcaagggctg ggaagcgctg aattatttct acaacaccaa tatcgatcat
     1561 cagaaccagc tctatctcga cgagccatgg tcgcggcccg gcgattatgt gctgatgcgg
     1621 gcgctcaccg acctcgtctg cgtgtcgtcg tcctgtcccg atgatatcga cgcggccaac
     1681 ggttgggatc ccaccgacat tcacatccgc acctacgcgg ccaaacagaa attctcaaga
     1741 gcagtggtaa cccgcatgac tcccgacgca gacgcccacc tcaccaagga aaccgccttc
     1801 cacccgcggt tttcggccct gacccgcaac accacggaat accggggtta ctggcttccg
     1861 cagcgcttca acaacgacgg gcctattgaa gagtattggg cctgccgcga aaacgtgaca
     1921 gtcaccgacc tgtcgccgct gcgcaagttt gaagtgacgg ggcccgatgc ggaagcgctg
     1981 ctgcaatatt gcgtgacgcg tgacatccgc aagctgtcgg tggggcaggt ggtttatacg
     2041 gccatgtgtt acgaaaacgg cggggtgatc gacgacggca cggttttccg ccttggccag
     2101 aacaatttcc gctggatcgg cggggatgag ttcagcggcc aatggctgcg gcaacaggcg
     2161 gagaagatgg gctacaaggc ctgggtgcgc tcctcgacgg atcagctcca caacattgcg
     2221 gtccaggggc cgaagagccg cgacctgctc aaggaaatta tctggacctc gcccgcccag
     2281 acggccatcg gcgaattgga gtggttccgc tttgctgtgg cgcgtatcgg gcattttgag
     2341 ggagctccca tcgtcgtatc gcgtactggc tatacgggtg aactgggcta tgagatattc
     2401 tgccatccca aggatgcgct ggcggtcttc gatgcggtgt gggaggcggg gcagaaatat
     2461 ggcctgaagc ccatgggcct tgaggcgctg gacatggtgc gcatcgaggc gggtctgatt
     2521 ttcgccaatt atgagttcac gtcggagact gatccctttg aagcgggcat cggcttcacg
     2581 gtgccgctca agtccaagag cgatgatttc atcggcaagc aggcgcttat ccagcgcaag
     2641 gaacatccgc ggcacaggct agtggggctc gatatagaat caaatgaagc agtgggccat
     2701 ggcgacagcg tttatatggg acgttcaaaa atcggtgtgg tcaccagttc gacgcgctcg
     2761 cccgttctcg ggaagaacat tgcgctggcg cgggttgacg tgctgcacag tgagatcgga
     2821 accgggatcg aaatcggcaa gcttgacggc atgcagaagc gccttcccgg ccatatcgta
     2881 aggttcgccc actacgatcc ggaaaagctg cggccgcgga gctaggccga gaaaatttct
     2941 tcgagcttgc gcttcatgac ggcggggtcg acgtcgacgc cagtccagta tttgacgcca
     3001 atgacgccct ggttcaccag catgccgagg ccatcgaggg tcttgcatcc ccgcgcttct
     3061 gcatccttga tcaagttggt gcggggcgga ttgggaatga catcggccac caccagcccc
     3121 ttgcgcagac tgtccagcgc aatatcaagg cgggcattca catcgggata gaggccaatg
     3181 gatgttgcgt taatcacgat gtcggcggcg gcggggattg aataggcgcc tttccagacg
     3241 gcgagttcag ctttcacctt ggtcttg
//
LOCUS       DAORVN010000171         3265 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_25228_length_3265_cov_3.441433, whole genome shotgun sequence.
ACCESSION   DAORVN010000171 DAORVN010000000
VERSION     DAORVN010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3265
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25228_length_3265_cov_3.441433"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="PKV23_04500"
     CDS             complement(<1..252)
                     /locus_tag="PKV23_04500"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQX84728.1"
                     /translation="MKSMKLYTNVQRIHNELAALGIGPDAALRVDQLTPFDQYHYFGT
                     EAVDEALEVLQLQPGSRVLDVGAGIGGPARYIAAKTGVHV"
     gene            320..508
                     /locus_tag="PKV23_04505"
     CDS             320..508
                     /locus_tag="PKV23_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018701000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin transmembrane helix small protein"
                     /protein_id="HQX84729.1"
                     /translation="MEIFNYLVPLAVAAVAVVLLLGLWNMLRGGSASRSQNLMRWRVI
                     LQFAAILLIMAALWLANS"
     gene            563..901
                     /locus_tag="PKV23_04510"
     CDS             563..901
                     /locus_tag="PKV23_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013528816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HQX84730.1"
                     /translation="MSRTSNETRVTRDFWPKMKKSLASVPFAEDVVAAYYCAFDPATP
                     LKVKGILLGALAYFILPFDVIPDVLLGLGFTDDMAVLYAAISMIRSNMTQAHRDRAKD
                     TLEKLKAETV"
     gene            complement(898..1776)
                     /locus_tag="PKV23_04515"
     CDS             complement(898..1776)
                     /locus_tag="PKV23_04515"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HQX84731.1"
                     /translation="MKPAPNWIAFAPALFVVLWATGFVVARLSAGHVEPLTFLSIRFP
                     IAGALFVLIALYQRAVWPDRTHAFHASVAGALLHGGYLGPIYWAVAHGMPAGVSALIV
                     GLQPLMTAFLAAAIVGEKITARHWFGLVVGILGVGMVIWPKLTLDAVQGITPFTTALC
                     IFGALSVSLGTVYQKRFATGLHLASGGVWQYAGASVVVFSGAALSENFGFDGSFEAWL
                     ALGWSVIVLSLAAITLLMLLIRHGDVARVSGLIYLVPAVAAFSTYVVFGETLVPLQIL
                     GMAVCAAAVFIVTRRV"
     gene            1858..2742
                     /locus_tag="PKV23_04520"
     CDS             1858..2742
                     /locus_tag="PKV23_04520"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HQX84732.1"
                     /translation="MNPKQPTFVSLGLEALRRLFADEAIPLAGNIAFRTLFSIFPFLI
                     FLTALAGFFGNADLAEKVVSFLLSVAPEQLVHPLATEIRSILTVPRTGLLSIAALLTI
                     WSAMGGVDSIRVGLNRAYDLKENRNLFWLYGQSVLFVIGSAIGLLAIALLIVVAPVAL
                     AVLDRFAPDLRQDFAILNQLRYPVATIILIAGLQFCHRILPAKRLRSWDVLPGVLLTV
                     IVWIALASAFSAYLVNFNSFASTYASLSGLFAAMFFLYLAALVLILGGEVNRVLQAHR
                     ALHSLAGADPAGKDIKSP"
     gene            complement(2751..>3265)
                     /locus_tag="PKV23_04525"
     CDS             complement(2751..>3265)
                     /locus_tag="PKV23_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018700576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HQX84733.1"
                     /translation="INRKNFNCWGQMPKVGTGEYNEWLKAAREFGKAIWTFTGKGNSP
                     DIVFEHPGESTFPVSVLVVKRGGMVVICAGTTGYNLTMDARYLWMHQKRVQGSHFANL
                     MQASNANRLVMAQRIDPCMSDVYSWHDIPKAHMKMWKNEHRPGNMAVLVSAPRTGLKT
                     FEDVLEASAR"
BASE COUNT          662 a         1066 c          862 g          675 t
ORIGIN      
        1 gacatgtacc ccggtcttcg ccgcgatata acgcgccggt ccgccgatgc cggcccccac
       61 gtcaagaacc cgagaacccg gctgcagctg caaaacctcc agcgcctcat caaccgcctc
      121 ggtcccgaaa taatgatact ggtcaaaagg cgtgagctga tccacccgaa gtgcggcatc
      181 aggcccgatg ccaagggccg ccaactcgtt gtgaatgcgc tggacattgg tgtaaagttt
      241 cattgacttc atgagggatg gcgctccaaa acgccattat ctccccaaca cgcagcaaag
      301 ggcaacaggg ctacgcgcca tggaaatttt caattacctc gtgccgctcg ccgttgccgc
      361 tgtggccgta gtcttgcttc tcggtctgtg gaacatgctg cgcggcggct ccgccagccg
      421 ttcgcaaaac ctgatgcgct ggcgtgtgat tttgcagttt gccgccattc tcctcattat
      481 ggcagccctg tggctggcca acagttagcc attgaattgg tccccgtgaa ttcccatatg
      541 gcttttcgat tggagggaaa ccatgtctcg cacatccaat gaaaccaggg ttaccaggga
      601 cttctggccg aagatgaaga aatcactggc cagcgttcct ttcgctgaag atgtcgtagc
      661 cgcctattat tgcgcctttg atcccgccac gccgctcaag gtgaaaggca tcctgctcgg
      721 cgccctcgcc tatttcatcc tgccatttga cgtgatcccg gacgtccttt tgggccttgg
      781 ttttaccgac gatatggccg tgctttacgc cgcgatcagc atgatccgca gcaacatgac
      841 gcaagcccac cgtgaccggg cgaaggacac cctggaaaaa ctcaaggccg aaacggttta
      901 gacccgccgc gtgacaatga acaccgcagc cgcgcacacc gccatgccga gaatttgtaa
      961 aggaaccagc gtctcgccaa agaccacata ggtggagaac gctgccactg cgggaaccag
     1021 gtagatcaag ccggataccc gcgccacatc gccatggcgg atgagcagca tcagcagcgt
     1081 tatcgccgca agcgacagca caatcaccga ccagcccagc gccagccagg cctcaaagct
     1141 gccgtcaaag ccaaaattct cgctgagcgc cgcaccgctg aaaaccacaa ccgatgcacc
     1201 tgcatattgc cacactccgc cgctcgccag gtgcagcccc gtcgcaaaac gcttctggta
     1261 gaccgttccg agggaaacgc tgagcgcccc gaatatgcat agggcggtgg tgaacggcgt
     1321 gatgccttgc accgcatcca gtgtaagctt cggccagatc accataccta cacccaggat
     1381 tccaacaaca aggccaaacc aatggcgcgc cgtaatcttc tcgcccacga tggccgcagc
     1441 cagaaaggct gtcatcaatg gctgcaagcc cacgattagc gccgaaaccc ccgccggcat
     1501 gccatgcgcc accgcccagt agatcgggcc caggtagccc ccgtgcaaaa gggcgcccgc
     1561 gacagacgca tgaaaggcgt gggtccggtc cggccagaca gcccgctgat agagtgcaat
     1621 gagcacgaac agcgcccctg caataggaaa acggatgctg agaaaggtga gaggctccac
     1681 atgcccggcc gacagccgtg ccaccacaaa tccggtggcc cacaacacca cgaaaagcgc
     1741 gggtgcaaag gcgatccagt tgggcgcggg cttcatggca ttttccttgc caagctttat
     1801 ggccgcacgc gagtgttatc ttgagaaccg tcgcgcatat cggctatgcc taggtccatg
     1861 aacccaaagc agccgacctt cgtcagtctg ggcctcgaag cgctgcgccg tctctttgct
     1921 gacgaagcca ttcccctggc gggcaacatc gccttccgga cgctcttctc gatctttcca
     1981 ttcctgattt ttctgaccgc ccttgccggc ttttttggca acgcggactt ggccgagaag
     2041 gtcgtcagct tcctgctgag cgttgctccc gaacaactcg tgcacccatt ggccacggaa
     2101 atccgttcca ttcttacggt tccgcgcacc ggcctgctca gcatcgccgc gttgctcacc
     2161 atctggagcg ccatgggcgg tgtagacagc atccgcgtcg gcctgaaccg cgcctatgac
     2221 ctgaaggaaa atcgcaacct gttctggctc tacggccaaa gcgttctctt cgttatcggc
     2281 tccgccattg ggttgctggc cattgcgctg ttgattgtcg tggcgcctgt ggccctggcc
     2341 gtcctcgacc gtttcgcgcc cgatctgcgc caagacttcg ccatcctgaa ccagttgcgt
     2401 taccccgtcg ccaccatcat tctcatcgca ggtctgcagt tctgccaccg catcctgccg
     2461 gcaaaacgcc tcagaagctg ggatgttctg ccgggagtcc tgctgaccgt catcgtgtgg
     2521 attgcccttg caagcgcctt ctccgcttat ttggtgaatt tcaattcctt tgcctccacc
     2581 tatgcctccc tctccggcct ctttgctgca atgttctttc tctatcttgc ggcgctggtg
     2641 ctgatcctgg gcggcgaggt gaaccgcgtc ttgcaagcac accgcgcctt gcattcactt
     2701 gccggcgcgg accctgctgg caaggatatc aagtcacctt gactggttga tcatctcgca
     2761 gatgcttcaa gcacgtcttc aaatgttttc aatccggtgc gcggggccga caccaggaca
     2821 gccatgtttc ccggccggtg ctcgttcttc cacatcttca tgtgcgcctt gggaatgtca
     2881 tgccagctat agacatccga catgcagggg tcgatgcgct gcgccatgac caggcggttg
     2941 gcgttcgacg cctgcatcag attggcaaaa tgcgaaccct gcacccgctt ctggtgcatc
     3001 cacagatagc gcgcgtccat ggtgaggttg tagcccgtgg tgccggcgca gatcaccacc
     3061 atgccgccgc gcttcaccac cagcaccgaa accggaaaag tggattcgcc gggatgttcg
     3121 aacacgatat ccgggctgtt gcccttgccg gtgaatgtcc agatggcttt gccgaactcc
     3181 cgcgccgcct tcagccactc attgtactcc cccgtgccga ctttcggcat ctgaccccag
     3241 cagttgaaat tcttgcggtt gatca
//
LOCUS       DAORVN010000172         3264 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_25248_length_3264_cov_2.592085, whole genome shotgun sequence.
ACCESSION   DAORVN010000172 DAORVN010000000
VERSION     DAORVN010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3264
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25248_length_3264_cov_2.592085"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2104
                     /locus_tag="PKV23_04530"
     CDS             <1..2104
                     /locus_tag="PKV23_04530"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HQX84734.1"
                     /translation="EPAEAISGVVAVFWHANTPQHRYNSSIWYEGQGALDDERIFPPV
                     VRHIGDRVAAVVAESMEIAERAAKLIAVEYADLPAVFDPEESLTLCGPVTDETGEPSF
                     NNPIDDLSFGCGDTAAAFASADIIVESRITTPKTHHCAIETHACIAMPEPDGRILILS
                     PCQSIFAVQVVTAKALNMPADAIRVIKTPIGGSFGGKAEPILDPLCAFFAQRLKRPVL
                     IRYGRQETCIATRIRSRVIGRIRTALRGDGRILGRETETLVDVGAYCTGGNYLPGSML
                     QRLVRLYEVENENYRGRAVYTNTTPTGAFRGYGSPQIHTIAEINLDFAARKLKLDPVQ
                     LRLKNLVRPGSIEPYTQLDVGNARIRDCVKLGVKRFHWNEHRSRLPQSGRKRIGVGMA
                     CAAHINGCFPGFHEATTATLRLKPDNRIDLVCALHDLGCGANTTLAQIAAEVLTIPPS
                     QIDICAADTDICSYELGTRASRMTYICGEAIRLAAEALKARILNEAGLLLNCAVGDII
                     LEAGQLRRSYGPRETLSLAALAAQLRAQGDMLPEVTETYRAAANPSSYAAHFAGVEVD
                     TLTGLVCVRDYLAVHDIGRAINPMLVEGQIHGGVQIGIGYALYEDVDIDLKTGYMRGD
                     RFSRYHLANAPEMPPVDILLVEKGEPTGPFGAKAVGEIATIPVAAAVVNAVNHALGTS
                     LSDLPLTPDRIIAALEEL"
     gene            complement(2198..3259)
                     /locus_tag="PKV23_04535"
     CDS             complement(2198..3259)
                     /locus_tag="PKV23_04535"
                     /inference="COORDINATES: protein motif:HMM:NF015047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HQX84735.1"
                     /translation="MINAVTMGAGLPAEEDAQICADEGVYLDPIVSSAVALKVLLKRT
                     FRNWPDRYSDVIGAVVYEDPWLAGGHNGITSREDPDKPEAPYDRVKELRRIMNENKME
                     NVAIIMAGGVWYLRDWEHWLGNRDIGPIAFQFGTRDLLTRECPVTEEWKLLLLSMNEG
                     DVALNQFSPTGFYSSAYKNSMIKELYQRSERQVPFGRKCDSSMSEPVEGCGVPNNSYC
                     VTQQDKLKVDAWIAEGFNIPLRTPDNTLIFVTREAAREIKAYLTDCVGCLSTCKLSSW
                     SQSSEQKYTTGKRPDPRFHCIRKRLVEAIQGRDLDKQLIFAGHNAYKAKSDPFYRDEN
                     GHVFIPTVKQLVERLMTGN"
BASE COUNT          705 a          966 c          888 g          705 t
ORIGIN      
        1 ggagccggca gaagccattt cgggcgttgt cgctgtcttc tggcacgcca acacgccgca
       61 gcaccgctat aacagttcga tctggtatga agggcaggga gcgctcgatg atgaacggat
      121 cttcccgcca gtagtgcgcc acattggtga tcgcgttgcg gcggttgtag ccgaatccat
      181 ggaaatcgcg gaacgcgcgg cgaaacttat cgcggttgaa tacgccgacc tgccagccgt
      241 cttcgatccg gaagagagtc tcacgctatg cgggccagtg actgacgaaa caggagagcc
      301 ctcgttcaac aatcccattg acgatctgtc cttcgggtgc ggtgacacag cggctgcctt
      361 tgcgtcggcc gacatcatcg ttgaaagccg gatcactacg ccgaagactc atcactgtgc
      421 catcgaaacg catgcctgca ttgccatgcc ggagcccgac gggcgtatcc tgatcctgtc
      481 accatgccag agcatatttg cggtacaggt ggttaccgcc aaagctctca atatgccggc
      541 tgacgcaatt cgtgtgatca aaacgcccat tggcggttcg ttcggcggaa aggccgagcc
      601 cattcttgat cccctctgcg ccttctttgc tcaacgcctg aaacgcccag tgctgatccg
      661 ctacgggcgg caggaaactt gcatcgccac ccgcatccgg tcgcgggtga ttggccgcat
      721 ccgcaccgcc cttcgaggcg atggccgcat tcttggccga gaaaccgaaa cgctcgtgga
      781 tgtcggggct tactgcacag gcggcaacta tcttccgggc tccatgcttc agcgactagt
      841 tcgtctttac gaggtggaaa acgaaaatta ccggggccgg gcggtttaca ccaatacaac
      901 tccaaccggt gcattccgtg gttatggctc gccccaaatc cacaccatcg ccgaaatcaa
      961 cctcgatttc gccgcgcgaa agctgaaact ggacccagtg caactgcgcc tcaagaacct
     1021 tgtgcggcca ggttccattg aaccctacac gcagctcgat gtcggcaacg cgcgcatacg
     1081 cgactgcgtg aaactcggcg ttaagaggtt ccactggaac gagcaccggt cgcggcttcc
     1141 acaatcaggc cgcaaacgta ttggcgtggg catggcctgt gcggcccata ttaatggctg
     1201 ctttcccggt tttcacgagg ctacaaccgc aacattgcgg cttaagccag ataaccggat
     1261 cgatctcgtc tgtgcactgc atgatttggg ttgtggcgcc aatacgacgc tggcccagat
     1321 tgcagcggaa gtcttgacga tcccgccaag ccagatcgat atctgcgctg cggacactga
     1381 tatttgcagc tatgaactcg gcacccgtgc cagccggatg acatacatct gcggtgaagc
     1441 cattcggctg gcggcagaag cgctcaaagc ccggatacta aatgaggccg gccttctcct
     1501 gaattgcgca gttggcgaca tcatcctcga agcgggccag ttgcggcgca gctatgggcc
     1561 acgtgaaacc ctgtcattgg cagccctcgc ggcgcaactg agggcccagg gcgacatgct
     1621 gcctgaagtg accgaaacct atcgtgccgc ggcaaatccg agctcttatg ccgcccattt
     1681 cgccggtgtg gaagtcgaca cgctcactgg cctcgtatgc gtacgggact atcttgccgt
     1741 ccatgacatt gggcgtgcca taaatccgat gctggtcgag gggcagattc acgggggcgt
     1801 tcagattggt atcggctacg ccttgtatga agacgtcgac atcgacctga aaacgggata
     1861 catgcgcggc gaccggttca gccggtatca tctcgccaac gcgccggaaa tgccaccggt
     1921 tgatatcctt ctggttgaaa aaggtgagcc cactggaccc ttcggcgcga aggcggtcgg
     1981 cgaaatcgcc actattcccg tggccgccgc agtggtcaac gcagtaaacc atgctcttgg
     2041 gaccagcctc tccgacctgc cactcacgcc cgaccggatt atcgccgcac tcgaagaact
     2101 gtaagtccgc gcagcagctg acgatgtctc tgatttcgag gacatcatga aggacctgac
     2161 cttcgtaagg gcaactcgca aatgacctaa gaaatcatca attaccggtc atcaggcgtt
     2221 ccacaagctg cttcaccgtg ggaatgaaaa catggccgtt ttcgtcgcgg tagaacgggt
     2281 cactctttgc cttataggca ttatggccgg caaagataag ttgcttgtcc aggtcacggc
     2341 cctgaatagc ctccaccagc cttttccgaa tgcagtgaaa acgcggatct ggccttttgc
     2401 cagttgtata tttttgctcg gaactctggc tccaggagct gagtttgcag gtggatagac
     2461 agcccacgca atccgttaga tatgccttga tttcccgggc tgcctcgcgg gttacaaaaa
     2521 ttagcgtgtt atccggagtg cgcaagggaa tgttgaaccc ctcggcaatc caggcatcaa
     2581 ctttcaactt gtcttgttga gtgacgcaat agctgttgtt gggaaccccg cacccctcaa
     2641 caggttcgct catgctgcta tcgcacttac gcccaaaagg cacctgtctc tctgaacgct
     2701 ggtacaactc ctttatcatg ctgttcttgt acgccgatga ataaaaaccg gtggggctga
     2761 attggttaag ggctacatcg ccctcattca ttgagagaag cagaagcttc cattcttccg
     2821 tcacagggca ttcgcgcgtc aacagatccc gcgtaccaaa ctgaaaagca atggggccaa
     2881 tatcgcgatt gcccagccag tgttcccagt cccggaggta ccacacgcca ccggccatga
     2941 tgatggcgac attttccatt ttgttttcat tcatgatccg gcgcaattcc ttgacgcgat
     3001 catagggggc ttcaggcttg tcggggtctt cccgggaagt gatgccattg tggccgccgg
     3061 ccagccatgg atcctcataa acaactgcgc caataacgtc ggagtatctg tccggccaat
     3121 tcctgaaggt gcgcttcagc agcactttca gcgccacagc gctggaaaca atggggtcca
     3181 ggtacacccc ttcatcggcg caaatctggg cgtcttcctc cgcgggaagg cctgccccca
     3241 tggtcacggc atttatcatg ttgc
//
LOCUS       DAORVN010000173         3262 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_25274_length_3262_cov_2.786405, whole genome shotgun sequence.
ACCESSION   DAORVN010000173 DAORVN010000000
VERSION     DAORVN010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3262
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25274_length_3262_cov_2.786405"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..452
                     /locus_tag="PKV23_04540"
     CDS             <1..452
                     /locus_tag="PKV23_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006724338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HQX84736.1"
                     /translation="KIALGAGCNMVIASGHEDHPLRRIIEGERCTWFLAPLSALQSRK
                     RWIAGTLQPVGRLIVDDGARTALGKGKSLLPAGVRQVKGTFARGDTVSVVTLEGLEIA
                     RGLVAYDSGDAARIAGLKSADIEQTIGYRGRDEIIHRDDLVMTNGER"
     gene            457..1698
                     /locus_tag="PKV23_04545"
     CDS             457..1698
                     /locus_tag="PKV23_04545"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012110381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HQX84737.1"
                     /translation="MQELGLKARKAAGVLALASTQQKNAALMAMAKAIRANVNKLIAV
                     NASEVAAAKTKDLKDSFIDRLVLTTSRVEAMAKGLEDIAGLPDPVGTMLAEWTRPNGM
                     KISRIRVPIGVIGIIFESRPNVAADAGALCMKSGNAAILRGGSDGFQSSTLIVEALQQ
                     GLEAAGLPAAAIQMAPTTDRASVGEMLAGLGGTIDLIVPRGGKSLVARVQAEARVPVF
                     SHLEGICHVYVDKSANPAMARDIVLNAKMRRTGVCGSAETILIDRAGAKSNLSPIIRT
                     LIDSGCEVRGDAEAQAADARVKPASEADWSTEYLDAIVSLAVVNGVDEAMAHIAKYGS
                     QHTDAIITDDEEAAQRFLNEVDSAIVLHNASTQFADGGEFGFGAEIGIATGKLHARGP
                     VGLEQLTTFKYQVRGSGQIRS"
     gene            1695..2330
                     /locus_tag="PKV23_04550"
     CDS             1695..2330
                     /locus_tag="PKV23_04550"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000843.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HQX84738.1"
                     /translation="MMKLPLFGAGQRIGLFGGSFNPAHYGHYMVALYALKRLQLDWVW
                     WLVSPQNPLKDPGETGEYDERLKLTRRVAKHPRFVVTDLERQVNSRNTAETLLALNQA
                     MKQADFVWIMGADSLASLHKWHHWTDIVEALPLASLARPGYSIRALGSRAALRYAASR
                     VKTGEAAKLVTQKSPAWVFLPMPLRPESSTAIRNLNQPPRSPKQRATTRKG"
     gene            2659..3045
                     /gene="rsfS"
                     /locus_tag="PKV23_04555"
     CDS             2659..3045
                     /gene="rsfS"
                     /locus_tag="PKV23_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012493959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HQX84739.1"
                     /translation="MAPVRIETPKSKSYIDQSLLSIVMTQLEDAKAEDIIAINLDGKT
                     AIADNMVVASGRSNRHVGAIADQLVEKLKEAGHRHIRVEGMDQCDWVLVDAVDVVVHL
                     FRPEVRSFYNLEKLWSEHSPQEPLAG"
     gene            complement(3079..>3262)
                     /locus_tag="PKV23_04560"
     CDS             complement(3079..>3262)
                     /locus_tag="PKV23_04560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX84740.1"
                     /translation="KGKGADAASVTDAVLDELIASNPANPDLLKVKIKRILDAAAEGF
                     EAGLIVEAEGFFGPRF"
BASE COUNT          700 a         1061 c          925 g          576 t
ORIGIN      
        1 ggaaaattgc cttgggcgcc ggctgcaaca tggtcattgc ctccggccat gaggaccatc
       61 ccctcaggcg aattattgaa ggcgagcgct gcacctggtt tctggctccc ctgtcggcgc
      121 tccaatcaag gaaacgctgg atcgccggaa ccctgcagcc cgtgggccgc ttgatcgtcg
      181 atgacggcgc ccgcactgcc ttgggcaagg gtaaaagcct gcttcccgcc ggtgtcaggc
      241 aggtgaaggg aaccttcgct cgcggcgata cggtgagcgt agtgaccctt gaagggcttg
      301 aaatcgcccg cggccttgtc gcctatgact ctggcgatgc cgcaaggatt gcgggtctca
      361 agtccgctga catcgaacaa accattggct atagaggccg ggatgaaatc attcatcgtg
      421 atgacctggt gatgaccaat ggtgaacgct agcgccatgc aggagcttgg cctgaaagcc
      481 cgcaaggcag cgggcgtact ggcccttgcc tcaacccagc aaaaaaatgc cgccctgatg
      541 gccatggcga aagccatccg cgccaacgtc aacaaactca ttgccgtaaa cgcctccgaa
      601 gttgcagctg caaaaaccaa ggacttgaag gattcattca ttgaccgcct cgtccttaca
      661 accagccgcg tagaggccat ggccaagggc cttgaagaca tcgccggatt gcccgacccc
      721 gtcggaacca tgcttgccga atggacccgc cccaacggca tgaaaatttc ccgcatccgt
      781 gtgcccatcg gcgtcattgg gatcattttc gagagccgcc cgaatgtcgc cgccgatgcg
      841 ggagcgctct gcatgaagtc cggcaacgcc gccatcctgc gcggcggttc cgatggcttt
      901 caatcctcga ccctcattgt cgaagccctg cagcagggcc tcgaagcggc cggcctgccc
      961 gccgccgcaa ttcagatggc gccgaccacc gatcgcgcca gcgttggcga aatgcttgca
     1021 ggcctcggcg gcaccattga tttgatcgtg ccgcgcggcg gcaagagcct ggttgcccgt
     1081 gtgcaagcgg aagcgcgcgt tcccgttttc agccaccttg aaggcatctg ccatgtctac
     1141 gtcgataaat ccgccaatcc cgccatggcg cgcgacattg tcctcaatgc caagatgcgc
     1201 cgcaccggcg tctgcggctc cgccgaaacc attctcattg accgggccgg cgccaaatca
     1261 aacctctcgc cgatcatccg tacactgatc gattcgggct gcgaagtgcg cggcgatgcc
     1321 gaggcccagg ccgccgacgc ccgcgtgaag cccgcaagcg aagcggactg gtccaccgaa
     1381 tatctcgatg ccatcgtctc gcttgccgtt gtgaacggcg tggatgaggc tatggcccat
     1441 attgcaaaat atggctccca gcacaccgat gccatcatca ccgatgacga agaagccgcc
     1501 cagcgctttc tgaacgaggt cgacagcgcc attgtcctcc acaatgcctc cacccagttc
     1561 gccgatggcg gcgaattcgg cttcggcgcc gagatcggca tcgccaccgg caagctccat
     1621 gcccgcgggc ccgtgggcct ggaacagctc acgactttca agtaccaggt gcgtggctcc
     1681 gggcaaatcc gttcatgatg aagctgccgc tctttggcgc aggccagcgt atcgggctgt
     1741 ttggcggctc tttcaacccg gcccactatg gccattacat ggtcgcactc tacgccttga
     1801 agcgcctgca gctcgactgg gtgtggtggc tggtctcgcc gcaaaacccc ctgaaggatc
     1861 ccggtgaaac cggcgaatat gacgagcgcc tgaaactaac cagacgtgtg gcgaagcatc
     1921 cccgctttgt ggtgactgat ctggaacgcc aggtaaattc ccgcaatacc gccgaaaccc
     1981 tgctggctct gaatcaggcc atgaaacagg cagactttgt ctggatcatg ggggcggaca
     2041 gcctggccag tttgcacaaa tggcatcact ggaccgatat tgtggaagcc ctccccttgg
     2101 cctccctcgc ccgccccggc tactccatcc gtgccctcgg aagccgggcg gccctgcgct
     2161 atgccgcatc gcgcgtcaag accggcgagg ctgccaagct tgtcacgcaa aagtcaccgg
     2221 cctgggtttt cctccccatg ccactgcggc ccgaaagctc cacggcgatt agaaacttga
     2281 accagccgcc gcgaagccct aaacaacgcg ccacaacccg taaaggataa gaccctgaaa
     2341 aagcccagca agaagccttc cgccaaatcg aaatccgcga aagcagcaaa acccgcttcc
     2401 tcctccaagc gtccgcccaa gaccgccgct gaactcaaga ccatcatgat ggccaaggtt
     2461 cgtttcgccg aaaaggcgcg tgccggcctg cgcggcaaga ccacctccaa ggtgaagccc
     2521 aagggcaaga aaaaacttgc caagaaggta aagccggcgc cgaagaaaaa gcctgccgtc
     2581 aaggccaggg ccgcggtgaa gccaaaggtt gttgcaaagc ccaagaccgc gccaaaggca
     2641 aagcccgccg tcaaggccgt ggctccggtc aggatcgaaa caccaaagtc caagtcctac
     2701 attgatcaat cgctgctcag catcgtcatg acccagttgg aagatgccaa ggccgaggac
     2761 atcatcgcca tcaatctgga tggcaaaaca gccattgccg acaacatggt ggtggcctcg
     2821 ggccgttcca accgccatgt cggcgccatt gccgaccagc tcgtcgaaaa actcaaggaa
     2881 gcggggcacc gtcatatccg ggttgaaggc atggaccagt gcgactgggt gctggtcgat
     2941 gcggtcgatg ttgtcgtcca tctcttccgg ccggaagtgc gcagcttcta caatctcgaa
     3001 aagctctggt cggagcattc gccgcaagag cccctcgccg gataggccat gcggcttcag
     3061 gttctggcca tcggaaggtt aaaagcgggg cccgaaaaaa ccctcagcct cgactatcag
     3121 acccgcctcg aaaccctcgg ccgcggcgtc aagaatgcgc ttgatcttga ccttgagcag
     3181 gtcggggttc gcggggttcg aggcgatgag ctcgtccagc accgcgtcgg tcaccgacgc
     3241 ggcgtcggca cccttgccct tc
//
LOCUS       DAORVN010000174         3253 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_25421_length_3253_cov_2.937148, whole genome shotgun sequence.
ACCESSION   DAORVN010000174 DAORVN010000000
VERSION     DAORVN010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3253
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25421_length_3253_cov_2.937148"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..510
                     /locus_tag="PKV23_04565"
     CDS             <1..510
                     /locus_tag="PKV23_04565"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012385355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HQX84741.1"
                     /translation="VVISTGSLNFGDIVRAQDTGYGLLGWYWLPHLPMVALFFISALA
                     ETNRPPFDLPEAESELVAGYQVEYSSTPFLLFMAGEYIAIFLMCALTSLLFFGGWLSP
                     IPGIPDSPLWMVAKMGFFFFMFAMVKAIVPRYRYDQLMRLGWKVFLPLSLAWVVITAG
                     ALVAFGWLP"
     gene            507..743
                     /locus_tag="PKV23_04570"
     CDS             507..743
                     /locus_tag="PKV23_04570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX84742.1"
                     /translation="MMYNLFGALVFLVIGIIDVMFMQRMVYPQMRRRHEAAKVTGSQG
                     RDPALFMNGIRFFSLVVLPVVGFMLGGTFEAMFG"
     gene            751..1239
                     /gene="nuoI"
                     /locus_tag="PKV23_04575"
     CDS             751..1239
                     /gene="nuoI"
                     /locus_tag="PKV23_04575"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020185822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HQX84743.1"
                     /translation="MKLEQAARALFLKEFVSATWLALKYFVSPKATLNYPHEKGPLSP
                     RFRGEHALRRYPNGEERCIACKLCEAICPAQAITIEAGPRRNDGTRRTTRYDIDMVKC
                     IYCGFCQEACPVDAIVEGPNFEYATETREELLFNKEKLLANGDRWEREIAQNMALDAP
                     YR"
     gene            1255..1860
                     /locus_tag="PKV23_04580"
     CDS             1255..1860
                     /locus_tag="PKV23_04580"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012112151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HQX84744.1"
                     /translation="MVAALFFYLFASVAIAAAVMVISSRNPVHSVLFLILCFFNAAGL
                     FILLGAEFLAMILVVVYVGAVAVLFLFVVMMLDVDFVQLRQGMLNYLPVGGTIGLIVL
                     VELALVVGTWAISSDALTAAAAPSSGDVTNTEALGQILYTKYVYFFQAAGLVLLVAMI
                     GAIVLTLRHKQGVKRQDVSVQVARNPATAMEVVKVKPGQGI"
     gene            1863..2171
                     /gene="nuoK"
                     /locus_tag="PKV23_04585"
     CDS             1863..2171
                     /gene="nuoK"
                     /locus_tag="PKV23_04585"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011510679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HQX84745.1"
                     /translation="MTIGLSHYLTVAAILFTIGIFGIFLNRKNVIVIMMSIELMLLAV
                     NINLVAFSAALHDLVGQVFALLVLTVAAGEAAIGLAILVTYFRNRGSIAVEDINLIKG
                     "
     gene            2183..>3253
                     /locus_tag="PKV23_04590"
     CDS             2183..>3253
                     /locus_tag="PKV23_04590"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009736152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HQX84746.1"
                     /translation="MYQLIVFLPLIGALIAGLAGTKVFQNLGRDADVYGDHTAHAAHV
                     AHAHDDHHGHYDGPAWPMYLTTGLLCVSAVLSWIVFVGFLHEGHDAKVEVLRWVNSGA
                     FTANWMLRIDTLTAVMLVVVNTIAALVHVYSLGYMSHDEHQPRFFAYLSLFTFAMLML
                     VTSDNLLQMFFGWEGVGLMSYLLIGFWYTRESANAAAIKAFVVNRVGDFGFALGIFGC
                     FAVFQSIEFDTIFAAASTQTGKTIHFLSWEFDTLTTLCLLLFMGAMGKSAQFLLHTWL
                     PDAMEGPTPVSALIHAATMVTAGVFLVARLSPLYELAPHAKEVVVIVGAITAFFAATV
                     GLVQNDIKRVIAYSTCSQLGYMF"
BASE COUNT          534 a         1055 c          915 g          749 t
ORIGIN      
        1 gtcgtgatct ctacgggttc gctgaatttc ggcgatatcg tgcgggcgca ggacaccggc
       61 tatgggcttt tgggctggta ttggctgccc cacctgccga tggtggcgct gttcttcatc
      121 tcggcgctcg cggaaaccaa ccgcccgccg tttgacctgc cagaggcgga atccgaactc
      181 gtcgctggct atcaggtcga atattcctcg accccgttcc ttctgttcat ggcgggcgaa
      241 tatatcgcca tcttcctgat gtgcgcgctg acctcgctcc tcttcttcgg tggctggctc
      301 agccccatcc ccggcatccc ggacagcccc ttgtggatgg tcgcgaagat gggcttcttc
      361 ttcttcatgt tcgccatggt gaaggccatc gtgccgcgct accgctacga ccagctgatg
      421 cgcctgggct ggaaagtgtt tctgcccctg tcgctcgcct gggtggtgat caccgcaggc
      481 gccctggtgg ctttcgggtg gctgccatga tgtacaatct cttcggcgcc ctggtgtttc
      541 tggtgatcgg catcatcgat gtcatgttca tgcagcgcat ggtctatccg cagatgcggc
      601 ggcgccatga ggcagccaag gtgacgggct cgcaggggcg ggacccggcg ctgttcatga
      661 acgggatcag gtttttcagc cttgtcgtgc tgcctgtggt aggcttcatg ctgggcggca
      721 cttttgaggc aatgtttggg tgaatgaccg atgaaacttg aacaagccgc ccgcgccctt
      781 ttcctcaagg aatttgtttc agccacctgg ctggcgctga agtatttcgt gagccccaag
      841 gccaccctga actatcccca tgagaagggg ccgctcagcc cgcgcttccg cggcgagcat
      901 gcgctccgcc gctatcccaa tggcgaggaa cgctgcattg cctgcaagct gtgcgaagcc
      961 atctgcccgg ctcaggccat caccatcgaa gcgggcccgc gccgcaatga cggcacccgc
     1021 cgcaccaccc gctacgacat cgacatggtg aaatgcattt attgcggctt ctgccaggaa
     1081 gcctgcccgg tggacgccat cgtcgaaggc ccgaattttg agtacgccac ggaaacccgc
     1141 gaggagctcc tgttcaacaa ggaaaagctt ctcgccaacg gcgaccgctg ggaacgcgaa
     1201 attgcccaga acatggcgct tgacgcgcca taccggtgag ggagggacag taacatggta
     1261 gcagccttat tcttctatct cttcgccagc gtggccatcg ccgccgccgt gatggtcata
     1321 tcctcgcgca acccggtgca ttccgtgcta tttctcatcc tctgcttctt caatgccgcc
     1381 ggcctgttca ttcttctggg ggccgaattc ctcgccatga tcctggtcgt ggtctatgtc
     1441 ggtgccgtgg ccgtgctctt cctcttcgtg gtcatgatgc tggatgtcga tttcgtccag
     1501 ctccgccaag gcatgctgaa ttacctgccg gtgggcggca ccatcgggct catcgtcctg
     1561 gtggaactgg cgctggttgt cggcacctgg gccatttcgt cagacgccct gaccgccgcg
     1621 gctgcgccct cgtcgggcga tgtcaccaat acggaagcgc tgggccagat cctctacacc
     1681 aaatatgtct atttcttcca ggctgcgggc ctggtgcttc tcgtcgccat gatcggcgcc
     1741 atcgtgctga cgctgcgcca caagcagggc gtcaagcgcc aggacgtttc ggtgcaggtg
     1801 gcgcgcaatc cggcaaccgc catggaagtc gtcaaagtca aaccggggca ggggatctga
     1861 ccatgacaat cggcctttca cattatctga ccgttgccgc aattctcttt accattggca
     1921 ttttcggcat cttcctcaac cgcaagaacg tcattgtcat catgatgtcg atcgagctca
     1981 tgctgctggc tgtgaacatc aatcttgtgg ccttctcggc agcgctgcat gatcttgtgg
     2041 ggcaggtgtt cgccctgctt gttctgaccg tggctgccgg ggaagcggct atcggcctcg
     2101 ccattctcgt cacctacttc cgcaaccgcg gttccatcgc ggtggaagac atcaatctca
     2161 taaagggctg aagcgaaaac caatgtatca gctcatcgta tttcttcccc tgatcggcgc
     2221 gctcatcgcg gggctcgccg gcaccaaggt tttccagaac cttggccgcg acgcggatgt
     2281 ctatggcgat cacacggccc atgcggccca tgtggctcac gcccatgacg atcaccacgg
     2341 ccactatgac ggccctgcct ggcccatgta tctcaccacc gggctgcttt gcgtatccgc
     2401 cgtcctgtca tggatcgtgt ttgtcggctt tctccatgag gggcacgacg cgaaagtgga
     2461 agtcctgcgc tgggtgaatt ccggcgcctt caccgccaac tggatgctgc gcatcgacac
     2521 gctgaccgcc gtcatgctgg tggtggtgaa caccatcgcg gccctcgtgc atgtctattc
     2581 ccttggctac atgagccatg acgagcatca gccgcgcttc ttcgcctatc tctcgctctt
     2641 tacctttgcc atgctcatgc tggtcacctc cgacaacctc ctgcagatgt tcttcggctg
     2701 ggagggcgtg gggctcatgt cctatctgct catcggcttc tggtacaccc gtgaaagcgc
     2761 caatgccgcc gccatcaagg ccttcgtggt gaaccgtgtc ggcgacttcg gcttcgcgct
     2821 gggcatcttc ggctgctttg ccgtgttcca gtccattgaa ttcgacacca tcttcgccgc
     2881 cgccagcacc cagacgggca agaccatcca tttcctgagc tgggagtttg acacgctcac
     2941 cacgctctgc ctgctgctct tcatgggcgc catgggcaaa tcagcccagt tcctgctcca
     3001 cacctggctg ccggacgcca tggaaggccc aaccccggtc tcggccctga tccatgccgc
     3061 aaccatggtg accgccggcg tgttcctcgt ggcccgcctg tcgccgctct atgagctcgc
     3121 cccccacgcc aaggaagtcg tcgtcattgt gggcgccatc accgccttct tcgccgccac
     3181 cgtgggcctc gtgcagaacg acatcaagcg cgtcatcgcc tattcgacct gctcgcagct
     3241 cggctacatg ttc
//
LOCUS       DAORVN010000175         3237 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_25657_length_3237_cov_2.971716, whole genome shotgun sequence.
ACCESSION   DAORVN010000175 DAORVN010000000
VERSION     DAORVN010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3237
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25657_length_3237_cov_2.971716"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..941)
                     /locus_tag="PKV23_04595"
     CDS             complement(<1..941)
                     /locus_tag="PKV23_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014989278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HQX84747.1"
                     /translation="MRYDYASIGFYTFDCLGWPFTEVPPGGGTNFIDELTLAVSGAAG
                     TAAIAASKMGLKTLAVGGVGHDLMGDWVLKRLQHFGVDTSTMQQQQGWKTSSSIVTTR
                     RDGLRPALHMRGATADFYVDDAMMGQVTGAKVVHIGGIGLMRKMDQGRNAELMKRAKK
                     NGAVTTVDIFAGSKDDMPAVAMVLPYTDYFIPSIEEAQALTGLADMGDIARHFIDLGV
                     KCCVFTLGGEGAYYHHADGTVFRIPAFDIAVKCTCGCGDAFNAGFAVGLNKGFDPETT
                     VRFAQATSALNATGLGSQAGVESFEHTLNFMKTTKVRA"
     gene            complement(984..2198)
                     /locus_tag="PKV23_04600"
     CDS             complement(984..2198)
                     /locus_tag="PKV23_04600"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007590625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HQX84748.1"
                     /translation="MPIEVILPKVDMDMATGKIAKWHVKDGDAVRKGALLFEIETDKA
                     AMEIDAPGDGIIRNITAAEGAVIPVGQTVALIYQDGESAAAPAAPESLPPVKPITPAP
                     RPDAVPTAVLPAASASGRFPATPLARRLAKQGGLALENIAGSGPRGRISAADVQGALA
                     RPAAPQHAEPGSYQVIPLDGMRRTIAQRLTLSKQTVPHFYLTMNCDLTRLSEIRERLN
                     AHAPRDERKLPVWKLSINDFIIKAMASALRQVPDANVTWADEAIHKYHRCDVGVAVAV
                     EGGLFTPVVRAAESKSLSQISMEMKDLAARARARKLLPSEYQGGTTTISNLGMFGIEQ
                     FTAIINPPQATILAVGAGLERFVPVKGQPVLRTQMTCTLSCDHRAVDGALGAQLLAAF
                     KTFIEEPALMLA"
     gene            complement(2202..3200)
                     /locus_tag="PKV23_04605"
     CDS             complement(2202..3200)
                     /locus_tag="PKV23_04605"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010911829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HQX84749.1"
                     /translation="MNAPLRELTYAQAIQEALATAMEADKRVFIMGEDIGVYGGAFQV
                     TGDLVHRFGEDRVMDTPISELGAAGIAVGAALAGSRPILEFQFSDFATLAMEQIVNQA
                     AKMRFMLGGKVSVPLVMRLPSGSGTGAAAQHSQSLEAWLAHVPGLKVVQPSTPHDAKG
                     LLLAAIDDPDPVMVFEHKLLYKMKGEVPEDAYRVPIGKAAVRREGRHATIVATSIMVH
                     RALEAAEVLAGEGIEVEVIDLRTLRPLDTATIIESVKKTTRLACVYEGVKTLGIGAEI
                     SALIAESEAFDYLDAPILRLGGVDSPIPYNPVLEKAAVPQTPGIIEQVRNLVKGRI"
BASE COUNT          641 a          963 c          985 g          648 t
ORIGIN      
        1 gctctaacct tcgtcgtctt catgaagttc agcgtgtgct cgaaggattc gacgccggcc
       61 tgcgagccga gaccggttgc gttaagggct gaagtcgcct gggcaaagcg cacggttgtt
      121 tcgggatcaa aacccttgtt gagcccaacg gcaaagcccg cattgaaggc atcgccgcag
      181 ccgcaggtgc atttcaccgc gatatcgaag gccggaatcc tgaaaacggt gccatcggca
      241 tgatgataat aggcgccctc gccgcccagc gtaaacacgc agcacttcac gccgagatca
      301 ataaaatgcc tggcgatgtc ccccatgtcc gcaaggccgg tcagggcctg ggcttcctct
      361 atggagggaa tgaaatagtc ggtgtaaggc agcaccatgg ccacggcggg catgtcgtcc
      421 ttcgaaccgg caaagatatc caccgtcgtc accgcgccat tcttcttggc gcgcttcatc
      481 agctcggcat tgcggccctg gtccattttc cgcatcaggc caatgccgcc gatatgcacc
      541 accttggcgc cggttacctg gcccatcatg gcgtcatcga catagaagtc cgcggttgct
      601 cctctcatgt gcagcgcggg gcgcaagcca tcccggcgtg tggtgacgat tgacgaggac
      661 gtcttccagc cctgctgctg ttgcatggtg gaggtatcga cgccgaagtg ctgcagccgc
      721 ttcagcaccc agtcgcccat caggtcatgg cccaccccac caacggccag ggttttcaga
      781 cccatcttcg aagcggcgat tgccgcagtt ccggccgctc cggacacggc cagggtgagt
      841 tcatcgatga aatttgtgcc gccgcccggc ggaacttccg tgaacggcca gcccaggcag
      901 tcgaaagtgt agaagccaat cgacgcgtaa tcatagcgca tgggttttcg tctccgttca
      961 aagaaacaat actattgcat gggtcaggcc agcatcaagg ccggttcttc gatgaaagtt
     1021 ttgaatgctg ccagcaattg cgcgccaagc gccccgtcca cggcacggtg atcgcaggag
     1081 agggtgcagg tcatctgggt gcgcaatacc ggctgacctt tcacgggcac gaagcgctcc
     1141 aagccggcgc caacggcgag gattgtcgcc tggggcggat tgatgatggc ggtgaactgc
     1201 tcaatgccaa acatgccgag attggaaatg gtcgtcgtgc ccccctggta ttccgaaggc
     1261 aacaatttgc gggcgcgggc acgggcggcc agatccttca tctccatcga aatctgtgac
     1321 agtgacttgc tctcggcagc ccttacgacc ggggtaaaca agcctccctc cacggcaacg
     1381 gccacgccta catcgcatct gtgatacttg tgaatggcct cgtcggccca tgtaacattg
     1441 gcatcgggca cctgcctgag ggccgaggcc atggccttga tgatgaaatc attgatcgac
     1501 agtttccata ccgggagttt cctctcgtcg cgcggtgcgt gggcgttgag ccgttcacga
     1561 atttccgaaa gccttgtcag gtcgcagttc atggtgagat agaaatgcgg aaccgtctgc
     1621 ttggacagcg tcaggcgctg ggcgatggtg cggcgcatcc catccagtgg aatcacttga
     1681 tagctgccgg gctccgcatg ctgcggggct gccggcctgg caagagcacc ctgaacgtcg
     1741 gctgctgata tgcgcccgcg cggaccgctt ccggcgatat tctcaagggc caatccgccc
     1801 tgtttcgcca gccgccgggc aagcggggtt gcaggaaaac gtccagatgc ggaggctgca
     1861 gggagaacag ctgttggtac cgcatctgga cgaggtgccg gtgtaatggg cttcaccggg
     1921 ggtaaggatt ccggagcggc aggtgccgca gcggactcgc cgtcctgata gataagagcc
     1981 acggtttgtc cgacggggat taccgcccct tcggcggcgg tgatgttgcg gatgatgccg
     2041 tcgcctggcg catcgatctc catcgccgcc ttgtcggttt caatctcaaa gagcaatgcg
     2101 cctttcctga cggcatcgcc atccttcaca tgccatttgg caatcttgcc ggtggccatg
     2161 tccatgtcga ccttgggcag tatgacctcg atgggcatgg gtcaaatcct gcccttcacg
     2221 agattgcgga cctgctcgat gatcccgggg gtttgcggca cggcggcttt ttccagaacc
     2281 ggattgtagg gaatgggcga gtcaacgccg ccaagcctga ggattggcgc gtcgagataa
     2341 tcaaaggctt cgctttcggc aatgagtgcg ctgatctcgg cgcctattcc caaggtcttc
     2401 accccttcat agacgcacgc cagccgggtt gtcttcttga cgctttcgat gatggtggcg
     2461 gtatcgaggg ggcgcaaggt acggaggtca atgacctcaa cctcaatgcc ctcgcccgcc
     2521 aatacttcgg cagcttcgag agctcggtgc accatgatgg aggtggcaac aatcgtggca
     2581 tggcggcctt cgcgccggac ggcggccttg ccgatgggaa cgcggtaagc atcctcgggc
     2641 acttcgccct tcattttgta gagcagcttg tgctcaaaaa ccatcaccgg atcgggatcg
     2701 tcgatggccg cgaggagcaa gcccttggcg tcatgcgggg tcgagggctg gacaactttc
     2761 aggcccggaa catgagcaag ccaggcttcc aacgattggc tgtgctgggc ggcggctccg
     2821 gtgccagagc cggacggcaa acgcatgacg agcggcaccg aaaccttgcc gccaagcatg
     2881 aaacgcattt ttgccgcctg gttgacgatc tgctccatgg caagcgtggc aaagtctgaa
     2941 aactgaaatt ccaggatcgg ccggctgccg gcaagggccg caccaacggc aattcccgcc
     3001 gcgccaagtt cggaaatcgg cgtgtccatg acacgatcct caccgaagcg gtggacaaga
     3061 tcgccggtga cctgaaaagc cccgccataa acaccgatgt cttcgcccat gatgaagaca
     3121 cgcttgtcag cctccatggc agtggccagc gcttcctgga tggcttgcgc gtaggtcagt
     3181 tcgcggaggg gcgcgttcat gaaggatttc ctggcgtata gacaccgcgc gtcacat
//
LOCUS       DAORVN010000176         3230 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_25740_length_3230_cov_3.388346, whole genome shotgun sequence.
ACCESSION   DAORVN010000176 DAORVN010000000
VERSION     DAORVN010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3230
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25740_length_3230_cov_3.388346"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..510)
                     /locus_tag="PKV23_04610"
     CDS             complement(<1..510)
                     /locus_tag="PKV23_04610"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006469300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HQX84750.1"
                     /translation="MPVQTIVSAVTEKIIERSRPTRSRYLSRIAAAASAQPKRRALGC
                     ANLAHGFAACGPHDKNELRNGAGPNLGIVTSYNDMLSAHQPFEHYPQIIRDAARAAGG
                     TAQVAGSVPAMCDGVTQGETGMELSLFSRDVIALSTAVALSHQMFDAAVYLGVCDKIA
                     PGLLLGALAF"
     gene            complement(525..1181)
                     /locus_tag="PKV23_04615"
     CDS             complement(525..1181)
                     /locus_tag="PKV23_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003527053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HQX84751.1"
                     /translation="MIAKERLFESRGSLSSALATDVANALQHHVRTKGSSCLAVSGGL
                     TPKLFFETLSRFDVPWSRVTITLVDERQVPETDPRSNARLVRENLLQNSAAGAQFVPL
                     FDNPEAENVGTLDVVVLGMGNDGHTASFFPGGDNLGEALNPKTGKRIVTMSAPAAGEP
                     RLTFTLPALLDASLLCLHIEGQEKRDVLNKALGEGPAEAMPVRAVLRSKKPLTLYWCP
                     "
     gene            complement(1178..2659)
                     /gene="zwf"
                     /locus_tag="PKV23_04620"
     CDS             complement(1178..2659)
                     /gene="zwf"
                     /locus_tag="PKV23_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008196915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HQX84752.1"
                     /translation="MTPRTIPVDVFDLIVFGATGDLAQRKLLPALFHRDVQGQIPPSA
                     RIIGSSRRAMTREAFRAFALASIETHVSEDERKPDTCKRFLDRLDYVAAEASQESGWK
                     DLEKVLKPHADRIRVFYLAVGPDLFGPICERLGGHNLVTPNSRVVVEKPLGKNGASAK
                     ALNEQIGRIFPEPSIYRIDHYLGKETVQNLMALRFANALFEPVWNAAHIDNVQITVAE
                     TLGVEGRAGYYDTAGALRDMVQNHMLQLLCLVAMEPPTSIEADSVRDEKLKILQSLKP
                     LTDGTLASQIVRGQYRAGASAGGAVPGYLEELGSDISKTETYVALKAEIGSWRWNGVP
                     FYLRSGKRLPSRLSEIVVEFKPVPYSVFGTSAGNIASNTLVIRLQPDEGVKLMLMIKD
                     PGPGGMRLRQVPLDMSFADAFGVRNPDAYERLLMDVVRGNQTLFMRRDEVTAAWNWID
                     PILDYWRESPELPKPYTAGTWGPTAAVALIERDGRTWHEGEVA"
     gene            2812..>3230
                     /locus_tag="PKV23_04625"
     CDS             2812..>3230
                     /locus_tag="PKV23_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006727976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HQX84753.1"
                     /translation="MIVCCGEALIDMLPRTSKEGAAVYQPLNGGSIFNTAIALGRLGI
                     ITGFFSGLSTDFFGESLVAGLKASNVDLKYTKIWDRPSTLAFVKLDNGQARYSFFDEN
                     SASRMLTTKDLPKLAADVNALHFGSISLIPEPGGSTL"
BASE COUNT          668 a          929 c          992 g          641 t
ORIGIN      
        1 gaacgcgagc gcaccgagca gcagtcccgg cgcgatcttg tcgcagacac ccaggtaaac
       61 agcagcatca aacatctggt gggacaatgc cacggcggtt gaaagggcaa tcacgtcgcg
      121 cgagaacagc gagagttcca tgccggtttc tccctgggtg acgccatcgc acatggcggg
      181 aacgctgcct gccacctggg ccgtgccgcc cgccgcacga gctgcatcac ggatgatctg
      241 cgggtaatgc tcaaacggct ggtgggctga cagcatgtca ttgtaggagg tgacgatgcc
      301 gaggttcggg ccggcgccat tgcgcagttc attcttgtcg tgggggccgc aggcggcaaa
      361 gccatgggca agattggcgc agcccagcgc cctgcgtttg ggttgcgcac tggcggccgc
      421 cgcaatgcgg ctgagatagc ggctgcgcgt tggcctgctg cgctcgatga tcttctcggt
      481 gaccgcagag acgattgtct ggaccggcat ggatttcaac tccgtcaggg acaccaataa
      541 agtgtcagag gtttttttga ccgcagaacc gcccggaccg gcatggcctc agccgggcct
      601 tcgccaaggg ctttgtttag cacgtcgcgc ttttcctgtc cttcaatgtg aaggcagaga
      661 aggctcgcgt cgaggagggc tggaagggtg aaggtcaggc ggggctcacc ggcggcaggg
      721 gccgacatcg tcactatgcg cttcccggtc tttggattga gcgcctcacc caaattatcg
      781 ccaccgggga agaatgaggc cgtgtggcca tcattcccca tgccgaggac tacgacatcc
      841 agggtgccga cattttccgc ttcgggatta tcaaatagcg ggacaaactg cgcaccagca
      901 gcgctattct gcagcaggtt ctcccgcacc agcctggcgt tcgagcgggg atctgtctcg
      961 ggcacctggc gctcgtccac cagggtaatg gtcaccctgc tccagggaac atcaaatcgc
     1021 gacagggttt caaagaagag cttgggcgtc aatccacccg aaaccgcaag acaggaactg
     1081 cccttggttc tcacatgatg ctgcagggcg ttggcgacat cggtggcgag ggcggatgac
     1141 aggctgcccc ggctttcaaa caaccgctcc ttggcgatca tgcaacttcg ccctcatgcc
     1201 aggtgcgccc gtcgcgttcg atcagggcga cggcggcggt gggaccccag gtgccggccg
     1261 tgtaaggttt cggcagttcg ggagattccc gccagtaatc cagaatggga tcgatccagt
     1321 tccatgcggc agtcacttca tcgcggcgca tgaagagagt ctggttgccg cgcaccacat
     1381 ccatgagcag gcgctcgtag gcatcgggat ttctgacgcc gaaggcatcg gcaaagctca
     1441 tgtcgagggg cacctggcgc aggcgcatgc cgccggggcc agggtccttg atcatcagca
     1501 tgagcttcac cccttcatcg ggctgcaggc ggattaccag cgtgttggaa gcaatgttac
     1561 cggcgcttgt tccaaatacc gaatacggca cgggcttgaa ttcaacgacg atttccgaaa
     1621 gacgcgaggg aagccgtttg ccggaacgca gatagaaggg aacgccattc caacgccagc
     1681 ttccgatctc ggccttgagc gcaacatagg tttcggtctt ggagatatcc gagcccagtt
     1741 cttcgaggta gcctggaacc gcgcctccgg ctgaagcgcc ggcgcgatat tgcccgcgca
     1801 cgatctggct ggcgagggtt ccatcggtca gcggcttgag cgattgcaga attttcaact
     1861 tttcatcgcg cacggaatcg gcttcaatcg atgtgggcgg ctccatcgct accaggcaga
     1921 gaagctgcag catgtggttc tgcaccatgt cgcgcaacgc gccagccgtg tcatagtaac
     1981 cggcgcggcc ctctacgcct aaggtttccg ccacggtaat ctgcacattg tcaatgtggg
     2041 cggcgttcca gacgggctca aacagggcgt tggcgaagcg cagcgccatg aggttctgca
     2101 cggtttcctt gccgagataa tgatcgatac ggtagatcga cggttccgga aaaatccggc
     2161 cgatctgctc gttcagggcc ttggccgagg cgccattttt gcccagtggc ttttccacca
     2221 caacacggga attcggcgtc accagattgt ggccgccgag acgctcacaa atgggcccga
     2281 acagatcagg ccccacggcc aggtaaaaga cgcggatgcg gtcggcgtgg ggcttaagga
     2341 ctttttcaag atccttccag ccggattcct ggcttgcttc ggcggcgaca taatccaggc
     2401 ggtcgagaaa ccgcttgcag gtgtcgggct tgcgctcatc ttcagacaca tgggtttcaa
     2461 tagaggcgag agcaaaagcg cgaaacgcct cacgggtcat ggcgcggcgg gaagagccga
     2521 taatccgggc cgacggggga atctggccct gcacatcccg gtgaaaaagg gcaggcagca
     2581 gcttacgctg ggccaggtca ccggtggcgc caaagacgat aagatcgaag acgtcgacag
     2641 gaatagttct gggggtcata ttcggttgag attccggttt tgaggtacgc caatcagagt
     2701 ggcgatctac acgccgaaag ctaattatgc aataattcaa ttccactgat ttattgattt
     2761 ttttgccgac tccggcaaag gatagctacg gtaccccaga aggagaatta catgatcgtt
     2821 tgctgcggcg aagcgctgat cgacatgctg ccgcgcacgt ccaaggaggg tgcggcggtt
     2881 taccagcctc ttaatggcgg ctcgattttc aacacggcca ttgccctggg gcggctggga
     2941 atcatcaccg gatttttcag cgggctgtcc acggacttct tcggcgagag cctggttgcc
     3001 ggcttgaagg ccagcaatgt cgatctgaaa tacaccaaga tatgggacag gccttcaacc
     3061 cttgcctttg tgaagctgga caacggccag gcgcgctatt ccttcttcga tgaaaattcc
     3121 gccagccgga tgctgaccac caaagacctt ccaaagcttg ccgctgacgt caacgccctg
     3181 catttcggct ccatcagcct cattcccgaa ccgggcgggt cgacactgga
//
LOCUS       DAORVN010000177         3205 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_26088_length_3205_cov_3.201270, whole genome shotgun sequence.
ACCESSION   DAORVN010000177 DAORVN010000000
VERSION     DAORVN010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3205
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26088_length_3205_cov_3.201270"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..286)
                     /locus_tag="PKV23_04630"
     CDS             complement(<1..286)
                     /locus_tag="PKV23_04630"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HQX84754.1"
                     /translation="MFRDRTDAANRLVEKLTPYRNKNPLILAIPRGAVPMAKIIADKL
                     GGELDVVLVRKLRAPHQPELAIGSVDESGWAYVAGFAAAYGATEDYIEKEK"
     gene            570..710
                     /locus_tag="PKV23_04635"
     CDS             570..710
                     /locus_tag="PKV23_04635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX84755.1"
                     /translation="MAREKAIEVANALLAEGIEEGKAIRIAIAAAKGWAEHHAFPVHD
                     DE"
     gene            730..3153
                     /locus_tag="PKV23_04640"
     CDS             730..3153
                     /locus_tag="PKV23_04640"
                     /inference="COORDINATES: protein motif:HMM:NF025038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQX84756.1"
                     /translation="MAISRVNPADLRVSIDPASLFFTDTSELLEYPVPWIGQERAEMA
                     ARFGLGIDQPGYNLFVLGEVGSGRSTLLREMMQTVAVNRQVPPDLCYLHNFDAPERPR
                     ALRLPAGQGRLLRQLMTQMAKNLQSEIPKRLDGQDFKADSERIKKTYSTEETRAYAEL
                     DAFAEARSFTLYREAGRLVFTLRDEKGRPYTESEAIALSKERRAAIDKAELELRAEIT
                     RFMDKMQPLERVMNEGLAALQRQFVKPLLDHAMQEIRVGLKKQIKDSVKLNAYLEQAA
                     HDVLDNLAVFQVTEDDEEARLEALSSVLSRLHVNLIVDNSDVNGAPVIVEDNPVFRSL
                     FGSIEYQSDNEVLVTDFSRIRAGSLLKAHGGYIMLHLRDLITDELVWEKLRRLLRSGR
                     LQIEESGLSLSPIATVSLVPEAVDVEVKIILIGSSENYYELQEADPEFARHFRVKVDF
                     AESFPSNAETRQACAVFVAHVCRKTGLPHFSAAGVARLLENSHREADDQSRQSAIFAQ
                     TEALVLESATLCRSRGATLVEALDVEAALRAKAMRHDYPEQQLQESIADGERLIALSG
                     EKVGQLNGLTQIDLGDYRFGFPVRVTARTFAGDEGLLNIEREVEMSGPIHDKGVLILS
                     SYLSALFAHIAPLALNASISFEQEYGGVEGDSASCAEFYVLLSSLSGLPLRQGIAVTG
                     AVNQHGEILPVGGINEKIEGYFRTCEAAGLDGSQGVLIPHRNRRHLMLAQKVVEAVSR
                     GQFHIHTAEHVCEGIELLTGVSSGMADKEGAYPRSSVLGLAQKTLQAYRRACQEAEVG
                     PKPARGRSR"
BASE COUNT          684 a          883 c          984 g          654 t
ORIGIN      
        1 gcttctcctt ttcaatgtag tcttcagtgg caccataggc ggcggcgaac ccggcaacat
       61 aggcccagcc actctcgtca acagagccga tggccagttc aggctgatgc ggggcacgca
      121 gtttgcgcac caggacaaca tccagctcac cacccagctt gtccgcgatg attttcgcca
      181 tcggaaccgc tccgcgggga atggcgagaa tcaacggatt cttgtttcga tagggagtca
      241 gtttctcgac caggcggttg gctgcatcgg tgcgatcacg aaacatgtct tttttccctt
      301 catggaacgg gtcagcgaaa ccaaaacacc taccttggtt ggcagaatag agggaatgcc
      361 gaaagccgca ttgacatacc gcaagctcgc attcgcagac acgggaaaaa gggtcttttg
      421 cgaattgtca aagcaggaac ccagcctcac ttttacattg aaaccatgcc gttggttcgg
      481 aaggattggc gggtcgattt tttttgggga gtccggctgt gccatggaat gaaaaacgct
      541 tccccccgtc catgcaagat ctcagtgaag tggctagaga gaaagctatc gaggtcgcca
      601 atgcgttgct ggctgaaggc atagaagaag gcaaggccat ccggatcgcc atcgccgccg
      661 ccaagggctg ggcggagcac catgcattcc cggtgcatga tgacgagtga taaccggttt
      721 cagactgaca tggccatttc cagagtaaat cccgctgacc ttcgcgtcag catcgatccg
      781 gcatccctgt tctttaccga tacctctgaa ttactcgagt atccggttcc atggattggg
      841 caggagcgcg ccgaaatggc ggcacgcttt ggcctcggca ttgaccagcc gggctacaac
      901 ctgttcgtgc tgggggaagt gggcagcggt agatctaccc tgttgcggga gatgatgcag
      961 acggtggcgg tgaaccggca agtgccgcct gatctgtgct acctgcacaa cttcgatgcg
     1021 cccgaacggc cacgggcgtt gcgcctgcct gcaggacagg gccgcctgct gcgtcagctc
     1081 atgacacaga tggcgaagaa cctgcagtca gaaattccga agcgtctgga cgggcaggat
     1141 ttcaaggcag acagcgaacg gatcaagaaa acctacagca ccgaggaaac cagggcctac
     1201 gcagagctgg atgcctttgc ggaagcgcgc agcttcaccc tttaccgcga ggcggggcga
     1261 cttgttttca ccttgcgtga tgaaaaaggc cgtccctata cggaaagcga agccattgcc
     1321 ttgtcgaagg aacgtcgcgc cgcgattgac aaggcggaac tggaactgcg cgccgagatt
     1381 acccgtttca tggacaagat gcagcccctg gagcgcgtca tgaatgaggg gctggcggcg
     1441 ctgcaacgcc agtttgtcaa accgctgctg gatcatgcga tgcaggaaat ccgcgtgggc
     1501 ctgaagaagc agatcaagga ttctgtcaag ctcaatgcct atctggaaca agccgcgcat
     1561 gacgtattgg acaacctggc tgtgtttcag gttaccgaag atgacgaaga ggcccggctg
     1621 gaggcgctga gtagcgtgtt gtcgcgcctg catgtcaatc tgattgtgga caacagcgat
     1681 gtgaacggtg cgcccgtgat cgttgaggac aacccggtgt tccgctccct gttcggcagc
     1741 atcgaatacc agtccgacaa tgaagttctg gtgacggatt tctcgcgtat tcgtgccggc
     1801 agcctgctga aggcacatgg cggctacatc atgctgcatc ttcgcgacct gatcaccgat
     1861 gaactggttt gggaaaagct caggcgcctt ttgcgaagtg gtcgactgca aatcgaggag
     1921 tccggcctgt ccctttcacc gattgccacc gtgtcgctgg tgccagaggc cgtggatgtt
     1981 gaagtgaaga tcatcctgat cggctccagc gagaactact atgaattgca ggaagcggac
     2041 ccggagtttg cgcggcactt tcgggtcaag gtggattttg cagagagctt cccgtccaac
     2101 gccgaaacgc ggcaggcctg tgccgttttt gtggcccatg tctgcagaaa aacagggttg
     2161 ccccattttt cagccgctgg cgtggcacgc ctgctggaaa attcccaccg ggaggcagac
     2221 gatcagtccc gccagagcgc gattttcgcg cagaccgagg cactggttct ggaaagtgcc
     2281 accctgtgcc gcagtcgcgg ggcaaccctg gtagaggcac tggatgtcga ggccgctctg
     2341 cgcgccaagg ccatgcggca tgactacccc gagcagcagt tgcaggagtc catcgccgat
     2401 ggcgagaggc tgattgcact ttctggcgaa aaagtggggc aactcaacgg gctgacacag
     2461 attgacctgg gcgactaccg cttcgggttt ccggtgcgtg tgaccgcgcg gacttttgcc
     2521 ggcgatgaag gtctgctcaa cattgagcgc gaagtggaga tgtcgggccc gattcacgac
     2581 aagggcgtgc tcatcctgag cagctacctg tcagcactgt ttgcccacat tgcaccgctg
     2641 gcattgaatg catccatttc gttcgaacag gaatatggcg gcgtggaagg agactcggcc
     2701 tcgtgcgcgg agttttatgt cttgctgtct tccctgtccg gcctgccctt gcggcagggg
     2761 atcgccgtga ccggggcggt caaccagcat ggcgagattt tgccggtagg cggcatcaac
     2821 gaaaagatag agggttactt tcgcacctgt gaagccgccg ggcttgacgg cagtcagggc
     2881 gtgctgattc cgcaccgaaa ccgccgtcat ctgatgctcg cgcagaaggt cgtcgaagcg
     2941 gtctccaggg gtcagtttca tatccatacc gccgagcatg tgtgcgaagg tatcgagttg
     3001 ctgaccggtg tgtcttcagg tatggcggac aaagaaggcg cctacccgcg tagcagcgta
     3061 ttgggcctgg cccagaagac cctgcaagcc tatcgtcggg catgccagga ggccgaggtg
     3121 ggccccaaac ccgctcgcgg gcgttcacgt tgacaggcag ggagcaaaca tgcctcgaac
     3181 agggcgtctg gcattggcac ttctc
//
LOCUS       DAORVN010000178         3192 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_26286_length_3192_cov_2.936564, whole genome shotgun sequence.
ACCESSION   DAORVN010000178 DAORVN010000000
VERSION     DAORVN010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3192
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26286_length_3192_cov_2.936564"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            130..618
                     /locus_tag="PKV23_04645"
     CDS             130..618
                     /locus_tag="PKV23_04645"
                     /inference="COORDINATES: protein motif:HMM:NF016221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HQX84757.1"
                     /translation="MRKLVLEIIAATALTLGSILASTPGVLANDVMVMDAFARASATS
                     MAKAGAVYMTLTNHGATPDRLLAASTPAAASAEVHESLEKDGVATMRPIVSLEIPAGG
                     SVQFKPGGYHIMLMGLKAPLKKGDMIMLQLKFEHAGLVDVMANVGDVAEGHSHTEGSS
                     GN"
     gene            complement(591..1115)
                     /locus_tag="PKV23_04650"
     CDS             complement(591..1115)
                     /locus_tag="PKV23_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012335324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004671 - protein C-terminal
                     S-isoprenylcysteine carboxyl O-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006481 - C-terminal protein methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HQX84758.1"
                     /translation="MWTNFTILALVTLQRLVELYIARRNTRRLLDNGGIEVSSGHYPL
                     IVLFHAIWLGGLWYLVWSETVSWPWAIAYLVLQAARGWIIAALGSRWTTRIIMVPGET
                     LSVAGPYKYFRHPNYMIVAAEIFILPLAFSMWWYAIAFGVLNLALLYWRIREEERALK
                     PLRGEVQFPDEPSV"
     gene            complement(1159..2052)
                     /locus_tag="PKV23_04655"
     CDS             complement(1159..2052)
                     /locus_tag="PKV23_04655"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HQX84759.1"
                     /translation="MPSQTPAQPLRAGLYMVIAMACFILNDSCIKIIGTTLPLGQIIT
                     IRGVLSVLVVALICAHQGVLGSLPTLFTPKVLARSLLDVAGTFLFLTALMHMPLANLT
                     AILQSVPLAVVLVSVIFLKEKVGMRRTVAIVTGFLGVLLIVRPTPSTFTIYELLALGI
                     VFILAFRDLITKRIPAHIPTFIIALANASFVALGGIIFGFYQGFLAVAPWQFGLLAAA
                     AVLLATGYVFMVATLRLGDLSGTAPFRYSNVLFAIILGMVFFGEFPDTLSYAGMGLII
                     AAGLYAAHREAVLGKAVKQPL"
     gene            complement(2067..3014)
                     /locus_tag="PKV23_04660"
     CDS             complement(2067..3014)
                     /locus_tag="PKV23_04660"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HQX84760.1"
                     /translation="MAGGWNFFPLHTPRMSSPQNHTLRAGLYMVITTACFVAGDTCIK
                     LIGATLPLGQVIGLIGVVSTFFLLVVCWQQGILKNLPMIYTRMVLLRSLFDAMGSFMF
                     VSALMHMPLANLSSVMQSVPLVVVVVGVIFLGERAGLSRIAAVIAGFVGVLLIVKPSP
                     QTITIYEFLAVGAVIVVAFRDLVTKRIPAHVPLLVIALANAIFISLSGFGVGLVQGFT
                     NIEAWQGALLMGAGLFVTISYFFIVATVRLGELSATAPFRYTEVVFAIIAGILVFGEY
                     PDAVAYMGMGLVVAAGLFAAHREAAQNRSAEAELMPPAF"
BASE COUNT          776 a          920 c          957 g          539 t
ORIGIN      
        1 tcaatgacta cgggtgtttc ctgcgccaat gcaggcgttg ccagcgccag cagggaaaat
       61 aaaccgcgcg acccgatgcc gcgcgtaaaa tgacacattc cagcctgaat ctgaggcaat
      121 ctccgcccca tgcgcaaact tgtcctcgaa atcattgccg ccacggcgct caccctcggc
      181 tccatcctag catcaacccc tggcgtattg gccaatgacg tgatggtgat ggatgccttt
      241 gcccgtgcct ccgccacctc catggcaaaa gccggcgcgg tctacatgac actcaccaat
      301 cacggtgcaa cgccggaccg tctgctggcc gccagcacgc cagccgcggc aagcgccgag
      361 gtgcatgaaa gtctggaaaa agatggcgtg gctaccatgc ggcccatagt gagccttgag
      421 attcctgcgg gcgggagcgt gcagttcaag cccggcggct accatatcat gctcatggga
      481 ttgaaagccc ccctgaaaaa gggcgatatg atcatgctcc agctaaaatt cgagcatgcc
      541 gggctggttg atgtcatggc caatgtcggc gatgttgcgg agggccactc tcacaccgaa
      601 ggctcatccg ggaactgaac ttcgccgcgc aggggcttga gcgcccgttc ttcctccctg
      661 atccgccagt agagcagggc caggttgagg acgccgaagg cgatggcata ccaccacatg
      721 ctgaaggcca gcggcaggat gaagatttcc gcagccacga tcatgtaatt ggggtggcgg
      781 aaatatttgt agggaccggc aacactcaag gtctcgccag gaaccattat gatgcgcgtg
      841 gtccagcggc tgcccagtgc ggcgataatc cagccgcgcg ccgcctgcag caccagatag
      901 gcaatggccc aaggccagct gaccgtttcg ctccacacca gataccaaag gccgccaagc
      961 cagattgcgt ggaacaggac aatcaaggga taatgaccgg agctgacttc aatcccgcca
     1021 ttgtcgagca ggcgcctggt gttgcgtcgc gcgatgtaaa gctcgaccag gcgttgcagg
     1081 gtgaccagcg caaggatggt gaagttggtc cacatctgtt tattgtagca tggtgaggaa
     1141 ccccgctgca aagtcccgtt acagcggttg tttcacggct ttgccaagca cggcttcccg
     1201 gtgtgcggca tagaggcccg cggcaatgat gagccccatg cccgcatagg acagcgtatc
     1261 gggaaattcg ccgaaaaata ccatgccaag gatgatggcg aacagcacgt tggaataacg
     1321 gaaaggcgcg gtgcctgaaa gatcgccgag gcgcagcgtg gccaccatga acacatagcc
     1381 cgtagccagc aggactgccg ccgccgcaag caggccgaat tgccatggcg ccaccgcgag
     1441 aaagccctgg tagaagccaa agatgatgcc gccgagggca acgaatgagg cgttggccag
     1501 cgcgatgatg aaagtcggga tatgggcggg tattcgtttg gtgatcaggt cccggaaggc
     1561 aaggatgaag acgatgccca gtgccaggag ttcgtagatg gtgaaggtcg aaggtgttgg
     1621 ccgcacaatg agcagtacgc ccaggaaccc tgtaacgatg gccacagtgc gccgcattcc
     1681 cactttctcc ttgagaaaaa taaccgacac gagaaccact gccagcggca cgctctgcag
     1741 aatggcggtc agattggcaa ggggcatgtg catcagtgcc gtgaggaaca gaaaggttcc
     1801 ggccacatcg agcagcgagc gcgccaggac cttgggggtg aacagggtgg gcaggctgcc
     1861 aagcacgccc tgatgggcgc agatcaaggc gaccaccagc accgacagaa ccccgcgaat
     1921 ggtgatgatt tgtcccagcg gcagcgtcgt gccgatgatt ttgatgcagc tgtcattgag
     1981 gatgaagcag gccatggcga tgaccatgta aagcccggcg cgaagcggtt gagcaggcgt
     2041 ttgggaaggc attgggcttg tatccctcaa aacgccggcg gcatcaactc agcctcggca
     2101 gaacggtttt gcgccgcttc ccggtgagcc gcgaaaagcc cggcggcaac gaccagcccc
     2161 atgcccatat aggcaaccgc atccgggtat tctccaaaaa ccaggattcc agcgatgatg
     2221 gcgaacacca cttccgtgta gcggaatggc gctgtggctg aaagctcgcc caggcgcacc
     2281 gtcgccacga taaagaagta actgattgtc acaaacagac ccgcccccat aagcagggcc
     2341 ccttgccagg cttcaatatt cgtgaagcct tgcaccagcc caacgccgaa gccgctcaac
     2401 gaaatgaaaa tcgcgttcgc cagcgctatc acgaggaggg ggacatgcgc cggtatgcgt
     2461 ttggtgacga gatcgcggaa agccacgacg ataaccgcgc cgactgccag aaactcatag
     2521 atggtaatgg tttgcggcga gggcttcaca atgaggagaa cccccacaaa ccccgctatc
     2581 accgccgcaa tgcgcgagag cccggccctt tcgccaagga aaatgacgcc aaccacgaca
     2641 acaaccagcg ggacgctttg catgaccgaa gaaagattgg ccagcggcat gtgcatgagc
     2701 gccgagacaa acatgaatga tcccatcgca tcgaagaggg aacgcagcag gaccatgcgc
     2761 gtgtaaatca tcggcagatt tttcagaatt ccctgttgcc agcaaaccac cagaagaaag
     2821 aaagtcgata caacaccgat caggccgatg acctgcccaa ggggcagggt ggcaccgatc
     2881 agcttgatgc aggtgtctcc ggccacaaaa caggctgtgg tgatgaccat gtagaggccg
     2941 gcgcgcagcg tatgattttg cggagaagac atgcgtgggg tgtgtagcgg gaaaaaattc
     3001 caaccgccag ccatgagcca tgcaatatcc gtcatgctga ggtgcgaccc ccggacttga
     3061 tcagggggag actcgaagca cctgctgcgc agagcacctc agggtgagga attacctacc
     3121 cccccgtcac actcatgtgc cgtgatacgg cgggcttgtt atggcgccgg tcgatgatga
     3181 agtcatggcc ct
//
LOCUS       DAORVN010000179         3191 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_26306_length_3191_cov_2.636480, whole genome shotgun sequence.
ACCESSION   DAORVN010000179 DAORVN010000000
VERSION     DAORVN010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3191)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3191)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3191
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26306_length_3191_cov_2.636480"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            75..1313
                     /locus_tag="PKV23_04665"
     CDS             75..1313
                     /locus_tag="PKV23_04665"
                     /inference="COORDINATES: protein motif:HMM:NF014769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX84761.1"
                     /translation="MDKRTSLIPGYEIILDKLVDIALAAGPIGSQTDKYFTNTSRIVA
                     AHGDTDVTFAVFMRRRVVAALEPTIRMVQRLVPTARVKRFYEEGDIVPSESKMLEITG
                     SMAKLSEVETLVLQKIGFPCVSANNAYEMCRAIPYAAFMDMHARHGSGAEMNILAAYG
                     AAVGSAAAKHADSNVKGFVGSSQDLTAPLFGSASGMGTMPHALVGYTRGDVLEAMKLF
                     AETIPEAKTLIALVDYTGEEVTDSLRSARWFYEEAKLQDTDKVFGVRLDTHGGRFAEG
                     LDYEKSVDTVGHWLGVTGEYNIVEQVLGGRAFQLDPNNILVDKVRRILFGKGVSVASI
                     IHVRRALDAAGYKQAQIVASSGFDPQKCQVMGAAKAPLNMVGTGSFLPATLTETYATA
                     DIIAYDGVRRVKVGREFLFD"
     gene            complement(1310..1534)
                     /locus_tag="PKV23_04670"
     CDS             complement(1310..1534)
                     /locus_tag="PKV23_04670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX84762.1"
                     /translation="MDCDEISMTTFTAGALAALLAADGKAKSSVAASAKLMERRNRIT
                     IVLSPERVPRENKHQIFTRRKMAAIHMPVS"
     gene            1533..1853
                     /locus_tag="PKV23_04675"
     CDS             1533..1853
                     /locus_tag="PKV23_04675"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013895020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HQX84763.1"
                     /translation="MSVKVNGWHYGKWKVSTARPGYYTPRGVFRVQRTARVYYSRKYD
                     NSPMPNSVFFKGGYAIHGSYYVKSLGRPASHGCVRLHPSHAAKLYNLVQKYGPKSTRI
                     TVVN"
     gene            2031..>3191
                     /locus_tag="PKV23_04680"
     CDS             2031..>3191
                     /locus_tag="PKV23_04680"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HQX84764.1"
                     /translation="MKFGYFANVTNRHDQPFGVLMNEQIALAQYLDTHDWDSVWYTEH
                     HFSYEGFEVVPNPVLMSTWTAAHTKRLRIGQAANIITFWHPLRFAEDIAMLDHMSGGR
                     IEVGVGRGIYGREALQLNKMADTRNPAQNFRIFAEALEVVRRAWSNQYFEFHGEFFNF
                     PDRGFIWDHGMSPKSADYQNMETFELEKLTVVPRTLQQPTPPIHQVVDGHLAIQYAAE
                     NNMRAMMWIPPTDALKPRFELYRDKKAAKEQRAVNLGEGVSLVRDMFCAESKAEAKRI
                     GGAGIVDYLRWVCHWRGLDNHRHLGEELPKTENKLDLLSVDFLDERNLLFGTPDEISA
                     RIEEMIDVLNLQHLLVWSHFPGIEHEAAMRSVKLFTDEVMPRFKGRVGLHQAA"
BASE COUNT          690 a          856 c          941 g          704 t
ORIGIN      
        1 aggcgccgaa agcctcataa aggcggtaaa gggcgtttga cgtccgccgc ctttcttggg
       61 acaatgagat gatgatggac aagcgcacct cgcttattcc cgggtacgaa attatcctcg
      121 acaagctcgt agatattgcg ctggcggccg ggcccatcgg cagccagacg gacaagtatt
      181 tcaccaatac ctcgcgcatc gttgcggccc atggcgacac ggatgtgacc tttgccgtgt
      241 tcatgcgcag gcgcgtggtg gcggcgcttg aacccactat ccgcatggtg cagcggctgg
      301 tgccaacggc gcgggtcaag cggttctatg aagagggcga catcgttccc tcggaaagca
      361 agatgctgga aatcaccggc tccatggcca agctttcgga agttgaaacg ctggtgctgc
      421 agaagatcgg ctttccctgc gtatcggcca acaatgccta tgaaatgtgc cgggccatac
      481 cttatgccgc cttcatggac atgcatgcgc gccacggcag cggggcggag atgaatattc
      541 tggccgctta tggcgcggcg gtaggcagtg cggcggccaa gcatgcagac agcaacgtga
      601 agggttttgt tggctcatcc caggatttga cggctcccct gtttggttct gcttccggta
      661 tgggcaccat gccgcacgct ctcgtgggat atacgcgggg cgatgtactg gaggccatga
      721 agctttttgc cgagaccatt cccgaggcca agacgctgat cgcgctggtt gactatacgg
      781 gtgaggaagt gaccgactcc ctgcgcagcg cgcggtggtt ctacgaagag gcgaaactgc
      841 aggatacgga caaggttttt ggagtgcgcc ttgacaccca tggcgggcgc tttgccgaag
      901 ggctggatta tgaaaagtcc gtcgacacag tgggccattg gctgggcgtt acgggcgaat
      961 acaacatcgt cgaacaggtt ctgggaggca gggccttcca gcttgatccc aacaacattc
     1021 tggtggacaa ggtgcgccgc attctgttcg gcaaaggtgt gtcggttgca tccatcatac
     1081 atgtgcgcag ggccctggac gcagccggat acaagcaggc gcaaatcgtt gcctcctcag
     1141 ggtttgatcc gcagaaatgc caggtgatgg gggcggcaaa agccccgctc aacatggtcg
     1201 gcaccggcag cttcctgcct gcgacactca ccgaaaccta tgctaccgcc gatatcattg
     1261 cctatgacgg ggtgcgccgt gtgaaagtgg gccgcgagtt tcttttcgac tagctcacag
     1321 gcatgtgaat tgccgccatc ttgcgcctgg tgaaaatctg gtgtttgttt tctcggggca
     1381 cacgttcagg ggacagcact atggtgattc gattcctccg ttccataagc tttgcacttg
     1441 ccgcaacact gctttttgct tttccgtctg cggccaagag cgcagctaac gcgccggcgg
     1501 taaatgttgt cattgatatt tcatcgcaat ccatgtcagt caaggtaaac ggctggcact
     1561 acgggaaatg gaaggtttcc actgcccggc caggctatta cacgccgcgg ggcgttttcc
     1621 gggtgcaacg gacagccagg gtttactact caagaaagta tgacaattca cccatgccga
     1681 actccgtgtt tttcaagggc ggatatgcca tccatggcag ctattacgtg aagtccctgg
     1741 ggcggccggc atcgcatggc tgtgtgcggc tgcatccctc ccatgccgcc aaactttata
     1801 atctggttca gaagtatggc ccgaagtcga cccggatcac ggtcgtcaat taagggcatt
     1861 gaaagcgggc gtacgcgccc taaccgtcta aatcttcgct tcccaaatgc tctgggcagt
     1921 gacaatctgg ctttgacctg atagggaaat ggcagctgaa gtcggcaggc agtcagcgaa
     1981 accggtgtgt gccatgaact ctgaacccct atcagaagga gccacacaga atgaaattcg
     2041 gatattttgc caacgtaacc aaccgccatg accagccttt tggcgtgttg atgaatgagc
     2101 aaattgccct tgctcagtac ttggacaccc atgactggga ttccgtatgg tacacggaac
     2161 atcacttttc ctatgagggc tttgaagttg ttcccaatcc ggttctgatg tccacctgga
     2221 ccgcggccca caccaagcgc cttcgtattg ggcaggcggc caacatcata acattctggc
     2281 atcccctgcg ttttgccgag gacattgcga tgcttgacca catgtcaggc ggacgcatcg
     2341 aagtgggcgt tggccgcggc atttacggcc gcgaagccct gcagctcaac aagatggctg
     2401 atacccgcaa tccggcacag aatttccgga tttttgccga ggctttggaa gttgtccgaa
     2461 gggcctggtc aaaccagtac ttcgaatttc acggggaatt tttcaatttt cctgatcgcg
     2521 gtttcatctg ggatcacggc atgtcgccga agagcgctga ttatcagaat atggaaacct
     2581 ttgagctgga gaagctcaca gttgttcccc gcaccctgca gcagccaacg ccgcccattc
     2641 atcaggtggt ggatggccat ttggccattc aatacgcggc ggaaaacaac atgagggcca
     2701 tgatgtggat cccgcccaca gatgcgctga aaccccggtt cgagctgtac cgtgacaaga
     2761 aggcggcaaa ggaacagcgc gccgtaaacc tcggtgaggg ggtcagcctg gtgcgcgaca
     2821 tgttctgtgc cgaaagcaag gcggaagcaa agcggattgg cggggcggga atcgtggatt
     2881 acctgcgttg ggtttgccat tggcgcgggc ttgataacca ccggcatctt ggcgaggaat
     2941 tgccgaaaac ggagaacaag cttgacctgc tctccgttga ttttcttgac gagcgcaact
     3001 tgctgttcgg gacaccggat gaaatatccg cccggattga ggaaatgatc gatgtcctga
     3061 atctgcagca cctgctggtt tggtcgcatt ttccaggaat tgagcatgag gcagcgatgc
     3121 gttcggtgaa gctcttcacc gatgaagtga tgccacgatt caagggccgg gtcggcctgc
     3181 accaggcggc g
//
LOCUS       DAORVN010000180         3186 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_26373_length_3186_cov_4.238263, whole genome shotgun sequence.
ACCESSION   DAORVN010000180 DAORVN010000000
VERSION     DAORVN010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3186
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26373_length_3186_cov_4.238263"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..913
                     /locus_tag="PKV23_04685"
     CDS             <1..913
                     /locus_tag="PKV23_04685"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018095681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HQX84765.1"
                     /translation="PNLVQTLENNPAFVHGGPFANIAHGCNSVMATKAGLKLADYVVT
                     EAGFGADLGAEKFFDIKCRKAGLTPAATVIVATIRALKMHGGVAKEELKKENTEALKK
                     GMVNLGRHIENMKKFGVPIAVAINHFVTDTDADIEVIRSFCKEHGVEAFKCTHWAEGG
                     KGTEALAKHVAELADRSSVQFKPIYGDDMPLWDKIKTIAREIYRADDIVADKAVRDQL
                     KSYESQGYGKFPICMAKTQYSFSTDPNLKGAPINHIVPIREVRLSAGAEFIVVVCGDI
                     MTMPGLPKVPSAEVIGLDAKGQIAGLF"
     gene            979..2127
                     /locus_tag="PKV23_04690"
     CDS             979..2127
                     /locus_tag="PKV23_04690"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HQX84766.1"
                     /translation="MVEMRKLLISLAALLLVAAGYAYSQLGPFNGPPRAPPRDRWEAL
                     LRERIKLPEGYSFEVFARDLGRLRLMQMTENGDLIVSGYRDGNILLLKADRDGDGRSD
                     GQDELIEGSNQPHGLLLEGSTLYVAEEQRVVRYDFDGAQLANERVILEGIPGGGGHSS
                     RTLKRGPDGFLYVSIGSSCNACIEDHPWRAAILRFREGATPEIFASGLRNTVGFDWQP
                     ETGALFGVDNGRDHMGDDVPDDEVNRIAAGGHYGWPHVHGAEVRDPSLYAEMPAGLSP
                     VPQFHGLGGHVAPLSIRFLRNQANARLNGTALVAEHGSWNRREKAGYRIIRLILEGST
                     AREEVFLGGCEVNGEVICRPVDILEAPDGRLYVSDDYAGAVYTITKSP"
     gene            2327..2476
                     /locus_tag="PKV23_04695"
     CDS             2327..2476
                     /locus_tag="PKV23_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008194643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HQX84767.1"
                     /translation="MFDFVKTRYTRWQRYSRTKSELESLSNRELADLGIMRTDIHRLA
                     REATR"
     gene            2499..2660
                     /locus_tag="PKV23_04700"
     CDS             2499..2660
                     /locus_tag="PKV23_04700"
                     /inference="COORDINATES: protein motif:HMM:NF018294.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HQX84768.1"
                     /translation="MPLFKFLSRRNRLKAYRQARREVEMISDADLADMGIKRYQLEAA
                     ANAKVMGLA"
     gene            2915..>3186
                     /locus_tag="PKV23_04705"
     CDS             2915..>3186
                     /locus_tag="PKV23_04705"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015933678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HQX84769.1"
                     /translation="MAATDQAISGVPGRYAAALFELAAEAKAIDKISDGLSTFQTMLA
                     GSADLQRLVRSPVFSGEDQMAALEAVCAKAGVNGIALNFIKLMAKNR"
BASE COUNT          684 a          869 c         1000 g          633 t
ORIGIN      
        1 gccgaacctg gtgcagacgc tggaaaacaa tccggccttc gtgcatggcg gcccgtttgc
       61 caacatcgcc catggctgca attcggtgat ggcgaccaag gcggggctga agcttgccga
      121 ttatgtggtg actgaagcgg gcttcggggc cgatctcgga gccgagaaat ttttcgacat
      181 caagtgccgc aaggcggggc tgacacctgc cgcaactgta attgttgcaa ccatccgggc
      241 gctcaagatg catggcgggg tggccaagga agagctgaag aaggaaaaca ccgaagccct
      301 caagaagggc atggtcaatc tcggccggca catcgagaac atgaaaaaat tcggcgtgcc
      361 gattgcggtg gcaatcaatc atttcgtgac cgatacggac gcggacatcg aggtcatccg
      421 cagcttctgc aaggagcatg gggttgaggc cttcaagtgc acgcactggg ctgagggcgg
      481 caaaggcacg gaggccttgg ccaagcatgt ggcggagctt gccgaccgga gctcggtgca
      541 gttcaagccg atctatggcg atgacatgcc gctgtgggac aagatcaaga ccatcgcgcg
      601 cgaaatctac cgggccgatg acatcgtggc cgacaaagcg gtgcgcgacc agctcaagtc
      661 ctacgagtca cagggctacg gcaaattccc gatctgcatg gccaagacgc agtattcctt
      721 ctcgaccgat cccaatttga agggcgcccc catcaaccac atcgttccca tccgcgaagt
      781 gcggctctcg gcgggggcag agttcatcgt ggtggtatgc ggcgacatca tgaccatgcc
      841 gggcctgccc aaggtgccat cggcggaagt gatcgggctt gatgcaaagg ggcaaatcgc
      901 cgggttgttc taggacctca ccaaagcgtg atgggaagct ggtgcaaagt cctttcgcag
      961 gttttccgat gcgcttaaat ggtggagatg cgcaagctcc tgatttccct tgcggccctg
     1021 ctgctcgttg cggcgggtta tgcctactcc cagctcggcc cattcaatgg acccccgcgg
     1081 gcgccaccgc gggaccgatg ggaagcattg ctccgtgaac ggataaaatt gccggaaggc
     1141 tacagcttcg aggtttttgc ccgtgacctg gggcggctgc gcctgatgca gatgacggaa
     1201 aatggcgatc tcatcgtgtc gggctaccgc gacggaaaca tcctgctgct gaaggcagac
     1261 cgtgatggtg acgggcgcag cgacggacag gatgaattga tcgaaggctc caatcagccg
     1321 catggcctgc tgcttgaggg ctccacgctt tatgtggcgg aagagcagcg ggtggtgcgc
     1381 tatgatttcg atggcgcgca gcttgcaaac gaacgggtga ttctcgaagg cattcccgga
     1441 ggaggcgggc actcgtcacg caccctcaaa cgcgggccgg acggattcct ctatgtgtcg
     1501 attggctcaa gctgcaatgc ctgcatcgag gaccatccct ggcgggcggc gatcctgcgg
     1561 ttcagggaag gcgccactcc ggaaatattt gcctcggggc tccgcaatac ggtgggtttt
     1621 gactggcagc cggagacggg ggcgctcttc ggcgtggata atggccgcga ccatatgggg
     1681 gacgatgttc ccgacgacga ggtgaaccgc atcgcggcgg gaggacatta tggctggccc
     1741 catgtgcatg gtgccgaggt gcgggaccca agcctttatg cagaaatgcc ggcgggactt
     1801 tccccggttc cgcagtttca tgggcttggc gggcatgtgg cgccgctgtc gatccggttc
     1861 ctgcggaatc aggccaatgc gaggctgaac ggcacggctc tggtggcgga acacgggtcg
     1921 tggaaccgcc gggaaaaggc gggataccga atcatccggc tgattcttga aggctcaacg
     1981 gcccgcgaag aggtgtttct ggggggttgc gaggttaacg gggaggttat ctgccgcccg
     2041 gtggacattc tggaggcccc ggatggccgc ctgtatgtga gcgatgatta cgcaggggct
     2101 gtctacacaa tcaccaaatc gccctgaact gcccctgaat ggggcagcag tgcgcgaatt
     2161 tatggcatcg cagcattaaa aatcatcatt atgaacgaat tgtaaacaat tgatatattt
     2221 gacgtaattg ctgtcatgca aaatttgcaa tactcatttt gctgcatcgc acctaaatac
     2281 tgactgttaa agcaacccac tacccgtcaa tccaagggga atgaccatgt tcgatttcgt
     2341 aaagacccgt tacacacgct ggcagcgcta cagccggacc aagtccgagc tcgaaagcct
     2401 ttcaaaccgc gagctcgcgg atttgggcat catgcgcacc gacattcatc gtctggcgcg
     2461 cgaagcgaca cgataaacca attccgatgg aggcattgat gccactgttc aaattcctga
     2521 gccgcagaaa ccgcctgaag gcctatcgcc aggcccgccg ggaagtcgag atgatttccg
     2581 atgcggacct cgccgacatg ggcatcaagc gctaccagct tgaagccgcg gcaaatgcaa
     2641 aagtcatggg cctggcctag agtcagtccc gtttcagccg ggcctgccat ctcctgaccg
     2701 gggtggcagg ctttttcttt gcacaaagtt tgatattgcg gcaatccgcg cgattaaggc
     2761 ggttgccagt ctttatctcc tgtgttaccg agacgccgcg gcagggcggg gttctgttcc
     2821 gtgcctttgc cttgtgcagg gggttgatca aaaaaatcaa gtgtttgggt tgcgttccgt
     2881 ggccacgaca cgagactgtt tcgaaagaga aatcgtggca gccacagatc aggcaatttc
     2941 cggcgtgccg ggccgatacg cagcagcgct ctttgagctg gcggccgagg caaaagccat
     3001 cgacaagatc agcgatgggc tttcaacatt tcaaaccatg ctggcgggat cagctgactt
     3061 gcagcgcctg gtgcgctcgc cggtattttc cggcgaagac cagatggcgg ccctcgaggc
     3121 ggtgtgcgcc aaggccgggg tcaacggcat cgcgctcaat ttcatcaagc tgatggcgaa
     3181 gaaccg
//
LOCUS       DAORVN010000181         3179 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_26485_length_3179_cov_2.600192, whole genome shotgun sequence.
ACCESSION   DAORVN010000181 DAORVN010000000
VERSION     DAORVN010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3179
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26485_length_3179_cov_2.600192"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..768
                     /locus_tag="PKV23_04710"
     CDS             <1..768
                     /locus_tag="PKV23_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQX84770.1"
                     /translation="KVSAGEMRHPPRITMGYFAQHQLDELSEDLSPYEYISTLLPDAT
                     VAQRRARLGAVGFGAALADSKCSVLSGGEKARLVFMLATFHGPHVLILDEPTNHLDVD
                     SREALIMAINDYEGAVILISHDRHIIETCADQLWLVRDGTVKPFDGDMDEYTDIVLGR
                     ATAVKKVEKAAAPAAKPLKKLMTPVIKKKIQELDQKMLKIKDKIILLEQALADHTIYT
                     QEPKKAADFARLKSKFESELEREETHWLELHESTLNA"
     gene            761..2059
                     /gene="nhaD"
                     /locus_tag="PKV23_04715"
     CDS             761..2059
                     /gene="nhaD"
                     /locus_tag="PKV23_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter NhaD"
                     /protein_id="HQX84771.1"
                     /translation="MLNLTETWYGYLALGIFITAYILVILEETTQLRKSKPVLLAAGL
                     IWALIGMAYAAAGAGDKAEEAAQHVIMEYGELFLFLLVAITYVNTLEERRVFDVLRVK
                     LTGKKLSYRNLFWMTGTLAFFLSGVLDNLTTSLVMGAVVLAIGRDSPKFVAIGFINIV
                     VAANAGGAFSPFGDITTLMVWQKDKVTFLEFFALFVPSLVNWLVPAIIMHFGIPQKTP
                     VAHTETVRPKPGAMGVIVFFALTIATAVSFRNFLHLPPAMGMMLGLGYLQAWSYFLQR
                     KGSRLGNDDMVLNSFKQIERVEWDTLLFFFGILFAVGGLGVMGYLAMASEYFYVGLGP
                     TVANIAVGVMSAVVDNIPVMFAVLTMDPAMSHGQWLLVTLTAGVGGSLLSIGSAAGVA
                     LMGQAPGQYTFFSHLKWSWAIALGYAASIATHLFINASLF"
     gene            2061..>3179
                     /gene="nhaA"
                     /locus_tag="PKV23_04720"
     CDS             2061..>3179
                     /gene="nhaA"
                     /locus_tag="PKV23_04720"
                     /inference="COORDINATES: protein motif:HMM:NF007111.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HQX84772.1"
                     /translation="MNDQSSLSRFINHEATAGFALVAAAAAALVLTNIGFAESYDYFL
                     ELPVSVQIGPIGLDKTLVHWVNDGLMAIFFFLVGLEIKREVLEGNLSSREQIILPAVA
                     ALGGMLAPALVYALINWGNAETLQGWAIPAATDIAFALGALALVGSRVPLSLKVFLLT
                     LATLDDVGAIIIIAIFYASDVSLVGLGLAGVAFAGLLVLNRLRVDRTAPYLFLGLAMW
                     VFVLESGVHATLSGVALALTIPLLRSDGSSFIHALEEGLHPYVKFLILPLFAFANAGI
                     PLAGMSMDSLTSSLPLGIAAGLVLGKPLGILLAVFACVSLGFAKLPEEATLTHVAGVG
                     CLAGIGFTMSLFIGTLAFDTAAQIDAVRVGVIAGSVIAT"
BASE COUNT          575 a          943 c          932 g          729 t
ORIGIN      
        1 aaggtgtcgg ccggcgaaat gcgccatccg ccgcgcatca ccatgggcta cttcgcccag
       61 caccagctgg atgaattgtc ggaagacctt tcgccttacg agtatatttc caccctgctg
      121 cccgatgcca ccgtggcgca gcgccgggcc aggctcggcg cggtgggttt tggcgcggcc
      181 cttgccgatt ccaaatgttc ggtgctgtcg ggcggcgaga aggcgcgcct ggtgttcatg
      241 ctggccacct tccatggccc ccatgttctc attctcgacg agcccaccaa ccatcttgac
      301 gttgacagcc gcgaggccct gatcatggcg atcaatgact acgagggcgc cgtcatcctc
      361 atcagccatg accgccacat catcgagacc tgcgcggacc agttgtggct ggtccgggac
      421 ggcacggtga agcctttcga tggcgacatg gatgaataca ccgatatcgt gctgggccgt
      481 gccactgccg tgaagaaagt ggaaaaggcg gcagcacccg ccgcaaagcc gctgaaaaaa
      541 ctgatgacgc cggtcatcaa gaagaagatc caggaacttg atcagaaaat gctgaaaatc
      601 aaggacaaga tcatcctgct ggaacaggcg ctggcggacc acacgattta tacacaggag
      661 cccaagaagg ccgccgattt tgcgcgactc aagagcaaat tcgagtcaga actggaacgc
      721 gaggaaacgc actggcttga actgcacgaa agtacgctga atgcttaatc ttacggaaac
      781 ctggtatggc tatctggcgc ttgggatttt cataaccgcc tacatcctgg tgattctcga
      841 agaaaccacg cagcttcgca agtccaagcc ggttctcctt gcggcgggcc tcatctgggc
      901 attgatcggc atggcctatg cggccgccgg cgcgggggac aaagcggaag aagccgcgca
      961 gcatgtgatc atggaatatg gcgaactctt cctcttcctg ctggttgcca tcacctatgt
     1021 gaacaccctg gaagagcggc gggtgttcga cgtcctgaga gtgaagctta ccgggaaaaa
     1081 gctgtcttac cgcaacctgt tctggatgac cggcaccctg gcatttttcc tctctggggt
     1141 gctcgacaac ctgacgacgt ccctggtgat gggcgcggtg gttctggcaa tcggcaggga
     1201 ttcgccaaaa ttcgttgcca tcggcttcat caacattgtc gtggcggcaa acgcgggtgg
     1261 ggcattctcc cccttcggcg acatcacgac cctgatggtc tggcagaagg acaaggtcac
     1321 gttcctggaa ttcttcgcac tcttcgtgcc atcgctggtg aactggctgg tgcctgccat
     1381 catcatgcat ttcggaattc cgcagaagac accggtggcc cacacggaaa ctgtgcgccc
     1441 caagccgggc gccatgggcg tgattgtgtt ttttgcgttg acgatagcga ccgccgtttc
     1501 tttccggaac ttcctgcatc ttccgccagc catggggatg atgctgggcc tgggttacct
     1561 gcaggcatgg tcctatttcc tgcagcgcaa gggctcgcgc ctggggaacg acgacatggt
     1621 cctgaactcc ttcaagcaaa tcgagcgggt agaatgggac acgctgctgt tcttctttgg
     1681 cattctattt gccgtgggtg ggcttggcgt tatgggctac ctcgccatgg ccagcgagta
     1741 tttctatgtc gggctggggc caacggtcgc caatatcgcc gtcggcgtga tgtcggctgt
     1801 tgtcgacaac attcccgtca tgtttgccgt gctcaccatg gatccggcca tgagccatgg
     1861 ccagtggctt ctcgtcacgc tcacggcggg agtcggcggc agcctgctgt ccataggctc
     1921 cgcggccggt gttgccctca tggggcaagc tccggggcag tacactttct tcagccatct
     1981 gaaatggagc tgggcgattg cgctgggata tgcggcgagc atcgccacgc atcttttcat
     2041 caacgcatcg ctgttctgat atgaacgacc agtcatccct ttcgcgcttt atcaaccatg
     2101 aggccacggc gggtttcgcc ctggtggctg ccgctgccgc cgcgctcgtt ctgacaaaca
     2161 tcggctttgc ggaatcctat gactacttcc ttgagctgcc agtctcggtc caaatcggac
     2221 ccatcggact ggacaagacc ctggtccact gggtcaatga cgggctcatg gccattttct
     2281 tctttctcgt ggggctggaa atcaagcgtg aagttctgga aggaaacctc tcatcgcgcg
     2341 aacagatcat cctgcccgcc gttgcagcac ttggcggcat gctggcgcct gccttggttt
     2401 atgctctcat caactggggc aatgctgaaa ccctgcaagg ctgggccatt ccggcggcaa
     2461 ccgatatcgc ttttgcgctt ggggcattgg ccctcgtcgg gtcccgcgtg ccgctgtcgt
     2521 tgaaagtctt cctgctgacg cttgcgaccc tggacgacgt tggcgcaatc atcatcatcg
     2581 caatttttta tgcctcagac gtgtcgcttg tcggcctggg cctggcaggc gttgcatttg
     2641 ccgggctgct tgttctcaac cgtctgcggg tggaccgaac cgcgccctat ctgtttctcg
     2701 gccttgcgat gtgggtcttt gtcctggagt cgggggtcca cgccaccctg tcgggggttg
     2761 ccctggctct caccattcca ctgctgcgca gcgatggatc gagcttcatc cacgccctgg
     2821 aagaaggcct gcacccttat gtcaaatttc tcatcctgcc gctgtttgcc ttcgccaatg
     2881 caggcatacc gctggccggc atgtcgatgg attcgctcac gtcatccctg ccgcttggca
     2941 ttgccgctgg cctggtgctt ggcaagcctt tgggcattct ccttgccgtg ttcgcgtgtg
     3001 tctcgctggg cttcgcaaaa ctgcctgagg aggcaacgtt gacgcacgtg gcgggggttg
     3061 gctgccttgc cggcattggc ttcaccatga gcctgttcat cgggactctc gcctttgaca
     3121 cggccgcaca gatcgacgcg gtgagggtcg gcgtcatcgc ggggtcagtt attgccacc
//
LOCUS       DAORVN010000182         3173 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_26578_length_3173_cov_2.819756, whole genome shotgun sequence.
ACCESSION   DAORVN010000182 DAORVN010000000
VERSION     DAORVN010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3173
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26578_length_3173_cov_2.819756"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(59..1282)
                     /locus_tag="PKV23_04725"
     CDS             complement(59..1282)
                     /locus_tag="PKV23_04725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HQX84773.1"
                     /translation="MRGLSGKVLLLTIIFVMLGEVLIFLPSIANFRIQWLKGRIAQAE
                     IAALAAEAAPDQILSSDLRSEILMGAGVLVVSLEKGESRKLALRSDTDFMIDASFDLR
                     STMWYEAVTDAFGAMWAGESRVISVMDMPPNMSGDLIEIALYEEPLRAAMFRYAINIL
                     LLSIFLSLLVAGMIFAALNWVLVKPMQRLTRNMVEFGRNPEDFSRIIKPGAGRDEIGI
                     AERELHDMQTELATMLQQKNHLAALGLAVSKVSHDLRNMLTSAQLISDRLSDVEDPTV
                     KRFAPKLISSLDRAIEFLSQTLKFGRAQELPPQRELLELRELADEVIETAVIKASSRI
                     RIPWRTMACWLRPSSPSGFPTARRSRPRKPCGRTRSFASDRRSKFAIRRKARHRLGLP
                     AISTRCNWPWAPWAS"
     gene            complement(1332..2729)
                     /locus_tag="PKV23_04730"
     CDS             complement(1332..2729)
                     /locus_tag="PKV23_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008593866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQX84774.1"
                     /translation="MIYDLAIIGAVSGGLSFAAAAAQFGQKVILFEKGKMGGDCLNYG
                     CVPSKALIAAAKHAHVFRTSGPYGIAAAEPKVDFKKVRDHIRKVIAAIEPHDSAARFE
                     KLGVKVVPGAAKFRDAKTLESGGGIFSAKRIVIATGSRPAVPAIPGLETVAYFTNETI
                     FENSVLPKHLIIIGGGAIGMELAQAHRRLGSAVTVIEAFTPLAKDDPELAAVVLESLR
                     RDGVKIFAETAIKKISKAKPGIAIETAGHGKITGSHLLVATGRAANVEGLNLEAAGVV
                     YTAKGVTVDKGLRTSQKHIYAIGDVAGGLQFTHVANYHAGLVIRNALFRLPVSNRNDI
                     IPHVTFSEPELAQVGFTEAQALKDYGPSIMVVRWPFLDNDRAQAEGKTTGLIKAVIGK
                     RGRILGVGIVGANAGEVIQPWVLAMNEGLKIRAIAGMVAPYPTLGEINRRVAVNYFSG
                     LASNPWLRRVIRLVS"
     gene            complement(2726..>3173)
                     /locus_tag="PKV23_04735"
     CDS             complement(2726..>3173)
                     /locus_tag="PKV23_04735"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HQX84775.1"
                     /translation="IIFLAARTPLEAALSRKAGGIVGKLQEGFARDAFSYLLFLRLTP
                     VFPFWLVNIAAALAQVRLKTFVSATLIGIIPAGFAFSFVGSGLDSLIDSQQLAYDACL
                     ARTPATPCSFDLSASRIITPQILLALAALGVAALIPVLAKRLRARP"
BASE COUNT          647 a          955 c          960 g          611 t
ORIGIN      
        1 gatccggcca aacgtcgggc gggaagtcac ccccgcctca tgaggccgat gagaagaatc
       61 acgaagccca gggcgcccac ggccagttgc atcgtgtaga gattgccggc aagccgagcc
      121 gatgtcgggc tttccggcgg atagcgaact tcgaccttct gtccgacgcg aagcttcgtg
      181 ttcgtccgca gggcttccgt ggcctcgatc gtcgcgccgt cgggaagcct gaaggcgatg
      241 acgggcgcaa ccaacacgcc atcgtccgcc atgggatgcg gatccggctc gaggccttga
      301 tcaccgccgt ttcaatcacc tcgtcggcca gctcgcgcaa ttccagcagc tcgcgctgcg
      361 gcggcagttc ctgggcccgg ccgaacttca gcgtctggct gaggaactcg atggcccggt
      421 ccagcgaact gatgagcttc ggcgcaaagc gcttgaccgt cgggtcctcc acatcggata
      481 gccggtcgga aatgagctgg gccgaagtga gcatgttgcg caagtcgtgg ctgaccttgc
      541 tcaccgccag gccaagggcg gcgagatggt tcttctgctg cagcatggtg gcaagctcgg
      601 tctgcatgtc gtgcaactcg cgctcggcga tgccgatttc atcacgcccg gccccgggct
      661 tgatgatgcg cgaaaaatcc tccggattcc ggccaaactc aaccatattg cgcgtgaggc
      721 gctgcatggg tttcaccagc acccagttca gcgccgcgaa aatcatgccc gcgaccagca
      781 gcgaaaggaa aatggacagc aggagaatgt tgatggcgta gcggaacatg gcggcccgca
      841 gcggttcttc gtaaagtgcg atttcaatca ggtcgccgga catgttgggc ggcatatcca
      901 tgacgctgat cacccgggat tcgcccgccc acatggcgcc gaatgcgtcg gtgacagcct
      961 cgtaccacat ggtgctgcgc agatcgaaac tggcatcgat catgaagtcc gtgtcgctgc
     1021 gcagcgccag cttgcggctt tcgccttttt cgagggagac gacaagcacc cccgcgccca
     1081 tcaggatctc gctgcgcagg tcgctggaca gaatctggtc gggggcggct tcggcggcaa
     1141 gggcggcgat ctcggcctgg gcgatgcgtc ccttgagcca ttggatgcgg aagtttgcaa
     1201 tggacggcag gaagatcaga acctcgccca gcatcacgaa gatgatggtc agcagcagga
     1261 ccttgccgga aaggccgcgc atggccccag gctgttttga tcggtttggc gcttgctcct
     1321 gcattcagcc tctagctcac cagtcttatt acgcgccgca gccacggatt ggatgcaagc
     1381 cctgagaaat aattaaccgc cacccggcgg ttgatctcgc ccagcgtcgg gtagggggcc
     1441 accataccgg caattgcgcg gattttgagg ccctcgttca tcgccagaac ccagggctgt
     1501 atgacttccc cggcgtttgc gccaacgatg cccaccccca gaatccggcc tctttttccg
     1561 ataacggcct tgatcaggcc ggtggtcttt ccctcggcct gggcccggtc attgtcaaga
     1621 aaaggccagc ggacaaccat gatggaggga ccatagtcct tgagggcttg cgcttccgta
     1681 aatcccacct gggccagttc cggctcgctg aaggtgacgt gcggaatgat gtcgttccgg
     1741 ttgctgacag gcaggcggaa cagggcgttc cggatgacga gccccgcgtg ataattggcg
     1801 acatgggtga actgcagtcc gcccgccaca tcgccgatgg catagatatg cttttgcgaa
     1861 gtccgcaggc ccttgtcgac ggtcacgccc ttggctgtat agacaacgcc ggccgcttcc
     1921 aggttcagtc cctcgacatt tgccgcccgc cccgtggcga caagcagatg actgccggtg
     1981 atctttccat gcccggctgt ttcgatagcg atgcctggtt ttgctttcga gattttcttg
     2041 atggccgttt ccgcgaaaat ttttaccccg tcgcggcgca ggctctccag aacaacggcc
     2101 gccagttccg gatcatcctt ggccagcgga gtgaaggcct cgatgaccgt aacggcggaa
     2161 cccaatcggc gatgagcctg tgccaattcc atgccgattg cgcccccgcc aatgatgata
     2221 agatgcttgg gcaatacgct gttctcaaaa atggtttcgt tggtgaaata ggcaactgtt
     2281 tcaagtccgg gtatcgccgg aacagcgggg cgggaacccg tggcaatcac aatgcgcttg
     2341 gctgagaata tgccgccgcc ggattcaagg gtcttcgcat cacggaattt tgcagcaccc
     2401 ggcacgacct tgacaccgag cttttcaaag cgtgcggcgg aatcatgggg ctcaatggcg
     2461 gcaatcactt tccggatatg gtcacgcacc ttcttgaaat ccaccttcgg ctcggcggct
     2521 gcgatgccat agggccccga agtgcgaaag acatgggcat gctttgccgc cgctatgagt
     2581 gcctttgacg gaacgcagcc atagttgagg caatcgccgc ccatcttgcc cttttcgaag
     2641 aggatgacct tttgcccgaa ctgagccgcc gcggccgcaa acgataaccc gccggaaacg
     2701 gcgccgataa tcgccaggtc atagatcatg gtctggcccg caaacgcttc gccagcaccg
     2761 ggatcaacgc cgcaacgccc agagcggcaa gcgccaggag aatttgcggc gtgatgatcc
     2821 gcgaagctga aagatcaaag ctgcagggtg ttgcaggagt ccgcgccagg caggcatcat
     2881 aggcgagctg ctgggaatca atcaggctgt cgaggccaga gcccacaaaa ctgaaggcaa
     2941 aacccgccgg gatgattccg attaaggtgg cgctcacgaa tgtcttgagc cggacctggg
     3001 caagggccgc ggcgatgttg acaagccaga acggaaacac cggcgtaagc ctgaggaaga
     3061 ggagataact gaaggcatcc ctggcaaaac cctcctgcaa cttgcccacg atgccgccag
     3121 ccttgcgcga cagtgctgct tcaagtggtg ttctggctgc cagaaaaatt atc
//
LOCUS       DAORVN010000183         3164 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_26706_length_3164_cov_3.287231, whole genome shotgun sequence.
ACCESSION   DAORVN010000183 DAORVN010000000
VERSION     DAORVN010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3164)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3164)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3164
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26706_length_3164_cov_3.287231"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1923)
                     /locus_tag="PKV23_04740"
     CDS             complement(<1..1923)
                     /locus_tag="PKV23_04740"
                     /inference="COORDINATES: protein motif:HMM:NF017215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HQX84776.1"
                     /translation="MTAAESFFVGIDTGGTYTDAVIIAHGTQKVLGRAKALTTKGDLS
                     IGVKEALTMVMQDLPSAVKPSAIGLVSVSTTLATNAVVEGHGSPVAVFLIGFDSAMAS
                     RTGIAKAFPHVPLRMIAGGHDHNGDASAPLDVASVEREAALLRGEVSAFAVASAFAVR
                     NPEHENLARDIIMRVTGKPVTLSTELTSSLDAPRRALTAVLNARLISRISLLIDAVRL
                     AMAHLDISCPLMVVKGDGSLARAENVALKPIETVLSGPAASLVGAKWLSGLNDFIMSD
                     IGGTTTDIGILQNGRPQVAEEGAEVGGWRTMVKAIDVTTIGLGGDSEVHLAMDGSMQI
                     GPQRIVPISLIGARYPEVMAMLEGDLADTEGGSQQGRFVVAPFGRSAPVDHSGLTARE
                     IELLSKVTDRPKPLRKVAVSSSAQRALNTLRKKGLVQLCGFTPSDAAHVLGLQENWSK
                     PAALLAAQLVVRFRDMRLGTPERVEAFCREVWSETVRLSAKAILASALGIPVENNPLI
                     ESVVSGSPVKGLAAITISPTIPIVAVGGPARVYYAEVAKRLECEVVFVECAQHLAMEM
                     AREGGTTDDERIDYAFRRCVSRPPVEEERAALKELMQQQEKRFAEGWASPWQVATGKE
                     ARPEDLRVFIEEAAGVS"
     gene            complement(2013..2720)
                     /locus_tag="PKV23_04745"
     CDS             complement(2013..2720)
                     /locus_tag="PKV23_04745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX84777.1"
                     /translation="MFVSLLVAFLAVLCVSKRTATSACSNSSRAELRLFGRATLIFAG
                     VLGMAGTTAGGGPPVNPMSPASLRLVTLEEMPQADLQKDFAGKRLVVTAAGRRAKAES
                     ILARKHDLAELREQIRYELRLGNIAAEMAASLSRLIDDISSAASSTNENAPVWQISRG
                     QHEALAGLEQSQAQLNQTLADTIKLVQILGSVRGPEFSGTLVAAKIQVVKLLAEKAAV
                     DGSIDEIRQSLKHLKTG"
     gene            complement(2838..>3164)
                     /locus_tag="PKV23_04750"
     CDS             complement(2838..>3164)
                     /locus_tag="PKV23_04750"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HQX84778.1"
                     /translation="LLLHARALERKKYVEAIEHDDDFHRYIAGISNLPRLWRAIEISK
                     AQLDRCRHMMVPREGEAETTLEKHRTIITALKSGDPETAARAMRDHLETAYHSTVAVL
                     DGPGLN"
BASE COUNT          636 a         1022 c          872 g          634 t
ORIGIN      
        1 ggaaacacct gctgcttcct caataaaaac gcgtaaatct tccggccgcg cttcttttcc
       61 agtggcgact tgccaaggac tcgcccagcc ttcggcgaag cgtttttcct gttgttgcat
      121 caactccttg agcgccgcgc gttcttcctc gacaggcgga cggctcacgc aacggcggaa
      181 ggcgtagtcg atgcgttcgt cgtccgttgt gccgccttca cgtgccattt ccatcgcgag
      241 atgctgcgcg cactcgacga acacaacttc gcattccagc cgcttggcga cttccgcgta
      301 atagacccgc gccggcccgc caaccgccac aatggggatg gtgggtgaaa tcgtgattgc
      361 cgccaggcct ttcaccggtg agcccgaaac gacgctttca atcagcggat tgttctcgac
      421 cggaatgccc agcgccgaag ccaggatggc cttcgccgaa agccgcaccg tctcgctcca
      481 cacctcccgg cagaaggctt ccacccgttc cggcgtgcca agacgcatgt cacggaagcg
      541 caccacaagc tgggccgcca gaagcgccgc aggcttggac cagttttcct ggaggccaag
      601 cacatgtgcg gcgtcggaag gcgtgaagcc gcagagctgc accagtcctt ttttccgcaa
      661 ggtgtttagg gcccgctgcg cgctcgatga cacggcaact ttccgcagcg gcttcggccg
      721 gtccgtaacc ttggaaagaa gctcaatctc gcgcgccgtc aggccgctgt gatcgacagg
      781 tgcggatctg ccaaagggcg ccaccacaaa acgcccctgc tgggatccgc cttccgtatc
      841 cgcaaggtcg ccctcgagca ttgccatgac ttcaggatac cgcgcgccga tcagcgaaat
      901 gggcacaatg cgctgcggcc cgatctgcat ggaaccatcc atggcaagat gaacctcgct
      961 gtccccgcca aggcctatgg tcgtcacatc aatggccttg accatggtgc gccagccgcc
     1021 gacctcggcg ccctcttccg ccacctgggg tcttccattc tgcagaatgc cgatgtcggt
     1081 tgtcgtgccg ccaatatcgg acatgatgaa gtcattaagc cctgacagcc atttggcccc
     1141 aactaggctt gccgccggtc ccgaaagcac cgtttcgata ggcttgagcg ccacattttc
     1201 cgcccgcgcc agcgatccat cacctttcac caccatcagt gggcaggaaa tgtccaggtg
     1261 agccatggcg agccgcaccg cgtcaatcag aagcgagatc cgcgagatca gccgggcatt
     1321 cagaaccgcg gtcagtgccc ggcgcggcgc atcaagtgat gatgtcaatt ccgtagaaag
     1381 cgtcactggc ttgcccgtga cccgcatgat gatgtcgcgc gccaaatttt catgttcggg
     1441 attgcgtacc gcaaaagccg aggcaactgc gaacgccgat acttcgcccc tcaggagtgc
     1501 cgcctcgcgc tccaccgatg ccacatcaag aggcgcggac gcgtcgccat tatgatcatg
     1561 accacccgca atcatccgca gcggaacgtg cggaaaggcc ttcgcgatac cggtacggga
     1621 cgccatcgcg ctgtcgaagc cgatgaggaa aaccgccacc ggactgccgt gcccctcgac
     1681 cactgcattg gtggcaagtg tcgttgaaac ggacaccagc ccgatcgccg agggtttcac
     1741 agcggaagga aggtcttgca tcaccatcgt gagcgcctct ttcactccga tggaaagatc
     1801 gccctttgtc gttaaagcct ttgcccggcc aagaaccttc tgcgtgccat gcgcaatgat
     1861 cacggcatcc gtataggtgc cgcccgtatc aatgccgaca aaaaagcttt ccgctgcggt
     1921 catatctggc tttcaaggtt tggttggcca atgcttttac tgaaagcaac cgccatgtcc
     1981 accgccaggt catggacccg agggcgaatg tgctatccgg ttttgaggtg cttcaagctt
     2041 tgccggatct cgtcgattga cccatccact gccgccttct ctgccagcag cttcacgact
     2101 tgaatcttgg cagcgaccaa cgtcccggaa aactcagggc cacgcaccga acccagaatc
     2161 tgcaccagtt tgatcgtatc ggccagcgtc tggttcaatt gggcctgtga ctgttcaagc
     2221 ccggcaaggg cctcatgctg ccccctgctg atttgccaga caggtgcatt ctcgttggtg
     2281 ctactggctg cagaactgat gtcgtcgatt aggcgcgaaa gggaagcagc catttcggcc
     2341 gcaatgtttc ccagccgaag ctcatatctt atctgctcac gcagttctgc caggtcatgc
     2401 ttgcgcgcca gtatgctttc ggcttttgcc cgacggcccg cagcagtcac caccaagcgc
     2461 ttgccggcga aatccttttg caggtctgcc tgcggcatct cctccagcgt gacaaggcgc
     2521 agactggcag ggctcattgg attcaccggc gggccgccgc cggctgtggt tcccgccatg
     2581 ccgagcacgc ccgcaaaaat caacgttgcc cggccaaata atctgagttc ggcgcgcgat
     2641 gaattcgagc acgcgctggt agctgtccgc ttgctcacgc agagcaccgc cagaaacgcc
     2701 acaagcaacg aaacgaacac caaaaacgac atacgcaaac acaacccaca tggcacaaat
     2761 cctgaatgcg ccagccggca ccctaggaag gaacaatttc tttcgcgtta atgggaggac
     2821 ccagccccgt cctgccttca gttcaggccc gggccgtcga gcactgcgac cgtgctgtga
     2881 taggctgttt caaggtggtc gcgcatcgcc cgtgccgctg tttcaggatc gccgcttttc
     2941 agggcagtga tgatggtgcg atgcttctcc agtgtcgttt cggcttcgcc ctcgcgcggc
     3001 accatcatgt gacggcaacg gtccagctgc gcttttgaaa tctcaatggc ccgccagagc
     3061 cgcggcagat tgctgatgcc ggcgatgtag cggtggaaat catcgtcatg ttcaattgct
     3121 tcgacatact tcttgcgttc cagggcgcgg gcatgcagca ggag
//
LOCUS       DAORVN010000184         3152 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_26876_length_3152_cov_3.057475, whole genome shotgun sequence.
ACCESSION   DAORVN010000184 DAORVN010000000
VERSION     DAORVN010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3152
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26876_length_3152_cov_3.057475"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..246)
                     /locus_tag="PKV23_04755"
     CDS             complement(<1..246)
                     /locus_tag="PKV23_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HQX84779.1"
                     /translation="MGKVVLTFSMSLDGFIAGPDISLDNAMGVGGDRLHKWMFHGGPE
                     NRIDLGMARELLAKVGAVVLGKRTYDVGLQHWGDTPYP"
     gene            444..986
                     /locus_tag="PKV23_04760"
     CDS             444..986
                     /locus_tag="PKV23_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018063720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HQX84780.1"
                     /translation="MLKSTLVALALTLGASSAYAAMMVGGAEMFPAKNIIENAVNSKD
                     HTTLVAAVKAAGLVDTLSGAGPFTVFAPTNEAFAALPAGTVEGLLKPEAKDTLTKILA
                     CHVVPAKAMSDAVMKMVKDDGGAHMAKTVGGCMLNLKLDGDKVTVTDENGGVATVTIA
                     DVEQSNGVIHVIDKVLLPKT"
     gene            complement(1036..1335)
                     /locus_tag="PKV23_04765"
     CDS             complement(1036..1335)
                     /locus_tag="PKV23_04765"
                     /inference="COORDINATES: protein motif:HMM:NF015735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HQX84781.1"
                     /translation="MARWVAIFDDDPKALKLREDHHEAHFQYLALHAGKIRIAGGLRP
                     EPGGTFCGGLWVLEVESRAEAARLCEDDPYFRLGLRTRYRIYAWGKAPIYDDVIL"
     gene            complement(1345..2382)
                     /locus_tag="PKV23_04770"
     CDS             complement(1345..2382)
                     /locus_tag="PKV23_04770"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013421023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HQX84782.1"
                     /translation="MGYKVAVVGATGNVGREMLNILAEREFPVDECFAIASRKSMGVE
                     VSFGDKTLKCKDLEQFDFSQVDFVLMSAGSAVSKEWSPKIGAMGPIVIDNSSCWRYDA
                     EVPLIVPEVNAHALVEYMARNNRRNIIANPNCSTAQLVVALKPLHDVARIKRVVVSTY
                     QSVSGAGKEGLDELWNQTKGIFVTDQPTPKKFTKQIAFNVIPHIDVFMDSGETKEEWK
                     MVVETKKILDPKIRLTATCVRVPVFVGHSEAVNIEFEQPISAEEVREILREAPGCMVV
                     DKREDGGYVTPVECVGDFATFISRIREDSTIENGINLWVVSDNLRKGAALNTVQIAEC
                     LINRGLLKKAA"
     gene            2744..2833
                     /locus_tag="PKV23_04775"
     tRNA            2744..2833
                     /locus_tag="PKV23_04775"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2778..2780,aa:Ser,seq:cga)
     gene            complement(2882..>3152)
                     /locus_tag="PKV23_04780"
     CDS             complement(2882..>3152)
                     /locus_tag="PKV23_04780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX84783.1"
                     /translation="EGARNGTGRNAGYLARSSAGVITRLLQNSEHYYGQGVQAMKVTA
                     LSTEHFAEQPVGQIPELPIVTPGFSSHHVSRAGNLIAHDVRDRVR"
BASE COUNT          627 a          922 c          969 g          634 t
ORIGIN      
        1 gggataagga gtatcgcccc aatgctggag gcccacatca taggtccgtt tgccaagcac
       61 cactgcgccc acttttgcca gcagctcgcg cgccatgccg agatcgatgc gattttccgg
      121 tccgccatgg aacatccact tgtggagcct gtcgccgccc acgcccatgg cattatccag
      181 gctgatatcc ggcccggcaa tgaagccgtc cagcgacatc gaaaacgtca atactacctt
      241 acccacgctc acaccccaaa cttccgggaa gcgtatcaca ccttgggggc ggggaggcaa
      301 gaggagcatt acggcggcac ttcgattatg gatcaacccg tcgtgatcac gataacgttt
      361 ttaagccttc tgaaaccctg gggaaaactg atccgttcct gcgagtgttg gtcaaccgac
      421 aacattcaaa caggagattc cccatgttaa aatccacctt agttgcgctg gcgcttacgc
      481 ttggcgcatc gtctgcctac gcggccatga tggttggcgg cgccgaaatg ttccccgcca
      541 agaacatcat tgaaaacgcg gtcaattcca aggaccacac gacactggtt gcggcggtga
      601 aggctgcagg cctggttgac acgctttccg gcgccgggcc gttcaccgtg tttgcgccca
      661 ccaacgaagc ctttgctgcg cttcccgccg gcacggttga gggcctgctg aagccggaag
      721 ccaaggacac gctgaccaaa attctcgcct gccacgtcgt cccggccaag gccatgtcgg
      781 acgcggtgat gaaaatggtg aaggacgacg gcggcgcgca catggcgaaa accgtgggcg
      841 gctgcatgct gaacctgaag ctcgatggcg acaaggttac cgtcaccgat gaaaacggcg
      901 gcgttgccac cgtcaccatc gccgatgtgg aacagtcaaa cggcgtgatc catgtgattg
      961 acaaggttct tttgccaaag acgtaatacg gcggcaggag aagctggacc gggcattatt
     1021 acgcccggtc ctgttctata aaattacgtc atcatagatg ggagctttgc cccacgcata
     1081 aatccggtag cgggttctca ggccaagcct gaaataggga tcgtcctcgc agaggcgggc
     1141 cgcttccgcg cggctttcga cttcaagcac ccaaaggccg ccgcaaaatg ttccgccggg
     1201 ttcaggccgc aagccaccgg caatccgtat cttgccggcg tgaagcgcca aatactgaaa
     1261 atgcgcctcg tgatgatcct cgcgcaactt tagcgctttg gggtcatcat cgaagatagc
     1321 gacccagcgg gccatgggtt ttgctcaggc ggctttcttc agcaggccgc ggttgatcag
     1381 gcattcggcg atctgcaccg tgttgagggc agcacccttg cgcaggttgt cggagacgac
     1441 ccagagattg atgccgtttt cgatggttga atcctcgcgg atgcggctga tgaaagtggc
     1501 aaaatcaccg acgcattcaa ccggcgtgac gtagccgcca tcctcgcgct tgtcgacgac
     1561 catgcagccc ggcgcctcgc gcaggatttc gcgcacctct tcggcggaaa tgggctgctc
     1621 gaactcgatg ttcaccgctt ccgagtggcc gacgaagacc ggcacgcgca cgcaggtggc
     1681 ggtgagccgg atcttgggat cgagaatctt cttggtctcg accaccatct tccattcttc
     1741 cttggtctcg cccgaatcca tgaacacgtc gatatgggga atgacattga aggcgatctg
     1801 cttggtaaac ttcttcggcg tcggctggtc ggtcacaaag atgcccttgg tctggttcca
     1861 cagctcgtcg aggccttcct tgccggcgcc ggaaacggac tggtaggtgg acaccaccac
     1921 gcgcttgatc cgggccacgt catgcagggg cttgagggcg accacaagct gggccgtcga
     1981 gcaattggga ttggcgatga tgttgcggcg gttgttgcgg gccatgtact cgaccagcgc
     2041 atgggcgttc acttccggaa cgatgagcgg cacttccgcg tcatagcgcc agcaggagga
     2101 attatcgatg acgatggggc ccatcgcgcc gatcttcggc gaccattcct tggagacggc
     2161 ggagcccgcc gacatcagca cgaaatcgac ctgggaaaag tcgaactgct cgagatcctt
     2221 gcacttgagg gtcttgtcgc cgaaggagac ctcaacgccc atggatttgc gcgaggcgat
     2281 ggcgaagcac tcatcgaccg ggaattcgcg ctcggcgagg atgttgagca tttcgcggcc
     2341 cacattgccc gtggcgccga cgactgcaac cttgtaaccc atggaagtct ccttgactta
     2401 gtctctggtc cgcccgctct ggagcccttg tgcggctccg ctttcggcat agggggttcc
     2461 ggccaccacc ccccgagagc gatgaggccg ggggaggagg ctaaacggtt tttttagtcg
     2521 gttttttgaa agtggcggaa aggcaagccc ggcctgcgat tgcgggggca atcggggctg
     2581 aatgcatcgc tgaaacggcg aacgtcatga tgggcctttg tttcctggtt cgggcggttt
     2641 ctagcaggtc gcggggccct gtcaacgggg ggccgaattg gggcttggca gggccccgcg
     2701 tcttggctat tcagcggggg cgaccacagg ctttcgggca tgcggagagg tggcagagtg
     2761 gtcgatcgcg tcgcactcga aatgcgaagt acgcgcaagc gtaccggggg ttcgaatccc
     2821 tccctctccg ccagcaattc catgaaccag accaaggtcg cccggcgggg atgagcgccg
     2881 ttcagcgcac cctgtccctg acgtcgtgag cgatcagatt gcctgcccgg gagacgtggt
     2941 gggatgaaaa gccgggcgtg acgatgggga gttccggaat ttggccgacg ggttgctctg
     3001 cgaaatgttc ggtgctcaat gcggtcacct tcatcgcctg gacgccctgg ccatagtagt
     3061 gctcgctgtt ctgcaagagg cgggtgatca cgcctgcgga tgagcgagcc aaatatccgg
     3121 cgttgcgccc ggtgccattg cgagcgcctt ca
//
LOCUS       DAORVN010000185         3140 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_27083_length_3140_cov_3.201297, whole genome shotgun sequence.
ACCESSION   DAORVN010000185 DAORVN010000000
VERSION     DAORVN010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3140
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27083_length_3140_cov_3.201297"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..535
                     /locus_tag="PKV23_04785"
     CDS             <1..535
                     /locus_tag="PKV23_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009466796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HQX84784.1"
                     /translation="NPVTSRKIRQDDILSLNCFPMVAGYYVALERTLFAETASKEHLK
                     MWEMNCHVHDVGKKLLVPGAKCSDIAKSLNDIYAQHDLLKYRTFGYGHSFGVLCHYYG
                     REAGLELREDCDTVLEPGMVVSMEPMIMIPEGQKGAGGYREHDILVITEKGNRNITGF
                     PYGPEHLIVKAKKKKRH"
     gene            822..1910
                     /locus_tag="PKV23_04790"
     CDS             822..1910
                     /locus_tag="PKV23_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009464701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQX84785.1"
                     /translation="MVRFSQVQKSYDGETLVVKDLNLDIAKGEFLTMLGPSGSGKTTT
                     LMMLAGFEPATHGEIYVKGKPINNTPPHKRGIGMVFQNYALFPHMTVAENLAFPLQVR
                     NMGKAEIEDRVKNILKLVELPQMASRRPGQLSGGQQQRIAVARALVFEPDLVLMDEPL
                     GALDKQLREQMQYEIKHIHERLGVTIVYVTHDQAEALTMSNRIAVFNDGVVQQLSTPD
                     ELYERPQNSFVAQFIGENNKLKGVISDVDRDNIATVKLDSSDVVKALAVNVSAKGERT
                     LLSIRPERVEINPLAKNVDVLLRGKIAELIYLGDHIRARLEVAGHDNFIVKVPNKASN
                     EAISHGKTVNVGWSVKDCRALDFIELHH"
     gene            1929..2639
                     /locus_tag="PKV23_04795"
     CDS             1929..2639
                     /locus_tag="PKV23_04795"
                     /inference="COORDINATES: protein motif:HMM:NF018420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1194 domain-containing protein"
                     /protein_id="HQX84786.1"
                     /translation="MQHASFWKKRVVAAAFLLLALTTPSPVTRAQDAEVDLALVLAVD
                     CSFSVDFREFALQMRGLGEAFRKPEIKRAIAMGNLQRIAVSVVQWSDESNQVVAMPWT
                     ILESEEDGDLFGETLAAMPRKLAEGGTSISSALLYSAAHLSRAPRALRLVIDVSSDGR
                     NNVGVPVNAVRDRLVAQGITINALTILNEWPTLDIYFQNQVVGGEGHFVIPANDYGAY
                     ADAIYRKLLREITGPGIS"
     gene            2720..>3140
                     /locus_tag="PKV23_04800"
     CDS             2720..>3140
                     /locus_tag="PKV23_04800"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HQX84787.1"
                     /translation="MQPFTLSRLPEIIFGAGRIADLAGKVESLAGIGAAVLVVADPML
                     TKLGLTGRALESLKAAGLEAQVFDGWKGEPKAAEIDAAGAMARAMHAKAIVGLGGGSA
                     LDTAKLVAACAASGLAVQSYQLCETPLPKNILPIIAVP"
BASE COUNT          674 a          946 c          894 g          626 t
ORIGIN      
        1 caatccggtg accagccgca aaatcaggca ggacgacatt ctctcgctca attgctttcc
       61 catggtggcg ggttactacg ttgccctcga acgcaccctg tttgctgaaa ccgccagcaa
      121 ggaacacctc aagatgtggg aaatgaactg ccacgtccat gacgtgggca agaagctgct
      181 ggtgcccggc gctaaatgct ccgacatcgc caaatccctc aacgacatct acgcccagca
      241 cgacctgctg aaataccgca ctttcggcta tggccattcc ttcggcgtgc tgtgccacta
      301 ttatggccgc gaggcggggt tggagctgcg cgaggattgc gacacggtgc tcgagcccgg
      361 catggtggtg tccatggagc ccatgatcat gatccccgaa ggccagaagg gggcaggcgg
      421 ctaccgcgag cacgacattc tcgtgatcac cgaaaagggc aaccgcaaca tcaccggttt
      481 tccctatggc cctgaacacc tgatcgtgaa agccaaaaag aagaagcggc attagcgaaa
      541 tttcctctcc caaagggaga gcaaggggcc caagcgcagc ttgggaaggt gaggggttac
      601 ggtgccagaa agccaatact gcaacccctc atcctcccac cgcgttccgc ggcgggcctc
      661 gccttctcct tgcgggagaa ggaaagttgc agggcgagga acgggataca cactgcgcca
      721 atatcatcgc ttcccttttt tccctgaccg cataatatgg cctcaaaaag caagggcgag
      781 gcgataaact gatgaatact gctgctgcag ataatgaacc tatggtcagg ttttcccagg
      841 ttcaaaagag ctatgatggc gaaaccctgg ttgtgaagga tctcaacctc gatattgcca
      901 agggcgaatt cctcaccatg ctgggcccct ccggctccgg caagaccacc accctgatga
      961 tgctggcggg ctttgagccc gcaacccatg gcgaaatcta tgtgaagggc aagcccatca
     1021 acaacacccc gccgcacaag cgcggcatcg gcatggtctt ccagaactat gcgcttttcc
     1081 cccacatgac cgtggccgaa aacctggcct tccccctgca ggtgcgcaac atgggcaagg
     1141 ccgagatcga ggaccgcgtt aagaacattc tcaaacttgt cgagcttccg cagatggcga
     1201 gccgcaggcc tggccagctt tccggcggcc agcagcagcg cattgcggtt gcccgcgccc
     1261 tggtgttcga gcccgatctc gtcctgatgg atgaaccgct gggcgcgctc gacaagcagc
     1321 tccgcgaaca gatgcaatat gagatcaagc acatccacga gcgcctgggc gtgactattg
     1381 tctatgtcac ccacgaccag gcggaagcgc tcaccatgtc gaaccgcatt gccgtattca
     1441 atgacggcgt ggtgcagcag ctctccactc ccgatgaact ctatgagcgt ccgcagaatt
     1501 ccttcgtcgc ccagttcatt ggtgagaaca acaaattgaa aggcgtgatt tcggacgtgg
     1561 atcgtgataa catcgccacc gtgaagcttg attccagtga cgtggtgaaa gccctcgcgg
     1621 tcaatgtcag cgccaaaggt gagcgcaccc tgctgtcgat ccggcccgag cgcgtggaaa
     1681 tcaacccgct ggccaagaac gttgatgtat tgctgcgcgg aaaaattgcc gaactgattt
     1741 atctcggcga tcatatccgc gcccggctgg aagttgccgg tcacgacaat tttattgtga
     1801 aagtgcccaa caaggccagc aatgaggcca tctcccatgg caagacggtg aatgtgggct
     1861 ggtcggtgaa ggattgccgc gctttggact ttattgaatt gcaccattag cgggaggagg
     1921 tatcgcccat gcaacacgcc agcttctgga aaaagcgtgt tgtggccgca gcctttcttc
     1981 tgctggcatt gaccacgcca tcgcccgtca cccgcgccca ggacgccgaa gtcgacctcg
     2041 ccctggtgct tgccgtggat tgttccttca gtgtggattt ccgggaattt gccttgcaga
     2101 tgcgcggcct tggcgaggct ttcaggaaac ctgagatcaa gcgcgccatt gccatgggaa
     2161 acctccagcg catcgccgta agtgttgtgc agtggtccga tgaatccaat caggtggtgg
     2221 ccatgccgtg gaccattctg gaaagcgagg aggatggcga tcttttcggc gaaaccctgg
     2281 ccgccatgcc gcgcaagctg gccgagggcg gcacttcgat ttcaagcgcg cttctctatt
     2341 cggcagccca cctctctcgt gccccgcgcg cccttcgcct ggtgattgat gtgtcttccg
     2401 acgggcgcaa caacgtgggt gttccggtga acgcggtgcg cgaccgcctg gtggcccagg
     2461 gcatcaccat caacgcgctc accattctca atgaatggcc gaccctcgac atttatttcc
     2521 agaaccaggt tgtgggtggc gaaggccatt ttgtgattcc cgccaatgac tatggcgcct
     2581 acgccgatgc catctaccgc aagctgctgc gcgaaatcac cggcccagga atttcctgaa
     2641 tcaggtgaaa atgcctcacg cttgaccgct tcctgcggcg gccataagcg gatggaaacc
     2701 tcacgtcacg gaaatctcca tgcagccatt taccctttcc cgccttcctg aaatcatctt
     2761 tggggcaggg cgcattgctg atctcgcagg caaggtcgag agtctcgccg ggataggtgc
     2821 ggccgtcctt gtcgtggccg accctatgct cacgaaactg ggcttgaccg ggcgggcgct
     2881 ggaaagcctg aaagccgcgg gcctcgaagc ccaggttttt gacggctgga agggggaacc
     2941 aaaagccgcc gagattgacg cggccggcgc aatggcgcgg gcgatgcatg ccaaagccat
     3001 cgtcggcctg ggcggcggct ccgcccttga tacggcaaag ctcgtggccg cctgtgctgc
     3061 ctccgggctt gcggtccaga gctaccagtt gtgtgaaaca cccctgccca aaaacatctt
     3121 gcccatcatc gctgtgccta
//
LOCUS       DAORVN010000186         3107 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_27619_length_3107_cov_3.112713, whole genome shotgun sequence.
ACCESSION   DAORVN010000186 DAORVN010000000
VERSION     DAORVN010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3107
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27619_length_3107_cov_3.112713"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="PKV23_04805"
     CDS             <1..253
                     /locus_tag="PKV23_04805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX84788.1"
                     /translation="GLFYYVTRDNSLLYSTTDVIDTYVYRALRVHADIGMAAAAGLYH
                     ECGAYIWSHRNPRAIRQDHADALGARSVEHRFGKDSDGV"
     gene            189..725
                     /locus_tag="PKV23_04810"
     CDS             189..725
                     /locus_tag="PKV23_04810"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HQX84789.1"
                     /translation="MPTPWERDPWNIASGKTLTVFDVGITRIGLLICYDIEFPLLSRA
                     LAEAGAEIILAPSNTETEWGYWRVRTGAVARALENQVYTVHSPVVGASPWSAACPANC
                     GMAGIFAPSDVGFPPGGVLALGEMNKPQWVYAEVDLDLIAKVRASGGVQTYRHWPEQP
                     GASALPKAQVVDISGAKQ"
     gene            complement(708..1625)
                     /locus_tag="PKV23_04815"
     CDS             complement(708..1625)
                     /locus_tag="PKV23_04815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX84790.1"
                     /translation="MPICQRDPWRDQYFENVPCPDNVLIATDDFDAWPWYPKHNWIYD
                     KLRIAQSQGFNSGPHGVMPPAFPVFSKPITNLKGMGVGSRVVSSENEMLACSTPGHFW
                     MPLLEGAHVSTDCAVVSGQVKWLRHATGVPGPEGTFKYWTLHADEMPDVATYLKAWVG
                     KYMAAYTGMMNFETIGGKIIEAHLRFADQWCDLNGKGWIEAMVRLYADGVWDYDEQRR
                     VGYSVPLFARHNSNFSHPPQDLQTRIRAMPQVSSLQITFYDKKDAGLHPMPPGGFRLG
                     IVNAWDLQAGFTAVDELAKAFPTHSLLRA"
     gene            complement(1688..2398)
                     /locus_tag="PKV23_04820"
     CDS             complement(1688..2398)
                     /locus_tag="PKV23_04820"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HQX84791.1"
                     /translation="MYKTIFAAGALTLAAACLTPALADEGKLVRSVSLSGHGEVRMAP
                     DLAIVTVGVMSSAATAREALDANTKAMEAVMTSLTAAGIEARDIQTSNFSVNPRYDYG
                     QNNAQPPKVIGYDVSNNVSVTVRKLDSLGAVLDQVVSSGSNQINGVAFQVSKPEAATD
                     EARKLAVADALRKAQVYTAASGVTLGQIISLTEGGGYQPPQPVFKTMRAEAASADVPI
                     AQGEQVIAVDVNFTWEIK"
     gene            complement(2469..2882)
                     /locus_tag="PKV23_04825"
     CDS             complement(2469..2882)
                     /locus_tag="PKV23_04825"
                     /inference="COORDINATES: protein motif:HMM:NF016701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HQX84792.1"
                     /translation="MTISGGCLCKSVRISVTGAPLAARTCWCRVCQYLGAGNGAVSVC
                     FRTDAVSVDGDVNWFESIADSGNRMKRGFCPTCGTPLFSKALSRPHLIFIRAGALDDP
                     NLMAPQMTIWTDAAPDWACIDPDLLKVPGQPPPVA"
BASE COUNT          588 a          859 c         1022 g          638 t
ORIGIN      
        1 tggcttgttc tactatgtca cccgcgacaa tagtttgctg tactcaacca ccgacgtgat
       61 cgacacgtat gtgtatcggg cgctgcgggt gcacgccgac atcggcatgg cagcggcggc
      121 cgggctgtac catgaatgtg gcgcatatat ttggtcccac cggaatccgc gggcaatacg
      181 acaagatcat gccgacgcct tgggagcgcg atccgtggaa catcgcttcg ggaaagactc
      241 tgacggtgtt tgacgtgggg atcacgcgca tcggcctgct catctgctat gacatcgagt
      301 ttcccttgct gtcgcgggcg ctggcggaag cgggggcgga aatcatcctg gcgccatcaa
      361 atacggaaac cgaatggggc tattggcggg tgcgcaccgg ggccgtggcg cgggccctgg
      421 agaaccaggt ttacacggtg cattcgccgg ttgtcggcgc gtcaccctgg agcgcggcgt
      481 gcccggcaaa ttgcggcatg gcgggaatct tcgcgccatc ggacgtgggc tttccgccgg
      541 gcggcgtgct ggcgctgggc gaaatgaaca agccgcaatg ggtttatgcg gaggtcgacc
      601 ttgatctcat tgccaaggtg cgcgcctcgg gcggggtgca gacttacagg cattggccgg
      661 agcagcccgg ggcttccgca ttgcccaaag cgcaagtggt ggatatttca ggcgcgaagc
      721 agtgagtgcg tcgggaaggc cttggccagt tcatcgacgg cggtgaagcc tgcctgcaga
      781 tcccaagcgt tgacgatccc caagcgaaaa ccgccgggtg gcatgggatg gaggcccgca
      841 tcctttttgt catagaatgt gatctgcagg ctcgagacct gcggcatggc gcggatgcgg
      901 gtttgcaggt cctgtggcgg gtgggaaaag ttgctgttgt ggcgggcaaa gagtggaacg
      961 gagtagccca cgcggcgctg ctcgtcataa tcccagaccc catccgcata gaggcgcacc
     1021 atggcctcga tccagccctt gccgttgagg tcgcaccact gatcggcaaa gcgcagatgc
     1081 gcctcgatga tttttccgcc aatggtttcg aaattcatca tgccggtata ggcggccatg
     1141 tatttgccga cccaggcctt gagataggtt gcgacgtctg gcatttcatc ggcgtggagc
     1201 gtccagtatt tgaaggtgcc ttcgggcccc ggaacgccgg tggcgtggcg cagccatttg
     1261 acctgcccgc tcaccaccgc gcagtcagtt gaaacatgag cgccttcgag caaaggcatc
     1321 cagaaatggc ccggggttga gcaggcgagc atttcattct ccgaggaaac cacgcggctg
     1381 cccacgccca tgcccttcag gttggtgatg ggtttggaaa acaccggaaa ggccggcggc
     1441 atgacgccat gggggccgct gttgaacccc tggctttggg cgatgcggag cttgtcataa
     1501 atccagttgt gcttgggata ccagggccag gcgtcgaaat cgtcggtcgc aatcaggacg
     1561 ttgtccgggc acggaacatt ctcgaaatac tggtcacgcc agggatcacg ttggcagatg
     1621 ggcatggccg gaccctaaca gaagcctgct gtcaggcaat ggcagcagat tcacttctct
     1681 ttgacattta cttgatttcc caggtgaagt tcacgtcaac ggcaatcacc tgttcgccct
     1741 gggcgatggg cacgtcggcg gaggcggcct cggcgcgcat ggttttgaag acgggctggg
     1801 gtggctggta accgccgcct tctgtcagcg aaatgatttg ccccagcgtc acgccgctgg
     1861 cggcggtgta gacctgagcc ttgcgcaggg catcggccac ggcaagcttg cgggcctcat
     1921 cggttgcggc ttcgggcttt gacacttgga acgccacgcc gttgatctgg ttggaaccgg
     1981 aggaaacaac ctggtccaga accgcgccaa gactgtcgag cttgcgcacg gtgaccgaga
     2041 cattgttgga aacatcatag cctatgacct tgggcggctg ggcattgttc tggccgtagt
     2101 cgtagcgcgg gttcaccgag aagttcgagg tctggatgtc gcgcgcctca atgcctgctg
     2161 ccgtcagtga tgtcatgacc gcttccatgg ccttggtgtt ggcatcgagg gcttcgcgcg
     2221 ccgttgcggc ggaactcatc acccctacgg tgacgatggc aagatcaggc gccatgcgca
     2281 cttcgccatg gccggagagt gaaacgctgc ggaccagttt gccctcgtcg gcaagagccg
     2341 gggtgaggca ggcggcggca agggttagcg ccccggcggc gaaaatggtc ttgtacattt
     2401 tttaaggtct cccaatggct ttgcgggcgg tgatgtggcg aagaattgcg gctgcagtgt
     2461 gtttgcgtct aggctacagg cggcggctgg ccgggcactt tcagaagatc cggatcgatg
     2521 caggcccagt ccggcgccgc atcggtccag atggtcattt gcggggccat gagatttgga
     2581 tcatcgagtg cgccggcgcg gatgaagatc aggtgcggcc gggatagggc cttgctgaat
     2641 aatggcgttc cacaagtcgg gcagaatccg cgtttcatgc ggttgccact gtcggcaatg
     2701 ctctcaaacc aatttacatc gccgtccacg gataccgcgt cggtgcggaa gcatacgctt
     2761 actgcgccat ttccagcgcc caaatattgg cagacgcggc accagcaggt gcgcgccgct
     2821 aggggtgccc ccgttactga aatccgcact gacttgcaca ggcaaccgcc ggatatggtc
     2881 atggatcatc cttttcatta gttgctgcag aatagcgccg aagggattgt ccgggcaagg
     2941 ttcaggatca gaagggaatg ccctcgactt gatcgaggta actggataat tcgtcgatgc
     3001 aattccgcga tgttttcgcg tcgacgcttt cagcggccag ttacagggca gcgccgaagt
     3061 cggtgatggg ctggaagcca accatgccgc cggagccgct catgttg
//
LOCUS       DAORVN010000187         3101 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_27696_length_3101_cov_4.100131, whole genome shotgun sequence.
ACCESSION   DAORVN010000187 DAORVN010000000
VERSION     DAORVN010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3101
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27696_length_3101_cov_4.100131"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1203
                     /locus_tag="PKV23_04830"
     CDS             <1..1203
                     /locus_tag="PKV23_04830"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HQX84793.1"
                     /translation="DYTARLVDSWRVGDLEHPTPRQRGFDRFYGFVDGVMNYFSPHYM
                     MEDDGRVEVSPTEFYLTDAITDNAVKMIDECVTARQPFFMYLAHHAPHWPLHAQENDI
                     AKYDGVYTRGWDAVRGARHEEMQARNILLHKWDISRRDDSAMAWQDIKAKDWESRRMA
                     VYAAMIDRLDQNIGRVLTRLKQHGIHDDTLILFLSDNGGCAEFMAEDGWGKFYPDHHN
                     DGRKIEIGNRPNLRPGGPLTYMSYDLPWANVSNAPFRLFKHWVHEGGISTPLLAQWPA
                     RFKPGGVAHTPAHVVDIMPTILEAAGVSYPREVGGHAIQPADGESLMALLDGKAWTRA
                     QPIYWEHEGNCAVRIGNFKLVRQFGKDWELYDMEKDRTELHDLAGKNHPLETQLKQEY
                     SSCQAHRR"
     gene            complement(1289..2176)
                     /locus_tag="PKV23_04835"
     CDS             complement(1289..2176)
                     /locus_tag="PKV23_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009759463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HQX84794.1"
                     /translation="MDWDKLRIFHAVADAGSFTHAGHELALSQSAVSRQISALETDLN
                     VPLFHRHARGLILTEQGEVLYRTAHDVFTKLAAAKTRLMDSKEKPSGELRITTTVGLG
                     SVWLTPRIKEFTELYPQISVTLLLEDRELDLSMREADVAIRLRRPTQPDLIQRKLFVV
                     HHHVYASTDYIKQHGIPKSIEDLDRHKILTYGQAPGYLTTINWLERAGRSEDNPRPVA
                     LRVNNAYGLRRAVQAGVGIASLADYIVAADNNLVQIDLPIELPQFDSYFVYPEELKET
                     KRITAFRDFIVSKAKEWSF"
     gene            complement(2182..>3101)
                     /gene="trxB"
                     /locus_tag="PKV23_04840"
     CDS             complement(2182..>3101)
                     /gene="trxB"
                     /locus_tag="PKV23_04840"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009764608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HQX84795.1"
                     /translation="AGYTAAIYAARAMLKPVLIQGIQPGGQLTITTDVENYPGFAKVI
                     QGPWLMEEMKAQAEHVGTEVISDTITEVRLKDKPIWLKGDSGQVYTCDALIICTGAQA
                     KWLNLPSEENFKGFGVSACATCDGFFYRDRKVLVIGGGNTAVEEALFLTNFASEVTVV
                     HRRNEFKAERILQNRLFAHPKIKVIWDSVLDDVLGTSDPKGVTGARLKNVKTGATQDV
                     AADGVFIAIGHKPATELFEGQLPFKQGGYLIVGNNSMATAIPGVYAAGDVTDDIYRQA
                     VTAAGMGCMAALEVEKYLHATEQVRKAAE"
BASE COUNT          635 a          833 c         1004 g          629 t
ORIGIN      
        1 gactacaccg cccggctggt tgatagctgg cgtgtcggcg atctcgagca tccgacgccg
       61 cgccagcgtg ggttcgaccg cttctacggg tttgtcgacg gggtgatgaa ttacttttca
      121 ccgcactaca tgatggaaga tgacggccgc gttgaagtat cgcccactga attctacctc
      181 accgacgcga tcaccgacaa tgcggtgaag atgatcgacg aatgtgtaac ggcaaggcag
      241 ccattcttca tgtatcttgc gcatcatgcg ccgcactggc cgttgcatgc gcaagaaaat
      301 gacattgcca agtatgatgg agtctatacc cgtggctggg atgcggtccg gggggcgcgg
      361 catgaggaaa tgcaggcgcg caatattctt ttgcacaaat gggatatttc ccggcgagat
      421 gatagcgcaa tggcgtggca agacatcaaa gccaaggatt gggaatcccg gcgcatggcc
      481 gtctatgcgg cgatgattga ccgcctcgac caaaacatcg gccgggtgct cacgcggctg
      541 aagcaacacg ggattcatga cgacacgctt attctgttcc tatccgacaa cggcggctgc
      601 gccgagttca tggcggagga tggctggggg aaattctatc ccgaccacca caatgatgga
      661 cgcaagattg aaatcggcaa ccggccaaat ctcagacctg gcgggccgct cacctacatg
      721 agctacgacc tgccctgggc caatgtctca aacgcgccgt tccggctgtt caagcattgg
      781 gtgcatgaag gtggcatatc aacaccactg ctggcccagt ggcccgcgcg ctttaaacca
      841 ggcggggttg cccatacgcc tgcccatgtg gtggacatca tgccaaccat attggaggcc
      901 gccggtgtct cataccccag ggaggtgggt ggccatgcca ttcaaccggc ggacggagaa
      961 agcctgatgg ctcttttgga tggcaaggcc tggacccgtg cccaacccat ctattgggaa
     1021 cacgagggca attgcgcggt acgaataggg aatttcaagc tggtccggca atttggcaag
     1081 gactgggaac tctatgacat ggagaaggac cgcactgaac tgcatgacct cgccgggaaa
     1141 aatcatcctc tagagacgca gctcaagcaa gaatattcgt cctgccaagc gcatcggcgt
     1201 tgaggactgg gacaagcttc ttccgcagct caagaaattg tgggagatgg acgatattca
     1261 cggttgagcc gtgtgaggag cgcctaagct agaagctcca ttccttcgcc ttcgacacga
     1321 tgaagtcgcg gaaggcggtg atgcgtttgg tttccttcag ctcttcggga tagacgaagt
     1381 aactgtcgaa ctgcgggagc tcgatgggga gatcgatctg cacgaggttg ttgtcggcgg
     1441 cgacgatgta gtcggcaagc gaagcaatgc cgacaccggc ctggacggcg cggcgcaggc
     1501 cataggcgtt gttgacgcgg agcgccacgg ggcggggatt gtcctcggag cggccggcgc
     1561 gctccagcca gttgatggtg gtgagatagc cgggagcctg gccgtaggtc aggatcttgt
     1621 gccggtccaa atcctcaatg gatttcggga tgccatgctg cttgatgtag tcggtcgagg
     1681 catagacgtg atgatgaacg acaaagagct tgcgctggat gagatcgggc tgggttggcc
     1741 ggcgcaggcg gatggcgaca tcggcctcgc gcatggagag atcaagttca cggtcctcaa
     1801 gcagcagtgt cacgctgatc tgcggataga gctcggtaaa ttccttgatg cgcggcgtga
     1861 gccagacgga gcccaggcca acggtggtgg tgatgcgcaa ctcgcccgag ggtttttcct
     1921 ttgaatccat caggcgggtt ttggccgcag ccagcttggt gaagacatca tgggcggtgc
     1981 gatacagcac ttcgccctgt tcggtgagga tcagcccacg ggcatggcgg tggaacagcg
     2041 gcacgttgag atcggtctca agggcgctga tctggcggct gacggcggac tgcgacaggg
     2101 caagctcatg gcccgcatgg gtaaagcttc cggcgtcggc gaccgcgtga aaaatcctga
     2161 gcttgtccca atccatggct gctactcggc agccttgcgg acttgctcag ttgcatggag
     2221 atatttttcc acttcgagag ccgccatgca gcccatgccg gcggcggtga cggcctggcg
     2281 gtagatatca tcggtgacat cgcccgccgc atagacgccg gggatagcgg ttgccatgga
     2341 gttgttgccg acgatgagat agccgccctg cttgaagggc agttgcccct cgaacaattc
     2401 ggtggcgggc ttgtggccga tggcgatgaa gacgccgtcg gcggcgacat cctgggtcgc
     2461 gccggttttc acattcttga ggcgggcgcc ggtaacgccc ttgggatcgc tggtgcccaa
     2521 gacatcatcg agaaccgagt cccagatgac tttgatcttg ggatgggcga agaggcggtt
     2581 ctgcaggatg cgctcggcct tgaattcgtt gcgccggtga accacggtga cttcgctggc
     2641 gaaattggtg aggaagaggg cttcttcgac cgcggtgttt ccgccgccga tgaccagcac
     2701 cttcctgtcg cggtaaaaga agccgtcgca ggtggcgcag gcggaaacgc cgaagccctt
     2761 gaaattctct tccgatggca ggttcagcca cttggcctgg gccccggtgc agatgatcag
     2821 cgcatcgcag gtatagacct ggccgctgtc gcccttcaac cagatgggct tgtccttcag
     2881 gcggacctcg gtgatggtgt cggaaatgac ttcggtgccg acatgctcgg cctgggcctt
     2941 catttcctcc atcagccagg ggccctggat gactttggca aagccggggt agttctcaac
     3001 atcggtggtg atggtgagct ggccgccggg ctggatgcct tgaataagga caggcttcaa
     3061 catggcgcga gcggcgtata ttgcagcagt ataacccgca g
//
LOCUS       DAORVN010000188         3100 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_27712_length_3100_cov_4.076519, whole genome shotgun sequence.
ACCESSION   DAORVN010000188 DAORVN010000000
VERSION     DAORVN010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3100
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27712_length_3100_cov_4.076519"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1485)
                     /locus_tag="PKV23_04845"
     CDS             complement(<1..1485)
                     /locus_tag="PKV23_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013532890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HQX84796.1"
                     /translation="MSDIAQSAPSTSNGRWLGLWIVVGAIVITAILLPVPEALSPLTK
                     YPDALVVPFNDWVGAAMAWLKTNFTWLTRWLAGLIEVPLRFAFNLLAKGFKFGTGEAA
                     WALPRLSWLGVVLVMAWLGYIFGGLRLALLCGLGFLSLVLFDVWNNSMLTLALIIICV
                     PFCIVTGLAFGIWGHFSPRVNRWLISPALDLMQTMPTFAYLIPMLLLFGASPVSALLA
                     TGIFATPPMVRATVLGLNKVPQEIKDFADMAGCTKSQKLWRVLLPSAKPSLLIGVNQV
                     IMLALNMVIISSMIGAGGLGYDVLLALRALKIGQAMEAGLAIVIIAVVLDRLSQAIAV
                     KRPEAADARSFWARHTNFVLAIAVLLVTTALSLVFPALHTLPDSLTFTTAPYWKAGVD
                     WITINFFDTIEAMRVFLLLNFLNPLRSGFEALPWFGVLLVLTALAWRLGGWGLALIIA
                     VLTAFCATTGLWVPAMQTLYLVGFSALVASALGIPIGLWCSRSDR"
     gene            complement(1482..2546)
                     /locus_tag="PKV23_04850"
     CDS             complement(1482..2546)
                     /locus_tag="PKV23_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006330157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HQX84797.1"
                     /translation="MKTKTPDAESQRPVKLSCRNLWKVFGPNAGKFMAERKGTATAAE
                     LDEAGLVGAVRNTTLDVREGEIFIVMGLSGSGKSTLVRCLSRLIEPTTGELSFDGKDL
                     LKASEAELIEIRRHKMGMVFQHFALLPHLTVLENVAFPLSIQGIGRAEREARAQQMIE
                     LVSLNGREHHFPSELSGGQQQRVGIARSLAVEPEIWFLDEPFSALDPLIRREMQTELM
                     RLQSVLKKTVVFITHDFDEAIRLADRIAIMKDGEVIQIGTPEDLVNNPATAYVAEFTA
                     DVNRAKVLSARALMKPATAKSAGLKKVSARAKIASFAAAIVDSARPFAVIGDSGEVIG
                     EISPKSVINLLSGKDRGANA"
     gene            complement(2569..>3100)
                     /locus_tag="PKV23_04855"
     CDS             complement(2569..>3100)
                     /locus_tag="PKV23_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008877828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HQX84798.1"
                     /translation="AEAFATAETAPKGRYVGGPVTWEGFDDERVEALGLPFTVIHAGT
                     DAAMFAELDSAYQRKAPVMVWVYSPHWAPAKYEGEWVEFPEYTPECYTDPKWGSNPDK
                     AYDCGKPFGEIWKFGWAGMKDKWPVAYKVAKAYQIDTNELNAMSGQIDLDGKSIEEVA
                     AAWVAANEAKWKAWAQ"
BASE COUNT          668 a          915 c          978 g          539 t
ORIGIN      
        1 ccggtcgctg cgcgagcacc acaggccgat gggaatgccg agggcggaag ccacaagcgc
       61 ggaaaagcca acgagataaa gcgtctgcat ggcgggaacc caaagtccgg ttgtggcgca
      121 gaaggcagtg aggacggcga tgatcagggc cagcccccag ccgccaaggc gccaggccag
      181 ggcggtcagg acaagcagca cgccgaacca cggtaaggcc tcaaagccgg aacgcagggg
      241 gttcaggaaa ttgagcagta ggaacacccg catggcttca atggtgtcga agaaatttat
      301 ggtgatccag tcaacgccgg ccttccagta aggcgcagtg gtgaaggtga gcgaatcggg
      361 cagcgtgtgg agcgccggga aaaccaggct gagcgccgtg gttacaagaa gcacggcaat
      421 ggccagaaca aagtttgtgt ggcgggccca gaacgaccgg gcatccgccg cttccggtcg
      481 cttcacagca atggcctggc tgagccggtc aagcaccacc gcgatgatga caatggcaag
      541 gcccgcctcc atggcctggc cgatcttaag ggcgcgcagc gccagcagca catcatagcc
      601 caggcctccg gcgccaatca tcgacgagat gatgaccatg ttgagggcca gcatgatgac
      661 ctggttcacc ccaatcagca gggagggttt ggccgacggc agcagcacgc gccaaagctt
      721 ctgcgatttg gtgcagcccg ccatgtcggc gaaatccttg atctcctggg gcaccttgtt
      781 gaggcccagc acggttgcgc gcaccatggg cggggtcgca aaaatgccgg tggcaagcag
      841 ggcggatacg gggctggcgc cgaacagcag cagcatcggg ataagatagg cgaaagtcgg
      901 catcgtctgc atgaggtcga gggcgggcga aatcagccag cggttcacgc gcggcgaaaa
      961 atgcccccag atgccgaagg ccaggccggt gacgatgcag aacggcacgc aaatgatgat
     1021 gagcgccagg gtgagcatgg aattgttcca cacatcgaaa agcacgagcg agaggaaccc
     1081 caggccgcag agcagcgcca ggcggaggcc gccaaaaata tagcccagcc aggccatgac
     1141 caggaccacg ccaagccacg acaaccgcgg cagggcccag gcggcctcgc ccgtgccgaa
     1201 tttgaagccc ttggcaagca ggttgaacgc aaagcgcagg ggtacttcaa tgagcccagc
     1261 aagccagcgg gtgagccagg tgaaattcgt cttgagccag gccatggcgg cgccgaccca
     1321 gtcgttgaat ggaacgacca gggcatcggg atatttggtc aagggtgaga gcgcctcagg
     1381 aaccggcagg agaatggcgg tgatgacaat cgcacccacg acaatccaaa ggccaagcca
     1441 gcggccattg gacgtcgaag gggctgactg ggcgatatca ctcatgcgtt tgctccccgg
     1501 tcctttccgg aaagcagatt gataaccgac ttcggcgaaa tttcgccaat cacttcgccg
     1561 gagtcgccga tcacggcaaa tggcctggct gaatccacga ttgccgcggc gaaactggca
     1621 atcttggccc tggccgaaac ttttttcagt ccggcggatt tcgccgttgc cggcttcatc
     1681 agggcgcggg ccgacagcac cttggcgcgg ttcacatcgg cagtgaattc cgccacatag
     1741 gcggtcgccg gattgttgac gagatcttcc ggcgtgccga tctggatgac ttcgccatcc
     1801 ttcatgatgg caatgcggtc ggccagcctt atggcctcat cgaagtcatg ggtgatgaag
     1861 accacggtct tcttgagaac gctttgcagg cgcatcaact cggtctgcat ctcgcggcgg
     1921 atcagcggat cgagggcgga gaacggctcg tcaagaaacc agatctcagg ctccacggcc
     1981 agcgaccggg caatgccgac gcgctgctgc tggccgcccg aaagctctga cggaaaatga
     2041 tgctcgcgcc cgttaaggct gacaagttca atcatctgct gggcgcgggc ttcgcgctcg
     2101 gcccggccga tgccctggat cgacagtggg aaggcgacgt tttccagcac cgtgagatgg
     2161 ggcagcaggg cgaaatgctg gaacaccatg cccatcttgt ggcggcggat ttcgatgagt
     2221 tcggcctcgc tggccttcag caggtccttg ccgtcgaagc tcagttcgcc ggtggtgggc
     2281 tcgatcaggc gcgacaggca gcgcaccagc gtcgacttgc cggatccgga aaggcccatg
     2341 acgatgaaaa tttcgccttc gcgcacatca agcgtcgtgt tgcgcaccgc accgaccagg
     2401 ccagcttcgt ccagctcggc ggcggtcgcc gtgcctttgc gctcggccat gaatttgccg
     2461 gcgttggggc caaacacttt ccacaaattg cggcaagaca gtttgaccgg acgctgggat
     2521 tcagcgtccg gtgttttcgt tttcatgcgt attgcccctg atacaggctt attgagccca
     2581 ggctttccat ttcgcttcgt tggcagcaac ccacgcagcg gcgacctctt cgattgactt
     2641 gccatccagg tcaatctggc ccgacatggc gttcagttca ttggtgtcaa tctgataagc
     2701 cttggccacc ttgtaggcaa ccggccactt gtccttcatg ccagcccagc cgaatttcca
     2761 gatttcgccg aacggcttgc cgcaatcata ggccttgtca gggtttgagc cccacttcgg
     2821 gtcggtgtag cattccggcg tgtattccgg gaattcaacc cattcgcctt cgtattttgc
     2881 cggagcccaa tggggcgaat agacccaaac catgacgggc gccttgcgct gataggccga
     2941 gtcaagctcg gcaaacatcg cagcatcggt gccggcatgg atgacggtga aaggcagtcc
     3001 gagagcctca acgcgctcat cgtcaaagcc ttcccaggtg accgggccac cgacataacg
     3061 gcccttgggg gcggtttccg ccgtggcgaa ggcttcagcg
//
LOCUS       DAORVN010000189         3089 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_27919_length_3089_cov_3.218194, whole genome shotgun sequence.
ACCESSION   DAORVN010000189 DAORVN010000000
VERSION     DAORVN010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3089
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27919_length_3089_cov_3.218194"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..741)
                     /locus_tag="PKV23_04860"
     CDS             complement(<1..741)
                     /locus_tag="PKV23_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008877390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HQX84799.1"
                     /translation="MADVKRGRDWGLPFAGIPGPFNAITDVAGVEVGTTTLISGEGPL
                     VVGNGPIRTGVTAVLPRGRAKAHIPCAAGYYSLNGNGEMTGTVWIEESGELQTPITIT
                     NTHSCGVTRDATIRWMVANRIGIGQDWGLPVSAETYDGDLNDINGFHVTAEHTIAALD
                     AARGGAVEMGSVGGGTGMICYDFKAGNGSSSRVISVDGSTYTVGVFVQANFGAREQLM
                     VLGVPIGPQITAEKLRGRDQGSVIAVVAT"
     gene            830..2626
                     /locus_tag="PKV23_04865"
     CDS             830..2626
                     /locus_tag="PKV23_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010685599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HQX84800.1"
                     /translation="METAGIGQYREFLIILGTAGLVIPLFVRLGISSILGFLLVGFLL
                     SRDVLGSFTSAFPFLQALVITDEEAVSSLGELGVVFLLFLIGLELSFERLVTMKRLVF
                     GLGSLQVISTTALVSLAAYALDFSPAQSLVIGTAFSLSSTAIVIQLYSNDKRLGSQSG
                     RTSFAILLFQDLAVVPILLMVGVLAHETSGPVLQSLAIAIAQAMLAVGLIVGVGRFAL
                     RPLLRMVASTNSSDLFMAAVLLIAVGTGVLANYAGLSMALGAFIAGLTLAETEYRRAI
                     EAVIEPFKGLLLGAFFLLVGLGIDLDGVVESPVQIIAIAAALIVAKTAVTYLFARAMK
                     VNHAAALETALLLGPCGEFAFVILSSAAATGILDRAIFSKLLLIVSLTMAAIPFLAIL
                     GKQLVKRSKRLQGNSPETLEAPPADDQPRIIVAGFGRVGHLIGSMLDEHKIPYIAVDA
                     DAALVTRERRAGFPVYYGDAANVEFLKKCGLADAKAIAITMDNPVRVDDVARAARAES
                     ADLKIIARARDERHAMRLYAAGVTEAVPETIESSLQLGESLLVEAGIAMGLVIASVHE
                     RRDTFRKLLGRPNRKKELDLERKRLRREKVLP"
     gene            complement(2636..>3089)
                     /locus_tag="PKV23_04870"
     CDS             complement(2636..>3089)
                     /locus_tag="PKV23_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016920645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HQX84801.1"
                     /translation="PDSVKKMRELVAAGKMQMKLGQVTGLTGSDGILSSVQVKGNDGA
                     AFDIQANTLLPFFGLTMKLGPVANWGLNLHENLIPVDTEKFESSTPGIFAIGDINHYP
                     GKLKLILSGFHEAALLAQQAFRYINPGERLLFQYTTSSTNLQKKLGVA"
BASE COUNT          579 a          975 c          834 g          701 t
ORIGIN      
        1 ggtcgcgaca acggcaatca ccgaaccctg atcccgcccc cgcagctttt cagccgtgat
       61 ctgcggcccg atgggaacgc ccagaaccat caactgttcg cgcgccccga aattggcctg
      121 aacaaatact ccaaccgtat atgtcgatcc atcaacgctg atcacgcgcg acgatgagcc
      181 attgcccgct ttgaaatcat agcagatcat ccccgtgcca ccaccgacgc ttcccatttc
      241 aacggctccg ccacgggccg cgtccagtgc cgcgatggtg tgttcagcag tcacatggaa
      301 tccattgatg tcattcaggt cgccgtcgta agtctcagcg gaaaccggca agccccagtc
      361 ctggccaatt ccaatcctgt tggccaccat ccagcggatt gtcgcgtccc gcgtcacgcc
      421 gcaggaatgg gtattggtga tcgtgatggg ggtctgcaat tcgcccgatt cttcgatcca
      481 cacggttcca gtcatttccc cgttgccgtt gagtgaataa tagccggctg cacagggaat
      541 atgggccttg gcgcggccgc gcggcagaac agccgtaacc cctgtccgaa ttggcccatt
      601 gcccaccacc agtggaccct cgccgctgat cagcgtggtc gtgccgactt caactccggc
      661 cacatcggtg atcgcattga atggcccagg tattcccgca aaaggcagtc cccagtcccg
      721 cccccgcttc acatcagcca tgaatgcctc ctcccccctc aatttgtaac tttttgcttt
      781 gcaaatctgg tctcttgcaa cactatggcg gtagcggtgc acagggtcca tggaaacagc
      841 aggcataggt caatatcggg aattcctgat catacttggc acagccggat tggtcatccc
      901 cctgttcgtg cgcctcggca tcagttccat attgggcttc ctgctagtcg gatttctcct
      961 cagtcgcgat gttctgggct cctttacctc ggcatttcct tttcttcagg ccctggtcat
     1021 aactgatgag gaggcggtct cctcgctcgg cgaactgggt gtcgtgttcc tgcttttcct
     1081 gatcggactc gaactctcct tcgaacggtt ggtcaccatg aagcgcctgg ttttcggcct
     1141 cggcagcctg caggtgatta gtaccacggc gcttgtctcg ctggcagctt acgccttgga
     1201 tttctctccc gcccaatccc tcgtcatcgg cacagctttt tcgctgtcat ccaccgccat
     1261 cgtcatccag ctctattcca atgacaaacg cctgggctcc caatcgggcc ggaccagctt
     1321 tgccatcctg cttttccagg atctggcggt agtccctatc ctcttgatgg ttggtgtttt
     1381 ggcgcacgaa acctccggcc ctgtcctgca gagcctggca attgccatag cccaggcgat
     1441 gctggctgtg ggcctgatcg tcggggtcgg tcgtttcgcg ctacggccac tactgcgaat
     1501 ggtagccagc acaaacagct ccgatttgtt catggcggcg gttcttctca tcgccgttgg
     1561 caccggggtg cttgcgaact atgccggcct ctccatggcc ctgggcgcct tcatcgcagg
     1621 attgacactg gccgaaaccg aataccgccg cgccattgag gccgtcatcg aaccattcaa
     1681 gggtcttctg ctgggcgcct tcttcctcct ggtgggcctc ggcatcgacc ttgatggcgt
     1741 cgtggaatcc ccggttcaga tcattgccat cgcagcagcc ctgattgttg cgaaaacagc
     1801 ggtgacctat ctctttgccc gcgccatgaa ggtcaaccac gccgcagcac tggaaaccgc
     1861 cctgctcctc ggcccctgtg gtgaatttgc ttttgtcatt ttgagttcgg ccgccgccac
     1921 gggtattctc gacagagcca ttttcagcaa gctgttgctg attgtatcgc tcaccatggc
     1981 ggccatcccg tttctggcaa tactcggcaa gcaactcgtc aagagatcca aaaggctgca
     2041 gggcaattct ccggaaacgc tggaagcgcc cccggcagat gaccagccac gcatcatcgt
     2101 cgcaggcttc ggtcgcgtgg gtcatctcat cggctccatg ctcgacgaac acaagatccc
     2161 ctacatcgcc gtggatgccg acgcagccct ggtgacacgt gagcgccggg ccgggtttcc
     2221 ggtctactat ggtgatgccg ccaacgtcga gtttctgaaa aaatgcgggt tggccgatgc
     2281 caaggccatc gccattacca tggacaatcc tgtgcgcgtt gacgacgtag cgcgtgccgc
     2341 gcgcgccgaa agcgccgatc tcaagatcat tgcccgcgcc cgcgacgaac gccatgccat
     2401 gcgcctttac gccgctggcg tgaccgaggc cgtgcctgaa accattgaat caagcctgca
     2461 actcggcgag tctctgctgg tggaagcggg cattgctatg ggactggtca tcgcctcggt
     2521 tcacgaacgc cgcgatacct tcaggaagct gctcggccgt cccaaccgca agaaggaatt
     2581 ggacctggaa cgcaaacggc tgcgtcgtga gaaggtgttg ccttgaagtg gcagcctatg
     2641 cgacgccaag cttcttctgc aggttcgtgc tcgacgtcgt gtactgaaac agcagtcttt
     2701 cgccgggatt gatatagcgg aacgcctgtt gtgccagcag cgccgcctca tggaagccag
     2761 acaggataag tttcagcttg cccggatagt ggttgatgtc gccgatggca aaaatcccgg
     2821 gcgtagaaga ctcgaatttt tccgtgtcga cgggaatgag gttttcgtgg agattgagcc
     2881 cccaattggc aaccggccca agcttcatgg tgaggccgaa gaacggcaga agcgtgttcg
     2941 cctgaatgtc aaaggcagcg ccgtcattcc ccttgacctg gaccgaggac aggattccat
     3001 cggaccccgt cagcccggtc acctggccaa gcttcatttg cattttgcct gcggccacaa
     3061 gctcccgcat ttttttcacg ctgtcgggt
//
LOCUS       DAORVN010000190         3082 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_28038_length_3082_cov_2.644863, whole genome shotgun sequence.
ACCESSION   DAORVN010000190 DAORVN010000000
VERSION     DAORVN010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3082
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28038_length_3082_cov_2.644863"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..634)
                     /locus_tag="PKV23_04875"
     CDS             complement(<1..634)
                     /locus_tag="PKV23_04875"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQX84802.1"
                     /translation="MANYFEQVLQNARKQGKVKTSKASPAEPPQGRLDGLVALMNQRR
                     LKEAVQQATAMAAEYPNAAIVFNILGAAQMGLRNLDGAAASFGKAVQLRPGFAPAHNN
                     LGVALKDLGRLQEAAASFIKAVQLKPDFAEAHSSLGTALHRQGLLDEAIASYDKALQY
                     KPDLAEAFNNRGAALKDRGRLEEAIASFGTALRLKPDYSEAHNNLGLALLA"
     gene            complement(648..1568)
                     /locus_tag="PKV23_04880"
     CDS             complement(648..1568)
                     /locus_tag="PKV23_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020186599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HQX84803.1"
                     /translation="MILITNVDDTGYAMIANLDVDLLKTFLAIADTGSFTRAAEEVHK
                     TQSAISMQMKRLEELLGVALFAKDGRMSRFTPDGERLVDYARRIVSFNDEVVSAFTKP
                     ELTGTIRFGTPDDYAERFLPEILARFARTHPLITVDVDCVGSGELFARVKRSEIDLAL
                     VTHGCDIITDEPVRREQLVWVTSMRHAAHMAEVLPLAVSHAGCEWRSKVLKALDKQQR
                     KYRVAYSTPNSNAVNAAVMQGLAVGAMPELCVRPGMRVLTEKDGFPPLGSFDIGLVRK
                     PGKSNKAIDALARHVSESLSNYQPQMVAAE"
     gene            1698..1955
                     /locus_tag="PKV23_04885"
     CDS             1698..1955
                     /locus_tag="PKV23_04885"
                     /inference="COORDINATES: protein motif:HMM:NF018294.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HQX84804.1"
                     /translation="MRDYALNQALSRGEYGIVYFLRRVTRNWKSKRRIASLGNFDDYM
                     LRDIGVTRDEVQWAAGLPLTVNAAVALEERALRRRKSGRLE"
     gene            2119..2310
                     /locus_tag="PKV23_04890"
     CDS             2119..2310
                     /locus_tag="PKV23_04890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX84805.1"
                     /translation="MMIDPKGSQPEALAKFAGVARHADGKPLREGVPHRHQGAKNAIN
                     KLPDRTAKNGKGRGGARSR"
     gene            2345..2818
                     /locus_tag="PKV23_04895"
     CDS             2345..2818
                     /locus_tag="PKV23_04895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX84806.1"
                     /translation="MQTVFAALAILIATAPAHAASLFDTPGSNPAANRCDLFDPNCKP
                     RQLFVRPPEPVTKNNPLGAPAPKLILPRDQNDRSAKPSPLLHVPGQPSRRIYRALLCS
                     EARRVIQMEGYRAIRTLKCGGKFHHFTAKRRGAKFAVNLKVRATTGQVIVAGRNR"
     gene            complement(2842..3045)
                     /locus_tag="PKV23_04900"
     CDS             complement(2842..3045)
                     /locus_tag="PKV23_04900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX84807.1"
                     /translation="MTPDDFDDEPRASLSLLQVYRLIALEQIEMDRRVAERRSANVIP
                     LRTPAGRNLEIRLKPDLRRRLAG"
BASE COUNT          585 a          963 c          927 g          607 t
ORIGIN      
        1 gcgccagcaa ggcaaggccg agattgttat gggcttcgga ataatcgggc ttcagccgca
       61 gtgccgtgcc gaagctggcg atggcctcct ccaggcggcc ccggtccttc agtgccgcac
      121 cccggttgtt gaaagcctcg gcaaggtcgg gcttgtactg cagggctttg tcatagcttg
      181 cgatggcttc atccagaagg ccctggcgat gcaaggcggt accaaggctg ctgtgcgcct
      241 cggcgaaatc gggcttgagc tgcaccgcct tgatgaagct tgcagccgcc tcctgcaggc
      301 ggcccaggtc tttcagcgca acgccaagat tgttgtgggc cggggcgaag ccgggcctga
      361 gctgcaccgc cttgccgaag cttgcagcgg cgccatccag gttgcgcaat cccatctggg
      421 ccgcacccag gatgttgaag acaatggcgg cgttcggata ttccgcagcc atcgctgttg
      481 cctgctggac ggcctccttc aggcgccgct ggttcatgag cgcgacaagg ccatcgagcc
      541 ggccctgcgg cggctctgcc ggcgaggctt tggacgtttt taccttgccc tgtttcctgg
      601 cattttggag gacctgttca aaataattgg ccatggcgcg gggccgatta ttccgccgcg
      661 accatctgcg gctggtagtt cgaaaggctt tcgctgacat ggcgcgccag ggcatcgatc
      721 gccttgttgg attttccggg cttgcgcacc agtccgatat cgaaacttcc aagcggcggg
      781 aagccatctt tctcggtgag aacccgcatg cccggtcgca cgcagagttc cggcatggcg
      841 ccaaccgcga ggccttgcat aacagccgca ttcaccgcgt ttgaattggg cgtggaatag
      901 gccacgcggt atttcctctg ctgcttgtca agcgccttga gcaccttgct gcgccactcg
      961 cagcccgcat gcgagaccgc cagcggcagc acctccgcca tgtgggcggc gtggcgcatg
     1021 gaggtgaccc acaccagctg ctcgcgccgc accggttcgt cggtgatgat gtcacagccg
     1081 tgggtgacca gggcgagatc aatctcgctg cgcttcacgc gggcgaaaag ttcgccgctg
     1141 cccacgcagt ccacatcgac ggtgatcagc ggatgggtgc gggcaaaacg cgccaggatc
     1201 tccggcagga aacgttcggc gtagtcatcg ggcgtgccga agcggatggt gccggtgagc
     1261 tccggcttgg tgaaggcgga taccacttcg tcattgaacg atacgatgcg ccgggcgtaa
     1321 tccaccaggc gctcgccatc gggggtgaaa cggctcatcc ggccatcctt ggcaaagagc
     1381 gccaccccca gaagctcttc cagccgcttc atctgcatgg atatggccga ctgggtttta
     1441 tgcacctcct cggccgcccg ggtgaagctg ccggtgtcgg caatggccag gaaggtcttc
     1501 aacaaatcga catcaagatt ggcgatcatg gcataccccg tatcatcaac gttcgtgatg
     1561 agtatcataa aatacattcg ttagactaat caatagaaat cttcatagtg ggttcatcac
     1621 ggatgtgatt ttagagccgc tttcgggtgg ttttgcccgg gaaaaatgga ttttctggaa
     1681 ttttggagga ctgtgccatg agagactatg ctttgaacca agccctgtcc cggggtgaat
     1741 atgggatcgt ttacttcctg cgccgcgtca cgcggaactg gaagtccaag cggcggatcg
     1801 cgagccttgg caacttcgac gactacatgc tgcgcgatat cggggtcacc cgcgacgaag
     1861 tgcaatgggc cgccggcctg ccgctgaccg ttaacgccgc cgtggccctg gaggaacggg
     1921 cactccgccg ccgcaagtcg ggccgtctgg aatgatcgcc aggcgcggac tgcgcacccc
     1981 cgttcccccg cttcatcctt aaggccctgc tccagaaacg tggcggggcc ttttctctgg
     2041 gcagccgcgg cgcccgcatg gaacctttcg acaatagccg ccgttagaac ccggtctcat
     2101 ccagcaaagg attccatcat gatgattgac cccaagggtt cacagccgga agcgcttgcc
     2161 aagtttgccg gggtggcacg ccatgccgac ggcaagccgc tgcgcgaagg tgttccgcac
     2221 cgccatcagg gcgccaaaaa tgccatcaac aaattacctg atcgcaccgc gaaaaatggt
     2281 aagggccgtg gcggtgcccg ctcccgataa accgcgccgg cccctggagc gtcataaggt
     2341 gttcatgcag actgtctttg cggccctcgc aatcctgatc gcgacggcac cggcccatgc
     2401 cgcatccttg ttcgacacgc cgggatcgaa tcccgcggcg aaccgctgcg acctgttcga
     2461 tcccaactgc aagccgcggc aattgttcgt caggccgccg gagccggtca ccaagaacaa
     2521 tcccttgggc gctccggcgc cgaaattgat tctgccgcgc gaccagaatg accgttctgc
     2581 aaagccctcg ccgctgctcc acgtgccggg tcagcccagc cgccggatat accgcgccct
     2641 gctgtgcagc gaggcccggc gtgtcattca gatggaaggc taccgcgcta tccgcaccct
     2701 caaatgcggc ggcaagtttc atcacttcac cgccaagcgg cggggcgcga aattcgcggt
     2761 gaacctgaag gtccgggcaa cgacgggaca ggtcatcgtg gctggccgca accgctgaac
     2821 tcgatccgga gttctggacc tctaacccgc aagccggcgg cgaagatcag gcttcagccg
     2881 gatttcaaga tttctgccgg ccggggttct caaaggaatg acgtttgcag aacgtctttc
     2941 cgccacccgc cggtccatct cgatttgctc cagtgcaatg agccggtaca cctgcaagag
     3001 cgaaaggctc gcccgcggtt catcatcaaa gtcatcaggg gtcatgcgca tctctctcac
     3061 ttatgcaata tgtttgcatc ac
//
LOCUS       DAORVN010000191         3081 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_28053_length_3081_cov_2.340383, whole genome shotgun sequence.
ACCESSION   DAORVN010000191 DAORVN010000000
VERSION     DAORVN010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3081
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28053_length_3081_cov_2.340383"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..352
                     /locus_tag="PKV23_04905"
     CDS             <1..352
                     /locus_tag="PKV23_04905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="long-chain-acyl-CoA synthetase"
                     /protein_id="HQX84808.1"
                     /translation="AGVETVTVYGVSVPGQEGRAGMATLVMQPGQRFDPVACHQLAAK
                     ALPSYAIPLFIRVKADADITATFKLRKVDLQKLAYAPSADDELYVADAAAGRYVALTE
                     ESLSRLGIPPSPPI"
     gene            367..852
                     /locus_tag="PKV23_04910"
     CDS             367..852
                     /locus_tag="PKV23_04910"
                     /inference="COORDINATES: protein motif:HMM:NF024966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HQX84809.1"
                     /translation="MSTEDQERRLCAIEDRFAILDLEAEYAHGWDLGTPRQWAEVFTE
                     DGVFEMLAIGQTPHVRIVGHAALEAFCAQIRQDWSGLHYMHPPRLRIDGDRAESVIFF
                     EFRHVMRTPTHIRQGVTAGHYRTRYMRTSAGWRMRERIEQAVGEETAHFYPASLASSP
                     T"
     gene            complement(908..1702)
                     /locus_tag="PKV23_04915"
     CDS             complement(908..1702)
                     /locus_tag="PKV23_04915"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQX84810.1"
                     /translation="MSAHADMNMQPALATPATGVIITGGASGLGRASAHALAAVGRPV
                     AIWDINASAALIVAEEVRSQYAVAAIGIGLDISVPAAYPDAIEKSRRTLTSIGGLVHA
                     AGIVDTGSLEGVTLENWDQGINIHLRPVALLVQALHADLKANPGSAVVAFASINATLG
                     NRMNPIYSAAKGGVLSLVRSLADRLGDDGIRINSISPGQILTPMLQPAVDALPPGSFE
                     KRILLGRMGAPEEIGRVVRFLLSNEASYITAAEIVVDGGNVSSQRG"
     gene            complement(1686..2825)
                     /locus_tag="PKV23_04920"
     CDS             complement(1686..2825)
                     /locus_tag="PKV23_04920"
                     /inference="COORDINATES: protein motif:HMM:NF018452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1214 domain-containing protein"
                     /protein_id="HQX84811.1"
                     /translation="MSPTKQTSVQAWEAFCDQLKQAGAVLARDGTPDDELTQAEGLRK
                     LVRMIRMGFEASLEYGNTDFPEVYQLVTPTTVGEGENADAHYHQAMIDGSKTYRISGR
                     RGDAPFIELTVYAGKIGLDSGSAQVGAITEADLKVGPDGSYELMLSPDPQPGNWIRTT
                     PEATVLYIRQYAHDWRKARDTTFKIERVGATGYRPSLTVAEVSQAMNRTATYVARSSN
                     IWAAIVDQRRAAPPNRIFNFEEEQKDSGEEAPEMPIGHRFSSGYFRLAEDEALVLSFK
                     PADVPYWGMAMTNYWFEPFTYTEHRSHYNNRTVHYEADGSVKIVIAAKDPGVPNWMDT
                     MGHLEGPVIFRWSRTRLPVPDIQAEVVKLANLNRNEEKIQDERTR"
BASE COUNT          546 a          986 c          963 g          586 t
ORIGIN      
        1 ggcgggcgtg gaaacagtca cggtgtacgg ggtgtccgtc cccggccagg aagggcgcgc
       61 gggcatggcc acactggtga tgcagcccgg acagcgtttc gatccggtgg cgtgtcatca
      121 actcgctgcc aaggcgctgc cgagctacgc gattccgctc ttcatccgcg tcaaggccga
      181 tgccgacatc accgccactt tcaagctacg caaggtggat ctgcagaaat tggcttacgc
      241 gcccagcgct gatgacgaac tgtatgttgc tgatgccgct gcaggccgat acgtggcgct
      301 gaccgaagag agcctgtccc ggctcggcat tccgccttcg ccgccaatct aaaaggagca
      361 agacctatgt cgacagaaga tcaggaacgc aggctttgcg ctatcgagga tcgcttcgcg
      421 atcctcgacc tggaggccga atacgcccat ggctgggatc tgggcacgcc gcggcaatgg
      481 gccgaggtgt ttaccgagga cggtgtcttc gaaatgctcg ccatcggcca gacgccgcac
      541 gtgcggattg tggggcacgc ggcgctggaa gctttctgtg cgcagatccg acaggactgg
      601 tcgggcctgc attacatgca tcccccaaga ctcagaatcg atggcgatcg ggccgaatcg
      661 gtgatctttt tcgagttccg ccacgtcatg cgaacgccaa cgcacatccg ccagggtgtc
      721 accgcgggcc actatcgcac ccgctacatg cggaccagcg cgggttggcg catgcgggaa
      781 cgaatcgaac aggccgtagg cgaggagacg gctcacttct atccagccag cctcgcctca
      841 tcgccaacct gagcatagcc ctgagcgccg aactcagcgc cgaactcagc gcagcacggt
      901 tgccgcctca gccgcgctgc gaggaaacgt tccccccgtc caccacgatc tcggcggcgg
      961 tgatgtagct ggcttcgttg gacagcagaa agcgcaccac gcgaccgatc tcctctggcg
     1021 cccccatgcg cccgagcagg atgcgctttt cgaagcttcc cggaggcagc gcatccaccg
     1081 ccggctgcag catcggggtg aggatctggc ctggagaaat ggaattgatg cggatgccat
     1141 catcgcccag gcggtcggcc agcgagcgca ccagggacag caccccgccc ttggcggcgg
     1201 aatagatcgg gttcatgcgg ttgcccaggg tggcattgat ggacgcgaag gccaccacgg
     1261 ctgagccggg gttggccttc aggtctgcat gcagcgcctg caccaatagc gccaccgggc
     1321 gcaaatggat gttgatgccc tggtcccagt tctccagcgt caccccttcc agggacccgg
     1381 tgtcaacaat cccggccgca tgcaccaggc cgccgatact cgtcagtgtc cgacgggatt
     1441 tctcgatggc atctggataa gccgccggaa cactgatgtc cagaccaatg ccgatggcag
     1501 ccactgcgta ctgactgcgg acctcctcgg ccacaatcag ggcagcgctt gcgttgatat
     1561 cccagatcgc caccgggcgg ccgacggcag ccagcgcatg agcgctggcc cggcccagtc
     1621 cggaagcgcc accggtgatg atgacgccgg tggccggggt cgcgagggcg ggctgcatgt
     1681 tcatgtcagc gtgtgcgctc atcctgaatc ttttcttcgt ttctatttaa gttggcaagt
     1741 ttgaccacct cggcctggat atccggcacc ggcaggcggg tgcgcgacca gcggaagatc
     1801 actggccctt ccaggtggcc catggtgtcc atccagttgg gcacgcccgg atccttcgcg
     1861 gcgatgacga tcttcacact gccatccgcc tcgtaatgga cagtacggtt gttgtagtgg
     1921 gaacgatgct cggtgtaggt gaatggctcg aaccagtaat tggtcatggc catgccccag
     1981 tagggcacgt cggccggctt gaagctcagc accagggcct catcctcggc caggcggaaa
     2041 tagcccgacg agaaacgatg gccgatgggc atctccggcg cctcctcgcc gctgtccttc
     2101 tgttcctctt caaagttgaa gatccggttg ggcggcgcgg cgcggcgttg atcgacgatg
     2161 gccgcccaga tattggacga gcgcgccaca taggttgcgg tgcgattcat ggcctggctg
     2221 acctccgcca ccgtcagcga ggggcgatag cctgtggcgc caacgcgctc gatcttgaag
     2281 gtggtgtccc gcgccttgcg ccagtcatgg gcgtactgac gaatgtagag caccgtggcc
     2341 tccggcgtgg ttcgaatcca gttgcccggc tggggatcgg gcgacagcat caactcgtag
     2401 ctgccatcgg gccccacttt caggtccgcc tcggtgatcg cgccaacttg cgcgctgccg
     2461 ctgtccagcc cgatcttgcc ggcatagacc gtgagctcga tgaagggcgc atcgccccgc
     2521 cgaccactga tccggtaggt cttgctgccg tcgatcatgg cctgatgata gtgggcgtcc
     2581 gcattctcgc cttcccccac cgtcgtcggc gtcaccagtt gataaacctc cgggaagtcg
     2641 gtgttgccat attccaggct ggcctcgaag cccatgcgaa tcatccgaac cagcttgcgc
     2701 aggccctcgg cctgggtcag ctcatcgtcc ggcgtcccgt ctcgggccag caccgcgcca
     2761 gcctgtttca attgatcgca aaacgcctcc catgcctgca cactcgtctg tttggtcggg
     2821 ctcattgata ctcctcgcat aggctcaggg gcgcctgccg gcatagtgct tccggcatgg
     2881 caggatgtga agaaggccat ccccaggatg gccaggcatc gtctcattgc ccctcgctgg
     2941 cgacgtggaa gtagtcctgg tagaaacggt agcgctcccg ctcctgcgcc tgattcagac
     3001 cgaactgctc cagggtgtag cggtgggtgc cgtggcgttc cttcggattg ttggcgatga
     3061 agtcgcgcat tttctgctcg g
//
LOCUS       DAORVN010000192         3079 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_28081_length_3079_cov_3.143519, whole genome shotgun sequence.
ACCESSION   DAORVN010000192 DAORVN010000000
VERSION     DAORVN010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3079
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28081_length_3079_cov_3.143519"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="PKV23_04925"
     CDS             <1..229
                     /locus_tag="PKV23_04925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX84812.1"
                     /translation="GRYKVIDPTFRGHVWMDRETIDAEASGIFLVPKAKAPEPWAKVS
                     PELFRVIATVMPVAFWNSLTMLWHHSSCTEQ"
     gene            429..1214
                     /locus_tag="PKV23_04930"
     CDS             429..1214
                     /locus_tag="PKV23_04930"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HQX84813.1"
                     /translation="MDEGELDRRFHAGLGLVREVGQEACRQYRRRDSLAVETKGLQDL
                     VSETDRACEDEIVSALNRMFPGDAFLGEERGLQNTGGDATWVIDPIDGTANFLRGIPL
                     WCVSLGLVYRKVPVIGLIYNPVTDELYAAKRGRGATLNNSRIKVGTVTRLSEARMGLG
                     FSYRRPVAPHVQAISALLEAHCEYSRLGSGALGMAMTADGRLDGYWEAHINIWDVAAG
                     LCIVGEAGGWTNDFMSGDVFRLGNPILATNPALAAPLQALLPL"
     gene            complement(1193..2047)
                     /locus_tag="PKV23_04935"
     CDS             complement(1193..2047)
                     /locus_tag="PKV23_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010910970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HQX84814.1"
                     /translation="MNQVDLDDVRRSYARDMARVSASEDPRLEQAFYSVHREDFLPPG
                     PWKLLVEGRYVDTPNSDPVHVYRNSAIALDARKGINNGEPHLHAMWIGAVAPKPGEDI
                     TQIGLGMGYYSAILSLLVQPGGRLTGFEIEGELAEIARRNLADFKNVSVVAGDAASRE
                     LPPSDLIYVSAAVAAPPESWLTALRPGGRLIFPWRPTIEVALAMLVRRVPDGFEARPL
                     MTSWFIPCAGAASPRGSALPPTPSTAWRIRSLHLSRDRAPDKSAVAVYQHVWFSSAKL
                     RGYNGSKA"
     gene            2180..2446
                     /locus_tag="PKV23_04940"
     CDS             2180..2446
                     /locus_tag="PKV23_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012564322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HQX84815.1"
                     /translation="MTTTSSRFFDELAKLMTNATGAAQGLRKEVDAMVQSQVERVLNN
                     LELVKREELDVVRDMARKAREENEALAKRLAELESKAISDVSIP"
     gene            2562..3062
                     /locus_tag="PKV23_04945"
     CDS             2562..3062
                     /locus_tag="PKV23_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018183703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HQX84816.1"
                     /translation="MGSLQFYENNTTNPLDLIERLAERRDWSVDRTNDCEVTMVMGGG
                     WSDLYVSLNWRDDLESLLVACTYDIKVPPARKDEVGRLLSLVNGQLFHGHFDMWHADG
                     SIVFRNNLVLAGGAVANDAQCEALIRLGIESCERYYPAVQYVCWAGQSADAALESSLL
                     ETMGEA"
BASE COUNT          615 a          926 c          924 g          614 t
ORIGIN      
        1 cgggcgttac aaggtgatcg accccacgtt tcgcggccat gtctggatgg accgggagac
       61 gattgacgcc gaagccagcg ggatatttct cgtgccaaag gctaaagcgc cggagccatg
      121 ggcaaaggtt tcgccggagc ttttccgcgt catcgccacg gttatgccgg tggccttctg
      181 gaattcgttg accatgctct ggcaccattc ttcctgcacc gagcaataaa ccgtgagatt
      241 gccttcggcc tgtgcgctcg agccgaacgc aagcgctgca aatgccagaa cagccgcgga
      301 attaaagaat tttcgggtga ttagggtcat gctttcctcc cagttcagac ttttgtttat
      361 gacagttttg tgacaacatg gcagaagaaa ttgctgttgg ccagcatgga ttttccggga
      421 gattgaggat ggacgaaggt gaacttgacc ggcgctttca cgctggcctg gggttggtgc
      481 gtgaagtggg ccaggaggcc tgccgtcagt accgccgccg tgactcgctg gcggttgaaa
      541 ccaagggcct tcaggacctg gtgagcgaaa ctgatcgcgc ctgcgaggat gaaatcgttt
      601 cggccctcaa ccggatgttt cccggcgacg ctttcctcgg tgaggagcgc gggctgcaga
      661 ataccggcgg tgacgccacc tgggtcatcg atcccataga cggcaccgcg aacttcctcc
      721 gtggcattcc gctctggtgc gtctcgctcg ggctggtcta ccggaaggtc ccggtcatcg
      781 gcttgatcta caatccggtg accgatgaac tctatgccgc caaacgcggg cgcggagcca
      841 ccctcaacaa ttccaggata aaggtcggca ccgtcaccag gctgagcgaa gcgcgcatgg
      901 gcttgggctt cagttaccgt cgcccggtag caccccatgt gcaggcaatt tctgcgctgc
      961 ttgaggccca ttgcgaatac agccgcctgg gctccggcgc cctgggcatg gccatgaccg
     1021 ccgatggccg cttggacggc tactgggaag cgcacatcaa catttgggac gtggcagccg
     1081 ggctttgcat cgtgggcgaa gcaggcggct ggaccaatga cttcatgtcc ggcgatgtct
     1141 tccgcctggg caacccgatt cttgccacca atccagcact ggccgccccg cttcaggcct
     1201 tgctgccatt gtaaccgcgc agcttcgcgc tggaaaacca gacatgttgg tagaccgcga
     1261 cagcgctttt atccggcgcg cggtctcggc tgagatgaag cgagcgaatg cgccacgccg
     1321 ttgaaggcgt gggtggcagt gcgctgcccc tgggacttgc tgccccggcg caggggatga
     1381 accaggatgt catcaggggc cgcgcctcga aaccgtccgg cacccgcctc accagcatgg
     1441 cgagggccac ctctattgtc ggccgccagg gaaagatcaa acgaccgccg gggcggagtg
     1501 ctgtaagcca actctcgggc ggagcggcga cggcggcact cacatagatg aggtccgaag
     1561 gtggcagctc ccttgaagca gcatcgcctg cgaccaccga gacgttcttg aaatccgcaa
     1621 gattgcgccg cgcaatctcc gccagttcac cttcgatctc aaatccggtc agccgcccgc
     1681 cgggctgcac cagaagtgac aggatggcgg aatagtaacc catccccagg ccgatttgcg
     1741 tgatgtcttc cccgggcttt ggcgccaccg ccccgatcca catggcgtgc agatgcggtt
     1801 cgccattatt gatgcctttc ctggcatcaa gggcaatggc ggaattccgg tagacgtgaa
     1861 ccggatccga attcggcgtg tcgacatacc gcccctccac aagcaatttc cacggccccg
     1921 gcggaagaaa atcctcgcgg tgcacactgt aaaatgcctg ttcaaggcga gggtcttcag
     1981 acgcactcac ccgcgccata tccctggcgt aggaccggcg gacgtcgtcc agatcaacct
     2041 ggttcaattc ggcctcaatt cgcaaatttc ctttcagcct gcctctatgc aagttaacat
     2101 taagtgggcc cgtggcacta cggcccttga aaaaaccttg ctgaatccat agttacacag
     2161 cacgaagagg aatgctgcca tgaccaccac atcatcccgg ttttttgacg agcttgccaa
     2221 gctgatgacc aatgccacgg gggccgccca gggcctgcgc aaggaagtgg acgccatggt
     2281 ccagtcccag gtcgagcggg ttttgaacaa cctcgaactg gtcaaacgcg aggaactgga
     2341 cgttgtccgc gatatggccc gcaaggcccg cgaggaaaac gaagccctgg ccaagcgcct
     2401 ggctgaactg gaatccaagg ctatctcgga cgtctccatt ccataattta tgtggaggaa
     2461 tcagcggcaa ttgcccgttg actcttgtgg cgactcaatc gcacgggcat tttcgctgca
     2521 atgcccgggt gtgattcgtt gggcacgtga ggacaaggcc catgggttca ctgcagtttt
     2581 acgagaacaa tacaaccaac ccgctcgatc tcatcgagcg cctcgccgag cgccgcgact
     2641 ggtccgttga ccgcaccaat gattgcgaag tgaccatggt gatgggtggc ggctggagcg
     2701 atctttatgt ctcgctgaac tggcgcgatg acttggaatc cctgcttgtg gcctgcacct
     2761 atgacatcaa ggtgccgccg gcgcgcaagg acgaggtggg ccggctgctg tctttggtca
     2821 acgggcaatt gttccatggc catttcgaca tgtggcacgc cgacggcagc atcgtcttcc
     2881 gcaacaatct ggttctggcg ggcggcgccg tggccaatga cgcccagtgc gaagccctca
     2941 tccgtctcgg gatcgaatcc tgcgaacgct attaccccgc tgtgcagtat gtctgctggg
     3001 cgggccagag cgccgatgcc gccctagaaa gcagcctgct cgaaaccatg ggtgaggcat
     3061 gagccttgcg ctcagcgga
//
LOCUS       DAORVN010000193         3078 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_28094_length_3078_cov_2.715184, whole genome shotgun sequence.
ACCESSION   DAORVN010000193 DAORVN010000000
VERSION     DAORVN010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3078
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28094_length_3078_cov_2.715184"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /locus_tag="PKV23_04950"
     CDS             complement(<1..500)
                     /locus_tag="PKV23_04950"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF014873.2"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the beta chain is a
                     regulatory subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HQX84817.1"
                     /translation="MHRAIYVCLNGERFTFEVHRHLDEHRVRAIALHRTSGLKRQMPV
                     FDAGGGLQIPVTPDCLGRLLDIFGRPLDGGPALVPQAMRPIVAAPVPLSEVTGSGDVL
                     LTGIKVIDLLCPFIRGGKTGLFGGAGVGKTVLIMEFMNAIVRLHQGVSVFAGVGERIR
                     EGHELW"
     gene            complement(638..1006)
                     /locus_tag="PKV23_04955"
     CDS             complement(638..1006)
                     /locus_tag="PKV23_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011472391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097159 - organic cyclic compound binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b5-like heme/steroid binding
                     domain-containing protein"
                     /protein_id="HQX84818.1"
                     /translation="MTRKLYIVSTTAFWLAVLGLGMTSALWNPAEQGDVQAQGKTVTL
                     AELAKHAAPGNCWMAIRGSVYDLSAYLPAHPSPPEIVLPWCGKEATEAYNTKTKGRPH
                     APYADELLAKYRIGTLVKAP"
     gene            complement(1003..2256)
                     /locus_tag="PKV23_04960"
     CDS             complement(1003..2256)
                     /locus_tag="PKV23_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011663773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HQX84819.1"
                     /translation="MSRLHLLSLPILLLLFVGLPVFWAFPKALPFWRSGGIVLGWAGA
                     GLLLFNLLLMVREVRLASSLGGLERITVWHHQTGMVAYLLLLLHPLALAAAGWGESPE
                     LAWKVISPISESWPVWLGWGGLIFLMLGLATTFMNHLAYGTWRWLHGLLGLGVLIGFA
                     HVLQLGISAGAVFALFAALLLLLWRLLRVDLGTAAKPYIVTSVRAVAQSMVEVSMKPL
                     AMPIAVVPGQFVLAAFFKGPGYQGCGEFHPFTVSGLGQDHEFRIGVKALGDCTRHMQR
                     LEAGVSARIHGPFGEFLKDLPTRPLLWVAGGIGITPFLGFLRQWPVISPTTLIYLYRG
                     EQDAAYLDELTALSAGCAELSLQAEATGSGLPDLNRLLPEAAALANHECYLCGPPGLI
                     EAISRILLERNVPVDRIHFEHFDFR"
     gene            2303..2545
                     /locus_tag="PKV23_04965"
     CDS             2303..2545
                     /locus_tag="PKV23_04965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX84820.1"
                     /translation="MLADIATTRRQGHALLGGPPRHQAMVFLLVGTTFIEGFEATDTR
                     LMFKALLRAHELPTPGILFAGTPRAASLDSHCCREQ"
     gene            complement(2619..3014)
                     /locus_tag="PKV23_04970"
     CDS             complement(2619..3014)
                     /locus_tag="PKV23_04970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX84821.1"
                     /translation="MDLIKQILASFEGTLPNDSKTAAAIARGAAPEEISACATEEGLH
                     ALAGALFEACEERGEDAHLDGSARVTGALAERLKEFRQGLPPACETARLIDAGARLEE
                     ISEAAEKEGLNALASMLFEAEQEQGKDTA"
BASE COUNT          699 a          963 c          878 g          538 t
ORIGIN      
        1 tgccagagtt catggccttc acggatgcgc tcgccaacgc cggcaaagac cgaaacgccc
       61 tggtgcaggc gaacgatggc attcatgaac tccatgatca gcacggtctt gccgacgccg
      121 gcgccaccga acaagccggt cttgccgccc cggatgaacg ggcacaacaa atcgatgacc
      181 ttgattccag taagcagcac atcgccgctg ccagtgactt cagacaaagg cacgggggcc
      241 gccacaatgg gacgcatggc ttgcggaacg agcgccgggc caccatcgag cggacgaccg
      301 aagatatcca gcaaacgccc caggcaatcc ggggtaaccg gtatctgcaa accgccgcca
      361 gcatcgaaaa ccggcatttg ccgcttcaga ccgctggttc gatgcagggc aatagcccgt
      421 acccgatgct catcaagatg tcgatgcacc tcgaaggtga agcgttcccc gttcaggcaa
      481 acgtaaattg cccggtgcaa cgggggcaag cgggaacagc ggacatcgac gacggggcct
      541 tgtatttcct caatcacacc caagggtggg ccatcgggta ccgggcaacc cggcaccagt
      601 tcctgatagc aactcgatgg tccgcaggca ttcatgatca aggcgctttt accaatgtcc
      661 cgatacgata ttttgccagc agttcgtcgg cataaggtgc gtgcggccga cccttggttt
      721 tggtgttgta ggcttccgtc gcctccttgc cgcaccaggg aaggacaatt tccgggggag
      781 atggatgggc tggcagatag gcgctgagat catagaccga accacgaatc gccatccagc
      841 aatttcccgg cgcggcatgc ttggccagtt ctgccagcgt gacggttttc ccctgggctt
      901 ggacgtcgcc ttgttcggct ggattccaca gcgcagaagt catgcccaga cccaatacag
      961 ccagccagaa ggcggtagtc gaaacaatat agagtttgcg tgtcatcgga agtcgaagtg
     1021 ctcaaagtga attcggtcaa cggggacgtt acgctcaagc agtatccggg aaatggcctc
     1081 aatcaagccc ggcggaccgc agaggtaaca ctcgtggttt gccaacgcag cagcttctgg
     1141 caacaagcgg ttcagatccg gcaggccgct tcctgtcgcc tcggcttgca aggaaagctc
     1201 ggcgcaaccg gcagacaagg ccgtcaattc gtcgagatag gcggcatcct gctcgccccg
     1261 atacaaataa atcaaggtcg tcggactgat gactggccac tgccgcagaa agccaagaaa
     1321 cggggtgatg ccgatgccgc cggccaccca caacagcgga cgcgttggca aatctttcag
     1381 gaactcgcca aagggaccat gtatccgggc ggaaacccct gcttccaggc gctgcatgtg
     1441 ccgagtgcag tcgccgaggg ccttgacccc gatgcgaaac tcgtggtcct gcccaagacc
     1501 gctgacggtg aatgggtgga actcaccgca gccttgatag ccaggcccct tgaagaaggc
     1561 cgccaggaca aattgccccg gcacaacagc aatcggcatg gccaagggtt tcattgaaac
     1621 ctccaccatc gactgcgcca ccgcccgaac cgaagtcacg atatagggct tggcagccgt
     1681 tccgagatcg acccggagca gccgccaaag caaaagcagg agggccgcga acagcgcaaa
     1741 caccgcacca gcgctgatcc cgagctgcaa aacgtgggca aaaccgatca ggacaccgag
     1801 cccaagcaag ccatgcaacc aacgccaggt gccataggca agatggttca tgaacgtggt
     1861 ggccaacccc agcatcagga aaatcagccc gccccagccc agccagaccg gccaactctc
     1921 tgaaatcggc gagatgacct tccaggcaag ttctggcgat tcgccccacc ctgctgccgc
     1981 cagggccagc gggtgcaaca aaagcagcag gtaagccacc atgccggttt gatggtgcca
     2041 aacggtaatg cgctccagtc cccccagact gctggcaagg cgaacctccc gcaccatcag
     2101 cagcaaattg aacaaaagca gcccagcccc cgcccagccg agaactatcc cgccagaccg
     2161 ccagaacggc agtgctttgg gaaacgccca aaaaacgggc agccccacga aaagcaggag
     2221 cagtatcggc aaggacagca ggtgcaggcg agacatttca ggattccgcc atgacttcgt
     2281 tgctttattt cggcgggatc agatgctcgc agatatcgcg accacccgac ggcaaggcca
     2341 tgcccttctc ggtggcccgc cgcgccatca agcgatggtg ttcctgctgg taggcacgac
     2401 attcatcgag ggttttgaag ccacggacac gcgcttgatg ttcaaggcgc tcctccgggc
     2461 tcatgaattg ccaaccccgg gtattctctt cgctggcacg ccaagggccg cgagcctcga
     2521 cagtcattgc tgccgtgagc agtagcgccc cggatattgc catcaacagc cgccgcgatc
     2581 tcattggagc ctccctgaat gctggtgtta tggggacatc aggcggtgtc tttgccctgt
     2641 tcctgctcgg cctcgaaaag catgctggcc aaggcgttca aaccttcttt ctcggccgcc
     2701 tcggagattt cttcgagcct ggcaccggca tcgatcagcc gggctgtttc acaggccggc
     2761 ggcaggcctt ggcggaattc cttaagtcgc tcggcgagcg ccccggtaac ccgcgccgaa
     2821 ccatcgaggt gggcgtcttc acctctttcc tcgcaggctt caaacaaggc accggccaag
     2881 gcatgcaaac cttcctcggt cgcgcaggcg gaaatctctt ccggtgcagc cccccgggcg
     2941 atggcggcgg ctgtcttgct gtcattgggc agcgtgcctt cgaacgatgc cagtatttgc
     3001 ttgatcaggt ccatgtcagt ctccttgtta tgtatttggc aagagcgata ttgcgctcaa
     3061 taacccgagc agggaata
//
LOCUS       DAORVN010000194         3067 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_28265_length_3067_cov_3.250332, whole genome shotgun sequence.
ACCESSION   DAORVN010000194 DAORVN010000000
VERSION     DAORVN010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3067
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28265_length_3067_cov_3.250332"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            151..1083
                     /locus_tag="PKV23_04975"
     CDS             151..1083
                     /locus_tag="PKV23_04975"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006202834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline/ethanolamine kinase family protein"
                     /protein_id="HQX84822.1"
                     /translation="MDDQGIGLAREALKQIQLFWCLDDTDIEVRRLGGLTNLVFFVSL
                     NGEQYVLRIPGKGTEEYINRKNEAQAAREAAKAGVSPDVVHFDDKTGVMVTRFVDHGV
                     TMSPEHFKTRDGAPKRAGEAFKKLHSSGARFNFRFELFAMIDDYLKILATKDISLPDG
                     YAEVVKEAESVRQALDAHPLPLVACHCDPLCENFLDTGKRMWIVDWEYSGMNDPLWDL
                     GDLAVEGQFSAAQEEEMIAAYFGGEPTAAERGRFVIYKAMCDLLWTLWGLIQHANKNP
                     VDDFWAYSINRFERCKKLMATAEFGKHVAAVKKG"
     gene            complement(1090..2238)
                     /locus_tag="PKV23_04980"
     CDS             complement(1090..2238)
                     /locus_tag="PKV23_04980"
                     /inference="COORDINATES: protein motif:HMM:NF019379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1615 family protein"
                     /protein_id="HQX84823.1"
                     /translation="MIRLFICFLVALWSACSAFSAEEPMALSTPAVARLINAAEKSVK
                     DSRGWADDLLDVLKLHNLPASKEDICAAIAIIDQESSFVADPAVAGLGKISEAALRAK
                     MDKVPVLGRVALHFLEVTPSPADNYLARIRAARTERDLDLVYRAMVADAGKQTGLGLV
                     INSGLLNKQIDGRNEIDTIGSMQVSVDFALEVAKRRRWLPMALDDVYALRDELYTRHG
                     GMYYGVLLLLGYETGYSRKLYRFADFNAGRYSSRNAALQSQIAELSGTALAADGDLLL
                     YDKSGKVSGGVSKTEMALRAVLKNSSFELTDEDIRKDLLREKDNDFVQTLTYSALRAT
                     YEQSTGRRPAFAMVPNIALSSPKIRSKMTTRIFAETVDRRYQSCMARK"
     gene            complement(2316..2930)
                     /gene="gfa"
                     /locus_tag="PKV23_04985"
     CDS             complement(2316..2930)
                     /gene="gfa"
                     /locus_tag="PKV23_04985"
                     /EC_number="4.4.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019171709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051907 - S-(hydroxymethyl)glutathione
                     synthase activity [Evidence IEA];
                     GO_process: GO:0046292 - formaldehyde metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione synthase"
                     /protein_id="HQX84824.1"
                     /translation="MAKKAKTKAAAKKSKAISLHPLLDKGVLGKAKAKFAGGTLTCKC
                     TTDPVIVSVGAQTAHNHACGCTKCWKPSGAIFSVVAVVGRDNVKVTANENKLKIVDAN
                     ALIQRHACTGCGVHMYGRVERTTHGFYGLDFIHTELSKEKGWSPPEFAAFVSSVIESG
                     TKPEDMPAIRKRLKQVGLQPYDCLSPALMDYLAAHAAKASGVLK"
BASE COUNT          630 a          801 c          979 g          657 t
ORIGIN      
        1 gcccagcgtg ggccacgctg cctttacgac gcgaagatgg aaaggctgaa ggcgtgagcc
       61 gcaggaacat ttttgatctt cttaaaatga atcagaagac ctatacaagc aattgtaatt
      121 tatagggttt accgaaaggc gaaaaggtcg atggatgatc agggcatagg cctggcgcgg
      181 gaggcgctga agcagatcca gctcttctgg tgccttgatg ataccgacat tgaggtgcgg
      241 cgcctgggcg ggctgaccaa cctggtgttt ttcgtgagcc tcaatggcga gcagtatgtg
      301 ctgcgcattc cgggcaaggg cacggaggaa tacatcaacc gcaagaacga ggcccaggcg
      361 gcacgagagg cggcgaaggc cggggtgagc ccggacgtgg ttcatttcga tgacaagacg
      421 ggtgtgatgg tgacccgctt tgtggaccac ggcgttacca tgtcgccgga gcatttcaaa
      481 acccgtgacg gcgcgccgaa gcgggcgggc gaggcgttca aaaaacttca ttcctccggg
      541 gccaggttca atttccgctt tgaacttttt gcgatgatcg atgactatct gaaaatcctg
      601 gcgacgaagg atatttccct gccggatggt tatgcggagg tggtgaaaga ggccgaatcg
      661 gtgcggcagg cgctcgatgc ccatccgctg ccgctggtgg cctgccattg cgatccgctg
      721 tgcgagaatt ttctcgatac gggcaagcgc atgtggattg tcgactggga atattcgggg
      781 atgaatgatc cgctgtggga tctgggcgac cttgcggttg aggggcaatt cagcgcggcg
      841 caggaagaag agatgattgc cgcctatttc gggggcgaac ccaccgcagc ggagcgcggg
      901 cgcttcgtga tctacaaggc gatgtgtgat ctgctgtgga cgctgtgggg cctgatccag
      961 catgccaaca agaatccggt ggatgatttc tgggcctatt cgatcaaccg cttcgagcgc
     1021 tgcaaaaagc tgatggccac ggcagaattc gggaagcacg tagcggcagt gaagaagggc
     1081 tagggccttt cacttccgcg ccatgcagga ctggtagcgc cggtccacgg tctcagcaaa
     1141 aatcctggtt gtcatcttgc tcctgatttt ggggctcgac agggcaatgt tggggaccat
     1201 ggcaaaggcc gggcgcctcc cggtggactg ttcgtaggta gcgcgcaggg cgctgtaggt
     1261 cagcgtctgc acgaagtcat tgtccttctc gcgcaggaga tccttgcgga tatcctcgtc
     1321 ggtgagttcg aaactggaat ttttgaggac ggcgcgcaaa gccatttccg ttttgctgac
     1381 gccgccactc acttttcccg atttatcata gagcaacagg tcgccatcgg cggccagggc
     1441 tgtgccgctc aattcggcga tctggctttg aagcgcggca ttgcggctgg aatagcggcc
     1501 ggcgttgaag tccgcgaagc gatatagttt ccggctatag cccgtttcat agccgagcag
     1561 caggagaacg ccgtaataca tgccgccatg gcgggtgtag agttcatcgc gcagggcgta
     1621 aacgtcatcc agagccatgg gcagccagcg ccgccgcttg gcaacttcca gggcaaaatc
     1681 gaccgacacc tgcatggagc cgatggtgtc gatctcgttg cggccatcga tctgcttgtt
     1741 gaggaggccg ctgttgatca ccaggccgag gccggtttgc ttgccggcat cggccaccat
     1801 ggcgcggtag acaagatcaa gatcgcgttc ggtgcgggcg gcgcggatgc gggcgaggta
     1861 gttatctgcg gggctaggcg tcacctccag aaaatggaga gctacgcgtc cgagaacggg
     1921 caccttgtcc atcttggcgc gcaacgccgc ttccgaaatc ttgccgagac ctgcaaccgc
     1981 aggatcagcg acgaagcttg attcctggtc aatgatggca atggcggcgc agatgtcttc
     2041 cttgctggcg ggcagattgt ggagcttcag cacatcgagc agatcatcgg cccagcccct
     2101 tgaatcctta acgctctttt cggcggcgtt gataagtctt gccaccgcag gcgtggacaa
     2161 ggccatgggt tcctctgcgc tgaaggcgga gcaggcggac caaagggcca ccagaaaaca
     2221 gatgaataac ctgatcacgc agagctcctt tccttcggac atgaacccca acaggaatta
     2281 acgtccaaaa taagaagccc tgcagaacct gccggctatt tcaggacgcc ggaagccttt
     2341 gcagcatgcg ccgcgaggta atccatgagg gccggcgaca ggcagtcgta gggttggagg
     2401 ccaacctgct tcaggcgctt gcggatggcc ggcatatcct caggctttgt tccactttca
     2461 atgaccgagg agacaaaggc cgcgaattcg ggcggcgacc agcctttttc cttggacagt
     2521 tcggtgtgga tgaaatcgag gccatagaaa ccgtgggtgg tgcgctcgac gcggccatac
     2581 atgtgaacgc cgcaaccggt gcaggcgtgg cgctggatca aggcattggc atcgacgatc
     2641 ttcagcttgt tctcattcgc cgtgaccttc acgttatcgc ggccgaccac ggcgacgacg
     2701 gagaagatgg cgccggaagg cttccagcat ttggtgcagc cgcaggcgtg gttgtgggcg
     2761 gtttgcgcgc caacacttac gatgaccgga tcggttgtgc atttgcaggt caatgtgccg
     2821 ccggcgaatt tcgccttggc cttgcccagc acgcccttgt cgagcaaggg gtgaagggaa
     2881 atcgcctttg atttcttggc ggcggctttg gttttggctt tcttggccat ggtggtcctc
     2941 cctcagtttc tgtttttaaa acacgacaac gctgcggatt gatttgcctt catgcatgag
     3001 atcgaaagcg gaattgattt tctccagcgg caggatgtgg gtgatcatgg gatcgatctc
     3061 gattttg
//
LOCUS       DAORVN010000195         3063 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_28315_length_3063_cov_2.656250, whole genome shotgun sequence.
ACCESSION   DAORVN010000195 DAORVN010000000
VERSION     DAORVN010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3063
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28315_length_3063_cov_2.656250"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2166)
                     /gene="cobN"
                     /locus_tag="PKV23_04990"
     CDS             complement(<1..2166)
                     /gene="cobN"
                     /locus_tag="PKV23_04990"
                     /EC_number="6.6.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051116 - cobaltochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobN"
                     /protein_id="HQX84825.1"
                     /translation="MHLLATTSGVIDGAAEAVDLKQSPADIVVLSAADSEIAALARAY
                     DTGKYPTLRLANFLHLQHNFSVDLYLDKTLSLARLIVLRLLGGASYWPYGLEQIEALA
                     KDNDIKLVVLPGDSQPDPDLTSRSNLPLQDCERLRQYLVAGGQFNAAKFLGYCRHLLE
                     GTEIPAPAEAPPSNGIYRSTAGKQMTGLIFYRSVLEGGQTAPIDALIDALAKRGFGCV
                     GIFVSSLKDKASTDFIHETLAGHSPSLLLNATSFAATSGGGGDPLSQYDCPVLQVVLG
                     TVSEESWRASPQGLGARDLAMNVVLPELDGRIHSRAISFKSDSFYHEPTQCRVVTYKP
                     LADRVEFVADLAANWIILRHRETINCKVGIILANYPNRDGRIANGVGYDTPASTIAML
                     KAMQEAGYDVGEIPAGGNDLIHRLASARAPQSPPDTPHDVKLSLAEYQKYFVELPYAV
                     REQITSRWDIPETDPFFRNGAFHLAVLTFGNVAIGIQPARGYNIDPKATYHDAALVPP
                     HGYLAFYFWLRHSFKAHALIHNGKHGNLEWLPGKANGLSAECYPEIAHGPLPQLYPFI
                     VNDPGEGTQAKRRTSAVIIDHLTPPLTRAETYGPLKDLEALVDEYYLASGLDRRRLAA
                     LKKDILDLTRSSRLDLDAGFTGDDDSDLTKLDAYLCDLKEAQIRDGLHILGTSPAGAQ
                     EADLLAVSQLKTTQVSDFIQERFGDARVLVMLSPIWGLAS"
     gene            complement(2166..>3063)
                     /gene="cobW"
                     /locus_tag="PKV23_04995"
     CDS             complement(2166..>3063)
                     /gene="cobW"
                     /locus_tag="PKV23_04995"
                     /inference="COORDINATES: protein motif:HMM:TIGR02475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cobalamin biosynthesis protein CobW"
                     /protein_id="HQX84826.1"
                     /translation="DGEVLKGCGDALCKEEDVIELANGCICCTVADDFVPTMKKLLDR
                     ATPIDHIVIETSGLALPQPLIRAFSWPEISARVTVDGVVAVVDAKALAEDRYADDEAT
                     IQAMRQADPKLEHENPIEELFEDQLNCADMVILNKTDLLDDEETLDVTADIEGQIRKG
                     TRILLSEHGKLDIKALLGLGSAAEGDIGNRLSHHEMEGEEQHDHDDFISFTVALPDVK
                     DRGALQRRIAEVIADSTILRLKGFANVVGSSSRLLIQAVGPRMNSYFDRPWKSGENRA
                     TRLVVIGVKGMDQQAITAALQG"
BASE COUNT          581 a          861 c         1023 g          598 t
ORIGIN      
        1 gctcgccagg ccccagatcg gcgagagcat gaccagcacc cgcgcatcgc cgaagcgctc
       61 ctggatgaaa tcggagacct gcgtcgtctt gagctgggaa accgcgagca ggtcggcctc
      121 ctgcgcgccg gcaggcgagg tgcccaggat atgcaggcca tcgcggatct gggcttcctt
      181 gagatcgcag agataggcat cgagtttcgt gagatcactg tcgtcatcgc cggtgaagcc
      241 cgcgtcgagg tccagccggc tgctgcgggt gagatcgagg atgtccttct tcagggcggc
      301 aagcctgcgg cggtcgaggc cggaggcgag gtaatattca tcgaccaggg cttccaggtc
      361 cttcaggggg ccataggttt cggcccgggt gagcggcggg gtaaggtggt caatgatcac
      421 cgccgaggtg cggcgcttgg cttgcgtgcc ttcgccgggg tcgttgacga tgaagggata
      481 gagttgcggc aatggcccgt gggcgatttc gggatagcac tcggcgctca ggccattggc
      541 cttgcccggc agccattcga gattgccatg cttgccattg tgaatcagcg catgggcctt
      601 gaagctgtgg cggagccaga aatagaaggc gaggtaacca tggggcggca cgagagccgc
      661 atcatggtaa gtggccttgg gatcaatatt gtagccgcgg gccggctgga tgccgatggc
      721 cacgttgccg aaggtgagca ccgcaagatg aaaggcgcca ttgcggaaga agggatcggt
      781 ctccggaatg tcccagcgcg aggtgatctg ttcacggacg gcatatggca gctcgacgaa
      841 atatttctgg tattccgcca ggctcaactt cacgtcgtgg ggtgtatcag gcggcgactg
      901 cggagcgcgc gcggatgcga gacggtggat caaatcatta ccgccggcgg gaatttcacc
      961 cacgtcatag cccgcctcct gcatggcttt cagcatggcg atggtgctgg ccggcgtgtc
     1021 atagcccacg ccgttggcga tgcggccatc acggtttggg tagttggcca ggatgatccc
     1081 taccttgcaa ttgatggttt cgcggtgacg caggatgatc cagttcgccg cgaggtccgc
     1141 cacaaactca acccggtcgg ccaaaggctt gtaagtcacg acgcggcatt gggtgggctc
     1201 gtggtaaaag ctgtcgctct tgaaggagat ggcgcgggaa tgaatgcggc catcgagttc
     1261 ggggagcacc acgttcatgg caaggtcgcg ggcacccagg ccttgcgggc tggcgcgcca
     1321 gctttcctcc gacacggtcc cgagaacaac ctgcagcacc gggcaatcat attgcgaaag
     1381 gggatcaccg ccgccgcccg acgtggccgc gaacgaggtg gcgttgagca gcaggcttgg
     1441 agaatgcccg gcaagagtct cgtggatgaa atcggtgctg gccttgtcct tgaggctcga
     1501 gacaaaaatg ccgacacagc caaagccccg ttttgcaagg gcgtcaatca gggcatcgat
     1561 gggcgcggtc tggccgcctt caagcacgga gcggtagaag atcagtccgg tcatctgctt
     1621 gccggcggtg ctgcggtaaa tgccattgct gggtggcgct tcggcgggcg cgggaatttc
     1681 ggttccttca agcaggtgcc ggcaatagcc gagaaacttg gcggcattga actggccgcc
     1741 ggccaccagg tactggcgca ggcgctcgca atcctgcagc ggcaggttcg aacgcgaggt
     1801 gagatcggga tcaggctggg aatcgccggg caggacgaca agcttgatgt cgttgtcctt
     1861 ggccagcgcc tcgatctgct caaggccata gggccagtag ctggcgccgc cgagcaggcg
     1921 cagcacaatc agccgggcca ggctcagggt tttgtcgaga taaaggtcga cggaaaaatt
     1981 atgctggagg tggaggaaat tggcgagacg cagggtgggg tattttccgg tgtcataggc
     2041 ccgtgccaat gcggcaatct cgctgtcggc ggcactcagc accacgatat cggcgggtga
     2101 ctgcttgagg tcgaccgctt ctgccgcccc gtcaatcacg ccacttgtcg tggcgaggag
     2161 gtgcatcagc cctgaagggc cgcagtgatc gcctgctggt ccatgccttt tacgccgatc
     2221 accacaagtc tggtggcgcg gttctcacca cttttccagg gcctgtcaaa ataactgttc
     2281 atgcggggtc ctacggcctg aatcagcaac cgtgacgaag accctacaac attggcgaaa
     2341 cccttcaatc gcaaaatggt gctgtcggcg atcacctcgg cgatgcgcct ctgcagcgcg
     2401 ccgcggtcct tcacatcggg aagggcaacg gtgaagctga tgaaatcatc atggtcgtgc
     2461 tgctcctcgc cttccatttc gtgatgcgaa agccggttgc caatgtcgcc ctctgcggcg
     2521 gaaccaaggc caagcagggc cttgatgtcg agtttgccat gctcgctcag caggatgcgg
     2581 gtgcccttgc ggatctgccc ttcgatgtcg gcggtgacat cgagggtttc ctcgtcgtcg
     2641 agcaggtcag tcttgttgag gatcaccatg tcggcgcagt tcaactgatc ctcgaacaac
     2701 tcctcgatgg ggttttcatg ctcgagcttg ggatcggcct ggcgcatggc ctgaatggtg
     2761 gcctcgtcgt cggcatagcg gtcctcggcg agggccttgg catcaaccac ggccaccacg
     2821 ccatcaacgg tgacgcgggc ggaaatctcc ggccagctga aggcgcggat gagcggctgg
     2881 ggcagggcaa ggcccgaggt ttcgatcacg atgtgatcga tgggcgttgc acggtcgagg
     2941 agctttttca tggtcgggac aaaatcatcg gccacggtgc agcagatgca tccgttggcg
     3001 agttcgatga catcttcctc cttgcacaga gcatcgccgc agcctttcaa cacttcgccg
     3061 tcc
//
LOCUS       DAORVN010000196         3054 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_28458_length_3054_cov_3.763588, whole genome shotgun sequence.
ACCESSION   DAORVN010000196 DAORVN010000000
VERSION     DAORVN010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3054
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28458_length_3054_cov_3.763588"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1892)
                     /locus_tag="PKV23_05000"
     CDS             complement(<1..1892)
                     /locus_tag="PKV23_05000"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006937963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HQX84827.1"
                     /translation="MTPTSVDSFKCRKTLKVGAKNYTYFSLKAAEKNGLKGISKLPYS
                     LKVLLENLLRHEDGRSVTKADIQGIADWLKNRGKDEMEIAFRPARVLMQDFTGVPAVV
                     DLAAMRDAMKDLGQNPTKINPLAPVDLVIDHSVMVDYFASTGAFKKNVEMEYGRNGER
                     YTFLKWGQGAFDNFRVVPPGTGICHQVNLEYLAKTVWTRTDKVKGKQVTTAYPDTLVG
                     TDSHTTMINGLAVLGWGVGGIEAEAAMLGQPISMLIPEVIGFKLDGRLPEGTTATDLV
                     LTVTQMLRKKGVVGKFVEFYGPGLGTLALEDMATIANMAPEYGATCGFFPVNAETISY
                     LKGTARPAAQVALVEKYAKAQDMFWTPNSIDPDFTDVLSLDLATVVPSMAGPKRPQDK
                     VTLTESASGFAKVMADEFKKLEQLGQRFKVENANFDVGHGDVVIAAITSCTNTSNPSV
                     MIGAGLLARNAQRKGLTVKPWVKTSLAPGSQVVTEYLKSSGLQKDLDKLGFDLVGYGC
                     TTCIGNSGPLPENISESIRKNDLVAAAVLSGNRNFEGRVNPDVRANYLASPPLVVAYA
                     LAGSLNVNLTTDPLGFDKKGKPVYLKDIWPDAKTVADFVRKNVTRKAFAKRYKDVFKG
                     DAAWRK"
     gene            complement(1975..2751)
                     /locus_tag="PKV23_05005"
     CDS             complement(1975..2751)
                     /locus_tag="PKV23_05005"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006467970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (ubiquinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HQX84828.1"
                     /translation="MVEFALPQNSKVQPGKSWPKPAGKKLQEFRIYRWNPDDGKNPQI
                     DSYHVDMESCGPMVLDAIIKIKNEQDPTLTFRRSCREGICGSCAMNIDGTNTLACLKA
                     CDEVKGPIRIYPLPHMPVVKDLVPDLTRFYQQHKSVEPWLKTTSPTPEKEWKQSHSDR
                     ALLDGLYECILCACCSTSCPSYWWNGDRYLGPAVLLQAYRWLIDSRDEAKMHRLDRIE
                     DPFKLYRCHTIMNCAKACPKGLNPAKAIAEIKKLMVERQI"
     gene            complement(2839..3054)
                     /locus_tag="PKV23_05010"
                     /pseudo
     CDS             complement(2839..3054)
                     /locus_tag="PKV23_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004446645.1"
                     /note="part of four member fumarate reductase enzyme
                     complex FrdABCD which catalyzes the reduction of fumarate
                     to succinate during anaerobic respiration; FrdAB are the
                     catalytic subcomplex consisting of a flavoprotein subunit
                     and an iron-sulfur subunit, respectively; FrdCD are the
                     membrane components which interact with quinone and are
                     involved in electron transfer; the catalytic subunits are
                     similar to succinate dehydrogenase SdhAB; frameshifted;
                     incomplete; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     flavoprotein subunit"
BASE COUNT          553 a          975 c          859 g          667 t
ORIGIN      
        1 atcttgcgcc acgccgcgtc gcccttgaac acatccttgt agcgcttggc aaaagccttg
       61 cgggtgacgt tcttgcgcac gaagtccgcc acggtcttgg catccggcca gatgtccttg
      121 aggtaaaccg gcttgccctt tttatcgaag ccaagcggat cggtggtgag gttcacattg
      181 agcgatcccg ccagggcata ggccactacc agcggcggag aagccaggta attggcccgc
      241 acatccggat tcacccggcc ttcgaaattg cggttgcccg aaagcaccgc cgcagcaacc
      301 aggtcgtttt tccggatcga ctcggaaatg ttttcaggca agggccccga gttgccgatg
      361 caggtcgtgc agccatagcc caccaggtcg aagcccagct tatccaggtc cttctgcagg
      421 cccgatgact tcagatattc cgtcaccacc tggcttcccg gcgccagcga tgtcttcacc
      481 catggcttca ccgtcaggcc cttcctctgc gcattgcgcg ccagaagacc cgccccgatc
      541 atcaccgaag gattggaagt gttggtgcac gacgtgatcg cggcgataac aacgtcgcca
      601 tggcccacat cgaaattggc attctccacc ttgaagcgct ggccgagctg ttccagtttc
      661 ttgaattcat cggccatcac cttggcaaag ccggaagccg actccgtcag cgtcaccttg
      721 tcctgcggcc gcttgggccc ggccatggaa ggcacgactg ttgcaaggtc aagcgacagc
      781 acatcggtga aatctggatc gatggaattc ggcgtccaga acatgtcctg cgccttggca
      841 tatttttcga ccagcgccac ctgggcggct ggccgcgccg tgcccttgag ataggaaatg
      901 gtctccgcgt tcaccgggaa gaagccgcag gtcgcgccat attccggcgc catgttggca
      961 atcgtcgcca tgtcttccag cgccagcgtg ccaagccccg gcccgtagaa ttccacgaat
     1021 ttcccgacca cgcctttctt gcgcagcatc tgcgtcaccg taaggaccag gtcggtcgcc
     1081 gtcgtgcctt ccggcaaacg gccgtcaagc ttgaagccga tcacttccgg aatgagcatc
     1141 gaaatcggct gccccagcat cgccgcctcc gcctcgatgc cgccaacccc ccagcccaat
     1201 acggcaaggc cattgatcat cgtggtgtgg ctgtcggtgc ccaccagcgt atcaggatag
     1261 gcggtcgtca cctgcttgcc cttcaccttg tcggtgcgtg tccacaccgt cttggccaga
     1321 tattccaggt ttacctggtg gcaaatgccg gtgcccggag gcaccacgcg gaaattgtcg
     1381 aaggcgccct gcccccattt caggaaggtg tagcgctcgc cgttccggcc atattccatc
     1441 tcgacattct tcttgaacgc tcccgtcgag gcaaaataat ccaccatcac cgaatggtcg
     1501 ataaccaggt caaccggcgc cagcggattg atcttggtgg ggttctggcc caggtccttc
     1561 atcgcatcgc gcatcgccgc cagatcgaca accgccggaa ccccggtgaa atcctgcatc
     1621 agcacgcggg ccgggcggaa ggcgatttcc atctcgtcct tgccccggtt cttcagccag
     1681 tcggcaatcc cctggatgtc ggccttggtg acgctgcgcc cgtcctcatg gcgcagcagg
     1741 ttttccagca gcaccttcag cgaatagggc agctttgaaa tgcccttgag gccgtttttc
     1801 tccgccgcct tgaggctgaa ataggtgtag ttcttcgccc ccaccttcag ggttttgcgg
     1861 cacttgaaac tgtcgacgga ggtgggggtc atgctggctt tcctgggctg gaacttcggg
     1921 gctggctata tctcttattc tcgtgcggca caattctgac aacaataaaa agggctaaat
     1981 ctgccgctcc accatcagct tcttgatctc tgcaatggcc ttggccgggt tcagcccctt
     2041 ggggcaggcc ttggcgcaat tcatgatggt gtggcagcgg taaagcttga acgggtcctc
     2101 aatcctgtcg aggcggtgca ttttggcctc gtcgcgcgag tcgatcagcc aacggtaggc
     2161 ctggagcaga accgcggggc cgagatagcg gtcgccgttc caccagtagg acgggcacga
     2221 ggtcgagcag caggcgcaga gaatgcattc ataaagcccg tcgagcagcg cccggtcgct
     2281 gtgcgactgc ttccattcct tttccggcgt cgggctcgtg gtcttcagcc acggctcaac
     2341 cgatttgtgc tgctgataaa accgcgtgag gtccggcacc aggtccttca ccaccggcat
     2401 gtgcggcagc ggataaatcc ggatcggccc cttcacctcg tcgcaggcct tcaggcacgc
     2461 cagcgtattg gtgccgtcaa tgttcatcgc gcaggagccg caaatccctt cccggcacga
     2521 acggcggaag gtcagcgtcg ggtcctgctc attcttgatc ttgatgatgg cgtccagcac
     2581 catgggcccg cagctctcca tgtccacatg gtagctgtcg atttgcgggt tttttccgtc
     2641 atccggattc caccggtaaa tccggaactc ctgcagcttt ttgccagcgg gcttcggcca
     2701 gctctttccc ggctggacct tggagttctg tggaagcgca aattcaacca tgattgctgt
     2761 tcttctcttc ccctctcgcc ttgagggaga gggtggcgcg aagcgccggg tgaggggtct
     2821 gcttcatcgt gattaccatc aatacaccct cgcctttggc ttgatgtagt caatgtcctt
     2881 cgacatggtg aaatcatgca ccgggcggta ggcgagcttc accttgcgct tggcgccatc
     2941 ggtccagctc agggtatgct tcatccagcc cttgtcatcg cgctggggaa aatcctcgcg
     3001 cgcatgcgcc ccgcggcttt cctcgcgcgc caggcgcctt caacggtcac cgcc
//
LOCUS       DAORVN010000197         3053 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_28473_length_3053_cov_2.992328, whole genome shotgun sequence.
ACCESSION   DAORVN010000197 DAORVN010000000
VERSION     DAORVN010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3053
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28473_length_3053_cov_2.992328"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(60..947)
                     /locus_tag="PKV23_05015"
     CDS             complement(60..947)
                     /locus_tag="PKV23_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007197944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQX84829.1"
                     /translation="MNTATPPVRVIKARRSVFENIRRNLKTAPLTAWFGMIVILLYAI
                     CAIFAPLLAPYGESEIVGKAYQLSSAENWLGTDQIGRDTLSRLIFGARNTIGIALITT
                     LLSFLIGGSLGVLAALKRGVLDQGISRVVDAFLAIPVLIFALMMLAVVGKSLINLIII
                     LALLDATRVFRLSRAVALNSVVMDYVESARLRGEGMSWIVFKEIVPNILPPLITEFGL
                     RFCFVFLTISALSFLGVGIQPPTADWGSMVAESKSLISFGRPAPLYPAAAIALLTVAV
                     NFVVDWFLHKTSGLRDGQH"
     gene            complement(944..1915)
                     /locus_tag="PKV23_05020"
     CDS             complement(944..1915)
                     /locus_tag="PKV23_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006935299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQX84830.1"
                     /translation="MHPVLRTVLQRLGLGIVTLLAVSVIIFSSLEMLPGGYAENVLGQ
                     SATPETVAAFKRDLGLDRPATVRYFEWIGKAVQGDFGSSYSGLSGSMKRDVSTTIAPR
                     LYNTFFLAAMAALIAVPLSLLLGAMAALYRNSWFDRIVNSVTLTTIAMPEFFIAYILV
                     YVFAVKLKLFYPNSTVSIGTPFVEHIYRAALPAITMTLVIIAHMMRMTRAAIINLLAS
                     PYIEMAQLKGVSKSDVIRHHALPNAWAPIATVIAFNLAYLVVGVVVVEVVFVYPGIGQ
                     LMVDAVSSRDMPVVQACALIFAATYILLNLLADIVSIVTNPRLLHPK"
     gene            complement(2098..>3053)
                     /locus_tag="PKV23_05025"
     CDS             complement(2098..>3053)
                     /locus_tag="PKV23_05025"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HQX84831.1"
                     /translation="LKSYEPGVKLVGERNPNFFKDTWFDGIEMITIADVAARTNAYLS
                     GEVHFIDRADLKTIDMLKSAPNTEIYNQSGPTPYTAPMLCDAAPFDNPAVRKALKYAI
                     NRQEMVDKVLYGYGKVGNDNPIGSQMKYAIDPQPQHTYDPEKAKSILKEAGIENLKVD
                     FSCPPNAAFPGALDAALLMQESAKAAGIDINVIREPDDGYWSNVWLKKPWSMCYWGGR
                     PTVDTMLSISLAADAPWNDTHFKNARFNELLVQARSELDETKRAAMYAECQQIVHDEG
                     GQVVLMFYNFVGALNTAVAHGELNSDQDSDGGYMVERWWMA"
BASE COUNT          702 a          813 c          946 g          592 t
ORIGIN      
        1 agccatcaat cttgagaccc ttgatgtcga gaagggtttc gcccttgacg cgcttcttat
       61 cagtgctgac catcgcgaag cccacttgtt ttatggagga accaatcaac tacgaaatta
      121 accgcaacgg tgagaagcgc gattgccgcg gcaggataaa gcggtgccgg cctgccgaaa
      181 ctgatcagcg acttgctttc agccaccatt gacccccaat cggcggtggg cggctggatg
      241 ccgacaccaa ggaaggagag cgcagaaatg gtgaggaaca cgaaacagaa gcgcaggcca
      301 aattcggtga tgagcggcgg caggatattg gggacgatct ccttgaaaac aatccaactc
      361 atgccttcac cgcgaagcct ggcagattca acgtaatcca tcaccacgga attcagggcc
      421 acggcgcggg acaggcggaa gacgcgggtt gcgtcgagca aggccagaat gatgatgagg
      481 ttgatgaggc tcttgccaac cacggccagc atcataaggg cgaagatgag cacgggaatg
      541 gcaaggaagg catcgacgac acgcgaaatg ccctggtcga gaacgccgcg cttcagggcg
      601 gcaagcacac ccagtgaccc cccgatgaga aaagacagga gagtggtgat gagggcaatg
      661 ccgatggtgt tgcgggcgcc gaaaatcagg cgcgagagcg tgtcacggcc aatctggtcg
      721 gttcccagcc agttttccgc gcttgaaagc tggtaggcct tgccgacgat ctccgactcg
      781 ccgtagggcg cgagaagggg agcgaagatc gcgcaaattg catagagcag aatgacgatc
      841 atcccgaacc aggcagtcaa gggcgcggtc ttcaggttgc gcctgatatt ctcgaagacc
      901 gagcggcggg ccttgatgac gcggacaggc ggtgtggctg tgttcatttt ggatgcaaca
      961 atcttggatt ggttacaatt gagacgatgt cggccaacag gttgagcaaa atgtaggtcg
     1021 cggcgaatat cagggcgcag gcctggacaa ccggcatgtc gcggctggac acggcgtcaa
     1081 ccatgagctg gccgatgccg ggatagacga acaccacttc aaccaccacc acaccaacca
     1141 ccagataggc gaggttgaag gcaataacgg tggcaatggg ggcccaggca ttgggaagcg
     1201 catgatgcct gatcacatcg gatttcgaga cgcccttgag ctgcgccatt tcaatgtaag
     1261 ggctggccag caggttgatg atcgcggccc tggtcatgcg catcatgtgg gcgatgatta
     1321 ccagcgtcat ggtgatggcg ggcagcgccg cccggtagat atgctcgacg aacggggttc
     1381 cgatgctgac tgtcgagttg gggtaaaaca gtttcagctt cacggcgaaa acatacacca
     1441 ggatataggc aatgaagaat tccggcatgg caatggtcgt cagggtgacg gagttgacga
     1501 tgcggtcaaa ccaggaattg cgataaaggg ccgccattgc ccccagcaag agggaaagag
     1561 gaacggcgat aagggcggcc atggccgcca gaaagaaggt gttgtagagg cgcggcgcaa
     1621 tggtggttga cacatcgcgc ttcatgctgc cgctcaagcc ggaataggaa gaaccgaaat
     1681 cgccctggac ggccttgccg atccattcga aatagcggac ggtggcgggg cggtcgaggc
     1741 caaggtcgcg tttgaaggcg gccacggttt caggcgtggc gctctggccc aggacatttt
     1801 cggcgtagcc gcccggcagc atttcaagcg acgagaaaat gatcaccgaa acggcaagca
     1861 gcgtaacgat gcccaagccc aaacgctgca gaacagttct cagaactgga tgcattcatc
     1921 cccctttttt gggcttcttt ttcggccccg tttttttaac cctagcagaa cggtaaaaca
     1981 ccgccacaaa aaaaccgtat ggcggcatgc gacccattgc gacagttgag cgacgaaagc
     2041 aaaaaaggcc ccgccggatt tccggcgagg cccgtaatcg agatcgtcag ccgtggatta
     2101 cgccatccac cagcgttcaa ccatatagcc gccgtcgcta tcctggtcgg agttcaactc
     2161 tccgtgggca accgcggtgt tcaaggcgcc gacgaagttg tagaacatca gcacaacctg
     2221 gccgccttca tcatgaacaa tctgctggca ttcggcatac atggctgcgc gctttgtttc
     2281 gtcaagctcg ctgcgggcct gaaccagaag ttcgttgaag cgggcattct tgaaatgcgt
     2341 gtcattccaa ggcgcatcag ccgcaaggga gattgacagc atcgtatcca ccgtcggacg
     2401 gccgccccag tagcacatgc tccaaggctt cttgagccag acgttggacc aatagccatc
     2461 atccggttcg cggatcacgt tgatatcgat gcctgccgcc ttggcgcttt cctgcatcag
     2521 cagggctgcg tcgagggcgc cggggaatgc ggcgtttggc ggacaggaga aatcgacctt
     2581 gagattctcg atgccggctt ccttcaggat cgacttggcc ttttccggat cgtaggtatg
     2641 ctgcggctgc ggatcgatgg catatttcat ctgcgagccg atcgggttgt cattacctac
     2701 cttgccgtag ccatacagca ccttgtccac catttcctgg cggttgatgg cgtatttgag
     2761 ggccttgcgc acggcgggat tgtcgaacgg cgctgcatcg cagagcatcg gcgcggtgta
     2821 tggcgtcggg ccgctctggt tgtagatttc cgtgttcggc gcgctcttca gcatgtcgat
     2881 ggtcttgaga tcggcacggt cgatgaagtg cacttcgccg gagaggtagg cgttggtgcg
     2941 ggcggcgaca tcggcaatgg tgatcatttc gatgccgtcg aaccaggtgt ccttgaagaa
     3001 gttggggttg cgttcaccaa ccagcttcac gcccggctcg taggacttga gaa
//
LOCUS       DAORVN010000198         3046 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_28572_length_3046_cov_2.606486, whole genome shotgun sequence.
ACCESSION   DAORVN010000198 DAORVN010000000
VERSION     DAORVN010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3046
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28572_length_3046_cov_2.606486"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            188..1081
                     /locus_tag="PKV23_05030"
     CDS             188..1081
                     /locus_tag="PKV23_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HQX84832.1"
                     /translation="MAELQARDDGLYRLGFAGDTLNTAWYMRALTRPQDVAVDYVTAV
                     GTDPLSAKMLAFLKANGVGTRFIQEVSDRTVGLYLITLAGAERSFTYWRSSSAARLLA
                     ENREVLAASLSHADAIYFSGITLAILSPAHRRSFLALLQEMTKRGATIAFDSNARRRL
                     WPSDRAMKAAMIEGYKVCTLALPTFSDDQALFGDATPSDCAKRIAGYGVHEIVVKDGS
                     NPCVVSLDTKQFSVAPDAVQEIVDTTGAGDSFNAGYLAARLLNTDPLEAAKLGHRVAG
                     RVIRERGALLAMASFSDLVAG"
     gene            1140..2696
                     /locus_tag="PKV23_05035"
     CDS             1140..2696
                     /locus_tag="PKV23_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019857501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HQX84833.1"
                     /translation="MINTMELAKQVTATGRRSGGREGRRALRAVPMASFPTLVRNIPT
                     YQMVPDEAVELIHDESMKILEEVGCEFRDDEAISMWKAAGADVTGTRVRIDQALLMSL
                     VAKVPPEFTLNARNPERTVKVGGKNTVFVPMYGAPYVRGLDGERRYGSLADLNNLHKL
                     AYMSPALHSSSSIICEPMEIPVPKRHLHIIHSALKHSDKPFMGIVTSKERAEDVMKMS
                     GIVFGEDYVKDNTVVVSITNCNSPLVWDATMLDAMKVYARHNQPLILAPFALCGASTS
                     ASSIGAVAQVNAEALAGVAFTQLIRPGSPQIYGQFMVTVDMKTGAPMGGTPEAAQMMF
                     VMGALARKYNLPWRTSGFHVGSKLNDAQAGYESNMLMHAAILSGANYIWHVAGWLEAG
                     LCCGFSKFVTDAEQLQGWYKYAQGPSFADFKEAMAAIREVGPAGHFLGTKHTLEHFED
                     AFFMPSVMDFNPFEQWSAEGAKDHDARGRDKAAAMLAAYEEPAMDLGIADGLKDFIAR
                     RESELPNTVS"
     gene            2693..>3046
                     /locus_tag="PKV23_05040"
     CDS             2693..>3046
                     /locus_tag="PKV23_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HQX84834.1"
                     /translation="MKDLQIFVDADACPVKEEAAKVAARHKLVIHYVSNSHMRLPFGD
                     NVKRVVVSDGADAADDWIAEQCAAGDIVVTADIPLASRAIKKGAQVISHIGKPFTQDS
                     IGMALAMRDLMAQLRE"
BASE COUNT          603 a          863 c          989 g          591 t
ORIGIN      
        1 cctgcaaaaa ctggtggacg agaacatcgc cagcggcatg ctcaagcaag accccaacga
       61 tccgtcctgg aagcactttc agcgccgcac gcaggccctg caagtgcaga agctgcgcgt
      121 ctacgccaag gaaggactca ttgacggcgg cgaggccgca cagcagctca tcacgggcgg
      181 cgccagcatg gcggaactgc aggccaggga tgacgggctt taccgtctgg gctttgccgg
      241 cgacaccctc aatacggcct ggtacatgcg ggctctgacg cgcccgcagg atgtggcggt
      301 ggattatgtg acggcggtgg gcaccgaccc cctgtcagcg aaaatgctgg cctttctgaa
      361 ggccaatggc gtcgggaccc ggttcataca ggaggtttcc gacaggacgg tgggactcta
      421 cctgatcacg cttgccgggg ccgaacggag tttcacctat tggcgcagtt cctcggcggc
      481 gcggctgctt gctgaaaaca gggaggtctt ggcggcaagc ctttcgcatg ccgatgcgat
      541 ctatttttcc gggatcacgc tggccattct ttcacccgcc catcggcgaa gttttttggc
      601 gctgttgcag gagatgacaa agcggggagc cacgatcgcc tttgattcaa atgcgcggcg
      661 ccggctttgg ccgtccgacc gggcgatgaa ggcggcgatg attgaaggtt acaaggtttg
      721 caccctggcg cttcccacat tcagcgacga ccaggccttg ttcggggatg ccacgccttc
      781 ggattgcgcc aaacgcattg cgggctacgg ggttcacgaa attgttgtga aagatggcag
      841 taatccctgt gtggtgtccc tggacacaaa gcaattttcc gtggcgcccg atgcggttca
      901 ggaaatcgtc gatacgacag gggccggtga cagtttcaat gcgggctatc tggcggcgcg
      961 cctgctgaac acggacccgc tggaggcggc aaagcttggg caccgcgttg cgggccgggt
     1021 catccgggag cgtggcgcac ttctcgccat ggcgtcgttt tccgatcttg ttgcagggtg
     1081 attggtgttg attcgtttcc ccgcccggtg ctcaatggtt cgatgaaagc gggtgcgcaa
     1141 tgatcaatac gatggaactg gcaaagcagg tgacggccac gggcaggcgg agcggcgggc
     1201 gcgaaggccg gcgggccttg cgggcggttc ccatggcctc gtttccaacg ctggtgcgca
     1261 atatcccgac ctaccagatg gtgcccgacg aggcggtgga gctcattcac gacgagtcga
     1321 tgaaaatcct cgaggaagtc ggctgcgaat tccgcgatga cgaggccata tccatgtgga
     1381 aggcggcggg ggctgacgtt acgggcacgc gggtgcggat cgaccaggcg ctgctgatga
     1441 gcctggtggc gaaagtgccg ccggagttca cgctcaatgc gcgcaatccg gagcgcacgg
     1501 tgaaggtggg cggcaaaaac acggtgttcg tgccaatgta tggcgcgcct tacgtgcgcg
     1561 ggctggacgg tgaacggcgc tatggctcgc tggccgatct caacaacctt cacaagctgg
     1621 cctacatgtc gccggccttg cattcctcca gctcgatcat ctgcgagccc atggaaatac
     1681 ccgtgcccaa gaggcacctg catatcattc attcggcgct gaaacattcc gacaagccct
     1741 tcatgggcat cgtcacgtca aaggagcggg ccgaggacgt gatgaaaatg tcgggcatcg
     1801 tgttcggcga agattatgtg aaagacaaca cggtggtggt gtcgatcacc aattgcaatt
     1861 cgccgctggt gtgggatgca accatgctgg acgcgatgaa agtttacgcg cggcataatc
     1921 agccgctgat cctggcgccg ttcgcgctgt gcggggcttc gacgtccgcc tcttccattg
     1981 gcgcggtggc gcaggtgaat gcggaagcac tggctggcgt cgccttcacc cagctcatcc
     2041 gcccgggcag cccgcagatc tacggccagt tcatggtgac ggtggacatg aaaaccggcg
     2101 ctcccatggg tggcacgccg gaagcggcgc agatgatgtt tgtgatgggg gcgctggcgc
     2161 ggaaatacaa tctgccctgg cgcacctcgg gattccatgt gggctcgaag ctcaatgacg
     2221 cgcaggccgg ctatgaatcc aacatgctga tgcatgcggc gattctttca ggcgccaact
     2281 acatctggca tgtggcgggc tggctcgagg cgggcctatg ctgcggcttc tccaaattcg
     2341 tgaccgacgc ggagcagctc cagggctggt ataaatatgc gcaaggcccc tccttcgctg
     2401 atttcaagga ggctatggcg gccatccgcg aagtggggcc agccgggcat ttcctcggca
     2461 ccaaacacac gcttgagcat tttgaagatg cgtttttcat gccatcggtc atggatttca
     2521 atccgttcga acagtggtca gcggagggtg ccaaggacca tgatgcaagg ggccgcgaca
     2581 aggccgccgc catgctcgca gcctatgaag aacccgccat ggacctgggg attgccgacg
     2641 gactgaagga cttcatcgcg cggcgcgaat ccgaattgcc gaatacagtg agttgaaaga
     2701 tttgcagatc ttcgtggatg cggatgcctg cccggtaaag gaggaggcag cgaaagtcgc
     2761 ggcgcggcac aagcttgtca ttcactatgt atccaactcc cacatgcggc tgcctttcgg
     2821 cgacaacgtc aaacgcgtcg tcgtttccga tggggcagac gctgccgacg actggattgc
     2881 ggagcaatgc gccgccggcg atattgtggt cacagcggat attccccttg cctcgcgggc
     2941 aatcaagaaa ggggcgcagg tcatctcgca catcggaaag cccttcaccc aggacagcat
     3001 cggcatggct ctggcgatgc gcgatctgat ggcgcaactg cgtgaa
//
LOCUS       DAORVN010000199         3039 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_28679_length_3039_cov_3.593164, whole genome shotgun sequence.
ACCESSION   DAORVN010000199 DAORVN010000000
VERSION     DAORVN010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3039
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28679_length_3039_cov_3.593164"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="PKV23_05045"
     CDS             complement(<1..181)
                     /locus_tag="PKV23_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007607851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HQX84835.1"
                     /translation="MHFTDIFVKRPVLATVVSLLILLAGLQAFFQLPIRQFPEVADTK
                     IEITTTYPGANADQIK"
     gene            complement(191..1333)
                     /locus_tag="PKV23_05050"
     CDS             complement(191..1333)
                     /locus_tag="PKV23_05050"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HQX84836.1"
                     /translation="MIRRSFYFLLVTVILGGLAGGIAWWSFYALPAFIAQSIQSTPAP
                     VQTVSAEEAKAESWQPEIAGIGTLTASEGIDIAPQVGGVITEVLFESGASVKKGDKLV
                     QLDTATDEADLKNLQVQLANAEEELNRKQQIFDRGFAAKADLDGLRTARDQLLANIER
                     IQAQIAQKSIYAPWDGKLGLRSISVGSYVAPGQKVVWLQKVDPIYADFDVTEEDYGRI
                     ADGLKVTARFNAWPDTPFQGSVTTTDSRMSESSRMITVRAKLDNPDGRLLPGMYANVL
                     VEAGAPQDVITVPQTAVTYTLYGDNVFVVVPDKKPDPNNKGEQLEIERRFVKAGSMRN
                     GRVQITEGLKPGDRVVTAGHNKIDQGAKVRIDNSVALREADSATIQ"
     gene            complement(1350..1886)
                     /locus_tag="PKV23_05055"
     CDS             complement(1350..1886)
                     /locus_tag="PKV23_05055"
                     /inference="COORDINATES: protein motif:HMM:NF015513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HQX84837.1"
                     /translation="MNVRTLCLGILSLKEASGYEIKKGVEDGVFCHFIDASFGSIYPA
                     LTQLAGEGFVTVREEEQTGKPDKKVYAITEAGRQALAKAISVVPAKDKYRSEFLFQML
                     LQDYLSPEMMLVAIQKQLNDLNEDLGRIAECSQKTPVGHGSHFVADYGNAVLTAAVKC
                     LEYKKAELVQHMAARAAE"
     gene            2233..>3039
                     /locus_tag="PKV23_05060"
     CDS             2233..>3039
                     /locus_tag="PKV23_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011663086.1"
                     /note="LivHMGF is the membrane component of the LIV-I/LS
                     branched-chain amino acid transporter; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease LivH"
                     /protein_id="HQX84838.1"
                     /translation="MEYFLQQLINGITLGSIYGLIAIGYTMVYGIIGMINFAHGDVFM
                     VGGFVSLIAIVALGITGGSGFFPIILALVLVVFIAMIIASAYNWTVERVAYRPLRGSF
                     RLAPLISAIGMSIVLQEFVRIAQDGRPKPLQPIVTGGYEIMSRAAAQGEFIVRLSNMQ
                     IFIIITTLILMAIFTAIITRTSLGRAQRSCEQDMKMASLLGIDINKTISITFIMGAAL
                     AAVAGLMFFLYYGVTDFYVGFLAGIKAFTAAVLGGIGSIPGAMIGGLIIGL"
BASE COUNT          602 a          870 c          842 g          725 t
ORIGIN      
        1 ccttgatctg atcggcgttg gcgccgggat aggttgtggt gatctcgatc ttggtatcgg
       61 cgacctcggg aaactggcgg atcggcagct ggaaaaacgc ctgcaggcct gccagcagga
      121 tgagcaggct caccaccgta gccaatacgg gccgctttac gaaaatgtcg gtgaaatgca
      181 tgaccctgcc ttactgtatc gtcgcgctgt cggcttcccg gagcgcaacc gagttgtcaa
      241 ttctgacctt cgcgccctgg tcgatcttgt tgtggccggc ggtcaccacc cggtcgcccg
      301 gcttgaggcc ttcggtaatc tgcacccgcc cgttacgcat ggagccagcc ttgacaaacc
      361 ggcgctcgat ttcgagctgc tctcccttgt tgttggggtc gggcttcttg tcgggcacga
      421 caacgaaaac attgtcgcca tagagcgtgt aggtcacggc ggtctgcggc acggtaatga
      481 cgtcctgcgg ggcgccagcc tcaaccagga cattggcata catgccaggc aacaaccggc
      541 catccgggtt gtcgagtttg gcacggaccg tgatcatccg gcttgattcc gacatccgcg
      601 aatccgtcgt cgtgacggag ccctggaacg gggtatcggg ccaggcgttg aaccgcgctg
      661 ttactttcaa cccgtcggcg atccggccgt agtcctcttc ggtcacatcg aaatcggcat
      721 aaatgggatc aaccttctgc agccacacaa ctttctggcc gggcgccaca tagctgccca
      781 ccgaaatgct gcgcaggcca agcttgccgt cccacggcgc gtaaatggat ttctgggcaa
      841 tctgcgcctg gatgcgctca atgttcgcca gcagctggtc gcgcgccgtg cgaaggccat
      901 cgaggtcggc cttggcggcg aaaccgcggt cgaaaatctg ctgcttgcga ttgagttctt
      961 cttcggcgtt tgccagttgc acctgcaggt tcttgagatc ggcttcgtcg gttgccgtat
     1021 cgagctgcac gagtttgtcg ccctttttca cgcttgcgcc ggattcgaac aggacttcag
     1081 taatcacgcc gccgacctgc ggagcgatgt cgatgccctc tgatgcggta agcgtgccga
     1141 tgccggcaat ctcaggctgc cagctttcag ctttggcttc ttcggcggac actgtctgaa
     1201 ccggagccgg cgtgctctgg atggattggg caatgaaggc cggcaaggca tagaacgacc
     1261 accaggcgat accgccggcg agaccgccca ggatgacggt gacgagaaga aaataaaacg
     1321 agcggcgtat catgacaaca gtctccaaat cattcggcgg cgcgggcagc catatgctgc
     1381 acaagttcgg cttttttata ctccaggcat ttgacagcag ctgtcagcac ggcgttgcca
     1441 taatcggcaa caaagtgcga cccatggcca accggggtct tttggctgca ttcagctata
     1501 cgtccaaggt cttcattgag atcattcagc tgcttttgaa tggccacaag catcatttcc
     1561 ggtgacaggt aatcctgcaa cagcatttgg aagagaaact cggagcggta tttgtccttg
     1621 gccggcacca ccgaaatagc cttcgccagc gcctgccttc cggcttcggt tatggcgtaa
     1681 acctttttgt cgggttttcc ggtttgctcc tcctcgcgga cggtcacaaa gccttcgccc
     1741 gccagttgcg tcagggccgg atatatggag ccgaagctcg cgtcaatgaa atggcagaaa
     1801 acaccgtctt ccacgccctt cttgatctcg taacccgagg cctccttgag ggacaggatt
     1861 ccaaggcata gggtgcgtac gttcattatt atggcaatcc gatatatggc aatttgatat
     1921 aggtcccgca tatatatcaa atcgatatat cgtcaaggcc aaatttttcg gtgaggggga
     1981 aaaacccgat ttagggaaat atgggggctg gagcgcgcgg ttccaagccc ctgtttcgga
     2041 gttttttgcg cataattttt aggcaggcgt cgcgccggcc tttgacacac tgctggaacc
     2101 tattgaattt aaaatgaaat tcaagttgta aatgcagctt gtataaaatt ttggaagtct
     2161 ttaattaccg actccaagta cgcagccttc aaacgtgctg cgacctgggc aggcagaaac
     2221 ataggggggg caatggagta ttttctccag cagctcatta acggcattac tctcggttca
     2281 atttacggtc tcatcgccat cggttacacg atggtttatg gcattatcgg catgatcaac
     2341 ttcgctcatg gcgatgtatt catggtgggt ggctttgtgt cgctcattgc catcgtggcc
     2401 ttgggaatca ctggaggaag cggtttcttc ccaatcattc tggctctggt cctggtcgtt
     2461 ttcatcgcca tgatcattgc atcggcctat aattggacgg ttgaacgcgt cgcctaccgg
     2521 cctctgcgcg gctctttccg cctggctccc ctgatctcag ccatcggcat gtcgatcgtg
     2581 cttcaggaat ttgtccgcat cgcccaggat ggccggccga agccgctcca gcccatcgtc
     2641 acgggcggct atgaaatcat gagccgcgcc gccgcccaag gcgaattcat cgtccgcctg
     2701 tcgaacatgc aaatcttcat catcatcaca actctcatcc tgatggccat cttcaccgcc
     2761 atcatcaccc gcacttcgct tggccgggcg cagcgttcct gcgagcagga catgaagatg
     2821 gcttccctgc tcggcattga catcaacaag acgatctcga tcaccttcat catgggagca
     2881 gcgctggctg ccgtcgcggg cctgatgttc tttctctatt atggcgtcac cgacttctac
     2941 gtgggcttcc tcgccggcat caaggcattt acggcggcgg ttctcggcgg catcggctcc
     3001 atccccggcg ctatgatcgg cggcttgatc atcggtttg
//
LOCUS       DAORVN010000200         3037 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_28729_length_3037_cov_3.302146, whole genome shotgun sequence.
ACCESSION   DAORVN010000200 DAORVN010000000
VERSION     DAORVN010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3037
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28729_length_3037_cov_3.302146"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..724
                     /locus_tag="PKV23_05065"
     CDS             <1..724
                     /locus_tag="PKV23_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011582683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQX84839.1"
                     /translation="ISGEFAYGVAVGLMIVAICCVVLIGRVPQANHAQETSLATLLAG
                     FKFIRSEKIVLGAISLDLFAVLLGGAVALLPVYARDILDVGPWGLGLLRAGPGIGAIL
                     MAIWLSKYPIKDHAGVILFVFVAGFGFCTIVFGLSKSVPLSIIALALMGACDMVSVYV
                     RETLMQLWTPDAVRGRVNAVNRVFIGASNELGEFRAGVVAAWIGAVAAVAIGGAGTMA
                     VAFFWSRWFPELREARTLSGKG"
     gene            761..1576
                     /locus_tag="PKV23_05070"
     CDS             761..1576
                     /locus_tag="PKV23_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012111502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HQX84840.1"
                     /translation="MAGTDKTQNQVLGPAPLVVLAHPQLGENIGTAARAMANFGLHKL
                     RLVAPRDGWPNEKAVTASSGAHWIVEGAKVFDDLPSALIDMNYVYATTARPRDMIKEV
                     ITPEQAGHDMRARVARGEKVAILFGRERWGLSNEEVSLADVIVTAPVNPAFASINIAQ
                     AVLLMGYEWFKEHAMSLGQQTPELAALDGPGLQTPDTRLATKEELFGFFDHLERELDD
                     AGFFKTEKKKPTMRLNLRNMFVRAELTEQEIRSLRGMVSSLTRTHLRGKEPKA"
     gene            1573..2391
                     /gene="murI"
                     /locus_tag="PKV23_05075"
     CDS             1573..2391
                     /gene="murI"
                     /locus_tag="PKV23_05075"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004448599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HQX84841.1"
                     /translation="MTTPRILLFDSGMGGLTVATCVRAQLPNAELIYAADNAGFPYGA
                     WKEEALIARIVHVMGALIEKARPDVVVIACNTASTLALAALREKYAVPFVGTVPAIKP
                     AAAQTKSNIIGVLATPGTVSREYTHTLIHTYAFHCKVILHGAKRLAEIAEQKLKGHAP
                     DLDELRDEIASVFRKRDGKRTDVVVLACTHYPLVIDEMKLVAPWEVSYLDPAPAIARR
                     VSDVVADLKLAGPQMGVPEPDTVLLTSDRGSASESLAAYAAMGFPRHEFVDLPV"
     gene            2538..>3037
                     /gene="rpsD"
                     /locus_tag="PKV23_05080"
     CDS             2538..>3037
                     /gene="rpsD"
                     /locus_tag="PKV23_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011314595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HQX84842.1"
                     /translation="MTKRQSAKHKIDRRMGENIWGRPKSPVNKREYGPGQHGQRRKGK
                     MSDFGIQLKAKQKLKGYYGNISERQFSKVYEEASRMKGDTSENLIGLLERRLDAIVYR
                     AKFVPTMFAARQFVSHGHVKVNGRRVTIASMRIKAGDALELTAKSKELPIVLEAVQLA
                     ERDVPD"
BASE COUNT          570 a          855 c          980 g          632 t
ORIGIN      
        1 aatctccggt gagtttgcct acggtgttgc tgtcggcctc atgattgtgg cgatctgctg
       61 cgtggtgctg atcggccggg tgccccaggc aaaccatgcc caggagacaa gcctggcgac
      121 gctgctggcg ggcttcaaat tcatcaggtc cgagaaaatc gtgctggggg cgatttcgct
      181 cgatcttttt gctgttttgc ttggcggggc cgtggccctg ctgcccgttt atgcccgcga
      241 tatcctggac gtcggcccct gggggcttgg cctgctgcgg gccggacccg ggatcggcgc
      301 gatcctcatg gccatctggc tttcgaagta tccgatcaag gaccacgccg gggtgatcct
      361 gtttgtgttc gtggccggtt ttggcttctg caccatcgtg ttcgggcttt ccaaatcggt
      421 tccgctgtcg attattgccc ttgccctcat gggggcgtgt gacatggtca gcgtctatgt
      481 gcgtgaaacc ctcatgcaat tatggacgcc ggatgcggtg cggggccggg tgaatgcggt
      541 caaccgggtg ttcatcgggg cctccaatga actgggcgaa ttccgcgccg gggttgtggc
      601 ggcctggatc ggggcggtgg cggcggtggc catcggcgga gcggggacca tggcggtggc
      661 cttcttctgg tcgcgctggt tcccggaact gcgcgaagcg cggacgctaa gcgggaaagg
      721 ctagcgtcag gcactcaact tcgctaatcc cccggggacc atggcaggca ccgataaaac
      781 gcagaatcag gttttgggcc cggcacccct tgtggtgctg gcgcatccgc agcttggcga
      841 gaatatcggc acggcggccc gggccatggc gaatttcggc ctgcataagc tcaggcttgt
      901 ggcgccgcgc gatggctggc ccaatgagaa ggcggtgacg gcttcttccg gagcgcactg
      961 gattgtggaa ggcgcgaaag tcttcgatga tttgccttcg gcccttatcg acatgaacta
     1021 cgtctatgcc accacggcgc ggccgcgcga catgatcaag gaagtgatca cgccagagca
     1081 agcgggccat gacatgcggg cgcgcgtggc gcgtggcgaa aaagttgcca tcctctttgg
     1141 gcgtgagcgc tggggcctga gtaacgagga agtttcgctg gccgatgtca ttgtaaccgc
     1201 tccggtgaac ccggcctttg cttcgatcaa tatcgcgcag gcggttttgc tcatgggcta
     1261 tgaatggttc aaggaacatg cgatgagcct tgggcagcaa acgccggaac tggcagcgct
     1321 tgatggtccc ggcctgcaaa cgcccgacac gcgccttgcc accaaggaag aacttttcgg
     1381 tttctttgac catctcgagc gggagctgga tgacgccggg ttcttcaaaa ccgaaaaaaa
     1441 gaagccgacc atgcgtttga acctgcgcaa tatgtttgtg cgggcggaat tgacggagca
     1501 ggaaatccgt tcgctccgtg gcatggtttc gtcgctgacg cggacgcatt tgcggggcaa
     1561 ggaaccaaag gcatgaccac gccccgcatt ctcctgtttg attccggcat gggtggactg
     1621 accgttgcta cctgtgtgcg ggcgcagttg ccaaacgctg aattgattta cgcggcggac
     1681 aacgccggct ttccctatgg tgcctggaaa gaagaggcgc ttattgcccg gattgttcat
     1741 gtcatgggtg cgctcatcga gaaagccagg ccggatgtgg tggtgattgc ctgcaacacg
     1801 gcttccacac tcgcccttgc ggcgctgcgg gaaaaatatg ccgttccctt tgtgggcacg
     1861 gttccggcga tcaagccggc ggcggcgcaa accaagtcca acatcatcgg cgttctggcc
     1921 acgcccggca cggtgagccg cgaatacact cacacgctca ttcataccta tgccttccac
     1981 tgcaaggtga tcctgcatgg ggccaagcgg ctggctgaaa tcgccgaaca gaaactcaag
     2041 ggccatgccc ccgatctgga tgaactgcgc gatgagattg cctcggtgtt tcgcaagcgt
     2101 gacggcaagc ggaccgacgt ggtggtgctg gcctgcacgc attatccgtt ggtgatcgac
     2161 gagatgaagc tggtggcgcc ctgggaggtt agctatctcg acccggctcc agccattgcc
     2221 cgccgcgtaa gtgacgttgt ggctgacctg aaactggcgg ggccgcaaat gggcgttccc
     2281 gagccggata cggtgctttt aacctccgat aggggcagcg cttcggaatc gcttgccgcc
     2341 tatgccgcta tgggtttccc gaggcatgaa ttcgtggatt tgccggtttg acaggcggct
     2401 gctgctagtc tagaagcccc tcactgacgg ggccagaagc cccgttttgc ttgcccgtga
     2461 acccttaagg gttctgtcgg ccgggagacc ggaagaggag ggtgggttcc aacttgaatg
     2521 gttatggaga accgtaaatg acaaagcgtc agtccgctaa acataagatc gaccgccgca
     2581 tgggcgaaaa catctggggc cgtccgaaga gcccggtgaa caagcgtgaa tatggccccg
     2641 gccagcacgg ccagcgccgc aagggcaaga tgtcggactt cggcatccag ctcaaggcca
     2701 agcagaagct taaaggttat tacggcaaca tttccgagcg ccagttctcg aaggtttacg
     2761 aagaggcttc gcgcatgaag ggtgacacct cggaaaacct gatcggcctt ctcgagcgcc
     2821 gccttgacgc catcgtctac cgcgccaagt tcgtgccaac catgtttgcg gcgcgccagt
     2881 tcgtgagcca cggccatgtg aaggtgaacg gccggcgcgt gaccatcgct tccatgcgca
     2941 ttaaggccgg cgacgccctg gagctcacgg caaagtccaa ggagcttccg atcgtgcttg
     3001 aagccgtgca attggccgag cgcgatgttc cggatta
//
LOCUS       DAORVN010000201         3031 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_28839_length_3031_cov_3.637433, whole genome shotgun sequence.
ACCESSION   DAORVN010000201 DAORVN010000000
VERSION     DAORVN010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3031
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28839_length_3031_cov_3.637433"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..595)
                     /gene="hemH"
                     /locus_tag="PKV23_05085"
     CDS             complement(<1..595)
                     /gene="hemH"
                     /locus_tag="PKV23_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012317738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HQX84843.1"
                     /translation="MIRDSALAFRPPDHPKVKNGRIGVLLMNLGTPEATTYWPMRRYL
                     KEFLSDRRVIETNPVLWWFILNGIILTLRPQKSGKAYEKIWNKALNESPLKTITRGQS
                     DKLQAALKDRPEIIVDWAMRYGQPPVATRIEALQAQGCDRILFVPLYPQYSAATTATA
                     LDKCFDALKAMRWQPAIRSLPPYFDHPAYIDALAKSLK"
     gene            715..2565
                     /locus_tag="PKV23_05090"
     CDS             715..2565
                     /locus_tag="PKV23_05090"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HQX84844.1"
                     /translation="MDSVETGRSPFFSQLQKEGQRAADLVFTDLDTTATTSNIEPGVF
                     RTSQVNTIYGTLPLFVLAHIVVVPAFASAIAHQLQTAMTTWWLTLSIVPVALLVILWC
                     HLSRRNSPGASVAEIRWIELSGILFGLSWAMFPAAFFAPADADLRILIVAGTLAASAM
                     GTFALSRVPAAAIVFCALITGSLSFSVSQLGGPTALTFTVFTAAYGLILAGMVLNQHR
                     DHLQKALDAQEMHHQKDIIALLLNDFEQGTSDWLFECDANGSLIYGSARLGEIVGKSP
                     QDIKGLTLLDAAGADATHVGWVEFYETMQAREPVVARHLEVDLAGKTCHWQISARPLF
                     SENGVFRGYRGVGRDITAEWETDQKLIAAKEAAEAASAAKSQFLAVMSHELRTPLNSI
                     VGFSEILSSRSEETLQPEVRLDYAKTILNSSKHLQTLITDILDATRIENGTIALIEQD
                     IDAAELVEIAVKMCRDQAEKADVTLVAKLTDGIEIRADITRIKQVILNLLTNAIKFSL
                     TGGVVNIELLRTHNDRLALVVRDGGIGIKSEDLGRIFEPFVQAEDGMARRFSGIGLGL
                     SIARKIARLHGGDVTLESQFGVGTTARLELPRSRVTWPRKSANPAPGVAA"
     gene            2590..>3031
                     /locus_tag="PKV23_05095"
     CDS             2590..>3031
                     /locus_tag="PKV23_05095"
                     /inference="COORDINATES: protein motif:HMM:NF012703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HQX84845.1"
                     /translation="MTRIPRIPPFIGTTRSRSPAGFHAAVFGAPHGTPYPGIDNRIHA
                     TAPDAFRRALGEDGEWLDHWDFDIGGPLLADGTCRIADLGNLPTKSKDGKGNRKLIEA
                     QTRKILAAGAVPLIFGGDDSIPIPFIAGFSGSSPLTILQIDAHID"
BASE COUNT          598 a          941 c          838 g          654 t
ORIGIN      
        1 gcttcaggct cttagccagc gcgtcaatat aggcagggtg gtcgaaatag ggcggcaggc
       61 tgcggatggc gggttgccag cgcatggcct tcagcgcatc gaaacacttg tccagcgccg
      121 tcgccgtggt cgcggcactg tattgcggat agagcggcac aaacaggatc cggtcgcagc
      181 cctgtgcctg gagggcttcg atgcgggttg ccaccggcgg ctggccataa cgcatggccc
      241 agtcgacaat gatttccggc cggtccttga gcgccgcctg aagcttgtcg ctttgcccgc
      301 gcgtaatggt cttgagcgga gattcattaa gggccttgtt ccagattttt tcgtaagctt
      361 tgccgctctt ctgcggacgg agcgtcagga tgatcccgtt caggatgaac caccataaga
      421 ccgggtttgt ctcgatgacc cgccggtccg acaggaattc cttgagatag cgccgcatcg
      481 gccaataggt cgtggcttcc ggcgttccca aattcatcag caggacgcca attctgccat
      541 tcttaacttt cgggtgatct ggcggccgga aggccagcgc tgaatcgcgt atcatgggtc
      601 tcggccaaaa atcgacgcag gggcgcaatc aaccctgccg gatgacgtta acatattgca
      661 aactctgatt gaataacgtg cgttagcagg caatcgagca aggctcatgt aactatggat
      721 agtgtggaaa ccgggcgaag tccattcttc tcccagctcc agaaggaagg tcagcgggcc
      781 gctgatcttg tttttaccga tcttgacaca actgcaacga cctcgaacat cgagcccggc
      841 gttttccgga cctcgcaagt aaacaccatt tacggcactc tgccgctatt tgtcctggct
      901 cacattgttg tggtccccgc ttttgcctcg gccatagcgc accagcttca aactgcaatg
      961 accacttggt ggctcaccct ttccatcgtg cccgtggcac ttcttgtcat tctctggtgc
     1021 catctttccc gaagaaattc gcctggagcc agcgtcgcgg agattcgctg gattgagctg
     1081 tccggcatcc tttttgggct ttcctgggct atgttcccgg ccgctttttt tgcccccgct
     1141 gatgccgatt tgcgtatcct gattgttgct ggaacgctgg ctgcctcggc aatgggcact
     1201 ttcgccctgt cacgcgttcc cgctgccgca attgtctttt gcgccctgat cactggctcg
     1261 ctttcattct cggtttcaca actcggcggc ccgaccgccc tcaccttcac tgttttcacg
     1321 gctgcctatg gcctcattct cgccggcatg gttctcaatc agcatcgcga ccatttgcag
     1381 aaagctctcg atgcccagga aatgcaccat cagaaagaca tcatagcatt gctgctcaat
     1441 gattttgagc agggcaccag cgactggctg tttgagtgcg acgccaatgg ttctctcatt
     1501 tacggctctg ccaggctcgg tgaaattgtc ggcaaatcgc cgcaagacat caagggcctg
     1561 acgctgctgg atgccgctgg cgccgatgcg acgcatgtcg gctgggtaga gttctacgag
     1621 acaatgcagg caagagagcc ggttgtggcc cgccaccttg aggtcgacct ggcgggaaag
     1681 acctgccact ggcaaataag cgcccggccg ctgttctccg agaatggtgt cttcaggggc
     1741 taccgcggtg taggccgtga catcaccgcg gaatgggaaa cggaccagaa gctaattgcc
     1801 gccaaggaag ctgccgaggc cgcgagtgcc gcgaaatcgc aattcctggc cgtcatgagc
     1861 cacgaactgc gcacccctct caactccatt gtgggatttt ctgaaatcct gtcctcgcgc
     1921 agcgaggaaa cactacagcc cgaagtccgg ctcgattacg caaaaaccat tctgaacagc
     1981 agcaagcacc tgcagaccct gatcaccgat attctggatg cgacccgcat cgaaaatggc
     2041 acgattgccc tcatcgaaca ggacatcgac gccgccgaac ttgtcgaaat cgccgtgaag
     2101 atgtgccgcg accaggccga aaaggccgat gtcacgctgg tcgcgaaact caccgacggt
     2161 attgagatac gcgccgacat tacccgcatc aaacaggtca ttctcaacct gctgaccaat
     2221 gccatcaagt tttccctgac cggcggggtc gtgaacattg aactgctgcg cacgcacaac
     2281 gaccggcttg cccttgttgt tcgcgacggc ggcattggca tcaagtcgga agatctcggc
     2341 cgtatttttg aacccttcgt gcaggccgag gacggcatgg cccggcgttt ttccgggatc
     2401 ggcctcggcc tgtccatcgc ccggaaaatt gcccgcctcc acggtggcga cgtcaccctc
     2461 gaaagccagt tcggggttgg cacgacggcg cggttagaat tgccgcggtc acgcgtcacc
     2521 tggccccgca agtcagccaa tccggcgccg ggtgttgcgg cctaggaaga ttcacgccat
     2581 cctgcgtcga tgacaaggat accccgaatc ccgcccttca tcggaaccac gcgttcacgc
     2641 tcgccggcgg gttttcatgc cgcggttttt ggcgcacccc acggcacgcc ctatccgggg
     2701 attgacaacc gcatccatgc cacggcgccc gacgccttcc gccgcgccct tggcgaagat
     2761 ggcgaatggc tggaccattg ggattttgac ataggcggcc cgctgctggc cgacggcacc
     2821 tgccgcattg ccgatctcgg caacctcccg accaagagca aggacggcaa gggcaaccgc
     2881 aaactcattg aagcgcagac ccggaaaatt cttgccgccg gcgccgtgcc gcttatcttc
     2941 ggcggcgatg attcaatccc catacctttc attgccggct tttccggaag ctcgccgctc
     3001 accatcctgc agatcgacgc ccatatcgac t
//
LOCUS       DAORVN010000202         3030 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_28862_length_3030_cov_3.128067, whole genome shotgun sequence.
ACCESSION   DAORVN010000202 DAORVN010000000
VERSION     DAORVN010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3030
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28862_length_3030_cov_3.128067"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..652
                     /gene="hisG"
                     /locus_tag="PKV23_05100"
     CDS             <1..652
                     /gene="hisG"
                     /locus_tag="PKV23_05100"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006204020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HQX84846.1"
                     /translation="LMEQAAAVLEKAGLSIMRSGAERGYRGKLQGMDGIEVAFLSAAE
                     IAQNLREGKIDFGVTGADLLWEKSAPGENLVSVLAPLRFGYADVAVAVPECWLDVASM
                     ADLDEVSESFYLRHGRRLRIATKYLNLTRRFFAERGVTGYRIVESLGATEGAPAAGTA
                     EVIVDITTTGSTLAANHLKVLDDGIILCSSAVLAASPAALGDPRAGLLQNRLKAAQ"
     gene            998..2206
                     /locus_tag="PKV23_05105"
     CDS             998..2206
                     /locus_tag="PKV23_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HQX84847.1"
                     /translation="MLKSMRLSAVACASAVFLVAGSNARAADCGNSGSGFSGFIKEFH
                     REAQAQGIGRQALSVLDGITYDPGIIKKDRAQNIFSQSFLEFQARMITDYRIKRGAEL
                     VQKHKATFARVEKQYGVPAPVIVAFWALETDFGANIGDLSTVRSLATLAWDCRRPEKF
                     RPQLMAALELIDRGDLHPDEMRGAWAGEIGQTQFLPYDYNESAVDFDGDGRRNLRASI
                     PDVLASSANLLRKHGWQPGQPWMQEVRVPRDMPWEEADIAIQHPRSQWAKWGVKMANG
                     KPMKADSFPASLLLPMGKDGPAFLVYENFTTAYLKWNESLVYSTTASYLATRIAGAPK
                     VNRGNAEVTHLTYQQIVALQKKLQARGHDVGKLDGKIGAGTRAAVKTMQIELGMPADS
                     YPTLELLQQL"
     gene            complement(2216..>3030)
                     /locus_tag="PKV23_05110"
     CDS             complement(2216..>3030)
                     /locus_tag="PKV23_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007601008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQX84848.1"
                     /translation="TFLSGRPFSDGDIRSGSSVCVIGQTIKTDLFGQADPLGQRLRLG
                     NVSCEVIGAVEGKGQSAFGTDQDSVVFMPLRAFQRRIAGSQRIATIYISVASADAVDA
                     VKSSTEDILREQRRVGANEEDDFSVRDMSQIVSTLSSTTTLMTGLLSAVAAVSLLVGG
                     IGIMNIMLVSVTERTREIGIRLAVGALEIQVLTQFLVEAIVLAVFGGIIGIVLGLSLA
                     GWAAYAMAIPFTTNPSIIALAFLFSGLIGVVFGYFPARRAARLDPIEALRHE"
BASE COUNT          621 a          925 c          911 g          573 t
ORIGIN      
        1 ccttatggaa caggcagcgg cggttttgga gaaagcaggc ttaagcatca tgcgttccgg
       61 cgcggagcgt ggctaccgcg gcaagttgca aggcatggac ggcatcgaag tcgcttttct
      121 ttcggccgcc gaaattgccc agaacctgcg cgagggaaag attgatttcg gcgtcaccgg
      181 cgcggacctt ctctgggaga aatccgcacc gggagaaaat cttgtgtcgg ttctggcgcc
      241 gctgcgattc ggctatgcgg atgttgccgt ggccgtgccg gaatgctggc tcgatgtggc
      301 ctccatggcc gatctcgatg aagtctccga aagcttttat ctgcgccacg gccgcaggct
      361 caggatagcc acgaaatatc ttaacctcac ccggaggttc tttgccgaaa ggggcgtgac
      421 cggctaccgc atcgtcgaga gcctgggggc caccgaaggg gcgcccgcgg ccggaaccgc
      481 ggaggtgatc gtggacatca ccaccaccgg ttcaaccctt gccgccaatc atctcaaggt
      541 cctcgatgac ggcataatcc tgtgttcctc ggcggtgctg gcggcaagcc ctgccgcact
      601 tggcgatccc cgcgccggcc ttctgcagaa ccgcctcaaa gcggctcaat gattgccccg
      661 ccaccccgaa ccctccctga atttgcaggg ggccctgtga aaaatttgcc ggggaacggt
      721 tcaattcaaa gagaaactgg ctatattgcg ccccattcag ggctagggcg ggaccatgca
      781 cccgagttcc gcacttgcga gagtctggta tcgcccccgg ttcagatttt cgtgacggag
      841 tgtgaattcg ggaggatggt tctccagcct gagcgagggg tcgtactttg tacggcccct
      901 cgtgatcttg ccagcccgcc gcatcacagc cataaaacag ggccttgatt ttgactcaag
      961 cgatgaataa tcaggcaaaa aaccagggag tctcggaatg ttgaaatcga tgcgtttgag
     1021 cgccgtggcc tgtgccagtg cggtatttct agtggcaggt tctaacgccc gggctgccga
     1081 ttgcgggaac tcaggcagcg gcttttccgg cttcatcaag gaatttcacc gggaagccca
     1141 agcccagggc attggccgcc aggcgctttc cgtgctcgat ggcattacct acgatcccgg
     1201 catcatcaag aaagaccggg cgcaaaacat cttctcccag agctttctcg agttccaggc
     1261 ccgcatgatc actgattacc gcatcaagcg cggggcggaa ttggtccaga aacacaaggc
     1321 cacctttgcc cgcgtcgaaa agcagtatgg cgtacccgct ccggtgattg tcgccttctg
     1381 ggctttggaa accgatttcg gcgccaacat cggcgatctc tcgacagtta ggtcgcttgc
     1441 cacgctcgcc tgggattgcc ggcggccgga aaaattccgc ccgcaactga tggcagcgct
     1501 tgaactcatc gaccgtggcg atttgcaccc cgacgaaatg cgcggcgcct gggccggaga
     1561 aatcggccag acgcagttcc tcccctatga ctacaatgaa agtgccgtcg acttcgatgg
     1621 cgatggccgg cgtaatttgc gcgcaagcat tcccgatgtg ctggcctctt cggccaatct
     1681 cctgcgcaag catggctggc agccgggcca gccctggatg caggaggtgc gcgtgccgcg
     1741 tgacatgccg tgggaggaag ccgatatcgc catccagcat ccccgctcgc agtgggccaa
     1801 gtggggcgtg aagatggcca acggcaagcc catgaaggcc gacagcttcc ccgcgtccct
     1861 gctgctgccc atgggcaagg atggcccggc cttcctcgtc tatgagaact tcactaccgc
     1921 ctatctcaag tggaatgagt cgctcgtcta ttccactacc gcctcctatc tcgctacccg
     1981 cattgccggt gcgccgaagg tgaaccgtgg aaatgccgag gtgacgcacc tcacctatca
     2041 gcaaatcgtg gcgctccaga aaaagctgca agcccgtggt catgacgtgg gcaagctgga
     2101 cgggaagatt ggcgcgggaa cccgcgctgc cgtcaagaca atgcagatcg agctgggaat
     2161 gcccgcggat tcctacccaa ccctggagct gctccagcag ctctgagcgg cgccgctact
     2221 cgtggcggag cgcctcgatc ggatcgaggc gcgccgcgcg ccttgccggg aaatagccga
     2281 acacgacgcc gatcaggccg gaaaacaaaa aggccagggc gatgatgctg gggttggtcg
     2341 taaatggaat ggccatggcg taggcggccc atcccgccag ggaaaggccg aggacgattc
     2401 cgatgatgcc gccaaagacc gcaaggacaa tggcctcgac gaggaactgg gtcaaaacct
     2461 ggatttccag ggcgccgacg gcaaggcgga tcccaatctc gcgggtgcgc tcggtgaccg
     2521 agacaagcat gatattcatg atgccaatcc cgcccacaag caggctgaca gccgcaacgg
     2581 cgctcagcag ccccgtcatc agtgtcgtcg tcgaactcag tgtactgacg atttgcgaca
     2641 tgtcgcgcac cgaaaaatca tcctcctcgt tggccccgac gcggcgctgt tcgcgcagga
     2701 tgtcttccgt cgacgatttc actgcgtcga ccgcatcggc ggacgccact gaaatataga
     2761 ttgtcgcgat gcgctggctt ccggcgatcc ggcgctgaaa ggcgcggagc ggcatgaata
     2821 caacgctgtc ctgatcggtg ccgaacgccg attgcccctt gccttccacg gcgccgatga
     2881 cttcgcacga aacattgcca agtctcaggc gttggcccag tggatcggcc tgcccgaaga
     2941 ggtccgtttt tattgtctgg ccgatcacgc agacggagct gcctgaacgg atatcgccgt
     3001 ccgaaaaagg ccggcccgat aaaaaggtcc
//
LOCUS       DAORVN010000203         3028 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_28897_length_3028_cov_3.436596, whole genome shotgun sequence.
ACCESSION   DAORVN010000203 DAORVN010000000
VERSION     DAORVN010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3028
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28897_length_3028_cov_3.436596"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(75..1088)
                     /gene="trpD"
                     /locus_tag="PKV23_05115"
     CDS             complement(75..1088)
                     /gene="trpD"
                     /locus_tag="PKV23_05115"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009028659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HQX84849.1"
                     /translation="MSDLKPIIAKAADGKPLTRDEARTAFGVMMSGDATPSQIAGLLM
                     ALRVRGETVDEITGAVEIMREKMIRVSAPANAIDIVGTGGDASGSYNISTCAAIVAAG
                     AGLKVAKHGNRALSSKSGAADVLMALGVKIDLLPEKISECIAEAGVGFMFAPAHHSSM
                     KYVGPTRVELGTRTIFNLLGPLSNPAGVKKQVTGVFAKAWVEPLAHVLKNLGSEACWI
                     CHGEGGIDEIVPTGTNWISELKNGTITSFEITPEAAGLKRSSFANLKGGEASHNAAAL
                     RGVLQGEKSAFRDAVVMTTGAALLVDGRVKAYAEGVRFAEQAIDSGAALRAHDKLVKV
                     SNS"
     gene            complement(1088..1660)
                     /locus_tag="PKV23_05120"
     CDS             complement(1088..1660)
                     /locus_tag="PKV23_05120"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013418062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HQX84850.1"
                     /translation="MIILIDNYDSFTYNLFHYLGDLGVETVVHRNDKISVGQVISAKP
                     QAIVLSPGPKTPNEAGICLELIEKASDTIPIFGVCLGLQAIGQAFGGKVVRAPKLMHG
                     KLSTVHHGGKGVFKDIPNDFAATRYHSLVVEKTGLPDCFEITAETDGLVMGLQHKTRN
                     VHGVQFHPESIASQHGHALLKNFLGLAKVR"
     gene            complement(1657..>3028)
                     /gene="trpE"
                     /locus_tag="PKV23_05125"
     CDS             complement(1657..>3028)
                     /gene="trpE"
                     /locus_tag="PKV23_05125"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007561517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HQX84851.1"
                     /translation="ESVTGGEVRGRYSIIGMNPDMIWKVAGSEASINRKALHNPDGPF
                     EPARLAPLESLRALLAESRITLGSDAPPMAAGVFGYIGYDMVRLMEDLPEPNPDTLGL
                     PDGMMIRPTVMAIFDSVRDEVTVTSPVYFEKGVSTKAAYARAQERISEVVDALDRPLD
                     HLSSQDIETPPIMAAASNTTPQEYAGMIARAKDYITAGDIFQVVLSQRFEADFALPPF
                     ALYRSLRRVNPSPFLYYLDFDRFAVIGSSPEILVRVRDGKVSIRPIAGTRKRGDTPSE
                     DRALAAELLADPKERAEHLMLLDLGRNDVGRVAEIGSIRVTDQFIIENYSHVMHIVSN
                     VEGTLSKKYDVIDALCAGFPAGTVSGAPKVRAMEIIDELEKHKRGVYGGCVGYFSADG
                     EMDTCIVLRTAIVKDGKMYVQAGAGIVADSVPENEHQECVNKAKALFRAADEAVRFAG
                     RAGRAQ"
BASE COUNT          598 a          865 c          929 g          636 t
ORIGIN      
        1 atgcggcaac gcgtgccggg ccttggccac ttcttcgcgc ttgtaggccg caatcttgtc
       61 aaggatcgtg gccatcagga gtttgaaacc ttgaccagct tgtcgtgggc tctgagtgcc
      121 gcaccactgt caatggcttg ttcggcgaag cgaacgcctt cagcgtatgc cttgaccctg
      181 ccgtcaacaa gaagagcggc gccggtggtc atgacgacgg cgtcacggaa ggcagatttt
      241 tcaccctgaa ggacgccgcg cagggcggca gcgttgtggc tggcttcgcc acctttaagg
      301 ttcgcaaagc tcgaacgctt gaggcctgcc gcttccggcg tgatttcaaa gctggtgatg
      361 gtgccgttct tcaactcgga aatccagttg gttccggttg gcacaatttc gtcaatgccg
      421 ccttcgccat ggcagatcca gcaggcttca ctacccaggt ttttcaggac atgggcaagc
      481 ggttccaccc aggcttttgc aaacacgccg gtgacctgtt tctttactcc ggctggattg
      541 gacagggggc cgagcagatt gaaaatggtg cgagttccga gttcaacgcg ggttggcccc
      601 acatatttca tggaagaatg gtgggccggg gcgaacatga aaccgacgcc ggcctcggca
      661 atgcactcac tgatcttttc agggagaaga tcaatcttca ctcccagggc catgagaacg
      721 tccgccgcac ctgactttga ggagagggcg cgattgccgt gcttggccac tttcaggccg
      781 gcgcctgcgg cgacaatggc ggcgcaagtc gagatgttat aggagccgga ggcatccccg
      841 ccggttccaa caatatcgat ggcgttggcg ggggcgctaa cgcggatcat tttttcgcgc
      901 atgatctcca ccgcgccggt gatctcgtca acggtttcgc cacgcaccct gagcgccatt
      961 agaaggccgg caatctgtga tggcgtggca tcgcccgaca tcatgacgcc gaatgcggtt
     1021 cgggcttcgt cgcgggtcag cggcttgccg tcggccgcct tggcgatgat tggtttaaga
     1081 tctgacatca gcgcaccttt gccaagccga ggaaattctt gagaagcgca tggccatgct
     1141 gcgaggctat gctttcgggg tggaattgca cgccatgcac atttcgggtt ttatgctgga
     1201 gacccatgac caggccatcg gtctcggcgg tgatttcgaa acagtcggga agaccagttt
     1261 tttcaacgac aagggaatga tagcgggtgg ccgcaaaatc gttgggtata tctttgaaaa
     1321 cgcccttgcc tccgtgatgc acggttgaga gcttgccatg catcaatttc ggggcgcgga
     1381 ccaccttgcc gccgaaggcc tggccgatgg cctggaggcc aagacagaca ccgaagatgg
     1441 gaatggtgtc cgaggccttt tcaatcaatt ccaggcaaat tccggcctca ttgggggttt
     1501 tgggtcctgg cgaaagcaca atggcctggg gcttggccga gatgacctgg cccacgctga
     1561 tcttgtcatt gcggtggacc acggtttcaa cgcccaaatc gcccaggtaa tggaacaggt
     1621 tgtaggtgaa gctgtcgtag ttatcgatga ggatgatcat tgggccctcc cggcgcggcc
     1681 cgcaaagcga acggcctcat cggcggcgcg gaacagggcc ttggccttgt tcacgcattc
     1741 ctgatgttca ttttcgggca cactgtcggc cacgatgccg gcgccggcct ggacatacat
     1801 cttgccgtcc ttgacgatgg cggtgcgcag cacaatgcag gtgtccattt caccgtcggc
     1861 ggagaaatag ccaacgcagc cgccgtaaac accgcgtttg tgcttttcca gttcgtcgat
     1921 gatctccatg gcacgcacct tgggcgcgcc ggaaaccgtc ccagccggaa agccggcgca
     1981 caaagcgtca atcacgtcgt attttttgga cagagtgcct tcgacattcg aaacgatgtg
     2041 catgacgtgg ctgtagtttt cgattatgaa ctggtcggtc accctgatgg agccgatctc
     2101 cgctacccgg cctacgtcat tgcggccgag atcgagcagc atcaggtgtt cggcccgttc
     2161 cttgggatcg gccagcagtt ccgccgccag cgcccgatct tcagacggcg tatcgccgcg
     2221 ctttcgggtt ccggcgatgg gcctgatcga gaccttgcca tcacgtacgc gcacgagaat
     2281 ttccggactg gatcctatga ccgcaaaccg gtcgaagtcg aggtaataga ggaagggtga
     2341 ggggttcacc cggcgcagtg aacgataaag cgcaaaaggc ggcaaggcga agtctgcctc
     2401 gaagcgttgc gacagaacca cctggaaaat gtcgccggcg gtaatgtagt ccttggccct
     2461 ggcgatcatg ccggcatatt cctgcggcgt ggtattcgac gcagcggcca tgatcggcgg
     2521 ggtttcaatg tcctgggagc tcagatggtc cagagggcgg tccaatgcat cgacaacctc
     2581 tgaaatccgc tcctgggccc gcgcataggc ggccttcgtc gagacgccct tctcgaaata
     2641 gaccggtgag gtgacggtca cctcgtcgcg gacactgtca aaaattgcca tgaccgtcgg
     2701 gcggatcatc atgccgtcgg gcaggccaag cgtatcggga ttgggttcag gcaagtcctc
     2761 catcaggcga accatatcat agccgatgta gccgaagacg cctgccgcca tgggcggcgc
     2821 atcgctacca agagtaatcc ggctttcggc aagcagggcg cggagcgatt caagcggggc
     2881 cagtcgggcc ggctcaaagg gcccatcagg gttgtgcaga gccttgcggt tgatcgaggc
     2941 ctcgctgcct gcgaccttcc agatcatatc gggattcatc ccgatgatgg aatagcgccc
     3001 gcgcacctcg ccgccggtca cgctttcc
//
LOCUS       DAORVN010000204         3025 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_28958_length_3025_cov_2.202357, whole genome shotgun sequence.
ACCESSION   DAORVN010000204 DAORVN010000000
VERSION     DAORVN010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3025
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28958_length_3025_cov_2.202357"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="PKV23_05130"
     CDS             complement(<1..252)
                     /locus_tag="PKV23_05130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HQX84852.1"
                     /translation="MNIRARLMFLVMLATLLPAALIGLRFIHDRVEHIEVAKRNLEQA
                     ADRLALRIDSSILGATQLLYGLARAKDLETNDRMACSSFL"
     gene            complement(462..>3025)
                     /gene="carB"
                     /locus_tag="PKV23_05135"
     CDS             complement(462..>3025)
                     /gene="carB"
                     /locus_tag="PKV23_05135"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009337237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HQX84853.1"
                     /translation="IIVCSIENVDPMGVHTGDSITVAPALTLTDKEYQIMRNASIAVL
                     REIGVETGGSNVQFGVNPADGRLVVIEMNPRVSRSSALASKATGFPIAKIAAKLAVGY
                     TLDELANDITGGATPAAFEPTIDYVVTKIPRFAFEKFPGSQAILSTAMKSVGEVMAIG
                     RTFQESVQKALRGLETGLSGFDEIEIPGLGEGDDKNAIRAALGTPTPDRLLIIAQAFR
                     HGATVEQIFASCKYEPWFLRQIEELVQTEAKIRANGLPNDAVNFQKLKAMGFSDRRLA
                     ALVGVDEDKVRKHRHKLNVRPVFKRIDTCAAEFASPTAYMYSTYETGFIGRNGNEAKP
                     SDRQKVIILGGGPNRIGQGIEFDYCCCHACFALHDAGYESIMVNCNPETVSTDYDTSD
                     RLYFESLTIEDVLEIIHVEQSNGALKGVIVQFGGQTPLKLAGKLEELGVPILGTTPDA
                     IDLAEDRDRFSRLLKKLKLRQPENGIARSAAEAKKIARKIGFPVVIRPSYVLGGRAME
                     IVRDDDHLQRYITEAVVVSGDSPVLIDSYLSNAVEVDVDAICDGREVFIAGVMEHIEE
                     AGIHSGDSACSLPPYSLKAPVIAEMEAQARKMALALNVVGLMNVQFAIKDDLVYVLEV
                     NPRASRTVPFVAKVIGIPVAKIAARVMAGETLASFKLVNRKLAHVAVKEAVFPFNRFP
                     GVDVLLGPEMRSTGEVMGLDKTYDMAFAKSQLGAGMRIPQSGTVFVSVNDPDKSKILP
                     AIRKLVDLGFVIKATGGTQQFFESKGIPSSKINKVLEGRPHVVDAIKNGEIQLVLNTT
                     ESKASESDSKSIRQTAMLQKVPYYTTLPGILAATKAIAAQKADTIRVRPLQDYFS"
BASE COUNT          587 a          785 c          992 g          661 t
ORIGIN      
        1 cagaaaggac gaacaggcca tcctgtcgtt tgtttcgagg tccttggcgc gggccaaccc
       61 ataaaggagc tgcgtcgccc ccaatatgct gctgtcaatt ctcaacgcga gcctatcggc
      121 agcctgctca agattgcgct ttgccacttc gatgtgttcg acgcggtcat ggataaaacg
      181 gagaccgatg agggccgcag gcagcaaggt cgcgagcata accagaaaca tcaggcgggc
      241 gcggatgttc atcggtttgc ctgtggcgtg ttgctgtgtc cccgtctttt cataagtcca
      301 agttgggggc gagttgtaaa ttcgggctta aaagtgaggg tgagaccggg gaaatgcccc
      361 aaataggcca tccggcgcgc gtttcatctg tttcatgctt aaatgcggct taatgccgga
      421 aagctgcaat taaggcgatc ttaagctttc gccgagggat gctaactgaa gtaatcctgc
      481 aacggtcgga cccggatggt atcggccttt tgcgcggcga tggccttggt ggcagcgagg
      541 atgccgggca gcgtggtgta gtagggcacc ttttgcagca tggcagtctg gcggatggat
      601 tttgaatcgg actcggacgc tttggattcc gtggtgttca ggacgagttg gatttcgccg
      661 ttcttgatgg cgtccaccac gtggggcctg ccttccaaaa ccttgttgat cttggaagag
      721 ggaatgcctt tgctttcaaa gaactgctgg gtgccgccgg tggccttgat gacaaagccc
      781 aggtccacca gcttgcgtat ggcgggaagg attttggact tgtcgggatc gttgaccgag
      841 acaaagacgg tgccggactg gggaatgcgc atgccggcgc caagctggct cttggcgaag
      901 gccatgtcgt aggtcttgtc cagccccatg acctcgccgg tcgaacgcat ctcggggcca
      961 aggagtacat ccacgccggg gaagcggtta aaggggaaaa ccgcttcctt tacagcgaca
     1021 tgtgcgagct tcctgttgac cagcttgaag ctggcgaggg tttcgcccgc catgacccta
     1081 gcagcaatct tggccacggg aataccgatg accttggcca cgaagggcac ggtgcgcgag
     1141 gctctcgggt tcacttcgag gacgtagacc agatcatcct tgatggcgaa ctgcacgttc
     1201 atcaggccaa caacattcag tgccagggcc attttcctgg cctgggcctc catttcggca
     1261 atgacggggg ctttcaggga ataggggggg agcgagcagg cgctgtcgcc cgagtggatg
     1321 ccagcttctt cgatatgttc catcacacca gcgatgaaca cttccctgcc gtcgcaaatg
     1381 gcgtcgacgt caacctcgac ggcgttggag agatagctgt caatcagcac cgggctgtca
     1441 ccggagacga ctacggcttc ggtgatgtag cgctggaggt ggtcgtcgtc gcggacgatt
     1501 tccatggcgc ggccgcccag cacataggag gggcggatga ccacggggaa gccgattttc
     1561 cgggcgatct tcttggcctc cgcggcggaa cgggcgatgc cgttctcggg ctggcgcagc
     1621 ttcagtttct tgagaagcct ggagaaacgg tcacggtctt cggcaagatc aatggcatcg
     1681 ggcgtggtgc cgagaatggg aacgccgagc tcttccagtt ttcctgcgag cttcaacggc
     1741 gtctggccgc cgaactggac gatgacgcct ttcagggcgc cgttggattg ctcgacgtga
     1801 atgatttcga gaacgtcttc gatggtgagg gattcgaaat agagacggtc cgaggtgtcg
     1861 taatcggtgg aaacggtttc cggattgcag ttcaccatga tggattcata gccggcgtca
     1921 tggagcgcga agcaggcatg gcagcagcag taatcgaact cgatgccctg gccgatgcga
     1981 ttgggcccgc cgccgaggat gatgaccttt tgcctgtcag agggcttcgc ttcattgccg
     2041 ttgcggccga tgaaacccgt ttcataagtt gaatacatgt aggcggtggg cgaggcgaat
     2101 tcggccgcgc aggtatcgat gcgtttgaag acggggcgca cattcagctt gtggcgatgc
     2161 ttgcggacct tgtcttcgtc cacgccaacc aatgccgcga ggcgcctgtc ggagaatccc
     2221 atggccttga gtttctggaa attcaccgca tcgttgggca ggccgttagc gcggatcttc
     2281 gcttcggttt gcaccagttc ttcgatctgg cggaggaacc agggctcata cttgcaggag
     2341 gcaaaaatct gttcgaccgt ggcgccatgg cggaaggcct gggcgatgat gagcaggcga
     2401 tcgggggttg gcgttcccag ggcggcgcgg atggcgttct tgtcgtcgcc ttcaccgagt
     2461 cccgggattt caatttcatc gaagccgctg aggccggttt caaggccgcg aagggctttc
     2521 tgcacgcttt cctggaaggt gcggccgatg gccatgactt cgccgaccga cttcatggcg
     2581 gtcgaaagaa tggcttgcga gccggggaat ttttcaaagg caaagcgcgg aattttggtg
     2641 acgacgtaat ctatcgtcgg ctcgaaggcg gcgggggtgg cgccgccggt gatgtcattg
     2701 gccaattcat ccagcgtgta gccaactgca agcttggcgg cgatcttggc gatgggaaag
     2761 cctgttgctt ttgaggcaag ggcggaggag cgcgagacgc gcgggttcat ttcgatgaca
     2821 accaagcggc catcggcggg attcacgccg aactggacgt tggagccgcc ggtttcgacg
     2881 ccaatctcgc gcagcaccgc aatcgaggcg ttgcgcatga tctggtattc tttgtcagtc
     2941 agcgtcaggg ccggggctac ggtgatggaa tcgccggtgt gtacgcccat gggatcgacg
     3001 ttttcgatgg agcagacgat gatgc
//
LOCUS       DAORVN010000205         3016 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_29108_length_3016_cov_3.126984, whole genome shotgun sequence.
ACCESSION   DAORVN010000205 DAORVN010000000
VERSION     DAORVN010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3016
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29108_length_3016_cov_3.126984"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="PKV23_05140"
     CDS             complement(<1..334)
                     /locus_tag="PKV23_05140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX84854.1"
                     /translation="MDYFIRNAGTSRASGIIATLCKCTLIWLAVGLVLLPGTVLEAGA
                     KTYREIALASLKNLPADARIRADLEASIAGQANAYRHSKGIASLQASSRLRDAARAQA
                     VDMMLNGYV"
     gene            462..1829
                     /locus_tag="PKV23_05145"
     CDS             462..1829
                     /locus_tag="PKV23_05145"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing phosphodiesterase"
                     /protein_id="HQX84855.1"
                     /translation="MNELSALRKYLQQFSSYDQDTGFPNCETLLAEINTETTKASPLP
                     LGATLIEIRIRGLSRIGEAYSRKAIGSVIRQLADRLALAPLTKRTIARIDHKSFGVFL
                     PDVADPVLALQTAKQLVALCSRPVPWGDHMLNIDAIAGVAMTTPAVQDANTLLHHAGL
                     ALRASSENGSPGYAFFDPTEARAAKRRSDLVSIVAKAVEQDQFEMVYQPFFAFDSGKL
                     VGFEALMRLRHSEFGQISPSEFIPIAEDNGLISRLGAWCIEDVCRTAVEWPSHLIAAV
                     NFSPAQFYSGTLLALVNQALERTKFPAYRLDIEITEGTFMRDQELVLSQLNSLRDMGC
                     SIALDDFGTGYSSLGYLWQFPFSKLKIDRSFIHALDTTPKAPSILKSIIDLSRNLGIE
                     VTAEGIETTEQMRTLRNLNCDYLQGFLCGVPTAKSNLAAIILKNFSETLNQENAKLPK
                     IRVIR"
     gene            1860..2624
                     /locus_tag="PKV23_05150"
     CDS             1860..2624
                     /locus_tag="PKV23_05150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX84856.1"
                     /translation="MDKLPAFQAIKHALKSVIQFRNAGIRIGLPWVLVLAGINLIDRS
                     FSPAPGPAGSAAGMMLPGVMELVIAALGLVIFSSIAVNWHRYILLDEVTASEKVFRLD
                     RPVWSYVGRTLLIMLITIAPVLIFSLAFVNTLPNLGFLLAFPFFVAGIYIMRMSVALP
                     AIALDRTDFGISAALQVTQGNNLQFAGLLALNGLILFATFLALGIVLSIVGGASLTAA
                     KFLALVLSVPVNLFLSLFSVSLLSSLYGFFVEKRKF"
     gene            complement(2625..>3016)
                     /locus_tag="PKV23_05155"
     CDS             complement(2625..>3016)
                     /locus_tag="PKV23_05155"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HQX84857.1"
                     /translation="GRKRRCGWFDAVLVRQAIKTSGIHGIALTKLDVLDGLPEIKVGV
                     GYRLDGKRIEQFPAAEEAQKRVEPIYENFEGWKDTTAGARSWAQLPAQAIKYVKYLEE
                     LIECPVALLSTSPEREDTILMKDPFED"
BASE COUNT          599 a          995 c          749 g          673 t
ORIGIN      
        1 ccacatagcc gttgagcatc atgtccaccg cctgggcgcg cgccgcatcg cgcagccgtg
       61 agctcgcttg cagggacgca attccttttg aatgccggta ggcattggcc tgccccgcaa
      121 ttgaggcctc aagatctgcc cgaatgcggg catcggcagg caggttcttg agcgaagcca
      181 gggcaatttc gcggtaggtt ttggcgcccg cctcaaggac cgtccccggc agtaaaacca
      241 gccccactgc gagccaaatc agcgtgcact tacataaggt tgcaattatc cccgatgctc
      301 gggaggttcc cgcattgcgg ataaaatagt ccatatggcc ctcagtcaaa cgcaaaattt
      361 tacacattct ttcattctta ataaccatta tggcctgatc ggaagccaag gccaaattgt
      421 cttaaatgaa cctttccgcc gccccagaac acgtccctga cgtgaacgaa ttgagtgccc
      481 tgcgcaagta cttgcagcag ttctccagct atgaccagga taccggcttc ccgaattgtg
      541 agacccttct agccgagatc aacacggaaa ccaccaaggc cagtcctctc cctcttggcg
      601 ccaccctcat cgaaatcagg atcaggggcc tctccagaat aggcgaagcc tatagccgca
      661 aggccattgg ttcggtcatc cgccaattgg ccgaccgcct ggcgctcgcc cctctcacca
      721 agaggaccat cgcccggatc gatcacaaga gcttcggcgt tttcctcccc gacgttgccg
      781 accctgtgct ggcccttcaa accgccaagc agctggtggc attgtgttca aggcccgtgc
      841 cctggggtga tcacatgctg aacatcgatg ccatcgccgg cgttgccatg accacccctg
      901 ccgtccagga cgccaacacg ttgctgcatc atgccgggct cgccctgcgc gcctcgtcgg
      961 aaaacggcag ccccggctac gccttctttg atccaaccga agcccgcgcc gcaaagcgcc
     1021 gcagtgatct cgtttccatc gtcgccaagg cggttgagca agatcagttt gaaatggtct
     1081 accagccgtt ttttgccttc gactccggca agctggtggg cttcgaagcc ctgatgcgtc
     1141 tgaggcattc cgagttcggc caaatcagcc ctagcgagtt catccccatc gccgaggaca
     1201 acggcttgat ttcaaggctt ggcgcctggt gcatagaaga tgtctgccgc accgccgtcg
     1261 agtggccctc gcacctcatt gccgccgtca atttttcccc cgcccagttc tattcaggaa
     1321 ccctgcttgc cctagtcaac caggcgctgg agcgcaccaa gtttccggcc tatcgactcg
     1381 acatcgagat taccgaaggc accttcatgc gcgaccagga actggttctc tcgcaactca
     1441 actccctccg cgacatgggc tgctccattg cccttgatga ctttggcacg ggctattcaa
     1501 gcctcggcta cctctggcag tttcccttct cgaaactgaa gattgaccgc tccttcatcc
     1561 acgccctcga caccacgccc aaggcgccaa gcatcctcaa gtccatcatc gatctcagcc
     1621 gcaacctggg catcgaggtg acagccgaag gcatcgagac aaccgaacag atgcgcaccc
     1681 tgcgcaacct gaattgtgac tacctgcagg gctttctctg cggcgtgcca accgccaaat
     1741 cgaacctggc agccattatc ctcaagaact tctctgaaac tctgaatcag gaaaatgcga
     1801 agcttccaaa gataagagta attcgctaag accctgctgg aaaagagtac ccaggaccca
     1861 tggacaagtt gcccgcgttt caggccatca aacatgcctt gaagtcagtc atccagttcc
     1921 gcaacgccgg aatcaggatt ggcctgccct gggttctggt gctcgccgga atcaacctca
     1981 ttgaccgcag cttttccccg gctcccggtc cggcaggcag tgccgccggc atgatgctgc
     2041 caggcgtcat ggagcttgtt atcgccgcgt tgggactggt gattttttcc tccatcgccg
     2101 tgaactggca ccgctatatc ctgcttgatg aagtgactgc ctccgaaaag gtcttccggc
     2161 ttgatcgccc cgtctggagt tatgttggcc gcaccctgct gatcatgctg ataacaatcg
     2221 cgcccgtgct catattctcc ctcgctttcg tgaacaccct gccgaacctc ggcttcctgc
     2281 tcgctttccc cttcttcgtc gccggaattt acatcatgcg catgtccgtt gccttgcccg
     2341 ccattgccct ggaccgcacg gacttcggca tctcggcggc cctgcaggtg acccagggca
     2401 acaatctgca gtttgcaggg cttcttgcgc tcaatggact gatccttttc gccacatttc
     2461 tggcgctcgg catcgtcctt tccattgtcg gcggagccag cctgacagcg gcaaaattcc
     2521 tcgccctggt gctgagcgtg ccggtgaatc tttttctctc gctcttcagc gtcagccttc
     2581 tgagctcgct ctatggtttc tttgttgaaa agcggaagtt ctgatcaatc ctcgaacgga
     2641 tccttcatca ggattgtgtc ctcgcgctca ggcgacgttg aaagaagcgc cacgggacat
     2701 tcgatcagct cctcaaggta tttcacatac ttgatggcct gtgccggaag ctgggcccag
     2761 gaacgggctc ccgccgtggt atccttccag ccttcgaaat tctcatagat cggctcaacc
     2821 cgcttctgcg cttcttccgc cgccggaaat tgttcgatgc gcttgccgtc aagcctgtag
     2881 cccacaccca ccttgatttc cggcaatcca tcgagcacgt ccagcttcgt cagcgcgatg
     2941 ccgtgaatgc ccgatgtctt gatggcctgc cgcaccagca ccgcatcaaa ccagccgcag
     3001 cggcgcttgc gcccca
//
LOCUS       DAORVN010000206         3007 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_29253_length_3007_cov_3.597900, whole genome shotgun sequence.
ACCESSION   DAORVN010000206 DAORVN010000000
VERSION     DAORVN010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3007
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29253_length_3007_cov_3.597900"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..821)
                     /locus_tag="PKV23_05160"
     CDS             complement(<1..821)
                     /locus_tag="PKV23_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018236545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HQX84858.1"
                     /translation="MPDFILAIDQGTTSSRAIVFDAKLRPRASAQKEFKQYFPNSGWV
                     EHDPEDIWKSVLATCKAAIGKAKTAPKNIRCLGITNQRETTVVWERKTGKAIHKAIVW
                     QDRRTAETCAALKAQGHEKLFNAKTGLLLDPYFSGTKVSWILDQVKGARERAARGELC
                     FGTIDSFLIWRLTGGKSHVTDATNASRTLLYDIHSGGWDDELLSILRVPRAMLPEVKD
                     CAADFGMCEARHFGVAIPILGVAGDQQAASVGQACFVPGMMKSTYGTGCFALLNTG"
     gene            complement(826..2340)
                     /gene="glpD"
                     /locus_tag="PKV23_05165"
     CDS             complement(826..2340)
                     /gene="glpD"
                     /locus_tag="PKV23_05165"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HQX84859.1"
                     /translation="MIYDVFVIGGGVNGCGIARDAAGRGLKVFLAEQGDLASGTSSAS
                     TKLIHGGLRYLEHYEFRLVREALVEREVLLKAAPHIIWPLRFVLPHHKGLRPRHLIRL
                     GLFLYDHLGGRKILPPTRSIDLHSDVTGEPLKAEFAHAYEYSDCWVEDARLVALNARD
                     AANRGAEIHTRTKCTSARRENGAWDITILPEGGKEQTIRAKALVNAAGPWVSEVLGSI
                     VGRNNPDRIRMVKGSHIVVDRLYDHDRCYIFQNADGRICFAIPYEQDFTLIGTTDEDH
                     VGEPGNPQITESETDYLLASTSEYFKLPITREQVRWTYSGIRPLYDDGASKAQEATRD
                     YVLKLDAPSGQAPLLSVFGGKITTFRRLAESVLEKLGPFFPAARKPWTADAALPGGDF
                     PFDEVEARIAELQRKYSFFSPQNVRRIFRAYGTLAEKMFDNARFATDMGRSFGPLSER
                     EINYLIAEEFVREPDDILWRRSKLGLHLSADEQKALRDYMKPAVKEKSSKRAKG"
     gene            complement(2337..>3007)
                     /locus_tag="PKV23_05170"
     CDS             complement(2337..>3007)
                     /locus_tag="PKV23_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009208998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HQX84860.1"
                     /translation="DGRLLARTHGGAMLTSGVENVAYEARRQLAALEKQAIGQRAASL
                     IPNNCSLFINIGTTTEEVARALVHHEGLLVITNNIHVATILMPCTKIDVIIAGGAVRR
                     SDGGIVGEQAVDLIAQFKVDHAVIGVSAIDDDGAMLDFDYREVRVAQAILNNARNIIL
                     VSDAMKFTRSAPVRIGHLSQVDVFVTDRAPPDSIVELCEHSGVELEISDAGQSYDSTI
                     QAAE"
BASE COUNT          555 a          947 c          894 g          611 t
ORIGIN      
        1 cccgtattga gcagggcaaa gcagcccgtt ccgtaggtcg atttcatcat gcccggtaca
       61 aagcaggctt ggccgacgct ggctgcctgc tggtcaccgg ccacgccgag tatgggaatg
      121 gccacaccga aatgcctggc ttcgcacatg ccgaaatccg ccgcgcaatc cttgacctcc
      181 ggcagcatgg cgcggggcac gcgaaggatg ctgagcaatt catcatccca gcctccactg
      241 tggatgtcat agagcagggt gcgtgacgca ttagtagcat ccgtcacatg ggatttgccg
      301 cccgtgagcc gccagatcag aaacgagtcg atcgtgccga agcaaagttc gccgcgcgcc
      361 gcccgttccc gcgcgccttt cacctggtcg agaatccatg acacttttgt gccggagaaa
      421 tagggatcaa gcagcagccc ggtcttggcg ttgaaaagct tctcatggcc ctgggctttc
      481 aacgcggcgc aagtctccgc cgtccgccgg tcctgccaga cgatggcctt gtggatggcc
      541 ttgcctgtct tgcgctccca caccaccgtg gtttcgcgct ggttggtgat gcctaggcag
      601 cggatatttt tgggcgcggt cttggctttg ccaatcgccg ccttgcaggt ggccagcacc
      661 gatttccaga tgtcttccgg gtcatgctcg acccagcccg aattggggaa atactgcttg
      721 aattcctttt gtgccgaggc cctgggcctg agcttcgcgt caaagacaat ggcgcgtgac
      781 gaggttgtgc cctgatctat ggccagaatg aaatcaggca tcgccttacc ccttcgcgcg
      841 cttggaactc ttttctttta cagcaggctt catgtagtcg cgcaacgcct tctgctcgtc
      901 ggctgaaaga tgcaggccaa gctttgacct gcgccacagg atgtcatcgg gctcgcgcac
      961 gaattcctcg gcgatgagat agttgatctc gcgctcgctc aaagggccaa agctgcgccc
     1021 catgtccgtg gcaaagcgcg cgttatcgaa catcttttca gccagcgtgc cataggcgcg
     1081 gaagatccgc cggacgtttt gcggcgagaa gaacgaatat ttgcgctgca gctcggcgat
     1141 gcgcgcttcc acctcatcga agggaaagtc tccgccgggc agcgctgcgt ccgctgtcca
     1201 gggcttcctg gccgcgggaa agaatggccc cagcttttcc agaaccgatt ccgccagcct
     1261 gcggaaggtg gtgatcttgc cgccgaagac cgagagcagc ggcgcctgcc cgctaggcgc
     1321 atcgagtttc agcacatagt cgcgcgttgc ttcctgcgcc ttgctggccc cgtcgtcgta
     1381 aagcggcctg atgcccgaat aggtccagcg cacctgctcg cgggtgatcg gcagcttgaa
     1441 atattcgctc gtcgaggcca gcaggtaatc ggtctcgctc tcggtgatct gcgggttccc
     1501 cggttcaccc acatggtcct cgtccgtcgt gccgatcagg gtgaaatcct gttcataggg
     1561 aatggcaaag cagatgcgcc cgtcggcatt ctggaaaata tagcagcggt cgtggtcata
     1621 gagccggtca accacgatgt ggctgccctt gaccatgcgg atcctgtccg gattgttgcg
     1681 ccccacgatg ctgcccagaa cttcgctcac ccatggccct gccgcattga caagggcctt
     1741 ggcccggatg gtctgttcct tgccgccctc cggaagaatg gtgatatccc aggcgccgtt
     1801 ttcccgccgg gcgctggtgc attttgtccg cgtgtggatt tccgcacccc ggttggccgc
     1861 atccctggca ttgagcgcca ccagccgcgc atcctccacc cagcagtcgg aatactcata
     1921 ggcatgggca aattccgcct tcagcggctc gccggtcaca tcgctgtgca ggtcaatgct
     1981 gcgcgtcggc ggcaggatct tgcgcccgcc gagatggtca taaaggaaaa gcccgaggcg
     2041 gatgagatgg cgcggccgca ggcccttgtg atgcggcagc acgaagcgca gcggccagat
     2101 gatgtgcggc gccgccttga gcagcacctc ccgttcaacc agggcctcgc gcaccaggcg
     2161 gaattcatag tgctcgagat agcgcaagcc gccatggatc agcttggtgg aggccgagga
     2221 cgtgccggag gccaggtcgc cctgttcggc gaggaacacc ttcaggcccc gccctgccgc
     2281 atcacgcgca atgccgcaac cattgactcc gccgccgata acaaacacat cataaatcat
     2341 tctgcggcct gtattgtgga atcgtaactc tgtccggcgt cggaaatctc aagctcgacg
     2401 ccgctgtgct cgcacagttc aacgatggaa tcgggcggtg cccggtctgt cacaaatacg
     2461 tcaacttgcg aaagatgacc gatgcgcaca ggcgccgagc gggtaaactt catcgcgtcc
     2521 gacaccagga tgatgttgcg cgcattattc aggatggcct gcgccacccg cacctcgcgg
     2581 taatcgaaat ccagcatggc gccgtcatca tcgatggctg atacgccaat taccgcgtgg
     2641 tccaccttga actgggcgat cagatccact gcctgctcgc ccacgatgcc gccatcgctg
     2701 cggcgcaccg cgccacccgc aatgatcaca tcgatcttgg tgcagggcat gagaatggtg
     2761 gccacgtgaa tgttgttggt gatcaccaga agcccctcgt ggtgcaccag cgcccgcgcc
     2821 acctcttcgg ttgtcgtgcc gatgttgatg aacagcgagc agttgttggg aatgaggctt
     2881 gcggcgcgct ggccgatggc ctgcttttcc agggcggcga gctgccggcg cgcctcgtag
     2941 gcgacgtttt caacccccga ggtgagcatc gccccgccat gggtgcgcgc taacaatctt
     3001 ccgtcac
//
LOCUS       DAORVN010000207         3007 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_29254_length_3007_cov_3.506775, whole genome shotgun sequence.
ACCESSION   DAORVN010000207 DAORVN010000000
VERSION     DAORVN010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3007
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29254_length_3007_cov_3.506775"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            23..625
                     /locus_tag="PKV23_05175"
     CDS             23..625
                     /locus_tag="PKV23_05175"
                     /inference="COORDINATES: protein motif:HMM:NF016180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit gamma family protein"
                     /protein_id="HQX84861.1"
                     /translation="MSELYAESPLAHREAPQGLSIELREITDRGMIDVRGHATDKKFM
                     AAAKKVLGLDLPKAPRTSVSWGDVKALWLSIDQWLIICSRAKALELTAALKAELGNIH
                     SLVVDVSDMRAVLRLEGEGLREVLLKGSSLDLLGEDYAPGTVRRMRYAEIAALLHVVE
                     ETVFDVYVFRSYADYAWDFILATARAPAKVTLFGAQATPK"
     gene            841..1053
                     /locus_tag="PKV23_05180"
     CDS             841..1053
                     /locus_tag="PKV23_05180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX84862.1"
                     /translation="MKKFAISLLALAAVSTAALASNRDVTTPYGAQDTKQVGVVADSN
                     AFAVPAGSISIGNAGDDLGLGGSNRR"
     gene            1263..1463
                     /locus_tag="PKV23_05185"
     CDS             1263..1463
                     /locus_tag="PKV23_05185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX84863.1"
                     /translation="MKKIAISLIALAALSTASYASYRDDVVYVGPDAKQMSAESTDAN
                     AMVVIKKNTSGDDMELGGSNRR"
     gene            complement(1521..2378)
                     /locus_tag="PKV23_05190"
     CDS             complement(1521..2378)
                     /locus_tag="PKV23_05190"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HQX84864.1"
                     /translation="MDNPSRAFLKNLSLEITARQIEKLPILQERLPKGTPVFIALIDP
                     ADQAGQLAASSALRKAGLEPIPHVPARFVLDESDLKNRIAALSGEAGVTQMLVLGGGA
                     PTPMGKFDAAIQLLQTDVFQKNGVTRIGIAGHPEGNADITKVHGEAVLLKALGEKQAW
                     LKANKISGFIATQFLFEAAPVAYWASMLRDNGIDLPIHVGIPGPATIKTLVKYAAMCG
                     VGNSARFIRKQALNITKLLTVNTPDEFIEQLAVLHFDRPELGIQGPHLYPFGGFDKMF
                     EWLTPKLNG"
     gene            2608..>3007
                     /locus_tag="PKV23_05195"
     CDS             2608..>3007
                     /locus_tag="PKV23_05195"
                     /inference="COORDINATES: protein motif:HMM:NF014068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HQX84865.1"
                     /translation="MFGDKPKDVPQPFALILVPEFTMMPVTSAIEPLRLANRMSEKAL
                     YRWTMHSVDGQPVAASNNILTMVNGDLETVPPHATIIVCAGLNVQHHSDKRLISWLRK
                     TARKGNDIGAVCTGAHLLAEAGILDDYKCTI"
BASE COUNT          649 a          874 c          845 g          639 t
ORIGIN      
        1 gacaaggaag gggcgcggca gaatgtctga gctctatgct gaatctcccc tcgcccaccg
       61 cgaggccccg cagggcctca gcatcgagct ccgcgaaatc accgaccgcg gcatgattga
      121 tgtgcgcggg cacgccactg acaagaagtt catggccgcg gcaaaaaaag ttctgggcct
      181 tgatctgccg aaggcgccgc gcacgagcgt atcctggggt gatgtgaaag ccctgtggct
      241 ctccatcgac cagtggctca ttatctgttc gcgcgccaag gcgcttgaac tcaccgccgc
      301 gttgaaagcc gaactcggaa acattcattc ccttgttgtt gatgtctccg acatgcgcgc
      361 ggtcctgcgc cttgaaggcg aggggttgcg cgaagtcttg ctgaaaggct catcgcttga
      421 cctgctcggc gaagattatg cgccgggcac ggtgcggcgc atgcgctatg ccgaaatcgc
      481 ggcactgctg catgtggtgg aagagacggt atttgatgtt tatgttttcc gctcctacgc
      541 cgattacgcg tgggacttca tccttgcgac ggctcgcgcg cctgcgaaag tcaccttgtt
      601 tggggcgcaa gccactccca aatagggggc tgaaacagcc cctgcacgac ctttatgcag
      661 ccccctgttg tccccaagtt tttcatctaa cctattgata ttattatatc aaaaatatgt
      721 gatcagaaat attacatttt ttcttgaaac ggcatcccgg cttcttacat cgaggatatc
      781 gaaactgcga agagcgcagt tcacgagcta agatcaacaa gagaagtaaa ggatactatc
      841 atgaagaagt tcgcaatttc cctcctcgcc ctggccgctg tttctacggc tgcgcttgcc
      901 tcgaaccgtg atgtcaccac gccatatggc gcgcaggaca ccaagcaggt cggtgtcgtt
      961 gccgatagca acgcgttcgc tgttccggcc ggctccatct ccatcggcaa cgccggtgac
     1021 gatctgggcc tgggcggcag caaccgccgc taacgcctgc aataacaagc aacaacggcc
     1081 gggcatcacg cccggcctta tacttttcac aagaccgtga acccggactg cgccgcataa
     1141 cagcttcgtg accagcttgt ctttggcggt ttttctgaaa ctaagtgagc agcccagccg
     1201 tcttggtagt cagatcagtc attgaagacc ggtcgcgaaa cagaaacaca ggagactacc
     1261 ccatgaagaa gatcgcaatt tccctcatcg cccttgccgc cctctcgacc gcttcatatg
     1321 cttcataccg cgacgacgtt gtttacgttg gcccggatgc aaagcagatg tctgctgaat
     1381 cgaccgatgc aaatgccatg gtcgtcatca agaagaacac cagcggtgat gatatggaac
     1441 ttggcggaag caaccgccgc taatacttta gaattccaat tgctttggcc gggcgatagc
     1501 gtccggccac tcgcatttga tcagccgttg agtttcggcg tcagccattc gaacatcttg
     1561 tcgaagccgc cgaaaggata gagatggggg ccctgaatgc cgagctcggg gcggtcgaaa
     1621 tgcagcacgg cgagctgctc gatgaattca tcaggagtgt tcaccgtcag aagcttggtg
     1681 atgttcaatg cctgcttgcg gatgaagcgc gccgaatttc ccacgccgca catggcggca
     1741 tatttcacca gcgtcttgat cgtggccggt ccgggaatgc ccacatggat gggaaggtcg
     1801 atgccattgt cgcgcagcat gctggcccaa taggccaccg gtgctgcttc aaacaagaac
     1861 tgcgtggcga taaagccaga aattttgttg gccttgagcc atgcctgctt ttcgcccagc
     1921 gctttcagca acacggcttc gccatgaacc ttggtgatat cggcattgcc ttcgggatgg
     1981 ccggcgatgc cgatgcgcgt cacgccattt ttctggaaca cgtcggtctg cagcaattgg
     2041 atggctgcat caaactttcc catgggggtg ggtgctccgc cgcccagcac cagcatctgg
     2101 gtcacgccgg cctcgcctga cagggcggca atgcggttct tcaaatcact ttcatccagc
     2161 acgaagcggg ccggcacatg cggaatgggc tcaaggcccg ccttgcgcaa ggcgctggac
     2221 gccgccaatt gccccgcctg gtcggccgga tcgatcaggg cgatgaagac gggcgtgccc
     2281 ttgggcaggc gttcctgcaa gatcgggagc ttttcgatct gcctggccgt aatttccagc
     2341 gacaggttct tcaaaaacgc ccgggaagga ttgtccatca aatgggattt tgcagggttg
     2401 agaaagttgg gagcattcaa gacaagggcc tccaggcggg ttgaccctca aaatggcggc
     2461 aaccccccct attcgcccaa agatttacga caggcggcat acgaaaccga ccctttacct
     2521 tgccgcaagt cggtttttga ctgcgacatg gcgctggtgg acaaagtgtt ggaactgtcg
     2581 caaagatgcg gccttcggag aaaacccatg tttggcgaca aacccaagga cgtgccgcag
     2641 ccctttgccc tgattctggt gccggaattc accatgatgc cggtgacctc agccattgag
     2701 ccgttgcggc tggcaaaccg catgtcggaa aaggcgcttt accgctggac catgcattca
     2761 gtggatggcc agccggtggc cgcctccaac aacatcctca ccatggtcaa tggcgatctc
     2821 gagacggttc cgccacacgc aaccattatt gtctgcgccg gcctcaatgt gcagcaccat
     2881 tcggacaagc ggctgatctc atggctgcgc aagacggcgc gcaagggcaa tgacatcggc
     2941 gcggtctgca ccggggcgca cttgctggcg gaagccggca ttctcgatga ttacaagtgc
     3001 accatcc
//
LOCUS       DAORVN010000208         3006 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_29284_length_3006_cov_2.852931, whole genome shotgun sequence.
ACCESSION   DAORVN010000208 DAORVN010000000
VERSION     DAORVN010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3006
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29284_length_3006_cov_2.852931"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..758)
                     /locus_tag="PKV23_05200"
     CDS             complement(<1..758)
                     /locus_tag="PKV23_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005856715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HQX84866.1"
                     /translation="MSYELIALSMFGAMMVMLLTGQRVFAAIGFVASGAALLLYGTGA
                     IEMPYSQVFKLFNWFPMLTLPLFIYMGYVLSEAGIAEDLYRMLHVWFGRLRGGLAVGT
                     ILLMVIISAMNGLSVAGMAIGATIALPEMLRRGYDKVMITGVVQGGSSLGILIPPSVV
                     LVLYGMIARQPVGQLWLAAVVDRPFRAAPIPIPAPTPPAQQGEYFQNLAVLAWEDGAL
                     VSDAHRLAAGLDLSALAEPGFHTVVFTDASGRHD"
     gene            complement(755..1267)
                     /locus_tag="PKV23_05205"
     CDS             complement(755..1267)
                     /locus_tag="PKV23_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HQX84867.1"
                     /translation="MGKLARNYVRIVDYMSDKVGIVAMALIAAMIGILLLDAVTRNLI
                     RMPLHWCIELAQFTLTAYYFLGGAITLKNNDHVRMDLFYDRLSVKGKAWMDLVTSGCL
                     FFYLAVMLMGSISSLQYAIATGERRFSMWNPSMIPIKGLMTACLALMLLQTISLAIKH
                     TATIRGTSLS"
     gene            complement(1409..>3006)
                     /locus_tag="PKV23_05210"
     CDS             complement(1409..>3006)
                     /locus_tag="PKV23_05210"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HQX84868.1"
                     /translation="HDAINAAAIEHYGYSREQFLKMASLDIRPPSEHQRALDDARNDF
                     SVDNGDKDWLHIKADGTEILVSSYAKSINYNGTDAAIVAVIDVTERRKQDARIQYLAE
                     HDALTGLPNRRLFLDLLSSSLSRKTQIAYCTSIILIDIDDFKGINDTLGHQVGDDLII
                     AVGNRLEERVGDRGIVARLGGDEFAILLPMLTELDAAGAAAKELVNAFSKPLKVGDYD
                     LLTGVSAGVSFSIGDSVDSSTLLRNADLALYRAKTDGRGIYRVYEPQMSLQLMLRREM
                     ERDLAMALARNEFAVHYQPLMELESGLEVGFEALLRWNHPDKGMISPASFIPIAESSG
                     LIVPIGNWVLEQSCKLAATWRKNLSVAVNISPAQFKSGQLVDVVCAALAKYGLAPHRL
                     ELEITESLLLEKSYETLEVLRSLKELGVVIALDDFGTGYSGLGYLNSFPIDKIKIDRS
                     FIKDLGSKPRSKELVRAAVNIGHSLGLMTLAEGIETQEQLGILRALGCQQGQGFLFSP
                     AIPASQIGDALLLHGSAGRKAIA"
BASE COUNT          632 a          874 c          939 g          561 t
ORIGIN      
        1 cagtcgtgcc gaccgctggc gtcggtgaac accaccgtgt gaaaacccgg ctcggccagg
       61 gccgaaagat cgagcccggc ggccagccgg tgggcgtcgc tcaccagcgc gccgtcttcc
      121 caggccagca ccgcgaggtt ctggaaatat tcgccctgct gggccggcgg cgtgggggct
      181 ggaatgggaa tgggtgccgc acgaaaaggc cggtccacca cggccgccag ccagagctgg
      241 cccacgggct ggcgcgcaat catgccgtag agaacgagca caacgctggg cggaatcagg
      301 atgccgagcg atgagccgcc ctgcaccacg ccggtgatca tcaccttgtc atagccgcgc
      361 cggagcatct ccggcagggc gatggtggcg ccaatcgcca tgccggcgac ggaaaggccg
      421 ttcatggccg aaatgatcac catgaggaga atcgtgccga cagccagccc gccgcgcagg
      481 cggccaaacc agacatgcag catgcggtac aggtcttcgg caatccccgc ttccgagagc
      541 acgtagccca tgtagatgaa gagcggcagc gtgagcatcg gaaaccagtt gaacagcttg
      601 aacacctggc tgtagggcat ctcgatggcg ccagtgccgt agagcagcag ggcagcgccg
      661 gaagcgacga agccgatagc ggcaaaaacc cgctggccgg tgagcagcat caccatcatg
      721 gccccgaaca tggacagggc gatcagttca tagctcatga gagggatgtt ccccggatgg
      781 tggcggtatg cttgatggca agcgaaatgg tctgcagcag catgagagcc aggcatgccg
      841 tcatcaggcc cttgatcgga atcatcgatg gattccacat ggagaagcgc ctttcgccgg
      901 tggcgatggc atattgcagg ctcgagatgc tccccatcag catgacggcg agatagaaaa
      961 acaggcatcc actggtgacg agatccatcc aggccttgcc cttcaccgac agccggtcat
     1021 agaacaggtc catgcggacg tggtcattgt tcttcaatgt tatcgcgccg cccaagaagt
     1081 aataggccgt tagggtgaat tgcgcgagct cgatgcacca gtgcaaaggc atcctgatca
     1141 aattccgggt caccgcatca agcagcagaa tcccgatcat ggccgcgatc agtgccatgg
     1201 cgacaattcc caccttgtcg gacatgtaat cgactatgcg gacatagttc cttgcgagtt
     1261 ttcccattcg ctccccgtgg ccggcaccga cctgacgcag tcagatgggc gcacaaccct
     1321 tagctgccac caacctggca agaagaactg atacggaagc gcaagtcaac cggtattggc
     1381 gtagcagttg tcatcggtgg agtttgcgtc aggcaatggc cttccggccc gcactgccat
     1441 ggagcagcag ggcatcgccg atctggcttg cgggaattgc cgggctgaac aggaaaccct
     1501 ggccctgctg gcaacccagg gcgcgcagga tgccaagctg ttcctgggtc tcgatgcctt
     1561 cggcaagtgt catgaggccg aggctgtggc cgatattgac ggcggcccgg accagttcct
     1621 ttgaccgcgg cttgctgccg agatccttga tgaaggagcg gtcaatcttg atcttgtcga
     1681 ttggaaaact gttaagatag cccagccccg aatagccggt gccgaaatca tcgagggcaa
     1741 tcaccacgcc cagctccttc aggctccgca gcacttcgag cgtctcgtag gacttttcca
     1801 ggaggagcga ctcggtgatc tcgagctcaa gccggtgggg ggccaggcca tatttcgcga
     1861 gcgcggcaca gacgacatcg accagctggc ccgacttgaa ctgggcgggc gaaatattga
     1921 ccgcgacgga gaggttcttg cgccaggtgg cggccagctt gcaggactgt tcgagcaccc
     1981 agtttccaat gggcacgatc agtccgcttg attcggcaat cggaataaag gaggccggcg
     2041 aaatcatgcc tttgtccggg tggttccagc gcaggagagc ttcaaaaccg acttcgagcc
     2101 cgctttccag ctccatcaag ggctgataat ggaccgcgaa ctcattgcgg gccagggcca
     2161 ttgcgaggtc gcgttccatt tcacggcgca gcatgagctg cagcgacatc tgcggttcat
     2221 aaacccggta gatgccgcgg ccgtcggtct ttgcccggta gagagccagg tcggcatttc
     2281 tgagcagggt cgaagaatca accgaatcac cgatgctgaa actcacgccg gcgctgacgc
     2341 ctgtgagaag atcgtagtcg cccaccttga gcggcttgct gaaggcattc accaattcct
     2401 ttgccgcggc tccggcggcg tcaagctcgg tgagcatcgg aagcagaatg gcgaactcgt
     2461 cgccgccaag cctggcgacg atgccacggt cgccaacgcg ctcctccagc cggttgccca
     2521 ccgcaatgat gaggtcgtcg cccacctgat ggccaagcgt gtcgttgatg cccttgaaat
     2581 cgtcgatgtc gatgagaatg atggaggtgc aataggcgat ctgggttttc ctcgacaggg
     2641 agcttgaaag aaggtcaagg aaaagccttc ggttgggaag tccggtcagg gcgtcatgct
     2701 ccgcaagata ctgaatgcgc gcgtcctgct tgcggcgctc ggtgacgtca atgacggcaa
     2761 cgatggcggc gtccgtgccg ttgtaattga tggatttggc gtaactgctg accaggatct
     2821 cggttccgtc ggccttgata tgcagccaat ccttgtcacc gttgtccacc gagaagtcgt
     2881 tgcgcgcgtc gtcgagcgct ctctggtgtt cgcttggcgg acgtatgtcc agcgatgcca
     2941 ttttcaggaa ctgttcgcgc gaatagccgt aatgctcgat agcggcggcg ttgatggcgt
     3001 catgtg
//
LOCUS       DAORVN010000209         2996 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_29455_length_2996_cov_3.656579, whole genome shotgun sequence.
ACCESSION   DAORVN010000209 DAORVN010000000
VERSION     DAORVN010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2996
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29455_length_2996_cov_3.656579"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..625
                     /locus_tag="PKV23_05215"
     CDS             <1..625
                     /locus_tag="PKV23_05215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX84869.1"
                     /translation="SVQVGADAQKFNATKIDTLHKPVSPFFDADVCNFTPERFFWMSV
                     TAASGATVALQAFRYDYTDTSLADWGPNLTIGLAMRRQELMVPTNAAPPRNSIAERIR
                     GKLVYHGEFWIDQHVRNRKLAEKFSRLGLILSLIKWNPDAVWSLSSSRMATHGHLNRM
                     GYNYIEKGFLRWQWATDGIDLTEWLNIAERHAIEQMINEMITSPVPA"
     gene            complement(634..1422)
                     /locus_tag="PKV23_05220"
     CDS             complement(634..1422)
                     /locus_tag="PKV23_05220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX84870.1"
                     /translation="MEPRQRVEVMQGQRFWIAGGKKLPDSSIEHVLDDVEAERFNHDG
                     AVRVTTGPLGTEIKWSVFSPCFGSLYYAIEWLPLASLPIVLRYYLSGWFEETLFTAEQ
                     AINRIEQIIAKSELHLTRRTFVEEFDPKGKTLSPMLQKIWQDHTIWPENSVDCVYEEQ
                     SRKFRVDRIGAQSTIAKLYGMSPVSYACKSGNSYDEVVSEAYTHVLRSGKPRYDHVLA
                     ALRMPDNIVRWVPYQRIVVPRPDSEKSMSVSVVAEIAKIDISLI"
     gene            complement(1593..>2996)
                     /locus_tag="PKV23_05225"
     CDS             complement(1593..>2996)
                     /locus_tag="PKV23_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015318278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HQX84871.1"
                     /translation="EKCAELLTAKKLPLLADIRDESAEDIRIVLEPKSRTVDPTLMME
                     QMFRSTELEARIPLNMNVLSGGKVPMVMSLGDVLREWLEHRKTVLVRRTEFRLAEIAR
                     RLEILGGYLIAYLNLDEVIRIIREEDEPKPALMKRFKLTDTQAEAILNMRLRALRKLE
                     EMEIRTENAGLVKEQKGLKALLKSDDEQWLQISEEIKAVKEKFSKKTPLGRRRTDFAE
                     APEIDLELEQSMIEKEPVTIVCSEKSWIRAMKGHIEDTSTLSYKDGDKGKFVLNAQST
                     DKIMLFSSSGKFFTLEAGKLPGGRGHGEPVRLMADLDSTDSIVALFVHAPGTKRLLAS
                     TDGDGFIVLEDECVATTRKGKQVLNVKAPSEAQVCVPVDETDNHVAAVGDNRKLLIFK
                     LSELPEMTRGKGVRMQKFKDGGLSDARTFNLKEGLSWIDSSGRNFTVTELKDWLGERA
                     QAGRLPPKGFPKSNRFG"
BASE COUNT          631 a          868 c          822 g          675 t
ORIGIN      
        1 ttccgttcag gttggagcgg acgcgcagaa gttcaatgcc accaagatcg acacgctgca
       61 caagcccgta tcgccattct ttgatgcaga cgtctgcaat ttcacgccgg agcgtttttt
      121 ctggatgagc gtgactgcgg caagcggggc aaccgtggct ttgcaggcct tcaggtatga
      181 ctataccgac acgagcctgg cggattgggg gcccaacctc acaatcggcc tggccatgcg
      241 ccggcaggag ttgatggtcc cgacaaacgc cgcgccgccg cgcaacagca tcgccgaacg
      301 cattcgcggg aaactggtct accacggcga gttctggatc gaccagcatg tgcgcaaccg
      361 aaagctggcg gaaaagtttt cacgcctcgg cctcattctc agcctcatca agtggaaccc
      421 ggatgcggtg tggtccctga gcagcagccg catggcgacg catggccatc tcaaccgcat
      481 gggctacaat tacatcgaga aaggcttcct gcgctggcag tgggcaacgg acggaattga
      541 tcttactgaa tggctgaaca tcgccgagcg ccatgccatt gaacagatga tcaatgaaat
      601 gatcacaagc ccggtgccgg cataggccgc catttatatc aggctgatgt cgatcttggc
      661 gatttccgcc acaacgctca cgctcattga tttttcgctg tccggccttg gcacgacaat
      721 cctctgataa ggcacccagc ggacgatatt gtccggcatg cgcaaggccg ccagcacatg
      781 atcataacgc ggcttccccg aacgcagcac gtgggtatag gcttcgctca cgacctcgtc
      841 ataggaattg ccgctcttgc aggcatagga caccggtgac atgccataga gcttggcgat
      901 cgttgattgc gcgccgatcc ggtcaacccg aaacttgcgc gattgttctt catagacgca
      961 atccacggaa ttctccggcc agatcgtgtg atcctgccat attttctgca gcatgggtga
     1021 aagcgttttg cccttcggat cgaattcttc gacgaaagtc cgcctggtca ggtggagttc
     1081 gctcttggcg atgatttgtt caatccggtt gatggcctgc tcggcagtaa acagggtctc
     1141 ttcaaaccat ccggaaaggt aatagcggag aacgatgggc agactggcca ggggcagcca
     1201 ttcaatcgcg taatacagcg acccaaagca gggtgaaaaa accgaccatt tgatttcagt
     1261 accaagcggg ccagtcgtca ctcttacggc gccgtcatga ttgaagcgct ccgcttcaac
     1321 gtcatcgaga acgtgctcga tggaagaatc ggggagcttt tttcccccag ctatccaaaa
     1381 tctctggcct tgcatgacct caacccgctg tcgcggctcc aataatcctt acagtccttc
     1441 cgtgaatgcc gtccgcaaac tgtaaccgtt ttctgcccac atcatgggtt cggcccgaat
     1501 aattttgcgt taagattctt aacgctattt agtatcagtt gaaacaataa ggcgatttta
     1561 aaggaaaaac cgcgccctgc gggccactcc tgttaaccaa agcggttgct tttggggaat
     1621 cccttgggtg gcagcctgcc cgcctgggcc cgttcgccca gccagtcctt gagttcagtc
     1681 acggtaaagt tgcgtcctga tgaatcaatc caggacaggc cttccttcag gttgaaggtc
     1741 cgcgcatctg acaacccgcc atccttgaat ttctgcatgc ggacaccctt gccgcgggtc
     1801 atctcgggga gctcggagag cttgaagata aggagcttcc gattgtcgcc cacggctgca
     1861 acatgattat ccgtctcatc caccggcaca cagacctggg cctcggaggg cgccttgacg
     1921 ttcagcacct gtttgccttt gcgggtggtg gccacgcatt catcctcaag gacgatgaaa
     1981 ccgtcgccat cggtagaggc gagcagccgc ttggtcccgg gcgcatggac gaacagcgcc
     2041 acgattgaat cggtggaatc aagatcggcc atcaggcgga caggctcgcc atggccacgg
     2101 ccgccgggaa gtttacccgc ttccagggtg aagaacttgc ccgatgacga gaacagcata
     2161 attttgtcgg tagactgggc attcaggacg aattttccct tgtcgccatc cttgtaggaa
     2221 agcgttgagg tatcttcgat gtggcccttc atggccctga tccagctctt ctcgctgcag
     2281 acaatggtaa ccggttcctt ctcgatcatg gactgttcga gttccagatc aatttcgggc
     2341 gcctcggcaa aatcggtgcg ccttctgccc agcggcgtct tcttgctgaa cttctccttg
     2401 acggccttga tctcttcgga aatctgcagc cactgctcat catctgactt cagcagggct
     2461 ttcaggcctt tctgctcctt gaccaggccg gcattctcgg tgcggatttc catttcctca
     2521 agcttgcgca aggcgcgcag ccgcatgttg agaattgctt cggcctgcgt gtcagtcagc
     2581 ttgaaacgct tcatcaatgc aggtttgggc tcatcctcct cgcggataat ccggatgact
     2641 tcgtcgaggt tgagataggc gatgagataa ccgccgagaa tttcaaggcg ccgggcaatc
     2701 tcagcgaggc ggaattccgt acggcggacc aggacggtct tgcgatgctc gagccactcg
     2761 cgcagcacat cgcccaagga catgaccatc ggcaccttgc cgccggagag cacattcatg
     2821 ttcaagggaa tgcgcgcctc aagctcggtg ctgcggaaca tctgctccat catcagggtg
     2881 ggatcaacgg tgcggctctt cggctccagt acgatgcgga tatcctcggc ggattcgtcg
     2941 cggatgtcgg ccagcagcgg cagcttcttg gccgtcagga gttcggcaca tttttc
//
LOCUS       DAORVN010000210         2982 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_29719_length_2982_cov_2.302016, whole genome shotgun sequence.
ACCESSION   DAORVN010000210 DAORVN010000000
VERSION     DAORVN010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2982)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2982)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2982
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29719_length_2982_cov_2.302016"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..646)
                     /locus_tag="PKV23_05230"
     CDS             complement(<1..646)
                     /locus_tag="PKV23_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008546363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HQX84872.1"
                     /translation="MSMVDPQLQADAKGVAGAESSEFASLLQREFKPKSEEARDAVVK
                     AVEALAKQALCQTQLVGNDVVKSIEAMIAQLDKKLSEQVNLIMHNPSFQKLEGAWRGL
                     HYLVNNTETDEMLKIRVMNITKSELHKNLKRFKGTAWDQSPVFKRIYEEEYGQFGGEP
                     FGCLVGDYYFDQTPPDVELLGEMAKISASAHAPFIAAASPALMQMDSWQELANPR"
     gene            complement(695..1195)
                     /gene="tssB"
                     /locus_tag="PKV23_05235"
     CDS             complement(695..1195)
                     /gene="tssB"
                     /locus_tag="PKV23_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018318177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HQX84873.1"
                     /translation="MADSSQKFIARNRAPRVQIEYDVELYGAEKKVQLPFVMGVLSDL
                     SGNPSEPLAPIAERKFLEIDVDNFDSRMKSMKPRAAFQVPNTLTGEGNLNVELTFESM
                     DDFTPAAIARKVDALNKLLEARQQLSNLITYMDGKTGAEELVGRLLNDPALLQTLASA
                     PKPSEG"
     gene            complement(1247..2305)
                     /gene="tssA"
                     /locus_tag="PKV23_05240"
     CDS             complement(1247..2305)
                     /gene="tssA"
                     /locus_tag="PKV23_05240"
                     /inference="COORDINATES: protein motif:HMM:TIGR03363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HQX84874.1"
                     /translation="MNVNLDALLEPFPGELPCGLNLEYDDDFRELEKAASGTPELEFG
                     AGNGDGVTRIEGVGPDWAKVRKLSEGLFSRTKDLRVAVFLTRALTHSDGLTGLEMGLR
                     LTLSLLEGFWDHVHPMLDRDDNDDPTERINALAGLAAESMVGSVRDAWVVRSRQIGML
                     AVRDIEVAAGRLQARSGTEALSEAQVSGLITAALGEDPELAVRVRNLDGLLKSIDALL
                     SERVGAAGGVDFKPLQSVLYPVRQVVERCTRGSGGDFDVPSPDATLADDGAVGAVKTS
                     AAPQGEINSRSDVLRTLDRLCDFLARTEPGSPVPMVLRRAQKMMNMNFLELMNDLAPD
                     GLNQAEKVVGARFDNPES"
     gene            2676..>2982
                     /locus_tag="PKV23_05245"
     CDS             2676..>2982
                     /locus_tag="PKV23_05245"
                     /inference="COORDINATES: protein motif:HMM:NF018391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1178 family protein"
                     /protein_id="HQX84875.1"
                     /translation="MIVLDLSCDREHRFEAWFASSEAFEAQRAAGLVGCPHCGSSDVR
                     RLPSAPYVQTSSHSAPAAPAAPNPQAIAARLIETLRSAAARSEDVGERFAEEARRIHY
                     "
BASE COUNT          612 a          958 c          786 g          626 t
ORIGIN      
        1 cacggggatt ggccagttcc tgccacgaat ccatctgcat caatgccggg ctggccgctg
       61 caatgaatgg cgcgtgtgcc gaagcggaga tcttggccat ttccccaagc agctccacat
      121 ccggcggcgt ttgatcaaaa taatagtcgc caaccaggca gccgaagggc tcaccaccaa
      181 attgaccgta ctcttcctcg taaatgcgct tgaacaccgg gctttgatcc caggccgtgc
      241 ccttgaagcg cttcaggttc ttgtgaagct ccgacttggt gatgttcatt acccggatct
      301 tcagcatctc gtcggtttcg gtgttattga cgagataatg aagcccgcgc catgccccct
      361 ccagcttctg gaagctgggg ttatgcatga tcagattgac ttgttccgac agcttcttgt
      421 cgagctgggc gatcatcgcc tcgatcgatt tgacgacatc gttcccgacc aactgggtct
      481 ggcaaagcgc ctgtttcgca agcgcttcga cggctttgac gacggcatca cgtgcctctt
      541 cggacttcgg cttgaactcc ctctgcagta gcgatgcgaa ctcgctgctt tctgccccgg
      601 caacgccctt ggcgtcggcc tgaagttgcg gatcgaccat gctcatattc tccggttgtt
      661 attggtagtg acgcgcagcg ggcttctcgc ccactcagcc ttctgagggc ttcggcgccg
      721 aggcaagggt ctgcaacaag gcaggatcgt tgagcaagcg ccccaccaac tcttcggcgc
      781 cggtcttgcc gtccatgtag gtgatcaggt tggacaattg ctggcgggct tcgagaagct
      841 tgttgagcgc atccaccttg cgtgcaatgg cggccggcgt aaagtcgtcc atgctttcaa
      901 aagtcagctc gacattgaga ttgccctcgc cggtgagggt attgggaacc tgaaatgctg
      961 cccgcggctt catcgacttc atccggctgt cgaaattgtc cacgtcgatt tccaggaact
     1021 tgcgctcggc aatcggagcc aaaggctcag aagggttgcc cgacaagtct gaaagcaccc
     1081 ccatgacgaa gggcaactgg acctttttct cggcaccata cagttccacg tcatactcaa
     1141 tctgaacccg gggcgcccgg ttacgggcaa tgaatttctg actgctgtct gccattttgt
     1201 gcctccatat ggggatagcg atattgcact gcgcaatttg ccggcatcac gactccgggt
     1261 tatcgaaacg cgctccaacg accttttctg cctgattcaa gccgtcaggc gcaagatcat
     1321 tcatgagctc aaggaaattc atgttcatca ttttctgggc tcgccgcaag accattggaa
     1381 ccgggctacc cggctcagtc cgggccagaa aatcacacag tcgatcgagc gtccgaagga
     1441 catcggaccg ggagttgatt tccccctgag gggcagcaga agtcttgacc gcacctaccg
     1501 cgccatcgtc tgccagcgtg gcgtcggggc tgggaacatc gaagtcgcct cccgaaccac
     1561 gggtgcatcg ctcgacgacc tgccggaccg gatacagcac tgactgaagc ggcttgaagt
     1621 cgacgccccc agccgcgcca actcgctcgg acagcagcgc atcgatgctt ttgagcaaac
     1681 cgtccagatt ccgcaccctg actgcaagct ctgggtcctc accaagagcg gccgtgatca
     1741 gtccactgac ctgcgcctca ctcaaggcct ccgtgccact ccttgcttgc agccgccccg
     1801 cagccacctc gatgtcacgg acggcaagca tcccaatctg acgggagcgc acgacccatg
     1861 catcgcgaac agaacccacc atgctttctg ccgccagacc cgccaaggcg ttgatccgct
     1921 ccgtggggtc atcattgtcg tcccgatcca gcatggggtg gacatgatcc cagaagcctt
     1981 cgaggagaga gagcgtgagc cgaagcccca tctccaggcc tgtcagcccg tctgaatgtg
     2041 tcagtgcccg ggtcaggaag acagcgacac ggagatcctt ggttcgggag aagagccctt
     2101 cggacagttt gcgcaccttg gcccagtccg gccccacgcc ttcgattcgg gtaacaccgt
     2161 ctccgtttcc cgcaccgaac tccagctcgg gagtaccact ggcagccttt tcaagctcac
     2221 gaaaatcgtc gtcgtactcc agattcagac cacaaggcag ctcgcccgga aagggctcaa
     2281 gcaatgcgtc cagattcacg ttcaccccca ccgcctcatc acccagaatg ggcaaagcat
     2341 catcatcaag accttccctc tggatgctag caagtggaaa gattatcttg gatcaaaaga
     2401 aaacggacta cacttttgcc gctgcccgta accctgcttg caggacaaga ttgataacat
     2461 attcgcatca tatttcatag cattcaggct tagcgccggc ctcgatccgc tctagctgtc
     2521 tcggggtgat gatttaagcc agtacctcaa tcaggcaata atccaccagt tcgctgtcac
     2581 acaatgatcg gaacgagcgg tattggctct cgccttcgta catactttca actcgccggg
     2641 aacctggcct gacagaacac tcactacctt ccaccatgat cgtcctcgac ctgtcctgtg
     2701 accgggagca ccgcttcgaa gcctggttcg catcgagcga ggccttcgaa gcccagcgtg
     2761 ccgccggcct ggtgggctgc ccccattgcg gcagcagcga cgtacgccgc ctgccctcgg
     2821 ccccctacgt tcaaacctcg tcccattccg cccctgcggc accagcagcc cccaaccctc
     2881 aggccattgc cgcgcgtttg atagagaccc tacgcagtgc tgcagcccgc tccgaagacg
     2941 tgggggagcg cttcgccgaa gaggcccgcc gcattcacta cg
//
LOCUS       DAORVN010000211         2978 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_29792_length_2978_cov_2.629148, whole genome shotgun sequence.
ACCESSION   DAORVN010000211 DAORVN010000000
VERSION     DAORVN010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2978
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29792_length_2978_cov_2.629148"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..338)
                     /locus_tag="PKV23_05250"
     CDS             complement(<1..338)
                     /locus_tag="PKV23_05250"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HQX84876.1"
                     /translation="MTPLEKIIGEIIAAEGPMPIDRYMGLCLGHPLHGYYMTRDPFGE
                     QGNFMTAPEISQIFGELIGVWCAAAWDAMGKPAQFNLVELGPGRGTLMADILKAAKVM
                     PGFREAARIHL"
     gene            complement(335..1156)
                     /gene="lgt"
                     /locus_tag="PKV23_05255"
     CDS             complement(335..1156)
                     /gene="lgt"
                     /locus_tag="PKV23_05255"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008124267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HQX84877.1"
                     /translation="MFTFAVLPFPNIDPVLFSIGPFAIRWYALAYIVGIVFGIWYMKR
                     LVSNPRLWAGTTPSATPKQIDDFFLLAMLGVVLGGRIGYLMFYGWTQILSDPLYILRV
                     WEGGMSFHGGFFGVIIASYIYARRQGIGLDRLLDLGAAATPVGLGLGRLANFINGELY
                     GRETYLPWAFVFPNDLTGLPRHPSQLYELFLEGVLLFAVINYAVYRYKILTRPGRAAG
                     MYALIYGLSRIVVEFVREPDAQLGYFGGFVTMGMILSVPLLAIGVWLLLRSRRPA"
     gene            complement(1213..2400)
                     /locus_tag="PKV23_05260"
     CDS             complement(1213..2400)
                     /locus_tag="PKV23_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019196576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HQX84878.1"
                     /translation="MNNLATDVAIIGGGLAGATAAAMLGRAGHAAILIEPHAEQRPEF
                     RCEKLDDSQLELLSRTGLADDVLPHTCAGGKVWVVRYGRVVDRNKNSPAGIHYHDLVN
                     TVRQQIPASVPHIAAKVSAISGNAGRQRVSLANGDEIEARLVILANGLNPGLRETLEI
                     KREVLSPCHSISIGFDMKPEGDTPFAFPALTVFPERPDQSMAYLTVFPVPGAMRANLF
                     IYWNMEDPRLRKFRHQPREALFDLIPTLAQFTGPFTIDGQVSIRPVDLVRVKAPERPG
                     IVLVGDAYSTSCPAAGTGVNKVLTDVQRLCTTYVPAWLGKETIEAADVADFYADPVKV
                     QCEVESLQKALWLRRVSTGTSLRLRGWRGARFVVQSALGQLRGLGHPDAAAKGSTDLL
                     HGA"
     gene            complement(2393..>2978)
                     /locus_tag="PKV23_05265"
     CDS             complement(2393..>2978)
                     /locus_tag="PKV23_05265"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQX84879.1"
                     /translation="VGFQRARTSAEIDQVLAAFFAQKAARMREQGLSNAFDAAGVKEF
                     TRAAAHGIDPDTGEPVIELYALWVGGKIIATFGGIVADGRFSGMFNSIAGAEFRNYSP
                     GELLLANVIRMCCERGLQKFDLGIGDAAYKQVYCRDAETLYDSVIPITAAGQVAAPLW
                     RAGLAVKGKVKKSDTLLRAARFAAQMMSGNTPGE"
BASE COUNT          640 a          940 c          872 g          526 t
ORIGIN      
        1 agatgaatcc gcgccgcttc ccgaaagccc ggcatcactt tcgcagcctt gagaatgtca
       61 gccatcaggg ttccccggcc cggacccagt tccacaagat tgaactgtgc gggcttgccc
      121 atggcatccc acgccgccgc gcaccacacg ccgatcaatt cgccgaagat ctgcgaaatc
      181 tccggcgccg tcatgaagtt cccctgctcg ccaaacggat cgcgcgtcat gtaatagcca
      241 tgcagcggat ggccgaggca cagtcccatg tagcggtcta tcggcatcgg accctcggca
      301 gcaatgatct cgccgatgat tttttccagc ggcgtcatgc gggccgccgc gaacgcaaca
      361 gcaaccacac gccgatggca agaagcggca ccgacaggat catgcccatg gtgacaaagc
      421 cgccgaaata gcccaactgc gcatcgggct cgcgcacgaa ttcaaccacg atccgcgaga
      481 gaccatagat cagcgcatac atccccgccg cccggccggg cctggtcagg atcttgtagc
      541 ggtaaaccgc atagttgatc accgcgaaca gcagcactcc ttccaggaag agctcataaa
      601 gctggctggg atggcgcggc agccccgtga ggtcattggg aaacacaaag gcccagggca
      661 gataggtttc gcggccataa agctcgccat tgatgaaatt ggccagccgc ccgaggccga
      721 ggccaaccgg cgttgccgcc gcacccaggt ccagcagccg gtcaaggcca attccctggc
      781 gccgcgcata gatgtaggag gcgatgatca cgccaaagaa accgccgtgg aacgacatgc
      841 cgccttccca tacgcgaaga atgtaaagcg gatccgatag tatttgtgtc cagccgtaga
      901 acatcaggta gccaatgcgc ccgccaagca ccaccccaag catggccagg aggaagaaat
      961 catcaatctg ctttggcgtg gccgatggcg tggttccggc ccagagccgg ggattggaca
     1021 ccagccgctt catgtaccag atgccgaaga caatccccac gatataggcc agcgcatacc
     1081 agcgtatggc gaaaggcccg attgaaaaca gcaccggatc aatattgggg aatggcagca
     1141 ccgcaaaagt gaacatggcg ccggacagtg tctgcgtcgc gccctgccgt caagtccgca
     1201 gaaaaagctt gatcacgccc cgtgcagaag gtcggtcgaa cctttggccg cagcatccgg
     1261 gtgacccaag ccgcgcaact gtccgagtgc tgattgcacc acaaagcggg ccccgcgcca
     1321 gccgcgcagg cgcaaagacg tgccggtcga aacccggcgc agccacaggg ccttttgcag
     1381 ggactcgact tcgcactgca cctttaccgg atcagcatag aaatccgcca catcggcggc
     1441 ttcaattgtt tctttgccga gccacgcggg cacataggtg gtgcacaggc gctggacatc
     1501 ggtcaggact ttattgaccc ccgtgcccgc cgccggacag gaggttgaat aggcgtcacc
     1561 cacaagcacg attcctggtc tttccggtgc ctttacccgc acgagatcga ctggccggat
     1621 gctgacttgg ccgtcgatgg tgaacggccc agtgaattgc gcaagcgtcg gaataaggtc
     1681 gaacaatgcc tccctgggct gatggcggaa tttccgcagg cgcgggtctt ccatgttcca
     1741 gtagatgaaa agattggccc gcatggcgcc cggcactgga aacactgtga gataggccat
     1801 ggattggtcg ggccgttccg gaaagaccgt tagtgcggga aacgcgaagg gcgtgtcacc
     1861 ctcgggcttc atgtcaaatc cgatgctgat cgagtggcag gggctgagca cctcgcgctt
     1921 gatctcaagg gtttcacgca agccgggatt cagtccattg gccagtatca ccagcctggc
     1981 ctcgatctca tcaccattgg caaggctaac acgctgcctc ccggcattgc cggaaattgc
     2041 cgagactttt gctgcgatat gcgggacact ggcgggaatt tgctgtctca ccgtgttcac
     2101 cagatcatga tagtgaatgc cggccgggct gtttttattg cggtcaacca cccgcccata
     2161 gcgcaccacc cacacttttc caccggcaca ggtgtggggc agaacatcat cagcaagtcc
     2221 cgtgcgggag agcagttcca gctgcgagtc gtcaagcttc tcgcagcgga attccgggcg
     2281 ctgctccgcg tgcggttcaa tgaggatcgc cgcatgcccg gcgcggccga gcatggccgc
     2341 agcggtggcc ccggcaagcc cgccgccgat gattgccaca tctgtcgcga gattattcac
     2401 ccggcgtatt ccctgacatc atctgggcgg cgaaccgcgc agcgcggagc agtgtgtccg
     2461 actttttcac cttgcctttg acggcaaggc cggcacgcca gagcggtgct gccacctgtc
     2521 ccgccgccgt aatcggaatg acggagtcgt aaagcgtttc agcatcccgg caatagacct
     2581 gcttgtaggc cgcatcgccg atgccgagat cgaatttctg aaggccgcgc tcgcagcaca
     2641 tccggatgac attggcgagc agcagttcgc cgggactgta gtttctgaac tccgccccgg
     2701 cgatcgaatt gaacatcccg ctgaagcgcc cgtcggccac gatgccgcca aaggtggcga
     2761 tgattttgcc gccaacccag agagcataga gttcgatgac cggctcgccc gtatcgggat
     2821 caattccatg ggccgcggcc cgggtgaatt ccttcacgcc agccgcatcg aaggcattgg
     2881 atagcccctg ttcgcgcatg cgcgccgcct tctgcgcaaa gaaggccgca agcacctggt
     2941 cgatttccgc ggaggtccgg gcgcgctgaa agccgaca
//
LOCUS       DAORVN010000212         2968 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_29955_length_2968_cov_4.039821, whole genome shotgun sequence.
ACCESSION   DAORVN010000212 DAORVN010000000
VERSION     DAORVN010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2968)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2968)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2968
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29955_length_2968_cov_4.039821"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..106)
                     /gene="obgE"
                     /locus_tag="PKV23_05270"
                     /gene_synonym="cgtA"
                     /gene_synonym="obg"
                     /gene_synonym="yhbZ"
     CDS             complement(<1..106)
                     /gene="obgE"
                     /locus_tag="PKV23_05270"
                     /gene_synonym="cgtA"
                     /gene_synonym="obg"
                     /gene_synonym="yhbZ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012333953.1"
                     /note="ObgE; essential GTPase; exhibits high exchange rate
                     for GTP/GDP; associates with 50S ribosomal subunit;
                     involved in regulation of chromosomal replication; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HQX84880.1"
                     /translation="MKFLDEAKIYVRSGDGGAGAVSFRREKFIEMGGPD"
     gene            302..814
                     /locus_tag="PKV23_05275"
     CDS             302..814
                     /locus_tag="PKV23_05275"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HQX84881.1"
                     /translation="MKNRLIAGLSAVVFSAAIFTVTTEAGTKSKNSLSTEQVKTSKRQ
                     KSRKAGVTPATTDRVVGLIKDMAPKYGVPTWFALRIAKVESGYNPNSRGAAGEYGVFQ
                     MKCATAKGIGFSGNCSELLNASTNVEVGLKHLQMAMKLSNGNLKMAASKHNGGLGRKR
                     LVHHYVAMVF"
     gene            complement(888..1154)
                     /gene="rpmA"
                     /locus_tag="PKV23_05280"
     CDS             complement(888..1154)
                     /gene="rpmA"
                     /locus_tag="PKV23_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012172613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HQX84882.1"
                     /translation="MAHKKAGGSSRNGRDTAGRRLGVKAFGGEDVIAGNIIIRQRGTT
                     WHPGTGVGLGKDHTIFATVPGKVVFRGTKKNKQIVSVVPQTAAE"
     gene            complement(1161..1628)
                     /gene="rplU"
                     /locus_tag="PKV23_05285"
     CDS             complement(1161..1628)
                     /gene="rplU"
                     /locus_tag="PKV23_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008121909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HQX84883.1"
                     /translation="MYAVIKTGGKQYRVAANDKILIEKLDGAAGDQVNFTEVLMVSNG
                     GTVDIGAPFLSGATVVGEIEKQARGPHIIIFKKRRRKHYRRRNGHRQDLTSVTITEIL
                     TGGAKPTAKAKAKPEAKPEAKPEAAAAPVAEAAAEAKPKKAAAKPRKPKTEKE"
     gene            complement(1818..2600)
                     /locus_tag="PKV23_05290"
     CDS             complement(1818..2600)
                     /locus_tag="PKV23_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008127810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HQX84884.1"
                     /translation="MPDTGDARIKLNLEGNLAIITIARPEKLNALDYDMVQALERAAH
                     MVDAMEAIRCCIITGEGEKSFCAGGDIEAWSKWSPENFGLAWIRHGHRAFDALARLRV
                     PLIAALNGHTLGGGLELAATADFRVAEPQVNFGLPETGLGIIPGWSGTQRAVRRFGSQ
                     TVRRMALLGEIFSADEALRLGIVDSVAAKGEGLAAAKLMAAKLAIRGPLATAVTKMLI
                     NAAEGEDVERALETLGGLAVADGHELKTGVAAFREKRKPDFR"
     gene            complement(2593..>2968)
                     /locus_tag="PKV23_05295"
     CDS             complement(2593..>2968)
                     /locus_tag="PKV23_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012557384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HQX84885.1"
                     /translation="DITSRARKIVYSGFFNAGAKMEIRDGKLHIEKEGKVKKLVREVE
                     QISFSGKRAVTQGQDITYVTERCVMKLTPEGVVVTEIAPGVELQANILDQAEFPLIVS
                     PKLQPMSRALFEPARMGLKLHA"
BASE COUNT          585 a          821 c          901 g          661 t
ORIGIN      
        1 catcgggacc gcccatttcg atgaattttt cgcgccggaa agacacagca ccggcgccac
       61 cgtcaccgga gcggacatag atttttgctt catcaaggaa tttcatgggg ccttgtaacc
      121 gtttgtgggc gccaatccta gagcatatcg cagtgcagca ttatttggtg cgacgccaca
      181 taatggtcac attttcccag tttaccggac atcttcccca acattgataa tcattaaaat
      241 aaaaggagcc agtctatcca agtttagttt tttggtgcgg ttataaaact taaggataaa
      301 aatgaaaaat agactcattg caggactttc ggccgttgta ttttctgcgg ctatattcac
      361 agtaacaaca gaagctggca caaaatccaa aaatagtctt tccacggagc aagtcaaaac
      421 gtcgaaacgc cagaagagcc gcaaggcagg ggttacaccg gcaacgacgg accgggtggt
      481 gggcctgatc aaggacatgg cgccgaaata cggggtgccg acctggttcg ccttgcgcat
      541 cgccaaagtt gaatcgggtt acaatcccaa cagccgtgga gccgccgggg aatacggcgt
      601 ctttcaaatg aaatgcgcca ccgccaaggg catcgggttc tccggcaatt gctccgaact
      661 gctcaatgct tccaccaatg tcgaggtcgg cctcaagcat ttgcagatgg cgatgaagct
      721 ttcgaacggc aacctcaaaa tggcggcctc aaagcacaat ggcgggcttg gccgcaagag
      781 gctggttcat cactacgtcg caatggtttt ctaagcccgg caggtcatgc gtaaaacaaa
      841 aggagaggca atcgcctctc ctttttcaat tgggcctgaa gggctggtta ctcagcggct
      901 gtctgcggga cgaccgagac aatctgcttg ttcttcttgg tgccgcgaaa cacaaccttg
      961 ccggggacgg ttgcaaagat agtgtggtcc ttgccgaggc cgacgccggt gccgggatgc
     1021 caggtggtgc cgcgctggcg aatgatgatg ttgccagcga tgacgtcttc gccgccgaaa
     1081 gccttcacgc caagacgccg gcctgcggta tcgcggccgt tgcgggatga gccgcctgct
     1141 tttttatggg ccatggggtg ttactccttt tccgtcttgg gcttgcgcgg cttggcggca
     1201 gccttcttgg gctttgcctc ggctgcggct tctgcaaccg gcgcggcggc agcttccggt
     1261 ttggcctcag gcttggcttc gggttttgcc ttggccttgg ccgttggctt tgctccgccg
     1321 gtcagaatct cggtaatggt cacactggtc aagtcctgac ggtggccatt gcggcggcgg
     1381 taatgcttgc gccggcgctt cttgaaaatg atgatgtggg gcccgcgggc ctgcttttcg
     1441 atctcaccga caacggtggc accggacagg aagggggcgc cgatatccac ggttccgcca
     1501 ttggagacca tgaggacctc ggtgaaattc acctggtcgc cggccgcgcc gtcgagcttt
     1561 tcgatgagga ttttatcatt ggcagcaaca cggtattgtt tgccgcccgt cttgatgact
     1621 gcgtacattt gatagccttt ttcattcccc gcgccctttg gcgccgctgc aaactcatta
     1681 aacagcgggc tttagtgcct gatattacag gctttttcga gttgccggag gcagcgcatg
     1741 gacgcatggc ggagttgccc ccgacagcgc tgggcggttt ctagggaaag ggtgcggaaa
     1801 tgtcaaggaa agggcattta cctgaagtcg ggcttgcgct tctcccggaa ggccgcaacc
     1861 ccggttttga gttcatggcc atccgcaaca gccagacccc caagagtctc cagggcacgt
     1921 tcgacgtctt cgccttcagc ggcgttgatc agcatcttgg tgaccgcggt ggcaagaggg
     1981 ccgcggatgg cgagtttggc tgccatgagc ttggccgcgg cgaggccttc gcccttggcc
     2041 gcaacggaat cgacaatacc aagccgcagg gcctcatcgg ccgagaagat ttcccccaga
     2101 agcgccatgc ggcggacggt ttgcgagccg aagcggcgca cggcgcgctg ggtgccggac
     2161 cagccgggaa taatgccaag gccggtttcc ggaaggccga agttcacttg gggctcggcc
     2221 acgcggaaat cggctgtcgc cgcgagttcc agcccgccgc ccagggtgtg gccattgagc
     2281 gcggcgatga gcggcacgcg caacctggcc agggcatcaa acgcgcggtg gccgtggcgg
     2341 atccaggcga ggccgaaatt ctctggcgac cacttgctcc aggcttcaat gtcgccgccg
     2401 gcgcagaagg atttttcgcc ttcgccggtg atgatgcagc agcggattgc ttccatggca
     2461 tcgaccatat gggcggcgcg ttccagggcc tgcaccatgt catagtcgag cgcgttgagt
     2521 ttttcaggcc gggcgatggt gatgatggcc agattgcctt cgaggttcag tttgatgcgc
     2581 gcatcgccgg tgtcaggcat gaagtttcaa tcccattctg gcaggctcga acagggcgcg
     2641 gctcatgggt tgcagcttag gcgaaacgat gagcggaaat tcggcctgat cgagaatgtt
     2701 ggcttgcagt tcaacacccg gcgcaatctc ggtcaccacc acgccttcgg gcgtgagctt
     2761 catgacacag cgttcggtga cataggttat gtcctggccc tgggtgacgg cgcgtttgcc
     2821 ggagaaggaa atctgctcca cttcgcgcac cagtttcttg accttgcctt ccttttctat
     2881 gtgcagcttg ccgtcgcgga tttccatttt tgcgccggca ttgaaaaaac cggagtagac
     2941 gatttttctg gcgcgcgagg tgatgtcg
//
LOCUS       DAORVN010000213         2951 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_30237_length_2951_cov_3.469959, whole genome shotgun sequence.
ACCESSION   DAORVN010000213 DAORVN010000000
VERSION     DAORVN010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2951
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30237_length_2951_cov_3.469959"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1997
                     /locus_tag="PKV23_05300"
     CDS             <1..1997
                     /locus_tag="PKV23_05300"
                     /inference="COORDINATES: protein motif:HMM:NF015649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HQX84886.1"
                     /translation="IAMTISAKKNRALLAVLALSPGMAATRERLAGLLWGDRPDEQAR
                     SSLRQSLAALRKELDDADEAILQVKDDTVRLCAATVGVDVAAFLKCGQSDDLESLREA
                     ARLYRSELLSDFGSRDEGFEAWLSIERAQLRQEAIRVLEKLAGLVGGGERLAVAQKLV
                     SLDPLREASQRLLMHSQCCLGERGLALKQFDSLKSLLRDELGVEPAPETQHLANQIAD
                     GTIALSGSAAPLSVIASAKPVVAVLPFSNMSGDPEQDYFADGMTEDIITDLSRFSGIS
                     VIARNTVFAYKGKTPNVREVCHRLNACAVLEGSIRKSGQRVRITAQLTDGVSGTHLWA
                     DRYDRELTDIFTLQDEIARTIVEQLRIKLLPGELAAEHAPGVKFDAYNCYLKGRQFFH
                     VGTRSSLKLARRLFECATKCESGPRYAKAFAGIALADARLRAWHGDDIPVAEILAAAT
                     AARELDPGLAEAQVAFGIAASQDGRSSEATAAFEKAIELDPDSYDAHFFYAWHSLANG
                     GIAKSADLYRRATELQPDDFRSPIYLRSVFHALGDESEARRFSLLGLERAEKAFRLHP
                     DSPDAAQLGACVLASLGECDKAKEWLSHSLAIDPDGMLSKYNSACVFAMLGESEAAVA
                     LLADWLKRADRHARRWFLIDADLVSLQQHPRYRELLEIAS"
     gene            2136..2630
                     /locus_tag="PKV23_05305"
     CDS             2136..2630
                     /locus_tag="PKV23_05305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX84887.1"
                     /translation="MNRALIAAALSVALLVPAAQAAQPKFFDNLSGSWSGSGQAYVKK
                     YGDISANCRVAIKGADSKIAMDGTCGMLVFRQALGLSLKNVGGNKYVGTYTGSKTGPA
                     RLEGTLTGDRLVMTIKWGGLVNGDRTAQMVLQRTGPNSFAQIVSDTVEGKKRSTSNFA
                     FVRR"
     gene            complement(2639..>2951)
                     /locus_tag="PKV23_05310"
     CDS             complement(2639..>2951)
                     /locus_tag="PKV23_05310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HQX84888.1"
                     /translation="AGDEGTAKPVGFFGATFFQWVNPKGWLVSIGAAGTYLQPDAGSQ
                     LLQALVFAGLLFITAIPSGFVWLVLGASLQGLLHDARSARLFNTVMALALAASVLMMF
                     W"
BASE COUNT          571 a          947 c          895 g          538 t
ORIGIN      
        1 gcatcgcaat gacgatttcc gccaaaaaga accgggcgct gctggcggtt ctggccctat
       61 cacccggcat ggcggcaacc cgtgagcgcc tggcgggcct cctctggggc gaccggcccg
      121 acgaacaggc ccgcagcagc ctgaggcaat cgctggcggc gctccgcaag gaactggatg
      181 atgccgatga agcaatcctt caggtcaagg atgatacggt gcgcctgtgc gccgcaacgg
      241 ttggcgttga tgttgccgcg ttcctgaaat gcgggcagtc cgacgacctc gaatccttgc
      301 gcgaggcagc gcggctttac cgcagcgaac tgctttcgga ttttggcagc cgcgacgagg
      361 gcttcgaagc ctggctttcc atcgagcggg cgcaactgcg ccaggaggcg atccgcgtcc
      421 ttgaaaaact ggccggcctg gtgggcggcg gcgagagact ggcggtggcc cagaagctgg
      481 tgtcgctgga tccgctgcgc gaagcctcgc agcgcctgct catgcacagc cagtgttgcc
      541 tcggtgaacg cggactggcg ctcaagcaat tcgacagcct gaagtccctg ctgcgggatg
      601 aactcggcgt tgagccggcc ccggaaaccc agcaccttgc aaaccagatt gccgatggca
      661 ccatcgccct gtccggcagc gccgcgccgc tctccgtgat agccagcgcc aagccggttg
      721 tcgccgtgct gccgttcagc aatatgagcg gcgatccgga acaggattat tttgccgacg
      781 gcatgaccga ggatatcatc accgacctct cgcggttctc cggcatcagt gtgatcgcgc
      841 gcaacacggt gtttgcctac aagggcaaga cgccgaacgt ccgcgaggtc tgccaccggc
      901 tcaatgcctg cgcggttctc gaaggcagca tccggaaatc cggacagcgg gtgagaatta
      961 ccgcgcagct caccgatggc gtttccggga cccatctctg ggccgatcgc tatgaccggg
     1021 agctcaccga catcttcacc ctgcaggatg aaatcgcccg gaccattgtc gaacagcttc
     1081 gcatcaaact gctgcccggg gagctggccg cagaacatgc gccgggcgtg aaattcgatg
     1141 cctataactg ctatctcaag gggcgccagt tcttccatgt cggaaccagg tcgtcgctca
     1201 aactcgcccg ccgcctgttt gagtgcgcga ccaagtgcga aagcgggccc aggtacgcaa
     1261 aggcgtttgc tggaattgcc ttggccgatg cgcggttgcg cgcctggcat ggtgacgaca
     1321 tacccgttgc cgagattctc gctgctgcga ccgcggcacg ggaacttgat cccggcctcg
     1381 ccgaagccca ggtcgccttt ggcatcgcgg cgtcgcagga tggcaggtcc agcgaagcca
     1441 ccgccgcctt tgagaaggcc attgagctcg atccggattc ctatgacgcg cacttttttt
     1501 atgcctggca cagcctggca aacggcggca ttgcgaagtc cgccgacctg tatcgccgcg
     1561 ccactgaatt gcagcccgat gacttccgct cgcctattta tctgcgcagc gtcttccatg
     1621 cccttggcga tgaatcggag gcaaggagat tttccctgct cggcctggaa agggcggaga
     1681 aggcattccg cctccacccc gacagcccgg atgcggcgca actcggggcc tgtgtcctgg
     1741 cgtccctggg tgaatgcgac aaggccaagg aatggctttc gcacagcctg gccattgacc
     1801 ctgacggcat gctctcgaaa tacaactcgg cctgcgtctt cgccatgctg ggcgaaagcg
     1861 aagcggcagt cgccttgctg gccgactggc tgaagcgggc ggaccgccat gccaggcgct
     1921 ggttcctgat tgatgccgac ctggtatcgc tgcagcagca cccgcgttac cgggaattgc
     1981 tggaaatcgc ctcgtgatcc ggccccgggg cccaaaaaaa cctgcatttt gagccgttaa
     2041 ccgtgtttgg gtgagggaac ataagtgccg caaagccgtt gcattcttgc cgcaatggct
     2101 ctctatgcgg atgttttttc atgaaggtat tactgatgaa ccgtgccctt atcgccgccg
     2161 ccctgtccgt tgccctgctg gtccctgctg cccaggctgc ccagccgaaa ttctttgaca
     2221 atctttccgg cagctggtcc ggctccggcc aggcctatgt gaagaaatat ggcgacatat
     2281 ctgccaattg ccgggtggcc atcaaaggcg ccgacagcaa aatcgccatg gacggcacct
     2341 gcggcatgct ggtgttccgc caggcgctgg ggctttccct gaagaacgtc ggcggcaaca
     2401 agtatgtggg cacctatacc ggctccaaga ccggccctgc caggcttgaa ggcaccctga
     2461 ccggcgaccg cctggtgatg accatcaaat ggggcggcct ggtgaacggc gaccgcaccg
     2521 cccagatggt gctccagcgc accgggccaa actccttcgc ccagatcgtc agtgacacgg
     2581 ttgaaggaaa aaagcgcagc acctcgaact tcgccttcgt gcgccgctga aacatcctct
     2641 accagaacat catcagcacc gaggcagcaa gcgccagcgc catcacggtg ttgaataggc
     2701 gcgccgacct tgcatcatgc agcagcccct gcagggaagc gccgagcacc agccagacaa
     2761 agccgctggg aatcgccgtg atgaagagca atccggcaaa cacgagggcc tgcagcagtt
     2821 ggctgcccgc atcgggctgg agataggttc ccgccgcgcc gattgacacc agccagccct
     2881 tgggattcac ccactggaaa aacgtcgcgc cgaaaaatcc cacgggcttt gccgtgccct
     2941 catcgccagc c
//
LOCUS       DAORVN010000214         2945 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_30355_length_2945_cov_2.831488, whole genome shotgun sequence.
ACCESSION   DAORVN010000214 DAORVN010000000
VERSION     DAORVN010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2945)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2945)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2945
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30355_length_2945_cov_2.831488"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>363)
                     /locus_tag="PKV23_05315"
     CDS             complement(<1..>363)
                     /locus_tag="PKV23_05315"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HQX84889.1"
                     /translation="VASGEAKIVVGARSALFLPWKKLGLIVVDEEHESAYKQDDGVTY
                     HARDMAVLYGSIGKFPIILSSATPSLESIVNADRGRYGHVKLNDRHGRPELPEIDLVD
                     MRKTPLEPGAWLSAPCIEN"
     assembly_gap    364..373
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<374..1236)
                     /locus_tag="PKV23_05320"
     CDS             complement(<374..1236)
                     /locus_tag="PKV23_05320"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HQX84890.1"
                     /translation="MNSDSRPIADVLLPLALEGPYSYRIPAGMVLEEGCYVAVPLGPR
                     SLIGVVWALKTQAPEGTKLRDVIARFDMPPMAGIHRKFVDWLADYYVEPKGNVLRMVL
                     RCPGAFEEAKGQVAYRATGRTIRRMTPQRQRVLDVAAEGFAMRLSELAKSAGVGTSVV
                     KSMAKEGALEVVALPAHKAFQLPNLNAGGLTLSKQQQLAAQELRAVVTSRSTRVMLFD
                     GVTGSGKTEVYFEAMAAALAAGGQVLLLLPEIALTAGFITRVETRFACEPAQWHSDLR
                     PRERERVWRGVA"
     gene            1291..2655
                     /gene="pabB"
                     /locus_tag="PKV23_05325"
     CDS             1291..2655
                     /gene="pabB"
                     /locus_tag="PKV23_05325"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014497768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008153 - para-aminobenzoic acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HQX84891.1"
                     /translation="MKHTTVQPTQVHAREIVYWEPIQLAQRLRGQAFLTLFESTMRQE
                     KLGRYSFLACNPASTLKVENGRTLLDGVVQEEAPLAVMDKILSRNRLPKLAGLPPFQG
                     GLAGYIGYDFGRSLEPHTRLPEFPALCPELMLHVYDVGIAFDHLQERAWIISTAGEPP
                     ADELEELLKRKAQGLGSPAIENWSSNFTRESYEAAVTRTIEYILAGDIFQANISQCFS
                     AAIPEGFEALAFYKRLRSLNPATFSAYLDYGDVQIASSSPERLLSFDGMTVEARPIKG
                     TRRRDPNPALDAALVAELSSSRKDRAENVMIVDLLRNDLSRVCAPGSVRVPVLCGLES
                     YASVHHLTSVVTGVLKPDKTVGDLIAAVFPGGSITGAPKIRAMEVIAEIEGQGRGIYC
                     GSIGYFGFNGQVDLSIAIRTAMFLQGTARFQGGGGITARSEPAAEYEETLTKISRIME
                     AFAT"
     gene            2652..>2945
                     /locus_tag="PKV23_05330"
     CDS             2652..>2945
                     /locus_tag="PKV23_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017484260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HQX84892.1"
                     /translation="MILIVDNYDSFVYNIARYFEELGQQVVVKRNDAVDEANLKARAI
                     VISPGPCTPHEAGSSLEIVRNFSGKLPILGVCLGHQCIGEVFGGKVSRARRPMH"
BASE COUNT          578 a          805 c          949 g          603 t
ORIGIN      
        1 gttttcaatg cagggggccg acaaccaggc gccgggctca agcggggttt tgcgcatgtc
       61 gacgagatca atctcgggta gttcggggcg gccatgccgg tcgttcagtt tcacatggcc
      121 atagcggccc cggtcggcat tgacgatgga ttcaagcgag ggtgtggccg acgacaggat
      181 aatcgggaat tttccgatgg agccatagag cacggccatg tcgcgggcgt ggtaggtgac
      241 gccgtcgtcc tgcttgtagg cgctttcatg ttcctcatcg acaacgatga ggcccagctt
      301 tttccatggc aggaaaagag cggagcgggc gccgacgaca atttttgcct cgccgctagc
      361 aacnnnnnnn nnngcaacgc cgcgccagac gcgttcgcgt tcgcggggcc gcaggtcgga
      421 atgccattgg gcgggctcgc aggcaaagcg ggtttcaacg cgggtgatga atccagcggt
      481 gagcgcaatt tcgggaagca gcaagagtac ctgcccgccg gcggcgagag ccgccgccat
      541 ggcttcgaaa taaacctcgg tcttgccgga accggtgacg ccatcaaaga gcatgaccct
      601 ggtgctgcgg gaagtgacga cagcgcgaag ctcctgggca gcgagctgtt gttgcttgga
      661 cagcgtgagg ccaccggcgt tcaggttggg caattggaaa gctttatgcg cgggaagcgc
      721 cacgacttcc agggcccctt ccttggccat ggattttacc acagaggtgc caacgccggc
      781 ggacttggcc agttccgaca ggcgcatggc aaagccttca gcggccacat caaggacgcg
      841 ctggcgttgg ggcgtcatgc ggcggattgt gcggccggtg gcgcggtagg caacttgccc
      901 ctttgcttcc tcaaaggcgc cggggcagcg cagcaccatg cgcagcacat tgcccttagg
      961 ctcgacgtaa tagtcggcca gccaatccac aaatttgcgg tggattccgg ccatgggcgg
     1021 catgtcgaaa cgggcgataa catcgcggag cttggtgcct tccggcgcct gggttttcag
     1081 agcccagacg acgccgatga ggctgcgggg gccaagcggc acggcgacat agcaaccttc
     1141 ctccagcacc attccggcgg gaatccggta ggaatagggg ccctccaggg ccagcggcaa
     1201 gagcacatcg gcgataggtc gggaatcgga attcatggct ctggaatagt cttggacggg
     1261 agggcctgcc agtcttatat cacgcgtgcc atgaaacata cgactgttca gccgacacag
     1321 gttcatgccc gcgagatcgt ttactgggag cccatccagc tggcccagcg cttgcggggg
     1381 caggcctttc tcacgctgtt tgaaagcacc atgcggcagg aaaaactggg gcgctattcg
     1441 tttctggcct gcaacccggc atctaccctg aaggtcgaga atggccggac cctgcttgac
     1501 ggtgttgtcc aggaagaggc gccgcttgcc gtgatggata aaatactcag ccgcaaccgc
     1561 ctgcccaagt tggcgggact gccgccgttc caaggcgggc ttgcaggtta tatcggctat
     1621 gactttggcc gtagccttga gccccatacg cgcctgcctg aatttcccgc gctttgcccg
     1681 gaactcatgc tgcatgtcta tgatgtgggc attgcctttg accatttaca ggagcgggcc
     1741 tggatcattt caacggcagg tgagccgcct gccgatgagc tcgaagaatt gctgaagcgc
     1801 aaggcgcaag gcctggggtc accggcaatc gaaaactggt cctccaattt cacccgtgag
     1861 tcctatgaag ctgcggtaac gcggacgata gaatatattc tggcggggga catctttcag
     1921 gccaatattt cgcagtgctt cagtgcggca attcctgagg gctttgaagc attggcgttt
     1981 tacaagcgcc tgcgctccct gaatcccgca accttctcgg cctatctgga ttacggcgat
     2041 gtgcagattg cctcaagctc gccggagcgg ttgctgagtt ttgatggcat gacggttgag
     2101 gcacgaccga tcaagggcac gcggcggcgt gatcccaatc cggccctgga cgcggcactg
     2161 gtggcggaac tttcgtcaag ccgaaaggac cgggcagaaa atgtgatgat tgtggatctg
     2221 ctgcgcaatg acctgagccg cgtctgtgcg cctggcagcg tgcgggtgcc ggtgctgtgc
     2281 ggcttggaga gctatgcgtc tgtgcatcat ctgacttccg tggtaaccgg tgtattgaag
     2341 ccggacaaga cggtgggaga cctcatcgcg gcagtgtttc caggcggctc gattacgggc
     2401 gcgccgaaaa tccgcgccat ggaagtgatt gcggagatcg aggggcaggg gcgcgggatc
     2461 tattgcggct ccattggcta tttcggcttc aacgggcagg tggatttgag catcgccatc
     2521 cgtaccgcga tgtttttaca aggcacggcg cgtttccaag gcggcggtgg tatcacggcc
     2581 cgttcggaac cggcggcgga atacgaagaa acactcacca agatcagccg catcatggag
     2641 gcctttgcga catgatcctg atagtcgata actacgacag ctttgtttac aacattgcgc
     2701 gctatttcga agagcttggc cagcaggttg tggtgaagcg caatgatgcg gtggatgagg
     2761 ccaatctcaa agcccgggcc attgtgattt ctccgggccc ctgcacgccg catgaagcgg
     2821 gcagttcatt ggagattgtg cgaaactttt ctggaaagct tcccattttg ggcgtgtgcc
     2881 ttggtcatca gtgtattggt gaagtttttg gcggcaaggt cagccgcgct aggcgcccga
     2941 tgcac
//
LOCUS       DAORVN010000215         2940 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_30454_length_2940_cov_2.693241, whole genome shotgun sequence.
ACCESSION   DAORVN010000215 DAORVN010000000
VERSION     DAORVN010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2940
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30454_length_2940_cov_2.693241"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(5..799)
                     /locus_tag="PKV23_05335"
     CDS             complement(5..799)
                     /locus_tag="PKV23_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015666975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQX84893.1"
                     /translation="MTVTLTILGCGSSGGVPRIGGDWGLCNPANPRNRRRRCSILLEM
                     TGASGVTRVLIDTSPDLREQMLSAGVGEIDAVCFSHEHADHTHGIDELRGFYLRQRRR
                     VAIWADEPTMAMLKRRFAYCFETAAGSDYPPVVVANMIEPGREVPISGAGGTIILAPF
                     LVQHGHIEALGFRVGNTAYTPDLNGIPEASIPYVSGLDLWIVDALKRSPHPSHFCLDD
                     TLRAIDRFKPRRAVLTNMHNDMDYDTLRAELPGGVEPAYDGLKLPL"
     gene            complement(796..1596)
                     /locus_tag="PKV23_05340"
     CDS             complement(796..1596)
                     /locus_tag="PKV23_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018697951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HQX84894.1"
                     /translation="MSLLVVDSHCHLDYEGLTERLPEVLANAEAAGVGLMVSIGTQVK
                     NFERLRHIAEDNSNVFCTVGTHPHHAHEELDIEVAELVKLARHPKVVGIGEAGLDYHY
                     DLSPRDAQAQGFRVHIAAARETGLPLVIHAREADADVAGILEDEMKKGAFKPLLHCFT
                     SSLELAQRGLAIGAYVSFSGILTYKSAENLRAVAAEVPMDRLLVETDSPYLAPVPHRG
                     KSNEPAFVIKTLEQLAAVKGVTPEAMAKVTNDNFFRLFSKVPRPARFA"
     gene            complement(1593..>2940)
                     /gene="metG"
                     /locus_tag="PKV23_05345"
     CDS             complement(1593..>2940)
                     /gene="metG"
                     /locus_tag="PKV23_05345"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HQX84895.1"
                     /translation="PLALADRTAAQFEEMGELLNARADDIVRTTSARHRAAVQDIWRR
                     MEANGDIYLSKYSGWYSVRDEAYYDEGEIEDREGKKYSTKTGTPVEWVEEESYFFKLS
                     AYAKPLLDHYEAHPDFIVPEKYKNEIVAFVKRGLNDLSISRTTFDWGIPVPGNPKHVM
                     YVWVDALTNYITSTGYPDAKAARAKFWPASAHVIGKDITRFHAIYWPAFLMSAKLPVP
                     KQIVVHGFLFSRGEKMSKSVGNVVAPADLVTNYGVDPVRYFFMREVPFGQDGNYSHEA
                     IVARMNADLSNDLGNLAQRSLSMIAKNCDGKVPACGALTDADKEILTSADALLAQVRV
                     HHADFAISRALDTIWKVVADTNRYFAGQEPWALKKTNPARMGTVLYVTAEVLRIVGIL
                     IQPYVPQSAAKLLDLLGVNARDFTDLPKRLASGGALPAPQPIFPRYVEEGEAPAKS"
BASE COUNT          609 a          938 c          810 g          583 t
ORIGIN      
        1 ggaatcacaa cggcagttta agcccgtcat aggccggttc aacgcctccc ggaagctccg
       61 cccgcagcgt atcatagtcc atgtcattgt gcatgttggt gagaaccgcc cgcctcggct
      121 tgaagcggtc aatggcccgc agcgtgtcgt ccaggcagaa gtggctggga tggggactgc
      181 gcttcagggc gtcaacgatc cacaggtcga gaccggaaac atagggaatg ctcgcttcag
      241 gaatgccgtt gaggtctggc gtgtaggccg tgttcccgac gcggaaaccg agcgcctcaa
      301 tatgcccatg ctggaccaga aaaggagcca gtatgatggt tccccctgct ccggaaatag
      361 gcacttcccg gcccggttcg atcatgtttg caaccacgac cggaggataa tcgcttcccg
      421 cagccgtttc gaagcaatag gcaaagcgcc gcttgagcat ggccatcgtc ggctcatccg
      481 cccagatcgc aacccgccgc cgctggcgca aatagaatcc acgcaactca tcaatgccgt
      541 gggtatggtc cgcatgttca tggctgaagc acaccgcatc aatttctcca accccggcgc
      601 taagcatctg ctcgcgcaaa tccggcgagg tgtcgatcag gacccgtgtt acgccggaag
      661 caccggtcat ctccagcaga atggagcagc gccgccgccg gttcctggga ttggcagggt
      721 tgcacaggcc ccagtcaccg ccgatgcgcg gaacgccacc cgaggatcca caacccaata
      781 tggtcagcgt gacagtcatg caaaccgtgc cggtcgcgga accttggaga acagccggaa
      841 gaaattgtca ttcgtcacct tggccatggc ttcaggagtg acacctttga cagcggccag
      901 ttgctcaagt gttttgatca caaaagccgg ctcattggat ttgccgcggt gcggaaccgg
      961 cgccagataa ggcgagtcag tttccaccag cagccggtcc atcggcacct ccgccgcaac
     1021 cgcccgtaaa ttctcggcgc tcttgtatgt gagaatcccg gagaatgaaa cataggcacc
     1081 gattgcaagg ccccgctgcg ccagctcaag cgaagaggtg aaacaatgga gcaatggctt
     1141 gaacgccccc tttttcatct cgtcctcgag aattcccgcc acatccgcat ccgcctcgcg
     1201 cgcgtggata acgagtggca aaccagtctc ccgtgccgct gcaatgtgga cgcgaaatcc
     1261 ctgcgcctgc gcgtcgcgtg ggctcaggtc ataatggtaa tccagtcccg cctcgccgat
     1321 ccccaccacc ttgggatgcc tggcaagttt gacaagctcg gcaacctcga tatcgagttc
     1381 ctcgtgcgcg tggtgcggat gggttcccac cgtgcagaaa acatttgagt tatcctcggc
     1441 gatatgccgc aaccgctcga aattcttcac ctgcgtgccg atagaaacca tcagtcccac
     1501 gcccgccgcc tcggcattag ccagcacttc gggaaggcgt tccgtcaatc cttcatagtc
     1561 aagatggcag tggctatcga caacgagaag gctcaacttt ttgccggggc ttccccctcc
     1621 tccacataac gcgggaagat cggctgggga gccggcagcg ccccgcctga agccagccgc
     1681 ttcggcaggt ccgtgaagtc ccgcgcgttc acgcccagta gatccagcag ctttgccgcc
     1741 gattgcggca catagggttg aatgagaatc ccgacgatgc gcaagacttc cgctgtcaca
     1801 taaagcaccg ttcccatccg cgccggatta gtcttcttca aagcccatgg ctcctgcccc
     1861 gcgaaatagc ggttcgtgtc ggccaccacc ttccagatcg tatccagcgc ccggctgatg
     1921 gcgaagtccg cgtgatgcac gcgcacctgc gccagcagcg catcggccga tgtcagaatt
     1981 tccttgtccg catcggtcaa cgctccacag gccggaacct tgccgtcaca gttcttcgcg
     2041 atcatcgaaa gcgagcgctg cgccagattg cccaggtcat tggaaaggtc cgcattcatc
     2101 cgcgccacga tggcttcatg gctgtaattg ccatcctggc cgaagggcac ctcgcgcatg
     2161 aagaaatagc gcaccggatc gacgccatag ttggtgacga gatcggctgg cgccaccaca
     2221 ttgcctaccg acttcgacat tttctcgccg cggctgaaga ggaagccgtg caccacaatc
     2281 tgcttcggca ccggcagctt tgccgacatc aaaaacgccg gccagtaaat cgcatgaaac
     2341 cgcgtgatat ccttgccgat cacatgggcg ctggccggcc agaatttcgc cctggccgcc
     2401 ttcgcatccg gatagcccgt ggacgtgatg taatttgtga gcgcatccac ccatacatac
     2461 atcacatgct tgggattgcc cggaaccgga atgccccagt caaacgtcgt gcggctgatc
     2521 gaaagatcat tcagcccgcg cttgacgaaa gccacgatct cgttcttgta tttctcgggt
     2581 acgatgaagt caggatgggc ttcatagtga tccagcaacg gcttggcata ggccgagagc
     2641 ttgaagaaat agctttcctc ctccacccat tccaccggcg ttccggtctt ggtggagtat
     2701 ttcttgcctt cgcgatcttc gatctcgcct tcatcgtagt aggcctcatc gcgcaccgaa
     2761 taccagccgg agtatttcga caggtagatg tcgccattgg cctccatgcg cctccagatg
     2821 tcctgcacgg ccgcgcggtg ccgcgccgaa gtggtgcgca cgatatcgtc ggcccgcgcg
     2881 ttcagcagct ctcccatctc ttcgaattgc gctgccgtgc ggtcggccag cgccaggggc
//
LOCUS       DAORVN010000216         2933 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_30595_length_2933_cov_3.074705, whole genome shotgun sequence.
ACCESSION   DAORVN010000216 DAORVN010000000
VERSION     DAORVN010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2933
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30595_length_2933_cov_3.074705"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            97..1344
                     /locus_tag="PKV23_05350"
     CDS             97..1344
                     /locus_tag="PKV23_05350"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HQX84896.1"
                     /translation="MQRFVKNFGACQRGSIAVLYAASAIPVFVAAGSAIDYVRYFSNT
                     TELQAALDSAALAAAATPEASDTERLALAEATFLRNLEGGNLAGEAITRSFDIEDETV
                     VAAADMNMSTSLMQFAGIKAMKLSVGAEVAVPANKNAEIALVLDYSGSMNDSIAGGVK
                     YVAMRNAAKDLISDLQAANPDKVKFALVPFSHHVYGTFDKSYILGQSGSGSWTGCTQD
                     RKYPYNLTDATPTADNNTKWGHPQAPVHISSGCGAYPSQQLIMKPLTNDFSGLKTQLD
                     SMTPYAWTNISLGVEFGFHMLSDNAPFEEGVSYDDEETQKFMVVLTDGAQTEPAFGPG
                     STRTVAQGEANLEALCTNAKAKGITMITIAYDLDDNSTTTRLKNCATDPDTNFFVASS
                     GAAVAGAFDNIKTLITAQVFISK"
     gene            1400..2053
                     /locus_tag="PKV23_05355"
     CDS             1400..2053
                     /locus_tag="PKV23_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006203993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2161 family putative PD-(D/E)XK-type
                     phosphodiesterase"
                     /protein_id="HQX84897.1"
                     /translation="MLETELYLPVKRFLEAQGFAVKAEIGGCDVLAVRGDEPPVIVEL
                     KTSFNLQLLLQGIDRQGVTDTVYLAIPEPKRSLPGDILRLCKRLGLGLLVVNGQWVEA
                     YLDPAAYRPRPAPRRKTVLLREFQRRVGDHNSGGSSKRPLVTAYRQDALRCVKFLKTS
                     GASRVCDIVAQTKVERAASILRRDAYGWFAREARGIYFLSPKGEAAVATFGDVLGVV"
     gene            complement(1987..>2933)
                     /locus_tag="PKV23_05360"
     CDS             complement(1987..>2933)
                     /locus_tag="PKV23_05360"
                     /inference="COORDINATES: protein motif:HMM:NF023330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3422 domain-containing protein"
                     /protein_id="HQX84898.1"
                     /translation="TAQFLKKYFGHEDLAGSRVSHGRATVWTDFKIGADGYTRMLIHD
                     QGLTPLSLGRVSRRIHEIETYRMMALLAFPLALDLQRRIGPLEKSLAETIESMLSTQG
                     IEEDARLLASLSTTARDVEEMSNRSSFRFAAARAYSALVTKRIAELGEERAINYQRIG
                     VFLDRRFSPAMTTCFAVAERIADLARRSERASNLLRTRVDIALEGQNQQLLRSMEART
                     RQQLMLQETVEGISVVAISYYLFNIVAIFANGAAKHFFGEGAAFPDWALVPVILLLVW
                     WGIRRLKRRIRKSHPDIQTTPRTSPKVATAASPLGERK"
BASE COUNT          602 a          904 c          842 g          585 t
ORIGIN      
        1 cgtttggacc gtggcttgct cctttgcgtc catacccccg ggaataccgt tccggattga
       61 gacaatggag caaggaagat gcaagtctgt tcaaaattgc aacgtttcgt aaagaacttt
      121 ggcgcctgcc agaggggttc catcgctgtt ctttatgccg cttcggcaat tcctgtcttc
      181 gtggcggcgg gctcggccat cgattatgtc cgctatttca gcaacaccac ggaattgcag
      241 gccgcccttg attccgccgc cctggcagcc gccgcaactc cggaggcaag cgacactgag
      301 cgccttgccc tggccgaggc aacctttttg agaaacctgg aaggtggcaa ccttgcagga
      361 gaggcaatca cccgcagctt tgatattgag gacgaaacgg tggttgccgc cgccgacatg
      421 aacatgtcaa cttcactcat gcagtttgcc ggcataaagg ccatgaagct ttctgtcggc
      481 gccgaagtcg ccgttcccgc aaacaagaac gccgagattg ccctggttct ggactattca
      541 ggatcgatga acgactccat tgccggaggc gtgaaatatg tggccatgag aaacgccgcc
      601 aaggacctga tcagcgatct tcaggcggca aaccccgaca aggtgaaatt cgccctcgta
      661 cccttctcgc accatgtcta tggaaccttc gacaagtcat atatccttgg ccagtcgggc
      721 agcggctcat ggaccggctg cacccaggac cgcaaatatc cctataacct gaccgatgcc
      781 acgccaaccg ccgataacaa cacaaaatgg ggccatcccc aggcgccggt ccatatctcc
      841 tcgggctgcg gcgcctatcc cagccagcag ctcatcatga agccgttgac caatgatttc
      901 tccggcttga aaacacaact cgacagtatg accccctacg cctggaccaa tatttcactg
      961 ggcgtggaat ttggcttcca catgctctcg gacaacgcgc cattcgagga gggcgtgagc
     1021 tatgacgacg aggaaaccca gaaattcatg gtggttctga ccgacggcgc ccagactgag
     1081 ccggcctttg gcccgggcag cacgcggacc gtggcccagg gcgaggcaaa tcttgaagcc
     1141 ctctgcacca acgccaaggc caagggcatc accatgatca ccattgccta tgatcttgac
     1201 gacaattcca cgaccacccg tttgaagaac tgcgcaaccg accccgacac gaatttcttc
     1261 gtggcgtcga gcggcgcagc cgtggccggt gcctttgaca acatcaagac cctcatcacc
     1321 gcccaggtct tcatcagcaa ataaatcttt tgtaaacgaa tcaatgtagg ttccgggttc
     1381 atgtccggga tctattattt tgcttgaaac tgaactctat ctccccgtga agcgctttct
     1441 cgaagcgcag ggctttgccg tgaaagccga aatcggcggc tgcgatgtgc tggctgtcag
     1501 gggcgatgag ccgccggtga ttgtcgaact gaaaacctcg ttcaatctcc agctcctgct
     1561 ccagggcatt gaccggcagg gcgtgaccga tacggtgtat ctcgccattc cggaaccgaa
     1621 acgctccctt ccgggtgaca tcctgcggct gtgcaagcgc ctgggcctcg gtctgctggt
     1681 cgtgaacggc caatgggtcg aagcctatct tgatcccgct gcctaccggc cccgcccagc
     1741 gccgcgccgc aaaaccgtgc tcctgcgcga gtttcagcgc cgggtgggcg atcacaattc
     1801 cggcggcagc agcaagcgcc ccctggtcac cgcctaccgg caggatgcct tgcgctgcgt
     1861 gaaattcctc aagacttccg gcgcctcacg ggtttgcgac attgtcgccc aaaccaaggt
     1921 tgagcgcgcc gcctcaatcc tcaggcgcga tgcctatggc tggttcgcga gggaagcgcg
     1981 cggcatctat ttcctctcgc ccaaaggcga ggcagcggtt gccaccttcg gggatgtcct
     2041 gggcgttgtc tgaatgtccg gatgggattt ccggatgcgc cgtttgagcc gccggatgcc
     2101 ccaccacacg agcaggagta tgacgggcac gagcgcccaa tcgggaaagg cagcaccttc
     2161 cccgaagaaa tgctttgcag cgccattcgc gaatatggcg acgatgttga aaagataata
     2221 gctgatggcg acaaccgaaa tgccctcaac ggtttcctgc agcatcaact gctgccgggt
     2281 gcgcgcttcc atcgagcgca gcaactgctg gttctggccc tccagcgcaa tgtcaacccg
     2341 ggtgcggagc aggttgctgg cccgttcgct gcgccgcgcc aggtcggcaa tgcgctcggc
     2401 cacggcaaaa caggtggtca tcgcggggct gaagcggcgg tcgagaaaca cgccgatgcg
     2461 ctggtagttg atggcccgct cttcgccaag ctcggcgatg cgcttggtga ccagcgcgct
     2521 gtaggcccgt gccgcggcga atcggaagct gctgcggttc gacatctctt ccacatcccg
     2581 cgccgtggtg ctgaggctgg cgagcagccg ggcgtcctct tcgatgcctt gcgttgacag
     2641 catgctttca atggtttcgg ccagggattt ttccaggggg ccaatgcggc gctgcagatc
     2701 cagcgccagg ggaaaagcca gcagcgccat catccggtag gtttcaattt catggatgcg
     2761 gcgcgaaacg cggccaagcg acaggggtgt gaggccctgg tcatggatca gcatgcgggt
     2821 gtatccgtca gcgccaatct tgaaatcggt ccacaccgtg gcgcggccat ggctgacccg
     2881 cgatcccgcc agatcctcgt gaccaaaata cttcttgaga aattgcgcgg tgg
//
LOCUS       DAORVN010000217         2932 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_30609_length_2932_cov_3.594369, whole genome shotgun sequence.
ACCESSION   DAORVN010000217 DAORVN010000000
VERSION     DAORVN010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2932)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2932)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2932
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30609_length_2932_cov_3.594369"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..899
                     /locus_tag="PKV23_05365"
     CDS             <1..899
                     /locus_tag="PKV23_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002718574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HQX84899.1"
                     /translation="TNARNFAGLNFTTDLLEQGIAYARGHGTKVLLAVNSFATAPRTQ
                     LWFDAIDTAARLGVDAVIVADIGVASYTAERHPQLRLHLSVQAGASSPEAIRYYCSEF
                     NVKRVVLPRILTVTEIAAIHREIPCEIEAFVFGNIGMMSEGRCSLTNYVTGTSTNMDG
                     VCSPANAVHYMDLPDGSKTAELGDFEIDCFAPGEHAGYPTICKGRYLSPVRKEPYYAF
                     EEPVSLNLSSLLPDLIAAGVTALKIEGRQRSRSYVQAVVSAYRRAVDDIVAGRWHDLS
                     GLVALTEGGKETQGAFQTKKWR"
     gene            905..1822
                     /locus_tag="PKV23_05370"
     CDS             905..1822
                     /locus_tag="PKV23_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HQX84900.1"
                     /translation="MTSELTMGPLLFHWPAETRRDFWFRVADEAPVDTVYLGETVCSK
                     REPFFNPHIEEVSERLTRAGKRVVWSSLAQVVTAIDRRASTGIAEFEAADIEANDASL
                     LPLLRRKPHRIGQYLNVYNEATITHLASNGAYHFSLQPELPAETISRLTEAAEAAGAT
                     TEVQVFGRVTLALSARCYHARAHGRTKDNCLFVCESDADGMELQTRSHHKFLAINGIQ
                     TLSHRYLELSAEIPALVAMGVSAFRLSPHSLDMIEVARAYRQLLDGRISTDETFARLA
                     GLGMPQPVMNGFFHRKPGYLHVGAQPELS"
     gene            1833..>2932
                     /locus_tag="PKV23_05375"
     CDS             1833..>2932
                     /locus_tag="PKV23_05375"
                     /inference="COORDINATES: protein motif:HMM:NF017727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HQX84901.1"
                     /translation="MATSAQQIRAWQGLAVLGYGFRPFFLGAAAFAAMAMAVWIGALS
                     GTWSIPSAYGPVEWHAHEFMWGYLPAVIAGFLFTAIPNWTGRLPIVGVPLAILWCFWF
                     AGRLAMFVSGSMPIVLAAAIDLSFLTVLLAVVAREIVAGRNWKNLKVLAVVAILAAGN
                     LVFHIEAITGGSAASGVGVRIGVAAAIMLIAIIGGRIVPSFTRNWLARRGPGALPAQF
                     DWTDQAVLAVTALALLLWTAFPASGGSGAIALLAGALNLFRLARWTGWRCLAEPLVAI
                     LHAGFLFIPAGFLLTGAAALWPEIVPPAAAQHAWMAGAIAVMTLAVMTRASLGHSGKA
                     LAAGKGTTAIYGLAITAVIARVVSGIVPAIPLL"
BASE COUNT          532 a          894 c          840 g          666 t
ORIGIN      
        1 aaaccaatgc ccgcaacttc gccggcttga acttcacgac tgatctgctc gaacagggca
       61 ttgcttatgc acgcgggcac ggcaccaagg tgctgctggc ggtgaattcc tttgccacgg
      121 caccccggac gcagctttgg tttgacgcca tcgatacggc cgcacggctg ggcgtggatg
      181 ccgtcattgt tgcggacatc ggcgtggctt cctacaccgc ggagcgtcat ccgcaattgc
      241 ggctgcatct ttcggtccag gctggcgcct cttcgccaga ggcaatccgc tactattgct
      301 ccgaattcaa tgtgaagcgg gttgttctcc ctcgcattct caccgtcacc gagatcgccg
      361 ccattcaccg cgagatccct tgcgagatcg aagccttcgt atttggaaat atcggcatga
      421 tgtcggaagg gcggtgcagc ctgaccaact atgtgactgg cacctcaacc aatatggatg
      481 gcgtgtgttc accggcaaat gcggtccatt acatggatct tcccgatggc agcaagaccg
      541 ccgagcttgg tgattttgag atcgactgct ttgcccccgg cgaacacgca ggctatccga
      601 cgatctgcaa aggccgctat ctttccccgg tgcggaagga gccctattat gcatttgaag
      661 agccagtcag tctcaatctc tcgtctctgc tgccagatct cattgcggct ggagtcacgg
      721 cattgaaaat tgaaggccgc cagcgctcac gttcctatgt acaagccgtc gtttcagcct
      781 accgccgtgc cgttgacgat atcgtggcag gccgctggca cgatctctcg ggacttgtgg
      841 cgctcactga aggcggcaag gaaacccaag gcgcattcca gaccaagaag tggagatagc
      901 agcagtgacc tccgaactca ccatgggccc gctgctgttt cattggccag cagaaaccag
      961 gcgcgatttc tggttccgcg ttgctgacga agcgccggtt gatacagtct atctcggtga
     1021 aaccgtgtgc tcgaaacgcg agccgttctt caatcctcat attgaagaag tgtctgaacg
     1081 cctgacgcgg gctggcaagc gcgttgtctg gtcatcgttg gcgcaagtcg tcaccgctat
     1141 tgaccgcagg gcctcaaccg gtatcgccga attcgaggct gcggacatcg aagccaatga
     1201 tgcatccctc ctcccgttgc ttcgtcgcaa gccgcatcgc atcggccagt atctgaacgt
     1261 ctacaacgag gccacgatca cccatctcgc ctccaatggc gcctatcatt tcagcctgca
     1321 gccggaactt ccggcagaaa cgatctcccg cttgactgaa gcagcggaag cggctggcgc
     1381 cacgaccgaa gtacaggtat ttggccgtgt cactttggcc ctgtcggcgc gctgctatca
     1441 cgcccgcgcc catggccgca ccaaggacaa ttgccttttc gtttgcgaga gcgacgcaga
     1501 tggcatggaa ctgcaaaccc gttcgcatca caaattcctg gcaatcaacg gcatccaaac
     1561 tttgtcgcac cgctatcttg agctttcggc cgaaattccc gcacttgtcg ccatgggcgt
     1621 ctccgccttt cgcctctcgc cccatagcct cgacatgata gaagtcgcac gtgcctacag
     1681 acagttgctc gatggccgga tttccacaga tgagaccttt gcaaggttgg ccggattggg
     1741 aatgccgcaa ccggtaatga atggtttctt ccaccgcaaa cctggctacc ttcatgtggg
     1801 cgcgcaaccc gaattgagct gaggaggcga aagtggccac atcagcgcag cagattcggg
     1861 cctggcaggg gcttgcggtt cttggttacg gcttccgccc gttttttctg ggagcagccg
     1921 ctttcgctgc tatggctatg gccgtttgga tcggagcgct ttcgggcacc tggagcattc
     1981 catccgctta tggcccggtg gaatggcatg cccatgagtt catgtgggga tatcttcccg
     2041 cggttattgc cggctttctc ttcaccgcaa ttccaaattg gaccggccgc ctgccgattg
     2101 ttggcgtgcc tctggcaatc ctttggtgtt tctggttcgc cggacgcctc gccatgttcg
     2161 tctccggctc tatgccaatt gtgcttgcgg cggcgatcga tctgtcattc ctgacggtgc
     2221 tgctggctgt cgtggccagg gaaatcgtag cgggccgcaa ctggaaaaat cttaaagtgc
     2281 tggccgttgt cgcgattctg gcagcaggca atctcgtgtt ccacatcgag gccatcaccg
     2341 gcggcagcgc ggcaagtggc gtcggcgtcc gcatcggcgt ggccgcagcc atcatgttga
     2401 ttgcaattat tggcggcagg attgtgccca gtttcacgcg gaattggctt gccaggcgtg
     2461 gtccaggcgc gctgccagcc cagtttgatt ggacagacca ggcagtcctt gccgtgaccg
     2521 ccctggctct tctcctatgg accgcgtttc cggcgtccgg cggttccggc gccattgcac
     2581 tgctggccgg tgcattgaat ctgttcaggc tggcgcgctg gaccgggtgg cgctgccttg
     2641 cggaaccgct tgtcgccatt cttcatgcgg ggttcctttt catacctgcg ggattcctgc
     2701 tgaccggggc agccgcactt tggcccgaaa tcgttccgcc cgccgccgcc caacacgcct
     2761 ggatggcggg cgcaatagcg gtcatgacgc tcgcggtgat gacccgggca agcctgggtc
     2821 actcgggcaa ggcgcttgcc gcaggaaaag gtacaacggc aatctatggc cttgcaatca
     2881 ctgccgtcat tgcgcgcgtc gtatccggaa ttgttccggc catccccttg ct
//
LOCUS       DAORVN010000218         2929 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_30670_length_2929_cov_2.399095, whole genome shotgun sequence.
ACCESSION   DAORVN010000218 DAORVN010000000
VERSION     DAORVN010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2929
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30670_length_2929_cov_2.399095"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1014)
                     /gene="sucA"
                     /locus_tag="PKV23_05380"
     CDS             complement(<1..1014)
                     /gene="sucA"
                     /locus_tag="PKV23_05380"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018388038.1"
                     /note="SucA; E1 component of the oxoglutarate
                     dehydrogenase complex which catalyzes the formation of
                     succinyl-CoA from 2-oxoglutarate; SucA catalyzes the
                     reaction of 2-oxoglutarate with dihydrolipoamide
                     succinyltransferase-lipoate to form dihydrolipoamide
                     succinyltransferase-succinyldihydrolipoate and carbon
                     dioxide; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HQX84902.1"
                     /translation="MNKTDLIAAFEQSSFLYGGNAQFIEGLYAKYLENPAAVDVHWRQ
                     FFAGLDDDPASAKQQVSGPSWARKDWPLAATGDLVSAFDGNWPAVEKAVGAKIEAKSK
                     AADAKLSVDEVRKATMDSVRALMMIRAFRMRGHLAADLDPLGLAERPAQPELDPSTYG
                     FSEADLDRPIFLDKVLGLEQATIRQITDILKRTYCHTLGVEFMHISDPLQKGWLQQRI
                     EGADKEISFTREGKKAILRKLIEGEGFENFLNVKYTGTKRFGLDGGESMIPALEQIIK
                     RGGQLGIKEIVLGMAHRGRLNVLANVMAKPFSAIFHEFKGGSSTPDEVEGSGDVKYHL
                     GSSS"
     gene            complement(1084..1962)
                     /gene="sucD"
                     /locus_tag="PKV23_05385"
     CDS             complement(1084..1962)
                     /gene="sucD"
                     /locus_tag="PKV23_05385"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009493481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HQX84903.1"
                     /translation="MSILINKKTKVICQGITGNNGTFHTEQAIAYGTQMVAGVTPGKG
                     GTKHIGLPVYDTVMEARERTGADASVIYVPPPFAADAILEAIDAEIPLVVCITEGIPV
                     QDMVKVKRALTGSSTRLIGPNCPGVLTPNECKIGIMPGNIFKKGSVGIVSRSGTLTYE
                     AVFQTTQEGLGQTTAVGIGGDPVKGTEFIDILELFLADPKTHSIIMIGEIGGSAEEDA
                     AEFIRKSKIKKPMAGFIAGRTAPPGRRMGHAGAIISGGKGGAESKIEAMKRAGIAVSD
                     SPAMLGKTLMKKLKRK"
     gene            complement(1962..>2929)
                     /gene="sucC"
                     /locus_tag="PKV23_05390"
     CDS             complement(1962..>2929)
                     /gene="sucC"
                     /locus_tag="PKV23_05390"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012110352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HQX84904.1"
                     /translation="VKTEAERMLGMTLVTHQTGPQGKLVQRLYIEEGTAIARELYLSC
                     LVDRETSTVAFIASTEGGVNIEEVAAKTPDKIATIHVDPASGYAPFVGRRIAAALKLE
                     GDQVKQCVKMVGQLYKAFTEKDMSLLEINPLVITKEGQLVCLDAKMNFDDNALDRHPD
                     IKAYRDLSEEDPKEIEASKYDLSYVALDGSIGCMVNGAGLAMATMDIIKLYGMEPANF
                     LDVGGGATKEKVTAAFKIITADPRVKGILVNIFGGIMKCDIIAEGVIAAVKEVGLQVP
                     LVVRLEGTNVDLGKKIIKDSALNVISADDLDDAAKKIVKAVKEAA"
BASE COUNT          566 a          813 c          870 g          680 t
ORIGIN      
        1 ggaggacgat cccagatggt attttacgtc gcctgaacct tccacttcat cgggagtgga
       61 ggagcccccc ttgaattcat ggaaaatggc ggagaagggc ttggccatca cattcgccag
      121 cacattcagg cggccgcggt gggccatgcc gaggacgatt tccttgatgc ccaattgccc
      181 gccgcgcttg atgatctgtt caagggccgg aatcatgctt tcgccgccat caaggccgaa
      241 acgcttggtg ccggtgtatt tcacgttgag aaaattctcg aagccttcgc cctcaatgag
      301 tttgcggaga atggccttct tgccttcgcg ggtgaaggaa atttccttgt cagcgccctc
      361 gatgcgctgc tgcagccagc ccttttgcaa gggatctgag atgtgcatga attcgacacc
      421 cagagtgtgg caataggtgc gtttcaggat atcggtgatc tggcggatgg tggcctgttc
      481 gaggcccagc accttgtcga ggaagatggg acggtcgaga tcggcttcgc tgaagccata
      541 ggtggagggg tccagttcgg gctgtgccgg acgttcggcc aatcccagcg gatcgagatc
      601 ggcggccagg tggccgcgca tgcggaaggc gcggatcatc atgagggcgc gcaccgaatc
      661 catggtggcc ttgcgcacct catctacgga aagcttggcg tcggcggcct tggacttggc
      721 ctcgatcttg gcgcccaccg ctttttccac ggcgggccag ttgccatcaa aggcggaaac
      781 gaggtcaccg gtcgcggcca gcggccaatc cttgcgggcc caggaaggcc cggaaacctg
      841 ctgcttggcg gaagcgggat catcatcaag accggcaaaa aactgccgcc agtggacatc
      901 cacggcggcg gggttttcca gatatttggc gtaaagcccc tcaatgaatt gggcattgcc
      961 gccatagagg aaggaagatt gttcaaaagc cgcaatcagg tctgtcttgt tcatcgccat
     1021 cacttccgaa gcccgggaac tctgtccgtg cctagagata tactacccaa agttttcttt
     1081 gtcttacttc cgtttcagtt tcttcattaa tgtcttgccg agcattgcag gcgaatccga
     1141 gacggcgatt ccggcccgtt tcatggcctc gatcttggat tcagcgccgc ccttgccgcc
     1201 tgagataatg gcgccggcat ggcccatgcg gcgccccggt ggtgcggtgc ggccggcaat
     1261 gaagcctgcc atgggctttt tgatctttga tttgcggatg aactctgcgg catcttcctc
     1321 ggcggaaccg ccgatttcgc cgatcatgat gatggagtgg gtcttgggat cagccaaaaa
     1381 caattccaaa atatcgataa actccgtacc tttcaccgga tccccgccta taccaacggc
     1441 tgtggtctgg ccaaggcctt cctgagtggt ctggaatact gcttcatagg taagcgttcc
     1501 ggagcgcgag acaataccca cggagccctt cttgaagata tttcccggca tgataccgat
     1561 cttgcactca tttggcgtga gcacgccggg gcaattcggg ccgatgaggc gggtgctgga
     1621 gcccgttaac gcgcgcttca ccttcaccat gtcctgcacc ggaatgcctt cggtgatgca
     1681 gacgacaagc ggaatttccg catcgatagc ttcgagaatg gcatccgccg caaagggcgg
     1741 cggcacatag atcacggaag catcggcgcc ggttctctcg cgggcttcca ttaccgtgtc
     1801 atagacggga agaccgatat gcttggtgcc acctttgccc ggcgtaacgc ccgccaccat
     1861 ctgggtgcca taggcaatgg cctgttccgt atggaaggtg ccgttgttgc cggtaatgcc
     1921 ctggcagatg accttggttt tcttattgat taaaatggac atcaggccgc ttcctttacc
     1981 gctttgacaa tcttcttggc ggcatcgtca aggtcgtcgg cagaaatgac attgagggct
     2041 gagtccttga tgatcttctt gccgagatcg acattggtgc cttcgaggcg caccactagg
     2101 ggaacctgca ggcccacttc ctttacggcg gcgatcacgc cttcggcgat gatgtcgcat
     2161 ttcatgatgc cgccgaaaat gttgacgagg atgcctttga cgcgcggatc ggcggtgatg
     2221 atcttgaacg cggcagtgac tttttccttt gtggccccgc ctcccacatc gaggaagttg
     2281 gcgggctcca tgccgtagag cttgatgatg tccatggtgg ccatggcgag gcctgcgcca
     2341 ttcaccatgc agccgatgga accgtccagt gccacatagg agaggtcata tttcgaggcc
     2401 tcgatttcct tggggtcttc ctcgctcaaa tcgcggtagg ccttgatgtc gggatggcgg
     2461 tcaagggcgt tgtcgtcgaa attcatcttg gcatcgagac agacaagctg gccttctttg
     2521 gtgatcacca gcggattgat ttcgagaagg ctcatgtcct tttcggtgaa ggccttgtag
     2581 agctggccca ccattttcac acattgcttg acctggtcgc cttcgagctt aagtgccgcg
     2641 gcaatccgcc tgcccacgaa gggcgcatag ccgctggcgg ggtccacatg gatggtggcg
     2701 atcttgtcgg gggtcttggc cgccacctct tcgatgttca cgccgccctc agtggaggcg
     2761 ataaaagcaa cggtggaggt ttcacggtcc acgagacagg agagataaag ctccctggcg
     2821 atggcggtgc cttcttcaat gtagaggcgc tggacgagtt tgccctgtgg cccggtctga
     2881 tgggtcacca gagtcatgcc cagcatacgt tcggcttctg tcttcacat
//
LOCUS       DAORVN010000219         2925 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_30732_length_2925_cov_3.645993, whole genome shotgun sequence.
ACCESSION   DAORVN010000219 DAORVN010000000
VERSION     DAORVN010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2925)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2925)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2925
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30732_length_2925_cov_3.645993"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /locus_tag="PKV23_05395"
     CDS             <1..351
                     /locus_tag="PKV23_05395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor sulfurase"
                     /protein_id="HQX84905.1"
                     /translation="ELAEIAGLMGIPEMKAEWLGANVVTSGIPDLTLLTPSTRLQFPS
                     GATIVVDMENFPCRQVADVVAQHHPEPKAGLVASAMHKRGVTAWVEREGIINGGDDII
                     VWLPPQRIYAHTGS"
     gene            348..944
                     /gene="mobA"
                     /locus_tag="PKV23_05400"
     CDS             348..944
                     /gene="mobA"
                     /locus_tag="PKV23_05400"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:TIGR02665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HQX84906.1"
                     /translation="MKIIGAIIAGGRSSRMGGREKAFLELASKPVILHVIEQFEPQVD
                     QLVINANGDAARFSEFGLEVVPDALSSLTTPLAGLHAALVFTKKVEAKMLVTVPSDTP
                     FLPFNLVAKLLEKALVRGAAIAASGDHEHYIIGAWKTDLLDDLDRAITSDNLIRVKDW
                     AQRAAAQSVIWPVEPYDPFFNVNAPEDLRIAEQILGAS"
     gene            941..1450
                     /gene="mobB"
                     /locus_tag="PKV23_05405"
     CDS             941..1450
                     /gene="mobB"
                     /locus_tag="PKV23_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018428278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HQX84907.1"
                     /translation="MTQKIIGVAGFKNAGKTTLVEKLVVDLTSRGFRISTVKHAHHSF
                     DIDHEGRDSFRHRKAGASEVAVVSSGRWAIIHELRSEAEPSLADILRKLEPCDLVIVE
                     GYKRDGHDKIEVRNLALDHPRLAGDDPTIVAIAATGGIADAPVPVFGRDDVRALSSFI
                     ISHMGLAKS"
     gene            1556..>2925
                     /locus_tag="PKV23_05410"
     CDS             1556..>2925
                     /locus_tag="PKV23_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006315386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQX84908.1"
                     /translation="MKTQARAVVIGGGVVGVSTLYHLAKKGWSDVVLVERKELTSGST
                     WHAAGLLPLFNLSYSVGQIHKYSVKLYGELEAETGQHVGFRKVSNIRVARTKDRWDEF
                     MYYAGIAETIGVKVNKLTPKQLKELWPLCDIDGVLGAIQHPDDGYIQPADLTQALAKG
                     ARSRGAEIYRNTTVTAIEQLPSGEWLVKTDKGDITCEHVVSCSGNFARKTGAMVGLDI
                     PVIPVEHQYIVTEPSPLIKERQAKGLPEMGVLREADSSWYLREENGGFVLGIYEKGAP
                     CCYVDGPSEQSEYELFQGELDRLEPYIETCITRVPAFGELGIKKIYNGAIAYTPDGSP
                     IIGPAWDRKNFWLNEGHSFGVTAAGGAGWQLAEWIVDGEPTIDMMGVDPRRFGPYATK
                     GYLKQKNEEAYANVFTMHYPEEERAAARPLKRTPCYDRMKDLGAVFGSVYGWERPGWF
                     APKGYAL"
BASE COUNT          610 a          800 c          894 g          621 t
ORIGIN      
        1 gagcttgcgg aaattgccgg tctcatgggc atccctgaaa tgaaagcgga atggctgggg
       61 gcaaatgtgg tgacatcggg aattcccgat ctgacgctgc tcacgccttc cacccggctg
      121 cagtttccat cgggcgccac gatcgtggtt gacatggaaa atttcccctg ccgtcaggtg
      181 gctgatgtgg tggcgcagca ccatcccgaa ccgaaggccg ggctggttgc ttccgccatg
      241 cataagcgcg gcgtcacggc ctgggttgaa cgagagggga tcatcaatgg cggtgacgac
      301 atcatcgtgt ggctgccgcc acagcgcatc tatgcccata cgggatcatg aagatcattg
      361 gagccatcat tgcgggcgga aggtccagcc gcatgggagg ccgcgagaag gcctttcttg
      421 aactggcttc caagcctgtg atcttgcatg taatcgaaca gtttgaaccc caggtcgacc
      481 agctcgtgat caatgccaat ggcgatgcgg caagattttc tgaatttggc ctggaggttg
      541 tgcctgatgc cttgtcgtca ctgacgactc cgcttgctgg cctgcatgcg gcgctagttt
      601 tcacgaagaa agtcgaggcg aagatgctgg tgacggttcc gtccgacacg ccattcctgc
      661 cattcaatct ggttgcaaag cttctggaga aggccttggt gaggggtgcg gccattgcgg
      721 cttccggtga ccatgaacac tacatcattg gcgcgtggaa aactgacctg ctggacgatc
      781 ttgatcgcgc cataacaagc gacaatctga tccgagtcaa ggactgggcc caaagggcag
      841 cggcgcagag cgtgatatgg ccggttgagc cttatgatcc ctttttcaat gtgaacgcgc
      901 cggaggattt acgcatcgcc gaacagatcc tgggtgcctc atgacgcaaa agataatcgg
      961 tgtcgccggc ttcaagaatg ccggcaagac cacgctggtt gaaaagctgg tggtcgatct
     1021 tacgagccgc gggtttcgca tttcgacggt aaaacacgcg catcattcct tcgacatcga
     1081 tcatgaaggc cgtgacagtt tccgccaccg caaggcggga gcctctgagg tagcggtcgt
     1141 ttcaagcggg cgctgggcca tcattcacga attgcgcagt gaagcggagc ccagccttgc
     1201 tgacatcctg cgaaagctgg aaccctgtga tctcgttatt gtcgaaggct acaagcgcga
     1261 cggccacgac aagattgaag tgcgcaatct ggcgcttgat cacccccggc ttgccggtga
     1321 cgatcccacc attgttgcca tcgcagcaac tggcggcatt gccgacgccc cggttccggt
     1381 ttttggccgg gatgacgtga gggcgctgag ttccttcatt atcagccaca tgggacttgc
     1441 gaagagctag tcccggtgtc ggattccgcc ctgcccgcgt cgctccactt cacgtctgtt
     1501 gaatttctct cactaatgtg gccacaatcg agtttccagg tccaaaggaa tttccatgaa
     1561 aacgcaggcg cgggcagttg ttatcggtgg cggcgtggtt ggcgtttcga cgctctatca
     1621 cctcgccaag aagggctgga gcgatgttgt cctggtggag cgcaaggagc tcacttccgg
     1681 gtccacatgg catgcggcag gactgcttcc gcttttcaac ctgtcctatt cggtgggcca
     1741 gattcacaag tattcggtga agctctatgg cgagcttgaa gcggaaaccg gccagcatgt
     1801 gggcttccgg aaagtgtcca acatccgcgt ggcgcgcacc aaggaccgct gggacgagtt
     1861 catgtattac gccggaatcg ccgagacgat cggcgtcaag gtcaacaagc tgacgccgaa
     1921 gcagttgaag gaactttggc cgctgtgcga cattgatggc gtccttggtg ccatccagca
     1981 tccggatgac ggctatatcc agcccgcgga cctcacccag gctttggcca agggggcgcg
     2041 cagccgaggc gcagagattt accgcaacac cacggtaacc gccattgagc agcttccttc
     2101 gggtgaatgg ctggtaaaga ccgacaaggg cgatattact tgcgagcacg ttgtttcatg
     2161 ttcaggcaat ttcgcacgca agacgggtgc catggtggga cttgatattc cggtcattcc
     2221 agtcgagcac caatatatcg tcaccgaacc ctcaccgctg atcaaggaac gccaagccaa
     2281 gggcctgccg gaaatgggcg tgctgcgcga agcggattca tcctggtatc tgcgcgaaga
     2341 aaacggcggt tttgttctcg gcatttatga gaagggtgcg ccgtgttgct atgtggacgg
     2401 gccttccgaa caaagcgaat atgaattgtt ccagggcgag ctcgatcgcc tggagcccta
     2461 tattgaaacc tgcattacgc gggttccggc cttcggcgaa ctgggcatca agaaaatcta
     2521 caatggcgcc attgcctaca cgccggacgg ctcgccgatc atcggaccag catgggaccg
     2581 gaagaatttc tggctgaacg agggccattc ctttggcgtc acggcggccg gcggtgcggg
     2641 ttggcaattg gcggaatgga tcgtcgatgg cgaacccacc atcgacatga tgggcgtcga
     2701 tccgcggcgc ttcggcccct atgccacgaa gggctatctc aagcagaaga acgaagaggc
     2761 ttacgccaac gtctttacca tgcattatcc ggaagaggaa cgtgcggcgg cccgtccctt
     2821 gaagcgtacg ccgtgctatg accggatgaa ggatctgggc gcggtgttcg gttcggtcta
     2881 cggctgggag cggccgggct ggtttgcgcc gaagggctat gcact
//
LOCUS       DAORVN010000220         2920 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_30844_length_2920_cov_2.753578, whole genome shotgun sequence.
ACCESSION   DAORVN010000220 DAORVN010000000
VERSION     DAORVN010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2920
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30844_length_2920_cov_2.753578"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1197
                     /locus_tag="PKV23_05415"
     CDS             <1..1197
                     /locus_tag="PKV23_05415"
                     /inference="COORDINATES: protein motif:HMM:NF037421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX84909.1"
                     /translation="RFTEQVEFVDAGTKLTFGHEFGDPAIRASLQLAAQKWVVGPLNI
                     GGTEAFKRANALEAVRLGIDSRLAGIEFGVLEAARTQVAAEHEAMKTAVAKGAQRIQE
                     ATNAERLIVMAEKNRHATRLLGDAEGSQKDRLPVLGMAQAQEQGEAVRVLPVVKDQIH
                     FDLDLETAGLAEFKKNGAKPAFVQAGAKPGQFVAVVGIAGNRHNPAVMQQVDSFKSDL
                     LKRYGPQGETLVAHGKAPFEHQAGGSKSYFVRTRNADGVEREQWGVDLERALQEAAAR
                     PGDLITLRRAGQQEVKVNAPVKGPDGKPTGETQEKVVKRNGWEVKTIQKADELRHNAP
                     DATKQEATKPGEVIETGNAEPCKHTQAEFEKHSGMSKAKSLEKPVGKEIEPQNKAPKR
                     STGPGF"
     gene            complement(1616..2791)
                     /locus_tag="PKV23_05420"
     CDS             complement(1616..2791)
                     /locus_tag="PKV23_05420"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQX84910.1"
                     /translation="MNAMKHTEGREISTMGYCRHYPTLLLVPESVSLAIRQEKETEEE
                     ALNCQKLDKEALLLIQEKAEAEAPDTINQYVPHTVPVFQEAACLKALEAIKHEERSPK
                     IAMTLEQALKHGGFRPLLTRKIDDTLVDFDALEVEMPNFSEALDMLRGELLIATRQPP
                     EEFRIEPLLLDGPPGIGKTRFARAVARILNVGFSNVSLGTVTGGFELAGLSQGWGTSR
                     PGRIASVLADTPLSACPVLLLDEIDKINGDFRHDPTPVLLDLLERDSARRFRDECLGV
                     EIDASRMIIFATSNDKSLLSDPLLTRLRVVDCKEPSLEERRGMAERIVANYATTTDIY
                     FAADALTQIVVDATNMREIARRLREEIGRALRRDEGNVVYPRHVRDNEKPRQAMGFV"
BASE COUNT          705 a          755 c          852 g          608 t
ORIGIN      
        1 cgattcaccg agcaagtcga gtttgttgat gcaggaacaa aactgacctt tgggcatgaa
       61 tttggcgacc cggcaattcg ggcaagcctg caattggccg cgcagaaatg ggtagtaggg
      121 ccgctgaata ttggtggcac tgaagcgttc aagcgggcaa acgccttgga agctgttcga
      181 ctgggaatcg atagccgact ggccggcatc gagtttggcg tgcttgaagc ggcacggact
      241 caggtggccg ccgagcacga ggcgatgaaa acggcagtgg ccaagggtgc acagcgcatc
      301 caggaagcga ctaatgccga gcggttgatt gtgatggcag agaagaatcg gcacgcgacc
      361 cgcctgctgg gtgatgcgga ggggagccag aaagatcgct tgccggtgct gggtatggcg
      421 caggcgcaag agcagggcga ggcggtgcgg gtgctgccgg tcgtcaagga tcaaattcac
      481 ttcgatctgg atctcgaaac cgctggattg gctgaattca aaaagaacgg cgccaagccg
      541 gcgtttgtgc aggccggggc gaaaccgggg cagttcgttg ccgtggtcgg gattgccggc
      601 aatcgccaca atccggcggt gatgcagcaa gtcgattcgt tcaaaagcga ccttttgaag
      661 cgttacgggc cgcagggcga gacgcttgtg gcgcacggca aggccccgtt cgagcatcaa
      721 gctggcgggt ccaaaagcta cttcgttcgg acgcgaaatg ctgatggtgt tgaacgcgag
      781 cagtggggcg tcgaccttga gcgggcgctg caagaagcgg cggcgaggcc gggtgaccta
      841 attacattac ggagggctgg ccagcaagag gtcaaggtca atgcgccggt caaaggtccg
      901 gacggcaaac ctacgggcga gacacaagaa aaggttgtta agcgaaatgg ttgggaagtt
      961 aaaaccattc aaaaagctga tgaattgcga cataacgctc cggatgcgac aaaacaagaa
     1021 gccacaaagc ccggagaggt tatagaaacc ggcaatgcag agccgtgcaa acacacgcag
     1081 gcggagtttg aaaagcactc cggaatgagc aaggctaaat cccttgaaaa gcctgtgggt
     1141 aaagagattg agccacaaaa taaagcccca aaaaggtcaa cagggccggg tttttgagcg
     1201 ccagatggat tacttgaggg tcttgaacct cagccatgag cttctgtcca tattccccga
     1261 gttatccacc gccggccggc cggcagacag cctgctatag aaggagcgca gtctgccggc
     1321 cgaccgtgga taactctcca tggtgcgaca aaaactttac cttcatgaca tcaagagagt
     1381 aaagcccgaa aaccctgcgt cgctatgctt cgactgtcca gtttgctctg gaatgcgcac
     1441 aagtatcact gaaaccttca ccaagacaaa tctaatagca aaataagcca gctcgccacg
     1501 tgtcttggtg aaggtttcgg tgaacctatg ttccgattgg gcatgcccca tcacgagggc
     1561 gacaaatgca gcctttcaat tggtgcccac ttagccgata gtttggcaac tttgatcata
     1621 caaaccccat tgcctgccgg ggcttttcgt tatctcgcac atgccgggga taaacaacat
     1681 tcccctcatc gcgccgcagg gcgcgcccga tctcttcgcg caaccggcgt gcaatttcgc
     1741 gcatgttcgt ggcatcgacc acgatctggg taagggcgtc cgccgcaaaa taaatgtcag
     1801 tcgtggttgc gtagttggct acgatgcgtt cagccatgcc gcgccgctct tcgaggcttg
     1861 gctctttgca atcaaccacg cgcagccgag ttaatagcgg atcactgagc aggcttttat
     1921 cgttgctggt cgcaaagatg atcatgcggg acgcgtcgat ctcaacgccc aggcattcat
     1981 cacgaaaacg ccgggcactg tcccgttcga gaaggtcaag caggacgggc gtcggatcgt
     2041 gccggaagtc gccgttgatc ttgtcaattt catcgagtaa tagcacgggg caagcgctca
     2101 acggcgtatc ggcaagcact gatgcaattc gtccagggcg tgatgtgccc cacccttgcg
     2161 agagcccagc caactcaaat ccacccgtaa cggtgccgag cgagacgttg gaaaacccca
     2221 cgttcagaat gcgcgcaacc gcgcgggcaa atctggtttt gccaatgcct ggcgggccat
     2281 ccagaagcag aggctcaatg cggaattctt cgggcggctg gcgggtagca atcagcaatt
     2341 caccacgcag catgtcgagc gcctcggaga agttcggcat ctcaacttcg agggcatcaa
     2401 agtctacaag cgtatcgtcg attttacgtg tcagaagtgg gcggaaaccg ccgtgtttaa
     2461 gcgcctgctc aagcgtcatc gcaatctttg ggctcctttc ctcatgcttg atggcctcaa
     2521 gggctttcaa acatgccgcc tcttgaaata ccggtaccgt atggggtacg tattgattga
     2581 tggtgtccgg cgcttctgct tccgcttttt cctgaatgag aaggagggct tctttgtcga
     2641 gcttctggca attcaaggct tcttcctcag tttctttctc ctgccgaatt gccagcgaca
     2701 cagactcagg gacgagcagc agggtcgggt agtggcggca gtaacccatc gtcgagattt
     2761 cacggccttc agtgtgtttc atcgcgttca taataatttc cttgtttatt actgtaatat
     2821 ggtgtaagat catcttacga tgtaatacat aattggagat taaaccatgg caatcgcaaa
     2881 agcaacttat gaaacagttc gggatgcaat cgacagactc
//
LOCUS       DAORVN010000221         2911 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_31026_length_2911_cov_2.396008, whole genome shotgun sequence.
ACCESSION   DAORVN010000221 DAORVN010000000
VERSION     DAORVN010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2911
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31026_length_2911_cov_2.396008"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..452)
                     /locus_tag="PKV23_05425"
     CDS             complement(<1..452)
                     /locus_tag="PKV23_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019862479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HQX84911.1"
                     /translation="MEIGMYTFADMGPAISAGERLNNLLEEIDLADKVGLDVYGVGEH
                     HRPDYAASAPVIVLAAAAARTKTIRLTSAVTVLSSDDPVRVYQNFATLDLLSHGRAEV
                     MVGRGSFIESYPLFGYDLNDYDVLFSEKLDLLLKLRAQEHVTWSGTIRA"
     gene            complement(515..2467)
                     /gene="thrS"
                     /locus_tag="PKV23_05430"
     CDS             complement(515..2467)
                     /gene="thrS"
                     /locus_tag="PKV23_05430"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HQX84912.1"
                     /translation="MITLTFPDGAKREIEQGLSAAQVAAGISKSLEKKAVAAVVNGML
                     VDLNDPITADATLQIVTRDDARALELIRHDCAHVLAEAVQELYPGTQVTIGPVIENGF
                     FYDFFRNEPFTPEDFAPIEKKMREIIQRDKPFTKSVKTREEAKEFFRNKGEMFKVELV
                     DAIPGNEDIKFYDQGGWIDLCRGPHMTSTGKIGNAFKLMKVAGAYWRGDSKNPMLTRI
                     YGTAWASDKDLQAYVTALEEAEKRDHRKLGREMDLFHFQEEGPGVVFWHAKGWTIFQQ
                     LIAYMRRRLSKLDYQEVNAPQVLDKSLWETSGHWGWYRETMFAVTSAGEETGDEQVFA
                     LKPMNCPGHVQLFKHGLRSYRELPMRLAEFGNVHRYEPSGALHGLMRVRGFTQDDAHI
                     FCTPEQMADECLKINDLILSTYKDFGFEEIVVKLSTRPEKRVGTDADWDRAEDIMQKV
                     LLQIAEASGGKIKTAINPGEGAFYGPKFEYVLRDAIGRDWQCGTTQVDFNLPERFGAF
                     YIGANGEKHVPVMIHRAICGSMERFLGILIEHHAGHFPLWLAPVQVMVTTITSEGDEY
                     AIKVRDDLRKAGLRADADLRNEKINYKVREHSLAKVPVILVVGKREMDEQAVNMRRLG
                     SQDQKSMSLADAIAALVSEATAPDLR"
     gene            2680..>2911
                     /locus_tag="PKV23_05435"
     CDS             2680..>2911
                     /locus_tag="PKV23_05435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX84913.1"
                     /translation="MNRFLLGLAVAIMLPASSAFAADLDVPPPDNLRPATFDWSGPYL
                     GIFGAAIAVDGTYDATCTAAVPCAVPTYTDIEH"
BASE COUNT          562 a          933 c          771 g          645 t
ORIGIN      
        1 gcacggattg tgcctgacca cgtcacatgc tcctgcgccc gcagcttcaa gagcaggtcc
       61 agcttctcgg aaaacagcac gtcataatcg tttaggtcat agccaaacag cggataggat
      121 tcgatgaacg acccccgccc caccatgacc tcggcccggc catgggacag cagatcaagc
      181 gtggcaaaat tctggtacac ccgcactggg tcgtcggaag aaagaaccgt caccgctgaa
      241 gtcagccgga ttgtcttggt ccgcgccgcg gccgccgcca gcacgataac gggggccgag
      301 gctgcatagt ccggccggtg gtgctcgccc accccataga catcaagccc gaccttgtcg
      361 gccagatcga tttcctcaag caggttgtta agccgctctc ccgccgagat tgccgggccc
      421 atgtcggcga aggtgtacat ccctatttcc atgggagaca tatgtcgcgg ttatgatgac
      481 cgttcaagta tcaggcttgt aaccgaaact ggagctatct taaatccggc gcagtcgcct
      541 cgctcaccag cgcagcaatc gcatctgcca gactcatcga cttctggtcc tgactcccga
      601 gcctgcgcat gttcaccgcc tgctcgtcca tctcgcgttt gccgaccacc aggatgaccg
      661 gcacctttgc cagggaatgt tcgcgcacct tgtagttgat cttctcgttg cgcaggtcgg
      721 cgtcggcgcg gagacccgcc ttgcgcaaat catcgcgaac cttgatagca tattcatcgc
      781 cctcggaggt gatcgtcgtc accatcacct gcaccggcgc cagccacagc gggaaatgcc
      841 ccgcatggtg ttctatgaga atgcccagaa accgttccat ggagccgcag atggcccggt
      901 ggatcatcac cggcacatgc ttctcgccgt tggcgccgat gtagaatgcg ccgaaccgct
      961 ccggcaggtt gaagtccacc tgcgtggtgc cgcattgcca gtcgcggccg atggcgtcgc
     1021 gcagcacata ttcaaacttg gggccgtaga aggctccctc gcccggatta attgccgtct
     1081 taatttttcc tcccgaggct tctgcgatct gcagcagcac cttctgcatg atgtcttcgg
     1141 cacggtccca gtcggcatct gtccccacgc gcttttccgg ccgcgtcgaa agcttcacca
     1201 cgatttcctc gaaaccaaag tccttatacg ttgaaaggat caaatcgttg atcttgaggc
     1261 attcatccgc catctgctcc ggcgtgcaga aaatatgggc gtcatcctgc gtgaaccccc
     1321 gcacccgcat caggccatgc agcgcacccg atggttcgta gcggtgcacg ttgccgaatt
     1381 ccgccagccg catcggcaac tcgcgatagc tccgcaaacc gtgtttgaat aactgcacgt
     1441 gcccagggca gttcatcggc ttgagcgcaa atacctgctc gtcgcctgtt tcctcaccgg
     1501 cagaagtgac ggcaaacatg gtttcacggt accagcccca atgcccggac gtttcccaaa
     1561 ggctcttgtc caggacctgc ggcgcgttca cctcctggta gtcgagcttt gacagccgcc
     1621 ggcgcatata ggcaatgagt tgctggaaaa tcgtccagcc cttggcgtgc cagaacacca
     1681 cgccgggtcc ctcttcctgg aagtggaaca ggtccatttc gcggcccagc ttgcggtggt
     1741 cgcgcttctc ggcttcttcc agagccgtca cataggcctg caaatccttg tcgctggccc
     1801 aggccgtgcc gtagatccgc gtcagcatcg ggttcttcga atcaccccgc caataggctc
     1861 cggccacctt catcagtttg aaggcattgc cgatcttgcc cgtcgacgtc atgtgcgggc
     1921 cgcggcacag gtctatccag ccgccctggt catagaactt gatgtcctcg ttgccaggaa
     1981 tcgcatcgac cagctcaacc ttgaacatct cgcccttgtt gcggaagaac tcctttgcct
     2041 cctcgcgcgt cttcaccgat ttcgtgaacg gcttgtcacg ctggatgatc tcgcgcattt
     2101 tcttttcgat gggcgcgaag tcctccggcg tgaacggctc attgcggaaa aagtcataga
     2161 agaagccgtt ctcgatcacc gggccgatgg taacctgcgt tcccgggtaa agctcctgca
     2221 ccgcttccgc cagcacatgg gcgcagtcat ggcggatgag ttcaagcgcc cgcgcgtcat
     2281 cgcgcgtgac aatctgcagc gtggcatcgg ctgtgatcgg atcattgaga tcgaccagca
     2341 tgccattgac cactgcggcc acggcctttt tctcgagtga ttttgaaatg ccggccgcga
     2401 cctgtgcggc actcagtccc tgttcgattt cgcgcttggc gccgtcaggg aaagtcaatg
     2461 taatcatgct gtcctcctgc tcactcctgc caacacatgc aggtaagcgt ttgatgggtt
     2521 ttgggtgccg cgcttatggc cttaaacgct gccttcggtc aagcctgccc cgaaaagcct
     2581 gcatattaac cagctcttag tttttccata accgcgtatt aaccttaaca cttcatgttc
     2641 tgcccgaacg acagcgtaac aaatgggttg ggtaacgaca tgaatagatt tcttctcggt
     2701 ctcgcggttg caatcatgct tccggcatca tcggcattcg ccgctgatct cgatgtaccg
     2761 ccgccagaca atctgcggcc ggcaaccttt gactggtcgg gaccgtatct cggcatcttc
     2821 ggcgcggcga ttgccgttga cggcacctac gatgccactt gtaccgcggc tgtgccctgc
     2881 gccgtgccca cctataccga catcgagcac a
//
LOCUS       DAORVN010000222         2910 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_31041_length_2910_cov_2.697373, whole genome shotgun sequence.
ACCESSION   DAORVN010000222 DAORVN010000000
VERSION     DAORVN010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2910)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2910)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2910
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31041_length_2910_cov_2.697373"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2097)
                     /gene="pheT"
                     /locus_tag="PKV23_05440"
     CDS             complement(<1..2097)
                     /gene="pheT"
                     /locus_tag="PKV23_05440"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HQX84914.1"
                     /translation="MKFTFSWLKDYLDTTASLEQIVDGLVGVGLEVEEVSDKTKLLAP
                     FKVAYVIEAVKHPNADKLRLCKVETDTGIYQVVCGAPNARSGMKGIMALPGAFIPGTG
                     ITLEKGVIRGQESQGMLCSERELQISLEHDGIIDLKGDWPIGTSAVKALGLDDPMIYV
                     KVTANRPDALGIYGIARDLAAKGLGKLKPLNAKPVAGTFDSPIKVSLDFEDTKPCPLF
                     VGRYFKGVKNGPSPEWLQRRLKAIGLRPISTLVDITNYITFAYGRPLHVFDADKVKGN
                     IRARMARDGEELAALDGKTYALTAEMTVIADDSSAEGIAGIIGGEPSGCSETTTNVFL
                     EAAYFDPLRTAATGRKLGVISDARYRFERGVDPAFTQVGAEIATQMILELCGGAASHL
                     VIAGKAPLKPRTYTLRATRVKTLAGLDLPLAEQRRILLDLGFGVKETPAGLECAPPSW
                     RPDVHDETDLVEEVCRIHGLENIPFTAMERSHPIATPVLNPLQRRMLAARRGLAARGF
                     NEAVTWSFIPEAHAKLFGGGQAELKLANAISSELTDMRPSLLPNLIAAAGRNMKRGFA
                     DLSLSEVGHAYAGDRPEDETLRAAGIRRGNAVTRNWQGGARAVDAFDAKADALAVLEA
                     AGAATASLQVVAGAPAWYHPGRSGAIQMGPQNKLAYFGEIHPRVLAAMDVKGPLVAFE
                     VVLNAVPVPKAKNATRA"
     gene            complement(2094..>2910)
                     /gene="pheS"
                     /locus_tag="PKV23_05445"
     CDS             complement(2094..>2910)
                     /gene="pheS"
                     /locus_tag="PKV23_05445"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006934061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HQX84915.1"
                     /translation="LASEKIDVTLPSRAEPRGTVHPVSQVWEEVVQIFGDLGFAVAEG
                     PHIEDDFHNFTALNIPPEHPARQEHDTFYFSEKPDGSRMVLRTHTSPVQIRTMLTQKP
                     PIRIIAPGRTFRNDSDMTHTPMFNQIEGLVIDETTHMGHLKWILAEFCKSFFEVDDVK
                     MRFRASHFPFTEPSMEVDINCSWEGGQVKIGQGSSWLEILGSGMVHPNVIRAGGLDPD
                     KYQGYAFGMGLDRIAMLKYGIPDLRAFFEADLRWLPHYGFKALNVPTLPGGLS"
BASE COUNT          562 a          948 c          843 g          557 t
ORIGIN      
        1 ggcgcgcgtg gcgtttttgg ctttcggcac cggaacggca tttagcacca cttcgaaagc
       61 caccagcggc cccttcacgt ccatggccgc caggacccgc ggatgtattt cgccgaaata
      121 cgccagcttg ttctgcggcc ccatctggat ggcgccggaa cggccgggat gataccatgc
      181 gggcgctccc gccaccactt gcaggctcgc tgtggcggcc cctgcggcct caagcaccgc
      241 aagagcgtca gccttggcat caaaggcatc aactgcccgt gcgccaccct gccagttgcg
      301 cgtgacagca ttcccgcgcc gaatgcccgc cgcccgcaag gtctcgtcct ccggccggtc
      361 gcccgcatag gcatggccca cctcgctcaa gctgagatcg gcaaagccgc gcttcatgtt
      421 gcgccctgcc gccgcaatga gattgggaag cagcgagggc cgcatatccg tgagttccga
      481 cgaaatggca ttggcaagct tcagctccgc ctgtccgccg ccgaatagtt tcgcatgcgc
      541 ctccggaatg aacgaccagg tcaccgcctc attgaagccc cgcgccgcca gcccgcgccg
      601 cgcagcgagc atgcgcctct gcaggggatt gagaaccggc gtggcgatgg gatgggagcg
      661 ttccatagcc gtgaaaggaa tattctcgag gccatggatg cggcagacct cttccaccag
      721 gtcggtttcg tcgtgaacgt cgggccgcca ggagggtggc gcacattcta ggcctgccgg
      781 tgtttccttc acgccgaagc caaggtccag gagaatgcgc ctctgttcgg caagcggcag
      841 atcgagtccg gccagggttt tcacccgtgt cgcccgcaag gtataggtcc gcggcttcag
      901 cggggccttg cccgcaatca ccagatgcga ggccgcgcca ccgcaaagct caaggatcat
      961 ctgtgtggca atctccgccc ccacctgcgt gaaggccggg tcaacgccgc gctcgaagcg
     1021 gtagcgcgca tcggaaatca cgccgagttt ccgccccgtg gcagcggtgc gcaggggatc
     1081 gaaataggcc gcttccagga atacatttgt cgttgtctcc gagcagcccg aaggctcgcc
     1141 gccgataatc ccggcaatgc cctcggcaga actgtcatcg gcgatcaccg tcatctctgc
     1201 tgtgagcgca taggtcttgc catctagggc ggccaattct tcgccatccc gcgccatgcg
     1261 cgcccggata ttgcccttca ccttgtccgc gtcgaacaca tgcagcggcc ggccataagc
     1321 aaaggtgatg tagttggtga tatcgaccaa agtcgaaatc gggcgcagcc caatggcctt
     1381 cagtcgccgc tgcagccatt ccggtgacgg gccattcttc acgcccttga aatagcgccc
     1441 gacaaaaagc gggcagggtt tggtgtcttc gaaatccagc gacaccttga tcggtgaatc
     1501 gaacgtgccc gcaacgggct tggcattcag cggcttcagc ttgcccaagc ctttcgccgc
     1561 gagatcgcgc gcaatgccgt aaatccccag cgcatcaggc cggtttgccg tcaccttcac
     1621 atagatcatc gggtcatcaa ggccgagcgc cttcaccgcc gacgtgccaa tgggccagtc
     1681 gcccttgaga tcgatgatgc cgtcatgctc caggctgatc tgcagctcgc gctccgagca
     1741 cagcatgccc tggctctcct ggccacggat cacgcccttt tccaacgtaa tcccggtgcc
     1801 cggaatgaac gcacccggca atgccatgat gcccttcatg ccactccgtg cattgggcgc
     1861 gccgcagacc acttgataga tgcccgtgtc cgtctcgacc ttgcacagcc gcagcttgtc
     1921 cgcattggga tgcttcaccg cctcgatgac ataggccacc ttgaacggcg ccagcagctt
     1981 cgtcttgtcg gaaacttcct cgacctcaag gccaacgcct acaagcccgt ccacgatctg
     2041 ctcaagcgaa gccgtcgtgt cgagataatc cttcagccag gaaaacgtga atttcatgac
     2101 aggcctcccg gcagcgtcgg cacgttgagc gccttgaatc cgtagtgcgg gagccagcgc
     2161 aaatcggcct cgaagaaggc acgcaggtcg ggaatgccgt atttcagcat ggcgatgcgg
     2221 tcgagcccca tgccgaaggc atagccctgg tacttatccg gatcgaggcc gcccgccctg
     2281 atcacattgg gatgcaccat gccgctgccg aggatttcca gccatgacga accctggccg
     2341 attttcacct ggccgccttc ccaggagcag ttgatgtcca cttccatgga aggctccgtg
     2401 aaagggaaat gcgaggcgcg gaagcgcatc ttcacatcgt cgacttcaaa gaagctcttg
     2461 cagaattccg cgagaatcca tttgagatgg cccatatggg tcgtctcgtc gatcaccagg
     2521 ccttcgatct ggttgaacat cggcgtatgg gtcatgtcgc tgtcgttgcg gaaggtgcgc
     2581 cccggcgcga tgatgcggat gggcggcttc tgcgtcagca tggtgcggat ctgcaccggg
     2641 ctcgtatgcg tgcgcaaaac catgcggctg ccatccggct tttcgctgaa ataaaacgta
     2701 tcgtgctcct gccgcgccgg atgttcgggg ggaatattga gcgccgtgaa attgtgaaaa
     2761 tcatcttcga tatgcgggcc ttccgccacc gcaaatccga gatcgccgaa aatctgcacc
     2821 acctcttccc acacctggga gacgggatgc accgtgccgc gcggctcagc gcgagaaggc
     2881 agggtcacat caattttttc agatgccaaa
//
LOCUS       DAORVN010000223         2907 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_31081_length_2907_cov_2.800842, whole genome shotgun sequence.
ACCESSION   DAORVN010000223 DAORVN010000000
VERSION     DAORVN010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2907
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31081_length_2907_cov_2.800842"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1253)
                     /locus_tag="PKV23_05450"
     CDS             complement(<1..1253)
                     /locus_tag="PKV23_05450"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HQX84916.1"
                     /translation="MTAFRRLEKGGRIDRSKPVSFTYDGKPLSGFAGDTLASALLANG
                     KTLTSRSFKYHRPRGIISAGVEEPNGLFTLGKNEPNIPATTVDLKDGLVVHSQNAWPS
                     PDFDVMAVNSLLSPLFQSGFYYKTFMGPLKKSWMFYEPFIRRAAGLGKGSYEKDSDRY
                     DTAHEFCDVLVVGSGPAGLAAAATAGRSGARVVLVEQDSEFGGQLLSERTAELEAWRK
                     AQLTELASLSNVKLRARTTAQGLYDDNFVVLIQRHEEFDATKGKPRQTLISLRAKSII
                     FATGALERPLVFSNNDRPGVKLASAVRTYLNRFAVAPLKRVCVVTNNDTAYLTAFDLA
                     EAGIAVTMADHRKVIPSNLLSRAAMLGMAMFPGTGVADVEGAKQVSRVQLAGAHSASI
                     ECDVLAMSGGWSPAVHLTSHGGIKP"
     gene            complement(1250..1492)
                     /locus_tag="PKV23_05455"
     CDS             complement(1250..1492)
                     /locus_tag="PKV23_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011440041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit delta"
                     /protein_id="HQX84917.1"
                     /translation="MRIPCPHCGERDVSEFRYGGDATKERPKHGEGDLKAWHDYYFLF
                     DNPKGPHTEYWQHVLGCRHWFKLTRNTATNEIGEAS"
     gene            complement(1489..2748)
                     /locus_tag="PKV23_05460"
     CDS             complement(1489..2748)
                     /locus_tag="PKV23_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006330125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008115 - sarcosine oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0046653 - tetrahydrofolate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit beta family protein"
                     /protein_id="HQX84918.1"
                     /translation="MFKPYSALALAQHAFSPERPWPRAWRAPEPKSSYDAIIIGGGGH
                     GLATAYYLAKNHGMRNVAVLERAYIGSGNVGRNTTLIRSNYVIDGNTQFFEHSMELWE
                     GLSHELNFNCMVSHRGQVVLATSPVQLDTFARRGNIMRLNGIDAELLDRGQVMALLPY
                     LDYSREARWPIWGGILQERAGTVRHDAVAWGYARAADTLGVDIIENCEVTGFVMDEGK
                     AVGVETSRGRILGGKIGIAVAGHSSHVAKMAGLRLPIESHILQAFVTEPVKPMVHHVV
                     SWGAELFYLSQSDKGGMVFGGHIDGFNTYTQRGQFPRIQTVAECAVSLLPFMSKLRLL
                     RHWGGIMDMTPDGSPFICRTDIRNLYFNGGWCYQGFKATPASGWTFAHTIAHDEEHEL
                     NRCYALDRFEKGRELDDYGIGNWTYKQ"
BASE COUNT          555 a          831 c          961 g          560 t
ORIGIN      
        1 attggcttga tgccgccgtg cgaggtgaga tgcaccgccg ggctccagcc gccggacatg
       61 gcgagcacat cgcattcgat ggaagcggaa tgggcgcctg caagctgcac gcggcttacc
      121 tgcttggctc cttccacatc ggcaacgccg gttccgggga acatggccat gccgagcatt
      181 gcggccctgg agagcaaatt ggagggaatg accttgcgat ggtcagccat ggtgacggcg
      241 atgcctgctt cggcaaggtc gaaggcggtg aggtaagccg tgtcgttgtt ggtgacgaca
      301 cacacgcgct tgagcggggc cacggcgaag cggttgagat aggtgcgcac ggcggaggcc
      361 agcttgacgc cggggcggtc attattggaa aacaccaaag gccgttccag cgcgccagtg
      421 gcaaagataa ttgatttcgc gcgcagtgag atcagcgtct ggcgcggctt gccctttgtg
      481 gcatcgaact cttcgtgacg ctggatcagc acgacgaaat tatcatcata gaggccttga
      541 gcggttgtcc gtgcgcggag tttcacgttt gaaagggacg ccaattcagt gagttgggct
      601 ttgcgccagg cttccagttc ggccgtccgt tcagagagca attggccgcc gaattcggag
      661 tcctgctcaa ccagcacaac gcgcgcgcct gaacgccctg cggtcgctgc agcggcaagg
      721 ccggcggggc cggagcccac cacaagcaca tcgcagaatt catgggccgt atcgtagcga
      781 tccgaatcct tttcgtagga gcctttgcca agacccgcgg cgcggcggat gaagggttcg
      841 tagaacatcc aggacttctt gagcggcccc atgaaggtct tgtagtagaa gccggactgg
      901 aacagcgggc tgaggagaga atttaccgcc atcacgtcaa agtcaggtga cggccaggca
      961 ttctgcgaat ggacgacgag gccatctttg agatcaacgg tcgttgccgg aatgttgggc
     1021 tcgtttttgc caagggtgaa gaggccgttg ggttcttcga cgccggcgga gatgatgccg
     1081 cgcggccggt ggtatttgaa actgcgcgag gtgagggtct tgccattggc gagcagcgcc
     1141 gaggccagtg tgtcgccggc gaatccgctg agtggcttac cgtcataggt gaagctgacc
     1201 ggcttgctgc ggtcgatgcg gccgcccttc tcaagcctgc ggaaggcggt catgacgcct
     1261 ctccaatttc gttggtggcg gtgttgcgcg tgagcttgaa ccagtggcgg cagcccagca
     1321 cgtgctgcca gtattcagtg tgcgggccct tgggattatc gaaaaggaag taataatcgt
     1381 gccaggcctt gagatcgccc tcgccgtgtt tggggcgctc tttcgtggca tcgccgccgt
     1441 agcggaattc gctcacgtct ctttcgccgc aatggggaca cgggattctc attgtttgta
     1501 agtccagttg ccgatgccgt agtcgtcgag ctccctgccc ttttcgaagc ggtcgagggc
     1561 gtagcagcgg ttgagctcat gctcctcgtc atgggcgatg gtgtgggcaa aggtccagcc
     1621 cgatgcgggt gtggccttga agccctggta gcaccagccg ccattgaaat agaggttcct
     1681 gatgtcggtg cggcagatga aggggctgcc gtcaggcgtc atgtccatga tgccacccca
     1741 gtgacgcaaa aggcggagct tcgacatgaa ggggagcagc gacacggcgc attccgccac
     1801 ggtctggatg cgggggaact ggccgcgctg ggtgtaggta ttgaagccgt cgatatggcc
     1861 gccgaagacc atgccgccct tgtcggactg cgagagatag aacagctcgg caccccagga
     1921 cacgacatga tgcaccatgg gcttcaccgg ctcggtgacg aaggcctgga ggatgtggct
     1981 ctcaatgggc agccggaggc ccgccatttt cgccacatgg gatgagtgcc cggccaccgc
     2041 gatgccgatc tttccgccga gaattcgccc ccgcgaggtt tcgacgccga ccgccttgcc
     2101 ttcatccatc acaaatccgg tcacttcgca attctcgatg atgtcgacgc ccaaggtatc
     2161 ggcggcgcgg gcatagcccc aggccacggc gtcatggcgc acggtgcctg ccctctcctg
     2221 caggatgcca ccccagatgg gccagcgggc ttctctggaa taatcgaggt agggcagcag
     2281 cgccatgacc tggccgcggt cgagcagttc cgcgtcgatg ccgttgaggc gcatgatatt
     2341 gccgcgccgg gcaaaggtat cgagctgcac cgggctggtg gcgagcacga cctggccgcg
     2401 gtgcgagacc atgcagttga aattcagctc atgggagagg ccttcccaga gctccatgga
     2461 atgctcgaaa aactgggtgt tgccgtcgat cacataattg gagcggatga gcgtggtgtt
     2521 gcggccgaca ttgccggagc cgatataggc gcgctcgagc acggccacat tgcgcatccc
     2581 atggtttttg gcgaggtaat aggcggtggc caggccatgc ccgccgccgc caatgatgat
     2641 ggcgtcgtag gaactcttgg gctcaggcgc gcgccaggcc ctcggccacg gccgttcggg
     2701 cgaaaaggca tgctgggcaa gggccagggc tgagtacggt ttgaacacga cagaccccct
     2761 accagaattt cccatcaacc gacaagacga ggatttttca cctccgccgc ttgagccatt
     2821 ccacgccctc gctcaccagc atggccgcca ccaacatggc gaggaaggca taggccaggg
     2881 gcatgaccgt gccgtcatac tgctggc
//
LOCUS       DAORVN010000224         2900 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_31220_length_2900_cov_2.746573, whole genome shotgun sequence.
ACCESSION   DAORVN010000224 DAORVN010000000
VERSION     DAORVN010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2900)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2900)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2900
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31220_length_2900_cov_2.746573"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1289)
                     /locus_tag="PKV23_05465"
     CDS             complement(<1..1289)
                     /locus_tag="PKV23_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018898630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HQX84919.1"
                     /translation="MRDDLTSAPAPTGSKWRQARGEPSLADVFRTIKIKPSSGMFRRA
                     MAFVGPGYLVSVGYMDPGNWATSIAGGAQFGYALLFVALLSNIMAIILQSLCARLAIG
                     SRRDLAQACRDAFPRSVSFVLWIFAEIAIIATDIAEVVGTAIGLNLLFGIPLELGVII
                     TALDVFIILWLQRLGFRWVEAFIISLLGVIFVCFFLQIAMADPDWGGVIKGFAPTVDI
                     VGIPQMLYLALGIIGATVMPHNLYLHSGIVQTRDYGDSLPEKREALKFATWDSTVALM
                     FALLVNASILILAAATFYKTGRTEVSELGDAHAMLAPLLGSLLAPKLFAISLLACGLN
                     STVTATLAGQIVMEGFLNIRLPAWLRRLATRMVAIVPAAGVTIYYGAEGTGTLLILSQ
                     VILAFQLPFAIVPLVMFTRDKAKMGALVAPGWLTVVTG"
     gene            complement(1291..2433)
                     /locus_tag="PKV23_05470"
     CDS             complement(1291..2433)
                     /locus_tag="PKV23_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018238741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal transporter"
                     /protein_id="HQX84920.1"
                     /translation="MGSRHFWLALPLVVTVAIVALLLIWQPLDRLSAEAPPVEEAAIE
                     RVNLTPGLISLDVRTDGSEPVVIAQVQVDTAYRLFAAAPLLGGARLGLTRIDIPYPWI
                     EGEAHHIALLTSTGAIIDHSIEVATATPVLSGPSLGLLAAVGLLLGAVPVATGLLAWP
                     AMRSLSRPTMNFLLALTVGLLVFLLIDTIGEGLEAGAETIGRLRGTALFWVMLAVTTL
                     VLLALGRAKGAAPEGLRLAAFISLGIGLHNLGEGLAVGAALATGSAALATFLVIGFTI
                     HNVTEGIGIAVPIADERPKFMQFAGLAALAGFPAIVGTIAGTQAVSPLWIAVCFGVGA
                     GAILQVIIEVGALIARRAGPGQWLSPPVAAGVSAGLGVMYATALLV"
     gene            complement(2433..>2900)
                     /locus_tag="PKV23_05475"
     CDS             complement(2433..>2900)
                     /locus_tag="PKV23_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006206024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HQX84921.1"
                     /translation="DPDPALHPEFEAVAKSRLLGTPENAQWQEMVMVMNAFDTNFDDE
                     NEFYCVNTIPFAYFDKPIRVEKSRPIRIYLVNITEFDPINSFHVHANFFEFFDHGTTL
                     TPTSRTVDTVMMCQAQRGILEMTFAEHEPGIYMFHAHQSEFAELGWMSHFEVV"
BASE COUNT          650 a          945 c          858 g          447 t
ORIGIN      
        1 cccgtcacca ccgtcagcca gcccggcgcc accagcgccc ccattttggc cttgtcgcgg
       61 gtgaacatta ccagcggcac aatggcaaag ggcaactgaa acgccaatat aacctggctg
      121 aggataagca gcgtgccggt gccctctgcg ccataataga tggtcacgcc cgccgccggc
      181 acaatcgcca ccatgcgcgt cgccagccgg cgcagccagg cgggaaggcg gatattgagg
      241 aagccttcca tcacgatctg ccccgctagc gtggccgtca ccgtcgaatt gaggccgcag
      301 gccagcagcg aaatggcaaa gagctttggc gccagcagcg aacccaacaa gggcgccaac
      361 atggcatggg cgtcgccaag ttcagaaacc tcagtgcgcc ccgtcttgta gaacgtcgcc
      421 gccgccagaa tgagaatcga ggcattgacc agcagcgcaa acatcagcgc cacggttgaa
      481 tcccacgtgg caaatttcag cgcttcgcgt ttctccggca ggctgtcgcc atagtcccgc
      541 gtctgcacga tgcctgaatg cagataaaga ttatgcggca tgaccgtggc cccgatgatg
      601 cccagcgcca gataaagcat ctgcggaatg cccacgatat ccaccgtcgg cgcaaacccc
      661 ttgatcaccc cgccccagtc cggatcggcc atggcaatct gcaggaagaa gcagacgaag
      721 atcaccccca gcagcgagat gatgaaggcc tcaacccagc ggaagcccag ccgctgcagc
      781 cagaggatga tgaacacgtc gagcgccgtg atgatcacac cgagttcaag aggaatgccg
      841 aacagcaggt tgaggccaat ggcggtgccg acaacttcgg caatatccgt cgcaataatg
      901 gcgatctccg cgaaaatcca cagcacgaag gaaacgctcc tgggaaacgc gtcccggcag
      961 gcctgggcca ggtcgcgcct cgagccgatg gccagtctgg cgcacagcga ctgcaggatg
     1021 atggccatga tgttggagag cagcgccacg aacagcagcg cgtagccgaa ttgcgcgccc
     1081 ccggcgatgg aagtcgccca gttgcccggg tccatatagc ccaccgaaac caggtagccc
     1141 ggcccgacaa aggccatggc ccggcggaac atgccgctcg agggctttat cttgatggtg
     1201 cggaaaacat cggcaagcga aggctcgccg cgcgcctgcc gccatttgga acccgtgggc
     1261 gccggggcac tggtcaaatc gtctcgcatg tcagaccagc agcgctgtcg cgtacatcac
     1321 gccaagtccc gcggaaaccc cggcggcaac cggcggcgaa agccattgtc ccgggcccgc
     1381 gcgccgcgca atcagcgcgc ccacttcgat gatgacctgc aggatggccc ccgccccaac
     1441 gccgaagcac acggcaatcc acaaggggct cacggcttgc gtgccagcta tggtgccaac
     1501 gatggcgggg aatccggcca gcgccgcaag gccagcgaac tgcatgaatt ttggccgttc
     1561 gtcggcgatc ggcacggcga tgccaatacc ttccgtcaca ttgtgaatgg taaagccaat
     1621 gaccaggaat gtcgccagcg ctgccgaccc cgtggcaagg gccgcaccga cggccagccc
     1681 ctcgcccagg ttatgcaagc cgatgccaag ggaaatgaag gccgccagcc gcaggccttc
     1741 cggcgccgcg cccttggccc gccccagggc cagcagaaca agtgtggtca ccgccagcat
     1801 cacccaaaac aaggctgtgc cccgcaagcg cccgatggtt tccgcccctg cttcaagccc
     1861 ctcgccgatt gtatcgatca gcaggaaaac cagcaggcca accgtgagtg ccagcaggaa
     1921 attcatcgtg ggccgcgata gagaacgcat ggcgggccag gccagcaacc cggttgccac
     1981 tggaaccgcg cccagcaata gtcccaccgc cgccagaagc ccgagcgacg ggcccgacaa
     2041 aaccggcgtt gccgttgcaa cctcgatcga atggtcgatg attgcacccg tagatgtcag
     2101 cagggcgata tgatgggctt cgccctcgat ccatgggtag ggaatgtcaa tgcgcgtaag
     2161 cccaagccgc gctccgccca aaagcggtgc cgcggcaaac aggcggtagg ccgtgtcaac
     2221 ctgcacctgg gcaatcacca ccggctccga accatctgtc cgcacatcaa gcgaaatgag
     2281 gcccggcgtc agattcaccc gctcgatggc cgcctcttca accggcggtg cttccgccga
     2341 aagccggtca agcggctgcc agatcagcag aagcgccaca atcgcaaccg tgacgacgag
     2401 tggcagcgcc agccagaagt gcctggagcc catcacacca cctcgaagtg gctcatccag
     2461 ccgagttctg cgaactccga ctggtgggca tggaacatgt agatgccggg ctcatgctcg
     2521 gcgaaggtca tttcgaggat gccgcgctgc gcctggcaca tcatcaccgt gtcaaccgtg
     2581 cgcgatgttg gtgtgagcgt cgtgccgtga tcgaaaaact cgaagaaatt cgcatgcaca
     2641 tggaacgagt tgattggatc gaactcggtg atgttgacca ggtaaatccg gatgggccgc
     2701 gacttctcga cccggatcgg cttgtcgaaa taggcaaacg gaatggtgtt gacgcaataa
     2761 aattcattct catcgtcgaa gttggtgtcg aaggcgttca ttaccatcac catttcctgc
     2821 cactgcgcgt tctccggcgt gccgagcagt cgtgacttgg cgaccgcctc gaattccggg
     2881 tgcagagccg gatcgggatc
//
LOCUS       DAORVN010000225         2896 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_31294_length_2896_cov_3.577262, whole genome shotgun sequence.
ACCESSION   DAORVN010000225 DAORVN010000000
VERSION     DAORVN010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2896)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2896)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2896
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31294_length_2896_cov_3.577262"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(35..499)
                     /locus_tag="PKV23_05480"
                     /pseudo
     CDS             complement(35..499)
                     /locus_tag="PKV23_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013503215.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
     gene            803..2332
                     /locus_tag="PKV23_05485"
     CDS             803..2332
                     /locus_tag="PKV23_05485"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HQX84922.1"
                     /translation="MPLAPRSQRLRNAQTTRAERIKRRKFRRNLFTFIAAQLVIAGVA
                     FVVVRDYLSDGHIKHAVADVLKQVAGSGDQSKANPDLATAPPSQIATANQQTVAVQQA
                     TGEFESEAAPPAETQIALLPAESGSTAVEQNASTSVEPEPEPQAEVQAQATQEDPDPE
                     PQVEAQAAPAAIELEPEPAPEPQPAPVVAAPAPAAAVAEPAPVAQQVAAALPVPNLAN
                     LKNAPSVIEQPGFADSQQKEGLVFRDCDICPELVSVAPGEFMVGPEGRGTAQQAITAV
                     DAKRVVVDKPFAIGRYEVTFEEWQHCVDAGGCKVQPPDDGWGRGRHPVIHVSYNDISE
                     QYLPWLSKVTGFTYRLPTEAEWELASQGGNKSSRGGISETGSNVQLACSYGNSSDVAA
                     KMRETIWAGAACNDGFLYTAPAGSLSPNSLGIYDMHGNVWEWVSDCWRATYDTEETGN
                     SSNCKYHVLRGGSWASEFRFLPDGVWIGDAVAYRAAVRGWETSVKARNSIGFRVARSQ
                     P"
     gene            2369..>2896
                     /locus_tag="PKV23_05490"
     CDS             2369..>2896
                     /locus_tag="PKV23_05490"
                     /inference="COORDINATES: protein motif:HMM:NF013625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HQX84923.1"
                     /translation="MKKHFFISLSLTCAMALFAASATPLYAQGMISAPSLESDLSEAG
                     LDGLVELDAQEVSTIQRLLRRLGYLKTENMTRKMDAATTAALVAHFQAENTSADGLSA
                     DKIMRSLFTAAWTKEGWATGSADGQSLVVDRAEVRQAQDALKKLNYAPGPSDGVFGPA
                     TLSAIEIFQEDSGMNV"
BASE COUNT          612 a          828 c          885 g          571 t
ORIGIN      
        1 cgctcagcga gcatgtcctg actcaatccg gccgcgagcc ggtattgccg caggggaagg
       61 cgatgaggtt ctgcgtggcg tagaactcgt tcatcagctc catgccgccg ccatggtaca
      121 tccaggcatt ctgctggcgt gaattcaggc cgaagggcac agccgtgccg aaggcgtagg
      181 ttgggtcctt gccccaatag tagtacgagg ccgtgtggca catttccaca ttgcccttgc
      241 ccacctcatt ggcggcttca aggggagggg ctatttcgcc gccggggaaa acctgaatct
      301 ggaacttgtt gtcggttgcc tctgcgacaa atttcgagaa agtctcagcc gcgccataaa
      361 ttgtatcgag cgccttggga aagcttgagg tcatgcgcca tttaagttcc ggcatcgtct
      421 gggcgatggc gggcgacgcc aaggccggta ctgcggcggc cgcgatgccc gcttttctca
      481 taaatccacg acgatccact caagtcctcc cattgcttcc ctgttgtaga cgagcgaagc
      541 ctaactgaag tctgggacga gggcaattgg cctgaatcag gctgccagca atgccggaac
      601 aggtgatccg gaaaattgat acgtgtacgg attaattata tttttttgaa aatggttaac
      661 ggcaactgag gcgcgggggc cacaattacg ccgcattgaa gcctaatatt tttctgcggg
      721 acgagcgaca aaagaaccgc gggtggcacc acggttcaaa aatgcggttg atgatttgtc
      781 tgctccacat acggatacac ccttgccttt ggcgccgcgt tcgcagcggt taaggaatgc
      841 gcaaacgact cgcgccgagc gtattaagcg gcgcaagttc aggcgcaatc ttttcacctt
      901 catcgccgcc caactggtga ttgccggcgt ggcttttgtg gttgtgcgcg actatttatc
      961 tgacggccat atcaagcatg cagttgcaga tgtcttgaaa caggttgcag gctccggcga
     1021 tcaaagcaag gcaaatccgg acctggccac tgccccgcca tcccagattg cgaccgccaa
     1081 ccagcagact gtggcagttc aacaggcaac tggtgaattc gaatcagagg cagcgccgcc
     1141 ggccgaaact caaattgcgc ttttaccggc agaatccggg tccactgcag ttgaacagaa
     1201 tgcttcgaca tcggttgaac cggaacctga accccaggcg gaagtgcagg ctcaagccac
     1261 acaggaagat ccggaccccg aaccccaggt ggaggcccag gcggcgccgg cagcaattga
     1321 attggaaccg gaacccgcgc ccgaaccaca gccggctccg gtagttgcgg cacccgcacc
     1381 ggctgccgct gttgcggagc ctgccccagt ggcacagcaa gtggcggcgg ctctgccagt
     1441 tcccaacctc gcaaacctga aaaatgcccc atcggtgatt gaacagccag gcttcgccga
     1501 ttcacagcag aaggaggggc tggtgttccg ggattgcgat atttgccccg agcttgtttc
     1561 ggtcgctccg ggcgaattca tggttggccc ggagggccgc ggcacggcgc agcaggcgat
     1621 aaccgccgtt gatgcaaagc gggtcgtcgt cgacaagcct tttgccatcg ggcgctatga
     1681 agtcaccttt gaagaatggc agcactgcgt tgacgccggc ggctgcaaag tccagccacc
     1741 cgatgatgga tggggccgcg gacgccatcc ggtcatccac gtgtcctaca atgatatcag
     1801 tgaacaatac ctgccgtggc tgtcgaaagt taccggcttc acctacaggc tgccgacgga
     1861 agcggaatgg gaactggcct cgcagggcgg caacaagtcg agccgcggcg gcatcagcga
     1921 aacgggcagc aatgtgcagc ttgcctgctc ctatggaaat tcgtccgatg tggcggccaa
     1981 aatgcgcgag accatctggg caggcgctgc ctgcaatgac gggtttctct acaccgcgcc
     2041 ggcgggttcg ctgagcccca attcccttgg aatttacgac atgcacggca acgtctggga
     2101 gtgggtgtcc gattgctggc gcgccaccta tgacaccgaa gagacgggca attcctcgaa
     2161 ctgcaagtac catgtcctgc gcggcggctc ctgggccagc gagttccgct tcctgccgga
     2221 tggcgtctgg attggcgatg ctgttgccta tcgcgcggcg gtgcgggggt gggaaacatc
     2281 ggtcaaggcc cgcaactcaa tcggcttccg tgtcgccagg tcgcagccgt aaagtaaaaa
     2341 acaaacaaaa aatggggagt ccgtcaccgt gaagaagcat tttttcattt ctttatcgct
     2401 cacctgtgca atggcgttgt ttgccgccag cgccacgccg ctatatgcac agggcatgat
     2461 ttccgccccg tcgctggaaa gcgatctgtc ggaggcgggg cttgacggcc ttgtggaact
     2521 tgatgcccag gaggtgtcca caatccagcg cctgctgcgg cggcttggtt acctcaagac
     2581 ggaaaacatg acgcgcaaga tggatgccgc cacaactgcg gccctggtgg cgcattttca
     2641 ggcggagaat acctccgccg atggcctgtc ggcggataaa atcatgcggt cgctgtttac
     2701 cgccgcctgg acaaaggaag gctgggccac gggaagtgct gatggccaga gcctcgtggt
     2761 ggaccgggcg gaggtgcgcc aggcccagga tgcgctgaag aagctcaatt atgcgccagg
     2821 gccatcggac ggggtgttcg gcccggctac gctttcggcc atcgagatat tccaggagga
     2881 cagcggcatg aatgtt
//
LOCUS       DAORVN010000226         2871 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_31771_length_2871_cov_2.168679, whole genome shotgun sequence.
ACCESSION   DAORVN010000226 DAORVN010000000
VERSION     DAORVN010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2871)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2871)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2871
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31771_length_2871_cov_2.168679"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(57..890)
                     /locus_tag="PKV23_05495"
     CDS             complement(57..890)
                     /locus_tag="PKV23_05495"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HQX84924.1"
                     /translation="MDLELKGKTIIITGGSGGIGQGLVMEFAREGCNVVSASRDAATG
                     QKLAEEAKHQGLSGQVLAVATDVTQRASVEAMIEKAKQRFGPIDGLVNNAGGVAHPAA
                     FAELDEEARRWEIALNIDGVVNCCQAVAKDMLNLGRGSIVNISSNSSLLGEAAAGVAH
                     YGATKGFVNSLSKALAWEWAKQGVRVNNICPGWIVPHKAENVGGGSFWNRFGFEQLGK
                     PEDMERARQEGSLFNMSGLPTPRLGRPEDIANLALFLASDLSRWMSGAELVADGAMTV
                     S"
     gene            complement(911..1687)
                     /locus_tag="PKV23_05500"
     CDS             complement(911..1687)
                     /locus_tag="PKV23_05500"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQX84925.1"
                     /translation="MACLANKVAIITGAGSGIGRACALRFAAEGACVVVNDIAIAGAA
                     ATVDKIISDGGRAVAHGADVTSAAAVQALVDDALTRFGRLDIMFSNAGGGRPTPLHQI
                     DPDEYRRLMGLNLDSVYHSVHAALPVMIRQGSGVFLSTTSGAGLAAVPGLAIYGAAKA
                     GIINLMRSVAVEYGPQGIRANTISPGPMDTPALRAWLDTTSGGAVRYAQQIPLRRLGT
                     AEDIARAAVFLASDAASFISGAVLPVDGAIHARLASPNND"
     gene            complement(1687..>2871)
                     /locus_tag="PKV23_05505"
     CDS             complement(1687..>2871)
                     /locus_tag="PKV23_05505"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HQX84926.1"
                     /translation="LSVSSLVILSRHAQELLDTAMANTGLSDFGKDEWREPFEILVKS
                     INEEAELNLFGRLMTRSDLLIWLQERLEIEETYRRHPEIEDEVVDAPVFIVGQARSGT
                     SILFELLSQDGQFGVPTNWEIMFPCPPPEAATYRSDPRIAKAQHLLTQWHRVAPSFLA
                     MHELGASIPNECKVAMNCTFVTDNLTGIFQIPSYYQWLTQADLSVPYAYYKRMLKLLQ
                     WKNPRSHWLLKSPSHTESLPVLFKVFPDAQVVYTHRDPIKARASVTNLLGTLYWMRSD
                     KPFDATAFERLMTPEAYAHSLDRIIEQIEDGTIPRSRIHDLLFADLMAQPQETIVRLY
                     QQMGLQLSEQASSSVLNYLSQKPQGKFGKHQYSVGERDQIARERKIFVRYQQFHHVPD
                     EV"
BASE COUNT          518 a          871 c          911 g          571 t
ORIGIN      
        1 tcatcacatg catggtgtgg atctcttcgc ttgctgttgg cggtggtaaa tcggattcag
       61 ctgaccgtca tcgcgccatc ggctacgagc tcagcgcctg acatccagcg cgacaggtcg
      121 gaggcgagga acagcgccag gttggcgata tcctcggggc gccccaggcg aggggtgggc
      181 agaccgctca tgttgaacag gctgccctcc tggcgcgctc gttccatgtc ctctggtttg
      241 cccagctgct caaagccgaa gcggttccag aagctgccac cgcctacgtt ctccgccttg
      301 tggggcacga tccagcccgg acagatgttg ttcacgcgca ccccctgctt ggcccactcc
      361 caggccaacg ccttggacag gctattcacg aaacccttgg tggcgccata gtgggcgacg
      421 cccgcggcgg cctcgcccag cagggaggag ttggaggaga tgttgacgat gctccctcgg
      481 cccagattca acatgtcctt ggcgaccgcc tggcagcaat tcacgacgcc atcaatgttg
      541 agcgcaattt cccagcgtcg agcctcctcg tccagctccg caaaggcggc aggatgggca
      601 acgccgccgg cattgttcac cagcccgtcg atcggaccga accgctgctt ggccttttca
      661 atcatcgcct ccacgctggc ccgttgggtg acgtcggtgg ccactgccag cacttgaccg
      721 gacaggccct ggtgcttggc ctcctcggcg agcttttggc cggtggcagc atcccttgag
      781 gcgctgacca cattgcagcc ttcgcgggcg aattccatca ccaagccctg gccgataccg
      841 ccgctgccac ctgtgatgat gatggtcttg cctttcaatt ccagatccat gcccgttctc
      901 ctcgtcgatg tcagtcgttg ttggggctgg ccagtcgcgc atgaatcgcg ccgtctactg
      961 gcaacactgc cccgctgatg aaactggccg catcgctagc aagaaagacc gcagcgcggg
     1021 ctatatcctc cgccgtaccg aggcgtcgta gggggatttg ctgggcgtac cgaacggcgc
     1081 ccccggatgt cgtgtccagc caagcccgca gtgccggcgt atccatcggg ccgggggaaa
     1141 tcgtgttggc gcggatgccc tgggggccgt attcgacggc gacgctgcgc atcagattga
     1201 tgatgccggc cttggccgcg ccatagatgg ccaatcctgg cacggcggcc aagccggcgc
     1261 cggaggtggt gctcagaaat accccgctgc cttgcctgat catcactggc aaagcagcgt
     1321 gcacgctgtg ataaaccgag tccagattaa gccccatcag acgtcgatac tcgtcaggat
     1381 cgatctggtg cagcggtgtc ggcctccccc cgccggcatt gctgaacatg atgtcgagtc
     1441 gtccgaatcg ggtcagagcg tcgtccacca gcgcttgcac cgctgcggct gaggtgacgt
     1501 cggcgccatg ggcgacggcc ctgccgccat cggaaatgat tttgtccacg gtggccgcgg
     1561 cgcccgcgat ggcgatgtcg ttgaccacca cgcaggcacc ttccgcggcg aagcgcaggg
     1621 cgcaggccct gccgatgccg ctgccggcac cggtgatgat ggcaaccttg ttcgcaaggc
     1681 aggccatcag acttcgtccg gtacgtggtg gaactgctga tagcggacaa aaatcttccg
     1741 ttccctggcg atctggtcgc gttcgccgac gctgtactgg tgcttgccga atttgccttg
     1801 aggcttttgg gacaggtagt tgagcacgct gctgctggcc tgctcagaga gctgcaggcc
     1861 catctgttga tagagccgga cgatggtttc ctggggctgt gccatcaggt cggcgaacag
     1921 gaggtcatgg atgcgggatc ggggaatggt gccgtcctcg atctgctcaa tgatccggtc
     1981 gaggctgtga gcataggctt ccggcgtcat tagacgctcg aaggcggtgg catcgaaagg
     2041 cttgtcgcta cgcatccagt agagggtgcc gagcagattg gtgaccgaag cccgggcctt
     2101 gatcggatcc cggtgcgtat agaccacctg ggcatcagga aacaccttga acagcacagg
     2161 caggctctcg gtatgggagg gcgatttcaa gagccaatga gaacgagggt tcttccattg
     2221 cagcagcttc agcatgcgct tgtagtaggc ataggggacg gagaggtccg cctgcgtcag
     2281 ccactggtaa tagctgggaa tctggaagat gccggtcagg ttgtcggtga cgaaggtgca
     2341 attcatggcc accttgcact cgttgggtat gcttgcaccc agctcgtgca tggcgagaaa
     2401 gctgggggcc acccgatgcc actgggtcag caggtgctgg gccttcgcga tgcgcgggtc
     2461 gctgcggtag gtagcggcct ccggtggcgg acagggaaac atgatttccc agttggtcgg
     2521 gacgccaaac tgcccgtcct gtgaaagcaa ctcgaacaga atggaagtgc ctgagcgggc
     2581 ctgaccgaca atgaatacgg gcgcatccac gacctcgtcc tcgatctccg gatgacggcg
     2641 gtaggtttcc tcgatctcca gccgctcctg aagccagatc agcagatcgc tacgcgtcat
     2701 caggcggccg aaaaggttga gctccgcctc ctcgtttatt gacttgacca gaatttcaaa
     2761 gggctcgcgc cattcgtctt tgccgaagtc ggagaggccc gtgttggcca tcgccgtgtc
     2821 cagcagttcc tgggcgtggc ggctcaagat gaccaggctt gaaacactga g
//
LOCUS       DAORVN010000227         2870 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_31788_length_2870_cov_2.808171, whole genome shotgun sequence.
ACCESSION   DAORVN010000227 DAORVN010000000
VERSION     DAORVN010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2870
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31788_length_2870_cov_2.808171"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..278
                     /locus_tag="PKV23_05510"
     CDS             <1..278
                     /locus_tag="PKV23_05510"
                     /inference="COORDINATES: protein motif:HMM:NF014896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HQX84927.1"
                     /translation="ADPHLWLEEVEGERALAWVHQQNDRSLPVLENDPRYAARLSAAL
                     EVAESRDRLPLGQVRGGYLYNFWQDPDHVRGIWRRAVCSYSGSDKHI"
     gene            217..975
                     /locus_tag="PKV23_05515"
     CDS             217..975
                     /locus_tag="PKV23_05515"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HQX84928.1"
                     /translation="MYAASGGAQYVLTPEVTNIFESDRQRLRAVVAREEEDASVAGFS
                     NLAQELGIWVHAGSLALRLSEDKLVNRSLLFAPDGTVAARYDKIHLFDVDMPSGERIR
                     ESDYFAPGSEAVVAALPWGPLGLSICYDMRFPKLYNVLANAGAMFMAVPAAFTVPTGQ
                     AHWHVLLRARAIETGSFVFASAQGGAHESGRHTFGHSLAVNPWGEIIAELGTEPGVLP
                     FEVDEGFVNRDRGRIPALKNARAFNLKPPQGIAN"
     gene            981..1424
                     /locus_tag="PKV23_05520"
     CDS             981..1424
                     /locus_tag="PKV23_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003533515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1178 family protein"
                     /protein_id="HQX84929.1"
                     /translation="MIRYDLICDKGHEFDGWFRDSAAFDKQVKRHLISCTHCGSDAVS
                     KQLMAPGIPVRSNRKAEASQTMVAGTVDPRTQKLMQMMREMRKSVEANAEYVGDRFAD
                     EARKIHYAESEKRGIYGEAKPEDAKALIDEGIEVHPLPRLPEDSN"
     gene            complement(1421..2164)
                     /gene="ubiG"
                     /locus_tag="PKV23_05525"
     CDS             complement(1421..2164)
                     /gene="ubiG"
                     /locus_tag="PKV23_05525"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008934888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008689 - 3-demethylubiquinone-9
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HQX84930.1"
                     /translation="MTAAAQTTLDPAEVEKFSKMAAEWWNPNGKFGVLHVFNPVRLAF
                     IKEQVCARFGRDPYDPKPFRGLRFLDIGCGGGLLTEPMARLGATILGVDPSEKNILTA
                     RVHAEEQELAIDYRCATAEDLAAAGERFDVILNMEVIEHVANPQGFTRICVEMLNPQG
                     LMFVATISRTLKSFGLAIIGAEYVLGWLPKGTHQWEKFITPAELKTWLADNHVAIKGE
                     TGVTYHPLANEWRRSRDMDVNYMLVGQKN"
     gene            2308..>2870
                     /locus_tag="PKV23_05530"
     CDS             2308..>2870
                     /locus_tag="PKV23_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013651232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HQX84931.1"
                     /translation="MGRLVMKFGGTSVADIGRIRNVARHVLREVKAGNHVAVIVSAMA
                     GTTDQLVAWCKEAAAVHDAREYDAIVASGEQVTAGLLAIILQDMGVPARSWHGWQIPL
                     KTDGVHASARIAEIDAAEIRKRMEQGQVAVVAGFQGIGPDKRITTLGRGGSDTSAVAI
                     AAALDAACDIYTDVDGVYTADPRIVTGA"
BASE COUNT          560 a          771 c          951 g          588 t
ORIGIN      
        1 ccgctgatcc gcatctgtgg cttgaagagg tcgagggcga acgggcgctg gcgtgggtgc
       61 atcagcagaa cgatcgctcg ctgccggtgt tggaaaatga tccgcgctat gcggcgcgct
      121 tgagcgcggc gcttgaagtg gcggagagtc gcgatcgctt gccgctcggg caagtgcgcg
      181 gcggctatct ctataatttc tggcaggacc cggaccatgt acgcggcatc tggcggcgcg
      241 cagtatgttc ttactccgga agtgacaaac atatttgaat cagaccgcca gcgtctgcgc
      301 gccgtggtgg cgagggaaga agaggacgcc agcgttgcgg gcttttcgaa cctggcgcag
      361 gagcttggca tctgggtcca tgcggggtcg ctggccttgc ggctgtcgga ggacaagctg
      421 gttaaccgct cgctgctgtt tgcccccgat ggaacggtgg cggcgcgcta cgacaagatc
      481 catctttttg acgttgacat gccgagcggc gagcgcatcc gggagtcgga ctactttgcg
      541 ccgggaagcg aagcggtagt cgccgccctt ccctgggggc cgttgggcct cagcatctgc
      601 tacgacatgc gttttcccaa actttacaat gtgttggcaa atgcgggtgc gatgttcatg
      661 gcggtgcccg cggcctttac ggtgcccacg ggccaggcgc actggcatgt tctcctgcgc
      721 gcacgggcca ttgaaaccgg aagtttcgtg tttgcgtcgg cacagggcgg ggcccatgaa
      781 agcggccggc acacgttcgg ccacagcctg gcagtcaatc cctggggcga aatcatcgcc
      841 gagttgggaa cggagcccgg ggttttgccg tttgaagttg atgaaggatt tgtaaaccgg
      901 gaccgtggca gaatacccgc acttaagaat gcccgtgcct ttaacttgaa gccgcctcag
      961 ggaatagcta actaagcatc atgatcagat acgacctcat ctgcgacaag ggccacgaat
     1021 ttgatggctg gttccgggac agtgcggcat ttgacaagca ggtcaagcgg cacttgatct
     1081 catgcaccca ttgcggctct gatgcggtga gcaagcagct catggcgccc ggcattccgg
     1141 tgaggtccaa ccggaaggcg gaggcttcgc agacaatggt ggcgggcacg gtcgatcccc
     1201 gcacgcagaa actgatgcag atgatgcgcg agatgcgcaa gtcagtcgaa gccaacgccg
     1261 aatatgtggg cgaccggttt gccgatgaag cgcggaagat ccattacgcg gaaagtgaaa
     1321 agcgcggcat ctatggcgag gccaaacccg aagacgccaa ggccctgatc gacgaaggca
     1381 ttgaagtcca tccactgccg agattgccgg aagattcgaa ctagttcttc tgccccacca
     1441 gcatgtaatt cacatccatg tcgcgtgacc tgcgccattc attggcgagc gggtggtagg
     1501 tgacgccggt ttcgcccttg atggcgacgt gattgtcggc gagccaggtt ttcagttcag
     1561 cgggtgttat gaatttttcc cactgatggg tgcccttggg cagccagccc aggacgtatt
     1621 cggcgccgat gatggcaagg ccgaaggatt tgagggtgcg gctgatagta gccacgaaca
     1681 tcaagccctg aggattgagc atctcgacac agatgcgggt gaagccttgc gggttcgcca
     1741 cgtgctcgat cacttccatg ttgaggatga catcgaaacg ctcgcccgcg gctgccaggt
     1801 cctcggcggt cgcgcagcgg taatcgatgg cgagttcctg ttcttccgca tggaccctgg
     1861 cggtcaggat gtttttttcg gaagggtcga cgccgaggat ggttgctcca agccttgcca
     1921 tgggttcggt caggagcccg ccgccgcagc cgatgtcgag gaagcgcagg cccctgaagg
     1981 gcttgggatc atagggatcg cggccaaatc gggcgcaaac ctgttccttg ataaaagcca
     2041 gccggaccgg gttaaagaca tggagaacgc cgaatttacc gttgggattc caccattcgg
     2101 cagccatttt ggagaatttc tcgacttcgg cgggatcaag ggtggtttgg gctgcggcag
     2161 tcatttcaaa catccatttt tatgggggcc attattgcat aagcaaggac cctcatctat
     2221 gagtacgccg cgtcgcacct aaaaagttca ctttacatct gatttggctg caagccagcg
     2281 tttcgggatc aagaaggatt ttggccgatg ggccgtttgg ttatgaagtt tggaggcact
     2341 tcggttgcgg atatcgggcg tatccgcaat gtggcgcgcc atgtcctgcg cgaggtgaag
     2401 gccggtaacc atgtggccgt cattgtctct gccatggccg ggaccacgga ccagctggtg
     2461 gcctggtgca aggaagccgc cgcggtgcat gatgcccggg aatatgacgc cattgtcgcc
     2521 tcgggcgaac aggtcacggc agggcttctg gccattatcc tgcaggacat gggggttccg
     2581 gctcggtcct ggcacggctg gcagatcccg ctcaaaaccg atggggtgca tgcctcggcg
     2641 cggattgccg aaatcgatgc ggcggagatc agaaagcgca tggagcaggg ccaggttgcg
     2701 gtggttgccg ggttccaggg catcgggccg gacaaacgca tcaccacgct ggggcgcggc
     2761 ggctcggata cgtcggcggt ggcgattgcc gcggcgcttg acgcggcctg cgacatttat
     2821 acggatgtgg acggggttta cacggccgat ccccgaattg tgacgggggc
//
LOCUS       DAORVN010000228         2856 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_32076_length_2856_cov_2.990718, whole genome shotgun sequence.
ACCESSION   DAORVN010000228 DAORVN010000000
VERSION     DAORVN010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2856
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32076_length_2856_cov_2.990718"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            35..109
                     /locus_tag="PKV23_05535"
     tRNA            35..109
                     /locus_tag="PKV23_05535"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:67..69,aa:Thr,seq:ggt)
     gene            complement(134..784)
                     /locus_tag="PKV23_05540"
     CDS             complement(134..784)
                     /locus_tag="PKV23_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003546160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HQX84932.1"
                     /translation="MKDFKINPEFAITDEPALRSLFEATHALATLKCQGSLDRHAQDF
                     IRRSPFLCIGTQDRNGKADVSPRGDPVGFVKILDQHTLAIPDRPGNNRLDTLVNILAN
                     PSVGLLFIIPGFDDTLRVSGQASLVNDPEILKSMSIKDRIPTLAIVVKVSEVFMHCAK
                     AFRRSQLWNPEHFQDRREMPSLSKIILDQTTGAPTDDEAMRKLDDELEDDYKRTLY"
     gene            complement(863..1387)
                     /locus_tag="PKV23_05545"
     CDS             complement(863..1387)
                     /locus_tag="PKV23_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018390052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HQX84933.1"
                     /translation="MIDGLVLLAAVAFVLAAMKHFGWLDLGTGTLQVVDGDSLRRGDL
                     DIRLHGIDAPEYRQTCRDKHDSEYPCGKQAANALRGLVGKQEVSCRSIETDRYGRSVA
                     VCRIGGLEINGEMVRLGWAVAYTRHSLSYVRMEAEARRARRGIWAGSFEPPEDYRARL
                     HLVQGNLGDSPPPD"
     gene            complement(1426..1884)
                     /locus_tag="PKV23_05550"
     CDS             complement(1426..1884)
                     /locus_tag="PKV23_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015318468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HQX84934.1"
                     /translation="MIRVQAQAFDTAAELAKLKQGRTDIGGSVVFVGTMRDMSDGAAV
                     SAMTLEHYPGMTEKALAEIDAEAQRRWPLLDTLIVHRHGKLLPGDDIVLVIATSKHRE
                     AAFDACHFLIDWLKTKAPFWKLESGAQGDTWVSAKSSDDESASRWDKTPS"
     gene            complement(1916..>2856)
                     /locus_tag="PKV23_05555"
     CDS             complement(1916..>2856)
                     /locus_tag="PKV23_05555"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HQX84935.1"
                     /translation="STDIFSLDPYLPPQKASISGAGKEDTGWDLSPVSFSGLNDVEGD
                     VKLSVFQVKWGDAKFGPAELSGTLKGGKLQTAIDDASLYGGKATVEITLDGRPEPPAL
                     HLVLDAQGMKGETFLSEFAGVEWLAGTTALKASLSATGHNQREMMSTLAGTFSIDVAD
                     GEITGLNIVDMVSKLNTALSDGWGRGPENLTSFDSAQASFVIGDGIGKTTDVMVSGRT
                     FAVTGGGEIDMLRRALDFKFDPRLVTGDAESTGLPVEIAVSGPWQAPKIYPDVEGIFD
                     DPEAAYEALRGLGVSGKTMKKIGKKGKKLLEDLFGN"
BASE COUNT          548 a          884 c          804 g          620 t
ORIGIN      
        1 cggtacggtt ccggctcttt cttgtctggc gttcgctgcc gtagctcagt ggtagagcac
       61 tccattggta atggagaggt cgacagttca atcctgtcta gcagcaccat ttttcccaaa
      121 cattctgctg gacctagtaa agcgtcctct tgtaatcgtc ctcgagttca tcatccagtt
      181 tgcgcattgc ctcgtcatct gttggagcgc cggttgtctg gtccaggatg atctttgaga
      241 gtgacggcat ctcgcgtcga tcctgaaaat gctcggggtt ccacaattgc gagcgccgga
      301 atgccttggc gcaatgcatg aagacctcgc tcaccttgac aactatggca agtgtgggaa
      361 tgcggtcttt gatgctcatg ctcttgagga tttcagggtc attcaccagg ctggcctgcc
      421 cactcacccg caacgtatcg tcaaaccccg gaatgatgaa aaggagtccg acgctggggt
      481 tggcgagaat gttgaccagg gtatcgagac ggttgttgcc cggccggtcc ggtatcgcca
      541 gcgtgtgctg atcaaggatt ttgacaaatc ccaccgggtc gccgcgcggg ctgacatcgg
      601 cttttccatt ccggtcctgt gtgccgatgc agagaaaggg cgaacgcctg ataaagtctt
      661 gcgcatgcct gtcgagtgag ccctggcact tcaaggtggc cagggcatgc gtcgcctcaa
      721 acaggcttcg cagcgcgggc tcatcggtta tagcgaattc cggattgatc ttgaagtcct
      781 tcatactgtt gcctttcgaa gcgatcttta cagttagcgc caatgactgg ctagattcaa
      841 tcctggccgg cagcaccatt tctcaatccg gcggcggcga atcccccaaa tttccctgaa
      901 ccagatggag tcgagcgcga taatcctcgg gcggctcgaa gcttcccgcc caaatccccc
      961 gcctggcgcg ccgcgcctcg gcttccatcc gcacatagga aaggctgtgg cgggtgtagg
     1021 caacagccca acccagccgc accatttccc cgttgatttc aagcccgcca atccggcaaa
     1081 ccgcaacgga gcggccataa cggtccgttt caatcgacct gcaggaaacc tcctgcttgc
     1141 caaccaggcc gcgcagggca ttcgcggcct gcttgccgca gggatattca ctgtcatgct
     1201 tgtcccggca ggtctgccgg tattccggcg catcgatgcc gtgcaggcgg atatccaaat
     1261 cgccgcggcg cagcgaatcg ccatcgacca cctgaagtgt tccggtccca agatcaagcc
     1321 agccgaagtg tttcatggcc gccagcacaa aggcgacggc agcgagcaag acaaggccgt
     1381 cgatcatgct cttgtaaaac ggccgggggc ctcgccggta acccctcacg aaggcgtctt
     1441 gtcccagcgt gatgcacttt cgtcgtcgga ggatttggcc gaaacccagg tgtcgccctg
     1501 tgcgccgctt tccagtttcc agaacggcgc cttggtcttg agccagtcga tcaggaaatg
     1561 gcaggcgtcg aatgctgcct cacggtgctt tgatgtggcg atgaccagga caatgtcgtc
     1621 tcccggcagc agcttgccgt ggcgatggac gatgagcgta tcgagcagcg gccagcgccg
     1681 ctgggcctcc gcatcgattt ccgcaagcgc cttctccgtc atcccgggat agtgctcaag
     1741 ggtcatggcg gaaactgccg ccccatcgct catgtcgcgc atggtgccca caaaaaccac
     1801 gctgccgcca atatccgtcc ggccctgctt gagctttgca agttcggcgg cggtatcgaa
     1861 ggcctgggcc tggacgcgga tcatggtgca ataatgctct ttgcggcggg cgacttcaat
     1921 tgccgaacag gtcttcgagg agcttcttgc ccttcttgcc aatcttcttc atggtctttc
     1981 cggaaacccc aaggccgcgc agcgcctcat aggcggcctc cggatcgtca aaaatccctt
     2041 ccacgtcggg atagattttt ggcgcctgcc agggcccgct caccgcgatc tcaaccggaa
     2101 ggccggtgct ctcggcatcg ccggtcacca gcctgggatc gaatttgaaa tccagcgcgc
     2161 gccgcagcat gtcgatctcg ccacctcccg tgaccgcaaa ggtccggccc gaaaccatca
     2221 catccgtggt cttgccgatg ccgtcgccga tcacgaagct tgcctgcgcc gaatcaaatg
     2281 acgtgaggtt ttccggcccg cggccccagc catcgctcag tgccgtgttc aattttgaaa
     2341 ccatgtcgac aatgttgagg ccggtgattt cgccatccgc aacgtcaatg ctgaacgtgc
     2401 cggcgagcgt tgacatcatt tcccgttgat tgtggcccgt ggcgctgagg gaagccttga
     2461 gcgccgttgt gccggcgagc cattccaccc ctgcgaattc actgagaaag gtttcgccct
     2521 tcatcccctg ggcatcgaga accaggtgca gggcgggcgg ctccggtctg ccatcaagcg
     2581 tgatttcaac ggtagccttt ccgccgtaca ggcttgcatc gtcaatcgcc gtctgcagct
     2641 ttcccccctt cagggtgccg gacaactcgg ccggcccgaa tttggcatcg ccccatttga
     2701 cctgaaaaac cgagagcttc acatcgcctt ccacatcgtt aagcccggaa aaggagaccg
     2761 gtgacaaatc ccagcccgtg tcttcctttc ctgctccgga gatgctggct ttctgcggcg
     2821 ggagataggg gtcgagcgag aaaatatctg ttgaaa
//
LOCUS       DAORVN010000229         2852 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_32173_length_2852_cov_2.761888, whole genome shotgun sequence.
ACCESSION   DAORVN010000229 DAORVN010000000
VERSION     DAORVN010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2852
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32173_length_2852_cov_2.761888"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2580
                     /locus_tag="PKV23_05560"
     CDS             <1..2580
                     /locus_tag="PKV23_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017957519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HQX84936.1"
                     /translation="ATDESQHVFVDTPEPESDLAKAGGFFRQSWGAQIIELQRYARSG
                     NEKRIHFARDEGTIPSAEQLENMGIKIFVGVGHETTGATTYTLSDITNLLDWIERNPA
                     EHHAVLDATSMLGAMPWGEAIVRRVVSECCLFMPFQKAIGGISGYYVASFTPQALALI
                     EANQKNPSWAIPRQLKIVAPLDAKRPLTGARSVNAGPIYDPAQDKMLGGVINTYSTLA
                     FAETTFGLLRSERRLGPVSELNRRSIANRTAINEWVAAHPLLELGVAEPERRGAAVTL
                     LKVNDKDISDAAIHARIIARSKQILGYEGLTHPDGSHERGLDVARYVNAFPGTPGDYR
                     AWIGGIRAPEDVTALLDNLHYAYLRAKILVLEEELAALGEKFPPPNDAGGRQRADDPT
                     RAYKILVCDLVGLKPGKDGKPDHSAVRKHIEAKGGVFHEGPLGDEGKLAKGKLHFFYQ
                     PQLGTEAELLPITNAGQYDAVIAAATHLPKGSVFKLGGVRIGAGTGNMACATWGGGNG
                     EGGEAVLMNTPSFNSRATAQMVMKALLKVAPDLAVDELHARVVAGDFDTARHLKDFPT
                     EKLEGKKIAIIGYGNIGREVAKLCRAFGMKVSIHARPRHQEWIESEGFEFASTAEDAA
                     RGADFISPHTGLGALNAATGKYVNAGVVGAEVLAVMNNGAVVINYDRGEVVDAKALDG
                     ALQSGKVRYACIDADLFRDAKTGALSGPMVPYREMEARHRGKMELLPHAAADTEHLSR
                     VEGAKQAVDQIFDLIQFKAVTNLKGQLPAGYVSAGARTVHGVGAVTGLDLARATEDAE
                     ALAQARRTAEAMAAIWGALSATVNPERRRELIERHGAALVKAGNEHAALLEKLGLQGP
                     YSG"
     gene            2632..>2852
                     /locus_tag="PKV23_05565"
     CDS             2632..>2852
                     /locus_tag="PKV23_05565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX84937.1"
                     /translation="MGELNWLSAAKLSRGFARKKFSPVEVAKACLGQIAKHDHAINAM
                     CRVEEKAALAQAEASAARWKKGRPLGPLDG"
BASE COUNT          570 a          764 c         1015 g          503 t
ORIGIN      
        1 gccacggacg agagccagca tgtgtttgtg gatacgccgg aaccggaatc ggacctggcc
       61 aaggccggcg gatttttccg ccaatcctgg ggagcccaga tcattgaact gcagcgctat
      121 gcgcgcagcg gcaatgaaaa gcgcatccat tttgccaggg acgaagggac gattccttcc
      181 gccgagcagc ttgagaacat ggggatcaag attttcgtgg gcgtgggcca tgaaacgacc
      241 ggggccacga cctatactct gtcggatatc acgaatcttc tggactggat tgaacgcaac
      301 ccggcggagc atcatgcggt gctcgatgcc acgtcgatgc tgggggccat gccatggggc
      361 gaggcgatcg tgcgccgggt ggtgagcgag tgctgcctgt tcatgccgtt ccagaaagcc
      421 attggcggca tttcgggata ctatgttgcc tccttcacac cgcaggcact ggccctcatc
      481 gaggccaatc agaaaaatcc gtcatgggca attccccgcc aactgaaaat cgtggcaccg
      541 cttgatgcca agcgcccgct gacgggcgcg cgcagcgtga acgcggggcc gatctatgat
      601 ccggcgcagg acaagatgct gggcggcgtg atcaacacct acagcacatt ggcctttgcc
      661 gaaacgactt ttggactgct gcggtcagag cggcggctgg gcccggtttc cgagctcaac
      721 cgacgctcca ttgccaaccg cacggcaatc aatgagtggg tggcggcgca tccgttgctg
      781 gagcttgggg tggccgaacc ggagcggcgg ggcgcggcgg tgacgctgtt gaaggtgaac
      841 gacaaggaca tcagcgatgc ggctattcac gcgcggatca ttgcgcgctc gaagcagatt
      901 ctgggttatg aaggccttac ccatccggac ggttcgcacg aacgcgggct cgatgtggcg
      961 cgctatgtga acgcctttcc gggaacaccc ggcgattacc gggcctggat tggcggcatc
     1021 cgcgcgccgg aagacgtgac tgcgctgctc gacaatctgc actacgctta cctgcgggcg
     1081 aaaatcctgg tgctggagga agagctcgca gcgcttggcg agaaatttcc gccgcccaat
     1141 gatgcgggcg ggcggcaacg ggcggatgat ccgacaaggg cctacaagat tttggtgtgc
     1201 gacctggttg gcctcaagcc cggaaaagac ggcaagccgg atcacagcgc ggtgcgcaaa
     1261 catatcgaag cgaagggcgg ggtgtttcat gagggcccgc tgggtgatga gggaaagctt
     1321 gccaagggca agctgcattt cttttaccag ccgcaactcg gcacggaggc ggaattgctg
     1381 ccgatcacga atgcgggcca gtatgacgcg gtgatcgcgg cggccacgca tctgccgaag
     1441 ggatcggtat tcaaacttgg cggggtgcgc atcggggccg ggaccggcaa catggcctgc
     1501 gccacatggg gcggcggcaa tggcgagggc ggcgaggcgg tgctgatgaa cacgccgagc
     1561 ttcaacagcc gcgccacggc gcagatggtg atgaaggcac ttttgaaagt ggcgccggac
     1621 ctggcggttg atgaattgca tgcccgggtg gtggccggtg attttgatac ggcgcgccac
     1681 ctcaaggatt ttcccacgga aaagctggaa ggcaagaaga tcgccattat cggatacggc
     1741 aacattgggc gcgaagtggc caagctctgc agggccttcg gcatgaaggt ttccatccat
     1801 gcgcggccgc ggcatcagga atggattgaa tcggaaggct ttgagtttgc ttccacagcg
     1861 gaggatgcgg cgcgcggcgc tgattttatc agcccgcata cgggactcgg ggcgctcaat
     1921 gcggccaccg gaaaatatgt gaacgccggc gtggtggggg ccgaagttct ggctgtcatg
     1981 aataacgggg cggtggtgat caattatgac cgtggcgaag ttgttgatgc gaaggcgctg
     2041 gacggggcgt tgcagagtgg caaggtgcgg tatgcctgca tcgatgcgga cctgttcagg
     2101 gacgccaaga cgggggcact gagcggaccc atggtgccct accgcgagat ggaagcgcgc
     2161 caccggggca agatggaact gctgccccat gcggcggccg acacggagca cctgtcgcgc
     2221 gtcgagggcg ccaagcaggc ggtggaccag atttttgacc ttatccagtt caaggcggtg
     2281 accaacctca aggggcagtt gccggcgggc tatgtgagtg cgggggcgcg cacggtgcac
     2341 ggggttggcg cggtgactgg actcgatctg gcccgcgcga cggaggatgc cgaggcactt
     2401 gcccaggccc gcaggactgc cgaagcgatg gctgccatct ggggcgcatt gtcagcgacg
     2461 gtcaatccgg agcggcggcg cgaattgatc gaacgtcatg gggcggccct ggtcaaggcc
     2521 ggcaacgagc atgcggcgct gctggagaag ctggggctgc aggggcccta ttccgggtga
     2581 tgactataac agcgatttct gcctaagctg gctcgttaca tggagtgagt gatgggtgag
     2641 ttgaattggt tgagcgcggc aaagctttcc aggggctttg cgcggaagaa gttttcgccg
     2701 gttgaggtgg cgaaggcctg cctggggcag attgccaagc atgaccatgc aatcaacgcc
     2761 atgtgccggg tggaggagaa ggccgcgctg gcacaggccg aagcaagtgc cgcgcggtgg
     2821 aaaaagggca ggccgctggg gccgctggat gg
//
LOCUS       DAORVN010000230         2849 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_32225_length_2849_cov_3.582677, whole genome shotgun sequence.
ACCESSION   DAORVN010000230 DAORVN010000000
VERSION     DAORVN010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2849
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32225_length_2849_cov_3.582677"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..835)
                     /locus_tag="PKV23_05570"
     CDS             complement(<1..835)
                     /locus_tag="PKV23_05570"
                     /inference="COORDINATES: protein motif:HMM:NF014803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX84938.1"
                     /translation="MPIAKDFEHRLTPVLASIVEHYGTPFHIYDEKGIRETGEALNQA
                     FSGIDGFREFFAVKALPNPRIMQIMKSLGFGFDCSSVAELTLSRQAGVTGEGIMFTSN
                     NTSQADFQAAGADGGCVLNLDDISLVDKVPRMPELICFRYNPGERRTGNSIIGTPVEA
                     KYGVAHDQLLDAYRLAKARGASRFGLHTMLASNDRNYSYMVETARMLLEIVTWISGEL
                     GIRFEFINIGGGLGIPYKPEDAPLDIESMAAEITALFDAFKAQHAYAPKLFVESGRYM
                     TG"
     gene            complement(901..2127)
                     /locus_tag="PKV23_05575"
     CDS             complement(901..2127)
                     /locus_tag="PKV23_05575"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HQX84939.1"
                     /translation="MIRINENYRKLQASYLFSDIAKRVAAFQKANPGKKLIKLGIGDV
                     TRALPDACIKALHAATDEMAGDGTFRGYGPEQGYDFLREAIAKFDFQARGANVQPDEV
                     FVSDGAKCDTGNFQEIFSLDCKVAVTDPVYPVYVDTNVMAGRAGQWNKETGRYDGLFY
                     LDGNRENGFIPALPTQPVDLIYLCFPNNPTGVTATKAQLKQWVDYAKANKALILFDAA
                     YEAFITDDALPHSIYEVEGAREVAVEFRSYSKTAGFTGTRCAYTVVPKECMAYTESGE
                     AVALHALWNRRHTTKFNGVSYPIQRAAEAIYSPQGQAQVKQLVAYYLNNARYIREKMT
                     ALGYACIGGDNSPYIWIDGKSDSWVFFDKLLNEAGVVCTPGAGFGKCGEGYIRISAFN
                     SFENVQEAMERISAVL"
     gene            complement(2398..2625)
                     /locus_tag="PKV23_05580"
     CDS             complement(2398..2625)
                     /locus_tag="PKV23_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011511319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HQX84940.1"
                     /translation="MAKKSKNDNPVYKIVEVVGSSPVSWEDAASSAIASAAKTLRDLR
                     VAEVTKLDMKIENGKVVAYRARVSMSFKYED"
BASE COUNT          618 a          821 c          796 g          614 t
ORIGIN      
        1 ggccggtcat ataacgtccg ctctcgacaa acagctttgg ggcataagcg tgctgtgcct
       61 tgaaggcgtc aaacagcgcc gtgatctccg ccgccattga ttcaatatcc agtggggcat
      121 cttccggttt gtagggaatg cccagcccgc cgccgatatt gatgaactcg aagcggatac
      181 ccagctcgcc cgaaatccaa gtaacaattt ccagcagcat gcgggcggtc tcaaccatgt
      241 agctgtaatt gcgatcattg gaagccagca tggtatggag cccaaaacgg cttgcccccc
      301 tggccttggc gagacggtag gcgtcgagca attgatcgtg ggcgacgccg tacttggcct
      361 ccaccggtgt gccgatgatg ctgttgccgg tacggcgctc cccggggtta tagcggaagc
      421 agatcagctc cggcatccgc ggcaccttgt cgaccaggga gatgtcatcc aggttgagta
      481 cgcagccccc atcggcgcca gccgcctgga aatcggcctg gctggtgttg ttggaggtga
      541 acatgatccc ctcgccggtg acgcccgcct ggcggctgag cgtcagttcg gccaccgagc
      601 tgcaatcgaa gccaaagccc aatgacttca tgatctgcat gatgcgcggg ttgggcaggg
      661 cctttaccgc aaagaactcg cggaatccat cgatgccgga aaaggcctgg ttcagcgcct
      721 cgccggtttc gcgaatgccc ttttcatcat agatatgaaa gggcgtaccg tagtgctcga
      781 caatggaagc cagaacaggt gtcaggcgat gttcgaaatc ttttgcaatg ggcatgttgt
      841 ttcatactct tgtgtattgg acggtgatca agcgttccgg caaaaatgcc agcggcccga
      901 tcaaagtacg gcgctgatgc gctccatcgc ctcttgtaca ttttcaaaac tgttgaaggc
      961 gctgattcga atatagcctt cgccgcactt gccaaaaccg gcgccggggg tgcacaccac
     1021 tccggcctca ttgaggagct tgtcgaagaa cacccaggag tcgctcttgc cgtcgatcca
     1081 gatgtaaggt gaattatcgc caccgatgca ggcataaccc agcgccgtca tcttttcacg
     1141 gatatatctg gcgttattca gatagtaggc aaccagctgt ttgacctgtg cctgcccctg
     1201 cggactgtag atggcttcgg ccgcgcgctg aatcgggtag gagaccccgt tgaacttggt
     1261 ggtatgccga cgattccaca acgcatgcaa agcaacggcc tcgcccgatt cggtataggc
     1321 catgcactct ttcgggacga cggtataggc acagcgggta ccggtgaagc ccgccgtctt
     1381 ggagtaactg cggaattcca cggccacttc acgcgcccct tccacctcat agatcgagtg
     1441 cggaagggcg tcatcggtga taaaggcttc ataggccgca tcaaacagaa tcagcgcctt
     1501 gttggccttg gcataatcca cccactgctt cagctgcgcc ttggtagccg ttacgccggt
     1561 cgggttgttg ggaaagcaga gatagatcaa atcaacgggc tgcgttggca acgccggaat
     1621 aaatccgttc tccctgtttc cgtccaggta gaacaggcca tcataacgcc cggtctcctt
     1681 gttccattgg ccggcgcgac cagccatgac attggtatcg acatagacgg gatagacggg
     1741 atcggtcacg gccaccttgc agtcaagcga gaatatttcc tggaaattgc cggtgtcgca
     1801 tttggcgcca tcgctgacaa aaacctcatc cggctgaaca ttggcgccgc gggcctggaa
     1861 atcgaatttg gcgatcgcct cgcgcaaaaa gtcatagccc tgctccggcc catagccacg
     1921 gaacgtgcca tcaccggcca tctcgtcggt ggcggcatgc agtgccttga tgcaggcatc
     1981 gggcaaggcc cgggtcacat ccccaatacc cagtttgatc aatttcttgc cgggattggc
     2041 cttctgaaag gcggcgaccc gcttggcaat atcagagaac aggtaggagg cctgcaattt
     2101 ccggtaattc tcgttaatcc tgatcattca acaccccaaa catacagggc cagaacaatc
     2161 tggccactat tcataataac aacgggataa aactacatgg cgcggtacaa cacaaagctg
     2221 tatcgaacca gttgagcggt catgtcctgc aataggccaa ggatttccgc cgtagtttga
     2281 gacgcgtagt ataagacatg ggctcgcggc tggccaagtc gcatggcggc ggggatggag
     2341 acaaaaaacg gggaccgcag tccccgtttg tactcactga atcagtgatg tgctgcctta
     2401 gtcttcgtac ttgaaagaca tcgaaacgcg ggcgcgatag gccaccacct tgccgttctc
     2461 gatcttcatg tccagtttcg tcacttccgc gacacgcagg tcgcgcaagg tcttggcggc
     2521 cgatgcaatg gccgaactcg cggcatcttc ccaggaaaca ggactggaac ctaccacctc
     2581 gacgatcttg tagacgggat tatcgttctt ggatttcttt gccatggttg cctccggaaa
     2641 attgatttgt gccgagtttt gacctcgact ggctccatca tagcccattc gaataggctg
     2701 ccggtggccg gctctgcagg gccgtgcctc agccgccggc gatgatcggc accaggcgca
     2761 cctggtcacc gctcttgagc gctcggccga gttccccgga cagcaccatt tcgccattca
     2821 ccgtgatgtt ccacgaggga tcgtcgagc
//
LOCUS       DAORVN010000231         2831 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_32594_length_2831_cov_2.894452, whole genome shotgun sequence.
ACCESSION   DAORVN010000231 DAORVN010000000
VERSION     DAORVN010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2831)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2831)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2831
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32594_length_2831_cov_2.894452"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="PKV23_05585"
     CDS             complement(<1..219)
                     /locus_tag="PKV23_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011582427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HQX84941.1"
                     /translation="MKRNILTLMAGALLLGMSSMSAMAAGKTIAVSWKTFQEERWKTD
                     EAAIKAVVEAAGNTYISTDAQGSAQKQAA"
     gene            429..1688
                     /locus_tag="PKV23_05590"
     CDS             429..1688
                     /locus_tag="PKV23_05590"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HQX84942.1"
                     /translation="MLKKANSDLVRRQNRGLFLETLRQHGPLARITLGHKTGLSPASI
                     TSISAQLIDEGLVYSVGEDEAAAAQLRRGRPLTRLALNPRATNVLAVSISVDSIELVF
                     ADFSGATHAPVKLRMDTYDIPKEEFGPRVAHEIKANARKLGIELISISRIGIAVQGVA
                     DTITGEISWSPAFRARDIPVVKPLERILGIPCSIGNNANMIAEALIAADRKKYSGNTA
                     VVFLGHGVGLGLILNGVVYGGANGRAGEFGHMNHLPDGPLCRCGRHGCLEAFSADYGI
                     VRMANSGQTHDFDVHAAVRDEDMRGLEQAAEAGDAIAINAFNRAGEVLGFGIARLIAL
                     INPQQVVLAGPGTRAYGLMKDSIWEALRRGLVHDLRKYVEFEVVPYGKDMITTGTLVE
                     TLRHLDRDVFATGQKSAIVEFAGRKIA"
     gene            1685..>2831
                     /locus_tag="PKV23_05595"
     CDS             1685..>2831
                     /locus_tag="PKV23_05595"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007600277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HQX84943.1"
                     /translation="MRKLLSSAVFALMCIAALGTTPSWASASRPDEVLRTWNQLVLEL
                     VRHTPTYSPPVASRSFAYLGVTAFEAVASGSPEMQSLAGQLNGLTPAPQREAGKTYDE
                     AVVLQAALAFAAQNFFENTGPTGQRALASLEAKLRTKATAGLPADVAVRSEDYGRAVA
                     KHILAWSQDDGGAVIENMGFPLQYELTKGAANWVPTGLAAQQQTPLLPNWGKNRTFAM
                     PSGATCDLPPPPSYSEDKTSEFYKQALEVFETQQNISTEQRAIARFWADDAMLSPTPP
                     GHWIAIALQIFERDNVALDKSVDVLARLGIAEADSFIGCWNSKFQYDLLRPVTYIRRT
                     TDPKWESLLNTPPFPEYPSGHSTQSGAAAVVMTHMFGENFAFKDATHE"
BASE COUNT          605 a          817 c          858 g          551 t
ORIGIN      
        1 tgcggcctgc ttctgagccg agccctgggc atcggtcgaa atgtaagtgt tgccggcggc
       61 ctcaaccacg gccttgatcg cggcttcgtc ggttttccaa cgttcttcct ggaaggtctt
      121 ccaggacact gcaatggttt ttccagcagc catggctgac atggaagaca tgccgagcag
      181 cagggctccg gccatcaaag tcaagatgtt gcgcttcatt ccttaagtcc tcctcatcac
      241 caggccatcc gcgggacgct cccattattt tccagttgtc ctggaactcg caaaggctgg
      301 tcgatggacg attattgatt tattttagcg tccaaaaaaa tgttgcatca agtcgcattt
      361 ggagtcaagc tcagaaaata aatatgacaa ttcagcagta aatgctggag aaattcagtg
      421 ggaacacgat gctcaagaaa gcgaacagcg atcttgtaag acggcagaat cgaggactat
      481 ttcttgaaac cctgcggcag catgggccgc ttgcaagaat tactttgggc cacaagacgg
      541 gcttgtcgcc tgcctcgatc acctccatca gcgcgcaact tattgacgag ggactggtgt
      601 attccgtcgg cgaggatgag gccgccgcag cgcagctcag acgcggacgg ccgctcacgc
      661 gactggcgct caacccacgg gccaccaatg tgctggccgt ttcaatttcg gtcgacagca
      721 ttgaactggt gttcgcggat ttcagcgggg ccacgcatgc gccggtcaag ctgcggatgg
      781 acacctacga cattccaaaa gaggaattcg gaccgcgcgt ggctcacgag atcaaggcca
      841 atgccagaaa gctggggatt gagctgatct caatctcgcg catcgggatt gcggtgcagg
      901 gggtggccga cacgattacg ggagagattt cctggagccc cgccttccgg gcgcgcgaca
      961 ttccggtggt caagccgctt gagcggatct tgggcattcc gtgcagcatc ggcaacaatg
     1021 ccaacatgat cgccgaggcg ctgatcgcgg cggaccgcaa gaaatacagc ggcaataccg
     1081 cggtggtttt cctgggtcac ggcgtggggc tgggcctcat cctcaatggc gtggtctatg
     1141 gcggggccaa tggccgggcg ggagagttcg gccacatgaa ccatcttccc gatggcccgc
     1201 tatgccgctg cgggcgacat ggctgcctcg aagccttcag cgctgattac ggcatcgtac
     1261 gcatggccaa ttccggccaa acccacgact tcgatgtcca tgcggcggtg cgcgacgagg
     1321 acatgcgggg cctcgaacag gcagcggaag ccggcgatgc cattgccatc aatgccttta
     1381 accgggccgg tgaggtgttg ggttttggaa tcgcgcggct cattgcgctg atcaatccgc
     1441 agcaggttgt gctggcggga cccggcacgc gcgcctacgg cctgatgaag gacagcatct
     1501 gggaggcgct gcgccgcggg ctggtgcatg acctgcgaaa gtatgtcgaa ttcgaagtcg
     1561 tgccctatgg caaggacatg atcacaaccg gaaccttagt ggaaaccctg cgccatctcg
     1621 accgcgacgt gtttgcgacg ggccagaaaa gcgcaatagt ggaatttgcc gggaggaaaa
     1681 tcgcatgaga aaactgttgt cctccgccgt gtttgctttg atgtgcatcg cggcgctggg
     1741 cactacgcca tcatgggcaa gcgcaagccg gcccgacgag gtgttgcgca cgtggaacca
     1801 actggttctg gagttggtgc gccacacgcc aacttattcg ccgcccgtgg ccagccgttc
     1861 ctttgcgtac ctcggcgtga ccgcatttga agcggtggcg agcggctcgc ccgagatgca
     1921 atcgctggca ggccagttga acggattgac gcctgcgccg caacgggaag cgggcaaaac
     1981 ctatgacgaa gccgtagtgc ttcaggctgc cctggctttt gccgcacaga atttctttga
     2041 gaacacgggg cccaccggcc agcgggccct tgcctcactt gaggcaaaat tgcgcacgaa
     2101 ggctacggcc ggattgcccg ctgatgtggc agtgcgcagc gaagactacg gccgcgcggt
     2161 cgcaaaacat attctggcat ggtcgcaaga tgatggcggg gcagtcatcg aaaacatggg
     2221 cttccccctg caatacgagt tgaccaaggg cgcggccaat tgggtgccga ccggcctggc
     2281 ggcacagcag caaacaccgc ttctgccaaa ctggggcaag aaccgcacgt ttgccatgcc
     2341 gagtggcgcg acttgtgatt tgccaccccc gccctcctac agcgaagaca agacgtccga
     2401 gttctacaag caggcccttg aggtctttga gacacaacag aacataagca cggaacaacg
     2461 ggccatagcg cggttctggg ctgacgatgc gatgctgtcg ccgactccgc ccggacactg
     2521 gattgcaatt gccctgcaga ttttcgaacg cgataacgtg gcgctggata agagcgttga
     2581 tgtgctggcg cggcttggca tcgcggaagc cgactcattc attggctgct ggaacagcaa
     2641 gtttcagtac gacctgttgc ggcccgtgac ctatatccgc cgcaccacgg atccgaaatg
     2701 ggaatcgctg ctgaacacgc cgccatttcc cgaatacccc agcggccaca gcacacaatc
     2761 aggtgccgcc gcggtggtga tgacccatat gttcggcgaa aacttcgcct tcaaggacgc
     2821 cacgcacgaa c
//
LOCUS       DAORVN010000232         2829 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_32633_length_2829_cov_2.838861, whole genome shotgun sequence.
ACCESSION   DAORVN010000232 DAORVN010000000
VERSION     DAORVN010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2829
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32633_length_2829_cov_2.838861"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..703)
                     /locus_tag="PKV23_05600"
     CDS             complement(<1..703)
                     /locus_tag="PKV23_05600"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HQX84944.1"
                     /translation="MKPASNRFNAKDLWQKDHDHFLHPWTHFDSFKKEGSLLIVEGEG
                     AHITDVNGKRYLDGLGGMWCVNVGYGRKEMAEAMAEQALLLPYANAFVDMSNAPAAEL
                     AAKLAQLAPGSLNHVAFSTSGSCSNDTAVRLAHFYHARRGEPSRKHIISRHNSYHGSS
                     YLTISIGNRDGDRTPNFHYLSDFIHHLSAPYPYRRPDGMTEDQFTDFLVEEFKAKILE
                     VGAKNIAAFIAEPIQG"
     gene            complement(755..2053)
                     /locus_tag="PKV23_05605"
     CDS             complement(755..2053)
                     /locus_tag="PKV23_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018099655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HQX84945.1"
                     /translation="MTTIDEDLAEAMANFVAKRPKTAALHERAKAVMPGGNTRTVLYS
                     APFPIRAERAEGCTIWDVDGHSYLDLVGEYSAGIYGHSNPRIHKAVNDALAQGLNMGA
                     HHTREVIFAEAVTSRFDLDLVRFTNSGTEANMMALAAARCFTGRKKIMPMEGGYHGGT
                     LYFSHGASPVNAPFDCVLARFNDIEKTRALIVQHADDLAAVILEPMLGGGGCLPAAPE
                     FLAMLREETARRGIVLIFDEVMSSRLHPKGLSAKYGIQPDIKTLGKYIGGGMSFGAFG
                     GSHAIMEQFDPSRPNALPHAGTFNNNTLTMTAGHVGLTEIFTAEACVALNERGEELRR
                     AINDLCMGYQAAFQATGVGSMITLHPIAGSIVKPEDADRADKRLKRLLYLYLLDQGIY
                     IAERGFMALSLVVSAVDRQRLLDALESFIKRYRHLLVASA"
     gene            complement(2313..2525)
                     /locus_tag="PKV23_05610"
     CDS             complement(2313..2525)
                     /locus_tag="PKV23_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006022198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX84946.1"
                     /translation="MRTLIGMMLGCLLTIGVVYVHDSMATSTVANETAAGTSRAIVNW
                     DVAKSEWVEVRENVRMAWLKLKANVS"
     gene            complement(2583..>2829)
                     /locus_tag="PKV23_05615"
     CDS             complement(2583..>2829)
                     /locus_tag="PKV23_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011665360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQX84947.1"
                     /translation="LDEPTAALDARSEFEVFKRFKELSEGRTAILISHRFSSVRMADR
                     ILVLADGKVEAAGTHDELVAQSGRYAELFELQAAGYR"
BASE COUNT          593 a          858 c          794 g          584 t
ORIGIN      
        1 aaccctggat gggctccgca ataaaggcgg caatgttctt ggcgcccact tccagaatct
       61 tcgccttgaa ctcctcgacc aggaaatccg tgaactggtc ttccgtcatc ccgtcgggcc
      121 ggcggtaggg atagggcgct gagaggtgat ggatgaaatc cgagagataa tggaaattgg
      181 gtgtccggtc gccatcacgg ttgccgatgg aaatcgtcag atagctcgaa ccgtggtagg
      241 aattatgccg cgagatgatg tgcttgcgtg agggctcgcc gcgccgcgcg tgatagaaat
      301 gggccaatcg gaccgctgta tcatttgaac aggaaccgga cgtcgaaaac gccacgtggt
      361 tgagcgaccc cggcgccagt tgcgccagtt tggctgcaag ttcggcagcc ggtgcgttgc
      421 tcatgtccac aaaggcattg gcatagggca gcagcagcgc ctgttccgcc atggcttcgg
      481 ccatttcctt gcggccataa cccacgttca cgcaccacat gccgccaagc ccatcgaggt
      541 aacgcttgcc gttcacatcg gtgatgtgcg cgccttcacc ctccacaatg agcagcgagc
      601 cttccttctt gaaactgtca aaatgcgtcc agggatggag gaagtgatca tggtccttct
      661 gccacaggtc tttcgcgttg aagcgattgc tggccggttt caaaatgaag tctcccctta
      721 ttgaatgtgc gttcaataca tattagcccg ggggtcaggc cgaagccacc agcaaatggc
      781 gatagcgctt gatgaagctt tcaagggcgt ccaaaagccg ctgcctgtcg accgcactga
      841 ccaccaggga aagcgccatg aagccccgct ctgcaatata gatgccctga tcaagcaggt
      901 agagatagag gagccttttc aaacgcttgt ctgccctatc cgcatcctcc ggtttaacga
      961 tggagccggc tatgggatgc agcgtgatca tcgaccccac ccccgttgcc tgaaacgcgg
     1021 cctggtagcc catgcacagg tcattgatgg cgcggcgcaa ttcctcgccc cgttcattga
     1081 gtgcgacaca ggcttccgcc gtgaaaatct ccgttagccc cacatgtccc gccgtcatgg
     1141 tcagggtatt gttgttgaag gttcccgcgt gcggcagcgc attgggccgt gagggatcaa
     1201 actgttccat gatggcgtgg ctgccgccga aggccccgaa gctcataccg ccgccgatgt
     1261 atttgccgag ggtcttgatg tcgggctgaa tgccgtattt ggcggaaagt cccttgggat
     1321 gcagacggga cgacatcact tcatcaaaaa tcagcacaat gccgcgcctg gccgtctctt
     1381 cccgcagcat cgccaggaac tccggtgctg ccggaaggca cccgcctccc cccagcatgg
     1441 gctcaaggat gaccgccgcc agatcatccg catgctgaac aataagcgcc cgggtctttt
     1501 caatgtcatt gaagcgcgcc agcacacagt caaacggcgc attcaccggc gaggcgccat
     1561 ggctgaaata gagcgtgccg ccatgatagc cgccctccat cggcatgatt ttcttgcggc
     1621 ccgtgaaaca ccgcgccgcc gccagcgcca tcatgttggc ttccgtgccg gaattggtga
     1681 agcgcaccag gtcaagatca aagcggctag tcacggcttc cgcaaagatc acctcgcggg
     1741 tgtgatgcgc gcccatattg aggccttgcg ccaaggcatc attgaccgcc ttgtggatgc
     1801 gcgggttgga gtgcccgtaa atcccggctg aatattcgcc caccagatca agataggaat
     1861 gcccgtccac atcccagatg gtgcacccct cggcccgctc ggcccggatg ggaaacggcg
     1921 ccgaataaag aaccgtgcgc gtattgccgc cgggcatcac ggccttcgcc cgttcgtgga
     1981 gtgccgccgt ttttgggcgc ttggccacaa aattcgccat tgcctcggcg aggtcttcat
     2041 caatcgtcgt cacggctcgg gctccaccac aagttcaggt tttggatcac accttatcga
     2101 aagcgtgggc cgtttgcacc tgttcaagcc tgcgcttcga tcagcgcagc cacgcgtttc
     2161 tggaggtccc ggaggtgacc gccatggatg ccatgggtct cgagcgacag caccgtttgg
     2221 ccggttccat ggcctcctga acgacatcag caaggaaaag gtcatggcgg acatcaagga
     2281 atggatagaa aagcgccttt aggtgttgcc gcctagctga cgttggcttt cagtttcagc
     2341 caggccatgc gcacgttctc cctgacttcc acccactcgc tcttggcaac atcccagttg
     2401 acgatggccc gcgaggtgcc ggcagcagtc tcgttggcaa ccgtcgaagt cgccatggaa
     2461 tcatggacat aaacgacgcc aatagtcagc aggcagccga gcatcattcc aataagggtt
     2521 cgcatggtgt tcgtcctcct taagccacaa cgccttcagg cccggtaagt tccaacatca
     2581 attcagcggt agcccgccgc ctgcaattca aatagctcgg catagcgccc cgattgcgcc
     2641 accagttcat catgggttcc ggccgcttcc acttttccat cggccagaac gagaatgcgg
     2701 tccgccatcc ggacgctgga aaagcggtgc gaaatgagaa tggctgtcct gccttcgctc
     2761 agctccttga aacgcttgaa cacttcaaat tccgagcgcg catccaacgc tgcagtcggt
     2821 tcgtcaaga
//
LOCUS       DAORVN010000233         2820 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_32810_length_2820_cov_2.960579, whole genome shotgun sequence.
ACCESSION   DAORVN010000233 DAORVN010000000
VERSION     DAORVN010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2820
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32810_length_2820_cov_2.960579"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1023)
                     /locus_tag="PKV23_05620"
     CDS             complement(<1..1023)
                     /locus_tag="PKV23_05620"
                     /inference="COORDINATES: protein motif:HMM:NF017021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HQX84948.1"
                     /translation="MKKRLVWMGVALFLGLFAAGIWVFIQPGFAVAEVQDYVMRKTGR
                     TLLVNGGARLEFLPQLSVRLDDVFLSNPEGMDGNFARAASARLPLQLNDLIRRKLKIR
                     HVTLDRPVFNVLIDREGRGNWVTGEKVAQGNSGQSNGGSSAEPLTFRVESGSASFLDE
                     RSGQAFSLEDASARIAIGEDGGLAVQGTAALNKQSATIEAEVNSLQRVSEDGSPFNLT
                     IRAPALTVNFTGRLSTRDGIGLAGAIDATAPDLRQLSKWLGSEIGGNAGLKNFSLAGA
                     LDSKNAAFNLTKASVALDGMVANGDLAVDLTRKMPRVIATFSTDIFSLDPYLPPLPSA
                     ARVQRAG"
     gene            1081..>2820
                     /locus_tag="PKV23_05625"
     CDS             1081..>2820
                     /locus_tag="PKV23_05625"
                     /EC_number="6.2.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018699648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030729 - acetoacetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate--CoA ligase"
                     /protein_id="HQX84949.1"
                     /translation="MPDQPLWTPPEKRKSGSNLRRFMDELERTETDSLQSYADILGFS
                     TAKPEAFWLKLWEFCGVVAEARGKTVLVDGDRMPGGRFFPDARLNYAENLLRRNDDKP
                     ALVFWGEDRIRLNMTWRQLNARVAQTHRALAKAGIKQGDRVCAVVPNHPESIVAFLAV
                     ASLGAIWSSCSPDFGERGILDRFGQIAPKLLFTCDAYFYAGKTHSLAEKIGKVLTQLP
                     SVEHTVVIDYTGQAEAMAAHLPAAKTLDDFRSDETDVPIAFAQMPFDHPLYILYSSGT
                     TGIPKCIVHRAGGILLKHLSEIVLNSDVKPGDRLFYFTTCGWMMWNWLVSGLAAGATL
                     LLYDGSPFHPSEQALFDFADAEDMTIFGTSAKYIDSVRKTGWRARDTHKLANLRTMMS
                     TGSPLSAENFDFVYDAVKPDIHLASISGGTDICGCFVGGNPLGSVWRGEIQGPMLGMA
                     MDVLDDNGRSVKQEKGELVCTRPFPSMPVMFWNDPDGQKYHNAYFARFDNIWCHGDFA
                     EITAHGGLIIHGRSDATLNPGGVRIGTAEIYAQVERIPEVLEALAIGQDWDSDVRVVL
                     FVRLAPGVALDDRL"
BASE COUNT          586 a          866 c          798 g          570 t
ORIGIN      
        1 cccagcgcgt tgcactcgag cggccgaagg aagcggtggg agataagggt cgagagagaa
       61 aatatccgtt gaaaacgtgg cgatgaccct tggcattttt ctggtgagat caaccgccag
      121 atcaccattt gcaaccatgc cgtccagtgc aacactggcc ttggtcagat tgaaagccgc
      181 gttcttcgaa tccagggcgc cggccagtga aaaattcttg aggcccgcat tccccccgat
      241 ctccgaaccc agccatttcg aaagctggcg cagatcgggt gcggtggcgt caattgcgcc
      301 ggcaaggcct attccgtcgc gcgtagacag cctgccagta aaattcaccg tgagcgccgg
      361 ggccctgatc gtgaggttaa acggcgaacc atcctcgctc acccgctgca gggaattcac
      421 ctcggcctca atagtggcag actgcttgtt gagcgccgct gttccctgca ctgcaagccc
      481 gccgtcttcg ccgatggcaa ttcttgccga agcatcttca agcgaaaacg cctggccact
      541 tcgctcgtca agaaaactgg ccgagccgct ttccacccgg aatgtcaggg gctccgcgga
      601 actgccgcca ttggattgcc cgctattgcc ctgggcgact ttctcaccgg tcacccagtt
      661 gccgcgcccc tcccggtcta tcagaacgtt gaacaccggc cggtccaatg tgacgtgccg
      721 gatttttagc ttgcgccgga tgagatcatt gagttgcagg ggcaagcggg cgctcgcagc
      781 ccgggcaaag ttgccatcca tgccttcggg gttggagagg aaaacatcgt cgaggcgcac
      841 gctgagttgg ggcagaaact cgagccgggc gccgccattg accagaagcg tgcggcccgt
      901 cttgcgcatg acatagtcct gcacctcggc aacggcaaaa cccggctgga tgaaaaccca
      961 gatcccggcg gcaaaaagcc cgaggaacaa ggccactccc atccagacaa gtcgcttttt
     1021 cattcttcct cctttggaag ccggatatat tatggaccaa attccaagcg gagataagta
     1081 atgcccgacc agcccctgtg gacgccacca gaaaagagga agtcgggctc gaatctccgc
     1141 cggtttatgg atgaactgga acgcaccgaa actgacagtc tgcaatccta tgcggatatt
     1201 ctgggattct ccacggcaaa gccggaagcc ttctggttaa agctgtggga attctgcggt
     1261 gtggtggcgg aagcccgtgg aaaaaccgtg cttgtcgacg gggatagaat gccgggaggc
     1321 cgctttttcc ccgatgccag gctgaactat gccgaaaacc tcttgcgccg gaacgatgat
     1381 aagcctgccc tggtcttttg gggcgaagac aggatcaggc tgaacatgac ctggcgccag
     1441 ctcaatgcgc gcgtcgccca aacccaccgc gcccttgcca aagctggcat caaacagggc
     1501 gaccgggtct gcgccgtggt gcccaatcat cccgaatcca ttgtggcttt cctggcggtg
     1561 gcctcgctcg gggccatctg gtcatcctgc tcgccggatt tcggcgagcg cggcattctt
     1621 gaccgcttcg gccagatcgc accaaagctc cttttcacct gcgatgccta tttctacgcc
     1681 gggaaaaccc acagcctcgc tgagaagata ggcaaggttc tgacccagct gccaagtgtc
     1741 gaacacaccg tggtgatcga ctacaccggc caggccgaag cgatggcagc acacttgccg
     1801 gcagcgaaaa ccctggacga cttccgctca gatgaaactg atgtgcccat tgccttcgcc
     1861 cagatgccct tcgatcaccc gctctatatt ctttattcct ccggcaccac cggcattccg
     1921 aaatgcatcg tgcaccgcgc cggcggcatt ttgctgaagc acctgtctga gatcgtattg
     1981 aacagcgatg tgaaaccggg cgaccgcctt ttctatttca ccacctgcgg ctggatgatg
     2041 tggaactggc tggtcagcgg cctggccgca ggcgccaccc tgctgctcta tgacggttcg
     2101 ccttttcatc ccagtgaaca ggcactcttt gattttgctg acgcagaaga catgacgatc
     2161 ttcggaacct cggccaaata cattgattcc gtgcgcaaga ctggctggcg cgcccgcgat
     2221 acccacaagc tcgccaacct ccggaccatg atgtccaccg gttcgccgct cagtgccgaa
     2281 aatttcgatt ttgtctatga cgcggtcaaa cccgacatcc acctcgcctc catatcgggc
     2341 ggcaccgata tctgcggctg ctttgtgggc ggcaatccgc tgggaagtgt ctggcgcggc
     2401 gaaatccagg ggccgatgct cggcatggcc atggatgtgc tggatgacaa tggccgatcc
     2461 gtaaagcagg aaaagggcga actggtctgc accagaccgt ttccgtcgat gcctgtcatg
     2521 ttctggaatg accccgatgg ccagaaatat cacaacgcct atttcgcccg ctttgacaat
     2581 atctggtgcc acggcgattt cgccgaaatc accgcccatg gcggcctcat cattcatggc
     2641 cgttctgatg caaccctgaa cccgggcggg gtgcgcatcg gcacggctga aatctatgcc
     2701 caggttgagc gcatccccga agtgctggaa gctctggcca tcggccagga ctgggacagt
     2761 gacgtgcgcg tcgtgctctt tgtgaggctg gcgccgggcg tggccctcga cgacaggctg
//
LOCUS       DAORVN010000234         2814 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_32946_length_2814_cov_2.661472, whole genome shotgun sequence.
ACCESSION   DAORVN010000234 DAORVN010000000
VERSION     DAORVN010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2814
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32946_length_2814_cov_2.661472"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..311
                     /locus_tag="PKV23_05630"
     CDS             <1..311
                     /locus_tag="PKV23_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002966479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HQX84950.1"
                     /translation="ADNDNVRWVYLDIGKFGGLAETMDEAIRYPITTARDGDAKTPCV
                     IAGPTCDSADVLYEKTPYDLPVTLSVGDEVLIEATGAYTTTYASVAFNGFTPLQAYII
                     "
     gene            348..878
                     /locus_tag="PKV23_05635"
     CDS             348..878
                     /locus_tag="PKV23_05635"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HQX84951.1"
                     /translation="MVTIRAEIPADVTAREALLDRAFGRMRNRKTSQRLRDERLPADG
                     LAFTALSKSGRVIGTLRMWNVIAGSAGPALLLGPLAVDGRYQRRGIGRELMTHALNMA
                     KVLGHASVLLVGDEPYYSRYGFTRNLVADLHLPGPVERGRFLGVELAPGALDGAEGLV
                     MASGALAPAVGKTLAA"
     gene            complement(879..1433)
                     /locus_tag="PKV23_05640"
     CDS             complement(879..1433)
                     /locus_tag="PKV23_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018390004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-3-hexuloisomerase"
                     /protein_id="HQX84952.1"
                     /translation="MTNLYKSALAELDVVFDRLNDGDVDKACGMIAAARKTVVFGGGR
                     ERLQIMGFAMRLYHMGLAVAVEGDMTTPPVGKGDLFVVTCGPGEISTALALMGVARAA
                     GSKILFITAQPQSRGAALADFVLHLPAQTMADDQGDKRSSVLPMGSLFEGALFVLFEV
                     MVLKLIASLQIKPEAMRANHTNLE"
     gene            complement(1495..2721)
                     /locus_tag="PKV23_05645"
     CDS             complement(1495..2721)
                     /locus_tag="PKV23_05645"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013897030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HQX84953.1"
                     /translation="MANEKVETLIIGGGQAGLAMSAHLGKRAVPHLIIERHRIAERWR
                     SERWDSLVANGPAWHDRFPSLEFSDIDPNSFAPKDRIVEYFEAIAKQIKAPIRCGVEV
                     KSVERKADGSGFRAETSEGVIEASNVVAATGPFQRPIIPAVVPPDAGIMQVHSNAYRN
                     PGQLPQGAVMVVGAGSSGVQIADELLRAGKRVYLSVGPHDRPPIRYRGRDFCWWLGVL
                     GMWDAKTREPGMEHVTIAVSGAHGGHTVDFRKLAARGITLLGRAGPFKDGVMHCADDL
                     ARNIAQGDAHYLSVLDAADAYVAKNGLDLPEEPEARRIDANPDCVTDPILQLNLAEAG
                     ITAIVWATGFAVDFGWLKADTFDARGIPLHERGVSKVPGLYFLGLPWLSRRASPFIWG
                     VWHDAEYLAGHIAARC"
BASE COUNT          535 a          869 c          877 g          533 t
ORIGIN      
        1 aggccgacaa cgacaatgtg cgctgggtct atctcgacat cggcaagttc ggcggcctgg
       61 ccgaaaccat ggatgaagcc atccgctatc cgatcaccac ggcgcgcgac ggcgacgcca
      121 agacaccttg cgtgatcgcc gggccaacct gcgattcggc tgacgtgctt tatgaaaaga
      181 cgccctatga cctgccggtc acgctgagcg tcggcgatga agtgctgatc gaagccacgg
      241 gagcctacac gacgacctat gcgtcggtgg ctttcaacgg ctttacgccg ctgcaggcct
      301 acatcatcta gttcacctca tcccctgtcc aatcactgga ggctgtcatg gtcaccattc
      361 gcgccgaaat tccggctgac gtcaccgccc gcgaggctct tctcgaccgc gccttcgggc
      421 gcatgcgcaa ccgcaagacg tcacagcgct tgcgcgatga gcgcttgcct gccgatgggc
      481 tggcgttcac cgccctgagc aaatcgggcc gcgtgatcgg cacgcttcgc atgtggaatg
      541 tgattgccgg ctcggcgggg cctgcgcttc tactgggacc gctggcggtg gatggccgct
      601 accagcggcg cggcatcgga cgggagctga tgacccatgc cctcaacatg gcgaaagttc
      661 tgggccacgc gtcagtattg ctggtgggcg acgaacccta ttacagccgt tatggtttca
      721 cgcggaacct ggttgcggac ctgcatctgc ccggcccggt ggagcgcggc cgtttccttg
      781 gcgttgaact tgcgccgggc gcgcttgatg gtgccgaagg cctggtgatg gccagcggtg
      841 cgcttgctcc ggctgtgggc aagacactgg cggcctgact attcgaggtt tgtgtggttg
      901 gcgcgcatcg cttcaggctt gatctgaagc gatgcgatga gcttcagcac catgacttcg
      961 aagagcacga acagggcgcc ctcgaacagc gatcccatgg gcaaaaccga tgagcgcttg
     1021 tcgccctgat cgtccgccat ggtctgggcc ggcaaatgca agacgaagtc cgccagtgcg
     1081 gcgccacggc tttggggttg agccgtgata aagagtatct ttgaacccgc cgccctggcc
     1141 acgcccatga gggccagggc cgttgagatt tcaccgggtc cgcaggtcac aacaaacaaa
     1201 tcgccttttc caacgggcgg ggtcgtcatg tcgccttcga cagcaacggc gaggcccatg
     1261 tgataaagcc gcatggcaaa ccccatgatt tgcagacgct cacggccgcc gccaaatact
     1321 acagttttgc gggccgcggc aatcatcccg caagccttgt cgacatcgcc gtcattcagc
     1381 cggtcgaaga cgacgtcgag ctcagccagc gctgatttgt agagatttgt catattgggg
     1441 tttccttaac tccgatacca aaccttggat gactggccct gaagtgtaaa gggactagca
     1501 gcgcgcggcg atatggcccg ccagatactc ggcgtcgtgc cagacgcccc agatgaaggg
     1561 cgaggcccgg cgcgacagcc agggcaatcc cagaaaatag aggccgggca ccttggatac
     1621 accgcgctcg tgaagcggga tgccgcgcgc atcgaaggtg tcagccttga gccagccaaa
     1681 atcaacggca aagcccgtgg cccagacaat cgcggttatg ccggcctcgg ccaagttaag
     1741 ctggagaatg ggatcggtca cgcagtcggg attggcatca atcctgcggg cttccggttc
     1801 ttccggcagg tcgaggccgt tcttcgccac ataagcatca gcggcgtcca gcacggaaag
     1861 gtaatgggca tcgccttgcg cgatgttgcg cgcgagatca tcggcgcaat gcatcacgcc
     1921 atccttgaac gggccggccc ggcccagcag ggtgatgccg cgcgcggcca gtttgcggaa
     1981 gtccacggtg tggccaccat gggccccgct caccgcgatg gtgacatgtt ccatgcctgg
     2041 ttcgcgggtt ttggcatccc acatgccgag aacccccagc caccagcaga aatcccgccc
     2101 gcggtaacgg atcggcggcc ggtcatgggg gccgaccgaa agataaacgc gctttcccgc
     2161 ccgcaggagt tcatcggcaa tctggactcc agaagacccg gcacccacca ccattacagc
     2221 accttgcggc aactggccgg gattgcggta ggcattggaa tggacctgca tgatcccggc
     2281 atccggcggc acgacggcgg gaatgatcgg gcgctggaac ggcccggttg cggccaccac
     2341 gttgctggcc tcgatcacgc cttcggacgt ttcggcgcgg aagcccgagc catcggcttt
     2401 cctttccacc gacttgacct cgacaccgca gcggatgggg gccttgatct gctttgcgat
     2461 ggcttcgaaa tattcgacga tccggtcctt gggggcaaag ctgttggggt caatgtcgga
     2521 gaattccaga cttgggaagc ggtcatgcca ggccgggccg ttggcgacaa gcgagtccca
     2581 tctttccgag cgccagcgtt cagcaatccg gtgccgctcg ataatgaggt gtggcacggc
     2641 gcgcttgccg agatgcgcgc tcatcgccag gccagcttgg ccgccgccaa tgatgagggt
     2701 ctcgactttt tcatttgcca tggcggcatc cactttcgct gtttacctgg ccgatattga
     2761 ataccagccg cggcgaaaga ctattgtatg cgtggctttc cgtcgaggag actg
//
LOCUS       DAORVN010000235         2812 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_32976_length_2812_cov_3.804135, whole genome shotgun sequence.
ACCESSION   DAORVN010000235 DAORVN010000000
VERSION     DAORVN010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2812)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2812)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2812
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32976_length_2812_cov_3.804135"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /locus_tag="PKV23_05650"
     CDS             <1..293
                     /locus_tag="PKV23_05650"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HQX84954.1"
                     /translation="ALGARTLRQDRLFECNSLRLMAALARDGGCIAFQTLFGIERDVA
                     ERSLVFIPLSDRKLMPSRVTLVHRPGLDRKTAAGAFLELAKQRLPRIFDVRK"
     gene            401..751
                     /gene="uraH"
                     /locus_tag="PKV23_05655"
     CDS             401..751
                     /gene="uraH"
                     /locus_tag="PKV23_05655"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010972230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HQX84955.1"
                     /translation="MGRLTTHVLDMSSGKPAAGLKVELWRIGLRPELVVTVGTNRDGR
                     VEGAVLEGDSFKAGIYELRFHAGAYLRGTGAKMEEPAFLDVIPIRFGIADVNQHYHVP
                     LLLSPYGYSTYRGS"
     gene            754..2223
                     /gene="xdhA"
                     /locus_tag="PKV23_05660"
     CDS             754..2223
                     /gene="xdhA"
                     /locus_tag="PKV23_05660"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011005354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004854 - xanthine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009115 - xanthine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase small subunit"
                     /protein_id="HQX84956.1"
                     /translation="MARQAIRFLRRGQVVELSSLPPTRTLLDYLRLEEKSKGTKEGCN
                     EGDCGACTVALGSLEEGRVVYEPVNACILLLGQIDGKELVTVDDLAAGGKLHPVQKAF
                     VDNHASQCGFCTPGFVMSLFTLYHTGRKPTRQEIVDHIAGNLCRCTGYRPIVDAAVKS
                     CTGKPADAWVAAAGATAVQLAALDDAADIFVGDTSTFFAAPYHEEELTRLYADHPDAT
                     IVAGATDVGLWVTKQLRTLPKIIHIGRTRGLDRITDEGPHISIGATATYSSAEAFLAS
                     IDPDIGEVIRRIGATQVRASGTIGGNIANGSPIGDTPPMLIALGATLHLRWESRERFL
                     PLEDFFVAYGKQDRAAGELVWRVDVPRLKANEAFRAFKLSKRFDQDISAVMGAFKFTL
                     EGRTIQSARIAFGGMAATPKRALIAEAALRGISLDAPKAWEQAISAMAEDYQPIGDMR
                     ASAEYRLASAQALLRKALVEVASGQSAATRLAGLRGEAA"
     gene            2225..>2812
                     /locus_tag="PKV23_05665"
     CDS             2225..>2812
                     /locus_tag="PKV23_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HQX84957.1"
                     /translation="MNVRARILETDTVGLALAHDSAALHVQGTATYIDDMREPEGTLH
                     VEPGYAPNDARGKVTSLNLGAVRAFPGVVAVLTAKDIPGVNDCSPGPGDDPIFAEGEI
                     EFHGQVIFAVVAETREIARRAARLAKIEVAAMTPAVTVEDALAMATRDVVPEYAFTYG
                     DVKKALKAAPHVISESFHVGGQEHFYIEGQVALAMP"
BASE COUNT          534 a          787 c          919 g          572 t
ORIGIN      
        1 gggcgctggg ggcgcggacg ctgcggcagg ataggctgtt tgaatgcaat tccctgcgcc
       61 tgatggcggc cctggcgcgg gatggcggct gcatcgcgtt tcaaacgctc ttcggcatcg
      121 agcgggatgt ggcggaaagg agcctggtgt tcatccccct gtccgaccgc aagctcatgc
      181 cgagccgggt cacccttgtg caccgcccgg ggctagaccg gaaaaccgcg gcgggggcgt
      241 ttctcgagct ggcgaagcag cggctgccgc gaatttttga tgtgcgaaaa tagttattgt
      301 tgatgtgctt tttttatgcc ttttagaaca ggcagaatct gttaaaaatt ccaaatccgc
      361 cacgaagagc ggatttcaac gaaaattttt ggggaaacgc atgggtcgtc ttacgacgca
      421 cgttcttgat atgagttcag gcaagccggc ggcgggcctg aaagtggaac tatggcgcat
      481 cggcttgcgg ccggaacttg ttgtcacggt cggcaccaac cgggatggca gggtggaggg
      541 cgccgttctc gaaggcgaca gtttcaaggc cggcatctat gagctccggt tccatgcggg
      601 cgcctatttg cgcggcacgg gcgcgaagat ggaagagccg gcattcctgg acgttatccc
      661 catccggttc ggcattgccg acgtgaacca gcattatcac gtgccgctgc tgctctcgcc
      721 ctatggctat tcgacctaca ggggcagcta ggcatggcgc gccaggcaat ccgctttttg
      781 cgccgggggc aggtggtgga gctgtcctcc cttccgccga cgcgcacact gctggattat
      841 ctgcgccttg aggaaaaatc gaagggtacc aaggagggct gcaacgaggg cgattgcggc
      901 gcctgcaccg tggcgctggg ttcgcttgaa gaaggccggg tcgtctacga gccggtcaat
      961 gcctgcattc ttctccttgg ccagattgac ggcaaggaac tggtcacggt tgacgatctg
     1021 gccgcgggtg gaaaactgca tcccgtgcag aaggcctttg tcgacaatca cgcctcgcaa
     1081 tgcgggttct gcacgccggg tttcgtcatg tccctgttca cgctctatca cacgggcagg
     1141 aagccgacgc ggcaggagat cgtcgatcat atcgcaggca atctctgccg ctgcacgggc
     1201 tatcgcccga ttgtggatgc ggcggtgaaa tcctgcacgg gcaaaccagc cgatgcctgg
     1261 gtggcagcgg ccggggcaac agccgttcaa ttggcagcgc ttgatgacgc tgcagatatt
     1321 tttgtgggcg ataccagtac tttctttgca gcaccttacc atgaggagga actcaccagg
     1381 ctctatgcag accatcccga tgccacgatt gttgcgggcg caaccgacgt ggggctttgg
     1441 gttaccaagc agcttcgaac cctgccgaag atcatccata tcggccggac cagggggctt
     1501 gatagaatca ccgatgaagg cccgcatatt tccattggcg caacggcgac ctattccagt
     1561 gccgaagcgt ttctcgcctc gattgatccc gatatcggcg aagtgatccg ccgcatcggg
     1621 gccacgcagg tgcgggcctc gggaacgatt ggcggcaata tcgccaatgg ttcgcccatt
     1681 ggtgacacac caccgatgct gattgccctg ggagcgacgc tgcatctccg ctgggaaagc
     1741 agagagaggt ttctgccgct tgaggatttt tttgtggcct atggcaagca ggaccgcgct
     1801 gccggtgaac tggtctggcg agtcgatgtt cccaggctca aagccaatga agcgttccgg
     1861 gccttcaagc tcagcaagcg tttcgaccag gatatttcgg ctgtcatggg tgccttcaag
     1921 ttcacactgg agggccgcac gatccaatcc gcgcggatcg cttttggcgg catggcggca
     1981 acgcccaagc gtgcgctgat agcagaagcg gcgctgcgcg ggatttcgct cgatgcgcca
     2041 aaggcctggg agcaggcgat ttcggcgatg gcggaggatt accagcccat cggtgacatg
     2101 cgggcttcgg cggaataccg gctggcatcg gctcaggccc tgctgcgcaa ggccctggtg
     2161 gaagtcgctt ccggtcaatc ggcggccacg cgccttgccg ggcttcgcgg ggaggccgcg
     2221 tgagatgaat gtgcgggccc gcatcctcga aacagatacg gttggcctgg cgctcgccca
     2281 tgacagcgcg gcgctgcatg tccagggaac cgcaacttac atcgatgaca tgcgcgagcc
     2341 cgagggcacg ctgcatgtgg agccgggcta tgcgcccaat gatgcgcggg gaaaggttac
     2401 cagcctcaat cttggtgcag tccgcgcctt tcccggcgtt gtcgcggtgc tgacggcgaa
     2461 ggatattccc ggcgtcaatg attgcagccc cggtcctggc gatgatccca tttttgctga
     2521 aggagaaatt gaatttcatg gccaggtgat ttttgcggtg gttgcggaaa cacgcgagat
     2581 tgcgcggcgc gcggcgcggc tggcgaagat tgaagtggcg gccatgaccc cggcggtgac
     2641 ggtggaagac gcgctggcca tggctacccg ggatgtggtg ccggaatatg ccttcactta
     2701 tggcgacgta aaaaaagcac tcaaggcggc accacacgtg atttccgaga gcttccacgt
     2761 gggcgggcag gagcatttct atatcgaagg gcaagtggcg ctcgccatgc ct
//
LOCUS       DAORVN010000236         2811 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_33013_length_2811_cov_2.846517, whole genome shotgun sequence.
ACCESSION   DAORVN010000236 DAORVN010000000
VERSION     DAORVN010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2811
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33013_length_2811_cov_2.846517"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..647
                     /locus_tag="PKV23_05670"
     CDS             <1..647
                     /locus_tag="PKV23_05670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX84958.1"
                     /translation="DEARPAGSVEGGGFAIVPDMMSLVGCSGEEFEGILRSLGFRMQK
                     RKIKRPAAAPPVVAAESPPAETEASADPAAMAVEPEAATTVSEPELVEVPADAAAPPE
                     AEALLTPDVAAPPSAGPLLEEVEIEVWWPKDTGPFRHRPEAKPQQRPQPKQRPQPKPV
                     DQKERPPRSNRKPHAKERNEHREEPRRRPEKPMDPNSPFAILETLKAQMMGKKN"
     gene            656..931
                     /locus_tag="PKV23_05675"
     CDS             656..931
                     /locus_tag="PKV23_05675"
                     /inference="COORDINATES: protein motif:HMM:NF013633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HQX84959.1"
                     /translation="MDKWLWFARVVKQRPKAAELVETGHVRLNRMKVTKPGHVLRPGD
                     VLTIAVGGHVRVLRVKDLGIKRGPFAEACQLYEDLTPGPSRDEKDRA"
     gene            983..1321
                     /locus_tag="PKV23_05680"
     CDS             983..1321
                     /locus_tag="PKV23_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008528408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HQX84960.1"
                     /translation="MTYIVNDKCIKCKFMDCVEVCPVDCFYEGENMLVIHPDECIDCG
                     VCEPECPVDAIKPDTEPGLEKWLKINAEYAPKWPNISIKRDPPPDAKEFEGVEGKFEA
                     YFSEKPGEGD"
     gene            1537..2439
                     /locus_tag="PKV23_05685"
     CDS             1537..2439
                     /locus_tag="PKV23_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011082988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HQX84961.1"
                     /translation="MKKTVKKAALAKKAAHTRKSVAKAKNATKAVAAKAKSKALKAKP
                     KAKVASAAKPAAKPVPKKTILTKVTGHLKNAKPVHAAPAPVAKPVAVAVKPVAAPVAA
                     VAKPAVAAAPAAPKKLSFKIGELVVYPAHGVGKITAIEEQEIAGSKLELYIVDFEREK
                     LRLKVPTNRAEQKGMRRLADRGLIEHAMKVIRGRARIKRTMWSRRAQEYDAKINSGDL
                     IAVAEVVRDLYRSDRQPEQSYSERQLFEQALLRMARELAAVRKVDDDQSVKELSEFLA
                     KNAKRAQAAGEKAEEEAAAAEEAA"
     gene            2529..2605
                     /locus_tag="PKV23_05690"
     tRNA            2529..2605
                     /locus_tag="PKV23_05690"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2563..2565,aa:Arg,seq:cct)
BASE COUNT          663 a          862 c          792 g          494 t
ORIGIN      
        1 ccgatgaggc gcgccctgcc ggctccgtcg aaggcggcgg ctttgccatt gtgcctgaca
       61 tgatgtcact tgtcggctgc tccggcgaag aatttgaggg catcctgcgc agcctcggct
      121 ttcgcatgca gaaacggaaa atcaaacgtc cggcggccgc gccgcccgtc gtggctgccg
      181 aaagcccccc tgctgaaaca gaagcatccg ccgatccggc agcaatggca gtggagcctg
      241 aagccgccac aacggtctct gaacctgaac tcgtagaagt gccagcggac gctgcggccc
      301 cgccagaggc tgaggccctg ctgacgccgg acgttgccgc tccgccgtct gccgggcccc
      361 tcctagaaga ggttgaaatc gaggtctggt ggccaaagga tacgggcccc ttccgccacc
      421 gccccgaggc caaaccccag caacggcctc aacccaagca acggcctcaa cccaagcccg
      481 tggaccagaa agagcggccg ccgaggtcca accgcaagcc ccacgccaag gaacgcaatg
      541 agcaccgcga ggagcccagg cgccggcctg aaaagcccat ggaccccaat tcgccctttg
      601 ccatcctcga gaccctcaag gcgcaaatga tgggtaagaa gaactgacgc aacgtctgga
      661 caagtggctt tggtttgccc gtgtcgtcaa gcagcgcccc aaggccgctg aactggtgga
      721 aacgggccat gtgcgcctga accgcatgaa ggtcacaaag ccgggccatg ttctgcgccc
      781 gggagatgtc ctcaccattg ccgtgggcgg ccatgtccgc gtcttgcggg tgaaggattt
      841 gggcatcaaa cgcggcccct ttgccgaggc atgtcagcta tacgaagacc tcacccctgg
      901 cccttcacgc gacgaaaaag accgtgcata aggcgggaaa cattgctaag catcgttcgt
      961 tataaagaac aactggaccg acatgaccta catcgtcaat gataaatgca tcaaatgcaa
     1021 gtttatggac tgcgtcgaag tctgccccgt ggactgcttc tacgagggcg aaaacatgct
     1081 ggtcatccac cccgatgaat gcatcgattg cggcgtctgc gagccggagt gcccggttga
     1141 cgcgattaag cctgataccg agcccggcct cgaaaaatgg ctgaaaatta atgcggaata
     1201 tgcgccgaaa tggcctaata tatcgatcaa gcgcgatccg ccgcccgatg ccaaggaatt
     1261 tgaaggtgtc gagggaaaat tcgaggccta tttctcggaa aagcccggcg aaggcgattg
     1321 aattcgttaa ccatcctgtg aataacgcag ggctccgatg ccgagataga ccgtacacgg
     1381 atatatcgag ctttaatttt ccttaaaatc gtgctatagt catttttaat gtaaacaacg
     1441 cccctgcaag tcatcagacc cgagccccta aagctcggtt attttttcgc ccgttttttg
     1501 gcgaatccag ggtccagacg aaggcgaagg acaagaatga aaaagaccgt caaaaaggca
     1561 gctttggcaa agaaggcagc tcacacgaga aaatcggttg cgaaggcaaa aaatgcaacc
     1621 aaggctgtgg ccgcgaaggc caagtccaaa gctttgaaag ccaagcccaa ggcgaaggtg
     1681 gcttccgctg caaagcctgc ggccaaaccc gtccccaaga aaacgattct gaccaaggtg
     1741 accgggcatc tgaagaacgc caagccggtc cacgcggctc ctgcgcccgt ggcaaagcct
     1801 gtagccgtgg ccgtaaaacc cgttgccgca cctgttgcag ccgtggccaa gcccgccgta
     1861 gccgccgcac ctgcagcgcc caagaagctt tccttcaaga ttggcgaact cgtggtttac
     1921 cccgcccatg gcgttggcaa gatcaccgcc attgaggagc aggaaatcgc cggctcgaag
     1981 cttgaactct acatcgtgga tttcgagcgc gaaaagctgc gcctcaaggt gccgaccaac
     2041 cgtgccgagc agaagggcat gcgccgcctg gctgaccgtg gcctgatcga gcatgccatg
     2101 aaggttatcc gtggccgggc ccgcatcaag cgcaccatgt ggtccaggcg cgcccaggaa
     2161 tatgacgcga agatcaattc cggcgatctc atcgccgtgg ccgaagtcgt gcgcgatctc
     2221 taccgctccg accgccagcc cgagcaatcc tattccgagc gccagctgtt tgagcaggcc
     2281 ctgctccgca tggcccgcga gctggccgcc gtccgcaagg tggatgacga tcagtcggtg
     2341 aaggagcttt ccgaattcct cgccaagaac gccaagcgcg cccaggccgc cggcgagaag
     2401 gcggaagaag aagccgctgc cgccgaagag gccgcataag agttttccaa gacctcgacg
     2461 gacaggcccg gaccaaaaat ccgggccttt tctttttcac ggattccaaa ctataaggag
     2521 gcttatgcgg tcccgtagct cagttggata gagcatcagt ttcctaaact gagggccact
     2581 ggttcgaatc cagtcggggc cgccagtcat tccagatcac gcaaaagatc aggcgtagcc
     2641 ctcagcattg ttaaagtttc cagcccttgc ccttgctgtt gtcattaccc ctcatgagtg
     2701 gcgcaatcga gcacctctgc gggaacgccg cctttgtcag cggaaaaacc gaaatcttgc
     2761 aaatcacggc ctgcgcgcaa gtaacgttgc gccgagcccg ctgccctggc g
//
LOCUS       DAORVN010000237         2804 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_33158_length_2804_cov_3.370316, whole genome shotgun sequence.
ACCESSION   DAORVN010000237 DAORVN010000000
VERSION     DAORVN010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2804)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2804)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2804
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33158_length_2804_cov_3.370316"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            299..2389
                     /locus_tag="PKV23_05695"
     CDS             299..2389
                     /locus_tag="PKV23_05695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HQX84962.1"
                     /translation="MNVAVKSAAEVPAFSGWDELDRKAPSWRIEREEVQGDAIAPFFP
                     DQNGGVYEQLLFNFPFMMHAMDGSGRLNSVNRQWCKTLGYMPSDVIGKSFNELLTPQS
                     RSHLITTIYPKYLQTGLCRGEEMFVHRKDGTLATVSLSMNAYRGDKGRIERSICLMHD
                     VTDQKIAEIASVRSDQRFRGAFAAAAHGMALVSPTGQIELSNAAFGAFLGREDIDRST
                     LTFDETLHRDDRGQFLNGMRQLLSGEVPSLKQELRYTTGTGGVVHGSTSVSMVRSEKA
                     EIEQLVVQIVDVSERKLTTQRLHQAQKMEAIGQLTGGLAHDFNNLLTIIMGNLQLLEG
                     KLAGDEKAVKRASEALDAARKGSDLTRQLLAFARKQDLEPREVKINDLVTGMEALISR
                     TLGENVELKVDVAAGSPQVLIDPSQFESSILNLAINARDAMPNGGLLTIETQLVHLDR
                     EYADRHPEVVPGDHVLVAISDTGIGMSPELLEKVFHPFFTTKENGKGTGLGLSMVYGF
                     IKQSGGHISIYSEVGHGTSVKMYLPRKMLAGETADPPAAENYSADTAPAIAAPAETEV
                     PKKAPRPQKILVVEDQEAVRAVACGFLMDFGYDVVEAEDGFQALSRLQEDPEIDLMFS
                     DVVMPGGMNGFDLAQAASGMRPELKIVHTSGYPKGAMVHQDEPRFRQGFIIMKPYRRE
                     ELQKIIRDAFEKQG"
     gene            2460..>2804
                     /locus_tag="PKV23_05700"
     CDS             2460..>2804
                     /locus_tag="PKV23_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015315257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX84963.1"
                     /translation="MQGVARNFFILGIVMAIAGMMLGLSMAMSHNHVQMPVHAHIMVA
                     GWLMSAVFGFFYHLFPAARQNKLATVHFWIHAVSIVVLVISLYFVLAGNPGVEPVTAI
                     SSIVFFLGMLLFA"
BASE COUNT          582 a          792 c          855 g          575 t
ORIGIN      
        1 cggagcgaat agcgcgcggc gtcggggccg tttcgccccg ggttaacgtg aatacgacga
       61 agttttatct gcccgatgcg attgatgaaa acggactgct caaggccgag atcacggctg
      121 aggccgtcgg ccggccttcc ccggcctaac gcacctgccg ggaattaaca gttcatttac
      181 catagttgca ctgtgggcgg gcggtttccg cccggccagg aaatgaaagt ttttctgaaa
      241 ggccctgtta acctcaaatt tgataggatt ggaaaggttt ccggagagaa ttttccaaat
      301 gaacgtcgct gtcaaatctg ccgctgaggt gcctgcattt tcgggttggg atgagctgga
      361 ccgcaaggcg ccaagctgga gaattgagcg ggaagaggtt cagggcgatg ccattgcacc
      421 gttttttccc gaccagaacg gcggggtata cgagcagctc ctgttcaact ttcctttcat
      481 gatgcatgcc atggatggct cgggccggct gaactcggtc aaccggcaat ggtgcaagac
      541 cctgggctac atgccttccg acgtcatcgg aaaatcattc aacgagctgc tgacgccgca
      601 atcgcgttcg cacctgatca ccacaatcta tcccaagtac ctgcagaccg gcctgtgccg
      661 cggcgaggaa atgttcgtcc accgcaagga cgggacgctg gcaaccgtca gcctgtcgat
      721 gaatgcctac cggggcgaca agggccgcat cgaacggtcg atctgtctga tgcatgatgt
      781 caccgaccag aagattgccg aaatcgcgtc ggttcgcagc gaccagaggt tccgcggggc
      841 atttgccgcc gcggcccatg gcatggcgct ggtttcgccg accgggcaga tcgagctatc
      901 caacgcggcc tttggcgcgt tcctcggccg ggaagatatc gacagatcga ctctgacgtt
      961 cgatgagacc ctgcaccgcg atgaccgggg gcaattcctc aacggcatgc gccagctcct
     1021 gtcgggcgaa gtcccgtcat tgaagcagga actgcgctac acgacgggga cgggcggggt
     1081 tgtacatggt tcaaccagcg tttccatggt caggagcgag aaggcggaaa tcgagcagct
     1141 tgtggtgcag attgtcgatg tctcggaacg caagctgacc acccagcgcc tgcaccaggc
     1201 gcagaagatg gaagccatcg ggcagctgac cggcggtttg gctcacgact tcaacaacct
     1261 gctcaccatc atcatgggca atctgcagtt gcttgagggc aagctggcgg gcgacgagaa
     1321 ggcagtcaag cgtgccagcg aagcgcttga cgcggcacgc aagggctcag acctgacgcg
     1381 ccagctgctg gccttcgcgc gcaagcagga ccttgagccg cgcgaagtga agatcaacga
     1441 cctggtcacg ggcatggagg ccctgatttc acgcacgctt ggcgagaatg tcgaactcaa
     1501 ggtcgatgtg gccgccggtt caccccaggt cctgattgac ccctcgcagt ttgaatcttc
     1561 aatcctgaac ctggcgatca atgccaggga cgccatgccc aatggcggcc tgctgaccat
     1621 tgaaacccaa ctggtccatc tcgaccgcga atatgcggac cggcatccgg aagtcgttcc
     1681 cggcgatcat gtcctggtgg cgatttcgga tacgggcatc ggcatgtcgc cggaactgct
     1741 ggaaaaggtc ttccacccgt tcttcaccac caaggaaaat ggcaagggca ccgggcttgg
     1801 cctttccatg gtctatggct tcatcaagca gtcgggcgga cacatcagca tttacagcga
     1861 agtcgggcac ggcacctcgg tcaaaatgta tctgccgcgg aaaatgctgg ccggtgaaac
     1921 cgccgatcct ccggctgcgg agaattattc tgccgacacg gcgccggcca ttgcagcgcc
     1981 agcggaaact gaagttccca aaaaggcgcc gcggccgcag aagatcctcg tggtggaaga
     2041 ccaggaagcg gtgcgcgccg tggcttgcgg cttcctcatg gatttcggct atgacgtggt
     2101 ggaagcagag gacggcttcc aggcgctttc ccgccttcag gaagatcccg agatcgacct
     2161 catgttctcg gatgtcgtga tgcccggcgg aatgaacggt ttcgacctcg cccaggcagc
     2221 cagcggcatg cggcccgaac tcaagattgt ccatacctcg ggctatccca agggcgcgat
     2281 ggtccaccag gacgagccgc gcttccgcca gggcttcatc atcatgaagc cgtaccgccg
     2341 cgaggagctg cagaaaatca tccgcgacgc attcgaaaag cagggctgag gctttttaga
     2401 atggttcgaa tctgctagga attgcgccgc aggaagcgat tcttagcagg ggagcgaaca
     2461 tgcagggtgt tgcaaggaat ttctttatac tcggaattgt catggcgatt gccgggatga
     2521 tgctcggtct tagtatggcg atgagccaca atcatgttca aatgccggtc catgcccata
     2581 tcatggtggc gggctggctg atgtcggcgg tgttcgggtt cttctatcac ctgtttccgg
     2641 cggcccggca aaacaagctg gccacggttc atttctggat ccatgccgtc agtatcgtcg
     2701 tgctggtcat ttcgctttat tttgtcctgg cgggcaatcc gggggttgag ccggtgacgg
     2761 caatttcatc cattgtgttt ttcctgggca tgctgctgtt tgcg
//
LOCUS       DAORVN010000238         2802 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_33203_length_2802_cov_3.068802, whole genome shotgun sequence.
ACCESSION   DAORVN010000238 DAORVN010000000
VERSION     DAORVN010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2802
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33203_length_2802_cov_3.068802"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="PKV23_05705"
     CDS             <1..219
                     /locus_tag="PKV23_05705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX84964.1"
                     /translation="VQVGHVSFPVCRVQPEQFLGNHQRKDPVTEEFQALVVVTAVIGA
                     AVGQPVAQENLVLEFIAKALRDFVMQGL"
     gene            complement(243..1043)
                     /gene="lepB"
                     /locus_tag="PKV23_05710"
     CDS             complement(243..1043)
                     /gene="lepB"
                     /locus_tag="PKV23_05710"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018697681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HQX84965.1"
                     /translation="MAPAQDMRQKSEEGVWETVKVIIQALLIAFFVRTFFYQPFNIPS
                     ESMYPTLKVGDYLFVSKLSYGYSKYSFNFSFGGFGTTWITCCPIDFPGRVVLADTPKR
                     GDVAVFKLPTDTNIDYIKRVIGLPGDRIQMRDGVLFINGEMVKKERIEDYVDGEVMGE
                     GGGHPIQQYEETLPNGVKYRVLDQVPNGSADNTIEYVVPPAHYFMMGDNRDNSEDSRF
                     LDVVGYVPIENYVGRADIIFFSISRGTTLWEVWKWPFEIRWGRFLNLL"
     gene            complement(1175..1576)
                     /gene="acpS"
                     /locus_tag="PKV23_05715"
     CDS             complement(1175..1576)
                     /gene="acpS"
                     /locus_tag="PKV23_05715"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018687442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HQX84966.1"
                     /translation="MIIGLGNDMVDIRRIEQTLERYGTRFVARIFTDIEQKKSDRRAQ
                     RAASYAKRFAAKEACSKALGTGFRRGVFWKDMGVVNEPSGRPTLQLTGGAKAQLERIT
                     PAGHSARIQLTITDDYPYAQAVVIIEAVAAS"
     gene            complement(1573..2163)
                     /gene="pyrE"
                     /locus_tag="PKV23_05720"
     CDS             complement(1573..2163)
                     /gene="pyrE"
                     /locus_tag="PKV23_05720"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017486439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HQX84967.1"
                     /translation="MLSHDEVLGEFRAAGALLEGHFILSSGLHSSRYLQCARVLMDPR
                     RSERLCQALATRIGEKIRSQIDLVASPAMGGVVVGYEMGRQLGVPAIFFERVEGKLVL
                     RRGFAIAKGARVLMVEDIVTTGLSSRECIAGIAEEGGVTLAAACLIDRSGGKADLGVP
                     LFALTSLEIPAYPADALPPELAAIPAIKPGSRGLAK"
     gene            complement(2167..>2802)
                     /locus_tag="PKV23_05725"
     CDS             complement(2167..>2802)
                     /locus_tag="PKV23_05725"
                     /inference="COORDINATES: protein motif:HMM:NF040246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5913 domain-containing protein"
                     /protein_id="HQX84968.1"
                     /translation="AAAVPRLGHKNVDDTMAAIGRGDLSGYDMLKAMGLTVEASQMRE
                     TRRKGTAAKKLDPAHAASVPVRGLSGNMAFTISKETGAVPGERIVGITMPGEGVTIYP
                     IFARALEQFDSQPERWIDLAWDSAASDQKFPARINIVLLNEIGALAQVTQVIGDQGGN
                     IDELQMMARQGVRDFFDLDILLEVHDARHLNDIIAGLRTKNAVSAVTRATG"
BASE COUNT          583 a          885 c          770 g          564 t
ORIGIN      
        1 gtgcaggttg gccatgtgtc cttcccggtg tgccgggttc agccggaaca gttcctcggc
       61 aatcaccagc gcaaggaccc ggtcaccgag gaattccagg cgctggtagt cgtcacggct
      121 gtgattggtg ctgctgtggg tcagcccgtg gcgcaggaga accttgtcct tgaattcata
      181 gccaaggcgc tgcgtgattt cgtcatgcag ggcttgtgag aaccggacca acccccgcgc
      241 gcctagagaa gattcaggaa acggccccag cgaatttcga aaggccactt ccaaacttcc
      301 caaagggttg tgccgcgcga aatcgaaaag aagatgatgt ccgcccgtcc cacatagttt
      361 tcaatgggca catagcccac cacatcgagg aaccgcgaat cctccgagtt gtcccggttg
      421 tcgcccatca tgaaataatg cgccggcggc accacatatt cgatggtgtt gtcagccgag
      481 ccgttgggta cctggtccag cacccggtat ttcacgccat tgggcaaagt ctcttcatac
      541 tgctggatgg gatgcccgcc gccctcgccc atcacctcac cgtccacgta atcctcaatg
      601 cgctccttct tcaccatttc cccgttgatg aagagaaccc cgtcgcgcat ctggatgcgg
      661 tcgcctggta ggccaattac ccgcttgatg taatcaatgt tcgtgtcggt cggcagcttg
      721 aacaccgcca cgtcgccgcg cttgggcgta tccgccaaaa ccacccgccc cggaaaatca
      781 atggggcagc aagtgatcca cgtggtgcca aagccgccga acgagaaatt gaaggaatat
      841 ttgctgtagc cataggaaag cttggagacg aaaagatagt cgccgacctt cagggtggga
      901 tacatggact cggaaggaat gttgaacggc tggtagaaga aggtccgcac gaaaaaggca
      961 atcagcagtg cctggatgat gaccttgacg gtctcccata ccccctcttc ggatttctgg
     1021 cgcatatcct gggccggtgc catgtgatag tctttcttgt gcatcgaaat cgagctttcc
     1081 gcgctctata tatactttgg gctatagcgc ctccaccata tgaggtgcaa tgtagcacca
     1141 tgcaagctcc ggcactgtga cgaattaatc acagtcagga agccgccacc gcctcgatga
     1201 taacaacggc ctgggcatag gggtaatcat cggtgatcgt gagctgaatg cgtgctgaat
     1261 gtccggctgg agtgattcgc tccaattgcg ccttggcccc gcccgtaagc tgcaaggtgg
     1321 gacgtcctga aggctcgttc acaaccccca tgtccttcca gaaaaccccc cggcgaaaac
     1381 ctgtgccaag ggccttggaa caggcctcct tggcggcaaa tcgcttggca taagaagccg
     1441 cccgctgcgc ccgccggtcg gatttcttct gctcgatatc ggtgaaaatg cgcgccacga
     1501 aacgcgtccc gtagcgctcc aaagtctgtt caatgcgcct gatatccacc atgtcattgc
     1561 caagtccgat gatcatttgg caaggccccg gctgcccggt ttgattgccg gaatggctgc
     1621 cagttccggc ggcagtgcat cggccggata ggccggaatt tcaaggctgg tcagcgcaaa
     1681 cagcggcacg cccaaatccg ccttgccgcc ggagcggtcg atgaggcagg ccgcggccag
     1741 cgtaaccccc ccctcctcgg cgatgccagc aatgcactcc cgcgatgaaa gccctgtagt
     1801 aacaatgtct tccaccatca gaacccgggc gcccttggca atggcgaagc cccgtcgcag
     1861 taccagtttt ccctccaccc gttcgaagaa aatggcagga accccaagct gccgccccat
     1921 ttcatagccc accacgacgc cacccatggc cggcgacgcc acgaggtcaa tctgcgaccg
     1981 gattttctca ccgattcgcg ttgctagggc ctggcaaagg cgctccgacc gccgaggatc
     2041 catcaaaacc cgggcgcatt gcaggtagcg cgaactgtgc aggccggagg acagaatgaa
     2101 atgcccttcg agaagagcgc ctgccgcgcg gaattcgccc aatacctcgt cgtggcttaa
     2161 catctgctac cccgttgccc gtgtgaccgc gctcaccgca ttcttggttc tgagcccagc
     2221 gatgatatcg ttgagatgcc gtgcatcatg cacttccagc agaatgtcca gatcaaaaaa
     2281 gtcgcgaact ccctgccgtg ccatcatctg caattcatcg atgttgccgc cctggtcgcc
     2341 gatcacctgg gtgacttgcg ccagggcgcc gatctcgttc agaagcacga tattgatccg
     2401 cgccggaaac ttctggtccg aggcggcgct gtcccaggca agatcgatcc agcgttccgg
     2461 ctggctatcg aactgctcca gtgcccgggc gaaaatcgga tagatcgtga caccctcgcc
     2521 gggcatggta atgcccacaa tgcgctcgcc cggaaccgcg ccggtttcct tggaaatcgt
     2581 gaaggccatg ttgccgctca ggcctcgcac cggaaccgac gccgcatggg ccggatccaa
     2641 tttcttggct gccgtcccct tgcgccgcgt ttcccgcatt tggcttgctt ccaccgtgag
     2701 ccccatggct ttcagcatgt cgtaacccga aaggtcgcca cgcccgatcg ccgccatcgt
     2761 gtcgtccaca ttcttgtggc caaggcgcgg caccgcggcg gc
//
LOCUS       DAORVN010000239         2798 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_33308_length_2798_cov_2.488881, whole genome shotgun sequence.
ACCESSION   DAORVN010000239 DAORVN010000000
VERSION     DAORVN010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2798
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33308_length_2798_cov_2.488881"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1068)
                     /locus_tag="PKV23_05730"
     CDS             complement(<1..1068)
                     /locus_tag="PKV23_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018642547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQX84969.1"
                     /translation="MSDLQPKDHPLALTPVVRLDTVGLSYGKTRALGSITLNMPAGCM
                     VGLIGPDGVGKSSLLSLIAGARAIQQGRITVLGGDIADASHRAMACPRIAYMPQGLGK
                     NLYPTLSVFENVDFFGRLFGQDRQERERRIGELLQSTGLAPFADRPAGKLSGGMKQKL
                     GLCCALIHDPDLLILDEPTTGVDPLSRRQFWELIDRIRYDRLGMSVIVATAYMEEAAR
                     FDWLVAMDSGKVLATGTPTDLLEQTGALNLDAAFIGLLPEKERADYREVVIPPRSFEH
                     DEEIAIEAEHLTVRFGDFTAVDDVSFRISRGEIFGFLGSNGCGKSTTMKALTGLLSTS
                     QGNARLFGHQVDPNDMTVRRRV"
     gene            complement(1065..2141)
                     /locus_tag="PKV23_05735"
     CDS             complement(1065..2141)
                     /locus_tag="PKV23_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011159623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HQX84970.1"
                     /translation="MTITKSTKPWLIAALVAILAGGGYYAWQKFLRSDPLEGIARGNG
                     RIEAIEIDVSTKAPGRVKEILVNEGDFVTAGQVLARMDTEQLEAQRRQAEAQLHRAKI
                     SIDTAKSLVIQREAERTAAASVVAQREAQLDSIQRKLARSEQLITTNAVSQQVLDDDR
                     ANTQGAKAAVATAKAQFAASEAAISAANAQVVDAGAAVEAAKASLESIGADINDSILK
                     SPRDGRVQYRVAQPGEVLSAGGRVLNLVDLGDVYMTFFLPTAQAGRVAIGADVRLILD
                     AAPQYVIPAKVTFVADVAQFTPKTVETEEERLKLMFRVKAQIAPELLRKYIQQVKTGL
                     PGMAYVRLDQKVEWPASLQRTLAQ"
     gene            complement(2259..2588)
                     /locus_tag="PKV23_05740"
     CDS             complement(2259..2588)
                     /locus_tag="PKV23_05740"
                     /inference="COORDINATES: protein motif:HMM:NF020918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HQX84971.1"
                     /translation="MAIENDSPEAQAPVADLKYLFFSSTEWLKSFSENPRRLYGVWNQ
                     GLSFTASRLQEQADYAKALSECTDVAEALKYSAEFMQNAWKKSYSDGSKFFEAWRENL
                     PSAAQGK"
BASE COUNT          596 a          813 c          770 g          619 t
ORIGIN      
        1 gacacggcgc cggacagtca tatcgttcgg atccacttgg tgtccgaaca gcctcgcgtt
       61 gccctggctg gtcgacagca gtccagtcaa cgccttcatt gtcgttgatt tgccgcagcc
      121 gttcgagccg aggaagccga agatttcgcc tcggctgatg cgaaagctga cgtcatcaac
      181 cgccgtgaag tctccaaagc ggacggtaag atgctcggcc tcgatggcaa tctcttcatc
      241 gtgctcgaag gagcgaggtg gaatcaccac ttccctgtaa tccgcacgct ccttttccgg
      301 gagcaggccg atgaatgcgg catcgagatt aagagcgccg gtctgctcca gcaggtctgt
      361 gggcgtaccg gttgcgagca ccttgcccga atccatcgcc accagccaat caaagcgcgc
      421 agcctcttcc atataggcgg tcgcgacaat gacgctcata cccaagcggt cgtaccgaat
      481 ccggtcgatc aactcccaga attgccgccg tgacaaagga tcgacgccgg tcgttggttc
      541 atcgaggatc aggagatcag gatcatggat cagggcgcag caaagaccaa gcttctgctt
      601 cataccgccc gaaagtttac ccgctggtcg atccgcaaaa ggagccaggc cggtactttg
      661 caacagttcg ccgatgcgac gctcgcgctc ttgcctgtct tgaccgaaca aacggccaaa
      721 aaagtcaacg ttctcgaata ccgaaagtgt cggatagagg ttctttccga gtccctgcgg
      781 catatacgca atgcgcggac aagccatcgc ccggtgagat gcgtcggcaa tgtctccacc
      841 cagcacggta atacggcctt gctgaatggc gcgagcgccc gcaatcagcg aaaggagact
      901 ggacttgccg acaccgtcag gtccgatcag cccgaccatg cagcccgccg gcatattgag
      961 cgtaatggag ccgagtgccc gggtctttcc gtatgacagc ccgacagtgt ccaggcggac
     1021 gaccggcgtc aaagccaaag ggtggtcttt gggctgcagg tcgctcattg tgccagcgtt
     1081 ctctgcagac tggctggcca ttccactttc tggtcgagcc ggacataggc catgcccgga
     1141 aggccggttt tgacctgctg gatatatttt cggagcaatt ccggagcaat ttgtgccttg
     1201 actcggaaca taagctttag ccgctcttct tcagtctcga ctgtcttggg cgtgaactgg
     1261 gcgacatcgg cgacgaaagt gactttagcc ggaatgacat actgaggagc ggcatcaagt
     1321 atgagacgca catcggcgcc aattgcgacg cgccccgcct gcgctgtcgg caagaaaaaa
     1381 gtcatgtaga cgtcgccgag atcaacgaga ttgagaaccc gcccacccgc ggaaagaact
     1441 tcgccgggct gcgccacacg atattgaacg cgcccgtcgc gaggtgattt taagatacta
     1501 tcgttgatat cggcgccgat actctcgagg gaggctttgg ctgcttcgac cgcggcaccg
     1561 gcatcaacca cctgtgcgtt tgctgcactg atcgcggcct ccgacgcggc aaattgtgct
     1621 tttgccgttg ctactgcagc ctttgcccct tgggtattcg cccggtcgtc gtccagcacc
     1681 tgctgagaaa ctgcgttggt ggtgattaac tgctcggatc gcgcaagctt tctctgaatt
     1741 gagtcaagct gcgcctcgcg ctgggcgaca accgaagccg ccgccgttcg ttcggcctcc
     1801 cgctgaatga caaggctctt ggcggtatca atgctgattt ttgcccgatg cagttgcgcc
     1861 tcggcctggc gacgctgcgc ttcgagctgc tccgtatcca tacgggcaag cacctgcccg
     1921 gcggtaacga aatccccctc attgaccaat atttctttga cgcgtcccgg cgctttagtc
     1981 gacacgtcaa tctcgatcgc ctcgatccgg ccgttgccac gcgctatccc ttcaagagga
     2041 tcgcttctga ggaatttctg ccaagcataa tagccgcccc cggcgaggat cgcgacgaga
     2101 gcggcaataa gccaaggctt tgtggatttc gtaatggtca tcaattgccc ttcttgtgaa
     2161 tctgctcagc agcgcatcgt tcgcgaaatg ctagactgcc tgttcggata acgtctagca
     2221 gcacgaaatg tcatcccgcg tacgggcaag gataaaggtt acttgccctg cgccgcggaa
     2281 ggcagattct cgcgccaagc ctcaaagaat ttcgatccgt cactgtagga tttcttccat
     2341 gcattttgca tgaactcagc ggaatatttt agcgcttcgg cgacatccgt gcattcggat
     2401 agcgcctttg cataatcggc ctgttcctgg aggcgagatg cggtgaagct aagaccttgg
     2461 ttccacacgc catataatct tctaggattt tcagaaaaag acttcagcca ctctgtactg
     2521 ctgaagaaaa ggtatttcag atcggccaca ggtgcttgcg cttcagggga gtcgttctcg
     2581 atcgccattg ccgtctccat gctctgccta attttctaat cgcgtaactt tagctaaggc
     2641 gacagagcgc gatgttgacc cacatcaacc actgttgacc agatttgact gtacgatagt
     2701 agtgcgatat ggcaactggt ttttgataac gtcgcggagg ccgatctggc cgatggcgcg
     2761 cgcgcactcg acgaagagaa cggcgccaga attttagc
//
LOCUS       DAORVN010000240         2796 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_33338_length_2796_cov_3.856257, whole genome shotgun sequence.
ACCESSION   DAORVN010000240 DAORVN010000000
VERSION     DAORVN010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2796
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33338_length_2796_cov_3.856257"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..845
                     /locus_tag="PKV23_05745"
     CDS             <1..845
                     /locus_tag="PKV23_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018011287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HQX84972.1"
                     /translation="AVGEALFMASQARLRTAEILPIKGSLFMTREEWRRSWKPWLRGA
                     GIGFPFGALPAGGTEIPTFLSYFLEKKLTKHPEEFGRGAIEGVAGPEAANNAAVAGVL
                     VPLLALGLPTSATAAILLAAFEQYNLQPGPLLFAGNSTLVWGLIASLYIGNVMLLVLN
                     LPLVGLWVRLLTIPQPLLYGGILIFSTLGVYSLGNSTFDLVLLFIVGFIGFLMRRHEF
                     PVAPCIIGLILGPFAEAHFRRALSISQGDPSVFVTHPLSAVLLATAAAVIIVPIILRR
                     IKYA"
     gene            972..2522
                     /locus_tag="PKV23_05750"
     CDS             972..2522
                     /locus_tag="PKV23_05750"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HQX84973.1"
                     /translation="MAPPLRPSLFRSETLATALILAFLVFLMGGTAFVLRNAYQSAAA
                     IARIRAISSAEVVAANFEWVGETSQQLLQRVDDFVGPSLDALPANALGFLKESVSTLP
                     GSPRIYLVGADGNTRLTTDPDFKPIDIRDRDYFQAVADGAARYISPMLVSRLNGEQIF
                     VISKRVERNGVFVGAAIVSFNSQLIEKVWSSLRFDPTSTISAVREDGKIVARYPRLES
                     RTDISDSVLFTTFLPQAPNGTYEAVSVADGVKRIVAYRRVGGSNLVAIASIGHDVAFA
                     AFYNFAILSLAVAVPLALFLAALAWITFQWLSRDARQREQLAAALESNQMLFREIHHR
                     VKNNLQAVSSLVNLQKIDPHAKQEMTQRIQAMVAVHEQIYRNDQFGLVDASAYIPAII
                     GKLVESYGREVKVAYDLAPMEVDREHALPLGLVANEVVSNAMKYAFQDGRDGALTVTL
                     KKSEDGRMGILTIHDNGAGFDPESISKGTGSRLVSGLAAQIQGETGYTFDSGTKFEMR
                     FPLPNASA"
     gene            complement(2514..>2796)
                     /locus_tag="PKV23_05755"
     CDS             complement(2514..>2796)
                     /locus_tag="PKV23_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007689174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQX84974.1"
                     /translation="FEAPDADRAIKILEANSAIRVLFTDIDMPGSMDGLKLAAAVRDR
                     WPPIRIVVASGLRKINVDSLPADSRFFTKPYSVIEIAATMRSMVQAPLG"
BASE COUNT          523 a          823 c          830 g          620 t
ORIGIN      
        1 tcgccgtagg cgaggctctc ttcatggcct cgcaggcgcg cctgcgcacg gcggagatcc
       61 tgcccatcaa gggttcgctc ttcatgacgc gggaggaatg gcgccggtcg tggaagccct
      121 ggctgcgcgg cgccggcatc ggctttccat tcggcgcgct gccggccggc ggcacggaga
      181 ttcccacctt cctgtcgtac ttcctcgaaa agaaactcac caagcatccg gaagaatttg
      241 gccgcggcgc catcgaaggt gtggctggcc cggaagcggc caacaacgcg gcggtggcgg
      301 gggttctcgt tccgctgctg gcccttgggc ttcccacttc ggcaacggcc gcaatcctgc
      361 tggcggcttt cgagcagtac aacctccagc cgggaccatt gctgttcgcc ggcaattcaa
      421 ccctggtctg gggcctgatc gccagcctct acatcggcaa cgtcatgctg ctggtgctca
      481 acctgccgct ggttggcctg tgggtacgcc tgctgaccat tccccagccc ctgctctatg
      541 gcggcatcct gatcttctcg actctcgggg tttattccct gggcaattcg acttttgatc
      601 tggttctgct gttcattgtg ggcttcatcg gcttcctgat gcggcgccac gagtttccgg
      661 ttgccccctg catcatcggg ctgatcctcg gcccgtttgc cgaggcgcac ttccgccgcg
      721 ccctgtcgat cagccagggc gatccttcgg tttttgtgac ccatccgctt tccgccgttc
      781 tgctggctac cgccgcggct gtcatcatcg tgccgatcat cctgcgacgg attaaatatg
      841 catagttccc cgcacttcgg tgagcttgcg taaggccccg aacgctgtgt aaatcactgc
      901 atcgatgcac tgcttctttt tacgataccg gaatgccccg gcactcaaaa ttctggaaat
      961 aatgtgacct gatggcaccc cccttgcgcc cttcgctgtt tcgctccgaa accctggcga
     1021 cggcactcat tcttgcattt ctggttttcc tcatgggcgg cacggccttt gttctgcgga
     1081 acgcctatca atcggccgct gcaatcgcgc gcataagggc catttcttcg gccgaagtgg
     1141 tggcggccaa ttttgaatgg gtgggcgaga cttcgcagca gcttttgcaa agggtcgacg
     1201 attttgtcgg ccctagcctt gacgcgcttc cggcaaatgc cctgggattc ctcaaggagt
     1261 ccgtttcaac gcttcccggt tcgccgcgca tctatctggt aggcgctgac ggaaacacaa
     1321 ggcttacgac cgacccggat ttcaagccca tcgacatccg ggaccgggat tatttccagg
     1381 ccgtcgcgga tggggcggcg cgctacattt cgcccatgct ggtgagccgg ctcaatggcg
     1441 agcagatctt cgtaatcagc aagagggtcg aacggaacgg cgttttcgtg ggcgcggcta
     1501 tcgtgtcgtt caactcgcaa ctcatcgaga aggtgtggag ttcgcttcgt tttgacccaa
     1561 cttcgacgat ttcggcggtt cgcgaggatg gcaagattgt tgcccgctat ccaaggttgg
     1621 aaagccgcac cgatatcagc gattccgtcc tgttcacgac cttcctgccg caggcgccaa
     1681 acggaaccta tgaagccgtt tcagttgcag atggggtgaa acgcatcgtt gcctaccgca
     1741 gggtcggagg atcaaatctt gtcgcaatcg cctcgatagg gcatgacgtg gcctttgccg
     1801 ctttctacaa ttttgcaatt ttgagcctcg ccgtcgcggt gccgcttgct ctgtttctcg
     1861 cggcgcttgc ctggatcaca ttccagtggc tgtcgaggga cgcgcggcaa agagaacaac
     1921 ttgccgcggc gcttgaatcc aaccagatgc ttttccggga aattcatcac cgggtcaaaa
     1981 acaacctgca ggcagtgagt tcgctggtca acctgcagaa gatcgatccc catgcaaagc
     2041 aggaaatgac ccagcgcatc caggcgatgg ttgcggtgca tgagcagatc taccgcaatg
     2101 accagttcgg gctggtggat gcatcggcat atattccagc catcatcggg aagctggttg
     2161 aatcctacgg gcgcgaggtg aaggtggcct atgatctggc gcccatggaa gttgaccgcg
     2221 aacatgccct gccgcttggg ctggttgcaa atgaagtggt gtccaatgcg atgaagtatg
     2281 cgtttcagga tggcagggac ggcgcactga cagttaccct gaagaaatcg gaagacgggc
     2341 gcatgggcat tttgacgatc catgacaacg gtgccgggtt tgatccggag tcaatctcca
     2401 aaggcacggg cagcaggctg gtcagcgggc tggcggcgca aatccagggc gagaccggat
     2461 acaccttcga cagcggcaca aaattcgaga tgcgctttcc cttgcctaac gcctcagcct
     2521 agcggggcct gcaccatgga gcgcatggtg gcggcaattt caatcacact atagggcttg
     2581 gtgaagaagc gggaatctgc aggcaaggaa tccacgttga ttttccggag gccggaggca
     2641 acgacaatcc ggatcggcgg ccagcggtca cgcacggcgg cggcgagctt aaggccatcc
     2701 atgctgcccg gcatatcgat atcggtgaag aggactcgta tggcggaatt ggcttcgaga
     2761 atcttgatgg cacggtcggc atcaggcgcc tcgaaa
//
LOCUS       DAORVN010000241         2792 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_33433_length_2792_cov_3.150530, whole genome shotgun sequence.
ACCESSION   DAORVN010000241 DAORVN010000000
VERSION     DAORVN010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2792
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33433_length_2792_cov_3.150530"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..747
                     /locus_tag="PKV23_05760"
     CDS             <1..747
                     /locus_tag="PKV23_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009211309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HQX84975.1"
                     /translation="EKADWVLLTLNKHGVDTSAMQRLKGVPTSATILNVRPNGDRPAL
                     HVRGASDHFDVAPGMYGQVFDAPIIHLGGTGLLKKLDGPASVTLLKEAKKRGRTVTFD
                     LIAASAETIGIVGPLLPYIDYFMPSIEEAKDMSGQSTAEDCAKFFLDRGAACCVFTLG
                     GDGAYYAHKDGTRLTSPAYDIRIVDTTGCGDAFDAGFITSLHHKMDTDTALRFAQASA
                     GLVATGLGSDAGIVSFEDTLQKMRNWKIKS"
     gene            744..1436
                     /locus_tag="PKV23_05765"
     CDS             744..1436
                     /locus_tag="PKV23_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008127668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HQX84976.1"
                     /translation="MRFSGRRAVVTGGASGIGEAVAKRLAAEGGNVAVWDLNGGIKVD
                     ISDFASVEKAVKETVKQIGGIDILVNSAGITGPTVPLMEFPIDGWKQVIDINLNGTFY
                     CNRAVAPLMVAQNYGRIVNIASIAGKEGNPNASGYSASKAGVIGLTKSLGKELAKYNI
                     TVNAVTPAAVRTPIFDQMPQTHIDFMLSKIPKGRFGTVDEMASLICWLASEESSFSTG
                     AVFDLSGGRATY"
     gene            complement(1433..2203)
                     /locus_tag="PKV23_05770"
     CDS             complement(1433..2203)
                     /locus_tag="PKV23_05770"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007199240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HQX84977.1"
                     /translation="MAEWIKRFSLAGKTAVVTGATKGIGFETCKVLADAGADIAAVGR
                     DRAGLAEVAAAVKSLGRRCFAIEADLASADEPVTAARAALKEFGSIDILVNNAAVALI
                     APLLRLSVEDWDTTMAVNLRAPFLIARELAPQMIARKAGKIINVSSQAGIIAVDDHGA
                     YSSSKGGLNMLTKAMTVEWAKHNIQINSVCPTVILTPMGEKNWGSPEKSGPMLAKIPA
                     GRFGKPVEVADMILYLASSASDLVCGQDILIDGGYTAL"
     gene            2271..2600
                     /locus_tag="PKV23_05775"
     CDS             2271..2600
                     /locus_tag="PKV23_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003505301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HQX84978.1"
                     /translation="MERMAQIIKVKPEVIPEYKRIHAAVWPEILKAISDSNIRNYTIF
                     LKEPENILFAYWEYHGSDFKADMEKIKKAPRMREWWDITDPMQIPYETRKPGDWWAVM
                     ENVFHTD"
     gene            complement(2605..>2792)
                     /locus_tag="PKV23_05780"
     CDS             complement(2605..>2792)
                     /locus_tag="PKV23_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018391582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HQX84979.1"
                     /translation="WMNYVSGPRLWPVTADNTTFGVWTGDWVASPQDDLTLIPRTEEN
                     VYQRAFAELEKNFFGKK"
BASE COUNT          584 a          905 c          762 g          541 t
ORIGIN      
        1 gaaaaggccg attgggttct gctcaccctc aacaagcacg gcgtcgacac gtcagccatg
       61 cagcgcctca aaggcgttcc cacttctgcg acaatcttga atgtgcgccc caatggcgac
      121 cggcccgccc ttcatgtgcg cggcgcttct gatcatttcg atgtggcacc gggcatgtat
      181 ggccaggtct tcgacgcccc tatcatccat ctcggcggaa ccgggctgct gaaaaaactc
      241 gatggtcccg ccagcgtcac tctcctcaag gaagccaaga aacgcggacg caccgtgacc
      301 ttcgatctca tcgccgccag cgccgaaacc atcggcatcg tcggcccgct cctgccctat
      361 atcgactatt tcatgccctc gattgaggaa gcaaaggaca tgtccggcca gtccaccgcc
      421 gaggactgcg ccaaattctt cctcgacagg ggggctgcct gctgcgtctt caccctcggc
      481 ggtgatggcg cctactatgc ccacaaggac ggcacgcggc tcacttcgcc cgcctatgac
      541 atcaggatcg ttgacaccac cggctgcggc gatgcctttg atgcgggctt cataacgagc
      601 ttgcaccaca agatggacac cgacaccgcg ctccgttttg cgcaagcctc ggccgggctc
      661 gttgccaccg ggctggggtc cgacgccgga atcgtttcct tcgaagacac ccttcaaaaa
      721 atgagaaact ggaagataaa gtcatgagat tttcaggcag gcgggcggtg gtaacgggcg
      781 gcgcctcggg cattggcgag gccgtggcca agcgccttgc ggcggaaggc ggcaacgtcg
      841 ccgtatggga cctcaacggc ggcatcaagg tggatatttc cgatttcgcc tcagtcgaga
      901 aggccgtgaa ggaaaccgtc aagcagatcg gcggcatcga cattctggtg aacagcgccg
      961 gcatcaccgg acccaccgtt cccctcatgg aatttcccat cgatggctgg aaacaggtga
     1021 tcgacatcaa cctgaatggc actttctatt gcaaccgcgc cgtggccccg ctcatggtgg
     1081 cgcagaatta cggccgcatc gtcaacatcg cctccatcgc cggcaaggag ggaaacccca
     1141 atgcctccgg ctattccgcc tcaaaggccg gcgtcatcgg cctgaccaaa tcgctaggca
     1201 aggaactggc caaatacaat atcaccgtga acgccgtcac gcccgccgcc gtgcgaaccc
     1261 cgatcttcga ccagatgccg caaacccata ttgacttcat gttgtcgaaa attcccaagg
     1321 gccgcttcgg caccgtcgat gaaatggcaa gcctcatctg ctggctggcc tcagaggaaa
     1381 gctccttctc gaccggcgcg gtcttcgatc tctccggtgg ccgcgccacc tactagagcg
     1441 ccgtatagcc gccgtcgatg aggatgtcct ggccgcagac aaggtccgac gccgaagacg
     1501 ccaggtagag aatcatgtca gccacctcaa ccggcttgcc gaagcgcccg gccggaatct
     1561 tggccagcat ggggccggac ttctccgggc tgccccagtt cttttcgccc atcggcgtga
     1621 ggattaccgt cgggcagacc gaattgatct ggatgttgtg cttggcccat tcaaccgtca
     1681 tggccttggt cagcatgttg aggccaccct tcgaagagct gtaggctccg tgatcgtcaa
     1741 ccgcgatgat gcccgcctgc gacgacacat tgatgatctt gccggctttc ctggcaatca
     1801 tctggggcgc cagttcccgc gctatcagga acggcgcccg caggttgacc gccatcgtcg
     1861 tatcccagtc ctcgacagaa agcctgagca gcggcgcgat caacgcaact gccgcattgt
     1921 tgaccaggat gtcgatggat ccgaattcct tcagcgctgc ccgcgccgcg gtcacgggct
     1981 cgtcggcact tgcaagatcg gcttcaatgg cgaagcatct gcggcctagg cttttgaccg
     2041 ccgcggcaac ctccgcgagc cctgccctgt cacgcccaac cgccgcgatg tcggcgccgg
     2101 cgtccgccag caccttgcag gtttcaaagc cgatgccctt cgtggcgccc gtgaccacag
     2161 cggttttccc cgcgaggctg aatcttttta tccactcggc cattgtttcc ctcccgctgt
     2221 ccgctaatct ttccatgcaa tatcaattgc cgcaagcgag gaatgggcac atggaaagaa
     2281 tggcgcagat catcaaggtg aagccggaag tcattcccga atacaagcgc atccacgccg
     2341 ccgtctggcc tgaaattctc aaggccattt cggactcgaa cataaggaac tacacgatct
     2401 tcctgaagga gccggaaaac atcctcttcg cctactggga ataccacggc agcgatttca
     2461 aggccgacat ggagaagatc aagaaggccc cgcgcatgcg ggaatggtgg gatattaccg
     2521 atcccatgca gatcccctac gaaacccgca aacccggcga ctggtgggcc gtgatggaaa
     2581 atgtgttcca caccgattga aacgtcattt cttcccgaaa aaattcttct ccaattcggc
     2641 aaaggcccgc tgatacacat tttcctccgt gcgcggaatg agcgtgaggt catcctgcgg
     2701 gctggctacc cagtctccgg tccacacccc gaatgtcgtg ttgtcggcgg tcaccggcca
     2761 aagccttggc cccgaaacat aattcatcca gg
//
LOCUS       DAORVN010000242         2781 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_33676_length_2781_cov_3.284299, whole genome shotgun sequence.
ACCESSION   DAORVN010000242 DAORVN010000000
VERSION     DAORVN010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2781)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2781)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2781
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33676_length_2781_cov_3.284299"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..815)
                     /locus_tag="PKV23_05785"
     CDS             complement(<1..815)
                     /locus_tag="PKV23_05785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX84980.1"
                     /translation="MPVQQPCAPVWAALALALLLFCFYNLNFRFGDSPDTIPATVLPA
                     SILAEGTFNLDRSRSLLANNDNALDICLKYFNSIQERNGHLVSSYPPGAGIMAVPVYA
                     IAKYSGHLKQWYQYRLSGKIAASLMVALSAVFIFLALSLTIETRAAWMIALFYGLGTS
                     AWSVSSQALWQHGPSALCLAIATYALYRQECRPSNLLAAVAGLFLGMAVFCRMTNFIP
                     AGALTLFMLFHQRKYLPAYLLPLLCMAGIIAYYNLSTFGDLKGGAGVLLASKV"
     gene            complement(888..2678)
                     /locus_tag="PKV23_05790"
     CDS             complement(888..2678)
                     /locus_tag="PKV23_05790"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HQX84981.1"
                     /translation="MNQKMICLIKPPAVECFRLAPATFKISPPLGLAYIAGALQAAGR
                     RVTVIDSVAESPGEMTRYYKGYLIGLPLAEVAARIPADASLVGISVIFTHEWPEIVHL
                     IDLIKQRHPDLTVVLGGEHITAMPEFCLSTSKADILVLGEGEETIIELADALETGRPL
                     DEIDGLAFRDRDHNRIIVNQRRKRMTEVDTLASPAWDLFDLKTYHEQRYVGGIFSASL
                     SVPILATRGCPYQCTYCSAPNMWTPRWDPRDPVKVVDEIEGYIHKYGAGNFPLQDLTA
                     IIKKDWVVTFCQEILRRNLKITWQLPTGTRAEAIDEEVAQLLFKSGMINMAYAPESGS
                     ETTRKLIKKKMHTDRLFESLRAAVSAKLNVVAFLVVGFPHDTREHLMENMPFLDQLAA
                     EGLTDVSVGFYMALPGTELFYSLYDAGKIRINREYFSHILHSAMLWPAQSYSENLSRF
                     AQAAMKMRIFLRFYGARRSSTERAPLLLALLRTLKGLFNTGARKTKLQMAFRNGTKST
                     YDIIKTRIKPGWITQEEELRMFKNWETIYREIRRAKLAEGLIQQLPIDTSKLHLSNYV
                     PTLHADHRNARSFNLPSATAVAPSTPASVQ"
BASE COUNT          636 a          719 c          804 g          622 t
ORIGIN      
        1 tgaaccttgg aagccaaaag taccccggcg cctcccttca gatcgccgaa ggtgctgaga
       61 ttgtaataag cgatgatccc tgccatacaa agaaggggca acagataggc cggcagatac
      121 ttgcgttgat ggaacagcat gaacagcgtc aaggcgccgg ccggaataaa attggtcatg
      181 cgacaaaata ctgccatgcc gagaaacaaa ccagccacgg ctgccagcaa attggagggc
      241 ctacactcct ggcggtacag tgcataggtt gcgatcgcca ggcacagagc acttgggccg
      301 tgttgccaga gtgcttggct ggagacggac catgcggatg ttcccagtcc gtaaaagaga
      361 gcaatcatcc aggcagccct ggtttcgatg gttaggctga gcgcaagaaa tataaataca
      421 gcggaaagcg caaccatcaa cgaagccgca atttttccgc taagccggta ttgataccat
      481 tgcttcaggt ggcctgagta ttttgcgatt gcgtaaaccg gcactgccat gataccggct
      541 cctggaggat agctggacac cagatgtccg ttacgttcct ggatactgtt gaaatatttc
      601 agacagatat ccagtgcgtt gtcgttgttg gcaagcaggg agcgagatct gtccaggttg
      661 aaagtacctt cggcaagaat gctggccgga agcacggtcg ccggaatcgt gtctggtgag
      721 tcgccaaagc ggaaattgag gttgtaaaaa cagaagagca gcaaggcgag cgcaagtgcc
      781 gcccagacag gagcgcaagg ttgttgaact ggcatctagg tttgcacgac ttccggcgat
      841 aagtcagagg aaaagtatat caccgccggg cagtactgaa agcgggctta ctgtacggaa
      901 gccggtgtgg atggagcaac ggcggttgca gaaggaaggt tgaagctacg tgcgttcctg
      961 tggtctgcgt gtagtgtggg cacatagttt gacaggtgca acttgctggt gtcgatggga
     1021 agttgctgga tgagaccttc ggccagtttg gcgcggcgga tctcgcgata gatcgtctcc
     1081 caatttttga acattcgcaa ctcctcctcc tgtgtgatcc agcccggctt gatccgtgtt
     1141 ttgatgatgt catacgtact tttcgtaccg ttgcgaaacg ccatttgcag cttggtttta
     1201 cgcgcgccgg tgttgaacag tcctttgagc gtccggagca gggcaaggag caatggcgca
     1261 cgctccgtgg agctgcgccg tgccccgtag aatctcagga aaatgcgcat cttcatcgct
     1321 gcctgagcga aacgactcaa gttttccgaa tagctttgtg ccggccagag catggcactg
     1381 tggaggatgt ggctgaaata ttcgcggttg atccggatct tgccagcatc atacagactg
     1441 tagaacagtt cggtccccgg cagcgccatg taaaagccga ccgaaacgtc ggtcaggccc
     1501 tcggcagcga gctgatcgag aaagggcatg ttctccatca gatgctcgcg cgtgtcgtgc
     1561 gggaagccca ccaccaggaa ggccactacg ttgagctttg ccgagaccgc cgcccggaga
     1621 ctttcaaaca gacgatccgt atgcattttt ttcttgatca acttgcgcgt cgtctccgat
     1681 cctgattcgg gggcgtaggc catgttgatc atgccggact tgaagagcag ttgcgccacc
     1741 tcttcgtcga ttgcttcggc gcgcgtgccg gttggcagtt gccaggtaat ctttagattg
     1801 cggcgcagga tttcctggca gaaggtgaca acccagtctt tcttaataat tgcagtcaga
     1861 tcctggagcg ggaaatttcc tgcgccgtat ttatggatgt acccctcgat ttcatcgacc
     1921 accttgaccg gatcacgtgg atcccagcgt ggggtccaca tgttcggtgc cgagcagtag
     1981 gtgcactgat atggacagcc acgcgtcgcc agaatgggta cgctgagcga tgccgagaaa
     2041 atgccaccga catagcgctg ctcgtgatag gtcttcaggt caaatagatc ccacgccgga
     2101 ctcgccagag tgtcgacttc ggtcatgcgt ttacgccgct ggttcacaat gatccggttg
     2161 tggtcgcgat cgcgaaacgc cagcccgtca atttcgtcaa gtggccgtcc cgtctccagg
     2221 gcatcggcga gttcgatgat ggtctcctca ccttcgccga gtaccaggat gtcggctttt
     2281 gaggtggaca gacagaactc cggcatcgcc gtgatgtgtt cgccgccaag aaccaccgtc
     2341 agatccggat gccgctgctt gatcagatcg atcagatgga cgatctccgg ccactcgtga
     2401 gtgaagatca cggaaatgcc gacgagcgag gcgtcagcag gaatgcgtgc cgccacttcg
     2461 gcgagtggga gtccaatcag gtagcccttg tagtagcgag tcatctctcc ggggctttct
     2521 gcgactgaat caatcaccgt taccctgcga cctgccgcct gcaacgcgcc agcgatgtag
     2581 gccaaaccaa gcggtggtga aatcttgaat gttgcagggg ctaaccgaaa gcactcgacc
     2641 gcgggtggtt tgatcaggca aatcatcttt tgattcatta ttctctccat ccccagatac
     2701 ctgttttcat tttatgccgc atataacagt gtggttaacc gtgaatagta aacaatccag
     2761 gctacatcga cggaagattg a
//
LOCUS       DAORVN010000243         2779 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_33728_length_2779_cov_2.859398, whole genome shotgun sequence.
ACCESSION   DAORVN010000243 DAORVN010000000
VERSION     DAORVN010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2779
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33728_length_2779_cov_2.859398"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /locus_tag="PKV23_05795"
     CDS             complement(<1..358)
                     /locus_tag="PKV23_05795"
                     /inference="COORDINATES: protein motif:HMM:NF018770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1365 family protein"
                     /protein_id="HQX84982.1"
                     /translation="MSGMQTCLYVGEVVHRRLAPVSHELRYRVYNFFADVDELPDLAR
                     RLKLFSYNRFNLFSIDDRKHGPGDGTPIHEFVWSLVRAAGKGDTVKRIFMFCYPRVLG
                     YVFNPLTVYYGVDASDR"
     gene            complement(355..1698)
                     /locus_tag="PKV23_05800"
     CDS             complement(355..1698)
                     /locus_tag="PKV23_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009757766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQX84983.1"
                     /translation="MNSHRIAVIGSGIAGLSAAWLLSQRHDVTLIEAEHRIGGHSNTV
                     NCLTPDGPIAVDTGFIVYNTATYPNLMALFDYLNVPTTPSLMGFGVSLDGGDCEYSGD
                     TLLQMMGNPGNLIVPGHWRMLWDIIRFFRTAEAQSKFLDEGVTLGQFLKAQGYSRAFM
                     ERHLLPMAGAIWSSSPNQMLDYPAVSFLRFLGNHGLLQYNDRPQWRTVTGGSREYVER
                     LVADGRFRILTGCPVRRVERSAAGVVVEDGLGFRDRFDHVVMATHADQALAMLKDPSP
                     EERQCLSAFRYGRNRAVLHRDAKLMPRRRRLWSSWNYMAERGAQGRSSSITYWMNALQ
                     PLATSTDIFVSLNPQREPEAALVEREFAYEHPIFTAEAGLMQKRLWSLQGRQRTWFCG
                     AHFGSGFHEDGLQSGLAVAEQLGGMLRPWHVPNQSGRIHVVPRAPAEPAHLEAAE"
     gene            1847..2458
                     /locus_tag="PKV23_05805"
     CDS             1847..2458
                     /locus_tag="PKV23_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007006767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HQX84984.1"
                     /translation="MTAVLAMSWNMLPKDNPADPKTEDLAAQRLVQRVARNLDREAFA
                     ELFDAFAPRVKTFIIRKGASPDLAEDLVQETMINVWTKAGYYDPAKGSVLTWIFTIAR
                     NLRIDRIRRESSRPVSELGDFDPPSEALAGEEILARKDEARSVARALSNIPPEQMEIL
                     TLSFIEDLPQAEIARRLNLPLGTVKSRMRLAYGHLRKNLEQPA"
     gene            2455..>2779
                     /locus_tag="PKV23_05810"
     CDS             2455..>2779
                     /locus_tag="PKV23_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018115952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX84985.1"
                     /translation="MNIEHHLDAATLVAYAAGTLDEAFNVVVASHLDWCPACAEEARR
                     AALLGGELIGKLAATPVSDECRSRTLGMLDTATLHRFPKAPSSGGELPEALEYLLKGK
                     SLAELS"
BASE COUNT          576 a          868 c          831 g          504 t
ORIGIN      
        1 gcctgtccga tgcatcaacg ccatagtaga ccgtcagggg attgaagaca tatcccagca
       61 cgcgcgggta gcagaacatg aagatgcgct tgaccgtgtc acccttgccg gcagccctga
      121 cgagtgacca gacaaattca tggatcggcg ttccgtcacc cgggccatgc ttgcggtcat
      181 caatgctgaa caggttgaaa cggttgtagc tgaaaagctt caggcgccgc gccaggtcag
      241 gcagctcatc gacatcggcg aagaaattgt aaacgcggta gcgcaattcg tggctgaccg
      301 gtgcaagacg ccggtgcacc acctcgccta catagaggca tgtctgcatc ccgctcattc
      361 ggcggcctca agatgcgcgg gctctgccgg agctcgcggc acgacatgaa tgcggccgga
      421 ctggttcggc acatgccacg gccgcaacat gccgcccagc tgttcggcaa ccgccagacc
      481 cgattgcagc ccatcctcgt ggaagccgga gccgaaatgc gccccgcaga accaggtgcg
      541 ctgccggccc tgaagcgacc acaggcgctt ctgcatgaga cccgcttcgg cagtaaagat
      601 gggatgctcg taagcgaact cgcgctcgac cagtgctgcc tccggctcac gctgcggatt
      661 gaggctgacg aaaatatccg tggacgtcgc cagtggctgc agggcattca tccagtaggt
      721 gatggaggag gaccggccct gcgcgcccct ctccgccatg tagttccagc ttgaccacag
      781 gcgccgccgc cgcggcataa gcttggcatc ccggtgcagc accgcccggt tgcgcccata
      841 gcgaaaagct gacaggcact ggcgttcttc cggtgatggg tccttcagca tggccagcgc
      901 ctggtccgca tgggttgcca tgacgacatg gtcgaagcgg tccctgaaac ccaggccgtc
      961 ctccaccacg acgccggcag ctgaacgctc gacacggcgc accggacagc ccgtcaggat
     1021 ccggaagcgg ccatcggcaa ccagcctctc cacgtattcg cgcgatcccc ccgtgaccgt
     1081 gcgccattgc ggccggtcat tgtactgaag caacccatga tttccgagga atctgagaaa
     1141 cgaaacggct gggtagtcca gcatctggtt tggcgaggat gaccagatgg ctccggccat
     1201 gggcagcaga tgcctttcca tgaaggcccg cgagtatccc tgggccttca ggaactggcc
     1261 gagggttacg ccttcatcga gaaatttaga ttgtgcttca gccgtccgga aaaaccggat
     1321 gatgtcccac agcatccgcc agtgcccggg cacgatgagg ttgccggggt ttcccatcat
     1381 ctgcagaagc gtgtcgcccg agtattcgca gtccccgcca tcaagcgaaa cgccgaaacc
     1441 catcagcgaa ggcgtggtcg gcacattgag gtagtcaaac agcgccatca ggtttggata
     1501 agtggcggtg ttgtagacaa taaagcccgt atccacggca atcggcccgt cgggtgtcaa
     1561 acagttaacc gtgttggaat gaccgccaat gcggtgctcg gcttcaatga gcgtcacgtc
     1621 atggcgttgc gacagcagcc aggcggcgga aagtcccgct attccagagc cgatgacggc
     1681 aattcggtgc gaattcaagc gattcaggcc ttcccatagg agtttacagt gaccctacgg
     1741 atgccggcgg aaagatggat caggtatcgg cacatttatt tgggaccggc ggcggcaaca
     1801 tgatctgaat tgatggtccc gccgtagggt tccgtgatag acagggatga ctgccgtgct
     1861 cgccatgagt tggaatatgc tgccaaaaga caacccggct gaccccaaga ctgaggatct
     1921 ggcggcccag cgtctggtgc agcgtgtggc caggaacctg gaccgtgagg ccttcgccga
     1981 actgttcgac gcctttgccc cgcgcgtgaa gaccttcatc atccgcaaag gcgcctcgcc
     2041 cgaccttgcc gaagacctgg tgcaggaaac catgatcaac gtctggacca aggctggcta
     2101 ctatgatccg gccaaaggct cggtcctcac ctggattttc accatcgcgc ggaacctccg
     2161 cattgaccgg atccggcgcg agtcgtcgcg gcccgtgtcg gaattgggcg atttcgatcc
     2221 gccatccgag gcgcttgccg gcgaggaaat tcttgcccgc aaggacgagg cccgcagcgt
     2281 ggcccgcgcc ctgagcaata ttccgcccga gcaaatggaa atcctcaccc tctcattcat
     2341 cgaggatctg ccgcaggctg aaatcgcccg gcgcctcaac ctgccgctcg gcaccgtcaa
     2401 atcgcgcatg cgcctcgcct atggccatct gcggaaaaac ctggagcaac ccgcgtgaac
     2461 attgaacatc atttggatgc ggcaaccctg gtcgcctacg cggcgggaac cctcgacgag
     2521 gcgttcaatg tcgtcgtggc gagccacctt gactggtgcc ccgcctgcgc tgaagaggcg
     2581 cgccgtgccg ccctgctggg cggggaactg atcggcaagc ttgcagcaac accggtgtcg
     2641 gatgaatgcc gcagcaggac actgggcatg ctcgataccg ccactctgca ccgctttccg
     2701 aaagcccctt catcgggggg agaactgccc gaggcgcttg aatatttgct gaaagggaaa
     2761 tctctcgctg agctttcct
//
LOCUS       DAORVN010000244         2772 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_33857_length_2772_cov_3.071034, whole genome shotgun sequence.
ACCESSION   DAORVN010000244 DAORVN010000000
VERSION     DAORVN010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2772)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2772)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2772
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33857_length_2772_cov_3.071034"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            3..1577
                     /locus_tag="PKV23_05815"
     CDS             3..1577
                     /locus_tag="PKV23_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006204252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HQX84986.1"
                     /translation="MIIGGGSAGCVLAARLSEMPEANVLLVEAGPRDTDPFIHMPVGF
                     FKMTGGPLTWGYETAPLRHANNRVAVYPQGRVLGGGSSINAEIYTRGCPEDYDGWASE
                     MNCKGWSWKDLKPYFVKSEGNTSLGGSEHGVDGPLGVTSPGSPNRVSQAYVQGCMDFG
                     MPHNADFNSGKLEGAGLYQTTIKDGKRCSAAVGYLRPAMKRPNLTVRTDLFVTRLLIE
                     KGRAIGVEVVSGSQLQRLEATSEVIVTAGAIGSPKLLMLSGIGPARHLREKDVSVIHD
                     LKGVGQNLHDHFSTDVTWVLNGPHSYDKYKKKHWQAWAGLQYLLYKSGPVASNIVEAG
                     AFWWSDRKEKIPDLQFHFLAGAGVEEGVGTVPGGNGATCNSYHTRPRSRGQVTLRSGN
                     PADAPHVDPNSFAEPYDLARHIDAIKITQEVGATRSMRRYVDHEHFPGPDCKTKKDYE
                     EVARSNARSSYHPVGTCKMGTDDMAVVDTKMRVRGIDALRVCDSSTMPRIVSTNTNAP
                     TIAMAERAADLIRGNR"
     gene            1578..>2772
                     /locus_tag="PKV23_05820"
     CDS             1578..>2772
                     /locus_tag="PKV23_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020813031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HQX84987.1"
                     /translation="MPRIVSAAEAVKQIADGATVTVNSSSGLCCPDAVLKALGERFDR
                     EKHPQNLTMLHPIAAGDMSGIKGVDHIAKPGLLSRIIGGSYPSGPSSAEPPLVWQMLG
                     ANQIAAYNVPSGIMFDMQREAAAKRPGVLTKVGLDTFVDPVHQGCAMNARAATAPIVK
                     KISFEGEEWLYFPAIIPNVAIIRATSADERGNLSYEHEGAYLGALDLAMAAHNNGGIV
                     IAQVKRVIRDRPLKPHDVYVPGILVDFIVEVPEQWQTTQTPYDPAISGEEFKPLDSFS
                     LMEFGTGKAIARRVSQELKEGWAVNLGFGVSANVPRIFLEEGLHDLAPHELLNQLYAA
                     LVARAGLDPATVGDVVLGCVTQHGEQAGNIAKTSALYAGWPASVSGITVNRFCSSGLQ
                     TIAMAA"
BASE COUNT          567 a          912 c          771 g          522 t
ORIGIN      
        1 acatcatcat cggcggcggc agcgcaggct gtgtccttgc cgccaggttg tctgaaatgc
       61 ccgaagccaa tgtgcttctg gtcgaggctg gtccccggga cacggatccc ttcatccata
      121 tgcccgtggg cttcttcaag atgaccggtg gccccctcac ctggggctat gagaccgcac
      181 ccctccgcca tgccaacaac cgcgtcgcgg tctatcccca gggccgcgta ctgggtggcg
      241 gcagttcgat caatgcggaa atttacactc gcggctgccc cgaggactac gacggctggg
      301 ccagcgaaat gaactgcaaa ggctggagct ggaaggatct caagccttat ttcgtgaagt
      361 cagagggcaa caccagcctg ggcggcagcg aacacggcgt tgacggcccg cttggcgtta
      421 ccagtcccgg cagtcccaac cgggtttccc aggcctatgt ccagggctgc atggacttcg
      481 gcatgcccca caatgctgac ttcaattcgg gcaagctgga aggcgccggc ctctatcaga
      541 ccaccatcaa ggacggcaaa cgctgcagcg cggcagtcgg ctatctcagg ccggccatga
      601 aacgccccaa ccttacggtg cgcaccgatc tctttgtcac ccgtctgctc attgaaaagg
      661 gccgcgccat aggcgttgaa gtcgtcagcg gcagccagtt gcagcggctg gaagccacaa
      721 gcgaagtcat tgtgacggcc ggcgccattg gctctcccaa actcctgatg ctgtccggca
      781 tcggcccagc aaggcatctc agggagaagg atgtatcagt catccatgat ctcaagggcg
      841 tcggccaaaa tctccacgac cacttctcaa ccgacgtcac ttgggtgctg aacggccccc
      901 actcctatga caagtataag aagaagcact ggcaagcctg ggccggcctg caatatctac
      961 tctacaaatc cgggccagtt gcgtccaaca tcgtggaagc cggggctttc tggtggagcg
     1021 accgcaaaga gaaaatcccc gatctgcaat ttcattttct ggccggcgct ggcgttgagg
     1081 aaggtgtggg caccgtcccc ggcggcaatg gtgccacctg caattcctat cacacccgcc
     1141 cccgttctcg cgggcaggtc acgctccgct ccggtaatcc cgccgatgcg ccgcatgttg
     1201 atcccaattc ctttgccgaa ccctatgatc tcgcccgcca cattgacgcg ataaaaataa
     1261 cccaggaagt gggcgccacc cgttccatgc ggcgctatgt ggaccatgaa cattttcccg
     1321 gccccgattg caaaacaaaa aaagattacg aagaagtggc ccgcagcaat gcccgctcct
     1381 cctatcatcc cgttggcacc tgcaagatgg gaactgatga tatggcggtg gtcgatacga
     1441 aaatgcgggt tcgcggcatt gacgcgcttc gtgtctgcga cagctccacc atgccgcgca
     1501 ttgtttccac caacaccaac gcacccacca ttgccatggc agaaagggct gcggacctga
     1561 tccgcggcaa cagataaatg cccagaattg tcagcgccgc cgaagccgtc aagcagatcg
     1621 ccgatggcgc caccgtcacc gtgaactcat cgtccggcct ctgctgcccc gatgcggtcc
     1681 tgaaggccct tggcgagcgc tttgaccgcg aaaagcatcc gcaaaacctc accatgctgc
     1741 atcccatagc ggctggcgac atgtcgggta tcaagggcgt agaccatatc gccaaaccgg
     1801 gcctgttgag ccgcatcatt ggcggttcct atccctccgg cccgtcatcg gcggaaccgc
     1861 cgctggtctg gcaaatgctc ggcgccaacc agatcgccgc ctacaacgtg ccctccggca
     1921 tcatgttcga tatgcagcgt gaagccgcag ccaaacgtcc cggcgtgctc accaaggttg
     1981 gccttgatac ctttgttgat cccgtccacc agggctgcgc catgaatgcc agggccgcca
     2041 ctgcgcccat agtgaagaaa atcagtttcg aaggcgagga gtggctttat ttccccgcca
     2101 tcattcccaa tgtcgccatc atccgcgcca cctcggcgga cgagcgcggc aacctctcct
     2161 atgagcatga gggcgcctat ctcggagcgc tcgatctagc catggccgcc cataacaacg
     2221 gcggaattgt catcgcccag gtaaagcgcg tgatccgcga ccggcccctg aagccccatg
     2281 atgtctacgt gccgggcatc ctggttgatt tcatcgtcga ggtcccggag caatggcaga
     2341 ccacccaaac accttacgat cccgccatct ccggcgagga attcaaaccg ctcgactcct
     2401 tcagcctcat ggaatttggc accggcaagg ccatcgctcg ccgcgtgtcc caggaactga
     2461 aagagggctg ggccgtaaat ctcggttttg gcgtatcggc caatgtcccg cgtatcttcc
     2521 tcgaggaagg cctccacgac ctcgcgccgc acgaactgct gaaccagctc tacgcggcgc
     2581 tggttgcacg cgccgggctg gatccggcaa cggtcggcga tgtggtgctt ggctgcgtca
     2641 cgcagcacgg cgagcaggcg ggcaatatcg ccaagacttc cgcgctttat gcgggctggc
     2701 cggcatccgt ttccggaatc acggtcaacc gtttctgttc gtcgggcctg cagaccatcg
     2761 ccatggccgc cc
//
LOCUS       DAORVN010000245         2767 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_33981_length_2767_cov_3.574853, whole genome shotgun sequence.
ACCESSION   DAORVN010000245 DAORVN010000000
VERSION     DAORVN010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2767
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33981_length_2767_cov_3.574853"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..142
                     /locus_tag="PKV23_05825"
     CDS             <1..142
                     /locus_tag="PKV23_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009758090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HQX84988.1"
                     /translation="CLPGSPGACRDAWDEILKYQLDYRHPPCNFVEIMPRLEEHQKRR
                     KG"
     gene            complement(139..1005)
                     /locus_tag="PKV23_05830"
     CDS             complement(139..1005)
                     /locus_tag="PKV23_05830"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HQX84989.1"
                     /translation="MNSKTLLLALVAPFCWGTGLAIAKPAVTHFPPMFMMLMVYFAIA
                     VITLITVHDRIKTPWRSLLLISAFSVTIQGAFVFWGLRGVEATTANLVLQTQVPMAVF
                     LGWLIAGEELNLRKIMGTAIALAGVGIVIGLPEQRPALLPVTLVILGGFFWAFGQTLA
                     RKLSQDSGIVLLKANALMGVPQLILATAIFETGQWHSITSAGTTEWATLAFVGFVGFY
                     LAYIAWFSLLRRCRMDDAAPFVLLMTPIGLLTAVIVLGERMSGAQLLGAAVLLLGLGI
                     VNGFLMPKQKLA"
     gene            1153..2220
                     /locus_tag="PKV23_05835"
     CDS             1153..2220
                     /locus_tag="PKV23_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017271364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HQX84990.1"
                     /translation="MALPQQMRGRGARNNDKGRYEKNKVSIFDDGWETLAELPPLKTT
                     IFEEQPKTIIARNQSPDISFDRSINPYRGCEHGCVYCYARPTHAYMGLSPGLDFESKL
                     FIKPNAAALLREELTAPNYLPRTMALGSNTDPYQPIERTYRITRQILEVLSEFNHPVG
                     IVTKSALVLRDLDILTSMAQRGLVKVAISVTTLDGKLARAMEPRASTPAKRLEALRVL
                     SAAGIPTVAMIGPVIPAVNDMEIEAILKAAAVAGVKEAGYTMLRLPLEVKDIFKEWLM
                     TEMPERASKVMGLVRSVRDGKENDAAFGRRMSGTGPYAWTIGRRFQLAAERLGINAKR
                     TTLATDLFQRPPQAGEQLTLI"
     gene            2241..>2767
                     /locus_tag="PKV23_05840"
     CDS             2241..>2767
                     /locus_tag="PKV23_05840"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HQX84991.1"
                     /translation="MAHDDSLGPDFSFELAAKAQGFSRVCGIDEAGRGPLAGPVVAAA
                     VILDPDNIPRGLNDSKKLDAETREALFAQIVKSAKIGVGIGDESRIDRDNILAATMWA
                     MAEAVQNLSDAPHYALVDGNRAPKLSCGVQTIVAGDARSLSIAAALIIAKVTRDRIMV
                     ALHDEFPGYGFARHKG"
BASE COUNT          591 a          801 c          861 g          514 t
ORIGIN      
        1 ctgcctgccg ggctcaccgg gtgcgtgcag ggatgcgtgg gatgaaatcc tgaagtatca
       61 gctggactac cggcatccgc cctgcaactt cgtcgaaatc atgccgcgcc tggaggagca
      121 tcagaagcgc cggaagggtt aagccagttt ctgcttcggc atgagaaagc cattgacgat
      181 gccgaggccc agcaacagca ccgccgcccc cagcagttgc gcgccggaca ttctctcgcc
      241 caatacgatg accgcggtga gaaggccgat gggcgtcatc agcagcacga acggggccgc
      301 gtcatccatg cggcagcggc ggagcagcga gaaccaggcg atataggcga ggtaaaagcc
      361 aacgaagccg acaaaggcga gggttgccca ttcggttgtc cctgccgagg tgatggagtg
      421 ccattggccc gtttcaaaaa tggcggtggc caggataagc tgcggcacgc ccatgagcgc
      481 attggccttg agcagcacga tgccgctgtc ctggctcagc ttgcgggcca gggtctgtcc
      541 aaaggcccag aagaacccgc ccaggataac gagtgtcaca ggcagcaggg caggacgctg
      601 ttccggcagg ccaatgacaa tcccgacgcc tgcaagcgct atggctgttc ccattatttt
      661 tcgcaggttc agttcctcac cggcaatgag ccagccgaga aagaccgcca tgggcacctg
      721 cgtctggagc acgagatttg cagtagtcgc ctcgacgccg cgcaggcccc agaacacgaa
      781 ggcaccctgg atggtgaccg aaaaggcgga gatcagtagc agggaacgcc acggggtttt
      841 tattctgtcg tgcacggtta tgagcgtgat cacggctatg gcgaagtaga ccataagcat
      901 catgaacatg ggcggaaaat gggtgacggc cggcttggcg atggccaatc cggttcccca
      961 gcagaagggg gcaacaaggg cgagtaacag ggttttggag ttcatgggcc agcaatggcg
     1021 ttattcgggc attatcacaa gtttgttctt gttatgttcc gacttttgcg ctacatatca
     1081 gccatgccca ctttgattga taacagaccc tccatctcct tcgatcccgc cgttacgggt
     1141 gccgggagac tcatggccct gccgcagcag atgcgcgggc gcggggcgcg caacaatgac
     1201 aagggccgtt acgaaaaaaa caaggtttcc atcttcgatg atgggtggga aacgctggcg
     1261 gaattgccgc cgctcaaaac taccattttt gaagagcagc ccaagaccat tatcgcgcgc
     1321 aaccagtcgc cggatatttc ctttgaccga tcaatcaatc cttaccgggg ctgcgagcat
     1381 ggctgcgtct actgctatgc caggccgacc cacgcctaca tggggctttc gccgggactc
     1441 gacttcgaga gcaagctttt catcaagccg aacgcggcgg cgcttctccg ggaggaatta
     1501 accgcgccca attacctgcc gcgcaccatg gcgcttggtt ccaatacaga tccctaccaa
     1561 ccaattgagc ggacttaccg catcacacgg caaatcctgg aagtgctgtc ggaattcaat
     1621 catcccgtcg gcattgtcac caagtcagcg ctggtgctgc gcgacctgga tattctcacg
     1681 tcaatggcgc agcgcggact ggtgaaggtc gcaatttccg tcacgacgct tgacgggaag
     1741 ctggcgcgcg ccatggagcc tcgagcctcg acccctgcca aacggctcga ggctcttcgc
     1801 gtgctttcgg cggcgggcat tcccaccgtg gcaatgatcg ggccggtgat tccggcggtg
     1861 aacgacatgg aaatcgaggc catcctgaaa gccgccgcgg tggcgggcgt gaaagaggcc
     1921 ggttacacca tgctgcggct gccgctcgag gtgaaagaca tcttcaagga atggctgatg
     1981 acggaaatgc cggagcgggc ttccaaggtc atggggctgg ttaggtccgt gagggacggc
     2041 aaggaaaatg acgcggcgtt cggcaggcgc atgagcggca ccgggcccta tgcgtggacc
     2101 atcgggcggc gctttcagct tgcggcggag cgcctcggca tcaatgcgaa acggacaacg
     2161 cttgctaccg atctgttcca aaggccgccg caggcgggtg aacagttgac gctgatatag
     2221 agttatggcc cacttgggcc atggcgcatg acgattccct tggaccggat ttcagttttg
     2281 aacttgcggc gaaagcccag gggttttcac gggtatgcgg catcgacgag gcggggcgcg
     2341 ggccgctggc cgggccggtg gtggccgcag ccgtcattct tgatcccgat aacattccgc
     2401 gcggcctgaa tgattccaaa aagctcgatg cggaaacacg cgaagcgctg tttgcccaaa
     2461 ttgtgaagtc ggcaaaaatc ggcgtgggca tcggcgatga aagccgcatc gaccgtgata
     2521 acattctcgc cgccaccatg tgggcaatgg cggaagcggt tcaaaacctc agcgacgcgc
     2581 cgcactatgc cctggtggac ggcaaccggg cgccgaaact ttcctgcggc gttcaaacca
     2641 ttgtggccgg tgatgcgcgc tcgctttcca tcgccgccgc cttgatcatc gccaaagtga
     2701 cgcgcgaccg gatcatggtg gcgctgcatg atgaattccc cggctacggc tttgcccgcc
     2761 acaaggg
//
LOCUS       DAORVN010000246         2761 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_34137_length_2761_cov_3.790835, whole genome shotgun sequence.
ACCESSION   DAORVN010000246 DAORVN010000000
VERSION     DAORVN010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2761)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2761)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2761
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34137_length_2761_cov_3.790835"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..437)
                     /locus_tag="PKV23_05845"
     CDS             complement(<1..437)
                     /locus_tag="PKV23_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008974698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase"
                     /protein_id="HQX84992.1"
                     /translation="MSAIEKQKETASFWFETLRDRIVAAFETIENEAPGTLPAGRFLQ
                     TPWTRENDGGGGVMSLLHGRLFEKVGVHCSTVHGEFSPEFRKQIPGAEDDPDFWASGI
                     SLIAHFWNPHVPAVHMNTRYVVTSKPWFGGVADLTPVLDRRRN"
     gene            complement(458..916)
                     /locus_tag="PKV23_05850"
     CDS             complement(458..916)
                     /locus_tag="PKV23_05850"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013167279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HQX84993.1"
                     /translation="MIEIALFQPDIAPNAATIIRMAACLGLKVTIVEPAGFAWTDSSF
                     KRAGLDYLDRAVVHRSPSWQAFRVQTRGQRLVLLSTKAALPYCQFGFTGTDILLMGQE
                     SAGVPPEVHEAVEARILIPMKPGERSLNVSLACAMVTGEALRQTAGFPVP"
     gene            1082..1669
                     /gene="petA"
                     /locus_tag="PKV23_05855"
     CDS             1082..1669
                     /gene="petA"
                     /locus_tag="PKV23_05855"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015316073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045275 - respiratory chain complex III
                     [Evidence IEA];
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HQX84994.1"
                     /translation="MSTAVHADVNRRDFLFIATGAVATVGAALTAWPFISQMNPDASV
                     LAMATIEVDLAPIAEGQEITLKWRGNPVFVRHRTAKEIELAKAVKLEELKDPVARSAN
                     VNDGEPATDENRVVGGKETFLVMMGVCTHLGCVPFGNQGEYGVVEGNTTSGGWFCPCH
                     GSHYDTAGRIRKGPAPENLFVPKYAYLSDTRILIG"
     gene            1685..>2761
                     /locus_tag="PKV23_05860"
     CDS             1685..>2761
                     /locus_tag="PKV23_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013167277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6"
                     /protein_id="HQX84995.1"
                     /translation="MSGPSNYQPKSAFGKWMHERLPVMELVQGQLMDFPTPKNLNYWW
                     TFGGILAIMLVAQLITGIVLAMHYTAHVDMAFDSVEHIMRDVNYGWLLRYLHANGASM
                     FFIAVYVHMFRGMYYGSYKAPREVLWMIGVLIYLVMMATAFMGYVLPWGQMSFWGAKV
                     ITNLFSAIPFVGESVTTWLWGGYSVDNPTLNRFFSLHYLLPFVLAGLVGLHVWALHVP
                     GNGNPTGVNVKGSQDTIPFHPYYTMKDGFAIVAFMILFAAFTFFAPNYLGHAINYLPA
                     NPLVTPAHIVPEWYYLPFYAILRAIPDKLLGVVAMFGSILILFVLPWLDTSRVRSGTY
                     RPIFKQFFWVFVLVCIGLGYLGSKP"
BASE COUNT          542 a          780 c          831 g          608 t
ORIGIN      
        1 tgattgcgcc tgcgatccag aaccggtgtc aggtccgcca cgccgccgaa ccagggtttg
       61 gaggtgacga catagcgggt gttcatgtgg accgccggaa cgtgggggtt ccagaaatgg
      121 gcaataagtg agattcccga agcccagaaa tccgggtcgt cttccgcacc cggaatctgc
      181 ttccggaact ccggagaaaa ctcgccgtgg acggttgagc agtgcacgcc cactttttca
      241 aacaacctgc cgtgaagcag ggacatgacg ccgccgccgc cgtcgttttc acgggtccat
      301 ggggtttgca ggaagcggcc ggcgggcagc gtgcccgggg cctcgttttc aatcgtctca
      361 aacgcggcaa caatccggtc cctgagggtt tcaaaccaga agctggccgt ttccttttgt
      421 ttttcaatgg cggacattgc ggctgatttc ttagggctca gggaacggga aagccagcgg
      481 tttggcgcag cgcttcgccc gtcaccatgg cgcaggcgag gctgacattg agggagcgct
      541 cacccggctt catgggaatc agaatgcggg cttccaccgc ctcgtggact tcgggcggca
      601 cgccggcact ttcctgcccc atcaggagga tgtcggttcc ggtgaaacca aactggcaat
      661 aggggagcgc cgccttggtt gacagcagga ccagcctctg cccccgggtt tgcacgcgga
      721 aggcttgcca ggatggagag cggtggacca cggcgcggtc cagatagtcc agcccggccc
      781 gcttgaaaga agaatcagtc caggcaaagc ctgccggttc gacaatcgtg actttcaggc
      841 caaggcaagc ggccatgcgg ataatggttg cggcattggg ggcaatgtcg ggctggaaaa
      901 gggcaatttc gatcatgatt ctggagtttt agccaatgtt gcggtgatga gcaaagcggc
      961 acggcgctgc gacaacttct ggctagacaa ccttcgccgc caatgccaaa accgccagag
     1021 tttatatgcc aaatgggggt ttcctccttg ggcgccagcg gcaacacgag ggaacaacaa
     1081 cgtgtccaca gcggtacatg cggatgtaaa tcggcgggat tttctattta ttgccaccgg
     1141 ggcggtggcg acagtcgggg cggccctcac ggcctggccc ttcatcagcc agatgaatcc
     1201 ggatgcctcg gttctggcaa tggcgaccat tgaagtcgat ctggcgccga tcgccgaggg
     1261 tcaggagatc accctgaagt ggcgcggaaa cccggttttt gtccgccacc gcacagccaa
     1321 ggaaatcgaa ctggccaagg cggtaaagct tgaagaactc aaggacccgg tcgcccgcag
     1381 cgccaatgtg aatgacggcg agccggcaac cgatgaaaac cgtgtcgtgg gcggcaagga
     1441 gacctttctg gtcatgatgg gggtttgcac ccacctcggc tgcgttccat tcggcaatca
     1501 gggcgagtac ggggtggtgg aaggcaacac gacttcgggc ggctggttct gcccctgcca
     1561 tggttcgcat tacgatacgg caggccgcat ccgcaaaggc ccggcgccgg agaatctttt
     1621 cgtgccaaaa tatgcgtatc tctccgacac aagaattctc atcggttaat tgggggcaag
     1681 attaatgagt ggtccgtcaa attatcaacc gaagagcgcc tttggcaaat ggatgcatga
     1741 gcggcttccg gtcatggaac tggttcaagg ccagttaatg gattttccga cgcccaagaa
     1801 cctcaactac tggtggacct tcggcggcat tctggccatc atgctggtgg cccagctcat
     1861 caccggcatc gtgctggcaa tgcattatac agcccatgtc gacatggcgt tcgactcggt
     1921 tgaacacatc atgcgcgatg tgaactacgg ctggctgctg cgctacctgc atgcaaatgg
     1981 cgcctcgatg ttcttcattg ccgtatatgt ccatatgttc cgcggcatgt actacggttc
     2041 ctacaaggcg ccgcgtgaag ttctgtggat gatcggcgtg ctgatctatc tcgtgatgat
     2101 ggccacggcg ttcatgggtt atgttctgcc ctggggccag atgagtttct ggggagccaa
     2161 ggtgatcacc aacctgttct cggcgatccc ctttgtaggc gagtcggtca cgacatggtt
     2221 gtggggcggc tactcggttg ataatcccac cttgaaccgc ttcttctcgc tgcactatct
     2281 cctgccgttt gttctggccg gccttgtggg gctgcatgtc tgggcgctgc atgtcccggg
     2341 caacggcaat cccaccggcg tcaacgtcaa gggctcgcag gataccattc cattccatcc
     2401 ctattatacg atgaaggatg gtttcgcgat cgtggccttc atgatcctgt tcgcagcatt
     2461 cacattcttc gcgccgaact acctgggcca tgccatcaac tacctgccgg ccaacccatt
     2521 ggtcacgccg gcgcatatcg ttccggaatg gtattacctg ccgttctacg cgatcctgcg
     2581 ggccattccc gacaagctgc ttggcgtcgt ggcgatgttc gggtcaatcc tgatcctgtt
     2641 cgtgctgccc tggctggata cttcacgggt gcgatcgggg acttaccggc ccattttcaa
     2701 gcagttcttc tgggtctttg tcctggtgtg catcgggctg ggttatctcg gttccaaacc
     2761 t
//
LOCUS       DAORVN010000247         2742 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_34545_length_2742_cov_4.028284, whole genome shotgun sequence.
ACCESSION   DAORVN010000247 DAORVN010000000
VERSION     DAORVN010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2742)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2742)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2742
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34545_length_2742_cov_4.028284"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..693)
                     /locus_tag="PKV23_05865"
     CDS             complement(<1..693)
                     /locus_tag="PKV23_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003498549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4167 domain-containing protein"
                     /protein_id="HQX84996.1"
                     /translation="MNFDNGHRARQGQHNKRGRGRNNRRPGGGGGSHNAGGGNSANRV
                     YESNGPDVKVRGTAQTVAEKYMQLGRDAQSSGDNVMAESYFQFSEHYFRVMAAAQPVG
                     QPTTQLRRPEDEDFEEEGGDAAEGETDTAQAAVVSESGQAPSGEQADSGETAGGNRQR
                     DNRDGNRERFRPRWQNRGDQPRDGDSGPRSAEAEVQMAQEPVPAGRPSGNVQETDSGQ
                     WEAPSFLRRPAPV"
     gene            complement(1012..1860)
                     /gene="prmC"
                     /locus_tag="PKV23_05870"
     CDS             complement(1012..1860)
                     /gene="prmC"
                     /locus_tag="PKV23_05870"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR03534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HQX84997.1"
                     /translation="MSETRRALLKQAGERLAASGIDTAALDARLLFQAASGLRHEDIV
                     AEPDLGVPRDVLTRFSEFIDRRCRCEPVSRILGTREFYGRSFRVTPDVLDPRADTETL
                     IEAALGLAQGREALRILDLGTGSGAIALTLLAELPWATAVASDLSAAALRVAKDNAEA
                     LGVAGRASFVQANWFAGIGGSFDLIVSNPPYIRLGDIAGLAADVREFDPLGALDGGPD
                     GLEAYRRISGGAGNNLALEGRVVLEIGAEQEDAVNELFTDQGFARESQHLDLSGLVRC
                     LVFRRA"
     gene            complement(1853..>2742)
                     /gene="prfA"
                     /locus_tag="PKV23_05875"
     CDS             complement(1853..>2742)
                     /gene="prfA"
                     /locus_tag="PKV23_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HQX84998.1"
                     /translation="EMLKAGGELAAMAEAEREALDAKIVELEHAIRILLLPRDAADER
                     NVILEVRAGTGGDEAAIFAGDLFRMYQRYAASKGWRVEVISASDGEHGGYREIIANIF
                     GAGAYARMKFESGVHRVQRVPDTETQGRIHTSAATVAVLPEAEEVDIKIEDKDLRIDV
                     YRSSGPGGQSVNTTDSAVRITHLPTGLAVAQQTEKSQLKNKNAAMKLLRSRLYDLERN
                     RIDSERSAQRKGQVGSGDRSERIRTYNFPQGRITDHRINLTLYKLDQIIEGPALEELI
                     LALMAEHQAELLATETSDV"
BASE COUNT          503 a          911 c          788 g          540 t
ORIGIN      
        1 aaccggcgcc ggccggcgca ggaatgaggg ggcctcccat tggccggaat cggtttcctg
       61 gacattgccg ctggggcggc ccgcagggac cggctcctgg gccatttgca cttccgcctc
      121 ggcggagcgc ggtccgctat cgccatcccg cggctgatcg ccccggttct gccagcgggg
      181 gcggaaacgc tcccggttgc catctcggtt gtcgcgctgg cggttgccgc cggctgtctc
      241 gccgctgtcg gcctgctcgc ccgaaggggc ttgcccgctt tccgaaacca ccgcggcctg
      301 cgcggtatcg gtctcgcctt ccgcggcatc gccgccctct tcctcaaaat cctcgtcttc
      361 cggccggcgc aactgcgtcg tcggctggcc caccggctgg gccgcggcca tcacccggaa
      421 ataatgttcg gagaactgga aatagctctc ggccatgaca ttgtcgccgg aagactgcgc
      481 gtcccgcccc agctgcatgt atttctcggc caccgtttgc gccgttccgc gcaccttgac
      541 gtccggcccg ttgctctcat agacgcggtt cgcggaattc ccgccgcccg cattatggct
      601 gccgcccccg ccgcccggtc tgcggttgtt gcggccgcgg ccgcgcttgt tgtgctggcc
      661 ctgacgtgca cggtgtccat tatcgaagtt caaatcatca ctgcggcaga atttgccgtc
      721 ccatattgtt gagattttgc attacaattt caaagatcgg ccctgatcaa agagtcgaaa
      781 taaccctaat acgatggttg tggtcgcaga cctctgccgc agatatttcc cctatcggaa
      841 acaccatccc cgactgcaaa aagcgagatg tgtggaactt gtgacctatg gtctttttcc
      901 caacatgccg cccaacgcca tcgcaagggc agaatgaatt accgactctt atgtagcgca
      961 gaaaaaagcg atttccaatg gtttttttag cttggccggc ccgcggcggt ttcaggcccg
     1021 cctgaacacc aggcagcgga caaggcccga caggtccaaa tgttggcttt cccgggcaaa
     1081 accctggtcc gtaaagagtt cattaaccgc atcttcctgt tccgccccga tctccagaac
     1141 aacgcggccc tccagagcca gattgttccc ggcgcccccc gaaatgcggc ggtaggcttc
     1201 cagaccgtca ggcccgccgt caagggcccc aagggggtcg aattcccgca catcggctgc
     1261 caaaccggcg atatccccaa gccggatata gggcggattc gacacgatga gatcaaagct
     1321 gccgccaatc cccgcaaacc agttcgcctg gacaaagctg gcccggcccg ccacgcccag
     1381 cgcttcggca ttgtccttcg caacccggag tgctgccgcc gaaagatcgc tggcaaccgc
     1441 ggtagcccac ggcaattcgg ccagcagggt caaagcaata gcgcccgagc cggtgccaag
     1501 gtcgagaatg cgcaaagctt cccggccctg cgccaggcca agcgccgctt cgatgagcgt
     1561 ttccgtgtcg gcgcgggggt ccaggacgtc aggcgtcacc cggaagcttc gcccatagaa
     1621 ttcacgggtt ccgaggatgc gcgaaaccgg ttcacatctg cagcgtcggt cgatgaattc
     1681 tgaaaaccgc gtcagaacat cgcgcggcac acccagatcc ggttccgcca cgatgtcctc
     1741 atgccgcaaa cctgaagccg cctgaaacag aagccgcgca tctagggctg ccgtgtcgat
     1801 gccgcttgcg gcaaggcgtt cgccagcttg ctttaaaagc gcccgccgtg tctcagacat
     1861 cagaggtttc agtggccaga agctccgcct gatgctccgc catgagggcg aggatcaatt
     1921 cctccaaagc cgggccctca atgatctggt cgagcttgta aagcgtgaga ttgatgcggt
     1981 ggtcggtaat ccgcccctgc ggaaaattat aggtgcggat gcgttcggaa cggtcgccgg
     2041 aacccacctg ccccttgcgc tgggccgaac gctcggaatc gatgcggttg cgctcgagat
     2101 cgtaaagcct ggaccgcaac agcttcatgg ccgcgttctt gttcttgagc tgcgattttt
     2161 ccgtctgctg ggcaaccgcc aatccggtgg gaagatgggt gatgcgcacc gccgaatccg
     2221 tggtgttcac cgactggccg ccagggcctg acgaacggta aacatcaatg cgcaaatcct
     2281 tgtcttcgat cttgatgtcc acctcttcag cctccggcaa gactgcaact gttgccgccg
     2341 aagtgtgaat gcgcccctgg gtttccgtat cgggcacgcg ctgtacccgg tgcacgccgg
     2401 attcaaactt catccgcgca taggccccgg ccccgaagat attggcgatg atttcgcggt
     2461 agcctccgtg ctcgccgtcg ctggccgaaa tgacttcaac ccgccagcct tttgaggccg
     2521 catagcgctg atacatgcga aacaggtcgc cggcgaaaat cgccgcctcg tcgccgcccg
     2581 tgccggcccg cacctcaaga atgacgttgc gctcatcggc ggcatcgcgc ggcaacagaa
     2641 ggatccggat cgcatgttcc agttccacga tcttggcgtc gagggcttca cgttccgctt
     2701 cggccatggc agccagttcg ccgccggcct tcagcatttc ct
//
LOCUS       DAORVN010000248         2727 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_34891_length_2727_cov_3.544162, whole genome shotgun sequence.
ACCESSION   DAORVN010000248 DAORVN010000000
VERSION     DAORVN010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2727
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34891_length_2727_cov_3.544162"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..254)
                     /locus_tag="PKV23_05880"
     CDS             complement(<1..254)
                     /locus_tag="PKV23_05880"
                     /inference="COORDINATES: protein motif:HMM:NF016852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LicD family protein"
                     /protein_id="HQX84999.1"
                     /translation="MQLADRIAGYIHELHRIRLPLTKRMVLPLNVKALERQLLDIHDA
                     FGHEGVRFWLRDGTALGLYRDGGIIPFDDDVDLGDWDADTP"
     gene            complement(437..748)
                     /locus_tag="PKV23_05885"
     CDS             complement(437..748)
                     /locus_tag="PKV23_05885"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HQX85000.1"
                     /translation="MVEAEQRRSRRRAVLMMASISHSTFSEDLVCKVREISQDGARLE
                     LPRARELPLAFFLRLDGETALRLCTTAWRSDTQVGVEFSQQILERNAESRNAVLNFHT
                     A"
     gene            complement(888..2216)
                     /locus_tag="PKV23_05890"
     CDS             complement(888..2216)
                     /locus_tag="PKV23_05890"
                     /inference="COORDINATES: protein motif:HMM:TIGR01843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HQX85001.1"
                     /translation="MKLSNWLKADGAAPGSNSFVLPLELEDARPVRIQRAVLRTGSVI
                     LLAATLWAGVTPIRELAIAPGQIVPSGDIRAVQHLEGGIIAEIYVNPGAMVQAGDPLL
                     RLAPDQTGGDLGQLQARSNDLRQRQKQLEQLIRTQKNGAPLRLSGLDLGAEQQAVLDT
                     RVNERLSEQRTLQSRIEQRHVEIANLEKEIKAFEGMVTIRQQVFDDKANLLKQGLATR
                     RDYFNDQSALEQAKTQLLNSRGKLASAREALDEANSLLAGSNAKAQLSWTEELSKVAA
                     DLKETDEALAKQKDRFSRLVVRAPVAGTVQMLTVKSAGEVVMPGDTFARIVPVDVPLV
                     AEVQVRPDDIGNVKIGDRTELRITAFDSSIYGKIHGKVASISPSSFQRENGDFYFKAA
                     VSLDETELPGHARVTPGMVVSAEIVIGAKSFLRYILKPIFRISGPAFSER"
     gene            complement(2251..>2727)
                     /locus_tag="PKV23_05895"
     CDS             complement(2251..>2727)
                     /locus_tag="PKV23_05895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85002.1"
                     /translation="GAAHCIGRDRVADQIALRLAAADSTDAELVRALEGVGISVSDPI
                     LSEGLETRLNAVNLRAMSLSFMQRLALARAFLSEAPIILLDEPANHLDREGDEALMRL
                     IAKVRGRSTVVMTTARPSHMRAADRVVVLHEGNLVAQGKPEQIVPRLMAQNTRAAG"
BASE COUNT          532 a          858 c          773 g          564 t
ORIGIN      
        1 ggggtgtcgg catcccagtc gccaagatct acatcgtcat caaacggtat gatgccacca
       61 tcccgatata accctagcgc agtaccgtcc ctcagccaga acctaacgcc ctcgtgccca
      121 aaagcatcgt ggatatccaa tagctgtcgt tcaagggcct tgacgttaag cggcaacacc
      181 atcctcttgg tgagcggtaa acgtatgcga tgcagctcgt gtatataacc cgcgatgcgg
      241 tcagcaagct gcacgtttat cacacgttgc cagggtatca tgtggcttat gggctcttca
      301 tgtgccgttt taccgccaaa aggacgaata ccttagacga gttgcactgt cgagcaacat
      361 ccgagctgct cccttgaggg aagcgacgcc agccaaccac catcaaaacg ccacgatcaa
      421 tcgttgcggc aacttactaa gctgtgtgga aattcagaac cgcgttgcgg ctttctgcgt
      481 tgcgttcgag aatctgctgg ctgaattcca ctcccacctg cgtgtccgag cgccaggccg
      541 tggtgcacag gcgaagggcg gtttcgccat cgaggcgcag aaaaaacgcc agcggcagtt
      601 ccctggcgcg cggcagttcc aagcgcgccc catcctggga gatttcacga accttgcaga
      661 ccaggtcctc actgaacgtg gaatgtgaaa tggaagccat cataaggaca gcgcgacgcc
      721 gggagcgtct ttgctcagct tcaaccatct cctttgccat ggattcacct gcccgcaacc
      781 tgcgatgctc cagtttatca gggcaatttt caaatttacc ttgtcaattt cggtaaaatt
      841 ttaggggttg agggtgccgg cctacgaggt aacccattga ttctatttca tcgctcgctg
      901 aaggccgggc ctgatatccg gaaaatcggc ttgaggatgt aacgcagaaa agacttggcg
      961 ccgatgacga tttcggcgga gacgaccatg cccggagtca ccctggcgtg gccgggaagc
     1021 tcggtctcgt cgaggctgac cgccgccttg aaataaaagt cgccgttttc gcgctgaaag
     1081 gaggatggcg aaatgctcgc gaccttgcca tggatcttgc cgtagattga ggaatcgaag
     1141 gccgtgatcc gcagttcggt ccggtcgcct atcttcacat ttccaatgtc atcgggcctg
     1201 acctggactt cggcaacgag gggcacgtcc acgggcacga tcctggcaaa ggtgtcacct
     1261 ggcatgacga cctcgcccgc ggactttacg gtcagcatct gcacggtgcc cgccaccggc
     1321 gcgcgcacga cgagccggct gaacctgtcc ttctgcttgg ccagcgcctc gtcggtttcc
     1381 ttgagatcgg cggcaacctt cgaaagctct tcagtccacg aaagctgggc cttcgcattg
     1441 gaccctgcca gcaggctgtt ggcttcgtcg agggcctccc tggctgacgc aagctttccc
     1501 cgggaattca ggagctgggt cttggcctgt tcgagtgccg actgatcatt gaaatagtcg
     1561 cgccttgttg ccaacccctg cttgagcagg ttggccttgt catcgaagac ctgctgccgg
     1621 atggtcacca tgccctcaaa ggccttgatt tccttttcca gattggcgat ctcgacatgg
     1681 cgctgctcga ttcgcgattg cagggtgcgc tgctcgctca ggcgctcgtt cacgcgcgta
     1741 tccagcacgg cctgctgctc ggccccgagg tcgaggccgc tcagcctgag cggcgcgccg
     1801 ttcttctgtg tcctgatcag ctgttcgagc tgcttctgcc gttgccgcag atcattcgag
     1861 cgggcctgca actggcccag gtcgccgcct gtctggtcag gggcaagccg caacaaggga
     1921 tcgcccgcct gcaccatggc gccggggttc acatagattt cagcaatgat cccgccctca
     1981 aggtgctgaa cggcgcggat atcgccgctt gggactatct gccccggcgc aatggccagt
     2041 tccctgatcg gcgtgacgcc cgcccagaga gtcgccgcca atagaatgac gctgccggtc
     2101 cgcagaaccg cccgctgaat ccggacggga cgcgcatcct caagttcaag cggcagaaca
     2161 aaggagttcg atcccggcgc cgcgccatca gcctttagcc agtttgaaag cttcatcttg
     2221 ttgcccctaa ctttacgtac cgcttcgtga tcatcccgca gccctcgtat tctgcgccat
     2281 caggcgcggc acaatctgtt caggcttgcc ctgcgccaca aggttgccct catgcaggac
     2341 aaccacccgg tcggcggcgc gcatgtggct cggccgtgcc gtcgtcatga caaccgtgct
     2401 gcggccacgg accttggcga tcaggcgcat cagcgcctca tcgccttccc ggtccaggtg
     2461 gtttgccggc tcatcaagca ggatgatcgg cgcttcgctc aggaaggcac gcgccaacgc
     2521 aaggcgctgc atgaatgaca gcgacatggc ccgcaggttc accgcgttca aacgggtttc
     2581 cagcccctcc gataaaatcg gatcggaaac gctgatgccc acgccctcga gggcgcgcac
     2641 cagttccgca tcggtggaat cggctgccgc aaggcgcagg gctatctgat cagccacccg
     2701 gtcccggccg atgcaatgcg cagcacc
//
LOCUS       DAORVN010000249         2723 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_34981_length_2723_cov_3.752624, whole genome shotgun sequence.
ACCESSION   DAORVN010000249 DAORVN010000000
VERSION     DAORVN010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2723
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34981_length_2723_cov_3.752624"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1195
                     /locus_tag="PKV23_05900"
     CDS             <1..1195
                     /locus_tag="PKV23_05900"
                     /inference="COORDINATES: protein motif:HMM:NF019371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HQX85003.1"
                     /translation="PGLRRLALRYLVPPRRPRFPSAPERLPIFQRRVASDDFSRVLPA
                     GANRAIDAVFLRSSCIRRYILFLTIAALATYTLSNNTRHASACKRTPAGFLLPMKFTK
                     PAISLDDQIALLRCRGLTIADEERARHYLRFVGYYRLAGYALPYQVNYNADGSHRFLD
                     GVSFEDILDLYVFDRKLRLAVMDAVERIEVAFRAQFSQTMSELHGPHWFMDTAHFIPS
                     YRHDKFIARIKDDIGHDTSRAAMRQTFIKHYYDEYGDPELPPSWMVFEVLSFGTVSLA
                     FKSLTRQNQKLVAKAFGLDGAVLASWLHALSYLRNLAAHHQRLWNRTYTIKPIQAKNY
                     ATELSDTTRFYAQAVMAEVLLKVISPDTHWAQRLADLLAEHPKVRADRLGFPADWRNR
                     PLWKP"
     gene            complement(1699..2022)
                     /locus_tag="PKV23_05905"
     CDS             complement(1699..2022)
                     /locus_tag="PKV23_05905"
                     /inference="COORDINATES: protein motif:HMM:TIGR02607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HQX85004.1"
                     /translation="MDFRDVTTGRHLAPVHPGNVLMKDFIEPLGITRYRVAKLAGVQQ
                     RRIDEICTGQRSITGDTALRLARLFSVDAAFWINLQAQYDLEVSYLEMHKRIEAEVTP
                     LAMAA"
     gene            complement(2065..2343)
                     /locus_tag="PKV23_05910"
                     /pseudo
     CDS             complement(2065..2343)
                     /locus_tag="PKV23_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561984.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2375..>2723)
                     /locus_tag="PKV23_05915"
     CDS             complement(2375..>2723)
                     /locus_tag="PKV23_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85005.1"
                     /translation="GRTGIAHVPGGSGRLQEPSIQHRKACTVAGIEGMTIHGLRRSFG
                     TLAEWVECPVGVAAQIMGHKPSATAEKHYRQRPLDLLRVWHTKIEAWILEQASIEFKP
                     KKAKPGLKVVTIA"
BASE COUNT          525 a          851 c          728 g          619 t
ORIGIN      
        1 gcccggcctg cggcgactcg cgctgcgcta cttggtcccg cctcggcggc ctcgcttccc
       61 atccgcccca gagaggctcc ccatcttcca gcgccgtgtc gccagcgacg acttctcccg
      121 tgtcttaccg gccggggcca atcgcgcaat tgatgcggtt tttctccgct catcctgcat
      181 tcgccgttac attctcttct tgaccatcgc cgcgctcgcc acatatactc tttccaacaa
      241 cacccgccac gcctctgctt gcaagcgcac cccggcgggt tttttattgc ccatgaaatt
      301 caccaagccc gccatttctc ttgacgacca gatcgcactg ctgcgctgtc gcgggctgac
      361 gattgccgac gaggagcgcg cccggcacta cctgcgtttt gtcggctact accggctggc
      421 gggttatgcg ctgccgtatc aggtgaatta caacgccgat ggttcccacc gcttcctcga
      481 cggcgtgagc tttgaagaca tccttgacct ttacgtgttc gaccgcaaac tgcggctggc
      541 ggtgatggat gccgtggagc gcatcgaggt ggcctttcgc gcccaattct cgcagaccat
      601 gagcgagttg cacggaccgc attggttcat ggataccgcc catttcatcc ccagctaccg
      661 ccacgacaag ttcatcgccc gcatcaagga cgacatcggc cacgatacgt cgcgggcagc
      721 gatgcgccaa accttcatca agcactacta cgacgagtac ggcgacccgg aactgccgcc
      781 gtcgtggatg gtgttcgagg tgctttcctt cggcacggtg tcgctggcct tcaaaagcct
      841 gacccgccag aaccagaagc tcgtcgccaa ggcgttcggc ctcgacggtg cggtgctcgc
      901 ctcctggctg cacgcgctgt cctatctgcg caacctggcg gcgcaccatc agcgcttgtg
      961 gaatcgcacc tacacgatca agccgatcca ggccaagaac tatgcgaccg aattgtcgga
     1021 cacgacccgc ttctatgccc aggccgtcat ggccgaagtg ttgctgaaag ttatttcacc
     1081 cgatacccat tgggcacagc ggctggccga tctgctggcc gagcatccca aggttcgagc
     1141 ggatcgcctc ggcttccccg ccgactggcg taatcgcccg ctctggaagc cgtagcgcgg
     1201 caataatcgc cgtgtcgcca gcgccgactt ttgcggcaga caagctgggg acggcgggtg
     1261 aatctccgcc gcgtctcgcc tgcaaaccct tgccaatact gggagcggtg aaaatccctg
     1321 tgtagttcct gtgtaatcgg gcatgggaaa tttcacaaaa ccccggaacc cggtggaaca
     1381 ccgccacgca ggccacgaca tctgaatcga ggaaattcat ggggttgcga agtttccaga
     1441 attcgctaga atgcgtttcc gttcccggcc tgaagactgt taatccgtag gtccctggtt
     1501 cgagcccagg tcgaggagcc agaattcaag cggcttacag cgatgtaagc cgcttttcat
     1561 ttgatggcac tgctaccgat ttgtagccta ccatcatccc atccaaccgc aaggctgcat
     1621 tcgccaggtg tcctggtgct acgtgagcat atcgttccat ttctgcaacg gcggcgccgc
     1681 cagggtgcgg tttgaactct atgcggccat cgccaagggc gtaacctcgg cctcgatgcg
     1741 cttgtgcatc tcaaggtaag aaacctcaag gtcatactgc gcttgcaggt tgatccagaa
     1801 ggcggcatcg acactaaata gccgggccag acgcagggca gtatcgccag taatcgagcg
     1861 ttggcctgtg catatctcat cgatgcgtcg ctgctgaaca cctgccagtt tggcgaccct
     1921 gtagcgggtg attcccagcg gttcgataaa gtccttcatc agaacattgc ccggatgcac
     1981 gggcgccaga tgacggccag tggtgacatc ccggaaatcc atatccttca gttgttcgcg
     2041 tgtaatagcc atgattctct cctggtgatc gtccgcaatc tcgacatccg ttgaatcacc
     2101 atcagcccag cggaagcaaa tgtgccattg atcgttgata cgaatgctcc attgcccggc
     2161 ccgatttcct ttcaacaatt ccagccggtt gcctggtggc acccgcaaac tctcgatgct
     2221 tgcggctgcg tcaatctgct tcaactttcg ccgcgctgta ttctgaatat cctttggcag
     2281 tttcttgact tgcaagccct cgaagattgt tgctgtttgc ttgtcggcga acgtctttat
     2341 catggttcag tagtattgcg aaacgatagt atcgtcaagc gatagtgacg acttttaatc
     2401 ccggtttggc tttcttgggt ttaaactcaa tgctggcctg ctcaagaatc catgcctcga
     2461 tctttgtatg ccagacccgc agcagatcaa gcggacgttg ccggtaatgc ttctcggctg
     2521 tggcgcttgg cttgtgtccc atgatctgtg cggccactcc caccggacat tcaacccatt
     2581 cagccagggt gccgaaagac cgacgaaggc catggatcgt cataccctcg atgccggcaa
     2641 cagtacaagc tttgcgatgc tggatgctcg gttcctgtag tcgtccggat ccaccgggaa
     2701 catgggcaat cccggtgcgg ccg
//
LOCUS       DAORVN010000250         2723 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_34990_length_2723_cov_2.778486, whole genome shotgun sequence.
ACCESSION   DAORVN010000250 DAORVN010000000
VERSION     DAORVN010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2723
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34990_length_2723_cov_2.778486"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..368
                     /locus_tag="PKV23_05920"
     CDS             <1..368
                     /locus_tag="PKV23_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018267570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6456 domain-containing protein"
                     /protein_id="HQX85006.1"
                     /translation="ERTSGRGFSTISDNRIAALSDGAIDARRRVHNAFDAVGPELSGV
                     LYYMCCLAGGLEQAERMLHLPQRSGKAVLAIALTRLARHYRLIKPPERSAASPGFAHW
                     ALADFRPRIMPPEPGARQP"
     gene            complement(334..750)
                     /locus_tag="PKV23_05925"
     CDS             complement(334..750)
                     /locus_tag="PKV23_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013165506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HQX85007.1"
                     /translation="MSTTTIENLQADFELLDDWEERYRYVIELGRLLPPFDEKFRNAQ
                     YKVSGCASQVWIHAEHNGAVLTFTGDSDALIVKGLIAITFMIFSGKTPEQILALDANA
                     IFAKLGLKDHLTPQRSNGLASMVARIKADARQVLAA"
     gene            complement(807..1292)
                     /locus_tag="PKV23_05930"
     CDS             complement(807..1292)
                     /locus_tag="PKV23_05930"
                     /inference="COORDINATES: protein motif:HMM:NF036461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5330 domain-containing protein"
                     /protein_id="HQX85008.1"
                     /translation="MFSLQISTIKGVDMRIVRTLIVLTGVAAFMPAPPEDVSQAAPQM
                     AGSAASDTGYFEVATRTFSDLASFCARQPGVCETAGFVAHKLELKAKYGARLIYEWAS
                     EASSTPPAVPPELAMGSDPIETATIKLAAAKRLPNNSQSTLRLDDIIPVWRDPGKAKT
                     G"
     gene            1484..2164
                     /locus_tag="PKV23_05935"
     CDS             1484..2164
                     /locus_tag="PKV23_05935"
                     /inference="COORDINATES: protein motif:HMM:NF013625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HQX85009.1"
                     /translation="MKDVLEEGGPTLRPLPVLALLATAVLSIAILYNAFLGQVDGAGR
                     RNFVASQETEGGASTRVDVMASNSRSATIVLKYDPRVEEVQRALLATGNYRGMVDGVA
                     GKNTRMAIAAYQKSAGLRVNGEVSNELIEHLRYTQKVAAASEFTASVERLERNPGEAA
                     ALRQVQTGLAELGYAPGEITGDLTSATIRAIIQFEKDRGLPRKGAVSSALLEEISKMS
                     GDSLIAAE"
     gene            2180..2518
                     /locus_tag="PKV23_05940"
     CDS             2180..2518
                     /locus_tag="PKV23_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015687394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1491 family protein"
                     /protein_id="HQX85010.1"
                     /translation="MRLKSEVWVQAFMRRCSVDGKYCTVASRGAAEAGAVFIVVNRLD
                     GRFHLFGPAPGPAFDGKGDRRFVEELPFPASEADVMTLLDRRKKFDSDLWIVEVEDRE
                     GTAGLSAAKI"
BASE COUNT          551 a          785 c          834 g          553 t
ORIGIN      
        1 gtgagcggac cagtggcagg ggcttcagca ccatcagcga caaccgcatc gccgcgctgt
       61 ccgacggcgc catcgatgcc cgaaggcggg ttcacaacgc ctttgatgcg gtgggaccgg
      121 agctttctgg cgtactctat tatatgtgct gcctcgcggg tgggctggag caggccgaac
      181 ggatgttgca tttgccccag cgcagcggca aggcggttct cgccattgcg ctgacccgcc
      241 tggcccgcca ctaccgcctg atcaagccgc cggagcgcag cgccgcaagc ccgggttttg
      301 cccactgggc cctggcggat ttccgcccgc ggattatgcc gccagaacct ggcgcgcgtc
      361 agccttgatg cgggccacca tggaggccag gccgttcgag cgctgcggcg tgaggtgatc
      421 cttcaggccc agcttggcga aaatcgcgtt ggcatcgagt gccaggatct gctcaggcgt
      481 ctttccggag aagatcatga aggtgatggc aatcagccct ttgacgatca gggcgtcact
      541 gtcgccggtg aaggtcagca ccgcgccatt gtgttcggcg tgaatccaca cctggctggc
      601 gcagccgctc actttatatt gggcattgcg gaatttctca tcgaaaggcg gcagcagccg
      661 gcccagctcg atgacatagc gatagcgttc ttcccaatcg tcgagaagct cgaaatcggc
      721 ctgcaggttt tcgatggtgg tggtgctcaa cgccagactc cgggagaaac gctctgtgcc
      781 gaagataggc gctggctatg cctaactcaa ccggtcttgg ctttgcccgg atcgcgccac
      841 accggaatga tgtcatcgag gcgaagcgtt gactgggaat tgtttggcag ccgcttggcc
      901 gcggccagct tgatggttgc cgtctcaatc ggatcgctgc ccatggcaag ctcgggtggc
      961 acggcgggcg gcgttgaact ggcttcgctg gcccattcat aaatcaggcg ggccccgtat
     1021 ttggccttga gttccagctt gtgggcgaca aatcccgccg tttcgcacac tccaggctgg
     1081 cgcgcgcaaa acgaggcgag atcggaaaat gtgcgggtag ccacctcaaa atagcccgta
     1141 tcggaagctg cgctgccggc catctgcggc gcagcctgag acacgtcttc aggcggcgct
     1201 ggcatgaaag ccgcgacgcc cgtcaaaacg atcagtgtcc gtacaatacg catatccaca
     1261 cccttgattg tggaaatttg tagcgaaaac atatttccgg cggttaaagc aaaagccgcg
     1321 gatttgctga gaatctgctg aaaatccgcc atttcgcggt aagactattt ttaccctgct
     1381 aagcaactga aagtgtgcgg gaatatggca gcaagacggt gttaaccagc gctgcggcaa
     1441 ggtggcggaa tcggggtgtt tgtatgggta tgggttacaa ttcatgaaag atgtgctgga
     1501 ggaaggcgga cctacactgc gcccgctccc tgtcctggcc ctccttgcca ccgcggttct
     1561 gagcattgcg atactttaca atgcattcct cggacaggtg gatggggccg gccgccgcaa
     1621 ctttgtggcc tcccaggaaa ctgaaggcgg cgcctcgacc cgcgttgatg tcatggcctc
     1681 caacagccgg agtgccacca ttgtcctgaa atatgacccg cgcgttgaag aggttcagcg
     1741 cgccctgctg gcaaccggca attaccgcgg catggtggac ggcgttgccg gcaagaacac
     1801 ccgcatggcc attgccgcct atcagaaatc cgcggggctt cgggtaaacg gcgaagtgtc
     1861 gaatgaactc atcgaacatc tccgctacac ccagaaggtt gccgccgctt cggaattcac
     1921 cgccagtgtt gagcggctgg aacgcaatcc gggcgaggcg gcggccctgc gccaggttca
     1981 gaccggcctg gccgaactcg gctatgctcc cggtgaaatt accggtgacc tcaccagcgc
     2041 caccatccgg gcgatcatcc agtttgaaaa ggaccgcggc ttgccgcgca aaggagcggt
     2101 ctccagcgcc ctgcttgagg agatttccaa gatgagcggt gattccctga ttgccgctga
     2161 atagcccgca gacagtttca tgcgattgaa atcagaagtc tgggttcagg ccttcatgcg
     2221 ccgctgcagc gttgatggaa aatactgcac cgttgcgagc cggggggcgg cagaagcagg
     2281 cgcggttttc attgtcgtta accgcctcga tggccgcttc catctctttg gcccggcgcc
     2341 gggacccgct ttcgacggga agggcgaccg gcgattcgtt gaagagctgc cgtttcccgc
     2401 aagcgaagct gacgtcatga ctctgttgga ccgccgcaaa aaatttgatt cggatctgtg
     2461 gattgttgaa gtggaggacc gcgaaggaac cgccgggctc tctgccgcaa aaatataaaa
     2521 tacggttagt caattcttaa ccaagtctcg ccttaactgc aaaaaagcgg aaacaacccg
     2581 cgaagagata agcgtctgcg aggaatggaa gaatgaatgt cgttttgcaa tttccggtaa
     2641 accgtgttgt gccgcgcagt cacgacgatg ccattctgtc cgccgcagtg gtgatttttc
     2701 ccggtgtccg ggtcgcacgg cgc
//
LOCUS       DAORVN010000251         2722 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_35004_length_2722_cov_3.103487, whole genome shotgun sequence.
ACCESSION   DAORVN010000251 DAORVN010000000
VERSION     DAORVN010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2722)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2722)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2722
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35004_length_2722_cov_3.103487"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1016)
                     /gene="sufD"
                     /locus_tag="PKV23_05945"
     CDS             complement(<1..1016)
                     /gene="sufD"
                     /locus_tag="PKV23_05945"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HQX85011.1"
                     /translation="MNVVVQKTAAEMNYGLSLPHRKMEDWRWTDLRQLVDKPYPPRQT
                     VVAAAKDVERLLRSSPFAKVGGARMVFVNGRYAAELSQLKNDTVTSTINVDEPVITMN
                     AAFATDGACLKISGTVDTPIELLFITTNAAARTIATRNVVEIEQGAAATLIETHLGEG
                     SYLSNSVTQIRVGEGARLDRIKVELEAANAIHLSHVHTTLGKNSVFNDFTLTSGAKTN
                     RQNGTVEFKGQGSEAKVSGAFMLGGRQHADTRLVIDHQVPHCTSRELFKCVMDDNARG
                     IFQGKVIVQRGAQKTDGKQSSHALLLSETAEFDAKPELEIFADDVVCGHGATSGDLNH
                     DHLF"
     gene            complement(1013..1768)
                     /gene="sufC"
                     /locus_tag="PKV23_05950"
     CDS             complement(1013..1768)
                     /gene="sufC"
                     /locus_tag="PKV23_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HQX85012.1"
                     /translation="MLEIKNLHVKLADEDRVILNGLTLSVKAGEVHAIMGPNGSGKST
                     LSYVLSGREGYEVTEGSVIYKGEDLLAMTPSERAAKGVFLAFQYPIEIPGVATMQFLK
                     VALNSQKKARGEKELSTPDFMRLVKERASKLNIGPDMLKRPVNVGFSGGEKKRAEILQ
                     MAVLEPSLCVLDETDSGLDIDALRVVADGVNGLRSPDRAMIVITHYQRLLDYIVPDHV
                     HVLSRGRIVKSGGKSLALELEAKGYAQFESEAA"
     gene            complement(1770..>2722)
                     /gene="sufB"
                     /locus_tag="PKV23_05955"
     CDS             complement(1770..>2722)
                     /gene="sufB"
                     /locus_tag="PKV23_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006614818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HQX85013.1"
                     /translation="KKYLGSVVPQGDNFYAALNAAVFSDGSFVYVPPNTRCPMELSTY
                     FRMNAKNTGQFERTLIIADKGSYVSYLEGCTAPMREESQLHAAVVELIALDDAEIKYS
                     TVQNWYPGDAEGKGGVYNFVTKRGDCRGRGAKISWTQVETGSAITWKYPSCILRGDNS
                     RGEFYSIAISNGRQQVDSGTKMIHLGKNTSSRIISKGIAAGRSDNTYRGLVSAHRKAT
                     NARNFTQCDSLLIGDKCGAHTVPYIEAKTLSAQFEHEATTSKISDDQMFYCMSRGLSQ
                     EEAVALIVNGFVRDVLQQLPMEFMAETQKLIGISLEGSVG"
BASE COUNT          526 a          714 c          859 g          623 t
ORIGIN      
        1 taaaaaagat gatcatggtt gaggtcaccc gaggtagcgc catggccgca gaccacatca
       61 tcggcgaaga tttcaagctc gggcttggca tcgaattcgg cggtttccga gagcaggagc
      121 gcatggctgg attgcttgcc gtcggttttc tgcgcgcccc tctgcacgat gaccttgccc
      181 tggaaaattc cgcgggcgtt gtcgtccatc acgcatttga aaagttcgcg gctggtgcag
      241 tggggcacct ggtggtcgat gaccaggcgt gtgtcggcgt gctgcctgcc gcccaacatg
      301 aaggcgccgg aaaccttggc ttcggacccc tgccctttga actccacggt gccgttctgg
      361 cgatttgttt tggcgccaga ggtcagggtg aagtcgttga acaccgagtt cttgccgagg
      421 gttgtatgga cgtggctgag gtggatggca ttggcggctt caagctcgac cttgatgcgg
      481 tcaagccttg cgccttcgcc gacgcgaatt tgggtgacgg aattgctgag gtaagagcct
      541 tcgccgagat gggtttcgat gagggtggcg gcggcgccct gttcgatttc aaccacattg
      601 cgggtggcga ttgtacgcgc agcggcgttc gtcgtaatga agagaagttc aatcggtgtg
      661 tcaaccgtgc cggaaatttt caggcatgca ccatcagttg caaaggcagc attcatggtg
      721 atcacgggtt catccacgtt tatagtggat gttacagtgt cattcttgag ttgcgagagt
      781 tcagcggcat agcggccatt gacgaaaacc atgcgcgcgc cgccgacctt ggcaaagggc
      841 gacgacctta gcaggcgttc aacatccttg gcagcagcaa cgacggtttg gcgtggcgga
      901 tagggcttat ccacgagctg gcgcagatcg gtccagcgcc agtcctccat cttgcgatgg
      961 ggcagcgaca ggccatagtt catttcggcc gcagtttttt ggacaaccac gttcacgccg
     1021 cctcgctttc aaattgggcg tagcccttgg cctcgagttc aagggccaga gacttgccgc
     1081 cggactttac gatgcggccc ctggacagga catgcacatg gtccggcaca atgtaatcaa
     1141 gcaggcgttg gtagtgggtg atgacgatca tggcgcggtc tggcgagcga aggccgttga
     1201 cgccgtcagc gacgactcgg agggcgtcga tgtcgaggcc tgaatccgtt tcatcgagca
     1261 cgcagagaga tggctcgagc accgccatct gaaggatttc tgcacgtttc ttttcaccgc
     1321 cggagaagcc gacattgacc gggcgtttga gcatgtccgg gccgatgttg agtttcgacg
     1381 cgcgctcctt caccaggcgc atgaagtcgg gggtggagag ttccttttcg cctcgggcct
     1441 ttttctggct attgagcgcc actttcaaga actgcatggt ggcaacgccg ggaatttcga
     1501 tggggtactg gaaagcgagg aatacgcctt tcgcggcgcg ttccgatggc gtcatggcaa
     1561 gcaggtcctc acccttgtag atgacggagc cttcggtgac ctcatagcct tcgcggcccg
     1621 atagcacata ggacagcgtg gacttgccag agccgttcgg ccccatgatg gcatggactt
     1681 cgccggcttt aaccgacagc gtaaggccgt tgaggatgac gcggtcctca tcggcgagct
     1741 tgacgtggag gtttttgatt tcgagcattt tatcctactg aaccttccag agatatacct
     1801 atgagctttt gtgtttccgc catgaattcc atgggcaatt gctgcagcac gtctcgcaca
     1861 aagccgttga cgatgagggc cacggcctct tcctggctga ggccgcgcga catgcagtag
     1921 aacatctggt catcggagat tttcgatgtg gtggcttcgt gctcgaactg ggcgctcaag
     1981 gttttggcct cgatgtaggg cacggtgtgg gcaccacact tgtcgccgat gagcagtgag
     2041 tcacattggg tgaagttgcg ggcgttagtc gccttgcggt gggccgagac gaggccgcga
     2101 taggtgttgt cggagcgtcc cgcggcaata ccctttgaga taatgcggct tgaggtgttc
     2161 ttgcccagat ggatcatctt ggtgccgctg tccacctgct ggcggccatt tgaaatggca
     2221 atggaataga attcgccgcg ggaattgtcg ccgcgcagga tgcaacttgg atacttccag
     2281 gtgatggcgg agcccgtctc aacctgggtc caggaaatct tggcccccct gccccggcaa
     2341 tcaccgcgct tggtgacgaa attgtagacg ccgcccttgc cttcggcatc gccgggatac
     2401 cagttctgca cggtggaata tttgatttcg gcatcatcta gggcaatgag ttcgaccacg
     2461 gcggcgtgaa gctggctttc ctctcgcatg ggcgcggtgc agccttcgag ataggaaacg
     2521 taggagccct tatcggcgat gatcagggtg cgctcgaact ggccggtgtt cttggcgttc
     2581 atgcggaaat aggttgagag ctccatgggg cagcgggtat tgggcggcac atagacaaat
     2641 gaaccatcgg aaaacactgc cgcgttaagg gccgcataga aattgtcacc ctgcggaacg
     2701 acggagccca gatatttctt ca
//
LOCUS       DAORVN010000252         2720 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_35052_length_2720_cov_3.557974, whole genome shotgun sequence.
ACCESSION   DAORVN010000252 DAORVN010000000
VERSION     DAORVN010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2720)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2720)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2720
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35052_length_2720_cov_3.557974"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..275)
                     /locus_tag="PKV23_05960"
     CDS             complement(<1..275)
                     /locus_tag="PKV23_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008872228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 associated
                     domain-containing protein"
                     /protein_id="HQX85014.1"
                     /translation="MTDSNDKNETKSGAGRAGGGTLTLKRPTTEQSRVKQSFSHGRTK
                     TVVVETKRKRFGDDKPASAEAKPMFQVQPKVAAPATPSVRRSSGTRAP"
     gene            complement(300..896)
                     /locus_tag="PKV23_05965"
     CDS             complement(300..896)
                     /locus_tag="PKV23_05965"
                     /inference="COORDINATES: protein motif:HMM:NF006622.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HQX85015.1"
                     /translation="MEAGLDEHLTERMCIVTRAVHDEDGLIRFVRSPDGYAVPDLQRK
                     LPGRGVWVTLSRERVSDAVRKNLFSRGFSAETKAAPELAETIGELLRKLALSYFSLAK
                     KAGLAVAGNSKVEDMVSKNLAQIIIHAVEAAPDGRRKIDKLAAPDVKVINIFHVDELD
                     LAFGRSNVIHAAVGRGRLAEKLLLAVQRIERYDAPLSA"
     gene            complement(910..2505)
                     /gene="nusA"
                     /locus_tag="PKV23_05970"
     CDS             complement(910..2505)
                     /gene="nusA"
                     /locus_tag="PKV23_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003598934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HQX85016.1"
                     /translation="MAEPVVSANRLELLQIADAVAREKVIDKSIVLQAMEEAITRAAK
                     ARYGAENEIRAEIDPKSGEIRLNRLLLVSEKVENEATEISLVEANRRNPAATVGDFIA
                     EPLPPIDFGRIAAQNAKQVIVQKVRDAERERMYDEFKDRIGQIVNGTVKRVEYGNVIV
                     DLGRGEAIVRRDEALPRETFRPGDRVRAYVFDVRREQRGPQIFLSRTHPEFMSKLFGQ
                     EVPEIYDGVVEVVSVSRDPGSRAKIAVRSKDGSIDPVGACVGMRGSRVQAVVNELQGE
                     KIDIIQWSPDVATFVVNALAPAEVAKVVLDEEAERIEVVVPDDQLSLAIGRRGQNVRL
                     ASQLTGWDIDIMTEAEESERRQKEFVARTARFIEALDVDETLAQLLASEGFDTVEEIA
                     YVDLNDIASIDGLDEQTGEELQNRAREYLDRVSKEQDARRVELGVSDELKELEGVSAA
                     MLVALGEAGIKTMEDLADCATDDLTGWTERKGTESVKHKGAFTGLEVSAAEAEALIMA
                     ARVKMGWIEAAAEPEEAVAEAEA"
     gene            complement(2498..>2720)
                     /locus_tag="PKV23_05975"
     CDS             complement(2498..>2720)
                     /locus_tag="PKV23_05975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85017.1"
                     /translation="DGEVRLFIDNPEGGKEPLLIGLPFADIGEAKLTLTDELIEAARA
                     RLPAKGLSDGSEWTEETTENSENGRMKNG"
BASE COUNT          484 a          920 c          708 g          608 t
ORIGIN      
        1 ggtgcccgcg taccactcga ccgtcgcacc gaaggggtgg cgggtgctgc cacctttggc
       61 tgcacctgaa acatcggctt tgcttccgct gacgcgggct tgtcatcgcc aaaacgcttg
      121 cgcttggttt ccacaacaac ggtcttggtc cgtccatgcg agaagctctg cttgaccctg
      181 ctctgctcag tcgtcgggcg cttgagggtc aaagttcccc cgccagcgcg ccccgcaccg
      241 gatttcgtct cgttcttgtc gttgctgtcg gtcatgctct ctctacaata acacaataac
      301 taggcgctca acggagcgtc atatcgttca atccgctgca cggccagaag cagtttttcg
      361 gcaagcctgc cccggcccac ggcagcatgt atcacatttg accggccaaa ggccaaatcc
      421 aattcatcaa cgtggaaaat attgatcact ttcacgtctg gcgccgccag cttgtcgatc
      481 ttgcgccgcc cgtctggtgc tgcttctaca gcatgtatga tgatttgcgc taggttttta
      541 gacaccatgt cctcaacctt gctgtttccg gccaccgcca gcccggcctt tttggccagg
      601 gaaaagtacg aaagggcgag tttccgcagc aattccccga ttgtctcggc aagctccggc
      661 gccgccttgg tttcggcgct aaacccccgg gaaaacaggt ttttcctgac cgcatcggaa
      721 acccgctcgc ggcttaacgt gacccaaacg ccccgccccg gcagcttgcg ctgcaggtcc
      781 ggaaccgcat agccatcggg gctgcgaaca aaacggataa ggccatcctc atcatgaacc
      841 gcgcgggtta caatgcacat gcgttccgtc aaatgttcgt ccagcccggc ttccatgctc
      901 ctcccagtct tatgcctcgg cttccgccac ggcctcttca ggttcagccg cggcctcgat
      961 ccagcccatc ttgacgcgcg ccgccatgat cagggcttcc gcctcggccg ccgaaacttc
     1021 cagacccgtg aacgcaccct tgtgtttgac gctctcggtg cccttgcgct cggtccagcc
     1081 cgtaaggtcg tctgtggcgc aatccgccag gtcttccatg gtcttgatgc ccgcttcgcc
     1141 aagcgccacc agcatggcgg cactcacccc ttcgagctct ttaagctcat cgctcacgcc
     1201 aagctcaact cgtcgtgcgt cctgctcttt cgaaacgcgg tccagatatt cccgcgcccg
     1261 gttctgcagt tcttcgccgg tctgctcgtc aaggccatcg atggaggcaa tgtcgttcag
     1321 gtcaacatag gcaatttctt ccaccgtatc gaagccttcc gacgccagaa gctgggccag
     1381 ggtttcatcc acgtcgagag cttcgatgaa gcgcgccgtg cgggccacga attccttctg
     1441 gcggcgctcg gattcctccg cttccgtcat gatgtcgatg tcccagcccg taagctgcga
     1501 agccagccgc acattctgtc cgcgccgccc aatcgccagt gacagttggt catccggcac
     1561 cacaacttca atacgctcgg cttcctcgtc gagaacaact ttcgcaactt ccgccggcgc
     1621 cagcgcgttc accacaaagg ttgccacatc cggcgaccac tggatgatgt cgatcttctc
     1681 gccctgcagc tcgttcacca ctgcctgaac gcggctgccg cgcatgccga cgcaggcgcc
     1741 gaccggatca atcgagccat ccttggagcg caccgcaatc ttggcgcggc tgccgggatc
     1801 gcgcgaaacc gagacgactt cgacaacgcc gtcgtagatt tccggcactt cctggccgaa
     1861 cagtttcgac atgaattccg gatgggtgcg gctgaggaat atctgcggcc cgcgctgctc
     1921 gcggcgcacg tcaaaaacgt aagccctaac ccggtcgccc gggcggaagg tttcccgcgg
     1981 caaggcttca tcgcgccgga caatcgcttc gccgcggccc agatcaacaa tcacattgcc
     2041 atattcaacc cgcttgaccg tgccgttgac gatctggccg atgcggtcct tgaactcgtc
     2101 atacatgcgc tcgcgttccg catccctgac cttctgcacg atcacctgct tggcgttctg
     2161 cgccgcaatg cgtccgaaat cgatgggcgg cagcggttcg gcaatgaagt cacccactgt
     2221 agcagcgggg ttgcggcggt tcgcttcaac caggctgatt tccgttgcct cgttctcgac
     2281 cttctcggaa accagcagca ggcggttcag gcggatttca ccggacttcg gatcaatctc
     2341 ggcgcggatt tcgttttcgg cgccataacg ggccttcgcc gcgcgcgtga tcgcttcttc
     2401 catggcctgc agcacgatgg atttgtcgat caccttttcg cgcgccaccg catcggcaat
     2461 ctgcaaaagc tcaagccggt ttgcacttac cactggttca gccatttttc attctcccgt
     2521 tttcggaatt ttccgttgtc tcttcagtcc attcgctgcc gtcactcaaa ccctttgcag
     2581 gcagtctggc gcgggccgct tcaatcagtt catcagtcag cgtgagcttc gcttcgccga
     2641 tatcggcaaa gggaaggccg atcagcagcg gttccttgcc cccttccggg ttatcaatga
     2701 aaagccgcac ttcgccgtcg
//
LOCUS       DAORVN010000253         2703 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_35464_length_2703_cov_2.646526, whole genome shotgun sequence.
ACCESSION   DAORVN010000253 DAORVN010000000
VERSION     DAORVN010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2703
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35464_length_2703_cov_2.646526"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(163..420)
                     /locus_tag="PKV23_05980"
     CDS             complement(163..420)
                     /locus_tag="PKV23_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007756862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4170 domain-containing protein"
                     /protein_id="HQX85018.1"
                     /translation="MKKPGLPKQLIHLVFGGELDDVKKVDFKDLSKLDVVGFFPNYAE
                     AHKAWLGKARETIDNAQMRYFIVHLHRLLDPATDTAKRKKS"
     gene            complement(436..1773)
                     /locus_tag="PKV23_05985"
     CDS             complement(436..1773)
                     /locus_tag="PKV23_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015669343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HQX85019.1"
                     /translation="MTDDLLTLAEDVLSRARKLGADSADALAVDAQGTDIEMRQGAIE
                     KLERTESRGVGLRVFVDHSSALISGSVFTPESLNRLAETAVAMARATPPDPLAGLAAP
                     ELLAVDFPQLDLVSSHLPESDELKALSLAVEGAALAVNGVSKSGGAGASASSRAIALV
                     TSAGFAQAYRRTGVSLSVSAIAGEGTGMERDHDYSAACHFEDLKTPQEVGKSAGERAV
                     KRLNARKIASQAVTVIYDRRVASSLLGHLSSAINGAAIARGTSFLKSKMGQQIFSKGI
                     SVIDDPLRLRGLASRPFDGEGLSSSRRELIADGILTGWVLDLRSARQLGLPPTGNGSR
                     GLSSPPGPSTSNLHLKPGQLSPEEMIGSLKQGLYITELIGNSVNLVTGDYSRGASGFW
                     IENGELAFPVSEITIAGNLGDMFMTLEPANDLIFKSSTNAPACRVEGLTIAGT"
     gene            complement(1770..2699)
                     /gene="ubiA"
                     /locus_tag="PKV23_05990"
     CDS             complement(1770..2699)
                     /gene="ubiA"
                     /locus_tag="PKV23_05990"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HQX85020.1"
                     /translation="MTETSHHIADAGRDNWVERLLPSTVQPYAQLMRLDRPIGWWLLL
                     LPCWWGLFLAQVSQGGGLPSLWFALLFLVGAIAMRGAGCTLNDIVDRNYDGRVARTKG
                     RPIPSGRLSVKQALIFLAAQALLGLAILLQFNSHTVVLAMTSLLIVAIYPFMKRITNW
                     PQLVLGLAFNWGVLVGWSAVHGSLSWPPVLVYLGGIFWTLAYDTIYAHQDREDDVLIG
                     VKSTAVHFGDNTRIWLGGFFILALISIDAALWLAGASFIAHMGVGFAALHAGWQIRKF
                     ETHNSALCLRLFKSNRDFGLIIAAFLFLDCLIP"
BASE COUNT          588 a          814 c          795 g          506 t
ORIGIN      
        1 tatcgatctc atccgtcccg accagaaatt caattcggtt gcggaactgg ttgatcagat
       61 gacgcaggat tgcatcgttg ccagggcccg gctcgaaagc ctgtcgcgtg gcaatccttg
      121 caccgcattt acccttggcc gcgcccagga agacggaaag atttaagact tcttgcgctt
      181 ggccgtgtcg gttgccggat cgagcagacg gtgcaggtgc acgatgaaat agcgcatctg
      241 ggcgttgtcg atggtctcgc gcgccttgcc aagccaggcc ttgtgggcct ctgcgtaatt
      301 cgggaaaaag ccgaccacgt ccagcttgga gaggtccttg aaatcaacct tcttgacgtc
      361 atcaagctcg ccgccaaaga cgaggtgaat cagttgtttc ggaagcccag gttttttcat
      421 ggtttcgctc ctagctcagg tgccggcaat ggtcaatcct tcgacccggc aggccggcgc
      481 atttgtcgat gacttgaaga tgagatcatt ggccggctcc agcgtcatga acatgtcgcc
      541 cagatttccg gcaatggtga tttcactgac cggaaaggcc agctctccgt tttcgatcca
      601 gaagcccgat gcgccccggc tgtagtcgcc ggtcaccagg ttgacgctgt tgccgatcag
      661 ttcagtgatg taaagcccct gtttgaggga accgatcatc tcctcgggac tgagctgtcc
      721 cggtttcaga tgaaggttgg acgtggaagg tccgggcggg ctgcttagtc cgcgcgaacc
      781 attgcccgtg ggcggaagtc caagctgccg cgccgagcgc aagtcgagca cccagcctgt
      841 gagaatgcca tcggcaatca gttctctcct gctggacgaa agtccttccc cgtcgaaggg
      901 ccgtgatgcc agcccgcgca agcgcagcgg atcatcaatg actgaaatgc ctttggaaaa
      961 aatctgctgg cccatcttgc tcttcaagaa gctcgtgccc cgggcgatgg cagcaccgtt
     1021 gatggccgaa gacaaatgcc ccagcaggct tgaggccacc cgcctgtcat agatcacggt
     1081 gaccgcctgc gaagcaatct tccgggcatt gagccgtttg accgcccgtt caccggcact
     1141 tttccccact tcctgtggcg tcttcaaatc ctcgaaatgg caggcggcgg aatagtcatg
     1201 gtcgcgctcc atgcccgtgc cttcaccggc gatggcggaa acgctgagcg agacgccggt
     1261 gcgccggtag gcctgggcga agcctgcgct ggtgaccagg gctattgcac gagacgacgc
     1321 cgatgcgccg gcccctcccg atttgctcac accgtttact gccagagccg caccttcaac
     1381 tgccagcgag agggccttga gttcatcgga ttccggcaga tgcgaggaaa ccagatcaag
     1441 ctgcggaaaa tcaaccgcca gcagttccgg ggcggccaga cctgcaagcg gatcaggcgg
     1501 tgtggcccgc gccatcgcca ccgccgtttc cgccaggcgg ttgagcgact ccggcgtaaa
     1561 cacgctgcct gaaatcagcg ccgaggaatg gtcgacaaaa actcttaaac caacaccacg
     1621 cgattccgtg cgttcaagct tttcaattgc gccctggcgc atttcaatgt cggtaccctg
     1681 cgcgtcaact gccagggcat cggcggaatc ggcgccaagt ttccgggcgc gcgataaaac
     1741 gtcttcggcg agcgtgagta ggtcgtcagt catgggatga ggcaatccag aaaaagaaaa
     1801 gcagcgatga taagtccaaa gtcgcggttg gatttgaaca ggcggaggca aagggcggaa
     1861 ttgtgtgttt caaattttct gatctgccac ccggcgtgaa gcgcggcaaa ccccactccc
     1921 atatgggcaa tgaacgaggc gcccgcaagc caaagcgcgg catcgattga aatcagcgcg
     1981 aggatgaaga aaccgccaag ccagattctg gtgttgtcgc cgaaatgcac cgctgtcgat
     2041 ttcaccccga tcagcacgtc gtcttcccgg tcctgatgcg catagatcgt gtcataggcc
     2101 agcgtccaga atatcccgcc gagataaacc aggaccggcg gccaggagag cgaaccgtga
     2161 accgcgctcc agcccaccag caccccccag ttgaaagcaa ggcccagaac cagctgcggc
     2221 cagttggtga tgcgcttcat gaagggatag attgcaacga tgagcagtga cgtcatcgcc
     2281 agcacgaccg tatgggaatt gaactgcagc aggatcgcaa ggccaagcag cgcctgcgct
     2341 gcgagaaaga tcaaagcctg tttcacgctc aaccggcccg aaggaatggg cctgcctttt
     2401 gtccgggcaa cgcgcccgtc ataattccgg tcgacaatgt cattgagcgt gcagcccgcg
     2461 ccgcgcatag cgatggcgcc caccagaaac agcaaggcaa accacaggct tggcaggccg
     2521 ccgccttgcg aaacctgcgc caggaacagg ccccaccagc acggcagcag caacagccac
     2581 cagccgatgg gccggtccag ccgcatgagc tgtgcatagg gctggacagt ggaaggcagc
     2641 aggcgctcca cccaattgtc gcggcccgca tcggcaatgt gatggctggt ctccgtcatg
     2701 tgc
//
LOCUS       DAORVN010000254         2693 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_35729_length_2693_cov_2.588324, whole genome shotgun sequence.
ACCESSION   DAORVN010000254 DAORVN010000000
VERSION     DAORVN010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2693
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35729_length_2693_cov_2.588324"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..155
                     /locus_tag="PKV23_05995"
     CDS             <1..155
                     /locus_tag="PKV23_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009026393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HQX85021.1"
                     /translation="LMPLYVCRRWTGVVTSLEGQELQWVKPLKLSDYPMPPADKPLIA
                     HLIDLL"
     gene            complement(275..1120)
                     /locus_tag="PKV23_06000"
     CDS             complement(275..1120)
                     /locus_tag="PKV23_06000"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HQX85022.1"
                     /translation="MTPGAEDIFDRHLYLARLERTKRGRPDILSHSIAAEFAERLDPI
                     NRVFDQACLIAADPEPIAAAIRASGKVTNLEVLEPNASEVLPLKGNAFDAIFDILNLH
                     LINDVPGRLIQLRRALIPDGLFMACLFAGQTLNELRQSWLEAEELVTGGVSPRVAPMI
                     DVREMGALLQRAELALPVVDLDRTMVRYPDAIALIHEIRSLGLSNVMTGRSRKPVTRN
                     LLGEAISIYQQKFSDDDDRIRATIEVAWATAWAPHESQQKPLKPGSAKIRLADALKVP
                     ETKLH"
     gene            complement(1127..1951)
                     /locus_tag="PKV23_06005"
     CDS             complement(1127..1951)
                     /locus_tag="PKV23_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009764174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HQX85023.1"
                     /translation="MAFARKDLERLADIVTIAADQEIMPRYRSLGEGAIREKTSALDL
                     VTDADEAAEAAIKAAIAKAFPKAVFIGEESVEKDKGLLNKISGADLAIIIDPVDGTSN
                     FAWGLPLFGVLAAVVERGVTVGGIIYDPVGRDCTFAIKGEGAWAKSESGAIRDIRVAK
                     PTRLANMTGTTSWYIMPEPLRSRVAANLPKVKASFGYRCAAYEYRIVAEGLVDFSLHY
                     KLMPWDHAAGVLIHAEAGGYTALLDGSPYLPTVFEGGVISAANRECWQMLHDALLR"
     gene            2005..>2693
                     /locus_tag="PKV23_06010"
     CDS             2005..>2693
                     /locus_tag="PKV23_06010"
                     /inference="COORDINATES: protein motif:HMM:NF039185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HQX85024.1"
                     /translation="MDEAAAQSTYSVRAWGRRFGRQVIDFLAPPKCLVCHDAVLEPAS
                     LCLSCWANLKLIDAPVCNVLGIPFAYDQGEGAISPAALAEPPTWDRARAAVAYDEASR
                     RIVHALKYRDTMEAGLLMARLMARAGQPLIGEADVIIPVPLHRFRLWGRRFNQAAFLS
                     QQISRQFGKAYRSDVLKRNRATRSQVGLSSDERRKNVAKVFQVMPEAVGHIAGRRVLL
                     VDDVLTTGATAG"
BASE COUNT          517 a          751 c          867 g          558 t
ORIGIN      
        1 tgctcatgcc gctctatgtt tgccggcggt ggacgggcgt ggtgacctcg ctcgaagggc
       61 aggagctgca atgggtgaaa ccgttgaagc tcagcgacta tcccatgcca ccggcggaca
      121 aaccgctcat cgcgcatctc atcgatctgc tttagcaccc taagtgttgc cagtctcatc
      181 atgcccgcac ttgatgcggg aatcttctcg gctgaacgag gaagggcgtg tccaacatgg
      241 ccatgatgag aagagaggtg ttcgcgaatc tcctttaatg cagcttcgtt tcgggcacct
      301 tcagggcatc ggccaggcgg atttttgccg aacccggctt caagggcttc tgctggcttt
      361 catggggagc ccaggcggtg gcccaagcca cttcaatggt ggcgcggatt cgatcgtcat
      421 catcggaaaa tttctgctga tagattgaaa tggcttcacc caggagattt cgggtgacgg
      481 gctttcgtga tcttccggtc atgacgttgg aaagaccgag cgacctgatc tcatggatga
      541 gggcgatggc atcggggtaa cgcaccatgg tgcggtcgag atccaccacg ggcagtgcga
      601 gttcggcgcg ctgcaggagc gcgcccattt cacggacatc gatcatgggg gcaacgcgtg
      661 gcgaaacgcc cccggtcacc agctcttcgg cttccagcca ggattggcgc aattcgttca
      721 gggtttgccc tgcgaataag caggccatga acaggccatc aggaatgagc gcgcggcgca
      781 actggataag tctacccggg acatcattga tgagatgcag attgaggata tcaaatatgg
      841 catcgaaagc attgcccttg agcggcagga cttccgatgc gttgggttca agcacctcga
      901 ggttggtgac tttgcccgag gcgcgaatag cggcggcaat cggctcaggg tcagccgcaa
      961 tcaggcaggc ctgatcgaac acgcgattta tggggtccaa tcgttccgcg aactcggcgg
     1021 ctatggaatg cgacaggata tccggacggc cgcgtttggt gcgctccaat cgtgcgagat
     1081 acaagtgacg gtcgaagata tcttctgcgc cgggcgtcat cggctatcag cggagcaagg
     1141 cgtcgtgaag catctgccag cattcgcgat ttgcggcgga gatgactccg ccttcaaaaa
     1201 ccgtcggcag ataggggctg ccatccagca gtgcggtata gccgccggcc tccgcatgga
     1261 tcagcacgcc cgccgcgtgg tcccaaggca tgagcttgta atgcagggaa aaatcaacca
     1321 gcccttcggc cacgatgcgg tattcataag cggcgcagcg atagccaaag ctggccttca
     1381 ctttgggaag gttggcggcg acacggctgc gcagcggttc cggcatgatg taccaggagg
     1441 ttgtgccggt catgttggca agccgggtcg gctttgccac gcggatatcg cggatggcgc
     1501 cgctttcgga tttggcccag gcgccttcgc cctttatggc aaaggtgcag tcgcggccca
     1561 cgggatcata gatgatgccg cccaccgtaa cgccacgttc aacaactgcc gccagcacgc
     1621 caaacagcgg caggccccag gcgaagttgc tggtgccgtc caccggatcg atgatgatgg
     1681 ccagatccgc cccggaaatt ttattcagaa ggcccttgtc tttttcgacc gattcttcgc
     1741 cgataaaaac ggctttggga aaggccttgg cgatggctgc cttgatggcc gcttcggcgg
     1801 cttcatcggc atcggtcacc agatcaaggg cagaggtttt ttcgcggatg gcgccttcgc
     1861 cgaggctgcg gtagcgcggc atgatttcct gatcggcggc gattgtcacg atatcggcca
     1921 gccgttccaa atcttttctt gcaaatgcca ttcttgttgt gctcctgatt gggcctgctt
     1981 tagatgaggt gtttgcaaaa agccatggac gaagctgccg cgcaatcgac gtattccgtc
     2041 cgggcatggg gcaggcgctt tggccggcag gtgatcgatt ttctggcacc gccgaaatgc
     2101 ctggtctgcc atgacgcggt gctggaaccg gcaagcctct gcctgtcctg ctgggcaaac
     2161 ctcaagctga tcgatgcgcc ggtctgcaat gtgctgggca ttccctttgc ctatgaccag
     2221 ggggaaggcg cgatcagccc ggcggcgctg gcggaacccc cgacctggga ccgggcgcgg
     2281 gcggcggtgg cctatgacga ggcgtcgcgg cggattgttc acgctctgaa ataccgggac
     2341 acgatggagg cgggattgct gatggcgcgg ctgatggcca gggccgggca gccgctcatc
     2401 ggtgaggccg atgtcattat ccccgtgcct ttgcaccgct tccgcctgtg gggcaggcgc
     2461 tttaaccagg cggccttcct gtcgcagcag atttcccggc agtttggcaa agcctatcgc
     2521 agtgacgtgc tcaagcggaa ccgggcaaca cgctcgcagg tgggcctgag ttctgacgag
     2581 cggcggaaga atgtggcgaa ggtcttccag gtgatgccgg aagcggttgg ccatattgcc
     2641 ggacggcgcg ttctgctggt tgacgatgtg ttaaccacgg gagcaacggc ggg
//
LOCUS       DAORVN010000255         2692 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_35752_length_2692_cov_2.791809, whole genome shotgun sequence.
ACCESSION   DAORVN010000255 DAORVN010000000
VERSION     DAORVN010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2692
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35752_length_2692_cov_2.791809"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..246
                     /locus_tag="PKV23_06015"
     CDS             <1..246
                     /locus_tag="PKV23_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008962376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding protein"
                     /protein_id="HQX85025.1"
                     /translation="LKKLVESGYVNHERSSADKRSVRVKLTPKGQAIRDRIDTLYNRQ
                     LQAVEEVVGLSIDEFDRLNKALTRLERFWTDQILYRL"
     gene            518..2311
                     /locus_tag="PKV23_06020"
     CDS             518..2311
                     /locus_tag="PKV23_06020"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HQX85026.1"
                     /translation="MFFSRVIGVSLAVILGASLINVPAPKAEPSEEMQALVKKRKLKS
                     MGILGSNAPAAHDAAPVVSGKAVTETEIFSTEVVAPMLSASGIADIQAAESKYAAIVS
                     NGGWPTVPKGALKKGSEGKGVVALNQRLFVEGYVRKEATEGEFASIFTSATEDGLKRF
                     QRNQGLRATGQIDGATLQALNVSAAARLSTIRANAPRIAEYSKDLGDRYVVVNVPAQQ
                     IETVSDNRVFSRHNAIVGRPSRPTPVVMTALSDINFNPYWNAPPSIVERDIVPKISSG
                     ILEKMNMKVFDGVGGPEVNARRINWRRTAVDNYHFRQEPGGDNAMATAKINFSSPFGI
                     YLHDTPEQHLFDTSNRFYSSGCVRVQKVAVLLDWILNGQDGIDPGQIDYLAQSLERKD
                     VKLAAPPQLRVAYLTAWPTSDGTVAFRSDVYGLDGTGFVVGQPLPVGEKTASGERFVL
                     KPVPRQLAQIDEAEADGFFWFGKRSRKDDSAADSRSPSSFAKKTKSASIFSRDADSKD
                     AKKKIIKKKVASTSKKKTTVAETKKPVAKPTTKVASATTKKAVKPAAPAAKKSNDLCK
                     PDKDGKLPKDCKVEAAAKPKEKPAETASATN"
     gene            2366..>2692
                     /locus_tag="PKV23_06025"
     CDS             2366..>2692
                     /locus_tag="PKV23_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012111828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HQX85027.1"
                     /translation="MTPQNYDRILAQGIDRLKAEGRYREFADICRVRGRFPRALYRGH
                     GAVREVTVWCSNDYLGMGQHPDVLAAMHAAVDDVGAGSGGTRNISGTTHYHVELEQEL
                     AGLHGKD"
BASE COUNT          610 a          774 c          774 g          534 t
ORIGIN      
        1 ttgaagaagc tggttgaatc cggctatgtg aaccatgaac gttcgagcgc cgacaagcgc
       61 tcggtccggg taaagctgac gccgaagggc caggccatcc gcgaccggat cgacacgctt
      121 tacaaccgtc agcttcaggc tgtcgaagag gtcgtcggcc tctcgatcga tgaattcgac
      181 cgcctgaaca aggcacttac ccggcttgag cgcttctgga ccgaccagat cctttatcgc
      241 ctctgaccgg atattccgca caatagccct gtggccgaaa agcctcaggg cctttttttg
      301 ttcaaaaacg ccctctcatt gccgtgttaa ggcggctttt gcctgatttt ggggggctta
      361 ccccgcctgt ggaatccagc gcaaaatttt gacgagtcgc gcccgggctg gtacatgtgg
      421 acaaatcagg aatgacacgc gcccaaggtc cgaaagagac ccggcgctgg ctggcaatga
      481 ggcaacaatg caattaactc taggctctgc gggcagattg tttttttcca gggtgattgg
      541 cgtttcactc gccgtgattc tgggcgcgtc gctcatcaat gttccggcgc caaaagccga
      601 gccatccgaa gaaatgcagg cactcgtcaa gaagcggaag ctcaagagca tgggcattct
      661 tggcagcaat gcgcctgcgg cccacgatgc agcacctgtt gtttcgggca aggccgtaac
      721 tgaaactgag atattctcaa ctgaagtcgt ggcgccaatg ctgtcggcaa gcggcatcgc
      781 cgacattcag gctgccgaaa gcaaatatgc ggcaatcgtc agcaacggcg gatggcccac
      841 cgtgccaaag ggcgcgttga agaaaggttc ggaaggcaaa ggggtagtgg ccctcaacca
      901 gcgcctgttc gttgaaggct atgtccggaa ggaagccact gagggcgaat ttgccagtat
      961 cttcacatcg gccacggaag atggcttgaa gcgcttccag cgcaatcaag gcttgcgggc
     1021 aacaggccag atcgatggcg cgacgctgca ggcgctcaat gtgtctgctg ccgcgcgcct
     1081 ctccacgatc cgggcgaatg cgccgcgcat agcggaatat tcaaaagacc tcggggaccg
     1141 ttacgtagtc gtgaatgttc ctgcccagca gatcgagacg gtgagcgaca accgggtgtt
     1201 ttcgcgccac aacgccattg ttggccggcc atcgcggcca acccctgtcg tgatgacggc
     1261 tctctcggac atcaatttca atccgtactg gaatgccccg ccgtcgatcg tcgagcgtga
     1321 tattgttccc aagatttcca gcggcatcct ggaaaagatg aacatgaagg tctttgacgg
     1381 ggtcggcggg cctgaagtca atgccaggcg catcaactgg cgccgcaccg ccgttgacaa
     1441 ttatcatttc cggcaggaac ccggcggtga caacgcaatg gccaccgcca agatcaattt
     1501 ttccagcccc ttcgggattt acctccacga tacccccgag cagcatctct ttgataccag
     1561 caaccgtttt tacagctcgg gttgcgtgcg ggttcagaaa gttgccgtac ttcttgactg
     1621 gattttgaac ggccaggacg ggatagatcc cggccagatt gactatctcg cccaatcgct
     1681 tgagcgcaag gatgtgaagc tcgctgcgcc gccccaattg cgcgttgcct atctcaccgc
     1741 ctggcccacg tcggatggaa cagtggcgtt ccgcagtgat gtctatggtt tggatggaac
     1801 cgggtttgtt gtgggccagc cattgccggt tggcgaaaag accgcaagcg gcgagcgctt
     1861 cgtgctgaag cccgttcccc gccagcttgc gcagatcgac gaagccgaag ccgacggatt
     1921 tttctggttc ggcaagcggt cgagaaagga tgacagcgct gcagattctc gctcgcccag
     1981 ttcctttgcg aagaaaacga agtctgcgag catcttcagc cgtgacgccg actccaagga
     2041 cgccaagaaa aaaatcataa agaaaaaagt cgcttcaacc tctaagaaga agacaacggt
     2101 tgctgaaacc aagaagccgg tggcaaagcc cacgaccaaa gttgcctctg caaccaccaa
     2161 aaaggcggtc aagccggcag cacccgcagc caagaaatcc aacgacctgt gcaagcccga
     2221 caaggatggc aagctgccaa aggattgcaa ggtggaagcg gctgcgaaac cgaaggaaaa
     2281 gccggcagag acggcctctg cgaccaactg accctgcgtt tttttgcgcg gtaacctggc
     2341 ggccttgaat ggctaagatt tgcttatgac tccgcaaaac tatgaccgca ttcttgcaca
     2401 gggcattgac cgcctgaaag ccgagggccg ctaccgcgaa ttcgccgata tttgccgtgt
     2461 ccggggcagg tttccccgtg cgctctatcg cgggcacggt gcggtgcgtg aagtgaccgt
     2521 gtggtgctcc aatgattatc tcggaatggg ccagcatccc gatgttcttg ccgccatgca
     2581 cgcagccgtt gacgacgtgg gcgcgggctc cggcggcacc cgcaacattt ccggcaccac
     2641 ccattatcat gtcgagcttg aacaggaact ggccggcctc catggcaagg ac
//
LOCUS       DAORVN010000256         2685 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_35916_length_2685_cov_3.153612, whole genome shotgun sequence.
ACCESSION   DAORVN010000256 DAORVN010000000
VERSION     DAORVN010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2685
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35916_length_2685_cov_3.153612"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..362
                     /locus_tag="PKV23_06030"
     CDS             <1..362
                     /locus_tag="PKV23_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010911030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HQX85028.1"
                     /translation="AKPTEIMFFQSLLLAIFFLLPAPWLAVSLAQEHWLALVGVTALS
                     LSGQFLMSWSYARAEAQYLIPTEYSAFVWAILFGWFFFNEGVVWTTLVGAGLIIAGCL
                     IAARSNARLAEPIEATV"
     gene            complement(374..1399)
                     /locus_tag="PKV23_06035"
     CDS             complement(374..1399)
                     /locus_tag="PKV23_06035"
                     /inference="COORDINATES: protein motif:HMM:NF013215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IB"
                     /protein_id="HQX85029.1"
                     /translation="MSSLGDSETIRESAIEFHGIRRITANDLGIKRSKNGASYSYIDE
                     PKRTISRTHLKRIAELVIPPAWSDVRIATEKDAHLQAVGRDAAGRLQYIYHPEWVEVR
                     AAAKAFRLSQLGSVLGRLRVCIRQDLRERSARMPLAAAARIVDLLHLRAGHEAYAGDE
                     GGRGVCTLLKRHLKMDSDGFHLVFRGKGGKHIEKSCDDPLLIAALKELRAIPGVRLFK
                     LKTNDGYRPMTAGDLNQYLAESSGKPITAKDFRTLFASTVALNQLQNFGPPATTAAAR
                     RAIMDAARKISTELANTPAVTRKSYINPMILERFEKGELENPADFPKKRGLTLAESRL
                     MQFLKTT"
     gene            complement(1550..>2685)
                     /locus_tag="PKV23_06040"
     CDS             complement(1550..>2685)
                     /locus_tag="PKV23_06040"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HQX85030.1"
                     /translation="VEGLAVAVEKAVVHCGDNPNVLFRRAQLANRKAEFEIAADYLNE
                     VQADRLQPTLQAAYFGLRGKTCDRLGQFDQAFSAFETQNALTGSSADARINANGYLNS
                     IQARKKSWSGDAEPNWKRSSTEPGRATPAFMIGFPRSGTTLLDTILRSHPGISVVEEK
                     PMVAEMDKALQQAHTIETLNTLTDVEVNGLRDAYFMELKLHLGEGHDGKLIIDKLPLN
                     ITRAGLIHRVFPDAKFILVLRHPCDCVLSCFMQSFKLNDAMANFLGLEQSARLYGAVM
                     ELWSAYRQKLDIDVHVVKYEDLIQDFEGTCNGLLRFLGLAWDDNLRNYQKTALDRSSI
                     HTPSYSQVVQPLYTQASGRWTNYRKQMEPVLPVLEPWITSFGY"
BASE COUNT          505 a          793 c          783 g          604 t
ORIGIN      
        1 tggccaagcc caccgagatc atgttctttc aaagcctgct gctcgccata ttttttcttc
       61 tgcccgcgcc ctggctggcc gtgagcctgg cgcaggagca ctggctggcg ctggtgggtg
      121 tcaccgccct gtcgctgagc ggccagttcc tcatgagctg gtcctacgcc cgcgccgaag
      181 cgcagtattt gatccccacg gaatattccg ccttcgtctg ggcaattctg tttggctggt
      241 tcttcttcaa tgaaggtgtc gtgtggacca ccctggtggg cgccggcctg atcatcgccg
      301 gctgcctgat tgccgcgcgt tcaaacgcca ggcttgcgga acccatcgaa gcgacggttt
      361 agagagcttg atcttaagtg gtcttgagaa actgcatgag tcttgactcg gcaagggtca
      421 gcccgcgctt ctttggaaaa tccgcgggat tttcaagttc gcccttttcg aatcgctcga
      481 ggatcatcgg attgatgtaa ctctttcggg tgaccgcggg cgtgttggca agttccgtac
      541 tgattttccg cgccgcatcc atgatggcgc gccgtgccgc agccgttgtc gccggcggcc
      601 cgaagttctg cagctgattg agcgccaccg tggaggcaaa gagcgtgcgg aaatcttttg
      661 ccgtgatggg cttgccggaa ctttcggcca ggtattgatt gaggtcaccc gccgtcatcg
      721 gccggtaccc gtcattggtt ttcagtttga agagcctgac gcccggaatg gcgcgcaatt
      781 ctttcagggc ggcgatgagc aggggatcgt cacagctttt ctcgatgtgc tttccgccct
      841 tgccgcggaa caccagatga aagccgtcac tgtccatctt gaggtggcgc ttgagcaggg
      901 tgcagacgcc gcgcccgcct tcatcgccgg cataggcttc atggccggcg cgcaagtgca
      961 gaagatcaac gatccgcgcc gcggcggcca agggcatgcg cgctgaacgt tcgcgcaggt
     1021 cctgccttat gcagacgcgc agccggccga gcacgcttcc caactgggaa agccggaaag
     1081 ccttggcggc agcccgcacc tccacccatt ccggatggta gatatattgc aggcgacccg
     1141 ccgcatcccg tcccaccgcc tggagatggg cgtccttttc ggtggcgatc cggacatccg
     1201 accacgccgg cgggatgacg agctcggcta ttcttttcag atgggtccgt gaaatggtcc
     1261 gcttgggttc atcgatatag gagtagctgg cgccattctt cgaacgcttg atgcccaggt
     1321 cattggccgt gatgcggcgg attccatgga attcgatggc gctttcccgg atcgtctctg
     1381 aatcacctag acttgacaca acttacccct ctggccccat ggccgatccg gcaacttaca
     1441 aataagagct aaatgaaagg ttccaagccc tgccacctcc cgggtggcat ggttaacaga
     1501 atacagctct gcaggggttt ttggccttta ccggcacccg ggccagacat cagtacccaa
     1561 acgacgtgat ccaaggctcc agcacaggca gcacgggctc catctgcttg cggtagtttg
     1621 tccaccgccc gctggcctgc gtgtacaggg gctggacgac ctggctgtag ctgggggtgt
     1681 ggatgctgct cctgtccagc gcggttttct gataattgcg caaattgtca tcccaagcga
     1741 ggccgagaaa ccggagcagg ccattgcatg tgccctcaaa atcttgaatg agatcttcat
     1801 acttcacgac atgcacgtcg atatcgagtt tttgccggta agcggaccac agttccatga
     1861 ccgcgccata gagccttgcc gattgctcaa ggcccaggaa attggccatg gcgtcattga
     1921 gtttgaaact ctgcatgaag cagctcagca cgcagtcgca ggggtggcgc aagacaagga
     1981 tgaatttcgc atccggaaac acccggtgga tgaggccggc acgggtgata ttcaatggca
     2041 gcttatcgat gataagcttg ccgtcgtgac cctcgccaag atgcagcttc aattccatga
     2101 agtaggcgtc gcgcaggccg ttgacctcta cgtccgtaag cgtgttgagc gtttcaatag
     2161 tgtgtgcctg ctgcagggcc ttgtccattt cggcgaccat gggtttttcc tcaaccacgc
     2221 tgatccccgg gtggctgcgc agaattgtat cgagcagcgt cgtccccgat ctcggaaagc
     2281 cgatcatgaa tgcgggcgtg gcccggcccg gctccgtcga agaccgtttc cagttcggct
     2341 ccgcatcgcc gctccacgat ttttttcgcg cttgaattga attcagatag ccatttgcat
     2401 tgatccgggc atctgctgaa gacccggtca acgcgttctg cgtctcaaaa gccgaaaagg
     2461 cctgatcaaa ttggccgagc cggtcgcagg tcttgccgcg aaggccgaaa tacgccgcct
     2521 gcagggtggg ttgcagcctg tctgcttgaa cctcattaag atagtccgcg gcaatctcaa
     2581 actctgcctt gcggtttgcc agctgggcgc ggcggaagag gacattcgga ttgtccccgc
     2641 aatggaccac cgctttttcc acggccaccg cgagcccctc aacgt
//
LOCUS       DAORVN010000257         2684 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_35946_length_2684_cov_2.791175, whole genome shotgun sequence.
ACCESSION   DAORVN010000257 DAORVN010000000
VERSION     DAORVN010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2684)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2684)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2684
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35946_length_2684_cov_2.791175"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="PKV23_06045"
     CDS             complement(<1..194)
                     /locus_tag="PKV23_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009757492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HQX85031.1"
                     /translation="MFDLTGKTALITGATGGIGAGIAKALHGAGATIAVSGTRAAVLE
                     ELKAALGGRVHVLPCNLSDPA"
     gene            complement(199..1149)
                     /gene="fabD"
                     /locus_tag="PKV23_06050"
     CDS             complement(199..1149)
                     /gene="fabD"
                     /locus_tag="PKV23_06050"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HQX85032.1"
                     /translation="MSIAFTFPGQGSQAVGMGKALADAFPAARTVFDEVDAALSQKLS
                     TLMWEGPEADLTLTANAQPALMAVSMAVLAVLQAEHGVTVASTAKFVGGHSLGEYSAL
                     AAAGTFSLSDTARLLRIRGKAMQAATPLGIGAMAAILGLDFADVAAVAKDAAQGDVCQ
                     AANDNSSGQVVLSGHKAAVDRACELAKARGAKRAILLPVSAPFHCALMQPAADAMAEA
                     LMNVTMNKPSVPLVANVVAGPISDPAEIRKRLVEQVTGTVRWRECVGFMAGQGVKLFC
                     EVGTGKVLTGLLKKNAPESQGMALGTPDEIAAAAAVLKQG"
     gene            complement(1337..2416)
                     /locus_tag="PKV23_06055"
     CDS             complement(1337..2416)
                     /locus_tag="PKV23_06055"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012562312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HQX85033.1"
                     /translation="MSRIEVRKGMPDVQLDRAEYERRFRNRFFDPAFAKNEAALKAII
                     DTAWEGYDVYRKNPIKRVAGVGYARPKAELPVEWLETKAAIDAAEARQKNPKSPSRIL
                     LVNGSMRSDQSCPGEMSKTWRLLKLAEEVVHAATGFETEILDLSLLTSQYGRVIHPCK
                     ACVSTAMPLCHWPCSCYPNFAMGQTSDWMNEIYPMWAAAHGVMILTPVNWYQAPAGLK
                     AMIDRLVCADGGNPDPTTTGGKDPQKAKEIELKGWDYPRHLAGRAFSVVVHGDAAGVE
                     NLRRILTDWLEDIGMIAAGHKSHIGAYIGYLGTYAESHIDLDRDEGVQADVKTAAQAL
                     VNAVTMLRAGKLNPPDAQLHEARPK"
BASE COUNT          509 a          803 c          825 g          547 t
ORIGIN      
        1 gcagggtcgc tgagattgca ggggagaaca tgaacgcgcc ctcccaaagc cgccttcagt
       61 tcttccagca cggcagcgcg ggttccggaa accgcaatgg tggcccctgc cccatgcagc
      121 gccttggcaa tgccggcgcc gataccgccg gtggcgccgg taatgagtgc ggtttttccc
      181 gtgagatcaa acatcgactt atccttgctt caagactgca gcggctgccg cgatttcatc
      241 aggggtgccg agggccatgc cctgggattc cggggcattc tttttcagga gtccggtcaa
      301 taccttgccg gtgcccactt cacaaaacag tttaacgccc tgccccgcca tgaagcccac
      361 gcattcgcgc cagcgcaccg tgccggttac ctgctccacc agccgcttgc ggatttcggc
      421 gggatcagaa atcggtccgg cgacgacatt cgcaaccaaa ggaactgacg gcttattcat
      481 ggtcacgttc atcaaggctt cggccatcgc atctgcagcg ggctgcatca gggcgcagtg
      541 aaagggggcg ctgacgggaa gcaaaatggc gcgctttgcg cccctggcct tggccagttc
      601 gcaggcgcgg tccacggccg ccttgtggcc ggagagcacc acctggccgc tgctgttgtc
      661 attggcggcc tggcagacat cgccctgggc agcatccttg gccacggctg ccacatcggc
      721 aaagtcgagc ccgagaatgg ccgccatggc gccgatgcca agcggggttg cggcctgcat
      781 ggccttgccg cggatgcgca ggaggcgtgc ggtatcgctc agcgaaaaag tccccgccgc
      841 ggccagggcc gaatattcgc ccagcgaatg gccgccgacg aatttcgcgg ttgaggcaac
      901 cgtgaccccg tgttcggcct ggagcaccgc aaggaccgcc atgctcacgg ccatgagggc
      961 gggctgggca ttggcggtga gcgtcaggtc tgcttccggc ccttcccaca tgagggtgga
     1021 tagcttctgc gaaagcgccg catccacttc gtcaaagaca gtgcgcgccg caggaaaggc
     1081 atcggccagc gccttgccca tgccaaccgc ctggcttccc tggccgggaa acgtaaatgc
     1141 aatgctcaag cgagactcct caaatcactt ccggcgccac gaaacatggg ggagcagcgg
     1201 agtcaagtct aagtaccctc atgctgaggt gcgaccccgg acttgatccg ggggagcctc
     1261 gaagcatcca tgtccgcggt gagcgccctt cgaggccatg ctttgcatgg ctcctcaggg
     1321 tgaggatttg gagtccttac ttcggccgtg cttcatgcaa ttgggcgtcg ggaggattca
     1381 atttgccggc gcggagcatg gttaccgcat tcaccagcgc ctgggccgcc gttttcacat
     1441 ccgcctgcac gccctcatcc cggtcgagat caatgtggct ttcggcatag gttccgagat
     1501 agccgatgta agcgccgatg tgggatttgt ggccagcggc aatcatgccg atgtcttcga
     1561 gccaatcggt gaggatgcgc ctgaggtttt caactcctgc cgcgtcacca tggaccacga
     1621 ccgagaaggc acggccagcg agatggcgcg gatagtccca gcccttcaac tcgatttcct
     1681 tcgccttctg cgggtccttg ccgcctgtcg tggtgggatc gggattgccg ccatcggcgc
     1741 agaccagccg gtcgatcatc gccttcaggc cagccggagc ctgataccag ttcaccgggg
     1801 tcaggatcat cacgccatgg gccgccgccc acatgggata gatttcgttc atccagtcgc
     1861 tggtctgacc catggcgaaa ttgggatagc acgagcacgg ccaatggcag agcggcatgg
     1921 cggttgaaac gcaggccttg cacgggtgaa tgacacggcc atattgcgag gtgagcagcg
     1981 agaggtcgag aatctcggtt tcaaatccgg ttgcggcatg gacaacttcc tcggcaagtt
     2041 tcagcagccg ccaggttttt gacatttcgc cggggcagct ctggtctgag cgcatggagc
     2101 cattgacgag aagaatccgg ctcggcgatt ttggattttt ctggcgggcc tcggcggcat
     2161 cgattgccgc cttggtttca agccattcca ccggcaactc cgccttgggc ctggcatagc
     2221 ctacacccgc cacacgcttg atggggttct tgcgatagac gtcatagcct tcccaggccg
     2281 tgtcaatgat ggccttgaga gccgcctcgt tcttggcaaa ggcgggatcg aagaaacggt
     2341 tgcgaaagcg tcgctcgtat tcggcacggt ccagctggac atcgggcatg cctttgcgga
     2401 cttcgatgcg gctcatgatg atcctcccgg ttgcaagggc ggactttgcg ccgggatacc
     2461 gcagattggc aaatcactga gacacacaag gcggtgagga tggggtgaaa gctgctgctt
     2521 cctcatgccg aggtgcgaat tgtccgatat ctgtttaaat attacttgac actatattcc
     2581 tagtatgtga tttgtgttcc cgtttcgttc tttcaacagg ataagcattt atggactggg
     2641 agcacgccat caaacgcaac agcgaggtac tcgcggggat cgtt
//
LOCUS       DAORVN010000258         2684 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_35948_length_2684_cov_2.550019, whole genome shotgun sequence.
ACCESSION   DAORVN010000258 DAORVN010000000
VERSION     DAORVN010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2684)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2684)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2684
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35948_length_2684_cov_2.550019"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1496
                     /locus_tag="PKV23_06060"
     CDS             <1..1496
                     /locus_tag="PKV23_06060"
                     /inference="COORDINATES: protein motif:HMM:NF012927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQX85034.1"
                     /translation="RATNMRNDEYGGSLENRARLLREVIEDTKEAVGDRCAVPVRIAM
                     NEFLGPNGLEPAEVEDAIGLMAELPDLWDLSLSSWEKDSQTSRFSEEGFQEPYIKGVK
                     KLTTKPVVGVGRYTSPDTMVRVIKQGIMDMIGAARPSIADPFLPKKIEEGRLDDIREC
                     IGCNICVTGDFTMSPIRCTQNPTMGEEWRRGWHPERIRPAGNVKNILVVGAGPAGLEA
                     ARALGTRGHKVTLADARSEAGGRVALECRLPGLAAWGRVRDWRLLQIAKLPDVQLYLE
                     SQMTPDTLAEFEADHVVLATGSSWRRDGVARHHTRPIPIAGNADVLTPDDILDGKRPK
                     GKDLVIYDDDHYYMGGVIAELLRKEDYAVTLVTPFYLVSSFTKASLEQHAIQSRLLGL
                     DVKVEANQGLASVSAGHVTLACVFTGRIRELEAGSVVLVTARNVRDHLFQTAAEKINN
                     LACIGDCFSPGTIAAAVHAGRKFAEEFGSPLPDFLDLPYRREVIGLS"
     gene            1644..>2684
                     /locus_tag="PKV23_06065"
     CDS             1644..>2684
                     /locus_tag="PKV23_06065"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HQX85035.1"
                     /translation="MAHAQTAEITGSITSIAPKLAVAGKLATKTEFLADRQEAAMVTA
                     GSIRALEGAIQLYEEFVAGGGWPSIGKGKLEKDAKSELVVTLRKRLVAEGYLPFEDMF
                     VEAANVFDSNLIEAVKFFQTSHGIASSGKVDDRIRAELNITARARLQTLRENYPRVGE
                     YSLDLHERNILVNIPSAQLETVEFGKVRSRHNVVVGKRDRPTPALKSVVSDINFNPYW
                     NAPASIVQKDLVPKFLKDPTIFEQMNIRIFDGVGGPEIDPYTVDWVNTHPERYHFRQE
                     PGESNALATMKINFANKHMVYMHDTPHREMFGSNARFESSGCVRVDQVSDVVNWILGG
                     QDGIDAAEIELIT"
BASE COUNT          544 a          832 c          764 g          544 t
ORIGIN      
        1 cccgcgccac caacatgcgg aacgatgaat atggtggttc cctcgaaaac cgcgcccgcc
       61 tgctccgcga ggtgattgag gacaccaagg aagcggtagg cgaccgctgc gccgtgcccg
      121 tccgcattgc catgaatgaa tttctggggc caaacggcct tgagcccgcc gaggtggaag
      181 atgccatcgg cctgatggcg gaactgcccg acctgtggga cctgtcgctt tcaagctggg
      241 agaaggattc acaaacctcg cgcttctccg aggagggctt tcaggagccc tatatcaagg
      301 gcgtgaaaaa gctgaccacc aagccggtcg tgggagttgg ccgctacacc tcgccggaca
      361 ccatggtgcg cgtgatcaaa cagggcatca tggacatgat cggcgccgcc cgcccttcca
      421 ttgcagatcc cttcctgccc aaaaagattg aggaaggccg cctcgatgat atccgtgaat
      481 gcattggctg caatatctgc gtgaccggtg atttcaccat gtcgcccatc cgctgcaccc
      541 agaatcccac catgggggag gaatggcggc gcggctggca ccccgaacgc atccggcctg
      601 ccggaaatgt gaagaacatc ctcgttgttg gcgccggtcc cgcaggactg gaagcggccc
      661 gggccctcgg aacgcgcggt cacaaggtga cgctggccga tgcccgaagc gaggccggcg
      721 gccgcgtggc gctggaatgc cgcctgcccg gccttgccgc ctggggccgg gtgcgcgact
      781 ggcgtttgct gcaaatagcc aagctgcccg atgtgcagct ctatctcgaa agccaaatga
      841 cccctgacac cctggcggag ttcgaagccg accatgttgt cctggctact ggttcaagct
      901 ggcggcgcga tggcgtggcc cgtcaccata caaggcccat cccgattgcc ggcaacgccg
      961 atgtcttgac gccggacgat atcctcgatg gcaagcggcc caagggcaaa gacctcgtca
     1021 tctatgacga tgaccattac tacatgggcg gcgtgattgc cgagttgctg cgcaaggaag
     1081 actatgcggt aactctggtt accccgtttt accttgtctc gtccttcacc aaggcatccc
     1141 tggagcagca cgcgatccaa tcccggttgc tcggactgga tgttaaagtt gaagcaaatc
     1201 agggcctcgc ttccgtttcc gccggccatg tcacgctggc ctgtgtcttt accggccgta
     1261 tccgtgagct cgaggcgggt tctgtcgttc tggtcaccgc cagaaacgtc agggaccatc
     1321 tcttccaaac ggctgcggaa aaaataaata atttggcctg cattggcgac tgttttagcc
     1381 ccggaaccat cgccgccgcc gtgcatgccg gccgcaaatt tgctgaggag ttcggctcgc
     1441 ccctgcccga cttcctcgac cttccctatc gcagagaagt gatcggtcta tcttaatcag
     1501 gccacaactt tttaatatgc cctttaccca gatcagtgat tctcggtttg tagcttctca
     1561 ggtctagggg tattggggtg ttttctaagc atttaacgcg tcgtgccttt ctcggtgctg
     1621 cctccctggc ggccgccgcc tccgtggcac atgcccaaac cgctgaaatt accggttcaa
     1681 tcacgtctat cgccccgaaa ctggccgtcg caggcaagct tgccacgaaa accgagtttc
     1741 tggctgaccg tcaggaagct gcgatggtca ccgcgggttc gatccgcgcc cttgaaggcg
     1801 ccatccagct ctacgaggag ttcgtggccg gtggcggctg gcccagcata ggcaagggca
     1861 agcttgaaaa ggacgcaaaa agcgaactgg tcgtgacttt gcgcaagcgt ctggtggcgg
     1921 agggctattt gcccttcgag gatatgttcg tggaagcggc caacgtcttt gactccaacc
     1981 tcattgaggc ggtcaaattc ttccagacca gtcacggcat cgcgtcctcc ggcaaggttg
     2041 atgaccgcat ccgcgccgag ttgaacatca ccgcccgcgc caggctgcaa accctgcggg
     2101 aaaactatcc ccgcgtcggc gagtattccc ttgacctgca cgagcgcaac attctggtga
     2161 acatcccctc cgcccagctt gaaaccgttg agttcggcaa ggtccgttca cgccacaatg
     2221 tggttgtcgg caaacgcgac cggcccacgc cggcccttaa aagcgttgtc agtgatatca
     2281 acttcaatcc ctactggaat gccccggcct ctatcgtgca gaaggacctg gtgcccaaat
     2341 tcctcaagga cccgacgatt tttgagcaga tgaacatccg gatttttgat ggcgtcggcg
     2401 gtcccgagat tgacccctac acggttgact gggtcaacac ccaccccgag cgctaccatt
     2461 tccgccagga gcccggcgag agcaacgccc tggccaccat gaagatcaat ttcgccaaca
     2521 aacacatggt ttacatgcac gacacgccgc accgcgaaat gttcggcagc aatgcccgtt
     2581 ttgaaagttc cggctgcgtc cgtgttgatc aggtcagcga tgtcgtgaac tggatcctcg
     2641 gtggtcagga tgggatcgac gccgccgaga tcgagctgat cacc
//
LOCUS       DAORVN010000259         2677 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_36106_length_2677_cov_3.842105, whole genome shotgun sequence.
ACCESSION   DAORVN010000259 DAORVN010000000
VERSION     DAORVN010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2677
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36106_length_2677_cov_3.842105"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..765
                     /locus_tag="PKV23_06070"
     CDS             <1..765
                     /locus_tag="PKV23_06070"
                     /inference="COORDINATES: protein motif:HMM:NF015436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HQX85036.1"
                     /translation="RVDDVMVPRADIIAIDDSATMRELLAKFNEANHSRLPVYRETLD
                     DVAGMIHVKDFMRWMSMRGAKSKTKAKNPGINISAKDLALPVKQSGIYREVLFVPPSM
                     PAADLLLKMQATHIHMGIVIDEYGGSDGLISIEDLVEEIVGDISDEHDTDDEQLIKRH
                     DDSTFVADARVQISVVDELLGVDLLSDEEEDEADTLGGLIFEMAGKVPTRGEIIKHPS
                     GLEFEILQSDARRVKRIRIHVKQKPGSDDAAEDTTG"
     gene            complement(762..1754)
                     /locus_tag="PKV23_06075"
     CDS             complement(762..1754)
                     /locus_tag="PKV23_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007008108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine kinase"
                     /protein_id="HQX85037.1"
                     /translation="MQAEIDVLTIGNAIVDVIAKVDDAFVQKNGLVKGSMNLIDEARA
                     EALYASMGPAIEISGGSAGNTAAGVASFGGKAAYFGKVKADQLGGIYAHDMRAQGVRF
                     ESKPATDGPATARSFILVTPEGERTMNTYLGACVHLTTADVDENTVSAAKVTYMEGYL
                     WDRPEAKEAFKLAAKIARKAGRLTSITLSDSFCVERHRESFLELIRKDIDIVFANENE
                     IKSLYLTQNFDGALQALRKDCEIAVVTRSALGSVVAHGDDVLVVPAIAVPKVVDVTGA
                     GDLYAAGFLFGYTQGFGNARSAQLGSLAAAEVISHVGARPEVNLRAHAKEAGLL"
     gene            1836..2498
                     /locus_tag="PKV23_06080"
     CDS             1836..2498
                     /locus_tag="PKV23_06080"
                     /inference="COORDINATES: protein motif:HMM:NF018917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EI24 domain-containing protein"
                     /protein_id="HQX85038.1"
                     /translation="MITAAFKALGDLMSRDFRAILFRAVGMTLGLFIAILIGVEVLLS
                     YLLSLPWPWAETLAGIGTFLVLLVAFFFMMAPVTAIFAGLYLDDVAGRVEAKHYRRDP
                     KGTPLSGFRALQTSLQFALVILLVNIAVLPLVFTGIGALALIIANAYLLSREFFEMIA
                     MRHMTVDEARQLRKINTPRILAAGFAPAILSVIPVVNLVVPLFATSYFTHIFKQVRAT
                     SP"
     gene            complement(2470..>2677)
                     /locus_tag="PKV23_06085"
     CDS             complement(2470..>2677)
                     /locus_tag="PKV23_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018425946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HQX85039.1"
                     /translation="NRTGLAPGKSPYDVEKGLLKAIPKKYLRHAHHWLILHGRYVCKA
                     RRPECWRCHIADICQFREKSPAPV"
BASE COUNT          519 a          826 c          782 g          550 t
ORIGIN      
        1 cgcgtcgacg acgtgatggt gccgcgggcc gacatcatcg ccatcgacga cagcgccacg
       61 atgcgcgagc tgctcgccaa attcaacgaa gccaaccatt cgcggcttcc ggtataccgc
      121 gagacccttg atgacgtcgc cggcatgatc cacgtgaagg acttcatgcg ctggatgtcg
      181 atgcgcgggg ccaaatcaaa aaccaaggca aagaatccgg gcatcaacat ctcggccaag
      241 gacctggcgc tgccggtcaa gcaatcgggc atctaccggg aagtgctctt cgtgccgcct
      301 tcgatgccgg ccgctgacct gctgctcaag atgcaggcca ctcatatcca catgggcatc
      361 gtcatcgacg aatatggcgg cagcgacggg cttatttcca tcgaagacct ggtcgaggaa
      421 atcgtcggcg acatctccga tgagcacgac accgacgacg agcaattgat caagcgccac
      481 gatgacagca ccttcgttgc cgatgcgcgg gtgcaaatct ccgtggttga cgagttgctc
      541 ggcgtagacc ttctctcgga cgaggaagag gacgaagccg acaccctcgg cggccttatt
      601 ttcgagatgg cgggcaaggt tccgacacgc ggcgaaatca tcaagcatcc aagcggcctg
      661 gaattcgaga tcctgcagtc ggacgcgcgg cgggtcaaac gcatccgcat tcacgtgaag
      721 cagaagcccg gctccgatga cgcggctgaa gatacgacgg gttagagcag gcccgcttct
      781 ttcgcgtgag ccctgagatt gacttcggga cgggcgccca catgggaaat gacttcggcg
      841 gcggcgagac tgcccaattg cgccgagcgg gcattgccga agccttgcgt gtaaccaaaa
      901 agaaagcccg cggcgtagag gtctcccgca cccgtcacat ccaccacctt tggaacagcg
      961 atggccggaa ccaccaggac atcgtcgccg tgggccacca cgctgcctag ggccgagcgt
     1021 gtcaccacgg cgatttcaca atccttgcgt agcgcctgca acgcgccgtc aaaattctgc
     1081 gtcaggtaga gcgacttgat ttcgttttca ttggcaaaga cgatgtcgat gtccttgcgg
     1141 atcagttcga gaaagctctc gcggtggcgc tcaacgcaga aggaatcgga gagcgtgatt
     1201 gaggtgagcc tgcctgcttt gcgcgcgatt ttcgccgcca gcttgaaggc ctccttggct
     1261 tccggcctgt cccagagata gccttccatg taggtgactt tggcggcgga aacagtgttc
     1321 tcatcaacgt cagcagtggt gagatgcacg caggcgccga gataggtatt catggtgcgc
     1381 tcgccctcgg gcgtcaccag gatgaaggag cgcgccgtgg ccggtccatc ggttgcgggc
     1441 ttcgattcaa agcgcacgcc ctgggcccgc atgtcgtggg catagatgcc gccgagctga
     1501 tcggctttca ccttgccgaa ataggccgcc ttgccgccga aggaagccac acccgccgcg
     1561 gtgtttccgg ccgagccgcc ggagatttcg atggccggcc ccatgctggc atagagcgcc
     1621 tcggcgcggg cctcatcgat gaggttcatc gagcccttga ccaggccgtt cttctgcacg
     1681 aaggcgtcat cgaccttggc aatcacatcg acaatcgcgt tgccaattgt cagaacatca
     1741 atttcagcct gcacgataca aaatctcccg aattcagcac aggccgtagc ggaagccact
     1801 tgcggaggca agcccgaagc cctacctacg cgcccatgat tacagccgct ttcaaagccc
     1861 tgggcgacct catgtcacgg gacttccgcg ccatcctgtt cagggccgtc ggcatgacac
     1921 tgggactctt tatcgccatc ctgattggcg tggaggttct tctatcctac cttttgtccc
     1981 tgccctggcc ctgggccgaa accctcgcag gcatcggcac atttctggtt ctgctcgtgg
     2041 ccttcttctt catgatggcg ccggtcaccg cgatcttcgc cggcctctat ctcgatgatg
     2101 tcgccggccg ggtggaagcc aagcattatc gccgggatcc gaagggcacg ccgctttcag
     2161 ggttccgcgc gttgcagact tcgttgcagt ttgccctcgt cattcttctg gtgaacatcg
     2221 cggtgctgcc gctggtcttc accggcatcg gcgcattggc cctcatcatt gccaatgctt
     2281 atctcctgtc gcgggaattt ttcgagatga ttgccatgcg gcacatgacc gtcgacgagg
     2341 cgcgccagct tcgcaagatc aacacgccgc ggattcttgc ggcgggattt gcgcctgcca
     2401 tcctgtcggt cattccagtt gtaaatctgg tggtgccgct gttcgccacc tcctacttca
     2461 cccacatttt caaacaggtg cgggcgactt ctccctgaac tggcatatgt cagcgatatg
     2521 gcagcgccag cattcgggcc tgcgcgcctt gcagacgtag cggccatgca ggatcagcca
     2581 gtgatgggca tggcgcagat attttttggg aatggccttc agcagccctt tttccacgtc
     2641 atagggagat ttccccgggg ccagcccggt gcggttg
//
LOCUS       DAORVN010000260         2672 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_36205_length_2672_cov_3.685900, whole genome shotgun sequence.
ACCESSION   DAORVN010000260 DAORVN010000000
VERSION     DAORVN010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2672
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36205_length_2672_cov_3.685900"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            250..375
                     /locus_tag="PKV23_06090"
     CDS             250..375
                     /locus_tag="PKV23_06090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85040.1"
                     /translation="MFTAILCAVALAAAYAAGVYTGLSKDKIIAFAKGLKAKVGL"
     gene            375..1004
                     /locus_tag="PKV23_06095"
     CDS             375..1004
                     /locus_tag="PKV23_06095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85041.1"
                     /translation="MEPASIRYKNPGAMWGSALAIKWGAAPKAVVLNDGKGQGNNIAV
                     FPTYVQGICAQLDLWRTSKNYKNKRFSDAIAIWSGGNNVESYIQFVLARVPGMTRNTV
                     MDDAFWRSSMGLAFLKAQAWHEAGKKYPAPDADWIEAQRLVFLGLAPATPPKPAPAKP
                     PVTKSEKAIVVATTATAGAATIQYGVWAAVTVVAIFAISAFVYFKFLRK"
     gene            1006..1596
                     /locus_tag="PKV23_06100"
     CDS             1006..1596
                     /locus_tag="PKV23_06100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85042.1"
                     /translation="MDWKSVSGSLIKAGAPIIGSALGGPLGGLIGSGLGDVLANALGV
                     EATPEAVNNAIVNGDPATVTAALSKAETEACARYSYLTAVVQSNAAQSDSINQTIRAE
                     LASGQRWFAWRNLWGYSCAVEASLTSLLVLYSLALNPQIVKNIIDSYSFFLSWYALRF
                     GVLGYIHNGASNEKIAAVTGEAPSIIKNIAKAVRGK"
     gene            1593..1823
                     /locus_tag="PKV23_06105"
     CDS             1593..1823
                     /locus_tag="PKV23_06105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85043.1"
                     /translation="MTAHEDTLRERVARLETKVDHLSEALDKAITKVDEMHGVMMQAK
                     GARWVLIAAASIAGALAGFAAKFTGLLSGPPR"
     gene            2104..>2672
                     /locus_tag="PKV23_06110"
     CDS             2104..>2672
                     /locus_tag="PKV23_06110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85044.1"
                     /translation="MAAATLTDDQLREAVALFETHNRNQQHAANAAGMSRSTFQSRLR
                     TAELRGITAQKPMLETESLIFPDLPSSELPAEQLIDQACARFAGHLAAREARRWMEIK
                     VKSNKPIGVCFMGDPHIDNNGCNWPLLRRDIKVLEETPGMYAVNIGDLTDNWVGRLVR
                     LYADQEMSKKQAWKLAKYLMRDSGIKWLC"
BASE COUNT          574 a          771 c          762 g          565 t
ORIGIN      
        1 ccaatactgg ctcgggcggc ggcggtgcgg gaacatcaaa tcaaacaggc ggcaacggcg
       61 gctccggtat cgtggttatt tcgtaccaat atcaataagg cataattcgc gacatgatca
      121 cgatgtacgg agtgaagtag ccgcaaacct aaacggcgag gcccatgctt cgccccgcgc
      181 tcaccatcct gatcctcctg cccatcatcg tgatcaggtt catcacctac taaacctctg
      241 gagcatcaca tgttcactgc aattctctgc gccgtggctc tcgctgcggc ttatgccgct
      301 ggcgtctata ccggcttgtc caaggacaag atcatcgctt ttgccaaggg cctgaaggca
      361 aaggttggcc tctaatggag ccagcatcga tccgctacaa aaaccccggc gcgatgtggg
      421 gaagtgctct cgccatcaag tggggcgctg cgcctaaggc cgttgtcctt aacgacggca
      481 aggggcaggg caataacata gccgtcttcc ccacctatgt gcagggaatc tgcgcccagc
      541 tcgacctgtg gcgcacatcc aagaactaca agaacaagcg attttctgac gccatcgcca
      601 tttggtccgg cggcaacaac gtcgagagct atatccagtt cgtactagcc cgcgtgccgg
      661 gcatgacccg caataccgtc atggacgatg ctttctggcg cagctccatg ggcctcgcat
      721 tcctgaaggc gcaggcatgg catgaggccg ggaagaagta tccggctccc gatgctgact
      781 ggatcgaggc gcagcggttg gtgttcctgg gtctggctcc tgccacgccg ccaaagccag
      841 cacccgcaaa accgcctgtc actaagtcag agaaagccat cgttgtggct acgaccgcca
      901 cggcgggagc ggcgacaatt caatacggcg tttgggctgc tgtcactgtc gtggcgatct
      961 ttgccatctc cgcgttcgtg tacttcaaat tcttgaggaa gtgagatgga ctggaaaagt
     1021 gtttcaggct cgctcatcaa agcgggcgcg ccgatcattg gctcggcctt gggtggtccg
     1081 cttggtggac ttatcggtag cggtcttggc gatgtactgg ctaatgcttt gggtgtggag
     1141 gctacgccgg aggcggtgaa caacgccatc gtcaacgggg atccagcgac ggttacggcg
     1201 gctctcagta aagcggaaac cgaggcttgc gcccggtaca gctatctcac ggctgttgtc
     1261 caatcgaacg cggcgcagtc tgacagcatc aaccagacaa tccgcgcaga actcgcttcg
     1321 gggcagcgct ggttcgcgtg gcgcaatctc tggggatatt cctgcgccgt ggaggcgtcc
     1381 cttacatcgc tcctggttct ctacagtctc gcgctaaatc cgcagatcgt gaaaaacatc
     1441 atcgactcct acagcttctt tttgtcgtgg tacgcgcttc ggttcggtgt tctcggctac
     1501 atccacaatg gcgcttcgaa tgagaaaatt gccgccgtaa ctggtgaagc tccgagcatc
     1561 atcaagaaca tcgccaaagc ggtgcgcggc aaatgacagc ccacgaagac accttgcgcg
     1621 aacgtgtcgc ccggcttgaa acgaaggtcg atcatctctc agaggcgctc gataaggcca
     1681 tcacaaaggt ggacgagatg cacggcgtca tgatgcaggc caagggtgcc cgctgggtgc
     1741 tgattgctgc ggcgtcgatt gcgggcgctc ttgctggttt cgcggccaag ttcaccggcc
     1801 tgcttagcgg cccgcctaga tgaaaatcat cgcgctcctg atcattctag gtctagcgca
     1861 atggtatttc cgaaagcctc cacctccagt cgttcatatc gtcccgcatt gcctggacac
     1921 caatcgcggc tggtttctgc cttgtactga cgttgatcga tacgagtaca cttaaccaca
     1981 caccaactta tggggtttca tgttctcaaa tccggcagcg gaatttgcga cgtccatcat
     2041 cattgcattg gggtttctag ggatcgctga gacgatctgg ttgttgcggc gggggtggct
     2101 gtaatggcgg cagcaacact cactgatgac cagcttagag aagccgtcgc cctttttgag
     2161 acgcacaatc gaaatcagca acacgccgcc aatgctgccg gaatgtcccg cagcaccttc
     2221 caatccaggc ttaggacggc ggaactgcgt ggtatcacgg cacagaagcc gatgctggaa
     2281 acagaaagcc tgatcttccc cgatttgccg tccagcgaac tacccgctga gcagttaatc
     2341 gatcaggcgt gcgccagatt cgccggccac ctagccgccc gtgaggcgcg gcgatggatg
     2401 gagatcaagg tcaagtccaa caagcccatt ggcgtgtgct ttatgggcga cccgcacatt
     2461 gataacaacg gctgcaactg gccgctcctt cgccgggaca tcaaggttct ggaagagacg
     2521 ccgggcatgt acgccgtgaa cattggcgac ttaacggaca attgggtcgg gcgcctcgtt
     2581 cgcctctatg ccgatcagga gatgtcaaag aagcaggcgt ggaagctcgc aaaatacctc
     2641 atgcgggatt ccggcatcaa atggctgtgt ca
//
LOCUS       DAORVN010000261         2662 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_36436_length_2662_cov_3.040276, whole genome shotgun sequence.
ACCESSION   DAORVN010000261 DAORVN010000000
VERSION     DAORVN010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2662
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36436_length_2662_cov_3.040276"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1335
                     /locus_tag="PKV23_06115"
     CDS             <1..1335
                     /locus_tag="PKV23_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015343352.1"
                     /note="has catalase and peroxidase activities; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase-peroxidase"
                     /protein_id="HQX85045.1"
                     /translation="IEEQGLGWKSSFGSGKGGDTISSGLEVTWTTTPTKWSNNFFWNL
                     FGYDWELMKSPAGAHQWKPKHNAGAGSVPDAHDKTKRHAPTMLTSDLALRFDPIYEKI
                     SRRFHENPDQFADAFARAWYKLTHRDMGPLVRYLGPLVPKEELPWQDPIPAVNHPLIG
                     DADIAALKGKILASGLTVSQLVTTAWAAASSFRGTDKRGGANGGRIRLAPQKDWDVNQ
                     PAQLAKVLQKLEAIQKEFQSGGKKVSLADLIVLGGAAAIEKAAKDGGHDIKVPFTPGR
                     MDASQEQTDVESFEPLEPTADGFRNYLRGKQRLSPEEQLVDRAQLLSLTAPEMTVLVG
                     GLRVLNAGQPAHGVFTKKPETLTNDFFVNLLDMSTVWKAISDSKEVFEGRDRKTNEVK
                     WTGTRVDLIFGSHSQLRALAEVYAQADSKAKFAKDFVAAWNKVMNADRFDLA"
     gene            1641..>2662
                     /locus_tag="PKV23_06120"
     CDS             1641..>2662
                     /locus_tag="PKV23_06120"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015929909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HQX85046.1"
                     /translation="MNFSELGLSPKVLEAISAAGYTTPTTIQAEAIPHALQRKDVLGL
                     AQTGSGKTAAFVLPMLTLLEKGRARARMPRTLILEPTRELAAQVHEAFETLGKQHKLT
                     VALLIGGVAFEPQAAKINRGADVVIATPGRLLDHMERGGLLLTGIEMLVIDEADRMLD
                     MGFIPDIEKICKLLPFTRQTMFFSATMPPEIHRLTTQFLHNPVRIEASAPSSTAASIE
                     QYLVKVPHDAAPKRDALRALLRTQTKVKNGIVFANRKTTVALLEKSLRKHGFSAGALH
                     GDMDQSARLKMLAAFRNNEVTYLIASDVAARGLDIPEVSHVFNFDMPIHAEDYVHRVG
                     RTGRAGR"
BASE COUNT          584 a          803 c          780 g          495 t
ORIGIN      
        1 attgaagagc aaggcctcgg ctggaagagc agctttggca gcggcaaggg cggcgacacg
       61 atcagcagcg gcctggaagt cacctggacc acgacgccaa ccaagtggag caacaacttc
      121 ttctggaacc tgttcggcta tgattgggaa ctgatgaaga gccccgccgg ggcgcaccag
      181 tggaaaccga agcacaatgc gggtgcgggt tcagtgccgg atgcgcacga caagactaag
      241 cgccatgcgc caaccatgct gacctctgat ctcgctcttc gcttcgatcc gatctacgaa
      301 aaaatttccc ggcgcttcca cgagaatccg gaccagttcg ccgatgcgtt cgccagagcc
      361 tggtacaagc ttacccaccg cgacatgggc ccgctcgtgc gctatctcgg cccgctggtt
      421 ccgaaggagg aactgccgtg gcaagaccct atccccgctg taaatcatcc gctgatcggg
      481 gacgccgaca ttgctgcgct caagggcaag atccttgcgt ccggtcttac cgtgtcgcaa
      541 ctggtcacaa ccgcctgggc cgccgcctcg tcattccgcg gcactgacaa gcgcggtggc
      601 gccaatggcg ggcgcattcg actcgcgccc caaaaggatt gggacgttaa ccagccggcg
      661 caactggcca aagtgcttca gaaacttgag gcaatccaaa aggagttcca gtccggcggc
      721 aagaaagtct cgctggcaga cctgatcgtt cttggcggtg ctgcagcaat tgagaaagcc
      781 gccaaagacg gcggacacga catcaaggtg ccgttcacgc cggggcgcat ggatgcgtcg
      841 caagagcaaa ccgacgtgga gtcctttgag ccgctagaac caaccgctga cggtttccgc
      901 aactatctgc ggggcaagca gcggctgtcg ccggaggagc agttggtgga ccgggcgcaa
      961 ttgctgtcgc tgacggcacc tgaaatgacg gtgctggtag gcggcctgcg ggtgctgaat
     1021 gccggacagc cggcgcatgg cgtgttcacc aaaaagcccg agactctcac gaatgacttt
     1081 ttcgtcaacc tgcttgacat gagcacggtc tggaaagcga tttcggactc caaggaggtg
     1141 ttcgaaggcc gtgaccggaa aacgaacgaa gtcaagtgga ccggtacccg ggttgatctg
     1201 atcttcggtt cacattcgca gctccgcgct ctggcggaag tctatgcgca ggcggactcg
     1261 aaagcgaagt tcgcgaagga ctttgtggca gcgtggaata aagtgatgaa tgcggaccgc
     1321 tttgacctgg cctgacctct tcggagagcc ccggcggggg cgtttgccca ccgcctcaat
     1381 caaaggtggc atcctcagct tcgagggagt aaggttaagg agaacgattc gggaaatgac
     1441 ggtaacctcc caagcgtaac aaactcaaag ggcggctctt agatccgccc ttttttaccg
     1501 tttaaccctt tgacttaagt catttgactt taattggaaa agccctattt tccgcctcaa
     1561 tcagcgcgcc cccggatgtg gcgaaacggc cccgggcgct caccctttat caaatcgcca
     1621 gaacggtctt tcaaccctag atgaatttct ctgaactcgg actaagtcca aaagtactcg
     1681 aagctatttc agctgccgga tataccaccc ctacgaccat ccaagcggaa gcaattcccc
     1741 atgccttgca gcgcaaggac gtgctgggac tggcgcagac cggctcgggc aaaacggcgg
     1801 cctttgtgct gccgatgttg accctgctgg aaaaaggccg ggcccgggcc cgcatgccac
     1861 gcaccctcat tctggaaccc acccgcgaac tggcggcgca ggtccatgaa gccttcgaga
     1921 cgctaggcaa gcagcacaag ctgaccgttg cccttctcat cggcggcgtt gcctttgagc
     1981 cacaggcagc caagatcaac cgcggcgccg atgtggtgat cgccacgccg ggacgcctgc
     2041 tcgaccacat ggagcgtggc gggttgctgc ttaccggcat cgagatgctg gtgatcgacg
     2101 aagccgaccg gatgctcgac atgggcttca ttcccgacat cgagaagatc tgcaagctat
     2161 tgcccttcac ccggcagaca atgttcttct cggccaccat gccgccggaa atccaccggc
     2221 tcacaacgca gttcctgcac aatccggtac gcatcgaagc gagcgctccg agttcgacgg
     2281 ccgcatccat agaacaatat ctggtgaagg tgcctcatga tgcagcgccc aagcgcgatg
     2341 cattgcgtgc actgctgcgg acgcagacca aagtgaagaa cggcattgtc ttcgccaacc
     2401 gcaagacgac cgtggcgctt ttggaaaaat ccctgcgcaa gcacggcttc agcgcgggcg
     2461 ctttgcatgg cgacatggac cagtcggcac ggctcaagat gctggccgcc ttccgcaaca
     2521 atgaagtgac ctatctcatc gcctccgacg tggcggcgcg cggccttgat attccggagg
     2581 tgagccacgt gttcaatttt gacatgccta tccacgccga agactatgtg caccgggttg
     2641 gccgcacggg ccgtgcgggg cg
//
LOCUS       DAORVN010000262         2661 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_36467_length_2661_cov_2.900998, whole genome shotgun sequence.
ACCESSION   DAORVN010000262 DAORVN010000000
VERSION     DAORVN010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2661
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36467_length_2661_cov_2.900998"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..858
                     /locus_tag="PKV23_06125"
     CDS             <1..858
                     /locus_tag="PKV23_06125"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HQX85047.1"
                     /translation="RLFTEDMETGMRARRALEQLIGRATAQNGFELHYQPLLHAESGA
                     CAGFEALLRLPDGKGGYVSPLTCIPVIEAMGLINAVGKWVIEEATRTAATWPSHLFVS
                     VNLSVKQFEDGTLVGHVKRALDSSGLRPKQLELEVTETLLMQNTESTARQLAELRELG
                     VALSMDDFGTGYSSLGYLWQFRFDKLKIDRSFISALCHNEKKAVEILDTIIMLGHKLD
                     MTVTAEGIETAHQAEILVSLSCDHVQGYLYGPPAQASEIALFLLENTRTRLAPKKPVA
                     KIEARPAVA"
     gene            complement(881..>1399)
                     /locus_tag="PKV23_06130"
     CDS             complement(881..>1399)
                     /locus_tag="PKV23_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018496598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HQX85048.1"
                     /translation="EAGVLKPKNPGVTLNTAYMRPRSRGTVRLASNDPVAPPLIDPNY
                     WSDPYDKEMSLRGLRLARQIMKQPALKPFVLREIIPGSDLASDEELIAYGCVNAKTDH
                     HPVGTCKMGIDEAAVVTPDLKVRGIEGLRVCDASIMPRVTTGNTNAPTIMIAEKAADL
                     IRGITSAAPSTK"
     assembly_gap    1400..1409
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1410..2473)
                     /locus_tag="PKV23_06135"
     CDS             complement(<1410..2473)
                     /locus_tag="PKV23_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006163684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HQX85049.1"
                     /translation="MDYDYVIVGGGSAGCTLAARLSEDPQLQVLLLEAGGSDWNPLFH
                     WPAGFARMTKGIASWGWSTVPQKHMNGRVFRFTLAKVIGGGSTINAQIYIRGNKLDYN
                     EWAQLGCTGWAYDDVLPYFKRSEKNQNGASEFHGTSGPLGVSNPVAPLPICEAFFKAA
                     GELGIPHNRDFNGSQQDGVGHYQLTQSNARRSSVVSSFLHEARSRKNLTIRKNVFVKR
                     IVVEGGRATAVELAGETINAAREIILSSGTVGSPKLLMHSGIGPADQLKSVGIKPVHD
                     LSGVGENLQDHLDLFVICECTGDHTYDKYAKLHWSALAAIRYLALRNGPVASSLFETG
                     GFWYADENARSADIQFHLGLG"
BASE COUNT          544 a          715 c          837 g          555 t
ORIGIN      
        1 cgcctgttta ccgaggatat ggaaacaggc atgcgggccc ggcgcgcgct ggaacaactg
       61 atcggcaggg caacggcgca gaacggcttt gaacttcatt atcagccgct gctgcacgcg
      121 gaaagcggag cctgtgcggg ctttgaagcc ctgctgcgcc ttcccgatgg aaagggcggc
      181 tatgtttcgc cgctgacctg catcccggtg atcgaggcga tggggctgat caacgccgtt
      241 ggaaaatggg tcatcgagga ggcaacgcgg accgccgcca cctggccgtc acatcttttt
      301 gtttcggtca atctttcggt gaagcagttt gaggacggca cgcttgtcgg acatgtgaag
      361 cgggcactgg actcttccgg gttgcgccca aagcagcttg aactcgaagt caccgaaaca
      421 ctcttgatgc agaacacgga gtctaccgcc aggcagctcg ccgagttgcg cgaactcggc
      481 gtggcgcttt ccatggatga tttcggcacc ggttattcga gcctcggcta tctctggcaa
      541 ttccgtttcg acaagctgaa aattgatcgg tccttcatct cggccctttg ccataacgag
      601 aagaaggccg tggagattct cgatacaatc atcatgctcg gccacaagct cgacatgacg
      661 gtgacggccg aaggcattga gacggcccac caggcggaaa tcctagtgtc cctgtcctgt
      721 gaccatgtgc agggctacct ttacggcccc ccggcacagg ccagcgaaat tgcattgttc
      781 ctgctggaga atacgcggac ccgccttgcc cccaagaagc ctgtagcgaa aattgaggca
      841 agaccagccg tggcctgaaa cttcccagta gtcagctcaa tcacttcgtg gacggagcgg
      901 cagatgttat cccccggatc aggtcagcgg ccttttcggc gatcatgatg gttggggcat
      961 tggtgtttcc ggttgtaacg cgcggcatga tggaagcgtc acagacacga agtccctcga
     1021 tgccccgtac tttaaggtcg ggcgttacca cggcggcctc atcaataccc atcttgcagg
     1081 tgccaacggg atgatgatcg gttttggcgt tgacgcagcc ataggcgatc aactcttcat
     1141 ctgatgcgag gtccgaaccc gggatgattt cccgcaaaac aaagggcttg agcgccggtt
     1201 gcttcatgat ctggcgggca aggcgcaggc cgcgcagcga catttccttg tcgtaagggt
     1261 cggaccagta gttggggtca ataagtggcg gcgctaccgg gtcattgctg gcaaggcgca
     1321 cggtgccacg cgagcggggc cgcatatagg cggtgttcag ggtgacgccc ggatttttag
     1381 gtttgaggac gcccgcctcn nnnnnnnnnc ccagtcccag gtggaactgg atgtcagcgg
     1441 agcgggcatt ctcatcggcg taccagaagc cgccggtttc aaagagggat gaggcaacgg
     1501 ggccattgcg caaggcaaga tagcggattg ccgccagggc cgaccaatgg agtttggcgt
     1561 atttgtcata ggtgtggtcg ccggtgcatt cgcagatgac gaagagatcg aggtggtcct
     1621 gcaggttttc gccgacaccc gaaaggtcgt gaactggctt gatgccgacg gatttcaatt
     1681 gatctgccgg gccgatacct gaatgcatca gcagtttggg tgagccgacg gtgccggagg
     1741 atagtatgat ttcacgggcg gcattaatgg tttcgccggc aagctccacg gcagttgccc
     1801 tgccgccttc cacgacaatg cgcttgacga agacattctt gcgaatggtg agattcttgc
     1861 ggctccgggc ttcatgaagg aaactggaga cgacggagga ccggcgggcg ttcgactggg
     1921 tgagctgata gtggccgacg ccatcctgct ggctgccgtt gaaatccctg ttgtggggaa
     1981 ttccaagctc tcccgccgcc ttgaagaagg cctcgcaaat gggcagtggg gccacgggat
     2041 ttgaaacgcc cagggggccg gaagtaccat gaaactcaga ggcgccgttc tggtttttct
     2101 cggagcgctt gaaataggga agcacatcgt cgtaagccca gccagtgcag ccgagttggg
     2161 cccattcatt gtaatccagc ttgttgccgc ggatatagat ctgtgcgttg atggtggatc
     2221 cgccgccaat gactttggcc agggtgaagc gaaagacgcg gccgttcatg tgtttctggg
     2281 gcacggttga ccagccccag ctggcaatac ccttggtcat cctggcaaaa cccgctggcc
     2341 aatggaacag gggattccaa tcacttccgc ctgcctccag aagcaacacc tgaagctggg
     2401 gatcttcgct gaggcgggct gccagggtgc agcctgccga gccgccgccc acgatcacat
     2461 agtcatagtc catgatgccc ttcgcggatt gaagtgggtt tcctgaagcg gttagagtaa
     2521 cagatgcggg gacggctgca atcgtaacta ttttgttttg aatggcattc tgggatgggc
     2581 gacgtggtga cgtgggatga ggtgaggaac tccgggatcg gcaacttccc ggcactggtg
     2641 cgcgccagga tgcaggcacc a
//
LOCUS       DAORVN010000263         2660 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_36489_length_2660_cov_3.361996, whole genome shotgun sequence.
ACCESSION   DAORVN010000263 DAORVN010000000
VERSION     DAORVN010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2660
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36489_length_2660_cov_3.361996"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /locus_tag="PKV23_06140"
     CDS             <1..333
                     /locus_tag="PKV23_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019862553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HQX85050.1"
                     /translation="EINCMVGFYSYNPPKIYEALQAAGKLGAITVIAFDEDPITLGAV
                     KDGSFAGTVVQQPFEWGYRGMKLMAAYMEGDKSGIPENKLIIVPTKIITKDNVDQFEA
                     DLKAAIGK"
     gene            457..1941
                     /locus_tag="PKV23_06145"
     CDS             457..1941
                     /locus_tag="PKV23_06145"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009031150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HQX85051.1"
                     /translation="MIDIAKSYAGVQAVQGVNFSVYPGEVIGLVGENGAGKSTLMKII
                     GGVIEPSSGSLEIDGVQRKSLTVRESMGAGIAFVHQELNLFDNLDVASNVYIGREPLY
                     GGPFRLVDRKKLRADVQPLLERLGVDFTPETMVSDMSIAQRQLLEIAKALSLNSRLVI
                     MDEPTSSLTLIETDRLLNVVAGLKASGVSVIFISHRLNELTKCADRVVVLRDGRVVGE
                     LGKGEISHAAMIRLMIGRDLKALYVPPAARPGEAVLSVSDARTTAYPTQTVSLSVRQG
                     EILGLAGLVGAGRTELARALFGIDTLIAGSLILNGKTVEVGSPRQAIAQGIFLVPEDR
                     KRCGLLLDISIAENIALPNMKACASTGLVNEEVVAANAERQKKRLHIRAASVNQEGNS
                     LSGGNQQKVVLAKWLAMNPRVLICDEPTRGVDVGAKSELYEMLRGLADAGVAILMISS
                     DMEEVIGVSDRIAVMHEGMISGFLERAEFSEHNVLRLAVGESIH"
     gene            1955..>2660
                     /locus_tag="PKV23_06150"
     CDS             1955..>2660
                     /locus_tag="PKV23_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018445321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQX85052.1"
                     /translation="MFKKDIGLLLLILVVGTVVAVINPLFLSAINLGNTANLVGLFGI
                     FAIGQAFVIITGGIELSVGSIIALLGVLFVDLIVGHEMAWPLALVLMLALGVLIGLIH
                     GLLITRMHMQPFIVTLCGLLIYRGAARFYTQDGTAGFGFGVSFPQLEWLATGRSLGVP
                     HSFIALIIIACLMWVVLHRSVFGRYLYAVGKNEEAARYSGIRTNRVIIAAYVICDVLA
                     ALSAIFFAMYTRSILPS"
BASE COUNT          540 a          699 c          821 g          600 t
ORIGIN      
        1 gaaatcaact gcatggtcgg tttctactcc tacaacccgc caaagatcta tgaagccctg
       61 caggcggccg gaaagcttgg cgcgatcacc gtaatcgcct ttgatgagga tccgatcacc
      121 ttaggtgcgg tcaaggatgg cagctttgcc ggcaccgtcg tgcagcagcc ctttgaatgg
      181 ggctatcgtg gcatgaagct gatggccgcc tacatggagg gcgacaaatc cggcataccg
      241 gagaacaagc tgatcatcgt gcccacgaaa atcatcacca aggacaatgt cgatcagttt
      301 gaagctgatc tcaaagcggc aatcggcaag taagacaagt cgagccagcg cgggaagctg
      361 cgctggcttt cgcttgcgtc accggggaat gcctgtacag aatgcagctt gtctgctggg
      421 ggttcagttc tgaagatggc cggtgccttt tttcgaatga tcgatatcgc caagtcctac
      481 gccggcgtgc aagcggtgca gggggtgaat ttttccgttt atcccggcga agtgatcggg
      541 ctggtgggcg aaaatggggc tggcaaatct acgctgatga aaatcatcgg cggagtgatt
      601 gagccttcct cgggcagtct tgaaattgac ggcgtccagc gcaagtcact cacggtgcgc
      661 gagtccatgg gggcaggaat tgctttcgtg caccaggaac tcaacttgtt tgacaatctt
      721 gatgtggcgt cgaacgtgta tattggccgc gaaccgcttt atggcgggcc gttcaggctg
      781 gtggatcgca agaagctgag ggcggatgtg cagccgctgc tggagcggct gggcgtggac
      841 tttacacccg agaccatggt ttccgacatg tcgattgccc agcgccagct cctcgagatt
      901 gcgaaagcgc tctcgctgaa ttcacgtctg gtgatcatgg atgaaccaac ttcgagtctg
      961 acgctgattg agacagaccg ccttttgaat gtcgtggcgg ggctcaaagc ctcgggtgtc
     1021 agtgtcattt tcatttcgca ccgcctcaac gagttgacaa aatgcgcgga ccgggtcgtt
     1081 gttctgcgtg atgggcgcgt ggtgggcgaa ctcggcaaag gcgaaatcag ccacgccgcc
     1141 atgattcgcc tgatgatcgg gcgtgacctg aaggcgcttt atgtgccgcc ggcagccagg
     1201 ccgggagaag ccgtgctgag cgtttctgac gcgcgcacga ccgcctatcc cacccagacg
     1261 gtaagcctca gcgtgaggca aggcgaaata ctcggactag ccggtttggt tggggcaggg
     1321 cgaaccgaat tggcgcgcgc cctgtttggg attgacaccc tgattgcagg ttccctaatc
     1381 ctcaatggca agacagtcga agttggcagc ccgcggcaag ccattgcgca aggtatcttt
     1441 cttgtgcccg aggaccgtaa gcgctgcggg ctgctccttg atatatccat tgcagaaaac
     1501 atcgcgcttc caaacatgaa ggcgtgtgcc tcaacgggtc tggtgaacga ggaagttgtt
     1561 gcggccaatg cagagcggca aaagaaacgc ctgcatatac gcgctgccag cgttaaccag
     1621 gaaggcaatt cgctttccgg cggcaaccag cagaaagtcg tactggccaa atggctcgca
     1681 atgaaccccc gcgtcttgat ttgtgatgag cccacgcgcg gcgttgatgt gggagcaaaa
     1741 agcgaactct acgaaatgct gcggggactg gctgatgcgg gagtggccat actgatgatt
     1801 tcaagcgaca tggaagaagt gatcggggtg agcgaccgga tcgccgtcat gcacgagggc
     1861 atgatcagcg gttttctgga gcgggcggaa ttcagcgagc ataacgtgtt gcgtcttgcg
     1921 gttggcgaaa gcattcattg aagggacgag caacgtgttc aagaaagaca taggccttct
     1981 cctcctgatc ctcgtggtag ggactgtggt ggcggtgatc aatccgcttt tcctatcggc
     2041 gatcaatctg ggcaatacgg ccaatcttgt gggattgttc ggcatatttg ccatagggca
     2101 agcgttcgtc atcatcactg gaggtatcga gctttctgtg ggttcaatca ttgcgctgct
     2161 tggcgtgctg tttgttgacc tgattgtcgg ccacgaaatg gcctggccgc tggcgcttgt
     2221 gctcatgctg gcactgggcg tgttgatcgg actgattcat ggcctgctaa ttacccggat
     2281 gcacatgcaa ccctttattg tcactctctg cggcctgctg atataccgcg gagcggcccg
     2341 gttctacacg caggacggaa cagcgggctt cgggtttggg gtaagctttc cgcagttgga
     2401 atggctggcg acgggccggt cccttggcgt gccacattca ttcatcgccc tgatcattat
     2461 agcctgcctg atgtgggtgg ttttgcaccg ctcggtcttt gggcgctatc tctatgcggt
     2521 gggcaagaac gaggaggcgg cgcgctattc cggcatccgc accaaccggg tcatcatcgc
     2581 cgcctacgtc atctgcgacg tgctcgcggc gctctcggcg atcttcttcg ccatgtatac
     2641 ccgctcgatc ctcccgtccg
//
LOCUS       DAORVN010000264         2658 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_36549_length_2658_cov_2.875912, whole genome shotgun sequence.
ACCESSION   DAORVN010000264 DAORVN010000000
VERSION     DAORVN010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2658
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36549_length_2658_cov_2.875912"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..836
                     /locus_tag="PKV23_06155"
     CDS             <1..836
                     /locus_tag="PKV23_06155"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HQX85053.1"
                     /translation="NFNSVEEIADAKAEIFTGVTAGGHAVLNRDNHFFERLSAAARSA
                     GIRNIISFGSHEKADVRSERIVLHATCSCVTADVMGEKLIYKLGLPGEHMVLNSLAVL
                     AAVKVMGADLARAALALAVAEAAKGRGVHHRLRMEAGELLLIDESYNANPVSMAAALA
                     LLGRAKTGKGGRRIAVLGDMLELGKDGASLHAELFKAVDANKVDVVYAAGPLMAHLWA
                     KTPEDRRGASANTSAELKDRLLDGLQAGDVVMIKGSLGSRMGPLVDAIKERFAPLNRD
                     H"
     gene            840..1928
                     /gene="mraY"
                     /locus_tag="PKV23_06160"
     CDS             840..1928
                     /gene="mraY"
                     /locus_tag="PKV23_06160"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011503788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HQX85054.1"
                     /translation="MLYYLLTGLTDEFSVLNVFRYLTTRTGGAVLTALFFIFVFGPRI
                     IALLKVKQGRGQPIRSDGPQRHIIEKQGTPTMGGLMILSGTFVSTILWANLSNLYVWA
                     VIFVMLGFGAIGFYDDFLKVTRSTTKGFSGKARLLVEFLIAGVAVACFMWIGDKPLSS
                     SLAIPFFKDVLIPFGSFFIIVGVFVIVGAGNAVNITDGLDGLAIVPVMIAAASYGLIV
                     YLVGNAVFAEYLQVHFVKGTGELAVICGALVGAGLGFLWFNAPPAMIFMGDTGSLALG
                     GALGAIAVAVKHEIVLAIIGVLFVLETVSVIVQVASFKMTGKRVFAMAPLHHHFEQKG
                     WKEPTIVIRFWIIAFVLALIGLSTLKLR"
     gene            1938..>2658
                     /locus_tag="PKV23_06165"
     CDS             1938..>2658
                     /locus_tag="PKV23_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012456864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HQX85055.1"
                     /translation="MFPAHTFKNRKVGLFGLARSGLACAEALRLGGAEIFAWDDSEAA
                     VDAAKNANLLVSNLHDVDFAGLDSLVLSPGVPLTHPEPHWTVVKAKAAGIEIIGDTEV
                     FQREVAGTGARIVAITGTNGKSTTTALIGHIFQSLGRDVDVGGNIGTAVFLMRPPVEG
                     RIYVLELSSFQIDLMPGLRPEVGILTNLTPDHLDRHGSMDNYAAIKSRMFLKQDGNDT
                     AVIGVDDEWCAGIAIGIANGQK"
BASE COUNT          491 a          728 c          860 g          579 t
ORIGIN      
        1 gcaatttcaa ttcggttgaa gaaatcgccg atgccaaggc ggaaatcttc accggcgtaa
       61 cggctggcgg ccatgcggtg cttaaccgtg acaatcactt ttttgaacgg ctctcagccg
      121 cagcgcgcag cgccggcatc cggaatatca tcagttttgg ctcccacgaa aaggctgacg
      181 tgcgctcaga gcgcattgtg ctgcatgcga cctgcagttg cgtgacggcg gacgtgatgg
      241 gcgaaaagct catctacaag cttggcctgc ccggtgagca tatggtgttg aacagccttg
      301 cggtgctggc cgcggtgaaa gtgatgggag ccgacctggc ccgcgccgcc ctggcgctgg
      361 cggtggccga ggcggcaaag ggcaggggtg tacatcaccg cctgcggatg gaagccggcg
      421 aattgctgct gattgacgag agctataacg ccaatccggt ttccatggct gccgcgctgg
      481 cgcttctggg gcgggccaaa accggcaagg gcgggcggcg cattgcggtt ctgggcgaca
      541 tgctggaact cggaaaggac ggggcaagcc tccatgccga gcttttcaag gcagtggacg
      601 ccaacaaggt ggatgtcgta tatgccgccg ggcccctgat ggcccatctc tgggcgaaga
      661 cgccggagga ccggcggggc gcttctgcca atacgtccgc ggaattgaag gaccgtctgc
      721 tcgacgggct tcaagccggg gatgtcgtca tgatcaaggg ctcgcttggc agtcggatgg
      781 gaccgctggt ggacgccata aaagagcgct ttgcgccact gaacagggat cactagctga
      841 tgctttatta ccttctcacc ggcctcaccg atgaattctc ggtcttgaac gtgttccgtt
      901 acctcacgac acggaccggc ggggcggtcc tgacagcgct gttctttatt tttgtgttcg
      961 ggccaaggat cattgcgctg ctcaaagtga agcaggggcg gggacagccg atccgcagcg
     1021 acggcccgca gcgccatatc atcgaaaagc agggcacgcc gacgatgggc ggcctcatga
     1081 tcctctcggg cactttcgta tccaccatcc tgtgggccaa tctcagcaat ctctatgtct
     1141 gggcggtgat atttgtcatg cttggttttg gcgccatcgg cttctatgac gatttcctga
     1201 aagtgacgcg ttcgaccaca aagggctttt ccggcaaagc acggctgctg gtcgaattcc
     1261 tgatcgctgg cgtggcggtt gcctgcttca tgtggattgg cgacaagccg ctgtcgagtt
     1321 cgctcgccat acccttcttc aaagatgtgc ttatcccttt cggcagcttc ttcatcatcg
     1381 tgggggtgtt cgtcattgtg ggggccggca atgcggtaaa catcactgac gggcttgatg
     1441 ggctggccat tgtgccagtc atgatcgcgg cggcgagcta cggattgatc gtgtatctgg
     1501 tgggcaatgc ggtgttcgcg gaatacctgc aggtgcactt tgtaaagggc acgggcgaac
     1561 tagcggtgat ctgcggagcg ctggtggggg ctggccttgg cttcctgtgg ttcaatgcgc
     1621 ctcctgccat gatctttatg ggcgataccg gctcgctggc gcttggcggg gcgctcggcg
     1681 ccattgccgt tgcggtgaag catgaaatcg tgctggccat tatcggcgtc ctcttcgtgc
     1741 tggaaaccgt ttccgtgatc gtccaggtgg cctcctttaa aatgaccggc aaacgggtgt
     1801 ttgccatggc gccgctgcac caccacttcg agcagaaggg ttggaaggaa ccgaccattg
     1861 tcatccgctt ctggatcatt gcctttgtgc tggccctcat cggcctgtcg accttgaagc
     1921 tcaggtaggc acctcaggtg tttccggctc atacattcaa gaacaggaag gtggggctct
     1981 tcgggcttgc gcgctcgggg ctggcctgtg ccgaggccct gcggctaggc ggcgccgaga
     2041 tattcgcctg ggatgattcg gaagcagcgg tggacgcggc aaagaacgca aatctccttg
     2101 tttcgaattt gcatgacgtg gacttcgccg ggctcgacag tctggtgctg agtcccggcg
     2161 tgccgctcac ccatccggaa ccgcactgga cggtggtgaa ggccaaggcc gccgggattg
     2221 aaatcatcgg cgacacggaa gtgttccagc gggaagttgc gggcacgggc gcgcggattg
     2281 ttgcgattac cgggaccaat ggcaaatcca ccaccacggc gctcattggc catatctttc
     2341 aatcactggg ccgcgatgtt gacgtgggcg gaaatatcgg cactgcggtt ttcctgatgc
     2401 ggccgccggt ggaaggccgc atctatgtgc ttgagctttc ctcttttcag attgacctga
     2461 tgccggggct gagaccggaa gtgggcattc tgaccaacct gacgccggac catctcgacc
     2521 gtcacggcag catggacaat tatgcggcaa tcaagtcccg gatgttcctg aagcaggacg
     2581 gcaatgacac ggcggtgatt ggcgtggatg acgaatggtg cgccggcatt gccatcggaa
     2641 ttgcgaatgg acagaagc
//
LOCUS       DAORVN010000265         2653 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_36686_length_2653_cov_3.062741, whole genome shotgun sequence.
ACCESSION   DAORVN010000265 DAORVN010000000
VERSION     DAORVN010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2653
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36686_length_2653_cov_3.062741"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            190..966
                     /locus_tag="PKV23_06170"
     CDS             190..966
                     /locus_tag="PKV23_06170"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018391429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HQX85056.1"
                     /translation="MTSRRTRIYEGKAKILYEGPEPGTVIVHFKDDATAFDAQKKAVI
                     EGKGVLNQRISEYIFSRLNEVGIPTHFMRSLNLREQLVREVEIIPLEVIVRNVAAGSL
                     AKRLGLEEGTSLPRSIIEFCYKNDALHDPMVSEEHITAFGWAAPQEIDDIMHMSIRIN
                     DFLTGLFLGIGIKLIDFKIEFGRLYENDMMRIVLADEISPDSCRLWDTKTNDKMDKDR
                     FRRDMGGLVEAYQEVAKRLGIISENEKPERAKPKLVKSDT"
     gene            963..1205
                     /gene="purS"
                     /locus_tag="PKV23_06175"
     CDS             963..1205
                     /gene="purS"
                     /locus_tag="PKV23_06175"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007606068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HQX85057.1"
                     /translation="MMKAKIKVTLKKGVLDPQGKAIEGALGHLGFAGVEHVRQGKYFE
                     IDVAETDRSKAMTEVRSMCEKLIANTVIENYDIELA"
     gene            1202..1864
                     /gene="purQ"
                     /locus_tag="PKV23_06180"
     CDS             1202..1864
                     /gene="purQ"
                     /locus_tag="PKV23_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003531403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HQX85058.1"
                     /translation="MKSAVVVFPGINRERDMMLALEAVTGVAPVAVWHTDPQLPKVDL
                     VVVPGGFSYGDYLRCGAIAARSPIMSDIRAKAAKGLKVLGVCNGFQIVAEAGLLPGAL
                     VRNAHLHFVCREVKLAVENNSTFFTNRMKKGAVVSCPVAHGEGNYICDEETLKALEGE
                     GRVVFRYTQGTNPNGAMNDIAGIVNKAGNVIGMMPHPENMIEPLHGKTDCRPLFESLL
                     AA"
     gene            1904..>2653
                     /locus_tag="PKV23_06185"
     CDS             1904..>2653
                     /locus_tag="PKV23_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85059.1"
                     /translation="MSDYYDLGPYTRPVTTTSKEAQIWFDRGLNWCYGYNHEEAVRCF
                     KKAAKHDPNCAMAQWGIAYAAGPNYNKQWVAFDPVDLKSSLGLACAATKQALKLLDQA
                     SPVEQALIRPLAKRYPLNEPGNVTPIWNDDYAAAMREVYLAHKDDLDVAALFAEAIMN
                     RTPWLLWDIKTGKPAEGADTAEAIAVLERAMATASGMKHPGILHMYIHLMEMSPHPER
                     ALRAGDALCSLVPDAGHLIHMPTHIDVLCGNY"
BASE COUNT          634 a          793 c          696 g          530 t
ORIGIN      
        1 caatggagat agctagtcgc atggcccaaa tcaactattc aaaaccattg tgcaatgcaa
       61 taagttgaag tagaagcccc ggcaattggc gagaaccgtc gccatattgt cgaaacatgt
      121 gaactataag taccgcccgc tggttttgtg ctaaaagggt ggataagggt tttgatgagg
      181 atattgcata tgaccagcag aagaacccgc atttacgaag gcaaagccaa gattctctac
      241 gaaggccccg agcccggcac ggttatcgtg catttcaagg atgatgcaac cgccttcgac
      301 gcccaaaaga aagcagtgat tgaaggcaag ggcgtgctca accagcgcat ctccgaatac
      361 attttttcgc gcctgaatga agtcggcatt ccaacccatt tcatgcggtc gctgaatttg
      421 cgtgagcagc tcgtgcgcga agttgaaatc attccgctgg aagtgattgt gcgcaatgtg
      481 gcggcaggct cactggccaa gcgccttggc cttgaggaag gcacatccct gccgcgctcc
      541 atcattgaat tctgctacaa gaacgacgcg ctgcatgacc ccatggtttc tgaagagcat
      601 atcactgctt ttggctgggc cgccccccag gaaatcgacg acatcatgca catgtcgatt
      661 cgcatcaacg acttcctcac cggcctgttc ctcgggattg gcatcaagct gatcgatttc
      721 aagatcgagt tcggcaggct ttacgagaac gacatgatgc gcattgtcct ggccgatgag
      781 atcagccccg actcatgccg tctctgggac accaagacca acgacaagat ggacaaggac
      841 cgtttccgcc gcgatatggg tggtctggtc gaagcctacc aggaagtggc caagcgcttg
      901 ggaattataa gcgagaacga aaaacctgaa cgcgccaagc ccaaacttgt gaagagcgat
      961 acctgatgaa agccaaaata aaagtcaccc tgaaaaaagg tgtgctggac ccgcaaggca
     1021 aagccatcga aggcgccctg ggccatctcg gctttgccgg cgtcgaacat gtgcgtcagg
     1081 gcaagtattt cgaaattgac gtcgccgaaa ccgacaggtc aaaggccatg accgaggtca
     1141 ggtccatgtg cgaaaagctg atcgccaaca ccgttatcga gaactatgat atagagcttg
     1201 catgaaatcc gccgtcgtcg ttttccctgg catcaaccgc gaacgtgaca tgatgcttgc
     1261 ccttgaggcg gtcaccggcg ttgccccagt tgctgtctgg cacaccgatc cgcaactgcc
     1321 gaaagttgat cttgtagtcg ttcccggcgg tttttcctat ggcgactatc tccgctgcgg
     1381 cgccatcgcc gcgcgttcgc ccatcatgag cgatattcgc gccaaggccg ccaaaggcct
     1441 caaggttctg ggcgtctgca acgggttcca gatcgtcgcc gaggcaggcc tcctgcccgg
     1501 tgcgctggtg cgcaatgccc accttcactt tgtttgccgc gaggtgaaac tggccgttga
     1561 aaacaactcc accttcttca ccaaccgcat gaagaaaggc gccgtcgtct cctgccccgt
     1621 ggcccacggc gagggcaact atatctgcga tgaagaaacc ttgaaggccc tggaaggcga
     1681 aggccgcgtc gtcttccgct acacccaggg caccaatcct aacggcgcca tgaacgacat
     1741 cgccggcatc gtgaacaaag caggcaatgt tattggcatg atgccccatc cggaaaacat
     1801 gatcgagccg ctccacggca aaaccgattg ccgcccgctg tttgaaagcc tgctagccgc
     1861 ctgatttcca ccgtgatagg ttcctttcgc gtggaggaac atcatgtcag attattatga
     1921 tctcggcccc tacacccgtc ctgtcaccac gacttcaaag gaagcccaga tttggttcga
     1981 tcgtggcctc aactggtgtt atggctacaa tcatgaagag gcggtgcgct gtttcaagaa
     2041 agccgccaag cacgatccca actgcgccat ggcccaatgg ggcatcgctt atgcggcggg
     2101 ccccaactac aacaagcaat gggttgcctt tgatccggta gacctgaaaa gttcactcgg
     2161 ccttgcctgc gccgccacca agcaggcgct gaagctcctt gatcaggcaa gcccggttga
     2221 gcaggccctc atacgccccc ttgccaagcg ctatccgtta aacgaacctg gcaatgtcac
     2281 gcccatctgg aacgacgatt atgccgccgc tatgcgcgag gtctacctcg cccacaagga
     2341 cgatctcgac gtggcagccc tcttcgccga ggcgatcatg aaccgcaccc cctggctgct
     2401 ctgggatatc aagacgggaa aaccggctga aggagccgac acggcagaag ccattgccgt
     2461 tttggaacgc gccatggcta ccgcaagcgg catgaaacac ccgggcatcc tccacatgta
     2521 catccacctc atggaaatgt cgccccaccc ggaacgcgca ctgcgcgccg gagacgcgct
     2581 atgcagcctc gtccccgatg caggccacct cattcacatg ccaacccaca tcgacgttct
     2641 ctgcggcaac tat
//
LOCUS       DAORVN010000266         2652 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_36718_length_2652_cov_2.432422, whole genome shotgun sequence.
ACCESSION   DAORVN010000266 DAORVN010000000
VERSION     DAORVN010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2652
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36718_length_2652_cov_2.432422"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..945
                     /locus_tag="PKV23_06190"
     CDS             <1..945
                     /locus_tag="PKV23_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008877985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HQX85060.1"
                     /translation="GAQAKFTFALVPKNMNNPFFDQARDGCKKAEAESNGAFECMYIG
                     PGEHGGGDEQVQIVQDLVAKKVDGIAVAVANAAAMTVALQAAKDAGIPVLTWDSDMLP
                     ENKDLRLAYIGTHNYEIGTNLAALAMKIKPNGGTVCIQSGGAAAANHNERMQGIRDVL
                     SGTKSAASPGDRLTGQNGWTEIEGCPLYTNDDFPLSVQQFEDIMAKNPKLDAFIPTGG
                     FPQFVPDANRAAVEKYKGMIADKSLALVVADTLPVQIDQMKEGYSLGQVGQRPFEMGY
                     MTMKAFADMKDGKAAPADPSYTGLDVCTPETADTCIAK"
     gene            1005..1754
                     /locus_tag="PKV23_06195"
     CDS             1005..1754
                     /locus_tag="PKV23_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008839681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQX85061.1"
                     /translation="MSKMFSLENRVALVTGASRGLGLAMAKALKENGAHVIINARDAA
                     ALATAAKPIGAQPMAFDVSDAAVSRKALEDIEREHGHLDILVNNAGIQHRKPLVEWED
                     ADFDRVISVNLSSCFRLARDAARLMLPRKFGRIINTGSVAAILGRPTIHAYVAAKAGL
                     HGLTRSMAAELGRHGITINALAPGYFATELNTALLEDKAFTAWVEARTPAGRWAKPDE
                     LGGAAVFLASDAAAYVNGHVLAVDGGLSTSL"
     gene            complement(1755..>2652)
                     /locus_tag="PKV23_06200"
     CDS             complement(1755..>2652)
                     /locus_tag="PKV23_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HQX85062.1"
                     /translation="LAQLDKLARKFHWGDFLRPDVPVSVEVSSRKSRIYHASAAAQRI
                     ETAIREELGAPISPDAELCVMARFEDNLCTISLDTSGSSLYKRGYKEAVAKAPMRETM
                     ASLFLRQCGYAGHETVVDPMCGSGTFVIEAAEIAAKLNPGRSRRFAFEQLTSFDEGAW
                     QRLRNASHGIKPALHFFGSDRDAGAIQMSRANAERAGVSAFTVFQNHAVSNITPPPGR
                     PGLVIVNPPYGTRIGDKAPLVTLYAALGKTLLARFSGWRVGLITTDTSLAKATGLPFA
                     PPGRPASHGGLKVLLFQTGRLT"
BASE COUNT          508 a          854 c          821 g          469 t
ORIGIN      
        1 ggggcccagg cgaagttcac cttcgcgctg gttcccaaga acatgaacaa cccgttcttc
       61 gaccaggccc gtgatggctg caagaaggcc gaggctgaat ccaacggcgc ctttgaatgc
      121 atgtatatcg gccccggcga gcacggcggc ggcgacgaac aggtccagat cgtgcaggat
      181 ctcgtggcca agaaggttga tggcattgcc gtggccgtgg ccaacgccgc tgccatgacg
      241 gtggcccttc aggcggcaaa ggatgccggc attccggttc tgacctggga ttccgacatg
      301 ctgcccgaga acaaggacct gcgtctggcc tatataggca cccacaacta tgagatcggc
      361 accaacctcg cggcactcgc catgaagatc aagccgaacg gcggcactgt ctgcatccag
      421 tcgggcggcg ctgcggcggc caaccacaac gagcgcatgc agggcatccg cgacgtgctc
      481 tcgggcacga agtcggccgc ctcgcctggc gaccgcctga ccggccagaa cggctggacg
      541 gaaatcgagg gttgcccgct ctacaccaac gatgacttcc cgctctcggt gcagcagttc
      601 gaggacatca tggccaagaa ccccaagctg gatgccttca tccccaccgg cggcttcccg
      661 cagtttgttc ccgatgcaaa ccgcgccgct gtcgagaagt acaagggcat gatcgccgac
      721 aagtcgctgg cccttgtggt cgccgacacc ttgccggtgc agatcgacca gatgaaggaa
      781 ggctattccc tcggtcaggt gggccagcgc ccgtttgaaa tgggctacat gaccatgaag
      841 gcctttgccg acatgaagga tggcaaggcc gcaccggctg atccgtccta cactggtctc
      901 gatgtctgca cgcccgaaac ggccgatacc tgcatcgcga agtagaacat taacatgtag
      961 ttgaaagggc ggggcgctcc cccgcctttt tattggagga agtgatgtcg aaaatgttct
     1021 cactggaaaa tcgcgtggca ctggtcaccg gcgcttcgcg cggactgggc ctcgccatgg
     1081 caaaggccct gaaggaaaac ggcgcccatg tcatcatcaa cgcccgcgat gcggcagccc
     1141 ttgccacggc ggcaaaaccc atcggcgcgc aacccatggc cttcgatgtg agcgatgcgg
     1201 ccgtttcgcg caaagccctt gaagacattg agcgggaaca cggccatctc gacatcctgg
     1261 tcaacaatgc cggcatccag caccgcaagc cccttgtcga atgggaagat gcggattttg
     1321 atcgcgttat cagcgtcaat ctcagctcct gctttcgcct cgcgagggat gcggcccgcc
     1381 tcatgctgcc gcggaaattc gggcgcatca tcaataccgg atctgtcgcc gccattcttg
     1441 gccgccccac catccacgcc tatgtggccg ccaaggcggg cctgcatggc ctgacccgct
     1501 ccatggcggc ggaactgggc cgccacggca tcaccatcaa tgccctggcg cccggctatt
     1561 tcgccaccga actcaacacc gcgctcctcg aagacaaggc gttcaccgca tgggttgaag
     1621 cgcggacccc ggcggggcgc tgggcaaaac cggatgaact ggggggagcc gcggtctttc
     1681 tcgcctccga tgccgcggcc tacgtcaacg gccacgtgct ggcggtcgat ggtgggcttt
     1741 cgacgtcgct gtgattacgt caatcgcccg gtctggaaca gcagcacctt gagcccgcca
     1801 tgggacgcag gccgccccgg cggcgcgaag ggcaggccag tggctttggc caatgacgtg
     1861 tcggtggtga tcagccccac gcgccagccg gaaaatcgcg ccagaagcgt tttccccagt
     1921 gcggcataga gggttaccag cggcgccttg tcgccgatcc gtgtgccata gggcgggttg
     1981 acgatcacca ggccgggccg ccccggcggc ggcgtgatgt tgctcaccgc atgattctgg
     2041 aacaccgtga aggccgagac cccggcccgc tccgcattgg cccggctcat ctggattgca
     2101 cccgcgtcac ggtcgctgcc gaaaaaatgc agggcgggct ttatgccgtg ggacgcattg
     2161 cgcaggcgct gccatgcgcc ttcatcgaag cttgtcagtt gctcgaaagc aaagcgccgt
     2221 gaccggcccg gattcaactt cgcggcgatt tccgccgctt caatgacgaa ggtgccggag
     2281 ccgcacatgg gatcgaccac ggtttcgtgg ccagcatatc cgcattgcct caggaagagc
     2341 gaggccatgg tttcccgcat cggcgccttg gccacggctt ccttgtaccc gcgcttgtag
     2401 agcgaagagc ccgaggtatc gaggctgatc gtgcacagat tgtcttcgaa gcgcgccatg
     2461 acgcaaagct ccgcgtctgg ggagatgggg gcgccgagtt cttcacggat cgcggtttcg
     2521 atccgctgcg ccgccgcact cgcatggtag atgcgcgact ttctgctgga gacttcgaca
     2581 ctgaccggca catcgggccg caggaaatcc ccccagtgaa atttgcgggc gagcttgtcg
     2641 agctgcgcca gg
//
LOCUS       DAORVN010000267         2649 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_36793_length_2649_cov_3.156901, whole genome shotgun sequence.
ACCESSION   DAORVN010000267 DAORVN010000000
VERSION     DAORVN010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2649
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36793_length_2649_cov_3.156901"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="PKV23_06205"
     CDS             <1..224
                     /locus_tag="PKV23_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006330665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85063.1"
                     /translation="EVDAPLDDHNVERFCNLLDAMLERTETRFLIITHHELTMSRMHR
                     LFGVTMMERGVSQLVSVNLEEAEAMAEAS"
     gene            245..1075
                     /locus_tag="PKV23_06210"
     CDS             245..1075
                     /locus_tag="PKV23_06210"
                     /inference="COORDINATES: protein motif:HMM:NF016202.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HQX85064.1"
                     /translation="MLKTLPKPQQRSALRMALGKTWFTAKRRMQWHLPGKRYATSRSS
                     TALPHHHASHRTPLLRPLKGVDMWLQHNKVASLKVAASCIDGLLLRPGETFSFWRLVG
                     KPTARRGFRTGLVLRNGQISQGIGGGLCQMTNLIYWMALHTELSITERWRHGFDVFPD
                     EGRSQPFASGATCSYNYIDLQISNRTANTYQLRVWVEGDYLNGEWRAEAHPRHTYEIV
                     ERDHAIRTEWWGGYSRHNSLHRIARDHSTGAECGEEFITENHAIMMYAPLLKNLETSP
                     "
     gene            1072..1668
                     /locus_tag="PKV23_06215"
     CDS             1072..1668
                     /locus_tag="PKV23_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006934150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HQX85065.1"
                     /translation="MNIVFDIGNVLLRWDPRFLFTQYFENEEEMEWFLAHVCNHEWNL
                     EQDRGRSFAEAVKLATAQYPDHAEAIAAYDTQWHQMIPHAIDGTVEIFKELVERDAPV
                     YAITNWNGDKFREAKQRFPFLYEFRDIVVSGDEKLLKPDPAIYQLLLGRNSLEAGDCL
                     FIDDSLKNVKGAEAIGMKAHHFTTPEALRNMLVELELL"
     gene            complement(1665..>2649)
                     /locus_tag="PKV23_06220"
     CDS             complement(1665..>2649)
                     /locus_tag="PKV23_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007007125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HQX85066.1"
                     /translation="DPAPEAHGVVVRVMATGVCRSDWHGWVGHDADIRLPHVPGHELA
                     GFVEAVGRDVRNWKKGDRVTVPFVSGCGSCPQCHTGNHQICDNQFQPGFTHWGSFAEY
                     VSIHYADINLVRLPETIDFATAASLGCRFVTSFRAIVDQGRVSAGQWVAVHGCGGVGL
                     SAIMIANALGAQVVAIDIADQKLELAKSVGAAATVNAGKTANVVEAVMEITGGGAHVS
                     VDALGHATTCFNSISNLRKRGRHIQVGLMLAEHGTPPIPMGKVIAHELEIYGSHGMQA
                     HRYPDMLAMIQAGKLSPEKLIGKTISLEQSIDALMNMDKFEAAGVTVVTKF"
BASE COUNT          527 a          809 c          816 g          497 t
ORIGIN      
        1 acgaagtcga cgcgccgctc gacgaccaca atgtggagcg gttctgcaac ctgctcgacg
       61 ccatgctgga acgcacggaa acgcgcttcc tgatcatcac ccaccacgaa ctcaccatgt
      121 cgcgcatgca ccggctgttc ggcgtcacca tgatggagcg cggcgtgtcg cagctcgtct
      181 cggtaaacct tgaagaggcg gaagcgatgg cggaggccag ctagggcttc acgcgagggg
      241 aaccatgttg aagacgctcc ccaaaccaca gcagcgttcg gccctgcgca tggccttggg
      301 gaaaacctgg ttcaccgcca agcggcggat gcagtggcat ctgccgggca agcgttatgc
      361 gaccagccgg agttccacgg cgcttccaca tcaccatgcc agccaccgca caccgctctt
      421 gcggccgctc aagggtgtgg acatgtggct gcagcacaac aaggtggcga gcctgaaggt
      481 ggccgcctcc tgcattgacg ggcttctgct cagacccggc gagacctttt ccttctggcg
      541 gctggtgggg aaacccacgg cgcggcgcgg cttcaggacc ggtctggtgc tgcgaaacgg
      601 ccagatttcg caggggatcg gcggcgggct ctgccagatg accaatctca tctactggat
      661 ggcgctgcac accgaacttt ccatcaccga gcgctggcgc catggctttg acgtgtttcc
      721 cgacgagggg cgcagccagc cttttgcctc gggcgccacc tgctcctaca actacatcga
      781 cctgcagatc agcaaccgga cggcaaatac ctatcagttg cgggtgtggg tggagggcga
      841 ctacctcaat ggcgaatggc gggccgaagc gcatccgcgc cacacctatg agattgtcga
      901 gcgcgaccat gccatccgga ccgaatggtg gggcggttac agccggcaca actcgcttca
      961 ccgtattgcg cgcgaccaca gtaccggggc agaatgcggt gaagaattca tcaccgagaa
     1021 ccacgccatc atgatgtatg caccgctttt gaaaaacctg gaaacctcac catgaacatc
     1081 gtcttcgata tcggaaatgt ccttctgcgc tgggatccgc gcttcctgtt cacccagtat
     1141 ttcgagaatg aagaggagat ggagtggttc ctcgcccatg tctgcaatca tgaatggaac
     1201 ctggagcagg accgggggcg gagctttgcc gaggcagtga agctcgccac ggcgcagtat
     1261 cccgaccatg cggaggcgat tgccgcctat gacacgcagt ggcatcagat gatcccccac
     1321 gccattgacg gaacggtgga gatcttcaag gaactggtgg agcgcgacgc gccggtctat
     1381 gccatcacca actggaacgg cgacaagttc cgcgaggcca agcagcgctt tcccttcctc
     1441 tatgaattcc gcgacatcgt ggtgtcaggc gatgagaaac tgctgaagcc tgatccggca
     1501 atctatcaat tgctgctcgg gcgcaacagc cttgaggcgg gcgattgcct gttcatcgat
     1561 gacagcctga agaatgtgaa aggcgctgaa gccatcggca tgaaggcgca tcacttcacc
     1621 acgccggaag ccttgcggaa catgctggtg gaacttgagc tgctttagaa cttcgtcacg
     1681 acggtgacgc ctgccgcttc gaacttgtcc atgttcatca gcgcgtcgat tgactgttcg
     1741 aggcttatgg ttttgccgat aagtttttcg ggcgacagtt tccccgcctg gatcatggcc
     1801 agcatatcgg gatagcggtg ggcctgcatg ccgtggctgc cgtaaatctc cagttcgtgg
     1861 gcgatgacct tgcccatggg aatcggcggc gtgccgtgtt cggccagcat gagccccacc
     1921 tggatatggc gcccgcgctt gcgcaggttg ctgatcgagt tgaagcatgt ggtggcatgg
     1981 cccagggcat ccaccgagac atgggccccg cccccggtga tctccatgac ggcctcgacg
     2041 acattggccg tcttgccggc attcaccgtg gcggcagcgc ccactgattt tgccagctcg
     2101 agtttctggt cggcaatgtc gatggcgacc acctgggctc ccagagcatt ggcgatcatg
     2161 atggcggaca ggcccacgcc accgcagcca tggaccgcga cccattgccc ggcggagacc
     2221 ctgccctgat cgacgatggc gcggaacgag gtgacaaaac ggcagcccag gcttgcggca
     2281 gtggcgaaat cgatggtttc cggcaggcgg acgagattta tatcggcgta gtggatggac
     2341 acatattcgg caaaggagcc ccaatgggtg aagccgggct gaaactggtt gtcgcagatc
     2401 tggtgattgc ccgtgtggca ctgggggcat gaaccgcagc cgctgacgaa gggcacggtc
     2461 acccggtcgc cctttttcca gttcctcaca tcccggccca cggcctcgac aaatcccgcc
     2521 agttcgtggc ccggcacatg gggaagcctg atatcggcgt catggcccac ccagccgtgc
     2581 cagtcactgc ggcagacgcc ggtggccatg accctgacga caaccccgtg cgcttcgggc
     2641 gcgggatcg
//
LOCUS       DAORVN010000268         2647 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_36829_length_2647_cov_2.883488, whole genome shotgun sequence.
ACCESSION   DAORVN010000268 DAORVN010000000
VERSION     DAORVN010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2647)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2647)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2647
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36829_length_2647_cov_2.883488"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..838)
                     /locus_tag="PKV23_06225"
     CDS             complement(<1..838)
                     /locus_tag="PKV23_06225"
                     /inference="COORDINATES: protein motif:HMM:NF015763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85067.1"
                     /translation="MMRLLRALLMVFILAGVFAAPAMAQTAGEPSSEIKIDKKKLLVQ
                     PRNADGSPIMVSFWEDPVLWVREKQQAYYGSMSGTLRQIRTESPYAAAWTLMLLSFGY
                     GIFHAAGPGHGKTVISAWLLATENELKRGIVIAFLSSIIQAFVAILLVSVLFLVVASA
                     SSAARDVAGVLESASYAMIGALGLYLIWTALRPKAHHDHHEHAHHGHAHHHHDHVDDE
                     HCGHAHVAEAKQVRGEWSLTKAFSLAFAVGIRPCTGAILVLILANALGIYWAGVASTL
                     VMG"
     gene            complement(835..1503)
                     /locus_tag="PKV23_06230"
     CDS             complement(835..1503)
                     /locus_tag="PKV23_06230"
                     /inference="COORDINATES: protein motif:HMM:NF017985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1007 family protein"
                     /protein_id="HQX85068.1"
                     /translation="MAARRNLYLVMAAMMAGFAGTEARAHPHVWIEMQSDVVFTEDGL
                     IKGVNLEWTFDDAYTQMALDGLDVDGDGLYSQSELAPLTKENIESLKDYEYFTVMRAN
                     GEQQKIGPVTDFGQIWSNSKLTLHLQVPLEKPVDPRTQEFVLKVYDPEFFIAIDYAGD
                     DPVDVVGNMPEACKLVVKPVPTDAELDQTRTMLSTKGADWKPENPEDFGAMFAQAVSI
                     ACKS"
     gene            1644..2570
                     /locus_tag="PKV23_06235"
     CDS             1644..2570
                     /locus_tag="PKV23_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006310430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HQX85069.1"
                     /translation="MALQKTMGAREWALLLILSVLWGGSFFFIGVAVKELPPLTIMML
                     RVGLAALTLHVVIRAMGQWMPRDPRIWLAFAGMGLLNNAIPQTLIVWGQTQIPSGLAS
                     ILSATTPLFGVLVAHFFTTDERMTGKKLAGVIVGFVGAAVMVGPAALQGLGTNVWAQV
                     AILLASLFYGISGVYGRRFKQMGVTPMMTATGQLTASTLMLTPVALIMDQSWTLTHPS
                     LAAWAAIAGLAILSTALAYLIFFRILASSGATNLLLVTFLIPVSAVLLGFLFLGEQLE
                     PRHFAGMGLIALGLAAIDGRLLALRRPGVRTP"
BASE COUNT          491 a          786 c          824 g          546 t
ORIGIN      
        1 tgcccatgac cagggtcgaa gccacgcccg cccagtaaat tcccagggca ttggccagga
       61 tcagcaccag aatggcccca gtgcagggcc tgatgccgac ggcgaaggcc agcgaaaatg
      121 ccttggtgag ggaccattcg ccccgcacct gtttggcttc cgccacatgg gcatggccgc
      181 aatgctcgtc gtcgacgtga tcatggtggt gatgggcgtg gccgtgatgc gcgtgctcat
      241 ggtgatcgtg atgggctttg ggacgcaatg ctgtccagat cagatagagg cccaaggcgc
      301 cgatcatggc gtagctggcg ctttcgagga cgcctgccac atcgcgtgcc gcactgctgg
      361 cgcttgccac cacgagaaac aaaacgctca ccagcaaaat ggcaacgaag gcctgaatga
      421 tggaactgag gaaggcgatg acaatgccgc gcttcaattc attttccgtg gcgagcagcc
      481 aggcggaaat caccgtcttg ccatggccgg ggccggcggc gtgaaatatg ccgtaaccga
      541 agctcaacag catgagggtc caggcagcag cgtaagggct ctcagtcctg atctgccgga
      601 gggtgcccga catggagcca taataggcct gctgtttttc gcgcacccag agcacgggat
      661 cttcccagaa cgacaccatg atgggcgagc cgtcggcatt gcgcggctgg acaagaagtt
      721 tcttcttgtc gatcttgatt tcgcttgagg gttcgcctgc ggtttgcgcc atggccgggg
      781 ctgcaaagac tcccgcgagg ataaaaacca taagcaacgc gcgcagcaga cgcatcatga
      841 tttgcatgcg atggacacgg cctgggcaaa catggcgccg aagtcttccg gattttccgg
      901 cttccagtcc gcacccttgg tggagagcat ggtgcgggtc tggtccagtt ccgcatcggt
      961 gggcacgggc ttcaccacca gcttgcaggc ttccggcata ttgcccacca catcgacggg
     1021 atcatccccg gcatagtcga tggcgatgaa gaattccggg tcgtaaacct tgagcacgaa
     1081 ttcctgggtc ctgggatcca cgggcttttc caggggaacc tgaagatgaa gcgtcagctt
     1141 gctgttgctc cagatctgcc cgaagtcggt gaccgggccg attttctgct gctcgccatt
     1201 ggcgcgcatg acggtgaaat attcatagtc cttgagcgac tcgatattct ccttggtaag
     1261 cggcgccagt tcgctctggc tgtaaaggcc gtcgccatcc acatcgaggc catcgagggc
     1321 catctgggta taggcgtcgt caaaggtcca ttccaggttg acgcccttga tcaggccgtc
     1381 ctcggtgaac accacatcgg actgcatctc gatccagaca tgggggtggg cccgggcctc
     1441 ggtccctgcg aaaccggcca tcattgcagc catgacaaga taaaggttgc ggcgcgccgc
     1501 catccgtgaa aactccgctc caaggcccgc atagtgaacg tatgcggcaa aaaagtgaaa
     1561 ttaagttttg gcaacatcat gaatcgggtg ggcggcggaa tgtcccactg gcatggtcgg
     1621 ttgcgcaacg gaaatgggaa gatatggcat tgcaaaagac catgggggcc agggaatggg
     1681 ccctgctcct catcctttcg gtgctgtggg gcggttcgtt cttcttcatc ggggtcgccg
     1741 tcaaggaact gccgccgctc accatcatga tgctgcgggt gggtctggcc gccctgaccc
     1801 tgcatgtggt catccgcgcc atggggcaat ggatgccgcg cgacccccgg atatggctgg
     1861 cctttgccgg catggggctt ctcaacaatg ccattccgca gacgctcatt gtctgggggc
     1921 agacgcagat ccccagcggg ctggcctcga tcctgagcgc caccacgccg ctgttcggcg
     1981 ttctcgtggc gcatttcttc acgacagacg aacgcatgac gggcaagaag ctggcggggg
     2041 tcatcgtcgg atttgtgggc gccgcggtca tggtgggccc tgcggcgctg caggggctcg
     2101 ggaccaatgt gtgggcgcaa gtggccattc tcctggcttc actgttttat ggaatctcgg
     2161 gcgtctatgg gcggcgcttc aagcagatgg gggtgactcc catgatgacg gccacggggc
     2221 agctcacggc ttcaaccctg atgctcacgc ccgttgccct gatcatggac cagtcctgga
     2281 cgcttaccca tcccagcctt gcggcgtggg ccgccattgc cgggcttgcc attctttcaa
     2341 cggcgctggc ctatctcatc ttcttccgca tccttgcctc gtcgggagcc accaacctgc
     2401 tgctggtcac tttcctgatc ccggtcagtg cggtgctgct tggcttcctg tttctcggcg
     2461 agcagctgga gccccggcat ttcgccggca tggggcttat cgcgctgggc ctggcggcca
     2521 tcgacgggcg gctgctggcg ctgcgccggc ctggggtcag gaccccttaa tctgaaagtt
     2581 gcgaaccgca gctttgcaag gcgagacgga cattcagttc gactcctcga gagtccgctc
     2641 cgaatcc
//
LOCUS       DAORVN010000269         2644 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_36896_length_2644_cov_2.933951, whole genome shotgun sequence.
ACCESSION   DAORVN010000269 DAORVN010000000
VERSION     DAORVN010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2644)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2644)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2644
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36896_length_2644_cov_2.933951"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..894
                     /locus_tag="PKV23_06240"
     CDS             <1..894
                     /locus_tag="PKV23_06240"
                     /inference="COORDINATES: protein motif:HMM:TIGR01620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HQX85070.1"
                     /translation="LVVLPVVAVRRSRRFRWGFVLVSALAALLSLWAGLAITTLVEDF
                     FARSASLGWLALGVAGLAGLAALAIVIREIWGLSRLRRIEHVQELSARALNLDEAAAA
                     AETIASLEGIYGGRSDTALGLKTFHTHKNDIMDPRDRIRLFDRLVISPLDEESHRIIA
                     RRARRVTLLTTVTPAAALDIVFVAAQNLRMLRELAELYGGRPSTLSTLKLARMVVSHL
                     AVTGGLALSDNLIQHVVGKGLLGRLSARFGEGAVNGILTSRIGLAARDVCRPVPQTES
                     TKETLGTLLRELVKLNDSRAP"
     gene            984..1490
                     /locus_tag="PKV23_06245"
     CDS             984..1490
                     /locus_tag="PKV23_06245"
                     /inference="COORDINATES: protein motif:HMM:NF024600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85071.1"
                     /translation="MQKSTKIIIVAVAGLAVIGGAAFAQKGWRHGFGGHGLMGFAQMS
                     ERYDANKDGKISQEEIDANRTSWLGEFDADKSGSLALSEFQNLWLKARNQQMVREFQQ
                     FDRDGNGQVTLDEYKLPLSKIVAEMDRDKDNALSGDELSPRRHWGKKHHERMRGGDDD
                     DKDEQDTQ"
     gene            1676..>2644
                     /locus_tag="PKV23_06250"
     CDS             1676..>2644
                     /locus_tag="PKV23_06250"
                     /inference="COORDINATES: protein motif:HMM:NF003715.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HQX85072.1"
                     /translation="MDTIWHVNLALFVGAALMIGGILSSLLSARFGAPLLFIFLSIGM
                     LAGEDGPLGIVFNDYRLTYTIGSIALAVILFDGGLRTRLVVFQRSLLPAGILATLGVV
                     ATAGAIGLVASWLLGITLLEGFLVGAIVSSTDAAAVFYLLRTGGLQLKRRIGATLEIE
                     SGTNDPASAFLTILLVQLILAGNGDGYAGILQFLLTQILLGTAIGIGGGFVLVWALGN
                     LRLPPGLRPLFVVGGAIAIFALTSLLSGSGFLAAYLAGLVLGNREARGFPAILSFHDA
                     ATWLAQIAMFLILGLLVTPSKLLDYFLPALAIAAFLIVVARPAAVFL"
BASE COUNT          474 a          810 c          761 g          599 t
ORIGIN      
        1 cttgtggtgt tgcccgtcgt cgctgtgcgc cggtcccgga ggttccgctg gggcttcgtg
       61 cttgtttccg ccctcgccgc tctcctcagc ttgtgggccg gccttgccat aaccacgctc
      121 gttgaggatt tctttgcccg ctcggcttcg ctcggctggc ttgcccttgg tgttgcgggc
      181 cttgcaggct tggcggccct cgcaatcgtt atccgcgaaa tttgggggct atcacgcctc
      241 aggcgcatag aacatgtcca ggagctgtcg gcccgcgcct tgaacctcga tgaggccgca
      301 gccgcagccg aaaccatcgc ttcgctggag ggaatctatg gtggtcgctc cgacacggct
      361 ctcggcctga aaaccttcca cacccataaa aacgacatca tggatccccg tgaccgcata
      421 aggctctttg accgtctcgt cataagcccg cttgatgaag agtcccaccg catcattgca
      481 aggcgggccc ggcgcgtgac cctgctcacc acggttacac cggcggcggc cctcgacatt
      541 gtctttgtcg ccgcccagaa cctgcgcatg ttgcgcgagc tcgctgaact ctacggcggc
      601 aggccctcaa cgctttcaac cctgaaattg gcgcgcatgg tggtgtccca tctcgctgta
      661 accggcgggc tggcactttc cgacaatctc attcagcatg tcgttggcaa aggcctgctg
      721 ggaaggctct cggcccgatt tggcgaaggc gccgttaacg gtattctcac ctcccgcatc
      781 ggccttgcag cgcgcgacgt ttgccgcccc gttccccaga cagaatcgac caaggaaacc
      841 ctgggcaccc ttctgcggga actggtgaaa ctcaatgatt ccagggcgcc ctaacattaa
      901 caatatttta gcccatgttg cgacggaatg ggcagccgcg ggcagtccta actccatgca
      961 gatggaaatt taaggagaaa cccatgcaga aatccacgaa gataatcatc gttgcggttg
     1021 ccggccttgc agtgattggc ggcgcggcct tcgcccagaa gggctggagg catggcttcg
     1081 gcggccatgg ccttatgggc ttcgcgcaga tgtcggagcg ctacgatgcc aacaaggatg
     1141 gcaagatttc ccaggaggaa attgatgcca accgcaccag ttggctcggc gagttcgatg
     1201 ctgacaagtc cggctctttg gcgctttccg aattccagaa cctttggttg aaggcccgca
     1261 atcagcagat ggtccgcgag ttccagcaat tcgaccggga tggcaacggc caggtcacct
     1321 tggacgaata caagctcccg ctctccaaaa tcgtggccga aatggaccgc gacaaggaca
     1381 acgcgctttc cggtgacgag ctttcgcccc ggcgccactg gggcaaaaag caccatgaga
     1441 gaatgcgcgg cggcgatgac gacgacaagg atgagcaaga tacccagtaa gtgatttcct
     1501 ccggccgccc atcgggcggc cggatcgttc ttggcaatat aattcaaaag aacgcatgtc
     1561 cagccttgaa gaccggttca gccgtatcga tgcgcaggcg cacccccatc gcgaagtttc
     1621 ccccggcgcg atctgatcga atagacaatc gacctattga tgttaacttg ctgccatgga
     1681 taccatttgg catgtcaatc tagcattgtt cgtcggtgcg gccctgatga ttggcggcat
     1741 tctgtcgagt ttgctctcgg cacggtttgg ggcgccactg cttttcatct tcctgtcaat
     1801 cggcatgctc gccggtgagg atggcccact cgggatcgtc ttcaatgact accgcctgac
     1861 ctatacgatt ggctccatcg cgctggcggt catccttttt gatggcggtc tcagaaccag
     1921 gctcgtggtg ttccagagaa gcctgctgcc tgcgggcatt ctcgcaacgt tgggagttgt
     1981 ggccacagcc ggggccatag gcttggttgc gagttggctc cttggcataa cccttctgga
     2041 gggttttttg gtgggggcaa tcgtcagctc aactgatgcc gccgcggtgt tctacctgct
     2101 gcgcacaggt ggcctgcagc tcaagcggcg catcggtgcg accttggaaa tcgaatccgg
     2161 caccaacgac ccggcaagcg ccttcctgac gatactgctt gtccagctta ttctggccgg
     2221 gaatggcgat ggctatgcgg gaatcctcca gtttctgttg acccagatcc tgcttggaac
     2281 cgcaatcggc attggcggtg gctttgttct tgtttgggca ctgggcaacc tgaggctccc
     2341 cccagggttg cgcccgctgt ttgtcgtggg aggtgccatc gccatttttg ctctcacgtc
     2401 cctcctcagc ggcagcggct tcctggccgc ctacctcgcc ggactggttc tgggaaatcg
     2461 cgaagcgcgc gggtttccgg caatcctcag tttccacgac gcagcgacct ggctggcgca
     2521 aattgccatg ttcctcattc tcgggctttt ggtgactcca agtaaactgt tggactactt
     2581 cctgcccgcc ctggcgatag cggccttcct cattgtggtc gcccgcccgg ccgctgtctt
     2641 tctc
//
LOCUS       DAORVN010000270         2634 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_37139_length_2634_cov_2.981388, whole genome shotgun sequence.
ACCESSION   DAORVN010000270 DAORVN010000000
VERSION     DAORVN010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2634)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2634)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2634
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37139_length_2634_cov_2.981388"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1131
                     /gene="phnA"
                     /locus_tag="PKV23_06255"
     CDS             <1..1131
                     /gene="phnA"
                     /locus_tag="PKV23_06255"
                     /EC_number="3.11.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020180335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047400 - phosphonoacetate hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019636 - phosphonoacetate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetate hydrolase"
                     /protein_id="HQX85073.1"
                     /translation="EGLMPNLKKMIARGENTRGLCVIPSFTNPNNLSIVTGAPPSVHG
                     ICGNYLIDPVTGLETMMNDPKWLRAPTIFERFQKAGAKIVMVTAKDKLRLLLGKGLVF
                     DGTAVAFSSEHADKATQKDNGIEGVCDMVGMAVPEVYSAELSEFVFAAGVKLMASMRP
                     DLMYLSTTDYVQHKYAPGSKGANSFYKMMDGYLGQLDAQGAIVVIVADHGMKAKHLPS
                     GEPDVIYLQDAMDKSFGAGATKVILPITDPYVQHHGALGSFATVYVKGISVADASAFI
                     AKQPGIDVVLDRAQGCARFELPEDRMGDIIVVSGGSTGSKVIGTSRARHDLSGLNEPL
                     RSHGGLTEQTIPVIVNRKTQNLPKNLRNFDAFFIGCNCIIEA"
     gene            1133..2578
                     /gene="phnY"
                     /locus_tag="PKV23_06260"
     CDS             1133..2578
                     /gene="phnY"
                     /locus_tag="PKV23_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010975823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde dehydrogenase"
                     /protein_id="HQX85074.1"
                     /translation="MTRTVITESMRIGGKKVSADGVVPVHYPFTNEIIGTVPAGRAEH
                     AARAFEIAAGFKAKLTRYERQQILFKTADLIRQRRDSLAQVLTLELGISMKDSVYECG
                     RAYDVFSLAAQLCILDDGQIFSCDLTPQGKSRKIFTKRDPVGVISAITPFNHPLNMVS
                     HKIAPAIATNNCVVCKPTELTPLTALALADILYDAGLPPEMFQVVTGWPDDIGDEMVT
                     NPHIAVITFTGGVRVGKLIAAKAGYKRAALELGGNDPLIVLNDLSDSDLDKAAELAVA
                     GSTKNSGQRCTAVKRILVQNRIADKFVALVLEKAKKIKFGDPMDPATDLGCVVHEEAA
                     KVFEARVHKAAEQGAKILYNPGRQGAVLPPITVDFVPHTSELVFEETFGPIIPIVRVP
                     DDDAEVIRISNSTPFGLSSGVCTNDLGRITRYIEGLNVGTVNIWEVPGYRIEMSPFGG
                     IKDSGNGVKEGVMEAMKFFTNVKTWSLPWPS"
BASE COUNT          563 a          754 c          774 g          543 t
ORIGIN      
        1 gagggcctga tgccgaatct caagaaaatg attgccaggg gcgaaaacac ccgcggactt
       61 tgcgtcattc caagcttcac caatcccaat aatctttcga tcgtgaccgg ggcgccgcct
      121 tcggttcacg gaatctgtgg aaactatctc atcgatcccg tgacagggct cgagacgatg
      181 atgaatgacc cgaaatggct gcgggctccc accatcttcg aaagattcca gaaggccgga
      241 gccaagatcg tcatggtcac ggccaaggac aagttgcgcc tgctccttgg caagggcctg
      301 gtttttgacg gaaccgccgt tgccttttct tcagaacatg ccgacaaggc gacccagaag
      361 gacaatggca ttgaaggcgt ttgcgacatg gtcggaatgg ccgtgccgga ggtctattcg
      421 gcggagcttt cggaattcgt gtttgcggcg ggtgtgaagc tcatggcttc gatgaggccg
      481 gacctcatgt acctctcgac gacggactat gtgcagcaca agtatgcgcc gggctcgaag
      541 ggcgcaaaca gcttctacaa gatgatggat ggctatctcg ggcagcttga cgcgcaagga
      601 gctatcgtcg tgatcgtggc cgatcatggc atgaaggcca agcatctgcc cagcggcgaa
      661 cccgatgtga tttacctgca ggacgccatg gacaagagct ttggcgcggg ggctacaaaa
      721 gtcatcctgc ccattaccga tccatacgtg cagcaccacg gggcgctggg ctcatttgca
      781 acggtctatg taaagggaat ttcagttgca gacgcttcag cgttcattgc caagcaaccc
      841 ggcattgatg tggtccttga ccgggcccag ggctgtgccc gcttcgagct gccggaagac
      901 cggatgggcg acatcatcgt tgtttcaggc gggagcacgg gatcgaaagt gatcgggacc
      961 agccgtgcgc ggcacgacct ttcaggactg aatgagccgc tgcgttccca tggcggcctc
     1021 acggagcaaa ccattccggt catcgtgaac cgcaagaccc agaacctgcc gaagaacttg
     1081 cgcaatttcg acgccttttt catcggctgc aactgtatca tcgaggcctg acatgacccg
     1141 caccgtgatc accgaaagca tgcgtatcgg cggcaagaaa gtttctgccg atggcgtcgt
     1201 gccggttcat tatcccttca ccaatgagat catcggcacg gtcccggctg gccgggccga
     1261 acatgcggcg cgggcctttg aaattgccgc gggcttcaag gcaaagctca cgcgctatga
     1321 acgccagcag attctgttca agacggcgga tctcattcgc cagcgccgcg attcccttgc
     1381 tcaagtgctc acgcttgagc tcggcatctc aatgaaggat tcggtctacg aatgcggccg
     1441 cgcctatgat gtgttctcgc tggcggccca gctttgcatt ctcgatgacg ggcagatctt
     1501 ttcctgtgac ctcacgccgc aaggcaaatc gcgcaagata ttcaccaagc gcgatcccgt
     1561 cggggtgatc tcggccatca cgccgttcaa ccatccgctc aacatggtat cgcacaagat
     1621 cgcgccagcc attgccacga ataactgcgt ggtctgcaag cctacggagc tcacgccgct
     1681 cacggcgctg gcacttgccg atattctcta tgacgcgggc ctgccgccgg aaatgttcca
     1741 ggtggtcacc ggctggcccg atgacatcgg cgatgaaatg gtgaccaatc cccatatcgc
     1801 ggtcattacc tttaccggcg gggtgcgggt aggaaaactc attgcagcaa aggcaggcta
     1861 caagcgggcg gcccttgaac tgggcggcaa tgatccgctg attgtcctga acgacctgag
     1921 cgacagcgat ctcgacaagg cggcggagct tgccgttgcc gggtccacca agaattcggg
     1981 ccagcgctgc acggcagtca agcgcatcct ggttcagaac cggattgctg acaagttcgt
     2041 ggcactggtc ctggagaaag ccaagaaaat caagtttggc gatcccatgg accccgccac
     2101 ggatctgggc tgcgtggtgc atgaggaagc ggccaaggtc tttgaggccc gggttcacaa
     2161 ggccgccgaa caaggggcta aaatcctcta caacccaggg cgccagggag cggtgcttcc
     2221 gcccatcacg gtggacttcg tgccgcacac gtccgaactg gttttcgagg agaccttcgg
     2281 tcccatcatc cccatcgtcc gggttccgga cgatgatgcg gaggtgatcc gcatttcaaa
     2341 ttccacgccc ttcgggcttt cctcgggcgt ttgcaccaat gacctcggcc ggattacccg
     2401 ttacatcgag ggcctgaacg tgggtacggt taatatctgg gaagtgccgg gttaccgtat
     2461 tgagatgtcg ccctttggcg ggatcaaaga ctcaggtaat ggtgtgaagg aaggggttat
     2521 ggaagccatg aagttcttca ccaacgtgaa gacatggtct ttgccatggc caagttgaac
     2581 ggcattgaat ccaggcacgc tcgttatcat caatctgctc ggtggcgtcg ccct
//
LOCUS       DAORVN010000271         2619 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_37550_length_2619_cov_2.800702, whole genome shotgun sequence.
ACCESSION   DAORVN010000271 DAORVN010000000
VERSION     DAORVN010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2619)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2619)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2619
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37550_length_2619_cov_2.800702"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="PKV23_06265"
     CDS             <1..194
                     /locus_tag="PKV23_06265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85075.1"
                     /translation="IKRADFASSSVDVRQRISEINAMWPGLPEEVLTGKLLPRHTRRQ
                     QQPVTIANVAASDEVVGLI"
     gene            337..867
                     /locus_tag="PKV23_06270"
     CDS             337..867
                     /locus_tag="PKV23_06270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85076.1"
                     /translation="MAKDFLRFCFLIPHGIAKGFTDREGWAVTAERFSERRLLIPVMP
                     SGELADFVRVASNTSTRFSAELSSIEERMAELLEKTREVPGWNVCLYIQRRDGRSASL
                     RWRSADGGIVPDTDLLPSLKDYPLALQEWYRFVHANVLWLNASERVCRRLAAEYSALA
                     DSVSGVMAGRGAGLPA"
     gene            945..1346
                     /locus_tag="PKV23_06275"
     CDS             945..1346
                     /locus_tag="PKV23_06275"
                     /inference="COORDINATES: protein motif:HMM:NF012651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HQX85077.1"
                     /translation="MAIEATLNGNLLTAPVTKMAPTKDGQKPVVELRVMAAYYREESG
                     DGGQTRYIQDDKKTFPVQVSIWNERLGEQVMKLCKTGASVTCTGTLFASPYADGNGQP
                     QAGLNLSANSVKLGLSRVEDVIYRKNAAADS"
     gene            1440..2192
                     /locus_tag="PKV23_06280"
     CDS             1440..2192
                     /locus_tag="PKV23_06280"
                     /inference="COORDINATES: protein motif:HMM:NF017619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement resistance protein TraT"
                     /protein_id="HQX85078.1"
                     /translation="MSLLRSSVVAFGLIGVIPMLAGCAATQVAISKRDLDVQTKMSAT
                     VFLDPVKENDRTVLVQFRNTSDKQTLDLYADLATAVANKGYRITTNPDEAHYLLQANV
                     LYVGKVSPTAAEVALKGGYGGALSAGMGMAALAYAAGHRNDQGLVGAGFLGAVGNTIA
                     NAMVKDVYFSIVTDIQIKERAGNGTRVNIDARHTLAQGTSGASQSSFNETSDWKTYQT
                     RVISTANKVNLEFDEAVPELRTGLVRSLAGIF"
     gene            2194..2577
                     /locus_tag="PKV23_06285"
     CDS             2194..2577
                     /locus_tag="PKV23_06285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85079.1"
                     /translation="MNNWEWQMSQHYFDTMHEGSRVLVLMGWDRPLAHYFMVIERNPP
                     GYIEDDDEDGASDDGCILYSNLDDHNAFRQGLADFKIKLAEFGIQVPGSMFEQVERDQ
                     ALNIGNRQVWYGTDGSFSEDRKACA"
BASE COUNT          603 a          600 c          805 g          611 t
ORIGIN      
        1 tcatcaagcg cgccgacttt gcatcaagca gcgtggatgt gcgccagagg atttcagaga
       61 tcaatgccat gtggccggga cttccggagg aagtgctgac aggcaagcta ctgccacggc
      121 acacaaggcg gcaacaacag ccggtgacga tcgcaaatgt ggcggcgagt gatgaggtag
      181 ttgggctgat ttgaggtagt gaaggtgttt agggtctaaa cactttcggg cgattggcag
      241 ggtaaggcaa cgggtgttta gaccctaaac acttgaagag gattggcggt gtgtgacaga
      301 ggtgtttaga ccctaaacac cttgaggcgg aaaaatgtgg cgaaagattt tctgagattt
      361 tgttttctga taccgcacgg tatcgcaaag ggctttacgg atagggaggg ttgggctgtg
      421 acggcagaaa gattttcgga gaggcggttg ttgattcctg taatgccttc gggcgaactg
      481 gctgacttcg ttcgcgtggc ttcaaacact tcgacgcggt tttctgctga gttgagttcg
      541 atagaagagc gtatggctga actgcttgag aagacccgcg aggttccggg atggaacgtc
      601 tgtctgtata tccagcgtcg agatgggcgc agtgcctcgt tgagatggcg atctgctgac
      661 ggcggcatcg tgcctgatac agatttgttg ccctccttga aagactatcc gctggcgctt
      721 caggagtggt atcgcttcgt ccatgccaat gtgctttggc taaatgcgtc ggagcgagta
      781 tgccgtcgat tagctgccga atattcggca ttggcggatt ctgtttctgg cgtgatggcc
      841 gggcgcggag ctggcctgcc agcgtagcgc catcctggca gtcgtcagga tatggcgcag
      901 acatcgtttc attattcttg gcttgattta taacggaggt tcaaatggca attgaagcaa
      961 ccctgaatgg caatcttctg acagcgcctg tcaccaaaat ggcgccgacc aaggacggtc
     1021 aaaaacccgt cgttgagctt cgcgtcatgg cggcgtacta ccgcgaggaa tctggcgatg
     1081 gcgggcaaac ccggtatatc caggacgaca agaaaacctt ccccgtccag gtgtccatct
     1141 ggaacgagcg gcttggcgag caagtcatga aactttgcaa gaccggcgcc agtgtgactt
     1201 gcacaggcac gctgtttgcc agcccctatg ccgacggcaa tggacagccc caagctggac
     1261 tgaatctatc ggccaattct gtgaagctcg gcttgtcccg cgtcgaggac gtgatttatc
     1321 ggaaaaatgc tgctgcagat tcctagaatt ttcatgacat aagtgacacg gagatgagca
     1381 gtttttgagg attccggtac aggtgtcagg tctgcgtcgt atttattgct gaggtaatca
     1441 tgagtttatt gcgcagttca gtagttgctt ttggattaat tggagtaata ccgatgctcg
     1501 ccggttgcgc agcgacgcaa gtggcaatca gcaaacgcga tctggatgtc cagaccaaga
     1561 tgagcgccac agtttttctg gatcctgtga aagaaaatga ccgaacggtt ctggttcaat
     1621 tccgcaatac atcggataag cagactctcg atctgtatgc ggatctggca actgcggtgg
     1681 ccaacaaggg ctatcggatt acgacgaatc cggatgaggc gcattacctg ctgcaggcga
     1741 atgtgctgta tgtcggcaag gttagcccga ctgcggcgga ggttgcactc aagggcggct
     1801 atggcggtgc tttaagtgca ggcatgggaa tggctgcgct tgcttatgca gcaggccaca
     1861 ggaacgacca ggggctggtc ggcgcaggct tcctgggtgc ggtgggaaac accatcgcta
     1921 atgcgatggt caaggatgtg tatttcagca tcgtcacgga tatccagatc aaggaacgcg
     1981 caggtaacgg tacgcgagtc aatatcgacg cgcgacatac cctggctcaa ggaacctcgg
     2041 gggccagtca atcaagcttc aacgaaacga gcgactggaa gacttatcag acgcgagtaa
     2101 tttccacagc caacaaggtc aatctggagt tcgacgaggc ggtgccggaa ttgcgtactg
     2161 gcctggtgcg cagcctggca gggatattct gatatgaata actgggaatg gcagatgagt
     2221 cagcactatt tcgacacgat gcacgaagga tcccgtgtcc tggttctcat ggggtgggat
     2281 cggcctttgg cccattactt catggtcatt gagcgcaacc ctcccggcta tatagaggac
     2341 gacgacgagg atggtgccag tgatgatgga tgcattctct actctaacct tgatgatcac
     2401 aatgcgttca ggcagggtct tgccgacttc aagataaagc tcgcggagtt tggcattcag
     2461 gttccggggt cgatgttcga acaggttgag cgcgatcaag ccctgaacat tgggaatcgc
     2521 caagtctggt atggcaccga tggttcattt agcgaggatc ggaaagcttg tgcgtgaggc
     2581 agatggtcac tgggtatgtc atggcgtgag cggcaaatt
//
LOCUS       DAORVN010000272         2611 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_37749_length_2611_cov_2.853678, whole genome shotgun sequence.
ACCESSION   DAORVN010000272 DAORVN010000000
VERSION     DAORVN010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2611
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37749_length_2611_cov_2.853678"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..582)
                     /locus_tag="PKV23_06290"
     CDS             complement(<1..582)
                     /locus_tag="PKV23_06290"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020095544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HQX85080.1"
                     /translation="MNFLLRMSSFVLICVLAGSSAVWAVTRQPVAAENGMVVTSQHLA
                     SKVGVEILKQGGNAVDAAVAVGYALAVANPCCGNIGGGGFATLHLADGRGIFINFREK
                     APLAATQTMYLDAAGEVVPDLSLKGYLAVGVPGSVLGLDTLLQKFGTMSRAAVMAPAI
                     RLAEEGFVLSQGDADILAISTKHFVAQPNVAAIF"
     gene            complement(681..2084)
                     /locus_tag="PKV23_06295"
     CDS             complement(681..2084)
                     /locus_tag="PKV23_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018183168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HQX85081.1"
                     /translation="MKVLAIDQGTTATKSFTLDGEGRFESVAAFEHRQIFPNAGWVEH
                     DPEELLRNVSACLEVAGKVDAIGMDNQGETVIAWDAGTGKPIYNAIVWQDSRTQDATE
                     KLKADGAEELTLARAGLPLDPYFSAGKLRWLLDHADGARSLLKQDRLRLGTSDAFFLD
                     RLTGTFATDVTTASRTSLMSLDTCTWDPELCKLFGVPIECLPEIRSTAGDFGQVGKTP
                     VTASLVDQQAALFGHGCPTKITFGTGAFALTVTGEGRVDGSKFGILPTLAWKIKSEPA
                     QRAIEGGVYNAGSAVNWARGLGLFREFSGIENFEMPSALSRNLVFVPALSGLACPHWD
                     RGAAGLWIGMGLETTAKDLIQALLEGIALRAAEVIDAMAELTPLGNMISIDGGLTKNS
                     YFNRFLANALQRTLIVQESPELTGLGTARMAMRGAGLKSLPPLPEPRASIKPDAPLGR
                     ELKQRFSEAVKRAKNWK"
     gene            complement(2081..>2611)
                     /locus_tag="PKV23_06300"
     CDS             complement(2081..>2611)
                     /locus_tag="PKV23_06300"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HQX85082.1"
                     /translation="TPRSARLVGGSRPQGVMNTGALQQHVYLHHNKPFERPVIIGSEL
                     VSFSALLTCRHLGIRPVAMLEENPRISAQRPGDLIARFVLGVPVRTSTKLVAIEGRKN
                     VEAVEVEHRGKRERIACDGVIFTGLFRPETALFPAGLPSEKFSISGNAVGPLKTSGEC
                     WLQGVAAAEGIARKLT"
BASE COUNT          527 a          767 c          816 g          501 t
ORIGIN      
        1 aaatatggcc gcgacattgg gctgagcaac gaaatgcttg gtcgatatcg caagaatatc
       61 ggcgtcaccc tggctgagaa cgaagccctc ctcggcaagc ctgatcgctg gcgccatgac
      121 agcggcgcgc gacatcgtgc cgaatttctg cagcagggta tcgagcccga gcaccgaccc
      181 cggtacgcca actgccagat aacctttgag gctgagatcg ggcaccacct cgcctgcagc
      241 atcgagatac atggtttgcg tggcagcaag cggcgccttt tcgcggaagt tgataaaaat
      301 gccgcggcca tcggcgagat gcagcgtggc aaatccgccg ccgccgatat tgccgcaaca
      361 gggattggcg actgcgaggg catagcccac tgccaccgcg gcatcgaccg cgttaccacc
      421 ctgcttcaaa atctccacgc ccaccttcga ggcgaggtgc tgcgatgtaa ccaccatgcc
      481 gttttcagcc gctacaggtt ggcgggtcac cgcccagaca gcggaactgc cggcaagcac
      541 acagatcaga acgaacgaag acatgcgaag aaggaagttc atgctaatcg ccttatgcgg
      601 ttaagtatgt aagcacccag ttgacgtcac ttccggagca agatcaagcc atgatcagaa
      661 ctcgaagctg tgggtttcgt tcatttccag ttcttcgccc gcttcacggc ttccgaaaat
      721 cgctgcttca attcccggcc cagcggcgca tcgggcttga tcgaagcgcg cggttccggg
      781 agcggcggca atgatttcag gccagcgccg cgcattgcca tgcgggccgt gccgaggccg
      841 gtgagctccg gggattcctg aacgattaga gtcctttgca gggcattggc gagaaagcgg
      901 ttgaaatagg aattcttcgt gagcccgcca tcgattgaaa tcatgtttcc gagtggcgtg
      961 agttccgcca tcgcatcgat cacttccgcg gcgcgcaggg caatgccttc gagcagtgcc
     1021 tgaatcagat cctttgccgt ggtttccagt cccatgccga tccacaatcc ggcggcgcca
     1081 cgatcccaat gcgggcacgc gagacctgaa agcgccggca caaaaacaag attgcgcgac
     1141 agggctgatg gcatttcaaa attttcgata ccggaaaatt ccctgaagag gccaaggccc
     1201 cgggcccagt tcaccgccga gccggcgttg taaacgccgc cctcgatggc gcgctgcgcc
     1261 ggttcacttt ttattttcca ggcaagggtt ggaaggatgc cgaatttcga accatcgacg
     1321 cgcccctcac cggtgaccgt cagggcgaag gcgccggtgc cgaaagtgat cttggtgggg
     1381 catccgtggc cgaagagcgc ggcctgctga tcgacaaggc tggcggtgac cggcgttttt
     1441 cccacctggc cgaagtcgcc cgcggttgac ctgatttcgg gcaggcattc gatgggcacg
     1501 ccgaagagtt tgcaaagttc gggatcccag gtgcaggtgt cgagcgacat gaggctggtg
     1561 cgcgaggcgg ttgtcacatc ggtggcaaag gttccggtga ggcgatcaag gaagaacgca
     1621 tcgctggtgc cgaggcgcaa gcggtcctgc ttgagaaggc tgcgggcccc gtccgcatga
     1681 tcaaggagcc agcggagctt gccggcggaa aaatagggat caagcggcag gccggcgcgg
     1741 gccagggtga gttcctcggc tccatcggcc ttcagctttt ccgtggcatc ctgggtgcgg
     1801 ctgtcctgcc agacgatggc gttgtagatg ggcttgccgg tgcctgcgtc ccaggcgatc
     1861 accgtctcgc cctgattgtc catgccgatg gcatcgactt tgcctgcaac ctcaaggcag
     1921 gcgctgacat tgcgcagcag ttcttccggg tcatgctcaa cccagccggc attggggaaa
     1981 atctgccggt gctcgaaggc ggcaacggat tcaaagcggc cctccccatc aagggtgaag
     2041 gatttcgtgg cggttgttcc ctggtcaatg gccagaacct tcatgtcaat ttccttgcga
     2101 taccctcggc cgcagccaca ccctgcagcc agcattcgcc ggaggttttg agcggaccca
     2161 cagcatttcc ggagattgag aatttttccg atggcaggcc ggcggggaac aatgcggttt
     2221 caggccggaa gagcccggtg aaaatgaccc catcacaggc gatgcgctcg cgtttgccgc
     2281 ggtgctcaac ttcaaccgct tcaacgttct tgcggccttc gatggcaacg agtttagtgg
     2341 aggtgcggac cggcacgcca agaacaaatc tggcgatgag atcacccggc ctttgagccg
     2401 agatgcgcgg attctcctcc agcatggcta cgggcctgat gccgaggtgg cggcaggtga
     2461 gcagcgccga gaaggacacc agttcgctgc cgatgatcac cgggcgttca aagggtttgt
     2521 tgtgatggag atagacgtgc tgctgcaggg cgccggtgtt catcacgccc tgcgggcggc
     2581 ttccgcccac taggcgggcc gaacgcgggg t
//
LOCUS       DAORVN010000273         2610 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_37776_length_2610_cov_3.239922, whole genome shotgun sequence.
ACCESSION   DAORVN010000273 DAORVN010000000
VERSION     DAORVN010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2610)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2610)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2610
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37776_length_2610_cov_3.239922"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /locus_tag="PKV23_06305"
     CDS             <1..396
                     /locus_tag="PKV23_06305"
                     /inference="COORDINATES: protein motif:HMM:NF027645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54 modulation/S30EA ribosomal C-terminal
                     domain-containing protein"
                     /protein_id="HQX85083.1"
                     /translation="GLVLQSHGSAAEAFQSFDAAAQHLEKQLKRYKQRLKDHHKDRRE
                     PVRQSEAASYVIQAGNEEGEEPASLNPVVIAESSTSVPELSVGEAVMQLEISSVPFVL
                     FRNSKGGQTNVVYRRSDGNIGWVDPRPAA"
     gene            411..881
                     /gene="ptsN"
                     /locus_tag="PKV23_06310"
     CDS             411..881
                     /gene="ptsN"
                     /locus_tag="PKV23_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008548629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HQX85084.1"
                     /translation="MTDLADIIDARAVLPSVKAQGKKQLLQDLAQAMAKFVAVDHRII
                     FETLLTREKLGSTGIGQGIAIPHGKLASINRVYGLFARLSTPIAYDSLDGQPVDLVFV
                     LLAPDHAGADHLKALARISRLLRENDTIAKLRGTDTAEGLYSILTEPSAAASAA"
     gene            complement(927..1190)
                     /locus_tag="PKV23_06315"
     CDS             complement(927..1190)
                     /locus_tag="PKV23_06315"
                     /inference="COORDINATES: protein motif:HMM:NF018343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1150 family protein"
                     /protein_id="HQX85085.1"
                     /translation="MNTSNSKEGFELSPEELAQLGNGALSYIREIEGRDVIRLVGPQA
                     RVAPDAKLFCLFNANGSPISISGTREAAADSAVEHELVAISLH"
     gene            complement(1201..1617)
                     /locus_tag="PKV23_06320"
     CDS             complement(1201..1617)
                     /locus_tag="PKV23_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002965411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20 family protein"
                     /protein_id="HQX85086.1"
                     /translation="MSKITMFSSPLLLGFDDLERLLERAVKTSGDGYPPYNIERLADG
                     GNEVLRITIAVAGFQRQDLEITIEDNQLNIRGRTKDEEPRDYLHRGIAGRQFARAFVL
                     ADGMEVIDATLVNGLLIINLRRPEPSSVIRRIEIKE"
     gene            1725..2504
                     /locus_tag="PKV23_06325"
     CDS             1725..2504
                     /locus_tag="PKV23_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013891896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQX85087.1"
                     /translation="MAGILDRVALVTGAGSAEGIGFATARALAAAGARVAITSTTKRI
                     FERLETLGAGHAAFTADLTIVEDVSELINAVQAKFGRIDIVVNNAGMVQQGTNFKSSR
                     IEKITDEEWLRHLTLNVTTAFNVTRAVLPLMQKQEYGRIVNVASVTGPLVTNPKAAGY
                     SSAKAAMTGLTRVTAVENASRNITCNAVLPGWIETASSSKEEILAGKATPSKRPGRPD
                     EIAAACLFLASEEASYVNGAMLVVDGANSIVEFKGAGDDYY"
BASE COUNT          548 a          810 c          750 g          502 t
ORIGIN      
        1 ggcctcgtcc tgcaatcgca cggctccgcg gcagaggcct tccagagttt tgatgcggcc
       61 gcccagcatc ttgaaaagca gttgaagcgc tacaagcagc gcctgaagga tcatcacaag
      121 gaccgccgcg aaccggtgcg ccagtccgag gccgcaagct atgtcattca ggcgggcaat
      181 gaagaaggcg aggagccagc aagcctcaac ccggtggtca tcgccgaatc atccaccagc
      241 gttccggagc tttccgtcgg agaagccgtc atgcagcttg aaatttccag tgtccccttc
      301 gtcctgttca ggaacagcaa gggtggccag accaacgtgg tttaccggcg cagcgatggc
      361 aacatcggct gggtcgatcc caggcctgca gcgtaagtgg aagtgcaagc atgacagatc
      421 tcgcagatat tatcgatgcc cgcgccgtat tgcccagcgt gaaggcgcag ggcaagaaac
      481 agctccttca ggatctggcg caagccatgg caaagtttgt ggcggtcgat catcgcatca
      541 tatttgaaac cctgctgacc cgcgaaaaac tgggctccac cggcatcggc cagggcattg
      601 ccattcccca cggcaagctg gcctccatca accgcgtcta tgggctgttt gcccgcctga
      661 gcacgccgat tgcctatgat tcgctggatg gccagccggt ggatctggtc ttcgtcctgc
      721 tggcccccga ccacgccggc gccgaccacc tcaaggcgct cgcgcggatt tcaaggctgt
      781 tgcgtgagaa cgacaccatc gcaaaactgc gcggcaccga caccgccgaa ggcctctact
      841 caattctcac cgagccctcc gccgctgcct ctgccgccta atttcttcct cgcccccatg
      901 caaatggggg agaggaaagt tgaattttaa tgcaagctga tcgccaccag ctcatgctcc
      961 acggcgctgt cggctgcggc ttcgcgggtt cccgaaatcg agatcggcga gccattggcg
     1021 ttgaacaggc agaaaagctt cgcatcgggt gccacccgcg cctgcgggcc gaccaggcgg
     1081 atcacgtcac ggccttcgat ttcgcggata taggacagcg caccgttgcc gagctgggcc
     1141 agctcttccg ggctcagctc gaagccttcc ttgctgttgc tggtgttcat gctgctactc
     1201 ctattcctta atctcaatac gccggatgac actgcttggt tccggtctcc ttaagttaat
     1261 aattaaaagt ccgtttacaa gggtggcgtc tatcacctcc atgccgtccg ccagcacgaa
     1321 tgcccgggcg aactgccgcc cggcaatgcc gcggtgcagg taatcgcggg gttcttcgtc
     1381 ttttgtccgc ccccggatgt tcaattggtt gtcctcgatg gtgatctcca ggtcctggcg
     1441 ctggaacccg gcaaccgcga tggtaatgcg cagaacctcg ttgccgccat cggccagtcg
     1501 ctcgatattg taaggcggat aaccatcgcc ggaggttttg acggcccgct cgagcaggcg
     1561 ttccagatcg tcaaacccca gcaaaagggg cgaggaaaac atggtaatct tgctcatcgg
     1621 acttttgccc tcgctaaagc ggcttcaagg atgctacggc ccatctggca ccgcagcctg
     1681 attgctaata tgagagtgcc ggaagctgat ttcaaggaaa tgtcatggca gggattttgg
     1741 atcgtgtggc gctggtaaca ggtgctggaa gtgctgaagg tatcggtttt gcaacagcca
     1801 gggcgcttgc cgcggcaggg gcgcgggtgg ccattacctc gaccacaaaa cgcatcttcg
     1861 agcgcctcga aaccctcggc gctggccatg ccgcattcac cgccgacctg accatcgttg
     1921 aagatgtcag tgaactgatc aacgcagtgc aggcaaaatt cggccgcatc gacattgtcg
     1981 tgaacaatgc cggcatggtc cagcagggca ctaacttcaa aagttcaagg atcgagaaaa
     2041 tcactgacga ggaatggctg cgccatttga cgctcaacgt caccaccgcc ttcaatgtca
     2101 cccgcgccgt gctgcccctc atgcagaagc aggaatatgg ccgcatcgtc aacgtggcct
     2161 ccgtgaccgg cccgctcgtc accaatccga aggccgccgg ctattcctcc gccaaggccg
     2221 ccatgaccgg cctgacgcgc gtgacggcag ttgaaaacgc cagccgcaac atcacctgca
     2281 acgccgtgct gccgggctgg atcgagaccg cctcgtcaag caaggaagaa attctggcag
     2341 ggaaagccac gccgtcgaaa aggccgggcc gccctgatga aatagccgcc gcctgcctgt
     2401 tcctcgccag tgaggaagca agctacgtca acggcgccat gctggtggtc gatggcgcaa
     2461 actcaatcgt cgagttcaag ggagcaggcg atgattatta ctgacgattg gaccggcaaa
     2521 actgtcctca tcgtcggcgg cagcggcggc atgggccgcg ccaccgccca ggaatttgcc
     2581 ggagccggcg cgaacatcgc catcgctgat
//
LOCUS       DAORVN010000274         2610 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_37790_length_2610_cov_2.671233, whole genome shotgun sequence.
ACCESSION   DAORVN010000274 DAORVN010000000
VERSION     DAORVN010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2610)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2610)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2610
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37790_length_2610_cov_2.671233"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..111)
                     /locus_tag="PKV23_06330"
     CDS             complement(<1..111)
                     /locus_tag="PKV23_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006467003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HQX85088.1"
                     /translation="MNIILLGPPGAGKGTQAKLLEVGRGMKQLSTGDMLRA"
     gene            complement(108..1439)
                     /gene="secY"
                     /locus_tag="PKV23_06335"
     CDS             complement(108..1439)
                     /gene="secY"
                     /locus_tag="PKV23_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HQX85089.1"
                     /translation="MASAAEQLAANLNFGALAKSEDLKSRIWFTLGALIVYRLGTYIP
                     IPGIDTVELARFAAQNSSGILGWVNGLAGGALGRMAIFALNIMPYISASIIMQLMTTV
                     SPHLEALKKEGESGRKKMNQYTRYGTVILAALQAYGIAVGLEGQGNLVADPGLFFRAS
                     TVITLTGGTVFLMWLGEQITSRGIGNGISLIIFAGIVAGLPGGIAAMLELGRTGQLST
                     FVILGIMIMALGVVAFIVLFERAQRRLLIQYPKRQVGQKVFQGDSSHLPLKLNTAGVI
                     PPIFASSLLLLPITVANFSAGQGPDWLISITALLGHGQPLFLALYVMLIIFFAFFYTS
                     IMFNPKETAENLKKYGGFIPGIRPGERTAEYIDYVLTRVTVVGALYLSLVCLLPDLLI
                     GYTGVPFYFGGTSLLIVVSVTMDTVAQLQGHLYAQQYEGLIKKSKLRGNRK"
     gene            complement(1503..1991)
                     /gene="rplO"
                     /locus_tag="PKV23_06340"
     CDS             complement(1503..1991)
                     /gene="rplO"
                     /locus_tag="PKV23_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014494508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HQX85090.1"
                     /translation="MKLNEIADNAGARKIRKRVGRGLGSGLGKTAGRGGKGQTARKGG
                     AKAGFEGGQMPIYRRLPKRGFNKPNQVSFNEVNLQTVQAAVDGKKLDTKAAVTAATLI
                     AAGVISRPLGGVRLLAKGEIKSKLAFEVFYATKGAIAAVEKAGGSVKVLRPVEVPSEH
                     KK"
     gene            complement(2009..2203)
                     /gene="rpmD"
                     /locus_tag="PKV23_06345"
     CDS             complement(2009..2203)
                     /gene="rpmD"
                     /locus_tag="PKV23_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008837731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HQX85091.1"
                     /translation="MAKAPAAKTVTVTQYASPQRRPAEQRATLVGLGLNKINRRSTLN
                     DTPAVRGMIAKVAHLVRVVE"
     gene            complement(2221..>2610)
                     /locus_tag="PKV23_06350"
     CDS             complement(2221..>2610)
                     /locus_tag="PKV23_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018699148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HQX85092.1"
                     /translation="FGHGKAREVPEAIRKATEAAKRVLIRVPLREGRTLHHDIDGRWG
                     AGKVTLRAAPPGTGIIAGGPMRAVFESVGVADVVAKSKGSSNPYNMVRATFDALKKQT
                     SPRSVAARRGKKVSDVLRRRDPDAAVE"
BASE COUNT          521 a          805 c          763 g          521 t
ORIGIN      
        1 tgcacgcagc atgtcgcccg tggacagttg cttcatgccc cggccaacct caagcagctt
       61 ggcttgcgtg ccctttccgg cacccggagg tccgagcagg atgatgttca tttccggttg
      121 ccgcgcagct ttgatttctt aatcaggcct tcatactgct gggcatagag atggccctgc
      181 agctgcgcca ccgtatccat ggtgacactc acgacgatca gcagggaggt gccgccgaaa
      241 tagaagggca ccccggtata gccgatcagg agatcaggca gcaggcagac cagcgagaga
      301 tagagggcgc cgacaaccgt gacgcgggtc agcacatagt cgatgtattc cgcggtgcgc
      361 tcgcccggac ggatgccagg aatgaagccg ccatacttct tcaggttctc cgccgtttcc
      421 ttgggattga acatgatgga ggtgtagaag aaggcaaaga aaataatcag catcacatag
      481 agcgcaagga acagcggttg gccgtgcccc agaagcgcag taatcgaaat cagccagtcc
      541 ggcccctgcc cggcgctgaa gtttgccacc gtgatcggca gcagcagaag cgacgaggcg
      601 aaaatcggcg ggatcacgcc tgccgtattc agcttgagcg gcaaatgcga actgtcgccc
      661 tggaatacct tctgcccgac ctggcgcttg ggatactgga tgagaagccg ccgttgcgcc
      721 cgctcaaaca ggacgatgaa ggcaaccacg ccaagcgcca tgatcatgat gcccagaatg
      781 acgaaagtgg ataactggcc cgtgcgcccc agctcaagca tggccgcgat gccgcccggc
      841 aagccggcga caatgcccgc gaaaatgatc agggaaattc cgttgccgat gccgcgcgag
      901 gtgatctgtt cgcccagcca catcaggaat accgtaccgc cggtcagcgt gatcaccgtg
      961 ctggcgcgga aaaacaggcc gggatcggca accaggttgc cctgcccttc aagccccacg
     1021 gcgatgccat atgcctgcaa tgccgccagg atcaccgtgc cgtagcgcgt gtactggttc
     1081 attttcttgc ggccggattc gccttccttc ttgagcgctt caagatgcgg cgacaccgtg
     1141 gtcatcagct gcatgatgat ggaggctgaa atatacggca tgatattgag ggcaaaaatt
     1201 gccatgcggc caagcgcgcc gcccgccagg ccgttcaccc agccaaggat gcccgacgag
     1261 ttctgcgctg caaatcgcgc cagttcaaca gtatcaatgc cggggatggg aatgtaggtg
     1321 ccaaggcggt aaacgatcaa tgcacccagg gtgaaccaga tgcggctctt caggtcttcc
     1381 gatttggcca gggccccaaa attaaggttt gccgcaagtt gttcagctgc cgatgccatg
     1441 tcgtatcctt gagctttggt aacaagctcc ctatgtgggg tggcccgtta acccatgcaa
     1501 ccttacttct tgtgttcgga cggcacttca actggcctca aaaccttcac actgccaccg
     1561 gctttttcga ccgcggcaat ggcacccttg gtggcgtaaa acacctcgaa tgcgagtttt
     1621 gacttgatct cacccttggc cagcaggcgc accccgccga gcggacgcga aatcaccccg
     1681 gccgcgatca gcgtagcagc cgtgacggca gcctttgtgt cgagcttctt gccgtcaacc
     1741 gccgcctgga cggtctggag attgacttcg ttaaaggaaa cctggttcgg cttgttgaag
     1801 ccgcgctttg gcagccggcg ataaattggc atctggccgc cttcaaagcc ggcctttgcg
     1861 ccgcccttgc gggccgtctg gcccttgccg ccgcggccgg cagttttgcc aaggcccgaa
     1921 cccaggccgc gtcccacccg cttgcggatc ttgcgggccc cggcgttatc ggcaatttca
     1981 ttgagcttca ttgtcgtatt ccttaagctt attcgaccac gcgaacgaga tgcgcgacct
     2041 tggcaatcat gccgcgaacc gcgggcgtat cgttcagcgt tgaacgccgg ttgatcttgt
     2101 tgaggccaag cccaaccaat gtggcgcgct gttccgccgg ccgccgttgc gggctggcgt
     2161 attgcgtcac tgtgactgtc ttcgctgctg gtgctttggc catgattcta cgtcctcaac
     2221 ttattctact gccgcatccg ggtcacggcg gcgcaaaaca tcgctcacct tcttgccgcg
     2281 gcgcgctgcc accgaacggg ggctcgtctg cttcttcagc gcgtcaaaag tcgcgcgcac
     2341 catgttgtag gggttcgacg aacccttgga cttggccacg acatcggcaa cgccgaccga
     2401 ttcaaatacg gcgcgcatcg ggccgccggc aatgatgccg gtacccggag gggcggcgcg
     2461 cagtgttacc ttgcctgcgc cccaacggcc gtcaatatcg tgatgcaatg tgcggccttc
     2521 gcgcaatgga acgcgaatca gcacgcgctt tgcggcttcg gtcgccttgc ggatagcctc
     2581 aggcacttcg cgcgccttgc catggccgaa
//
LOCUS       DAORVN010000275         2598 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_38100_length_2598_cov_5.278805, whole genome shotgun sequence.
ACCESSION   DAORVN010000275 DAORVN010000000
VERSION     DAORVN010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2598
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38100_length_2598_cov_5.278805"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1217)
                     /gene="xseA"
                     /locus_tag="PKV23_06355"
     CDS             complement(<1..1217)
                     /gene="xseA"
                     /locus_tag="PKV23_06355"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HQX85093.1"
                     /translation="MLVSADILSISELNRRARATLEKKFPLLWVVGEISNLTWAASGH
                     VYFSLKDDAAQARCVMFRSRASSVPWRLENGQQVEAQALVSLYEPRGEFQLTIESLRR
                     AGLGRLFEAFARLKAQLDAEGLFAAGRKRPLPRFPRRIGIISSPGAAALRDVLVALER
                     RARHLQRFLYPTLVQGEAAAAEIAAAINTACRRDECDLLLVVRGGGGIEDLWAFNEEV
                     VARAVVASSLPIISGVGHETDTTIIDLVADLRAATPTAAAELATQGWYEAAAEISALG
                     TALGRAARYRVTRDQQRLDHLSLRLVHPATRLGRARDRLALLESQLDASLARNLRQHR
                     ERLNRTALGLGRTMPRTEKHRGGIQLLAQRLAAAIREQHGKRVTTLDHIAGALAHLNP
                     DATLARGFSVVRDA"
     gene            1492..2244
                     /locus_tag="PKV23_06360"
     CDS             1492..2244
                     /locus_tag="PKV23_06360"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HQX85094.1"
                     /translation="MPRSRSKLQVWAADAGFRHVAQFPDPRFVLPNLPTRAKVRAFLL
                     SGVNHVFSIIEAAGWPIWPLLFASVIAVALIIERTAALRRSKIVPVGLLQSAVAEYRQ
                     SGISDVQLARLETHSPLGRVLAAGMRNAKSSREIMKESIEEAGRGVAHELERFLTTLG
                     TIATIAPLMGLFGTIVGMIEIFGASTATGNNPQALAHGISVALYNTGFGLIIAIPAMI
                     FYRHFRAQVDGFLVDMEQEAVKLVELMQGERK"
     gene            2256..>2598
                     /locus_tag="PKV23_06365"
     CDS             2256..>2598
                     /locus_tag="PKV23_06365"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HQX85095.1"
                     /translation="MNFQRGRQREELEINLIPMIDVLLVILIFLMITTTYSKFSGLEI
                     NLPTADAPQNKEQPDEINVVVTAAGEVMVNKAAVAGRDIPSIVNALRRSAGTGKEPVV
                     IINADAKAAHQS"
BASE COUNT          519 a          757 c          808 g          514 t
ORIGIN      
        1 gcgtcacgaa caacggaaaa gcccctggcc agggttgcat cggggttcag atgggcgagc
       61 gcccccgcaa tatggtccag cgtggtcacg cgcttcccgt gttgttcgcg gattgcggca
      121 gcaagccgct gcgctagcag ttggatgccc ccccggtgtt tttcggtacg tggcatggtt
      181 cggcccagac ccagggccgt gcgattcagc cgttcccgat gctggcgcag gttgcgggct
      241 agggaggcgt caagttgcga ttccagcaat gccaatcgat cccgtgcccg gcccaatcgc
      301 gttgccggat ggacgaggcg cagggagagg tgatccagcc gctgctggtc gcgggtgacg
      361 cgatacctgg cggccctgcc cagcgcagtt ccaagggcgg aaatttcggc ggcggcttcg
      421 taccatcctt gagtggcgag ttcggcggcg gcagtagggg tcgccgcacg caagtcggcg
      481 acgaggtcga ttatggtggt atcggtctcg tgtccgaccc cgctgatgat gggcaggctg
      541 ctggcgacga cggcccgtgc gacgacttcc tcgttgaaag cccacaggtc ttcgatgccg
      601 ccgccgccgc gaaccacaag caacaggtcg cattcatcgc gccgacaggc cgtgttgatt
      661 gccgcggcga tttctgctgc ggcggcctca ccctgcacta acgtagggta aagaaaacgc
      721 tgcaggtgtc gcgcgcggcg ctccagcgcc accagtacat cgcgcagggc ggcggcaccg
      781 ggcgaactga tgatcccgat ccggcgcgga aagcggggta gcgggcgctt acggccggca
      841 gcaaacagcc cctcggcatc cagttgcgcc ttcaatcggg caaaggcctc gaacagccgc
      901 cccagcccgg ctcggcgcag ggattcgatg gtgagctgga attcgccgcg gggttcgtaa
      961 agcgagacca gggcttgggc ttcaacttgc tgcccgtttt ccaggcgcca gggcaccgaa
     1021 ctcgcacgcg aacggaacat gacgcagcgc gcctgggctg catcgtcctt gagtgaaaag
     1081 taaacatggc ccgacgcggc ccaggtcaga ttggaaattt cgccgaccac ccagagcagg
     1141 ggaaacttct tttccagtgt tgcgcgcgcc cgtcgattga gttcgctgat gctgaggatg
     1201 tcggcggaaa cgagcatgtg gggaaatcgt cgaaggcaag gccggggaga gggaaatact
     1261 acagcatcca cacgcgggac cggctttgaa ggccggccgg gcaacattca ggtcttttca
     1321 ggttcacatt taaatagctg aatattattg atatatttct gtacaaaatt tgcgcaggaa
     1381 atgtagaagc caagagcaag gctgtttctg ctctggtgaa ggtcgatgat ccacaatctt
     1441 atccacagat tttgtggata atttgtcgtc actttgatgt ggcgacgcct catgccgcgc
     1501 agccggtcaa agttgcaagt ttgggccgct gatgccggat ttagacatgt cgcgcaattc
     1561 cccgaccccc gttttgtatt gccgaatctt cccactcggg ctaaagttcg cgctttcctg
     1621 ctttccggag tcaaccacgt gttttccatc atcgaggctg ccggctggcc gatctggccg
     1681 ttgctcttcg cttccgtcat cgcagttgcg ctcatcatcg aacgtaccgc ggcactgcgc
     1741 cgcagcaaga tcgttcctgt ggggctgttg caaagcgcgg tcgccgaata tcgtcaaagc
     1801 gggatttcgg atgtgcagct ggcgcgcctc gaaacgcatt ctccgctggg aagggtactt
     1861 gcggcaggca tgcgcaatgc aaagagttct cgcgaaatca tgaaggagtc aatcgaagag
     1921 gcaggacgtg gagtggcgca tgaactggaa cgcttcctga cgacactggg taccatcgcc
     1981 acgattgcgc ctctgatggg tttatttggc accattgtcg gcatgataga aatcttcggc
     2041 gcatcgaccg ccacaggaaa caatccgcag gccctcgcac acggcatttc ggtggcgctc
     2101 tacaacaccg gtttcggcct catcatcgcc attccggcca tgattttcta caggcatttc
     2161 cgggcgcagg tcgacggttt tctggtggat atggagcagg aggcggtgaa actggtcgaa
     2221 ctcatgcagg gtgagcgaaa gtagggggcc cgacgatgaa cttccagcga ggccgacagc
     2281 gcgaagaact cgagatcaac ctgatcccga tgatcgatgt actcctggtg atcctgatct
     2341 ttctcatgat caccaccacc tattccaagt tttccgggct ggagatcaac ctgcctaccg
     2401 cggatgcacc gcagaacaag gagcagcccg acgaaatcaa cgtggtggtg accgctgccg
     2461 gcgaagtcat ggtgaacaag gcggcggtcg ccggtcgtga catcccgtcc atcgtcaacg
     2521 ccttgcgccg ttccgccggc acgggcaagg aaccggtcgt catcatcaat gccgacgcca
     2581 aggccgcgca ccagagcg
//
LOCUS       DAORVN010000276         2595 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_38194_length_2595_cov_2.811024, whole genome shotgun sequence.
ACCESSION   DAORVN010000276 DAORVN010000000
VERSION     DAORVN010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2595
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38194_length_2595_cov_2.811024"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..662)
                     /locus_tag="PKV23_06370"
     CDS             complement(<1..662)
                     /locus_tag="PKV23_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HQX85096.1"
                     /translation="MSEVISTDAVIIGAGPCGLFAIFELGLLDIKCHVVDILDRAGGQ
                     CAELYPEKPIYDIPALPIVTGSELTQRLLDQAKPFHPQFHFNQMVTELIKHGDNDFEL
                     RTDGGMVFRSKIVVIAAGGGSFQPKKPPIPGIDAYEGTSVFYSVKKMETFRGKDILIV
                     GGGDSALDWTNNLAPIARSLTLLHRRDEFRAAPDSVKKMRELVAAGKMQIKLGQVTGL
                     TGA"
     gene            complement(680..1000)
                     /locus_tag="PKV23_06375"
     CDS             complement(680..1000)
                     /locus_tag="PKV23_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HQX85097.1"
                     /translation="MAKITFIQHNGTEQTVDGIPGMTVMEAAIKNMVPGIDADCGGAC
                     ACATCHVYVEAEWLDKVGKRAAMEEDMLDFAFDVKDSSRLSCQIKISDGLNGLRVRVP
                     EKQF"
     gene            complement(1068..1439)
                     /locus_tag="PKV23_06380"
     CDS             complement(1068..1439)
                     /locus_tag="PKV23_06380"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HQX85098.1"
                     /translation="MQAAASKSGRTKPVPVIFDRSHLAQYTLDSPELEREIVGLFVAQ
                     LPAILDRLQNGDSREDWRVATHTLKGSALAIGACKIGDLARKLEPVNSPEQDAKRKKL
                     LSGLVQAVNEFDEMARQLFPS"
     gene            1749..>2595
                     /locus_tag="PKV23_06385"
     CDS             1749..>2595
                     /locus_tag="PKV23_06385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85099.1"
                     /translation="MASNTVETRRRDKRSAERQAESRRGPSLEATPLRGRPANENDPG
                     TSMMMARLHRRPSYVAYYVAIPAILAWVFVWFMAYSPVIAEQALPQLVASVALLTFPV
                     IIMGIMAYFYSRAQQLRQVSEVLMQSAMRLIRPQDIATEGLTSIAQAVRSEVDLLVGG
                     VEHAVQRATVLEEIVHKEISAIERAFGGNEDRIRTLVTGLENQRAALHQASLVVGNES
                     APLLARLEINTQNLDSVVNSAQSTLVRFEQGLKNSTSELARTIDEVSARATVAVIPCH
                     QTRHHS"
BASE COUNT          530 a          774 c          721 g          570 t
ORIGIN      
        1 gcccccgtaa ggccggtcac ctggccgagc tttatttgca ttttgcctgc ggccacaagc
       61 tcccgcattt ttttcacgct gtcgggtgcc gcccggaact catcgcgccg atgcagcagc
      121 gtcagactcc tggcaatggg tgcaagatta ttagtccagt ccagagccga atcgccgccg
      181 ccgacgatga gaatgtcctt gccgcggaaa gtctccatct tcttgacgga ataaaacacc
      241 gaggtgcctt cataggcgtc aatgccggga atgggcggct tcttcggctg gaaggaaccg
      301 ccgcccgcgg cgatgaccac gattttgctt ctgaagacca tgccgccatc ggttcgcagc
      361 tcgaaatcat tgtctccgtg cttgatcaat tcggtgacca tctggttgaa atgaaactgc
      421 ggatggaacg gctttgcctg gtcgagcagg cgctgcgtga gttcagatcc cgtgacgatc
      481 ggcagggccg gaatgtcgta gatcggtttt tccggataaa gctccgcgca ctggccgcca
      541 gcccggtcaa gaatgtcaac aacgtggcat tttatgtcca gcaggcccag ttcgaagatt
      601 gcaaacaggc cgcaagggcc tgccccaatg atcacggcat cggtggaaat gacttcagac
      661 atctcaaact ccagtaaggt cagaattgct tttcgggaac ccggacgcgc aagccgttga
      721 gcccgtcgct gatcttgatc tggcaggaaa gccggctgga atcttttacg tcgaaggcaa
      781 aatccagcat gtcttcttcc atggcggcgc gcttgcccac cttgtccagc cattcggctt
      841 ccacatagac gtggcaagtg gcgcaggcgc acgccccgcc gcaatccgca tcaatgccgg
      901 gcaccatgtt cttgatcgcc gcctccatca cagtcatgcc cggaatgccg tcaacggtct
      961 gttccgtgcc attgtgctgg atgaaggtaa ttttcgccat gtcgcgtccg caatctcgtg
     1021 ttcgttttat cgaacgctac ataggatata aagaattgaa aggcaagtca gctggggaaa
     1081 agttgccggg ccatttcatc aaattcattt acggcctgaa ccagccctga caggagtttt
     1141 ttgcgcttgg cgtcctgctc cgggctgttg accggctcca gctttctggc caggtcgccg
     1201 attttgcagg cgccgatggc cagcgccgat cccttgagag tatgcgtggc tacgcgccaa
     1261 tcttcgcgcg aatcgccatt ctgcagacga tccagaattg ccggcaattg ggccacgaac
     1321 agtccgacga tctcgcgctc gagctcgggg ctgtccaagg tgtattgcgc cagatggctc
     1381 cggtcaaaaa tgaccgggac tggttttgtc cgccccgatt tgcttgctgc cgcttgcacc
     1441 actgcgccgt ccctgcttca atttggagca tcaaactcct ggggtgttat aggtcgcgag
     1501 cgtaaatggg cagttttctg aatcgtttca attcgatcca taattttaac caaaccttta
     1561 attatacttg cggatagcgc cagaacgggt tctatcatgg ttaccaatag gttaaggtag
     1621 gagtcttggt gggaggtatg gaaggggccg gtctgcatcg gaaacgcaga ttctttcatg
     1681 aaatcaagca tcttccggca gcctgagggg ctgttggaaa attatgtata agaatgtggg
     1741 taaatcgtat ggcaagtaac acggtggaaa cgcgtcgcag ggacaagcgt tcggcggaac
     1801 ggcaggctga atcaagacgc ggcccgtctc tggaggcaac acccttgcgt ggccggcctg
     1861 ccaacgaaaa cgaccccggc accagcatga tgatggccag actgcatcgg cggccgtctt
     1921 acgtcgctta ctacgtcgcc ataccggcaa tcttggcctg ggtattcgtc tggtttatgg
     1981 cctattcacc cgtgatcgcc gagcaggccc tgccgcagct tgtggcgtcc gtggccctgc
     2041 tcactttccc tgtcatcatc atggggatca tggcctattt ctattcgcgc gcccagcagt
     2101 tgcgtcaggt ttcggaagtc ctgatgcagt cggccatgcg cctgatccgc ccccaggaca
     2161 ttgccaccga gggcctcacg tccatcgccc aggcggtccg ctcggaagtt gatttgctcg
     2221 tcggcggcgt cgaacacgcg gtccagcgcg ccaccgttct ggaggaaatc gttcacaagg
     2281 aaatatcggc catcgagcgc gccttcggcg gcaatgagga ccgcatccgt accctggtga
     2341 ccggactgga aaaccagcgc gccgccctgc accaggcctc cctcgtggtg ggcaatgaat
     2401 cggccccctt gctggcccgc ctggaaatca acacgcagaa tctcgacagc gtcgtcaaca
     2461 gcgcccagtc caccctcgtc aggtttgaac agggcctcaa gaattcgacc agcgaactcg
     2521 cccgcaccat tgatgaagtt tccgcccgcg ccaccgtcgc cgtcataccg tgccaccaga
     2581 cccgccacca ctcgc
//
LOCUS       DAORVN010000277         2593 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_38239_length_2593_cov_3.118597, whole genome shotgun sequence.
ACCESSION   DAORVN010000277 DAORVN010000000
VERSION     DAORVN010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2593)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2593)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2593
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38239_length_2593_cov_3.118597"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..515
                     /locus_tag="PKV23_06390"
     CDS             <1..515
                     /locus_tag="PKV23_06390"
                     /inference="COORDINATES: protein motif:HMM:NF012703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HQX85100.1"
                     /translation="PLTILQIDAHIDWRDERYGEKFGFSSTMRRASEQAHVWRIVQVG
                     ARGLGSAREAEVRHAKQWGAHIFTSRHVHHNGLKDILDTIPPDSDCLITLDCDALDTS
                     EMPAVAHPSPGGLTYMQVVDLIAGVAAKARIAGFTMVEFAPKKDRDGASAYTAARIAA
                     NIIGHIARQI"
     gene            620..1438
                     /locus_tag="PKV23_06395"
     CDS             620..1438
                     /locus_tag="PKV23_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012044679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase/chromate resistance protein"
                     /protein_id="HQX85101.1"
                     /translation="MSAPNAIKSDKLARLIGTASTPAIIDVRTQEDFEADPRLIPGAM
                     KRSHQTVSEWGKAHAGKPAIVVCHHGAKLSEGTAAWLRQLGADAVSLEGGFVEWDTAK
                     LPLVPVAKVPKRDAEGRTIWVTRARPKIDRIACPWLIRRFVDPAAVFLFVAPQEVAAV
                     ADRFNATAFDIENTHWSHRGDLCTFDVMVEEFGLATEALLRLATIVRAADTARLDLAP
                     EAAGLLAASLGLSRMYADDLEQLEAGMGLYDAFYRWCRDATEETHNWPTNKVKP"
     gene            1435..>2593
                     /gene="chrA"
                     /locus_tag="PKV23_06400"
     CDS             1435..>2593
                     /gene="chrA"
                     /locus_tag="PKV23_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008835600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HQX85102.1"
                     /translation="MSPSPTFAQATKVWARIGCLSFGGPAGQIALMHREIVDERKWLS
                     EQQFLSALNFCMLLPGPEAMQLATYSGWRLHGVRGGLVAGLLFVLPGALVVLALSMLY
                     AAFGNLPWVEAIFFGVKAAVLAIVIEALLRVARRALKGTLDWLIAGVAFLALYCFDVP
                     FPLIIIAAGLVGFLRSSAADTSPATLRPSFTKLFSTSATWAAIWLMPLALLAITLGMD
                     HVLTKIGIFFSQLAVVTFGGAYAVLSYMAQSAVESHGWLTAPEMIDGLALAETTPGPL
                     ILVTQFVGYLAALRGMGSVWGGLAGAAITLWMTFAPCFLWIFAGAPYIEHISRMPRLS
                     GALAAITAAVVGVILNLSLWFGLHVLFARVNRGEGPFKPWLPDWQTLDIAAL"
BASE COUNT          454 a          892 c          734 g          513 t
ORIGIN      
        1 cgccgctcac catcctgcag atcgacgccc atatcgactg gcgggacgaa cgctatggcg
       61 aaaaattcgg cttttccagc accatgcgcc gcgcctcgga acaggcccat gtctggcgca
      121 tcgtccaggt gggagcccgc ggcctcggca gcgcccggga agccgaagtg cgccatgcca
      181 agcaatgggg cgcccatatc ttcacctcgc ggcacgttca ccacaacggc ttgaaagata
      241 tcctcgacac cataccgccg gactccgatt gcctgatcac cctggattgc gacgcgctgg
      301 acacttcgga aatgcccgcc gtcgctcatc cgtcgcccgg cggcctgacc tacatgcaag
      361 tggtggacct catcgcaggc gttgccgcca aggcccgcat cgccggtttc accatggtgg
      421 agtttgcacc gaagaaggac cgcgacggcg cttcggccta taccgccgca agaattgccg
      481 caaacatcat tggccatatc gcaaggcaga tttgacttag ccgaattctt tgctattgat
      541 cagcgcaggg gaaagcgatc tcccctcaag gcgacatgcc caagaatcgc gtcccatcaa
      601 cattcaagag ggcgcaaaca tgtctgcacc aaacgcgata aaatccgata agctcgcacg
      661 cctcatcggc acggcttcga caccggcgat catagatgtc cgcacgcaag aggacttcga
      721 ggctgaccca cgcctcattc ccggcgcaat gaaacgaagc caccaaaccg tttccgaatg
      781 gggcaaggcc catgccggaa aacccgctat cgtcgtctgc catcacgggg caaaactcag
      841 cgagggaacc gccgcatggc tccgccagct cggtgctgat gccgtatcgc tcgaaggcgg
      901 cttcgtggaa tgggatacgg caaaactgcc gctggttccc gtggcgaaag tccccaagcg
      961 tgatgctgaa ggccgcacca tttgggtcac ccgcgcccgg cccaagattg accgcattgc
     1021 ctgcccctgg ctcatccggc gatttgtcga tcccgccgcc gtgtttctgt ttgtggcccc
     1081 gcaggaagtg gccgccgtcg ccgaccgctt caatgccacc gccttcgata tcgagaacac
     1141 ccactggagc catcgcggag acctttgcac tttcgatgtg atggtcgagg aatttggtct
     1201 cgccactgaa gcgctgctgc gcctcgccac cattgtccgc gccgccgata cggcacggct
     1261 tgatctggcg ccggaagccg caggccttct cgccgcatcg cttgggctct cgcgcatgta
     1321 cgccgatgat ctcgaacagc ttgaggctgg gatggggctc tacgacgctt tctaccgctg
     1381 gtgccgggat gcgaccgagg agacccacaa ttggcccacc aacaaggtga agccatgagt
     1441 ccttcgccaa cgtttgccca agccacgaaa gtctgggccc gcatcggctg cctctccttc
     1501 ggcgggccgg cgggccagat cgccctcatg caccgcgaaa tcgtcgatga gcggaaatgg
     1561 ctgagcgagc agcaatttct ctcggccctc aatttctgca tgctgctgcc ggggccggaa
     1621 gccatgcagc tcgccaccta ttcagggtgg cgcctgcatg gagtcagggg cggattggtg
     1681 gcaggcctgc tcttcgtatt gcccggcgcg ctcgtcgtgc tggccctttc catgctctat
     1741 gccgccttcg gcaacctgcc ctgggttgaa gcgattttct tcggcgtcaa ggccgcagtc
     1801 ctcgccatcg tcatcgaggc gctgctccgc gtggcaaggc gcgccctcaa aggcacgctt
     1861 gattggctca tcgccggcgt ggccttcctc gcactttatt gtttcgatgt gcccttcccg
     1921 ctcatcatca tcgctgccgg cctcgtgggc ttcctccgca gcagcgcggc agacacgtca
     1981 ccggcaaccc tgcggccaag cttcaccaaa ttgttttcaa cctccgcaac ctgggccgcc
     2041 atctggctca tgccgcttgc acttctcgca atcaccttgg gcatggatca cgtgttgacc
     2101 aagatcggca ttttcttttc tcagcttgcc gtggtgacct tcggcggcgc gtatgcggtg
     2161 ctctcctaca tggcccagtc cgccgtggag agccacggct ggctcaccgc ccccgaaatg
     2221 atcgatggcc tggcgctggc cgaaaccacc cccggccccc tgatcctcgt cacgcaattc
     2281 gtgggctacc tcgcggcctt gcgcgggatg ggcagcgtct ggggcgggct ggccggtgcg
     2341 gccattaccc tgtggatgac ctttgccccc tgcttcctct ggatcttcgc cggcgccccc
     2401 tacatcgagc atatcagccg catgccccgg ctttccggcg cgctcgccgc catcaccgcc
     2461 gccgtggtcg gcgtcattct taacctttcc ctgtggtttg gcctgcacgt gctgtttgcc
     2521 agggttaacc gcggggaagg cccattcaag ccctggctgc ccgactggca aaccctcgac
     2581 attgccgccc tgg
//
LOCUS       DAORVN010000278         2588 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_38359_length_2588_cov_3.541650, whole genome shotgun sequence.
ACCESSION   DAORVN010000278 DAORVN010000000
VERSION     DAORVN010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2588
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38359_length_2588_cov_3.541650"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1169)
                     /locus_tag="PKV23_06405"
     CDS             complement(<1..1169)
                     /locus_tag="PKV23_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018063513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQX85103.1"
                     /translation="MTPAIELIGIDKRFGAVHANKDITLEIAQGSIHGIIGENGAGKS
                     TLMSILFGFYSADKGEIKIKGRPVKIKSSSDAISFGIGMVHQHFMLVDPFTVLENVVL
                     GAEGGRLIGPALAKAREELTRLARDYELEVDPDAIVGELPVGLQQRVEILKALFKGAE
                     TLILDEPTGVLTPDEADHLFRILGQLRDQGKTVILITHKLREIMAITDHVSVMRRGEM
                     VATKETKKTSVEELAELMVGRRVLLRVKKGKAKPGQVVLDVKNLTWKDRKGVARVDNV
                     SFTVRSGEIVGIAGVSGNGQSELLELISGISKPSSGQIIFEGKDIGILGDPRHVREEG
                     LAHVPEDRQHRGLIASFDASESGILGWQYDESLGRGFRLDRQAVVARSKKDMLEF"
     gene            complement(1232..2230)
                     /locus_tag="PKV23_06410"
     CDS             complement(1232..2230)
                     /locus_tag="PKV23_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006203175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HQX85104.1"
                     /translation="MIRKLLATAALTAMMAASAFAADDKPAIVFDIGGKFDKSFNESM
                     YNGSEKFKAESGTAYGEFEIANEAQREQAIRNFADQGYSPIIAAGFAQASALEKVAKE
                     YPDLKFAIVDMVVELPNVQSIVFKEQEGSYLVGMMAAMASKSGKVGFVGGMDIPLIRR
                     FGCGYVQGVKAAKADATVFQNMTGDTGAAWNDPVKGGEITKGQMAQGADVVYAAAGAT
                     GLGVLQAAADGGALSIGVDANQNYLHPGKVLTSMLKRVDVAVYNVMKSGKDGTFKPGI
                     LELGLAEDGVGYAIDEHNKALVTAEMAAAVEKAKADIIAGTVKVHDYMSDNKCPVE"
     gene            complement(2317..>2588)
                     /locus_tag="PKV23_06415"
     CDS             complement(2317..>2588)
                     /locus_tag="PKV23_06415"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQX85105.1"
                     /translation="ELAPRGVRLNAICPCDVETPMLDFQAREFGGGDPQGYIARLRSI
                     YPQGARQRFAKPAEIAAFIFAIASPRLAPITGAALPIDFGTTAGR"
BASE COUNT          508 a          742 c          766 g          572 t
ORIGIN      
        1 tcaaattcca gcatgtcctt tttgctgcgc gcaacaaccg cctggcggtc caggcgaaag
       61 ccgcggccca gggactcatc atactgccaa cccagaatgc cggattcaga tgcgtcaaaa
      121 gaggcgatga ggccgcggtg ctggcggtct tccgggacat gggcgaggcc ctcctcgcgg
      181 acatggcgcg gatcgccgag aatgccgata tccttgcctt caaaaatgat ctggcctgac
      241 gagggttttg aaatgccgct gatcagttcg aggagttccg actggccatt gccggaaaca
      301 ccggcgatgc cgacgatttc accgctgcgc acggtgaagg atacattgtc aacgcgggcc
      361 acacccttgc ggtctttcca ggtgaggttc ttcacatcga gcaccacctg gccgggcttg
      421 gccttgccct tcttcacgcg cagcagcacg cggcggccca ccatgagttc ggccagttct
      481 tccaccgagg ttttttttgt ttccttggtc gccaccattt cgccacggcg catgaccgag
      541 acatggtcgg tgatggccat gatctcgcgc agcttgtggg tgatgaggat cacggttttg
      601 ccctggtcgc ggagctggcc cagaatgcgg aacagatggt cggcctcatc gggggtgagc
      661 acgccggtgg gttcatcaag gatcaaggtt tcagcgccct tgaagagcgc tttcagaatc
      721 tcgacgcgct gctggaggcc cacgggcaat tcaccgacga tggcatcggg atctacttcc
      781 agctcgtaat cgcgtgccag ccgggtgagt tcctcgcggg ctttggcaag ggcggggcca
      841 atgagccttc cgccttcggc gccaagcacg acattttcaa gaacggtgaa gggatcaacc
      901 agcatgaagt gctggtggac catgccgatg ccgaagctta tggcatcaga cgaagatttt
      961 atcttaacgg gccggccctt gattttgatc tcgcccttgt cggcgctgta gaatccgaag
     1021 aggatcgaca tcagggtgga cttgccggcg ccattttcgc cgataatgcc atgaatggac
     1081 ccctgggcga tttccaaggt tatgtccttg ttggcgtgaa cagcgccaaa gcgtttgtcg
     1141 ataccgatga gttcaatggc tggggtcatg gttatttcaa tgaaaccctc tcccgcgatg
     1201 ggagagggtc aattgtcaca aggttgaccg attattcaac cgggcacttg ttgtcggaca
     1261 tgtagtcatg gaccttcacg gtgccggcga tgatgtcggc cttggccttt tcgacggcgg
     1321 ctgccatttc ggccgtgacc agggccttgt tgtgctcgtc aatggcgtaa cccacgccat
     1381 cctcggccag gccgagctcc agaatgccag gcttgaaggt gccgtccttg ccggacttca
     1441 tcacgttata gactgcgaca tccacgcgct tcagcatgga ggtcaaaacc ttgccggggt
     1501 gcagatagtt ctggttggcg tcaacgccga tcgacagcgc gccgccatcg gcggcggcct
     1561 gcaatacgcc aaggcccgtg gcgccagccg cggcgtagac cacgtcagca ccctgggcca
     1621 tctggccctt ggtgatttcg ccacccttca ccggatcgtt ccaggcagca ccggtgtcac
     1681 cggtcatgtt ctggaaaacc gtggcatcag ccttggcggc tttcacgccc tggacatagc
     1741 cgcagccaaa gcggcggatc aacggaatgt ccatgccgcc gacaaagccg accttgccag
     1801 atttggacgc catggcggcc atcatgccaa cgagataaga gccttcctgc tccttgaaca
     1861 caatggactg cacgtttggc aattcaacga ccatgtccac gatggcaaac ttgagatcgg
     1921 gatattcctt ggcgactttt tcaagggccg aagcctgggc aaagcccgcg gcgatgatgg
     1981 gactgtagcc ctggtcggcg aaatttcgga tggcctgttc acgctgggct tcgtttgcga
     2041 tttcaaattc gccatatgcg gtgcctgact cagccttgaa tttttccgaa ccgttgtaca
     2101 tgctttcatt gaaggacttg tcgaacttgc cgccaatgtc aaaaacgatg gccggcttgt
     2161 cgtcggcggc gaatgcggac gccgccatca ttgctgtcaa tgcagcggtc gcgagtaatt
     2221 tcctgatcat aaacatctcc ctgatttttt tgaccggctg atcaagcctt gacggcaatt
     2281 gacgccaaat tcctgtcatt tgcaagtggc gaaccctcat cgcccggcgg tggttccgaa
     2341 gtcgatgggg agtgctgcac cggttatcgg ggccaatcgc ggcgaagcga tggcgaagat
     2401 aaaagctgct atttccgcgg gttttgcgaa gcgctggcgg gccccttgcg gatagattga
     2461 acgcagtctg gcgatatagc cttgcggatc gccgccgccg aattcgcggg cctggaagtc
     2521 cagcatgggg gtttccacat cgcaggggca aatggcgttt aaccgcacgc ctctcggcgc
     2581 gagttcga
//
LOCUS       DAORVN010000279         2581 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_38553_length_2581_cov_2.547110, whole genome shotgun sequence.
ACCESSION   DAORVN010000279 DAORVN010000000
VERSION     DAORVN010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2581
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38553_length_2581_cov_2.547110"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..751
                     /gene="rsmA"
                     /locus_tag="PKV23_06420"
     CDS             <1..751
                     /gene="rsmA"
                     /locus_tag="PKV23_06420"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012114824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HQX85106.1"
                     /translation="DLNLTRRIARSAAPLEGFTIVEVGPGPGGLTRALLMEGAKQVIA
                     IERDARTLPALAEISAAYPGRLSVIADDALAMDWPAVVTGPAKIVANLPYNVATALLT
                     GWLTQNAWPPWFSSLTLMFQKEVAERIVAKPGGKDYGRLSVLCQWRCDAKKLFDVNRS
                     AFTPAPKVTSSIVQLNPRQSFDVPCDVKDLEKVTAAAFGQRRKMLRSSLRAAFETPEI
                     ILAELGINPELRAEQLQVNDFARLAKAFGEL"
     gene            complement(752..1348)
                     /gene="gmk"
                     /locus_tag="PKV23_06425"
     CDS             complement(752..1348)
                     /gene="gmk"
                     /locus_tag="PKV23_06425"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015667267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HQX85107.1"
                     /translation="MSSPSGAGKTTLSRKLLAADKNVTMSVSVTTRPARPGETDGKDY
                     HFISKERFAQMRDRKELLEWAEVFGNYYGTPRKPVDEALARGRDVLFDIDWQGTQQIA
                     QVMMEDLVRIFILPPSSDELQNRLINRAQDSSTVVAKRMAEASNEISHWAEYDYVIVN
                     DELEDSHAQVTAILTAERLRRRRRVGLTEFVRQLTKGL"
     gene            complement(1395..2282)
                     /locus_tag="PKV23_06430"
     CDS             complement(1395..2282)
                     /locus_tag="PKV23_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HQX85108.1"
                     /translation="MALSSMTGFAQSSGEKDGLHWQWEIKSVNGKALDLRCRLPTGFE
                     ALEAIARSTLAQHIKRGNLQVTLSTSGSVSREAVVVNEAVLEQVLAIAEKLRDRIGGV
                     PLSAVDLMGLRGVLDVVQSTQTEAEMAARLADIAGSLEKAAVALDAARRAEGARLSTI
                     ISAQLEKIEGLVIAARDCPARSVDAVKQRLQEQIVRLLDTGAGFDKDRLHQEAVLIAT
                     RADIQEELDRLFAHLEAARALMASPEPAGRKFDFLAQEFNREANTLCSKALDKTLTAI
                     GLELKTVIDQMREQVQNIE"
     gene            2415..>2581
                     /locus_tag="PKV23_06435"
     CDS             2415..>2581
                     /locus_tag="PKV23_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006697616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HQX85109.1"
                     /translation="MGKRIVFTGGSGKAGRHVVPWLRARGYDILNLDLKPLDCPGVNT
                     LITDVTDSGQV"
BASE COUNT          469 a          788 c          778 g          546 t
ORIGIN      
        1 cgacctgaac ctcacgcggc gcattgcgcg cagtgcagcg ccgctggaag gtttcacaat
       61 cgtggaagtt ggccccggcc ccggcggact gacacgggcg ctgctgatgg aaggggcgaa
      121 gcaggtgatc gccattgaac gcgatgcaag aaccctgccc gcccttgccg aaatttccgc
      181 ggcctatccc ggcaggcttt cggtcattgc cgatgatgcg cttgccatgg attggcccgc
      241 ggttgttaca ggcccggcga agatcgtcgc caacctgccc tataatgttg ccaccgccct
      301 gctcaccggc tggctgacgc aaaacgcctg gccgccgtgg ttttcatcct tgacgctgat
      361 gttccagaag gaagttgccg aacgcatcgt ggcaaagccc ggcggcaagg actacggcag
      421 gctgtcggtg ctttgccaat ggcgctgcga cgcgaagaag ctgtttgatg tcaatcgcag
      481 cgccttcacg cccgccccca aagtcacgtc cagcatcgtt caactcaatc ccaggcagtc
      541 ctttgacgtg ccttgtgatg tgaaggatct tgagaaagtg acggctgcag cgttcggtca
      601 gagacggaaa atgttgcgct cgagcctgcg cgcggcgttc gaaacgccgg aaatcattct
      661 cgctgagctt ggcatcaatc cggaactgcg ggccgagcag ctccaggtca atgatttcgc
      721 tcgtctcgcg aaagccttcg gagaactttg attacaaacc cttggtgagt tgtcggacaa
      781 attcggtcag gcccacgcgg cgcctgcggc ggagcctttc ggcggttagg atggctgtca
      841 cctgtgcatg ggagtcttca agctcgtcat tcacaatcac ataatcatat tcggcccagt
      901 ggctgatttc gttcgaggct tccgccatgc gctttgcgac aacggtggag gaatcctggg
      961 cgcggttgat gagccggttt tgcagttcat cggatgaggg cgggaggatg aatatccgca
     1021 ccaggtcttc catcatcacc tgggcgatct gctgggttcc ctgccagtcg atatcgaaca
     1081 gcacatcgcg gcccctggcc agtgcctcgt ccaccggctt gcgcggggtg ccataatagt
     1141 ttccaaagac ctcggcccat tccagcagtt ccttccggtc gcgcatctgg gcgaagcgct
     1201 ccttgctgat gaaatgataa tccttgccat cagtttcgcc gggccgggcg ggacgggtgg
     1261 tgaccgacac ggacatggtg acattcttgt ccgcggcgag gagcttgcgc gacagcgtcg
     1321 tcttgccggc ccctgatggc gaggacatga ctagcagcag gccccttctg gcaatctcgg
     1381 ttctttgcac caccctattc aatgttttgc acctgttcgc gcatctgatc gatgactgtt
     1441 ttcagctcaa ggccaatggc ggtcaaggtc ttgtccaggg cttttgagca cagcgtattg
     1501 gcttcccggt tgaattcctg ggccagaaaa tcaaacttgc ggcctgccgg ttcgggcgag
     1561 gccatcaggg cccgggccgc ctcgagatgg gcaaacaacc ggtccagttc ctcctggatg
     1621 tcagcgcggg tggcgatcag gactgcttcc tgatgcagcc ggtccttgtc aaagccagcg
     1681 ccggtatcga gcagcctgac gatctgctcc tgcaggcgct gcttcacggc atccaccgac
     1741 cgcgccgggc aatcgcgggc ggcgatcacc aagccttcaa ttttttcaag ctgggcggaa
     1801 atgatggtgg aaagccgcgc cccttcggcg cggcgcgcgg cgtcgagggc aacggcggcc
     1861 ttttcaagcg agccggcaat atcggccaga cgtgccgcca tctcggcctc ggtttgggtg
     1921 gattgcacca cgtccaacac gccgcgcagg cccatgagat ccacggcgct gagcggcacg
     1981 ccgccaatcc ggtcgcggag tttctcggca atggcgagga cctgttcgag gacggcttca
     2041 ttcacgacaa cggcttcccg cgaaacgctg ccgctggtgc tcagcgtcac ctgcaggttt
     2101 ccgcgcttga tgtgctgcgc cagggtgctg cgggcgatgg cctcgagggc ctcaaagcct
     2161 gtcggcaggc ggcagcgcag gtccagcgcc ttgccgttca cgctcttgat ttcccattgc
     2221 cagtgcaggc catccttttc gcccgagctt tgtgcaaacc ccgtcatgct cgaaagcgcc
     2281 atgtggtccc cgttgccaga atcaatccgt ggaaagtcta acaggtggca gggctctact
     2341 ccactttagt cctcgccagc gccctatttg gccttgtatt atgcgccttt ccactcttca
     2401 gcaaaggata atccatgggc aaacgcattg ttttcaccgg cggttcaggc aaggcggggc
     2461 ggcatgtggt gccgtggctc agggcccggg gctatgacat cctcaacctt gacctcaagc
     2521 cgctggattg cccgggggta aacaccctga tcaccgatgt gaccgacagc ggacaggttt
     2581 t
//
LOCUS       DAORVN010000280         2577 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_38666_length_2577_cov_2.996035, whole genome shotgun sequence.
ACCESSION   DAORVN010000280 DAORVN010000000
VERSION     DAORVN010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2577
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38666_length_2577_cov_2.996035"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            179..2236
                     /locus_tag="PKV23_06440"
     CDS             179..2236
                     /locus_tag="PKV23_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011504303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HQX85110.1"
                     /translation="MDSNLGRRSQGSRCVALVGPYLSGKTTLLEAILFRTGATRRQGK
                     ISEKSTIGDASPEARGHGMSVELNVATTEYLGERYTFLDCPGSIEFNQDIGAALVGCD
                     VAVVVCEADEKKIPALQLILKRLDEFNLPRIVFINKIDHSNTTPHTVLEFMQPASSKP
                     LVLRQLPIWSNGIVTGFVDLALERAYVYREHAESTVVEIPAEMKPAEKNERFAMLEKL
                     ADHDDELLEQLLADMEPPRDRVFADLSRELAEGQVTPVFFGSAEHGNGIGRLLKALRH
                     EAPTVEATAKRLGLKSGAGATAQVLKTFHTASGGKLSIARVLAGELPDGAVVYGASGQ
                     EARVAGVFSMLGATPTKVPKATLGDTVALGRLEKINTGETISTVKGATKQLAVLTPAP
                     GVYGVAVSVTDRKDEVKLTSSIAKLIEEDPSLSLEHNANTHEMVLWGQGEMHLRVALE
                     RLQGKFGVHAKSQPRRIGYKETIKKSVQLRGRHKKQSGGHGQYGDVTVEIKPLPRGSG
                     FVFTDTITGGVVPKQYIPAVEAGVREWMVQGPLGFSVVDFTVNLSEGSYHDVDSSEMA
                     FKLAARLAMTEGMPQCSPVLLEPIVAVDIHVPSDATSRVNQIVTGHRGQLLGFDGREG
                     WPGWDTVKAHLPESEIGSLIIELRSATSGVGTFVFSHDHMAELVGRSAEQVVAARKAQ
                     MAG"
     gene            complement(2246..>2577)
                     /locus_tag="PKV23_06445"
     CDS             complement(2246..>2577)
                     /locus_tag="PKV23_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007757565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HQX85111.1"
                     /translation="NQHDLDKIMSHFADDCVLEMPRGNQPWGSRSEGRESVRKALATR
                     FEGLPDVHYGNPQHFVDAGANTGISKWTLTGTRRDGQRIEVCGCDFYTFRHGKVTRKD
                     SYWKIVE"
BASE COUNT          523 a          708 c          864 g          482 t
ORIGIN      
        1 catttcaaag atgcgctggc ggagtgtcgg ggttgtatcc atgcgctgaa tctaaactac
       61 aaaacttgcc tcttgccaat gttcataatt agagcaagca tgggtgcgtc ggatgcgccc
      121 agagacgggg ccccaacccc gcacacggac ggtccttgac agtttgggag gatcaaacat
      181 ggactccaac ctcggcagaa ggtcccaggg ctcgcgttgt gtggcgctcg tgggccccta
      241 tctcagcggc aagacaaccc ttctcgaagc cattctattc cgcaccggcg ccacgcggcg
      301 gcaaggcaaa atctccgaaa aatccaccat aggcgatgca tcgccggaag cgcgcgggca
      361 cggcatgagc gttgagctca atgtggccac aacagaatac ctgggagagc gctatacgtt
      421 cctggattgc ccgggctcga tcgaattcaa ccaggacatt ggcgcagccc tcgtcggctg
      481 cgacgtggcg gtggtggttt gcgaggcgga cgagaaaaaa atcccggccc ttcagctcat
      541 tctcaagcgg cttgatgaat tcaacctgcc gcgcatcgtt ttcatcaaca agatcgacca
      601 ttcgaatacg acgccgcaca cggtactgga attcatgcag ccggccagca gcaagccgct
      661 ggtgctgcgg cagctgccga tctggagcaa tggcatcgtg accgggtttg tcgacctggc
      721 gctggagcgg gcctatgttt accgcgagca tgcggagagc acggtggttg aaattccggc
      781 cgagatgaag ccggcagaaa agaacgaacg ctttgccatg ctggaaaagc ttgccgacca
      841 tgacgatgaa ttgctggagc aactgcttgc cgacatggag ccgccgcgcg accgggtgtt
      901 cgccgatctt tcgcgcgagc ttgccgaagg gcaggtgacg ccggtgttct ttggttcggc
      961 cgagcatggc aacggcatcg gccgtttgct gaaggcgctt cgccatgagg cgccaacggt
     1021 tgaggcaacg gccaagcgcc ttgggctcaa gagcggggcg ggggccacgg cacaggttct
     1081 gaagaccttc cacacggcca gcggcggaaa gctttccatc gcccgggtgc tggcgggcga
     1141 gctgccggac ggcgcggtgg tttatggcgc cagcggccag gaggcgcggg ttgccggggt
     1201 gttttccatg ctgggcgcca cacccacgaa ggttcccaag gccacgctgg gcgatacggt
     1261 ggcccttgga cggctggaga agatcaacac gggcgagacg atctcgacgg tgaagggcgc
     1321 cacaaagcaa ctggcggtgc taacgccggc gccgggggtt tacggggttg cggtgtcggt
     1381 gaccgaccgc aaggatgaag tgaagctcac ctcatccatc gccaagctga ttgaagagga
     1441 tccttcgctg tcgctcgaac acaacgccaa tacgcatgag atggtgctgt gggggcaggg
     1501 ggagatgcat ttgcgcgtgg cgctggagcg gctgcagggc aagttcgggg tgcacgccaa
     1561 atcccagccg cggcgcatcg gctacaagga gacaatcaag aaatcagtgc aattgcgcgg
     1621 gcggcacaag aagcaatcgg gcgggcatgg ccagtatggc gacgtgacgg tggaaatcaa
     1681 accgctgccg cgcggctcgg gctttgtgtt caccgacacg atcaccggcg gggtggtgcc
     1741 caagcagtat attccggcgg tggaagccgg ggtgcgggaa tggatggtgc aggggcccct
     1801 gggattttcg gtggtggatt tcaccgtcaa tctttccgaa ggctcctatc atgatgtgga
     1861 ctcatcagaa atggcgttca aactggcggc aaggctggca atgacggaag gcatgccgca
     1921 gtgttcgccg gttctgctgg agcccattgt ggcggttgac atccatgtgc cgagcgatgc
     1981 gacctcacgg gtgaaccaga ttgtcaccgg gcaccgcggg cagcttcttg gctttgacgg
     2041 gcgcgagggc tggccgggct gggacacggt aaaggcgcat ctgccggaat cggaaattgg
     2101 ctcgctgatc attgagctgc gctcggctac atcaggagtg ggaaccttcg tgttcagcca
     2161 cgatcacatg gctgagctgg tggggcgcag cgccgaacag gtggtggcgg caaggaaggc
     2221 gcagatggcg ggatagggag ccttcttact ccacgatttt ccagtaggag tccttgcggg
     2281 tgaccttgcc atggcgaaaa gtgtagaagt cgcagccgca gacttcgatg cgctgcccgt
     2341 cacggcgggt tccggtcagg gtccattttg atatccccgt gttggcgccg gcatcgacga
     2401 agtgctgcgg gtttccatag tgaacatcgg gcaggccctc gaagcgggtt gccagggcct
     2461 tccggacact ctcacggcct tcggaacggg atccccaggg ctgattgccg cgcggcattt
     2521 ccaggacgca gtcatcggca aaatgactca tgattttgtc aaggtcatgc tgattaa
//
LOCUS       DAORVN010000281         2563 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_39023_length_2563_cov_3.563796, whole genome shotgun sequence.
ACCESSION   DAORVN010000281 DAORVN010000000
VERSION     DAORVN010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2563
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39023_length_2563_cov_3.563796"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            192..2228
                     /locus_tag="PKV23_06450"
     CDS             192..2228
                     /locus_tag="PKV23_06450"
                     /inference="COORDINATES: protein motif:HMM:NF014896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HQX85112.1"
                     /translation="MTTSALAQEDPYLWLEEVMGDRAISWVKEQNAKSQKLLEASPQF
                     AAIRSKSLAVLNSRARIPAVVKRGDWYYNFWQDEKNVRGLWRRTTLQEYRKPEPKWEP
                     VLDLDQLSRDEKENWVWKGSSCLYPNYDRCLLTLSRGGADAAVVREFDIAAKAFVAGG
                     FQLKEAKGSAGWIDRNTLFVQTDFGPGSSTKSGYPRMVKEWKRGTPLESAKTVFEGKE
                     ADISVSAFQNEQKGYTTRQFVRRAVTFFTAEMFLREGNRLTRLDVPLDAQASYAQDML
                     WVRLKSAWTAGDRSYAQGSLIAINFERFMKGERNFDVLFEPTSRKSLAGWTLTKNYLI
                     LNELDNVKNRLHEVQYKNGKWNRRAITMPGLGTVGATAVDSDESDDYFLTVTDYLTPS
                     TLFLARAGGDTREQLKSLPAFFDASPYRVEQFDVASKDGERIPFFVVMNKNTKLDGRN
                     PTLLYGYGGFEVSQLPGYSATVGNAWLNNGGVYVVANIRGGGEFGPRWHQAGLKANRQ
                     KVFDDFIAVAEDLIARKITAPRHLAIYGGSNGGLLVGAVATQRPELFRAVLCTVPLLD
                     MKRYHKLLAGASWVAEYGNPDDPKEWEFISKYSPYQNVKAGVKYPKFLFVTSTRDDRV
                     HPGHARKMMARMMEQGHEVMYYENIEGGHAASANNQQLAYRTALQYSFLLNELK"
     gene            2293..2385
                     /locus_tag="PKV23_06455"
     tRNA            2293..2385
                     /locus_tag="PKV23_06455"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2327..2329,aa:Ser,seq:gct)
     gene            2477..2553
                     /locus_tag="PKV23_06460"
     tRNA            2477..2553
                     /locus_tag="PKV23_06460"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2511..2513,aa:Arg,seq:acg)
BASE COUNT          545 a          748 c          778 g          492 t
ORIGIN      
        1 catcgacgaa aaatacatgg agctggcggt ccgtgcattg catcaggcat tcgaactcga
       61 taaactcccc gcctgattaa agctatcacc cacaaggccg cgcgattccc gcgcggcctt
      121 gtcattttcc ggagccagcc atgtttggat acagagcggc aactatcgcc gcattcgctt
      181 ttggagctgc catgaccacc agcgcactcg cccaggaaga cccgtacctc tggctggaag
      241 aggtgatggg tgaccgcgcc atctcgtggg tcaaggaaca gaatgcgaag tcgcagaagt
      301 tgctcgaggc atcgccgcag tttgccgcca tccgaagcaa gtcgctggcg gtactgaatt
      361 cgcgtgcccg catcccggcc gtggtcaagc ggggcgactg gtactacaac ttctggcagg
      421 acgagaagaa cgtacgcggg ctgtggcgcc gcaccacgtt gcaggaatac cgcaagccag
      481 agccgaagtg ggaaccggtg ctcgaccttg atcagctttc tcgtgacgaa aaggaaaact
      541 gggtgtggaa gggttcgtcc tgcctgtacc cgaattacga tcgctgcctg ctgacgctgt
      601 cgcggggtgg cgccgacgcg gcggtggtgc gcgagttcga catcgcggcc aaggcttttg
      661 tcgccggcgg cttccagttg aaggaagcca agggcagtgc cggctggatt gatcgcaaca
      721 cgctgtttgt gcaaaccgat ttcggaccgg gatcaagtac caaatccggc tacccgcgca
      781 tggtcaagga atggaagcgt ggcacaccgc tggaaagcgc gaaaacggtg ttcgaaggca
      841 aggaggccga catcagcgtc agcgcctttc agaacgaaca gaagggatac accacgcgcc
      901 agtttgtccg tcgcgcggtg accttcttca ccgccgagat gttcctgcgc gaaggcaaca
      961 ggttgacgcg gctggacgtg ccgctcgatg cccaggccag ctatgcccag gatatgttgt
     1021 gggtgcgact caagtcggcc tggacagcgg gcgatcgttc ctatgcgcag ggttcgctga
     1081 tcgccatcaa tttcgagcgc ttcatgaagg gtgagcgcaa tttcgatgtg ctgttcgagc
     1141 caaccagccg caaatcactg gccggctgga ccctgacgaa gaattacctg atcctgaatg
     1201 agctcgataa cgtcaagaac cggctccacg aggtgcagta caagaacggc aaatggaatc
     1261 gccgcgccat caccatgccc gggctgggca cggtcggcgc caccgcagtt gattccgacg
     1321 agtcggatga ctacttcctg accgtcaccg actacctgac accgtcgacc ctgttcctgg
     1381 cccgcgccgg tggcgatacg cgtgaacagt tgaagtccct gccggcattc tttgatgcaa
     1441 gcccgtaccg ggtcgagcag ttcgatgtcg catccaagga cggcgagcgg attccattct
     1501 ttgtcgtgat gaacaagaac acaaaactgg atggccgcaa tccaacgctg ctgtacggct
     1561 acggcggctt cgaggtctcg cagttgcccg gctattcggc aacggtcggc aatgcctggc
     1621 tgaacaacgg cggcgtctac gttgtcgcca acatccgcgg cggcggcgag ttcggcccgc
     1681 gctggcacca ggccggactg aaagcgaacc ggcagaaggt gttcgatgat ttcatcgccg
     1741 tcgccgaaga cctgattgcc cgcaagatca ccgcgccgcg ccaccttgcc atctacggcg
     1801 gcagcaatgg cggcctgttg gtcggcgccg tggccacgca acgtccggag ttgttccgcg
     1861 ccgtgctctg caccgtaccg ctgcttgaca tgaagcgcta tcacaagctg ctggccggcg
     1921 cctcctgggt cgccgaatac ggcaatccgg atgaccccaa ggaatgggaa ttcatctcga
     1981 aatactcgcc ttaccagaat gtgaaggccg gcgtgaaata cccgaaattc ctgtttgtca
     2041 cctcgacccg tgacgaccgg gtgcatcccg gccacgcacg caagatgatg gcgcgcatga
     2101 tggagcaggg gcatgaggtg atgtattacg agaacatcga gggcggtcac gccgcctcgg
     2161 ccaataacca gcaactggca taccgtaccg cgctgcagta ttcgttcctg ttgaacgaac
     2221 tcaaatagcc gattgttttg acgcggggag ggtggaaacg gtacaatccg cccttcgcga
     2281 aattggcggt acggagacct ggccgagtgg tcgaaggcac tcccctgcta agggagcata
     2341 cgcccaaaag gtgtatcgag ggttcgaatc cctcggtctc cgccacgatt tcccgtcgaa
     2401 tttctcggga gatttgttcg tgaaatcagc gtgatagtga caatgcgcac gctgatcagt
     2461 tataattgtc gccgttgcgc ccgtagctca gttggataga gtacttggct acgaaccaag
     2521 gggtcgtggg ttcgaatcct gccgggcgcg ccagtttcaa gaa
//
LOCUS       DAORVN010000282         2554 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_39286_length_2554_cov_2.413766, whole genome shotgun sequence.
ACCESSION   DAORVN010000282 DAORVN010000000
VERSION     DAORVN010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2554
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39286_length_2554_cov_2.413766"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="PKV23_06465"
     CDS             <1..322
                     /locus_tag="PKV23_06465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85113.1"
                     /translation="WLNCSDGDRASFNVLLGNMNVIAVDTVEIVAGGKKWSTRGGDAS
                     LITKGQAFETADQILIDVTDSNVDAIVAQLRLFKASEGEDYVSAGTLRVAGVGAWAVT
                     CSGP"
     gene            322..1380
                     /gene="queA"
                     /locus_tag="PKV23_06470"
     CDS             322..1380
                     /gene="queA"
                     /locus_tag="PKV23_06470"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003586473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HQX85114.1"
                     /translation="MRVSDFDFELPPERIALRPANPRESAKLLVVGDGLGDSTVGDLP
                     SQLRAGDVLVFNNTKVIPAALTGVRLGRGEATPRIEALLHMRVDGSRWKAFAKPGKKL
                     QVGDRLRFGAEGRVCLLGDLDATVEAKGEAGEITLAFDFSGPVLDEAIEAVGQMPLPP
                     YIEGKRATDEQDATDYQTMFAQYPGAVAAPTAGLHFTPDLVKRLEAAGISSEFVTLHV
                     GAGTFLPVKAEDTRDHKMHAEWGEVSSGTVARLKAARQRGGRIVAVGTTSLRLLESSR
                     LEPFSGTTEIFITPGYQFCAADVLISNFHLPRSTLFMLVCAFAGTERMKAAYAHAIHN
                     DYRFYSYGDCCLLFPRAA"
     gene            1377..2495
                     /gene="tgt"
                     /locus_tag="PKV23_06475"
     CDS             1377..2495
                     /gene="tgt"
                     /locus_tag="PKV23_06475"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018494632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HQX85115.1"
                     /translation="MTEFSFSLHKADGAARLGTVRTLHGDIRTPAFMPVGTVGTVKGL
                     YLDQVKETRADVILGNTYHLMLRPGAEEVAKLGGLHKFIGWEGPILTDSGGFQVMSLA
                     KIRKITEQGAVFQSHIDGRRYELTPERAMEIQRLLGSDIQMQLDQCLEYPHTDKEAEK
                     AMQLSLRWAARSRKAFGIQPGRGNFGIVQGGVNEGLRRASAAGLRDIGFEGYAIGGLA
                     VGEGQKLMLQTIGFTAPELPVDKPRYLMGVGTPEDLLESVAHGIDMFDCVMPTRSGRH
                     GQAFTRFGKINLRNAKHADDPRPLDAESTCPALSKYSRAYIHHLMRSGELLGMMLLSW
                     ANVAYYQDLMQGIRGAIAEDRFEEFRALTKEQWKRGDA"
BASE COUNT          481 a          693 c          853 g          527 t
ORIGIN      
        1 atggctcaac tgctccgatg gcgacagggc ctcgttcaat gtcctgctcg gcaacatgaa
       61 cgtgatcgcc gtcgatactg ttgagatcgt agctggcgga aagaaatggt cgacgcgggg
      121 cggtgatgcc tcgctcatca ccaaggggca ggcattcgag accgccgatc aaattctaat
      181 cgacgtgacc gatagcaatg ttgatgcgat tgtcgcccag cttcgcctgt tcaaggcgag
      241 cgagggcgaa gactatgtct cggccggcac cctgcgggtg gcgggcgttg gcgcttgggc
      301 cgtgacctgc tccggtccct gatgcgcgtt tccgactttg attttgaatt gccgccggaa
      361 cgcattgcgc tgcggcccgc gaacccgcgc gagtccgcca aactgctggt ggtgggcgac
      421 ggacttggtg attccaccgt gggagatttg ccgtcgcaac ttcgggctgg cgatgtgctg
      481 gttttcaaca acacgaaagt cataccggct gccctgacgg gggtgcgcct tggtcgcggc
      541 gaggcgacgc caaggattga ggcgctgctg catatgcggg ttgatggctc acgctggaaa
      601 gcgtttgcca agccgggcaa gaaattgcaa gtaggcgacc gcttgcgttt tggggcggag
      661 ggccgggtgt gcctgttggg cgatcttgac gccacggtcg aagccaaggg cgaggcgggg
      721 gaaattaccc tggcatttga tttcagcggg ccggttttgg atgaggcgat tgaggcggtg
      781 gggcagatgc cgctgccgcc ctatatcgag ggcaagcggg cgaccgacga acaggatgcc
      841 acggattacc agacgatgtt tgcgcagtac ccgggggcgg tggcggcccc gacagcgggg
      901 ttgcacttca cgcctgatct tgtgaagagg ttggaagctg ctggaatttc tagtgaattt
      961 gtcacgctgc atgtgggggc cgggaccttt ttaccggtaa aggctgagga tacgcgcgac
     1021 cacaagatgc atgccgagtg gggggaagtt tcatctggaa cagtagctcg gctgaaggct
     1081 gcccggcagc ggggcgggcg catcgtggcg gttgggacca cttccttgcg cctgcttgag
     1141 agttcgagac tggagccctt cagcgggacg acggagatct tcattacccc gggctaccag
     1201 ttttgcgccg ccgatgtgct gatcagcaat tttcacctgc cgcgctcgac gctgttcatg
     1261 ctggtttgcg cctttgccgg gacagagcgg atgaaggctg cctatgccca tgccattcat
     1321 aatgattatc gtttctattc ctatggcgac tgctgcctgc tatttcctcg ggccgcatga
     1381 ctgaattttc cttctccctt cacaaggccg acggcgcggc gcggctgggc acggttcgca
     1441 ctctacacgg cgacattcgc accccggctt tcatgccggt gggcacggtg ggcacggtga
     1501 aagggttgta cctggaccag gtgaaagaaa cccgtgccga tgtcattctg ggtaacacct
     1561 atcatctgat gctgcggccc ggtgctgagg aggtggcaaa gctcggcggc ttgcacaagt
     1621 tcataggctg ggagggacca atcctgaccg acagcggcgg cttccaggtc atgtcactgg
     1681 caaagatccg caaaatcacg gagcaggggg ctgttttcca atcgcatatt gacgggcgac
     1741 gctatgaact cacgccagag cgggccatgg agatccagcg cctgcttggc tccgacatcc
     1801 aaatgcagct cgaccaatgc ctggagtatc cgcacacgga taaagaggcg gaaaaagcca
     1861 tgcagctttc gctgcgctgg gcggcgcgct cacgcaaggc tttcgggatt caaccagggc
     1921 gcggcaattt cggcatcgtg cagggtggcg tgaacgaagg tttgcgccgg gccagtgcgg
     1981 cggggcttcg ggatatcggt ttcgaaggat atgcaatcgg cggactggcg gtgggcgaag
     2041 gccagaagct gatgctgcag acgatcgggt tcacggcgcc ggagctgcct gtggacaagc
     2101 cgcgctatct catgggagtt ggcacgccgg aagatttgct cgagtccgtg gcgcatggga
     2161 ttgacatgtt cgactgcgtg atgccgaccc gctcggggcg gcatggacag gctttcacgc
     2221 ggtttggaaa gatcaatctg cgcaatgcca aacatgcgga tgatccgcgc ccgctcgatg
     2281 ctgaaagcac ttgtcctgcg ttgtccaagt attcgcgggc ttacatccac cacctgatgc
     2341 gatccggcga attgctcggc atgatgctgc tgtcctgggc caatgttgcc tattatcagg
     2401 acctcatgca gggcattcgc ggggccattg ccgaagaccg cttcgaagaa ttcagggcgc
     2461 tcacaaaaga gcagtggaag cgcggcgacg cctaaccggg ataccagatc ttgcgcgggt
     2521 cattggcggc gcgcttgtag tcccaggcgg cgtc
//
LOCUS       DAORVN010000283         2546 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_39497_length_2546_cov_2.747491, whole genome shotgun sequence.
ACCESSION   DAORVN010000283 DAORVN010000000
VERSION     DAORVN010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2546
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39497_length_2546_cov_2.747491"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1042)
                     /locus_tag="PKV23_06480"
     CDS             complement(<1..1042)
                     /locus_tag="PKV23_06480"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HQX85116.1"
                     /translation="MLTIIDRYVLRQVAKPLATAMAIGLLMLLAERLVRLLDTTLGKK
                     NSFGVVFELLAYLVPHYLGTAIPAALFLGLLFGFNKLSKDSEIDAIMATGTGLHRLTR
                     PVILLSLALGALSLFIVGWLQPQTRYAYRSVLFDVKNVEVFYLAEEGVFMQAGTRTFI
                     LDKLNRSSNTFDHVFLFDYRGPSGLDTLTASSGMLIPLDGDRRPVLRLDNGHRLKLDR
                     WPSLDGKAEIPQATVVEFATTDTPLGRVSDSIFRPRGEDERELTLPELYTQLDTPPKG
                     SSQFHAGRTAQENCQCRHFFHAAPARRALCDRSPAQSARLSFRLGHGHRCGFPRNHRA
                     GGPDHQDERDHPL"
     gene            complement(1047..1484)
                     /locus_tag="PKV23_06485"
     CDS             complement(1047..1484)
                     /locus_tag="PKV23_06485"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HQX85117.1"
                     /translation="MNATMQKFGYPGSLVKEHHHWCVLLRPQQVTLGSLVLAAKSDAT
                     AFSDLPPTAFAELSIVVTDIEQSLRRFNPYDRINYLMLMMVDPHVHMHVLPRYARPQF
                     FDATEFPDAGWPGPPDLKSAPVLTDKLRKNLLWTLQLRMSGES"
     gene            complement(1511..2416)
                     /locus_tag="PKV23_06490"
     CDS             complement(1511..2416)
                     /locus_tag="PKV23_06490"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HQX85118.1"
                     /translation="MALTLAHLSDVPLGPLPPGAAWQNFALKRLVGAVNWNWRRHKQH
                     DRAISSSLVADIVAASPDHVAFTGDLLNLSAYAEFPRAARWLQSFGDPAWISYVPGNH
                     DAYVPVPWKYGLAHLAPYMTGDMAAPGSQASFPYVRLRRNLALIGLNSGVPQSLTRAA
                     GALGPKQLEALPGILHDLKAKGYYRAVMIHHPPLPGLAPPRKALRDAAQLRDILASEG
                     AELVLHGHNHQQMLNVLAGRDGDIPVIGVPSASMNETGHAEPAAWNLYEISRNQGTWV
                     THVSIRAWDPKSRRMLAKNHFTLPS"
BASE COUNT          554 a          676 c          820 g          496 t
ORIGIN      
        1 ccagggggtg atcccgctca tcctggtggt cagggccccc tgctcgatga tttcgtggaa
       61 agccacaacg atgaccatgg ccaagccgaa acgatagccg cgctgactgc gctggcgacc
      121 gatcgcaaag ggcacggcga gcaggggcag catgaaaaaa gtgacgacat tgacaattct
      181 cttgtgcagt tctgccagca tggaattgcg aggaaccttt gggcggcgtg tcaagctggg
      241 tgtaaagctc aggcagggtg agttcgcgct catcttcgcc gcgcggacgg aatatgctgt
      301 cggacactct tcctagcggg gtatcagtgg tggcaaattc gactacggta gcctgcggaa
      361 tctcggcctt gccgtcaagg ctgggccagc ggtcaagttt cagccgatgg ccgttgtcca
      421 ggcgcagaac cgggcggcgg tcgccgtcaa gagggatcag cattcccgat gaagcagtaa
      481 gtgtatccag ccccgatggg ccacggtaat cgaagagaaa aacatgatcg aaggtgttgc
      541 ttgagcgatt cagtttgtca agaatgaagg tgcgggtgcc tgcctgcatg aagacgcctt
      601 cctcggcgag atagaaaacc tccacattct tcacgtcaaa caggaccgag cggtaggcat
      661 agcgggtctg cggctggagc cagccaacaa taaaaagcga aagagcccca agggccagcg
      721 aaagcaagat gaccggccgg gtcagccggt gcaggccggt tccggtggcc attatggcgt
      781 caatttcgct gtcctttgac agcttgttga agccaaacag aaggccgagg aagagggccg
      841 ccggaatggc ggttccgaga taatgcggca cgagataagc gagcaattcg aagaccacgc
      901 caaaggaatt cttcttgccg agtgttgtat cgagcaggcg cacgagcctt tcggccagga
      961 gcatgagcaa accgatcgcc atggctgttg ccaatggctt tgccacctgc ctgaggacat
     1021 agcggtcgat aatggtcagc acaggcttat gattctccag acatccgcag ctgcagggtc
     1081 cataacaggt ttttgcgcag cttgtcagtc agcactgggg cggatttgag gtcgggaggg
     1141 ccgggccagc cggcatcggg aaactccgtc gcatcgaaga actgcggcct ggcatagcgc
     1201 ggcagcacat gcatgtgcac atgcggatca accatcatca gcatgagata attgatcctg
     1261 tcatagggat tgaacctgcg caggctttgc tcgatgtcgg taaccacgat tgaaagttcg
     1321 gcgaaagcgg tgggcggcag atcggagaac gccgtcgcat cggattttgc ggcaagcacc
     1381 aggctgccaa gcgtcacctg ttgcgggcgc agcaggacgc accagtggtg gtgttcctta
     1441 accaggctgc cgggatagcc aaatttctgc atcgtggcgt tcatggccgg caacttaccc
     1501 cgaagcgggc tcaggaagga agggtgaagt ggtttttggc cagcatccgg cgggatttgg
     1561 gatcccatgc ccgtattgaa acgtgggtca cccaagttcc ctgattgcgg ctgatttcat
     1621 agaggttcca tgcggcgggt tcagcgtggc ccgtttcatt catggacgcg gagggcacgc
     1681 caataaccgg aatatcgccg tcacggcctg caagaacatt gagcatttgc tggtggttgt
     1741 gtccatgcag caccagttcc gccccttcgc ttgccaaaat gtcgcgcaat tgagcggcat
     1801 cacggagggc ttttcgcggc ggcgcgaggc ccggcagggg aggatgatga atcatgacgg
     1861 cccggtaata gcccttggcc ttcaagtcat gcaatattcc cggcagcgct tcgagctgct
     1921 tggggccgag ggcgcccgcc gcccgggtca gggattgggg aacgcccgaa ttcaagccaa
     1981 tcagcgccag attgcggcgc agccgcacat aggggaatga cgcctgcgaa ccgggtgctg
     2041 ccatgtctcc ggtcatgtaa ggggcgagat gggcaaggcc gtatttccac ggcacgggca
     2101 cgtaggcgtc gtggtttcca ggcacatagg aaatccaggc gggatcgcca aagctctgca
     2161 gccagcgcgc ggcgcggggg aattcagcgt aggccgatag gttcagcagg tcgccagtga
     2221 aggcgacgtg atcagggctg gctgccacaa tgtcggcgac aaggctgctg gaaatggcac
     2281 ggtcgtgctg tttgtgtctt ctccaattcc agttcaccgc accgaccagg cgtttcaggg
     2341 cgaaattctg ccaggcggcg ccgggcggca atggccccaa agggacatcc gacagatgag
     2401 cgagcgttag ggccaaatct tagcttccgt tgacttccgg ggaatcgggt gcgaatgagc
     2461 agtccgtact tacccttctg ttagacgtgg attcaagcat tgtgcgtata gttattcttg
     2521 cagcaggcca gggcaaacgg cttttg
//
LOCUS       DAORVN010000284         2542 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_39589_length_2542_cov_3.518697, whole genome shotgun sequence.
ACCESSION   DAORVN010000284 DAORVN010000000
VERSION     DAORVN010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2542)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2542
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39589_length_2542_cov_3.518697"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            3..1361
                     /gene="xseA"
                     /locus_tag="PKV23_06495"
     CDS             3..1361
                     /gene="xseA"
                     /locus_tag="PKV23_06495"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006022215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HQX85119.1"
                     /translation="MEQPLPNVGEYSVTEISHALKKTLEETFGHVRVRGEISGFRGPH
                     SSGHCYFSIKDEGAKLDAVIWRGNFSKLRARMQEGLEVVATGKITSYPGKSNYQIVIE
                     NIEPAGVGALMALLEERRRKLAAEGLFDADRKKPLPYLPKLIGVVTSPTGAVIRDILH
                     RLSDRFPRHVLVWPVRVQGETSAAEVAAAIIGFNAMANPPDLIIVARGGGSLEDLWGF
                     NEEIVVRAVAGSKIPIISAVGHETDTTLIDLVSDHRAPTPTAAAERAVPVRADLLAEL
                     ASLNARQARSVARYFDERRTRLRSAARALPKPDEFLALVRQRFDTVGARLPQALRANV
                     QHHRVDLATISGRLSVRPIVQNQQEMKRRLAEADLRGNRAMSRVLQSLRDRLSAEAKL
                     FAALNYTSVLARGFALVLDTRGQPVKQAAAVTPGQTLKIQFSDDTLTVQAAKKTAQGE
                     LF"
     gene            1375..1590
                     /locus_tag="PKV23_06500"
     CDS             1375..1590
                     /locus_tag="PKV23_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2093 domain-containing protein"
                     /protein_id="HQX85120.1"
                     /translation="MNYLDPNLPKQREAKLKYLNADYQVLREGDYVRCSVSGHPIKLE
                     NLRYWDVERQMAFGSAEISFSEFLARL"
     gene            1726..>2542
                     /locus_tag="PKV23_06505"
     CDS             1726..>2542
                     /locus_tag="PKV23_06505"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008139623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HQX85121.1"
                     /translation="MLTNWTPDSWRKFPVVQVPDYPDTQKLSVVEKRLAGFPPLVFAG
                     EARKLKRKLARVAEGRAFLLQGGDCAESFAEHSADNIRDFFRVFLQMSVVMTFAGGQP
                     VVKVGRIAGQFAKPRSSPTEVIDGVEYPIYRGDIINDSEVTVEGRKPDPERQIMAYRQ
                     SAATLNLLRAFAQGGYANLEHVHKWTLGTLKDSPAFERYQELADRITEALGFMRACGI
                     NADTAPQLRQTDFFTSHEALLLGFEQAMTRVDSTSGDWYATSGHMLWIGDRTRQ"
BASE COUNT          494 a          834 c          728 g          486 t
ORIGIN      
        1 ttatagagca gccgctgccc aatgtgggcg agtattcggt caccgagatt tcccatgccc
       61 tcaagaaaac cctcgaggaa accttcggcc atgtgcgggt gcgcggcgaa atctcaggct
      121 ttcgcggccc ccattcctcc ggccactgct atttctcgat caaggatgaa ggcgccaagc
      181 tcgatgccgt gatctggcgc ggcaattttt ccaagctccg cgccaggatg caggaagggc
      241 tggaagtcgt cgccaccggc aagatcacct cctatcccgg caagtccaat taccagatcg
      301 tcatcgaaaa tatcgagccc gccggcgtcg gcgcgctcat ggcgctgctt gaggagcgta
      361 ggcgcaagct cgccgccgaa ggcctgtttg atgccgaccg caagaagccg ctgccatatt
      421 tgccaaagtt gatcggcgtg gtgacttcgc ccaccggcgc cgtgatccgc gatatcctcc
      481 accgcctgag tgaccgcttc ccgcgccatg tccttgtctg gcccgtgcgc gtgcagggcg
      541 aaacctcggc ggcggaagtg gccgctgcca tcatcggctt caacgccatg gccaatccac
      601 ctgatctgat catcgtggcg cgtggcggcg gctcgcttga agacctctgg ggtttcaacg
      661 aggaaattgt cgtccgcgcc gtggcgggca gcaaaatacc gattatctcc gccgtgggcc
      721 acgaaaccga tacgacgctg atcgatctcg tctccgatca ccgggcgccc acaccaaccg
      781 ccgccgccga gcgtgccgtt ccggtgcgcg ccgatctgct ggcagagctg gcctccctca
      841 atgcaaggca ggcccggagt gtggcccgct atttcgatga acgccgcacc aggctgcgct
      901 ccgccgcccg cgccctgcca aagcccgatg aattcctcgc cctcgtgcgc cagcgctttg
      961 acacggtggg cgcaaggctg ccccaggcac tgcgcgccaa tgtgcagcat caccgcgtgg
     1021 acctcgcgac aatttccggc aggctttccg tgcgccccat tgtccagaac cagcaggaaa
     1081 tgaagcgccg cttggctgag gcagatctgc gcggcaatcg cgccatgtcc cgcgttctgc
     1141 aatccctcag ggaccggctt tccgccgaag ccaaactctt cgccgcgctg aactacacct
     1201 cggttctggc ccgcggcttt gccctggtcc tcgacaccag gggccagccg gtcaaacagg
     1261 ccgccgcggt cactcccggt caaaccttga aaattcagtt cagtgatgac acattgacgg
     1321 ttcaggccgc caaaaagact gcccagggcg aactctttta aattaggaaa agcgatgaat
     1381 tatctcgacc ccaatttgcc caagcagcgc gaggcaaagc tgaagtactt gaacgccgat
     1441 tatcaggtcc tgcgcgaggg cgattatgtc cgctgcagcg tgtcgggcca ccccataaag
     1501 ctggaaaatc tgcgctattg ggacgtcgaa cggcagatgg cctttggctc ggcggagatc
     1561 tcctttagcg agtttctggc caggctctaa gccacccctt ccggaaatct ctgcattatc
     1621 agtgaaatag gggaattaac cgtcggttgc cccgcctgcg gtaagactgg aatagctggg
     1681 aaaccgggtg tataagccca ttcctttgta tctggagcga agactatgtt gaccaattgg
     1741 acacctgaca gctggcggaa attccccgtt gtgcaggtgc cggactaccc ggacacacaa
     1801 aaacttagcg ttgtggaaaa gcgccttgcc ggttttccgc cgctggtttt tgccggcgag
     1861 gcgcgcaagc tgaagcgcaa actggcgcgc gtcgccgagg gcagggcttt cctgctccag
     1921 ggcggcgatt gcgccgaaag cttcgccgag cattccgccg acaacatccg cgatttcttc
     1981 cgcgtcttcc tccagatgtc ggtggttatg acctttgccg gcggccagcc cgtggtgaag
     2041 gtgggccgca ttgccgggca attcgccaag ccgcgctctt cgccaaccga ggtgatcgat
     2101 ggcgtcgaat acccgatcta tcgcggcgac atcatcaatg attcggaagt gacggttgaa
     2161 ggccgcaagc ccgatcccga acgccagatc atggcctacc gccagtcggc ggcaaccctc
     2221 aatctgctcc gcgcctttgc ccagggcggc tatgccaacc tggagcatgt ccacaaatgg
     2281 accctcggca ccctgaagga ttcgcccgct ttcgagcgtt accaggaatt ggctgaccgc
     2341 atcaccgaag ccctgggctt catgcgcgcc tgcggcatca atgccgatac cgccccgcaa
     2401 ctgcgccaga cggatttctt caccagccat gaagccctgc tgctcggctt cgagcaggcc
     2461 atgacccgcg tcgattccac cagcggcgac tggtatgcaa cctcgggcca catgctgtgg
     2521 atcggcgacc gcacccgcca gc
//
LOCUS       DAORVN010000285         2535 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_39789_length_2535_cov_2.769355, whole genome shotgun sequence.
ACCESSION   DAORVN010000285 DAORVN010000000
VERSION     DAORVN010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2535
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39789_length_2535_cov_2.769355"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..965)
                     /gene="lptF"
                     /locus_tag="PKV23_06510"
     CDS             complement(<1..965)
                     /gene="lptF"
                     /locus_tag="PKV23_06510"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HQX85122.1"
                     /translation="MIFQRAAIREFTHTAAGVFVALFAILMTTQLIRLLGDAAGGKLA
                     SEAVIALLGFRAISYLPVLLSLTVFIAVLMTLSRWYRDSEMVVWLASGISLTSWVVPV
                     LKFSGPPAVVIALLSLLLAPWANWKSETYRQTMNQRDDVARVSPGAFNESSSADRVFF
                     VESVAGEDGRVKNVFISSIQHGRLGVMATAQGHTETMGNGDRFLVLDRGRRYEGTAGT
                     AEYRVMEFDRYFVRIETKEARGFEDSPRTKPLWELLRDQQPANLAELLWRIGLPLAAL
                     NLAVLAIPLAFVNPRAGRANNLIFALLAYMIYSNLLSVSQAWVAQG"
     gene            1087..>2535
                     /locus_tag="PKV23_06515"
     CDS             1087..>2535
                     /locus_tag="PKV23_06515"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF002074.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HQX85123.1"
                     /translation="MEFSIKSGSPEKQRSACVVVGVFETRKLSAAAETIDKASLQFLS
                     DILRRGDMEGKAGTSLLLHGVSGIEADRVLLIGLGKEREFREKEYRTAVATAVRQLNE
                     TGGFDGTVFLTELAVRKRDVSWKIRQAALVAQEALYRFDRLKSKKEAVRRPLRKLTFF
                     VARRNELAAAELALAQGLAMGAGVELAKDLGNLPGNICTPGYLADQARQLAKLYGLGV
                     DVLDRADMEKLGMNTLLSVARGSHEPPKFIVLRHSGGPSGAKPVVLVGKGITFDSGGI
                     SLKPGADMDEMKYDMCGAASVLGTLKSIAMLELPLNVVGVIPSTENMPGGAATKPGDV
                     VTSMSGQTVEILNTDAEGRLILCDALTYVERFEPQCVVDIATLTGACVIALGHVATGL
                     LANNEALAKELLDAGQEAYDRAWQMPLWDDYQEQLKSNFADMANIGGRPAGTITAACF
                     LARYTEKYQWAHLDIAGTAWRSGKEKGATGRPV"
BASE COUNT          589 a          736 c          736 g          474 t
ORIGIN      
        1 ccttgcgcaa cccatgcctg gctcacactc agcaggttgc tgtagatcat gtaggcaagc
       61 agcgcaaaaa tgaggttgtt ggcgcgtccc gcgcgtggat tgacgaacgc cagaggtatt
      121 gcaagcacgg ccaggtttaa cgccgcaagc ggcaacccga tgcgccagag caactcggcc
      181 agattggccg gttgctggtc acgcaacaat tcccatagcg gtttggtacg gggagaatcc
      241 tcgaaacccc gggcctcctt ggtctcgatc cgaacaaagt atcgatcgaa ttccattacc
      301 cgatactccg ccgttcccgc tgtgccttca tagcgacggc cgcgatcaag aaccagaaat
      361 ctgtcgccgt tgcccattgt ctctgtatgg ccttgtgcag ttgccatgac acctagtcgg
      421 ccatgctgga tcgaactgat gaacacgttc tttactcttc catcttcgcc ggccacgctt
      481 tccacgaaaa atacgcgatc ggccgaactg gattcattga atgccccggg tgacacccgc
      541 gccacgtcgt cccgctgatt catggtctgg cgataggttt cacttttcca atttgcccaa
      601 ggcgcaagca gcagggaaag tagagcgatc accaccgctg gcggccccga gaacttcaga
      661 acgggaacga cccaggacgt cagggagatc cccgatgcca gccaaacaac catttccgaa
      721 tcgcgatacc agcgcgacaa ggtcatgagc acggcaatga agaccgtcag ggacagaagc
      781 accggcaaat agctgatcgc ccgaaagcca agcaatgcga ttacggcttc tgaagccagt
      841 ttgccgcccg cagcatcacc cagcaggcgg atcaactggg tcgtcatcag gatcgcgaat
      901 agtgcgacga aaactcccgc agccgtgtgc gtaaactcgc ggatagcagc acgctgaaaa
      961 atcattgctt agcagaacgt ccgctttgac atttggagaa aacttgagga ataatggcca
     1021 accgcaacga aatcgcccga aacatgatgc tttcgtggat ttccagtcgc cggaaaggag
     1081 taagttttgg aatttagcat aaaaagcgga agcccggaaa agcagcgcag cgcctgcgtc
     1141 gtggtcggcg ttttcgagac gcgaaagctt tctgcggccg ccgaaacaat cgacaaggcc
     1201 tccctgcaat ttctcagcga tatcctccgt cgcggcgaca tggaaggcaa agctggaacc
     1261 agcctcttgc tccatggtgt ttccggcatc gaagcagacc gggtcctatt gattggactg
     1321 ggcaaggaaa gggagttccg tgaaaaggaa taccgtaccg cggtagcgac tgcagtccgt
     1381 cagctcaatg aaaccggcgg cttcgacggc accgtgttcc tgacggaact cgccgttagg
     1441 aaacgtgacg tgtcctggaa gatccggcaa gccgcgctgg tggcgcagga ggccttgtat
     1501 cgattcgacc gactcaagtc gaaaaaggag gcggtacgcc gtccattgcg caaattgact
     1561 ttcttcgtcg cacgccgcaa cgaactcgcc gccgccgaac tggctctcgc acaaggtctg
     1621 gcgatgggtg ccggcgttga actggcgaag gacctcggca atcttccagg caatatctgt
     1681 acgcctggct atctcgccga ccaggctcgg caactcgcaa aactttatgg attgggcgtc
     1741 gacgtccttg accgcgccga catggagaag ctgggcatga acaccctgct ttcggtggca
     1801 cggggatcgc acgaaccacc gaaattcatc gtcctgcgcc actccggagg cccgagcggc
     1861 gcgaagccgg tggttcttgt cggcaagggc atcacattcg attccggcgg catttcgctc
     1921 aagcccggcg ccgatatgga tgaaatgaaa tacgacatgt gcggcgccgc cagcgtcctg
     1981 ggaaccctca agtcaattgc aatgttggaa cttccgctaa atgtcgtcgg cgtgattcct
     2041 tcgacggaga acatgccggg tggtgcggcg acgaaaccgg gcgatgtagt tacctcgatg
     2101 tcgggccaga cagtcgagat actcaacacc gatgccgagg gacgactgat actttgtgac
     2161 gcgctgacct acgtcgagcg cttcgaaccg caatgcgtcg tggatatcgc gacgctgacc
     2221 ggagcctgcg tgattgcact gggccacgtc gcgacgggat tgctggccaa caacgaagcc
     2281 ttggcgaagg aattgctgga tgcgggccag gaggcctacg acagggcttg gcagatgcca
     2341 ctttgggacg actaccagga acagctgaag agcaacttcg ccgacatggc gaatatcggc
     2401 ggacgaccgg ccgggacaat tacggcggcg tgcttcctcg cgcgatacac ggaaaaatat
     2461 caatgggcgc acctcgacat cgccggcacc gcctggcgct ccggcaagga gaagggagcc
     2521 accgggcgtc cagtt
//
LOCUS       DAORVN010000286         2518 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_40276_length_2518_cov_3.743402, whole genome shotgun sequence.
ACCESSION   DAORVN010000286 DAORVN010000000
VERSION     DAORVN010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2518
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40276_length_2518_cov_3.743402"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..343)
                     /locus_tag="PKV23_06520"
     CDS             complement(<1..343)
                     /locus_tag="PKV23_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase"
                     /protein_id="HQX85124.1"
                     /translation="MIKIYVPKDSAARAVGADAVADAIVAEAASRKIDAKVIRNGSRG
                     LLWLEPFVEVETPKGRVAFAPVETGDVKSLFDSKFHEGGTHRLSLGLTEELPYLKNQE
                     RLTFARCGVTDP"
     gene            complement(340..831)
                     /locus_tag="PKV23_06525"
     CDS             complement(340..831)
                     /locus_tag="PKV23_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018181561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit gamma"
                     /protein_id="HQX85125.1"
                     /translation="MSGKHTLIDVHVTKPDAASLIAARYDNKPDALLEILHDLQHEVG
                     HIPEASLPVLAKALNLSRAEVHGVVSFYHEFRRKPAGRHVVKVCRAEACQSMKGNELA
                     DAAQKSLKVKFGETTSDGAITLEAVYCLGLCATSPALLVDERPMGRMTPQKFDALVKE
                     LAS"
     gene            complement(900..1103)
                     /locus_tag="PKV23_06530"
     CDS             complement(900..1103)
                     /locus_tag="PKV23_06530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85126.1"
                     /translation="MRDPINLGYHYCKSTERNVSLEQSEEQCRERHNCADGPCPLESE
                     FNEGRFSRAITLLLSGLNRDPDS"
     gene            complement(1185..2315)
                     /locus_tag="PKV23_06535"
     CDS             complement(1185..2315)
                     /locus_tag="PKV23_06535"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85127.1"
                     /translation="MTAKFLLMLFLMSLHLPAVSWVGKHEEGYVAFQKGDYAKALEKW
                     KPLAENGHAQAQLSLGTMYDKGQGVAQDYAEAAKWYRKAADQDVGMAQHNLGRMYQAG
                     RGVSQDFAEAAKWYLKAADQGVLEAQENLAVMYERGQGVPQDPVQAVRLYRRAAELGN
                     ATAQYSLGVSYENGLGVAPDAVEAAKWFALAAAQGNPKAQYSLGVSYDLGKGLPQNDA
                     EAFKWYREAVRQGYAPAQYKFGALYASGRGVEKSPAQALRWYLRSAKQGYAPAQHEVA
                     GLYGSGQGVKQDLAEAARWYRRAAQQGHVPAQYSLGLMYYNGQGVRQDNVQAHLWFSL
                     AAEGGEMEAVSQRDVVTKLMSPEDIAKAEQLASKWRAARPAP"
BASE COUNT          454 a          814 c          702 g          548 t
ORIGIN      
        1 gcggatcggt cacgccgcag cgcgcgaagg tcaggcgctc ctggtttttg agataaggaa
       61 gctcttcggt caggccgaga gacagcctgt gcgtgccgcc ctcatggaac ttggaatcga
      121 acaggctctt cacatcacca gtttccaccg gagcgaaggc cacgcggccc tttggcgtct
      181 cgacttcgac aaagggctcg agccacaaaa gcccgcgcga accgttgcgg atgactttcg
      241 cgtcaatctt ccgggaagcg gcttccgcca caatggcatc ggccaccgca tcagcaccaa
      301 cggcgcgggc ggcggaatcc ttcggcacat aaattttgat catgaagcca gttccttgac
      361 cagcgcgtca aacttttgcg gcgtcatgcg gcccattggc cgctcgtcca ccagcagggc
      421 aggagaggtg gcgcacagcc ccaggcaata taccgcctcc agagtaatgg ctccatccga
      481 tgtggtctcg ccgaatttca ctttcaagct tttctgggcg gcgtcggcca gctcattgcc
      541 cttcatcgat tggcaggcct ccgcccggca gaccttcacc acatggcggc ccgcaggttt
      601 gcgccggaat tcatggtaaa agctcaccac gccatgcact tcggcgcggg aaaggttcag
      661 cgcctttgcc agaaccggaa gcgaggcttc aggaatatgg ccgacctcat gctgcagatc
      721 atggaggatt tcgagcaatg cgtcaggctt gttgtcataa cgcgcggcta tgagactggc
      781 agcatcaggt tttgtgacgt gaacatctat gagggtgtgt tttccggaca tgcgccatta
      841 tggcagatac ccagggttta gccaattgcg ccagcctatg ggcctatagg ccaggccgat
      901 caggaatccg gatcccggtt caacccagac aaaagcaggg taatcgccct ggaaaaccgg
      961 ccctcgttga actcagactc gagcgggcag ggaccatcgg cgcaattgtg gcgctcgcgg
     1021 cattgctcct cgctttgctc cagcgatacg ttccgctcgg tactcttgca gtaatgatag
     1081 cccaggttga tcggatcgcg catgagtcct gcctccgccc gccaatgggc tccgggtgcc
     1141 ggaattcgtc attgatctgg atcaagatat cgacttcgcc ttgcctaggg tgcagggcgg
     1201 gcggcccgcc acttgctggc gagctgttcc gctttggcta tgtcctcggg actcatcagt
     1261 tttgtcacca catcacgttg gctgaccgct tccatttcgc cgccctcagc cgccaggctg
     1321 aaccacagat gcgcctggac attatcctgg cgcacgccct ggccattata atacatcaac
     1381 cccaggctat attgcgccgg tacatggccc tgctgggccg ccctgcgata ccacctggcg
     1441 gcttctgcca aatcctgctt tacgccctgg ccgctgccgt acaacccggc aacctcatgc
     1501 tgggctggcg catatccctg cttggctgac cgcagatacc atctgagcgc ctgggctggg
     1561 cttttttcaa cgcctctgcc actggcataa agtgcaccga atttgtattg cgctggcgca
     1621 tatccctgcc ggacggcttc ccggtaccat ttgaaggctt ccgcatcgtt ttgcggcagg
     1681 ccctttccaa ggtcgtaact gacgccgaga ctgtactggg ccttgggatt tccctgcgcc
     1741 gcggcaaggg caaaccactt tgcggcttca acggcgtccg gagccacgcc caggccattt
     1801 tcataactga cgccgaggct gtattgcgcc gtggcgttcc caagttcggc ggctctacgg
     1861 tacagtctga ccgcctgtac ggggtcctgc ggcacgcctt gccctctctc gtacatcacc
     1921 gcgagatttt cctgggcttc aagcacgccc tggtcggcgg ccttgagata ccattttgcg
     1981 gcttcggcaa aatcctgtga tacgccccgg ccggcctggt acattcttcc cagattgtgc
     2041 tgtgccatgc caacgtcctg gtcggcggcc ttgcgatacc acttcgccgc ctccgcatag
     2101 tcctgcgcca cgccctggcc cttgtcgtac atcgtgccga gcgaaagctg cgcctgggca
     2161 tgcccgttct cggccagcgg cttccacttc tccagggcct tcgcatagtc gcccttttga
     2221 aacgcgacat agccttcctc gtgcttgccc acccaggaaa ccgccggcag gtgaagcgac
     2281 atcaggaaca gcatcaaaag gaatttggcg gtcatggcag tcagttgatt ccttggcgct
     2341 gtcctttgct tttcaagtca tgccgcaagg cggctcttct ccttggcgaa ggggctgaag
     2401 ccaagccact cctgcatggc gccggcgatg gccctgtcgc cctggatgtc gagctcgcct
     2461 gccgccacct ctttcttcac cgtggtcacg cccatccaga ctgccgtcat ggagcgca
//
LOCUS       DAORVN010000287         2516 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_40342_length_2516_cov_3.007720, whole genome shotgun sequence.
ACCESSION   DAORVN010000287 DAORVN010000000
VERSION     DAORVN010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2516
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40342_length_2516_cov_3.007720"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(54..785)
                     /gene="hisA"
                     /locus_tag="PKV23_06540"
     CDS             complement(54..785)
                     /gene="hisA"
                     /locus_tag="PKV23_06540"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HQX85128.1"
                     /translation="MILFPAIDLKDGQCVRLKLGDMEQATIFNDNPAAQAKSFEDQGF
                     AWLHLVDLNGAFEGKPVNARAVEEILKAISIPVQLGGGIRDLATIERWLSRGVTRVIL
                     GTIALRNPAIVREACKEFPGEIVVGIDAKAGHVAVEGWGESSSLTAIELGKRFEDVGV
                     TAIVFTDIDRDGILKGINFEKTLELANSVNIPVIASGGLASIRDVERRLQPDCAILEG
                     AISGRAIYDGRLDAKAALKLIHEAA"
     gene            complement(782..1423)
                     /gene="hisH"
                     /locus_tag="PKV23_06545"
     CDS             complement(782..1423)
                     /gene="hisH"
                     /locus_tag="PKV23_06545"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018409004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HQX85129.1"
                     /translation="MKVAIIDYGSGNLHSALKAFERAAGASGQIIVTADPDVVRKAER
                     IVLPGVGAFKDCYEGLMAVPGMRDTLQEQVIARGKPFLGICVGMQLMATRGLEHDITP
                     GLGWIAGDVKLITPADRTLKIPHMGWNTLDVKNPHALLDGIALGENGLHAYFVHSYQL
                     EATLPEDVVATTDYAGPVTAMVGYDNVAGTQFHPEKSQVLGLALIANFLRWKP"
     gene            complement(1429..1851)
                     /locus_tag="PKV23_06550"
     CDS             complement(1429..1851)
                     /locus_tag="PKV23_06550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HQX85130.1"
                     /translation="MTTYSIYARPDDRGAADAIFLPDGFSWRAFVFTWVWALWNRMWI
                     VALLALSVMLIASALPTVPQFLLSLAVSLVMGLHGNDLLGWSLTRRKYAEIALSNGAS
                     MEEAELRFYAGDADVPALHAPTVLPAAHDVLGLFGARS"
     gene            complement(1851..2444)
                     /gene="hisB"
                     /locus_tag="PKV23_06555"
     CDS             complement(1851..2444)
                     /gene="hisB"
                     /locus_tag="PKV23_06555"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009467189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HQX85131.1"
                     /translation="MRKASLDRKTTETQIAVSINLDGTGAYDVKSGVGFLDHMLELLA
                     RHSLIDIKVRAKGDTHIDFHHTTEDTGIALGQAFAKALGDRKGIRRYADCFLPMDEAL
                     TRAAIDVSGRPFLIWKVEFARPKIGEFDTELVREWFQAFAMNAGVTLHVQTLYGDNCH
                     HIAESCFKALARVLRGAIEIDPRQKDRIPSTKGSLKG"
BASE COUNT          491 a          740 c          793 g          492 t
ORIGIN      
        1 gaccacgcgg ccgtccttga cgtcgaggca gggaatgatg cgggctttga gcattatgca
       61 gcctcgtgga tgagcttgag cgcggccttg gcatcaagcc tgccgtcata aatggcgcgg
      121 ccggagattg cgccttcgag aatggcgcaa tccggttgca gccgtcgctc cacatccctg
      181 atggaggcta ggccgcccga cgcgatgacc ggaatgttca cgctgttggc gagttccagg
      241 gtcttttcga aatttatgcc cttgaggatg ccgtcgcggt cgatgtcggt gaagacgatt
      301 gccgtgacgc ccacatcttc gaagcgtttg ccgagctcga tagccgtgag ggacgagctc
      361 tcgccccagc cttcaaccgc gacatggccc gccttggcat caatgccgac aacaatttcg
      421 cccggaaact ccttgcaggc ttcgcgcacg atggcgggat tccgaagtgc tatggttccg
      481 aggatgacac gggtcacgcc gcgcgacagc cagcgctcga tggttgccag atcgcggatg
      541 ccgccgccga gctgcaccgg aatggaaatg gctttcagga tttcctccac ggcccgggca
      601 ttgacgggct tgccttcgaa agcgccgttg aggtcgacga ggtgcagcca tgcgaagccc
      661 tggtcctcaa agctcttcgc ctgggcggcg ggattgtcat tgaaaatggt ggcctgctcc
      721 atgtcgccga gcttcaggcg cacgcattgg ccgtccttca gatcaatggc gggaaacagg
      781 atcatggctt ccaccgcagg aaattagcaa tcagcgccag gccgagaacc tggcttttct
      841 ccggatggaa ctgtgtgccg gcaacattgt cataacccac catggcggtc acgggcccgg
      901 cgtaatcggt ggtggccacc acatcttcag gaagggttgc ttcgagctgg taggagtgga
      961 cgaaataggc gtgaaggccg ttttctccca gcgctatgcc gtcgagcaag gcgtgggggt
     1021 ttttcacatc gagcgtgttc cagcccatgt gcggaatttt cagagtcctg tcggcgggcg
     1081 tgatcagttt cacgtcaccg gcgatccatc ccagccccgg cgttatgtca tgctcaaggc
     1141 cgcgcgtggc catgagctgc atgcccacac agatgccgag aaaaggtttg ccgcgggcga
     1201 tgacctgctc ctgcagcgtg tcgcgcatgc cgggcaccgc catcaatcct tcataacaat
     1261 ccttgaaggc gccgacaccg ggcaggacaa tgcgttcggc cttgcggacc acatccggat
     1321 cagcggtgac aatgatttgc ccgctggcgc ccgccgcgcg ctcgaaagcc ttcagggcgg
     1381 agtgcaggtt gccggaaccg taatcgataa tcgccacttt catggcgttc agcttctggc
     1441 gccgaagagg ccgagcacat cgtgggctgc cggcagtacc gtgggcgcgt gaagcgccgg
     1501 gacatcggca tcgccggcat aaaagcgcag ttcggcttct tccatgctgg cgccattgct
     1561 cagggcgatt tccgcatatt tgcgccgggt gagcgaccag cccagcaggt cattgccatg
     1621 gaggcccatg acaaggctga cggcaagcga cagcaaaaac tgcggcacgg tcggcaaggc
     1681 agaggcaatc agcatcaccg acagagccag cagggccacg atccacatgc ggttccacag
     1741 ggcccagacc caggtgaaga caaaggccct ccacgagaag ccgtcgggca ggaaaatcgc
     1801 gtcggcggct ccccggtcat cgggccttgc atatatcgaa tacgtcgtca tcagcctttg
     1861 agcgaaccct tggtggacgg aatgcgatcc ttctgccggg gatcaatctc gatggccccg
     1921 cgcagcacgc gggccagcgc cttgaaacag gattcggcga tgtggtggca attgtcgccg
     1981 tagagcgttt gcacatgcag cgtcacgccg gcattcatgg caaaggcctg gaaccattcg
     2041 cgcaccagtt cggtgtcgaa ctcgccgatc ttggggcgcg cgaattccac cttccagatg
     2101 aggaaggggc ggcccgagac atcaattgcc gcgcgagtga gcgcttcatc catgggcagg
     2161 aaacaatcgg catagcggcg gatgcccttg cggtcgccca gcgccttggc aaaggcctgg
     2221 cccagggcaa tgcctgtgtc ttccgtggtg tggtggaaat cgatgtgggt gtcgcccttg
     2281 gcgcggacct tgatgtcgat cagggaatgg cgcgccagca gctccagcat gtggtcgagg
     2341 aagcccacgc cggatttcac gtcataggcg ccggtgccat ccagattgat ggaaacggcg
     2401 atttgggttt ccgtggtttt gcggtctaaa ctggctttgc gcatgggggt ccactttcag
     2461 gctggcctct tagcagatgc agcaatatat gactatatcg acctgccgga atcgtt
//
LOCUS       DAORVN010000288         2512 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_40459_length_2512_cov_2.768010, whole genome shotgun sequence.
ACCESSION   DAORVN010000288 DAORVN010000000
VERSION     DAORVN010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2512
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40459_length_2512_cov_2.768010"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..535
                     /locus_tag="PKV23_06560"
     CDS             <1..535
                     /locus_tag="PKV23_06560"
                     /inference="COORDINATES: protein motif:HMM:NF016180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sarcosine oxidase subunit gamma family protein"
                     /protein_id="HQX85132.1"
                     /translation="SMPENFQRVSPLADIAIQGRFGADYGAPGVTLSVRHPALIATVI
                     ARRGKAKALGEALKNWRVQWAGAEQYFVLDRPFDEVRKKLDGLASCSDQSHGRVIIRI
                     EGPKVRQVLCKGTPVDLHERAFEIGNSALTQMAHVGVHLTRVERDAFELSVFRGFSES
                     FWEWLTEQAEEFGYQVI"
     gene            complement(537..1709)
                     /locus_tag="PKV23_06565"
     CDS             complement(537..1709)
                     /locus_tag="PKV23_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006468537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HQX85133.1"
                     /translation="MKLASITQRLSGLGSDKWAVHIEGKRRASAGEDLIFLSIGEPDA
                     PTPTSIMDVVNTQMRAGRYKYAASRGECGLLDGLSAQYSKSTGRPISTQQFIFLPGTQ
                     TALFVALQATVELGDEVLMADPYYATYEGVIAAAGGIAVPVAVDPDNGFHLQAADLER
                     AITPKTRALLLNTPSNPTGAVLTRETIAQIGDVCERHDLWIISDEVYATMTYGNTVFA
                     SPFDEKRLEKRTIVVSSLSKSHAMPGFRCGWLAASEDFIEAALPLTETMLFGSQAFLE
                     DAAAYAIANHFPEVDAMKQSYERRARALVAGLKGAKNISARMPEGGMFVMVDVRKTGL
                     SGEDFARRLLKEENVVTMPGESFGAGGSGHVRVALTVDEAQITEACKRIARLSAAL"
     gene            complement(1731..2141)
                     /locus_tag="PKV23_06570"
     CDS             complement(1731..2141)
                     /locus_tag="PKV23_06570"
                     /inference="COORDINATES: protein motif:HMM:NF014091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF126 domain-containing protein"
                     /protein_id="HQX85134.1"
                     /translation="MTVLHPGMAQGPVLLLDEPLSFWGAFDPRSGTIIDIHHPQRGQN
                     LNGKILMMLESRGSGSAPGGLAEAIRLGTAPAGIILVQPDINLAIGAAVAETLYGKAC
                     PVLAVTAAEFQELSKSAYLHISHDGRVGPSLDGA"
     gene            complement(2138..>2512)
                     /locus_tag="PKV23_06575"
     CDS             complement(2138..>2512)
                     /locus_tag="PKV23_06575"
                     /inference="COORDINATES: protein motif:HMM:NF016310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase X"
                     /protein_id="HQX85135.1"
                     /translation="DAVALGTPHFSLSEIAELVKLLDGRKVKAATYVSTSRGIREMLV
                     AKGWLKLLHDAGVIIPVDVCTYYSPKIQGLKGRVMTNAAKWAYYAPGMLGVDVCFGSL
                     KECVESAVRGEVWRDPALWAVP"
BASE COUNT          472 a          704 c          847 g          489 t
ORIGIN      
        1 ctccatgcct gagaattttc agagagtttc gccgcttgcg gacattgcca ttcaaggccg
       61 cttcggtgcc gattacggcg cgccgggtgt gaccctttcg gtgcggcacc ccgcgcttat
      121 cgccacggtg atcgcgcgca ggggcaaggc gaaggcgctt ggcgaggcat tgaaaaactg
      181 gcgcgtgcag tgggccgggg cggagcagta tttcgtgctg gaccggccct ttgacgaggt
      241 gcggaagaaa ctggatggcc tcgcctcctg ttccgaccaa agccatggcc gggtaattat
      301 caggatcgag gggccgaagg tgcgccaggt gctgtgcaag ggcacgccgg tcgacctgca
      361 tgagcgcgcg ttcgagatcg gcaatagcgc gctcacgcaa atggcccatg tgggcgtaca
      421 tcttacgcgc gtggagcggg acgctttcga gctctcagtg ttccgcggct tctccgaaag
      481 cttctgggaa tggctgacgg aacaggcgga agagtttggc tatcaggtga tttagttcag
      541 agcgccgccg acagcctggc aatcctcttg caagcctcgg tgatctgggc ttcatcgacg
      601 gtaagcgcca cacgcacgtg accggagccg ccggcgccga agctttcgcc gggcatggtg
      661 acgacgtttt cctccttgag aagccggcgg gcaaaatctt cgcctgaaag ccccgtcttg
      721 cgcacgtcga ccatgacgaa catgccgcct tcgggcatgc gggcggaaat attttttgcg
      781 cctttcaatc cggcaaccaa agcgcgggcg cggcgctcgt agctctgttt catggcatcc
      841 acttcgggga agtggttggc aatcgcataa gccgcggcat cctcaaggaa ggcctgggag
      901 ccgaagagca tggtttcggt gagcggcagt gcagcctcaa tgaaatcctc ggaggccgcc
      961 agccagccgc agcggaagcc gggcatggca tgggatttgg aaagggacga gacaacgatg
     1021 gtgcgctttt ccaggcgctt ctcatcaaag ggcgaggcaa acacggtgtt gccataggtc
     1081 atggtggcgt agacttcatc ggagatgatc cacaggtcat ggcgctcgca gacatcgccg
     1141 atctgggcaa tggtttcgcg tgtcagcacg gcaccggtgg ggttgctcgg tgtgttgagc
     1201 agcagggcgc gggttttcgg ggtgatggcg cgttcgagat cggcggcctg gagatggaag
     1261 ccattgtcgg gatcaacggc cacgggaacg gcgatgccgc ctgctgcggc gatgacgcct
     1321 tcataagtgg cgtagtaggg atcggccatc agcacttcgt cgccgagttc aacggttgcc
     1381 tggagggcca cgaacagggc ggtttgcgtg ccggggagga agatgaattg ctgggtgctg
     1441 atggggcggc cggtgctctt gctgtattgc gccgagaggc cgtcgaggag tccgcattcg
     1501 ccgcggctgg cggcatattt gtagcgcccg gcgcgcattt gggtgttgac cacatccatg
     1561 atggatgtgg gggtgggcgc atcgggctcg ccgatcgaaa ggaatatcag gtcctcgccg
     1621 gcgctggcgc ggcgcttgcc ttcgatgtgc acggcccatt tatcggagcc gaggccgctg
     1681 aggcgctggg tgatggatgc aagtttcatg ggggccttct agccgagcct tcaggcaccg
     1741 tcgagggatg gcccgaccct gccgtcatgg gagatatgca aataggctga cttcgaaagc
     1801 tcctgaaatt ccgcggccgt gacggccaga accgggcagg ccttgccata aagcgtttcg
     1861 gccacggcag cgccgatggc gaggttgatg tcgggttgca ccaggatgat cccagcaggg
     1921 gccgtgccaa gcctgatggc ttcggccaag cctccggggg cgctgccgga accccggctt
     1981 tccagcatca tgaggatttt tccattaaga ttctgtcccc gttgcggatg gtgaatgtcg
     2041 ataatggttc cgctgcgcgg atcgaaggcg ccccagaagc tcaggggttc atcaaggagg
     2101 agaaccgggc cctgcgccat gcctggatga agcacggtca tgggactgcc caaagcgctg
     2161 gatcgcgcca gacctcgccc cgcacggcgc tttccacgca ttccttcaag ctgccgaagc
     2221 agacgtcaac gcccagcatt ccgggtgcgt aataggccca tttcgccgca ttggtcatga
     2281 cgcgaccctt gagcccctga atcttgggcg aatagtaggt gcagacatcg acggggatga
     2341 tgacgccggc gtcgtgcaga agcttgagcc agccttttgc aaccagcatt tcgcggatgc
     2401 cgcggctggt ggaaacatag gtggcagctt tcaccttgcg cccgtccagc aatttcacca
     2461 gttcggcaat ctcggaaagc gaaaaatgcg gcgtaccaag ggcaaccgca tc
//
LOCUS       DAORVN010000289         2510 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_40507_length_2510_cov_3.086762, whole genome shotgun sequence.
ACCESSION   DAORVN010000289 DAORVN010000000
VERSION     DAORVN010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2510
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40507_length_2510_cov_3.086762"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1640)
                     /gene="leuA"
                     /locus_tag="PKV23_06580"
     CDS             complement(<1..1640)
                     /gene="leuA"
                     /locus_tag="PKV23_06580"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018857590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HQX85136.1"
                     /translation="MMISPKEKYRAYPPVSLPDRTWPEKAITKPPIWCSVDLRDGNQS
                     LIEPMGHERKLRMWNALVKMGFKEIEIGFPSASQTDFDFCRFLIDNKKIPDDVTVQVL
                     VQCREDLITRTFEGIRGAKSVIVHFYNSTSELQRRVVFNKDKAGITEIAANAARLIKK
                     LTQASGMEEAIRFQYSPESFTGTELDYAVEICEAVMDEIRPTPQKPLILNLPATVEMS
                     TPNIHADQIEWFCRQIKNRSSVIISLHPHNDRGSAVAATELALMAGADRVEGTLFGNG
                     ERTGNVCLVTLAMNLYTQGVDPGLDISNINDLIRMSEYCTQLPVHPRHPYAGELVFTA
                     FSGSHQDAINKGFKAMKASNKPLWEVPYLPIDPMDLGRSYEAVVRINSQSGKGGIAYI
                     LETEHGIELPRRLQIEFSKVVQAIADGEGVELSAERLWRSFEGEYLEGNGRFGFIEHN
                     AKSDHGDQDLTARISDDGKHKTIHGHGNGPVDGFVDAMRKESGLAFDVADYREHAMGH
                     GANAAAIAYVELRLADGSTLFGVGIDKNIVVASLKAVVS"
     gene            1918..2160
                     /locus_tag="PKV23_06585"
     CDS             1918..2160
                     /locus_tag="PKV23_06585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85137.1"
                     /translation="MKSLILAAALTLAVSGAALAGQCQDDIEKIDAALAGGDVSPDQR
                     AQLEDMRNQAVQLCGAGNEAEGLDVTAEAKAMLNIQ"
     gene            complement(2163..>2510)
                     /locus_tag="PKV23_06590"
     CDS             complement(2163..>2510)
                     /locus_tag="PKV23_06590"
                     /inference="COORDINATES: protein motif:HMM:NF014609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HQX85138.1"
                     /translation="PHWSALIRPMGGVDAAYVYTRGGDNPKTDFRARMFAPTGGIPED
                     PATGSATALLAAQLLKAEALKDGTHKWQLEQGYEMGRPSDLFLEADVAGGALKAVRVA
                     GQAVKVMQGTLEI"
BASE COUNT          470 a          747 c          778 g          515 t
ORIGIN      
        1 gatacaaccg ccttcagcga ggcaacgaca atgttcttgt cgatgccgac gccgaacagc
       61 gtcgagccat ccgccagccg cagttccaca taggcgatgg ccgccgcatt ggcgccgtgg
      121 cccatggcgt gttcgcggta gtccgccacg tcgaaggcaa gccccgactc tttccgcatg
      181 gcgtcgacaa agccgtcgac cgggccgttg ccatggccgt gaatggtctt gtgcttgccg
      241 tcatcgctga tccgggcggt gagatcctgg tcgccatgat ccgacttggc gttgtgttcg
      301 atgaagccga agcggccgtt gccttcaaga tattcgccct cgaagctgcg ccacaagcgc
      361 tcagccgaaa gctccacgcc ctcgccatcg gcaatggcct gcaccacctt ggagaattca
      421 atctgcagcc gccgcggcaa ttcaatgccg tgttcggttt ccaggatata ggcgataccg
      481 cccttgccgc tttgcgaatt gatccgcacc acggcctcgt aggagcggcc caggtccatg
      541 gggtcgatcg gcaggtaagg cacttcccac aacggcttgt tggaggcctt catggccttg
      601 aagcccttgt ttatggcgtc ctggtgcgag ccggaaaacg ccgtgaacac cagttcgccc
      661 gcataggggt ggcgcgggtg caccggcaac tgcgtgcagt attccgacat gcggatgaga
      721 tcattgatgt tggagatgtc gaggccggga tcaacgccct gcgtgtaaag attcatggcc
      781 agcgtgacga ggcatacatt gccggtgcgc tcgccattgc cgaagagcgt gccctccaca
      841 cggtcggccc cggccatgag ggcgagctcc gtggcggcga cggccgagcc ccggtcattg
      901 tgcggatgaa gcgaaatgat gacggagctg cggttcttga tctggcggca gaaccactcg
      961 atctggtcgg cgtggatgtt gggagtcgac atctcgacgg tggcggggag attgaggatc
     1021 agtggctttt gcggcgtggg acggatttca tccatcaccg cctcgcagat ttccaccgca
     1081 tagtcgagct cggtgccggt gaagctttcc ggcgaatact ggaaacggat ggcctcttcc
     1141 attccgcttg cctgcgtgag cttcttgatg aggcgcgcgg cattggcggc gatttcggta
     1201 atgccggcct tgtccttgtt gaagacgaca cggcgctgca gttcgctggt ggagttgtag
     1261 aaatggacga tcaccgactt ggcgccgcgg atgccttcga acgtgcgggt aatcaggtcc
     1321 tcgcggcatt gcaccagcac ctgcacggtg acgtcatcgg ggatcttctt gttgtcgatg
     1381 agaaagcggc agaaatcgaa atccgtctgc gaagcggagg gaaaaccgat ctcgatttcc
     1441 ttgaagccca ttttgaccag cgcgttccac atccggagct tgcgctcatg gcccatgggc
     1501 tcgatcagcg actggttgcc gtcacggagg tccacgctgc accagatcgg cggcttggta
     1561 atggcttttt ccggccaggt gcggtccggc aaggatacgg gcggataggc ccggtatttt
     1621 tctttggggg aaatcatcat ggtctgagtc tcttaaatct tggctgttgg gtttgtctgc
     1681 tgattctttt ccccaaagag ccaaggcaaa gcccagccgg ccctcagggg gagctaagaa
     1741 ggaggagctg gttcagcttg cgagacatgg ggtttccata gacgatgaat tgccaaagcg
     1801 caacacctgt ggcttttacg ccgcattaat gatgtttttc cggccacacg gaatcaggct
     1861 aagttcggtc attattggct gcgagaagcg ccacatgcat ttggggacct gcacatcatg
     1921 aagagcctga ttctagccgc cgcgcttaca cttgccgttt cgggcgccgc cctggccggg
     1981 caatgccagg acgatatcga aaaaatcgat gcggcccttg ccggcggcga tgtgagcccc
     2041 gaccagcgcg cccagcttga agacatgcgc aaccaggcgg tccagctttg cggtgcgggc
     2101 aatgaggccg aaggccttga cgtcaccgcc gaagccaagg ccatgctcaa tatccagtag
     2161 gcctaaatct ccagcgtacc ctgcatgacc ttcaccgcct gcccggccac gcgcaccgct
     2221 ttcagcgcgc cgcccgccac atccgcctcg aggaacaggt cggacggacg gcccatctca
     2281 tagccctgct ccagctgcca cttgtgggtg ccgtccttga gagcttcggc tttcaacagc
     2341 tgggccgcca acagcgccgt ggcagacccg gtcgccgggt cctccggaat gccgccggtg
     2401 ggggcaaaca tccgggcgcg gaaatccgtc ttgggattgt cgccgccgcg tgtatagaca
     2461 taggctgcat ccaccccgcc catgggcctt atgagggccg accaatgggg
//
LOCUS       DAORVN010000290         2502 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_40763_length_2502_cov_3.080098, whole genome shotgun sequence.
ACCESSION   DAORVN010000290 DAORVN010000000
VERSION     DAORVN010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2502)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2502)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2502
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40763_length_2502_cov_3.080098"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..663
                     /locus_tag="PKV23_06595"
     CDS             <1..663
                     /locus_tag="PKV23_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011425391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HQX85139.1"
                     /translation="RAWPSFFTTLWLAAAAAPLTAAIGLLTAYLLNRQKFIGQSTFEF
                     ITMLSFAIPGTVIGVSYILVFNVPPIEITGTGLILIICFVFRNMPVSIRAGIATMSQI
                     DRSLDECSLMLRHGSFATIRRVILPLLRPAIIASLVYAFVRAITSVSAVIFLVSAQYN
                     LATAYIVGRVEVSDFGVAIAYSSVLIIFMLLAIGAIQLGVGEQRRSSRGVQPMAVHGG
                     VA"
     gene            660..1736
                     /locus_tag="PKV23_06600"
     CDS             660..1736
                     /locus_tag="PKV23_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007879519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQX85140.1"
                     /translation="MKIVSRSAAVRLDGVTKAFGKTIAVDAVTLDIEAGTLVTLLGPS
                     GCGKTTTLRMIAGLETQTSGHVLIGGKDVTLLPPNERDVSMVFQSYALFPHMSVIENV
                     SYGLRQSQLSRASQRDKAREGLKLVGLEGYDDRLPSELSGGQQQRVAVARALVLEPQV
                     LLFDEPLSNLDAKLRRRMREEIRDLQQTLGLTSVYVTHDQEEALAVSDKIVVMNMGRI
                     AQVGTPHDLYERPADAFVADFIGSANLIPGSIRKRNGKEKTITIDLGNLSVEVPDRGY
                     EGNEIYLVVRPSGVSLDAKGSKAASLPAKVEKATYLGSHWEYTITTANSSMFVIQPTH
                     KIFKAGARVFATLDPDHITVVRRG"
     gene            1851..>2502
                     /locus_tag="PKV23_06605"
     CDS             1851..>2502
                     /locus_tag="PKV23_06605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85141.1"
                     /translation="MQNPSYSYMLPQPAGLPVLTSTVNPAVRAPVEVLTQFGKIELLW
                     REMVQHGALCSRYQHHSWMAGWWDHIGSMSQDSPHVTVLRDDMGRPVLLLPLVRKKVG
                     PLQAGFFMGGKHTNFNLPVWRPGHLKQPTAALNALVEGLRHNGPRLDLLVLLNQPAAW
                     QGVANPMLQAPHREAASRAYTGELQSDFEALLKERMGANSRKKLRQKERQLAALGQV"
BASE COUNT          457 a          768 c          779 g          498 t
ORIGIN      
        1 cgggcgtggc cttccttctt cacgacgctc tggctcgccg cggcggctgc gcctttgacc
       61 gcagcaatcg gtttgctgac cgcctatctt ctcaaccggc agaaattcat cggccaatcc
      121 actttcgagt tcatcaccat gctgagtttc gccattcccg gcacggtgat cggggtgagc
      181 tacatcctcg tcttcaacgt gccgcccata gaaatcacgg gcaccggcct catcctcatc
      241 atctgctttg ttttccgcaa catgccggtt tcaatccggg cgggcatagc gacgatgagc
      301 cagatcgacc ggagccttga cgaatgctcg ctgatgctgc gccacggaag ctttgccacg
      361 atccgccggg tgatcctgcc gctgctcagg ccggcgatta tcgcatcgct ggtctatgcc
      421 ttcgtgcggg ccatcacctc ggtgagcgcc gtgatcttcc tggtgagcgc gcaatacaat
      481 ctcgcaacgg cctatatcgt cgggcgggtg gaagtttcgg actttggcgt cgccattgcc
      541 tacagctcgg tacttattat cttcatgctg ctggcgatcg gggccattca gcttggggtg
      601 ggcgagcagc ggcgttccag ccgcggggtt cagcccatgg cggttcatgg gggtgttgca
      661 tgaaaattgt cagccggtcg gcggcggtca ggcttgatgg cgtcaccaag gcatttggaa
      721 agacgatcgc cgtcgatgcg gtcacgctcg acattgaagc gggcacgctg gtgacactgc
      781 tcgggccctc gggctgcggc aaaaccacga cgctgcggat gattgccggc cttgaaacgc
      841 aaacctcggg ccatgtgctg atcggcggga aggacgtcac ccttctgccg ccgaacgagc
      901 gcgacgtcag catggtgttc cagtcctatg ccctgttccc gcacatgagc gtcatcgaaa
      961 atgtgtccta cggactgcgg cagtcgcagc tctcgcgggc ctcccagcgc gacaaggcgc
     1021 gcgaaggcct gaagctggtt gggctcgagg gctacgatga ccgcctgccc tcggaattgt
     1081 ccggcgggca gcagcagcgc gtggcggtgg cccgggcgct ggtgctggag ccgcaggtgc
     1141 tgctgtttga cgagccgctt tccaatcttg atgcgaaact tcgccgccgg atgcgggaag
     1201 agatcaggga cttgcagcag accctggggc tcaccagtgt ctatgtgacc cacgaccagg
     1261 aagaggcgct ggcggtttcg gacaaaattg tcgtcatgaa catgggccgc atcgcccagg
     1321 tgggaacgcc gcatgatctg tatgagcggc cggcggatgc ctttgtcgcg gacttcatcg
     1381 gcagcgccaa tctcattccg ggcagcatca ggaaacggaa cgggaaggaa aagaccatca
     1441 cgatcgacct tggcaacctg agcgtcgagg tgccggaccg gggctatgag ggcaacgaga
     1501 tttaccttgt ggtgcgcccc agcggcgtca gcctggacgc caagggctca aaggccgcat
     1561 cgcttccggc aaaagtcgag aaggccacct atctcggatc gcactgggaa tacacgatca
     1621 ccacggccaa cagttcgatg ttcgtgatcc agccaaccca caagatattc aaggcgggtg
     1681 cccgggtttt tgccaccctt gacccggatc acatcacggt tgtgcggcgc ggctaattgc
     1741 gttgcccccg tgtgaagatt caaggttaac tggcacaggg cttgccttgt taagggcaag
     1801 cggccgccgt tggggggccg cgccgtttca cccgttcgag gcccggaact gtgcaaaatc
     1861 cttcctactc ctatatgctg ccgcaacctg ccggccttcc ggtgctcacc tcaacggtta
     1921 accctgcggt gcgggcaccg gtcgaagtcc tgacccaatt cgggaagatc gagctcctgt
     1981 ggcgcgaaat ggtacagcac ggagccctct gcagccgcta ccagcatcat tcctggatgg
     2041 cgggctggtg ggatcacatt ggaagcatgt cccaggattc gccccatgtc acggtgctgc
     2101 gcgacgatat gggcaggccc gttcttctgc tgccgctggt ccgcaaaaaa gttggccccc
     2161 ttcaggccgg atttttcatg ggcggcaagc acacgaattt caatctgccg gtctggcgtc
     2221 cgggacatct gaagcagccc accgccgcac tgaatgcgct tgtcgagggc ttgcgccaca
     2281 acgggccgcg cctcgatctt ctcgtgcttc tgaaccagcc cgcggcctgg cagggcgtgg
     2341 ccaatcccat gctccaggcg ccgcaccggg aagctgccag ccgggcctat acgggcgagc
     2401 tgcaaagtga tttcgaagcc ttgctgaagg agcgcatggg tgccaattcg cgcaagaagc
     2461 tccggcagaa ggagcgccag cttgcggccc ttgggcaggt tc
//
LOCUS       DAORVN010000291         2494 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_41010_length_2494_cov_3.115621, whole genome shotgun sequence.
ACCESSION   DAORVN010000291 DAORVN010000000
VERSION     DAORVN010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2494)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2494)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2494
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41010_length_2494_cov_3.115621"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..566
                     /locus_tag="PKV23_06610"
     CDS             <1..566
                     /locus_tag="PKV23_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="signaling recognition particle receptor family
                     protein"
                     /protein_id="HQX85142.1"
                     /translation="KLGSIATGEGFKVMFAACDTFRAAAIEQLTIWGERIGARTLSRP
                     TGSDSSGLAYDAMQTAKADGTDILFIDTAGRLQNKAYLMDELDKIVRVVKKQDATAPH
                     AVLLILDATTGQNAVSQAEIFTKVAGVTGLIMTKLDGTARGGILVAIAEKFALPIHAI
                     GVGESIDDLQPFDADAFARAISGLAKT"
     gene            563..1132
                     /locus_tag="PKV23_06615"
     CDS             563..1132
                     /locus_tag="PKV23_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014330142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HQX85143.1"
                     /translation="MKPLPKFLIEMGPLLAFFVGNWKGGVFWGTGIFMVATAVALAAS
                     WILTRKIAKVPLFSAIFVGIFGGLTIWMHDDTFIKVKVTLINVFFGALLLGGLYFGKL
                     FIRYVVGEAVNLPTAAWRVLTLRWGIFFLVVAMLNELIWRNVSTDTWVNFKVFGLLGL
                     TLVFALANAPYMAKHMIEDETPKPDAPSA"
     gene            1386..1886
                     /locus_tag="PKV23_06620"
     CDS             1386..1886
                     /locus_tag="PKV23_06620"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HQX85144.1"
                     /translation="MSGNRDVKQLSRSLSHLLKRAVQYSIHLYMSETGKTGLTHRQFT
                     VLLAADAHEGRSQTELVKMTGIDRSTLADLVARLMAQGYVQRRRTKDDARTNAIRLTP
                     IGKKMLKQAQSGAEEVDRQLLAILPSSDRKTLLECLTGLAAEMDKVEDKEPEKPVRKV
                     RPRKGR"
     gene            complement(1887..2453)
                     /locus_tag="PKV23_06625"
     CDS             complement(1887..2453)
                     /locus_tag="PKV23_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013167573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbE family thiol:disulfide interchange protein"
                     /protein_id="HQX85145.1"
                     /translation="MTSGSPRRRLGLAFLPILIFAGLALVFWKGLSGDPSNLPSALIG
                     KPAPDFSLSALEGSNIPGLTSVDLKTGGVSVVNIWASWCGPCRLEHPILMELSRRTDV
                     RLYGINNKDKPENARRFLGTLGQPFAAIGADSSGRTTIDWGSYGVPETFIVDGKGVIR
                     FKWIGPLSAEIMAGTFAGEIEKAKQPLP"
BASE COUNT          506 a          716 c          792 g          480 t
ORIGIN      
        1 gcaagctggg ctccattgcc acgggcgaag gcttcaaggt gatgtttgcc gcctgcgaca
       61 cgttccgcgc cgccgccatc gagcagctca ccatctgggg cgaacgcatc ggcgccagga
      121 ccctgtcgcg gcccacgggc tccgattcgt cggggctggc ctatgatgcg atgcaaaccg
      181 cgaaggcgga cggcaccgac atcctgttca tcgacacggc ggggcgcctg cagaacaagg
      241 cctatctcat ggacgagctc gacaagatcg tgcgggtggt caagaagcag gatgcaacgg
      301 cgccgcatgc cgtattgctg atcctggatg ccacgacggg gcagaacgcc gtttcgcagg
      361 cggaaatctt caccaaggta gcgggcgtca ccgggctgat catgaccaag cttgatggca
      421 cggcgcgcgg tggtatcctt gtggcgatcg ccgaaaaatt tgcgctcccc atccacgcca
      481 tcggcgttgg cgagagcatc gacgaccttc aacccttcga cgcggacgct tttgcccgcg
      541 ccatttcagg actggcaaag acatgaaacc acttcccaaa ttcctcattg aaatgggccc
      601 gctgcttgcc ttcttcgtgg gcaactggaa gggcggggtg ttctggggca cgggcatttt
      661 catggtggcg accgccgtgg cccttgccgc ctcatggatc ctgacacgaa agatcgccaa
      721 ggtgccgctg ttcagcgcca tcttcgtcgg catcttcggc gggcttacaa tctggatgca
      781 cgacgacacc ttcatcaaag tgaaggtgac gctgatcaac gtgttcttcg gcgcgctgct
      841 tctcggcggg ctctatttcg gcaagctgtt catcagatac gtggtaggcg aagcggtcaa
      901 cctgcccacc gcggcatggc gcgtgctcac cctgcgctgg ggcatctttt tcctggtggt
      961 ggccatgctg aacgaactga tctggcgcaa tgtcagcacc gacacgtggg tcaatttcaa
     1021 ggtgttcggt ttgctggggc tgaccctggt attcgcactt gcgaacgcgc cctatatggc
     1081 caagcacatg atcgaagacg agaccccgaa gccggatgcg ccgtccgctt gagcgggcaa
     1141 ttttcagaag tcaagagttg taatccgtgt ccggatgatt ttaggtattt tgccatgggg
     1201 caagcgtacg ctttttcgct tgccgcttcg gaattgaaac gctaggactc accgtctttc
     1261 tgaaagcagg gcgcagccat catcgcaggg gcgggaagat ggcgcgacgg gcggaaggca
     1321 atggggcaag cagcacagca ggggatcaaa atccgtttga ttcaccggcg caggagtagg
     1381 ggccaatgag cggcaacagg gacgtcaagc aactgtcgcg atcactctcg catttgttga
     1441 agcgggcagt gcaatactcc attcatcttt acatgagcga gaccggcaag accggcctga
     1501 cacatcgtca gttcacggtg ttgttggcgg ctgacgccca tgaggggcgc agccagacgg
     1561 agctggtgaa gatgaccggc atcgaccgtt cgaccctggc cgatctcgtg gcccgcctca
     1621 tggcgcaagg ctatgtgcaa cggcggcgca ccaaggacga tgcgcgcacc aatgccatca
     1681 ggctcacccc catcggcaag aaaatgctga agcaggccca gtcgggggcg gaggaagttg
     1741 accgccagct tctggccatt ttgccgagtt cggaccgcaa gacgctgctg gaatgcctca
     1801 caggccttgc cgcggaaatg gacaaggtgg aagacaagga accggaaaag ccggtgcgga
     1861 aggtccggcc gcgcaagggc cgctgatcaa ggaagaggct gtttggcctt ttcgatttcg
     1921 ccggcaaaag tgcctgccat gatttccgcc gagagcgggc cgatccattt gaaacggatg
     1981 acccccttgc catcaacgat gaaggtttcg ggcactccgt agcttcccca gtcaattgtg
     2041 gtgcgcccgc tgctgtcagc gccgatggcg gcgaagggct ggcccaaggt gccgaggaag
     2101 cgcctggcgt tttcgggctt gtccttgttg ttgatgccgt agaggcggac gtcggttctc
     2161 cgggaaagct ccatgaggat gggatgttca agtcggcagg ggccgcacca cgaggcccag
     2221 atgttcacca cggagacgcc gccggttttg agatcaactg aggtgaggcc ggggatgttt
     2281 gaaccttcga gtgccgagag ggaaaagtcc ggcgcgggct tgccgatcag ggctgagggc
     2341 aggttcgagg gatcgccgga cagtcccttc cagaaaacca aggcgaggcc cgcaaaaatc
     2401 aggatgggca gaaacgccag gcccagccgc cgccgtggag atccggacgt cactttttta
     2461 gtttctcaaa ttgcgccagc cgtttcctca cccg
//
LOCUS       DAORVN010000292         2492 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_41081_length_2492_cov_2.365613, whole genome shotgun sequence.
ACCESSION   DAORVN010000292 DAORVN010000000
VERSION     DAORVN010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2492
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41081_length_2492_cov_2.365613"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(53..205)
                     /locus_tag="PKV23_06630"
     CDS             complement(53..205)
                     /locus_tag="PKV23_06630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85146.1"
                     /translation="MMRKLLTAVLFVLLSLSVAPAQSNVPASTSQDEVLMIQKKKKLA
                     TLKAAE"
     gene            342..1550
                     /locus_tag="PKV23_06635"
     CDS             342..1550
                     /locus_tag="PKV23_06635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85147.1"
                     /translation="MMNSPRAALFLLLAATALTVPARAQDVPPVVTAIFKSWETQLKA
                     TPAYDKIETDSSGNVTISNVAATVSAQDPGTTMKLSIGSIALENVATEAEGLIAVGAA
                     TFSDTKLEFTGPQNKSFVIDIPKASAEDWYVAVLGANPTPLQVFRGSMGIAKKMTSGE
                     IKITAEGQTFTAKGIESAWSGDPVTGAGKTTMSFNDLVIPESAIALLDPAGQLKALGY
                     SGLTLNAGGEGEFTVTGESFGMSGTINIASKDMAGLTFSYGASDIPIAVMTEMQAAQK
                     SGRPPDFNMLMPQLMNVSLSGFTVRFEDASITKKVLPIIAQMQGMDQAAMVANAGAMM
                     QLSLMQLRNQAFTDQVVAAVNSFLKDPKSFTVSFKPAAPVKVEQVMALDPANPGAAVD
                     VLGVAVTAND"
     gene            complement(1669..>2492)
                     /locus_tag="PKV23_06640"
     CDS             complement(1669..>2492)
                     /locus_tag="PKV23_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018698151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HQX85148.1"
                     /translation="DCPIPAAEAGSRALCDRMNSWAQQQGQPGLGYMIIKDGQGTGPI
                     AKNLGPERSEAIRKQIHLADDYCGGAAEGDAIFFVAGRPEKFYKFAGEARTRIIEETG
                     LAEKNCFKFCWIVDFPMYEWNEDDKKVDFSHNPFSMPQGGLAALLEKDPLEILAYQYD
                     IVCNGVELSSGAIRNHRPDVMKKAFEIAGYPEEVLLEKFGGMYRAFQYGAPPHGGIAP
                     GVDRIVMLLCNEPNIREVVLFPMNQQAEDLLMGAPSEATPKQLRELHIRLNLPEA"
BASE COUNT          527 a          743 c          715 g          507 t
ORIGIN      
        1 aaatagccgg catgcaacca ggccagggcg gtccgcgggc cgccttcttt ttttactcgg
       61 ccgccttcag ggtcgccagt ttcttcttct tttgaatcat aagcacttca tcctggctgg
      121 tactggccgg gacattggac tgggccgggg cgaccgacag gctcagcaaa acaaataaca
      181 ccgccgtaag tagtttgcgc atcatggttt ttccctaaga agaagagcgc gaaaagtagc
      241 catttaaggg tgattcagca agtgggttga gttcacctgc ggttcactta tgctcgggca
      301 tagagcaatc ccgttgacaa cctcctaaga aggcaaaccc catgatgaat tccccccgcg
      361 ccgccctatt ccttctgctt gccgccacgg ccctgacggt tccggcccgg gcgcaggatg
      421 tgccgccggt cgtcacagcc attttcaaga gctgggaaac ccagctcaag gccacgcccg
      481 cctatgacaa gattgaaacc gacagttccg gcaacgtgac catcagcaat gtggccgcca
      541 cggtctcggc ccaggatccc ggcacgacga tgaagctgag catcggctcc attgccctgg
      601 agaatgtcgc cacggaagcg gaggggctca tcgcggtggg ggcggcaacc ttctctgaca
      661 caaagctgga attcaccggg cctcaaaaca agagctttgt cattgatatc cccaaagcct
      721 cggcggaaga ctggtatgtc gcggtcctcg gcgccaaccc cacgcctctg caggtcttcc
      781 gcggctccat gggcatcgcc aagaaaatga cctcgggcga aatcaagatc acggcggagg
      841 gccagacctt cacggcgaag ggaattgaat cggcgtggag cggcgatccg gtcaccggtg
      901 ccggcaagac caccatgagc ttcaatgacc tggtcatccc ggaatccgct atcgcattgc
      961 tggatccggc gggccagctg aaagccttgg gctacagcgg cctcaccttg aatgccggcg
     1021 gggaaggcga attcacggtc accggcgaga gcttcggcat gtcaggcacg atcaacatcg
     1081 cgagcaagga catggccggc cttaccttct cctacggagc gagtgacatt ccaatcgcgg
     1141 tgatgaccga aatgcaggcg gcgcagaaat cgggacggcc accggacttc aacatgctca
     1201 tgccacagct gatgaatgtg tcgctgagcg gcttcacggt tcgtttcgag gatgcctcga
     1261 tcaccaagaa agtgctgccg atcatcgccc agatgcaggg catggatcag gcggccatgg
     1321 ttgccaatgc cggggccatg atgcagctca gcctcatgca actgaggaac caggccttta
     1381 ccgaccaggt ggtggcggcg gtcaactcct tcctgaagga ccccaagtca tttaccgtgt
     1441 ccttcaagcc cgcggcgccg gtgaaggtcg agcaggtcat ggcgcttgat ccggccaatc
     1501 cgggcgccgc agtagatgtg ctcggggttg ccgtcacggc gaacgactga gtaatacctg
     1561 aacacaaaac actcatcatg cccgcgcttg acgcgggcat cttctttttt ggaggaccgc
     1621 agaagatggc cggatcgagt ccgaccatga tgagaaaccc tgggagcgct aggcttccgg
     1681 caaattcagc ctgatgtgga gttcacgcaa ttgcttgggc gttgcttcgg acggcgcgcc
     1741 catcagcagg tcttcggcct gctggttcat ggggaagagc acgacctcgc ggatgttggg
     1801 ctcgttacag agcagcatga caatgcggtc cacgcccggc gcaatgccgc cgtggggcgg
     1861 cgcgccatac tggaaagccc ggtacatgcc gccgaatttt tccagcagaa cttcctcggg
     1921 gtaaccggca atctcgaagg ccttcttcat cacgtcgggc cgatggttgc ggatggcgcc
     1981 ggatgagagc tctaccccgt tgcagacgat gtcatattga tacgccagaa tctccagtgg
     2041 atctttttcc agcagagccg cgaggccgcc ctgcggcatg gagaaggggt tgtgcgagaa
     2101 gtccaccttc ttgtcgtcct cgttccattc atacatgggg aagtcgacga tccagcagaa
     2161 cttgaagcag ttcttttctg cgagacccgt ctcttcgatg attctagttc ttgcttctcc
     2221 agcgaatttg tagaatttct ccggcctgcc ggctacaaag aaaattgcat caccttctgc
     2281 cgcaccacca cagtagtcat cggctaagtg aatctgtttg cgaatcgcct ccgagcgttc
     2341 tggacctaag ttcttggcaa tcggaccagt tccctgcccg tccttgatta tcatataccc
     2401 aagaccaggc tgcccctgct gctgagccca ggaattcatc cggtcgcata atgcccgtga
     2461 tccagcctcc gcagcgggga tggggcagtc ct
//
LOCUS       DAORVN010000293         2491 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_41096_length_2491_cov_3.854269, whole genome shotgun sequence.
ACCESSION   DAORVN010000293 DAORVN010000000
VERSION     DAORVN010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2491)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2491
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41096_length_2491_cov_3.854269"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            48..302
                     /locus_tag="PKV23_06645"
     CDS             48..302
                     /locus_tag="PKV23_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011581590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HQX85149.1"
                     /translation="MITAPQLRAARALLGIDQQKMAELAGVSLPTIQRMEASPDNIRS
                     VVSSLMKVLEALDKAGLELIGDGATSSGGGRGVRFKESSK"
     gene            299..2335
                     /gene="hyfB"
                     /locus_tag="PKV23_06650"
     CDS             299..2335
                     /gene="hyfB"
                     /locus_tag="PKV23_06650"
                     /inference="COORDINATES: protein motif:HMM:NF005086.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HQX85150.1"
                     /translation="MTGVDYSTAPQLAILLSGIVLYAAAAVFAAMFHRHKSALAAVYP
                     ICIIAAAVSAGASLAALLADTHSGFRLPLGLPIIGLHVKLDALSAFFSLIVNLGVLTT
                     AIYGMGLDRDRDLSGRVEPFFPAYALAMNLVLIAGDAFGFLFSWELMSLTSWVLVVSH
                     HRDAGNRHAGHVYLVMAAIGTASLLFAFGGLAGASGNYAFDAIRAVPPGPLVSGLVLA
                     GAIFGAGSKAGIAPLHAWLPLAHPAAPSHVSALMSGVMTKVAIYGLIRIVFDLLGTPV
                     WWWALPFLLLGAATAVLGLLYAVLDHDLKRVLAYSTVENIGIIFVGLGLALAFKASGL
                     TAAAAVAMAATLLHCLNHSWFKSLLFLGAGAVLHATGRRDFDGLGGLIHRMPVTAAFW
                     LVGAMSISALPPFNGFVSEWLLFQAVLAGPQFPEPILRFMSPAVGAMLALAAALAAAC
                     FVRAFGIVYLGRPRSNEAAGAHETARPQQVAMALLAGLCLLGGLFGGVMVSAIQPLLQ
                     TLAGAPLPAVGTGPTPLSLVAFDAARSIYDAPTIAIFLLISGTLTVMLVHRISSRRTR
                     LGPAWDCGFPDPSPLTQYSASSFSQPLRRVYGSTVFGASETVDMPQPGDNRAARFTVR
                     LHDYAWELLYAAPARAVLKMSERLNALQFLTIRTYLMLMFSALIVLLLIAAVWF"
BASE COUNT          382 a          777 c          723 g          609 t
ORIGIN      
        1 atcgatgatg cataatacgt ctatagttga gatgtgttaa ctccaccatg attactgcac
       61 cccaattgcg cgccgcccgt gccttacttg gcatcgatca gcaaaagatg gctgaattgg
      121 ccggcgtatc cttgcctaca atccagcgca tggaagcaag ccccgacaat atccggagtg
      181 tggtttcgag cttaatgaaa gtccttgagg cgctcgacaa ggcgggcctt gaattaatcg
      241 gtgatggtgc cacgagcagc ggcggcggcc gaggcgtccg gttcaaagag tcctccaaat
      301 gaccggcgtg gattattcca ctgctccaca gcttgcgatt ctgctgtcag ggatcgtgct
      361 ctatgcggcg gcggccgttt ttgccgccat gttccatcgc cataagtcgg cgctagctgc
      421 agtttaccct atctgcataa ttgcggcagc ggtgagtgcg ggggctagcc tcgcggcact
      481 gcttgctgac acacattcag gattcagact gccgcttggc ctgccaatca tcgggctgca
      541 cgtcaagctt gatgctctgt cggccttttt cagcctcatt gtgaatctgg gcgtgctgac
      601 gaccgcgatt tacggcatgg gacttgaccg cgaccgggac ctttcggggc gggttgaacc
      661 gttcttcccg gcatacgcct tggccatgaa cctcgtcctc atcgccggag atgcctttgg
      721 cttcctgttt tcctgggaat tgatgtcgct cacttcatgg gttctcgttg tctcgcacca
      781 ccgggacgcg ggcaaccgcc atgcggggca tgtctatctg gtgatggcgg caattggcac
      841 ggcttcgctg ctgtttgcct tcggtgggct tgccggtgct tccggaaatt acgccttcga
      901 cgcgatccgt gccgttccac ccgggcccct ggtttccggg ctcgtgctcg ccggtgccat
      961 tttcggggcc ggctcgaagg ccggcatcgc acctctccac gcctggctgc cgctggcaca
     1021 tccggctgcc cccagccatg tctctgccct gatgagcggg gtcatgacca aagttgcaat
     1081 ctacggcctg atccgcatcg tcttcgacct gctgggaaca cccgtctggt ggtgggccct
     1141 gcccttcctg cttctcggtg ccgcgacggc ggtacttggt cttctttacg ccgtgcttga
     1201 tcatgattta aagcgcgtcc ttgcctattc gacagtggag aatatcggta tcatcttcgt
     1261 gggccttggc ctggcgctgg ctttcaaggc ctccggcctc acagcggcgg ctgcggtggc
     1321 gatggccgca actctgcttc actgccttaa ccactcgtgg tttaagtcac tgctctttct
     1381 cggtgcaggg gcagtgctgc atgccacggg acggcgcgat ttcgacgggc ttggcggatt
     1441 gattcaccgc atgccggtca cagccgcctt ctggctagtt ggcgccatgt cgatttcggc
     1501 cctgccgccg ttcaatggct tcgtttccga gtggttgctg tttcaggccg tgctcgcggg
     1561 gccgcaattt cctgaaccta ttctacgctt catgtctccc gcggttggcg ccatgctggc
     1621 tctggccgct gcccttgccg cggcttgttt cgtacgcgct ttcggcattg tctatcttgg
     1681 ccgtccgcgc agcaatgagg ctgccggagc ccatgaaacg gcacggcccc agcaggtggc
     1741 aatggcgctg ttagccggtc tttgcctgct aggcggatta ttcggcgggg tgatggtttc
     1801 agcaatccag cccttgcttc aaacgctggc cggggcgcct ctcccggcag ttggcacggg
     1861 ccccactcca ctctcactgg tcgcgttcga tgctgcgcgc agcatctacg atgcgccgac
     1921 gattgcaatc ttcctgctga tctcaggtac actgacagtc atgctggtgc accgcatctc
     1981 aagccgccgc accaggcttg gccccgcatg ggattgcggt ttcccggatc cttcaccgct
     2041 cacgcaatac agcgcctcga gcttttcgca gcctttgcgg cgggtctatg gttcgacggt
     2101 ctttggcgct tccgaaacag tcgacatgcc gcaacccggc gacaaccgcg ccgcgcgctt
     2161 cacagtccgg ctgcatgact atgcctggga gctgctttac gccgcaccgg cacgggcagt
     2221 gttgaaaatg tcggaacggc ttaatgccct tcagttcctg acaatccgca cttacctcat
     2281 gctcatgttc agcgcgctca tcgtgctgct tctcattgcg gcggtgtggt tctgatgcgc
     2341 gatacagtca tcctgctgtt cggccagacg atccagatgt tcctggtgct attgattgct
     2401 cccggtatcg cgggcatcac gcggcgcgtc aaggctcgct tcatgcgcag gcagggggct
     2461 cccatcattc agccgtaccg tgacctgatc a
//
LOCUS       DAORVN010000294         2486 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_41237_length_2486_cov_2.557795, whole genome shotgun sequence.
ACCESSION   DAORVN010000294 DAORVN010000000
VERSION     DAORVN010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2486)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2486)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2486
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41237_length_2486_cov_2.557795"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..521
                     /locus_tag="PKV23_06655"
     CDS             <1..521
                     /locus_tag="PKV23_06655"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HQX85151.1"
                     /translation="ALGFKQQGTATVRVQYIRPAPLNDDGSNLMAMNEELERGTPLRQ
                     MIAAAGGKRRSEPVQLAQADPVEEGAYQAEPEQQVIQFEPNIPSPVNYFVQAGTFADP
                     DNAERLRQEFVNIGPVQVAELMGNDGPVYRMRVGPFDNINDAQVALNQVHGYGLPDAH
                     LLESHLQQASLQ"
     gene            583..1749
                     /locus_tag="PKV23_06660"
     CDS             583..1749
                     /locus_tag="PKV23_06660"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HQX85152.1"
                     /translation="MNGIRIVLRTVVLLLLLAASGAAFAQDVLPFETTAPNAILIDAK
                     SGTVFFEKKADELVQPASMSKLMTMVMVFEALKAGTVTLDTEFPISVNAWKRGGTASG
                     GSTMFANVNTKVKLIDLMHGAMIQSANDACIAIAEGMAGSEEEFAARMTLRAKELGLQ
                     DAVFRNVTGLPRPENRASVRDLTILARYVVSEFPEYYKIYGKPEFTWNKITQKNRNPL
                     LLDYPGADGMKTGYIREAGYGLVGSAMRDGRRLILVVAGVSSIAERKEEAQKLLDWGF
                     RQFKTIDVYAAGDVVSKARVWGGTNRWVDLVIRKDLRLALSAREQESIEVKLSYAGPL
                     IAPVKAGAPIGTVKFMMEGKAIAEVQVETANDVAAVDSIWSRAMDSLLILALGG"
     gene            1751..2380
                     /gene="tmk"
                     /locus_tag="PKV23_06665"
     CDS             1751..2380
                     /gene="tmk"
                     /locus_tag="PKV23_06665"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012112039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HQX85153.1"
                     /translation="MPGTFVTFEGGEGTGKSTQLKLLADVIMARGLPLVQTREPGGTP
                     EAEALRTLLVNGDPGRWTAEAEALLNYAARASHLQKIIRPALASGKFVLCDRFSDSTR
                     AYQGYAGGCDLKLLDALEASIVGRTRPALTLILDIDPAMGLARAKSRADGSEDRYERK
                     GLEFHQRLRAGFLQIAAAEPKRCKVIDASGSIGEVTARVLAAVEPVLNG"
BASE COUNT          513 a          695 c          804 g          474 t
ORIGIN      
        1 cggcccttgg cttcaagcag cagggcaccg ccacggtgcg cgtgcaatac attcgcccgg
       61 cgccgctcaa tgacgatggc tcaaacctca tggcaatgaa tgaggagctt gaaaggggaa
      121 cgccgctgcg ccagatgatc gccgcggcgg gcggcaaacg ccgttctgag cctgtgcaac
      181 tggcgcaggc cgatcccgtc gaggaggggg cctatcaggc cgaaccggag cagcaggtca
      241 tccagttcga gccaaacatt cccagtccgg tgaattactt tgtgcaggcc ggaacctttg
      301 ccgatcctga caatgcggaa cggctgcgcc aggaatttgt caacatcggc ccggtgcagg
      361 tcgcagagtt gatgggaaat gacggcccgg tataccgcat gcgcgtgggg ccatttgaca
      421 acatcaatga cgcgcaagtg gcgctcaacc aggttcatgg ctatggcctt ccggatgcgc
      481 acctgcttga atcgcacctt cagcaggctt cgctgcaata gcagcttcgc gttgattttg
      541 ccctgaaagg tcagtaggtt cagcacttca aagaggtgaa gtgtgaacgg catccggatc
      601 gtcctgagaa ctgttgtttt gctgctgtta ttagcagcat caggcgctgc gtttgcccag
      661 gacgtgctgc cgtttgaaac cacggcgccc aacgcaattc tcatcgacgc aaagtccggc
      721 accgtgttct tcgagaagaa ggccgatgaa ctcgtgcagc ccgccagcat gagcaagctg
      781 atgaccatgg tcatggtgtt tgaggccctc aaggccggga ccgtgacgct ggacaccgaa
      841 ttcccaatca gtgtgaatgc ctggaagcgg ggtggcaccg catcgggtgg ttccaccatg
      901 tttgccaacg tgaacaccaa ggtgaagctc atcgacctca tgcacggggc catgatccag
      961 tcggccaatg acgcgtgcat tgcaattgcc gaaggcatgg ctggatcgga agaagaattt
     1021 gcggcgcgca tgactctgcg ggcaaaggag ttgggcctgc aagacgcggt gttcagaaat
     1081 gtgacaggat tgcccagacc ggaaaatcgg gccagtgtgc gcgacctcac tatcctcgcc
     1141 cgctatgtcg tgtcggaatt cccggaatac tacaaaatct acggcaagcc ggaattcacc
     1201 tggaacaaga ttacccagaa gaaccgcaat ccactgctgc tggactatcc aggggctgat
     1261 ggcatgaaaa ccggctatat ccgggaagcg ggctatgggc ttgttggttc tgcgatgcgt
     1321 gatggccgcc gcctgatcct tgttgtggcc ggggtaagtt cgatcgccga gcgcaaggaa
     1381 gaggcgcaga agcttctcga ctgggggttc aggcaattca agacaatcga tgtctatgcg
     1441 gccggcgatg tggtgagcaa ggcacgggtc tggggcggca ccaaccgctg ggtcgacctt
     1501 gtgataagga aagatctgcg cctcgcgctt tctgccaggg agcaggagag cattgaagtg
     1561 aagctctcct atgctggccc gctcatcgct ccggtgaagg caggtgcgcc aatcggcacc
     1621 gtgaagttta tgatggaggg gaaagcgatt gccgaggttc aggtcgaaac ggccaatgac
     1681 gtggcggcgg ttgattcaat ctggagccgc gccatggaca gcctgctgat cctggcgctt
     1741 ggcggctagg atgcctggaa cattcgtcac ctttgagggc ggtgaaggaa ctgggaaatc
     1801 cactcaactg aaactgctgg cggacgtgat catggcgcgg gggcttcccc tcgtccagac
     1861 gcgtgaacct ggcggaacac ccgaggccga ggccttgcgc acccttctgg tcaacggcga
     1921 tcccggccgc tggacggcgg aggcggaggc actcttgaat tatgcggccc gcgcgtcgca
     1981 tttgcagaaa atcatccgcc cggcgctggc ttccggcaag tttgtgctgt gtgaccggtt
     2041 cagcgactcc acccgggcct accagggcta tgcgggcggc tgcgatctga agctccttga
     2101 tgcgcttgag gcctccattg tcggcagaac ccggccggct ttgacgctga tcctggatat
     2161 tgatcctgca atggggcttg cgagggccaa gtcccgggcc gacggatcgg aagaccgtta
     2221 cgagcgcaag gggctggaat tccatcagcg gcttcgcgcc ggattcttgc agattgcggc
     2281 agccgagcca aagcgctgca aggtcattga tgcatccggc agcatcggcg aagtgacagc
     2341 gcgcgtcctt gccgcggtgg agccggtgct caatggctga ggacagcgag gacccgcgtg
     2401 aggtggactg gcacccccgc aaggcaagac gccttctggg acatgcggcg gcggagcagc
     2461 agatgcggca ggccttcgcg tccggc
//
LOCUS       DAORVN010000295         2477 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_41475_length_2477_cov_3.929397, whole genome shotgun sequence.
ACCESSION   DAORVN010000295 DAORVN010000000
VERSION     DAORVN010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2477)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2477)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2477
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41475_length_2477_cov_3.929397"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1351)
                     /locus_tag="PKV23_06670"
     CDS             complement(<1..1351)
                     /locus_tag="PKV23_06670"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HQX85154.1"
                     /translation="MDLQLPRGIAVIDAGKTNTKIVLFGPDGRPVAERKVASLNHDGP
                     PYRAIDPAPLIALCRSALPELDAILPIDTVVTSAHGAALACLGEDGSLALPVMDYNAE
                     PPPEIIAAYRKVQPPFDEVYSSLLPMALIHGLQLFWQQQAFPRDFARVKTVVPWIQYV
                     AYCLSGKLVTEISGMACQSHLLNIRDGGYSSLVKRMGWEPLFPPMAKAWETIGTLRGE
                     FLGNGFRGRGNVLAGVHDSNANYLRYLAGGQKKFTLLSTGTWIIGFDTETGLASLIEE
                     RDTVSNTDVFGNRVGSGRFFGGREFEIVSGGASGDMASLPAVQDLIATTTYALPSFSD
                     GGGPIPRTGGRGRIVGTPPLTPEQKASLASLYCALMVAECLAALGSKHDIIVDGPFSR
                     NLVFLQVLAQLRQGQRVLASDLRDGTTAGAACLALMPDGVLPHVPLALTTIASQNLNN
                     "
     gene            1435..2154
                     /locus_tag="PKV23_06675"
     CDS             1435..2154
                     /locus_tag="PKV23_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018095511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HQX85155.1"
                     /translation="MKHLDLRREVIETCLKMNADGINQGTSGNVSVRCDEGFLITASG
                     IVYDKMKPAHVIEMDLEGNYRGDYLPSTEWRMHMDIFRARPEAKAIVHVHSTYATALA
                     CLRREVPAFHYMIGVTGGTSLRVSDYAEFGTQELSDTMLKAMEGRTGCLLANHGQICF
                     GPSLAKALWLAGEIEHLCHQYWAAGLAGKPVILDEAQMASVLKRFKTYGKQAGDMVKG
                     DAPAVLAPRLMPSKSKKKGKS"
     gene            2151..>2477
                     /locus_tag="PKV23_06680"
     CDS             2151..>2477
                     /locus_tag="PKV23_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006330173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HQX85156.1"
                     /translation="MIANVSAFVNELNARAETGKPVTIGLAGAGQMGTDIVVQLSLMP
                     GLRLGAISEVNTSRAVDAALLAGHGRERLAMTKSAGEIDSAIERGRLAITDDLSALVA
                     AGRIDVV"
BASE COUNT          529 a          700 c          769 g          479 t
ORIGIN      
        1 gattgttgag gttttgcgac gcaatcgtgg tgagcgccag cggcacatgg ggcagaacgc
       61 catccggcat cagggccaag caggcggccc ccgccgtagt cccatcgcgc aggtctgacg
      121 ccagcactct ttgtccctgc cgcaattggg ccaggacctg caggaaaacc aggttccttg
      181 aaaatggccc atcgacgatg atgtcatgct tcgatcccag ggccgcgagg cactccgcca
      241 ccatgagggc gcaataaagc gaggcaaggg aggctttttg ttccggcgtc aaaggcggag
      301 ttccgacaat cctgccgcgt ccgccggtgc ggggaatcgg cccgccgcca tcggagaagc
      361 tcggcagggc ataggtcgtg gttgcaatga gatcctgcac ggccggcaag gacgccatat
      421 ctcctgatgc gccgccgctc acgatctcaa attcgcggcc gccaaaaaac cggccgctgc
      481 ctacgcggtt tccgaataca tcggtattcg atacggtatc cctctcttcg atcaggctgg
      541 caagcccggt ttccgtgtca aagccgatga tccaggtgcc ggttgaaagc agggtgaatt
      601 tcttctgccc gcccgcgaga taacggagat aattcgcatt ggagtcatga acccccgcca
      661 gaacgttgcc gcggccgcga aagccatttc ccaagaattc accgcgcaag gtgccaatcg
      721 tttcccaggc cttggccatg ggcggaaaca agggttccca tcccatgcgt ttcaccaggg
      781 acgaatagcc gccgtcgcgg atgttcagga gatgggactg gcaagccatg ccggaaatct
      841 cggtgaccag ttttccgctc aggcaatagg ccacgtattg gatccacggc acaaccgtct
      901 tcacccgcgc aaaatcccgg gggaatgcct gctgctgcca gaagagttgc aggccatgga
      961 tcagggccat gggaagcagc gaggagtata cctcatcaaa gggcggctgc accttgcggt
     1021 aggccgcgat gatttccggc ggcggctcgg cgttgtagtc catgaccggc agggccagag
     1081 aaccatcctc gcccaagcag gcaagggccg cgccatgggc ggaggtcacc accgtatcaa
     1141 tgggcaaaat ggcgtcgagt tcaggaaggg cgctgcggca gagtgcgatg agcggggccg
     1201 gatcgatggc gcggtaaggc gggccatcat ggttaaggct ggcaaccttg cgctccgcaa
     1261 ccggcctgcc gtcaggccca aacaggacga ttttggtgtt ggtctttccg gcgtcaataa
     1321 cggcaattcc acgcggcaat tgtaaatcca tctgggcttt cctatgatgc ccgcgacact
     1381 gttaaatcaa cgccttgaag tcaatccttc acaactggaa atgaaatgaa aaagctgaag
     1441 catctcgacc tgcgccgcga ggtcatagag acctgcctga aaatgaatgc ggacggaatc
     1501 aaccagggaa cctccggcaa cgtcagcgtg cgttgcgatg aaggatttct cattaccgct
     1561 tcgggaattg tttatgacaa gatgaagccc gctcatgtca ttgaaatgga ccttgagggc
     1621 aattatcgcg gggactatct gccctcgact gaatggcgga tgcatatgga tatcttccgg
     1681 gcgcggcctg aagccaaggc catcgtgcat gtgcattcaa cctatgcaac ggcactcgcc
     1741 tgcctgcgcc gggaagtgcc ggcgttccac tacatgatcg gggtcaccgg cggcacgtcc
     1801 ctgcgcgtgt ccgattatgc ggagtttggc acccaggaac tttccgatac catgctgaaa
     1861 gcaatggaag ggcgcacggg ctgcctgctt gccaaccatg ggcagatttg ttttgggcca
     1921 agcctggcaa aggccctgtg gctggcgggc gaaattgagc acctatgcca ccagtattgg
     1981 gcggcgggcc tggcgggcaa gccggtcatt ctggatgagg cccagatggc gagtgttctc
     2041 aagcgtttca aaacctatgg caagcaagcc ggcgacatgg tgaaaggcga tgcccctgcg
     2101 gttcttgcgc cgcggctgat gccttccaag agcaagaaga aaggcaagtc atgattgcca
     2161 atgtttcggc gttcgtcaac gaactgaatg cgcgcgccga aacgggaaaa cctgtcacca
     2221 tcggccttgc gggagcaggc cagatgggaa ccgacatcgt tgtgcaactg tcgctgatgc
     2281 cgggtttgcg gcttggggca atatcggagg tgaatacctc gcgtgcggtt gatgcggccc
     2341 ttcttgccgg ccatgggcgc gagcggcttg ccatgaccaa gtcggcgggc gagattgaca
     2401 gcgccattga acgtgggcgg ctggccatca ctgatgatct ttccgcattg gttgcggcgg
     2461 ggcgcattga tgtagtg
//
LOCUS       DAORVN010000296         2477 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_41483_length_2477_cov_2.872419, whole genome shotgun sequence.
ACCESSION   DAORVN010000296 DAORVN010000000
VERSION     DAORVN010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2477)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2477)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2477
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41483_length_2477_cov_2.872419"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(157..915)
                     /locus_tag="PKV23_06685"
     CDS             complement(157..915)
                     /locus_tag="PKV23_06685"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HQX85157.1"
                     /translation="MNKTMERSTFLTIAHRGASAYAPENTFAAFELALQMGACHIELD
                     VRVTADGQAVVIHDETLDRTTSGAGPVAQRSLAELKEFDAGSWFDSRFASEQVPAFTE
                     VLERYAKLAHLHTEIKSGQVGFCKHIIDEIHQHGAEQRVTITAFELAHLVEVRRLAPN
                     LSTGWLVRNITEELMATAKRMGIDQLCPQASTVSPEIVRLLHNEGFTVRAWGVPSEAV
                     MRAVVRSGADGMTVNFPDKLIHYLAMGEKGSTPI"
     gene            1494..>2477
                     /locus_tag="PKV23_06690"
     CDS             1494..>2477
                     /locus_tag="PKV23_06690"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HQX85158.1"
                     /translation="MPTSHGISRREFLRTASLLAAGGVLMACAPATVAPAASGPTATP
                     APFVKGDIRIVPSGVQLPTEPVTFRWLDSGDTKAVFWKQFFAKYQEAHPNITVQYDGL
                     PWNEIGKIVPLGIQNGNLHDVFSIPQGITSAQAYAEGWLMPYDDLLSDLAAVKAAYPV
                     GSFVEGINVFGGKTYCLPHSTSKITSTMLLFNQDYLQEAGYDPAANTMTWDDYRVAAK
                     KVTENGKGQYYGVIIGGNQLNRWGAVVRDLATFAGVPNVGSTLDDMNLLTGDYNFTSD
                     EYIAAMELLLAMNSDGSFFPGYLSINAPQARAQIAQGAAGMILQGLWCLPQW"
BASE COUNT          512 a          743 c          693 g          529 t
ORIGIN      
        1 aggcccgccg gggaaggggt atagcgcggt gggaatgcag agggcgctgg cctcggcaat
       61 cggtttgccc agcaggtcga ccagcgcgtg gcgaatgctc gtgttcgtga tgcccgcgga
      121 agtgagtaag aatttcataa tgcttctcct tgccgattaa atgggagtag atcctttctc
      181 tcccatggcc agatagtgaa tgagtttgtc tgggaaattg accgtcattc cgtccgcgcc
      241 tgaccgcacc acggctcgca tcactgcttc ggatggcacg ccccatgccc gcaccgtgaa
      301 gccttcattg tgcagaagcc gcacgatctc cggggacacc gtgctcgcct ggggacacaa
      361 ctggtcgatg cccatacgct ttgccgttgc catcagctcc tcggtgatgt tccgcaccag
      421 ccaaccggtt gacagattgg gggcgagtcg gcgaacttcc accaaatgcg ccaactcaaa
      481 cgccgttatg gttacacgct gctccgcgcc atgttgatgg atctcatcga taatatgttt
      541 gcagaaccca acctgtccgc tcttgatctc cgtatgcagg tgcgccagtt tggcatagcg
      601 ttcgagaact tccgtgaatg cgggaacctg ttcactggca aatcgtgagt caaaccacga
      661 accggcatca aactccttaa gctcggccag ggaacgctgg gccacggggc cggcgccgct
      721 ggtggtcctg tcaagggtct cgtcgtgaat gaccacagcc tgcccatccg cggtgacacg
      781 aacatccaat tcgatgtgac aagcgcccat ctgcaaggca agttcaaagg ccgcaaaagt
      841 attttccggc gcataggcag aagccccccg gtgggcaatg gtcaaaaatg tcgatctttc
      901 catcgttttg ttcatcctgc taatgtcccg ctcacctgcg ggcgtcagcc cgccaggtgc
      961 agcgggtagt ttggcggcgg tagccgcccc cgctgcatcg gtgagcggga cattttgcgg
     1021 gcggcgtagc cgtccgcaaa actactcatt atcgagcaaa tagatggtga tttctcctgt
     1081 ccgggatcat atcgtgctca ttggacgata tgatccatca tgcggctgat tatcaatcag
     1141 atcggcaaaa tcatcaactg atttctctga acaaacagct catcacccgc aacttgcccc
     1201 gctgcgctgc acatccacca ccaagccggc ccgcaagcgc gcaaacccac ccacaacgaa
     1261 caacaggcca aaaaccatgc gcttgaccag ctttctgaag agtcttcctg gagttggctt
     1321 tccaaaaagt tgcgcgaatc gcgcgttttt cttcctgcta cttttttgtt agactacgga
     1381 cgtcgcactg actgtagcga aattcttccc aatctgtcta ccgatccggc gatttccgcc
     1441 ggattcgccc atccagactt tgttcactgt gactgaggaa aggagctccc aggatgccaa
     1501 cttctcatgg aatttcgcgt cgcgagtttt tacgcacggc gtcgctactg gccgccggcg
     1561 gcgtgttgat ggcctgtgcg cccgccaccg tggcgccggc cgccagcggg ccgaccgcga
     1621 cgcccgcgcc ctttgtcaag ggcgatatcc gcatcgtgcc cagcggcgtg cagttgccga
     1681 ccgagccggt caccttccgt tggctggaca gcggcgacac caaggcggtc ttctggaagc
     1741 agttctttgc caaatatcag gaagcgcacc ccaacatcac cgtgcaatat gacggcctgc
     1801 cctggaacga gatcggcaag atcgtgccgc tgggcattca gaacggcaac ctgcacgatg
     1861 tcttcagcat tccgcaaggc atcaccagtg cacaggcgta tgctgaggga tggctcatgc
     1921 cttacgacga tctgctatct gatctggccg cagtcaaagc tgcttatcct gtcggctcct
     1981 ttgtcgaggg catcaacgta ttcggtggca aaacctactg tctgccgcat tcgacgagta
     2041 aaattacgtc gactatgctg ctcttcaacc aagattatct gcaggaggcg ggctacgatc
     2101 cggccgctaa taccatgacg tgggacgatt accgcgtcgc ggccaagaag gtgacggaaa
     2161 atggcaaggg tcagtactac ggcgtcatca tcggcggcaa ccagctcaac cgctggggcg
     2221 cggtggtgcg tgatctggca acctttgccg gcgtccccaa cgtgggcagc acgctggacg
     2281 atatgaacct gctcaccggc gactacaact tcacctcgga tgagtacatc gcggcgatgg
     2341 agctgttgtt ggcgatgaac agcgacggca gcttcttccc aggctatctt tcgatcaatg
     2401 cgccgcaggc gcgtgcccag atagcgcagg gcgcggccgg tatgattctg caagggctgt
     2461 ggtgtctgcc gcagtgg
//
LOCUS       DAORVN010000297         2474 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_41564_length_2474_cov_3.384043, whole genome shotgun sequence.
ACCESSION   DAORVN010000297 DAORVN010000000
VERSION     DAORVN010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41564_length_2474_cov_3.384043"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..379)
                     /locus_tag="PKV23_06695"
     CDS             complement(<1..379)
                     /locus_tag="PKV23_06695"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HQX85159.1"
                     /translation="MSVRADAETLRQIPIFAECEPVHLQLLAFASERQHFVSGEAIIS
                     EGKKAKSAFLLLSGNADIQQTTGLHRQSIARAEPGSFLGEVAMIGRTTYAITALAMGP
                     VATARIDHELFLRVANEYPEFGQS"
     gene            complement(467..1153)
                     /locus_tag="PKV23_06700"
     CDS             complement(467..1153)
                     /locus_tag="PKV23_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HQX85160.1"
                     /translation="MTNTKKILIVDDDDTLRDTLKDQFSLHDEYTVTDVATATAGVKA
                     VKADHADMVILDVNLPDMDGREACKLMRRNGYKGPVIMLTGQSSDSDTILGLDSGAND
                     YITKPFRFGVLLARIRAHLRQHEHSEDATFKVGPYTFKPASKILVRDDNKKVRLTEKE
                     TAIIKFLYRSGEQIVSRDVLLHDVWGYNAGVTTHTLETHIYRLRQKIERDPSQAEILV
                     TEGGGYKLVP"
     gene            1240..1803
                     /locus_tag="PKV23_06705"
     CDS             1240..1803
                     /locus_tag="PKV23_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009490644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HQX85161.1"
                     /translation="MSKTVTYLHVRAISARATRGVLTCGSFVVPCALGRSGRIVLKRE
                     GDGGSPVGIWLLRRIYYRGDRFIRPLSKIPASPIRGGWGWCETVGDRNYNRRVDLPYP
                     TAHEDLKRPDHLYDIVVETSHNQRPRIQGRGSAIFFHLARESFVPTAGCIAVTLKDMQ
                     KVLRHCSAKTRLVIWPPSGIVPPVFRK"
     gene            complement(1769..2449)
                     /locus_tag="PKV23_06710"
     CDS             complement(1769..2449)
                     /locus_tag="PKV23_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009490649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HQX85162.1"
                     /translation="MNPPQHKALQEVRKRIESAAQSAGRKAGEITLIAVSKTFGAEDI
                     LPVLQAGQTVFGENRVQETAGKWPNIRATAPGLELHLIGPLQSNKAADAVRLFDAIHT
                     IDRPRIAQAVGAEMARQARQLELFIQVNTGDENQKAGVSVASADSFIDDCRTKYGLQI
                     SGLMCIPPINDVPDKHFELLATIARRNDLKHLSMGMSGDFEAAINLGATHVRVGSAIF
                     GTRAGQSR"
BASE COUNT          485 a          731 c          701 g          557 t
ORIGIN      
        1 cgctttggcc aaattccgga tattcattgg caacccgcag gaaaagctca tggtctatcc
       61 ttgcggtggc aaccgggccc atggccagtg cagtgatggc ataggttgtt cttccaatca
      121 tcgccacttc gccgagaaag gacccgggtt ccgcccgtgc aatggattgc ctgtgcaggc
      181 ctgttgtttg ctggatgtcg gcatttccgc tcagcagcag gaaagccgat ttggcctttt
      241 tgccctccga gataatggct tccccggaga cgaagtgctg gcgctcagag gcaaaggcga
      301 ggagttgcag gtgcacgggt tcgcattcgg caaagatcgg tatttgccgc aaggtctcgg
      361 catctgcgcg gacactcatc tcacaagccc caagcgggcc ccaaagccca tgattcccaa
      421 ggatttccca ttgccagcgc cattgaacga tggataggcc ccaagatcaa gggacaagct
      481 tatagccgcc gccctcggtg accaggattt ccgcctggct cggatcgcgc tcaattttct
      541 gccgcaggcg atagatgtgc gtttccagcg tatgggtcgt gacccccgca ttgtagcccc
      601 agacatcatg cagcagcacg tcacggctga cgatctgttc gccggaacgg taaagaaact
      661 tgatgatcgc cgtctccttt tcggtgaggc ggactttctt gttatcgtcc ctcacaagaa
      721 ttttggaggc gggcttgaaa gtgtaggggc cgaccttgaa cgtggcgtct tcgctgtgct
      781 cgtgctgccg cagatgcgcc ctgatgcgcg ccagcagcac gccaaaccgg aatggcttgg
      841 tgatgtaatc atttgcgccc gaatcgaggc ccaggatggt atcggaatca gaactctgtc
      901 cggtcagcat gatgaccggg cccttgtagc cgttgcgccg catcagcttg caggcttccc
      961 ggccatccat gtcaggcagg ttcacatcaa ggataaccat gtctgcgtga tcggctttga
     1021 cagccttgac accggccgtt gcggtggcaa catccgtgac cgtgtattca tcatgcaggg
     1081 aaaactggtc cttcaacgtg tcgcgcaggg tatcgtcgtc gtcgacgatg agaatctttt
     1141 tggtgttggt cattgcggat ctctcatcac attcagggca tgggacaatt ctagagcgat
     1201 caatgacctt ttgatagccc gggggcaaga caactgaccg tgagcaaaac cgtcacatat
     1261 cttcacgttc gcgcgatcag cgcgcgcgcc acccgtggcg tgctgacatg tggttccttt
     1321 gtggtcccct gcgccctggg acgatccggc agaattgtcc tgaagcggga aggtgacggc
     1381 ggaagtccgg ttggcatctg gcttctgcgc cggatttact accgcggaga ccgcttcatc
     1441 cgtcccctgt caaaaatccc tgcatcgccg atcaggggtg gctggggctg gtgtgaaacc
     1501 gtgggggaca ggaattacaa tcgccgggtg gacttgccct atcccaccgc ccacgaggac
     1561 ctgaagcgcc ccgaccatct ctatgatatt gtggtggaga ccagccacaa ccagcggccc
     1621 cgcattcagg gccgcggcag cgccatattt tttcatcttg caagggagag ttttgtcccc
     1681 accgccggct gcattgcggt aaccctcaag gacatgcaga aagtgctgcg ccactgctcg
     1741 gccaaaaccc gcctggtgat ctggccgcct agcgggattg tcccgcccgt gttccgaaaa
     1801 tagccgaacc gacccgcaca tgggtggcgc caaggttgat cgctgcctcg aagtctccgc
     1861 tcatgcccat gctcaggtgc ttaagatcat tgcgccttgc gatggtggcc agaagctcaa
     1921 aatgcttgtc tggcacgtca ttgatgggcg gaatgcacat gaggccagaa atttgcaaac
     1981 catatttggt ccggcagtca tcgatgaagc tatccgcact ggcaacgctg acacccgcct
     2041 tctgattttc atcacccgta ttgacctgaa tgaaaagctc cagctgacgt gcctgccttg
     2101 ccatttccgc gccaaccgcc tgggcaatgc gcggccggtc aatcgtatgg atggcatcaa
     2161 acagacggac tgcgtccgcc gctttgttgc tttgcaacgg accaattaga tgcagctcca
     2221 gcccgggagc tgtcgccctg atatttggcc actttcctgc cgtttcctga accctgtttt
     2281 ccccaaagac cgtctgcccg gcttgcagga ccggcaagat gtcttccgcc ccgaaggtct
     2341 tgctcaccgc gatcagcgta atctcgccag ccttgcggcc agcagactgg gcggcgcttt
     2401 caatgcgctt tcggacctct tgcagggcct tgtgctgcgg tggattcatg ctgtgcttat
     2461 ggccaagctt gcag
//
LOCUS       DAORVN010000298         2471 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_41690_length_2471_cov_2.056705, whole genome shotgun sequence.
ACCESSION   DAORVN010000298 DAORVN010000000
VERSION     DAORVN010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2471)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2471)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2471
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41690_length_2471_cov_2.056705"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="PKV23_06715"
     CDS             <1..314
                     /locus_tag="PKV23_06715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85163.1"
                     /translation="NISGRQDQFLRFPEGIRSATGLRQALLRENLDALAFQIAQVETF
                     KLEIRFVPARVKKDINPDPIVTHIRQLIHPKLEVVFKPVEKVPLNPGGKLQRIVCELA
                     S"
     gene            311..1381
                     /locus_tag="PKV23_06720"
     CDS             311..1381
                     /locus_tag="PKV23_06720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85164.1"
                     /translation="MNALHKQIFDMLMESQYWTPAQMLAFHRHQLAHLLRHARQYVPF
                     YSTRLDSVFRKDGEIDWDRWQEIPITKRHHLVEERESMLATKLPPNHGGVWEEFSSGS
                     SGSPVTTRHNLLEGWVSAAVLHRTQRWHNLDWSRTFVSWSGNDGLISNWPDGIELESW
                     APSWLEPNHRGRSYKVNRATSQENVIEYVLRKNAPYLASRPKLLQSLAFATERLKTPL
                     RVDVVSTFGTGITEDERDDIRRILGAKVLSLYSSGEGCKIAATCESGHHYHVNPELNY
                     LEILDDKGQPCAMGQPGRVIITPIYNTAQPLIRYEQGDIAVRGPACSCGKQLPVLQEI
                     SGRVIHLFRFPDGQIIAPSLPN"
     gene            complement(1631..>2471)
                     /locus_tag="PKV23_06725"
     CDS             complement(1631..>2471)
                     /locus_tag="PKV23_06725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85165.1"
                     /translation="YAPGLSLLARGRWPVLAVGNGYTMPPPEMAAFRPLAKLNRPLYA
                     TEPEIIDRLNKDLKKIGAQSIERLPQLNEADGYGLMTIPVFDPYRAQRQQPYLGAQVP
                     GGMPTPRPAASGGIAYFHEDTQLDDNVLNGLVGANINMLAYFGKHLRRVVKKFSGSRV
                     SLADMPFNLHKDLPGKTVAVHNGGLGFATAAILAGIPQVMLYRHEEHWFMANAVVAAG
                     AGAAAQYKDVTSRALSEAIDRVSKSTAMRESAMRLAEEHAGFQNANPARAIAEIATRL
                     LSR"
BASE COUNT          542 a          701 c          664 g          564 t
ORIGIN      
        1 gtaacatctc aggtcggcag gaccagtttc tgaggtttcc cgaaggcata cgctctgcga
       61 caggactgcg gcaggctttg ttgcgtgaga acctggatgc cctggccttc cagatcgcgc
      121 aagtcgagac cttcaagctg gaaattcgct ttgttccagc tcgcgtgaaa aaggacataa
      181 atcctgaccc tattgtcacc catatccggc agcttattca tccaaaactg gaagtggttt
      241 tcaagcccgt cgagaaagtc ccgctcaatc ccggaggaaa gcttcagcgc atcgtttgtg
      301 agcttgcctc atgaatgccc tccacaagca gattttcgat atgctcatgg agagccagta
      361 ttggacgccc gcgcaaatgc tggcgttcca ccgccatcag ctggcacatc tgctccgcca
      421 tgccaggcaa tacgtcccct tctacagtac ccgtctagat tccgttttcc gcaaggatgg
      481 cgagattgat tgggatcgct ggcaggaaat tccgatcaca aagcgccacc atcttgttga
      541 agagcgcgaa agcatgctgg cgaccaagct gcctcccaac catggcggtg tatgggaaga
      601 attcagctct ggctcatcag ggagtccagt tacgaccagg cacaatcttc tcgaaggctg
      661 ggtttctgca gcggtcctgc accgcacgca gcgctggcac aatctggact ggtccaggac
      721 attcgtaagc tggagcggta atgatggcct gatctccaac tggccggacg gtatagagct
      781 tgagtcatgg gcgccaagct ggctggaacc aaatcatcgc ggccggagct acaaggtcaa
      841 tcgtgcgacc tcgcaggaaa atgtcattga atatgtgctg cgcaagaacg ctccatatct
      901 tgcaagccgt cccaaacttc tgcaatccct ggcatttgca acagagcgcc tgaaaacacc
      961 gttacgcgtc gacgtcgttt cgacttttgg caccgggatt acggaggacg agcgcgacga
     1021 tatccggcgc attctgggtg cgaaggtgtt gtcactctac agttcgggcg aagggtgtaa
     1081 aattgccgcc acgtgtgaat caggccacca ctatcacgtc aatccggagc tcaactatct
     1141 ggaaatcctg gacgacaagg gccagccatg cgccatgggc cagcccggtc gcgtcatcat
     1201 cacacctatt tataacaccg cccagccctt gatccgctac gaacagggcg acattgccgt
     1261 tcgcggcccg gcctgttcct gtggaaagca attgcccgtt ttgcaggaaa tatcaggccg
     1321 tgtgatccac ctctttcgct tcccggatgg ccagattatt gcaccttcct tgcccaacta
     1381 ggagttcgtc gataatttcg gggccaagac ttggcagctt gtgcaaaccg ggcccctgga
     1441 agttgacttg cgttacgtga cgaccgatcc aggtttcgtt cccaataagg cttatgcact
     1501 ggaagcaatc cggcgcaggc ttcgacccga tctccaggtc aatttcgttg ctctggccga
     1561 gactcccttg actgcggcag gaaagttcat cctgtacaaa tcggaacttg ccaatcgcga
     1621 ttgaggattg tcacctgctc aacagccttg ttgcgatctc ggcaattgcg cgtgcaggat
     1681 tggcgttttg aaaaccggca tgttcttcag caagccgcat tgcgctctcg cgcatggccg
     1741 tagatttgct gacgcgatct atcgcttcag aaagtgcgcg ggaggtaacg tctttgtact
     1801 gggctgctgc tccggcgccc gccgcgacaa cggcgtttgc cataaaccag tgttcctcat
     1861 gtctgtacag catgacttgt ggaatccccg ctaaaatggc cgctgtggca aaccccaacc
     1921 caccgttgtg aacggcaacg gttttgccgg gcaagtcttt atgaagattg aagggcatat
     1981 ccgcgagcga gacacgcgag ccactgaatt ttttcaccac tcggcgcaga tgcttgccaa
     2041 aatatgccag catattgata tttgccccca caagaccatt gaggacattg tcgtcgagct
     2101 gcgtgtcttc atgaaagtat gcaatgccgc cagacgccgc cggtctcggg gtcggcatgc
     2161 cgccaggcac ctgtgcgccc aggtaaggct gttgtcgttg cgctctgtag ggatcaaaca
     2221 ccggaattgt catgaggcca taaccatcag cttcgttcaa ttgcggcagc cgttcgatgg
     2281 actgggctcc gatcttttta aggtctttgt tcagcctgtc gattatttcc ggctcagtgg
     2341 cgtaaagtgg gcggttcagt ttcgccaacg gccggaaggc cgccatttca ggcggaggca
     2401 tggtgtagcc atttcccact gccaatacag gccatctacc acgcgccaaa aggctgaggc
     2461 ctggggcata a
//
LOCUS       DAORVN010000299         2468 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_41772_length_2468_cov_3.073353, whole genome shotgun sequence.
ACCESSION   DAORVN010000299 DAORVN010000000
VERSION     DAORVN010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2468
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41772_length_2468_cov_3.073353"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..432)
                     /locus_tag="PKV23_06730"
     CDS             complement(<1..432)
                     /locus_tag="PKV23_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008872827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HQX85166.1"
                     /translation="MGKTIRLTTAQALVKFLTRQMTLVDGKKLPVFAGVWAIFGHGNV
                     VGIGEALYGARKELPTYRAHNEQAMAHAAIAFAKGSRRRRMMACTSSIGPGALNMVTA
                     AAVAHVNRVPVLLLPGDVFANRLPDPVLQQIEDFGDGTISVN"
     gene            complement(445..2361)
                     /gene="iolC"
                     /locus_tag="PKV23_06735"
     CDS             complement(445..2361)
                     /gene="iolC"
                     /locus_tag="PKV23_06735"
                     /EC_number="2.7.1.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003527786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047590 - 5-dehydro-2-deoxygluconokinase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-2-deoxygluconokinase"
                     /protein_id="HQX85167.1"
                     /translation="MASGTGSRAALDVITVGRCSVDLYGQQIGSRLEDIASFAKSVGG
                     CPSNIAIGTARLGLKSAVITRVGDEQMGRYVREQMAREGVSTQGITTDPQRLTSLVLL
                     SVENDKTFPLIFYRDNCADSALCEADIDEKFVASSTALLVTGTHFARPLNDAAQRKAM
                     KIAKANGRKICFDIDYRPNLWGLAGHGAGEERYIKSDKVSEHIKTVLPDCDLIVGTEE
                     EVHIAAGSEDTLKALKTIRSLSSATIVLKRGPMGCVVYPGTIPNRLEDGIVGQGFPIE
                     VYNVLGAGDAFFSGFLRGWLKDEPLETCATYANACGAFAVSRLLCSPEIPTWDELQYF
                     LKHGSPHKALRKDEAINHVHWATTRREQAPKLLALAIDHRIQLEAIADKVGAPRERIA
                     PFKRLAIAAIGKVASGKPGFGTLLDDTYGRDAMFEAAKLGLWVARPLEQPGSRPLRFE
                     FTQDVGARLVEWPVTHTIKCLAFYHPDDPAELKAEQAQKLRTLYEAARKIGRELLVEI
                     IAGKHGSVTDDTVSRALADLYALGIKPDWWKLEPQATGNAWGNIEKVIKTNDPYCRGI
                     VLLGLDAPEEELARAFATTAGHSAVKGFAVGRTIFSSVAESWLAGKIDDDKATSDMAE
                     RFANLVNVWNKSHP"
BASE COUNT          480 a          691 c          772 g          525 t
ORIGIN      
        1 attcaccgaa atggtgccgt cgccaaaatc ctcaatctgc tgcaacacgg gatcgggcag
       61 gcggttggcg aagacatcgc ccgggagcag cagtaccgga acgcgattga catgtgcaac
      121 ggcggcggcg gtcaccatgt tcaaggcgcc gggaccgatg gaggaggtgc aggccatcat
      181 gcggcggcgg cgcgagccct tggcaaaggc aatggccgca tgggccattg cctgttcgtt
      241 gtgggcgcgg taggtcggca gttccttgcg tgcgccataa agcgcctcgc caattcccac
      301 aacgtttcca tggccgaaaa tggcccagac gccggcaaaa accgggagct ttttgccatc
      361 caccagggtc atctggcggg tcagaaactt caccagggct tgcgcggtgg tcaggcggat
      421 tgtcttgccc atgatctatc ctcgttaagg gtgcgattta ttccagacat ttaccagatt
      481 ggcaaagcgt tccgccatgt ctgatgtcgc tttatcgtcg tcaattttcc cggccagcca
      541 gctttccgct acgctgctga aaatcgtgcg gcccacggca aagcccttga ccgctgaatg
      601 accggcggtg gttgcaaagg cacgggccaa ttcttcctcg ggcgcatcaa gtccaagcag
      661 gacaatgcca cggcaatagg ggtcgttggt cttgatgact ttttcgatat tgccccaagc
      721 gttgccagtc gcttgcggct ccagtttcca ccagtcaggc ttgatgccaa gggcatagag
      781 atcggccaag gcgcgcgaaa ccgtatcgtc cgtcacgctg ccatgcttgc cggcgatgat
      841 ttcaaccagc agttcgcggc cgatttttcg ggcggcttca taaagggtgc ggagtttttg
      901 cgcctgctcc gccttgagtt cagcgggatc atcgggatga tagaaggcca ggcactttat
      961 ggtgtgggtg accggccatt ccaccagcct tgcgcccacg tcctgggtga attcaaatcg
     1021 gagcggccgc gagcccggct gttcaagggg gcgcgccacc cagaggccga gctttgctgc
     1081 ttcaaacatg gcatcccggc cataggtatc atcgagaagc gttccgaagc ccggcttgcc
     1141 gcttgccact ttgccgatgg cggcaatggc caggcgcttg aatggggcaa tgcgttcgcg
     1201 aggtgcgcct actttgtcgg caatggcttc gagctggatg cggtgatcga tggcgagcgc
     1261 cagaagtttt ggcgcctgtt cacgccgggt cgtcgcccaa tgcacatgat tgatggcttc
     1321 gtccttgcgc aaggccttgt gcgggctgcc gtgcttgaga aaatactgca gttcatccca
     1381 ggttggaatt tcgggcgagc acaggagcct tgagaccgcg aaggccccgc aggcattggc
     1441 ataggtggcg caggtttcaa gtggttcatc tttcaaccag ccgcgcagga agccggagaa
     1501 gaaagcgtcg ccggcgccaa gcacattgta aacctcgatg ggaaagccct ggcccacgat
     1561 gccgtcttcc aggcggttgg gaatggtgcc gggatagact acgcagccca tgggcccgcg
     1621 cttcaggacg atggttgccg aagaaaggga cctgatcgtt ttcagcgctt tcaaagtgtc
     1681 ctcactgccg gcggcaatgt ggacctcttc ttccgtgcca acgatcaggt cacagtcagg
     1741 gagaaccgtc ttgatatgct ccgatacctt gtcggacttg atgtagcgct cttcaccggc
     1801 gccgtggccg gcaaggcccc agaggttggg gcggtaatca atgtcgaaac agatcttgcg
     1861 gccattcgcc ttggcgattt tcatggcctt gcgctgggcc gcgtcattca aaggcctggc
     1921 aaaatgagtg ccggtcacta gcagggcggt ggaggaagcc acgaattttt cgtcgatatc
     1981 ggcctcgcag agcgcactgt cggcgcagtt gtcgcggtag aagattaatg ggaatgtctt
     2041 gtcgttttca accgagagca ggaccagcga ggtcagccgt tgcgggtcgg tggtgattcc
     2101 ctgggttgaa acgccttcgc gcgccatctg ctcgcgcaca tagcgcccca tctgctcgtc
     2161 gccgacgcgg gtgatcaccg cggatttgag gccgaggcgg gcggtgccga tagcgatatt
     2221 ggaggggcag ccgcccaccg acttggcaaa ggaggcgatg tcctcgagcc gtgagccgat
     2281 ctgttggccg tagagatcga cggagcagcg ccccacggtg atgacgtcaa gggcggctct
     2341 gctgccggtt ccagacgcca tttctgcctc ctcaggattg tgttagcttg acaagacgaa
     2401 acaacaattc caaaatgaag tcaagttgga atgtttgttc tgttttgctc aaggaggcct
     2461 cctcagtg
//
LOCUS       DAORVN010000300         2467 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_41817_length_2467_cov_2.795605, whole genome shotgun sequence.
ACCESSION   DAORVN010000300 DAORVN010000000
VERSION     DAORVN010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2467)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2467)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2467
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41817_length_2467_cov_2.795605"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..879)
                     /locus_tag="PKV23_06740"
     CDS             complement(<1..879)
                     /locus_tag="PKV23_06740"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014893003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HQX85168.1"
                     /translation="MALRQIPEYLTFDDVLMKPGASSVLPAEVSTATHITRDIQLSIP
                     IISAAMDTVTESKLAIAMAQAGGLGVIHKNLEPDVQAEHVRQVKRFESGMVVNPITIR
                     PEATLAEVMQLKDRYRISGIPVVEQNGKLAGIITNRDVRFATDMNTKVSDLMTRNLIT
                     VKAGVDRLEAKRLLHKHRIEKLIVVNEDYECVGLITVNDMEKAADHPFAAKDEQERLR
                     VAAATGTGEKGFLRAEMLIDAGVDIIVVDTAHGHSIHVINQVNRIKKLSNKVQIVAGN
                     VATGEAVKALIGAGADS"
     gene            1021..1947
                     /locus_tag="PKV23_06745"
     CDS             1021..1947
                     /locus_tag="PKV23_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HQX85169.1"
                     /translation="MGHAHDHDLHAPGHVHAPASFGKAFAIGIALNLAFVLIEAGYGI
                     VANSMALLADAGHNLSDVLGLVVAWLAAVLSNRPPSRRYTFGLKSSTILAALFNAVFL
                     LVAVGAIGWEAILRLFRPEAVAGETVIVVAAIGILINGITAWLFVSGSKSDLNIRGAY
                     LHMVSDAAVSAGVVLAGLAILYTGWLWLDPVVSLVIVGVIVWGTWSLLRESLAMSLHA
                     VPPGLDSAAVHALLEKQPGVQAVHDLHIWPMSTTDIALTAHLLIPSGHPGDAFLMHLS
                     EELRAHHGIGHVTLQVETDPATTCVLESDNVV"
     gene            complement(1944..>2467)
                     /locus_tag="PKV23_06750"
     CDS             complement(1944..>2467)
                     /locus_tag="PKV23_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HQX85170.1"
                     /translation="EIDDKYRFLKPGLVVVDLGAAPGGWCQVASKRVKAEEGRGKIIA
                     IDMHGIDPLPAVTVFKKNFLDDDAPQLLFDALGGRKADVVLSDMAAHATGHRQTDHIL
                     VMTLAEAGLDFARQVLAPGGTYLAKVLRGGTEGEMLKQMKRDFSSVRHVKPMASRDDS
                     AELFVLAMGFRGA"
BASE COUNT          442 a          829 c          668 g          528 t
ORIGIN      
        1 ggagtcagcc ccggcgccga tcaacgcctt caccgcttca cccgtcgcca cgtttcccgc
       61 gacgatctgc accttgttgg aaagcttctt gatgcggttg acctggttga tcacatgaat
      121 ggaatggcca tgcgccgtat ccaccacgat gatgtcaaca cccgcatcga tgagcatctc
      181 ggctcgcaga aatccctttt cacccgtgcc cgtggccgcc gccacacgca accgctcctg
      241 ctcgtccttg gccgcaaacg gatggtccgc cgccttttcc atgtcgttga cggtaatcag
      301 cccaacgcat tcataatcct cattcaccac gatgagtttc tcgatccggt gcttgtgcag
      361 aaggcgcttg gcttcaagcc ggtcgacgcc cgccttcacc gtgatcaggt tgcgcgtcat
      421 caggtcggaa actttggtgt tcatgtccgt ggcaaagcgc acatcgcggt tggtgatgat
      481 gcccgccagc ttgccattct gctccaccac cgggatgccc gaaatcctgt accggtcctt
      541 gagctgcatg acctcggcta aggtggcttc aggccggatg gtaatcggat tgaccaccat
      601 gcccgattca aagcgcttca cctgccgcac gtgctcggcc tgcacatcgg gttccaggtt
      661 tttgtggatg acgccgagcc cgcctgcctg ggccatggca atggcgagtt tcgattcagt
      721 caccgtatcc atggccgcgg aaatgatagg aatggaaagc tggatgtcgc gggttatgtg
      781 cgtggccgtc gaaacctcgg ccggcaacac ggacgaagca ccgggcttca tcagcacgtc
      841 gtcaaaagtc aaatattccg gaatttggcg gagagccatg ggggccaacc tttcaggtat
      901 ttcaggattc gttgagttgg cagcggcttc taacacctga atgacggcgg gagcaagcgg
      961 tgaatcggca attgccaaac cctggttaag gcggcaaaag cccctgacgg gagtaccacg
     1021 atgggccacg cccatgacca cgatcttcat gcccctggcc acgttcatgc gcccgcctcc
     1081 ttcggcaagg cctttgcaat cggtattgcg ctcaatctgg cctttgtact gatcgaggct
     1141 ggctacggca tcgttgccaa ttccatggcc ctgctggccg atgcgggcca caatctctcg
     1201 gatgtcctgg gcctcgtcgt cgcctggctt gccgcggttc tctccaaccg cccgccctcc
     1261 cggcgctaca ccttcggcct caaaagctcc acgatccttg ccgcgctttt caatgccgtg
     1321 ttcctgctgg tggccgtggg tgccatcggc tgggaggcaa tcctgcgcct tttccgcccg
     1381 gaagccgtgg ccggcgaaac cgtaatcgtc gtcgccgcga tcggcatcct gatcaacggc
     1441 ataaccgcct ggctctttgt ctcaggcagc aagagcgatc tgaacatccg cggcgcctac
     1501 ctccacatgg tcagcgatgc cgctgtctcg gcaggggttg tccttgccgg tctcgccatc
     1561 ctttacaccg gttggctttg gcttgatccc gtcgtcagcc tggtgatcgt gggcgtcatt
     1621 gtctggggca cctggagcct gctgcgcgaa agcctcgcca tgtcacttca cgccgtgccg
     1681 ccgggtcttg attccgccgc cgtacacgcc cttctcgaaa aacagcctgg cgtccaggcg
     1741 gtccatgatc ttcatatctg gcccatgagc accaccgaca tcgcgctcac cgcccatctt
     1801 cttatcccca gcggccaccc cggcgatgca ttcctgatgc acctctccga ggagctccgc
     1861 gctcatcacg gcatcggcca tgtcaccctg caggttgaaa cggatccggc cacgacgtgt
     1921 gttcttgagt ccgacaacgt cgtctaggcg ccgcgaaatc ccattgccag aacgaacagc
     1981 tccgccgagt catcccggct cgccatgggt ttcacatggc gcacgctgga aaaatccctc
     2041 ttcatctgct tgagcatttc gccttccgtg ccgccgcgca ggaccttggc cagataagtg
     2101 ccgcccggcg ccagcacctg gcgggcaaaa tcaagccccg cttcagccag ggtcatcacc
     2161 aggatatggt ccgtctgccg gtgccccgtg gcgtgcgccg ccatgtcgga cagcacgaca
     2221 tcggctttcc gcccgcccag cgcatcgaac agaagctgcg gagcgtcatc atcgaggaag
     2281 tttttcttga aaaccgtcac tgccggaagt ggatcaatcc cgtgcatgtc gatggcgatg
     2341 atcttgcccc gcccctcttc cgccttcacc cgcttggagg ccacctgaca ccagccgccc
     2401 ggtgcagctc ccagatcgac caccacaagc cctggcttca aaaacctgta cttgtcgtca
     2461 atctcga
//
LOCUS       DAORVN010000301         2466 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_41837_length_2466_cov_3.162173, whole genome shotgun sequence.
ACCESSION   DAORVN010000301 DAORVN010000000
VERSION     DAORVN010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2466)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41837_length_2466_cov_3.162173"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(10..1434)
                     /locus_tag="PKV23_06755"
     CDS             complement(10..1434)
                     /locus_tag="PKV23_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015315772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQX85171.1"
                     /translation="MTDESAARTNWGELWRSGALPRFCFISLGLTFHSGAENMISTIM
                     PAMVREIGGVELNGWVFAIYEIGSIIAGAVTGRLTTYWSVRTNMIVAALIFTLGTLET
                     ALSATMYWALIGRLISGFGGGALISLSFVAVQRYFPSSIWPQLMAVFSVVWGVSAFGG
                     PLYGGIMQTLLSWRWAFLIFAAAAVVFAIACVPLLRNEPERPSASLEPRNFPALALTC
                     LALGITAIATAGVESRFYVSQALVIAGLAGIAAFFVLDARNKTSRLFPTAPFNPRTVV
                     GSGMLMVAALSVSTCSFGFYGPLLLASLHDFSPVTTGLIIASESISWSILSILVATAP
                     KQHEVLIVRAGALMIAAGVAGFAWVVPSGSMAGLLFFATLQGGGFGILWPFANRRVVE
                     AALPHERELAASAFSTLQRMGYAVGSATAGIIANANGFSGGFTKAAATTAAPPLFLYF
                     IPIALLGCVAAFRLTGNRADGPGR"
     gene            complement(1661..1867)
                     /locus_tag="PKV23_06760"
     CDS             complement(1661..1867)
                     /locus_tag="PKV23_06760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85172.1"
                     /translation="MKKLAISLIALAAVSTASFASIRDVTTPYGAQDTQNAASVSTSN
                     AFAVPGATGGMAGDDLGLGGSNRR"
     gene            2238..>2466
                     /locus_tag="PKV23_06765"
     CDS             2238..>2466
                     /locus_tag="PKV23_06765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85173.1"
                     /translation="MANTPPPVWVYNETIKMFATPGDPPFEDRHELETHWGRAWGINN
                     DVGRMRSVLVHRPGKEMDVIDPKKRIESIGSF"
BASE COUNT          579 a          686 c          788 g          413 t
ORIGIN      
        1 tgcagatgat tagcggccag gcccatccgc cctgtttccc gtcaatcgga acgcggcgac
       61 gcagccgagc agtgcgatgg gaatgaaata gagaaacaag gggggtgctg cggtggttgc
      121 ggcggccttg gtgaagccgc cggagaagcc gttggcattg gcgatgatgc ccgccgtggc
      181 gctgcccacg gcgtagccca tccgctgcag cgttgagaag gccgaggcgg caagctcgcg
      241 ctcatgcggc agggcggctt caacaacgcg gcggttggca aaaggccaga ggatgccaaa
      301 accgccgccc tgaagcgtgg cgaagaagag cagcccggcc atggacccag atggcaccac
      361 ccaggcaaat ccggcaacgc cggcggcgat catcagggct ccggcgcgga cgatcaacac
      421 ctcatgctgc ttgggtgcgg tggccacaag aatggacaaa atggaccagg agatcgactc
      481 gctggcaatg atcaggccgg ttgtcaccgg agagaaatca tggagagagg ccagcagcag
      541 aggtccgtaa aaaccaaacg aacaggtgga tacggagagt gccgccacca tcaacatgcc
      601 ggagcccacg actgtccggg gattgaaagg cgccgttgga aacaggcggg aagtcttgtt
      661 gcgggcgtcg agaacaaaga atgctgcgat gccggcaagc cctgcgataa caagcgcctg
      721 ggaaacatag aagcgcgact ccaccccggc ggtggcaatc gccgtaatac ccagggcgag
      781 gcaggtgaga gcaagggcag gaaaattgcg tggctccaag cttgcgctgg ggcgttccgg
      841 ttcattgcgc agcagaggaa cacaggcgat tgcgaagacg acagctgcgg cagcgaaaat
      901 cagaaatgcc cagcgccagg acagcagcgt ctgcatgatg ccgccataga gcggaccgcc
      961 gaaggcgctg acgccccaga cgaccgagaa aaccgccatg agttgcggcc aaatggacga
     1021 cgggaaatag cgctgcactg cgacaaagga gagcgagatg agagcgccac cgccgaaccc
     1081 gctgatcagg cggccgatca gggcccagta catggtggcc gacagggcgg tttcgagggt
     1141 gcccagggtg aagatgagag ccgccacaat catgttggtg cgcaccgacc agtaggtggt
     1201 gagccgcccg gtgacggcgc cggcaatgat cgagccgatc tcatagatgg caaaaaccca
     1261 gccgttcagt tccaccccgc ctatttcgcg caccatggcc ggcatgatgg tcgagatcat
     1321 gttttcggca ccggaatgga aagtcaggcc aaggctgatg aaacagaagc gcggcagcgc
     1381 gcccgagcgc cacaactctc cccagtttgt gcgcgcggcg ctttcatcgg tcatggctgg
     1441 atagggttcc tgtgggcgca agggcgcaca ccggcaatgc tgtcctcgcc tgcctgagtc
     1501 acgccctttg gtggaaaaca tagaagctga atggattcta tatgggcgtt caataaagcg
     1561 caagaaccgc cagtcaggga tccggccacg tgcaaaatgg ttgcggccgg actgttggtc
     1621 cggccactgt ctatgcaaac gtatggcgta accgcaaata ctagcggcgg ttgctgccgc
     1681 cgaggcccaa gtcgtcgccg gccatgccgc cggttgcgcc aggaactgca aaggcattcg
     1741 aggtcgaaac gctggctgca ttctgcgtgt cctgtgcgcc gtatggcgtg gtcacgtcac
     1801 ggatcgaggc aaacgaagcc gttgaaacag cggcgagggc gatgagggaa attgcgagct
     1861 tcttcatggt agtctcctgt tgagtaggtg gttggttcaa tctgtttccg ggttgcgctg
     1921 accgcttccc gatgacctca atctatgcct ttgcgcggca aaatcgaatc aagcccagtt
     1981 acagattggc gatcaactta tctaaccatt tcatcaggtt accggttcgc atggaaggag
     2041 aattcaactc ctgctcacga cgaaaaaccg gaccacacaa cgcggtgatt gaaagggggc
     2101 tttttttgat caagaaaagc ccaggccaga aaataatcgg attgacgatc ccgctgtgct
     2161 tttcctcatg cgacggagag tgatggcgcg gactgcattc atcatgttac acacggtctt
     2221 tcgcgaggga gcacatgatg gcaaatacgc cgccgccggt ttgggtttac aacgagacga
     2281 tcaagatgtt cgccaccccc ggcgatccgc cgttcgagga caggcacgaa cttgaaaccc
     2341 attggggccg cgcctggggc atcaacaacg acgtgggccg catgcgctcg gtgctggtgc
     2401 accggcccgg caaggaaatg gatgtcattg atcccaagaa gcgcattgaa agcatcggct
     2461 ccttcg
//
LOCUS       DAORVN010000302         2465 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_41879_length_2465_cov_2.595851, whole genome shotgun sequence.
ACCESSION   DAORVN010000302 DAORVN010000000
VERSION     DAORVN010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2465)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2465)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2465
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41879_length_2465_cov_2.595851"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..751)
                     /locus_tag="PKV23_06770"
     CDS             complement(<1..751)
                     /locus_tag="PKV23_06770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85174.1"
                     /translation="MIRKFAVRAVIGLATLAMPIASIGSVQAIGPGGSYSSGIACVNL
                     GSASASIAITFYAADSGTAITTYSVPSAVGPKANVVLFTPDIPGLPGALQGSAVVSSD
                     QQVACTADAQRSDGTPGTNATPARASTSESFDTTQAGSTLYVPQVAKQLGQASTGFFD
                     SYIAVQNIESTAVTATVSYVDRFGVAYPAASESASIPPQASHYFYQGANANLPGNLNG
                     TAKIVASDPSKKLAATAVVYNDGTTISKSQMS"
     gene            complement(863..1699)
                     /locus_tag="PKV23_06775"
     CDS             complement(863..1699)
                     /locus_tag="PKV23_06775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85175.1"
                     /translation="MPQAFSLLGISGLAGTVVLAGLINLLGGLAWSLIASAVFPGISI
                     ISATRMFFLTLPMKYVPGSVWSHVGRAGWLERTRSLPSPPEGLIKRSGLLVVAADFAL
                     LLSSGLVATLISGLSSMPAVFQGRVGPQFWNACLVLAICVFGATPFVFGSLIGRRGRG
                     PLLVFARRIWTAQVVQIIDWLIGGFLLGVLVTALQAPLNRVVVLIGDSTLALYAGIMA
                     GLAAIIVPNGLGIREMAMSVMYGDVATVEAGFIAGFLYRALSMLTELLLFGLSALRGG
                     RD"
     gene            complement(1818..>2465)
                     /locus_tag="PKV23_06780"
     CDS             complement(1818..>2465)
                     /locus_tag="PKV23_06780"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HQX85176.1"
                     /translation="VVHLGVDAGFRPRSTQEIAPTLARRQIGPRYVLHVGAVSAKKNL
                     VSLVRAFAILKGNGYPGQLVLVGPDYQKLERVPLAQVAEQAGITDSVRIVGAVSDDDL
                     KDIMAGAELFAFPSKYEGFGIAVVEAMACGVPIIAARAGAVVEVVGDAAPIIDDGADF
                     ADLAFQMGLLLADEVRRQKVASACLRRASLFTWERVAHGTMAVYEKRLGASSAAH"
BASE COUNT          580 a          754 c          699 g          432 t
ORIGIN      
        1 cagacatctg tgatttcgaa atcgtggtgc catcgttgta gacaacggcg gtcgcggcga
       61 gcttcttcga aggatcgctg gccacaatct tggcggtgcc gttgaggttg ccgggcaaat
      121 tggcattggc gccctggtaa aagtagtgcg aggcttgcgg cggaatggaa gccgactcac
      181 ttgccgccgg gtacgccacg ccaaaacgat cgacatacga aaccgttgcg gtcacggctg
      241 tgctctcgat gttctgaaca gcgatatagc tgtcaaagaa accggtggag gcttggccaa
      301 gctgcttcgc gacctgtggc acgtagagcg tgctacccgc ctgggtggtg tcgaagcttt
      361 cgcttgtgct cgcacgggct ggagtggcat tcgtccctgg cgtgccatcg gagcgctgag
      421 catccgccgt gcaggctact tgctgatccg aactgacgac cgctgaaccc tgcagggccc
      481 ccggcagacc gggtatgtcc ggagtgaaga gcactacatt ggctttcggc ccaaccgcag
      541 acggaaccga ataggtggta atcgccgtgc cgctatcggc cgcgtagaac gtgatggcga
      601 tcgacgctga cgccgatccc aggttcacgc aagcaattcc gctcgagtaa ctgccgcccg
      661 ggcctatagc ttgaaccgag cctatcgagg caattggcat ggcgagcgtg gccaaaccaa
      721 tcaccgccct aactgcaaat tttctaatca tctgaatctc catttttatg tcaagtagca
      781 gactgaactg tgaacaacta ctactatcca ggaactcgcg gcggtttcaa tagcccgttc
      841 gggttaatga cccagataac actcagtctc gaccgcctcg aagcgccgaa agaccgaaca
      901 atagtagttc cgtaagcatc gaaagtgcgc gatacagaaa gcctgcgatg aagcctgcct
      961 caacggtggc aacatcgcca tacataactg acatcgccat ctcccgaata cccaaaccat
     1021 ttggcacgat gatggctgcc agaccggcca tgattcccgc atagagcgca agggtggaat
     1081 ccccaatcag cacaacaacg cggtttagcg gagcctggag ggcggtaaca agtacaccca
     1141 gcaggaagcc tccaatcagc cagtcgataa tctggacgac ttgggcggtc cagattctgc
     1201 gtgcgaagac gagcagcggc ccccggcccc gtcggccgat caaactcccg aaaacgaacg
     1261 gggttgcgcc gaagacacaa atggcgagta ccaggcaggc gttccagaac tggggcccga
     1321 cacgtccctg aaacacggca ggcatcgacg agaggccgct gatcagcgtg gcgaccaatc
     1381 cggaggacag caaaagggcg aaatcagccg caaccaccag cagtcctgag cgctttatca
     1441 agccctccgg tggtgaagga agtgaacgcg tccgctcgag ccagcccgcc cgcccgacgt
     1501 gactccagac cgatcccggg acatatttca tgggcagggt caagaagaac atgcgcgtgg
     1561 cggaaatgat cgaaatgccg gggaatacag cgctggcaat tagcgaccac gccagaccac
     1621 ccagcaaatt gatcaaaccg gccaggacga cggtcccggc cagccccgaa attcccagga
     1681 gtgagaacgc ctgcggcata tcaatccgct gagcggttgt ccatagaccc catagcatta
     1741 gggccgtgcc cgcggcaagt cctgccagtt tggcgggccg cccgcgcacc acacgccgaa
     1801 cggcgctcgc caccgcccta atgcgcggct gaactcgcgc caagtcgctt ttcatagacg
     1861 gccattgtgc cgtgggccac acgttcccag gtgaacaggc tggctcgacg aagacacgcg
     1921 ctggcaacct tttggcgcct tacttcgtcg gcaagcaaaa gacccatttg gaaggccaaa
     1981 tccgcaaagt ccgcgccatc atcgataatt ggcgccgcat cacccactac ttcaactacc
     2041 gcgcctgccc gggcggcgat aatcggcacc ccacacgcca tcgcctcgac gacagcgatc
     2101 ccaaatccct cgtattttga cggaaacgcg aacagctcgg cgccagccat gatgtcttta
     2161 agatcatcat ccgaaacagc gccaacaatc cgcacagaat cggttatgcc cgcctgttcg
     2221 gcaacttgag caaggggaac gcgctcaagc ttctgatagt ccgggccaac aaggaccagc
     2281 tggcccggat agccattgcc cttcaggatc gcaaatgcac ggacaagcga gaccaggttc
     2341 tttttggcgg aaactgcgcc tacgtgcaag acatatcttg ggcctatctg tcggcgagcg
     2401 agggtggggg caatctcttg tgtggatcgc ggcctgaatc cggcgtcaac gccgaggtgg
     2461 acgac
//
LOCUS       DAORVN010000303         2456 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_42141_length_2456_cov_3.099958, whole genome shotgun sequence.
ACCESSION   DAORVN010000303 DAORVN010000000
VERSION     DAORVN010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2456
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42141_length_2456_cov_3.099958"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1456
                     /locus_tag="PKV23_06785"
     CDS             <1..1456
                     /locus_tag="PKV23_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019966163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HQX85177.1"
                     /translation="LAAMANPADVLAGINVGKYVKKEYRDQYKLGDNDELWDPNKDWI
                     RTVDWEAVRKEHAGKTVRFAIGAADRESAQDGIKPFLELSGINVELVAIPDDSMYDKV
                     VAEFLSGNASFDALQFFSPWLGDFAAQGFLKPLDEYAAKWKLPVDDFYETYLMNYGHW
                     GDKGLMGIPFDCDIQMVHIRPSIFKKVLGGDVDRVKSIPSYDEMIRLAPELNKAEPGV
                     NAIGMMCGRGFWATYTWQHVAAQYGLELFDDKWEPIFNGDAGVKGLETIVALSKHAIE
                     GVAAADWPTNRAAWLGGQVACNISWQDSGTQATRPDQSKVGDDVLTIYEPRVEGGRFA
                     PPNIAGSTSCVTSTSPEPEAAFLMLAFLTSASIMAMNEANANGVAPGYKSVLTNEKLR
                     AVSQPAKIWSEELEHAWCAPRLPNAFQMDQEIGFLINKVVVGEMQPKAALDEAAAKVK
                     AIMEKSGFYKGADPVSYASMAPGTWVGKDKKLPF"
     gene            1599..2450
                     /locus_tag="PKV23_06790"
     CDS             1599..2450
                     /locus_tag="PKV23_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019966164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HQX85178.1"
                     /translation="MRKAFPYLLLGPAVVYLLGITLYPGIYAVYRSLMTGKFKLEYAG
                     LQNYRELLVDNAFWTSLSNTLILGVITLALEFVIALSLAALVYRDRWVKAWRILFLLP
                     MLFMPSAVSFLWKLLFNDGRVISDLLTRVGFGAGNIDFMGSTALARLVLVVTDVWQWT
                     PFLFIIFVAALQGMDKEVEEAARLDGASWRKIFFNISLPMMRPVIAIALVLRGIDILT
                     MFASVHIITQGTPGGNTKTLSYFIYRTAFKSFEQGYASAASVLLLIATIILAQTLVRR
                     FFKSGVE"
BASE COUNT          487 a          717 c          715 g          537 t
ORIGIN      
        1 cctagccgcg atggctaacc cggccgatgt tttggctggc atcaatgtcg gaaaatatgt
       61 gaagaaggaa tatcgcgacc agtacaagct gggcgacaat gatgaattgt gggatccgaa
      121 caaggactgg atccgcaccg tggactggga agcggtgcgc aaggaacatg cgggcaagac
      181 ggttcgcttc gccattggcg ccgcagaccg tgaatcggcc caggatggca tcaagccgtt
      241 ccttgaactc tccggcatca atgttgaact ggtggccatt cccgatgact cgatgtacga
      301 caaggtcgtg gccgagttcc tctcgggcaa tgccagcttc gatgcgctgc agttcttctc
      361 gccatggctt ggcgatttcg cggcccaggg cttcctcaag ccgctggatg aatatgccgc
      421 caagtggaaa ttgccggtcg atgatttcta cgaaacctat ctgatgaatt acggccattg
      481 gggcgacaag ggcctgatgg gcattccgtt cgattgcgat atccagatgg ttcacatccg
      541 gccgtcgatc ttcaagaaag tccttggcgg ggatgtggac cgggtaaagt ccattccatc
      601 ttatgatgaa atgatccgcc ttgcgccgga actgaacaag gcagaacccg gggtgaatgc
      661 catcggcatg atgtgcggcc gcggcttctg ggcaacatat acctggcagc atgtcgcggc
      721 ccagtatggc ctcgagctgt tcgacgacaa gtgggaaccc attttcaacg gcgatgccgg
      781 cgtcaagggc ctcgagacca tcgtggcact atcgaagcac gctattgaag gcgtggccgc
      841 ggccgactgg ccgaccaacc gcgccgcctg gctcggcggc caggtcgcct gcaacatctc
      901 gtggcaggat tcgggcaccc aagccacgcg tcccgaccag agcaaggtcg gcgatgacgt
      961 cctgacgatc tatgaaccac gcgttgaagg cggccgcttt gccccgccca acatcgcagg
     1021 ctcaacgtct tgcgtcacct ccacctcgcc ggagccggaa gccgcatttc tcatgctggc
     1081 cttcctgacc tcagcttcga tcatggcgat gaacgaagcc aacgccaatg gcgttgctcc
     1141 gggctacaag tcagtgctga ccaatgaaaa gctgcgtgcg gtttcacagc cggccaaaat
     1201 ctggtctgaa gagctggagc acgcctggtg cgcgccgcgc ttgccaaacg ccttccagat
     1261 ggaccaggaa atcggcttcc tgatcaacaa ggtggttgtg ggcgaaatgc agccgaaagc
     1321 cgcccttgat gaagcggcgg ccaaggtcaa ggcgatcatg gagaagagcg gcttctacaa
     1381 aggcgccgac ccggtatcct atgcttcgat ggcccctggc acgtgggttg gcaaggacaa
     1441 gaagctgccg ttctaaactc tgcagcgcga aaatggccgg ggcgacccgg ccattttttc
     1501 attccttaac gatgcgaagc atgtaaacac tttccatggc tgacgtggcg ctccccctac
     1561 atccagcgaa gcggaccctg gtccgccgca gccctttcat gcgcaaggca tttccttatc
     1621 tgctattagg cccggccgtc gtttatctgc tgggcatcac gctttatccc ggcatttacg
     1681 cggtttaccg cagcctgatg accggcaagt tcaaacttga atatgccggt ttgcagaatt
     1741 accgcgagct tctggtcgac aatgccttct ggaccagcct gagcaataca ctcattcttg
     1801 gcgtgatcac cctggcgctt gaattcgtca tcgccctgtc gctggcggcg ctggtttacc
     1861 gtgaccgctg ggtgaaggcc tggcgcatcc tgttcctgct gccgatgctg ttcatgccgt
     1921 ctgccgtttc atttctgtgg aaactcctgt tcaacgatgg ccgggtgatc agcgacctgc
     1981 tgacccgcgt tggattcggc gctggcaata tcgacttcat gggttccacc gccctcgctc
     2041 gccttgttct ggtggtgacc gacgtatggc aatggacgcc cttcctgttc atcatttttg
     2101 tggccgcgct gcagggcatg gacaaggaag tggaggaagc cgcccgtctt gacggggctt
     2161 cctggcgcaa gattttcttc aatatttcgc tgccgatgat gcggccggtg attgccatag
     2221 cgctggtgct gcgcggcatc gacattctca ccatgttcgc ctcggtgcat atcatcaccc
     2281 agggcacgcc gggcggaaac accaaaaccc tgagctactt catttaccga acggccttca
     2341 agagttttga acagggctat gcttcggcgg cctctgtcct gctgctgatc gccacgatca
     2401 ttctggcgca gacgctggtg cggcgcttct tcaagtcggg agttgagtga tggcgg
//
LOCUS       DAORVN010000304         2448 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_42365_length_2448_cov_2.990807, whole genome shotgun sequence.
ACCESSION   DAORVN010000304 DAORVN010000000
VERSION     DAORVN010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2448)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2448)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2448
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42365_length_2448_cov_2.990807"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(46..522)
                     /locus_tag="PKV23_06795"
     CDS             complement(46..522)
                     /locus_tag="PKV23_06795"
                     /inference="COORDINATES: protein motif:HMM:NF024865.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HQX85179.1"
                     /translation="MDSTLACTQSPETLRHLRALNARFIHNYVTNDVPSHDAILHPDF
                     ICLASNGMRIDRASYLRRWANLFDPEVVIYWDTRDELITVHGTVALVRATNKHTERHN
                     GVETTGMTNYTDTYVYEHGAWKCIQAHLTPVAPEHWPPDSTIVSVYIKGKCQARSL"
     gene            complement(541..1365)
                     /locus_tag="PKV23_06800"
     CDS             complement(541..1365)
                     /locus_tag="PKV23_06800"
                     /inference="COORDINATES: protein motif:HMM:NF037627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HQX85180.1"
                     /translation="MDAPMTKQKQGADGKFSHLLRHWRQRRRLSQLELAGEANVSARH
                     LSFLESGRSQPSRDMVQLLGNALDLPLGERNVLHLAAGFVPPYGTPEQAAEDIPHVRQ
                     ALDFMLRQQEPFPAIVIDGHWDIRLRNDATKRMFSVFRQSYEVEPAIADNAMHTVFHP
                     KALRQFIVNWEEFAGGMIQILHREVAQGSRMSAQLLDEIMAYPGLSAFAKPARAPETS
                     SPVMTMQLQKGYLRLDFFSTFTTFAMPADAALQQLKIECFYPADDATAEIARKLAT"
     gene            complement(1430..2359)
                     /locus_tag="PKV23_06805"
     CDS             complement(1430..2359)
                     /locus_tag="PKV23_06805"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQX85181.1"
                     /translation="MSYLGKNGYSSCPSAPGLDHKTVALPIELPAGVSAPLGPPASPC
                     APRRPWPLAKGGDARAMAMMGALYQLGNGVKPDLEKAVSWYTRAAEKNHPGAQFSLAM
                     LYLDGSLGNPDADKGALWLEKAATGGNPQAQYNLGLLYTGVYGTPPEWPKAAEWFRKS
                     ADQGFAEAQYNLGLLYLDGNGVERSHVNAATWFSRAAVQGMPEAAMDYGVLVFRGEGV
                     QRNEAVGAKWMLVAARRGNIVAQNRIAKLFATGHGVALDPIEAMKWNFLASKGGRTDP
                     WLDDFAAKQPEAQRKDAETRAAAFKPEAANSGS"
     gene            2345..2417
                     /locus_tag="PKV23_06810"
     tRNA            2345..2417
                     /locus_tag="PKV23_06810"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2378..2380,aa:Thr,seq:tgt)
BASE COUNT          467 a          672 c          804 g          505 t
ORIGIN      
        1 tagcaggcct tggcggggaa ggctagagcg gccaccgggg atgctttaca acgaccgcgc
       61 ctggcatttt cccttgatgt agacgctgac gatcgtgctg tcagggggcc agtgctcggg
      121 agcaaccggg gtgaggtggg cctgaatgca tttccaggct ccatgctcat acacataggt
      181 gtcagtgtag ttcgtcatgc cggtggtttc cacgccattg tggcgctccg tgtgcttgtt
      241 ggtggcgcgc accagcgcca ccgtgccatg gaccgtgatg agttcgtcgc gggtgtccca
      301 gtaaatgaca acttcgggat cgaacaggtt cgcccagcgc ctgaggtaag aggcgcggtc
      361 aatccgcatg ccgttgctgg ccaggcagat gaagtcggga tggaggatgg catcatgcga
      421 gggcacgtcg ttggttacgt agttatgaat gaagcgggca ttcagggcgc gcaggtggcg
      481 caaggtttcg ggtgactggg tgcaggccag agtggaatcc atcgttgtgt cctgttttgg
      541 ttaggtggcg agtttacgtg cgatctcggc ggtggcgtca tcggccggat aaaagcactc
      601 gattttcaac tgctgcaaag cggcatcggc cggcatcgca aaggtcgtga aagtggaaaa
      661 gaagtcgagc cggaggtagc ctttctgaag ctgcatggtc atgaccggcg atgacgtctc
      721 aggcgccctt gcgggctttg cgaaagcgga caggccggga taggccatga tctcgtccag
      781 gagctgggcc gacatccggc tcccctgggc tacctcgcgg tgaagaatct ggatcatccc
      841 gccggcgaac tcttcccagt tgacgatgaa ctggcgcaaa gccttgggat ggaacacggt
      901 gtgcatcgcg ttgtcggcga tggcgggctc cacttcgtag gactggcgga aaacagaaaa
      961 catgcgcttg gtcgcatcat tgcgcagccg gatatcccag tggccatcga tgacaatggc
     1021 gggaaagggt tcctgctggc gaagcatgaa gtcgagcgcc tggcgcacat gagggatatc
     1081 ttcagcggcc tgttccgggg tgccataggg aggaacaaag ccggcagcaa ggtgcaggac
     1141 attgcgttcc ccaagcggaa gatcgagcgc gtttccaagc agttgcacca tatcgcggct
     1201 gggctgcgaa cggccggatt caaggaagct caaatgcctg gccgaaacat ttgcctcgcc
     1261 tgccagttcg agctggctca gcctgcgccg ctggcgccaa tgcctgagca gatggctgaa
     1321 cttgccatcg gcgccttgct tttgctttgt catcggtgcg tccatggagg ctacgtagag
     1381 cctgccatgc tagcgcgcaa ttacctccga ggtaagcaag cggttgagtc tagcttccgc
     1441 tattggctgc ttccggcttg aaggcggcgg cgcgggtttc ggcgtctttg cgctgggctt
     1501 cgggctgctt ggcggcaaaa tcatcaagcc aggggtcggt gcggccgcct ttgcttgcaa
     1561 ggaaattcca tttcatcgct tcgatgggat caagggccac accgtggccg gttgcaaaaa
     1621 gttttgcgat gcggttttgc gccacgatgt ttccgcgccg ggcggcaacc agcatccact
     1681 ttgcaccgac ggcttcgttg cgctgcacgc cttcgccacg gaacaccagc acgccgtaat
     1741 ccatggcggc ttccggcatg ccctgcacgg cggccctgga aaaccaggtg gcggcgttga
     1801 catggctgcg ctcgacgcca ttgccgtcca gatagaggag gcccaaatta tattgcgctt
     1861 cggcgaaacc ctgatcggcg gatttcctga accactcggc ggctttcggc cattcaggcg
     1921 gcgtgccgta gacacccgtg tagagcaggc cgagattata ctgggcttgc ggattgccgc
     1981 cggtggcggc tttctccagc cacagggcgc ccttgtcggc atcgggattg ccgagcgaac
     2041 cgtcaaggta aagcatggcg agcgagaact gcgcgccggg atgatttttc tcagcggccc
     2101 tggtgtacca ggaaacagcc ttctcaagat caggcttcac gccattcccc aactggtaga
     2161 gcgcacccat catggccatg gcgcgggcat cgccgccctt ggccagaggc caaggacggc
     2221 gcggcgcgca aggactggcg ggcggcccca gcggggccga aacccccgcc ggtagctcaa
     2281 tgggtagagc aacggttttg tgatcgaggc caggggccga tgggcaggag ctgtatccat
     2341 ttttgccgag gtagctcaat tggtagagca acggttttgt aaaccgtagg ttgtgggttc
     2401 gactcccgtc ctcggctgat tttgaaacac gaaactatgc gtaaattt
//
LOCUS       DAORVN010000305         2442 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_42551_length_2442_cov_3.817763, whole genome shotgun sequence.
ACCESSION   DAORVN010000305 DAORVN010000000
VERSION     DAORVN010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2442
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42551_length_2442_cov_3.817763"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..517
                     /locus_tag="PKV23_06815"
     CDS             <1..517
                     /locus_tag="PKV23_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004430264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HQX85182.1"
                     /translation="LQLLTAKPVLFVCNVEEASAASGNSQSARVAEMAAKQGAASVVI
                     SARIEEEISQLAEDEAAMFLEEMGLHEAGLDRLIKAGYALLGLQTYFTVGPKEARAWT
                     ITKGTLAPQAAGVIHGDFEKGFIRSETVAYEDYIAGNGEAGAKEAGKFRVEGKTYEVK
                     DGDVLHFLFSG"
     gene            727..1272
                     /locus_tag="PKV23_06820"
     CDS             727..1272
                     /locus_tag="PKV23_06820"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HQX85183.1"
                     /translation="MNHLYYGDNLTVLRDSIRDESVDLIYLDPPFNSNASYNVLFKGP
                     QGTESAAQIEAFDDTWHWNDSAEAAFGDVMRGGNAAASTMLRAIRSFLGDNDMMAYLA
                     MMAVRLLELHRVLKPTGSLYLHCDPTASHYLKILLDAVFGAGNFRSELVWKRTTAHSA
                     SKRWADVHDTILFYTRTSDFI"
     gene            1600..2369
                     /locus_tag="PKV23_06825"
     CDS             join(1600..1936,1936..2369)
                     /locus_tag="PKV23_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611979.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HQX85184.1"
                     /translation="MSNLFWLTDEQVERLRPFFPKSHGKPRVDDRRVLSGIIFINRNG
                     LRWCDAPKEYGPSKTLYNRWKRWGDMGVFARMMEGLASEGTEQKTIMIDATYLKAHRT
                     ASSLRAKKGGPEDQRGRLIGRTKGGLNTKLHAVTDAKGRPLKFFMTAGQVSDYTGAAA
                     LLGSLPAAEWMLADRGYDADWFRDALKDKGIRPCIPGRKSRGKTVRYDKRRYRRRNRI
                     EIMFGRLKDWRRVATRYDRCAKTFLSAVALAATVIFWL"
BASE COUNT          513 a          647 c          819 g          463 t
ORIGIN      
        1 gctgcagctt ctgaccgcaa agccggtgct tttcgtctgc aacgtcgaag aggcgtcggc
       61 ggcgtccggc aacagccagt cggcgcgggt ggccgagatg gcggcgaaac agggcgcggc
      121 ctcggtggtg atctcggccc ggatcgaaga ggaaatcagc cagcttgccg aggatgaagc
      181 cgcgatgttc ctggaagaga tggggctgca cgaggcgggg ctggatcggc tgatcaaggc
      241 gggctacgcg ctgctgggct tgcagaccta tttcaccgtg gggccgaaag aggcgcgggc
      301 ctggacgatc accaagggga cgctggcccc gcaggcggca ggggtgatcc acggcgattt
      361 cgagaagggc ttcatccggt cggaaaccgt ggcctatgag gattatatcg ccgggaacgg
      421 cgaggcgggg gcgaaggaag cgggcaagtt ccgggtcgag ggcaagacct acgaggtcaa
      481 ggacggggac gtgctgcatt tcctgttcag cgggtaagtg tccacggtgg acatttctgg
      541 attatgcttt gggtccagag ggttgtctgc gggcgttagg gatttggtaa gggttgcgga
      601 gtcggggtga accccgacct acggtgttgc ccgattgcct tggcttggct aaccttgtct
      661 taaccgccgg tcggcaatgg tggggcaaga tgtcgggctg aagcccgacc tacggggcgg
      721 gcaagcatga accatctgta ttacggcgac aacctgaccg tgctgcgcga cagcatccgg
      781 gatgaaagcg tcgacctgat ctaccttgac ccgccgttca attccaacgc cagctacaac
      841 gtgctgttca aggggccgca aggcaccgag tccgccgcgc agatcgaggc gtttgacgac
      901 acctggcatt ggaatgacag cgcggaagcc gcgttcggcg acgtgatgcg cggcggcaat
      961 gcggcggctt cgacgatgct gcgggcgatc cggtcatttc tgggcgacaa tgacatgatg
     1021 gcttacctcg ccatgatggc ggtgcggttg ctggagttgc accgggtgct gaagccgacg
     1081 gggagcctct atctgcactg cgacccgacg gcgagccatt atctgaagat cttgctggat
     1141 gcggtgtttg gggcggggaa ttttcgcagc gaacttgtct ggaaacggac aactgcccac
     1201 tcggcatcaa agcgctgggc ggatgtgcat gacaccattc tattttacac aaggacttct
     1261 gattttatct gaaacaagac ttacactgag tatgaagaga aatacaaagc tcgctaccgc
     1321 aacgtcgatc ctgatggccg ccgctggatg gacgacaact tgaccgcacc ggggctacga
     1381 aatggtgatt caggagcaga ttggcgcagc ttcaatccct cggaaaaggg aaaccattgg
     1441 aaggtgcctg cgtcggagat tgtcgaactc atcggagcag aagaggctgc taaactgtcg
     1501 agcacccaaa agcttgaagt cctagggtca ggacccattg atctgcttga ggctggcgtc
     1561 gctgcgtgat tcaaggcccc cgagctgacg ggggtcatca tgagcaatct attctggctg
     1621 acggacgagc aggtggagcg gctgagaccg ttctttccga agagccatgg caagccgcgc
     1681 gtcgatgatc ggcgggtttt gagcggcata atcttcatca atcgcaatgg tttgaggtgg
     1741 tgcgatgcgc ccaaggagta tggcccgtcg aagacgctct acaaccgatg gaagcggtgg
     1801 ggggacatgg gtgtctttgc ccggatgatg gagggcctgg cctccgaggg caccgagcag
     1861 aagaccatca tgatcgacgc gacctatctg aaggcgcacc gcacggcttc gagcctgcgg
     1921 gcgaaaaagg ggggcccgaa gatcagcgcg ggcgcctgat cgggcgcacg aagggtgggt
     1981 tgaacaccaa gttgcatgcc gtcaccgatg cgaaggggcg tccgctgaag ttcttcatga
     2041 ccgcaggcca agtcagcgac tacactggcg ctgcagccct gctgggcagt ctgcccgctg
     2101 ctgaatggat gctcgctgac cggggctatg acgccgactg gttccgggat gcgttgaaag
     2161 acaaggggat acgcccctgc atccccggtc ggaagtcgcg cggcaagaca gtccgctacg
     2221 acaaaagacg ttaccggcgc cgcaaccgga tcgagatcat gttcggccga ttgaaagact
     2281 ggcgccgcgt tgcaactcgt tacgaccgat gcgcaaagac cttcctctcc gctgtcgccc
     2341 tcgccgcaac tgtcattttc tggctctgaa gatcaatgag tctggaccct aggctgttga
     2401 aagcaataag caacttaggg ggcgagtata tcaatccgaa ta
//
LOCUS       DAORVN010000306         2439 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_42649_length_2439_cov_4.703440, whole genome shotgun sequence.
ACCESSION   DAORVN010000306 DAORVN010000000
VERSION     DAORVN010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42649_length_2439_cov_4.703440"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(257..712)
                     /locus_tag="PKV23_06830"
                     /pseudo
     CDS             complement(257..712)
                     /locus_tag="PKV23_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007197648.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
     gene            complement(870..1559)
                     /locus_tag="PKV23_06835"
     CDS             complement(870..1559)
                     /locus_tag="PKV23_06835"
                     /inference="COORDINATES: protein motif:HMM:NF013460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HQX85185.1"
                     /translation="MQNKRIRLWDLPTRLFHWILLLAVAASVASGQVGGNWMDWHGRI
                     GLLVVGLLAFRIVWGVLGSTYARFAQFFPTPGRLKAYLRGEWRGEGHNPLGALSVFGL
                     LGLLTAQVTTGLFANDDIAFTGPLFDLVTKSLSNRLTGIHHLLTNVLYVLVALHVGAI
                     MFYAHAKKKNLVRSMVTGWMDDGEGESARGGGFVAFIVALLVAVAVVYYTSGAWLPKP
                     PPPVAVETPNF"
     gene            complement(1635..2090)
                     /locus_tag="PKV23_06840"
     CDS             complement(1635..2090)
                     /locus_tag="PKV23_06840"
                     /inference="COORDINATES: protein motif:HMM:NF013487.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HQX85186.1"
                     /translation="MKSKLLLALLALALTGTATAQLKPEEAIKFRQSGYAFMAWNMGR
                     IKANVEGQFNKEEVIKSANAIQAIANSGMGALYLPGTDKGTGWEKTRVKPAFFTDKEG
                     VTKVAMAFNKEANEMAKVAATGDVAAIKEQFGKLGETCKGCHDDFKAKK"
     gene            complement(2204..>2439)
                     /locus_tag="PKV23_06845"
     CDS             complement(2204..>2439)
                     /locus_tag="PKV23_06845"
                     /inference="COORDINATES: protein motif:HMM:NF021591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2322 family protein"
                     /protein_id="HQX85187.1"
                     /translation="VATIENKPGSQGSLAVYNHLAQTYGSINLEAAKKGLEIFAEHTD
                     DARGNPGKHPNIDRLIAIEQGGSALRVKHLFAV"
BASE COUNT          491 a          702 c          760 g          486 t
ORIGIN      
        1 atcgaaggcg ccaacgagga cggcgcggta gtctgagcgt tgctgcggtc gacacgaaaa
       61 agggagccaa attggctccc tttttttgtc gggaagcagc cgccttgcca aaaaatggcg
      121 gccacccacc agggcggccg ccaggcgaag ctgctgtcga tcaaccgccg gtgtaaagag
      181 agccggccgg ataataggat tgcggaccac ctgcagcatg gtgggtcgaa cgcgagtacg
      241 gtgtcgccga catcttctgc ggcggcatga tgaccgcgtc gattacatgg atcacgccgt
      301 tgctcgcgac gacgtcggtc ttgatgatgc gcgcgttgtc gatgcgcggg ccaccgtcgg
      361 tcgtgatctt cgccgactgg ccttcgacgg tcttgaccgg tccggccttg acatccttgg
      421 ccatgacctt gccggaaacc acatgatagg tcagcacgtc ggtcagtttc tttttgtccg
      481 ccagaagagc gttcaattgg tccttgggaa tctttgcgaa ggcctcgtcg gtcggcgcga
      541 agaccgtgaa ggggccgggg cccttcagcg tttcaaccaa gccagcggcc ttgacggccg
      601 tcaccagggt gttgaaggaa ccggccgaga ctgcagtgtc aacgatgtcg gccgccatgg
      661 cggagccact gaaagccagt gcaagaacaa gtgcggaagc agtttttttc atgacgatct
      721 cctgaacagt gtgggggtta gggtgcgccg aaatgatcgg ctgcactgct ctgagtcaac
      781 gctggacaga aagttcattt tgtctaggac aaactaagtg aaaagcacaa gacgtcacgc
      841 catgaggcat gccaggaatc aggcgaaaac tagaaattcg gcgtttcgac ggccaccggc
      901 gggggtggct tcggtagcca tgcgccactt gtgtagtaga caaccgcgac tgcaacgagc
      961 agggccacaa tgaacgcgac gaacccgccg ccgcgcgccg actcgccctc gccatcatcc
     1021 atccagccgg tgaccatgga gcgcacaagg ttcttctttt tggcgtgggc gtagaacatg
     1081 atcgccccga catggagtgc gaccaataca taaagtacgt tggtgagcag gtggtggatg
     1141 ccggtcagcc gattactgag gctcttcgtg accaggtcga atagcggccc ggtgaaggca
     1201 atgtcgtcat tggcgaacag cccggtcgtt acctgcgcgg tcaacaggcc gagcaggccg
     1261 aaaaccgata gggcgccgag tgggttgtgt ccttcgccac gccactcacc acgcaggtag
     1321 gccttgagcc tgccgggcgt cgggaagaac tgggcgaaac gggcataggt cgaaccgagc
     1381 acaccccaga cgatgcggaa cgcgagcagc ccgacgacca gcaggccgat ccgcccgtgc
     1441 cagtccatcc agttgccgcc gacctggccg ctggccaccg aggctgccac ggccagcaag
     1501 aggatccagt ggaagagtcg ggtaggtagg tcccacaggc gaattcgctt attttgcatg
     1561 ctcgattccg ttatcgaccc aaaaacgaat caggcgcccg gaggcgcctg cgaaagctct
     1621 tgcaggcctg acgcttactt cttggccttg aaatcgtcgt ggcagccctt gcaggtctcg
     1681 cccagcttgc cgaactgttc cttgatcgcc gccacatcac ccgtagccgc gaccttggcc
     1741 atttcattgg cttccttgtt gaacgccatg gcgaccttgg tgacgccttc cttgtcggtg
     1801 aagaaggccg gcttgacgcg ggtcttttcc cagcccgtgc ccttgtcggt gcccggcagg
     1861 tagagggcac ccatgccgga attggcgatg gcctggatgg cgttggcgga cttgatgact
     1921 tcttccttgt tgaactggcc ttcgacgttg gccttgatgc gacccatgtt ccaggccatg
     1981 aaggcatagc cggactggcg gaacttgatc gcctcctccg gcttgagttg ggcagtagcg
     2041 gtaccggtca gcgcaagggc cagcagggca agcagaagtt tggatttcat ggcatttctc
     2101 cgtcgttatt gaggaattcc gggatacgaa atgtgaacgc cggaacccgg ccattcaccg
     2161 ctgctatatt ataaccatct aatatgacaa ccggacgaag tactcagacg gcaaagaggt
     2221 gcttgacgcg cagggccgac ccgccctgct cgatggcgat cagacggtcg atgttgggat
     2281 gcttgcccgg gttgccgcgg gcatcgtcgg tatgctcggc gaaaatttcc agccccttct
     2341 tcgcggcttc gaggttgatc gaaccgtagg tctgcgccag atggttgtag acggccagcg
     2401 agccctggct gcccggcttg ttttcgatgg tggcaacca
//
LOCUS       DAORVN010000307         2436 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_42739_length_2436_cov_3.454011, whole genome shotgun sequence.
ACCESSION   DAORVN010000307 DAORVN010000000
VERSION     DAORVN010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42739_length_2436_cov_3.454011"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..473
                     /locus_tag="PKV23_06850"
     CDS             <1..473
                     /locus_tag="PKV23_06850"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HQX85188.1"
                     /translation="LWQIPGCRFVNGWLLDTNVVSELRKPKPSKLVLDWMRATGSANL
                     FLSSITFAEIRFGIEMQHDPDLRSRLAIWLDTVIRPMFEGRVVEANEDVLLEWRLMGE
                     ALSRQKRTVPEPDLLLSAIARHHRLNMASRDVKHIKVTGIPVLNPWTGERFNGA"
     gene            complement(470..955)
                     /locus_tag="PKV23_06855"
     CDS             complement(470..955)
                     /locus_tag="PKV23_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HQX85189.1"
                     /translation="MLRDPATWVAVAFILFIALGIYLKVPAMLAKMLDERSDKISKEL
                     AEARKLREEAQALLAEYQKKRAEAEKDAANIVAQAKVEAESYGVETRKKLAETIERRT
                     KQAAQKIAQAEAAAIKEVRTTATETAIAAASRLVGEAVQGAAGSKLIDESIAAVKSRL
                     N"
     gene            complement(965..1450)
                     /locus_tag="PKV23_06860"
     CDS             complement(965..1450)
                     /locus_tag="PKV23_06860"
                     /inference="COORDINATES: protein motif:HMM:NF012645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B'"
                     /protein_id="HQX85190.1"
                     /translation="MPQLDMSTWPPQLFWLAVTFLALYFVISRVAIPRTGGVIALRKS
                     TIDGDLAKAKNLKDETENAVRSYESALADAKAKAQAINLENRNKLNAEIEAERAKLDA
                     ALGAKIATAEKKVAASRDKALEDISAMAADIASDIVQQLTGSKITKAAASAAVSKSLG
                     K"
     gene            complement(1521..1745)
                     /locus_tag="PKV23_06865"
     CDS             complement(1521..1745)
                     /locus_tag="PKV23_06865"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006206606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HQX85191.1"
                     /translation="MEVEAAKMIGAGIAAIALGGAGVGIGTIFGNYLAGALRNPAAAA
                     SQRGTLFLGFAFAEATGLIGFAVAMLILFG"
     gene            complement(1776..>2436)
                     /locus_tag="PKV23_06870"
     CDS             complement(1776..>2436)
                     /locus_tag="PKV23_06870"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HQX85192.1"
                     /translation="DISFTNSALWAVIAVTCASLLFILAPRNLIPTRLQSVAESIYEV
                     VDNMTHEVLHENARTYFPFVLTLFVFILFANVLGLIPYSFTVTSHIVITLALALVVFI
                     GATIIGFIRNGFGYLKLFVPSGVPTLLLPLVVLIEIVSYFIRPMSLSIRLFANMMAGH
                     MMLKVMAGFVVMLGVTAGWLPLVAMVGLMGLELLVAALQAYVFALLTCMYLSDAMHVD
                     H"
BASE COUNT          503 a          680 c          754 g          499 t
ORIGIN      
        1 acctttggca aattccggga tgtcgatttg tgaacggctg gctccttgat accaatgttg
       61 tctctgaatt gcggaagccc aagcccagca agctggtact cgactggatg cgggcgaccg
      121 gaagcgcaaa tctttttctg tcatcgataa ctttcgcgga aattaggttt ggcatcgaaa
      181 tgcagcacga ccccgacctc aggtcgcggc tcgctatctg gctggatacg gttatccgcc
      241 caatgttcga aggacgcgtt gttgaagcga atgaggatgt tctcctcgaa tggcggctaa
      301 tgggcgaggc actgtccaga cagaaacgaa ctgtgccgga acccgacttg ctgctcagcg
      361 caattgccag gcatcatcga ttgaacatgg cgagccgcga cgtcaagcac ataaaagtaa
      421 cgggtattcc tgtcctcaat ccctggactg gtgagcgctt taacggggcc taattcagcc
      481 ggctcttcac cgcggcgatg gactcgtcga tgagcttgga gcccgcagca ccctgaaccg
      541 cttcgccaac cagcctggag gcggccgcga tggcggtttc ggttgccgtg gtgcgcactt
      601 ccttgatggc agcggcctcg gcctgggcga ttttctgggc tgcctgtttg gtacggcgct
      661 cgatggtttc cgccagcttc ttgcgggttt caaccccgta ggattcagct tccaccttgg
      721 cctgggccac gatattggct gcgtcctttt cggcctcggc acgcttcttc tggtactcgg
      781 ccagaagcgc ctgggcttct tcacgcagct tcctggcttc cgccagttcc ttgctgatct
      841 tgtcggaccg ctcgtccagc atcttggcca gcatcgccgg caccttgaga tagatgccaa
      901 gcgcgatgaa gaggatgaag gcgacggcaa cccaggttgc aggatcacgt aacatggctt
      961 gctcctattt tcccagggac ttggaaacgg cggcgctggc cgcggccttg gtgatcttgc
     1021 tgcccgtcag ctgctggaca atgtcgctgg cgatatcagc cgccatggcg gagatatctt
     1081 ccagcgcctt gtcgcgcgaa gcggcaacct tcttttccgc cgtggcaatc ttggcaccaa
     1141 gggccgcatc aagcttggcc cgctccgcct cgatttcggc attgagcttg ttgcggtttt
     1201 caaggttgat ggcctgggcc tttgccttgg catcagccaa tgcggactca taggagcgca
     1261 ccgcgttttc ggtttcgtcc ttgagatttt tggccttcgc cagatcgccg tcaatggtcg
     1321 acttgcgaag tgctatcacg ccccccgtgc gcgggatggc cacgcgcgag atgacgaaat
     1381 agagcgccag gaaagtgacg gcgagccaga aaagctgtgg cggccaggta gacatatcaa
     1441 gctgcggcat gggcaggccc tgtgaatgcg tttgaacggc gccgcaccat gcagcgccgg
     1501 ccagaggtca gcggctggta tcagccgaac agaatgagca tggcaacggc gaagccgatg
     1561 aggcctgttg cttcggcgaa ggcaaagccg aggaacaggg tgccgcgctg gctggcggcg
     1621 gcggccgggt tgcgcaaggc gcctgccagg tagttgccga aaattgtgcc gataccaacg
     1681 ccagcgccac cgagggcaat agctgcaata cctgcaccga tcatctttgc tgcttcaact
     1741 tccatttttt tctcctgtgg aatggatggg tttagttagt ggtcaacatg catggcatcg
     1801 ctgagataca tgcaggtcaa gagtgcgaaa acataggcct gaagggcggc cacgagcagt
     1861 tcaaggccca tcaggcccac catggcgacc agcggcagcc agcctgccgt gacgccgagc
     1921 atgacgacga agcccgccat gactttcagc atcatgtggc ccgccatcat gttggcgaaa
     1981 agccggatgg aaaggctcat cgggcggatg aaataggata cgatttcgat gagcaccacg
     2041 agggggagca gcagggtggg cacgcctgaa ggcacgaaca gcttgagata gccgaagccg
     2101 ttgcggatga agccgataat cgtggccccg atgaacacca cgagcgccag cgccagggtg
     2161 atcacgatat ggctggtcac cgtgaaggag tagggaatga ggcccagcac gttggcaaag
     2221 agaatgaaga cgaacagcgt cagcacgaaa gggaaatagg tgcgggcgtt ttcatgcagc
     2281 acttcgtggg tcatgttgtc gaccacttcg taaatgcttt cggccaccga ttgcagccgc
     2341 gtcgggatca ggttgcgcgg cgccaggatg aacagcagcg aggcgcaggt aaccgcgatc
     2401 accgcccaaa gggccgaatt ggtgaaggaa atgtcg
//
LOCUS       DAORVN010000308         2427 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_43031_length_2427_cov_2.963322, whole genome shotgun sequence.
ACCESSION   DAORVN010000308 DAORVN010000000
VERSION     DAORVN010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2427)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2427)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2427
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43031_length_2427_cov_2.963322"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(159..401)
                     /locus_tag="PKV23_06875"
     CDS             complement(159..401)
                     /locus_tag="PKV23_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018428959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1344 domain-containing protein"
                     /protein_id="HQX85193.1"
                     /translation="MNKFVALLIASAAMVGTAYAGQIQGTVKAFDEATKTVTLEDGSA
                     YVLAAGVMPTGITAGAKVQVTFDDTSKAATAVTVVQ"
     gene            complement(606..2186)
                     /locus_tag="PKV23_06880"
     CDS             complement(606..2186)
                     /locus_tag="PKV23_06880"
                     /inference="COORDINATES: protein motif:HMM:NF017085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HQX85194.1"
                     /translation="MSGSAAGGPSPNGGASKLEGQEIEIKFRTDAAGLALLLDAPLLN
                     AATDLQTETLKATYFDTPSHGLRKKGIILRIRKSGDDEPVLGMKGPGAASDGPFHRKD
                     VEVICSGQKPDLALFDKATKRLLERTIGDQPLAAKFKMEFKRLSGLVTSGSSVVEVAV
                     DQGSVTAGKQRLPLAEVELELVSGCKADLLDLAMRLSKDFALRLDFVSKAEKGFRTLL
                     AEKPALAKAVPLKSKSLATFDDAVTAILSNTLAHFVANWAGLRESDEPESIHQMRIAL
                     RRMRCGLAILNRAAPGAGFGELRDDAGKLATAFGPARNADALRLSLLQGALASMDCPE
                     SRDALRAMLDKRRVAAYAAARAQLDGPAGTEFVLKVQGVLARRGWRETPGSSGPKISK
                     APARKLFRAELNRLRSRVLKREKAAGVSDQGRHKLRIALKNLRYGVDFFAGLFGSAKS
                     RRAYAKRLADLQDLLGLRNDIVVARVYLKELRDEMGPEADRILEFIRGWYAREAEGAD
                     KAIGKSWKKFQREEIFWT"
BASE COUNT          498 a          751 c          678 g          500 t
ORIGIN      
        1 ctgggaaaaa atgttccttc acaaaccggg cagcccgccg ccgccatatg taggccctgg
       61 ccggggaaaa atccagtgaa tcaaatgaaa acacgcccgg cagcggggcc gggcgcgcaa
      121 atatcactaa tggacgctta aattcgtccg gtgtgaaact actggacgac cgtaacggca
      181 gtcgccgcct tgctcgtgtc atcgaaagtt acctgcacct tggctccggc agtgatccct
      241 gtcggcatga cgccggcagc cagaacatag gctgagccgt cctcaagggt tacagtcttt
      301 gtggcctcgt caaacgcctt gaccgtgccc tggatctggc cggcataggc ggttccaacc
      361 atcgcggcgg aagcaataag caatgctaca aacttattca tttcacattt ccttcgtttt
      421 gacgcccgta cgccgaaaac gggcaatttg aattcccggg cggatcaatg tccagtccgg
      481 cgtccgcccg cgaaacacgc gcggacacct cctaggtagg caaagccgct gcgccggaac
      541 acttaagaat acttaatttt catcggcaca tttttgccgg atttcatggc gcggcgcggg
      601 gcaaatcagg tccagaaaat ttcttctcgc tgaaattttt tccatgactt gccgattgcc
      661 ttgtcggcac cttcggcctc gcgggcatac cagccccgga tgaattccag gatccggtcc
      721 gcctctgggc ccatctcgtc cctgagctct ttcaagtaga ccctggcaac gacaatatca
      781 ttgcgcaggc cgagaagatc ctgcaggtcg gccagccgtt tcgcgtaagc tcgcctgctc
      841 ttggcgctgc caaacaaacc ggcaaaaaaa tccacgccat agcgcaggtt cttgagggcg
      901 atgcgcaatt tgtgccggcc ttggtcagaa acgccggcgg ccttctcgcg cttcaacacg
      961 cgcgacctca gccggttaag ctccgccctg aacaatttcc gcgcaggcgc tttggatatt
     1021 tttgggccgc tacttcccgg agtctctcgc cagccgcggc gtgccaggac gccttgcacc
     1081 ttcagcacga attctgtccc ggcagggcca tccagttggg cccgcgccgc agcgtaagcc
     1141 gcaacccgcc gcttatcgag catggcgcgc aacgcatcgc ggctttcagg gcaatccatg
     1201 cttgccaggg caccctgcag cagtgacagg cgaagggcat ccgcgttccg cgccggacca
     1261 aatgctgttg ccagtttgcc ggcatcgtca cgcagttcgc caaatccggc gccgggcgcg
     1321 gcgcggttga gaatggccag gccgcagcgc atgcggcgca gcgcgatccg catctgatga
     1381 atggattcag gctcgtcgct ttcccgcaaa ccggcccaat tcgccacgaa gtgagccagc
     1441 gtattggaaa ggatggctgt cactgcgtcg tcgaacgtcg cgagggattt tgatttcagt
     1501 ggcacggcct tggccagcgc gggcttttcc gcaagcaggg tgcggaatcc cttttccgcc
     1561 ttgctgacga aatcgaggcg caaggcaaag tctttcgaga gcctcatcgc caggtcgaga
     1621 aggtctgcct tgcacccaga caccagttca agttcgactt cggccaatgg caaacgctgc
     1681 tttccagcgg taacagagcc ctggtccact gcaacctcga caaccgaact gcctgaagta
     1741 acgaggccgc tcagccgctt gaactccatt ttgaattttg ctgcgagggg ctggtccccg
     1801 atggtccgtt ccaggagcct tttggtggct ttgtcaaaca gggcgagatc aggcttctgg
     1861 cctgaacaaa tgacttcaac gtccttgcgg tgaaagggcc cgtcgctggc cgcccccgga
     1921 cccttcatcc ccaacacggg ctcgtcatcg ccggacttgc ggatccgcag gatgatgcct
     1981 ttcttccgca agccatgcga aggtgtgtcg aaatacgtgg ccttcagggt ttcggtctgc
     2041 aggtcagttg cggcattgag cagtggagca tccaggagga gcgccaatcc ggcagcatcg
     2101 gtacgaaact tgatttcgat ttcctgaccc tcgagcttgg aggcaccccc gttggggctc
     2161 gggccgccag ccgctgatcc agacatctca cacccaccct tacacctgca tacctgccgc
     2221 aaacaccgga aggagtaacc gattattatt gatttaggcg gacgaatcaa cgccagttga
     2281 attctgagtc gcttgaatcg gaagaatgcg atgcaccaga ttgctgctct gccattttat
     2341 gacaacccgg gtggtggtgt ggacgtctgc ctcgtgaccg ccaggggcag cgggcgctgg
     2401 atcatcccaa aaggcaatcc catccgc
//
LOCUS       DAORVN010000309         2422 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_43178_length_2422_cov_4.790030, whole genome shotgun sequence.
ACCESSION   DAORVN010000309 DAORVN010000000
VERSION     DAORVN010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2422)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2422)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2422
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43178_length_2422_cov_4.790030"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..174)
                     /locus_tag="PKV23_06885"
     CDS             complement(<1..174)
                     /locus_tag="PKV23_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010400577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HQX85195.1"
                     /translation="MPGSGLTMKIGQLAQQTGTLVDTIRYYEREGLLPRAARTEGNYR
                     IYDAVHLERLAFIR"
     gene            267..>2422
                     /locus_tag="PKV23_06890"
     CDS             267..>2422
                     /locus_tag="PKV23_06890"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HQX85196.1"
                     /translation="MKPSPISLQPETQPSYCGCSGTCHSPEAPSTNVPLQADDTEGGL
                     QLRIPAMDCPTEESEIRRAVEHIGAIGGLRFDLSARTLRIDAPNEALPSIIDAIRKAG
                     FAAELVKPGTPAGTDRDGIPRGIAIFAMALVLALGAEVLAYLMPEVLLAKIAGMGLAA
                     TAIVMAGFSTYSKGLAALSQGRLNINALMSVAVTGAFAIGQWPEAAMVMALYSIAELI
                     EARSVDRARNAIKSLLALTPETAEVRQADGSWDVLASRDVMLAAVVRVKPGARIPLDG
                     VVTAGSSAVNQAPVTGESLPVDKTIGDTVFAGTINESGLLEFRVTARSTETTLARIIH
                     AVETAQGSKAPMQRFVDRFAALYTPAVFVIALAVALLTPWLLEWSWSQALYKALVLLV
                     IACPCALVIATPVTVVSGLASAARRGILIKGGVYLEDARKLKVIALDKTGTITEGKPK
                     RVATEILDQTVSEKVILEWAGSLAALSNHPVSKAISTALAPSATVVDHFMALPGRGVQ
                     GRIGNQDLILGNHRLIEERKLCGPAIEARLVEHEAQGRTMTLLANESGVLAIFAVADT
                     IKPHAREAISALHTLGVKAVMLSGDNQATAQAVAAQANVDDARGNLLPDDKLKAIEAL
                     QKEFGPTAMTGDGINDAPALARADIGIAMGAAGTDTAMEAADIVIMNDDLRRIPEAIR
                     LSRTTHRVLWQNIILALGIKAIFLVLALLGSATMWMA"
BASE COUNT          489 a          704 c          740 g          489 t
ORIGIN      
        1 ccgaatgaag gccagacgtt caagatgaac ggcatcgtag atccgatagt tgccttccgt
       61 tcgcgctgca cgcgggagca gcccctcgcg ctcatagtaa cgaatggtgt ctaccagagt
      121 acctgtctgc tgggcgagct gaccgatttt catcgtaagg cctgagcccg gcatgagctc
      181 aaaagtgaat tgacgaaggc gatcacctta cccccttgac tctggagttg ctacagggtt
      241 tccaatggca gcaataggag atcaccatga aaccatcgcc catcagcctg caacccgaga
      301 ctcagccctc atactgcggg tgtagcggca cgtgtcactc ccccgaggcg ccctccacga
      361 acgtgcctct gcaagccgac gatacggaag gcggtttgca gcttcgcatt ccggcaatgg
      421 actgtcctac ggaggagagt gagatccgcc gggcggtcga gcatatcggc gcgatcggtg
      481 ggcttcgttt tgatctgtcg gcccgcaccc tgcgcatcga tgcgccaaat gaagcgctgc
      541 caagcatcat cgacgccatc cgcaaagcgg gctttgccgc agagctcgtc aaaccgggta
      601 cgccagcggg cacagatcgc gacggcattc cacgtggtat cgctattttt gcgatggcgc
      661 tcgtcctggc gcttggcgca gaagtcctgg cttacctgat gcccgaagtg cttctagcca
      721 agattgccgg gatgggtttg gcggcaacag cgatcgtgat ggccggattt tcaacctata
      781 gcaaggggct tgctgccctg tctcaggggc gtctgaacat caatgccctg atgtcggttg
      841 cggtcacagg tgccttcgcc attggtcaat ggcctgaagc cgccatggtg atggctttgt
      901 attccattgc agagctgatt gaagcaaggt cggtagatcg ggcacggaac gcgatcaaga
      961 gcctgctcgc gctcacgccg gaaaccgctg aagtgagaca ggctgatggc agttgggatg
     1021 tgcttgcctc ccgcgacgtg atgcttgctg cagtcgtcag ggtcaagcct ggcgcacgca
     1081 ttccgttgga tggcgtcgtg actgccggga gcagtgcggt caatcaggca ccggtgaccg
     1141 gcgagagcct gccggtggac aagaccatcg gtgacacggt ctttgccggg acgatcaacg
     1201 aatcagggct gttggaattc agggtgacgg ctcgatccac tgagaccaca ctggcccgga
     1261 tcatccacgc ggttgaaacc gctcaaggca gcaaggcgcc tatgcagcgc tttgtcgatc
     1321 gctttgcagc cctctacacg cccgctgtgt tcgtgatcgc gctggccgta gcgctcctga
     1381 ccccgtggtt gttggagtgg agctggtctc aagcgcttta caaagccttg gtccttcttg
     1441 tgatcgcctg tccctgtgcc ttggtgattg caacgcccgt tacggtagtg agtgggctcg
     1501 ccagcgcagc acgtcggggg attttgatca aaggcggcgt ctatctggag gatgcccgta
     1561 agctgaaggt cattgctctc gacaagacgg ggacgatcac cgaaggcaaa ccgaagcggg
     1621 tcgcaaccga aatcctggat cagaccgtat ccgagaaagt gattttggag tgggccggca
     1681 gcctggcagc cctctccaac caccctgtat caaaagccat ttcaacggca ctggcgccgt
     1741 ccgccaccgt tgtcgatcac ttcatggcgc tgccggggcg tggtgttcaa ggtcgcatcg
     1801 gtaaccaaga cctgatcctt gggaatcatc gattgatcga ggagcgcaaa ctgtgcggtc
     1861 ctgccatcga agctcgcctc gtcgaacatg aggcccaggg gcgcacgatg acactcctgg
     1921 ccaacgaaag cggagtgctg gcgatcttcg ccgttgccga cacgatcaaa cctcatgccc
     1981 gggaagccat ctcggccttg cataccctgg gcgtcaaggc agtcatgctg tctggcgaca
     2041 accaggcaac ggctcaggcc gttgccgcac aggctaatgt ggacgatgca cgcgggaatc
     2101 tgctgcctga cgacaagctc aaggccatcg aagccttgca gaaagaattt ggccccacgg
     2161 cgatgaccgg tgacgggatc aacgatgcgc ctgcgttggc acgcgccgat atcggcatag
     2221 caatgggggc tgcggggacg gataccgcca tggaagccgc cgacatcgtg atcatgaacg
     2281 atgatctgcg ccgtatccct gaagccattc ggctctcgcg cacgacgcac agggtgcttt
     2341 ggcagaacat cattctggcg ctgggaatca aggcaatctt cctggttctg gctctgctag
     2401 gcagtgccac catgtggatg gc
//
LOCUS       DAORVN010000310         2421 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_43218_length_2421_cov_3.600592, whole genome shotgun sequence.
ACCESSION   DAORVN010000310 DAORVN010000000
VERSION     DAORVN010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2421)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2421)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2421
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43218_length_2421_cov_3.600592"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(162..821)
                     /locus_tag="PKV23_06895"
     CDS             complement(162..821)
                     /locus_tag="PKV23_06895"
                     /inference="COORDINATES: protein motif:HMM:NF013180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HQX85197.1"
                     /translation="MAGTDGLPPGRDASGTLKDEVAGTVPDSPENGTSFPAEAPSTAE
                     PPLAEHFRNLNLVERDPVTGLANRRGFAIQMAELLLSDMPRCPGALVLLEFTNFNEYN
                     IVFGSRRGDELLKLAGDIVSKIWNNHLMVRARIGEATFALGMGDLTLEEVQAFGQMLH
                     HSLALRLSGEDFSGYADFRCGGAYFQRKGTLPALLVTAKQAISQSRNRLENVFELLQC
                     G"
     gene            complement(909..>2421)
                     /locus_tag="PKV23_06900"
     CDS             complement(909..>2421)
                     /locus_tag="PKV23_06900"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HQX85198.1"
                     /translation="DEMSSLWCRMGQKITGIDELLGSGSKNQQYGAQESVKRFLGRAG
                     EKEWEEMLEPATLRDVLYVNPQPSCLGLFPALRASGWEPHAVTTSEQFRRLTRSRECN
                     VGILRIEKQSDLGTFPNLSELLGEQNREWVGLLTKEAVNDPEVRRLIAECFFDFHTLP
                     LSPEHITTTLGHAYGMASIKRLDLEKSLGKLEEAEMVGCSPVMLGLFRNIRKVAGTEA
                     AVFITGETGTGKELTAQAIHERSSRAGGPFVTINCGAIPSSLIQSELFGYEKGAFTGA
                     MQRKIGRIEAASGGTIFLDEIGDLPMDLQANLLRFLQEKQIDRVGGKDPVTVDVRVIA
                     ATHVDLEMAVAAGRFRQDLYHRLNVLGLRVPSLRERSGDIEVLARHFFTQFSDGRRRS
                     LRGFSREAMEAMTKHDWPGNVRELINRVRRAVVMCEGRTISSGDMGFEAVSSRHQMLT
                     LDETRNQAEQEAILSAMNRNVNNLARTARDLGVSRVTLYRLIDKHQLRGHSEE"
BASE COUNT          490 a          738 c          698 g          495 t
ORIGIN      
        1 ttggcatctc agactggatc gatcccgaca cgggcgcgcc ggcggaggaa ggtgcaccgc
       61 atatagaaga tcactgaaca gcactcagtg aagtggtagc cgaaacctct ctagggcctg
      121 ttgacattca tttgatcgga ccaaatgaat gtcaacaggc cctagccgca ttgcagcagt
      181 tcaaaaacat tctcaagtcg gttccggctt tgggaaattg cctgctttgc cgtgacaagc
      241 agcgccggca gcgtcccctt tcgctggaaa taggcgccgc cgcaccggaa atccgcgtag
      301 cctgagaagt cctcgccgga cagccgcaat gccaggctat ggtgcagcat ctggccaaac
      361 gcttgtacct cctccagcgt taaatcaccc atcccaagcg cgaaggtggc ctcgccgatg
      421 cgggcgcgca ccatcaggtg attgttccag attttggaaa cgatatcacc tgccagcttg
      481 agcaactcat ctcccctgcg cgatccgaat acgatgttgt actcgttgaa attggtgaat
      541 tcgaggagca caagcgctcc cgggcagcgc ggcatgtcgg aaaggaggag ttccgccatc
      601 tggatcgcga agccgcggcg attggcaaga cccgtgacgg gatcgcgctc tacaagattg
      661 agattcctga agtgctcggc aagcggcggt tccgcagtgg aaggggcttc cgcagggaac
      721 gaggtgccat tttccgggct gtccgggacg gtgcccgcca cctcatcctt cagggtgccg
      781 gaggcatcgc gccccggcgg aagcccatca gtcccagcca tgatgcactc cgtagcgcgg
      841 gcggcctcgg aaaaccgccc gttcggagaa atattgattg gcgcttttca ctcttcaacc
      901 gcctgacatt actcttcgct atgcccacgc aactgatgct tatcgatcag ccgatacaaa
      961 gtcacgcgtg aaacgcccag atccctggct gtgcgcgcca gattatttac gttgcggttc
     1021 atggccgaca gaatcgcttc ctgctctgcc tgattcctcg tctcgtcgag ggtcagcatc
     1081 tggtgccggg aggaaaccgc ctcaaagccc atgtcccccg atgaaatcgt gcggccctcg
     1141 cacatgacca cggcgcggcg cacgcggttg atgagttcgc ggacattgcc gggccaatcg
     1201 tgcttggtca ttgcctccat ggcttcgcgg gagaaaccgc gcagggagcg gcggcggccg
     1261 tcggaaaact gggtgaagaa gtggcgcgcc agcacttcga tgtcgccact cctctcgcgc
     1321 agggagggaa cgcgcaggcc gagcacgttc agccggtggt aaaggtcctg cctgaaccgt
     1381 cccgcggcga cggccatttc aagatcgacg tgggttgcgg cgatgacgcg cacatccact
     1441 gtcaccggat ccttgccgcc gacccgatcg atctgcttct cctgcaggaa ccgcagtaag
     1501 ttggcctgga gatccatcgg caggtcgccg atttcatcca ggaagatcgt gccgccggaa
     1561 gccgcctcga ttcttccgat cttgcgctgc atcgctccgg taaaggcgcc cttctcgtaa
     1621 ccgaacaact ccgactggat caggctggaa ggaatggcgc cgcagttgat cgtcacgaaa
     1681 ggaccgccgg cacgggagga acgctcgtgg attgcctggg ccgtgagttc ctttccggtg
     1741 ccggtctcgc cggtgatgaa gacggccgct tccgtgccgg ccactttccg gatgttgcga
     1801 aagagcccca gcatgaccgg gctgcaaccg accatctcgg cttcctccag tttgcccagg
     1861 gatttctcca ggtcgagtcg cttgatcgaa gccatgccat aggcgtggcc gagcgtcgtc
     1921 gttatgtgct cgggactgag gggcaaggtg tgaaaatcaa aaaaacactc tgcgatcagc
     1981 cgccgtacct cggggtcatt cactgcttcc ttcgtcagaa ggccgaccca ttcgcggttc
     2041 tgttcgccaa gcaattcact gaggttggga aacgtcccca gatcggactg tttctcgata
     2101 cgcagaatgc cgacgttgca ttcccgcgat cgggtcagcc tgcggaactg ttcagaagtg
     2161 gtgactgcat gcggttccca gcctgacgcg cgcagtgcag gaaacagtcc aagacatgac
     2221 ggttgcgggt taacatagag cacatcccgc aacgttgcag gttccagcat ttcttcccac
     2281 tccttctctc cagcgcgtcc gagaaatcgt ttcacggatt cctgcgcgcc atattgttga
     2341 ttttttgagc cagagccaag cagctcatca atgcccgtta ttttttgacc catccggcac
     2401 cacagcgacg acatttcatc g
//
LOCUS       DAORVN010000311         2418 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_43344_length_2418_cov_2.812526, whole genome shotgun sequence.
ACCESSION   DAORVN010000311 DAORVN010000000
VERSION     DAORVN010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2418
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43344_length_2418_cov_2.812526"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..853
                     /locus_tag="PKV23_06905"
     CDS             <1..853
                     /locus_tag="PKV23_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019963891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HQX85199.1"
                     /translation="GYLAGETPIPCVKRNQTVKFADLLQRARDLDAAALVTGHYIESR
                     ARGDGSGHRDLFTPADMARDQSYFLFATTQEQVDFLRFPLGALPKAETRRIAAELGLV
                     VADKPDSQDICFVPHGRYADVILRLRPDAAEPGDILHLDGRILGRHEGIIHYTIGQRR
                     GLGIAEAEPLYVVKVDAARKQVVVGPREALKQSDIRLASLNWLGQKALNGRVQPVFAK
                     IRSTRPPVEAALRDGPEGSIVTIVDGEYGVSPGQACVLYDGQGPGARVLGGGFIASSA
                     IRDSAIG"
     gene            850..1455
                     /locus_tag="PKV23_06910"
     CDS             850..1455
                     /locus_tag="PKV23_06910"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HQX85200.1"
                     /translation="MTGPLTSAIKASKRSKLFFLKQYLRNPFGTGGVAPSGRQLAKLM
                     VAKLAPQPSEIIVELGPGTGSFTRELLAQGVEPANLILVEFNKAFVKFLKKEFPNLRI
                     VEGAAQDLPQLLTGLGQFSVNKIISGIPLRSMKPAECRQIARAVAAVLEPGGLFVQFS
                     YFKASPVPRAVAAEVGLVGKCVGGVLNNVPPAFVWQYVKSA"
     gene            1515..2318
                     /locus_tag="PKV23_06915"
     CDS             1515..2318
                     /locus_tag="PKV23_06915"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HQX85201.1"
                     /translation="MAKRVIKKPNIDLEVKKGIAWAVHAFTTCGIVAGFLALVEVLNN
                     DPVKAFMWLGIALFVDGIDGSLARRARVREYTPNFDGGSLDYVIDFFTYVAVPAVMVY
                     WFEMVPLEWIFAGTTWSLIAAATIMAVSCYTFANVGMKSEDYYFVGFPAIWNVVVLYF
                     YVLDTGWLVNGLTVIILSILTFIPIKFVHPLRVAHWRNITVPMTVLWAAMALSLVISS
                     KDKGPTEAMYKIQLTVWIGASLYFAWISLWRTFVQGDPGPADETPDHTT"
BASE COUNT          458 a          672 c          773 g          515 t
ORIGIN      
        1 gggctatctg gccggggaaa cgccgattcc gtgcgtgaag cgtaaccaga ccgttaaatt
       61 cgccgatctg ctgcagcggg ccagggacct ggacgcggcg gcgctggtga ccggccatta
      121 tattgaaagc cgggcgcgag gcgatggttc ggggcaccgc gatcttttta cacctgccga
      181 catggcgcgc gaccagagct acttcctgtt tgccaccacg caggagcagg tggattttct
      241 gcgctttccc ctgggcgcac tgcccaaggc cgagacacgg cgcattgccg ccgaacttgg
      301 tctcgtcgtg gcggacaagc cggacagcca ggatatctgc ttcgtgccac acggccggta
      361 tgccgatgtg attttgcggc tgcgcccgga tgcggccgag cctggcgaca ttctgcatct
      421 ggatgggcgg atcctcggcc ggcatgaagg catcattcat tacacgatcg ggcagcggcg
      481 cgggcttggc atcgcagagg cggaaccgct ttacgtggtg aaggtggatg cggcgcgaaa
      541 gcaggtggtg gtgggaccgc gggaggcgct gaagcagagc gacatccgtc tggccagcct
      601 caactggctt gggcagaaag cgttgaatgg cagggtccag ccggttttcg ccaagatcag
      661 gtccacgcgc ccgccggttg aagccgcctt gcgggacggg cccgaaggtt ccatcgttac
      721 aatagtcgac ggcgaatacg gcgtttcgcc ggggcaggcc tgcgtgcttt atgacgggca
      781 ggggccgggt gcgcgggttc tgggtggcgg cttcatcgcc tcttcggcga tccgggacag
      841 cgccatcgga tgaccgggcc cctgacatct gccatcaagg ccagcaagcg ttcaaaactt
      901 tttttcctga agcaatacct gcgcaatccc ttcggcaccg gcggcgtggc gcccagcggc
      961 cggcaactgg cgaagctcat ggttgcgaaa cttgccccgc agccgtcaga gatcatcgtg
     1021 gaattggggc cggggactgg ctcgttcacg cgggaactcc tggcgcaagg tgttgaaccg
     1081 gccaacctca tcctggtgga attcaacaag gcgtttgtca aattcctcaa gaaggaattt
     1141 ccaaacctgc gcattgtcga gggagcggcg caggatctgc cgcaattgct gacaggcctg
     1201 ggacagtttt cggtgaacaa gatcatctcc ggcattccgc tgcgcagcat gaaaccggcg
     1261 gaatgccggc aaatcgccag ggcggtcgcc gccgtgcttg aacccggggg actgttcgtg
     1321 cagttcagct atttcaaggc ttcgccggtg ccgagagcgg tggcagcaga ggtgggactg
     1381 gtcgggaaat gcgtgggcgg cgtgctgaac aatgtcccgc cggcctttgt ctggcagtat
     1441 gtaaagtccg cataggcttc ttgccccaaa cttggcgcac ccctatagtt tgaatcgcag
     1501 ggagaagctg tttagtggcc aagcgggtaa tcaagaagcc aaacattgat ctcgaagtga
     1561 agaaaggcat cgcctgggcg gtacatgcct ttacaacctg cggaattgtt gcaggctttc
     1621 tggcgcttgt ggaagtgctg aacaacgacc cggtcaaagc cttcatgtgg ctgggcattg
     1681 cgcttttcgt ggacggcatt gacggttcac tggcccgccg cgcaagggtg cgtgaataca
     1741 cgcccaattt cgatggcggc tcgcttgact acgtcattga ttttttcacc tatgtggcgg
     1801 tgcccgccgt gatggtctac tggttcgaaa tggtgccgct ggaatggatt ttcgccggaa
     1861 ccacctggag cctcatcgcg gcggccacca tcatggccgt gtcgtgctac actttcgcca
     1921 atgtcggcat gaagtctgaa gactattatt ttgtgggctt cccggcgatc tggaacgtgg
     1981 tggtactgta tttctacgtt ctcgacacgg gctggctggt gaatggcctc accgtcatca
     2041 tcctttccat attgacgttt attcccatca aattcgtgca tccgctgcgg gtggcgcact
     2101 ggcgcaatat aacggttccg atgacagttc tttgggccgc catggccctg agcctggtga
     2161 tcagcagcaa ggacaagggc ccaaccgagg ccatgtacaa gatccagctc acggtctgga
     2221 ttggcgcttc gctctatttc gcctggattt ccctgtggcg gaccttcgtt cagggcgacc
     2281 cgggcccggc ggatgaaacg ccggaccaca caacttgact tgaggtgcga taacttccta
     2341 taagccgacg cagatttggc gaggtagctc agttggttag agcacgggaa tcataatcct
     2401 ggggtcgggg gttcaagt
//
LOCUS       DAORVN010000312         2416 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_43401_length_2416_cov_2.799238, whole genome shotgun sequence.
ACCESSION   DAORVN010000312 DAORVN010000000
VERSION     DAORVN010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2416
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43401_length_2416_cov_2.799238"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..628)
                     /locus_tag="PKV23_06920"
     CDS             complement(<1..628)
                     /locus_tag="PKV23_06920"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HQX85202.1"
                     /translation="MATEIKTPTFIHLRAHSAYSLAEGALHIKQMAVLAKEAGMPALA
                     MTDSGNLFGALEFSEVLADKGIQPIIGCSLRVETHAAAVEATTKPQPHSKKLPPLVLL
                     AKDETGYRNLMKLSSRAYLQTPENELPHATWEMLEELGGGLICLSGGPDGPIDEALVQ
                     GQEPLARQLAERLKRLFGDRFYIELQRHGTEAERAAEVGLVELAYELEV"
     gene            complement(668..1351)
                     /locus_tag="PKV23_06925"
     CDS             complement(668..1351)
                     /locus_tag="PKV23_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012112135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQX85203.1"
                     /translation="MTPALELRHVARSYAEGAGRLDIFKDLALEVYAGEIVALVGQSG
                     AGKSSLLHIAGLLEAPTAGEVFISGQNCTALDDAARTRIRRIGIGFVYQFHHLLPEFS
                     ALENVVMPQLIAGAKKPAAVTRAKDLLDRLGLAERFEHRPSEMSGGEQQRVAIARAIA
                     NRPLLLLADEPTGNLDPDTAARVHVEFLRLIKEEGLGALIATHNIPLASRMSRIVSLQ
                     SGILVDGLA"
     gene            complement(1351..>2416)
                     /locus_tag="PKV23_06930"
     CDS             complement(1351..>2416)
                     /locus_tag="PKV23_06930"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HQX85204.1"
                     /translation="SPIPDFKEIQARLEKVDGVKRVISLVEGQAMASSLKSSTGVLVR
                     GIAEADIEKLSSINNKDLQTSIAEPGVPDLTPSFAGFDKSEGIAVGERLAWRHQLKLG
                     SMLTIISPDGPDTVMGTAPRIRDFPVVAIFKIGMSEYDEGIAYLPLADAQEFFVSEEG
                     ATGLEVMVNSPDDIGALVPALRDAAGPELWMQTWKDRNQAFFNALAVERNVMFLVLTM
                     IILVAALNIISGLIMLVKDKGRDIAILRTMGATRGAVQRVFLITGAAIGVVGTFAGLI
                     LGILICLNVESIRQLVQWVSGVDPFNAEIYYLAQLPAVIDYSQTFWIVVMSLVLSLLA
                     TLYPSWRAAKLDPVEALRYE"
BASE COUNT          506 a          806 c          656 g          448 t
ORIGIN      
        1 gcacttccag ttcataggcc aattccacca agccgacctc agctgcgcgc tccgcctccg
       61 tgccgtggcg ttgcaattca atatagaagc ggtcaccaaa cagccttttg aggcgctccg
      121 ccaattgcct ggcaagcggc tcctgcccct gcacaagcgc ctcatcgata gggccatccg
      181 gcccaccgga taaacaaata aggccgccgc ccaactcctc aagcatttcc caggtcgcgt
      241 gcggcagctc gttttccggg gtctgcaaat aggcccggct cgagagcttc atcaggttgc
      301 ggtaacctgt ttcgtccttc gccaaaagca ccagaggcgg cagtttcttg gaatgaggct
      361 ggggcttcgt cgtcgcctca actgcagccg catgggtttc cacccgcaat gaacagccga
      421 tgatcggttg aatgccctta tcggccagca cttcggaaaa ttccagcgcc ccgaaaagat
      481 tgcccgagtc ggtcatggcc agcgccggca tcccggcttc cttggccagc accgccatct
      541 gcttgatgtg cagcgcccct tccgccagcg aataagcgga atgcgcccgc aaatgaatga
      601 atgtaggagt ttttatttcg gtggccatgc cggaagacta gcccagattc gcccgagccg
      661 tcaggcccta ggccagccca tccacaagaa tgccggactg caggctcacg atccggctca
      721 tccggcttgc caggggaatg ttatgggtgg caatcagcgc tcccagccct tcctccttga
      781 taagccgcag gaattccaca tgcactcgcg ccgccgtatc cggatcaaga ttgcccgtgg
      841 gttcgtcggc caaaagcaac aagggccggt tggcaatggc ccgggcaatc gccacccgct
      901 gctgctcgcc gcccgacatt tccgaaggcc ggtgttcaaa ccgttccgcc agccccagcc
      961 ggtccagaag gtccttcgcc cgcgtcaccg ccgcaggctt ctttgctccc gcgatcaatt
     1021 gcggcatcac cacattttcc agcgccgaga actccggcaa caggtgatgg aactgataga
     1081 caaagccaat gccgatgcgc cggatgcggg tgcgcgctgc atcatcgagt gctgtgcagt
     1141 tctgcccgga aatgaaaacc tcgcccgccg tcggcgcttc cagcagcccg gctatatgca
     1201 gcagtgagga tttgcccgcc cccgactggc ccaccagcgc aacgatctcg ccggcataaa
     1261 cctcaagcgc cagatccttg aaaatatcaa gccgccccgc cccttccgca taggagcggg
     1321 ccacatggcg aagctcaagg gcaggcgtca ttattcgtac ctcagggctt caaccggatc
     1381 gagcttcgct gcccgccatg aaggataaag cgtggcgagc agggagagga ccagcgacat
     1441 caccacgatc cagaaggtct ggctgtagtc gatgaccgcg ggaagctgcg ccaggtaata
     1501 gatttccgca ttgaacggat caacccccga cacccactgc accagttgcc ggatgctttc
     1561 cacattaagg cagataagaa tccccagaat gagcccggca aacgtcccta ccacaccgat
     1621 ggccgccccc gtgatcagaa acacccgttg caccgcgccc cgcgtcgccc ccatggtgcg
     1681 taaaatggca atgtcgcggc ccttgtcctt caccagcatg atgagtccgg aaatgatgtt
     1741 gagcgccgcc accaggatga tcatcgtcag caccaggaac atcacattgc gttccaccgc
     1801 cagcgcattg aagaaagcct ggttccggtc tttccaggtc tgcatccaaa gctccggccc
     1861 cgccgcatcg cgcagtgcgg gaacaagggc cccgatgtca tcaggggaat tcaccatcac
     1921 ctcaagcccc gtagcgccct cttcggagac gaaaaattcc tgcgcgtcgg ccagcggcag
     1981 ataggcgatg ccctcgtcat attccgacat gccgatcttg aagatggcga caaccggaaa
     2041 atcccggatg cgcggcgccg tgcccatcac cgtgtcgggg ccgtccggcg aaatgattgt
     2101 cagcatgctg cccagtttga gctggtgccg ccacgccagc cgctcgccga ctgcaatgcc
     2161 ttccgacttg tcgaatcccg cgaaggaagg agtcaggtcg ggaactcccg gctcggcaat
     2221 cgaggtttgc agatccttgt tgttgatgct ggagagcttc tcgatatcgg cttcggcaat
     2281 gccgcgcacc agaacgccgg tgctgctctt gagggaggag gccatcgcct gcccctcgac
     2341 cagcgagatc acccgcttca cgccgtcaac cttttcaagc cgcgcctgaa tctctttaaa
     2401 atccggaatg gggctc
//
LOCUS       DAORVN010000313         2413 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_43511_length_2413_cov_2.659457, whole genome shotgun sequence.
ACCESSION   DAORVN010000313 DAORVN010000000
VERSION     DAORVN010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2413
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43511_length_2413_cov_2.659457"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            141..1495
                     /locus_tag="PKV23_06935"
                     /pseudo
     CDS             141..1495
                     /locus_tag="PKV23_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598277.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
     gene            1576..>2413
                     /locus_tag="PKV23_06940"
     CDS             1576..>2413
                     /locus_tag="PKV23_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012110458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HQX85205.1"
                     /translation="MTTFRPKFSPSRRTLLKGTGAMFAGLTLLPRFSLAEEEKKLNLY
                     NWDTYIGETTLADFTAATGVEAKMDLFADNDELFAKLKGGNPGYDVIVPTNDMLERMI
                     KANMVMPLDHAKIPNMANIDKPFQDAAFDPGRKHSIPYMWGTLGVGYRKSKVEGGVVD
                     SWKILLDSDKYAGRLSLLGDAQNVIGVALKYLGHSFNSVDAAELKKAEDLLIAQKKYV
                     KVFADDNGQDLLASGEVDVWQEWNGDIIQVMAEDEDIAYAVPTEGSLVWQDTMAIPTG
                     APH"
BASE COUNT          531 a          722 c          670 g          490 t
ORIGIN      
        1 ggacccggaa ccggcacgac gaagtcatgg tcaattgcgc ccgcatcggg ttcaagccct
       61 agcacgcctc cgaattgcag ttctgataac gcattcaatt ttggagaaaa gccgtgtccg
      121 cacatcaaaa gaacgtcaat atcgctgaag tcgtccagca cgacatcgac catcattttc
      181 acccctttac cgaccacaag gccttccatg ccgccggcgg tccccgggtc atcacccatg
      241 ccgatggcgt ctatatctgg gatggcaagg gcaacaagat tctcgacggc ttttccggcc
      301 tgtggtgcgt gaatgtgggc tatggccgca aggagctggc cgaggtggcc taccgccaga
      361 tgctggatct gccctattac aacaccttct tcaaaaccac gacggtgccg acaactgagc
      421 tcgccgccaa gatctcctcc gttttgccca agcgcttcca gcatattttc ttcaccaatt
      481 ccggctcgga atccaacgac accatcgtgc gtttcgtgcg ccactactgg aagctgaagg
      541 gcaagcccta ccgccagcat ttcatcggcc gggtcagggg ctatcacggc tccaccatgg
      601 tcgccgccaa tctcggcgga atggccggaa tgcatgagca ggtgggaacc gaattcgcgg
      661 gtttccacca tgtccagcag ccctattggt atgatttcgg cggcgacaag acgcccgagg
      721 aattcggcct tgaggcggct gctgccgtgg aaaagaaaat cctcgaagtg ggccccgaaa
      781 atgtcgccgc ctttatcggc gaacccatcc agggcgcggc cggggttctc atcccgccga
      841 cgacttattg gcccgaggtg cagcgcatct gccggaaaca cggcgtcctg atcatttccg
      901 acgaagtgat ctgcggtttt ggccgcaccg gcaagtggtg gggcttccag tccctcggct
      961 tcgagccgga cattgtcacc atggccaagg cctgtcctcg ggctaccagc ccataggtgc
     1021 ggtggccttt gccgataccc tgattaagga ttttttcgct ctcggcggcg aattttacca
     1081 tggcatgacc tatgcgggcc atcctgtggc ggccgccgtc gccctgaaaa acatcgagat
     1141 catcgaggac gagaagctgg tggaaaaggt ggccgaattg gccccttact tcgccagggc
     1201 cctggccagc ctgaatgacc atcccatcgt gggcgaaacc cgcagccttg gcctgctcgg
     1261 cgccattgaa ctgtccaatg acaaggccaa acgcggcagg ttcaaggagt caggccgggt
     1321 cggcaccatc tgccgagacc actgcttcag gaacaatctg atcatgcggg cctgctggga
     1381 caccatggtc ctggcgcctc cgttcatcat ctccaaaaag gaaatagatg acatggttaa
     1441 actggcgcgc ctggcccttg atgcgaccca tgaggacgtt aaggctgaga tgtaattgaa
     1501 ttagaattta gttggaattt aggaagaaaa gggacaagct taacacaggc tcgcccgtac
     1561 tcgaaaggaa tgaccatgac cacatttcgt ccaaaatttt cacccagccg ccgcaccctc
     1621 ttgaagggca ccggcgccat gttcgccggc ctcacgctgc tgccgcgctt ctcgcttgcc
     1681 gaggaagaga agaagctcaa tctctataac tgggacacct atatcggtga aaccaccctt
     1741 gcggatttca ccgctgccac cggcgtcgag gccaagatgg atctgttcgc cgacaacgat
     1801 gagctctttg ccaagctcaa gggcggcaac cctggctacg atgtgatcgt tcccaccaac
     1861 gacatgctgg agcgcatgat caaggccaac atggttatgc cgctcgacca cgcgaagatt
     1921 ccgaacatgg ccaatatcga caagccattc caggacgccg ccttcgatcc gggccgcaag
     1981 cattcgatcc cctacatgtg gggcaccttg ggcgtcggct accgcaagtc aaaggtcgag
     2041 ggtggtgtgg ttgacagctg gaagattctg ctcgacagcg acaaatatgc gggccgtctt
     2101 tcccttctcg gcgacgcgca gaacgtgatc ggcgtggccc tcaagtacct cggccattcc
     2161 ttcaattcag tcgatgccgc ggagctcaag aaggctgagg atctgctgat tgcccagaaa
     2221 aaatatgtca aggtattcgc cgatgacaac ggccaggact tgctcgcttc gggcgaagtc
     2281 gatgtctggc aggaatggaa tggcgacatc atccaggtca tggccgagga cgaggatatt
     2341 gcctatgcgg tgccgacgga gggttctctt gtctggcagg atacaatggc aatacccacc
     2401 ggtgctcccc atc
//
LOCUS       DAORVN010000314         2405 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_43767_length_2405_cov_3.762128, whole genome shotgun sequence.
ACCESSION   DAORVN010000314 DAORVN010000000
VERSION     DAORVN010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2405)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2405
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43767_length_2405_cov_3.762128"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..743
                     /gene="rdgB"
                     /locus_tag="PKV23_06945"
     CDS             <1..743
                     /gene="rdgB"
                     /locus_tag="PKV23_06945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HQX85206.1"
                     /translation="KHFESQGIAIERIVDWPDGGRSIYVRDPAGNSVEFAESRIWHLP
                     ELKTLRNQKIVVATHNQGKLREINELLSPHGVTAVSAGSLGFDEPEETESTFTGNAKL
                     KAVYAAERSSLVALADDSGLCVDALNGDPGVYTANWAGPDRDWSMAMRLVEEKLQALG
                     ANTPEKRRANFNCTLCVAWPHGEVQFFEGRVYGHLTWPPRGTRGFGYDPMFVPDGETQ
                     TFGEMDPARKTLMSHRGRAFGRLVDALL"
     gene            730..1881
                     /gene="hemW"
                     /locus_tag="PKV23_06950"
     CDS             730..1881
                     /gene="hemW"
                     /locus_tag="PKV23_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019964187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HQX85207.1"
                     /translation="MPSSSDAFGIYVHWPFCKAKCPYCDFNSHVRHQEVDTMSFAKAL
                     TMELRWFADITPGRRVSSIFFGGGTPSLMPPAAVAEVIDQIAQLWPVADTVEITLEAN
                     PTSVEAENFRGYRAAGVNRVSVGVQALNEKDLKALGRQHTPDEALKAFRLAAKIFPRV
                     SFDMIYARPQQTAAQWKEELTRALGEQEGHMSLYQLTIEPGTAYFDLQARGSLVVPDE
                     DSATNLFEITQTLTEKHGLSAYEVSNHARVGQESRHNLLYWRYGEYVGVGPGAHARIA
                     EGENRRAIAMEKYPETWRKLVGEKGHGIVEDTTVLPVDQASEYLIMGLRVSDGIDLAR
                     YEKLRGTSMDPNKIAGLKSLGLVKREGHRLMATTQGRRLLNALITELAN"
     gene            complement(1862..>2405)
                     /locus_tag="PKV23_06955"
     CDS             complement(1862..>2405)
                     /locus_tag="PKV23_06955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HQX85208.1"
                     /translation="AAKSYAREAAAVTQPAASIAASLGQGSALLLPEGGQMLRTVSGA
                     LEGNGISPRSTKILGTGLWDDGLTRSTPIAQGGWYAGVAPQLVQAFEQKYAASYGVKP
                     PRIASLAYDAVAFAVNLQKTGDVSEVGIASPTGFQGSNGLFRFRENGLIERGLAILQM
                     GPGGPDVIQQAPTSFSSPAQ"
BASE COUNT          472 a          721 c          767 g          445 t
ORIGIN      
        1 aaaaacattt cgaatctcaa ggcatagcca tcgagcgcat cgtggattgg cccgacggcg
       61 gacgatcaat ctatgtgaga gatcccgccg gaaactcggt tgagtttgcg gaaagccgga
      121 tctggcattt gcctgagttg aagacgctca ggaatcaaaa aatcgtcgtg gccacccaca
      181 atcagggcaa gctgcgcgag atcaacgaat tgctgtcgcc ccatggggtg accgccgtgt
      241 ctgctggctc cctcggcttt gatgagccgg aagaaaccga atccactttc accggaaacg
      301 ccaaactgaa agccgtttac gcagcggagc gctcgagcct tgtggcgctg gccgatgatt
      361 ccggcctgtg tgttgacgcg ctcaatggtg atcccggggt ctatacggcg aactgggccg
      421 ggccggaccg ggactggtcc atggccatgc gcctggttga ggaaaaactg caggcgcttg
      481 gcgccaatac ccctgagaaa cgccgcgcca atttcaattg caccctgtgc gtggcctggc
      541 cccatggcga agtgcagttt ttcgagggcc gggtttacgg ccatctgacc tggccgccgc
      601 ggggcacgcg cggctttggc tatgacccga tgtttgtgcc ggatggcgaa acacaaactt
      661 ttggcgaaat ggatccggcc cggaaaaccc tgatgtcaca tcgcggccgg gcctttggaa
      721 ggctggtcga tgccctcctc tagcgacgcc ttcggcatct atgtgcattg gccgttctgc
      781 aaggccaagt gcccctattg cgatttcaac tcgcatgtgc gccaccagga agtggacacg
      841 atgtcgtttg cgaaagcgct gacgatggaa ttgcgctggt tcgccgacat cacgccgggc
      901 cgcagggtga gcagcatttt cttcggcggc ggcacgccgt ccctcatgcc gccggcggcg
      961 gtggccgaag tcattgacca gatcgcccag ctctggcccg ttgccgacac ggttgaaatc
     1021 acccttgagg ccaatcccac cagcgtcgag gccgaaaatt tccggggcta ccgcgcggcg
     1081 ggagtgaatc gcgtgtccgt tggggtgcag gcgctcaatg aaaaagacct gaaggcgctt
     1141 ggccgccagc atacgcccga cgaggcgctt aaagccttcc gtttggccgc caagattttc
     1201 ccgcgcgtct cctttgacat gatctatgcg cggccgcaac agacagcagc gcaatggaaa
     1261 gaggaactga cgcgggccct gggcgaacag gagggccaca tgtcgctcta ccagctcacc
     1321 atcgagcccg gcaccgccta tttcgacctg caggccaggg gttcgctcgt ggttcccgat
     1381 gaggacagcg ccacgaacct gttcgagatc acgcaaacac ttaccgagaa gcacgggctt
     1441 tcggcctatg aggtttcaaa ccatgcgcgc gtggggcagg agtcgcggca caacctgctg
     1501 tactggcgct acggcgaata tgtgggtgtc ggtcccggtg cgcatgcgcg cattgccgaa
     1561 ggcgagaacc gccgggccat tgccatggaa aaatatccgg agacctggcg caagctggtt
     1621 ggcgaaaaag gccatggcat tgtggaagat actaccgtgc tgccggtgga ccaggcgtcg
     1681 gaatatctta tcatggggct tcgcgtcagc gacgggattg acctggcgcg ttatgaaaaa
     1741 ctgcgtggga catccatgga ccccaacaaa atcgccggat tgaaatcgct gggcctggtc
     1801 aagcgcgagg gccatcgcct gatggccacg acgcagggcc gccgcctgct caatgcgctc
     1861 atcactgagc tggcgaactg aagctcgtcg gcgcctgctg gatcacatcg gggccgccgg
     1921 gacccatctg caatatggcg aggccgcgct cgatcaggcc gttctcgcgg aagcggaaca
     1981 ggccgttgga accctggaag cccgtggggc tggcaatgcc aacttcggac acgtcaccgg
     2041 ttttctgcag gttcacggca aaggccacag catcataggc gaggctcgca atgcgcggcg
     2101 gcttaacgcc atagcttgcc gcatatttct gttcgaaggc ttggaccagt tgcggggcca
     2161 cgcccgcata ccagccgccc tgggcaatgg gagtagagcg ggtaagcccg tcatcccaca
     2221 ggccggtgcc gagaatcttc gtgctgcgcg ggctgatgcc gtttccttcc aaggcgccgc
     2281 tgacggtgcg cagcatctgg ccgccttccg gcagaagcag ggccgagccc tggcccagcg
     2341 aggcggcgat gctggcggcg ggctgggtca cggcggcggc ttcgcgggca tagctttttg
     2401 ccgcc
//
LOCUS       DAORVN010000315         2404 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_43818_length_2404_cov_2.602810, whole genome shotgun sequence.
ACCESSION   DAORVN010000315 DAORVN010000000
VERSION     DAORVN010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2404
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43818_length_2404_cov_2.602810"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..715
                     /locus_tag="PKV23_06960"
     CDS             <1..715
                     /locus_tag="PKV23_06960"
                     /inference="COORDINATES: protein motif:HMM:NF040370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85209.1"
                     /translation="QIEFNEKTKTDFGNRADCTVWDEPFYAFSLLNHGNDHPMRAEII
                     AANESGWEALVAKCTGPSAKPVFYQKHMTHHMLPGFDRSWIHRLTNAFLIRAPERVLA
                     SYTKKWADVTLRDIGFVEQAEIFDAVAQKRGSAPPVIDADDVLKNPRGVLGALCKACE
                     IPFDEAMLKWPKGPKSFDGVWAPHWYNAVWESTGFAAPVEKTVELPLPLAKIADEAQG
                     PYLRLKAHSLVGVGGHQAG"
     gene            complement(683..1069)
                     /locus_tag="PKV23_06965"
     CDS             complement(683..1069)
                     /locus_tag="PKV23_06965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HQX85210.1"
                     /translation="MRSRETLLRLHRFRTDDRRRQAADIDGMIQDLTRKYDELDSHVR
                     FEESRNGVTDPANVNYSMAAKATRGRRDNLLKSISDLKDQKAAVLAQLADEEVELRKV
                     EMLMEKEGNASSSASGSAGLVAAHAH"
     gene            1467..2162
                     /locus_tag="PKV23_06970"
     CDS             1467..2162
                     /locus_tag="PKV23_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011581547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HQX85211.1"
                     /translation="MRVLLIEDDTATAQSIELMLKSEGFNVYTTDLGEEGVDLGKLYD
                     YDIILLDLNLPDMSGYEVLKSLRVSKVGTPILILSGLAGIEDKVKGLGFGADDYMTKP
                     FHKDELVARIHAVVRRSKGHAQSVILTGELLVNLDTKTVEVGGQRVHLTGKEYQMLEL
                     LSLRKGTTLTKEMFLNHLYGGMDEPELKIIDVFICKLRKKLAHACEGENYIETVWGRG
                     YVLRDPGEEQQAA"
BASE COUNT          556 a          659 c          675 g          514 t
ORIGIN      
        1 tcaaattgaa ttcaatgaaa aaacaaaaac tgattttggc aatcgcgccg attgcaccgt
       61 ttgggatgaa ccattctacg ccttctcgct gctcaatcac ggcaacgacc atcccatgcg
      121 tgccgaaatc attgccgcca atgaaagcgg ctgggaagcc cttgttgcga aatgcaccgg
      181 gccctcggcc aagcccgtat tctaccagaa acacatgacg caccacatgc tgccgggctt
      241 tgaccgcagt tggattcacc ggctgaccaa cgctttcctc atccgcgcgc cggagcgcgt
      301 gctcgccagc tatacaaaga aatgggcgga tgtgaccctc cgcgatatcg gctttgtcga
      361 gcaggcggaa atttttgatg cggtggcgca gaagcgcgga agcgccccac ccgtcatcga
      421 tgccgacgat gtcctgaaaa atccgcgcgg ggttctcggt gccctttgca aggcctgcga
      481 aattccgttt gacgaagcca tgttgaaatg gcccaaagga cccaagagct ttgacggcgt
      541 ctgggccccc cattggtaca atgccgtttg ggaatcaacg ggctttgccg cccccgtgga
      601 aaaaacagtt gaactgcctt tacctctggc gaagatcgcc gatgaggcgc aaggccccta
      661 cttgcgcctg aaggcccaca gcttagtggg cgtgggcggc caccaggccg gctgaaccgg
      721 atgcagacga tgatgcattg ccttcttttt ccatcagcat ctcgaccttg cgcaattcga
      781 cttcctcgtc ggccagctgc gccagaacag cggctttctg gtccttgaga tcggatatgg
      841 atttcaggag attgtcgcgg cgtccgcggg tggccttggc ggccatcgag taattgacgt
      901 tggccggatc ggtgaccccg ttgcggcttt cttcaaaccg gacatgggag tcgagctcgt
      961 cgtacttccg cgtgaggtcc tggatcatgc catcgatgtc ggctgcctgg cggcggcggt
     1021 catcggtgcg gaagcggtga agtcgcagaa gggtctcacg tgaacgcatg gaagtcctca
     1081 caaactcaaa aaacaaaccg ggcctcaaac cagcccctag gcacgattcg ccgaatcaac
     1141 taaccagaag attcgagtgc ttcaatcaac atcatgaacg ggtaagctta aggctgagta
     1201 aactgcgcgg gtgttgcaat taaaacgctg tccggtcgga ataatttggg tcaggatgct
     1261 acatgtggta tgctcggcaa gttcgaacac tagatatggc atatagtagg taaaagtgtt
     1321 ccgcctctag attaggtttt gttaaccttt ctaagcttat cttaacattc agagttaacg
     1381 gatagttgcg cgtgaagggc ccgttgcccg ttaggctgcg cgactgaaaa ggctccacca
     1441 gggagccgca taaaaggggt ttcgagatga gagtactgct gatcgaggac gataccgcaa
     1501 cggcccagag cattgagctg atgctgaagt ccgaaggctt caatgtttac accaccgatc
     1561 tcggcgagga aggcgtggac ctcggcaagc tctatgacta cgacatcata ctgctcgatc
     1621 tcaacctgcc cgacatgtcg ggctatgaag ttctgaaatc gctccgcgtt tcaaaagtcg
     1681 gaacacccat tctcattctc tccggtcttg ccggcatcga agacaaggtc aagggcctgg
     1741 gcttcggcgc cgatgactac atgaccaagc ctttccacaa agacgagctt gtggcccgca
     1801 tccacgcggt ggtgcgccgc tccaagggtc atgcccaatc ggttatcctc accggcgagt
     1861 tgctggtgaa ccttgatacc aaaacagtcg aagtgggtgg ccagcgcgtg cacctcaccg
     1921 gcaaggaata tcagatgctc gagctgcttt cgctgcgcaa gggcacgacg ctcaccaagg
     1981 aaatgttcct caaccacctt tacggcggca tggacgaacc cgaactcaag atcatcgacg
     2041 tgttcatctg caagctgcgc aagaaactgg cgcacgcctg cgaaggtgaa aactacatcg
     2101 aaaccgtctg gggccgcggc tatgtgctgc gcgatccggg cgaagaacag caagctgcct
     2161 aagctagctg acatccacgg ttaccctaac ggctcgccag caatggcggg ccattttggt
     2221 tttgtgttgg gccagctgtg tttgaacggt tagaaacctt atcggttcaa tcgcacaggg
     2281 gtaaatagtc atgatcgatt tccagaacgg ttcggtgttt aagcttaagc agaacagcga
     2341 atacgcggcc aatgttggcg cgctgctgct ggagggggaa agcgtgctgg gtgccttcaa
     2401 ggca
//
LOCUS       DAORVN010000316         2402 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_43879_length_2402_cov_2.845334, whole genome shotgun sequence.
ACCESSION   DAORVN010000316 DAORVN010000000
VERSION     DAORVN010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2402
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43879_length_2402_cov_2.845334"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="PKV23_06975"
     CDS             complement(<1..314)
                     /locus_tag="PKV23_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010915593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP cone domain-containing protein"
                     /protein_id="HQX85212.1"
                     /translation="MRCPFCSNEETQVKDSRATEDGAAIRRRRYCPDCAGRFTTFERV
                     QLRELMVIKKTGRRVPFDRDKLMRSVQIALRKRAVEPERIERMVSGILRRLESLGESE
                     IKS"
     gene            complement(327..1667)
                     /gene="glyA"
                     /locus_tag="PKV23_06980"
     CDS             complement(327..1667)
                     /gene="glyA"
                     /locus_tag="PKV23_06980"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008836718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HQX85213.1"
                     /translation="MAKKASKKTVKKPAAKAKAKKAPLFPGFFKEKLAKTDPKVAQAI
                     TRELYRQQNEIELIASENIVSRAVLEAQGSIMTNKYAEGYPGRRYYGGCQYVDVSETL
                     AIERACKLFDCSFANVQPHSGAQANQGVFFALLQPGDTYMGLDLACGGHLTHGSPVNQ
                     SGKWFHVAPYKVREDNHQIDYDALAAQALQVKPKLIIAGGSGYPRFLDFARFRQIADS
                     VGAYLMVDMAHIAGLVAGGVHPNPLEYAHVVTTTTHKTLRGPRGGMILTRDEAIGKKI
                     NSSVFPGLQGGPLEHVIAAKAVAFGEALRPAFKKYAAQVVANARALADAMVAGGCDLV
                     SGGTDTHLMLVDLRKKGLTGKASEAALGRAYITCNKNGIPFDPAPFTITSGIRLGTPA
                     GTTRGFGVAEFREVGALIIEVLDGLAKHGEAGNAKVEAAVKRKVLALTKRFPIY"
     gene            complement(1795..>2402)
                     /locus_tag="PKV23_06985"
     CDS             complement(1795..>2402)
                     /locus_tag="PKV23_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012111828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HQX85214.1"
                     /translation="ARRYGALTYLDEVHAVGMYGPRGGGIAERDGLLAEIDIIEGTLA
                     KAFGLMGGYIAANATIIDAVRSFAPGFIFTTSIAPAIAAGATAAIRHLKASSLERQLQ
                     SRHAKLLTASLKARGLPVTETESHIVPLIVGDPVKCKALTDRLLSDFSIYVQPINYPT
                     VPKGTERLRLTPGPLHGELEISRLVAALDQLWTEMLLQRAA"
BASE COUNT          455 a          678 c          782 g          487 t
ORIGIN      
        1 gacttgatct cgctttcgcc aaggctttcc agcctgcgga ggatgccgga caccatgcgt
       61 tcaatccgct cgggttccac cgcgcgcttg cggagcgcta tctggaccga gcgcatgagc
      121 ttgtcgcggt cgaagggaac acgcctgccg gttttcttga tcaccatgag ttcgcgcagc
      181 tggacacgct caaaagtggt aaagcgtccg gcgcaatccg gacaataacg ccggcggcgg
      241 atcgcggcgc catcttcggt ggcgcgcgaa tccttcacct gggtttcttc gttactgcag
      301 aatgggcagc gcatggcttc ctttgcttag tagatgggga agcgcttggt gagagccaag
      361 accttgcgct tgaccgccgc ttccacctta gcattgcccg cttcgccgtg cttcgcaagg
      421 ccatcgagga cctcgatgat cagggctccc acttccctga actccgccac gccaaagccg
      481 cgggtggtcc ccgctggcgt tcccaggcgg atgccggagg tgatggtgaa gggtgcgggg
      541 tcaaacggaa tgccgttctt gttgcaggtg atgtaagccc gtcccagggc ggcttcggag
      601 gccttgccag tcaaaccctt cttgcggagg tccaccagca tcaaatgggt gtccgtgccc
      661 cctgagacca gatcacagcc gcctgcaacc atggcatcgg ccagggccct ggcattggcc
      721 acgacctggg ccgcatattt tttgaaagcg ggtcgaaggg cttcaccaaa ggccacggcc
      781 ttggcggcaa tcacatgctc caacggccca ccctggaggc cggggaatac ggaagaattg
      841 atcttcttgc caatggcttc atcacgcgtg aggatcatgc cgccgcgggg accgcgcaag
      901 gtcttgtggg tggtggtggt cacgacatgg gcgtattcaa gcggattggg gtgcacacca
      961 ccggccacaa ggcctgcgat atgggccatg tccaccatca gataggcgcc aacactgtcg
     1021 gcgatctggc ggaagcgggc aaaatcgagg aagcgcggat agcccgaacc accggcgatg
     1081 atgagtttcg gtttgacctg gagagcctga gcggcgagag cgtcgtaatc gatctggtga
     1141 ttgtcctcgc gcactttgta gggcgccaca tggaaccact tcccggactg gttcacgggt
     1201 gagccgtggg tcagatgccc gccacaggcg agatcgaggc ccatgtaggt gtcgcccggc
     1261 tggagcagcg cgaagaatac gccctggttg gcctgggcgc cggaatgggg ctggacgttg
     1321 gcaaaggaac agtcaaacag tttgcaggcg cgctcaatgg cgagggtttc ggacacgtcc
     1381 acatactggc agccgccgta atagcgcctg ccgggatagc cttcggcgta tttgttggtc
     1441 atgatcgagc cctgcgcttc cagcacggcg cgcgagacaa tgttttccga ggcgatcagt
     1501 tcaatttcgt tctgctggcg gtagagttcg cgggtaatcg cctgggcgac ctttggatcg
     1561 gttttagcta gtttttcctt gaagaagccc ggaaacaagg gagccttttt agccttggct
     1621 ttggcggccg gtttcttgac ggttttcttg cttgccttct tggccatgat ggtcccctac
     1681 cctgattccg cgtttcggct gcaaaatagc gaaaggcggg ttggggtcca gtaatatatg
     1741 gccgcagcga aattggcgtg ggagaagcgg cgtgaacaga caaaatagcg gcagtcaggc
     1801 ggcgcgctgc agcagcatct cggtccagag ctggtcaagg gccgccacca gcctggagat
     1861 ttcgagttca ccgtggaggg gccctggggt gaggcgcagg cgctcggtgc cctttggtac
     1921 cgtcggataa ttgatgggct ggacatagat ggaaaaatcg ctgagcaggc ggtcggtcag
     1981 ggccttgcac ttgaccgggt cgcctacaat cagggggacg atgtggcttt cggtttcagt
     2041 caccggaagc cccctcgcct tcaacgaggc cgtgagcagt ttcgcatgcc ggctctgcag
     2101 ttgccgctcg aggctgcttg ccttgaggtg gcggatcgcg gccgtggccc cggcggcgat
     2161 ggccggggca attgaggtgg tgaagataaa gcccggggca aaggacctga cggcatcaat
     2221 gatcgtggcg ttggcggcga tgtaaccacc catcaggcca aaagccttgg cgagcgtgcc
     2281 ttcgatgata tcgatttcag ccaataagcc atcgcgctcg gcaataccgc cgccgcgggg
     2341 gccatacatg ccaacggcgt ggacttcatc cagataagtg agagcgccat atcgcctggc
     2401 ga
//
LOCUS       DAORVN010000317         2400 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_43933_length_2400_cov_3.625586, whole genome shotgun sequence.
ACCESSION   DAORVN010000317 DAORVN010000000
VERSION     DAORVN010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2400)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43933_length_2400_cov_3.625586"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            147..716
                     /locus_tag="PKV23_06990"
     CDS             147..716
                     /locus_tag="PKV23_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HQX85215.1"
                     /translation="MGGILGYARVSTGDQDVAGQTMRLEKAGAIKVFTDVMSGKSMDR
                     PGLADLLAYARKGDTLAVVRLDRLGRSLAELLVTVEKLHAQGVALLSLEEKIDTSSAA
                     GELIFHVFGAIAHFERRLISERTKDGIAAARARGKLPGRQPLDMSKVHAAIKLVEASI
                     SPTEAARQLGIGRSTIYREMRRLGVERPA"
     gene            717..2090
                     /locus_tag="PKV23_06995"
     CDS             717..2090
                     /locus_tag="PKV23_06995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85216.1"
                     /translation="MSRSKSVHDLSGLMKYAERAPWDEAMVEMLSAHLGPACEATGLE
                     PDAIFEIIGDHWQGPLWGCSFEDLLTQELEPDGGNLIDDYLKRRGWNEKAPNKAYMHA
                     LRDTVMSLYEVSEVVPGQSMTLRDLLRDVAPVRVREHGATQTLVNWDKIAARIVDVNG
                     RHGISGALLPFSADGAVRVIDAFSRLVDDAQGTVGLDPLDKDALLRASGPMFTNMWLL
                     DCLGKAIPGSSPDMVNADGDDLVFHRIVFPLAKGVIGKGVVRRLNAAQWLEPASDTFW
                     NWLAPVTKKRTPGTTKGGQAFVTTMEDGTPVFANVELAGRKLIVEVNSAARAEQAIAQ
                     MSEWLGDCVSTPMTEIRTLAQFMADDAARAPQEEPLDIPPNEMERIVHQMLTREYTKT
                     LDEAVPALGHKTPRALARTKAGRPKVADWLKYIENGAAKSGPGDPMATYDFTWMWDQL
                     GLIDLRR"
     gene            2161..2400
                     /locus_tag="PKV23_07000"
                     /pseudo
     CDS             2161..2400
                     /locus_tag="PKV23_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003501156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
BASE COUNT          530 a          673 c          731 g          466 t
ORIGIN      
        1 taggaaatat aatggtcagg acagcgaaat cagcgcctta cttgggaaat gtggggtaaa
       61 gagacatagt atgtggacgg atatgtaggg gcgaaacgct tgcccttcac agcgtccaca
      121 gaacggccgt ttgggagaca cagggcatgg gcggtattct gggctatgct cgtgtgagca
      181 ccggcgacca ggacgtggca gggcagacca tgcgtctgga gaaggctggc gccattaaag
      241 tgttcaccga cgttatgtcg ggcaagagca tggatcgtcc cggtttggcc gacctgctcg
      301 cctacgcccg taagggtgac acattagccg tggttcgcct cgatcggctc gggcgatcgc
      361 tggccgaatt acttgtcacg gttgagaagc tgcacgccca aggcgtggcg ctgctaagcc
      421 ttgaagagaa aatcgacact tcatcggccg ccggcgaact gatcttccat gtgttcgggg
      481 ccatcgccca ttttgaacga cggctgattt ccgaacgcac caaggatggc atcgccgccg
      541 cccgtgccag gggcaagtta cctggccgtc agccgctcga catgagcaag gtccatgccg
      601 ccattaaact ggtcgaagcg agtatctcgc caacagaagc ggcacggcaa ctcggcatcg
      661 gccgatcaac catatatcga gaaatgcgcc gccttggcgt ggaaaggccc gcctgaatgt
      721 ccagatcaaa atcggttcac gatctttcgg gcctgatgaa atatgcggaa cgggcgccat
      781 gggatgaagc gatggttgaa atgctgtccg cgcatcttgg cccagcgtgc gaagcaaccg
      841 gactcgagcc agatgccata ttcgaaatca tcggtgatca ctggcaaggg ccgctatggg
      901 gctgttcctt tgaagatctg ctgacgcagg aactggaacc tgacggtggc aatctgatcg
      961 atgactatct caagcgccgg ggctggaacg aaaaggcgcc gaacaaagcc tatatgcacg
     1021 cactacgcga tacggtcatg tcgctctatg aagtcagcga agtggttccc ggccaatcga
     1081 tgaccttgcg cgatctgctg cgcgacgttg ccccggtgag ggtgcgggaa cacggtgcga
     1141 cccagaccct cgtcaactgg gacaaaatcg ccgcaagaat tgttgacgtg aacggtcggc
     1201 acggcatttc cggggcactt ctgccgttca gcgcagatgg cgccgtgcgc gtaatcgacg
     1261 cattttcccg acttgtggac gatgcacagg gcactgtcgg attggatccc ctagacaagg
     1321 acgcgctctt gcgggcatcg gggccgatgt tcacgaacat gtggctgctc gactgtctgg
     1381 gtaaggccat tcccggcagc tcgccggata tggtcaacgc cgacggtgat gaccttgtgt
     1441 tccaccgtat cgtttttccg ctggcaaagg gcgtgatcgg caaaggcgtg gttcgcaggc
     1501 tgaacgcggc tcaatggctg gaacctgcga gcgacacgtt ctggaactgg ctggcgccgg
     1561 tgacgaagaa gaggacacca ggaacgacca aaggcgggca ggcttttgtg acaaccatgg
     1621 aggacggcac acccgttttt gccaatgtcg agttggctgg acgcaaactg atcgttgagg
     1681 tcaacagcgc cgcccgcgct gaacaggcga ttgcgcagat gagcgaatgg cttggtgatt
     1741 gcgtgagcac acctatgacc gaaatccgga ctctggccca gttcatggcc gatgatgccg
     1801 cccgcgctcc gcaggaagag ccgctcgata tcccgccgaa cgaaatggaa cgcatcgttc
     1861 atcagatgct gacacgcgaa tataccaaaa cgcttgatga agcggtgccg gccctcgggc
     1921 acaagacgcc acgcgctctg gcccgcacga aggcgggccg accgaaagtg gccgactggc
     1981 tcaaatatat agagaatggc gcggcaaaat ccgggcctgg cgatccgatg gctacctatg
     2041 attttacgtg gatgtgggat caactggggc tgatcgacct ccggcgttga ccgccctcat
     2101 accaccgttc tggcatcgtt ctcccttagc gttgtctggc gtaccatcca cgctatgccc
     2161 tcatcaggcc tgaacctcgt atcggcggca atcatcctat ggaataccgt ctatcttggc
     2221 agggctgccg accatctccg ccaaaggggt cgcgccatag acgatacgct gctcagccac
     2281 gccgcgccgc taggctggga gcacatcagt ctgacgggtg attatctatg ggctgacatg
     2341 aaattgccac tggacgggtt tcgaccatta cgcacgcaaa accgccaatc agaggcttag
//
LOCUS       DAORVN010000318         2397 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_44032_length_2397_cov_3.771563, whole genome shotgun sequence.
ACCESSION   DAORVN010000318 DAORVN010000000
VERSION     DAORVN010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2397
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44032_length_2397_cov_3.771563"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1854)
                     /locus_tag="PKV23_07005"
     CDS             complement(<1..1854)
                     /locus_tag="PKV23_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012563954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HQX85217.1"
                     /translation="MKLGIAGQLTRYFIASPLTPLFLLAAFMVGLVALVTLPREEEPQ
                     ISVPMVDIHVRADGLKAEDAVKLVTEPLETIIKSIDGVEHVYSQTKDDGAMVTARFFV
                     GTNSDAAILRVHEKIRANMDRIPVGIPEPLIVGRGIDDVAIVVLTLSPRPEAAARWTA
                     NAVSRVARELRVELTKLDDIGLTYLSGDQPEEIRIEPDPEKLALYGVTLQQLSAKVSG
                     ANRAFEAGQVRDAGRQISVMAGQTLQSPTEIGNILITARDGRPVYVRDVAAVLLGADS
                     NEWRVTNVSKSDGGLLRVPAVSLAIAKRAGTNAVVISENIIARLETLKGEVIPEDISV
                     EVTRNYGETANEKANELLFHLGLATISIVILVAVSIGLREALVVAVVIPTTILLTLFA
                     ARVMGYTLNRVSLFALIFSIGILVDDAIVVIENIARHWAMNDGRSRTQAAIDAVAEVG
                     NPTIVATLTVVGALLPMLFVSGLMGPYMSPIPANASAAMIFSFFVAVIVTPWLMIKMG
                     KADGGHGHGASHGGRLGSMYVAVARPILRNRARAWTFLLAVGVVTLASLVLFYSKSVT
                     VKLLPFDNKSDLQIVIDLPEGSSVEDTDRVLQQAANRAATIAEVHSLQTYAG"
     gene            complement(1851..>2397)
                     /locus_tag="PKV23_07010"
     CDS             complement(1851..>2397)
                     /locus_tag="PKV23_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011581618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HQX85218.1"
                     /translation="GAEGDVLAPASGRVLSVPVTGGSVVLPGETIARIAGGGYFLRLS
                     LPERHAAQINQGDDVFVGSRGVGSATVTFEKARKGKLVKVYPEIEGGRVLADVEVDGL
                     GDFFAGERTLVWIPVDQRTAISVPPAAISTRYGIDYVSLVSAAGPMDVAVVTGESFET
                     GNGARVEILSGLRDGDRVVTP"
BASE COUNT          470 a          767 c          738 g          422 t
ORIGIN      
        1 acccgcatag gtttgcaggg aatggacctc ggcaatggtc gcggcccggt tggctgcctg
       61 ttgcagaaca cggtccgtgt cttcaaccga agatccttcc ggaaggtcga tcacgatctg
      121 caggtccgac ttgttgtcaa agggcagcag cttgaccgtg acactcttgc tgtagaacaa
      181 cacaagcgag gccagcgtca ccacacccac ggcaagaagg aaagtccagg cgcgggcgcg
      241 attcctgaga attggccgtg ccacggcaac atacatgctt cccagccggc ctccgtggct
      301 tgccccgtgg ccgtgcccgc catcggcctt tcccatcttg atcatcagcc atggcgtgac
      361 aatgaccgcc acgaagaagg agaagatcat ggcggccgag gcgttggccg gtatggggct
      421 catgtagggg cccatgaggc ccgagacgaa cagcatgggg agcagggcgc ccaccaccgt
      481 gagggtggcc acgatagtcg gattgccaac ttcggccacc gcatcaattg cggcctgggt
      541 gcgcgaccgg ccatcattca tggcccagtg gcgggcgatg ttctcaatca ccacgatggc
      601 gtcatcaacc agaatgccga tggaaaagat cagggcgaag aggctgacgc ggttcagcgt
      661 atagcccatc acacgggcgg cgaacaaagt cagcaggatg gtggtgggga tcaccacggc
      721 aaccaccagc gcttcgcgca ggccaatgga aaccgccacg agaatcacga ttgaaatggt
      781 ggccaggccc aggtggaaca gcagttcatt ggctttttcg ttggcagttt cgccgtagtt
      841 gcgcgtgact tcgacgctga tgtcctccgg aataacttcg cccttgagcg tctcgagccg
      901 cgcgatgatg ttttccgaaa tgacaacggc attggtgccg gcgcgcttgg caatggccag
      961 ggagaccgcc gggacacgca gcagcccgcc atcggacttg ctgacattgg tcacccgcca
     1021 ctcattgctg tccgcaccga gaaggaccgc cgccacatcg cggacataga ccgggcggcc
     1081 gtcgcgcgcg gtgatcagga tattgccgat ttccgtgggg ctctgcaggg tctggcccgc
     1141 catgacggag atctggcgac ccgcatcgcg gacctggccc gcctcgaacg cccggttggc
     1201 gccgctcacc ttggccgaca gctgttgaag cgtcacccca tagagggcaa gtttctcagg
     1261 atcgggttcg atcctgattt cctccggctg gtcgcccgag agataggtca gcccgatgtc
     1321 gtcaagcttg gtaagctcca cccgcagttc acgcgccacc cggctcacgg cgttggcggt
     1381 ccagcgcgcg gcggcttccg gcctgggcga cagcgtcaac acgacaatgg ccacatcgtc
     1441 aatgccgcgg cccacgatga gcggctcggg gatgcctacc ggaatacggt ccatgttggc
     1501 acggattttt tcatggacac gcaggatcgc ggcatccgaa ttggtgccga caaaaaagcg
     1561 cgccgtaacc atggcgccgt catccttggt ctgcgaatag acatgctcga cgccatcgat
     1621 gctcttgatg atcgtctcaa gcggctcggt caccagcttc accgcatctt cggccttcag
     1681 gccgtcggcg cgcacatgga tatccaccat cggcacggat atctgcggtt cctcttcgcg
     1741 cggcaaggtg accagtgcca cgaggcccac catgaaagcc gcaagcagaa acagcggcgt
     1801 cagcggcgag gcaatgaaat accgggtgag ttggccggcg attcccagct tcacggcgtt
     1861 accaccctgt ctccgtcgcg caacccggag aggatttcga cgcgggcccc atttccggtt
     1921 tcgaaacttt cgcccgtgac caccgccaca tccatggggc cggcggcgct gacaagactc
     1981 acatagtcaa tgccatagcg ggtgctgatg gcggccggcg gaacactgat ggcagtgcgc
     2041 tggtccacgg gtatccatac cagggtgcgt tccccggcaa agaaatcacc caggccgtcc
     2101 acctcgacat cggccagaac gcggccacct tcgatctcgg gatagacttt taccagcttg
     2161 cccttgcggg ccttttcgaa cgtcacggtg gcgctgccca cgccgcggct gccgacgaaa
     2221 acgtcgtcgc cctggttgat ctgcgccgca tggcgttcgg gcagtgacag cctgaggaaa
     2281 tagccgccgc cggctatccg ggcgatggtt tcacccggca ggacgacgga gcccccggtc
     2341 accggcacgg agagcacgcg gccggaggcc ggcgccagga catctccctc cgctccc
//
LOCUS       DAORVN010000319         2397 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_44034_length_2397_cov_3.632792, whole genome shotgun sequence.
ACCESSION   DAORVN010000319 DAORVN010000000
VERSION     DAORVN010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2397
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44034_length_2397_cov_3.632792"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1428
                     /locus_tag="PKV23_07015"
     CDS             <1..1428
                     /locus_tag="PKV23_07015"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HQX85219.1"
                     /translation="RAGVPMSNPKPGWFEQDVGDWTNGAAKALKQLSKKIDMARVGAI
                     AISNQRESFAQFDSANRPLRPGTLWLDERAHAEAKELAQLIGAEELHRISGKPSDVTP
                     CISRCLWLAKSMPGLWKKTAMTAEVHGILVHFLTGQWHTSTASADPMGLIDMEQYDWS
                     DELLAAARLSRSQLPKLFRPGEVMGEVTPAAARLTGLKAGTLVVAGGGDGQCAGTGAN
                     TFVAGRAYLNLGTAVVSGSFGKAYAHNPVFRTMSAVAEEGYSYETAIRTGTFLVNWMV
                     ERLFNVKASKNPGIFAALEREAKASPPGSRGVMLVPYWLGCMTPYWSPKARGVIAGLS
                     GNHSRGDIYRAVLEGIALEQVMMTDGVAAATQAIDHIVVMGGGAQSDLWCQIVADAGS
                     RDVRRLETVEASSLGAAMAAAKGAGWFKSIPAASESMSGKPSRTFRPRARENKAYREL
                     MAVYRDLWPTLSQWNARRQAISGDT"
     gene            1428..>2397
                     /locus_tag="PKV23_07020"
     CDS             1428..>2397
                     /locus_tag="PKV23_07020"
                     /inference="COORDINATES: protein motif:HMM:NF025065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HQX85220.1"
                     /translation="MTDWNALIRDVVKGDWPDPETGKPANVPFETIRIEETLEGGAAD
                     LVAPLRIGKRIAVVSDVNTHEALGRRVAKELRGLGSIDEVVMPGDTHCDEPTVARVQE
                     LTRHADGIVVVGSGSLSDSCKFATFKDGRKYACFGTANTMNGYAASTASVTLNNGYKT
                     SLPAHAPRGIFIDLKVCADAPTRLSAAGLGDSLCRPTAQIDWWASHRLFGTFYSATPY
                     ALMAEDEPKMIATAGDLAKHDVAANGHLQRVLMLCGFGVCFTGVSHHGSMGEHQVSHW
                     IDMFAGKDHPGTKHGQQVGVASLAIARLQNEILRMDKPPKIRATHIV"
BASE COUNT          479 a          730 c          746 g          442 t
ORIGIN      
        1 cgggccggcg tgcccatgag taatccgaag cccggctggt ttgagcagga tgtgggcgac
       61 tggacgaacg gcgcggcaaa ggcactcaag caactctcga agaaaattga catggcgcgg
      121 gtgggcgcaa tcgccatttc caaccagcgt gaatccttcg cgcaattcga ttctgccaac
      181 agacccctgc gccccggcac cttgtggctg gatgagcggg cccatgccga agccaaagaa
      241 ctggcgcagt tgatcggagc cgaagaactg caccgcattt ccggcaagcc cagcgatgtc
      301 acgccctgca tctcgcgctg cctgtggctg gccaaatcca tgcccggcct ctggaagaag
      361 acggccatga cggctgaagt gcatggaata ctggtgcatt ttctcaccgg ccagtggcac
      421 acatcaacgg cctcggccga tccgatgggg ctgatcgaca tggagcaata tgactggtcc
      481 gatgaacttc tcgccgcggc ccggttgagc cggtcgcaat tgcccaagct gttccgtccc
      541 ggagaggtca tgggcgaagt gaccccagcc gcagcccggc tcacgggcct gaaagcggga
      601 accttggtcg tggccggcgg cggagatggg caatgcgccg gcacgggagc caataccttt
      661 gttgccggcc gtgcctatct caatctcggc acagcggtcg tatcaggcag tttcggcaag
      721 gcctatgccc acaaccccgt gttcaggacc atgtccgccg tagcagaaga gggttattcc
      781 tacgaaaccg ccattcgcac cggaacattc ctggtgaact ggatggtcga gcgactattc
      841 aatgtgaagg ccagcaagaa cccggggata ttcgcggccc ttgagcggga ggcaaaggca
      901 tcgcccccgg gcagccgcgg cgtgatgctg gtgccctact ggcttggctg catgactccc
      961 tattggagtc cgaaagccag gggcgtcatt gccggactat cgggcaatca ctcacgcggc
     1021 gatatttacc gcgccgtcct cgagggcatt gcccttgagc aggtgatgat gacggatggt
     1081 gttgcagctg ccacgcaggc cattgaccat attgttgtca tgggcggcgg ggcgcagtcg
     1141 gacttatggt gccagatcgt ggccgatgcg gggagccgtg acgtgaggcg gctggaaacg
     1201 gttgaagcct cttcgcttgg agctgccatg gccgccgcca agggcgccgg atggttcaag
     1261 agcattcctg ccgcttccga aagcatgtcc ggcaagcctt ccaggacatt ccgcccgcgg
     1321 gccagggaaa acaaggctta ccgcgaactg atggcagtat accgcgactt gtggccaacg
     1381 ctttcgcaat ggaatgcgcg gcggcaagcc atttctggag acacctgatg acggactgga
     1441 acgcactcat acgcgatgtc gtgaagggtg actggccgga tccggaaacc ggaaagcctg
     1501 ccaatgttcc ctttgaaacc atccgcatcg aggaaaccct cgaggggggt gcagccgatc
     1561 tcgtggcgcc attgcgcata ggcaagcgca ttgccgtggt cagcgacgtc aacacccatg
     1621 aagccttggg ccggcgtgtc gccaaggagc tgaggggcct cggcagcatc gatgaagtgg
     1681 tcatgcccgg tgacacgcac tgcgatgagc cgacggttgc acgggtgcag gaacttacgc
     1741 gccatgccga tggcatcgtc gtcgtgggtt cgggttcgct ctcggacagc tgcaagttcg
     1801 cgaccttcaa ggacggccgc aaatatgcct gcttcggcac ggccaacacg atgaacggct
     1861 atgcggcatc caccgcttcg gtgaccctga acaatggcta caagacttcc ctgcccgccc
     1921 acgctccccg cggcatcttc attgacctca aggtgtgcgc ggatgcgcct acccggcttt
     1981 ccgccgcagg ccttggtgac agtctttgcc ggccaaccgc gcagatcgac tggtgggcct
     2041 cgcatcgcct gttcggcacc ttctattccg ccacgcccta tgcgctgatg gcggaagatg
     2101 agcccaaaat gattgccacg gcgggtgacc ttgcaaagca cgacgttgcc gccaatggcc
     2161 atctgcaacg cgtgctgatg ctgtgcggct tcggggtttg cttcaccggc gtttcgcacc
     2221 atggctcgat gggcgaacac caggtatccc actggatcga catgtttgcc ggcaaagacc
     2281 atccgggcac caagcatggc cagcaagtcg gcgtggcttc gcttgccatt gcacgcctgc
     2341 aaaatgaaat cctgagaatg gacaagccgc cgaagatccg cgccacccac attgtgg
//
LOCUS       DAORVN010000320         2396 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_44078_length_2396_cov_3.298163, whole genome shotgun sequence.
ACCESSION   DAORVN010000320 DAORVN010000000
VERSION     DAORVN010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2396
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44078_length_2396_cov_3.298163"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..716
                     /locus_tag="PKV23_07025"
     CDS             <1..716
                     /locus_tag="PKV23_07025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85221.1"
                     /translation="RDEPGRAIVAELETIVRDMGASKVKARALISIGLSYLNQGEELL
                     GLAKCEAGLAMHRATGNTRSGLRSGIFVAEVLHRWGNNAGAIALGMDLLRELRELGGY
                     KLERGYQYCNLAAYHLADGAADQALEALLEAIESVPRDDTFWHWAMLQNAAGIEAAAG
                     DPRRAARLMGFVDRRYGDFPDGRQHTEQRQRARIMEQLAAAIPAAELASLMKEGETIS
                     AFEADYFANFPVLELDGAA"
     gene            758..1885
                     /locus_tag="PKV23_07030"
     CDS             758..1885
                     /locus_tag="PKV23_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008193732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQX85222.1"
                     /translation="MQDGSDISHQPGRQRWGTRGTAGALFAGRLTFENVSCSIGSTEI
                     LKDLSFELQAGEIACLLGQSGCGKTTLLRIAAGIQRPTAGRVLLDGEEVDGPTRYVPP
                     ERRNVGLVFQDFALFPHLTVIENVAFGLSALSRGEAAKVAEHALARVGLASFRDSYPS
                     SLSGGEQQRVALARAIVPRPQVMLMDEPFSGLDQRLRETVRGETLALLQETRATCVLV
                     THDPVEAMDFADRILLMRAGRLIQTGSSVDLFNRPADLQVARFFSDVNELGAIVKNGN
                     VETPLGTFSAAGFDQGTEVVVIVRPQALSLAQAGAGTEGYVLEARFLGDKTRLQVQFN
                     GVDEPLAVLLSGLSPQRGETAHFRVDHNHVLIFTKPAPDSI"
     gene            1972..2202
                     /locus_tag="PKV23_07035"
     CDS             1972..2202
                     /locus_tag="PKV23_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019858561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HQX85223.1"
                     /translation="MGFGSWWHWVLLLIVVLVVFGGKGKVSDLMGDVAKGIKSFKKGL
                     ADDPAEAIAEEVMSLEKTAEEPVKSPAKKVKS"
BASE COUNT          429 a          661 c          818 g          488 t
ORIGIN      
        1 tgcgcgatga accgggccgt gccatcgtgg ccgaactgga aaccatagtt cgtgacatgg
       61 gagcttcaaa ggtcaaggcg cgagccctga tttcaatcgg tttgagttat ttgaaccagg
      121 gcgaggaatt gctgggcttg gccaaatgcg aggcgggcct ggccatgcac cgggccaccg
      181 gcaacacccg cagcggcttg cgctcgggaa tctttgttgc cgaagtgctg caccgctggg
      241 gaaacaacgc aggcgccata gcccttggca tggacttgct gcgcgagttg cgcgaactcg
      301 gcggttacaa gcttgaacgc ggctaccagt attgcaacct cgccgcctac catctggccg
      361 acggtgccgc cgaccaggcg ctggaagcgc tgctcgaggc gattgaatcg gttccgcgcg
      421 acgacacatt ctggcactgg gccatgctgc agaatgcggc gggaatagaa gccgctgccg
      481 gcgatccgcg gcgggcggcg cggctcatgg gatttgtcga ccggcgctac ggcgactttc
      541 ccgatggccg ccagcacacc gagcagcgac aacgggcccg catcatggag cagcttgcgg
      601 cggcaattcc cgccgcggaa ctggcgagcc tgatgaagga aggtgaaacg atcagcgcct
      661 tcgaggcgga ctacttcgcc aattttcccg tcctggaact cgatggcgcg gcatagcacg
      721 tttccttccc ggcggttctg gtgtattggc ggattccatg caggacggca gtgacatttc
      781 ccatcagccc gggcggcagc gctggggcac gcgcggcacg gcgggcgcgt tgtttgcggg
      841 caggctcacc tttgagaatg tctcctgcag catcggctcc accgagatcc tgaaggacct
      901 gagctttgaa ctgcaggctg gcgaaatcgc ctgcctgctg ggccagtccg gctgcggcaa
      961 gaccacgctg ctgcgaatcg ctgcgggcat tcaaaggcca acggctggcc gggtgctgct
     1021 tgatggcgag gaagtggatg gtcccacgcg ctatgttccg ccggagcggc gcaatgtggg
     1081 tctggtgttc caggacttcg cgctgtttcc gcacctcacg gtcattgaga acgtcgcctt
     1141 cgggctgagc gccctgagcc gcggggaggc cgccaaggtg gccgaacatg ccctggcgcg
     1201 ggtggggctg gccagtttcc gcgacagtta tcccagctca ttgtcgggtg gcgagcagca
     1261 gcgcgtggcg ctggcgcggg cgatcgtgcc gcggccgcag gtgatgctga tggatgaacc
     1321 cttttccggg ctggaccagc gcctgcgtga aacggtgcgc ggcgaaaccc tggcgctgct
     1381 ccaggaaacg cgggctacct gcgtgctggt gacccatgat ccggtggaag ccatggattt
     1441 cgccgaccgc atcctgctca tgcgggcggg ccggctgatc cagactggct cctcagtgga
     1501 cctgttcaac aggccggcgg acttgcaggt ggcgcggttt ttttccgatg ttaatgaact
     1561 gggcgccatc gttaagaacg gcaatgttga aacgccgctg ggcaccttca gcgctgcggg
     1621 ttttgaccag gggacggagg tcgtggtcat tgtcaggccg caggccctgt ccctcgccca
     1681 ggcgggggca ggaaccgaag gttatgtgct ggaagcccgc tttctggggg acaagaccag
     1741 gctgcaggtg cagtttaatg gcgtggatga gcccctagcc gtgctgcttt cggggctttc
     1801 gccgcaacgg ggcgaaactg cccatttccg cgttgatcac aatcacgttt taatctttac
     1861 aaagcccgcc ccagacagca tttgacaggg tgtgtggcat ttgttgcggg agcgaaagct
     1921 ttctgcgata gtgcctgaca aattgaacat gatgatttca ggagtttggt gatgggtttc
     1981 ggttcgtggt ggcactgggt gcttttgctg attgtcgtgc tggtggtttt cggcggcaaa
     2041 ggcaaggttt cggatttgat gggcgatgtg gccaaaggca tcaagagctt caagaagggc
     2101 ctggcggatg atccggccga ggcgattgct gaagaggtga tgtcgcttga gaagacggcg
     2161 gaagaacccg tcaagtcgcc tgcaaagaag gtcaagtcct aggacttcca agaggttcca
     2221 tgtttgactt tggcgtcggc tattccgaat tgtttgttct tgccctgatt gcggtcatcg
     2281 tcattgggcc caaggatctg ccaaaggtgc tgcgggcctt cggcaagatg atgaccaagg
     2341 cgcggggcat ggcgcgggag ttccagggcc atgtggattc ggccatgaag gatgcg
//
LOCUS       DAORVN010000321         2391 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_44271_length_2391_cov_2.565925, whole genome shotgun sequence.
ACCESSION   DAORVN010000321 DAORVN010000000
VERSION     DAORVN010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2391
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44271_length_2391_cov_2.565925"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..150)
                     /locus_tag="PKV23_07040"
     CDS             complement(<1..150)
                     /locus_tag="PKV23_07040"
                     /inference="COORDINATES: protein motif:HMM:NF015735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HQX85224.1"
                     /translation="MLFALICTDKPNSLELRQQSRPDHLKFLESLGNSLKAAGPFTDD
                     EGRPTG"
     gene            complement(160..1065)
                     /locus_tag="PKV23_07045"
     CDS             complement(160..1065)
                     /locus_tag="PKV23_07045"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008195662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HQX85225.1"
                     /translation="MKRRVAIAGSGAWGQALAHVARTAGHDVSMWSRQKSDVVSFASL
                     DAVIVAVPAQAVREVLTLIKPPKSIPLIIGAKGIEQQTAKFMNEVATEAVPGAHTLVL
                     SGPSFAGDVVKGLPTAVTLAAPTLAEAGEWAQALSLPTFRVYGSDDVLGVEIGGALKN
                     VLAIACGISDGKGLGDSARAALTTRGFAELMRFGKKLGAKPETLTGLAGLGDLLLTCS
                     SRQSRNYSFGLAIGQGAPVSAALAAARGVVEGAYTARVAQALAVRHEVDMPIVAAVSA
                     IIDQGAEPAQEIARLLARPVREEIR"
     gene            complement(1062..>1522)
                     /locus_tag="PKV23_07050"
     CDS             complement(1062..>1522)
                     /locus_tag="PKV23_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012091158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HQX85226.1"
                     /translation="KDCNFSFSGLKTAVREAVQNLALLSEQDIADVCASFELAVAETL
                     QDRLKMAFATFRETHPEAMEPALIVAGGVAANMTLKTVLAASALESGFKLIVPPASLC
                     TDNGAMIAWAGAERLTLGLVDDLNFMARARWPLDEVSTPVYGSGKLGAKA"
     assembly_gap    1523..1532
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1533..2138)
                     /gene="tsaD"
                     /locus_tag="PKV23_07055"
     CDS             complement(<1533..2138)
                     /gene="tsaD"
                     /locus_tag="PKV23_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012091158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HQX85227.1"
                     /translation="MHILGIETSCDETAAAIIRRNADGSGEIVSNIVLSQIADHAPYG
                     GVVPEIAARVHIKHLDRLIAQAMDEAGIAFPDLDGVAATAGPGLLGGVIIGLTTAKAI
                     AMAHGKPLLAINHLEGHALTPRLLEPCPFPYLLLLISGGHTQFQLVEGVGRYRRLGTT
                     IDDALGEAFDKTAKLLGLGYPGGPLVEQFARKGNARRFDFPR"
     gene            2229..>2391
                     /locus_tag="PKV23_07060"
     CDS             2229..>2391
                     /locus_tag="PKV23_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018700087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HQX85228.1"
                     /translation="MPRIKIGTRGSPLALVQARETRDRLIAAHGLDPSEIEIVSIATT
                     GDRIKDRPLN"
BASE COUNT          500 a          787 c          656 g          438 t
ORIGIN      
        1 gccggtgggc ctgccctcat catcggtgaa agggccggcg gccttcaggc tgttgccgag
       61 gctttccaga aacttgagat ggtcaggccg cgattgctgg cgcaattcca aactgttcgg
      121 cttgtcggtg catatcaacg caaaaagcat gtcttctccc tatcttattt cttcgcgaac
      181 cggtcgcgcc agaagcctgg cgatttcctg ggccggttcc gcaccctgat caatgattgc
      241 cgaaaccgcg gccacgatgg gcatgtcaac ctcatgcctt acggcaagtg cctgtgcaac
      301 tctggccgta tacgctccct caacaacgcc gcgcgctgcc gccagcgccg cactcaccgg
      361 agcgccctgg ccgatggcaa ggccaaagga atagttgcgc gactgacggc tggagcaggt
      421 gagaagcagg tcaccgagcc ccgctagccc cgtcaatgtc tcaggctttg ccccaagttt
      481 cttgccaaag cgcatcagtt ctgcaaagcc ccgcgttgtc agcgctgccc gcgcgctgtc
      541 cccaaggccc ttgccatcgg aaatgccgca ggcaatcgcc agaacatttt ttaaagctcc
      601 cccgatttca acgccaagaa catcgtcgga cccgtaaacc cggaacgtcg gaagcgaaag
      661 ggcctgggcc cattcgccgg cctccgccag ggtaggcgcc gcaagtgtca cagcggttgg
      721 caggcccttt acaacatccc ccgcaaaact cgggccggaa agcacaagcg tgtgcgcccc
      781 cggcacggct tccgtcgcca cttcattcat gaacttggcc gtctgctgtt caatgccctt
      841 ggcgccgatg atcaggggaa tgcttttagg cggcttgatc agcgtcagca cctcgcgcac
      901 tgcctgcgcc ggaactgcca caatgacagc atcaaggctg gcgaacgaaa ccacatcgga
      961 tttttgccgc gaccacatgc tgacgtcatg gccagcagtc cttgcgacat gagccagcgc
     1021 ctggccccag gcgccactcc ccgcaatggc gacacgccgc ttcatgcctt ggcgcccagc
     1081 ttgcccgagc cataaaccgg ggttgaaacc tcatccagcg gccagcgggc ccgcgccatg
     1141 aaattgagat catcgacaag gccaagggtc aggcgttccg ctcccgccca cgctatcatc
     1201 gccccattgt cagtgcaaag gctggccggc ggcacaatga gcttgaaacc agactctagg
     1261 gccgacgcgg cgagaactgt tttcaacgtc atgttcgccg caacaccgcc cgccacaatc
     1321 aaggcaggct ccatcgcctc cggatgggtt tcccgaaaag tcgcaaaagc catcttcaat
     1381 ctgtcttgca gcgtttcagc caccgccagt tcaaagctgg cgcagacatc tgcaatatcc
     1441 tgttccgaca gaagtgccag attttgaact gcctcgcgca ccgcggtttt caaacccgaa
     1501 aaggaaaaat tgcagtcttt ccnnnnnnnn nngcgtggaa aatcaaaacg ccgcgcattt
     1561 ccttttcgcg caaattgttc caccagcggg ccgccgggat aacccaggcc cagtagcttt
     1621 gccgtcttgt cgaaagcctc gcccagggca tcgtcgatgg tggtgcccag ccggcggtag
     1681 cgccccacac cctcaaccag ttgaaactgc gtgtgcccgc cggaaatcag caggagcaga
     1741 tagggaaacg ggcaaggctc cagcaggcgc ggcgtgagcg cgtggccctc cagatgattg
     1801 atagcgagca aaggcttgcc atgggccatg gcaatggcct tggccgtcgt gaggccgatg
     1861 atgacaccgc ccaaaagccc cggccctgcc gttgccgcca cgccgtcaag atcgggaaaa
     1921 gcgatccccg cctcatccat ggcctgcgcg atcaggcggt ccagatgctt gatgtggacc
     1981 cgcgccgcga tttccggcac caccccgcca tagggcgcgt ggtccgcaat ctgcgaaagc
     2041 acgatattgc tgacgatttc gccgcttcca tcagcattcc ggcgaatgat ggcggcagcc
     2101 gtttcatcac aggacgtctc aattccgagg atatggatag ctgctacggc catgggtaat
     2161 ggatgacgtc tcacaattta gcggctagaa cgggccccgc tctagcatcc accggataca
     2221 acttgcaaat gccgcgaatt aagatcggaa cccgggggtc gcccctcgcc cttgtccagg
     2281 cccgcgaaac ccgcgaccgc ctgattgccg cccatggcct ggatccatcc gagattgaaa
     2341 ttgtctcgat tgcgacgaca ggcgaccgca tcaaggaccg cccgctgaat g
//
LOCUS       DAORVN010000322         2386 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_44453_length_2386_cov_3.083226, whole genome shotgun sequence.
ACCESSION   DAORVN010000322 DAORVN010000000
VERSION     DAORVN010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2386)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2386)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2386
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44453_length_2386_cov_3.083226"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..439)
                     /locus_tag="PKV23_07065"
     CDS             complement(<1..439)
                     /locus_tag="PKV23_07065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85229.1"
                     /translation="MNIAATIGHNNPPDPIDTITAPFDDFRTEAEAWLDGTKVENEDQ
                     MKAADKLRGSMRDWRLALEKGQKSATAPLHDIYKAELERWKPTIEDARRIEAGLVGIL
                     DGFKRKLAAEKAEAERIAQAAAWEATRKAQEAARLADATNIEAT"
     gene            complement(451..732)
                     /locus_tag="PKV23_07070"
     CDS             complement(451..732)
                     /locus_tag="PKV23_07070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85230.1"
                     /translation="MILQSLNISLKMPYSKPGPSNPYQAKLSVSYNENTMQVQLSSET
                     CERILALAGDEIATAAQIQIADFVQTALAISRLPQIEGVAIASPEPMPF"
     gene            complement(744..1451)
                     /locus_tag="PKV23_07075"
     CDS             complement(744..1451)
                     /locus_tag="PKV23_07075"
                     /inference="COORDINATES: protein motif:HMM:NF016302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ERF family protein"
                     /protein_id="HQX85231.1"
                     /translation="MTAQETITAGVAVSAQSGIISAVAKAMGEMKRIAKDSRNIEQKY
                     DFASVDDFLAMTGPICAANGIITILDEEAVEDFERQGKYGVTHWLRIRFAISTMHTSG
                     ETLPVVRRTVEVIRTGAQSFGSAQSYVLKQYQRALYQIPTGDKDDADFAEKGDGPATK
                     AQPKQDHTPRDNGPTAAQIACDSLTNADTLEALGAIWQGLPVSIKGEATVFKAKDDRK
                     AALQPKQNADLADEIPY"
     gene            complement(1566..1877)
                     /locus_tag="PKV23_07080"
     CDS             complement(1566..1877)
                     /locus_tag="PKV23_07080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85232.1"
                     /translation="MSAPKTIWAFHDGPMVGLFIGKKEVGSNIAEYTLTDPTTITLDR
                     AEYEALVEALEFYADKSGWNQPPVKTRDSLISVEYENQASKIQRDRGRIAVAALKAVQ
                     S"
     gene            complement(1874..2143)
                     /locus_tag="PKV23_07085"
     CDS             complement(1874..2143)
                     /locus_tag="PKV23_07085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85233.1"
                     /translation="MTIHTFPGAAPARRVLSGNTASEEQRRIALAMINDSFRYGPLPD
                     AHNCELLADMNAKRAQSEPVPLAGVLLIMSAFGFFGAALAVWVLV"
     gene            complement(2140..2334)
                     /locus_tag="PKV23_07090"
     CDS             complement(2140..2334)
                     /locus_tag="PKV23_07090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85234.1"
                     /translation="MSMTANLHDAVSAEATIVDRGTSWLTIKAKNGDYVSIFMPYATA
                     QAMAAAFNAPIAAPVLEKAQ"
BASE COUNT          441 a          664 c          689 g          592 t
ORIGIN      
        1 gtgtggcttc gatattggtg gcatctgcca gccgtgctgc ctcttgcgcc ttgcgggtcg
       61 cctcccatgc agcggcctgt gcgatgcgct cggcctcggc cttttccgct gccagcttgc
      121 gcttgaaccc gtcaaggatt ccaacaagcc ccgcctcgat ccgcctggcg tcttcgatgg
      181 tcggcttcca gcgttccagt tcggccttgt aaatgtcatg cagcggggcc gtggcggact
      241 tctggccttt ttccaaagcc aagcgccaat cgcgcattga tccgcgcaac ttgtctgcgg
      301 ccttcatttg gtcttcgttc tcgaccttcg ttccgtcaag ccaagcctcg gcctcggtgc
      361 ggaaatcatc aaacggcgcg gtgatggtgt cgataggatc gggtgggttg ttgtgaccga
      421 tggttgctgc gatgttcatt ttgctcacca tcaaaacggc atgggttcag gcgaggcaat
      481 cgcgacaccc tcgatctgcg gtagtctgga aatagccagc gcagtctgaa caaaatcagc
      541 gatctgtatt tgcgcagcgg tggcgatttc atccccggca agtgccaaaa tgcgctcgca
      601 agtttcgctg gaaagctgaa cctgcattgt gttttcgttg tagcttacgc tcaactttgc
      661 ttgatagggg ttgctcggcc ccggcttgct atacggcatc ttgagcgaaa tattcaggct
      721 ttgaaggatc atggctctat ctcctaataa gggatttcat ccgccaaatc ggcgttctgt
      781 ttcggttgca gcgcggcctt gcggtcgtct ttggctttga aaaccgttgc ctcgcccttg
      841 atcgaaacag gcaagccttg ccaaatcgcg ccaagggctt cgagcgtgtc ggcattggtc
      901 aggctatcgc aggcgatctg cgccgccgtg gggccgttat cgcgcggggt gtggtcttgc
      961 ttgggctggg ccttggtcgc tgggccgtcg cctttttcgg caaaatccgc gtcgtcttta
     1021 tcgcccgttg ggatctgata cagtgcccgc tgatattgct tgagaacgta ggattgcgca
     1081 gagccaaacg attgcgcccc agtgcggatc acctcaacag ttctgcggac tacaggcagg
     1141 gtttcgccgc ttgtgtgcat ggtcgagatc gcaaaccgga tgcgcagcca atgggttacg
     1201 ccatactttc cctgacgctc aaaatcctca accgcttctt cgtcgaggat ggtgataatc
     1261 ccgtttgctg cacagattgg tcctgtcatt gccagaaagt cgtcaacgct ggcaaagtca
     1321 tacttttgct caatgttgcg gctatccttg gcgatgcgct tcatttcgcc catagccttt
     1381 gcaacggcgg aaatgatccc ggattgtgca gataccgcaa cgcctgcggt gatggtttct
     1441 tgagcggtca tgttagtaag cctccgcaat ctgaacggtt gttgtaatgc cgcccaccgg
     1501 gtcaacatgg gccagccatg cgaatagccc gatgcacgcg gcgagggtca ggagggtttc
     1561 gcgggtcatg attgcaccgc ctttagtgcg gcaacggcga ttcttccccg gtcacgctgg
     1621 atttttgatg cttggttctc atattcaacg gaaatcaaac tatcacgcgt tttgactggt
     1681 ggttggttcc atccgctttt atcagcatag aactcaagcg cctccaccag cgcctcatac
     1741 tccgcccggt cgagcgtgat ggtggtgggg tcggtgaggg tgtactcggc aatattgctg
     1801 cctacctctt ttttgccaat aaatagacct accatcggcc catcatggaa ggcccagatc
     1861 gttttcggcg cgctcatacc agcacccaca cagccaaagc cgcgccgaaa aagccaaaag
     1921 ccgacatgat caacagcacg ccagcaagcg ggacaggctc ggactgcgcg cgctttgcat
     1981 tcatatccgc cagcagttcg cagttgtggg catcaggcag cgggccatag cggaagctgt
     2041 cattgatcat ggcaagggca attcggcgct gttcttcgct tgcggtgttg ccagagagaa
     2101 cgcggcgggc cggggcagcg cctgggaatg tgtgaatggt cattgggctt tctccagaac
     2161 gggcgctgca atcggcgcgt tgaaggctgc ggccatggct tgtgccgtgg cgtagggcat
     2221 gaagatcgac acataatcac cgttctttgc ctttatcgtc agccatgaag tgcctcggtc
     2281 aacgattgtg gcctcagccg aaactgcatc gtgaaggttt gcggtcatgc tcattttgag
     2341 tctccatcaa gccgcgcttt gcgggctgtt gctgcgagga tcaggg
//
LOCUS       DAORVN010000323         2379 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_44694_length_2379_cov_2.466437, whole genome shotgun sequence.
ACCESSION   DAORVN010000323 DAORVN010000000
VERSION     DAORVN010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2379)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2379)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2379
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44694_length_2379_cov_2.466437"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..557
                     /locus_tag="PKV23_07095"
     CDS             <1..557
                     /locus_tag="PKV23_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011995988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HQX85235.1"
                     /translation="NKYDDAINAANRFITLHPGHKDSAYAYYLTAISQYEQISDVRRD
                     QSRTQKALEALEEVGKRYPQSPYAADAMKKADMARDHLAAKEMEVGRYYLKKGSYLAG
                     INRFKKVVTDYQTTSQVPEALYRLSEGYMALGVVSEAQTAAAVLGQNFPNSEWYKDAY
                     TLVSSDGQQPVENKKSWISRLFSS"
     gene            603..2270
                     /gene="recN"
                     /locus_tag="PKV23_07100"
     CDS             603..2270
                     /gene="recN"
                     /locus_tag="PKV23_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009799270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HQX85236.1"
                     /translation="MLTALSIRDIVLIEKLDISLESGLTVLTGETGAGKSILLDALGL
                     ALGGRGDSGLVRHGAAKGVVTAGFVLPKAHAALRLLEEQGIETEGELVIRRIQNADGP
                     SRATSNDQPVSLNLLRQIAGSLAEIHGQHADRALVDVANHRRLVDAFGDLEGQAGEVA
                     KLWQALSDLTNELAEHRALMARAEAERDYIEHAVEELRVLDPQPDEELMLASARQVMM
                     HAEQYAGALSEAESVLSGDSVEARLNAALRKLERRKDTAGGRLDQACAALDKVLIEMN
                     EANRALAEAQRSFSFNPADLEKSEERLFALRAAARKHKCTVDALAATRERFEKELATL
                     TDGGQQLAKLEARTAAAQKDYDKAADLLSADRKKVAKNLDKAVQAELPPLKLAKARFE
                     TQITSDAGKPGPHGIDRVEFVVAANPGTPLSPLMKVASGGELARFMLALKVILAAKGS
                     APTLIFDEIDTGVGGAVADAIGGRLARMAQGLQVLAVTHSPQVAARASEHMLISKMEE
                     VGSHRMVTRVQSLSEKSRREEVARMLSGAQVTDEARAQADRLLAAKG"
BASE COUNT          513 a          664 c          806 g          396 t
ORIGIN      
        1 acaataaata cgacgatgcg atcaatgccg ccaaccggtt catcaccctg caccccggcc
       61 acaaggattc ggcctacgcc tattacctga cggcgatttc acaatacgag cagatcagcg
      121 acgtgcgacg cgatcagtcg cggacgcaaa aggccctgga agctctcgaa gaagtgggca
      181 aacgttaccc gcagtcaccc tatgcggcgg acgcgatgaa gaaagctgac atggcgcgcg
      241 accatcttgc cgccaaggaa atggaagtcg gacgctatta cctcaagaag ggctcctatc
      301 ttgcgggcat caaccgcttc aagaaagtgg tgaccgatta ccagacgacc agtcaggtgc
      361 cggaagcgct gtaccgcctg agcgaaggct acatggcgct gggcgttgtt tcggaagcgc
      421 agacggcggc tgccgtgctg gggcagaatt ttcccaacag cgagtggtac aaggatgcct
      481 acacgctggt atccagtgat ggccagcagc cggttgaaaa caagaaatca tggatcagcc
      541 gcctcttcag cagctaattt aggctctcgc gcttcgccaa aagactcagc tatagtctgg
      601 tcatgctgac cgccctttcc atccgcgata tcgtgcttat cgagaagctc gacatttcgc
      661 ttgagagcgg cctgacggtt ttgacgggcg aaaccggcgc cggaaaatcc attctgctgg
      721 atgcgttggg cctggccctt ggcgggcgcg gcgactcagg cctggtgcgc cacggagccg
      781 ccaagggcgt ggtcaccgca ggattcgtgc tgcccaaggc ccatgcggcg ctgcgcctgc
      841 tggaagagca gggcatagaa acggaaggcg agctggtgat acggcgcatc cagaatgccg
      901 atggcccgag ccgcgccacg agcaacgacc agccggtgag cttgaacctg ctgcgccaga
      961 tcgccgggag ccttgctgaa attcacggcc agcatgcgga tcgggccctt gtggacgtgg
     1021 ccaatcaccg gcggctggtg gatgccttcg gtgatctgga aggacaggcc ggcgaagttg
     1081 caaaactctg gcaggcgttg agcgatctca cgaatgaact ggccgagcat agggcgctga
     1141 tggcgcgggc ggaggccgaa cgcgactaca tcgaacatgc ggtggaagaa ttgcgggtgc
     1201 tcgatccgca gccggatgag gaactgatgc ttgccagcgc gcgccaggtg atgatgcatg
     1261 cggagcaata tgcaggcgcg cttagcgagg ccgaaagcgt gctgtccggc gacagtgtcg
     1321 aagcgcggct gaatgcggct ctgcgcaagc tggaacggcg caaggataca gcgggcggca
     1381 ggctggacca ggcctgcgcg gcgctcgaca aggtgctcat tgaaatgaac gaggcgaacc
     1441 gggcgctggc cgaggcgcag cgttccttct cgttcaatcc ggcggatctg gaaaagtccg
     1501 aagagcggct gttcgcattg cgggcggcgg cgcgcaagca caaatgcacg gttgatgcgc
     1561 tggcggcaac gcgcgagcgg tttgaaaagg aactggcgac gctcaccgat ggcggccaac
     1621 agctagcgaa gcttgaggcc cggactgctg cagcacagaa ggactatgat aaggcagccg
     1681 atctgttgag tgcggaccgc aagaaggttg cgaaaaatct ggacaaggcc gtgcaggcgg
     1741 aactgccgcc gctcaagctg gcgaaggcac ggtttgaaac gcagatcacc agcgatgcgg
     1801 gcaagccggg accgcacggc attgaccggg ttgaatttgt cgtggcggca aatcccggaa
     1861 cgccgttatc gccgctcatg aaggtggctt cgggcggtga gctggcgcgc ttcatgctgg
     1921 cgctcaaggt gatccttgcg gccaagggct cggcccccac tttgattttc gatgaaatcg
     1981 atacgggagt tggcggggcc gtcgccgatg ccatcggcgg acgcctggca cggatggcgc
     2041 agggcctgca ggttctggcg gttacccatt caccgcaggt ggcggcgcgg gccagcgagc
     2101 acatgctgat ttcaaagatg gaagaagtgg gcagccaccg catggtgaca cgggtgcaat
     2161 cgctgtcgga gaagagccgg cgcgaggaag tggcgcgcat gctgtcgggc gcgcaggtga
     2221 ccgatgaggc gcgcgcccag gccgaccgct tgctggcggc caaaggctga ttcatgccgg
     2281 agaaatcccc tgaccagttg agtttggcgg aggccaagtc ggagttgaaa cgccttgcca
     2341 gggaaattgc cgaacatgat gagcgctatc acggccagg
//
LOCUS       DAORVN010000324         2376 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_44789_length_2376_cov_2.856097, whole genome shotgun sequence.
ACCESSION   DAORVN010000324 DAORVN010000000
VERSION     DAORVN010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2376
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44789_length_2376_cov_2.856097"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(22..1224)
                     /locus_tag="PKV23_07105"
     CDS             complement(22..1224)
                     /locus_tag="PKV23_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007599016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyltransferase family protein"
                     /protein_id="HQX85237.1"
                     /translation="MNDPRLHFKKPLLTSPFHARTSALNKLNSWGPWGGYTTVLAYDD
                     ESMEYTAIRNQASVYDLSPMVKYKIAGKDAEAYLDRLMVRNVAKLNVGHVHYTAWCDD
                     EGKLLDDGTLFRRAASHFMLCCQERHLPWLLDSAIGYDVAVSEISEEIAALSLQGPCS
                     YSVLKAAGFEKAAALKPFQMADFRFGKSGALTISRTGFTGDLGYELWTSPARALELWD
                     LLFTAGALYGIRPIGTNALNMARIEAGFIIANMDFITAEHAVRGDRARSPLEMGLEWM
                     IDFDKGHFNGRRTLVEEKAKGTSKWALVGLDIEGNVSAEHSLIYHNKKDEVGHITAAL
                     WSPATKRNIALAMMERPYHAERSNDLWVEIYASRELQYHKLMVKARVTPRPFLNLARR
                     RAAPPLNY"
     gene            complement(1221..>2376)
                     /locus_tag="PKV23_07110"
     CDS             complement(1221..>2376)
                     /locus_tag="PKV23_07110"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HQX85238.1"
                     /translation="RFANVLKPFKAMTPPRLGKGVGNEMLKLAGLGLKIRGLGRDDLR
                     EFMRLMLINVADVLDDELKDERLKGVVAFDTVLGAHLGPRSPNSLLLLLNRLAGETLS
                     LPAGGMGSVAAAMAKAVQALGVDIRLNAKVKSVNIVGDRATGVTLQNGDVIDATTVIS
                     AINPKTTFLDLVGPRHLDTGMVRRAGNILMRGNAVKLHLALKGAPNFNDADLKTRLVI
                     APSVRVVEDAFNPVKYGEFSHDPVMEIVIPSAFEEGFAPKGHHVLSAIVQYAPVVMDA
                     KARAAFLKRIMATLESYAPGIGKLVVKSELLTPENLENQYGFVGGNWHHGELAAEQML
                     FLRPFIGAAQYETAIPGLWLAGAGSHPGGGISGAAGWNAAERILKMEGRR"
BASE COUNT          463 a          666 c          772 g          475 t
ORIGIN      
        1 tccgtggcct gatgccactt ttcaataatt caagggcggc gcggccctgc ggcgcgccag
       61 attcaagaag ggccgcgggg tgacccgggc tttaaccatg agcttgtggt actgcagttc
      121 gcgggaagca tagatctcaa cccagagatc attgctgcgc tcggcatggt agggacgttc
      181 catcatcgcc agggcgatgt tgcgcttggt ggccggcgac cagagggcgg cggtgatgtg
      241 acccacttcg tccttcttgt tgtgatagat cagcgaatgt tcggcggaga cattaccttc
      301 aatatcaagg ccgacaaggg cccatttaga ggtgcctttg gccttttcct caaccagggt
      361 gcgcctgccg ttgaaatggc ccttgtcgaa atcgatcatc cattccaggc ccatttcgag
      421 gggcgacctt gccctgtcgc cgcgcacggc gtgttcggcc gtgatgaaat ccatgttggc
      481 gatgatgaag cccgcctcga tgcgcgccat gttcagcgca ttggtgccga tggggcggat
      541 gccgtagagg gcgcctgcgg tgaacaggag atcccagagt tcgagggcgc gggccggcga
      601 ggtccacagt tcatagccga ggtcgccggt gaagccggtg cgcgagatgg tgagcgcgcc
      661 ggatttgcca aatctgaaat cggccatctg gaagggcttg agggccgcgg ctttctcaaa
      721 ccccgcggct ttcaatacgg aataggagca tgggccttgc agcgaaagtg cggcgatctc
      781 ctcactgatt tcgctgacgg ccacgtcata gccaatggcg ctgtcgagga gccacgggag
      841 gtggcgttcc tggcagcaga gcatgaagtg ggaagcagca cggcggaaca aggtgccgtc
      901 gtccagcagc ttgccttcgt catcgcacca ggcggtgtaa tggacgtggc ccacattcag
      961 cttcgccaca ttgcggacca tcaggcggtc gagataggct tcggcatctt tcccggcgat
     1021 tttgtatttc accatgggcg agaggtcata aacgctggcc tggttgcgga tcgcggtata
     1081 ctccatggac tcgtcgtcgt aggcgagcac cgtggtgtag ccgccccagg ggccccagga
     1141 gttgagcttg ttgagcgccg aagtgcgggc atggaagggc gaagtgagca gcggtttctt
     1201 gaaatggaga cggggatcgt tcatcgcctg ccctccatct tgaggatgcg ctctgccgca
     1261 ttccagcctg ccgcaccgga aataccgccg ccgggatggg atcccgcgcc ggccagccag
     1321 aggccgggga tggcggtttc atactgggcg gcaccgatga agggcctcag gaaaagcatt
     1381 tgttcggcgg cgagttcgcc gtggtgccag ttgccgccga caaagccgta ttgattttcg
     1441 agattctctg gggtgagcag ttcggatttg accacgagct tgccgatgcc cggcgcatag
     1501 gattcgagcg tcgccatgat gcgtttcagg aaggcggcgc gggccttggc atccatgacg
     1561 accggggcat actgcacgat ggccgagagc acatggtggc ctttgggggc gaagccttcc
     1621 tcgaaggccg agggaatgac aatctccatc acaggatcgt gtgagaactc gccgtatttc
     1681 accggattga atgcatcttc cacaacgcga accgatggcg caatcaccaa acgggtcttg
     1741 aggtcagcgt cattgaagtt gggcgcaccc ttgagggcga gatggagctt caccgcattg
     1801 ccgcgcatca ggatattgcc ggcgcggcgg accatgccgg tatcaagatg gcgcgggccc
     1861 acgagatcaa ggaatgttgt cttgggattg atggcggaga tcacggtcgt ggcgtctatc
     1921 acgtcaccgt tctgcagggt cacacctgta gccctgtcgc ccacgatgtt cacggatttc
     1981 actttggcat tgaggcggat atcaacgccc aaggcctgca cggccttggc catggcggcg
     2041 gccacggagc ccatgccgcc tgcgggcaat gacagggttt cgcccgcgag cctgttcaaa
     2101 agcagcagca gcgagttggg cgagcgcggg ccgagatggg cgcccagcac cgtatcgaag
     2161 gcgacgacac ctttcaggcg ctcatccttc aattcatcat cgaggacatc ggccacattg
     2221 atcagcatga ggcgcatgaa ttcgcggaga tcgtcacggc caaggccacg gattttgagg
     2281 cccaagcctg cgagtttcag catctcattg ccaacaccct tgccgaggcg cggcggggtc
     2341 atggccttga aaggcttcag tacattcgca aaacgc
//
LOCUS       DAORVN010000325         2370 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_44985_length_2370_cov_2.501944, whole genome shotgun sequence.
ACCESSION   DAORVN010000325 DAORVN010000000
VERSION     DAORVN010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2370)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2370)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2370
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44985_length_2370_cov_2.501944"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(159..623)
                     /locus_tag="PKV23_07115"
     CDS             complement(159..623)
                     /locus_tag="PKV23_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006727251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HQX85239.1"
                     /translation="MTGDVPFWKTKTLTDMSRDEWESLCDGCGRCCLNKLEDEDTGKF
                     LYTRAACKLLDVKTCRCTDYPNRAARVPDCVTLTPENVGELGWLPQTCAYRLLDEGKS
                     LPWWHPLVSGRPETVIEAGISVSSEAYSEEGITVEQLLDHLWKLPKAKRRSL"
     gene            753..>2370
                     /locus_tag="PKV23_07120"
     CDS             753..>2370
                     /locus_tag="PKV23_07120"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HQX85240.1"
                     /translation="MAKLPNRNPIFRVFWAFDSLVSSSVFETWDACKRWGSAYSSFVY
                     RFKITGPRRFVVDIVDDFTTFGMVFAFCLLAFALPPFSGTGDVWNRGREFAITFTDAN
                     GEIIGQRGIRQDDAIPLEDIPPSLIKAVLATEDARFFEHFGVDVIGTMRAIIQNARAN
                     TVVQGGSSLTQQVAKNLFLSPERTIRRKVHEAFLAIWIEARLSKQEILKLYLDRSYLG
                     GGNYGVEAAAQFYFGKSIRDVTLSESAMLAGLFKAPSKYAPHQNMEVAKARANVVLYR
                     MLDAGFITQGELLQARRQAPPVIAQRSLDSPDWFLDWAYEQTIALLEEQRLLSDFVIE
                     VKTTVDVKVQAAAQRILNEIIDTEGPGYRFTQMASVTMAPDGAVKAIVGGRNYETSQF
                     NRATNAERQTGSAFKPFVYLAALLEGYTPDRMVVDAPISVGGWSPGNYSGKYGGRVTM
                     TTALAKSYNSVPVRLMVDIGRPAIIKTTHDVGVKGELETWPPMVLGTSSLTLMDVTTG
                     YATFAGGGKLAEPYTILEIKGPMATSSMTAP"
BASE COUNT          474 a          715 c          692 g          489 t
ORIGIN      
        1 cagttcgcga gtgagcggca caagagtgtt gacaccgtgc gcgacatgat gaagggccat
       61 gcaaacctca caggcccgcc cgtgcgcgcc ctgtcctgtg tgattctttg agaagcatac
      121 agtcctgtca agccaatgcc tgagagagcg cacgcaaatt aaagtgatct gcgcttcgcc
      181 ttgggcagtt tccagaggtg atcgaggagc tgctccacgg tgatgccctc ttccgaatag
      241 gcttcgctgc tgaccgagat cccggcctcg atcacggtct caggccttcc cgagaccagc
      301 gggtgccacc aggggaggct ctttccctcg tcgagcaggc ggtaggcgca ggtttgcggc
      361 agccagccaa gctcgcccac gttctcaggc gtcaatgtta cacaatcggg cacccttgcg
      421 gcgcgattgg gataatcggt gcagcggcag gttttgacgt caagcagctt gcaggcggcg
      481 cgggtgtaaa ggaatttgcc ggtatcctcg tcttccagct tgttgaggca gcagcggccg
      541 cagccatcgc agaggctttc ccactcgtcc cggctcatgt cggttaatgt cttggtcttc
      601 cagaagggaa catcgcctgt catggccccc tcttaatcct tcccatgccc catgagcaat
      661 tgccatgcgg cgtgattttg cctaaatcgc gccctaatct gctatacaga cagcagtcgc
      721 aggatcacaa cgagagtcta acggtcaaaa acgtggcaaa actacccaat cgcaacccca
      781 ttttccgtgt tttctgggcc ttcgactcgc ttgtgagctc gagcgtcttt gaaacctggg
      841 atgcgtgcaa gcgctggggt tcggcctatt cctcgtttgt ttaccgcttc aagatcacgg
      901 gcccgcgccg cttcgtggtg gacattgtcg atgattttac aacattcggc atggtgtttg
      961 ccttctgcct cctggctttt gccctgccgc ctttctcggg caccggtgat gtgtggaacc
     1021 gcggccgcga gtttgccatt acctttactg atgccaatgg cgagatcatc ggccagcgcg
     1081 gcatccggca ggacgacgcc attccgcttg aagacattcc gccctccctg atcaaggcgg
     1141 tgcttgccac ggaagacgcc cgcttcttcg agcatttcgg ggtcgacgtc atcggcacga
     1201 tgcgcgcgat catccagaac gcccgggcca acaccgtggt gcagggcggc tcgtcgctga
     1261 cccagcaggt cgccaagaac cttttcctca gccctgaacg caccatccgc cggaaagtgc
     1321 atgaggcctt tcttgcgatc tggatcgagg cgcggctttc caagcaggaa atcctgaaac
     1381 tctatctcga ccgctcctat cttggcggcg gcaactacgg agtggaagcg gcggcgcaat
     1441 tctattttgg caagtcgatc cgtgacgtca ctctgtcgga atcggcgatg ctggcgggcc
     1501 tgttcaaggc gccttcgaaa tatgcaccgc accagaacat ggaagtggca aaggcccggg
     1561 ccaacgtggt gctctaccgc atgcttgatg cgggcttcat cacccagggc gaactgctgc
     1621 aggcacggcg ccaggcgccg ccggtaattg cgcagcggag cctcgacagt cccgactggt
     1681 tcctggattg ggcttacgaa caaaccatcg ccctgcttga agagcaacgc ctgctcagcg
     1741 atttcgtgat cgaagtcaag acaacggttg atgtgaaggt gcaggcggcg gcgcagcgca
     1801 ttctcaatga gatcatcgac acggaaggtc caggctaccg cttcacgcaa atggcctcgg
     1861 tcacgatggc gccggacggc gcggtcaagg ccatcgtagg cggccgcaac tatgaaacca
     1921 gccagttcaa ccgggccaca aatgccgagc ggcaaaccgg atcggcgttc aaaccctttg
     1981 tctatctggc ggcccttctc gaaggataca cgccggaccg catggtggtt gacgccccca
     2041 tatcggttgg cggctggtcg cccggaaact actccggcaa gtatggcggg cgcgtcacga
     2101 tgacaacagc actcgccaaa tcctacaatt cggttccggt ccggctgatg gtggacattg
     2161 gcaggccggc gatcatcaag acaacccatg atgtgggcgt caagggcgag ctggaaacct
     2221 ggccgccgat ggtgcttggc acgagttccc tgaccctgat ggatgtcacc acgggttatg
     2281 ccacgtttgc gggcggtgga aagctggcgg aaccctacac gattctggaa atcaaaggcc
     2341 caatggcgac atcatctatg accgcgccaa
//
LOCUS       DAORVN010000326         2358 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_45387_length_2358_cov_3.746418, whole genome shotgun sequence.
ACCESSION   DAORVN010000326 DAORVN010000000
VERSION     DAORVN010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2358
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45387_length_2358_cov_3.746418"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..514)
                     /locus_tag="PKV23_07125"
     CDS             complement(<1..514)
                     /locus_tag="PKV23_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008872478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HQX85241.1"
                     /translation="MKITVVGTGYVGLVSGACLAEMGNDVLCLDLDPEKIRVLKEGGI
                     PIHEPGLDQVVARNVAAGRLHFTTDVQDAVQFGTIQFIAVGTPPDEDGSADLQYVLSA
                     ARAIGRHMTDYKVVVDKSTVPVGTADKVKAAVQAELDARGEAIPYSVVSNPEFLKEGA
                     AVDDFMRPDRI"
     gene            complement(601..1776)
                     /gene="lapB"
                     /locus_tag="PKV23_07130"
     CDS             complement(601..1776)
                     /gene="lapB"
                     /locus_tag="PKV23_07130"
                     /inference="COORDINATES: protein motif:HMM:NF008755.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HQX85242.1"
                     /translation="MEFELWWLLAFPLFFVLGWLAARIDIKHLVKESRALPRTYLTGL
                     NFLLNEQPDKAIDAFIEAVKIDNQTVELHFALGSLFRRRGETDRAIRMHQNLIDRDDL
                     SDDQRLQALSELGLDFLKAGLLDRAEAVFVQLRGSRLNDFALKNLLDIYQQEKDWRKA
                     IETADALPGHESALWEKEMANFHCELASADMANSRFDEARGALLRALETNRKCVRASM
                     LLGDLYVAQGDDRAALEAWKRVEGQNPVYLALVAERLMDAYRRLGELEQGMQLLRSYL
                     EQYASLDLLDALFQWELQKEGPAAAYQLVRDELRRNPTLLGLDKLLEAALLTAPIEQR
                     TDIELVKGLVHGHTRRVARYRCDACGFKARQFYWRCPACGGWETYPPKRTEEFDLTP"
     gene            complement(1781..2179)
                     /locus_tag="PKV23_07135"
     CDS             complement(1781..2179)
                     /locus_tag="PKV23_07135"
                     /inference="COORDINATES: protein motif:HMM:NF018058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HQX85243.1"
                     /translation="MTLPHPEAEGSILKAAKAAFFFLSTSGIISGMRALIWVFRLFLF
                     LLLFGFAVKNDDVVVLRFFFGAQWQVPLVLVILLFVTIGVLIGVTATLTTLYRQHREI
                     GHLRDQGAATPGEGRGKAPIAHGPDLPETY"
     gene            complement(2186..>2358)
                     /locus_tag="PKV23_07140"
     CDS             complement(2186..>2358)
                     /locus_tag="PKV23_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008810808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HQX85244.1"
                     /translation="RGDRIEIRGFGSFALNYRPPRVGRNPKSGEKVQVPEKYVPHFKA
                     GKELRERVDMMG"
BASE COUNT          525 a          750 c          662 g          421 t
ORIGIN      
        1 cgatccggtc ggggcgcatg aagtcgtcca ccgccgcccc ctccttcagg aactcggggt
       61 tggacaccac actgtaggga atcgcctcgc cgcgcgcatc gagctcggcc tgaaccgcag
      121 ccttgacctt gtcggcagtc ccgacaggca cggtgctctt gtcgacgacg accttgtagt
      181 cggtcatgtg acggccaatg gcgcgcgcag ctgacagcac gtactgcagg tcagcggagc
      241 catcctcatc cggaggagta cccacggcga tgaactggat ggtgccgaac tgcaccgcgt
      301 cctgaacatc tgtcgtgaaa tgcagccgtc cggcggcaac gttacgagcg acgacctgat
      361 caaggcccgg ctcgtgaatg ggaatgcccc cctccttcag cacccggatc ttctcgggat
      421 ccagatccag gcacaacacg tcgttgccca tctcggcaag acaggccccg gacaccagac
      481 ccacataacc cgtaccgacc accgtaattt tcatatgcga gttcccgcga gcaatcctga
      541 gagtgttttc aaaaccggtg ggcgtggccc aaaccggaaa gccatggtcg ccaagccagc
      601 ttagggcgtc agatcgaatt cttcggtgcg cttgggcgga taggtttccc accccccgca
      661 ggccggacaa cgccaataga actggcgtgc cttgaaacca caggcatcac agcggtaacg
      721 ggccaccctg cgggtgtgcc catgcaccaa ccccttgacc agttcgatat cggtacgttg
      781 ctcgatgggt gcggtcaata acgccgcctc gagaagcttg tcgagaccca ggagcgtcgg
      841 attgcgtcgc agttcgtcac gcaccagttg ataggctgcg gccggccctt ccttctgcag
      901 ctcccactga aaaagggcgt caagcaggtc gagggacgca tactgctcaa ggtaactgcg
      961 caacagctgc atgccctgct caagttcgcc gaggcgacga taggcatcca tcaagcgctc
     1021 cgcgaccagg gccagataaa ccgggttctg accttcaacc cgcttccagg cctctagcgc
     1081 cgcccgatca tccccctggg caacgtacag atcacccagc agcatgctgg cacgtacaca
     1141 tttccggttc gtctcaagcg cccgcagcaa agccccccgg gcttcgtcga agcgcgaatt
     1201 ggccatgtcg gcagaggcga gctcgcagtg aaaattggcc atttcctttt cccagagggc
     1261 gctctcgtgc cccgggagcg catcggccgt ttcaatggcc ttgcgccagt ccttttcctg
     1321 ctgatagata tcgaggaggt tcttgagtgc aaaatcgttg aggcggctgc cgcgcagctg
     1381 gacaaaaacg gcttcagcgc gatccagaag cccggccttg aggaaatcca gccccagttc
     1441 ggacagagcc tgcaatctct gatcgtccga aagatcatcc cgatcaatga gattctggtg
     1501 catgcggatt gcacgatccg tttcgcccct ccgccgaaag aggcttccca gggcgaagtg
     1561 caactcgaca gtctggttgt cgatcttcac cgcctcgatg aaggcgtcga tggccttgtc
     1621 gggctgctcg ttgaggagga agttcagacc ggtcaggtaa gtacgcggca aggcccgcga
     1681 ctccttgacc aggtgcttga tgtcaattcg ggcagccagc cagcccagca cgaaaaacaa
     1741 gggaaatgcc agcaaccacc atagctcgaa ttccatgggt tcagtaggtc tccggcagat
     1801 cgggaccatg ggcaatgggt gccttcccgc gcccttcccc aggggtagca gccccctgat
     1861 cgcgcagatg accgatctcg cgatgctggc ggtaaagcgt ggtcaaggta gcagtcaccc
     1921 caatcagcac accaatggtg acaaacagga ggatgaccag aaccagcggc acctgccatt
     1981 gggcaccgaa gaagaaccgc agaacgacaa catcatcatt ctttacggca aagccaaaaa
     2041 gcagcaggaa caggaagagt ctgaagaccc agatgagagc gcgcatgccc gaaattatcc
     2101 ccgacgtgga caaaaaaaag aaggcggctt tcgccgcctt caggatcgag ccttcggcct
     2161 ccggatgggg aagcgtcaac cagggtcacc ccatcatgtc gacacgttcc ctcaactcct
     2221 tgccagcctt gaaatgcgga acatacttct ccggcacttg caccttctcc cccgacttgg
     2281 gattgcgccc gacacgggga ggacggtaat tcaaggcaaa gctgccaaag ccgcggatct
     2341 cgatgcgatc accccgcg
//
LOCUS       DAORVN010000327         2358 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_45393_length_2358_cov_3.250109, whole genome shotgun sequence.
ACCESSION   DAORVN010000327 DAORVN010000000
VERSION     DAORVN010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2358
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45393_length_2358_cov_3.250109"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..971
                     /gene="priA"
                     /locus_tag="PKV23_07145"
     CDS             <1..971
                     /gene="priA"
                     /locus_tag="PKV23_07145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HQX85245.1"
                     /translation="APFIENITATLASGDQALLFLNRRGYAPVTVCRACGHRMDCPNC
                     AASLVEHRFRRQLICHHCGHHEPTPQSCPQCGVEGKLVPCGPGIERLAEEAVKRFPEA
                     RIAILSSDLTRGVFLRDVIRDVAKGEYNLVIGTQLVAKGHHFPFLTFVGVVDADLALE
                     SSDPRAGERTWALMAQVAGRAGRGEKPGHALVQTHMPEHPLMQALKKGDREGYLAQEK
                     LIRENAGLPPYGRLAAIIIAGSDAAETEQFVRLVARAAPTGEGVVILGPATAPVHIVR
                     GHFRWRFLVKAPREVNIQAFLRQWLKDIKPKGSIHLGIDVDPYSFL"
     gene            1052..1540
                     /locus_tag="PKV23_07150"
     CDS             1052..1540
                     /locus_tag="PKV23_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009759612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DM13 domain-containing protein"
                     /protein_id="HQX85246.1"
                     /translation="MSRKLIATAIIAGTIGFVAGNAFWYLASPLWIDRVVAEELPVEL
                     QMKTAAMGEFRDADSVHHGKGTATIFENDSGTKVLRFTNFEATNGPDLKVWLVKAENI
                     KASADVKASTWVGLGPLKGNIGDQNYVIPSDVNLSDYKSVVIWCEQFGVLFSAADLKP
                     GV"
     gene            complement(1537..>2358)
                     /locus_tag="PKV23_07155"
     CDS             complement(1537..>2358)
                     /locus_tag="PKV23_07155"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HQX85247.1"
                     /translation="GPGSDADALASDWFSGAVTGRYAGRITKARFMLDGEEVRLVPNV
                     GEDQLHGGPQDFCNDMWKSAYIDNGIRFTLHSPDGDQGFPGAMEVSATYRLIDTVLSL
                     DLEATTTKPTVVNLTNHAYWNLAGSGSGLDQEVEMPADHYLPLQGPKLPTGEIRDVTG
                     TVFDFRKLRKVGQAYDACFCLNGKRGQLRKGLTLRDPDSGRTMEVYTTECAIQFYTAD
                     HFGPKLPGKYGPLVQHGSIAIEPQNYPDAPNHANFPSSVLRPGEVYRHRIEWRFP"
BASE COUNT          479 a          746 c          658 g          475 t
ORIGIN      
        1 cggccccctt cattgaaaac atcaccgcca ctttggcgtc aggcgatcag gcccttctct
       61 ttctcaaccg ccgcggctat gcccccgtca ccgtctgccg cgcctgtggc catcgcatgg
      121 attgccccaa ttgcgccgcc tcgctggtgg agcaccgttt ccgccgccag ctcatctgcc
      181 atcactgcgg ccatcatgaa ccaacgccgc agtcctgccc gcaatgcggc gtcgaaggca
      241 aactcgtgcc ctgcggtccc ggcatcgagc gcttggccga agaggcagtc aagcggtttc
      301 ccgaagcgcg gattgccatt ctgtccagcg acctcactcg cggcgttttc ctgcgcgatg
      361 tcatccgcga cgtagccaag ggcgaataca atttggtgat tggcacccag cttgtggcca
      421 agggtcatca ctttccgttt ctcacctttg tcggagtcgt cgatgccgac ctcgccctgg
      481 aatcctccga cccgcgcgcc ggcgagcgca cttgggccct catggcgcaa gtggctggcc
      541 gcgccggccg cggtgaaaag cccggccatg ccctggtgca aacccacatg cccgaacacc
      601 ccctcatgca ggccctgaag aagggtgacc gtgagggcta tctcgcccag gagaaactca
      661 tccgcgagaa tgccggactc ccgccctacg gacggcttgc cgccatcatc atcgccggta
      721 gcgacgcggc ggaaactgag caattcgtgc gccttgtcgc cagggcggca ccaacgggcg
      781 aaggcgttgt gatcctggga ccggcaaccg cccccgtcca catcgtccgc gggcatttcc
      841 gctggcgctt tctggtaaag gccccgcgcg aagtgaatat ccaggctttc ctgcgccaat
      901 ggctgaagga cataaagcca aagggctcaa tccatctcgg gattgatgtg gacccctata
      961 gtttcttgta atcacgcctg gtaccgggaa tgtgagttgc agtgatcttt cacttcacgc
     1021 aatgaacggt tcagcaacca aggagcaaat catgtcccga aagctcatcg caacggccat
     1081 catcgccgga accatcggtt tcgttgcggg caatgctttt tggtatctcg cctcaccctt
     1141 gtggatcgac cgtgtggtgg ccgaagaact tcccgtggag ttgcaaatga aaactgcagc
     1201 catgggtgaa ttccgtgacg ccgacagcgt gcatcacggc aagggtacgg ccaccatttt
     1261 tgaaaacgac tccggcacaa aagtcctgcg ctttacaaat ttcgaggcca ccaacggccc
     1321 agacctcaaa gtctggctgg taaaagccga aaacatcaag gcctccgccg atgtaaaagc
     1381 cagcacatgg gttggcctcg gcccgctcaa gggtaatatt ggtgaccaga attatgtaat
     1441 cccaagtgac gtcaacctct cggactacaa gtcagtcgtc atctggtgtg agcaattcgg
     1501 cgtgctgttt tccgccgctg acctgaaacc tggcgtttaa ggaaaccgcc attcaatgcg
     1561 gtggcggtaa acttcgccgg ggcgaagaac ggaggaggga aagttcgcat ggttcggtgc
     1621 atcggggtag ttctgcggtt caatcgcaat agacccgtgc tgcaccagcg ggccatactt
     1681 gccgggaagc ttcggcccga aatggtccgc tgtgtaaaac tgaatggcgc actctgtcgt
     1741 gtaaacctcc attgtccggc ctgagtccgg atcgcgcaac gtgaggccct tacgcaattg
     1801 cccgcgcttt ccattcaggc agaaacaggc gtcataggcc tgtcccactt tgcgaagctt
     1861 gcggaaatca aacaccgttc ccgtcacatc cctgatttcg ccagtgggga gcttgggtcc
     1921 ctgcagcggc aggtaatgat cggcgggcat ctccacttcc tgatcaaggc cggaaccgct
     1981 acccgccagg ttccagtagg catggtttgt gaggttgacc accgtgggct ttgtcgtcgt
     2041 ggcttcaaga tcgagtgaca gcaccgtgtc gatcaaccgg taagtggcgc tcacctccat
     2101 ggcgccggga aacccctggt cgccatcggg cgaatgcaac gtgaagcgga tgccattgtc
     2161 gatataggca gacttccaca tgtcattgca gaaatcctgc gggcccccgt gaagctggtc
     2221 ttcccccaca ttgggaacaa ggcggacttc ctcgccatcc agcatgaaac gcgccttagt
     2281 aattcgcccg gcataccgcc ccgtgaccgc gcccgaaaac cagtcgctgg ccagggcgtc
     2341 cgcatcgctt cccggccc
//
LOCUS       DAORVN010000328         2357 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_45439_length_2357_cov_2.820156, whole genome shotgun sequence.
ACCESSION   DAORVN010000328 DAORVN010000000
VERSION     DAORVN010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2357
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45439_length_2357_cov_2.820156"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="PKV23_07160"
     CDS             <1..201
                     /locus_tag="PKV23_07160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85248.1"
                     /translation="IIAAYPLRGACKSVPMRIDRFDVWRMGSHAIAIEGGTAAITTAR
                     GSSGNRPWVVVPEPRRKLAPAR"
     gene            255..554
                     /locus_tag="PKV23_07165"
     CDS             255..554
                     /locus_tag="PKV23_07165"
                     /inference="COORDINATES: protein motif:HMM:NF013939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HQX85249.1"
                     /translation="MNSAAKKASIVNLRQEIDVVDERLIKLLAERQRLVEAIVIVKHA
                     EGIPARVPARVDYVIDRARVLARAHHLDPALAEAIWTEMVEWFVAHEERVLDMTK"
     gene            1038..>2357
                     /locus_tag="PKV23_07170"
     CDS             1038..>2357
                     /locus_tag="PKV23_07170"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015317521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HQX85250.1"
                     /translation="MDATVISKSRLPSRHVTVGPQRAPHRSYLYAMGLNEQQISQPLV
                     GVASCWNEAAPCNISLMRQAQSVKRGVAAASGTPREFCTITVTDGIAMGHQGMKASLV
                     SRECIADSVELTMRGHCYDALVGLAGCDKSLPGMMMAMVRLNVPSIFIYGGSILPGTF
                     KGRPVTVQDVFEAVGKHSVGDMSDADLHTLEQVACPSAGSCGAQFTANTMATVAEAIG
                     LALPYSCGAPAPYEIRDTFCFTSGEKVMELIARNIRPRDIVTLKALENAATVVAASGG
                     STNAALHLPAIAHECGIKFDLFDVAEIFKKTPYIADLKPGGKYVAKDMFEAGGIPLLM
                     KTLLDHGFLHGDCLTVTGRTIAENMASVKWNPDQDVVYPANKPITKTGGVVGLRGNLA
                     PEGAIVKVAGMKHLTFTGPARCFDSEELCFEAVTHKKYKEGEVLVIRY"
BASE COUNT          489 a          739 c          651 g          478 t
ORIGIN      
        1 atcattgccg cctatcccct gcgcggagcc tgcaagtccg tgccgatgcg catcgaccgc
       61 tttgatgtct ggcgcatggg ctcccacgca atcgccattg agggtggcac cgcggccatc
      121 acgaccgccc ggggctcgag tggaaacagg ccttgggtcg tcgtgcccga accgcgccgg
      181 aaattggctc cggcccgttg acgcggcgct tcgacacatt catgtatccc ccgatccgac
      241 cgaatgtgaa agccatgaat tcagcagcaa aaaaagcaag tattgtaaac ctccgccagg
      301 aaatcgacgt ggttgatgaa aggctcatca agctccttgc cgagcgccag cgcctggttg
      361 aagcgatcgt catcgtgaaa catgccgagg gcattccggc ccgcgttccc gcccgtgtcg
      421 attatgtcat tgaccgggcc cgggttctcg cccgcgcgca tcatcttgat cccgccctgg
      481 cggaagcaat ctggaccgaa atggtggaat ggttcgtggc ccatgaagag cgcgtcctgg
      541 acatgactaa atagctgctc aggcggtctc aagagacgtc gatcggcacc ctggcgttaa
      601 cccgtcggcg agtcgccgtt gatacgtctt caaggcaatt atgttccccc gtggttgcga
      661 gagaatttgc catagtggcc aatctgccac ggccgtgcac cgtcgaaaac agctgtttta
      721 gggccaaaaa taaagggtat taaccttttt gttgctgaaa tgccacagac ctccgaattg
      781 tgctgccgcc gcatttttgc tccacaatga acctttcatc cgcccaaatt gccccttagc
      841 gtgaacttat ccacgggagc tgagacctgt ggaatcagag cttacggggc ttctgggaaa
      901 tattgggcgg ggttgctctt gatcgccatg caacgggtga tcaaggagcg gggccgccgt
      961 ttgtttgaga aggaaggact gaaagctgtg ctgccgttaa caagaaaaaa gcaaaaacgc
     1021 tgacacagga aataattatg gacgccaccg tcatttcaaa atccaggctc ccgagccgcc
     1081 atgtaaccgt tggaccgcag cgtgccccgc accgctccta tctttatgcc atgggcctca
     1141 atgagcagca gatcagccag cccctcgtgg gcgtcgccag ctgctggaat gaagcggctc
     1201 cctgcaacat ttcgctgatg cgccaggccc agtcggtcaa gcgcggcgtc gccgctgcca
     1261 gcggcacccc ccgcgaattc tgcaccatca ccgtcaccga cggcatcgcc atgggccatc
     1321 agggcatgaa ggcctcgctg gtctcgcgcg aatgcatcgc cgactcggtc gaactcacca
     1381 tgcgcggcca ttgctatgac gcgctcgtgg gccttgccgg ttgcgacaag tcgctgcccg
     1441 gcatgatgat ggccatggtg cgcctcaatg tgccctccat attcatctat ggcggatcga
     1501 tcttgcccgg caccttcaag ggccgtcctg tcaccgtgca ggacgtgttt gaagccgttg
     1561 gcaagcacag tgtcggcgat atgtccgatg ccgacctcca caccctggaa caggtcgcat
     1621 gcccgtcagc gggctcctgc ggggcccagt tcaccgccaa caccatggca accgtggcgg
     1681 aagccatcgg ccttgccctg ccatattcct gcggagctcc agccccttac gaaatccgcg
     1741 atactttctg cttcacttcc ggtgaaaagg tgatggaact gatcgcccgc aatatccgtc
     1801 cgcgcgacat cgtcaccttg aaggcgcttg aaaatgccgc aacggttgtg gccgcctccg
     1861 gtggctccac caatgccgca cttcacctgc cggccattgc ccatgaatgc ggcatcaagt
     1921 ttgatctctt tgacgtcgct gaaatcttca agaagacgcc ctacatcgcc gatctcaagc
     1981 ccggcggcaa atatgtggcc aaggacatgt tcgaggccgg cggcattccg ctgctgatga
     2041 agaccctgct tgaccatggc ttcctccatg gtgattgcct gacggtcacc ggccgaacca
     2101 ttgccgaaaa catggcctcg gtgaaatgga acccggacca ggacgtggtc tatcccgcca
     2161 acaagcccat caccaaaacc ggtggtgttg tcggcctgcg cggcaatctc gctcccgagg
     2221 gcgccatcgt gaaggtggcc ggcatgaagc acctgacctt caccggcccg gcccgttgct
     2281 ttgactccga ggaactctgt tttgaagccg tcacccacaa gaaatacaag gaaggcgaag
     2341 ttctcgtcat ccgctac
//
LOCUS       DAORVN010000329         2353 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_45562_length_2353_cov_4.090513, whole genome shotgun sequence.
ACCESSION   DAORVN010000329 DAORVN010000000
VERSION     DAORVN010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2353
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45562_length_2353_cov_4.090513"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..603)
                     /locus_tag="PKV23_07175"
     CDS             complement(<1..603)
                     /locus_tag="PKV23_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HQX85251.1"
                     /translation="MAKEKFLRDKPHCNIGTIGHVDHGKTSLTAAITKVLAETGGATY
                     TAYDQIDKAPEERARGITISTAHVEYQTKNRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLARQVGVPALVVFMNKVDMVDDPELLDLVEMEVRELLTS
                     YQFPGDDIPIIRGSALAALENKTPEIGHDAILKLMAAVDSY"
     gene            complement(631..>2353)
                     /gene="fusA"
                     /locus_tag="PKV23_07180"
     CDS             complement(631..>2353)
                     /gene="fusA"
                     /locus_tag="PKV23_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012171081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HQX85252.1"
                     /translation="TVWRQGDKYQVPRIVFCNKMDKTGASFKQCIDDIKERLGARPVP
                     IQLPIGAEAEFKGIVDLVRMKAVVWENEALGANYHDEEIPADMLDAAKQARADMIEAC
                     AELDEQATEDYLNGKEISNETIKKLLRKAVLTAAFFPVLCGSAFKNKGVQPLLDAVVD
                     YLPSPLDRPAIKGIDVKTGGETTRPSSDKEPLALLAFKLMDDQYGILTFCRIYSGVLG
                     KGTSLLNSTRDKTERVGRMVLMHANNREEIAEAHAGDIVALVGLKDTRTGDTLCDPAK
                     PVILEKMEFPEPVIEMKVEPKTKVDQEKMAVALYKLAAEDPSFRVSTDSESGETIIKG
                     MGELHLDIKVDILKRTHKVEVSVGAPQVAYRETITRKTEVDYTHKKQTGGTGQFARVI
                     LEIEPNEKGKGYEFESQVVGGTVPKEYIPGVEKGLNSVMGSGILAGFPIVDIKIALTD
                     GAYHEVDSSAIAFEIAARMALREGLQKAGAILLEPIMKVEVTSPEDYLGGVIGDLNSR
                     RGQIMGTSARGNAQIVNAMVPLANMFGYINNLRSMSQGRASYTMQFDHYEQVPGAVAL
                     EVQKKYA"
BASE COUNT          447 a          684 c          685 g          537 t
ORIGIN      
        1 atagctgtca actgccgcca ttagcttcaa gatggcgtca tggccgatct ccggcgtctt
       61 gttctcaagc gccgccaggg ccgagccccg gatgatcgga atgtcgtcgc cggggaactg
      121 gtaggaggtg agcaactcgc gcacttccat ctcaaccagg tccaggagct ccggatcgtc
      181 aaccatgtcc accttgttca tgaacacgac cagggccgga acgccaacct ggcgggccag
      241 caggatgtgt tcccgcgtct gcggcatcgg gccgtcagcc gccgaaacaa caaggatggc
      301 gccgtccatc tgcgccgcac cggtgatcat gttcttcaca tagtccgcgt gtcccgggca
      361 atccacatgg gcgtaatgcc ggttcttcgt ctggtactcc acatgtgccg tggaaatcgt
      421 gatgccgcgg gcccgctctt cgggcgcctt gtcgatctgg tcataggccg tgtacgtcgc
      481 cccgcccgtc tccgccagga ccttggtgat cgctgccgtc agcgaggtct tgccgtggtc
      541 aacatggcca atcgtcccaa tgttgcaatg cggcttgtcg cgaagaaatt tctctttggc
      601 catcggagtg ctccgttaaa caaatttgga ttaagcgtat tttttctgga cttccagggc
      661 aactgcgccc gggacttgtt catagtggtc gaactgcatg gtgtagctgg cgcgtccctg
      721 gctcatcgag cgcaggttgt tgatgtagcc aaacatgttg gccaacggca ccatggcatt
      781 cacaatctgc gcgttgccgc gcgccgaagt gcccatgatc tggccacgac gggaattgag
      841 atcgccgata acaccaccca gataatcttc aggtgacgta acttcaacct tcatgatcgg
      901 ctcgaggagg attgcgccgg ccttttgcag gccttcacgg agcgccatgc gcgccgcaat
      961 ttcaaaagca atcgccgaag agtcaacttc atggtaggcg ccgtcggtga gcgcgatctt
     1021 gatgtcaacg atcggaaagc ccgcgagaat gcccgatccc atgaccgagt tcaggccctt
     1081 ttcaacgccg gggatatatt ccttcggaac cgtgccgccc acaacctggc tttcaaactc
     1141 gtagcctttg cccttttcat tgggctcgat ttcgagaatg acgcgggcaa actggcccgt
     1201 gccgcccgtc tgcttcttgt gggtatagtc gacttcagtc ttgcgggtga tggtttcgcg
     1261 gtaggcaacc tgcggcgcgc caactgacac ttcaactttg tgggtgcgct tcaggatatc
     1321 aaccttgata tccagatgaa gctcgcccat gcccttgatg atcgtctcgc cggactcgga
     1381 gtcggtggaa acacggaaag aaggatcttc ggcagccagc ttgtaaagcg ccaccgccat
     1441 tttttcctgg tcaaccttag tcttgggttc gaccttcatt tcaatgacgg gctcggggaa
     1501 ttccatcttt tcgaggataa ccggcttggc cgggtcgcac agcgtatcgc cggtgcgtgt
     1561 gtctttcagg ccgacgaggg ccacaatgtc gcccgcatgg gcttcggcaa tttcttcgcg
     1621 gttgttggca tgcatgagaa ccatgcggcc cacgcgctcg gtcttgtcgc gggtggaatt
     1681 gagcagtgag gtgccttttc caagcacgcc ggaatagatg cggcagaagg tgagaatgcc
     1741 gtactggtcg tccatcagct tgaacgcgag aagcgcaagc ggctccttgt cggatgacgg
     1801 acgggtggtt tcaccgccgg tcttcacgtc gatgcccttg atcgccggac ggtcaagcgg
     1861 cgaaggaagg tagtcaacaa ccgcgtcaag aagcggctga acgcctttgt tcttgaaggc
     1921 tgagccgcag agaaccggga agaaggcagc cgtcagaact gccttgcgaa gcagtttctt
     1981 gatggtctcg ttggaaatct ccttgccgtt caaatagtct tccgtcgcct gctcgtcgag
     2041 ttcggcgcag gcttcgatca tgtcggcgcg cgcctgcttg gcggcatcga gcatgtcggc
     2101 gggaatctct tcgtcatgat agtttgcccc gagagcttcg ttctcccaga ccacagcttt
     2161 catgcgtacg aggtcaacaa tgcccttgaa ttcggcttca gcgccaatcg gcagctggat
     2221 cggaaccggg cgtgcgccca ggcgctcctt gatgtcgtcg atgcactgct tgaaggaagc
     2281 accggtcttg tccatcttgt tgcagaacac gatgcgcgga acctggtact tgtcgccctg
     2341 gcgccagacc gtt
//
LOCUS       DAORVN010000330         2349 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_45724_length_2349_cov_2.817350, whole genome shotgun sequence.
ACCESSION   DAORVN010000330 DAORVN010000000
VERSION     DAORVN010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2349)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2349)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2349
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45724_length_2349_cov_2.817350"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1149)
                     /locus_tag="PKV23_07185"
     CDS             complement(<1..1149)
                     /locus_tag="PKV23_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HQX85253.1"
                     /translation="MLSPLKLLLVLISALGLAAGFLAPYFGLNALQATIWGLAAAFVL
                     LFLAREILTSLWRGEFGLDIVAALSMSGALYFGETLAAAVVSLMYSGGQLLENFAEAR
                     ARSEMNTLLGRVPTKALRYLDGNLSEVRIEELVPGDRILVRQGEIVPVDGKVASGRAL
                     LDQSILTGESVPVYRNVGDDVLSGSSSLDMAFDVIAIRPASQSTYAGIVRLVKAAQEA
                     KAPMVRLADRFAMWFLALTVLLAGGTWMITGEHLRMLAVLVSATPCPLILAVPVAIIS
                     GISKAARRGVLVKGGPVLETLARASVLVIDKTGTLTEGRARLVDVHSSGRRSADEILR
                     LAASLDQASQHVIAKSIVEAASERGLTLSMPVRAKEMAGSGIEGHVDGK"
     gene            1326..>2349
                     /locus_tag="PKV23_07190"
     CDS             1326..>2349
                     /locus_tag="PKV23_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011510987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQX85254.1"
                     /translation="MPKQYDQGGRATAGAFRRPSEGSSMPGARSLKQRVGALKNLRPF
                     MAMVWRASPGLTAASLLLRLIRALLPVATLYVGKLIIDEVVLLVQMPGKPGTLQQWWD
                     SGHLNWLGLLLAAEFALAVLADILGRIVSLIDSLLSERVTNSSSIRLMEHAATLDLED
                     FENAEFQDQLERARRQTSGRMTLMGQLLSQAQDLVTVTSFAAGLIFYAPWLIVLLLLA
                     LVPAFLGEAHFNAQSYSLDFGRTPERREMDYVRQTAASVETAKEVKIFGLNGFLIDRY
                     VKLATAFYAANRRLALRRAGWGGLFTAIGTVGYYLAYAYIAWRTLAGEFSVGDLTFLA
                     GSFRRLR"
BASE COUNT          453 a          718 c          718 g          460 t
ORIGIN      
        1 cttcccgtcg acatggcctt caatgcccga accagccatt tccttcgccc tgactggcat
       61 gctgagggtt agtccgcgtt cgctggctgc ctcaacgatt gacttcgcga tgacatgctg
      121 ggacgcctga tcaagcgagg cggcaaggcg cagaatctca tccgcactgc gccggccaga
      181 tgaatgcaca tcaacaagcc tggcgcgccc ttcggtgagc gtgccggttt tgtcgatgac
      241 cagcaccgag gcccgcgcca gagtttctag caccggcccc cccttgacca gcacgccgcg
      301 ccgcgcggcc ttgctgatcc cggaaatgat ggcaaccggg accgcgagaa tgagcgggca
      361 tggggtggcc gaaacaagca ccgcgagcat gcgcaggtgt tcgccggtta tcatccatgt
      421 cccgcctgcg agaagcacgg tcagagccag gaaccacatt gcaaaacggt cagcgaggcg
      481 caccatgggc gcctttgcct cttgcgctgc cttaacaagg cgcacaatgc cggcataggt
      541 gctctgagat gcggggcgga tggcaatgac gtcaaaggcc atgtcgaggc tggaggagcc
      601 gctcagcacg tcgtcaccga cattgcgata caccggaaca gattcgccgg tgaggatgga
      661 ttgatcgagc agggctcttc cgcttgccac cttgccgtcg acagggacga tctcaccctg
      721 gcgcacaaga atgcggtcac ccggtacaag ctcttcaatg cgcacctcgg acaagttgcc
      781 gtcgaggtag cgcagcgcct tcgtgggcac tcgcccgagg agcgtgttca tttccgagcg
      841 ggcccgcgcc tcggcaaaat tctccagcaa ctggccgccg gaatacatga gggagacaac
      901 ggccgcagca agcgtctcgc cgaaataaag agctcctgac attgacaggg ccgcaacgat
      961 gtcgaggcca aactcgccgc gccacagcga tgtaagaatc tcccgggcga ggaacaggag
     1021 gacaaatgcg gccgcaagtc cccatattgt ggcctgcagg gcattcaaac cgaaataagg
     1081 tgccaggaac cccgctgcca gcccaagggc cgatatcagg accagcagca atttgagtgg
     1141 gctaagcacg gcgtgccttt caatcttggg tattcctgtc gagcgactct tgaaactgaa
     1201 tttgcagcgc cgggactgcc cttgcaagga ctgcctctgg tatttgactt gggaacgttg
     1261 ctcattcacc atggaattgt agaggatagc tccttacggt ttccgccagg caaaggagtg
     1321 gtttcatgcc gaaacagtat gaccagggcg gaagggcaac ggctggggcg ttccggcggc
     1381 cctctgaagg gtcttccatg ccgggcgcca gatccctcaa gcaacgggtc ggcgctctca
     1441 agaacctgcg ccccttcatg gcgatggtgt ggcgtgccag tccaggcttg actgctgctt
     1501 cgctcctgct gcgcttgatc cgggctcttc ttccagttgc gacactatat gtcggcaaac
     1561 tgatcatcga cgaagtggtg ctgctggtcc agatgccggg caagccaggg acgctgcagc
     1621 agtggtggga cagcgggcat ctcaactggc tcggcctctt gctcgcggcc gaattcgcgc
     1681 tggcggttct ggccgatatt ctcgggcgca tcgtatcgct gatcgacagc ctgctttcgg
     1741 aacgggtgac gaattcctca agcatccgcc tgatggaaca tgccgcgacc cttgatctcg
     1801 aagatttcga aaacgccgag ttccaggacc agctcgagcg ggcgcgccgc cagaccagcg
     1861 ggcgaatgac gctgatgggc cagctcctga gccaggcgca ggatcttgtg acggtcacca
     1921 gctttgccgc gggcctcatc ttttatgcgc cgtggctcat tgtcttgctg cttctcgccc
     1981 tggtgccggc tttccttggg gaggcgcatt tcaacgccca gagctattcg ctcgatttcg
     2041 gccgcacgcc ggagcggcgg gaaatggatt atgtcaggca gacggcggca agcgtggaaa
     2101 ccgccaagga agtgaagatc ttcggcctca acggtttcct gatcgaccgc tatgtgaaac
     2161 tcgccaccgc cttctatgcg gccaatcgcc ggctggcgtt gcggcgcgcc ggctggggcg
     2221 ggttgttcac ggccatcggc accgtgggtt attaccttgc ctatgcctat atcgcctggc
     2281 gcacgctggc tggcgagttc tcggtcggag atctcacttt tctcgccgga tccttccgcc
     2341 gtttgcgca
//
LOCUS       DAORVN010000331         2339 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_46077_length_2339_cov_3.983800, whole genome shotgun sequence.
ACCESSION   DAORVN010000331 DAORVN010000000
VERSION     DAORVN010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2339)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2339)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2339
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46077_length_2339_cov_3.983800"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /locus_tag="PKV23_07195"
     CDS             complement(<1..378)
                     /locus_tag="PKV23_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018320479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlpA-like double-psi beta-barrel
                     domain-containing protein"
                     /protein_id="HQX85255.1"
                     /translation="MRKLLSVTCILLAMSVTISGCSSNKSASRDPFAGIGSPRYKGDG
                     PVPMGGGRYHVGEPYQVAGRWFKPKEQPHYDKTGPASWYGEAFNRRMTSNGEWFDMTR
                     LTAAHPTLPLPSYAKVTNVNNGKT"
     gene            complement(495..1238)
                     /locus_tag="PKV23_07200"
     CDS             complement(495..1238)
                     /locus_tag="PKV23_07200"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQX85256.1"
                     /translation="MNRVLVSSLLVFMLGSASFAAAQTLEELPFSKKLDLAQVGDEEA
                     QMAVASTFENGSKETKKNIVEAARWYREAALKGNIEAEYRLARLVSKGAKGLTKDLPT
                     AAKLYEAAAGAGHLESMNAIGHAYQNGLGVPVDAAKAVEWYRKAAEGKLAVAQNNLGI
                     LYLNGKGVERNVSEAFKLFESAAKQGDGWGLNNLGGMYEMGWGVAKDRSKAATYYKQA
                     VEKGIEPAQANLARLMPATPPPVAPVTLN"
     gene            complement(1302..1730)
                     /locus_tag="PKV23_07205"
     CDS             complement(1302..1730)
                     /locus_tag="PKV23_07205"
                     /inference="COORDINATES: protein motif:HMM:NF021438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HQX85257.1"
                     /translation="MNLLRKLGIAGFFSLALPGLGLAGEGANGVWMLDSGKVTVRIMP
                     CGASLCGAIVGLAKPLTKDGQPKVDRKNPNEALRNRPLMGLKILANMKPAGENRWSGT
                     IYNADDGRTYSSYMNLSGSNMKVKGCVGPFCKTMVFVRRN"
     gene            1842..>2339
                     /locus_tag="PKV23_07210"
     CDS             1842..>2339
                     /locus_tag="PKV23_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006338612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HQX85258.1"
                     /translation="MSNVTNLSRHRSHDSNEWQLRVDLAAAFRLAARNDWHEAVANHF
                     SQAVSNDGKKFLMNPRWRHFSKVKASELLLLDTDDHATMEKPNAPDLTAWSLHGRLHA
                     LLPQARCIMHLHPPYATAIASLADPDVKPIDQNTARFYNRVAFDMDYGGMANTDEEGE
                     RIARLM"
BASE COUNT          455 a          734 c          635 g          515 t
ORIGIN      
        1 cgttttgcca ttgttcacat tggtgacctt ggcgtagctc ggcaggggaa gcgttggatg
       61 cgctgccgtg aggcgcgtca tgtcgaacca ttcgccattt gatgtcatgc gccggttgaa
      121 cgcctcgccg taccaggagg cagggcctgt cttgtcgtaa tggggctgtt ccttgggctt
      181 gaaccagcgg ccggcgacct ggtagggctc gcccacatga tagcggccac cgcccatggg
      241 gaccgggcca tcacccttgt aacgcgggct gccgatgccg gcaaacgggt cccgtgaggc
      301 ggatttgttt gatgagcagc cggaaatggt gacgctcatg gccaacagga tgcacgtaac
      361 actgagcaac ttacgcatac agactccacc aggcccccac ggcccctttg cgttgccgca
      421 aagccacaga gtcaactttc gcgccaagtg cgtaaaattt gagttgaatt gtttggttaa
      481 ctaattgcta accgtcaatt taaggtaacc ggcgcaaccg gcggcggagt tgccggcatg
      541 agccgcgcca gatttgcctg ggccggttct atgccctttt ccaccgcctg cttgtaatag
      601 gtcgcggcct tgctgcggtc cttggcgaca ccccagccca tttcatacat gccgccgaga
      661 ttgttcaggc cccagccatc accctgcttg gcagcggatt cgaacagctt gaaagcctcg
      721 ctgacgtttc gttcaacacc cttgccgttc aggtacagga taccgaggtt gttctgcgca
      781 acggcaagct tcccctcggc ggctttacgg taccattcga ccgctttggc tgcatccacc
      841 gggacgccca ggccattctg gtaggcgtgg ccaatggcgt tcatcgattc caggtgcccg
      901 gcgccggctg ccgcttcgta aagcttggcc gctgtcggca ggtccttggt caggcccttt
      961 gcccccttgc tgaccagccg ggcgagacgg tattccgctt cgatattgcc tttcagcgct
     1021 gcttcccgat accacctggc agcctcgacg atgttcttct tcgtttcctt gctgccattt
     1081 tcaaaggtgc tggccaccgc catttgcgcc tcttcgtcgc cgacttgggc gagatcgagt
     1141 ttttttgaaa aggggagctc ttcgagcgtt tgggcggcgg caaacgaggc gctgccaagc
     1201 atgaagacga gaagcgaact gacaagaaca cgattcatgg gactgcctta cccgggaaaa
     1261 acccatattg aaatacaatc atgacaacaa tctggcgctg ttcaattccg gcggacaaag
     1321 accatggttt tacagaaggg cccgacacag cccttcactt tcatgttgct tcccgagagg
     1381 ttcatgtagg aagaataagt tctgccatcg tcggcgttgt agatggtccc gctccagcga
     1441 ttctctcccg ctggcttcat gtttgccagg atcttgagcc ccatcagcgg ccggttgcgc
     1501 agcgcctcgt tggggttttt ccggtcaact ttgggctgcc cgtccttggt gagaggcttc
     1561 gccaggccaa cgatcgcccc gcaaagactg gctccgcatg gcatgattcg cactgtgacc
     1621 tttccgctgt ccagcatcca gaccccgttg gccccctcac ctgccagccc aagtcccggc
     1681 agcgccaggg agaaaaaacc tgcgattccc agtttcctca gcaaattcaa cgtcggcctc
     1741 catcatcacg ttccatcggc atccctgctt ccaaggcaat tgtacggcct tcataactca
     1801 gctagtttcc cgcttcccgg atatagcaaa ggaagcaggc catgagcaac gtcaccaacc
     1861 tcagccgcca tcgcagccat gactcaaacg agtggcaatt gcgtgttgac cttgcggcgg
     1921 cttttaggct ggcggcgcgc aatgactggc acgaggccgt ggccaatcat ttcagccagg
     1981 ccgtttcaaa cgacggcaag aagttcctga tgaatccgcg ttggcgtcat ttctcaaagg
     2041 tgaaagcctc cgagctcctg ctcctcgata ccgacgatca cgccaccatg gaaaagccaa
     2101 acgcgccaga ccttaccgcc tggtcgctcc acggcaggct tcacgccctg ctgccccagg
     2161 cccgctgcat catgcatctg cacccgccct atgccacggc cattgcctcg ctcgccgatc
     2221 ccgacgtgaa gcccatcgac caaaatacgg cgcgctttta taaccgggtg gcctttgaca
     2281 tggattatgg cggcatggcc aacaccgatg aggaaggcga gcggattgcc cgcctcatg
//
LOCUS       DAORVN010000332         2338 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_46122_length_2338_cov_3.343408, whole genome shotgun sequence.
ACCESSION   DAORVN010000332 DAORVN010000000
VERSION     DAORVN010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2338
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46122_length_2338_cov_3.343408"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(116..883)
                     /locus_tag="PKV23_07215"
     CDS             complement(116..883)
                     /locus_tag="PKV23_07215"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012111624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019674 - NAD metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HQX85259.1"
                     /translation="MNYSRIAFSASDAPEARKALSRLAKRYGNATPETADVVVALGGD
                     GHMLQTLHRYVSTGTPIYGMNRGSVGFLMNDYTEGQLLERLHAAELTTIHPLRMVATD
                     ESGKSHKGIAFNEVSLLRQRYQAAKLQISIDNKVRLEELICDGLLLSTPAGSTAYNLS
                     AHGPILPVNAPLLALTPISAFRPRRWRGAVLPHNAKVTITVLEPDKRPVASVADHVEV
                     RHVKAVEIAEDKKRSVKILFDPGHSLAERVLNEQFKF"
     gene            973..1347
                     /locus_tag="PKV23_07220"
     CDS             973..1347
                     /locus_tag="PKV23_07220"
                     /inference="COORDINATES: protein motif:HMM:NF019460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1636 domain-containing protein"
                     /protein_id="HQX85260.1"
                     /translation="MRSLVFCTTCRYSQNEASGTDGRTGGQTLIDHMKDLIAEKGRDD
                     IKIETQACLWNCKRHCSVVLRDDQRFSYFTGDHVPERGQAEAILAWFDLHGATLTGEV
                     PFREWPQAMRGHFIARIPRSRS"
     gene            1344..1901
                     /gene="cobU"
                     /locus_tag="PKV23_07225"
     CDS             1344..1901
                     /gene="cobU"
                     /locus_tag="PKV23_07225"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018387243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HQX85261.1"
                     /translation="MSTLLVLGGARSGKSRYAESLGKGQKFYIATAEASDEEMVQRIA
                     DHRLQRGPGWETFEVPLDLVTALQTIDGNGRFILIDCLTIWLSNLMIVKLDVTRETDM
                     LCETLRTMKAEVVLVSNEVGLGIVPANAVARTFRDAQGFLNQRVAAVADDVVFMAAGL
                     PLTLKKAVPKTPPKKAKKSSRSRKA"
     gene            complement(1834..>2338)
                     /locus_tag="PKV23_07230"
     CDS             complement(1834..>2338)
                     /locus_tag="PKV23_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012043270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015420 - ABC-type vitamin B12 transporter
                     activity [Evidence IEA];
                     GO_function: GO:0048472 - threonine-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CobD/CbiB family cobalamin biosynthesis protein"
                     /protein_id="HQX85262.1"
                     /translation="ENASDGIVAPVLWYALLGLPGIVAYKAINTADSMIGHKSERYIY
                     FGWAAARLDDLINLPASRLTGFLFAAAAAWNDQERGKIALQAMWREAPKHQSPNAGWP
                     ESALAASLGVKFGGPRSYEGALVDLPWMGEGRETLNRDDIRKGLRLYGTAMTFLLFLA
                     VFLALLF"
BASE COUNT          492 a          638 c          762 g          446 t
ORIGIN      
        1 atgaaaacat gcagagcgcc gagcgcagca gaaatctgga attcatcggg cgcttcgctg
       61 aagaccgggt gcgcaagatc gccaagcgcc tgcgcgccga catggtgcgc gccgcctaga
      121 acttgaactg ttcgttgagc acgcgctcgg ccaggctgtg gcccgggtcg aaaaggattt
      181 tcaccgagcg ctttttgtcc tcggcaatct cgacggcctt cacatggcgc acttcaacat
      241 ggtcggccac ggaagccacc gggcgcttgt cgggctccaa tacagtaatt gtcaccttgg
      301 cattgtgagg caaaacggcg ccgcgccagc gccggggccg gaaggcgctg atgggtgtga
      361 gggcgagtag gggggcattc actggcagga ttggtccgtg ggcggacaga ttataggccg
      421 tgctgccggc tggagttgac agaaggaggc catcgcaaat caattcctcg aggcggacct
      481 tgttgtcgat ggagatctgg agtttggcgg cctggtagcg ctgacgcaga agcgacactt
      541 cattgaaggc gatgcccttg tgggattttc cgctttcatc ggtggccacc atgcgcaggg
      601 ggtgaatggt ggtgagttcg gcggcatgca agcgctccag gagctgaccc tcggtgtagt
      661 cgttcatcag aaagccaacc gagccgcgat tcatgccgta gatgggggta ccggttgaaa
      721 cgtagcggtg cagggtttgc agcatgtggc catcaccgcc cagggccaca accacatcgg
      781 ccgtttctgg cgtggcattg ccatagcgct tggccaaccg ggacagggcc ttgcgggctt
      841 caggcgcgtc ggaagcggaa aaggcgatgc gggagtaatt catgtcgctt tcgtttgcgt
      901 tgacagatca aggctttgcc accattgaag gcgcgacgac catagcaatc accgaaggac
      961 cgaagcaagc ccatgcgttc gctcgtattt tgcacgactt gccgctattc ccagaacgaa
     1021 gccagtggca ccgatgggcg cacgggcggg cagacgctga ttgaccacat gaaggatttg
     1081 attgccgaga agggccgcga tgacatcaaa atcgaaacgc aggcctgcct gtggaattgc
     1141 aaacgccatt gcagcgtggt gttgcgcgac gaccagcgct tcagctattt taccggcgac
     1201 catgttccgg aacgcggaca ggcggaagca attcttgcct ggttcgacct gcatggtgcc
     1261 acgctgacgg gtgaagttcc gttccgcgag tggccgcaag ccatgcgcgg ccatttcatt
     1321 gcgcgcattc caaggagcag atcatgagca cgcttctggt tctcggcggg gcccggtcag
     1381 gcaagagccg ctatgcggaa agcttgggca aggggcagaa attctacata gcgacagcgg
     1441 aagcctcgga tgaggaaatg gtgcagcgga tcgccgacca ccggctccag aggggcccgg
     1501 gatgggaaac cttcgaagtg ccgcttgatc tggtaaccgc gctgcaaaca atcgacggca
     1561 acggtcggtt catcctgatt gattgcctga cgatctggct cagcaacctg atgatcgtaa
     1621 aacttgatgt cacacgcgag acggacatgc tgtgtgagac cctgcggacg atgaaggctg
     1681 aggtggtgct ggtgtcgaat gaggtgggcc ttggaattgt tccggccaat gcggtggccc
     1741 gaaccttccg tgacgcgcaa gggttcctca atcagcgagt ggcggcagtg gctgacgatg
     1801 tggtattcat ggcggcgggc ctgccgctca cgctcaaaaa agcagtgcca aaaacaccgc
     1861 caaaaaaagc aaaaaagtca tcgcggtccc gtaaagcctg aggcctttgc gaatatcgtc
     1921 gcggttcaag gtttcgcggc cttcacccat ccagggcagg tcaacaaggg cgccttcata
     1981 gctgcgcggc ccgccgaatt taacgccaag gcttgcggca agggccgatt caggccagcc
     2041 cgcattgggt gattgatgct tgggggcctc gcgccacata gcctgcaggg cgatcttgcc
     2101 gcgctcctgg tcattccagg cggccgcggc ggcaaacaga aaaccggtga ggcgcgaagc
     2161 tggcaggttg atcagatcat cgagccgtgc tgccgcccag ccgaaataga tgtagcgttc
     2221 ggacttgtgg ccgatcattg agtcagcggt gttgatcgcc ttgtaggcca cgatgccggg
     2281 caggccgagc agggcgtacc acagaacagg agccacaatg ccgtcgctgg cgttttcg
//
LOCUS       DAORVN010000333         2323 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_46675_length_2323_cov_4.145944, whole genome shotgun sequence.
ACCESSION   DAORVN010000333 DAORVN010000000
VERSION     DAORVN010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2323
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46675_length_2323_cov_4.145944"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            15..788
                     /locus_tag="PKV23_07235"
     CDS             15..788
                     /locus_tag="PKV23_07235"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008193895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQX85263.1"
                     /translation="MELKNRIAIITGAARGIGLSCAERFVAEGARVVVTDVLDDIGKA
                     EAKRLGATYLHCDVSSSSDVRATVATVVKQFGGVDILLNNAGISIPGDFLEMSEADYD
                     RVIDINLKGSFLMLQACAREMVKQVAAGKPPGAIINMSSVNDTLAIPTIVSYCIAKGG
                     VSQLTRATSISLAPHGIRVNAIGPGSIMTGMLHSVVSDKAAMARVMSRTPMGRVGEPS
                     EIAAIAVFLASDDSSYVTGQTIYADGGRMPLNYTVPVKE"
     gene            complement(850..1449)
                     /locus_tag="PKV23_07240"
     CDS             complement(850..1449)
                     /locus_tag="PKV23_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006934070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="short chain dehydrogenase"
                     /protein_id="HQX85264.1"
                     /translation="MRILAVGTAGDVGKAAVAELRPRHEIITAGRSSGDVRVDLLDAS
                     SISAMFEKLGKLDAVVACAGHAHYGPLAGMTETQFMKGLKDKLMGQVNLVLLGTPYVN
                     DRGSFTLTSGVLDRDPILKGVNGATVNAALKGFVTAAAIELERGIRINVVSPGLLEDS
                     EKKYAGLFPGQEMVSSKRVGLAYAKSVEGVLTGQVIIIA"
     gene            complement(1449..2207)
                     /locus_tag="PKV23_07245"
     CDS             complement(1449..2207)
                     /locus_tag="PKV23_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009798951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1013 domain-containing protein"
                     /protein_id="HQX85265.1"
                     /translation="MSNAPLMPKATAVWLVENTALTFRQIADYCNLHELEVKGIADGD
                     VATGIKGLDPVTGGQLTRDEIEKGEADPTYALKILVSKVNIPVQKSTGGPRYTPVSRR
                     GERPDAIMWLLRNHPELPESTIMKLVGTTKTTIAAIRDRTHWNASNIKPVDPVSLGLC
                     SQLELDFAVQKAARKAGKGQVPDAAPVRTLIPAAEVVGTVANVAAVAAAAAEAERAVT
                     AESAKNDGKQYSADSVFAKLKGLKLEPTEETTKE"
BASE COUNT          438 a          754 c          656 g          475 t
ORIGIN      
        1 attaggagac acgcgtggaa ctcaagaacc gaatcgccat catcactggc gcggcccgtg
       61 gcattggcct gagctgcgcc gaacgcttcg ttgccgaagg cgcccgcgtc gtcgtcaccg
      121 atgtgcttga cgacattggc aaggccgaag cgaagcggct tggcgccacc tatctccact
      181 gcgacgtttc aagttccagc gacgtcagag ccaccgttgc caccgtcgtg aagcagttcg
      241 gcggcgttga catcctcctg aacaatgccg gcatcagcat ccctggcgat ttcctcgaaa
      301 tgagcgaggc cgattacgac cgggtcatcg atatcaacct caagggttca ttcctcatgc
      361 tgcaggcctg cgcccgcgaa atggtgaaac aggtggcggc cggaaagcct ccgggggcca
      421 tcatcaacat gtcgtcggtc aatgacacgc tcgccattcc gaccatcgtc agctactgca
      481 tcgccaaggg tggcgtctcc cagctcaccc gcgccacctc catttccctg gccccccatg
      541 gcatccgcgt gaatgccatc ggccccggtt ccatcatgac cggcatgctc cacagtgtag
      601 taagtgataa agccgccatg gcgcgcgtca tgtcgcgcac tcccatgggc cgcgtcggcg
      661 agccctcgga aatcgccgcc attgccgtgt tcctggcatc cgacgactca agctacgtca
      721 ccggccaaac catctacgcc gacggcggcc gcatgccgct gaactacacc gtgcctgtga
      781 aggaatgagg cgataacaat ccaattctca tcatacacgc gtcaagcgcg ggtatgatga
      841 gaagtatgac taggcgatga taatgacctg ccccgtcaat acgccctcga cgctttttgc
      901 gtaagcaagg cccaccctct tcgatgacac catttcctgg ccagggaaaa gccccgcata
      961 tttcttctct gagtcttcca gcagtccggg gctcaccacg ttgatgcgga tgccgcgctc
     1021 cagttcgatg gcagcggcgg tgacaaagcc cttcaacgcc gcgttcactg tggcgccatt
     1081 aacccctttc aggataggat cacggtcaag cacgccgctg gtcagcgtga aagacccgcg
     1141 gtcattgacg taaggcgtgc ccagcaggac caggttcacc tgccccatca gcttgtcctt
     1201 caatcccttc atgaactgcg tttcggtcat gccggccagt gggccgtaat gggcatggcc
     1261 cgcacaggcc acaaccgcat ccagcttgcc gagcttttca aacatggcgc tgatggacga
     1321 ggcatcgagg agatcaacgc gcacatcgcc ggaagagcgt ccggcggtga tgatctcatg
     1381 gcgcggcctg agttcggcca ccgccgcctt gcccacatcg cccgccgttc cgactgcaag
     1441 gatgcgcatt attcttttgt ggtttcttcg gtgggctcaa gcttcaggcc cttcagcttc
     1501 gcaaagaccg agtcggcgct gtactgcttg ccgtcattct tcgccgattc cgccgtcacg
     1561 gctctttcgg cttctgccgc ggcagcggca acggccgcca cgttggcaac ggtgccgaca
     1621 acttcagccg ccggaataag cgtgcgcacc ggcgccgcat cgggcacctg gcccttgccc
     1681 gccttgcgcg ctgccttctg caccgcgaag tccagttcca attgcgagca caagcccagc
     1741 gaaaccggat cgaccggctt gatgttgctg gcgttccaat gggtgcggtc gcggatggcg
     1801 gcaattgtcg tcttcgtcgt gcccaccagc ttcatgatgg ttgattccgg cagttccgga
     1861 tggttgcgca gcagccacat gatggcatcg ggacgctcgc cccggcgcga caccggcgta
     1921 tagcgcgggc cgcccgttga tttctgtacc ggaatgttga ccttcgacac cagaatcttg
     1981 agcgcataag tggggtcggc ctcgcccttc tcgatctcgt cgcgggtgag ctggccgccg
     2041 gtcaccgggt caaggccctt gatgcccgtg gccacgtcgc catcggcgat gcccttcact
     2101 tcaagctcgt gcagattgca gtaatcggca atctggcgga acgtcagcgc ggtgttttca
     2161 accagccaga cggcggttgc tttcggcatc agcggggcat tggacataat tttttcgctc
     2221 cttcggaacc gtctggggtt ccgtaacatc agggtaacta cgcttgggaa tgccagctat
     2281 ataggcgctg cctcccgaag atgcaacgcg ttttaaacgg tca
//
LOCUS       DAORVN010000334         2323 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_46684_length_2323_cov_3.319665, whole genome shotgun sequence.
ACCESSION   DAORVN010000334 DAORVN010000000
VERSION     DAORVN010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2323
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46684_length_2323_cov_3.319665"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..485)
                     /gene="lptE"
                     /locus_tag="PKV23_07250"
     CDS             complement(<1..485)
                     /gene="lptE"
                     /locus_tag="PKV23_07250"
                     /inference="COORDINATES: protein motif:HMM:NF016290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HQX85266.1"
                     /translation="MKKVIYCLVVLLSLTGCGYKPLYSSGSDGRGVVASLSSIAIQET
                     HSRAGQLVRNSLLSSMRPASAAGDDLYKLILHPELAQSTLIDRSTPGIKRIRLRLTVT
                     YQLIETASGAEVNSGKTFSAVSYDIVREPVADLQAEANATSRAAKEVASDIHTRLAAF
                     MA"
     gene            complement(496..>2323)
                     /gene="leuS"
                     /locus_tag="PKV23_07255"
     CDS             complement(496..>2323)
                     /gene="leuS"
                     /locus_tag="PKV23_07255"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020187264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HQX85267.1"
                     /translation="IFGASFCAISADHELAKRYSVKDAGLAEFRREVSALGTSEETIE
                     RAEKRGYALGVYARHPFLPKLKLPVYAANFVLMGYGEGAIFGCPAHDQRDLDFARKYG
                     LPVIPVVAPRGVDPASVVIGTEAYLEKDGAESQMINSGFLNGLSVPAAKEEVARRFES
                     SGAGERKVNFRLRDWGVSRQRYWGCPVPVIHCPTCKIVPVPKTELPVTLPEDVTFDKP
                     GNPLAHHPTWKHVKCPKCGGGATRETDTFDTFIDSSWYYARFCSPKADVPVEAEAARY
                     WMPVDQYIGGVEHAILHLLYSRFFARAMGKTHHLDWDEPFASLFTQGMVVHETFKTEG
                     GDWVFPVDAVLRDGKWFHAKTGEELTIGGIEKMSKSKRNVVDPESIIESYGADTARWF
                     MLSDSPPERDIEWTEAGAEGAWKFTQRLWRIVGDASARKATAYTGEFGPAALELRRIA
                     HRTLAAVTDDLTSLRFNRAIARIYELSNALSTALATPGPAPDLSFAIREAADFLILMF
                     APMMPHLAEECWAAMNHKQPVVETSWPKPISALIVDDEVTIAVQVNGKRRDEIKLQKG
                     MARDLVEAAVMKLDGVKRALEGKPVVKFIVVPDRIVNIVVNG"
BASE COUNT          495 a          665 c          681 g          482 t
ORIGIN      
        1 gccatgaagg ccgcaaggcg cgtgtgaata tccgaggcaa cttccttggc ggcccggctc
       61 gtggcattcg cttcagcctg caggtcggca acaggttcgc gaacaatgtc gtaggatacg
      121 gcggaaaatg tctttccgga gttaacttcg gcacccgaag ccgtctcgat caactgatag
      181 gtgacggtga ggcgcagtct gatgcgcttg atgccgggcg tggaccggtc aatcaatgtg
      241 gactgggcca gttcgggatg cagaatgagc ttgtagagat catcgccagc ggcactggct
      301 ggccgcatgg atgacagcag gctgtttctt accagctggc ctgccctgct gtgggtttcc
      361 tgaatcgcaa ttgaagacag cgatgcaaca acgccgcggc catcgctgcc agacgaataa
      421 agcggcttgt agccacagcc ggtgaggctg agcagaacga ccaggcaata gatgactttt
      481 ttcacccgca aactcttatc cgtttacgac gatattcaca atacggtccg gaacaactat
      541 gaatttcacc acaggcttgc cttccagggc gcgtttgacc ccatcgagct tcatgacagc
      601 ggcctcgacg agatccctgg ccatgccttt ttgcagcttt atctcgtcac ggcgcttgcc
      661 attgacctgc accgcaatag tgacttcatc atccacaata agggctgaaa ttggtttggg
      721 ccaggacgtt tcgacaacgg gttgcttatg gttcatcgct gcccagcatt cctcggccaa
      781 atgcggcatc atgggcgcaa acatcaaaat caagaaatcg gcggcttcgc ggatggcgaa
      841 ggacaaatcg ggagcaggac caggggttgc cagggccgtg ctcaaggcgt ttgaaagctc
      901 atagattcgc gcgatcgccc gattgaagcg gagcgatgtc agatcatcgg tcacggcggc
      961 aagggtacgg tgggcaatgc gccgcaattc caaggcggcc gggccaaatt cgccggtgta
     1021 tgcggttgcc ttgcgggcac tcgcgtcccc cacgatgcgc cagagcctct gggtgaattt
     1081 ccaggcgcct tcggcgccgg cctcggtcca ttcaatatcg cgctcaggcg gcgaatcaga
     1141 cagcatgaac cagcgtgccg tatcggcccc atagctttcg ataattgatt cggggtcaac
     1201 cacattgcgt ttggatttcg acatcttctc gatgccgccg atggtcagtt cctctcccgt
     1261 cttggcatga aaccatttgc catcgcgcaa taccgcatcg accgggaata cccaatcacc
     1321 gccttcggtc ttgaaagttt catgaaccac catgccttgg gtgaacaggc tggcaaatgg
     1381 ctcatcccaa tcgagatgat gggtcttgcc catggcgcgg gcaaaaaagc gcgaatagag
     1441 aagatgcaaa atggcatgtt cgacaccacc gatgtattgg tccaccggca tccaatagcg
     1501 cgcggcttcg gcttccacgg ggacatccgc cttcggtgag cagaagcggg cgtagtacca
     1561 ggacgagtcg atgaaggtat cgaaggtgtc agtttcgcgc gtggcgccac ctccgcactt
     1621 cgggcacttt acgtgcttcc aggtcgggtg atgggccagc ggattgccgg gtttgtcgaa
     1681 ggtgacgtct tccggcaagg tcaccgggag ctcggtcttg ggcacgggaa caatcttgca
     1741 agtagggcaa tgtatgaccg gaaccggaca gccccagtat ctttgccgcg aaacgcccca
     1801 atcgcgcagg cgaaaattca ctttcctttc gcctgcccct gaagactcaa aacgccgtgc
     1861 aacctcttcc ttggcggcag gcaccgacag gccgttgagg aagcccgagt tgatcatctg
     1921 gctttcggct ccatccttct ccagataggc ttccgtgccg atgacaaccg atgccggatc
     1981 gacgcccctt ggtgcgacca ccggtatgac cggcaggcca tacttccggg caaagtccag
     2041 gtcgcgctgg tcatgggcgg ggcagccaaa aatagcgcct tcgccatagc ccatgaggac
     2101 gaagttggcg gcatagaccg gcagtttcag cttgggcaga aagggatggc gggcatagac
     2161 gcccagtgcg taacctcttt tctcggcacg ctcgatggtt tcctcgctgg tgccgagggc
     2221 ggatacctcg cggcggaact ccgcgagtcc agcgtccttg acggaatatc tctttgccag
     2281 ttcatgatcg gcggaaatcg cgcagaagct ggcgccgaaa atc
//
LOCUS       DAORVN010000335         2321 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_46754_length_2321_cov_4.122683, whole genome shotgun sequence.
ACCESSION   DAORVN010000335 DAORVN010000000
VERSION     DAORVN010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2321
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46754_length_2321_cov_4.122683"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1636)
                     /locus_tag="PKV23_07260"
     CDS             complement(<1..1636)
                     /locus_tag="PKV23_07260"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HQX85268.1"
                     /translation="MSGTLYTDGAQHPIRIAQRRIAEVFARGASLPRSESATGLLSCV
                     VPIAEAVGWRGAPRQIAEAMPHEAPVTDVAMLRTVLFRLGIETEKINVPAHRIRDEYC
                     PCLAVSKTDSLVFVQSVDTHGTARVFDPQTAAWKNVKRLKLVGEVYIVRLLDPFAQQE
                     RLQRDGFVWPLLRRFSDSLKMVFWQSLAINLLGLVVSVYVMFVYDKAIGGKSLDTLAL
                     FFIGGLAAVGLELRLRHRRAAAIARLGARFDALASTGAFQTVLGLPLGMSENAPLSAQ
                     LTRFRQLEVGRELFGGSLATSLIDLPFTLIFFILIFVLGGALGFVPVAFGVVLMLVGL
                     GTTPGLSKQMRDMGDWKAKSDSLLIEICTRLKLIRADNAEDVWLHRASESYRNYLICK
                     FQSQQQGNTLQILGQAGVTISATAVLWLGTLEVMQGKLSIGALIAVMAVVWRVLGPIQ
                     TVFLSMHRIRMITGTIRQLDRLIKIKPEREQGRITGSALAVSGKMSLAGVFFRYANRP
                     DLTIKGISLDIAAGEFITITGPSGAGKSTLLKIMLGL"
     gene            complement(1633..>2321)
                     /locus_tag="PKV23_07265"
     CDS             complement(1633..>2321)
                     /locus_tag="PKV23_07265"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQX85269.1"
                     /translation="SFQYAHGSRMILDRVSMAVPAGGILAITGRDGVGKSTLVDLICG
                     YLPDYSGEIRVGGFDPRIDGKMLKQYLAVVRPGASMMRGTIMDNLIMFRKGDEIELAI
                     NAAKLIGLDAQISKLPLGYQTVMSDGPSAELPSGLVQRIAIARAIARRPAVLMLDEAN
                     GALDMAGDQLLASGLRRLKGFTTILIVTNRPSFAAIADETWRLEAGKLELVPPVLPAV
                     VPSPAHKAVP"
BASE COUNT          510 a          689 c          712 g          410 t
ORIGIN      
        1 aaaggcccag catgatcttc agcagggtcg acttgcccgc gccgcttggc ccggtgatgg
       61 tgatgaattc gcccgccgcg atgtcgagcg aaatgccctt gatggtcagg tcgggccggt
      121 tggcgtagcg gaagaaaacc cctgccaggg acatcttgcc ggaaaccgca agggcagaac
      181 cggtaatgcg gccctgctcg cgctcaggct ttatcttgat cagccggtcc aattgccgaa
      241 tggttccggt tatcatcctg atccggtgca tggagaggaa tacggtctgg atgggtccca
      301 ggaccctcca cacaacggcc atgacggcaa tgagggcgcc aatggaaagc tttccctgca
      361 tgacttcgag ggtgcccagc caaagcacgg cggttgcgga aattgtgacc ccggcctggc
      421 cgaggatttg cagcgtattg ccttgctgct ggctctggaa cttgcagatc aagtaattgc
      481 ggtaggattc ggaggcgcgg tggagccaga catcttcagc attgtcggcc ctgatgagct
      541 tgagccgcgt gcagatttca atgaggaggg aatcggattt cgccttccag tcgcccatgt
      601 cgcgcatttg cttggacagg cctggcgtgg tgcccaggcc gaccagcata aggaccacgc
      661 cgaaggccac cgggacaaag ccaagggcac cgcccagcac aaagatcaaa atgaaaaata
      721 tcagggtgaa gggcaaatcg atcagcgatg tcgcgaggct gccgccgaac agctcgcgcc
      781 ccacctccag ttgccggaag cgggtaagct gggcggaaag cggcgcgttc tcactcatgc
      841 caaggggcag gccgaggacg gtctgaaatg ctccggtgga ggccagcgca tcaaaacgcg
      901 ctcccagccg tgcaattgcc gcggcacggc ggtgtcgcaa gcgcagttcc aagccgaccg
      961 ccgccaggcc tccaatgaaa aagagggcca gcgtgtcgag cgattttccg cctatcgcct
     1021 tgtcataaac gaacatgaca taaaccgaaa cgacgaggcc aagcagattg attgccaggc
     1081 tttgccagaa caccatcttc aagctatcgc tgaagcggcg gagaagcggc cagacgaagc
     1141 cgtcgcgctg caagcgttcc tgctgcgcga atgggtcaag caaacgcaca atatagactt
     1201 caccaaccag tttcagccgt ttcacattct tccaggcagc agtttgcgga tcaaagaccc
     1261 gggcggtgcc gtgcgtgtcc acggactgga cgaaaaccag gctgtcggtc ttgctgacag
     1321 cgaggcaggg acaatactca tcacgtatgc ggtgggccgg aacattgatt ttttccgttt
     1381 caatgccaag gcgaaacaaa accgtgcgga gcatggccac atcggtgacg ggtgcctcat
     1441 gcggcatggc ttcggcgatc tgccgcggcg ctccccgcca gcccacggcc tcggcaatgg
     1501 gaacgacgca ggacaaaagg ccggttgcac tttcggaacg cggaagcgag gcgccacggg
     1561 cgaaaacctc ggcgatcctg cgttgcgcaa tccggatggg atgctgcgcg ccatcagtgt
     1621 acagcgtccc gctcatggca cggccttgtg ggcaggggac ggcacaaccg cgggcaggac
     1681 tggcggcacc agttcaagct ttcctgcctc aaggcgccat gtctcatcgg caatggcggc
     1741 aaaggacggg cggttggtga cgatcaggat cgtcgtaaat cccttcagcc ggcgcaatcc
     1801 gctggccagc aactggtcgc cagccatatc aagcgcaccg tttgcctcat ccagcatcag
     1861 gaccgcagga cgcctggcaa tggcgcgcgc tatggcgatg cgctgcacca ggcccgaggg
     1921 cagctccgcc gaggggccat cggacatcac cgtctggtag ccgagcggca acttggaaat
     1981 ttgcgcatcg agcccaatca gcttggctgc atttatggcg agctcgatct cgtcaccctt
     2041 ccggaacatg atgagattgt ccatgatggt accacgcatc atcgaggccc cgggacggac
     2101 gaccgcgaga tactgcttga gcattttgcc gtcaattctg ggatcgaagc ccccgacgcg
     2161 gatttccccg ctgtaatccg gcaaatagcc acagatcagg tccaccagag tcgattttcc
     2221 cacaccatcg cggccggtga tggcaagaat gccgccagcg ggcacggcca ttgacaccct
     2281 gtcgaggatc atgcggctgc cgtgagcata ttggaaggaa a
//
LOCUS       DAORVN010000336         2316 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_46937_length_2316_cov_2.490491, whole genome shotgun sequence.
ACCESSION   DAORVN010000336 DAORVN010000000
VERSION     DAORVN010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2316
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46937_length_2316_cov_2.490491"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..878
                     /locus_tag="PKV23_07270"
     CDS             <1..878
                     /locus_tag="PKV23_07270"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HQX85270.1"
                     /translation="HDANIAPWLLLSDDLGLVVRWMDFDPETYEFPEDALRKVLSDKT
                     RLVALGLASNCTGTVNDVKAFAREAKAAGALVYVDAVQFAPHYTIDVQELGADVLVSS
                     AYKWFGPHMGVLWGREELLKETFSYKVRPAGDDLPHKFETGTLSHEGMAGCLGAIEYL
                     EQFGAGETRAKRISSAWDKLVVYEQKLTLQLIDGLRGVKGLTIRGLTSANAMHRRVPT
                     VSFTLDGHHPDNLAKGFAAENMFVWSGHNYAIEPVDRMGLLDKGGVLRVGLAHYNTAA
                     EVDSFLSALTRIIKN"
     gene            889..1818
                     /locus_tag="PKV23_07275"
     CDS             889..1818
                     /locus_tag="PKV23_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014129415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HQX85271.1"
                     /translation="MVFRIAVVGLGKIATDQHLPCIAKNRNFKLVAGVSRHAKVEQVA
                     CFESLSQLRASKIAVDCVALCTPPAVRLAMAREALDYGWHLLIEKPPTPTVGELLAME
                     AYARKKKRVLYAAWHSRYNRSVDMAKAKLKGQHVNFLKVTWKEDVRKWHPGQEWIWQP
                     GGFGVFDPGINALSIVTKILPEPIFADSATIEVPANASTPIAADIRFKRGDGAAADLS
                     AAFDWRQETGETWEIEIGTKSGLALHLKKGGSVLIVNGKIAHEAVLHEYEDIYAKFAR
                     LLKAKKSEVDASPLQLVSDCFMLGRPVTTAEFS"
     gene            complement(1796..>2316)
                     /locus_tag="PKV23_07280"
     CDS             complement(1796..>2316)
                     /locus_tag="PKV23_07280"
                     /inference="COORDINATES: protein motif:HMM:NF025052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HQX85272.1"
                     /translation="VCVGGLSGSGKSTLAARLAHDIGLAPGALHVRSDVERKVMAGVD
                     ETMHLPPESYSAAASAANYEAIFQRAGAALKAGHSVVLDAVFANEGERDAAQELAKSL
                     NVRFTGLWLAAPPDVLKSRVMERKGDASDADVAVVEKQLGYQPGEIRWEKVDASGSLN
                     ASEALVRKILRL"
BASE COUNT          476 a          673 c          702 g          465 t
ORIGIN      
        1 accacgacgc caacatcgcc ccctggctgc tgctgtcgga cgacctgggg ctggttgtcc
       61 gctggatgga tttcgatccg gagacctatg agttcccgga agatgcgcta cgcaaggtgc
      121 tgtcggacaa gacgcggctc gtggctttgg ggctggcctc caattgcacc ggaacggtca
      181 atgacgtgaa ggcttttgcc agggaggcca aggccgcggg cgcgctggtt tacgtggatg
      241 cggtgcaatt cgcgccgcat tacaccatcg acgtgcagga gctcggggct gatgtcctgg
      301 tttcttccgc ttacaaatgg tttggccccc atatgggcgt gctgtggggg cgcgaggaac
      361 tgctgaagga aaccttcagt tacaaggtcc ggcctgcggg tgatgatctg ccgcacaagt
      421 ttgaaacggg caccctgagc catgagggca tggcgggctg ccttggagcc atcgaatatc
      481 tcgaacaatt cggcgctggc gagacgcgcg cgaaaaggat ttcaagcgcc tgggacaagc
      541 ttgtggttta cgaacagaag ctcacgctgc agctgattga cggtttgcgc ggggtgaagg
      601 gccttaccat tcgcgggctt acctcggcca acgccatgca tcgccgggta cccactgtat
      661 ctttcacgct cgacggccac catcccgata atcttgccaa gggcttcgcg gcggagaaca
      721 tgttcgtctg gtccggtcac aactatgcca tcgagccggt ggaccgtatg gggctgctgg
      781 acaagggcgg cgtgctgcgc gtggggctgg cgcattacaa cacggcggcg gaagttgaca
      841 gttttctctc cgctttgacc cgcatcatca agaactagga atttgcacat ggttttcaga
      901 atcgccgtcg tcggactcgg caagatcgca acggaccagc acctgccctg tattgccaag
      961 aaccggaact tcaaattggt ggctggcgtc agccgtcacg caaaagtcga acaggttgcc
     1021 tgttttgagt ccctgagcca actgcgggca agcaagatcg cggtggattg cgtggcgctc
     1081 tgcacgccgc ccgcggtgcg gctcgccatg gcacgggaag cgctggatta tggctggcat
     1141 cttctcatcg aaaagccgcc aacccccacg gtgggcgaat tgcttgcgat ggaggcttat
     1201 gcgcgaaaaa agaagcgcgt gctctacgcc gcctggcatt cgcgctacaa caggtccgtg
     1261 gatatggcaa aagccaaact caagggccag catgtgaatt ttctgaaagt cacctggaag
     1321 gaggatgtgc ggaagtggca tcccggccag gagtggattt ggcagcccgg cggctttggc
     1381 gttttcgatc ccggcatcaa tgccctttcg atcgtgacca aaattctgcc cgaacccatc
     1441 tttgcggaca gcgccacgat cgaggtcccc gcgaacgcgt caacgccgat tgctgccgat
     1501 attcgtttca aacgcggcga tggcgcggcc gccgacctca gcgccgcatt cgattggcgt
     1561 caggagaccg gcgaaacctg ggaaatcgaa attggcacga aaagcggatt ggcccttcat
     1621 ctcaaaaagg gagggtcagt cttgatcgtc aacggcaaga tcgcccatga ggccgttctg
     1681 catgagtatg aagacatcta cgccaagttc gcgcggcttc tgaaagcgaa gaaatccgaa
     1741 gtggatgcct cgccgcttca actcgtaagt gattgcttca tgctgggcag gccggtcaca
     1801 accgcagaat tttcctgacg agggcttcgg aagcatttag gcttcccgag gcatccacct
     1861 tctcccagcg gatttctccg ggctgatagc cgagctgctt ttcgacgacg gcgacgtccg
     1921 catctgatgc atcgcctttg cgttccatga cgcgcgactt gagaacatcc ggcggcgctg
     1981 caagccacag gcctgtaaac cgcacattca gactcttggc caattcttgc gcggcgtcgc
     2041 gctcaccctc gttggcaaac accgcgtcaa ggacgacgga atggccagcc ttgagagctg
     2101 ccccggcacg ctggaatatt gcctcatagt ttgcggcgga cgcggcagcc gaataggact
     2161 caggtggcaa atgcatagtc tcatcgacgc ctgccatgac cttgcgttcg acatcgctgc
     2221 gcacatgaag tgcgcccggc gccaagccga tgtcatgggc cagccgtgcc gcaagagttg
     2281 acttgccgct tcccgacaac ccgccgacgc agacaa
//
LOCUS       DAORVN010000337         2311 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_47136_length_2311_cov_2.891844, whole genome shotgun sequence.
ACCESSION   DAORVN010000337 DAORVN010000000
VERSION     DAORVN010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2311
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47136_length_2311_cov_2.891844"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..573
                     /locus_tag="PKV23_07285"
     CDS             <1..573
                     /locus_tag="PKV23_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011582461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HQX85273.1"
                     /translation="CDVFRGLPAVVTLYLIGFGIPISGLPDLIVPKVMGLFIDLSAMT
                     PAELKTATRIPVIWWCILALTLTYGAYVAEVYRSGIESIHWSQVSAARSLGLSYMQTM
                     RYVVIPQAVRRIMAPLLNDFIGLQKDTALVQVVGVIDAFNQSRIIASNAFNLSAVTIV
                     AILFVLITIPQARFVDRLIERDNARTRAGG"
     gene            575..1318
                     /locus_tag="PKV23_07290"
     CDS             575..1318
                     /locus_tag="PKV23_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007880315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HQX85274.1"
                     /translation="MAFLEMKNVEKEFGPVRVLRGINLEVNEHQVVCLIGPSGCGKST
                     LLRCINGLEKIQGGEIRLEDDRVSGPGVDVNKLRQQVGIVFQAFNLFPHMSVLENVTL
                     APTKVLKMAKDAADDRAMVLLKRIGLESKAQEFPDRLSGGQQQRVAIVRALAMEPKLM
                     LLDEITSALDPELVSEVLNIVRDLAQQGMTMLLATHEMGFAREVASKVCFLYEGVVHE
                     EGPPDQLFGKPREERTRAFLKRIIEAGRL"
     gene            1478..>2311
                     /locus_tag="PKV23_07295"
     CDS             1478..>2311
                     /locus_tag="PKV23_07295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85275.1"
                     /translation="MKLLPLTASLMALALYSAPAFAQEEVPVQEQPAVEEAAPQVPEE
                     VLVLLNDPRPLSELSVDELGARAKQARTFSKMKALPAETRDQLQAMAQAARGEIAARE
                     QQAAQPEAPAAEQPAAPEVAQQPAVEAPAAQPAEDAGPVAVQLPPDVATLMNDQRPLS
                     ELSAEELDHRFKTARQFSRAKQLPEETKAQLAGMAKAAREELMARQSATQPVPEQPET
                     AAQPPAAEQPAVVEQAPVVGQPPVAEAPPPAAAPPADAKQSQQLDGNAAVPEAEAKAQ
                     AY"
BASE COUNT          447 a          744 c          690 g          430 t
ORIGIN      
        1 tgcgatgtct tccgcggcct tcccgctgtt gtcacgctgt atctcatcgg cttcggcatt
       61 cccatttccg gcctcccgga cctcatcgtg ccaaaggtca tgggattgtt catcgacctc
      121 tccgccatga ctccggccga actcaagacc gccacgcgca tcccggtgat ctggtggtgc
      181 atcctcgccc tcacgctcac ctatggcgcc tacgtggccg aagtctaccg ctccggaatt
      241 gaaagcattc actggagcca ggtttccgcc gcgcgctccc ttggcctctc ctacatgcag
      301 accatgcgct atgtcgtcat cccgcaagcc gtgcgccgca tcatggcgcc gctgctcaat
      361 gatttcatcg gactgcagaa ggacaccgcc ttggtgcagg tggtgggcgt catcgatgcc
      421 ttcaaccagt cgcgcatcat tgcctccaac gccttcaatc tttccgccgt gaccatcgtg
      481 gcaatcctct ttgtgctgat taccattccg caggcccgct tcgtcgacag gctcattgag
      541 cgcgacaatg cccgcacccg ggcagggggc tgacatggcg tttctggaaa tgaagaacgt
      601 tgaaaaggaa tttggcccgg tgcgcgtgct gcgcggcatc aatctggaag tgaacgagca
      661 tcaggtcgtc tgcctcatcg gcccatcggg ctgcggaaag tccacgctgc tgcgctgcat
      721 caacgggctg gaaaaaatcc agggcggcga aatccgcctt gaggacgacc gcgtgtcggg
      781 gccgggagtg gacgtgaaca agctgcgcca gcaggtcggc attgtgtttc aggccttcaa
      841 cctgtttccc cacatgagcg tgcttgagaa cgtgacgctg gccccaacca aggtcctgaa
      901 gatggcgaag gacgccgccg atgatcgcgc catggttctc ctgaagcgca ttggcctgga
      961 gagcaaggcc caggaattcc ctgaccggct ttccggcggc cagcagcagc gcgtggccat
     1021 tgtccgggcc ctggccatgg aacccaagct catgctgctc gatgagataa cctccgccct
     1081 tgacccggaa ctcgtgtcgg aagtcctgaa cattgtgcgc gacctggccc agcagggcat
     1141 gaccatgctg ctggcgaccc atgaaatggg ctttgcccgc gaggtggcct ccaaggtgtg
     1201 ttttctctat gaaggcgtgg tgcacgagga gggcccgccg gaccagcttt tcggcaagcc
     1261 ccgggaagag cgcacccggg cctttctgaa gcgcattatc gaggccggcc ggctgtaatt
     1321 caggtcttgg gtggaaccat tgtcattaaa ggacgtttat atttgtggca gaagtgcgaa
     1381 ccaatcccat agttctgccg cataggtccc ctagttgcgg ggcagaatca acattttggc
     1441 gtacattttg gttacgtcgc atcctcggag gatctacatg aagttgctac ccctgactgc
     1501 cagcctgatg gctttggctc tgtattcggc gcccgcgttc gcgcaggagg aagttccggt
     1561 ccaggaacag cctgccgttg aagaagcggc gccgcaagtt ccggaagaag ttctggtcct
     1621 gctcaatgac ccccggccgc tttccgagct ttcggtagat gaactcggtg cgcgcgccaa
     1681 gcaggcccgc acgttctcaa agatgaaagc tcttccggcc gaaacgcgtg accagttgca
     1741 ggccatggcc caggcagcgc ggggcgaaat cgccgcccgc gaacagcagg cggcccagcc
     1801 tgaagcgccg gcagccgaac agccagcagc acctgaggtt gcccagcagc ccgcggttga
     1861 agccccggca gcccagcctg ccgaagacgc aggcccggtt gccgtgcagc ttccgcccga
     1921 tgtggccacg ctcatgaacg atcaacggcc gttgagtgaa ctgagcgccg aggaactcga
     1981 tcaccggttc aagacggctc gccagttctc cagggccaag cagcttccgg aagaaaccaa
     2041 ggcgcaattg gccggaatgg caaaagcggc ccgcgaagaa ttgatggcgc gccagtcggc
     2101 cacccagccg gtgccggagc agcccgaaac cgctgcccag ccaccagcag ccgaacagcc
     2161 cgctgttgtg gagcaggctc cggttgtcgg gcagccgccc gtagccgaag caccgccgcc
     2221 agccgccgcg ccaccggctg atgcaaagca atcccagcag cttgacggca acgccgcggt
     2281 tcccgaggcg gaagccaagg ctcaagccta t
//
LOCUS       DAORVN010000338         2310 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_47154_length_2310_cov_4.515743, whole genome shotgun sequence.
ACCESSION   DAORVN010000338 DAORVN010000000
VERSION     DAORVN010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2310
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47154_length_2310_cov_4.515743"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(82..1329)
                     /locus_tag="PKV23_07300"
     CDS             complement(82..1329)
                     /locus_tag="PKV23_07300"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HQX85276.1"
                     /translation="MSAPQLVESFFRHEYSRVVAMLARRVGMANIALVEDAVQSTMML
                     ALERWKVAGVPDQPTAWMYRVAYNAVIDALRKQARQERILMKEADLEHVASERNPESL
                     QSWEVNDAMLRMLFVCCDDVLPAQSQLIMALKTLCGFDTREIAQRLFISEENVYKRLG
                     RARIAFRAEAQHFDQLGVEQYAARVPAVRNVIYTLFTEGYLSSHAELAIRQELCTEAI
                     RLATMLANHGVGQDPETFALLALLHLHAARLASRQTAAGDLLLLQEQDRGLWDWQMIE
                     TGLTWLAKSAEGDVFSRYHAEAGVAAEHCLAPSIEATNWERIAGYYEQIERLSKSPIH
                     RLNRAVAVAQWKGPAAGLAVLQDFEIPTWLAGSYMWHAVLADLHRRCGDTRQAQTYME
                     LACAAAPSEAVRSLLLRRLQVDG"
     gene            complement(1326..1661)
                     /locus_tag="PKV23_07305"
     CDS             complement(1326..1661)
                     /locus_tag="PKV23_07305"
                     /inference="COORDINATES: protein motif:HMM:NF015735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HQX85277.1"
                     /translation="MKSYLCLQRNLPGGEQRQPSPTQMQEMYAKFGEWQAKFKSNITD
                     MGGKLGAGKVVGKDQALDGPFVEVKDLVGGYMIIAANSLDEAAVVANECPGLVGPGSG
                     CEIIEIHRP"
     gene            2045..>2310
                     /locus_tag="PKV23_07310"
     CDS             2045..>2310
                     /locus_tag="PKV23_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015917738.1"
                     /note="has catalase and peroxidase activities; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase-peroxidase"
                     /protein_id="HQX85278.1"
                     /translation="MTTDNGKCPIPGHAGSTAGGRGTGNRNWWPNQLNLGILRQHAPA
                     ANPMGPGFNYAEAFKKLDLAALKNDLRALMTDSQDWWPADWGHYG"
BASE COUNT          459 a          760 c          633 g          458 t
ORIGIN      
        1 acgatctaca tgcgcgaaat ccctagttcg ctctatcgac gggcgcaatc gattgcgatt
       61 gccgaggccc gtttccgccg ctcaaccgtc cacttgcagc ctacgcaaca aaagagatcg
      121 aaccgcctca ctgggcgccg cggcgcacgc cagctccata tatgtctgcg cctggcgcgt
      181 gtcaccgcag cgccggtgca aatcggccag gaccgcatgc cacatgtagg accccgccag
      241 ccatgtcgga atctcgaaat cctgcagcac ggcaagcccc gccgccggtc ccttccactg
      301 cgccaccgcc actgcgcggt tcagccggtg aatcggtgat ttcgataggc gctcaatctg
      361 ttcgtaataa ccggcgatgc gttcccagtt ggtcgcctca atcgacggcg caagacagtg
      421 ttcagccgcg acaccggcct cggcatgata gcgagaaaac acatcgccct cggcggactt
      481 tgccagccat gtcaagcctg tttcgatcat ctgccaatcc cacaggccgc ggtcctgctc
      541 ctgcagcaga agcaagtcac ccgccgcggt ctggcgtgag gccaggcgcg cagcatgcaa
      601 gtgcaatagc gccagcaagg caaacgtttc aggatcctgc ccaaccccat gattggccag
      661 catggtggcc aggcggatgg cctcagtaca gagctcctgt cgaatggcca gctcagcatg
      721 cgacgacagg tagccctcgg tgaacaacgt atagatgaca ttcctcacgg cgggtacccg
      781 ggccgcatat tgttccacgc cgagctgatc aaaatgctgc gcctccgccc ggaaagcgat
      841 acgtgcccgg cccaggcgct tgtagacatt ttcctcgctg atgaacaatc gttgcgcgat
      901 ttcacgcgtg tcaaagccac acagcgtctt caaggccatg atcagctgcg actgggcggg
      961 cagcacgtca tcacaacata cgaacaacat gcgcagcatc gcgtcgttga cttcccacga
     1021 ttgcagcgat tccgggttgc gctccgaagc cacgtgctca aggtcggctt ccttcatcaa
     1081 gatacgctct tgtcgcgcct gtttgcgcag cgcgtcgatg accgcgttgt aggccacgcg
     1141 atacatccag gcggtcggct ggtcaggcac acccgccacc ttccaccgtt ccagcgcgag
     1201 catcattgtc gactgcaccg cgtcttcgac cagcgcaatg ttcgccatgc ccacccgccg
     1261 ggcaagcatg gcgaccacgc gcgaatactc gtgcctgaaa aagctttcga cgagctgggg
     1321 agcactcaag gacgatggat ctcgatgatt tcgcagccgg agcctggccc gaccagaccg
     1381 gggcattcat tcgcgaccac cgctgcttcg tccaggctgt tggcggcgat gatcatgtag
     1441 ccgcccacaa ggtccttcac ctcaacaaag gggccatcaa gcgcttggtc cttcccgacc
     1501 actttcccag cgcccagttt gccgcccatg tccgttatgt tggacttgaa tttcgcctgc
     1561 cactccccga atttggcata catttcctgc atttgggtgg gtgaaggttg gcgctgttcg
     1621 ccgcctggca gattacgttg cagacacaga tagcttttca tgatgtttac tccttgattc
     1681 ttacccagtc attcttggct gttatctata tgacgtgcga tccttccaca attggacaca
     1741 attgtcaggg ttttgcccat tgtccggtcg cgcgccgact ttcgcctgga gcgcgaagtc
     1801 tgggtcccgt cctccgccac ccgaaccata ccagtctgac gcactggagc gggccattac
     1861 ctttcgctgg ggtgattgaa atgagcgcgg ctcagttttg cgcgcctggc agcttcttcc
     1921 gtgtcacaat atcgatattg gcgaaatgaa ccccctcttt cgggagtgat gtccccgggc
     1981 tccgcccggg acaccgtttc cgaagaacgg ttctgtaagg ggttctccac cccaaaggag
     2041 cacgatgaca accgacaatg gcaaatgccc gatccccggg cacgccggca gcaccgccgg
     2101 cggcagaggc actggaaacc gcaactggtg gcccaatcaa ctcaacctgg gcatcctgcg
     2161 ccagcacgcg ccggctgcca acccgatggg tcccggcttc aactacgccg aggccttcaa
     2221 gaagctggat ctggccgcct tgaagaacga cctgcgggcg ctgatgaccg actcgcagga
     2281 ctggtggccg gccgactggg gccattacgg
//
LOCUS       DAORVN010000339         2309 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_47200_length_2309_cov_2.867347, whole genome shotgun sequence.
ACCESSION   DAORVN010000339 DAORVN010000000
VERSION     DAORVN010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2309
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47200_length_2309_cov_2.867347"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..523)
                     /locus_tag="PKV23_07315"
     CDS             complement(<1..523)
                     /locus_tag="PKV23_07315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HQX85279.1"
                     /translation="MNHLLCFGFGFSARALAARLDRREWKVSATSRDAEGIAVIEAQG
                     FHGLLFDSTLQIGPDVTHLLISAPPGEHGDPVLRLFQAQLQQLSKSLKWVGYLSTTGV
                     YGDRGGDWVDEDSPLEPTTARGQRRLEAERNWLKLHKDFGLPVHLFRLAGIYGPGRNQ
                     LLTVRDGSARRIIK"
     gene            complement(520..1635)
                     /gene="queG"
                     /locus_tag="PKV23_07320"
     CDS             complement(520..1635)
                     /gene="queG"
                     /locus_tag="PKV23_07320"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009450946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HQX85280.1"
                     /translation="MPTSISERLRARASQEGFSGIRIASADAGPEQGERLRSFLEKSY
                     HGEMDWLAETAERRAAPKSLWPKAQSAIVLAMNYGQEIDAFERLERTSQGVISAYALN
                     RDYHDVVKGKLKKIATWFASATKSEVKVFVDTAPLMEKPLAHRAGLGWQGKHTNLVSR
                     ELGSWFFIGTILTDKALPEDGTETDHCGSCQACLDICPTKAFPAPYQLDARRCISYLT
                     IEHKGHIDREFRKPMGNRIYGCDDCLAVCPWNKFASEASEAKLRAREDLVSPGLDELA
                     QLDDAAFRKLFVGSPVKRVGRDRFVRNVLIAIGNSGNPDFVPVAEHLLRDASPLVRAM
                     AVWALAQLMPQEDFQQARNMHVMTEPDLAVKAEWSSP"
     gene            complement(1616..2299)
                     /locus_tag="PKV23_07325"
     CDS             complement(1616..2299)
                     /locus_tag="PKV23_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013912711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HQX85281.1"
                     /translation="MPKLLHFPLDPFSRRVRLALAEYGVAFELAEERPWTPSERLFNL
                     NPAGIVPVFVEDDGSAISGIEAITEYLEETRAGKTKLIPGQAGGRAEVRRLTGWFDTK
                     FYAEVSEPIITEKIIRRFMTRETGGGAPDMARVRYGLSRLRDHLDYMGTLADHRSWLA
                     GDELSIADLSAAAHLSVIDYLGDIPWTDFPVAKLWYQRIKSRPSFRPLLGDTLRGVPA
                     SASYADLDF"
BASE COUNT          469 a          758 c          643 g          439 t
ORIGIN      
        1 gcttgatgat gcgcctggcg ctgccatccc gcacagtgag aagctggttc cgccccggcc
       61 cataaatccc cgccaggcga aacagatgta ccggcagtcc aaaatccttg tgaagcttga
      121 gccagttccg ctccgcctcc aggcgcctct ggccccgcgc cgtagtgggt tcgagcggac
      181 tgtcctcgtc aacccagtca ccaccccggt cgccgtacac ccccgtggtt gagagatagc
      241 ccacccactt caaacttttc gaaagctgct gcaattgcgc ctgaaacaag cgcaggacgg
      301 gatcgccgtg ttcacccgga ggtgccgaaa tcagaagatg cgtaacatca ggtccaatct
      361 gcagagtgct gtcaaacaat agtccatgaa atccctgcgc ctcgatcacg gcaatgcctt
      421 ctgcatcacg gctcgttgcg gaaaccttcc attcccgcct gtccagccgc gccgccaaag
      481 cccgcgccga aaagccgaag ccgaaacaca ggagatggtt cacggtgaac tccattcggc
      541 tttcactgcc agatcaggtt cagtcatgac atgcatattg cgtgcctgct gaaaatcttc
      601 ctgaggcatc aattgtgcca gcgcccacac ggccatggcg cgtaccagcg gagaagcatc
      661 acgcaaaaga tgttccgcca ccggaacgaa atccggattg ccgctgtttc cgatcgcgat
      721 cagcacattg cgtacgaaac ggtcgcgccc aacccgtttc accggggaac ccacaaacag
      781 cttccggaat gccgcatcat ccaattgagc cagttcatca agtcccggcg aaaccagatc
      841 ctcacgggcc cgcagcttcg cctcgctcgc ctcgctggca aacttgttcc acgggcacac
      901 cgccaggcag tcatcgcagc cataaatgcg gtttcccatg ggcttgcgga attcgcggtc
      961 gatatggccc ttgtgctcga tggtgagata ggaaatgcag cgccgcgcat ccagctggta
     1021 gggtgcggga aaggccttgg ttgggcagat atcaaggcag gcctggcagg agccgcaatg
     1081 gtccgtctca gtgccatctt cgggcagggc cttgtcggtc aggatcgtgc cgatgaaaaa
     1141 ccatgatccg agttcccgcg acacgagatt tgtatgcttg ccctgccagc cgagccccgc
     1201 cctatgggcc agcggctttt ccatcagcgg cgccgtatcc acgaaaactt tcacctccga
     1261 ctttgtagcg ctcgcaaacc atgtggcaat tttcttgagc ttgcccttga ccacgtcgtg
     1321 atagtcgcgg ttcagcgcat aggcggaaat caccccctgc gacgtccgct ccagccgttc
     1381 gaatgcatca atctcctgcc cataattcat ggccagcacg atggctgact gcgccttcgg
     1441 ccacagggat ttcggcgccg cgcggcgctc cgccgtttcg gcaagccagt ccatctcgcc
     1501 gtggtaactc ttttcaagga agctgcgcag tctttcccct tgttcggggc cggcatcggc
     1561 tgaggcaatc ctgatcccgc tgaagccttc ctgcgaggcc cgggcccgca gccgttcaga
     1621 aatcgaggtc ggcataggaa gcagatgccg gcacgccgcg cagcgtgtcg ccgagcagcg
     1681 gacggaatga cgggcgcgac ttgatccgct ggtaccacag cttcgccacc ggaaagtccg
     1741 tccacggaat atcaccgaga tagtcgatga ccgagagatg cgccgccgcc gaaaggtcgg
     1801 caatggaaag ttcatccccc gccagccacg agcggtggtc cgccagggtt cccatgtagt
     1861 cgagatggtc cctgagccgc gaaagcccgt agcgcacccg cgccatatcg ggcgccccgc
     1921 cgccggtttc acgcgtcatg aagcgccgga tgatcttttc cgtaatgatt ggctctgaaa
     1981 cctccgcata gaacttcgtg tcaaaccagc cggtgagccg ccgcacctcg gcgcggccgc
     2041 ccgcctgtcc cggaataagc ttggtttttc ccgcgcgcgt ctcttccaga tattcggtga
     2101 tcgcctcgat gccgctgatc gccgagccat cgtcttcaac aaaaaccggc acgatgcccg
     2161 ccggattgag gttgaacagc cgctcgcttg gcgtccatgg ccgctcttcg gccagctcga
     2221 aggccacgcc atattccgcc agcgccaggc gcacccggcg gctgaacgga tcgagcggaa
     2281 agtgaagcaa tttgggcatg ggatgaagt
//
LOCUS       DAORVN010000340         2304 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_47369_length_2304_cov_3.550467, whole genome shotgun sequence.
ACCESSION   DAORVN010000340 DAORVN010000000
VERSION     DAORVN010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2304)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2304)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2304
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47369_length_2304_cov_3.550467"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1656)
                     /gene="topA"
                     /locus_tag="PKV23_07330"
     CDS             complement(<1..1656)
                     /gene="topA"
                     /locus_tag="PKV23_07330"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018390664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HQX85282.1"
                     /translation="MTVVVVESPAKAKTINKYLGPDFTVLASYGHVRDLPAKDGSVRP
                     DEDFAMSWEVDSKSAKRMADIADALKDDDRLILATDPDREGEAISWHVLEILKKKGAL
                     KGKTIHRVVFNAITKQSVLEAMHHPRDVDGPLVDAYLARRALDYLVGFTLSPVLWRKL
                     PGSRSAGRVQSVALRIICDRELEIEAFKRQEYWTIDGTFTTPQGQEFSATLQAIAGQK
                     IGKLDIKDEASARAIEQTLKNARVSVDNVESKPAKRHPYAPFRTSTLQQEASRKLGFS
                     SAKTMQVAQRLYEGVDIDGDTVGLITYMRTDGVDIAPEGIAAARNAILKNFGEPYLPS
                     SPRQYTTKAKNAQEAHEAIRPTELGRLPASVRSALDGEQFALYELIWKRTIASQMESA
                     EFERTSVDLGATGLDGKHYTFRANGSVVKFDGFLKVYQEDLDDGEDEDSRRLPAMARG
                     DGIGVRKVSAEQHFTEPPPRYSEASIIKKLEELGIGRPSTYVSVLSTIRDRGYVRLDK
                     KRFIPEDKGRVVTAFLESFFSKYVEYGFTANLEEQLDRISNGEI"
     gene            1852..2091
                     /locus_tag="PKV23_07335"
     CDS             1852..2091
                     /locus_tag="PKV23_07335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85283.1"
                     /translation="MNDPYSKTPAKDAKNQELAQYIADMLLELRKMAKSAGLNTLLGL
                     LELSFCEAFSIANKVEIPPGEIEKLRRLAREAGEA"
     gene            complement(2057..>2304)
                     /locus_tag="PKV23_07340"
     CDS             complement(2057..>2304)
                     /locus_tag="PKV23_07340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85284.1"
                     /translation="LIVRREFGQGTLRVIEGNAGDTLGSENSLHLVYVLEGECRANDL
                     KANDSVYLGRADFLALPTPMKLAVCEVTPRRLPVPGA"
BASE COUNT          476 a          674 c          663 g          491 t
ORIGIN      
        1 gatttcaccg ttggagatcc ggtcgagctg ctcttcgaga tttgctgtga agccatattc
       61 cacatatttg ctgaagaagg attcgaggaa cgctgtgacc acgcggccct tgtcttccgg
      121 aatgaaacgc ttcttgtcca ggcgcacata gccccggtcg cggatcgtcg aaagtaccga
      181 aacataggtc gagggccggc cgatgccgag ctcctccagt ttcttgatga tggaagcttc
      241 cgaatagcgc ggtggcggct cggtgaaatg ctgttcggcg gaaaccttgc ggacaccgat
      301 gccatcgccc cgcgccatgg cgggcagacg gcgcgaatcc tcgtcttccc catcgtcaag
      361 gtcttcctgg taaaccttga gaaagccgtc gaacttcact acggacccat tggcccggaa
      421 ggtataatgc ttgccatcca gccctgtggc gccaagatca accgaggtgc gctcgaattc
      481 cgcactttcc atctggctgg caatggtgcg cttccagatc aattcgtaaa gggcaaactg
      541 ctcgccatcg agggccgacc gtaccgatgc gggcaggcga ccgagctcag tcggtcggat
      601 ggcttcatgg gcctcctggg cattcttggc cttggtggtg tactggcgtg ggctcgatgg
      661 caggtagggt tcgccaaaat tcttgagaat ggcgttgcgc gccgccgcaa tgccttccgg
      721 ggctatatcg acgccatcgg tccgcatgta agtgatcagg cccaccgtat ccccatcaat
      781 gtccacgcct tcgtaaagcc gctgggccac ctgcatcgtt ttggccgaag agaagcccag
      841 cttgcgcgag gcctcctgct gcagcgtcga ggtgcggaac ggcgcatagg ggtggcgctt
      901 ggccggcttg ctttcgacat tgtccacgga aacacgggcg ttcttcaggg tctgctcgat
      961 ggcgcgcgcc gaagcctcgt ccttgatatc gagcttgccg atcttctgcc cggcaatggc
     1021 ctggagcgtc gcggaaaatt cctgcccctg gggcgtcgtg aaggtgccgt cgatggtcca
     1081 gtattcctgg cgcttgaagg cttcgatttc gagttcgcgg tcgcagatga tgcgcagtgc
     1141 caccgactgc acccggccag ccgagcgcga gccgggcagt ttccgccaca gcacggggga
     1201 cagggtaaag cccaccagat agtccagcgc ccgccgggcc agataggcat cgaccagtgg
     1261 cccgtccacg tccctcgggt ggtgcatggc ctcaagcacc gattgcttgg taatggcatt
     1321 gaataccacg cgatgaattg tcttgccctt cagggcaccc tttttcttca aaatttcaag
     1381 cacatgccat gaaatcgctt cgccttcccg gtccgggtcg gttgccagga tcagcctgtc
     1441 gtcgtccttg agggcatccg cgatgtcagc catgcgcttg gccgatttgg aatccacctc
     1501 ccaggacatg gcaaaatcct cgtctgggcg caccgagcca tccttggccg gcaggtcgcg
     1561 gacatgccca taggacgcca gaaccgtaaa gtccgggccc agatacttgt tgatggtttt
     1621 cgccttggcg ggcgattcga cgacgacaac ggtcattctt cttcctgact tggggacgga
     1681 gggcctcata taggcatctt tccgccacgg caagttacct gcgttaactg acaaatcagc
     1741 gtaagactaa cccaatacaa tcaaagattg ctttatttaa tattgaacac gcatatagtg
     1801 tgcccacaac cagagagcta gctggggcca gattgcagac tcgaggcggt catgaacgat
     1861 ccttacagca agactccggc gaaagatgcc aagaaccagg aattggccca atacattgcc
     1921 gatatgctgc ttgagcttcg gaaaatggcc aaatccgctg gcttgaatac gcttctaggc
     1981 ttgctggaac tcagcttctg tgaggcattc tccatcgcca ataaggtgga aataccgccg
     2041 ggagaaatcg aaaagctcag gcgcctggca cgggaagccg gcgaggcgtg acctcacaga
     2101 cagccagctt catcggcgtg ggaagtgcaa gaaagtcggc tcgtccgaga taaacactgt
     2161 catttgcctt cagatcattg gctcggcatt cgccttccag aacataaaca aggtgaaggc
     2221 tgttttcgga ccccagagtg tctcccgcgt tgccctcgat gacgcgcagg gttccctggc
     2281 cgaactcccg gcgcacgatc aaat
//
LOCUS       DAORVN010000341         2301 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_47501_length_2301_cov_2.370436, whole genome shotgun sequence.
ACCESSION   DAORVN010000341 DAORVN010000000
VERSION     DAORVN010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2301
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47501_length_2301_cov_2.370436"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..928)
                     /locus_tag="PKV23_07345"
     CDS             complement(<1..928)
                     /locus_tag="PKV23_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018389007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HQX85285.1"
                     /translation="MKFTRLRLSGFKSFVEPTDLLIEPGLTGVVGPNGCGKSNLLEAL
                     RWVMGESSYKSMRASGMDDVIFSGTTQRPSRNMAEVMVTMSNDDRSAPPQHNDSEVIE
                     ISRRIEREAGSAYRINGKDVRARDVQLLFADVSTGARSPALVRQGQIAEIINAKPQAR
                     RLILEEAAGITGLHTRRHEAELKLKAAEVNLTRLEDVTSQLESQLNSLKRQARQAQKY
                     KLISAEIRKLEAAGLYVAWKEAVESAAKDAAALDESTRVLAENTRAASEKLRARDELG
                     EKLPGLREQETIRAAVLQRMAVERNSLDEEERR"
     gene            complement(959..1579)
                     /locus_tag="PKV23_07350"
     CDS             complement(959..1579)
                     /locus_tag="PKV23_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HQX85286.1"
                     /translation="MIKLNKRHVLFGSAALAGLALGGASAFAQNLAGLNDPPAFGEMT
                     LGPDTAKVTVIEYASATCPHCAAFHNETFEALKKEYIDTGKIKFVFREFPHQDAALAA
                     FMLARCAPKEKYFPLIDVFFATQPEWTQNPLEGLNKIAQQAGFTKESFDACMKNETVA
                     KDILAVRSKAEGFGVTGIPTFFVNGERFEGETTIEAFRAKIDPLLT"
     gene            complement(1618..2103)
                     /locus_tag="PKV23_07355"
     CDS             complement(1618..2103)
                     /locus_tag="PKV23_07355"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HQX85287.1"
                     /translation="MERLDKHFEKLTKASFARYGFAYGELMARWPDIVGGALAQYCEP
                     ERIKWPRGAGETAQKLGGTLVIRAEPGRGLDLQYQSHQIIERINQFYGYGAVTSVKIM
                     QGHVSTAKPQKNKENSLDEDTEKALETRLEAIADDKLRKALHRLGAGALASRPGSPHD
                     K"
     gene            2156..>2301
                     /locus_tag="PKV23_07360"
     CDS             2156..>2301
                     /locus_tag="PKV23_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015915203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HQX85288.1"
                     /translation="MAEQGLHEDVSAKLLAWYDAHARELPWRSRPGIRPDPYHVWLSE
                     IMLQ"
BASE COUNT          394 a          709 c          680 g          518 t
ORIGIN      
        1 cccggcgctc ttcctcgtca aggctgttgc gctcaaccgc catgcgctgc agcaccgcgg
       61 cgcggatagt ttcctgctcg cgcaggcccg gcagcttctc gcccaattca tcgcgcgccc
      121 gcagtttttc cgaagcggcc cgcgtattct ccgccagcac gcgggttgac tcatcaagtg
      181 ccgccgcatc tttcgccgca ctctccaccg cttctttcca tgccacgtaa agccctgcgg
      241 cctcaagctt cctgatctcg gcggatatca gcttgtattt ctgcgcctgc cgtgcctggc
      301 gcttcaggct gttgagctgg ctttcaagct gtgaggtcac gtcctcgagg cgggtaagat
      361 tgacttcagc cgccttcagt ttcagctccg cctcgtggcg gcgcgtatgc aggccggtga
      421 tgcccgctgc ttcttcgagg atcaggcgcc gcgcctgcgg cttggcattg atgatctcgg
      481 caatctgccc ctggcgcacc agtgccggcg aacgcgcgcc ggttgaaaca tcggcaaaca
      541 gaagctgcac atcgcgggcc cgcacgtcct tgccgttgat ccggtaggcc gagcctgcct
      601 cgcgctcgat gcgccgcgag atttcgatta cttcgctgtc attgtgctgc ggcggggccg
      661 aacggtcgtc gttcgacatc gtcaccatga cttccgccat gttgcgcgag gggcgctgcg
      721 tcgtgccgga gaagatcacg tcatccatgc cggaagcgcg catggatttg tacgagcttt
      781 cgcccatcac ccagcgcagc gcctcgagca ggtttgattt gccgcagcca ttggggccga
      841 ccacgccggt caggcccggt tcaatcaaca ggtcggtggg ctcaacaaat gatttgaacc
      901 ccgaaagccg cagccgtgta aacttcatta tgcctcaagc cccagtcctg gccgcctctc
      961 aggtgagaag cggatcaatc ttcgcacgga aggcttcgat cgtcgtctcg ccttcaaatc
     1021 tttcgccgtt cacaaaaaat gtcggaatgc cggtcacgcc gaagccctcc gccttgctgc
     1081 gcaccgccag aatgtccttg gccaccgtct cgttcttcat gcaggcgtca aagctctcct
     1141 tggtgaagcc cgcctgctgc gcaatcttgt tgagcccttc aagcggattt tgcgtccact
     1201 ccggctgggt ggcgaagaac acgtcgatca gcgggaaata tttttccttg ggggcgcagc
     1261 gcgccagcat gaaggctgca agggctgcgt cctgatgcgg aaactcccgg aacacgaact
     1321 tgatcttgcc cgtgtcgata tactcttttt tcagcgcctc gaatgtctcg ttgtggaagg
     1381 ccgcgcaatg cgggcaggtg gccgaggcat attcgatcac cgtcaccttg gcggtgtcgg
     1441 ggccgagggt catttcgcca aaggcaggcg gatcattgag gcctgccagg ttctgggcga
     1501 aggctgaagc gccgccaagg gcgaggcccg ccaacgcggc cgaaccgaaa aggacatgtc
     1561 gtttattgag cttgatcatg gaatctacct acaagatgtt gggggttgga accaatatta
     1621 tttgtcatgt ggagaaccag gccggcttgc cagggccccg gcgcccagcc ggtgcagggc
     1681 cttgcggagc ttgtcgtcgg cgatggcttc gaggcgtgtt tcaagtgcct tctcggtgtc
     1741 ttcgtctagt gaattctcct tgtttttctg gggcttggct gtgctgacat ggccttgcat
     1801 gatttttacc gaagtcaccg ccccgtagcc gtagaactgg ttgatgcgct cgatgatctg
     1861 gtggctctgg tattgcaggt cgaggccgcg ccccggttct gcccggatga ccagggtgcc
     1921 gccaagcttt tgcgctgttt cgcccgcgcc gcgcggccat ttgattcgct ccggctcgca
     1981 gtattgggca agggctccgc cgacgatatc gggccaacgc gccatcaact cgccataggc
     2041 gaagccatag cgggcgaagc ttgccttggt gagtttctca aaatgcttgt cgagccgttc
     2101 catgtcgcgt ctatataatg cagttttaac ctgatgcgag tgttgatcga agaccatggc
     2161 ggaacaaggg cttcacgaag atgtgtcggc aaagctgctg gcctggtacg acgcccacgc
     2221 ccgcgagctt ccatggcgct caaggcccgg cataaggcct gatccctatc acgtctggct
     2281 ttccgagatc atgctgcagc a
//
LOCUS       DAORVN010000342         2297 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_47642_length_2297_cov_2.753791, whole genome shotgun sequence.
ACCESSION   DAORVN010000342 DAORVN010000000
VERSION     DAORVN010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2297)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2297)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2297
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47642_length_2297_cov_2.753791"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..660)
                     /locus_tag="PKV23_07365"
     CDS             complement(<1..660)
                     /locus_tag="PKV23_07365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85289.1"
                     /translation="MAPLSPRLGIALAFICLVILGVMPIISNGRPPGSDALTFALYLS
                     FWQLLFSIPLFARELRSNNKGIFRAGLSPARKARTLAITVTTGAIFGLSTYVYVLAFE
                     KAGAVSAAIALQAYPLFAMLLEAIFFGRMKSRGELFFTFLIIVALYYLATSGTWRIDG
                     FSFWSVFALGVPLLWSIAHVALKEVLDTTPITPNQVTFSRLLVSTAILLLAQIAFDGA
                     QG"
     gene            complement(769..>2297)
                     /locus_tag="PKV23_07370"
     CDS             complement(769..>2297)
                     /locus_tag="PKV23_07370"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018493861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HQX85290.1"
                     /translation="GVISNISDKIRWTAGYVVAWVEDEGFQQQSRKRILDAVREHRPD
                     VILAHSLGSLVTYNAFSHPDAKEEQVKRLLAKAKYVTLGSQIANPFVIRNLTNGRVLP
                     LAVSFWHHLYNIHDSVFTAPIRLWDGANFRQTETPFDVDGIADHAAESYFSHRSTIEN
                     VWRTIGAEAVGARAFGSAPTVKARAAAGSGRKKQKALLVGINDYPDPAQRLEGCINDV
                     FTMSSVLQDCGVPPEAIRTCLDGRATADGILSRLKWLLDDPKPGDELVFYYSGHGARI
                     PEYGNDYEPDHYTESLVPWDFDWSPEKAISDDQIFNLYSQLPYDCRLLMIFDCCHSGG
                     IHRDGGLRPRGLTPPDDIRHRELKWDSKTEMWVSREFTRINDQFTPKKELAARYFGRD
                     GATQRLGRASMLRGTSSRTYAKLKKEKSVMARGPYLPLIIEACGEDEFSYEYRHGATS
                     HGAFTYSLASILRKQKSITFEELVARTRQQLLDLQYDQEPRILGPEIVVKHKVPWTGG
                     "
BASE COUNT          490 a          666 c          683 g          458 t
ORIGIN      
        1 cccttgcgct ccgtcaaacg cgatttgcgc caagaggaga attgcagttg aaaccagcag
       61 gcgagaaaat gtaacctggt tgggtgtgat cggcgtcgtg tcgagcactt ccttgagtgc
      121 cacgtgggcg atgctccaca gcaggggcac gccgagcgca aacaccgacc agaacgaaaa
      181 gccgtcgatc cgccaggtgc cgctggttgc cagatagtaa agtgctacga tgatgaggaa
      241 cgtgaaaaag agttcgcccc tgctcttcat cctcccgaaa aaaatcgctt ccagaagcat
      301 cgcgaagagc ggataggcct gcagcgcgat ggccgcgctc accgcccctg ccttttcaaa
      361 ggccaggaca taaacatagg tcgaaagccc gaaaatcgct ccggttgtta cggtgatggc
      421 gagtgtccgc gctttcctgg caggagacag ccctgccctg aagatccctt tgttgtttga
      481 acgcaattca cgcgcaaaga gcgggatgga aaagaggagc tgccaaaacg acaaatacaa
      541 ggcaaaggtc agcgcatcgg agcctggcgg ccggccattc gaaatgatcg gcatcacgcc
      601 aagaatgaca aggcagatga aagccagggc gatgcccagg cgcggagaaa gcggagccat
      661 attgtataag gattccggag caatgacgtg tcacatcgtt actctcggtg aaagacgcgc
      721 acaacaaaaa aattgacatc atgacaattt tgaagcgctg ccgcggcgct aaccaccagt
      781 ccacggcacc ttgtgtttca caactatctc cggcccgaga atcctcggct cctgatcata
      841 ttgcaggtca aggagctgtt gccgggttct tgccacgagt tcctcgaagg tgatcgactt
      901 ctgcttccgc aggatgctgg cgaggctgta ggtgaaggcg ccgtggctgg tggcgccgtg
      961 ccggtattcg tatgagaact catcctcgcc gcaggcttca atgatcaggg gcagataggg
     1021 gccccgcgcc atcaccgact tttccttctt cagcttggcg tatgtgcgcg aactggtgcc
     1081 gcgcagcatt gaagcgcgcc ccagccgctg cgtggctccg tcccgcccaa agtaccgggc
     1141 tgccagttcc ttctttggcg tgaactggtc attgatccgc gtaaactccc tgctgaccca
     1201 catttccgtc ttgctgtccc acttgagctc gcgatgccgg atgtcgtcgg gcggggtgag
     1261 cccccgcggc cgcaggccgc cgtcccgatg gatgccgccc gaatggcagc aatcgaaaat
     1321 catcagcagg cggcagtcat aagggagctg actgtaaaga ttgaagatct gatcatccga
     1381 gatggccttc tccggcgacc agtcgaaatc ccatggaacc aggctttcgg tatagtggtc
     1441 gggttcgtaa tcattgccgt attccggaat gcgcgctccg tgcccgctgt agtagaagac
     1501 aagctcgtca ccgggcttgg gatcatcgag cagccatttc aagcggctga gaatgccgtc
     1561 ggcggtggcc cgcccatcca ggcatgtgcg gatggcctcc ggaggaacac cgcaatcctg
     1621 caggacggag ctcatggtga aaacgtcatt gatgcaccct tcaagccgct gcgccggatc
     1681 cgggtagtcg ttgatgccaa cgagcagggc cttctgtttc ttgcggccgc ttccggcggc
     1741 agcgcgggcc tttaccgtgg gggccgagcc gaatgctctg gcgccgactg cctccgctcc
     1801 aatggttctc cagacgtttt caatcgtcga ccggtggctg aaatagcttt cggccgcatg
     1861 gtcagcgatg ccatccacgt caaaaggcgt ctccgtctgc cggaagtttg caccatccca
     1921 gaggcgtatg ggcgccgtga aaaccgagtc gtggatgttg tagagatgat gccagaagct
     1981 gaccgccagc ggcaggacgc ggccattggt aagattccgg atgacgaagg gattggcgat
     2041 ctgtgatccc agcgtgacat atttggcctt cgccagcagg cgcttgacct gctcttcctt
     2101 cgcatccgga tggctgaagg cattgtaggt gacgagcgag ccgaggctat gggcaaggat
     2161 cacgtcgggc cggtgttcgc gcaccgcgtc aaggatgcgc ttgcggctct gctgctgaaa
     2221 cccctcgtct tcgacccagg ccaccacata acccgctgtc catcggatct tgtccgaaat
     2281 gttcgagatc acgccac
//
LOCUS       DAORVN010000343         2295 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_47699_length_2295_cov_3.735714, whole genome shotgun sequence.
ACCESSION   DAORVN010000343 DAORVN010000000
VERSION     DAORVN010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2295
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47699_length_2295_cov_3.735714"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(147..1484)
                     /locus_tag="PKV23_07375"
     CDS             complement(147..1484)
                     /locus_tag="PKV23_07375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85291.1"
                     /translation="MNHPLSRNELSGAENNSENPAETPVPSWLTQHYRDDDAEFLVNQ
                     YRRKQEDEPAAPSAPPENRTYEYYRRRIMERQREQLDQQMERAPMPQVPSRPTLPVSE
                     LDVHHRHYGNERESVERPVSYASTYERPRLRKSLLLAGVFAAVAGGAVGFAVTKYDEI
                     SNSANGLYAFAAGLLPSEPESLFEANRETTISKKPIAIASLEVSDVTGTLNSMIPLAL
                     NAEPAFENQELAIKISGLPASAYLTAGKKISDNTWILKDGEEDGISLFVSQSETAKFD
                     ISVAAIETNTGELAAPVKEMTVAIDDVKLVNETALQITPVSAPPATSIPVKETQPEIV
                     DALPVPVEPFTPIKPALSAEVKGLLDKGDMLMKTGDLIIARQFYSRAFQMGAAEGAMG
                     VAKTYDPAVYTEMKVQGITPDAAKAAEWYEKAKAAGVTEASAALTTPPAAAQP"
     gene            complement(1497..>2295)
                     /locus_tag="PKV23_07380"
     CDS             complement(1497..>2295)
                     /locus_tag="PKV23_07380"
                     /inference="COORDINATES: protein motif:HMM:NF028178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HQX85292.1"
                     /translation="HTDGLRVLPIIGRGSLQSINDLLFLRGVDAALLSSDSLAFVKKH
                     NLYEDETGKLAYLAKLATLNVVVVARKEIAGIDNLAGMRVATGPANSDEFVAAELIFD
                     ALGTTFERVPTTGKNSLAALLDGRIDAAVFVGASSYSLLNTIKANSGLHVLSLSLPET
                     LTETYSPAILENKDFPRLIAEGTVVETVAAALILAVFDWPERSERFYKMRKFNKALLS
                     TYLASRSAEEGTNFSAAVPGWKQYLTAKDLMGTTKAPQQSLTITTLQ"
BASE COUNT          426 a          657 c          699 g          513 t
ORIGIN      
        1 gcacgcaata gaccctttca atgccgtggg cttccaagca tttgacgatc aggtccgcgg
       61 cgcgcatcaa tcttctccgg tagtggttca gaacatcctg atagatgcct tgaacccgcc
      121 gggaagtcga ggccgtttcg ccagggttat ggctgggctg cggcaggcgg ggttgtgagc
      181 gccgctgagg cctccgttac acccgccgct ttggcctttt cgtaccattc cgctgccttg
      241 gccgcgtcag gggtgatgcc ctgcaccttc atctccgtat agaccgccgg gtcataggtc
      301 ttggcgacac ccatcgcccc ttcggccgcg cccatctgga aagcccggct gtagaactgc
      361 ctggcgatga tcaggtcgcc ggttttcatc agcatgtcgc ccttgtccag aagtcccttc
      421 acttccgcag acagcgccgg cttgatgggc gtgaagggtt ccaccggaac cgggagagca
      481 tcaacaatct ccggctgcgt ttccttgacc gggatgctgg ttgctggcgg ggcgctgacc
      541 ggggtaatct gcagcgcggt ttcattcaca agcttcacgt catcgatggc gaccgtcatt
      601 tccttcaccg gagcggccaa ttcacccgtg ttggtttcga tggccgcaac tgaaatgtcg
      661 aattttgccg tctcggattg cgacacgaag agactgatgc cgtcttcctc gccgtccttc
      721 aggatccagg tattgtcgga aatcttcttt cccgccgtca ggtaggcgga ggcgggaagc
      781 ccggaaatct tgatggccag ctcctggttt tcaaacgccg gttcggcatt aagcgccagg
      841 gggatcatgc tgttgagggt gcccgtgaca tcagatacct caagggaagc gatggcgatg
      901 ggctttttgg agatcgtcgt ttcgcggttt gcttcaaaca gcgactctgg ttcgctgggc
      961 agcaggccgg cagcgaacgc gtaaagtcca ttggccgaat tcgaaatctc gtcatacttc
     1021 gtgacggcaa atccgacagc tccgccggcc acggccgcaa agacccctgc cagcagcagg
     1081 ctcttcctga gcctcggccg ttcataggtg gaggcgtagg aaaccggccg ttcaaccgat
     1141 tcccgttcat tgccataatg gcgatgatgc acgtccagtt cactcacagg aagtgttggc
     1201 cgcgacggaa cctgcggcat gggggcccgt tccatctgct ggtccagctg ttcgcgctgc
     1261 cgctccatga tccggcggcg gtaatactca taagtccggt tttccggggg cgccgacgga
     1321 gcagccggct catcctcctg cttgcggcgg tactggttga ccagaaactc tgcgtcatcg
     1381 tcgcgataat gctgcgtcag ccatgaggga accggggttt ccgctgggtt ttccgagttg
     1441 ttttctgcgc cgctgagttc attccgcgaa aggggatggt tcatggtcga atcctcctac
     1501 tgtaacgtcg taattgtgag tgattgttgg ggcgctttgg tggtgcccat gaggtctttt
     1561 gcggtcaggt actgtttcca ccctggaact gcggcagaga aattggtgcc ttcctcagcg
     1621 ctgcggctgg cgagataggt ggaaagcagg gccttgttga atttcctcat cttgtaaaac
     1681 cgctcgctgc gctcaggcca gtcgaagacg gccaggatca aggccgccgc gaccgtttcc
     1741 acaacagtgc cttcggcgat gaggcgcgga aaatccttgt tttcaagaat tgccggcgaa
     1801 taggtctccg tcaaagtctc aggcagcgag agggaaagaa cgtgcagccc actattggcc
     1861 ttgattgtat tcagcaggct gtaggaggag gcgcccacga atacggccgc gtcgatgcgg
     1921 ccatcaagca atgccgccag cgagttcttc ccggtcgttg gaacccgctc gaaagtggtt
     1981 cccagcgcat cgaaaatgag ttcagcggcc acgaactcgt cgctgttggc agggcccgtc
     2041 gccacccgca tgccagccag gttgtcaata ccggcgattt ccttgcgcgc caccacaacg
     2101 acgttcaggg tcgcgagctt tgccagatag gcaagtttgc cggtctcgtc ttcgtagaga
     2161 ttgtgcttct tgacaaaggc aaggctgtcg gaggagagca gcgcggcatc gacgccgcgc
     2221 aggaagagaa ggtcattgat cgattggagg ctgccacgtc cgatgatggg gaggacccgc
     2281 aagccatctg tgtgg
//
LOCUS       DAORVN010000344         2292 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_47840_length_2292_cov_1.991954, whole genome shotgun sequence.
ACCESSION   DAORVN010000344 DAORVN010000000
VERSION     DAORVN010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2292
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47840_length_2292_cov_1.991954"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..835
                     /locus_tag="PKV23_07385"
     CDS             <1..835
                     /locus_tag="PKV23_07385"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HQX85293.1"
                     /translation="YAFPWIGSRPIAEVTAPELLAIIRRVKDMGYLDTAHRLRMTAGQ
                     VFRYGISTGRCERDISADLRGAIPPQRKKHYAAITDPAEFGRLLRDIWAYQGSLITCS
                     AFRLSALFGLRPGEVRNLEWSEVDLDAKLIRIPLGKMKARRLHVVPLADQAALILEEL
                     KPLTGAGRFCFPGLQSRDRPMSENTINAALRRLGYDTQSEQSAHGFRSSFSTMAHGSG
                     LFRPEVVEVQLAHKHGDAVRLAYDRGDFLEERRKLMAWWAGQCDQMRNGAEIIPLGAV
                     A"
     gene            835..1470
                     /locus_tag="PKV23_07390"
     CDS             835..1470
                     /locus_tag="PKV23_07390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85294.1"
                     /translation="MKIKLKGMDGDIKAMVESVAYAAELHRLHPDLAAVPAFSPEAQE
                     IAQLFVAGFPKPEQIPNPPGGWPEYFQFMAHEAVNVNDRRHRDEMRDRVCTTLLHGGA
                     LNVAERAWLIYVLSKLKELPHESKGRAIKGNERIEFMLQLIGFIEYEERKAGEYKPGD
                     VTKRLHDAAEHFGKGFETVNAMYKSKAFKRLRKNLAERGNIERALFTKTTI"
     gene            1543..1773
                     /locus_tag="PKV23_07395"
     CDS             1543..1773
                     /locus_tag="PKV23_07395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85295.1"
                     /translation="MARIIIPPALAAFDSRPDSALIDVKVFSGVFGCSENTVWRRYGA
                     LAIKVSPQQTRWRVADVRKALADLSKPGQAAA"
     gene            2056..>2292
                     /locus_tag="PKV23_07400"
     CDS             2056..>2292
                     /locus_tag="PKV23_07400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85296.1"
                     /translation="MADTKTRGAGERQPSQLSRINPLIDQHGDPVHVQTVLGLLSFLI
                     LDATEKSITLSDDDAHGLALILETCKAALANLPRN"
BASE COUNT          474 a          652 c          682 g          484 t
ORIGIN      
        1 ctatgccttt ccctggatag ggagtcgacc gattgccgag gtcacggcgc ccgagctgct
       61 ggcgatcatt cggcgagtca aggacatggg ttatctcgac acagcacacc gcctgcgaat
      121 gactgccggg caggtgttcc gctatggcat cagtaccggc cgctgcgaac gcgacatatc
      181 ggccgacctg cgcggcgcca tcccgccaca acggaagaaa cactacgcgg cgattaccga
      241 tcctgcagag ttcggcaggt tgctgcgcga catctgggcc taccagggca gcctgattac
      301 ctgttccgcg ttccggctct ctgcgctgtt cggcctgcgt cctggcgagg tccgcaacct
      361 ggaatggtcc gaggttgatc ttgatgccaa gttgatccgc atccccctgg gcaagatgaa
      421 ggcgcgacgg ttgcatgtcg ttccattggc cgaccaggcg gcgctgatcc tggaggaatt
      481 gaagcccttg accggggcag ggcggttctg ttttccgggg ctgcagagcc gtgatcggcc
      541 catgagcgag aacaccatca acgcggcgct gcgccgcctg ggctacgaca cccaaagcga
      601 gcagtcggcg cacggtttcc ggagttcgtt ttccacgatg gcgcatgggt ccggtctgtt
      661 ccggccggag gtcgtcgagg tgcagctcgc ccacaagcac ggcgacgcgg tgcgcctggc
      721 ctatgaccgg ggcgacttcc ttgaggagcg gcgcaagctt atggcctggt gggctggcca
      781 gtgcgaccag atgcggaatg gtgccgagat cattccactt ggagctgttg cgtgatgaag
      841 atcaagctta aaggcatgga cggcgatatc aaggccatgg tcgagtcggt tgcgtatgcg
      901 gccgaacttc accgattgca tccggacctg gccgctgttc ccgcgttttc gcctgaagct
      961 caggaaattg cgcagctatt tgttgctggt tttccaaagc cagagcaaat acccaaccca
     1021 ccaggtggat ggcctgagta ttttcagttc atggcgcatg aagctgtcaa cgtgaatgac
     1081 aggcgtcacc gggatgaaat gcgagatcgt gtgtgcacca cattgctgca tggtggcgct
     1141 ctcaatgttg ctgagagggc ctggctgatc tacgttttgt ccaaacttaa ggaactgccc
     1201 cacgaatcaa aagggcgggc gatcaaaggt aatgaacgga ttgagttcat gctgcagctc
     1261 ataggcttca ttgagtatga agaacgtaaa gccggcgaat acaagcctgg ggatgtaacg
     1321 aaaaggctgc acgacgcagc agagcacttc ggcaaaggct ttgagacggt gaacgccatg
     1381 tacaaatcga aggcgtttaa gcggctgcgc aaaaatctcg ccgaaagggg aaatattgaa
     1441 cgtgcattgt ttaccaagac aacaatttag cgcgctccta cattgccgcc atgcacccag
     1501 cgacaccatc ccggcgtcgt gggttcctac caaggaaaag ccatggcccg catcattatc
     1561 ccgcccgcat tggcggcgtt tgactctcgc cctgattcag ccctgatcga tgtcaaggtg
     1621 ttttccggtg tcttcggatg ctcggaaaac actgtctggc gccgctatgg cgccctggcc
     1681 atcaaggtat ccccgcagca gacccgctgg cgtgtcgccg atgtgcgaaa ggcgctggct
     1741 gacttgagta agcccgggca ggcggcggca tgaaaactgc gcttgaccag tccggtcgac
     1801 cgtggcaatc tcggtccccg gtgctcaaca cacctcaact agcggtatcc gctcccgtca
     1861 gtctcagcgg attttttacg tccataggtt tcgtccgtgt cggcttcttg ccgtcatggc
     1921 ttgctcaatg gtcgggtagt gcgcagtcat gcaatagcct tcgggtaaaa gctgcgagcg
     1981 gtctagttgc cgtgttgaag tacctgacca cccctcaaca gggtggctat tcaacgaacc
     2041 aactaggagg ccatcatggc tgataccaaa acccggggcg cgggcgaacg ccaaccttcc
     2101 cagctttctc gcatcaaccc gctgatcgat cagcacggcg atccggtaca tgtccagact
     2161 gtcctggggc tactgtcgtt tctgattctg gacgcaacag agaagagcat cactctttcg
     2221 gatgacgatg cgcatggttt ggcgctaatc cttgagacct gtaaggctgc gctcgcgaat
     2281 ttgccaagaa at
//
LOCUS       DAORVN010000345         2291 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_47863_length_2291_cov_3.199016, whole genome shotgun sequence.
ACCESSION   DAORVN010000345 DAORVN010000000
VERSION     DAORVN010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2291
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47863_length_2291_cov_3.199016"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1315)
                     /gene="gndA"
                     /locus_tag="PKV23_07405"
     CDS             complement(<1..1315)
                     /gene="gndA"
                     /locus_tag="PKV23_07405"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012708320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HQX85297.1"
                     /translation="MSQAEIGLIGLGVMGSNLALNIAEKGNRIAVFNRTPERTDAFMA
                     SAGNFAADLVPAKTMEDFIASIRPPRPVIIMVKAGEAVDEQIAALKKLMAKNDIVIDA
                     GNANFRDTMRRFQDLDGSGLTFIGMGVSGGEEGARHGPSIMVGGTPESYARVEKVLSA
                     IAAKFQGEPCCAWLGPNGAGHFVKTIHNGIEYADMQMIAEIYGVLRDGLGMRPKAIGE
                     VFARWNKGRLNSYLIEITAAVLAANDPKTGAPMVDVILDSAGQKGTGRWAVIEAQMMG
                     VPATAIEAAVAGRSLSAMKKERVAASAVFTKAGSNSLGDPNSLIDKLELALFAGKIAA
                     YAQGFAVMEAASREFTWNLPMATIARIWRAGCIIRSQFLDTIASAFEQNKGLSNLLLA
                     PDFVTMMQGATPALRHIVARGAQEGLPLPALSSALGMVLSQVVQLG"
     gene            complement(1326..1841)
                     /locus_tag="PKV23_07410"
     CDS             complement(1326..1841)
                     /locus_tag="PKV23_07410"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015915018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HQX85298.1"
                     /translation="MFKGAVIVMGVTSCGKTSVGEGLAAALDCPFIEGDRLHPASNIV
                     KMSAGTPLTDADRWPWLEIIGKAMKAEYDKGHGVVASCSALKKVYRQKLAEAVGQPIT
                     FVHLYGSRDLLAARMAERKGHFMPPSLLDSQLATIEVPGPDETALHLDVILPVDALVR
                     RSKAYLTSNKD"
     gene            1959..>2291
                     /locus_tag="PKV23_07415"
     CDS             1959..>2291
                     /locus_tag="PKV23_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008529987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HQX85299.1"
                     /translation="MKAIVIHGALDLRVEERAPATPGPEEVSIAIRRGGICGSDLHYY
                     QHGGFGTIRLKEPMILGHEIAGEVAAIGAKVTGLKVGDRVAVNPSRPCKVCAYCQAGM
                     QNHCLDMLF"
BASE COUNT          430 a          681 c          733 g          447 t
ORIGIN      
        1 agccgagctg caccacctgg gagagcacca tccccagcgc cgaggagagc gccggaagcg
       61 gcaggccttc ctgggcgccg cgggccacga tatggcgaag tgccggggtg gcgccctgca
      121 tcatggtgac gaaatcgggg gcgagcagaa gattgctcag gcccttgttc tgttcaaagg
      181 ccgaggcgat ggtatcgagg aattgcgagc ggatgatgca gccggcgcgc cagatgcggg
      241 cgatggtggc catgggcagg ttccaggtga attccctcga agccgcttcc atcaccgcaa
      301 agccttgcgc ataggcggcg atttttccgg caaagagcgc aagctccagc ttgtcgatca
      361 gggaattggg atcgcccaat gaatttgagc cagcttttgt aaatacggcc gaggcggcca
      421 cgcgttcctt tttcatggcg gagaggctgc ggccggccac ggccgcttca atggcggttg
      481 ccggaacgcc catcatctgc gcctcgatca cggcccagcg gccggtgcct ttctgcccgg
      541 cggaatcgag gatcacatcg accatcggcg caccggtctt tgggtcattg gcggccagca
      601 ctgcggcggt gatttcaatg agataggaat ttagccggcc cttgttccat ctggcaaaga
      661 cttcgccaat ggccttgggc ctcatcccca gcccgtcgcg cagcacgcca tagatttcgg
      721 cgatcatctg catgtcagca tattcaatgc cgttgtgaat ggttttcacg aaatggcccg
      781 caccattggg gccgagccag gcgcagcagg gttcgccctg gaatttggcg gcgatggcac
      841 tgagaacctt ttcaacccgc gcataggact cgggcgtgcc gcccaccatg atggaggggc
      901 cgtggcgcgc accctcctcg ccgcccgata cgcccatgcc gatgaaggtc aggccgctgc
      961 cgtcaagatc ctggaagcgg cgcatggtgt cgcggaaatt ggcgttgccg gcgtcgatga
     1021 caatgtcatt tttcgccatc agctttttca gggcggcgat ctgctcgtcc acggcttcgc
     1081 cggccttcac catgataatc accgggcgcg gcgggcggat gctggcaatg aagtcctcca
     1141 tggtttttgc gggaacgagg tcggcggcaa aatttccggc cgaggccatg aaggcatcgg
     1201 tccgctcggg ggtgcggttg aacacggcga tgcggttgcc cttctccgcg atgttcagcg
     1261 caagattgga gcccatgacg ccaaggccga tgaggccgat ttcagcttgt gacatgttca
     1321 ggtcctcaat ccttgttact ggtcaaatag gctttagagc gtctcaccaa cgcgtcaact
     1381 ggcaggatca cgtcgagatg aagcgctgtc tcgtcggggc ccggcacttc aatggtcgca
     1441 agctggctgt cgagcaatga agggggcata aaatggccct tgcgctcggc catgcgcgcc
     1501 gccagcaggt cgcggctgcc atataagtgg acgaaggtta tgggctgccc cacggcctca
     1561 gcgagttttt gccggtagac tttcttcagc gccgagcagg aggcgaccac gccgtgcccc
     1621 ttgtcgtact cagctttcat ggccttgccg atgatctcga gccagggcca gcggtcggcg
     1681 tcagtcaggg gcgttccggc ggacatcttt acaatgttgg aggcagggtg cagcctgtcg
     1741 ccctcgatga agggacagtc caacgccgcg gccaggccct cgcccacgct ggtcttgccg
     1801 caactcgtca cccccatgac aatgaccgcg cccttgaaca ttgacccaca acactcctgc
     1861 cagatcggtt agttgccata agttttgcac tggcgccatg ggcagggcac gggcacagtg
     1921 gcgcttaaaa ggggtttcga acaggcagca atatgggcat gaaagcaatc gtcattcatg
     1981 gggcgctcga cctccgcgtg gaggagcgag cgcccgcgac gccggggccg gaggaggttt
     2041 ccattgccat caggcgcggc ggcatttgcg gatcggacct gcattactac cagcatggcg
     2101 gattcggcac gatccgcctg aaggagccga tgattctcgg ccatgagatt gccggcgagg
     2161 ttgcagcaat tggggccaag gtcaccggtt tgaaagtcgg agaccgggtt gcggtcaacc
     2221 ccagccgccc gtgcaaggtt tgcgcttact gccaggccgg gatgcagaac cactgcctcg
     2281 acatgctgtt c
//
LOCUS       DAORVN010000346         2290 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_47903_length_2290_cov_3.069351, whole genome shotgun sequence.
ACCESSION   DAORVN010000346 DAORVN010000000
VERSION     DAORVN010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2290)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2290)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2290
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47903_length_2290_cov_3.069351"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(178..543)
                     /locus_tag="PKV23_07420"
     CDS             complement(178..543)
                     /locus_tag="PKV23_07420"
                     /inference="COORDINATES: protein motif:HMM:NF018008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HQX85300.1"
                     /translation="MPEARLFKRAELPFKDCVTPSGVTQTARTITTSISKQMGGGMEI
                     GEDVCIHWTTLYDEILFIHKGSMIVRTSAGELECRPGDIVWLSEGVTLDYDMTGRRCE
                     YFYALFPIDWAKRNNMEEP"
     gene            complement(546..1970)
                     /locus_tag="PKV23_07425"
     CDS             complement(546..1970)
                     /locus_tag="PKV23_07425"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQX85301.1"
                     /translation="MTTPTRRDGRCLWLRQAIQPGEVDAPVLAGNLTADICVVGGGLA
                     GLWTALDLKERDPSCDVAIVEADICGGGASGRNSGMVLSQWAKFAALRAFCGEAGAIA
                     LGHAFAASAGNIERFCKAHGIDAEFRPDGWIWGATCEKHVGSWNGILQNLEAIGLHPF
                     KRVTGPEIRALCGTDSFLAGIHDPTAATLHPGKLVRGLRRVALAKGVRIFENSRMLKL
                     ERQSPAIVRTAKGTMRSPVCILTLNAWSTSVPELRPSILVIASDDSVTEPVPELLEKM
                     GYLRRPLMGDSQTFVTGFRTTADHRFQPGITGGIIGFGGLGTNRFEGRSIREADIRSC
                     ISRGFPAFADLPFADSWCGPIDRTRSGLPLFGPLPGSPNIFYGFGFSGNGVATTPVTA
                     KILASLALGKKDEWSSCGLVRPPERWLPPEPIRYIGAHMVRHAIRRKDRLEQENRVPG
                     PLVRALAAMAPGGITTSTIISPRR"
     gene            complement(1951..>2290)
                     /locus_tag="PKV23_07430"
     CDS             complement(1951..>2290)
                     /locus_tag="PKV23_07430"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HQX85302.1"
                     /translation="LFDGATFHYLALMGGPRHLKESQRGWVDAKQTIEYRREILAGDL
                     LVISTAMTRVGRSSLGFRHELVDLEDGALRATSDHVVVHFDLSKRASLTLSEAMVEKA
                     REYLNDDTNA"
BASE COUNT          481 a          661 c          670 g          478 t
ORIGIN      
        1 gttccagcgc ggagaaagtt aaaaatcagt ggaaactgat gcaggttggt tgactggtca
       61 tagtgctgac gcatggctgt atccgaatgg cgatcccgac taggagtgcg taacccggca
      121 cttttttgcc gaaggttcat cggagtattg ctgattcaca aatgtgatgc gggagcgcta
      181 aggctcttcc atgttgtttc tttttgccca gtcgattggg aaaagggcat aaaaatactc
      241 gcaccgtctt cctgtcatgt catagtcaag ggtaactcct tcggacagcc atacgatatc
      301 cccggggcgg cattccagtt cgccagcgct ggtgcgcacg atcatcgagc ccttgtggat
      361 aaacaggatt tcgtcataga gcgtggtcca gtgaatgcag acatcctctc cgatctccat
      421 gccgccgccc atctgcttgc tgatgctggt ggtaatggtc cgggcggtct gcgtcacgcc
      481 tgatggtgta acgcagtcct tgaagggaag ttcggcgcgc ttgaacagtc tggcttcggg
      541 catgattacc tcctcgggct gatgatggta gatgtggtga tgccacccgg ggccatggcg
      601 gccaaggcgc ggaccaaggg gccgggaacg cggttttcct gttcgagccg gtctttgcgg
      661 cggatcgcgt gacgcaccat atgggcgccg atgtatcgaa tgggttctgg tggaagccag
      721 cgctcgggcg gtcgcactag tccacaagat gaccattcat cttttttgcc aagggcaagg
      781 cttgcaagaa ttttggctgt caccggggtt gtcgccacgc catttccgga aaagccaaat
      841 ccataaaata tatttggtga acccggcagg ggaccaaaaa gcggcaagcc gctgcgcgta
      901 cgatcgatcg gcccgcacca gctatcggca aaaggcaaat cggcgaaagc cgggaagccg
      961 cgactgatgc aactccggat gtcggcttca cgtatggagc gcccttcaaa ccggttggtg
     1021 ccgagaccgc caaatccgat tatgccgccg gtgatcccgg gctgaaagcg gtgatcagcg
     1081 gtggttctga atccggtcac aaaggtttga ctgtctccca tcaggggacg ccgcaggtaa
     1141 cccatttttt ccaggagctc ggggacaggc tcggttacgg aatcatcgct ggcaatcacc
     1201 aggattgagg gacgaagctc gggcaccgac gttgaccacg cattgagtgt aaggatgcaa
     1261 accggtgacc gcatggttcc ttttgccgtg cgaacaatcg ccggactttg ccgctcgagc
     1321 ttcagcattc gcgaattctc gaaaatgcga acgcccttgg ctaaagccac gcggcgcagg
     1381 ccgcgcacca gtttgccggg atgaagcgtt gcggccgtgg gatcatggat gcctgcaagg
     1441 aaactgtcgg tgccgcaaag cgccctgatc tccggccctg tcacacgctt aaaaggatgc
     1501 agcccaatcg cctccaggtt ctgcagaatc ccattccaag atcccacatg tttttcgcag
     1561 gtggcgcccc aaatccagcc gtcagggcgg aactccgcat caattccatg ggccttgcag
     1621 aagcgttcga tgtttcccgc ggatgcggca aaggcgtggc caagggcgat ggcgcctgcc
     1681 tcaccgcaga aggctctcaa tgccgcaaac ttggcccact gagacaacac catgccagag
     1741 ttccgccctg acgccccgcc gccgcagata tcggcttcaa caattgccac atcgcaggaa
     1801 ggatcacgtt ccttgaggtc aagcgcggtc cacaggccgg ccaggccgcc gcccacgacg
     1861 caaatgtcag cagttaggtt tcctgcgaga acgggcgcgt ccacctcgcc aggctggatc
     1921 gcctgacgca gccagaggca tcggccgtct ctacgcgttg gtgtcgtcat tgagatactc
     1981 tcttgccttt tctaccattg cctcggaaag cgtgagggac gcacgcttac tgagatcgaa
     2041 atgaaccacc acgtgatcgc tggttgcgcg caaggcgccg tcttccagat caaccagttc
     2101 atggcgaaag ccaagcgacg agcggccgac acgggtcatt gctgtggata tcaccagcag
     2161 atcgcccgcc aggatttccc gtcggtattc gatggtctgc tttgcgtcta cccagccgcg
     2221 ctggctttcc ttcaagtgcc gtggcccgcc cattagcgcc agatagtgga acgtcgcgcc
     2281 atcgaacagt
//
LOCUS       DAORVN010000347         2289 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_47932_length_2289_cov_3.655774, whole genome shotgun sequence.
ACCESSION   DAORVN010000347 DAORVN010000000
VERSION     DAORVN010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47932_length_2289_cov_3.655774"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..674)
                     /locus_tag="PKV23_07435"
     CDS             complement(<1..674)
                     /locus_tag="PKV23_07435"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline/ethanolamine kinase family protein"
                     /protein_id="HQX85303.1"
                     /translation="MAAEDRVRKLPCWTGPIEVVPLKGGVSNASFTVQDSAGKFVARV
                     GEDYPFHHVFREAEVIASRAAFEAGLSPEVIHAEPGIMVVRFLAARTYGEADVRENAE
                     ACVDIVKRCHAEMGKRISGRANFFWVFHVLRDYAHTITAAKHRHVPDIPRWLAEVERL
                     EKAQVPLPIIFGHHDLLPTNFMDDGKRLWLIDWEYGGFGTAMFDLANIASNNQFGEQA
                     EKDLLTR"
     gene            825..1595
                     /locus_tag="PKV23_07440"
     CDS             825..1595
                     /locus_tag="PKV23_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013528029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HQX85304.1"
                     /translation="MPAALSKRQTQILSLVREQGTCSVADLAKALDVSFETVRRDIKP
                     LVDSREVSRRHGSIALPHELGEAPFERRLREHFAAKRAIARRAARLIEDGDSLMLDTG
                     TTTSIFARELLKKRNLTVVTNSSDIARTLATVNGNRVYMAGGELNGSNGAAFGPSAIA
                     FAASFHVRHAIISIGALDADFGPSDSYLAEAEFARMVLSRGETRVMLSDASKFGRTAL
                     IKVCDFKDINRLITDGIPDAKLAAVLQEAGVSVNVVAH"
     gene            1703..>2289
                     /locus_tag="PKV23_07445"
     CDS             1703..>2289
                     /locus_tag="PKV23_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010968071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HQX85305.1"
                     /translation="MRYEAPETLKSAVTLLAKEKGQAFVLAGGTDLLVKMKGGFLEPD
                     LVVDIKRIKGVFDIAKSATGFRIGAAVPGVQLGENAALVKAWPGVVEGANLIGSKQVQ
                     GRCTMVGNLCNGSPAADGVPSLIAANAKVVISGPKGKRTIPVEQVPVSPGRTSLKKGE
                     IIEAILLPKRPANSSDAYLRFIPRTEMDIAVVSAAV"
BASE COUNT          489 a          747 c          610 g          443 t
ORIGIN      
        1 cgcgtcagca gatccttctc cgcctgttcg ccgaactggt tgttggaggc gatattggcc
       61 aaatcgaaca tcgccgtgcc gaagccgcca tattcccagt caatcagcca cagccgtttt
      121 ccgtcatcca tgaaatttgt cggcagcagg tcgtgatggc cgaaaatgat cggcagcggc
      181 acctgtgctt tttccaggcg ctcaacttcc gccaaccagc gcggaatgtc cggcacatgg
      241 cggtgcttcg ccgccgtgat ggtgtgggca tagtcgcgca gcacatgaaa tacccagaag
      301 aaattggccc gtcccgaaat gcgcttgccc atttccgcgt ggcagcgctt cacgatgtcg
      361 acgcaagcct ccgcattttc ccgcacatcc gcttccccat atgtgcgcgc cgcgagaaag
      421 cgcaccacca tgatgccggg ctccgcatga attacctccg gcgacagtcc ggcctcaaaa
      481 gcggcccggc tggcgataac ttccgcctcg cggaacacat gatggaacgg atagtcctcg
      541 cccacccggg ctacaaattt gcccgcgctg tcctgaaccg taaaactggc gttgctcacg
      601 ccccccttca gcggaaccac ctctataggt cccgtccagc atggcagttt ccgaaccctg
      661 tcttctgctg ccattggcca tctcctcacc tgaaatgaag cctttcagag ccaacaggtc
      721 aacaataatg ttgttttatg tggcaattat gcattatttg tgtggtaatt tgactgaaac
      781 gttcaataga ttgccaaaag ccacaaatcg tggtcaattg caccatgcca gcggcactca
      841 gcaagcgcca aacccagatt ctcagcctcg tccgcgaaca gggaacctgc tctgtcgccg
      901 atttggccaa agccctggac gtttcctttg aaaccgtccg ccgtgacatc aaacccctgg
      961 tagactcccg cgaggtctcc aggcgccacg gctccatcgc ccttccccac gaactgggcg
     1021 aagcgccctt cgagcgccgc ctgcgcgaac atttcgccgc caagcgcgcc attgcccgcc
     1081 gcgccgcccg gctgatcgag gatggcgaca gcctgatgct cgacaccggc accaccacca
     1141 gcattttcgc ccgcgagctc ctgaaaaaac gcaacctcac cgtcgtcacg aattcgtcag
     1201 atatcgcccg cactctggca accgtgaacg gcaaccgcgt ttacatggca ggcggcgaat
     1261 tgaacggctc caatggcgcg gcctttggcc cctcggccat cgctttcgcc gctagcttcc
     1321 atgtccgcca cgccatcatc tcgattggcg cgctggatgc ggacttcggc ccctccgaca
     1381 gctatctcgc cgaagccgaa tttgcccgca tggtgctgtc gcgcggcgag acccgcgtca
     1441 tgctgtccga tgcctccaaa ttcggccgca ccgcgctgat caaggtgtgc gacttcaagg
     1501 acatcaatcg cctcattacc gatggcattc ccgatgctaa gctcgccgcc gtgctgcagg
     1561 aggcaggtgt cagcgttaat gttgtggcac actgataaaa gccgcagggc agccatgaat
     1621 tatggataga agcgaatatg ccggttgacc aaaacgcacg gaacaggcca aactggcgcg
     1681 ggaactcaaa aacaggtgaa taatgcgata cgaagcaccg gaaacactta aatcggcggt
     1741 cacgctgctg gccaaggaaa agggccaggc ctttgttctg gcgggaggaa cggacctgct
     1801 ggtcaagatg aaaggcggct ttctcgagcc cgaccttgtc gtcgatatca agcgcatcaa
     1861 gggtgtcttt gacatcgcga aatccgccac cggtttcagg atcggcgccg ccgtaccagg
     1921 cgtgcaactc ggcgaaaatg ccgccctcgt aaaagcctgg cccggcgttg tcgaaggggc
     1981 aaacctcatc ggctccaagc aggtgcaggg ccgctgcacc atggtcggca atctgtgcaa
     2041 cggctcgccc gctgccgatg gcgtgccctc cctcatcgcc gcaaacgcaa aagtcgtgat
     2101 ctccggcccc aagggcaagc gcaccattcc ggtggaacag gtaccggtat cgccgggccg
     2161 aacttcgctg aagaagggcg agattatcga agccatcctg cttccaaagc gcccggcaaa
     2221 ttccagcgat gcctatctgc gctttattcc gcgcaccgaa atggatatcg ccgtggtcag
     2281 cgccgccgt
//
LOCUS       DAORVN010000348         2289 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_47936_length_2289_cov_3.425694, whole genome shotgun sequence.
ACCESSION   DAORVN010000348 DAORVN010000000
VERSION     DAORVN010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47936_length_2289_cov_3.425694"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="PKV23_07450"
     CDS             complement(<1..203)
                     /locus_tag="PKV23_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015688987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HQX85306.1"
                     /translation="MVDLAVLDLAPVSEGKTPADAFRSARDLARHVERLGYKRYWLAE
                     HHNMVGIASAATSVVIGYIAGST"
     gene            308..1285
                     /locus_tag="PKV23_07455"
     CDS             308..1285
                     /locus_tag="PKV23_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020629025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HQX85307.1"
                     /translation="MKIDPIEKVALGKTGLSVSRLCFGLGPIGDMPDTYGYKVDAERG
                     RETVRAVFDSPVNFLDTSRNYGFGRSEERIGEVIRERGGIPEGFVISSKLDRDMETTK
                     FDGARARRSLEESLKTLNVSKIQLLHVHDPEYASSLSDVTGPKGALAELFRMKEEGLA
                     DAVGLAAGRVDIMLPLLKDWDFDALITHNRFTLVNRNAEEMMDYATAKGIAVLNAAPY
                     AGGVLAKGSATHKRYVYQVASAETLEPVARVERICAKHGIPPGAAALQFSMRDPRVAS
                     TVCGVSKPERITQTIEWARFPIPQAAWDELMALPYEAGDPEATRVYSPG"
     gene            1333..>2289
                     /locus_tag="PKV23_07460"
     CDS             1333..>2289
                     /locus_tag="PKV23_07460"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HQX85308.1"
                     /translation="MPAFASQLMNEIRERFAHVDTCPFDGPRVFFENAGGALTLKSVA
                     ETSAAYAAYPDNQGRDNPASRALMAAIAKGKADMRNFFNAPTGQVFVGESGTEVLFRL
                     IRTAALGAKSGGIMLSSTLEHPASRSAMTRWAEVTGRPHVLVPHDNKTGSVDAAAYRN
                     QITSEVRVATIIQTSPVTGISVDVKAIAAAIREKSPDCFIVVDGIQHASHGHIDIEAY
                     DVDGYAVSPYKVFSRHGYGVGWASDRLTALPKEGVIGSPPTNWELGTRDAGSYATFSD
                     VVEYFVWLGGRFTKTTDRRARIEAAAKAIHDHEQQLRHAMAHG"
BASE COUNT          462 a          756 c          648 g          423 t
ORIGIN      
        1 ctggtgctgc cggcgatata gccgatcacc accgaggtgg ccgcactggc gatgccaacc
       61 atgttgtgat gctcggccag ccagtagcgc ttgtagccca ggcgctccac atgccgggcc
      121 aggtcgcgcg cgctcctgaa agcatcggca ggcgtctttc cctctgaaac cggggccaaa
      181 tcaagaaccg ctaggtcgac catgtcgttt tcactcaact ttcaagatac actcaggtaa
      241 agcctgtcct aacgcaagat ggtgtgccgt ggaccggaat gttaggtaga agagtgcggg
      301 agagaaaatg aagattgatc caattgaaaa agtggccctc ggaaaaaccg gcctgtccgt
      361 cagccggctg tgtttcggcc tcgggccaat aggcgatatg cccgatacct atggctacaa
      421 ggtggacgct gagcgcggcc gcgaaaccgt gcgcgccgtt ttcgacagcc cggtcaattt
      481 tctcgatacc tcgcgcaatt atggtttcgg ccgcagcgag gagcgcatcg gcgaagtgat
      541 ccgcgagcgc ggcggaattc ccgagggctt cgtcatttcc agcaagcttg accgcgacat
      601 ggagaccacc aagttcgatg gcgcaagggc gcgccgttca ctcgaggaaa gcctcaagac
      661 gctcaatgtc agcaagatac agctcctcca tgtgcatgat cccgaatacg cctcgtcgct
      721 ctccgatgtc accggcccga aaggcgcgct tgccgaactc ttccgcatga aggaggaagg
      781 cctggccgat gcggtggggc tcgccgccgg ccgcgtcgac atcatgctgc cgcttctcaa
      841 ggattgggac ttcgatgcgc tgatcaccca caaccgcttc accctcgtca accgcaatgc
      901 cgaggagatg atggactacg cgacagccaa gggcatcgcc gttctcaacg ccgcccccta
      961 tgccggcggc gtccttgcca agggctcggc gacgcacaag cgctatgtct atcaggtggc
     1021 ctcggccgaa acccttgagc cggttgcccg ggtggaacgc atctgcgcca agcatggcat
     1081 tccgcccggc gccgccgctt tgcagttttc catgcgcgac ccgcgcgtgg catccaccgt
     1141 ctgcggcgtc agcaaacccg aacgcatcac ccagaccatc gaatgggctc gcttccccat
     1201 tccgcaagcc gcctgggatg agctcatggc ccttccctac gaagcaggcg atcccgaggc
     1261 gacacgggtt tacagcccgg gctgaaactt ccctcgccgc tctttccatc aacacccaat
     1321 cagagaaatc tcatgcccgc ctttgcttcc caactgatga acgaaatccg cgagcgtttc
     1381 gcccatgtcg acacctgccc ctttgacggc ccgcgcgtgt tctttgagaa tgccggcggc
     1441 gccctgacgc tgaaatccgt ggcggaaacc tccgccgcct atgccgccta tcccgacaac
     1501 cagggccgcg acaacccggc ttcccgcgct ttgatggcgg ccatcgccaa gggcaaggcc
     1561 gacatgcgca acttcttcaa tgcgcccaca ggccaggtct tcgtcggcga aagcggaacc
     1621 gaggtgctgt tccgcttgat caggaccgcg gcgctgggcg cgaaatccgg cggcatcatg
     1681 ttgagcagca cgctcgaaca tcccgcctcg cgcagcgcca tgacgcgctg ggccgaagtg
     1741 accggcaggc cccatgttct ggtgccccat gacaataaaa caggaagcgt cgatgccgct
     1801 gcctaccgta atcaaattac atccgaggtt cgcgttgcga ccatcatcca gacctcgccg
     1861 gtcaccggca tttcggttga tgtcaaagcc attgccgccg ccatccgcga aaaatcgccg
     1921 gactgcttca tcgtagttga cggcatccag catgccagcc acggccacat tgatatcgaa
     1981 gcctatgatg tcgatggcta tgccgtctct ccttacaagg tgttttcccg ccatggctac
     2041 ggcgttggct gggcttcgga ccgcctgacc gcccttccca aggaaggcgt catcggcagc
     2101 ccgcccacca attgggaact gggcacccgt gatgccggca gctacgccac cttttccgat
     2161 gtggttgaat acttcgtctg gctcggcgga cgtttcacca aaacgacgga ccgccgcgca
     2221 aggatcgagg cggcggccaa ggccatccat gaccatgaac agcagttgag acatgccatg
     2281 gcgcacggc
//
LOCUS       DAORVN010000349         2270 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_48672_length_2270_cov_2.805869, whole genome shotgun sequence.
ACCESSION   DAORVN010000349 DAORVN010000000
VERSION     DAORVN010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2270
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48672_length_2270_cov_2.805869"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="PKV23_07465"
     CDS             complement(<1..242)
                     /locus_tag="PKV23_07465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HQX85309.1"
                     /translation="MTSRFSFAPLVFVVFLMVPIYWLFIMSVKSIGEISGAVTFYPHA
                     PTVENFRFILNDPSWFYGFVNATIYVLMNVVISIVVA"
     gene            complement(239..1051)
                     /locus_tag="PKV23_07470"
     CDS             complement(239..1051)
                     /locus_tag="PKV23_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012654579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HQX85310.1"
                     /translation="MALSGVVPMAFVAFYSLHDTFAGNSFIWVGLDWYERVLTSPEFF
                     RALGRSLFFSVLVLLVEIPLGIFIALRMAPSGLMTSTYIVLMAIPLLTPSIVVGYLWK
                     AMSLEQAGLLYEIAGWFGLAYNMNSVGVSWLTLVLMDVWHWTSLVVLLCFAGLRAIPD
                     DYYRAARIDGANAWAIFRYIQLPKLRLVLLIAILLRFMDSFMIYAETYVVTRGGPGVS
                     TTLLSHELVQTATVQFDLGEAGAMSVIYFLIILCVSWAFFTLIVPRERGREA"
     gene            1415..>2270
                     /gene="dnaN"
                     /locus_tag="PKV23_07475"
     CDS             1415..>2270
                     /gene="dnaN"
                     /locus_tag="PKV23_07475"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007872890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HQX85311.1"
                     /translation="MRVTIERSSFLRALNHVQSVVERRNTIPILSNVLIQAAGANLRL
                     TATDLDLEIVETVTAEVGKEGSATVPAHMLYDIVRKLPEGAQLELDQGPDAGRISVFA
                     GKSRFSLQALPPEDFPDLASGDLANTFSIAAADLKGLIEKTRFAISTEETRYYLNGIY
                     LHEVAQGSLLRAVATDGHRLAQAQIALPKGAKGMPGIIVPRKTVLDVVKLLEGEDGEV
                     EIALSSSKIRFTVGNLVLTSKLIDGTFPDYERVIPRSNDKVLDVDTRTFADAVDRVST
                     ISMEKGRAV"
BASE COUNT          539 a          698 c          637 g          396 t
ORIGIN      
        1 gcaaccacaa tcgaaatcac cacgttcatc aacacataga tcgtggcgtt gacaaagccg
       61 taaaaccagc tcgggtcgtt caggatgaag cggaaattct ccaccgtcgg cgcatgcgga
      121 tagaaggtaa cagcgccgct gatctctcca atgctcttca cgctcatgat gaacagccag
      181 tagatcggca ccatgagaaa caccacgaaa acaagcggcg caaaggaaaa gcgcgaggtc
      241 atgcttccct gccccgttcg cgcggcacga tcagcgtgaa gaaggcccac gagacgcaca
      301 gaatgatcag gaaatagatg accgacatgg cccccgcctc gccgaggtcg aactgcaccg
      361 tcgccgtctg caccagctcg tgtgacagaa gcgtggtcga aaccccgggg ccgccccggg
      421 tgaccacata ggtttccgca tagatcatga agctgtccat gaagcgcagc aggatggcga
      481 tgagcagcac cagccgcagc ttcggaagct ggatatagcg gaagatggcc caggcattcg
      541 ctccgtcgat gcgcgccgcg cggtaataat cgtcagggat ggcgcgaagg cccgcaaagc
      601 ataaaagcac caccaggctg gtccagtgcc agacgtccat cagcaccagg gtgagccagg
      661 aaacgccaac cgagttcatg ttgtaggcaa gcccgaacca gccggcaatt tcatacaaaa
      721 gccccgcctg ctccagtgac atggctttcc acagatagcc caccacgatg gaaggcgtga
      781 gcagcggaat ggccatcagc acgatatagg tggaggtcat cagccccgac ggcgccatgc
      841 gcagcgcgat gaagattccg agcggaattt ccaccagcag gacgaggacg gaaaagaaaa
      901 ggctgcgccc cagcgccctg aaaaactcgg gcgaggtcag aacccgctcg taccagtcca
      961 accctaccca gataaaactg ttgccggcga acgtgtcatg cagcgaatag aacgcgacaa
     1021 aggccatggg cacgacgccg ctcagcgcca tcacgaggag tgccggggcc aggaaggcat
     1081 aacccgcatt gctctgcagc ttgacgctca cggaagtgca aaacccctgg ataatatcct
     1141 gtcatcgaag ggatgatcag cctactaatg ctgagattga tatgaaaagt cgccattggc
     1201 aagtggactg gtcacaccag gttcgcaggg tccgctttgg gtcaaaagcg gaaattcatg
     1261 ctgatccact gcctttccac aggaagcctt gaacctcggc ctctttcggg ccattgttta
     1321 cggcccacaa ggggtccaga gcaagtcccg ccaaagtgtg aagcggtttg gcgataaggg
     1381 catgctccaa gtcaatgaca ggggtatttc cgttatgcgt gtgacgattg aacgatcttc
     1441 gtttctcagg gcgctcaatc atgtgcagag tgtggtggag cggcgcaaca ccattccgat
     1501 cctctccaat gtgttgatcc aggcggccgg cgccaacctg cgcctgaccg ccaccgacct
     1561 tgatctcgag atcgtcgaaa ccgtcactgc cgaagtgggc aaggaaggct cggccacggt
     1621 tcccgcccac atgctctatg atattgtccg caagctcccg gaaggggccc agcttgaact
     1681 tgaccagggc cccgatgcgg gccgcatttc ggtgttcgcc ggcaagtcgc gcttttccct
     1741 gcaggccctg ccgcccgagg atttccccga tctcgccagt ggcgatctcg ccaatacttt
     1801 ttcgatcgcc gctgccgatc tcaagggcct catcgaaaaa acccgttttg cgatttcaac
     1861 cgaagagacc cgctattacc tgaacggcat ctatctccat gaggtggctc aagggtcgct
     1921 gctccgcgcc gtggcaaccg atggccaccg gctggcccag gcccagatcg cgctccccaa
     1981 gggcgccaag ggcatgcccg gcatcatcgt cccgcgcaag accgtgctcg acgtcgtgaa
     2041 gcttctcgaa ggcgaggatg gcgaggttga aatcgccctg tcatcctcca agatccgttt
     2101 caccgtcggc aatctggtgc tcacctcgaa actcatcgac ggcaccttcc cggattacga
     2161 gcgcgtcatc ccgcgcagca acgacaaggt gctggacgtc gacaccagaa ccttcgccga
     2221 tgcggtggac cgcgtctcca ccatatcaat ggaaaagggc cgcgccgtga
//
LOCUS       DAORVN010000350         2263 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_48956_length_2263_cov_3.277174, whole genome shotgun sequence.
ACCESSION   DAORVN010000350 DAORVN010000000
VERSION     DAORVN010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2263
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48956_length_2263_cov_3.277174"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..768
                     /locus_tag="PKV23_07480"
     CDS             <1..768
                     /locus_tag="PKV23_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007535217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HQX85312.1"
                     /translation="NYSVQETFGNNEFFWSGTIWFQQLLENDQIRDSLGRQIAFTAVA
                     LFIQVPLGLAIALCMPRKGPWVSVCLVLMALPLLVPWNVVGSMWNIMALPDIGLLGKT
                     INAMGIHYNYTRDPVAAWFTVVLMDVWHWTSLVVLLCYAGLVSIPDAYYQAAKIDGAR
                     PWAVFRYIQLPKLKHVLTIAILLRFMDSFMIYTEVIVLTGGGPGSATKFLSIDLVQKA
                     LGGFDLGPAAAFSIVYFLIILLTSWLFYTLMMRNEAN"
     gene            768..1565
                     /locus_tag="PKV23_07485"
     CDS             768..1565
                     /locus_tag="PKV23_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006931839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HQX85313.1"
                     /translation="MSKRYLVPIVYIIFLMLPIYWLLNMSFKTTNEILNSFSLYPREF
                     TLDSYRTIFTDPSWYGAYINSITYVALNTVLSVALALPAAYAFSRYRFLGDKHLFFWL
                     LSNRMAPPAVFVLPFIQLYSSVGLFDTHLAVAFAHMLFNIPLAVWILEGFMSGVPKEL
                     DETAYVDGYSFPKFFVKIFIPTISAGIGVAAFFCFMFSWVEMLLAKNLTSVAAKPIVA
                     AMTKTSSASGYELGLLAAAGVLTLIPGAIVVYFVRNYIAKGFALGRV"
     gene            1578..1859
                     /locus_tag="PKV23_07490"
     CDS             1578..1859
                     /locus_tag="PKV23_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018235193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2160 domain-containing protein"
                     /protein_id="HQX85314.1"
                     /translation="MGISWMAWTWPTALFFIYIFGSIAVMGVWEYYSPGGNPRRGIFA
                     LDTTRGDRLFITHLGTCFIFLAWLGLFGVPLWGGLVLALIWGFVVFKWV"
     gene            complement(1960..>2263)
                     /locus_tag="PKV23_07495"
     CDS             complement(1960..>2263)
                     /locus_tag="PKV23_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008553140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HQX85315.1"
                     /translation="KMRAALNAFDLLPVSRDFAFVIDDSVEADKLIKAAKGADKALIS
                     DAFVFDVFKLEGAKKSYAIEVTLQPRDKTLTDEDIEAVSQKIIAAVQKATGGTLRS"
BASE COUNT          389 a          725 c          579 g          570 t
ORIGIN      
        1 aactattcgg tccaggaaac cttcggcaac aatgagttct tctggtcggg caccatctgg
       61 ttccagcaat tgctggagaa cgatcagatc agggattcgc tcggacgcca gattgccttc
      121 accgcggtgg cattgttcat tcaggttccg ctgggcctcg caatcgccct ctgcatgccg
      181 cgcaaaggcc cctgggtgtc ggtctgcctt gtgttgatgg ctctgcctct gcttgtgccg
      241 tggaacgtcg tgggctccat gtggaacatc atggccctac cggacattgg ccttctcggc
      301 aaaaccatca atgccatggg catacattac aattacacgc gcgatccggt tgccgcctgg
      361 ttcaccgttg tcctcatgga tgtctggcac tggacctcgc ttgtcgtgct gctgtgctat
      421 gccggcctcg tctccatccc cgatgcctat taccaggccg ccaagatcga cggcgccagg
      481 ccctgggcgg tgttccgtta catccagctg ccgaagctca agcatgtgct gaccatcgcc
      541 atcctgctcc gcttcatgga cagtttcatg atctacacgg aagtgatcgt gttgactggc
      601 ggcgggccgg gcagcgccac caaattcctc tccatcgacc tggtgcaaaa ggcccttggc
      661 ggctttgacc tcgggccggc ggcagcgttc tccatcgttt acttcctgat cattctgctc
      721 accagttggc tgttctacac cctcatgatg cgaaacgagg caaactgatg tcgaagcgct
      781 atctcgttcc cattgtctat atcatcttcc tgatgctgcc gatctactgg ctgctcaaca
      841 tgtcgttcaa gaccaccaac gagatcctga acagcttttc gctctacccc cgcgagttca
      901 cccttgacag ctaccgcacg atcttcactg atcccagctg gtatggcgcc tacatcaatt
      961 ccattaccta tgtggcgctg aacacggtcc tctccgtggc tctggcgctg cctgcggctt
     1021 acgccttctc gcgctaccgt tttctcggcg acaagcacct gttcttctgg cttttgtcga
     1081 accgcatggc gccgcccgcc gtgttcgtct tgcccttcat ccagctctat tcatcggtcg
     1141 gcctctttga cacccatctc gccgttgctt tcgcccacat gctgttcaac attcccctgg
     1201 ccgtgtggat tctcgaaggc ttcatgtccg gcgttcccaa ggagctggac gagaccgctt
     1261 atgtcgacgg ttactccttc ccgaaatttt tcgtgaagat tttcattccg acgatttccg
     1321 caggcattgg cgtggccgcg tttttctgtt tcatgttctc ctgggtggaa atgctgctgg
     1381 ccaagaacct gacctccgtg gcggccaagc ccatcgtcgc ggccatgaca aaaacctcca
     1441 gcgcctcggg ctatgaactg ggcttgctgg cggcggccgg tgtcctcacc ctcatcccgg
     1501 gcgccatcgt cgtctatttc gtccgcaact atatcgccaa gggctttgcc ctgggccgcg
     1561 tgtaaggagg ctttgaaatg ggcatttcat ggatggcctg gacctggccc accgccttgt
     1621 tcttcattta tatcttcggt tccatagcag tcatgggcgt ctgggaatac tattcccccg
     1681 gcggcaatcc gcgccgcggc atttttgccc tcgataccac gcgcggcgac cggctcttca
     1741 tcacccatct cggcacctgc ttcatcttcc tcgcctggct cggactcttt ggcgtccccc
     1801 tctggggcgg cctcgtgctg gcgctgatct ggggctttgt ggttttcaag tgggtgtaaa
     1861 aaacttctgt catcccggcg aaagccggga cccagacttt gaaccactca tctgctgaaa
     1921 gccagggccc cgactttcgt cggggtgaca gacaaaaaat taactcctta acgtcccgcc
     1981 cgttgccttc tgcacagccg cgatgatttt ctgcgacacc gcctcaatat cttcatcggt
     2041 caaggtcttg tcgcgcggct gcagcgtaac ctcgatggca taggacttct tcgcgccttc
     2101 cagcttgaac acgtcaaaca caaaagcgtc ggaaatcagc gccttgtccg cacctttggc
     2161 cgccttgatc agcttgtcgg cctcgacgct gtcatcgatg acaaaggcga agtcgcgcga
     2221 cacgggcagc agatcgaagg cattgagcgc ggcgcgcatc ttc
//
LOCUS       DAORVN010000351         2260 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_49080_length_2260_cov_2.137868, whole genome shotgun sequence.
ACCESSION   DAORVN010000351 DAORVN010000000
VERSION     DAORVN010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2260
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49080_length_2260_cov_2.137868"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..422)
                     /locus_tag="PKV23_07500"
     CDS             complement(<1..422)
                     /locus_tag="PKV23_07500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine N-6-methyltransferase family
                     protein"
                     /protein_id="HQX85316.1"
                     /translation="MTSIDRYNYAFDPEGDAWAARLLRRLPPQGDVLELGPGPGAMTR
                     VMVDRGYRVTVVENDPGALKCLEALGVEVIAADKAGVLHQLAEFFVRHGITVEQLQST
                     RYKAMQTGADMFSAQITIGIPASTHIAGLRDDFLEFCDG"
     gene            complement(419..1312)
                     /locus_tag="PKV23_07505"
     CDS             complement(419..1312)
                     /locus_tag="PKV23_07505"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85317.1"
                     /translation="MKYDRSLDLVIPLMARIPTTLPWRVAGRAGGGQTADQHSRLLRW
                     LEDCFARVFPHATGAQHVQWAQAHVLMLAQEKVDAMAFKRLGRSGGPSIAMQGSEHAK
                     ALAREGKGFILVLNHFDRLLTAPVALAQQGIVTNVLTMPVLENPDLGEAQRRFLLKKI
                     NGYTQVTGGRWHTTSEGLRPVHESLRAGQAWVILADAWRPEFGRLRNHGFLDGSLQLP
                     TGIERLAQSTGVPLLHAITYTQRPSHLQVIVEPLPENPREAIDKVIRRLDQDVQDRPW
                     AWWHWGLWEQMWHPTTKEGEL"
     gene            complement(1309..>2260)
                     /locus_tag="PKV23_07510"
     CDS             complement(1309..>2260)
                     /locus_tag="PKV23_07510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85318.1"
                     /translation="GAGFHPEESGHDNIRFMAALHGLEGMAMEDFVVRAIAFSELTAE
                     TLERPVKTYSSGMFMRLAFAAATAIEPDVLIVDEALSVGDMHFQKKSLNRILGMRERG
                     ATILFCSHNLYQVRSLCQRAAWIHGGRIQAIGATEDVVTAYEAHERRRYAHLRAADTD
                     AGAQQAASDRPVTSSADVPLAKIVRLMIETADGINPAQVDSFQDLAVEIELESYGNAP
                     FHVLFAIVRPDKDNVFGTSTQFRAQGEPLKGTGTHRIRMRFPRLSLLSGEYLWSVYVL
                     DDTGIQVLDMAELIQPFTVLNQKRQEFGVAWLEHEWVLVE"
BASE COUNT          497 a          717 c          593 g          453 t
ORIGIN      
        1 ccgtcgcaaa attcgagaaa atcgtcacgc aagccggcga tatgcgtgga agcgggtatg
       61 ccgatggtta tctgtgcgga aaacatgtcg gcgccggtct gcatcgcctt gtaacgcgtg
      121 gattgcaatt gctcgacagt gattccgtgg cgcacgaaaa attcggcgag ttgatgaagc
      181 acgccggcct tgtccgctgc aatgacctcc acccccaggg cttccaggca tttaagtgcg
      241 ccgggatcgt tctctacgac ggtcacccga taaccccggt ccaccatgac ccgagtcatg
      301 gcacctggcc cagggccgag ctcgagcaca tcgccttgtg gcggaagtcg gcgcaacaag
      361 cgggcagccc aggcgtcacc ctccgggtca aatgcgtagt tgtatcgatc aatactggtc
      421 aaagctcgcc ttcctttgtg gtcggatgcc acatctgctc ccacaaaccc caatgccacc
      481 aagcccaggg acggtcttgc acatcttgat caagacgtcg aatgaccttg tcaatggcct
      541 ctctggggtt ttctgggagg ggctccacaa tcacttgcag gtgactcggt cgctgcgtgt
      601 aagtgatggc atgcaacaaa ggcactcctg ttgactgtgc caagcgttca atacccgtgg
      661 gcaactgcag actaccatca agaaacccgt ggttccgcag ccgtccgaac tcgggacgcc
      721 aagcgtctgc cagtatcacc caagcctgcc cagcccgcaa actttcatgt acaggacgca
      781 atccttcact ggtagtgtgc cagcgccctc ctgtaacctg ggtatagcca ttgattttct
      841 tgagtaaaaa ccggcgttgc gcctccccaa gatcgggatt ttccagcacc ggcatcgtca
      901 ggacattggt aacaatccct tgttgagcca gtgcaaccgg ggccgtcaac agccgatcaa
      961 agtgattgag caccaagata aaccccttgc cttcccgcgc cagggctttg gcatgctcac
     1021 tcccctgcat tgcaatggag ggcccgccac tccgacctag acgcttgaaa gccatggcgt
     1081 ccaccttttc ctgcgcaagc atgagcacat gggcttgcgc ccattgcaca tgctgcgcac
     1141 ccgtggcgtg cggaaacacc cgtgcgaagc aatcctccag ccagcgcagc aatcggctgt
     1201 gctgatcagc ggtctgccca cctcctgcgc gacctgcgac ccgccaaggt aacgtcgtgg
     1261 ggatgcgagc catcaaagga atgaccaagt ccagcgagcg gtcgtacttc attcaacaag
     1321 cacccattca tgctcaagcc acgccacacc aaactcttgg cgcttctggt tcaatacggt
     1381 gaatggctgg atcagctcag ccatgtcgag cacctggatc cccgtgtcgt ccaggacgta
     1441 gacactccac aagtactctc ccgatagcag cgacaaacgc ggaaaacgca tgcgaatccg
     1501 gtgtgtcccg gtgcccttca gcggctcgcc ttgtgcacgg aactgggtgc tggtaccaaa
     1561 cacattgtcc ttgtctggtc ggacaatcgc aaacagcaca tgaaacggag cattgccata
     1621 agactcgagc tcgatttcca cagccaagtc ctgaaaagag tccacctgcg ccggattgat
     1681 gccatcggca gtctctatca tcaggcgcac aatttttgcc agtggcacat ctgcagacga
     1741 cgtcacgggc ctgtcagatg ccgcctgctg cgcccccgca tcggtgtcgg cggcacgcag
     1801 atgggcatac cgcctgcgct catgggcctc ataggctgtc accacatcct cggtggcacc
     1861 gatggcctgt atgcgccccc catggatcca ggccgcgcgc tggcaaagac tgcgcacctg
     1921 gtacaggttg tgggaacaaa aaagaatggt cgctccacgc tcgcgcattc ccaaaatgcg
     1981 gttcaaactc tttttttgaa aatgcatgtc ccccacggac agcgcttcat ccacgatcag
     2041 cacatccggc tctatggccg tagctgccgc aaacgccaag cgcatgaaca tgccagagga
     2101 atacgtcttg accgggcgtt ccagtgtctc cgctgtcagt tctgaaaatg cgatagcccg
     2161 aacgacaaag tcttccatgg ccatgccttc cagcccgtgc aaggctgcca tgaaccggat
     2221 gttgtcatgg cccgattctt cgggatgaaa ccccgcgccc
//
LOCUS       DAORVN010000352         2254 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_49296_length_2254_cov_2.833561, whole genome shotgun sequence.
ACCESSION   DAORVN010000352 DAORVN010000000
VERSION     DAORVN010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2254
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49296_length_2254_cov_2.833561"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..325
                     /locus_tag="PKV23_07515"
     CDS             <1..325
                     /locus_tag="PKV23_07515"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: protein motif:HMM:NF014685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HQX85319.1"
                     /translation="FSRSLVVDLLWATRPARSDGLSVFAGRPGKRSALFSLLTGGIGA
                     AVAVGLALSPEIAVIAIIAAGIVLAGLRALAMGKIGGQTGDVCGAVQVLTELTMLTVY
                     AATIG"
     gene            413..892
                     /locus_tag="PKV23_07520"
     CDS             413..892
                     /locus_tag="PKV23_07520"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02281 family clan AA aspartic protease"
                     /protein_id="HQX85320.1"
                     /translation="MRLPGALSPIGLALLFTIAGGLPGITPLAAADMLQLNAATALKA
                     GQNGHFIVKAEINGRNVRVMVDTGASAVALSYEDAQDVGLRPGSLDYNVPVSTANGVV
                     KAAGVNLDKVEIDGVRVTDVQGLVMPKGVMRGSLLGMSYLAKLKSFKVEDGVLYLKN"
     gene            960..1196
                     /locus_tag="PKV23_07525"
     CDS             960..1196
                     /locus_tag="PKV23_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013531716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HQX85321.1"
                     /translation="MNAFDLPEVETPCIKVCVVDPETGFCIGCGRTRMEIGGWLGFSP
                     AERQSIMAGLNERVETLTLRKRRRGGRQGRLANS"
     gene            complement(1193..1597)
                     /locus_tag="PKV23_07530"
     CDS             complement(1193..1597)
                     /locus_tag="PKV23_07530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85322.1"
                     /translation="MTGYWYSGTWRYSPSLTVAWSKEWQDASGALNAQTIETGIISPG
                     FQVGNSISIGGASTVEPWLGAQLDWVGINKAVDDVTGTILDDPYTDLRLQGGLNFAFG
                     ANTQLTLTAEISGLLNEESDTYTAGANFAFQF"
     gene            complement(1622..>2254)
                     /locus_tag="PKV23_07535"
     CDS             complement(1622..>2254)
                     /locus_tag="PKV23_07535"
                     /inference="COORDINATES: protein motif:HMM:NF015737.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HQX85323.1"
                     /translation="AFVLSAGIAGSSQALAQSAYGDECHELDDTSPEIVTACAKSLLN
                     RQSLINTTASITGVILNRFDRGPNQAEPAGLELANREEIADRLSGGKSILIAPTADVA
                     APPASPMWNIWIDGKYSWLDDTDAFTDLDGSLVNVVAGFDYKVSDRLVLGLMGTYESS
                     DLDGSGVTQDTDGWGGGAYLGMTLTDNVVLSATLLGTVLVTEVNGGTSFD"
BASE COUNT          467 a          625 c          680 g          482 t
ORIGIN      
        1 attttcccgt tccctggtgg ttgacctgtt gtgggcgacg cggccggcgc gcagtgacgg
       61 gctatcagtc ttcgcgggaa ggcccggaaa acgcagcgcc ctgttctccc ttcttacggg
      121 tggcatcggt gctgcggtgg cggtcgggct tgccctgtcg cctgaaatcg cggtgatcgc
      181 catcatcgcc gccggcattg ttcttgcggg tttgcgggcg ctggccatgg gcaagatcgg
      241 cgggcagacc ggcgatgtct gcggcgccgt ccaggtgctg acggaactca ccatgctcac
      301 agtgtatgcc gcaacaatcg gttaacgtta acggcatttg ttccaattgt tttcaacact
      361 tgttgagagt ggcccgggca agctgaggca acttaatcca gggagttgcg tcatgcgttt
      421 gccgggtgcg ttgtcaccga ttggacttgc cttgctgttt acgattgccg gcggcctgcc
      481 gggcataacg ccgctcgcgg cggcggacat gctccaactc aacgcggcca cggcattgaa
      541 agccggacaa aatggccatt tcatcgtcaa ggccgagatc aatggccgga atgtcagagt
      601 gatggtcgat acgggggcct cggcggtggc cttgagctac gaagatgccc aggatgttgg
      661 cctccgcccg ggcagccttg actacaatgt tccagtttca accgccaatg gcgtggtcaa
      721 agccgccggt gtcaacctcg acaaggttga aattgacggg gtgcgcgtca cagatgtgca
      781 agggctggtg atgcccaagg gcgtcatgcg cggttcgctg cttggcatga gctatctggc
      841 caaattgaag agtttcaaag ttgaagacgg cgtgctttat ctgaagaact gagacgcttt
      901 tgcttcaata atggcgcgtt cggatggcca atcacccaca ggatacctat cttagcggca
      961 tgaacgcctt tgatttgcca gaagtcgaga caccctgcat caaggtttgc gtggtcgatc
     1021 cggaaaccgg attctgcatc ggctgcgggc gcacgcgcat ggaaatcggc ggctggcttg
     1081 gttttagccc ggcggaacgc cagtccatca tggcagggct taacgagcgg gttgaaacgc
     1141 tcacgctgcg caagcgccgc cgcggcgggc gccagggacg cctcgccaat tcctagaact
     1201 ggaatgcgaa atttgcgccg gccgtataag tatctgattc ctcgttgaga agcccgctga
     1261 tttccgcagt caacgtcagt tgggtattgg ctccaaaagc aaaattgagg ccgccttgca
     1321 atcgaagatc cgtataggga tcatccaata ttgtgccggt cacgtcatca actgccttgt
     1381 ttatcccgac ccaatccaat tgcgcgccga gccagggctc aaccgttgaa gcaccgccga
     1441 tggatatgga gttgccgacc tggaagcccg gcgaaatgat cccggtttcg atagtctggg
     1501 cgttcaatgc accgcttgcg tcctgccatt ccttggacca tgcgacggtc agcgagggcg
     1561 aataacgcca ggtgccggaa taccaataac cggtcaaggc gctgctggcc tgaatccggg
     1621 tctagtcaaa actggtgccg ccattcactt ccgtgacaag tacggttcca aggagagtgg
     1681 ccgacaacac cacattgtcg gtgagtgtca tacccagata tgccccgccg ccccagccat
     1741 cggtatcctg ggttacgcct gatccatcca agtctgagga ttcataggtg cccatcaggc
     1801 ccagcaccag acggtccgag actttgtaat cgaaaccggc aacaacattt acaagcgagc
     1861 cgtcaagatc ggtaaaagcg tccgtgtcat ccagccagct gtattttcca tcgatccaga
     1921 tgttccacat gggactggca gggggagccg caacatctgc agttggcgca atgaggatgg
     1981 atttgccgcc ggaaagacga tcagcaattt cttcccggtt cgccagttca aggccggcag
     2041 gctcagcctg gttcggcccc ctgtcaaacc ggttaagaat gacgccagtg atagaggccg
     2101 tggtgttgat aagggactgg cggttcaaca aggacttggc gcaggctgtg acgatttcgg
     2161 gcgaggtatc gtcgagttcg tgacattcat cgccgtaagc ggattgggcc agcgcctggg
     2221 aactgcctgc gatgcccgcc gataagacaa aggc
//
LOCUS       DAORVN010000353         2253 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_49345_length_2253_cov_2.471793, whole genome shotgun sequence.
ACCESSION   DAORVN010000353 DAORVN010000000
VERSION     DAORVN010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2253
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49345_length_2253_cov_2.471793"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            3..1142
                     /locus_tag="PKV23_07540"
     CDS             3..1142
                     /locus_tag="PKV23_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018387583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HQX85324.1"
                     /translation="MPRLPADIYSHIHDTARRFASDRVRLRAAELDETEAFPAELYEE
                     MAQLGLFGITVPEDFGGAGLDALSYSLVMEELSRGYASVADQCGLVELIGTLLSVHGT
                     KTQREMYLSDVLAARKRVSYCITEAEAGTDVSGVKTTARRDGPDWRLSGSKIWIHNAP
                     IADYGYVLARTDPDAGNRGMSIFIVDLSAEGVSRGKKEKKMGQRASPVGALHFEDVRL
                     SADALLGPINRGFHIMMSVLDKGRIGIGALAVGIAQAGLDAALDYAKTRKQFGKPIAE
                     NQGLQWVMADMKKDITAARALVHRAAMTMDAGLPATMDCSIAKCFASDTAVQHTANAV
                     QIFGGSGYIRGFEVERLYRDAKITQIYEGANQIQRSIIARELLKA"
     gene            1148..1585
                     /locus_tag="PKV23_07545"
     CDS             1148..1585
                     /locus_tag="PKV23_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018387568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HQX85325.1"
                     /translation="MAEFQTDIIGLTATFRKTVSESDVYLFAGITGDFSPNHVDEEFM
                     RGTQYGGRIAHGALLVGYMSRASTMIVEKCVSVTSSTYPVSIGYDRTRFLKPVYIGDT
                     ITVTYVMAAYDEARSRSSATIEITKGSSEVVASAIHLMKWLNR"
     gene            complement(1589..>2253)
                     /locus_tag="PKV23_07550"
     CDS             complement(1589..>2253)
                     /locus_tag="PKV23_07550"
                     /inference="COORDINATES: protein motif:HMM:TIGR01459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR01459 family HAD-type hydrolase"
                     /protein_id="HQX85326.1"
                     /translation="NISRLAKLGFDSSSFDHFVTSGDVAPALLESGLLPIERSKSTRC
                     LTIASGEDRSLADNLGFSSVDTAEEADLVIISGSETERIPLASYRALLLPAAKRGVPC
                     ICTNPDVHKLVGETLLPAAGTIAALYEEMGGKVTRIGKPYVDIYRHAHALCGHPDKAR
                     IVAIGDSLEHDVIGAATFGIDSALTLTGLQAHVSEAALLAQMQEGQIAPRYLLGSFVW
                     IE"
BASE COUNT          480 a          648 c          675 g          450 t
ORIGIN      
        1 ccgtgcccag gctgccggcg gatatttaca gccacatcca tgatacggcc cggcgctttg
       61 ccagcgaccg ggtacgcctc cgcgccgccg agcttgatga aacggaggcc tttcccgccg
      121 agctctacga ggaaatggcg caactcggcc ttttcgggat cacggttccg gaggacttcg
      181 gcggtgcagg gcttgatgcc ctctcctatt cccttgtcat ggaagagctt tcgcgcggct
      241 atgcctcggt ggccgaccaa tgcggcctcg tggagctgat cggcacgctt ctctcggtgc
      301 atggaaccaa gacccagcgc gagatgtatc tttccgacgt cctcgccgcg cgcaagcggg
      361 tgagctattg catcaccgag gctgaggccg gcacggatgt ttcgggcgta aaaaccaccg
      421 cccggcggga cggcccggac tggcgtcttt ccggctccaa aatatggatc cacaacgctc
      481 ccatcgccga ttatggctat gtccttgccc gcaccgatcc cgatgcaggc aaccgtggaa
      541 tgtcgatctt catcgtcgat ctctccgccg aaggggtatc gcgcggcaag aaggaaaaga
      601 aaatgggcca gcgcgcatcg ccggttggcg cgctgcattt cgaagatgtg cgattgagcg
      661 ccgatgcact actgggccca atcaaccgcg gctttcatat tatgatgagc gttctcgaca
      721 agggccgcat cggcatcggg gcactggctg tcggcattgc ccaggcgggg cttgacgcgg
      781 ccctcgacta tgccaagacc cgcaagcaat tcggcaagcc catcgccgaa aaccagggcc
      841 tgcaatgggt gatggctgac atgaagaagg acatcacggc ggcacgcgcg ctggtgcacc
      901 gcgcggcgat gacgatggat gcgggcttgc ctgcgaccat ggattgctcg atcgccaaat
      961 gctttgcaag cgacacggcg gtacagcaca cggcaaacgc ggttcagatc tttggcggtt
     1021 cgggatatat ccgcgggttt gaggtcgaaa ggctctatcg cgatgccaaa atcacccaga
     1081 tctacgaggg cgccaaccag attcaacgca gcatcatcgc ccgcgaactg ctcaaggcct
     1141 aacgcaaatg gctgaattcc agactgacat catcggattg acagcgacct tccggaaaac
     1201 cgtttctgaa agcgacgtct atctctttgc cggcatcacc ggcgatttct cgcccaacca
     1261 tgtggatgaa gaattcatgc gcgggaccca gtatgggggg cgcattgccc atggcgcgct
     1321 gctggtaggc tacatgtcgc gggcctcgac gatgattgtg gagaaatgcg tcagtgtaac
     1381 ttcttcgacc tatcccgtat caatcggcta tgacaggacc aggttcctga agccggttta
     1441 catcggcgac acgatcaccg tgacctatgt catggcagcc tatgatgaag cgcggagccg
     1501 ctcatcggcc actattgaaa tcaccaaggg tagtagcgaa gtcgtggctt cggcgatcca
     1561 tctgatgaaa tggctcaaca ggtagccttt attcaatcca cacgaaagac ccaagcagat
     1621 aacgcggggc aatttgccct tcctgcattt gggcgagaag tgccgcctcc gaaacatgag
     1681 cctgcaaacc agtcaaagtc aatgcgctgt ctatgccaaa ggttgcggca ccaattacat
     1741 cgtgttcaag gctgtcgccg atcgcaacga tccgcgcctt gtcgggatgg ccgcacaagg
     1801 cgtgggcatg gcggtagatg tccacatagg gcttaccgat gcgggtgacc ttgccgccca
     1861 tctcttcata tagcgcagca atggttccgg cggcaggaag aagcgtttcg cctacaagct
     1921 tgtgaacatc agggttggta cagatgcatg gcacgcccct ttttgccgcg gggagcagga
     1981 gagcgcgata ggacgcaagc gggatacgct cggtttcgct gccggagatg atgaccagat
     2041 cggcttcttc ggcagtatcc acgctggaga aacccagatt gtcggcgaga gaacggtcct
     2101 cgccacttgc tattgtcagg cagcgggtgc ttttgcttct ctcaatgggg agcagaccag
     2161 attccagcaa ggcgggtgca acgtcgccgg aggtgacgaa gtgatcgaat gagctgctgt
     2221 cgaagccgag tttcgccagg cgggagatgt tgt
//
LOCUS       DAORVN010000354         2252 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_49360_length_2252_cov_3.547110, whole genome shotgun sequence.
ACCESSION   DAORVN010000354 DAORVN010000000
VERSION     DAORVN010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2252
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49360_length_2252_cov_3.547110"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..887
                     /locus_tag="PKV23_07555"
     CDS             <1..887
                     /locus_tag="PKV23_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014495981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQX85327.1"
                     /translation="SRVTRYDAVIPPAPPASDYPVETFVGDLSAAGEAEKLIAGKPDV
                     IFHLAAIVSGEAEKDFEKGYRINLDATRILFEVIRKAGGGYKPKVIFTSSIAVFGAPF
                     PEAIGDEFFTTPLTSYGTQKAIDELLLNDYSRRGFFDGVALRMPTVCVRPGKPNLAAS
                     GFFSNIIREPLAGMEAVLPVSDTVRHWHASPRAAVLFLIQAATMDTQKLGNRRTLTLP
                     GLSCTVAEQIEALRRVAGNAVVKLIRREPDETIAKIVSGWPRNFDPRRALDLGFKAEG
                     SFEDIIRVHMEDELAPCK"
     gene            951..1844
                     /locus_tag="PKV23_07560"
     CDS             951..1844
                     /locus_tag="PKV23_07560"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HQX85328.1"
                     /translation="MSPTDSHENRMRGILWMLATMLCFITLDAIMKHLLESYSLVQVT
                     WARFFFATIIAALVCGRNLGRLARTISPGKQAIRSLLLMSTTAVFNAGIRTTPLATAT
                     TIMFLSPLLVTLLSIPLLGEKVGLRRWAGIVVGFTGAVIVVQPWQSGFGGIGLGVLFL
                     LAAALLNANYQIVTRMVRDDDPLTSLLFTATAGAVVTSLIVPWFWSWPTAFDWLLLIG
                     SGLAGAIGHLCLIRALRLAPASVVAPFSYSSLVWATLFGFVIWGDWPNLWTWLGAALI
                     IGSGLYIFHRERRHLSVAVEA"
     gene            1847..>2252
                     /locus_tag="PKV23_07565"
     CDS             1847..>2252
                     /locus_tag="PKV23_07565"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HQX85329.1"
                     /translation="MRSNRNLLGIASLIFGVMLFSTQDAIIKAISNDHAVTLAMAIRC
                     MVSLPLLLVLVHFECGIAKLRSPLFWALVMRGGILLVAYTTYYMGLPALPLAEAIALF
                     FAAPIIVTILSVPMLGEKVSGMSWAAIALGFVG"
BASE COUNT          384 a          765 c          590 g          513 t
ORIGIN      
        1 tatcgcgcgt cacccgctac gatgccgtta ttcctcctgc tccaccagcg tctgactacc
       61 cggttgaaac ctttgtcggc gatctttcgg ctgccggtga agccgaaaaa ctcattgccg
      121 gcaaacctga tgtgatattc caccttgccg caatcgtctc cggcgaagcc gaaaaagact
      181 ttgaaaaagg ctaccgcatc aatcttgatg ccacccgcat cctcttcgaa gtcattcgca
      241 aggctggcgg cggctacaag ccaaaggtca tcttcacctc ttcaatcgcg gttttcggcg
      301 cgccctttcc cgaggccatc ggcgacgaat tcttcaccac gccactcacc agctatggca
      361 cccagaaagc catcgacgaa ctcctgctga atgactattc ccgccgcggt ttctttgacg
      421 gcgtggcgct gcgcatgcca accgtctgcg tccgccccgg caagcccaat ctggccgcat
      481 caggcttctt ttccaacatc atccgtgaac cgcttgctgg aatggaggcc gtgcttcccg
      541 tgagcgacac cgtgcgccac tggcacgcct cgccccgggc cgcggtcttg ttccttattc
      601 aggcagcaac gatggacacg caaaagcttg gaaaccgccg cacccttacc ctgcccggcc
      661 tctcctgcac cgtggccgag cagatcgagg cgctgcgccg ggttgccggc aatgctgtgg
      721 tcaaactcat ccgccgcgaa cccgacgaaa ccattgccaa aatcgtctcc ggctggccgc
      781 gcaattttga tccccggcgg gccctggacc tgggcttcaa ggccgagggc agctttgaag
      841 acatcatccg cgtgcacatg gaagatgagc ttgcgccatg caaatagcgc aaccttgcta
      901 gcatcccagc cacagtagga tagcgcgtcc cagcactcta tagaacgtcc atgtctccaa
      961 cggattccca cgaaaatcgc atgcgcggca tcctctggat gctggctacc atgctgtgtt
     1021 tcataaccct tgacgccatc atgaaacacc tgctcgagag ctattcgctc gtgcaggtca
     1081 cctgggcgcg gtttttcttt gccaccatca ttgccgccct tgtctgtggc cggaatctcg
     1141 gcagacttgc aagaacgatc tcaccgggaa agcaggccat ccgttcgctg cttctcatgt
     1201 ccacaaccgc cgttttcaac gccggcatcc gtaccacgcc gctggccacc gccacgacca
     1261 tcatgtttct cagccccctc ctggtaacac tcctgtccat acccctgctg ggggagaaag
     1321 tgggcctgcg ccgctgggcg gggattgtcg tgggcttcac cggagcggtc atcgttgtgc
     1381 agccttggca aagcggcttc ggcgggattg gcctgggggt gttgttcctg ctcgccgccg
     1441 ccttgctcaa tgccaattat cagattgtca cccgcatggt gcgcgacgat gatcccctca
     1501 cctccctgct tttcaccgcc acagcggggg cggtggtgac aagcctgatc gtcccctggt
     1561 tctggtcctg gcccacggct tttgactggc tgcttttgat tggcagcggg ctggccggtg
     1621 ccatcggcca cctctgcctc attcgcgccc tgcgcctggc gcccgcctcc gtcgttgccc
     1681 ccttcagcta ttcctcgctg gtttgggcca cccttttcgg ctttgtcatc tggggcgact
     1741 ggccaaatct ctggacgtgg ctgggagccg ctctaatcat tggctccggc ctctacatct
     1801 tccaccgcga acgccggcat ctttccgtcg cggttgaagc ctgaccatgc ggtccaaccg
     1861 caatctgctg ggcattgcct cgcttatttt cggcgtcatg ctgttttcaa cccaggacgc
     1921 catcatcaag gccatcagca atgatcatgc ggtgaccctt gccatggcca tccgctgcat
     1981 ggtgtccctg ccgctgctcc ttgtgctggt tcattttgaa tgcggcattg ccaagctccg
     2041 ctcacccctg ttctgggccc tcgtcatgcg cggcggcata ttgttggtag catacaccac
     2101 ctactacatg ggtttgcctg ccctgccgct ggccgaggcc atagccctct tctttgcagc
     2161 gcccatcatc gtgacaatac tctcggtgcc catgctgggt gaaaaggtct ccggaatgtc
     2221 ctgggccgca atcgccctgg gcttcgtggg cg
//
LOCUS       DAORVN010000355         2251 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_49418_length_2251_cov_2.292805, whole genome shotgun sequence.
ACCESSION   DAORVN010000355 DAORVN010000000
VERSION     DAORVN010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2251
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49418_length_2251_cov_2.292805"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(86..358)
                     /locus_tag="PKV23_07570"
     CDS             complement(86..358)
                     /locus_tag="PKV23_07570"
                     /inference="COORDINATES: protein motif:HMM:NF015791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HQX85330.1"
                     /translation="MTELLTPAQMYQADARAIAAGLTEAQLIENAGRAVAEEITRRYG
                     ARKTVVLCGPGNNGADGKVAARYLKQWGWPVTIADDALRLRPQGRR"
     gene            complement(351..1589)
                     /locus_tag="PKV23_07575"
     CDS             complement(351..1589)
                     /locus_tag="PKV23_07575"
                     /EC_number="2.7.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012331660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrophosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HQX85331.1"
                     /translation="MPKPKIAMLTAGGLAPCLSAAVGCLIMDYTKLAPKAEIIGYRSG
                     YAGLLRGISIKVTSRARAKAPLLLKHGGSPLGNSRVKLTNVKDCVKRGLVKEGEDPLK
                     VAAEQLVRDGITILHTIGGDDTNTTAADLAAYLAKHNYRLTVVGLPKTIDNDVVPIKQ
                     TLGALTAAEQGAIFFENVINEQSTSPRMLLIHEVMGRNCGWLTAATAEAYRKRLKKHK
                     FIPEFNLGLDHKELDAVYVPELAIDLEAEATRLTKSMDTFDCVSIFLSEGAGVNDIVA
                     EMRARGEEPERDAFGHVKIDKINPGEWFAKRFAKMLGAEKVLVQKSGYFARSAAANKE
                     DLALIKAMVKHAVKSGLKGESGVVGHDEGRNGLLRTIEFPRIKGGKAFDTQAKWFTRL
                     LKELDQPKGQPVAVSHGGDD"
     gene            2116..>2251
                     /locus_tag="PKV23_07580"
     CDS             2116..>2251
                     /locus_tag="PKV23_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018900556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HQX85332.1"
                     /translation="MKKIEAIIKPFKLDEVKEALQEVGLLGITVTEAKGYGRQKGHTE
                     L"
BASE COUNT          442 a          610 c          701 g          498 t
ORIGIN      
        1 cagcggcagt gcggcgacga accggaatcg atcggggtgg cggacggtaa ggtcggcgag
       61 catgtcgttg atcagcttgg ccgcctcagc ggcgaccttg gggtcggagg cgcagggcgt
      121 catcggcgat ggtgacaggc cagccccatt gcttgagata gcgtgccgcc acttttccat
      181 cggcgccatt gttgccgggg ccgcagagaa caacggtttt ccgtgcgcca tagcgcctgg
      241 tgatttcctc cgccacggcg cgcccggcat tttcgatgag ctgcgcttcg gtgaggccgg
      301 cggcaatcgc ccgtgcatcg gcctggtaca tctgtgccgg ggtcagaagt tcagtcatcg
      361 ccgccatgac ttacggccac gggttgacct tttggctgat ccagttcctt cagcaatctg
      421 gtgaaccact ttgcttgggt atcaaaggcc ttgccgccct tgatacgggg gaactcgatg
      481 gtgcggagga gaccgtttcg tccctcatcg tggccaacga ccccgctttc gcctttcagg
      541 ccgcttttta cggcgtgctt caccatggcc ttgataagag cgaggtcttc cttgttggcg
      601 gcggcggaac gggcgaaata acccgacttc tgcaccagga ctttttcggc gccgagcatt
      661 ttggcaaagc gctttgcgaa ccattcgccg ggattgatct tgtcgatctt cacatggcca
      721 aaggcatcgc gctcgggttc ttcgccgcgg gcccgcatct cggcaacgat gtcgttgacg
      781 ccggcccctt ccgacaggaa gatcgagacg cagtcgaagg tgtccatgga cttggtgagg
      841 cgtgtggctt cggcttcaag atcaatggcg agttccggga catagactgc gtcgagctcc
      901 ttgtgatcga gcccgagatt gaattcggga atgaatttgt gtttcttcag acgcttgcgg
      961 taagcctcgg ccgttgccgc ggtgagccag ccgcagttgc ggcccatgac ttcatggatg
     1021 agcagcatgc gggggctggt gctctgctca ttgatgacgt tctcaaagaa aatggcgccc
     1081 tgctccgccg ccgtcagcgc gccaagagtt tgttttatgg gcaccacgtc attgtcgatg
     1141 gtcttgggga ggcccaccac ggtgagcctg tagttgtgct ttgcaagata ggcggcgaga
     1201 tcggcggccg tggtgttggt gtcgtcaccg ccgatggtgt ggaggatggt gatgccgtcg
     1261 cgcacgagtt gttcggccgc caccttcagc ggatcttcgc cttccttcac cagaccgcgc
     1321 ttcacgcaat ccttgacgtt ggtaagcttc acccggctgt tgccgagcgg cgagccgcca
     1381 tgtttgagga gcagcggagc cttggcgcgg gccctggacg tgaccttgat ggatatgccc
     1441 ctgagcagcc cggcatagcc tgaacggtaa ccgatgattt cggccttggg cgccaatttg
     1501 gtgtagtcca tgatcaggca gcccacggcg gcggaaaggc agggggcgag gcctccggcg
     1561 gtgagcatgg cgatttttgg ttttggcatg gacgactccc gaatccgcgc ccttcctaca
     1621 agacaaacta cggcgatgcc agccacgtcg tttggcctat aatgcggtat tgcagtgaca
     1681 gggggtttcg atgacatcac caaacatcga ggccgcacag cttctggcaa aacggctctt
     1741 aactttggaa cgaaggcgat gacggcccgc gaaaaacggg ggatcgagcg cgttgccaag
     1801 cgaaccgcag ttttgcgcga tagaaccggc tcgcggcggc gcatgactat gaagtgaatc
     1861 tcaaggcgga aatcgagatc atggcgctcc ataagaaatt gacgaaatcc gcgccaaggc
     1921 catcgccgtg tttctgaagc gcgctgtaaa gggcaaaaga tcgacgcctt aaaaatgagc
     1981 atactgccta ttttttagac atttagcttt tatgctccgt cgagtacatc ccctaaccca
     2041 ttgaaattat tgttttcagc ttgtggcaca ggtcgtgcaa tcgtttgagg acattaagaa
     2101 aaggctcaag catccatgaa aaaaatcgag gcgatcatca agcctttcaa gcttgatgag
     2161 gtgaaggaag cgctgcagga agtcgggctt ttgggaatta ccgtgaccga ggccaagggt
     2221 tatgggcggc agaagggcca cacggagctt t
//
LOCUS       DAORVN010000356         2245 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_49649_length_2245_cov_3.585845, whole genome shotgun sequence.
ACCESSION   DAORVN010000356 DAORVN010000000
VERSION     DAORVN010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2245
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49649_length_2245_cov_3.585845"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..670)
                     /locus_tag="PKV23_07585"
     CDS             complement(<1..670)
                     /locus_tag="PKV23_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016919007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HQX85333.1"
                     /translation="MSIYDVDLDKTPANYQPLTPLSFLARSASVYPDHTAIIHGKQRT
                     SYAGFYGRSRKMASALVKAGIKKGETVSAILANTPAMLECHYGVPMMGGVLNTLNTRL
                     DAATIGFSLDHAETKLLIVDKEFSSLAKNAIQLMKGPKPIVVDYVDPEFPVEGERLSS
                     IEYEQFIAGGDAEFEWLWPADEWEAISLNYTSGTTGNPKGVVYHHRGAYLMGSGNVLA
                     GNLPR"
     gene            complement(743..1873)
                     /locus_tag="PKV23_07590"
     CDS             complement(743..1873)
                     /locus_tag="PKV23_07590"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HQX85334.1"
                     /translation="MEMPAAAVLLRSGTLPANGFSGLLRQSISAHAIFIVIVAAYIAC
                     FVILLRLRPDIVPTSFLVATIGFVFLSIVFILLCTFIMRFYHIATIVKPERPIPALAK
                     DIKHYLANKKRMANGIPMVLIMMFFMYVFANLKAAIPVLNPLSWDTYFSDLDSTLHFG
                     TLPWVWLQPLLGYAPITFLININYNLWFLVTWMMWVHFAFADRPSELRTRFFLSFFAM
                     WVFIGGVLAIWFSSGGPCFFGRLGLTPDPYADLMAYLRGVNEVFPIWALPVQDELWQG
                     YVDNSVIVRGISAMPSMHNGSALLFALAGYKVSRFAGHLLSAHAILIFIGSIHLGWHY
                     AVDSYLAWALTLVIWFGVAPISRWWHTTAAQGDFDRALATAA"
     gene            2045..>2245
                     /locus_tag="PKV23_07595"
     CDS             2045..>2245
                     /locus_tag="PKV23_07595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQX85335.1"
                     /translation="MKTNTPAQASWATFTLFLLHGIMVGSWVPHIPLAKERLDVGPGV
                     FGLALLAIAAGAVTAMPLTGAMI"
BASE COUNT          548 a          645 c          631 g          421 t
ORIGIN      
        1 gcctgggcag gttccctgcc agtacattgc ccgaccccat gagataggcg ccccggtgat
       61 ggtagaccac gcccttggga tttcccgtgg tccccgaggt gtagttgagc gaaatcgcct
      121 cccattcatc ggcaggccaa agccattcaa attccgcgtc gccgccggca atgaactgct
      181 cgtattcaat cgagctcagc cgctcgccct ccaccgggaa ttccggatcg acatagtcaa
      241 cgacaatggg cttcggcccc ttcatcagtt gaatcgcatt cttcgcaagt gaggagaact
      301 ccttgtcaac aatcagtaat ttcgtttccg catggtccag ggaaaagccg attgtcgccg
      361 catcaagtcg ggtgttgagg gtgttgagaa ccccgcccat catcggcacg ccataatggc
      421 attccagcat cgccggcgta ttggcaagga tcgccgaaac ggtttcaccc tttttaatgc
      481 cggccttcac cagggcagag gccatcttgc gcgaccgccc gtaaaacccg gcatagctgg
      541 tgcgctgctt gccatggatg atggccgtat ggtcggggta gaccgaggcc gaccgggcca
      601 ggaatgagag gggcgtaagc ggctgatagt tggcaggcgt cttgtcgagg tcgacatcgt
      661 agatggacat ggaagcgcaa tccttgcggt gagaattgcc acatactgta aaaattcgca
      721 gctaaagtga agctaaactt tgttacgccg ccgttgccag ggcgcggtca aaatcgcctt
      781 gcgccgccgt tgtgtgccac cagcgtgaaa tcggtgccac gccaaaccag atcaccaggg
      841 taagggccca ggccagatag ctgtccaccg cataatgcca gccaagatgg atcgaaccga
      901 tgaatatcag gatggcgtgg gcactgagca gatggccggc aaaacggctg accttgtagc
      961 ccgccagcgc aaacagcagc gctgagccat tgtgcatgga tggcatggcc gaaatgcctc
     1021 ttacgatgac cgaattatca acatagcctt gccacagctc atcttgcaca ggcagggccc
     1081 aaatcggaaa tacctcgttc acgccgcgca gataggccat gagatccgca tatgggtcgg
     1141 gcgtgagtcc cagcctgcca aagaaacagg ggccgccgga tgaaaaccag attgccagaa
     1201 ccccgccaat gaagacccac atggcgaaga aactcaggaa gaaccgggtg cggagttcac
     1261 tggggcggtc cgcaaaagca aaatgcaccc acatcatcca ggtcaccagg aaccagagat
     1321 tatagttgat attgatgaga aatgtgatcg gcgcatagcc cagcagcggc tgcagccata
     1381 cccagggcag cgtgccaaaa tgcagggtgc tgtccaggtc ggaaaaatat gtgtcccatg
     1441 aaagcgggtt caacactgga atggccgcct tcaggtttgc aaaaacatac atgaagaaca
     1501 tcatgatcag gaccatggga atgccattgg ccatccgctt cttgttggcc agataatgct
     1561 tgatgtcctt tgccagtgcc ggaatgggcc gctccggttt cacaatcgtc gcaatgtgat
     1621 aaaagcgcat gatgaaagtg cagagaagta tgaaaacgat cgacagaaat acgaacccga
     1681 tggtggccac cagaaagctg gtgggaacaa tgtcggggcg caggcgcagc agaatcacga
     1741 agcaggcgat gtaggcagcg acaatcacga tgaaaatggc atgggccgaa atgctttgcc
     1801 gcaggaggcc ggagaacccg ttcgccggca aggtaccaga cctgagaagc acggctgcgg
     1861 caggcatttc catcaatcat cacctgaatg ttaaatcggc ggccaatatg gccggatcgg
     1921 ccttaaaatt ggttaagccc catcgctgag atcattcagc ccacgcaccc cgtccctgcg
     1981 caagcaatca ttggtatttg ccgcccccca cgctattcag gcccgaatcg caacgggaac
     2041 catcatgaaa accaacacac cggcccaggc cagctgggcc accttcaccc tctttttgct
     2101 gcacggaatc atggtgggct cctgggtgcc ccacatcccc ttggccaagg aacgccttga
     2161 cgtggggccc ggcgtctttg gcctggcgct tttggccatc gccgccggcg ccgtaaccgc
     2221 catgccgctg accggcgcca tgatc
//
LOCUS       DAORVN010000357         2236 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_50008_length_2236_cov_3.794131, whole genome shotgun sequence.
ACCESSION   DAORVN010000357 DAORVN010000000
VERSION     DAORVN010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2236)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2236)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2236
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50008_length_2236_cov_3.794131"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..696)
                     /locus_tag="PKV23_07600"
     CDS             complement(<1..696)
                     /locus_tag="PKV23_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HQX85336.1"
                     /translation="MFNQNLSQNPRILAIANQKGGVGKTTTAINLGTALAAVGETVLI
                     VDLDPQGNASTGLGIARGPKQKSSYHVLIGEAELDEVIVDSGIPRLHCVPSTIDLLGA
                     ELELADFDRKAYRLADAIARLTRQQNRARSYSYILVDCPPSLNLLTINSLSAADAILV
                     PLQCEFFALEGLSQLLKTVERVKTTLNPKLTIQGVVLTMFDRRNSLSDQVAQDVRSVL
                     GDKVYQTVIPRNVR"
     gene            complement(689..1321)
                     /gene="rsmG"
                     /locus_tag="PKV23_07605"
     CDS             complement(689..1321)
                     /gene="rsmG"
                     /locus_tag="PKV23_07605"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009338581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HQX85337.1"
                     /translation="MNGQYISDDELARLNVSRESLLRIETYVDLLLTWQSKINLIGPS
                     TIETVWRRHILDGLQLLPLLHAKTETIADLGSGAGIPGLILALGGNLRADLYESNGKK
                     VAFLREVIRQTKVDAQVHQIRLETLEAHLPERIPDYVTARALAPLDKLLAWAEPLLTR
                     GSIGLFHKGQDVDSEINKATKFWKMGAIIRHASMTDSDGTILEVKEITRV"
     gene            complement(1321..>2236)
                     /locus_tag="PKV23_07610"
     CDS             complement(1321..>2236)
                     /locus_tag="PKV23_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012113857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQX85338.1"
                     /translation="QEQFIRTIPGLENVAILRPGYAIEYDFVDPRELTSGLEVKALPG
                     LFLAGQINGTTGYEEAAAQGLIAGLNAARKVGGQAEISFDRAEGYLGVMVDDLVLRGV
                     TEPYRMFTSRAEYRLSLRADNADQRLTPKGISLGLVGAHRQKHFAQRMSVLNAARVLA
                     QNLGLTSAAAAKLGLKVNQDGQRRSALDLMAMPEVGLELVCEIWPEIAALPDFAKDGL
                     FADSLYAGYSARQSADILALRKEDQVSLPHDTDYMALPSLSIEIRQKLMRVRPGTLGQ
                     ASRIEGMTPAALACLLGHIKRQSLRRSA"
BASE COUNT          434 a          688 c          655 g          459 t
ORIGIN      
        1 acggacattg cgcggtatga ctgtctggta aaccttgtcg ccaagcacgc tgcggacatc
       61 ctgcgccacc tggtcggaaa ggctgtttcg ccgatcaaac atcgtaagaa caacaccctg
      121 aatggttaat tttgggttaa gagttgtctt cacccgctca actgttttca gtagctggct
      181 cagcccttcc agggcaaaaa actcgcattg caggggtacc agtatggcgt ctgccgccga
      241 gagggaattg attgtgagta gattgagcga cggcgggcaa tccaccagaa tatagctgta
      301 gctgcgggcc cggttctgct gccgcgtgag ccgggcgatg gcatcggcca gccggtaggc
      361 cttgcggtca aaatccgcca gctcgagctc ggcgcccagg agatcgattg tcgaaggcac
      421 gcagtgcagc cggggtatgc ccgagtcgac gataacctcg tccagttccg cctcgccgat
      481 gagcacgtgg taggacgact tctgcttggg cccgcgcgcg atcccaagcc ccgtgctggc
      541 gttgccttgc gggtccaggt cgacgatgag caccgtctcg ccaaccgcag cgagcgctgt
      601 tccgaggttg atggcggtcg tggttttacc caccccgccc ttctggttgg cgatggcgag
      661 gatccgggga ttttgcgaga gattctggtt aaacacgggt aatctccttt acctcaagaa
      721 tagtaccgtc cgaatcggtc atacttgcat gcctgatgat tgcgcccatt ttccagaatt
      781 tggtagcctt gttaatctca ctgtccacat cttgtccctt gtggaaaagg ccgatgctcc
      841 cccgcgtcag caacggctca gcccaggcca gcagcttgtc cagcggcgcc agggccctcg
      901 ccgtcacata gtccgggatc cgctccggca aatgcgcctc aagtgtctca aggcggattt
      961 gatgcacctg cgcatcgacc ttcgtctgcc gtatgacctc gcgcagaaag gcaaccttct
     1021 tgccgttgct ctcataaagg tcggccctga gatttcctcc cagcgccaga atcaggccag
     1081 ggattccagc gcccgaaccc aggtcagcga tagtttcggt tttggcatgc agcaatggca
     1141 gcagctgcag tccgtccaga atatggcgcc gccataccgt ctcgattgtc gaaggcccaa
     1201 tcaggtttat cttgctctgc caggtcagaa gcagatcgac ataggtttcg attctaagga
     1261 gtgattcacg tgaaacattc agccgtgcca gctcgtcatc ggaaatatac tggccattca
     1321 tcaggcactc cggcgcaggc tctggcgctt gatgtggccc aataggcagg cgagcgccgc
     1381 cggggtcata ccctcgatgc gcgaggcctg acccaaggtg ccgggcctga cccgcatgag
     1441 cttttgcctg atctcgatcg aaaggctggg cagggccatg tagtcggtgt catggggcag
     1501 gctgacctgg tcctccttgc gcagcgccag gatatccgcc gattgccggg cgctgtatcc
     1561 cgcataaagt gaatcggcga atagcccgtc cttggcaaag tccgggagcg ccgcgatctc
     1621 gggccagatc tcgcaaacca gctcgagccc tacttccggc atggccatga gatcaagcgc
     1681 cgagcgccgc tggccatcct gattgacctt taggccgagt ttggcggccg cggccgaagt
     1741 caaacccaag ttctgcgcca agacccgcgc cgcattcagg acgctcatgc gctgggcaaa
     1801 atgcttctgg cgatgggcgc caaccaggcc cagggaaatg cccttgggcg tcagtctttg
     1861 atcggcattg tcggcccgga gcgaaagccg gtattcagcc cgcgaggtga acatgcggta
     1921 gggctcggtc acgccgcgaa gcaccaagtc atccaccatg accccgagat agccttccgc
     1981 ccggtcaaag ctgatctccg cctggccgcc cacttttcgc gccgcgttca ggccggcgat
     2041 aaggccctgg gcggcggctt cttcatagcc ggtcgttcca ttgatctggc ccgccagaaa
     2101 caggccgggc agggctttga cttccagccc gctggtcagt tcacgggggt caacgaagtc
     2161 atactcgatg gcataccccg gccgcaggat cgcaacgttc tccaacccag gaatcgtccg
     2221 gataaactgc tcctgg
//
LOCUS       DAORVN010000358         2231 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_50214_length_2231_cov_2.584099, whole genome shotgun sequence.
ACCESSION   DAORVN010000358 DAORVN010000000
VERSION     DAORVN010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2231
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50214_length_2231_cov_2.584099"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..864)
                     /gene="serB"
                     /locus_tag="PKV23_07615"
     CDS             complement(<1..864)
                     /gene="serB"
                     /locus_tag="PKV23_07615"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019859004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HQX85339.1"
                     /translation="MASVVVLIAAPGSGAISAEVVAALRDHGAGNFHWLSPGDAAEVD
                     FFPRLGEFLIGVRSLPIDAAVVSTENRRKRLLVADMDSTMIHQECIDELGVMAGSGGL
                     IKDITARAMRGELDFEGALKARVALLRGLDQSVIRQVIRERITFMPGGKVLIATMKAN
                     GAYTALLSGGFTAFTSHVAGVLGFDEHRANTLVIANGKLTGTVTEPILGQEAKRQSLL
                     EIAVLRSLDAADAIAVGDGANDVAMLMEAGLGVALHAKPHVQESSMLRINHADLTALL
                     YLQGYRKDGQDL"
     gene            866..1723
                     /gene="miaA"
                     /locus_tag="PKV23_07620"
     CDS             866..1723
                     /gene="miaA"
                     /locus_tag="PKV23_07620"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011581080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HQX85340.1"
                     /translation="MNQTKAKSALLIAGPTASGKSALALALARERDGVIINADALQVY
                     KELRILSARPTDEELHMAPHRLYGHVSGLDSYSVAHWLADARREIESAWAAGKLPIIT
                     GGTGMYFMALEQGLAEVPSIDPEIREKWRSFEGDLYGELIRRDKESAKALKPNDRQRL
                     ARALEVIEGTGQPLSHWQAEAQSRAFLKDVAVERHVVEVPREELYNRAERRFDLMMAA
                     GALEEVRPLIKYGSVLPMMKAIGVPELTRHLRGEISLQEAVVLAKTATRQYIKRQLTW
                     WRGQAGRDH"
     gene            1974..>2231
                     /locus_tag="PKV23_07625"
     CDS             1974..>2231
                     /locus_tag="PKV23_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012484450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HQX85341.1"
                     /translation="MSGAEIVLKALADHGVEHVFGYPGGAVLPIYDEIFQQDKVKHVL
                     VRHEQGATHAAEGYARSTGKCGVVLVTSGPGATNAVTGLTDA"
BASE COUNT          431 a          639 c          747 g          414 t
ORIGIN      
        1 caggtcctgt ccgtccttcc ggtagccctg cagatagaga agggccgtca ggtcggcgtg
       61 gttgatgcgg agcatggagc tttcctggac atggggcttg gcatggagcg ccacgccaag
      121 gcctgcttcc atgagcatgg ccacgtcatt ggccccatcg cccacggcaa tggcatcggc
      181 cgcgtcgagg ctgcggagca cggcgatctc aagcagggac tggcgcttgg cctcctgccc
      241 cagaattggc tcggtgaccg tgccggtaag cttgccattg gcgatcacaa gcgtgttggc
      301 ccgatgctcg tcgaagccaa gcacgcccgc cacatgggag gtgaaggcag taaagccgcc
      361 ggagagcagg gcggtgtagg ccccgtttgc cttcatggtg gctatcagca ccttgccccc
      421 gggcatgaaa gtgatccgtt cacggatgac ctgccggatg accgactggt ccaggcccct
      481 gagcagggcg acgcgcgcct taagggcgcc ttcaaaatcg agttcgcccc gcatggcgcg
      541 ggcggtgata tccttgatga ggccgccgga gcccgccatc acgcccagct catcgatgca
      601 ttcctggtgg atcatggtgg aatccatgtc cgccacgagc aggcgcttgc gccggttctc
      661 ggtggaaacg acggcggcat cgatcgggag gctccgcaca ccgatgagga actcccccag
      721 gcgcgggaag aagtccactt cggcggcatc gccggggctg agccagtgga aattgccggc
      781 gccatggtcg cgcagggccg ccacgacttc ggcggagatt gcgccgctgc ccggggctgc
      841 tatgagaacg acaacggatg ccatgatgaa ccagaccaaa gccaaatctg cgctgcttat
      901 tgcaggcccc accgccagcg gcaagtctgc cctcgccttg gcgctggcaa gggagcggga
      961 tggcgtcatc atcaatgcgg atgcgctgca ggtttacaag gaactgcgca tactttcggc
     1021 ccggccgacc gacgaagaac tgcacatggc gccccaccgg ctttatggcc atgtgtcggg
     1081 gctcgacagc tattcggtgg cgcactggct ggcagatgcc aggcgtgaaa tcgaatcggc
     1141 gtgggccgcc ggcaagctgc ccatcattac gggcgggacc ggaatgtatt tcatggcgct
     1201 ggagcagggg ctggccgagg ttccgtccat tgacccggaa atccgcgaga aatggcggag
     1261 ctttgagggc gatctgtatg gcgagcttat ccgccgtgac aaggaaagcg ccaaggcgtt
     1321 gaagccaaat gaccggcaac gactggcgcg ggccctggag gtgatcgagg gaaccggaca
     1381 gccgctttcc cattggcagg cggaggcgca atcacgggcc tttctcaagg atgtcgccgt
     1441 cgagcgccat gtggttgagg tgccgcgcga ggaactttac aaccgggcgg aacgccggtt
     1501 tgacctgatg atggcggccg gggcgctgga agaagtgcgc ccgctcatta aatatggcag
     1561 cgtattgccc atgatgaaag cgatcggtgt tcccgaactc acccggcatt tgcggggtga
     1621 aatatccctt caggaagccg tggttctggc caaaacggcc acccgccaat acataaagcg
     1681 ccagctcacc tggtggcggg gccaggctgg ccgggaccac tgacagtcat aagcgattaa
     1741 cgaatttcaa ggggagcgct tgatttcttg gtccgggctg ggtagaagcg ctttatgaac
     1801 agcattctta ttgtcgtggg atcgcgtagc gccgcggagg gttaaccctc cgggataccc
     1861 ggtgttgcgc gaaacaggct ccttaagggg gcctttttta ttgcctgaat ggcaggttca
     1921 tgtgaaacga agtggctgga atgaagaggt ttgaaatggt tacgggcgaa ttgatgtcgg
     1981 gtgccgagat cgtgctgaag gctttggccg atcacggggt ggagcatgtc tttggctatc
     2041 ccggcggcgc ggtgctgccc atttacgacg agattttcca gcaggacaag gtcaagcacg
     2101 tgctggtgcg ccatgagcag ggggccaccc acgcggctga aggttatgcc cgttccaccg
     2161 gaaaatgcgg cgtggtgctg gtcacttccg gccccggtgc caccaatgcc gtcactggcc
     2221 tcaccgatgc g
//
LOCUS       DAORVN010000359         2228 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_50328_length_2228_cov_2.940635, whole genome shotgun sequence.
ACCESSION   DAORVN010000359 DAORVN010000000
VERSION     DAORVN010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2228
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50328_length_2228_cov_2.940635"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..981
                     /locus_tag="PKV23_07630"
     CDS             <1..981
                     /locus_tag="PKV23_07630"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HQX85342.1"
                     /translation="VADALERLGLPRIAATAVILLLSILALVLIVLLIVPILGDQIVK
                     FAGDLPSLMQSLLARFNDVAPQWMKDAIARSGTDIQASISDIAGRAAGWTATLLASIW
                     SGGMALVNILSLMVVTPIVAFYLLADWDRLVAKVDSWLPRDHAEEIRAIFNDINAAMA
                     GFIRGQGTVCLLLGLFYALALTVAGLKFGLVIGIGAGLLSFIPYVGALVGGVVAIGVG
                     LVQFWPDFTSIAIIIGIFAVGQFIEGNFLSPKLVGSSIGLHPVWLMFALFAFGYVFGF
                     VGLLLAVPLAAAAGVLVRFALNKYLGSKLYRGVPREMPTPRKTSVRNRAK"
     gene            978..1676
                     /locus_tag="PKV23_07635"
     CDS             978..1676
                     /locus_tag="PKV23_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019966534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85343.1"
                     /translation="MKGQGRQLVLDLPLRQALGRDDFLVTASNSAAVALIDHWPDWPA
                     HAAVIVGPPGSGKSHLVEVWRQRSKALRIQAVDVRVEAAPEILRSGAAAIEDAAPPLD
                     ERGLFHLLNLARQQGSSILLTAQMRPEHWKIALPDLLSRLKAFPVLEILPPDDALLRG
                     VLVKLFFDRQIAAAESTISFMLARMPRSLGAARLLVAEIDRRALEERAEVTRPFVAKV
                     LADFSAPGLFGAED"
     gene            1760..>2228
                     /locus_tag="PKV23_07640"
     CDS             1760..>2228
                     /locus_tag="PKV23_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009759899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA degradosome polyphosphate kinase"
                     /protein_id="HQX85344.1"
                     /translation="MNEMLPLEPAAAIGMDSPQRFFNRELSWLGFNMRVLEEARNRNH
                     PLLERLRFLSISGNNLDEFFMVRVAGLVGQVREQMVEISPDGLSPEEQLEKIRKFAQE
                     LMAEQDRRWLQLKSELTENGITIVNEEHLTADEHKWLDNRFLEHILPIVTPIAI"
BASE COUNT          377 a          685 c          662 g          504 t
ORIGIN      
        1 gtcgccgacg cgctggagcg cctcggtctg ccgcgcattg cggcaactgc cgtcattctt
       61 ctcctcagca tactggcgtt ggtgctgatt gttctgctca tcgttcccat ccttggcgac
      121 cagatcgtga agtttgcggg cgacttgccc agtctgatgc agtccttgct ggcgcgcttc
      181 aacgatgtgg caccgcaatg gatgaaggat gccatcgcca ggtccggaac cgatattcag
      241 gcttccattt ccgatatcgc tggcagggct gcgggctgga ccgcaaccct tttggcgtcg
      301 atctggtccg gcggcatggc gctcgtcaac atactttcgc ttatggtcgt gacgccgatt
      361 gtggcgtttt atcttctggc tgactgggac aggctggtgg ccaaggttga tagctggctg
      421 ccgcgcgatc atgccgaaga aatccgcgcc attttcaacg atatcaatgc ggcaatggcc
      481 ggattcatcc gcggccaggg aaccgtatgc cttctgctcg gcctgttcta tgcgctggca
      541 ctcactgttg ccggattgaa attcggcctg gtgatcggca ttggcgcggg actcttgagt
      601 ttcattccct atgtcggtgc gctggtgggc ggcgtggtgg caatcggcgt ggggctcgtc
      661 cagttctggc ccgattttac cagcatcgcg atcatcatcg gcatctttgc cgtaggccag
      721 ttcatcgagg gcaatttcct ctcgcccaag ctggtgggca gcagcattgg cctgcacccc
      781 gtttggctga tgtttgcgct cttcgccttc ggctacgtct ttggttttgt cgggctcttg
      841 ctcgccgttc cgctggccgc cgccgccggc gtgctcgtgc gctttgccct caacaaatac
      901 ctggggagca agctctatcg tggtgttccc cgggaaatgc caacacccag aaaaacctcg
      961 gtgcgtaacc gcgcgaaatg aagggccaag gccgacagct tgttctggac ctgcccctcc
     1021 ggcaagccct gggccgtgat gatttcctgg tgaccgcgtc caactctgcc gctgttgccc
     1081 tgattgacca ctggccggac tggcccgccc acgccgcggt gattgtcggc ccgccgggaa
     1141 gcggcaagtc ccatcttgtg gaggtctggc gccagcgctc aaaggcgctt cggattcagg
     1201 cagtggatgt aagggttgaa gccgcccccg agatattgcg ctccggcgcc gcggcaatcg
     1261 aggatgcagc accgcccctc gatgagcgcg gccttttcca tcttttgaac cttgccaggc
     1321 aacagggaag cagcattttg ctgacggccc agatgcggcc ggaacactgg aaaattgcct
     1381 tgcccgatct gctgtcgaga ctgaaagcct tcccggttct ggaaatattg ccgcccgatg
     1441 acgccctgtt gcgcggtgtg ctggtgaagc tgttttttga ccgccagatc gcggccgcgg
     1501 aatcaaccat cagcttcatg ctggcccgca tgccgcgctc ccttggcgcg gcccgcctgc
     1561 ttgtagcgga aatcgaccgc cgcgccctgg aagagcgcgc cgaggtgacc cgcccctttg
     1621 tggccaaggt tctggccgat ttctccgccc cgggcctctt tggcgccgag gactagcact
     1681 tcgtttaccc ccagcgcatt cgccgctttc accaaaccgt catatgggcc tattatccgt
     1741 tgaattttga ggattcccga tgaacgagat gctgcctctc gaacccgccg cggcgattgg
     1801 aatggatagc ccgcagcgtt ttttcaatcg cgagctgtcc tggctgggct tcaacatgag
     1861 ggttctcgaa gaggcccgca accgcaacca tcccttgctg gagcgcctgc gctttctctc
     1921 catttccgga aacaacctcg atgagttctt catggtgcgc gtggcgggcc tcgtgggcca
     1981 ggtgcgcgaa cagatggtgg agatcagtcc cgacggcttg agcccggagg agcagcttga
     2041 gaaaatccgg aaattcgccc aggagctgat ggccgagcag gatcggcgct ggctgcagtt
     2101 gaaaagcgaa ctgactgaga acggcatcac tattgtcaat gaggagcatc tgacagctga
     2161 cgagcacaag tggctcgaca accgcttcct cgagcacatc ctccccatcg tcacgcccat
     2221 tgccatcg
//
LOCUS       DAORVN010000360         2228 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_50333_length_2228_cov_2.697193, whole genome shotgun sequence.
ACCESSION   DAORVN010000360 DAORVN010000000
VERSION     DAORVN010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2228
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50333_length_2228_cov_2.697193"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..982
                     /locus_tag="PKV23_07645"
     CDS             <1..982
                     /locus_tag="PKV23_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009337189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin oxidoreductase family protein"
                     /protein_id="HQX85345.1"
                     /translation="VLLDLGARLGLPGMTRPDGTARYPGGYPDYIVNHERAPGLGPLA
                     GFRGKDGKSKGRGAVNPDQLKAYIDNGCHWFYELPDHMKYYRHVNWNYLDWSTFMGFM
                     GKPEPIIHQLYCEPLQKFRLAAQGHGKILPPEGHARRISTYFDPVPFWYPPFEGDMVS
                     NTEFPMHAITQRPMAMYHSWGAQNAWLRQILGRNWLYMNKDRAVSLGIAEGDWVTVTS
                     HHGQIKSQVKLMSGVNADTVWTWNAIGKRSGTWGLSADAPEAKKGFLLNHLISDLLPE
                     REGGYRFSNSDPVTGQAAWYDLKVRVEKAVDQSAESQPQFPVLRKLRGRK"
     gene            979..1782
                     /locus_tag="PKV23_07650"
     CDS             979..1782
                     /locus_tag="PKV23_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009337188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HQX85346.1"
                     /translation="MTSLPTPQPNQKKLGLVIDLDTCVGCQACVTSCKEWNTQGYSAP
                     LNDSDSYGADPSGVWLNRVHGYEVKEEGRDDTRVVHFPRSCLHCETPACVTVCPTGAS
                     YKRAEDGIVLVNPDTCIGCKLCSWACPYGAREYDYSHGVMKKCTLCVDRIYNTNLEPE
                     DRAPACVKACPTGARHFGDLGDPESDVSQLVKARDGFDLLPEMNYKPVNKYLPPRKRR
                     DAVTSAAIALPKGDGSAMDRLFSWADKFLSGKPAGTIASLQDMSDKLEG"
     gene            1787..>2228
                     /locus_tag="PKV23_07655"
     CDS             1787..>2228
                     /locus_tag="PKV23_07655"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008872182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HQX85347.1"
                     /translation="MHPAYSVILFTTASGAGYGLLALLGLVGFNHGPASSLIFGLTAM
                     VIALGLITIGLLSSTFHLGHPERAWRAFSQWRSSWLSREGVAAVLTYLPALLFAATWL
                     NIIHAPGWIGPLGLITAAMCAVTVFCTAKIYSALKTIRAWNHPLT"
BASE COUNT          430 a          728 c          619 g          451 t
ORIGIN      
        1 cgtcctgctg gacttgggcg ctaggcttgg ccttcccggc atgacgcgtc ccgatggcac
       61 ggcgcgctat cccggcggct atcccgacta catcgtgaac catgaacgcg cccctggtct
      121 tgggccgctc gcagggtttc gcggcaagga cggcaaatcc aagggcaggg gggccgtaaa
      181 tcccgatcag ctcaaggcct atatcgacaa tggctgccac tggttttacg aattgccgga
      241 tcacatgaag tattaccgcc atgtgaactg gaattatctc gactggtcta ccttcatggg
      301 tttcatggga aagccggaac ccatcattca ccagctctat tgcgagcccc tgcaaaagtt
      361 tcgcctggcg gcccagggcc acggaaaaat cctgccgccc gaaggacacg ccaggcgaat
      421 atcaacctat tttgatcccg tgcccttctg gtatccgcca ttcgaaggcg acatggtttc
      481 caacacggaa tttcccatgc acgccatcac ccagcgcccc atggccatgt accattcctg
      541 gggtgcgcag aatgcctggc ttcgccaaat cctgggccgc aactggctct acatgaacaa
      601 ggaccgtgcc gtttccctcg gcatcgccga aggcgattgg gtgacggtga cctcccacca
      661 cggccagatc aaatcccagg tgaagctcat gagcggcgtc aatgccgata cggtctggac
      721 ctggaatgcc atcggcaagc ggtccggcac ctgggggttg tccgccgatg cgccggaagc
      781 caaaaaagga ttcctcctca atcacttgat ctctgatctt ctcccggaac gcgaaggcgg
      841 ctaccgcttc tccaacagcg atcccgtgac ggggcaggcg gcgtggtatg atctcaaggt
      901 gcgcgttgaa aaggcggtgg accagtcagc ggaaagccag ccgcagttcc cggttttgag
      961 aaaactgcgg gggcgcaaat gacttcgctt ccaaccccac agcccaacca gaaaaaactc
     1021 ggcctcgtca tcgatctgga tacctgcgtc ggctgccaag cctgtgtcac ctcctgcaag
     1081 gaatggaaca cgcaaggcta ttcggccccg ctcaacgata gcgactccta tggcgctgat
     1141 ccgtccggcg tctggctgaa ccgcgttcac ggctacgagg tgaaggaaga gggcagggac
     1201 gatacccgtg tggtgcattt cccgcgctcc tgcctgcact gcgaaacccc ggcctgcgtc
     1261 accgtttgcc cgacaggtgc ttcctacaag cgcgccgagg atggcatcgt gctggtgaac
     1321 cccgacacct gcatcggctg caaactctgt tcctgggcct gcccctatgg cgcgcgtgag
     1381 tatgattaca gccatggcgt catgaagaaa tgcacgctct gcgtcgaccg catctacaac
     1441 accaatcttg aacccgaaga ccgtgctccg gcctgcgtga aagcctgccc gaccggggct
     1501 cgccacttcg gcgatctggg cgaccctgag tccgatgtct cgcaactggt caaagcgcga
     1561 gacggcttcg atctgctgcc cgagatgaac tacaagccgg tgaacaaata ccttccgccg
     1621 cgcaagcgcc gggatgcggt gacgtctgcc gctatcgcct tgcccaaggg cgatggctcg
     1681 gccatggacc ggctcttctc ctgggccgac aagttcctga gcggcaagcc cgccggcacc
     1741 attgcctcgc tccaagacat gtccgacaag ctcgaaggct gacgccatgc atcccgccta
     1801 ttcggtcatt ctgttcacta cggcgtcagg cgcaggctat gggctgctcg ccttgcttgg
     1861 tttggtcggc ttcaaccatg gccccgcctc aagtctcatc tttggattga cggccatggt
     1921 catcgccctc ggcctgatca ccatcggcct gctgtcctcg acctttcatc tcggccaccc
     1981 ggaacgcgcc tggcgggcct tctcccaatg gcgtagttca tggctctcgc gcgaaggtgt
     2041 ggccgcggtt ttgacttatc tgcccgcgct attgttcgcg gcgacatggc tgaatatcat
     2101 tcatgcgccc ggctggattg gcccgctggg cctcatcacc gcggccatgt gtgccgtcac
     2161 cgttttctgc accgcaaaaa tctactctgc tctgaaaacc atccgcgcct ggaatcaccc
     2221 gcttactg
//
LOCUS       DAORVN010000361         2226 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_50402_length_2226_cov_3.286043, whole genome shotgun sequence.
ACCESSION   DAORVN010000361 DAORVN010000000
VERSION     DAORVN010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2226
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50402_length_2226_cov_3.286043"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..763
                     /gene="dapE"
                     /locus_tag="PKV23_07660"
     CDS             <1..763
                     /gene="dapE"
                     /locus_tag="PKV23_07660"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF009557.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HQX85348.1"
                     /translation="ITGDEEGPAINGTIKVLEWMKANGHIPDHCLVGEPTCVAKLGDT
                     IKIGRRGSLSFIVSVAGVQGHVAYPQNTDNPIPKLARFLDRISFAKIDDGNDHFDPSS
                     LAVTTVDVGNPAGNVVPARATAKFNIRYSTEHDYESLRQWVDAQVASVRDELGGTWTV
                     TSHEGAEAFITEPGAFVGLVQDAVEQETGLVPKLSTSGGTSDARFIKNYCPVLEFGPT
                     NATSHKVDEHISVEELRATQVIYSRVIAAYFNNQP"
     gene            complement(747..1484)
                     /gene="truA"
                     /locus_tag="PKV23_07665"
     CDS             complement(747..1484)
                     /gene="truA"
                     /locus_tag="PKV23_07665"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_038966077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HQX85349.1"
                     /translation="MPRYKLTLEYNGAEFYGWQIQLHDETVQGVLTAAIAQINGAPAM
                     VQGAGRTDAGVHALGQVAHVDLLREWDPFVLRNAINAGVRPHKVSVLEAELVPDTFNA
                     RFSGTKRHYLYRILNRRAPPTLDLGQVWFVPVELDADAMHEAAQALVGHHDFSTFRDS
                     QCQAQSPMRTLDRLDVARFGEFIEIRTESRSFLHSQVRSMVGSLKLVGDGKWNVADMK
                     AALEARDRTACGAVAPPEGLYLIRVDY"
     gene            complement(1484..>2226)
                     /gene="fmt"
                     /locus_tag="PKV23_07670"
     CDS             complement(1484..>2226)
                     /gene="fmt"
                     /locus_tag="PKV23_07670"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HQX85350.1"
                     /translation="DYAASQGFTIRTPLSLKSADAQKAFADLNADAAVVVAYGLLLPQ
                     AILDAPRFGCFNVHASLLPRWRGAAPIQRAIMAGDAQSGVTIMRMELGLDTGPMMKSG
                     RVPISPETTASSLHAELAVLGAKLMVEVLQHPENPGTPQPAEGITYAKKIDKAETKID
                     FTKPAVEVRNHIHGLSPFPGAWCSLAGARVKILKAQAVDHAGDEALTFACGSGAIKFL
                     LLQREGKGVMDAATFLRGFPLAPETKPA"
BASE COUNT          447 a          703 c          659 g          417 t
ORIGIN      
        1 cataacgggc gacgaggaag gtcccgccat taacggcacc ataaaggtcc tggaatggat
       61 gaaagccaat ggccacatcc ccgaccattg cctggtgggg gaacccacct gcgtggccaa
      121 gctcggcgac accatcaaga tcggccgccg cggctccctg agttttatcg tgagcgtcgc
      181 cggcgttcag ggccatgtgg cctacccgca gaataccgac aatccgattc caaaattggc
      241 gcgctttctg gaccggatct cctttgcgaa aatcgatgac ggcaatgacc atttcgatcc
      301 gtcgagcctg gcggtgacca ccgtcgacgt gggcaatccg gcgggcaatg tcgtgcccgc
      361 ccgcgccacg gccaagttca acatccgcta cagcaccgaa catgattacg agtcgctgcg
      421 ccagtgggtc gatgctcagg tggcaagcgt cagggacgaa ctcggcggaa cctggaccgt
      481 cacctcccat gaaggtgctg aagctttcat caccgagccc ggcgcctttg tcggcctcgt
      541 gcaggatgcg gtggagcagg aaaccgggct ggtgcccaag ctttcaacct ccggcggcac
      601 ttcggacgcg cgcttcatca agaactattg ccccgttctg gagttcggcc ccaccaatgc
      661 aacttcccac aaggttgacg agcacatatc ggttgaagaa ctgcgcgcca cgcaagtgat
      721 ctacagccgg gtcatcgccg cctatttcaa taatcaaccc tgatgagata aagcccttcc
      781 ggcggcgcga cggcgccgca ggccgtgcgg tccctcgctt caagggctgc cttcatatcg
      841 gcaacattcc atttgccgtc acccaccagc ttcagcgaac ccaccatgga acgcacctgg
      901 gaatgcagga acgaacgcga ctccgtgcgg atttcgatga attcgccgaa gcgcgccacg
      961 tcgaggcggt ccagcgtacg catcggcgac tgcgcctggc actggctgtc gcggaaggtt
     1021 gaaaaatcat ggtggcccac cagcgcctgt gccgcctcgt gcatggcatc cgcatcgagt
     1081 tccaccggca caaaccacac ctggccgaga tcaagggtgg gcggcgcccg gcggttcaga
     1141 atgcggtaga gataatgccg tttcgtgccg gaaaaacggg cattgaacgt gtccggcaca
     1201 agctccgcct caagaacgga aaccttgtgc ggcctcacgc ctgcattgat cgcattgcgc
     1261 aggacaaagg ggtcccattc gcgcagcaag tccacatgcg cgacttgccc cagtgcgtgc
     1321 acgcccgcgt cagtgcggcc cgccccctgc accatggcgg gcgccccgtt gatctgcgcg
     1381 atggccgccg tcagcacgcc ctggaccgtc tcgtcatgga gctgaatctg ccagccgtag
     1441 aattcagcac cgttgtattc gagggtgagc ttgtagcgcg gcatcaggcg ggctttgtct
     1501 ctggggcgag gggaaagccg cgcaggaatg tcgccgcatc catcacgcct ttgccttccc
     1561 gctgcagcag gaggaatttt atggccccgc tgccgcacgc aaatgtcagt gcctcatccc
     1621 cggcatgatc gacagcctgc gccttgagaa ttttcacccg cgccccggcg aggctgcacc
     1681 aggcgccggg aaacggcgac agcccgtgaa tatgattgcg cacctctact gctggtttgg
     1741 tgaaatcaat tttcgtctcg gccttgtcga tttttttcgc gtaagtgata ccttcagcgg
     1801 gctgaggcgt tcccgggttt tcaggatgct gcaggacctc aaccatcagc ttcgcgccga
     1861 gtactgccag ttcggcatgc aaagaggaag ccgtggtttc agggctgatg ggaacacggc
     1921 ccgatttcat catcggaccc gtgtccagtc ccagttccat gcgcatgatc gtgacgcctg
     1981 attgcgcatc gcctgccatg atggcgcgct ggatgggtgc ggcccctcgc cagcgcggca
     2041 gcagcgaggc gtgaacattg aaacagccaa agcgtggtgc atccagaatc gcctgcggca
     2101 ggagcagacc ataagcaacc acgaccgccg cgtcggcgtt gagatcagca aaggcttttt
     2161 gcgcatcggc ggatttcagg gagagcggcg ttcgaatcgt aaagccctgt gacgccgcat
     2221 agtcct
//
LOCUS       DAORVN010000362         2225 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_50436_length_2225_cov_3.634101, whole genome shotgun sequence.
ACCESSION   DAORVN010000362 DAORVN010000000
VERSION     DAORVN010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2225
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50436_length_2225_cov_3.634101"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..961
                     /locus_tag="PKV23_07675"
     CDS             <1..961
                     /locus_tag="PKV23_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019171876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HQX85351.1"
                     /translation="DTLTWVLYEKELGRANYALHWNCVARPSNILMACVGEQREKYLF
                     PAVQGNMVDCLAMTEPGVGSDVRGMKCFAKQDGDDFVINGTKHFISHADEATFTILFA
                     ATGEEDTPRGKKKLITAFLVDKGTKGFTVRDGYKNVSHRGYNNCILEFNDCRLHKSQI
                     LGELHKGFEVANEWLGATRLQVASTCLGRAERAMEHAKQYAVDRYQFGQQIGKFQGVS
                     FKLADMAMELKAAELLTLEAAWKFDQKTVTDMDMAMAKLKATEVLAFIADEAIQIHGG
                     MGLMSDLPLERIWRDARVERIWEGTSEIQRHIISRALLRPLGG"
     gene            964..>2225
                     /locus_tag="PKV23_07680"
     CDS             964..>2225
                     /locus_tag="PKV23_07680"
                     /inference="COORDINATES: protein motif:HMM:NF024994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HQX85352.1"
                     /translation="MTRSLTALLNPKSIAFIGGTECDVAIRRTRELGFPGKIWALHPK
                     RESLGGIACVRSVAEIEGALDAAFVAIKREPTIEVVRELRKKGCGGAVIYASGFAETG
                     DAHLQKELLEAADGMPLMGPNCYGFVNCLARVAPWPDEHGMEIIDRGVAIITQSGNIA
                     CNFTFTRRALPLACIFAIGNQADIDMAQLLEELARDPRITAIGLHIEGLTDVAAFARA
                     AEVARKLKKPVIALKTGRSEQGAKVAMSHTSSLAGADTLYDALFERYGIARMKSVTSF
                     VETLKFLHHGGPLADSRIVSMSCSGGEAALIADMALERKVSFPPFDPKTKAEVKATLN
                     EYVSIDNPLDYHTFIWDQFDNLTATYSAVLRGGFDAGVLILDAPTHKGSNAAAWVVAA
                     KALAQAARNTGARAVVTASLPECMLPEVA"
BASE COUNT          470 a          711 c          624 g          420 t
ORIGIN      
        1 cgatacgctg acctgggttc tctacgaaaa ggaattgggc cgcgccaact acgcgctgca
       61 ctggaattgc gtcgcccgcc cctcaaatat cctcatggcc tgcgtcggtg agcagcgcga
      121 aaagtacctc tttcctgccg ttcagggaaa tatggtcgat tgcctcgcca tgaccgagcc
      181 gggcgttggc tcggacgtcc gcggcatgaa atgctttgcc aaacaggatg gcgatgattt
      241 cgtgatcaat ggcaccaagc atttcatcag ccatgccgac gaagccacct tcacgattct
      301 cttcgccgca accggcgagg aagacacacc gcgcggcaag aaaaaactga tcaccgcctt
      361 ccttgtggac aagggcacca agggcttcac cgtgcgtgac ggctacaaga atgtctccca
      421 ccgcggatat aacaactgca tcctcgaatt caacgattgc cgcctgcaca aatcgcagat
      481 tctcggtgag ttgcacaagg gcttcgaagt cgccaacgaa tggctgggcg ccacccggct
      541 gcaagtcgcg tcaacctgtt taggccgtgc cgaacgcgcc atggaacatg ccaagcaata
      601 tgccgtggac cgctaccagt tcggccagca gatcggcaag ttccagggcg tgtccttcaa
      661 gctcgccgac atggccatgg aactgaaagc cgctgaactg ctcaccctcg aggccgcctg
      721 gaagttcgac cagaaaaccg tcactgacat ggacatggcc atggccaagc tgaaggccac
      781 tgaagtgttg gccttcattg ccgatgaggc cattcaaatc cacggcggca tgggcctgat
      841 gtctgatctc ccgctggagc gcatctggcg cgatgcaagg gtcgagcgca tctgggaagg
      901 cacctccgag atccagcgcc acatcatttc ccgcgccctg ctgcgccccc tgggtggctg
      961 atcttgacgc gtagcctcac tgccctgctc aaccccaagt caatcgcctt catcggcggc
     1021 accgaatgtg atgtcgccat ccgccgcacg cgcgaactgg gctttcccgg aaagatctgg
     1081 gccctccacc ccaagcggga aagtctcggc ggcattgcct gcgtcagatc cgttgccgaa
     1141 atcgaaggcg cgctcgatgc cgctttcgta gccatcaagc gcgaaccgac aatcgaagtg
     1201 gtccgtgaac tgcggaagaa gggctgtggc ggagcggtga tctacgccag cggtttcgcc
     1261 gaaacaggcg atgcccacct ccagaaggaa ctgcttgaag cggcggatgg catgccgctc
     1321 atggggccca attgctacgg cttcgtgaac tgcctcgccc gcgtcgcccc ctggccagac
     1381 gaacacggca tggaaatcat cgacaggggc gttgccatca tcacccagtc gggcaacatc
     1441 gcctgcaatt tcacgttcac ccgccgcgcc ctgccacttg cctgcatctt tgccatcggc
     1501 aaccaggccg acattgatat ggcgcaattg cttgaggaac tcgcccgcga cccgcgcatc
     1561 acggcgatcg gcttgcatat tgaaggccta actgacgtgg cagcctttgc ccgtgccgcc
     1621 gaggttgccc gcaagctcaa gaagccggtt atcgccctga aaaccggccg ttcggagcaa
     1681 ggcgcaaaag tggcaatgag ccacacctca tcgctcgccg gcgccgacac cctctatgac
     1741 gcactgttcg agcgttacgg catcgcccgc atgaagtcgg tgacgtcatt tgtggaaacc
     1801 ctgaagtttc tccaccacgg cgggcccttg gctgacagcc gcattgtgtc catgagctgt
     1861 tcgggaggcg aagccgccct catcgccgat atggcgctgg agcggaaggt gagcttcccg
     1921 ccatttgacc cgaagacaaa ggcggaggtg aaggccaccc tcaatgaata tgtctcaatc
     1981 gacaacccgc tcgactatca caccttcatc tgggaccagt tcgacaattt gaccgcgacc
     2041 tattcagccg tgctgcgtgg cggctttgat gcaggcgtgt tgattctgga tgcaccgaca
     2101 cataagggaa gcaatgccgc cgcctgggtc gttgcggcaa aggcgcttgc ccaagccgct
     2161 agaaacaccg gcgcacgcgc cgttgttact gcctcgcttc ccgaatgcat gcttcccgaa
     2221 gttgc
//
LOCUS       DAORVN010000363         2217 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_50804_length_2217_cov_2.666512, whole genome shotgun sequence.
ACCESSION   DAORVN010000363 DAORVN010000000
VERSION     DAORVN010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2217
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50804_length_2217_cov_2.666512"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..594)
                     /locus_tag="PKV23_07685"
     CDS             complement(<1..594)
                     /locus_tag="PKV23_07685"
                     /inference="COORDINATES: protein motif:HMM:NF020037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HQX85353.1"
                     /translation="MARAFDPASDFRALFEGSPNPYLVLDPHFKIVAVNDAYCQATMT
                     NRKTILGRDIFDVFPDNPADLEATGVGNLRASLERVLQFRCPDTMAIQKYDIPRPASE
                     GGGFEERYWSPLNSPVLDGRGKVSWIIHRVEDVTERWKTEDALKQESAFYDIVIDNIP
                     AMVFVKDAVDLRFVLINRAGEELLGIDRSEFIGKSDYD"
     gene            733..987
                     /locus_tag="PKV23_07690"
     CDS             733..987
                     /locus_tag="PKV23_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HQX85354.1"
                     /translation="MDLVGILIFLLIGAIAGWLAGQIMKNGSFGLLGDIIVGIVGSAI
                     ASFILPRLGIMIGGGVLGAIINATIGACILLFVLRLVRRG"
     gene            992..1138
                     /locus_tag="PKV23_07695"
     CDS             992..1138
                     /locus_tag="PKV23_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015315688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85355.1"
                     /translation="MSRNLLYAITAAFGVAVVILGYMVYEDRKKPDGVEIKIDDNGIS
                     IQGN"
     gene            1235..2086
                     /locus_tag="PKV23_07700"
     CDS             1235..2086
                     /locus_tag="PKV23_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008874221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HQX85356.1"
                     /translation="MKTGFNLLLWTTHVTAEHTAILKGIRKAGYDTVEIPVFEGTPEH
                     FARLGGVLDKLGLERSTVSVIGPSGKNPLAADAKARTAAVDYTKWVIDCTHALGARTI
                     VGPMHSEIGHFSGDGPTAAEIKRGISFHRKVGDYAAKKDVRFALEAINRFECYFINSM
                     QQLADYLDEVDHPAIKGMYDTFHANIEEKDPVAAIKTIRKHMIHVHISENDRGTPGKG
                     HVPWDATYKALRKAKYDGWMTIEAFGRALPALAAATRVWRDFFPSQEEVYKFGYKSMK
                     QGWAKAK"
     gene            2083..>2217
                     /locus_tag="PKV23_07705"
     CDS             2083..>2217
                     /locus_tag="PKV23_07705"
                     /inference="COORDINATES: protein motif:HMM:NF018498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tm-1-like ATP-binding domain-containing protein"
                     /protein_id="HQX85357.1"
                     /translation="MKTVYVIGTCDTKEAELRYAVERVKAAGAMALLVDISTTPSSAA
                     A"
BASE COUNT          488 a          596 c          690 g          443 t
ORIGIN      
        1 atcgtaatcg gacttgccga tgaattcgct gcggtcgatg cccaaaagct cctcgccggc
       61 gcggttgatg agtacaaagc ggagatccac ggcatccttc acgaagacca tggcaggaat
      121 gttatcgatc acaatatcgt agaaggcgct ttcctgcttc agggcatcct cggtcttcca
      181 gcgctcggtg acatcctcga cccggtggat gatccaggag accttgccgc ggccgtcaag
      241 cacaggcgaa ttgagcgggc tccagtagcg ttcctcgaaa ccgccgccct cggaggcggg
      301 gcggggaatg tcgtatttct gaatagccat cgtgtcggga cagcggaact gcaggacgcg
      361 ttcgagcgaa gcgcgcaggt tgcctacgcc ggtggcctcc agatcagccg gattgtccgg
      421 gaagacatca aagatatcgc gtcccagaat cgtcttgcgg ttggtcattg tcgcctggca
      481 ataggcatca ttgacggcga cgatcttgaa atgggggtca agcacaagat agggattggg
      541 agaaccctcg aacagggccc ggaagtcaga tgccggatca aaggctcgtg ccatttgctt
      601 gattccatgc aattgcgccg gtgagcttac aggcaaaccg caaactggga aaacttgcga
      661 aggcggtcga atcggaccaa tgcaatgagg ttggcatatt tgccgtggca ttcaaatctg
      721 cgggaggcaa aaatggatct ggtcggaatt ctcatctttc tgttgattgg cgcgatcgcc
      781 ggctggcttg ccggccaaat catgaagaac ggcagcttcg gcctgcttgg cgacatcatc
      841 gtcggcattg tcggttcggc gattgccagt ttcatcctgc cgaggctcgg aatcatgatt
      901 ggcggcggcg ttcttggggc aatcatcaat gccaccatcg gcgcctgcat attgctgttc
      961 gtactccgcc tggtcaggcg tggctgaaac gatgagccgc aatctgcttt acgccatcac
     1021 agcggctttt ggcgtggccg tcgtgatttt gggctacatg gtctatgagg accggaagaa
     1081 gcccgacggc gttgaaatca agatcgacga caacgggatt tccatccagg gtaactgacc
     1141 cgggcgctga ccagcggctg accggcgcat atcaaagttt gacaaaaccc tccggaaagg
     1201 ctgtcttaat tgtctttgtt ttgaggggct tgcgatgaaa accggattca acctgctgct
     1261 gtggacgacg catgttacag ccgagcacac ggccattctg aagggcatca ggaaggcggg
     1321 ttatgacacg gtggaaatcc cggtgttcga gggcacgcca gagcattttg ccaggctggg
     1381 cggggtgctg gacaagctgg ggcttgagcg cagcaccgtg agcgtgatcg ggccttccgg
     1441 caagaacccg ctagcggcgg atgcaaaggc gcgcacggcg gcggtcgact acaccaaatg
     1501 ggtcattgat tgcacccatg cgcttggcgc caggacaatc gtcgggccaa tgcattcgga
     1561 gatcgggcac ttttccggcg atggtcccac cgctgccgaa atcaagcgtg gcatttcctt
     1621 ccaccgcaag gtgggcgact atgcggcaaa gaaggacgtg cgctttgcgc tcgaggcgat
     1681 caaccgcttt gaatgctatt tcatcaacag catgcagcaa ctcgccgact atctcgatga
     1741 agtggaccat ccggcgatca aaggcatgta tgacacgttc catgccaata tcgaggagaa
     1801 ggatccggtg gcggccatca agaccatcag gaagcacatg atccatgtgc atatatccga
     1861 gaatgaccgg ggtacgccgg gcaagggaca tgtgccgtgg gacgcaacct acaaggcact
     1921 gcgcaaggcc aagtatgacg ggtggatgac catagaggca tttggccgcg cgcttcccgc
     1981 acttgcggcg gcgacgcggg tgtggcgcga tttctttccg tcccaggagg aagtctacaa
     2041 gttcggctac aagagcatga agcagggctg ggccaaggcg aagtgaagac cgtttacgtc
     2101 atcggaacct gcgacaccaa ggaggcggaa cttcgctacg cggtggagag ggtcaaggcc
     2161 gccggggcca tggccctgct ggtcgacatt tccaccacgc ccagcagtgc tgcggcc
//
LOCUS       DAORVN010000364         2215 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_50873_length_2215_cov_3.699074, whole genome shotgun sequence.
ACCESSION   DAORVN010000364 DAORVN010000000
VERSION     DAORVN010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2215
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50873_length_2215_cov_3.699074"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2215
                     /locus_tag="PKV23_07710"
     CDS             <1..>2215
                     /locus_tag="PKV23_07710"
                     /inference="COORDINATES: protein motif:HMM:NF017930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HQX85358.1"
                     /translation="YDMLASEARLASFLAIAKGDLRTRHWFRLGRTVTAVKGGAALVS
                     WSGSMFEYLMPSLVMRAPGAGLLDQTTRLVVLRQIAYANSLGLPWGISESAFSARDIE
                     LTYQYSNFGVPGLGLKRGLADNTVIAPYATGLAAMVSPRSAAKNFERLAREGARGDYG
                     YYEALDFTPARLRKGETVAIVRAYFAHHQGMTIVAILNAVKNGEMRNHFHAEPMVRAT
                     ELLLQERAPRNVPLTHARAEAAAGGDAVRDIVSPTARIYSMKGSASPATHLLSNGQYS
                     VMTTAAGSGYSMWNGLALSRWREDGVCDDWGSFIYLREPKSGKVWSAGHMPVAANADS
                     YTAAFTEDKAEITRIDGYFTTTLECTVSPEDNAEVRRVTIANTGLAAREIELTSYTEL
                     VLTQAAADTAHPAFSKMFVETEYLADLETLLATRRRRSPGDPEIWIAQFMLIQGAAVG
                     MPEVETDRARFIGVGNSTHMPAAMKSSERLSNTVGAVLDPIFAIRRRMRIPAGRQVRC
                     TIWTVVADTREGVLDLVDRHRQPTAYDRALMLAWTQAQIQLRHLSIDTEEAHLYQSLA
                     SHLIYANPALRAPGKILLQGMGPQSALWPQGISGDSPILLVRIDDATDIELIRQLLHA
                     FEYWKTKRLKVDMVILNDRASSYAQDLQGTIEALVRKISTPRTAGGPGDMGQVFTLRA
                     DLLSQETLRVLPAVARVVLYGRRGDVASQLPRSRDTSAATSVQHNEPPRLLPAPKA"
BASE COUNT          447 a          681 c          659 g          428 t
ORIGIN      
        1 tatgacatgc tggcctccga agctcggctg gcaagcttcc ttgccattgc aaagggcgat
       61 ctgcggaccc gccattggtt caggcttggg cgtaccgtaa cagcggtcaa aggtggcgct
      121 gcgctcgtct cctggtcggg ttcgatgttc gagtatctca tgccctctct cgtcatgcgc
      181 gctcccggcg ccgggctgct tgatcaaacg acacggctgg ttgtcctgcg gcagattgcc
      241 tatgccaatt ccctcggcct gccatggggc atttcagagt cagctttcag cgcgcgcgac
      301 atagagctta cctatcagta ctcaaacttc ggtgtaccag gccttggcct caaacggggc
      361 ctggcggaca acacagtcat tgcaccttat gcaaccggcc tggcagccat ggtcagtccc
      421 cgctctgcgg caaaaaactt tgaacgcctg gcgcgcgagg gcgcgcgggg ggactacgga
      481 tactacgagg cgctggattt cacgccggcg cgattgcgca agggcgagac cgtggccatc
      541 gtgcgggcct attttgccca ccatcagggc atgacgattg ttgccattct gaatgccgtg
      601 aaaaacggcg aaatgcgcaa tcactttcat gccgagccca tggtgcgcgc aaccgaactg
      661 ctgctccagg aacgtgcgcc gcgcaatgtg cccctgaccc atgcgagggc ggaagccgcc
      721 gccggcggcg acgcggttcg cgacatcgtt tcccccacgg cgcgcatata cagcatgaag
      781 ggctccgcct cacccgccac ccatttgctt tcgaacgggc aatacagcgt catgactacc
      841 gctgcaggct ccggctacag catgtggaat ggcctcgccc tcagccgctg gcgcgaagac
      901 ggggtttgtg atgattgggg atctttcatc tatctgcgtg agccgaaaag cggcaaggtc
      961 tggtccgcgg gccatatgcc agttgccgcc aacgcggaca gctacacagc cgcatttacc
     1021 gaggacaagg ccgagatcac gcgtattgac ggctacttta caacgacgct ggaatgcacc
     1081 gtgtcacccg aggacaatgc cgaggtgcgg cgcgtaacca ttgccaacac gggattggca
     1141 gcccgggaaa tcgaactcac gtcatacaca gaactggtgc tgacgcaggc tgctgcggat
     1201 acggcccacc cggctttctc caagatgttc gttgaaaccg agtacctggc tgatctcgaa
     1261 acactgttgg ctactcgccg ccgccgttcg ccaggcgatc ctgaaatctg gatcgcgcaa
     1321 ttcatgctca tccagggcgc ggctgtagga atgccggagg tggaaaccga cagggcacgc
     1381 ttcattggtg tgggaaacag cacgcatatg ccagcggcga tgaaaagcag cgaacgcctt
     1441 tcgaatacgg tgggcgctgt tctcgatccc attttcgcca tccgccgccg catgcggatt
     1501 cctgccgggc gacaggttcg ctgcaccatt tggaccgttg ttgcggacac acgggaaggt
     1561 gtccttgatc tggttgaccg gcaccggcag cctaccgcct atgaccgggc gctcatgctg
     1621 gcgtggacgc aggcgcaaat acagctgcgg catctgtcga tcgacactga agaggcccat
     1681 ctctaccagt ctctggcgag ccatcttatt tatgcaaatc cagcgcttcg ggcgcctgga
     1741 aagatcctgc tgcaaggcat gggaccccaa tcggcactct ggccacaagg catttcaggt
     1801 gactcgccaa tacttttggt tcgcatcgac gacgcgactg acattgaact catacgccag
     1861 ctcttgcacg cctttgaata ctggaaaacc aaaagactga aagtggatat ggtcatcctc
     1921 aatgaccgtg cctcctcgta cgcacaagac ttgcaaggaa cgattgaagc gctggtgcga
     1981 aaaatcagca cgccgcggac ggcgggcgga cctggcgata tgggacaggt attcacgttg
     2041 cgcgccgacc tgctgtcgca ggagacgctg cgcgtgctgc cggctgtagc gagggtggtg
     2101 ctgtacggcc gccgaggcga cgttgcgagc cagcttccaa ggtcgcggga tacgtccgcc
     2161 gccacctccg tccagcacaa cgaaccgccg cggctcctgc cagcgccgaa ggctg
//
LOCUS       DAORVN010000365         2214 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_50910_length_2214_cov_3.750811, whole genome shotgun sequence.
ACCESSION   DAORVN010000365 DAORVN010000000
VERSION     DAORVN010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2214
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50910_length_2214_cov_3.750811"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..496)
                     /locus_tag="PKV23_07715"
     CDS             complement(<1..496)
                     /locus_tag="PKV23_07715"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019859179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQX85359.1"
                     /translation="MQSHAKVVVIGGGVVGCSILYHLAKLGLNDVVLLERKELTSGSS
                     WHAAGIIHTINSDSNIARLQGYTINLYKELEELTGQSCGIHRPGGIYLASTRERYDYL
                     KQERAKARYLGLDSDFISLEHARELNPLIDPKKYVGALFEPVDGHVDPSGVTHAYAKG
                     ARHYG"
     gene            complement(649..1860)
                     /locus_tag="PKV23_07720"
     CDS             complement(649..1860)
                     /locus_tag="PKV23_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019171412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQX85360.1"
                     /translation="MATVENRIASNTVFAVLISVTVCHLINDTLQALMLAIYPILRDS
                     YALTFAQIGIITMVFHVTASILQPLIGLYTDRRPLPYSLPLAPAFTLLGVLLLAFATS
                     YAAILVAAAAVGIGSAIFHPEASRVARIASGGRFGMAQSLFQVGGNVGSSLGPLLAAW
                     IILPRGQASLVWFGVIALAAVGILSGVGRWYSQHLRPTHTSRPVSAIGHNLGSRAVSL
                     SLLILLALMFSKFVYTSSFSSFYTFYLIDHFQVPVKDAQIYLFVYLGAFAIGTFAGGP
                     IGDRIGRKAVIWFSILGALPFTLALPFANLFWTVVLTVPIGLILASAFSAMVVYAQEL
                     LPGRVGMISGLFFGTAFGIAGLSAVPLGYLADHTSITFVFQVCSVLPAIGLLTIFLPG
                     TERMKGQPSSP"
     gene            1997..>2214
                     /locus_tag="PKV23_07725"
     CDS             1997..>2214
                     /locus_tag="PKV23_07725"
                     /inference="COORDINATES: protein motif:HMM:NF024859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HQX85361.1"
                     /translation="MRVDHLVWYCADLAEGERFFAERMDRASAYGGVHPGEGTANRLL
                     SLSDATYLEILGRDPAQPETNLAPEVRSL"
BASE COUNT          516 a          629 c          690 g          379 t
ORIGIN      
        1 cgccatagtg gcgggcaccc ttggcatagg catgagtgac gccggacgga tccacatggc
       61 cgtccaccgg ttcgaaaagg gcgcccacat attttttggg atctatgagc gggttcaact
      121 cgcgcgcatg ttccagcgaa atgaaatccg aatccaggcc gagatagcgc gccttggccc
      181 gctcctgctt caggtagtcg taacgctccc tggtggaggc gagataaatc ccgccgggac
      241 ggtgaatgcc gcaggattgg cccgtgagct cttcaagctc cttgtaaaga ttgatggtgt
      301 agccctgcag ccgggcgatg ttggaatcgg aattgatcgt gtggatgatg cccgccgcat
      361 gccaggacga cccggacgtc agctccttgc gctcaagcaa caccacgtcg ttcaagccca
      421 acttggccag atgataaaga atggagcagc ccactacgcc gccgccgata accacaactt
      481 tcgcatgact ttgcattcgg gaaaccttca aaaagaaacg aatgtgtcag gtgaaaaggt
      541 tggtgtcaaa gctcacttct cctcatgctg aggggcatgg cgcagccgtg cctcgaagca
      601 ccagtttccg catccgccag cccctcgggg cctgctccgc agggcgcttc agggtgagga
      661 tggctggccc ttcatccgct ccgtgcccgg aaggaagatg gtgagaagcc cgatggccgg
      721 caataccgaa caaacctgga aaacaaaggt gatgctcgtg tgatcggcca ggtacccgag
      781 gggcacagcg ctcaggcccg cgatgccaaa ggcggtgccg aaaaacaggc cggagatcat
      841 ccccacgcgt cccggcaaca gttcctgtgc ataaaccacc atggcggaaa aggctgaggc
      901 gaggatgagg ccaatgggaa cggtgagcac aaccgtccag aacaggttgg caaagggcag
      961 cgccagtgtg aagggcaggg cgccgaggat ggaaaaccag attaccgcct tgcgcccgat
     1021 gcggtcgcca atgggacccc cggcaaatgt gccgatggca aaggcgccca gataaacgaa
     1081 gagataaatc tgcgcatcct tcaccggaac ctggaaatgg tcgatcagat agaaggtgta
     1141 gaaactgctg aagctcgagg tgtagacgaa tttcgagaac atcagcgcca gcaggatcag
     1201 aagcgacagg ctcaccgccc gcgaccccag attatggccg atggccgata cgggccggct
     1261 ggtgtgtgtg gggcggagat gctggctgta ccagcgtccg actccgctca gaatgccgac
     1321 cgccgcaagc gcaatcaccc cgaaccagac aagactggcc tgtccgcgcg gcaggatgat
     1381 ccaggcggcg agcagcggcc cgagcgatga acccacattg ccccccacct ggaacaggct
     1441 ttgcgccatg ccgaagcgtc cgcccgaagc gatgcgcgcc acgcgcgagg cctccgggtg
     1501 gaagatcgcc gagccgatgc ccactgctgc ggcggcaact agaatagccg cataggaggt
     1561 cgcaaacgcc aacagcagta cgcccaaaag tgtgaaagcc ggcgcaagag gcagggaata
     1621 gggcagcgga cggcggtcag tataaagccc gatcaggggt tgcaggatgg aggctgtgac
     1681 atggaacacc atcgtgatga tgccgatctg ggcaaaggtg agtgcatagg aatcccgcag
     1741 gatgggatag atggccagca tcagggcctg caaggtatcg ttgatcagat ggcacacggt
     1801 aacggaaatc agcaccgcaa atacggtgtt gctggcaatg cggttttcga ctgtggccac
     1861 gctggaattc ctgttcgtca ggccgcttta gcaagcccgg actgccgctg ccacccggcc
     1921 cggcgcaagg ctgatatgag cggagcacgt tgtaggttaa ctggacaccg gtcaatcagc
     1981 atgtaatttt tagtgcatgc gtgttgatca tcttgtctgg tattgcgcag acctggcgga
     2041 aggcgaacgc ttcttcgctg agagaatgga tcgcgcatcg gcctatggcg gggtgcatcc
     2101 cggagaagga accgccaacc ggctgctttc gctttccgat gcaacctatc tcgaaatcct
     2161 tggccgtgat ccggctcagc ctgagacgaa tctcgccccg gaagtccgga gtct
//
LOCUS       DAORVN010000366         2208 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_51173_length_2208_cov_2.777520, whole genome shotgun sequence.
ACCESSION   DAORVN010000366 DAORVN010000000
VERSION     DAORVN010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2208
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51173_length_2208_cov_2.777520"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..476
                     /locus_tag="PKV23_07730"
     CDS             <1..476
                     /locus_tag="PKV23_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019856973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HQX85362.1"
                     /translation="GNGTQEIFWSDPGLFYGSTHQMPLYPGSGAANETGVGNIFNAPL
                     RAGDGSVQFRDAMRSIILPALDDFAPDLVLISAGFDAHHADPLGSLQLSEEDFAWATL
                     ELMGAADAHSQGRVVSVLEGGYDLQGLAASVAIHVQALMRGTDALDHDDDEDDET"
     gene            473..718
                     /locus_tag="PKV23_07735"
     CDS             473..718
                     /locus_tag="PKV23_07735"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013169192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HQX85363.1"
                     /translation="MTKFPEIAALPFEKALAQLEEIVSKLESGKVDLEQSIAIYEKGQ
                     ALKEHCEKQLKDAEARIEKITLKPDGKPSGTAPLDVE"
     gene            715..1449
                     /locus_tag="PKV23_07740"
     CDS             715..1449
                     /locus_tag="PKV23_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006330594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HQX85364.1"
                     /translation="MTRHRLDELLVQRGLYSSRSRARDAIARGTVTVDGIVAAKPAQN
                     TGLESRIEILDDARNYVSRSALKLKSGLSFFNITAKGLNCLDTGASTGGFTQVLLEQG
                     AAHVTAIDVGHGQFDASLHSNPNVTSIEGLNARDLTAGHLKHKIDLIVCDVSFISLKL
                     ALPAALALAEAGTRLLALTKPQFEAGRDAIGRGGIVSDPQVHERVCKDVCAFLTAQNW
                     KVLGLTPASIEGGDGNREFLIAAVKA"
     gene            complement(1446..>2208)
                     /gene="aroC"
                     /locus_tag="PKV23_07745"
     CDS             complement(1446..>2208)
                     /gene="aroC"
                     /locus_tag="PKV23_07745"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011665964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HQX85365.1"
                     /translation="RPGHADLTYQAKYGLRDYRGGGRSSARETAARVAAGAVARKVIP
                     GVTIRGALVQMGTHKIDYKNWDWAEVHNNPFFSPDAKAAAQWEVYLDGVRKAGSSCGA
                     VVEVTASGVPAGWGAPVYGKLDQDLAAAMMSINAVKGVEIGAGFAAAAFSGEQNADEI
                     RMRHGKPEFLSNHAGGILGGISSGQDIVVRMAVKPTSSILTSRQTITAQGTNTDISTK
                     GRHDPCVGIRAVPVAEAMMALVLADHFLRHRAQIG"
BASE COUNT          432 a          746 c          628 g          402 t
ORIGIN      
        1 acggcaatgg cacccaggaa atcttctggt ccgatcccgg cctgttctat ggctctactc
       61 accagatgcc gctttacccc ggttccggtg cggccaatga aaccggcgtc ggcaacatct
      121 tcaatgcgcc gctcagggcg ggcgacggca gcgtgcagtt ccgcgatgcc atgcgcagca
      181 tcatcctgcc ggccctcgat gatttcgcac ccgatctggt gctcatctcg gcgggcttcg
      241 atgcccacca cgccgatccc ctgggcagcc tgcaattgag cgaagaggat ttcgcctggg
      301 cgacgctgga actcatgggc gcggcggatg cccattcgca aggccgcgtt gtgtccgttc
      361 tggaaggcgg ctatgacttg caggggctgg cggcatccgt tgccatccat gtgcaggccc
      421 tcatgcgggg caccgatgcg ctggaccacg acgatgacga ggatgatgag acatgacgaa
      481 atttccggaa atcgccgcac tgcccttcga aaaggctttg gcccagcttg aggaaatcgt
      541 ttcgaagctc gaatccggca aggtcgatct cgagcagtcc atcgccatct atgaaaaagg
      601 ccaagccctg aaggaacatt gcgaaaagca gttgaaggac gccgaagccc gcatcgagaa
      661 aatcaccctg aagcccgatg gcaagccatc cggaacggcg cctttggacg tggagtgaca
      721 cgccaccgcc ttgatgaact cctggttcag cgcgggcttt attcctcccg ttcccgtgca
      781 agggatgcca tcgcccgcgg caccgtgaca gtcgatggca tcgtagccgc caagcccgcg
      841 cagaacaccg gcctcgaaag ccgcattgaa attctcgatg atgcgcggaa ctatgtctcg
      901 cgttctgcgc tcaagctcaa gagtggactc agtttcttca atatcaccgc caagggcttg
      961 aattgcctgg atactggcgc atcgacagga ggcttcaccc aggtgcttct ggagcagggg
     1021 gcagcccatg tcaccgccat cgatgtgggc cacggccagt tcgacgcgtc acttcactca
     1081 aatccgaacg taacctcaat cgagggcctc aacgcccgcg atctcaccgc cggtcacctc
     1141 aagcacaaga tcgatctcat cgtctgcgat gtcagtttca tctccctgaa actggccttg
     1201 cccgccgcac tcgccctcgc cgaagccgga acccgacttc tggccctgac caaaccccag
     1261 ttcgaagccg gccgcgacgc cataggccgc ggcggcatcg tcagcgaccc gcaagtccat
     1321 gaacgcgtct gcaaggatgt atgcgcattc ctgaccgccc aaaactggaa agtcctcggc
     1381 ctcacccccg cctcaatcga aggcggcgac ggcaacaggg aattcctgat cgcggcggtg
     1441 aaggcctaac caatctgcgc ccggtgccgc aggaagtgat ccgccagcac cagcgccatc
     1501 atggcttcag caacgggcac ggcgcggatg ccgacgcagg ggtcgtgccg ccccttggtg
     1561 gagatatccg tgttcgtgcc ctgcgccgtg attgtttgcc gcgaggtgag aatggaggag
     1621 gtgggtttca ccgccatccg caccacgatg tcctggccgc tcgaaatgcc gcccaatatg
     1681 ccacccgcgt ggtttgacag aaactccggc ttgccgtggc gcatgcggat ttcatcggcg
     1741 ttctgctcgc cggaaaatgc cgccgccgca aagcctgcgc cgatttccac gcccttcacc
     1801 gcattgatgc tcatcatcgc cgccgccaga tcctggtcga gcttgccata aaccggcgca
     1861 ccccagcctg caggcacgcc cgaagccgtc acctcaacaa ccgcgccgca ggaagacccc
     1921 gccttgcgga ccccgtcgag atatacttcc cattgcgccg ccgcttttgc atccggcgag
     1981 aagaagggat tgttgtgaac ctcggcccag tcccagtttt tgtaatcgat cttgtgcgtg
     2041 cccatctgca ccagcgcgcc gcgaatggtg acgccaggaa tcactttccg cgccaccgca
     2101 ccggccgcca cccgcgccgc cgtttcccgc gccgaagagc gcccgccgcc gcgatagtcg
     2161 cgcaagccat atttcgcctg gtaggtcagg tccgcatggc ccgggcgg
//
LOCUS       DAORVN010000367         2203 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_51390_length_2203_cov_2.956238, whole genome shotgun sequence.
ACCESSION   DAORVN010000367 DAORVN010000000
VERSION     DAORVN010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2203
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51390_length_2203_cov_2.956238"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..575
                     /locus_tag="PKV23_07750"
     CDS             <1..575
                     /locus_tag="PKV23_07750"
                     /inference="COORDINATES: protein motif:HMM:NF013237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HQX85366.1"
                     /translation="KYIGGHGDAMGGAVLGRKTDLDALNLEATVHFGGVLSPFNAWLI
                     MRGAATLPLRMKAHQDAAMTLATRLEGHPAIKRVFYPGLASHPQHGLASTQMKNFSGM
                     MTFQTHEAGMAIARRMVKQLEIIHYAVSLGHLRSLIYWIGTDDIEQSTFRHGPEAASR
                     FRDYMGDGVFRLSVGIEDGDDLWADLVRCF"
     gene            complement(562..1371)
                     /locus_tag="PKV23_07755"
     CDS             complement(562..1371)
                     /locus_tag="PKV23_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018181807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylcholine/phosphatidylserine synthase"
                     /protein_id="HQX85367.1"
                     /translation="MAGDPEIDLPKKPANGRRFKQVPVRFLLPNLITLLALCSGITSI
                     RLGIEGRYELAVGAVIISIVLDAVDGRLARFLKGTSRFGAELDSLADFVNFGVAPAIL
                     IYLWSLNDMRTMGWVAVLILAVCCALRLARFNVALDDVDKPAWSASFFSGAPAPAGAG
                     LAMLPMYIGFLGIVDDGHTYSEFIAPYVVAVALLMVSRVPTYSGKTMRPRVSRDLVLP
                     ILGGGVLAIVCLIAFPWETLTLMAFAYLALIPFSIRAYRRYKAGDAQAKST"
     gene            complement(1374..2078)
                     /locus_tag="PKV23_07760"
     CDS             complement(1374..2078)
                     /locus_tag="PKV23_07760"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013503187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HQX85368.1"
                     /translation="MSIVSSITSAFVPIHREGYRFIAIFAAVTLVLFWLDWDALAWLG
                     VLATAWCAYFFRDPERVTPQRAGLVISPADGRISAIEKVIPPPELDLEREPTTRISVF
                     MNVFNVHVNRSPVDAKVARITYIPGVFLNADLDKASEDNERQAFTLELADGGKLGVVQ
                     IAGLVARRIVKFVGEGERLAAGQRLGLIRFGSRVDVYLPKGTNPLVAVGQSAIAGETV
                     LADLTSAEAERMARKS"
BASE COUNT          464 a          645 c          708 g          386 t
ORIGIN      
        1 caaaatatat cggcgggcat ggcgatgcca tgggcggcgc ggtgcttggc cgcaagacgg
       61 atctcgatgc cctcaacctt gaagcaacag tgcatttcgg cggcgtgctt tcgccattca
      121 atgcctggct gatcatgcgc ggggcggcga cgctgccgct gcgcatgaag gcgcatcagg
      181 acgcggccat gacgcttgcc acgcgccttg aagggcatcc ggcgatcaag cgggtgttct
      241 atccggggct tgcttcccat ccgcagcacg ggcttgccag cacgcagatg aagaatttct
      301 ccgggatgat gacgtttcaa acccatgaag ccggcatggc gattgcccgg cgcatggtga
      361 agcaactgga gatcatccac tatgcggtgt cgctgggcca tctgcgcagc ctgatctact
      421 ggatcggcac ggatgatatc gagcagtcca ccttccgcca tggcccggaa gcagcttcgc
      481 ggttccgcga ctacatgggc gacggggtgt tccgcctgtc agtcggcatc gaagatggcg
      541 atgatctctg ggccgacctg gtcaggtgct tttagcctgc gcgtccccag ccttgtagcg
      601 gcgataggcc cggatgctga aaggaatgag ggcgagatag gcaaaagcca tcagcgtgag
      661 cgtctcccag gggaaggcga tgaggcagac gatggcgagg actccgccac caagaatggg
      721 tagcaccaga tcgcgcgaca cgcgcgggcg catggttttc ccggaatagg tcggcacacg
      781 gctgaccatc aaaagtgcta ccgccacaac atagggcgcg atgaactccg aataggtgtg
      841 gccatcatca acgatgccca gaaagccaat atacatgggc agcatggcaa gcccggctcc
      901 agcgggtgcg ggagcgccgc tgaagaagct tgccgaccat gcgggtttgt ccacatcatc
      961 gagggccaca ttgaaacggg ccagcctgag ggcgcaacag acggccagga tcaggaccgc
     1021 gacccagccc atggtgcgca tgtcattgag cgaccagaga tagatcagga tggcgggagc
     1081 cacgccgaag ttcacaaaat ccgccagtga atcgagttcc gcgccaaagc gcgaggtgcc
     1141 tttcagaaag cgcgcgagcc tgccatcgac cgcgtcgagc acgatggaaa tgatgaccgc
     1201 gcccaccgcc agttcatagc ggccctcgat cccgagccgg atggaggtaa tgccgctgca
     1261 cagggccagc agggtgatga gattgggcag gaggaagcgc accggaacct gcttgaagcg
     1321 acggccattg gccggtttct ttggcaggtc aatttcagga tcgccggcca cgctcagctt
     1381 ttccgcgcca tgcgttcggc ctcggccgag gtaagatcgg ccagcacggt ttcgccggca
     1441 atggcgctct ggccaacggc caccagggga ttggttccct ttggcagata gacgtccacg
     1501 cggctgccga aacggatcag gccgagcctt tgcccggcgg caagacgttc gccttcgcct
     1561 acaaacttca caatccggcg ggccaccagc ccggcgatct ggacaacgcc gagcttgccg
     1621 ccatctgcca gctcgagggt aaaggcctgg cgctcgttgt cttcgcttgc cttgtcgaga
     1681 tcggcgttca ggaagacgcc cgggatgtaa gtgatcctgg caaccttcgc gtcgacgggc
     1741 gaacggttca catgcacatt gaagacgttc atgaacacgg atatgcgggt cgtgggttcg
     1801 cgctcgagat caagttccgg cggcgggatg actttttcga tggccgagat gcggccgtcg
     1861 gcaggcgaaa tcaccagccc tgcccgctgc ggcgtcacgc gttccgggtc gcggaagaaa
     1921 taggcgcacc aggcggtggc caagacgcca agccaggcca gcgcgtccca gtccagccag
     1981 aacagcacca gcgtaacagc ggcaaaaatc gcgatgaaac gataaccttc gcggtggatg
     2041 gggacaaatg ccgaggtgat gctgctgaca atggacatgg ggctcatcta gcgcggattt
     2101 ggcgttttgc aaagcaaaca cggagccatt acttggtaaa ggtctcttca ggcggcacgg
     2161 gcccagcggg ctgcagttct ccgggtttat taagcttatc ggt
//
LOCUS       DAORVN010000368         2200 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_51490_length_2200_cov_3.483450, whole genome shotgun sequence.
ACCESSION   DAORVN010000368 DAORVN010000000
VERSION     DAORVN010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2200
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51490_length_2200_cov_3.483450"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2200)
                     /locus_tag="PKV23_07765"
     CDS             complement(<1..>2200)
                     /locus_tag="PKV23_07765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85369.1"
                     /translation="EGQFRPVNYKSKVTGSFEKISLSEIEIRPAGVADQGMMLSGNAN
                     FVLGKQIKATADFSAPRVDFDALAGAGARRLLRDGDGLSLVNGLLAGLPEAIDLRSSI
                     KVAALKTGGEVLDNVLLDISANRNAMRIHELSASLPGRSRSRFEGVFFPGTQYAELAG
                     TLAVKSPDARQLSLWLWPDSRSAIARTWTGLRGNLEGKADVRLTASKLDLQNIEYVLD
                     GETGKAGLAVLVNGERPIIDLRIDMETADIDSYIPNGLAALSSDGSASWSSIVGNFVE
                     EQVKRDLHLTFQAGSLRLNGVEASDVAVDMETTVKGFDLKTIEAGSVGGAKLSVAGNV
                     LSTLEGPDGDIGISLMADDPRELLRLIGLLPRDANPRWSAVLGATSLKIDLQAKPSAA
                     APTTHFGIIGKVGDLDITSNGSFTMATGLAGMGLKGTTEIKSPSSATLFNLFGGAAEA
                     TDTIPALAVLTADGALQDGFQIDLRSDLYRSTVKFNGRVTPDPGRLVLDGNLTMQSAQ
                     AQDLLAALRVPGLAASGGDLSFATKISTAGTVQSFDEIEARLSGFSLTGSAALKDQTE
                     FTGDFDVGDISLMNTLAPVFLPWNGSAEPLEETFAKTLPFGLTGVVWVRPKSLEIYPG
                     FAVGNAQVGITATADVTEFVVSAKTVEGDDIVVEIASRQAVEGRKISGQVAVPFDLFQ
                     HLKRNDGRAVAAGQASVEAKFEGAGRSPGGVLAMLNGKGTFALRDGRLLN"
BASE COUNT          419 a          716 c          639 g          426 t
ORIGIN      
        1 atattcagaa gccggccatc ccgcaacgca aaagtgccct tgccattcaa catcgccagg
       61 acgccgccag gactccttcc cgcgccttca aactttgcct cgacactggc ctgcccggcg
      121 gcaacggccc ttccgtcatt gcgcttgaga tgctggaaga gatcgaatgg aacagccacc
      181 tgcccgctga tcttgcgccc ttcaaccgcc tggcgcgagg cgatttcgac gacaatgtca
      241 tcgccttcaa ctgttttggc ggaaaccaca aactcggtca catctgccgt ggcggtaatg
      301 cccacctgtg cattgcccac cgcaaatccg ggatagatct cgagtgactt tggccgcacc
      361 cacaccaccc cggtgaggcc gaaaggcagg gtcttggcaa aggtttcttc cagcggctcg
      421 gcgctgccat tccatggcag gaacaccggc gccagagtat tcatcagcga aatgtcgccc
      481 acatcgaaat cacccgtgaa ctccgtctgg tctttcaagg cggcgctgcc ggtcaggctg
      541 aaacccgaga gcctggcctc aatctcgtca aaggactgaa ccgtcccggc ggtgctgatt
      601 ttggtggcaa acgacaggtc tccacccgat gcggcgagcc ccggaacgcg cagcgcggcc
      661 agcagatcct gggcctgggc gctctgcatc gtgaggttgc cgtccaggac aagtctgccg
      721 ggatcgggag tcaccctgcc attgaacttc accgtcgaac gatacaggtc gcttcgcagg
      781 tcgatctgga agccgtcttg cagcgcgccg tcggcggtca ggaccgccag ggcaggaatt
      841 gtatccgttg cttcggcagc accgccaaac aggttgaaca gggtggcgct cgacgggctt
      901 ttgatctccg tggtgccctt gagccccatg ccggccaggc cggtggccat ggtaaagctt
      961 ccgttggaag tgatgtcgag atcacccacc ttgccgatga tcccgaaatg ggttgtgggg
     1021 gccgcggccg atggctttgc ctgcaggtcg attttcaggc tcgtcgcgcc gagaaccgcc
     1081 gaccaccggg ggttcgcgtc acgcggcagc aacccgatga gccgcaggag ttcgcgcggg
     1141 tcatccgcca tgagcgagat gccgatgtcg ccatcggggc cttccagcgt gctcagcaca
     1201 ttgccggcca cgcttagttt cgccccgccg accgagcctg cttcgatggt cttgagatca
     1261 aagcccttca cggtggtttc catgtccacc gcgacatctg aagcttcaac cccgttgagc
     1321 ctgaggcttc ccgcctggaa ggtgagatgc aagtcccgct tcacctgttc ctcgacgaaa
     1381 ttccccacaa tgctggacca gcttgcgctg ccgtcggaag aaagtgcggc gaggccattg
     1441 ggtatgtagc tgtcgatgtc ggctgtctcc atgtcgatgc gcaaatcgat gatggggcgc
     1501 tcgccattga ccagaaccgc aaggcccgcc ttgcccgttt cgccatcaag cacatactcg
     1561 atgttttgca ggtcgagctt cgaggccgtg agcctcacat ccgccttgcc ttccaggttt
     1621 ccacgaagcc cagtccaggt ccgggcaatt gcgctcctgc tgtcgggcca cagccacagc
     1681 gagagctgcc gcgcatccgg tgattttacc gccagcgtgc cggccagttc cgcatattgc
     1741 gtgcccggga aaaatacccc ttcaaagcgc gagcgcgagc gcccgggaag cgaggcggaa
     1801 agctcgtgga tgcgcatggc attgcggttt gccgagatat ccagcaagac attgtcgagg
     1861 acttcgcccc cggtcttgag cgccgcaacc ttgattgagg agcgcagatc aatggcctca
     1921 ggcaggccag ccaatagtcc gttcaccagc gaaaggccgt cgccatcgcg cagcaggcgg
     1981 cgcgccccgg cgcccgccag ggcatcgaaa tccacccgcg gcgctgaaaa atccgccgtc
     2041 gccttgatct gcttgccaag gacgaagttt gcattgcccg aaagcatcat gccctggtct
     2101 gcaacgcccg ccggcctgat ctcgatctcg ctcagcgaaa tcttttcaaa gctgccggtg
     2161 acctttgatt tgtaattgac cggacggaac tgcccttccg
//
LOCUS       DAORVN010000369         2197 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_51618_length_2197_cov_3.142390, whole genome shotgun sequence.
ACCESSION   DAORVN010000369 DAORVN010000000
VERSION     DAORVN010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2197
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51618_length_2197_cov_3.142390"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1242
                     /gene="cimA"
                     /locus_tag="PKV23_07770"
     CDS             <1..1242
                     /gene="cimA"
                     /locus_tag="PKV23_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018699926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HQX85370.1"
                     /translation="VRASVRLLTTAQSVEAAVFAGKEVLVDCEHFFDGFKANPEYALA
                     CARAAHDSGARWIVLCDTNGGTMPAEVSDIVRAVTKWVPGTHLGIHAHNDTDQAVANS
                     LAAIDAGCRHVQGTLNGIGERCGNANLVSIIATLGLKKSYRDRFAIAVDDERLQNLTH
                     VARAFDELLNRAPNAQAPYVGKSAFATKAGIHASAIVKEPETYEHVKPESVGNRRRML
                     VSDQAGKSNLVSELNRVGLNVSKDDKRLDTLLREVKEREAVGYAYDAADASFELLARR
                     TLGSVPHYFDVLSFRVIVEQRDGEMISEAVVKVKVDDETFINAGEGNGPVNALDVALR
                     KDLGKFQPYINDLELVDYKVRILNGGTGATTRVLIESRDGKGDRWFTVGVSPNIVEAS
                     FQALSDSIIYKLLREGVTGKS"
     gene            1317..>2197
                     /gene="rarD"
                     /locus_tag="PKV23_07775"
     CDS             1317..>2197
                     /gene="rarD"
                     /locus_tag="PKV23_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018687468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HQX85371.1"
                     /translation="MAAIEQTSKAGLTAAEKGVLYAVLAHLTWGAMAVYFGFMRHISP
                     IEIAVHRGLWAIPCAALIIWWLGQYKDVWQAVASKRNLAILTLTGSIIVFNWGFYVWS
                     IEMGRTLEASLGYYINPLMNVVMGYLFLGERFNRLQLLALLLALIAVLIQTFAAGVFP
                     WLGLMLATSFCIYGFLRKTIAVGPTQGFFIEVCIIAIPLLLTQVWLVNNGEAHFGGTL
                     PDTLLLMGLGLMTTSALVFFALSIKLLRYSTAGLLQYISPTLVFLTAVFVFGEPMDSW
                     KLLSFVVLWIGLAIYSWS"
BASE COUNT          394 a          719 c          615 g          469 t
ORIGIN      
        1 gtccgtgcgt ccgttcgcct gttgacaacg gctcagtccg tcgaggccgc ggttttcgcc
       61 ggcaaggaag tgctggtcga ctgcgagcat ttcttcgacg gcttcaaggc caacccggaa
      121 tacgccctcg cctgcgcccg ggcggcccat gattccggcg cgcgctggat cgtgctctgc
      181 gacaccaatg gcggcaccat gcccgctgaa gtttccgaca tcgtccgtgc cgtgacgaaa
      241 tgggtgccgg gaacccacct cggcattcac gcccataatg acaccgatca ggccgtggcc
      301 aattccctgg ccgcaatcga tgctggctgc cgccatgtcc agggcaccct gaacggcatc
      361 ggcgaacgct gcggcaacgc caacctcgtc tcgatcatcg ccaccctcgg cctgaagaaa
      421 tcctaccgcg accgctttgc catcgcagtt gatgacgagc gcctgcagaa ccttacccat
      481 gtggcccgcg ccttcgacga actgctgaac cgcgccccca acgcccaggc tccctatgtg
      541 ggcaagagtg cctttgccac caaggccggc atccacgcct ctgccatcgt caaggaaccg
      601 gaaacctatg agcatgtgaa gccggaatcc gtgggcaacc gccgccgcat gctggtctcc
      661 gaccaggccg ggaaatccaa tctggtgtcc gagctgaacc gcgtcggcct gaatgtctcc
      721 aaggacgaca agcgcctcga cacgctgctc cgcgaggtga aggagcgcga ggccgtgggc
      781 tatgcctatg acgccgccga tgcctccttc gagctcctcg ccaggcgcac ccttggcagt
      841 gtgccccatt actttgacgt gctctccttc cgcgtcatcg tcgaacagcg cgacggcgaa
      901 atgatctctg aagccgtggt caaggtgaag gtcgatgacg agacctttat caacgcgggc
      961 gagggcaatg gcccggtgaa cgccctcgac gtggcgctgc gcaaggacct cggcaagttc
     1021 cagccctata tcaacgatct cgaactggtc gactacaagg tgcgtatcct caatggcgga
     1081 accggagcca caacccgggt tctgatcgaa agccgcgacg gcaagggcga ccgctggttc
     1141 accgtgggcg tttcccccaa tatcgtcgag gcctccttcc aagccctctc ggattccatc
     1201 atctacaagc tgctgcgcga aggcgtgacc ggcaagagct gacatgcggg gtgctcatgc
     1261 gacccatgtt cgttctctga ttgccgcccc tgagtggcgc agttaaaccc ccgggcatgg
     1321 cagccatcga acaaacttca aaagccggcc tgacagcagc cgaaaaaggc gttctttacg
     1381 cggttctggc gcatctcacc tggggcgcca tggccgtcta tttcggcttt atgcggcaca
     1441 tctcaccgat cgaaattgcc gtgcatcgcg gcctctgggc cattccctgc gccgccctga
     1501 tcatctggtg gcttggtcaa tacaaggatg tctggcaggc cgtcgcctcg aagcgcaatc
     1561 tggccattct cacgctcacc ggctccatca tcgtgttcaa ctggggcttt tatgtctggt
     1621 ccatcgagat gggccgcact ctggaagcca gcctcggcta ttacatcaac ccgctcatga
     1681 atgtggtcat gggatacctg tttttgggcg aacgcttcaa ccgcctgcag ttgctggcgc
     1741 tcctccttgc gctgattgcg gtgctgatcc agacatttgc ggcaggcgtg tttccctggc
     1801 ttggcctgat gctggccaca agcttctgca tttacggttt cctgcgcaag accattgcgg
     1861 tgggtcccac ccagggcttc ttcattgaag tctgcatcat cgccatcccg ctcttgctca
     1921 cgcaggtctg gcttgtgaac aatggcgaag cccattttgg cggcacactg cccgacacct
     1981 tgctgctgat gggcctgggg ttaatgacga catcggcgct ggttttcttc gcgctctcta
     2041 taaaattgct gcgctattcg actgccgggc tgctgcaata catctcgccc accctggtgt
     2101 tcctcaccgc cgtgtttgtg ttcggtgaac ccatggacag ctggaagctc ctgtccttcg
     2161 tcgtcctgtg gattggcctt gcgatctatt cctggtc
//
LOCUS       DAORVN010000370         2197 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_51620_length_2197_cov_3.016807, whole genome shotgun sequence.
ACCESSION   DAORVN010000370 DAORVN010000000
VERSION     DAORVN010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2197
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51620_length_2197_cov_3.016807"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1312
                     /locus_tag="PKV23_07780"
     CDS             <1..1312
                     /locus_tag="PKV23_07780"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HQX85372.1"
                     /translation="VGQLFWGPVADRVGRRPVLMLGLLSILASPVAHAGPSVLFDAAT
                     GEVITHDRAGEPWYPASLTKLMTAYIVFKKLKAGTLRLDQKILVSPLAASQEPSKIGM
                     RPGSAISVDLALQTLLVYSANDMAYVLAEGANGTVFSFVQEMNATAKKLGLSATHFVN
                     PNGLFDPRQLTSARDIGVLAAVILAEFPEYSGYFSQQHVAIGKKKLLNRNSLIRSMPE
                     ADGMKTGFVCNSGFNLVASATRDGRKLIAVVLGAPNSGSRAEIARTLLAEGFPKGTLA
                     SRPRLAQISNSPLGAIVPADLTSTVCKKKPPVTAVRARELAGWGISFGSYDTLQKADM
                     ALRGRLISPAGMDAPGKAGVVRMPNKQGFAAMLWNIDQATSQALCSDYRSQNAVCEVM
                     TPAAFAQIAALSKEPEPKPKVQAPVAQGSDGQKPAKKKIKKTAN"
     gene            1323..>2197
                     /locus_tag="PKV23_07785"
     CDS             1323..>2197
                     /locus_tag="PKV23_07785"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HQX85373.1"
                     /translation="MISRIRLGVGCALAAASIYGLVPNFVRGAFENGIPPVEATFFRT
                     SVIAVAFALIAVVQGESFRVPRAAIAAFAGQAVATLVISVSYLASVQFIPIGLAVIIF
                     FTFPVLIMLASPMVEGKFPGFWRIVIAVVAFGGLVIAIGPSFGELDIRGIGLATLASL
                     GGVVQFFTGRSISRYLTPAVFGSLVHIVIWPATLLVALYVSGGTLQMMPGGAGTATGF
                     YFLLGVAGIYVAAYMLQMSSLRFAPASTVAPFYNLEPIVTTAFAGLLFGEILTANQYA
                     GGGIVLAALVVSSRLG"
BASE COUNT          395 a          652 c          655 g          495 t
ORIGIN      
        1 cgtcggccaa ctgttctggg gcccggtggc tgatcgcgtg ggccggcggc cggttctgat
       61 gctggggctg cttagtatcc ttgcatcacc tgtggcccat gcggggcctt cagttttgtt
      121 tgatgccgcg acaggcgaag tcattacgca cgaccgggcg ggcgaaccct ggtatccagc
      181 ttcgctcacc aagctgatga ccgcctatat tgttttcaag aaactgaaag ccggcacctt
      241 gcgccttgac cagaagatcc tggtgtcgcc cctggccgct tcgcaggagc ccagcaagat
      301 cggcatgcgc ccgggcagcg ccatttcggt tgacctggca cttcagacgt tgctggtcta
      361 ttcggccaat gacatggcct atgtgcttgc cgaaggcgcc aatggcacgg ttttcagttt
      421 tgtccaggaa atgaatgcga ctgccaagaa gctgggactg agtgccactc atttcgtaaa
      481 tcccaacggc ctgtttgatc cacgccagct cacatcggca cgcgatatcg gtgttcttgc
      541 tgccgtcatc cttgcggagt ttcccgagta cagcggctat ttttcccagc agcatgtggc
      601 gatcggcaag aaaaaactgc tcaaccgcaa cagcctgatc cggagcatgc cggaagcgga
      661 tggcatgaaa accggattcg tctgcaattc tgggttcaat ctcgtggctt cggcgacgcg
      721 tgacggacgc aaactgattg ccgttgttct gggggccccc aattccggca gccgcgcgga
      781 aatcgcgcgg acactcctgg cggaaggatt tccaaagggg accctggcaa gccgcccgcg
      841 cctggcgcag atctccaaca gcccattggg cgccattgtt ccggcagatc tcaccagcac
      901 ggtatgcaag aagaagccgc cagtgactgc ggtccgcgcc agggaactcg ccggatgggg
      961 gatttccttc ggaagttacg acaccctgca aaaggccgac atggcgctgc gcggacggct
     1021 gatcagcccc gccggcatgg atgcgccggg caaggccggt gtcgtgcgca tgcccaataa
     1081 gcaaggcttt gcggccatgc tctggaatat tgatcaagcc accagccagg cgctgtgcag
     1141 tgactatcgt tcgcaaaacg cggtgtgtga agtcatgacg cccgcggcct ttgcccagat
     1201 tgcggcgctg tcaaaggagc cggaacccaa gcccaaggtg caggcgccgg tggcgcaagg
     1261 ctctgacggg cagaaaccag ccaaaaagaa aatcaagaaa accgccaact gagccctgaa
     1321 atatgatcag caggatcagg cttggagttg gttgcgccct ggcggcggcc agcatttatg
     1381 gccttgtccc caattttgtc cgtggtgcct ttgaaaatgg gattccgccg gttgaagcca
     1441 cgttcttccg aacttcggtg attgcggtcg cgtttgccct catcgccgtg gtgcagggag
     1501 agagcttcag agttccccgc gccgctattg ccgccttcgc ggggcaggcc gtcgccaccc
     1561 tggtgatctc ggtgtcctat ctggcgtcgg tccagttcat tcctatcggc cttgcggtca
     1621 tcatcttttt cactttcccg gttctcatca tgcttgcctc gcccatggtt gaagggaagt
     1681 ttcccggatt ttggcgaatc gtgatcgcag ttgtcgcgtt tggcggactg gtcatcgcca
     1741 tcggcccgag cttcggcgag cttgatattc gcggcatcgg gcttgcaaca cttgcctcac
     1801 tcggcggcgt tgtgcagttt ttcaccgggc gatcgatctc gcgctatctc acgcccgccg
     1861 tgttcggcag tctggtgcat atcgtcatat ggccggcaac cctgctggtc gcgctctatg
     1921 tgagcggggg aaccctgcag atgatgccgg gcggcgccgg cacggcgacg ggtttttatt
     1981 tcctcttggg agtggccggc atctatgtcg ctgcctatat gctgcaaatg tcttctctgc
     2041 gctttgcgcc agcatcaact gtcgcaccct tctataatct ggagccgatt gtcaccacgg
     2101 cttttgccgg gctgctgttt ggcgaaatcc tcacggccaa tcaatatgcc gggggcggca
     2161 ttgttcttgc cgctcttgtc gtatcaagcc gcctggg
//
LOCUS       DAORVN010000371         2190 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_51938_length_2190_cov_2.746136, whole genome shotgun sequence.
ACCESSION   DAORVN010000371 DAORVN010000000
VERSION     DAORVN010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51938_length_2190_cov_2.746136"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            450..1073
                     /locus_tag="PKV23_07790"
     CDS             450..1073
                     /locus_tag="PKV23_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008190473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HQX85374.1"
                     /translation="MPNPNAASPSSRLADEEDDAAPPSIKNSRLVDRHYFDSRIVMVT
                     GEINHTMAERVCVNLFALAQAGNDPITMIISSPGGHVESGDMIHDMIKFIKPRVRVLG
                     SGWVASAGALIYIAADKKDRYILPNTRFLLHAPSGGVGGKVTDIGIQAKEMMLMRERL
                     NKLFAEATGQPLKKIEKDTDRDFWLSGREAIDYGLATHMITSMSELK"
     gene            complement(1083..1850)
                     /locus_tag="PKV23_07795"
     CDS             complement(1083..1850)
                     /locus_tag="PKV23_07795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85375.1"
                     /translation="MNVSQRLAFSLLMCAGFALTAPIAAAQDGMRPVGQLTYTPEPAP
                     PQTGVFQLRPDDRMIRSMRIEARGGTVDIQSVRLVYRNGEQERYRIRERLRPGQSTGI
                     IRKQNRGPLRAVEVSYIPQGQVTLVLRASAGGGGGGEPPPVATWNELGCKTVGFLIDR
                     DRVNVSSEDFYRALRLRSTGLDIEMLDMNVRFANGQTDSYRVNTVIRSGERSRPIDLR
                     GERRRISSIEFIYRSLGISTQKTKLCVDGLQIERDDE"
     gene            complement(1892..>2190)
                     /locus_tag="PKV23_07800"
     CDS             complement(1892..>2190)
                     /locus_tag="PKV23_07800"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQX85376.1"
                     /translation="AALLELEEIPLGSYYVNIIAVYPQRRRSGAGAALMRDAEHLARV
                     QKFSRMSLCTFEDDPGLMGFYRGQGFEPQARRAIVPHPAIGKSGHFVLMTRALQ"
BASE COUNT          431 a          772 c          617 g          370 t
ORIGIN      
        1 taagacccgc gcgccgcaag gcagttaggc agtctgaaga agacagggcc gaagtgcgaa
       61 ccggaagccg aaaccgccgc aaaagcgtgg gcgcagtttg cgtcacccgc cggacgtcac
      121 gaccggcact tctgcgtagg ctgcgttcac gcaacccttc gcttctcgcc tgccccggaa
      181 acggcataac cgtttccacc aacggcgaag ccatgcccac ccttgccctt ctcccctcga
      241 gggagaaggt ggcgcggaac gcgccggatg agggggcctt cgcggccacc actcaattgc
      301 atcaaacaca cccctcaccc gtcactcact acgtgagtgc caccctctcc ctcaagggga
      361 gagggaaaaa cgcactaccc acgggatgac aatcactccg ccagccccta tatctcctcc
      421 gaatcccctc aaaaccagcg agatcaagaa tgcccaatcc gaatgccgcc tctccctcct
      481 cccgcctcgc cgatgaggag gacgatgccg ccccgccgag catcaagaat tcccgcctcg
      541 tcgacaggca ttatttcgac tcgcgcatcg tcatggtcac cggtgaaatc aaccacacca
      601 tggccgagcg cgtctgcgtc aatctctttg ccctggccca ggccggcaat gatcccatca
      661 ccatgatcat ctcgtccccc ggcggccatg tggaatccgg cgacatgatc catgacatga
      721 tcaagttcat caagccgcgt gtccgcgttt tgggctccgg ctgggtcgcc agcgccggcg
      781 ccttgatcta catcgccgcc gacaagaagg accgttacat tctgcccaac acccgcttcc
      841 tgcttcacgc gccctcaggc ggcgtcggcg gcaaggtcac cgacatcggc atccaggcca
      901 aggaaatgat gctgatgcgc gaacgcctca acaagctttt cgccgaggcc accggccagc
      961 ccctcaagaa aatcgaaaag gacaccgacc gcgatttctg gctctccggc cgcgaagcca
     1021 tcgactacgg cctcgccacc cacatgatca cctcgatgtc cgagctgaaa taggccgggt
     1081 gattattcgt cgtcgcgctc gatctgcagc ccgtcaacgc agagctttgt cttctgggtg
     1141 gaaatcccca gtgagcggta gatgaattcg atgctggaaa tgcggcggcg ttcgcccctg
     1201 agatcaatgg gccgcgagcg ctcgcccgag cggatcaccg tattgacccg gtaggagtcc
     1261 gtctggccat tggcgaagcg cacgttcatg tccagcatct cgatgtcgag gccggtggag
     1321 cgcaggcgca gcgcccggta gaaatcctcc gagctcacgt tcaccctgtc gcggtcgatg
     1381 aggaagccca cggtcttgca gcccagttcg ttccacgtgg caaccggcgg cggctcgccg
     1441 cccccgccgc cgccagcact tgcgcgcagc accagggtca cctggccctg cggaatgtag
     1501 cttacttcaa ccgcgcgcaa gggcccgcgg ttctgcttgc ggatgatccc ggtcgattgc
     1561 cccggccgca gccgctcgcg gatgcggtag cgctcctgct cgccattgcg gtaaaccagc
     1621 cgcacgctct ggatgtcaac cgtgccgccc cgcgcctcga tccgcatgct gcggatcatc
     1681 cggtcgtccg gccgcagctg gaacaccccg gtctgcggcg gcgccggctc cggcgtatag
     1741 gtcagctggc caaccggccg catcccgtcc tgggcggcgg caatgggtgc ggtcaaggca
     1801 aagccggcac acatcaacag gctgaaagca aggcgctggg aaacattcat ggagtaacct
     1861 catatgagtc gcttggggcc cggaagttac ctcactgaag cgcccgcgtc atcagcacaa
     1921 aatgcccgga tttgccgatt gccgggtgtg gaacaatcgc ccgccgcgcc tgcggttcaa
     1981 acccctgccc ccggtaaaat cccatgaggc ccgggtcatc ctcgaaggtg caaaggctca
     2041 tgcgggaaaa tttctgcacc cgcgccaggt gctcggcgtc gcgcatcagc gccgcccccg
     2101 ccccgctccg ccggcgctgc ggataaaccg caatgatgtt cacataatag ctgcccagcg
     2161 ggatttcctc gagctccaga agcgccgcga
//
LOCUS       DAORVN010000372         2188 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_52014_length_2188_cov_3.413033, whole genome shotgun sequence.
ACCESSION   DAORVN010000372 DAORVN010000000
VERSION     DAORVN010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2188)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52014_length_2188_cov_3.413033"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            3..743
                     /locus_tag="PKV23_07805"
     CDS             3..743
                     /locus_tag="PKV23_07805"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011089252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HQX85377.1"
                     /translation="MDRTGTGTKSVFGYQMRFDLGAGFPLVTTKRLHLRSIIYELLWM
                     LRGDTNIKYLHDNKVTIWDEWADVNGDLGPVYGQQWRSWPTRDGGVIDQIADTVERIK
                     KNPDSRRLLVSAWNPGDVDRMALPPCHCLFQFYVHNGRLSCQLYQRSADIFLGVPFNI
                     ASYALLTHMMAHVTGLKAGEFIHTLGDAHIYLNHLEQIDEQLSREPYAPPRLVMKREV
                     KAIDEFRYEDFEIVNYQAHPNISGSVAI"
     gene            749..1567
                     /locus_tag="PKV23_07810"
     CDS             749..1567
                     /locus_tag="PKV23_07810"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HQX85378.1"
                     /translation="MEKFGTDFQKYTSPLLYAPSNPAGLWLALALALLLVVANGVFQA
                     AAGTTILSVFFDPALADQKSMIKAYIIGIFPASLLTVVLAWQVAKIRGGRPAEVLALR
                     WPDLGWLGWATVIGGFMIAMYAAIFVLVAVFQVDLADYTPGPDGQSPTSGSAGLVKEA
                     MFDLANEPRLFWLAFPAVAIGAPLAEELVFRGQLFAALARSRAGYSGAVLITSALWSL
                     LHITEPWLAVGIIFMMGLVLGALLIRFGSLWVTIACHAIWNGLYSLVIFETLPQ"
     gene            1564..2043
                     /locus_tag="PKV23_07815"
     CDS             1564..2043
                     /locus_tag="PKV23_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016841067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQX85379.1"
                     /translation="MSLLIREAVRGDEQLVFAYICKLADYESLRHEVSASAADIGESL
                     FGPTPRAHCDLAFWQGKPAGFALWFYNYSTFRGRAGIYLEDFFVDEAMRGLGIGKALL
                     KHLAQRCVAEGLPRLQWWVLDWNTPSIEFYKSLGAVPQDEWTVFRVSGEALGKLAAQ"
BASE COUNT          372 a          641 c          690 g          485 t
ORIGIN      
        1 aaatggaccg taccggcacc ggtacgaagt ccgttttcgg ctaccagatg cgttttgacc
       61 tcggcgccgg ttttccgctg gtgacgacca agcggctgca tctgcgctcc atcatttatg
      121 agctgctgtg gatgctgcgc ggcgacacca acatcaaata cctgcacgac aacaaggtca
      181 cgatctggga tgagtgggcc gatgtgaatg gcgacctcgg tcccgtctat gggcagcagt
      241 ggcggtcgtg gccgacgcgt gacggtggcg tcatcgacca gatcgccgac acggttgagc
      301 gcatcaagaa aaatcccgac tcgcgccggc tgctggtgag cgcgtggaat cccggcgatg
      361 tggacaggat ggccctgccg ccctgccatt gcctgttcca gttctatgtg cacaacggca
      421 ggctctcatg ccagctttac cagcggtcgg cggatatttt cctcggcgtg ccgttcaaca
      481 tcgcgtccta cgccctgctc acccacatga tggcccatgt cacgggactt aaggcaggcg
      541 aattcatcca cacgctggga gacgcccata tctatctcaa tcatcttgag cagattgacg
      601 agcagctgtc gcgtgagccc tatgcccctc cgcgccttgt gatgaagcgc gaggtcaagg
      661 ccatcgatga attccgctat gaggactttg aaatcgtgaa ctatcaggca catccgaata
      721 tttccgggtc cgtggcaatc tagtcacgat ggaaaaattc ggtacggact tccagaaata
      781 tacttcgccc ctgctttatg cgcccagcaa tccggcgggg ctgtggctgg cgctcgcgct
      841 tgccctgctt ctggttgtgg ccaacggagt gttccaggcg gctgccggaa ccaccattct
      901 cagcgtgttt tttgacccgg ctctggcgga tcagaaatcc atgatcaagg cttacatcat
      961 cggcatattt ccggcctcgc tcctgaccgt ggtgctggcc tggcaggtgg ccaagattcg
     1021 cggcggccgc ccggcggagg ttctggccct gcgctggccc gacctgggct ggctgggatg
     1081 ggctacagtc atcggcggtt tcatgatcgc gatgtatgcg gcgatcttcg ttctcgttgc
     1141 ggtgtttcag gtcgatcttg cggactacac gccgggcccc gacgggcagt cgcccacgtc
     1201 cggttcagcc gggctggtga aagaggcgat gtttgacctg gccaatgagc cgcgcctgtt
     1261 ctggctggct tttccggcgg tcgccatcgg cgcgccgctg gccgaagaac tggtgttccg
     1321 cggccagttg tttgccgcac tggcgcgaag ccgggcgggc tattcgggtg ccgtgctcat
     1381 cacctcggcg ctgtggtcgc tgctgcacat caccgagccc tggcttgcgg tgggcatcat
     1441 cttcatgatg ggactggtcc tgggcgcgct gctgatccgc tttggcagcc tgtgggtgac
     1501 gattgcctgc cacgccatct ggaacggcct ctattccctg gtcatttttg agaccttgcc
     1561 gcaatgagcc tgctgatccg ggaagcggtt cggggcgatg agcaactcgt attcgcgtac
     1621 atctgcaagc tagcagatta tgaaagcctg cgccacgagg tgtcagccag cgccgctgac
     1681 atcggcgaaa gcctgttcgg gccaacgccg cgggcccatt gcgatctagc tttctggcaa
     1741 ggcaagcccg ctggctttgc cttgtggttc tacaattact ccacttttcg cgggcgggcc
     1801 gggatttatc tcgaggattt tttcgtcgat gaggcgatgc gcggccttgg catcggcaag
     1861 gcgctactga aacatttggc gcagcgctgc gtggcggaag gactgccgcg cctgcagtgg
     1921 tgggtgctgg actggaacac gccgtcgatt gaattctata agtccctggg agcggtgcca
     1981 caggatgagt ggacggtgtt tcgcgtttcc ggcgaagccc tgggcaagct ggcggcacaa
     2041 tgatcacggt gcgcccgatg cagcgcggcg aaggggccgc actcctggag atggttcgcg
     2101 ccctggcgga gtcgcacaat cttctgcata cggtgctggc aaccccggag atgtttgaag
     2161 cggcgatgtt ttcggaggcc ccgattgt
//
LOCUS       DAORVN010000373         2186 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_52114_length_2186_cov_3.009385, whole genome shotgun sequence.
ACCESSION   DAORVN010000373 DAORVN010000000
VERSION     DAORVN010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2186
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52114_length_2186_cov_3.009385"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1257)
                     /gene="fabF"
                     /locus_tag="PKV23_07820"
     CDS             complement(<1..1257)
                     /gene="fabF"
                     /locus_tag="PKV23_07820"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018390321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HQX85380.1"
                     /translation="MRRVVVTGLGIVSPLANGIDETWSRMLAGASGAGPITKFDASDL
                     PCRIAFEVKRGDGSNGTLNLDQWLDGKEQRRNDDFIQYALIAARQALTDSGYEIKTPE
                     QQYRTGVLVGSGIGGLPSIEETSILLHEKGPRRISPFFIPGALINLASGQISIAHGLK
                     GPNHAVVTACSSGTHAIGDAARLIALDDADVMFAGGTESCINRLGIAGFAACKALSTN
                     FNETPAKASRPYDKDRDGFVMGEGSGMLILEELEHAKARGAKIYAEIVGYGLSGDAYH
                     ITSPPEDGDGAFRCMKAALKRAGIAPTEIDYINAHGTSTPVGDEIELRAVERIMDGKV
                     ERLSMSSTKSSTGHLLGAAGAVEAIFSILAIRDNVCPPTLNLDNPSVATNIDLVPNRP
                     RKKEVNIVLSNSFGFGGTNASIVMRRF"
     gene            complement(1353..1589)
                     /locus_tag="PKV23_07825"
     CDS             complement(1353..1589)
                     /locus_tag="PKV23_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019170544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HQX85381.1"
                     /translation="MSDVAERVKKIVVEHLGVDGDKVKTEASFIDDLGADSLDTVELV
                     MAFEEEFAIEIPDDAAETIQTVGDAVKFIEKAAA"
     gene            complement(1718..>2186)
                     /gene="fabG"
                     /locus_tag="PKV23_07830"
     CDS             complement(1718..>2186)
                     /gene="fabG"
                     /locus_tag="PKV23_07830"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002716126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HQX85382.1"
                     /translation="TRDGLAMRMKDEDWAAVLDVNLTAGFRLCRAAMRPMMKKRWGRI
                     VNITSVVGVTGNPGQANYVASKAGIIGLSKSLAQELASRNVTVNCVAPGFIATPMTDV
                     LSDKQKESILARVPAGRLGSPDDIAAATLYLASDEAAYVTGQTLHVNGGMAMI"
BASE COUNT          416 a          625 c          689 g          456 t
ORIGIN      
        1 aaagcggcgc atgacgattg atgcattggt accaccaaaa ccaaaagaat tggacagaac
       61 tatgttgacc tctttcttgc gtgggcgatt gggaacgaga tcgatgttcg tcgcaaccga
      121 aggattgtcc aggttcaagg tcggcgggca gacattgtca cgaatcgcca gaattgaaaa
      181 tatggcttca acggccccgg cggcgccgag cagatggccg gtggaggact tggtggacga
      241 catggacagg cgctcaacct tgccatccat gatgcgctcg acggcgcgca gttcgatctc
      301 gtcgcccacc ggcgtggatg tgccatgggc attgatgtag tcgatttccg tcggggcaat
      361 gccggcccgc ttcagggcgg ctttcatgca gcggaaggcg ccatcaccgt cttcgggcgg
      421 cgaggtgatg tgataggcat cgccggacag gccatagccc acgatctcgg cgtatatttt
      481 cgcgccacgg gctttggcat gttcaagctc ctcaaggatg agcatgccgg agccctcgcc
      541 catgacaaag ccgtcacggt ccttgtcgta ggggcgcgac gccttggccg gcgtctcgtt
      601 gaagttggtg gacagggcct tgcaggcggc gaagccggca atgcccaggc ggttgatgca
      661 ggattcggtg ccgccggcga acatgacatc ggcatcatcc agcgcaatca ggcgcgccgc
      721 gtcgccgatg gcgtgggtgc cggaggaaca ggcggtgacg actgcgtggt ttggaccttt
      781 gaggccatgg gcgatggaaa tctggcccga ggccaggttg atgagggcgc cgggaatgaa
      841 aaacgggctg atgcggcgcg ggcctttttc atgcagcaat atggaggttt cctcgatgct
      901 gggaaggccg ccgatgccgg agcccaccag cacgccggta cggtattgct gttccggcgt
      961 cttgatttcg tagcctgaat cggtgagggc ctgcctggcg gcgatcaacg catactgaat
     1021 gaaatcatcg ttgcggcgct gctccttgcc gtcgagccac tggtccaggt tcagggtgcc
     1081 gttgctgcca tcgccgcgct tcacctcaaa ggcgatgcgg caaggcagat cgctggcgtc
     1141 aaacttggtg atgggaccgg cgcctgatgc gcccgccagc atgcgggacc aggtttcgtc
     1201 tattccgttg gcaaggggac tgacgatccc caatccggtc actacgacac gacgcatgcg
     1261 ctctccctta aatgaaaacg cgggggccag ggcgtttcac atccctgccc ccgcgtggcg
     1321 attgattatt gccccgctca gggggcgaaa ccttaagcag cggccttctc aataaacttc
     1381 actgcatcgc caacggtctg aattgtttcg gcagcgtcgt cgggaatttc aatggcgaat
     1441 tcctcttcga aagccatgac gagttcaacc gtgtcgaggc tgtcggcgcc gaggtcgtca
     1501 ataaagcttg cctcggtttt cactttgtcg ccgtccacac cgaggtgctc gacaacgatc
     1561 ttctttaccc gctcagcaac gtcactcatg ttcaatcgtc cttgtttgct tggaatcata
     1621 cttaggttgg acacccggtt tttggtggtt cccccttgtg cctacccgct aactaccaca
     1681 gaatccaatt gcggcaaaag ggggataatt tattcaatca aatcatcgcc atgccgccat
     1741 tcacatggag ggtctggccg gtcacatagg cggcttcgtc cgacgcgagg tatagggtgg
     1801 cggcggcgat gtcatcgggt gagcccagcc tgccggcggg aaccctggcg aggatcgatt
     1861 ccttctgctt gtcgctcaga acatcggtca tgggcgttgc gatgaagccg ggggcgacgc
     1921 aattcaccgt cacattgcgc gaagccagtt cctgggccag ggacttggac aggccgatga
     1981 tgccggcctt ggaggccacg taatttgcct ggccgggatt gccggtcacg cccaccacgg
     2041 acgttatgtt cacgatgcgg ccccagcgct ttttcatcat ggggcgcatg gcggcgcggc
     2101 acaggcggaa gccggcggtc aggttcacgt caagcacggc ggcccagtcc tcatccttca
     2161 tgcgcatggc gaggccatcg cgggtg
//
LOCUS       DAORVN010000374         2176 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_52536_length_2176_cov_3.413484, whole genome shotgun sequence.
ACCESSION   DAORVN010000374 DAORVN010000000
VERSION     DAORVN010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2176
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52536_length_2176_cov_3.413484"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            50..1129
                     /locus_tag="PKV23_07835"
     CDS             50..1129
                     /locus_tag="PKV23_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HQX85383.1"
                     /translation="MAGAALAMPYISRAHAQGEALNVFNWTDYIGETTIEDFQSATGI
                     LVAYDLYDSTEAMEAKMLAGSSGFDVVVQAGSTLERFTEAKVYQPLDRSKLTNWGNLD
                     PVSLKTVEGWDPGNVYGVPYMWGTVGLTFNVDMVKERIPDADFTTLDILFKPENAAKL
                     ADCGISILESPGDVIPMALAYLGLDPNTTNPADFDAVVKLFAPIRQHIRAFDASNYLN
                     AIPNKELCMINNWSGDYATANARAAEAGIQLNLSYHVPKTGSPAWFDIWCIPADAKHV
                     DNAHKFINYMMEPEVIAKCTNYTNYANVNLAADKFVDPAILADPAVYPDAELKKRLWT
                     QKSMSSDLERARTRAWSTIKTGSPS"
     gene            1180..2106
                     /locus_tag="PKV23_07840"
                     /pseudo
     CDS             1180..2106
                     /locus_tag="PKV23_07840"
                     /inference="COORDINATES: protein motif:HMM:TIGR01187.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            2086..>2176
                     /locus_tag="PKV23_07845"
     CDS             2086..>2176
                     /locus_tag="PKV23_07845"
                     /inference="COORDINATES: protein motif:HMM:NF019998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85384.1"
                     /translation="MSKKAPAKALNAIEGRVIDLGYFGKDSLYR"
BASE COUNT          491 a          678 c          585 g          422 t
ORIGIN      
        1 gtagctgaac ttgtcgaaat tgctcacgcg cagatcatcg ttaggcctga tggcgggtgc
       61 cgctctggcc atgccctaca tcagccgggc ccacgcccag ggagaagccc tcaacgtctt
      121 caactggacc gattatatcg gcgagacgac gattgaggat tttcagtcag ccaccggcat
      181 tctggtcgcc tatgacctct atgactcaac cgaggccatg gaagccaaga tgctggccgg
      241 cagctccggc ttcgatgtgg tggtccaggc cggctccact cttgaacgct tcaccgaagc
      301 aaaggtctat cagccgcttg accgctcgaa actgaccaat tggggcaacc ttgacccggt
      361 aagcctgaaa accgtggagg gctgggaccc tggcaacgtc tacggcgttc cctacatgtg
      421 gggcaccgtg ggcctgacct tcaatgtgga catggtgaag gagcgcattc cggatgctga
      481 cttcaccacc ctcgacattc tcttcaagcc ggaaaatgcc gccaagcttg ccgattgcgg
      541 catttccatc ctggaaagcc cgggcgacgt gattcccatg gcgctggcct atctggggct
      601 tgatcccaac accacgaatc ccgcggactt cgatgcggtg gtcaagcttt tcgctcccat
      661 ccgccagcac atccgggcct tcgacgcctc caactatctg aatgccattc ccaacaagga
      721 actgtgcatg atcaacaact ggtcgggcga ttatgccacc gccaatgccc gtgccgcgga
      781 agccggcatc cagctcaatc tttcctatca tgtgcccaag accggctcgc cggcctggtt
      841 cgatatctgg tgcattcctg ccgacgccaa gcatgtcgat aatgcccaca agttcatcaa
      901 ctacatgatg gagcctgagg tcatcgccaa atgcacgaac tatacaaact atgccaacgt
      961 gaatctcgcg gctgacaagt ttgtcgatcc ggccattctc gccgatccag cggtttaccc
     1021 tgatgctgag ctgaagaagc ggctgtggac ccagaaatcg atgagttcgg acctggaacg
     1081 ggcccgaacg agggcctgga gcaccatcaa gacaggctca ccatcctgac accagagaag
     1141 cccggcaccc tgccgggctg aatttcttgg ggacgcataa tgggcacaga gaataccggc
     1201 aaggcctggg aaaacccgaa agccgagccg ttcatccgga ttgaaaacgt caccaagaaa
     1261 ttcagtgact tcatagcggt caacaatgtg agcctcgata tttacaaggg cgaactgttt
     1321 gccctcctcg gcggctccgg ctgcggcaag acaacgcttc tccgcatgct ctccggattt
     1381 gaatcgccca ccgaaggccg cgtctggatc gatggtcagg acatgaccag cgttccgccc
     1441 tatgagcggc cggtcaacat gatgttccag tcctatgcgc tttttccaca catgacggtg
     1501 gaacagaatg tcggctacgg cctgaagcat gagattctta cgaaggccca gcgccaggac
     1561 cgcgtgaagc agatgcttga tctggtccag ctctcgcagt ttgccgggcg caagccccac
     1621 cagttatccg gcggccagcg ccagcgcgtg gccctcgccc gcgccctcgc caagcagccc
     1681 aaggtcctgc tgctcgacga accgctcggc gccctcgaca agaagctgcg cgagcacacc
     1741 cagtttgaaa tcatgaacat ccaggaaaag accggcatca cctttgtcgt cgtcacccac
     1801 gaccaggaag aggcgatgac gctgtccacc cgcattgccg tcatggacaa gggcatcatc
     1861 cgccagaccg gcacccccac cgaaatctat gagtttccca agaccaaatt cgtcgccgat
     1921 ttcatcggtt cgatcaacca gtttgccggc acggtaaaat ccgtgaccaa ggacaaggct
     1981 gtcgtggcct gcgctgattg gccgggagac ataaatgttg accgcgatgc cacactggaa
     2041 gtcggccaga atgtttccgt ggccgtccgc ccggaaaaaa tctcgatcag caagaaggcg
     2101 cccgcaaagg ccctcaatgc gatcgaaggc agggtcatcg atcttggtta cttcggcaag
     2161 gattcgcttt accggg
//
LOCUS       DAORVN010000375         2174 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_52626_length_2174_cov_3.511090, whole genome shotgun sequence.
ACCESSION   DAORVN010000375 DAORVN010000000
VERSION     DAORVN010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2174
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52626_length_2174_cov_3.511090"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(90..1076)
                     /locus_tag="PKV23_07850"
     CDS             complement(90..1076)
                     /locus_tag="PKV23_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018182735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85385.1"
                     /translation="MFRLDQKLSRIRAGKYKRSDFIIADAKDGDMGPSIPGCGPKRNK
                     DGSSTRFVTRPEFLENIAAIVKQNVVDIMLVSASNLEALADKGVFKKSAVKPAIRAND
                     TTDIWVGRGATYSKQASRPFRSASLSRVKKYTDLGLYSITFNNDLDQDYNALEHFSAF
                     REDAARNKFNYFLEVFNPNVECGIDPALVPHYINDSIIRCLAGVTKADRPQFLKIAYN
                     GPKALEELASFDPSITVGVLGGGAGTTRDCFELLNQAEKYGARVALFGRKINLAEDPL
                     AMVSFMREVASGNIAPLEAVKAYHASLKKSGLTAIRELAADSEITEKVLKAG"
     gene            1288..>2174
                     /locus_tag="PKV23_07855"
     CDS             1288..>2174
                     /locus_tag="PKV23_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018182730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HQX85386.1"
                     /translation="MTDYATEKERASLANASDILSLIASGTANSRAALLAASGMSRVT
                     VTQRLNVLLGVGIVEETLRTLPSGGRPTRVLGIRGSAGYMLVADIGETHIHLAAMSLA
                     PDLLAQSTIAFSIAEGPVATLQRMTGEFDRLAAGLGQGFLVAVSLSMPTPVDFKRGRV
                     VGPSVLYGWDDFDIVSWLGRHYKAPIYVENDVNLMTIYEHRHNFPLVDDMIFIKAGTG
                     IGSGIIAGGKIFRGAQGAAGDIGHIQFNSPDAPLCRCGKLGCVEARAGGWAIARDLAE
                     KGLRAETARDVIALVEMQKP"
BASE COUNT          437 a          610 c          625 g          502 t
ORIGIN      
        1 ttctcccggc caatgcgtca ccatgcggtt gttgtcaacg caccatgtgc cgcccgtgat
       61 aaagccgcgg cgctgggttt cacccatgat tagcctgcct tcaggacctt ttcggtgatc
      121 tcgctgtcag cggccagctc acggatggcc gtcaggccgc tctttttcag gctggcgtga
      181 taggccttga ccgcttcaag cggcgcgatg ttgccgcttg ccacttcgcg catgaaggac
      241 accatggcca gcggatcttc cgccagattg atcttgcggc cgaagagggc gacccgtgcg
      301 ccgtattttt cggcctggtt caaaagctcg aaacagtcgc gcgttgtccc ggcaccgccg
      361 ccgagcacgc caaccgtaat gctgggatcg aatgatgcca gctcttccag ggctttcggg
      421 ccgttgtagg caatcttgag gaattgcggc cggtcggcct tggtcacgcc ggcgaggcag
      481 cgaataatac tgtcattgat gtaatggggg actagcgccg gatcgatgcc gcattcaacg
      541 tttggattaa acacttccag gaagtagttg aatttgttgc gcgccgcgtc ctctcggaag
      601 gcgctgaaat gttcaagtgc gttgtaatcc tgatccagat cgttgttgaa ggtgatggag
      661 taaaggccca ggtccgtata cttcttcacg cgcgaaagcg aagcgctgcg gaatgggcgg
      721 ctcgcctgct tggaataggt agcgccacgc cccacccaga tatccgtggt gtcattggca
      781 cggatggcag gtttgacggc acttttcttg aaaacgccct tgtccgccag ggcctccagg
      841 ttggaggccg aaaccagcat gatatcaacg acgttctgtt tgacgatcgc agcgatgttc
      901 tccaggaatt ccggccgggt cacgaagcgt gtcgaactgc cgtccttgtt gcgctttggc
      961 ccgcaaccgg gaatgctcgg acccatatcg ccatccttgg catccgcaat gataaagtcg
     1021 ctgcgcttgt atttgccggc gcggatacgc gagagttttt gatcaagtct gaacattttt
     1081 tcctgcaggt ttggggttgc gatggtgagg gaacacgatg ctcaacaata ggcgcacatt
     1141 ttgcatatag tcaatcatat ctttgcattt ttatgtatat ttttttacat aattgagcta
     1201 ttccccttat aagttgacca atattctgac agtttcccgt ttgctagtaa tgatccattc
     1261 catgctgtga gcggggagca aagctgcatg acagactatg cgactgaaaa ggagcgggcc
     1321 agcctcgcaa atgccagcga tattctatcg ctgattgcaa gcggcactgc caattcgcgc
     1381 gctgccttgc ttgcggcttc gggcatgtcc agggtgaccg taacccagcg gcttaatgtg
     1441 ctgctgggcg ttggcatcgt cgaagagacg ttgcgcacct tgccaagtgg cggaaggccc
     1501 acgcgtgtcc tcggaatcag gggatcggcc gggtacatgc tcgtagccga tataggtgaa
     1561 acccacatcc acctcgccgc catgtctctc gcgccagacc ttctggcgca aagcacaatc
     1621 gctttcagca ttgcggaagg gcccgtcgcc accctgcagc gcatgaccgg agaattcgac
     1681 aggcttgcgg ccggtttggg ccagggattt ctagttgccg tcagcctcag catgccaacc
     1741 ccagtggatt tcaagcgggg cagggtcgtg ggcccatccg tactctatgg ctgggacgac
     1801 ttcgatatcg tttcatggct gggccggcac tacaaagcgc cgatctacgt tgaaaacgac
     1861 gtcaacctga tgaccatcta tgagcatcgc cacaactttc cccttgtcga tgacatgata
     1921 ttcatcaaag cgggcaccgg cattggcagt ggcatcatcg ccggcgggaa aattttccgc
     1981 ggggctcagg gagcggccgg tgacattggc cacattcagt tcaattctcc ggatgcaccc
     2041 ctctgccgat gcggcaaatt gggctgcgta gaggcccgtg ccggcggctg ggccattgcc
     2101 cgcgatctcg ccgaaaaagg cctgcgagcg gaaaccgcgc gcgacgtcat tgcccttgtc
     2161 gaaatgcaaa agcc
//
LOCUS       DAORVN010000376         2173 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_52676_length_2173_cov_3.124174, whole genome shotgun sequence.
ACCESSION   DAORVN010000376 DAORVN010000000
VERSION     DAORVN010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2173
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52676_length_2173_cov_3.124174"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..376)
                     /locus_tag="PKV23_07860"
     CDS             complement(<1..376)
                     /locus_tag="PKV23_07860"
                     /EC_number="1.17.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009493249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HQX85387.1"
                     /translation="MVGQVQVGGGAPIVVQSMTNTDTADIEGTARQTADLAKAGSELV
                     RITVDRDDAARAVPHIRDRLDQMGVSVPLVGDFHYNGHTLLNDHPACAEALAKYRINP
                     GNVGFKEKKDRNFSSMIETALKF"
     gene            complement(438..1238)
                     /locus_tag="PKV23_07865"
     CDS             complement(438..1238)
                     /locus_tag="PKV23_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018066929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HQX85388.1"
                     /translation="MTSILLEPFFQRAMLAGLAVALVSGPVGCFIVWRRMAYFGESLA
                     HSGLLGVGLGLLFSFNVTLGAVVTAIVFALLLLALRRRHSLATDTLLGILSYTSLALG
                     LIVVGLATGAASDHMDILFGDVLTVSTNDVWAVWIGAAVVLASLAFLWRDLIASSVHE
                     ELARAEGVRVARVELGFILLIAVMTAIAMKIVGLLLITALMVIPAAAARRLATTPESM
                     AFIAAAIAGLSVISGLLASAFVGGASGPCVVLAASCFFALTLAVPQAS"
     gene            complement(1245..2012)
                     /locus_tag="PKV23_07870"
     CDS             complement(1245..2012)
                     /locus_tag="PKV23_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007696673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HQX85389.1"
                     /translation="MLKANDALLSARDVTLNLNGRLILDRVNLDVRRGEIVTVIGPNG
                     AGKSTLVRVMLGLRPLTSGRIERLANLNVGYVPQKFPQTANVPMDVKRLLSLTLKPSN
                     QDIDRALEETGILHLKNARVPSLSGGELQRALLARALLRRPDLLVLDEPVQSVDFIGE
                     MKLYELISGIRKTHGCGILMVSHDLHMVMAESDHVVCLNGHVCCEGGPEKVQQDPEFA
                     KLFGPSAARMIAAYAHHHDHDHEPHDHHHHHHHDHRH"
BASE COUNT          460 a          619 c          711 g          383 t
ORIGIN      
        1 ggaatttgag cgcggtttca atcatgctgg agaagttgcg gtctttcttc tccttgaagc
       61 ccacattgcc ggggttgatg cggtatttgg caagcgcttc ggcgcaggct ggatgatcat
      121 tgagcaatgt gtggccattg taatggaagt ctcccaccag cggcacggaa acgcccatct
      181 ggtccagccg gtcacggatg tggggcacgg ccctggccgc gtcatcgcgg tccacggtaa
      241 tgcggacaag ttcggagccg gctttggcga ggtccgcagt ctggcgggcg gtgccttcga
      301 tatcggcggt gtccgtgttg gtcatggact ggacgacgat gggcgcgccg ccgcccacct
      361 ggacctggcc caccatgaca gcatgggatt ttcgccgcgt ttctctggcc gaataggaca
      421 tggttgctcc ctgctcccta actggcctgc ggcacagcca aggtcaaggc gaagaaacaa
      481 cttgccgcca gaacaacgca aggaccggaa gcgccgccga caaaggcgga ggccagaaga
      541 ccgctgatca ccgaaaggcc ggcaatggct gcggcgatga aggccatgct ttccggcgtt
      601 gtggccaggc ggcgggcggc ggcggcgggg atgaccatca gggcggtgat gagcaggagg
      661 cccacgattt tcatggcgat ggcggtcatc acggcgatga gcaggatgaa gccgagttca
      721 acccgcgcaa ccctgacgcc ttcagcgcgg gccaattcct catgcacgga gcttgcaatc
      781 aaatcacgcc acaggaaagc caagcttgcg agcactacag cggcgccgat ccagacagcc
      841 cagacatcat tagtggaaac ggtcagcaca tcgccgaaca ggatatccat gtgatcggac
      901 gcggccccgg tggcaaggcc aaccacgatc aggccaagag ccagggacgt ataagacaat
      961 atgccaagca gtgtgtcagt ggccaggctg tgccggcggc gcagggcgag aagaagcagt
     1021 gcgaagacga tggctgtgac aacagcgccg agtgttacgt tgaagctgaa gagcaatcca
     1081 agccccacac cgagcaggcc ggaatgggcc aggctttcgc cgaagtaggc catgcgccgc
     1141 cacacgatga aacagccgac aggtccagag acaagggcca ccgcgaggcc tgccagcatg
     1201 gcacgctgaa agaaaggctc gagaaggatg gaggtcatag ccgctcagtg cctgtgatca
     1261 tggtgatgat ggtgatgatg gtcgtgcggc tcgtggtcat ggtcgtggtg gtgggcgtag
     1321 gcggcgatca tgcgtgcggc cgaggggccg aagagcttgg cgaattccgg atcctgctgg
     1381 accttttccg gccccccctc gcagcagaca tggccattga ggcagaccac atgatcgctt
     1441 tcggccatga ccatatgcag gtcgtggctc accatcagaa tgccgcagcc atgggttttg
     1501 cgaattccgg aaatcagttc gtagagcttc atttcaccga tgaagtccac ggactggacc
     1561 ggttcatcga gaaccagcag atcgggccgg cgtagcaggg cgcgggccag cagcgcgcgc
     1621 tgcaactcgc cacccgaaag ggatggaacg cgggcgttct tcagatgcag aattccggtt
     1681 tcctccaggg cacggtcgat atcctgattg cttggtttca gggtgagcga caggagacgc
     1741 ttcacatcca tcggtacgtt ggcggtttgc ggaaacttct ggggcacata gccgacattc
     1801 aggttggcca gacgctcgat gcggccagag gtcaagggac gaaggccaag catcacgcgc
     1861 accagggttg actttcccgc gccattggga ccgatgacgg tgacgatctc gccgcggcgc
     1921 acatccaggt tcacgcggtc aaggatcagg cggccgttga gattgagcgt cacatcgcgg
     1981 gcgctcaaca acgcgtcatt ggctttcagc atgtcaggcg gccctcgcgc aggcgccgca
     2041 ggtgcccgac atctcaatca tcgtatgggc cggggaaaat ttctgggccc tggccttcgc
     2101 ggtgatgcgt tcaaatattt ccggcgcatc aagttccgag accgtgccgc aggcctcaca
     2161 gatcagcagg gcg
//
LOCUS       DAORVN010000377         2169 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_52873_length_2169_cov_2.398297, whole genome shotgun sequence.
ACCESSION   DAORVN010000377 DAORVN010000000
VERSION     DAORVN010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2169
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52873_length_2169_cov_2.398297"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..382
                     /locus_tag="PKV23_07875"
     CDS             <1..382
                     /locus_tag="PKV23_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009489496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HQX85390.1"
                     /translation="AHICHCRMCQKAFGNLFAALAGVNKADVSWTLGEPAYFRSSSIV
                     QRGFCRHCGTPLTFAYDHTPHMNISIGSLDNPALAKVETQYGMEGMTQAFSALHALPG
                     SRTEDDISAEDLRRLKSFQHPDHD"
     gene            complement(379..>2169)
                     /gene="alaS"
                     /locus_tag="PKV23_07880"
     CDS             complement(379..>2169)
                     /gene="alaS"
                     /locus_tag="PKV23_07880"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012651592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HQX85391.1"
                     /translation="VLPANEGRGYVLRRIMRRAMRHAELLGQREPLMWKLVPTLVHEM
                     GAAYPELQRTQDLITETLRLEETRFRKTLERGLRILDEESAGLKTGDVFAGDTAFKLY
                     DTYGFPLDLTQDALRSRGVVVDTDAFSKAMEVQKTEARRAWKGTGDAKADAIWFEIRD
                     AVGTTEFLGYETEKAEGVITALVADGKQVKSLNAGDKASIVVNQTTFYAESGGQTGDH
                     GIIKSASGGVFRVTGTEKRLGDLVIHMGQVESGTLALGDAVELHVDHARRSGNRQHHS
                     ATHLLHEALRKTLGGNVAQKGSLVEPNRLRFDFSHNKGMSHDELRQVEAMANDIVLQN
                     DPVNTRLMSVDAAVAEGAMALFGEKYGDEVRVVSMGRNPEGASRPIYSLELCGGTHVS
                     RTGDIGLIKILAESGSAAGVRRLEALAGTAARDYLEQQDKRVAAVAAVLRTTPAELVS
                     RVEHLVDERRKLERELAEAKKALALGGGASAGDSDVETINGIKFMKRVLSDMDSKDMK
                     GLVDDGKARLGSGVVALLSTNAEGRASLYVGVTADLTAKLSAVDLVRAGAAVLGGKGG
                     GGRPDLAQAGGPDAGKADEALAAIRTVIAG"
BASE COUNT          380 a          740 c          616 g          433 t
ORIGIN      
        1 cgcccatatt tgccattgcc gcatgtgcca gaaggctttc ggaaatctct ttgcggcact
       61 ggccggcgtg aacaaggccg atgtcagctg gaccttgggc gaaccggcct atttccggtc
      121 gtcgtccatc gtgcagcgtg gcttctgccg gcactgcggc acgccgctca ccttcgccta
      181 tgatcacacg ccccatatga acatctcgat tggctcgctg gataatccgg cactcgcaaa
      241 agtggaaact caatatggca tggaaggcat gacgcaggct ttttccgcgc tccacgcgct
      301 gccgggctca cgcaccgaag acgacatttc agccgaagac ctgcggcggc tgaaaagctt
      361 tcagcatccc gaccatgact agccggcaat caccgtcctg atggccgcga gcgcttcatc
      421 agctttcccg gcatcggggc cgccagcctg tgccaggtcc ggccgtccgc cgccgccttt
      481 gccgccgagc acggcggcgc cggcccggac caggtcgacc gcgctgagtt ttgccgtcag
      541 gtccgccgtc acccccacat agaggctggc ccggccctca gcatttgtgc tgagcagtgc
      601 caccacgcct gaaccaagcc tggccttgcc gtcatcgacc aggcccttca tgtcctttga
      661 gtccatgtcg ctcaaaacgc gcttcatgaa cttgattcca ttgatggtct ccacgtcgct
      721 gtcgccggcc gaggcacccc cgcccagagc gagggccttc ttggcttccg caagttcgcg
      781 ttccagcttc cggcgctcat ccaccaggtg ctcgacgcgg ctcaccaact ccgccggtgt
      841 ggttctgaga accgctgcca ctgctgcaac gcgtttgtcc tgctgctcga ggtagtcgcg
      901 cgccgccgta cccgccagtg cttcaagccg ccggacgcct gcagccgaac cgctttccgc
      961 gaggatcttg ataaggccga tgtcgccggt gcgcgacaca tgcgtgccgc cacagagttc
     1021 cagcgagtag atcggccggc tggcgccttc cggattgcgg cccatggaca cgacgcgcac
     1081 ttcatcgccg tatttttcgc caaacaacgc catggcgccc tcggcaaccg ccgcatctac
     1141 cgacatcagc cgcgtgttga ccggatcatt ctgcaggaca atgtcatttg ccatggcctc
     1201 gacctggcgg agctcatcat ggctcatgcc cttgttgtgg gaaaagtcaa agcgcaggcg
     1261 gttgggctcg accagcgaac ctttttgcgc cacgttgcca cccaaggtct tgcgcaacgc
     1321 ctcgtgcagc agatgcgtgg ccgaatggtg ctggcggttg ccggaacggc gggcatgatc
     1381 gacgtggagc tccaccgcgt cgccaagcgc cagtgttccc gattctacct ggcccatgtg
     1441 aatgaccagg tcgcccaggc gcttttccgt acccgtcacc cggaacaccc cgccgctggc
     1501 ggacttgatg attccatgat caccggtctg cccgccggac tccgcataga aggttgtctg
     1561 attgacgaca atcgaggctt tgtctccggc attgagtgac ttcacctgct tgccatcggc
     1621 aaccagggcc gtgatcacgc cttcggcctt ttccgtctca tagccgagga attcggtcgt
     1681 tcccacggca tcgcggattt caaaccagat tgcatcggcc ttggcatcgc ccgtgccctt
     1741 ccaggcccgg cgcgcttcgg tcttctgaac ctccatggcc tttgaaaaag cgtccgtatc
     1801 cacaaccacg ccgcgcgagc gcagcgcgtc ctgggtgaga tcaagcggaa agccataggt
     1861 gtcatagagc ttgaaggcgg tgtcgcctgc aaacacatca ccggtcttca gtcccgcgga
     1921 ctcctcatca agaatgcgaa gcccccgctc cagggttttg cggaagcgcg tttcctcaag
     1981 gcgaagggtt tccgtaatca gatcctgcgt gcgttgcagc tccggatagg cagcacccat
     2041 ctcatgaacc agggtgggaa ccagtttcca catcaggggt tcccgctggc ccaggagttc
     2101 ggcatggcgc atggcgcgcc gcatgatccg ccgcagcacg tagccgcgcc cctcgttggc
     2161 gggcagcac
//
LOCUS       DAORVN010000378         2167 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_52954_length_2167_cov_2.696496, whole genome shotgun sequence.
ACCESSION   DAORVN010000378 DAORVN010000000
VERSION     DAORVN010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52954_length_2167_cov_2.696496"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            241..486
                     /locus_tag="PKV23_07885"
     CDS             241..486
                     /locus_tag="PKV23_07885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85392.1"
                     /translation="MHTPFTMDDLVSRNMNPEKLETLRRVFDTVCAEAAIPESAKSER
                     NELAEKLLTAGVTVGETPEYETLLMTYARRVVAHYRH"
     gene            767..988
                     /locus_tag="PKV23_07890"
     CDS             767..988
                     /locus_tag="PKV23_07890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85393.1"
                     /translation="MINPNEKYILQFPVNDGSCMNDNGFAYFESQARKAAAQKGCVLV
                     GLNNVRLEFVRGRWSVSGTAILEKLQATG"
     gene            1272..1661
                     /locus_tag="PKV23_07895"
     CDS             1272..1661
                     /locus_tag="PKV23_07895"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQX85394.1"
                     /translation="MEEQKLPSMGARRVVIIDDEAAFAKALTRIVNALGYEVTVSTDA
                     RSSYTFDLKSSDIVFVDVLMPNVSGLEVLQKLAQQKTRSAIVLMSGHEERLDEAEKLA
                     RNLDLNLVGALTKPFRLEDVKDVLLGA"
     gene            complement(1667..2110)
                     /locus_tag="PKV23_07900"
     CDS             complement(1667..2110)
                     /locus_tag="PKV23_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011156341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HQX85395.1"
                     /translation="MLIFILNILWFVLGGFFAGLGWILAGVIMAITIIGIPWARSCFM
                     LARYTFWPFGYDIVSREQLYGEPDIGTGTLGTIGNIIWFVLAGWWLAIMHITAAAALA
                     ITIIGIPFAWAHVKLALASLFPIGKTVVEIDELKLLPGAGTGLRV"
BASE COUNT          472 a          623 c          673 g          399 t
ORIGIN      
        1 cggcgcggct gcgccaggcg aaggacgccc agatcaacat gcgcctgcca tcaaaattgc
       61 tggccgcagt caaggcaagc gccaagagca gcggcatccc ctatcagcgc ttcatccgtg
      121 aaacactgga acaagccgtg gggaaaaagc gggcgtaaca tacgctatgt tttggtgcgg
      181 gaacttttga atattcgcgg cgttcaactc tcctgaatcc ggcagcgaaa ggaggcccgc
      241 atgcacacgc cattcacgat ggatgatctg gtgtcccgca acatgaaccc cgagaagctg
      301 gaaaccctgc gccgcgtctt tgatacggtg tgcgcggaag cggcgatccc cgaaagcgcc
      361 aagagcgagc gcaacgaact ggccgagaag cttctcaccg ccggcgtgac ggtgggcgaa
      421 acgccggaat atgaaaccct gctgatgacc tatgcgcgcc gcgtggtggc gcactaccgg
      481 cactgacaac gccgcggaac agtttcccgc cgggcgcatt ctgtaaaggc atgaccaggc
      541 gtcgtgtggc gtgccggtcc gggttcccct cgtccggcag ccggaagccc cgtcgctgac
      601 gagcccgggg cttctttttt cctccagcga tggaactttc gcgtgcccct tgcgttgatt
      661 tgcgcgctgg gcccaaagcg cccgccaggc aacggaacag aaaaagagac tggccaaaat
      721 cctgtcacaa attgggcgat gatgtgcgca agtgttggag gccgacatga tcaacccgaa
      781 tgaaaaatat atcctgcagt ttccggttaa cgacggctcg tgcatgaacg acaatggttt
      841 tgcctatttt gaatcgcagg cccgcaaagc ggcggcgcaa aagggctgcg ttctggtagg
      901 cctgaacaat gtcaggctgg aattcgtgcg cggccgctgg agcgtgagcg gcacagcaat
      961 tctcgaaaag ctccaggcaa ccggttaagg cccgttgctg gcaagagcgc ctgccactat
     1021 ctcggaatgg gcccaggcgg gtctggaaca ggccgcggat ccgggttggg ctgcgggtgc
     1081 ggaatggggt tgggccgcgg gttggttccc atcggaaagc tccttctcat gctttaggta
     1141 gaatgtggtt gcagcccatt ggttcattgt ccgccgctcc ctcaaacctc tgtgactttc
     1201 acattaaagt gtaggactcg gccccgtttc gtgcgtagaa tcccgactca tgcaggcaag
     1261 ggatttgttc cgtggaagaa cagaaattgc catcaatggg agcccggcgc gttgtcatca
     1321 tcgacgatga agcggccttc gcgaaagcct tgacccggat agtcaatgcc ctgggttatg
     1381 aggtgacggt ttccactgac gcaaggtcaa gctacacctt tgacctgaag agttccgata
     1441 ttgtttttgt tgacgtgctg atgcccaacg tctcggggct ggaggttctg caaaagctgg
     1501 cgcagcagaa aaccagatcg gcgattgtgc tgatgagcgg ccatgaggaa cgccttgacg
     1561 aggccgaaaa actggcgcgg aacctggacc tgaatctcgt cggcgcgctc accaagccgt
     1621 tccgcctcga ggacgtgaag gacgtgcttc tcggcgcctg agggattcag acccgcaagc
     1681 cggtccccgc ccctggcagc agcttcaact cgtcgatctc caccaccgtc ttgccgatcg
     1741 gaaacagcga ggcgagcgcc agcttcacat gcgcccaggc gaagggaatg ccgatgatgg
     1801 tgatggcaag ggccgccgcc gccgtgatgt gcatgatggc cagccaccag cccgccagca
     1861 cgaaccagat gatgttgccg atggtgccga gagtgcccgt gccgatgtcg ggctcgccat
     1921 agagctgctc gcgcgagacg atgtcatagc cgaagggcca gaaggtgtag cgcgccagca
     1981 tgaagcagga gcgcgcccag ggaatgccga tgatggtgat ggccatgatc actccggcca
     2041 ggatccagcc gaggccggcg aaaaagccgc caagcacaaa ccagaggatg ttgaggatga
     2101 aaatcagcat gtgccttgcc ctcctgagcg ggactgcaaa taggaacgcc ctgcccctgt
     2161 ttcaagg
//
LOCUS       DAORVN010000379         2165 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_53026_length_2165_cov_3.194313, whole genome shotgun sequence.
ACCESSION   DAORVN010000379 DAORVN010000000
VERSION     DAORVN010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2165
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53026_length_2165_cov_3.194313"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="PKV23_07905"
     CDS             <1..199
                     /locus_tag="PKV23_07905"
                     /inference="COORDINATES: protein motif:HMM:NF016532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF599 family protein"
                     /protein_id="HQX85396.1"
                     /translation="EAVKSLNNGIRGYYFAIAAFFLFAGPYFCMAMTVVMSTLLLYRQ
                     GFSTTALAIERYVDAMGDIKK"
     gene            complement(200..841)
                     /gene="eda"
                     /locus_tag="PKV23_07910"
     CDS             complement(200..841)
                     /gene="eda"
                     /locus_tag="PKV23_07910"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019964500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008675 -
                     2-dehydro-3-deoxy-phosphogluconate aldolase activity
                     [Evidence IEA];
                     GO_function: GO:0008700 - 4-hydroxy-2-oxoglutarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0009084 - glutamine family amino acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HQX85397.1"
                     /translation="MTRDLQAGMEAILSAAPVVPVMVIERVADAVPLAKALVKGGLPV
                     LEITLRTAAAIESIRAIMAEVKGAIVGVGTVITPAQMAETDRLGCAFAVSPGSSPLLI
                     KAARDFRMPLLPGGVTASECMNLLEHGYSFQKFFPAEPAGGAAYLASLAQPLPQIRFC
                     PTGGITLESAPRYLKLANVITVGGSWMVPKKLIEAGDWAAIEALARQASSLKG"
     gene            complement(841..>2165)
                     /gene="edd"
                     /locus_tag="PKV23_07915"
     CDS             complement(841..>2165)
                     /gene="edd"
                     /locus_tag="PKV23_07915"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012113721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HQX85398.1"
                     /translation="LSFGHLPAVFIPAGPMTSGLPNDDKAKVRQLYAQGKVDRSALLE
                     AEAQSYHGPGTCTFYGTANSNQMLMEIMGLHLPGSTFLNPGTALRDEITKASAQQALS
                     ITALGNSYTPIGRIYDEKAVVNGVVGLLATGGSTNHTLHLIAMAAAAGIMLTWKDLAE
                     LSQVVPLMTRIYPNGKADVNHFNAAGGMGFLIRELLHRGVLHEDVTTIMGTGLNGYLV
                     EPRLSHDGALQWQDAASVSHDDTVLRGCENPFQVTGGLNVLEGPLGHAVIKTSSIPGD
                     RHFIEAPARVFHSQEELHAAFKTGELHRDVVAVVRFQGPKANGMPELHRMTPPLSVLQ
                     DKGFRVGLVTDGRMSGASGKVPAAIHVTPEAADGGAIAKIRDGDVIRIDADNGRLEIL
                     VDAREFAARVPATADLSKNEEGTGRELFKAFRNLAGRADQGASIFEVA"
BASE COUNT          429 a          618 c          687 g          431 t
ORIGIN      
        1 cgaagcggtg aaaagcctca acaacggcat tcgcggctat tacttcgcaa tcgccgcttt
       61 cttccttttc gccgggccct atttctgcat ggccatgacg gtcgtgatga gcacgctgct
      121 gctctaccgg cagggatttt ccaccacggc gctggccatc gaacgctatg tcgatgcgat
      181 gggagacatc aagaagtaat cagcccttga gactggaggc ctggcgggcg agcgcttcta
      241 ttgcagccca atcgccagcc tcgatcagtt tcttcggcac catccatgaa ccgcccacgg
      301 taatgacgtt tgcaagcttc aggtatctcg gtgcggattc cagggttatt ccgccggtgg
      361 ggcagaagcg gatttgcggc aggggctggg ccagcgatgc cagataggcg gcaccgcctg
      421 cgggttcggc ggggaagaat ttctggaagc tgtagccgtg ctccaaaaga ttcatgcatt
      481 ccgatgcggt aacaccgcca ggcagcagtg gcattctgaa atcccttgcc gccttgatca
      541 gcaggggcga ggatccaggc gacacggcaa aggcgcagcc cagcctgtcc gtctcagcca
      601 tctgggcagg cgtgatcacc gtgccgacgc cgacaatggc gcctttgact tccgccatga
      661 tcgcgcggat ggactcgatg gcggcggcgg tacgcagggt gatttcgagg accggcagac
      721 cgcccttaac aagtgccttg gccaacggta ctgcatcggc aacccgttca atcaccatca
      781 cgggcactac gggagctgcc gagagaatgg cttccatgcc tgcctgtaaa tcccttgtca
      841 tcatgcaacc tcgaaaatcg atgcgccctg gtcggcgcgg ccggcaagat tccggaaggc
      901 cttaaacagc tcgcggcccg tgccttcctc attttttgaa agatcggcgg tggcaggaac
      961 gcgtgccgca aattcccttg catccaccag gatttcaagg cggccgttgt ccgcgtcgat
     1021 gcggatgaca tcgccatccc ggattttggc tatggcgccg ccatcggcgg cctcgggtgt
     1081 cacatgaatg gcggccggca ccttgccgga agcgccggac atgcggccat cggtgaccag
     1141 ccccacgcgg aatcccttgt cctgcaacac gctgagcggc ggcgtcatgc ggtggagttc
     1201 cggcatgcca ttggccttcg ggccctggaa acgcaccacg gcaaccacgt cgcggtggag
     1261 ttcgcctgtc ttgaaggcag cgtggagttc ttcctgcgag tgaaaaacgc gggcgggcgc
     1321 ttcaatgaaa tggcggtcac cggggattga agaggtcttg attacggcat ggccgagcgg
     1381 gccctcgagc acattgagtc cgccggtaac ctgaaacgga ttttcgcagc cgcgcaatac
     1441 cgtgtcgtca tgggaaacgg aagcggcgtc ctgccattgc aacgcgccgt catggcttag
     1501 cctgggttca acgagataac cattcaggcc cgtacccatg atggtggtga catcctcatg
     1561 gagcacgcca cggtgaagta attcgcggat gagaaatccc atgccgccgg cggcgttgaa
     1621 atgattcacg tcggccttgc cgttgggata gatgcgggtc atcaggggca cgacttggga
     1681 aagttcggcg agatccttcc aagtcagcat gatgcctgct gcagcagcca tggcaatgag
     1741 atggagcgtg tgattggtgg agccgccggt cgccagcaag ccgacaacgc cgttcaccac
     1801 ggccttctcg tcatagatgc ggccaatggg tgtgtagctg ttgccaagcg ccgtgatcga
     1861 aagcgcctgc tgggcggagg ctttggtgat ttcgtcacgg agagccgtgc cgggattcag
     1921 gaaggttgat ccgggaaggt gaaggcccat gatttccatc agcatctggt tggaattggc
     1981 ggtgccgtaa aacgtgcagg tgccggggcc atggtaggat tgcgcttcgg cttccagcaa
     2041 cgcagagcgg tcaaccttgc cttgcgcata gagttggcgg accttggcct tgtcatcatt
     2101 gggcaggccg gatgtcatgg ggccggcggg aatgaacacg gcgggcaaat ggccaaatga
     2161 caatg
//
LOCUS       DAORVN010000380         2151 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_53627_length_2151_cov_3.004771, whole genome shotgun sequence.
ACCESSION   DAORVN010000380 DAORVN010000000
VERSION     DAORVN010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53627_length_2151_cov_3.004771"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..772)
                     /gene="dusB"
                     /locus_tag="PKV23_07920"
     CDS             complement(<1..772)
                     /gene="dusB"
                     /locus_tag="PKV23_07920"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011473023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HQX85399.1"
                     /translation="MNIPIGPIKTRNRVFLAPLSGGTDEPFRAVAHAHGAGLVVSEMV
                     ASEELVRARPDMVRRARGTHRLKPFVIQLAGREARWMTEGAKVAQDLGADIIDINMGC
                     PARQVTGGLSGSALMRDLDHAVTLIEATVQAARVPVTLKMRLGWDANSMNAPELARRA
                     ENAGIQMITVHGRTRCQFYGGRADWRAIRAVKEVISIPLIANGDGTSAADARAMLAQS
                     HADGVMIGRGAYGRPWWPGVIANQLEPGSGIDEPSLHQE"
     gene            882..2051
                     /locus_tag="PKV23_07925"
     CDS             882..2051
                     /locus_tag="PKV23_07925"
                     /EC_number="2.7.7.60"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase/2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase"
                     /protein_id="HQX85400.1"
                     /translation="MSVAVVIVAGGAGFRAGGELPKQYQLIGGKPVIWWTLKAFAEHP
                     GISHVQTVIGAGHENLFAAAIGDLRVAPPVVGGATRQDSCRIGVAACSSAGVKRVLIH
                     DAARPFVSADLISHVIAELERSESVIPGLPIADTIKHAPGGLVERTVDRQSMWSIQTP
                     QGFDLAIILAAHTKAHEQNLLGLTDDAAVAEKFGIKVRVIAGRTENRKLTTAEDVHEA
                     DRILTAMANSERLDVRVGQGIDFHEFEAGDGVVLCGVKIPHDKKLKGHSDADAAMHAL
                     TDAILGALGEGDIGSHFPPSDPQWKGAASSTFLKKAVDLMTSRNGIIANVDITILAET
                     PRVGPHIEAMKTLLSPLLHLSKDRIAIKATTTEQLGAIGRREGLAAFAMATVRLP"
     gene            2048..>2151
                     /locus_tag="PKV23_07930"
     CDS             2048..>2151
                     /locus_tag="PKV23_07930"
                     /inference="COORDINATES: protein motif:HMM:NF014517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HQX85401.1"
                     /translation="MNFELRAAALLDKCRASGVKIATAESCTGGMVAA"
BASE COUNT          442 a          606 c          668 g          435 t
ORIGIN      
        1 tttcctggtg cagtgatggt tcgtcgatgc ctgaaccggg ttcaagttga ttggcaatta
       61 cgcctggcca ccagggcctg ccataggcgc cgcggccgat catgacgcca tcggcatggg
      121 attgcgccag catggcacgg gcgtccgcgg cagaggttcc gtcaccattg gcaatcaggg
      181 gaatgcttat gacttccttg acggcacgaa tggcgcgcca gtcagccctg ccgccataga
      241 actggcaacg ggttcgcccg tgtacggtga tcatctgaat acccgcattt tcggcgcggc
      301 gtgccagttc gggcgcgttc atcgagtttg catcccagcc cagacgcatc ttgagggtga
      361 cgggcacccg ggccgcctga acggtggcct cgatcagcgt cacggcatgg tcaagatcgc
      421 gcatcagggc agaccctgag aggccgccag taacctgccg tgccggacag cccatgttga
      481 tatcgatgat gtccgcgccc agatcctggg ctactttggc gccctccgtc atccagcgcg
      541 cctcgcggcc tgcaagctgg atcacaaagg gcttcagcct gtgggtgccc cttgcgcggc
      601 gcaccatatc gggccgggcc ctcaccagtt cctcactggc gaccatttcc gacacaacaa
      661 gcccggcccc atgggcatga gccacggcac gaaagggttc atccgtccct cccgaaaggg
      721 gagccaggaa aaccctattg cgggttttga tggggccgat tggaatgttc attttttaat
      781 cacgcgccca gctagcatat tttataagca gaatagacct atgttgcact gcgtcaagac
      841 catttattgc cccatggcgg agggactgct agacgaaaat tatgtcggtt gcagttgtca
      901 tcgttgctgg aggtgcggga tttcgtgccg gcggggaatt gccgaaacaa tatcagctca
      961 ttggcggaaa accggtcatt tggtggacac tgaaggcatt tgccgagcac ccggggattt
     1021 cgcatgtgca aaccgtaatt ggggccggcc atgaaaacct gtttgcagcg gccatcgggg
     1081 atttgcgcgt tgcgccgccg gttgtgggcg gcgccacccg gcaggattcg tgccgcatag
     1141 gggtggccgc ttgcagcagc gcgggtgtca aaagggtcct gatccacgac gcggcccggc
     1201 cttttgtctc cgctgacctg atcagccatg tcattgctga acttgaacgg tccgagagtg
     1261 tgattcccgg cctgccgatc gccgatacaa tcaagcatgc gccgggtggc ctggttgaac
     1321 gaacggttga ccgccagagc atgtggtcga tacaaacacc gcagggtttt gatctggcaa
     1381 tcatacttgc ggcccacacg aaagcccatg agcagaatct gttgggattg acggacgatg
     1441 cggcagtggc agagaaattt ggaatcaagg tgcgggtgat cgccgggcgg accgaaaacc
     1501 ggaagctcac aacagcggaa gacgtgcatg aggcagacag aattttgact gcaatggcaa
     1561 actccgagcg gctggatgtg cgagtcggcc agggcatcga tttccatgag tttgaagcgg
     1621 gcgatggcgt ggttctgtgc ggcgtcaaga ttccccacga caaaaagctg aaaggtcact
     1681 ccgacgcaga tgcggcgatg catgcgctga ccgacgccat tctgggtgca ttgggcgaag
     1741 gcgatatcgg cagccacttt ccaccgagtg atccgcaatg gaagggcgct gcctcttcca
     1801 cattccttaa aaaggcggtg gatctcatga cttcgcggaa cgggatcatt gccaatgtgg
     1861 acatcaccat tctggcggaa acgccgcgcg ttggccctca tatcgaggcg atgaagactt
     1921 tgctttctcc cctgctccac ctttccaagg accgcattgc catcaaggcg acgacgacgg
     1981 agcaattggg tgcaatcggc cggcgcgaag ggcttgcggc atttgcaatg gccacggtgc
     2041 gcctgccatg aactttgaac tgagagctgc ggccctattg gacaaatgcc gcgcgagcgg
     2101 tgtcaagatc gcaacggcgg aaagttgcac cggcggcatg gtggctgcgt t
//
LOCUS       DAORVN010000381         2144 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_53955_length_2144_cov_2.172331, whole genome shotgun sequence.
ACCESSION   DAORVN010000381 DAORVN010000000
VERSION     DAORVN010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53955_length_2144_cov_2.172331"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="PKV23_07935"
     CDS             complement(<1..221)
                     /locus_tag="PKV23_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009028099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85402.1"
                     /translation="MRIPLRFLGALVAVISMLGLGLQSAHADSGKITLTVYKAGWVIG
                     GSGGSGRLTFKGRSYRLGVGGIDYGLVFGG"
     gene            complement(340..1440)
                     /locus_tag="PKV23_07940"
     CDS             complement(340..1440)
                     /locus_tag="PKV23_07940"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HQX85403.1"
                     /translation="MSEALTLPLQNRLSGTAACIIHGFRANAVLYVLAAFVFLVAVAE
                     SIWIGLPFDIKMVTAFTAPVLTLLCGLILIGIVAELVRLYWTGYEGSALGGLWLKISD
                     DYLAPQRISNSIHAFLFMSLYMLGFAFIKKAIPLAVPFSWDVTFMEWDQVMHFGAQPY
                     EWLAPLLNVPWVTFLINVNYNIWFFVMFTCWFWQGFGPKDTALRTHFLLGFTLTWFVG
                     TSVCGTIFSSVGPGLYGRLMPEVQDPFVPLMDWLHEVAQVHTIFVIPTMDELWKDYET
                     GTGLINGISAMPSMHVGTSVLFAILGFSAGKKWLGYLMSVFAFLIFIGSIHLGWHYAI
                     DGYAGAAIAVACWWISGKIVAWDRRKRGVAET"
     gene            1551..>2144
                     /locus_tag="PKV23_07945"
     CDS             1551..>2144
                     /locus_tag="PKV23_07945"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HQX85404.1"
                     /translation="MKSFSDLSEQEIIALAISSEDDDARAYSGFANALMETYPGSAKV
                     FMEMAEEEHQHRRWLTELYQKKFGDFIPLIRRSEINGFVKHKPAWMIGKLNIEAMRRR
                     AEVIELENYRFYKQAAAHSQDPQVAKLLNRLAEAEKGHESLAARLGDKHLTADVKIEE
                     GETKRRLFLLQVIQPGLVGLMDGSVSTLAPLFAAAFAT"
BASE COUNT          528 a          604 c          626 g          386 t
ORIGIN      
        1 ccgccgaaca caagtccgta gtctatgccg cccactccaa gccgatagct gcggccctta
       61 aaggtgagcc tgccgctgcc gcctgaacca ccgattaccc agccggcctt gtagaccgta
      121 agcgtgattt tgccgctatc ggcatgggca ctctggaggc ccaaacctaa catggagatt
      181 actgcgacca aagcgccgag aaaccggagt ggaattcgca tggcttgatt tccttttacg
      241 ttgccggagc atcgatgttg gcaattcccg tctgtccagt gaggacccga ctcgccatgg
      301 ctttaccttg cggacaaagg tgtcaggagt caattcagat caggtttcgg caacgccccg
      361 cttgcgcctg tcccaggcga cgatctttcc tgaaatccac cagcaggcaa cggcaatggc
      421 ggcgccggca tagccatcga tggcgtaatg ccagcccagg tgaatggagc caatgaagat
      481 aaggaaggcg aagaccgaca tgaggtagcc cagccatttc ttgccggcgg agaagccgag
      541 gattgcaaac agcaccgagg ttcccacatg catcgatggc atcgccgaaa tgccgttgat
      601 caggccggtg ccggtctcat agtccttcca cagctcatcc atggtgggga tgacaaaaat
      661 cgtgtgaact tgcgccacct cgtggagcca gtccatgagc ggcacgaaag ggtcctgaac
      721 ctcgggcatc aaccggccat aaaggcccgg gcccacggat gagaaaatcg tcccgcatac
      781 gctggtgccc acgaaccagg tgagggtgaa acccagcagg aagtgggtgc gcagtgcggt
      841 gtccttgggg ccgaagcctt gccagaacca gcaggtgaac atcacaaaga accagatgtt
      901 gtagttgacg ttgatcagga aggtgaccca gggcacgttc agcagggggg ccagccattc
      961 atagggctgc gcgccgaaat gcatcacttg gtcccactcc atgaaagtca catcccagga
     1021 aaaggggacg gccagtggaa tggctttctt tatgaaggca aagcccagca tgtagaggga
     1081 catgaaaagg aatgcgtgga tggaattcga gatgcgctgg ggcgccagat agtcgtcgct
     1141 gattttcagc cagaggccgc cgagcgcgct gccctcgtag ccggtccaat acaaacgcac
     1201 gagttcggca acaataccta tcaagatcaa gccgcaaaga agcgtcagga cgggagcggt
     1261 aaatgcggtc accatcttga tgtcgaaggg aaggccaatc cagatggatt ccgccacggc
     1321 caccaggaag acaaatgccg ccaggacgta gagcaccgca ttggcgcgaa agccgtgaat
     1381 aatgcaggcc gcagtcccgg acagacggtt ctgcaggggc aaggtcagtg cttcactcat
     1441 ggcggcgagg gtcccgttaa aaagccaaac atatcaatcg gaaggttaag gagatgtttc
     1501 gggctggact acagattaga attgttctaa tttgatatca gaggtccata ttgaaaagct
     1561 tctccgatct gtccgaacag gaaatcatcg ccctggccat ttcaagcgag gacgacgacg
     1621 cgcgcgccta ttcgggcttt gccaatgccc tgatggaaac ctacccgggc tccgccaagg
     1681 ttttcatgga aatggcggag gaggagcatc aacaccggcg ctggctgacc gaactttacc
     1741 agaagaaatt cggcgatttc attcctctga tcagacgcag cgagatcaac ggatttgtga
     1801 aacacaagcc ggcctggatg atcggcaagc tcaatatcga agccatgcgc cggcgggccg
     1861 aggtcatcga actggaaaac taccgctttt acaaacaggc ggcggcgcac agccaggacc
     1921 cgcaggtggc caagcttctc aaccgattgg cagaagccga aaagggccac gagtccctgg
     1981 cggcgcgcct tggcgacaaa cacctgactg cggacgtcaa gatcgaggaa ggcgaaacga
     2041 agcgccgcct cttcctgctg caggtcatcc agcccggact tgtcgggctc atggacggct
     2101 cggtctcgac gctcgctccc ttgtttgccg cagccttcgc cacg
//
LOCUS       DAORVN010000382         2142 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_54021_length_2142_cov_3.009104, whole genome shotgun sequence.
ACCESSION   DAORVN010000382 DAORVN010000000
VERSION     DAORVN010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2142
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54021_length_2142_cov_3.009104"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..734
                     /locus_tag="PKV23_07950"
     CDS             <1..734
                     /locus_tag="PKV23_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018182483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQX85405.1"
                     /translation="VVLKVEGLSNPTEFADISFELRRGEILGFYGLVGAGRSEAMQCL
                     FGMTAASSGRITLNGTPLSIASPVDAIAAGISYVPEDRQEQGAVLPLSIRENITLASI
                     GSHVRNFFLSKPSELDAVRVLGRRLDVRAAHWEQRLGDLSGGNQQKVVIAKWLAAKPK
                     VIILDEPTKGIDIGSKAAVHDFIGELAEEGLAVILISSELPEIMGLADRVVVMQEGRI
                     SNIFKRGTWSAETIVSAATGARKAA"
     gene            731..1723
                     /locus_tag="PKV23_07955"
     CDS             731..1723
                     /locus_tag="PKV23_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018182482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQX85406.1"
                     /translation="MSFLLKHRELALSLIIAIMVTGISIYAPAFATFENAASVLNDTS
                     ILCMLALGQMMVILTRGIDLSVASNLALSGMLSASLAQNYPDLPVIAFIAVAIATGLV
                     LGLINGSLVAWLGIPPIVVTLGTLAIFRGMIVVYGGGNQVNASEMGDVFQSFPKVTFF
                     GLSTIFWIMAVVAIAAWLFLSMTRVGRGLYAVGGNPVAARYCGIDLAPQQLLVYSVAG
                     SISGLCGYLWVSRYGVAYSEIASGFELTVIAACVIGGVSIAGGVGTVAGALLGALFLG
                     VIVNALPVMQITPFWQMAISGSVILAAVVVNARAEKRGVKLILPEARLAAGAAK"
     gene            1720..>2142
                     /locus_tag="PKV23_07960"
     CDS             1720..>2142
                     /locus_tag="PKV23_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018182481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQX85407.1"
                     /translation="MSLAPRQDVADAAPKSIADRLLRWESILVVLLIAVIVGNTAISP
                     YFLDIYNLSDATYNFSEKAIVALAMALLILVREIDLSVAAIIALASLAIGLAAQAGAG
                     LPVLLLIGPLAGAACGAFNGFLVTRIALPSIVVTIGTMS"
BASE COUNT          345 a          620 c          678 g          499 t
ORIGIN      
        1 acgtggtttt gaaagttgag gggctctcca atccgactga attcgcggat atttcattcg
       61 agctcaggcg cggcgaaatt ctggggtttt acggcctcgt aggcgccggc cgctcggaag
      121 ccatgcaatg cctcttcggc atgacggccg catcttccgg ccggatcacc ctgaacggaa
      181 cgccattgtc gattgcctca ccggttgacg ccatcgccgc tggcatttcc tatgttccgg
      241 aggaccggca ggagcagggg gccgtgctgc cactcagcat ccgcgaaaac ataacccttg
      301 cctccatcgg ctcgcatgtg cgcaatttct tcctgtcgaa accttcggaa cttgatgcgg
      361 tgcgggtgct tggaaggcgg cttgacgtgc gcgccgccca ctgggaacag cggctcggcg
      421 acctttccgg cggcaaccag cagaaagttg tcattgcgaa gtggctggcc gcaaagccga
      481 aggtcatcat cctcgatgag ccgaccaagg gcattgatat cggctcgaag gccgcggtgc
      541 atgactttat cggcgaactg gcggaggagg ggcttgcggt gatcctgatc agttcggagc
      601 tccctgaaat catgggactg gcggaccgcg tggtggtcat gcaggaggga cggatttcaa
      661 acatcttcaa gcgtggcaca tggtcagccg agacaatcgt atcggcggcg accggcgcac
      721 ggaaggctgc atgagtttcc tgctgaaaca ccgagagctg gcgctttccc tgatcattgc
      781 gatcatggtc accggcatca gcatttacgc cccggctttt gcgacgtttg aaaatgccgc
      841 cagcgttctc aatgacacat caatcctgtg catgctggcg ctggggcaga tgatggtgat
      901 cctgacgcgc ggcattgacc tgtcggttgc ctcaaatctg gcgctgagcg gcatgctctc
      961 ggcatcgctg gcgcagaatt atccggatct gccggtgatc gcctttattg cggtggcgat
     1021 agccacgggc ctggtgctcg gcctgatcaa tggaagtctt gttgcatggc tgggaatccc
     1081 gccaattgtg gtgacgctcg gcacgcttgc catctttcgc ggcatgatcg tggtctatgg
     1141 cggcggcaac caggtgaacg cctcggaaat gggcgatgtt ttccagtcat tccccaaggt
     1201 cacgtttttt ggcctctcca ccattttctg gatcatggcc gtcgttgcca ttgcggcgtg
     1261 gctgttcctg tcgatgacgc gggtaggccg cgggctttac gccgtcggcg gcaatccggt
     1321 tgcggcgcgc tattgcggca ttgatctggc gccgcagcag ctcctggttt attcggtggc
     1381 ggggtcgatc tccggccttt gcggctatct ctgggtttcg cgctacggcg tggcctattc
     1441 ggagattgcc tcggggtttg agctcaccgt cattgcagcg tgtgtcattg gcggcgtgtc
     1501 catcgccggg ggtgttggca cagtggccgg ggctttgctt ggcgcgctgt ttctgggggt
     1561 aatcgtgaat gcgcttccgg tcatgcagat cacgccgttc tggcagatgg cgatctcagg
     1621 cagcgtcatc cttgctgccg tcgttgtcaa tgcgcgggcg gagaagcgcg gcgtgaaact
     1681 catactgccc gaagcgcggc ttgccgctgg agcggcgaaa tgagtctggc tccgcggcag
     1741 gatgtggctg atgccgcgcc caagagtatt gccgaccggt tgctgcgctg ggaaagcatt
     1801 ctggttgtcc tgctgattgc ggtcattgtc ggcaacacgg cgatctctcc ctacttcctc
     1861 gacatctaca acctctctga cgccacttat aatttttccg aaaaagccat tgtggcgctc
     1921 gccatggcgc ttctcatcct ggtccgggaa atcgatcttt cggttgccgc catcatcgcg
     1981 cttgcctcgc ttgccatcgg ccttgcggcg caggccgggg cagggcttcc cgtcctgctg
     2041 ctgatcgggc ctctcgcggg cgccgcctgc ggcgcgttca acggattcct ggtgacgcgc
     2101 atcgcgctgc catcgatcgt ggtgaccatc ggcacgatgt cg
//
LOCUS       DAORVN010000383         2140 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_54122_length_2140_cov_3.036451, whole genome shotgun sequence.
ACCESSION   DAORVN010000383 DAORVN010000000
VERSION     DAORVN010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54122_length_2140_cov_3.036451"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /locus_tag="PKV23_07965"
     CDS             complement(<1..351)
                     /locus_tag="PKV23_07965"
                     /inference="COORDINATES: protein motif:HMM:NF017434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RimK/LysX family protein"
                     /protein_id="HQX85408.1"
                     /translation="MPTLRPTLYPLPLIGWRERVVLPKLGLGPLIAKVDTGARSAALH
                     AEDISIRGSHVRFAIPSNGRKHHYELPIKGQRRVKSTSGHSEMRAVVETEIAIGLVKF
                     TAEITLTNRMDMGVP"
     gene            complement(384..1661)
                     /gene="ribB"
                     /locus_tag="PKV23_07970"
     CDS             complement(384..1661)
                     /gene="ribB"
                     /locus_tag="PKV23_07970"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HQX85409.1"
                     /translation="MKLSEWLANSATSQSELARQLGVTQGRISQIVAGEQPSLDLAMK
                     IAGLTNNRVRPQDFKDMPMSKTLDTVEEAIKAVANGELVVVVDDDDRENEGDLIGAAA
                     KIAPEQMAFMVRHTSGIVCTPMPAEEARRLKLDPMVALNDAPMGTAFTVTIDYKEGLT
                     TGISAKERCATVHALANKNVQAEDFVRPGHIFPLVAKSGGVLMRSGHTEAAVDLARLA
                     GFTPAVGVICELVNDDGTVKRGPQVLTFAREHGLKIISVADLIAYRQLRERLVEQTAK
                     FAVETKIGTAQGYSYVTPFDAVEQLALVYGDISGGKNIPVRLHRENVLEDVFGNRNTL
                     DAVFEIFKREGKGVLVYLQEGTAGVPASQLSGERSASAEARVQTWLDIGLGAQILRDL
                     DVRSIRLLSSSNRHYVGLSGFGIEIAETIKLGE"
     gene            complement(1716..>2140)
                     /locus_tag="PKV23_07975"
     CDS             complement(1716..>2140)
                     /locus_tag="PKV23_07975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85410.1"
                     /translation="IADDSLLIHELAGAKLAQFFPGTWGPQGLFVGELIYFATTGAFL
                     ALIFAYSYVQSERKHRARSVLLLLPFGMLAFFAVLFDYFRESIRGISRLADFFVGMIE
                     DGSELLCMSIIMLLAIGLVIASSGETVRQRPVGDQVGA"
BASE COUNT          425 a          683 c          612 g          420 t
ORIGIN      
        1 gggaaccccc atgtccatgc ggtttgtgag agtgatttca gcagtgaatt tgaccaggcc
       61 gatggcgatc tcggtttcca ccacggcgcg catttcgctg tgcccgctgg tgcttttcac
      121 ccgccgctgg cccttgatcg gcaattcata gtgatgcttc cggccattgc tgggaatggc
      181 aaagcgcaca tggctgcccc tgatgctgat gtcttccgca tgaagcgccg cggacctcgc
      241 ccccgtatca accttcgcaa tcagcggccc cagtccgagc ttcggcaaaa caacccgctc
      301 gcgccagccg atcagcggaa ggggataaag cgtggggcgc agggtgggca tggggatttg
      361 ttagcggaat caaaccctgt tcgctactcc cccagtttga tcgtctccgc gatctcgatc
      421 ccgaagccgg aaaggcccac ataatgccgg ttgctcgatg agaggagccg gatcgagcgc
      481 acgtcgaggt cgcgcaggat ctgcgccccc aagccgatat ccagccaggt ctgcacccgc
      541 gcctcggcac tggccgaccg ctcgccggaa agctggctcg ccggaacgcc ggcggttcct
      601 tcctgaagat aaaccagcac gcccttgccc tcgcgcttga aaatctcgaa caccgcgtcc
      661 agcgtgttgc ggttgccgaa cacgtcttcc agcacattct cccggtgcag gcgcacgggg
      721 atgttcttgc cgccggaaat gtcaccgtaa accagcgcca gctgttccac cgcatcaaat
      781 ggtgtcacat aggaatagcc ctgcgccgtg ccgattttcg tctccaccgc gaacttcgcc
      841 gtctgctcca ccaggcgttc gcgcaattgc cggtaggcga tgaggtcggc caccgaaatg
      901 atcttcaggc catgctcgcg cgcaaaggtc agaacctgcg gcccccgctt taccgtgccg
      961 tcatcattca ccagttcgca gatcacgccg actgccggcg tgaagcccgc cagccgcgcc
     1021 aggtcaacgg ctgcttccgt atggcccgag cgcatgagaa ccccgccgga tttggccacc
     1081 agcggaaaaa tgtggccggg ccgcacgaaa tcctccgcct ggacgttctt gttggccagc
     1141 gcatgcaccg tggcgcaacg ctccttggcc gaaatgcccg tggtcaatcc ttccttgtag
     1201 tcaatggtga cggtgaaggc cgtgcccatc ggcgcatcgt tgagcgccac catgggatca
     1261 agcttcagcc gccgcgcctc ttcggccggc atgggggtgc agacgatgcc cgaggtgtgg
     1321 cgcaccatga aggccatctg ctccggcgcg atcttggcgg cggcgccgat caggtcgccc
     1381 tcgttttccc ggtcgtcatc gtcaaccacc accaccagtt cgccattggc caccgctttg
     1441 attgcttctt ccaccgtgtc gagtgtcttg ctcatgggca tgtccttgaa atcctggggg
     1501 cgaacccggt tgttggtaag gccggcgatt ttcatcgcca gatcgagcga aggctgttcc
     1561 cccgccacaa tctgcgaaat gcgcccctgg gtgaccccca gttgccgcgc caattccgat
     1621 tgcgaggtgg cgctattagc cagccattcc gaaagtttca tggagaatta ttagcagagc
     1681 taatagttag agtcgataac tctgttcaga tgcatctatg cgcccacctg gtcgccgacc
     1741 ggtctctgcc ggaccgtttc gccactgctg gcgatgacca ggccgatggc tagcagcatg
     1801 atgatggaca tgcacaggag ttccgatccg tcctcgatca tgccgacaaa aaagtctgcc
     1861 aggcgggaaa tgccacggat cgattcacga aagtaatcga agaggaccgc aaagaatgcc
     1921 agcattccga aaggaagcag aagcaagacg cttctcgccc ggtgtttccg ttcggattgg
     1981 acgtatgaat aggcgaaaat cagggccaga aaagccccgg tcgtggcaaa atagataagc
     2041 tccccgacaa acagcccctg cggcccccat gtgccgggaa agaattgagc cagcttcgcg
     2101 cctgccagct catgaatgag caggctgtcg tcggcaatcg
//
LOCUS       DAORVN010000384         2137 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_54255_length_2137_cov_2.655139, whole genome shotgun sequence.
ACCESSION   DAORVN010000384 DAORVN010000000
VERSION     DAORVN010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2137)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2137
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54255_length_2137_cov_2.655139"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..944
                     /locus_tag="PKV23_07980"
     CDS             <1..944
                     /locus_tag="PKV23_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012495740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQX85411.1"
                     /translation="KSFGERAIVSGLDLLMLRGDRLGIVGPNGAGKTTLIKMLTGAME
                     PDSGSVRLGTNQQVVTLDQSRQSLDPNTLLADVITAGRGQSVTINGETRHVASYMKDF
                     LFRPEQARTPVSVLSGGERARLILARELAKPSNFLVLDEPTNDLDLETLDLLQEMLGD
                     YQGTILLVSHDRDFLDRVATSILMFEGDGKWTEYAGGYSDMVAQRGAGVNARALKVAV
                     SERAASSAQPSKPQARRKLTFKDKHALETLPAKMASLHDEIVALEAKLADPELFARDP
                     KGFEKAMSRHQKAKAEMTQAEEQWLELEMLKEELEAN"
     gene            1074..1820
                     /locus_tag="PKV23_07985"
     CDS             1074..1820
                     /locus_tag="PKV23_07985"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQX85412.1"
                     /translation="MKRSSLLSVFGLAIVMLSDPSIAETFEDGHAAYDSGDYAAAFQI
                     WKSLADQGSPSAQYGLGEMYSLGLGVSRDDPEAAKWFGMAASSDHAPAQSRLGAMYEV
                     GRGVPQSLSKAMKWYRKAAQQGDLPAQVSLGLIYGTGRGVPQNYFQAVKWFRLASEQG
                     DALAQFNLGLIYASGNDVVPEDDLEALVWFILAAKGGNQEARKNQEAFAKRMSPEQVK
                     RAEKLAQDWMTTKAEDKAAKCSMSGAIECK"
     gene            complement(1876..>2137)
                     /locus_tag="PKV23_07990"
     CDS             complement(1876..>2137)
                     /locus_tag="PKV23_07990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85413.1"
                     /translation="NEWNAKFTAVLAAAALAGAAAPVWYSGSNWNSGNMGGFTESLGS
                     GLAASAASASRAPGSGSSSGGGFSGGGGSSGGGGGGGGGGGW"
BASE COUNT          475 a          609 c          653 g          400 t
ORIGIN      
        1 ccaaatcctt tggcgagcgg gcaattgtct caggccttga cctgctgatg ctccgtggtg
       61 acaggcttgg aatcgtgggg ccgaatgggg ccggcaaaac cacgctgatc aaaatgctta
      121 ccggcgccat ggaacccgac agcgggtcgg tgcggcttgg caccaaccag caagttgtga
      181 cgctggatca aagccgccaa agccttgatc ccaataccct gctggctgac gtgatcacgg
      241 ccggacgcgg ccaatcggtg accatcaatg gcgagacccg ccatgtggcg agctacatga
      301 aggacttcct gttccggccg gaacaggcgc ggacgccagt ttcagtgctg tccggcggcg
      361 agcgggcgcg gcttatcctg gcacgcgaac tggccaagcc ctcgaatttc ctggtgctgg
      421 acgagcctac caatgacctc gatctggaga cgctcgatct cctgcaggaa atgctgggtg
      481 actaccaggg aacgattttg ctggttagcc atgaccgcga cttcctcgac cgggtggcca
      541 cctccatcct gatgttcgag ggtgatggaa aatggacgga atatgccggc ggttattcgg
      601 acatggtggc gcagcgtggc gccggcgtga atgcacgcgc attgaaagtt gccgtgagtg
      661 aacgggccgc ttcatctgca cagccgtcta aaccgcaggc ccggcgcaag ctgacattca
      721 aggacaagca tgcgcttgaa actctacctg ccaagatggc gtccctgcat gacgagatag
      781 ttgcgcttga agcaaaacta gccgacccgg agcttttcgc ccgggatccc aagggctttg
      841 aaaaggccat gtcgcgccac cagaaagcca aggcggaaat gacccaggcc gaggagcagt
      901 ggcttgaact tgaaatgctg aaggaagaac tggaagcgaa ctaattcgag cgtttgccac
      961 acgcgagaag tgcatcgcgg gtccaatttg gcttgctact ctgtttgatc tatacttcgt
     1021 taccctggaa gaaaggcttg gccggccctg gcaggactct ggtcgtgaat gagatgaaac
     1081 gcagttccct gttatcagta ttcggactag ccattgtcat gctgtccgat ccatcgattg
     1141 cagaaacttt cgaggacggt catgccgcct atgacagcgg cgactatgcc gcagcatttc
     1201 agatctggaa gagcctggcc gatcaaggca gcccatcggc gcagtatggc cttggcgaga
     1261 tgtattccct gggccttggt gtgtctcgag atgatccgga agcggccaaa tggtttggga
     1321 tggcggcctc gagcgaccat gcgcctgccc aatcgcggct gggtgcgatg tatgaagtgg
     1381 gccgcggcgt cccgcaaagc ctgtccaaag cgatgaaatg gtaccggaaa gctgcgcagc
     1441 agggtgacct gccagcccag gtttcgcttg ggctcatcta cggcactggc cgcggtgttc
     1501 cgcagaacta ctttcaggcg gtaaaatggt tccggcttgc gtctgaacaa ggcgatgcgc
     1561 tggcgcaatt caatcttggg cttatttatg ccagtggcaa tgatgttgtg ccggaagacg
     1621 atttggaggc tctggtttgg ttcatccttg ctgcgaaggg aggcaatcag gaggcgcgca
     1681 agaaccagga agcctttgcc aaacgaatga gtccggaaca ggtgaaaagg gcagaaaagc
     1741 tggcacagga ctggatgacc acgaaagcag aagacaaggc agccaaatgc tccatgtctg
     1801 gggcgatcga atgcaagtga acaacaaaaa tgcgggcaaa agcgctttca gctgctgcag
     1861 attcacgccc taggcttacc agcctccgcc gccaccgccg ccgccgccgc cacccgacga
     1921 cccgcctcca ccggagaagc cgccgccgga acttgagcca gaccccgggg cccgcgaagc
     1981 tgaggccgca cttgcagcaa ggcccgaacc caggctttcg gtgaagccgc ccatgttacc
     2041 ggaattccag ttgctcccgg aataccagac aggggccgcc gcacccgcca gggcagcggc
     2101 ggcgagcaca gccgtgaact tggcattcca ctcattc
//
LOCUS       DAORVN010000385         2134 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_54380_length_2134_cov_5.092833, whole genome shotgun sequence.
ACCESSION   DAORVN010000385 DAORVN010000000
VERSION     DAORVN010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54380_length_2134_cov_5.092833"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..904)
                     /locus_tag="PKV23_07995"
     CDS             complement(<1..904)
                     /locus_tag="PKV23_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003526569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase family protein"
                     /protein_id="HQX85414.1"
                     /translation="MNVNATPRDYGLLGRDSRIAVEMGLVSAQWYQCPMPRKRLKELM
                     QRRDGPAIRDSLIWFASFLSSGGLAFYFWPTWAALPFFLIYGVLYGSSSDSRWHECGH
                     GTAFKTRWMNDALYHIACFMIMREPTVWRWSHTRHHTDTIIVGRDPEIAVMRPVMILK
                     IIAAFLAIPQTWNSVKSLVRHALGRIGAEEATFIPEMERGKVYMVARIWLAIHAAVIA
                     FAVYSGSILPLLFVGPLPTIYGAWLHVVTGLTQHGGLAENVLDHRLNSRTVHMNPVLR
                     FMYWNMNYHIEHHMFPMVPYHALPE"
     gene            1037..2065
                     /locus_tag="PKV23_08000"
     CDS             1037..2065
                     /locus_tag="PKV23_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018183604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HQX85415.1"
                     /translation="MGKRATITDLAREAGVSVATVDRVLNQRLPVREDTALRVVAAAE
                     SIGYHATGLLKQRLTEVRPCTMGFLLQKRADDFYQALANELLNATKATPSIRGKAVIE
                     FMDELVPAFIASKIRDIGARVDALAIVAVDHPLVSEAVEEIVARGKPVFTLLSDLTTP
                     ARAGYVGVDARKAGRTAAWTISRLARKPGKIGILLGSHRYLSQETAEISFRSYMRENA
                     PQFQVLEPLVNLDDQRIAYEAVEDMISSNADLVGIYMAGGGMAGLVRALRDEKAGDRI
                     IAICNELIPITRSALIDGTIDMVLATPVATLSSHAVAAMAQSVVSGRYEGGILVQLPA
                     EIFVSENI"
BASE COUNT          468 a          606 c          649 g          411 t
ORIGIN      
        1 gctcgggaag cgcgtgatag ggcaccatgg ggaacatgtg atgctcgatg tggtagttca
       61 tgttccagta catgaaccgc agaaccggat tcatgtggac cgtgcggctg ttgaggcggt
      121 ggtccaacac attctccgcc aggccgccgt gctgcgtcag gcccgtgacg acatggagcc
      181 aagccccata tatggttggc aagggtccca cgaacagcag gggcagaatg gatcccgaat
      241 aaaccgcgaa ggcgatgacg gcggcgtgga ttgccagcca gatgcgggcc accatgtaga
      301 ccttgccccg ctccatctcc ggaataaacg ttgcctcctc ggcgccgatt cttcccaggg
      361 cgtggcggac caggctcttg accgaattcc aggtctgcgg gatggcaagg aacgcggcga
      421 tgatcttgag gatcatcacc gggcgcatga cggcgatttc cggatcgcgt cccacgatga
      481 tggtgtctgt gtgatggcgc gtatggctcc agcgccagac cgtgggttcg cgcatgatca
      541 tgaagcaggc gatgtgatag agggcgtcat tcatccagcg ggtcttgaag gccgtgccat
      601 ggccgcactc atgccaacgc gaatccgagg acgagccata gagcacgccg taaatgagaa
      661 agaacggcag tgccgcccat gtgggccaga aataaaaggc aagcccgccg ctggagagga
      721 acgacgcaaa ccagatgagg ctgtcgcgga tcgccggccc atcgcggcgc tgcatcagct
      781 ctttaagccg cttgcgcggc atcgggcatt gataccactg ggccgacact aggcccattt
      841 ctacggcaat cctggagtcc cggccgagaa ggccataatc gcgtggcgtg gcattcacat
      901 tcatgggcaa ctactccgaa atgtgctggt tccatagtcg aggaaaccat agggggcaaa
      961 cagattgtga tatattctat caaattgcat cagaaacttt catgtgctag ggagtgaagc
     1021 gctgaaggaa accaggatgg gaaaaagggc aacaattacc gatctggcgc gtgaggccgg
     1081 cgtgagcgtc gccaccgtcg accgtgtcct gaaccagcgc ctgccggtcc gcgaggatac
     1141 ggcccttcgc gtagtggccg ccgccgaatc catcggctat cacgccaccg gcctgctcaa
     1201 gcaacgcttg actgaagtca ggccctgcac catggggttc ctgctgcaga agcgcgccga
     1261 tgatttttac caggcgctgg caaatgaact cctgaatgcc accaaggcga caccttccat
     1321 tcgcggcaaa gctgtcatcg agttcatgga cgaacttgta cctgccttca ttgcctcaaa
     1381 gatccgcgac atcggcgcgc gggtcgatgc cctggcgatt gtcgccgtcg accatccgct
     1441 ggtaagcgaa gctgttgagg aaattgttgc gaggggaaag cccgtattca ccctgctgag
     1501 cgatctcacc accccggcga gggccggata cgttggcgtg gatgcgagaa aagccggccg
     1561 taccgctgcc tggaccatat ctcgcctggc caggaagcct ggaaaaattg gcattctcct
     1621 cggcagtcac cgttacctca gccaggaaac ggccgagatc agcttcagga gctacatgcg
     1681 cgagaatgcg ccccagttcc aggtgctgga gccgcttgtg aatctggacg accagcgcat
     1741 cgcctatgag gctgtcgaag acatgatttc gagcaatgca gacctagttg gaatttatat
     1801 ggcgggcggc ggcatggctg ggctggtgcg ggcactgcgc gatgagaagg ccggggaccg
     1861 gatcatcgcc atatgcaacg aattgatccc gatcactcgc agcgccctga tcgacggaac
     1921 cattgatatg gttctggcta cgcctgttgc gacactgtct tcccacgccg ttgcggccat
     1981 ggcccaatcg gtggtttccg gccgttacga gggcggcatt ctggttcagc ttcccgccga
     2041 gatatttgtc agcgaaaaca tctgaaggtc cctatcaacc aaggcctcca ttggaatgat
     2101 agattttgat aggttcgcat gcaaccccaa gagg
//
LOCUS       DAORVN010000386         2127 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_54732_length_2127_cov_2.704151, whole genome shotgun sequence.
ACCESSION   DAORVN010000386 DAORVN010000000
VERSION     DAORVN010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54732_length_2127_cov_2.704151"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..134
                     /locus_tag="PKV23_08005"
     CDS             <1..134
                     /locus_tag="PKV23_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003542906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HQX85416.1"
                     /translation="DRGVVVPDGLVVGEDPVADAQRFRRTENGVCLITKPMIAKLPA"
     gene            131..1564
                     /gene="glgA"
                     /locus_tag="PKV23_08010"
     CDS             131..1564
                     /gene="glgA"
                     /locus_tag="PKV23_08010"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008839400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HQX85417.1"
                     /translation="MKVLSVASEVFPLVKTGGLADVAGALPAALAKQGMNVITLMPGY
                     PAVMTALENYETVFSTDGHFGGAARLLWGKAKGLDLFVLDAPHLYDRPGNPYLGPDGK
                     DWPDNSRRFAALATIAAEIGRGLVADFVPEIIHCHDWQAGLVPAYVRYGGGPKTIMTV
                     HNLAFQGHYPASIFGTLGLPASAFGIDGVEYFGGVGYLKAGLQCSDAITTVSPTYARE
                     IMTVEDGMGLDGLLRARSQAVHGIVNGIDTEIWNPADDAHIAQTYTAKHLERRDANKR
                     AIERRFGLESGGGLLICIVSRLTLQKGMDLVAGALPTLMQLGGRLAILGSGDAALEGA
                     LKSAMVNYPGRVGIATAYDEPLSHLLQAGSDAILIPSRFEPCGLTQLYGLRYGCVPIV
                     ARVGGLADTVVDANDAALSAHAATGIQFSPVTQEALEHALRRTADLYANRRAWASLQQ
                     AGMKSDVSWTRSAARYAELYRALLKGA"
     gene            1566..>2127
                     /locus_tag="PKV23_08015"
     CDS             1566..>2127
                     /locus_tag="PKV23_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008873442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HQX85418.1"
                     /translation="MIKTIATTPFEGQKPGTSGLRKKVAVFQQGHYTENFIQSIFDSL
                     QGFTGKTLVIGGDGRFHNRDVIQTAIRMAAANGFGRVLTAKGGLLSTPAASHLIRMRA
                     AFGGIILSASHNPGGPDGDFGIKYNAENGGPAPEKLTDAFFANTQKISSYKISDGADC
                     DLDKLGMSTVEGMTVEVIDPVSDYQKL"
BASE COUNT          401 a          611 c          692 g          423 t
ORIGIN      
        1 ttgaccgcgg cgtcgtggtt ccagacggac tggttgtggg cgaggatccg gtggccgacg
       61 cccagcgctt caggcgtacc gaaaatggcg tctgcctcat caccaagccg atgattgcaa
      121 aactgccggc atgaaagtcc tgagcgtcgc atcggaagtg ttcccgctgg tcaagaccgg
      181 cgggctggcc gatgtcgccg gcgcgctgcc ggcggcgctg gccaagcagg gtatgaacgt
      241 cattacgctg atgccgggct atccggcggt gatgacggcg ctggagaatt atgaaaccgt
      301 cttttccacc gacggccact ttggcggggc ggcgcggctg ctgtggggca aggccaaggg
      361 attggacctc ttcgtgcttg atgcgccgca tctctacgac cggcctggaa atccctatct
      421 cggcccggac ggcaaggact ggcccgacaa ttcccggcgc tttgcggcgc tggcaacgat
      481 tgcggcggag atcgggcggg gcctggtggc ggattttgtc cctgagatca ttcactgcca
      541 cgactggcag gcgggacttg ttccggccta tgtgcgctat ggtggcgggc ccaaaaccat
      601 catgacggtt cacaacctgg cctttcaggg acattacccg gcttccatct tcggcactct
      661 tgggcttccg gcctcggctt tcggcattga cggggtggag tatttcggcg gggtcggcta
      721 cctgaaagcg ggactgcaat gttccgatgc catcaccacg gtgagcccca cctacgcacg
      781 cgaaatcatg acggttgagg acggcatggg gcttgacggg ttgctgcggg cgcggagcca
      841 ggcggtgcat ggcattgtca acggcatcga tacggagatc tggaatcccg ccgatgatgc
      901 acatatcgcg cagacctata cggcaaagca cctggaacgg cgcgatgcca acaaacgggc
      961 cattgaaagg cgtttcggtt tggaaagcgg cggcggactg ctcatctgca tcgtcagccg
     1021 gcttacgctg cagaagggca tggacctggt ggccggcgcc ctgccaacgc tgatgcagct
     1081 cggcggcagg cttgcgatcc tggggtcggg ggatgcggcg ctggagggcg cgctcaagtc
     1141 ggccatggtg aattatccgg gccgcgtcgg cattgccacg gcctatgacg agccgctttc
     1201 gcatctcctg caggcgggtt cagatgccat tctcatcccg tcgcgtttcg agccatgcgg
     1261 cctcacgcag ctttatggct tgcgctatgg ctgcgtgccc attgtggcgc gggtcggcgg
     1321 gctggcggac acggtggtcg atgccaatga cgcggcgctc tcagcccatg ccgcaacggg
     1381 catccagttt tctccggtga cgcaagaggc gctggaacat gccttgcggc gcacggcaga
     1441 tctttatgcc aacaggcgcg cctgggcctc tctgcagcag gctgggatga aatccgatgt
     1501 ttcgtggaca cgcagtgcag cgcgctatgc ggaactttac cgcgctctgc tgaaaggcgc
     1561 ctgagatgat caagacaatt gcgaccacgc catttgaagg acagaagccc ggcacatcgg
     1621 ggttgcgcaa aaaagtggca gtgttccagc aggggcatta tacggagaat tttatccagt
     1681 ccattttcga ttcattgcaa gggtttacgg ggaaaacctt agttattggt ggagatggac
     1741 ggttccacaa ccgggacgtc attcagactg ccatccgcat ggcggcggcc aatggtttcg
     1801 gccgggtgct gacggccaaa ggcgggctgc tttcgacgcc ggccgcctcg catcttatcc
     1861 gcatgcgggc ggccttcggc ggcatcatcc tttcggccag ccacaatccc ggaggaccgg
     1921 acggcgattt cggcatcaag tacaatgcgg aaaatggcgg ccctgctccg gagaaactga
     1981 cggacgcgtt tttcgccaac acacagaaaa tctccagcta taaaatttcc gatggcgctg
     2041 actgcgatct cgacaaactg gggatgagca cagtcgaggg catgacggtt gaggtcatcg
     2101 atccggtttc cgattaccaa aagctca
//
LOCUS       DAORVN010000387         2126 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_54767_length_2126_cov_3.701593, whole genome shotgun sequence.
ACCESSION   DAORVN010000387 DAORVN010000000
VERSION     DAORVN010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2126)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2126
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54767_length_2126_cov_3.701593"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..805
                     /locus_tag="PKV23_08020"
     CDS             <1..805
                     /locus_tag="PKV23_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010658722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HQX85419.1"
                     /translation="SVAPSTTKNEPTVVLLTPGIHNSAFFEHAFLADEMGVELCEGDD
                     LFVSDGWLYMRTTQKPRRVDVVYRRIDDAYLDPLAFRPDSNLGVPGIFDVYRAGHVTL
                     VNAPGTGVADDKSIYTYMPEIIRFYTGEKPLLNNVPTWRCSSENDLKYVLEHLAELVV
                     KEVHGSGGYGMLVGPTSSKAEIEQFRAKVMARPTNYIAQPTLALSAVPTFTNSGIAAR
                     HVDLRPFVLSGDQIRITPGGLTRVALKEGSLVVNSSQGGGTKDTWVLED"
     gene            808..1755
                     /locus_tag="PKV23_08025"
     CDS             808..1755
                     /locus_tag="PKV23_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014129606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HQX85420.1"
                     /translation="MLGRTASSLYWMSRYVERAENMARLLEVGYRISLMPGAIEGHRD
                     EWRSTLTSAACEQSYTEKHGDIDGAKVIHFLLFDESNSSSVKSCLKTARNNGRGVRTA
                     LTRDVWESLNSTWNELAHIQPENITSDRLPEFLEWIKQHAMGFRGALLGTMLRTDNYY
                     FSQLGNFIERADNTARILDVKYFILLPRASDVGSDLDMQQWAQILRSVSAHRAYRWFY
                     KDSRYKPWLVSEFLILKEQMPRSLLFTYNWINLALDGLGELYGNRYACHELARETHRR
                     LKNGDMEEIFQEGLHEFLQEFMAANNRLSQSIATTYNFP"
     gene            1760..>2126
                     /locus_tag="PKV23_08030"
     CDS             1760..>2126
                     /locus_tag="PKV23_08030"
                     /inference="COORDINATES: protein motif:HMM:NF019977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase N-terminal domain-containing
                     protein"
                     /protein_id="HQX85421.1"
                     /translation="MRIHIRHETFYAYETPLSYSVQRLYLTPQNFAAQKIADWKITAP
                     GIEGALTYLDGFGNRIHLVTIQNIEGPMSIVAEGLVDVEDAAGVVKGLRCPAPDAVFL
                     RQTAATRPSPAILAMIEKVD"
BASE COUNT          433 a          634 c          654 g          405 t
ORIGIN      
        1 cagcgtggcg ccatccacca cgaagaacga acccaccgtg gtgctgctga cgcccggcat
       61 ccacaactcg gcgtttttcg agcacgcctt tctggcggac gagatggggg ttgaactgtg
      121 cgagggcgat gatctttttg taagcgatgg ctggctttac atgcgcacga cgcagaagcc
      181 gcggcgggtg gatgtggtct accggcgcat cgatgatgcc tatctcgacc ccctcgcctt
      241 ccgcccggat tccaacctcg gggttccggg gattttcgat gtttaccggg cgggccatgt
      301 gaccctggtg aatgcgccgg gcacaggcgt ggccgacgac aagtcgatct acacctacat
      361 gcccgaaatc atccggtttt atactggcga gaaaccgctg ctcaacaatg tgccgacctg
      421 gcgctgctcg agtgagaacg atctcaaata tgtgcttgag catctggcag aactggtggt
      481 gaaggaggtt catggctccg gcggctatgg catgctggtg gggcccacct catccaaagc
      541 cgagattgaa cagttccgcg ccaaggtcat ggcgcggccc acgaactata ttgcccagcc
      601 aaccctggcg ctctcggcgg ttcccacctt tacgaactcg ggcatagcgg cccggcatgt
      661 tgacctgcgg ccctttgttc tgtcggggga ccagatccgc atcacgccgg gcgggctcac
      721 gcgggtggcg ctcaaggaag gctcgctggt ggtcaactcg agccagggcg gcggcaccaa
      781 agacacctgg gttctggagg actagggatg ctgggacgca ccgcctcatc gctctactgg
      841 atgtcacgtt atgtggagcg ggcggagaac atggcgcggc ttctggaagt gggctaccgc
      901 atttcgctga tgcccggggc catcgaaggg caccgggacg aatggcgctc gacgctcacc
      961 agtgcggcct gcgaacagag ctacacggaa aagcatgggg acatcgacgg ggcgaaggtc
     1021 atccatttcc tgctgtttga cgagagcaac tcctcgagcg tgaaatcctg cctgaagacg
     1081 gcgcgcaaca atggccgggg ggtgcgcacg gcgctgaccc gcgacgtgtg ggaaagcctc
     1141 aactccacct ggaacgagct ggcccatatc cagccggaga acatcacctc cgaccggctt
     1201 ccggaattcc tcgaatggat caagcagcac gccatgggct ttcgaggtgc gctgctcggc
     1261 accatgctgc gcaccgacaa ctactatttc agccagctcg gcaatttcat cgagcgggcc
     1321 gacaacacgg cgcgcatcct cgacgtgaaa tacttcattc tcctgccgcg ggccagtgac
     1381 gtgggcagcg atctcgacat gcagcagtgg gcgcagattc tgcgttcggt atccgcccac
     1441 cgggcctatc gctggttcta caaggacagc cgttacaagc cgtggctggt ttcggaattt
     1501 ctcatcctca aggagcagat gccgcgctcc ctgctcttca cctataactg gataaacctg
     1561 gcgctggacg gattgggcga actctatggc aaccgctatg cctgccacga gcttgccagg
     1621 gaaacccatc gccggctgaa gaacggcgac atggaggaga ttttccagga gggcttgcat
     1681 gaattcctgc aggagttcat ggcggccaac aaccgcctgt cgcaaagcat agcaacaacc
     1741 tataattttc cgtgacctga tgcgcattca catccgccat gaaacattct acgcctatga
     1801 aacgccgctg agctattcgg tgcagcggct ctatctgacg ccgcagaatt tcgcggcgca
     1861 gaagattgcc gactggaaaa tcacggcgcc tggaattgaa ggggcgctta cctatctcga
     1921 cggcttcggc aaccgcattc atctcgtgac catccaaaat atcgaaggcc cgatgagcat
     1981 cgtggcggaa ggcctggtgg acgtggaaga tgcggcaggc gtggtgaagg gcctgcgctg
     2041 cccggcgccc gatgcggtgt tcctgcggca gaccgcggcg acgcggccga gcccggccat
     2101 tctggcgatg atcgaaaagg tcgatg
//
LOCUS       DAORVN010000388         2123 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_54911_length_2123_cov_3.707447, whole genome shotgun sequence.
ACCESSION   DAORVN010000388 DAORVN010000000
VERSION     DAORVN010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2123
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54911_length_2123_cov_3.707447"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..729)
                     /locus_tag="PKV23_08035"
     CDS             complement(<1..729)
                     /locus_tag="PKV23_08035"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQX85422.1"
                     /translation="MLRSLSRPDVLVVTLAAAGIMMVTMGSRQSLGLFIGPIEATTGM
                     GIASISLALAIGQFTWGAIQPVAGAVADRYGARAVLIGGLLILALGSAITPFVTSGFG
                     LMISLGLLSAMGSGIGSFSVLIGAAAQRLPLESRGTASGVINAGGSFGQFVFAPILQK
                     LIQGVGWMGAMWAMALMTLAALPLVGRLTRPVEGQVTHVHEEGGLKRAVGKAMTDRSY
                     LLLHAGFFTCGFHIAFLVTHLPGEV"
     gene            832..1758
                     /locus_tag="PKV23_08040"
     CDS             832..1758
                     /locus_tag="PKV23_08040"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HQX85423.1"
                     /translation="MQWLNSWESFVKVVETGSMAAAARRLDCTRAQISKQIGDLERAF
                     GVRLFERSTRKISLTPSGEIFHQHALRALEAIHSTEVAVRNMGDAPHGVLRISASITF
                     GRMYIAPLLPIIVAKYPDLNCELVLTDHLVDLVEENIDLALRLTKTPPEDAVARKLVH
                     MKRVICATPGYFAAHGEPRTPHELAHHACFSLLQLNESRIWRLIDAQGEEVSVPVTSK
                     FLFNDIDCVMNAVLAGHGIGMLPTCLCGPALARGELRKIFDDLEPLSSLGRYLYACYT
                     PNRVRVPKVRVFLDELEKLFNPQPPWERPEAA"
BASE COUNT          503 a          649 c          589 g          382 t
ORIGIN      
        1 cacctcgccc ggcagatggg tgacgagaaa ggcaatgtgg aaaccgcagg tgaagaaacc
       61 ggcatgcagc aaaagatagc tgcggtccgt catggccttg ccgaccgccc gcttcagccc
      121 gccctcttcg tgcacatgcg tgacctgccc ctcgaccggc ctggtcagtc tgccaaccag
      181 cggcaaggcc gccagcgtca tcaatgccat ggcccacatc gcccccatcc agcccacgcc
      241 ctggatcagc ttttgcagaa tgggcgcaaa aacgaactgg ccgaaagagc cgccggcgtt
      301 gatcaccccg gaggccgtac cgcgggactc cagcggcagg cgctgggcgg cggcgccgat
      361 cagtaccgaa aaactgccta tcccggaacc catggccgag agcaaaccga gcgaaatcat
      421 cagcccgaaa ccgctagtca cgaacggtgt aatcgccgaa cccagcgcca gaatgagtaa
      481 gccgccaatc agcacggcac gcgcgccgta gcgatcggcg acagcgccgg ccaccggctg
      541 aatggcaccc caggtgaact gcccgatggc cagggccagg ctgatgctgg caatgcccat
      601 gccggtcgtt gcctcgattg gcccgatgaa cagccctagc gactggcggg aacccatcgt
      661 aaccatcatg atgccggcgg cggccagcgt aacgaccaga acatcggggc gactcaggga
      721 gcgcaacatg gctatttcct gcggtttgtt attgtggtga aactatacct agcaaaatca
      781 ttggcaaaaa gccgataatt atcgccataa cgtcaacgga tcattgacaa aatgcaatgg
      841 ctcaatagct gggaaagctt cgtcaaagtg gtggaaacgg gcagtatggc tgctgcggcg
      901 cgtcggctgg actgtacccg ggcgcagatc agcaagcaaa tcggcgatct tgagcgcgcc
      961 ttcggcgtcc gcctctttga acgatccacc cgcaagatca gcctgacgcc ctctggcgag
     1021 atttttcacc agcacgccct gcgcgcgctg gaggcgatcc acagcaccga agttgcggtc
     1081 agaaacatgg gcgatgcgcc gcacggcgtt ctgcgcatca gtgcctcgat caccttcggg
     1141 cgcatgtata tcgcgccgct gctgccgatc attgttgcca aataccccga cctgaattgc
     1201 gaactggtcc taaccgatca tctggtcgat ctggtcgaag aaaacatcga cctagcgctg
     1261 cgcctgacca agacgccgcc ggaggatgcc gtcgcccgca agctagttca tatgaagcgc
     1321 gttatttgcg cgacaccagg ctattttgcc gcccacggtg aacctcgaac accgcacgaa
     1381 ctggcacacc acgcctgctt cagtttgttg cagctcaacg aaagccgaat atggcgactg
     1441 atcgacgccc aaggagagga agtcagcgtg ccggtcacga gcaaattcct gttcaacgac
     1501 atcgattgcg tcatgaatgc cgtattggct gggcatggca ttggcatgtt gccgacctgc
     1561 ctgtgtggcc cggcactggc ccgcggtgaa cttcgcaaga tcttcgacga tctcgagccg
     1621 ctcagtagcc tcggccgtta tctctacgcc tgctacacgc caaaccgggt ccgcgttccg
     1681 aaagtccggg tctttcttga cgaactggaa aagctgttca atccccagcc cccgtgggag
     1741 cggccagagg ctgcctagta ccaaaaggtg cattgcagaa tatcacctga cttgaccgcc
     1801 atttcgcaca aagattaatc ggtgaagatc acctacccaa tcagttcagg agattggttt
     1861 tgcgatgggt atatggccgt actcggccag aagcggacac tgggttaccc cgccccaaag
     1921 cagacattgc cctgcgtctg tgtaagtatc gttagccata cccatccaac tcgccgaggg
     1981 ctgcaaaatg gaagaacagg cagtgagcaa atcgcttgcg aaagaagtgt cggaattcct
     2041 gatgcaagga atcgaactcg agggacgaac tctcgaacac agtatttcaa ttcgcgaaca
     2101 atcgaaggcc aaatataaac aag
//
LOCUS       DAORVN010000389         2122 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_54963_length_2122_cov_3.707789, whole genome shotgun sequence.
ACCESSION   DAORVN010000389 DAORVN010000000
VERSION     DAORVN010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2122)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2122)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2122
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54963_length_2122_cov_3.707789"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(24..554)
                     /locus_tag="PKV23_08045"
     CDS             complement(24..554)
                     /locus_tag="PKV23_08045"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HQX85424.1"
                     /translation="MTIETKQANWVPQLAALNVAALIDHIVSHYHEIHRQELPELLAL
                     ARKVERVHHDVPEAPLGLADVLERLSDELITHMRKEEFVLFPAMRQGVNEGLAQPITM
                     MRHEHEDHAGTICRLEELARGFAVPEGACGSWQRLYAGLKKLCEDIRAHIRVENDVLF
                     PRFELTAKSGCICAHG"
     gene            750..1157
                     /locus_tag="PKV23_08050"
     CDS             750..1157
                     /locus_tag="PKV23_08050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HQX85425.1"
                     /translation="MRLAAEPERVFTTDEIANEFAISRNHLMKIIHNLAKAGIVVTLR
                     GVGGGFRLSKSPEEIPLGGLVRLLEQRHALVECFQADGGQCRLTPDCRLKGKLAAARQ
                     AFYGELDRSTLADCAYPARREGQNIKSPVLASS"
     gene            1170..1640
                     /locus_tag="PKV23_08055"
     CDS             1170..1640
                     /locus_tag="PKV23_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012109886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85426.1"
                     /translation="MDWITITRALHLIAIVHWIGGLAMVTLVILPGIMRMPQERKLET
                     FLAIEERFAGQAKVSVTVAGLTGLYMVHGLVAWERFLDPGYWWMHAMVLVWAIFTFML
                     FAAEPLFLHAKVARMAERDPAATFSLMLRAHRILLALATVTVGGAALGAHGMLF"
     gene            complement(1662..>2122)
                     /locus_tag="PKV23_08060"
     CDS             complement(1662..>2122)
                     /locus_tag="PKV23_08060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HQX85427.1"
                     /translation="PWWILTNYPDQSDMMSPLDGGLMIAYILATALVTGLAVSAALAV
                     AARSLGRFETRKFHHLAQCLIPIAGGGVFLGLSALTVTMLRAEGLALDFVGVMRACLL
                     AGAAAWSLWLSWLVCRHYGASLPRRIAAVAAVACAVMVGTANWIALFWIW"
BASE COUNT          456 a          609 c          604 g          453 t
ORIGIN      
        1 gcttcagatc cttgagattc tcctcagcca tgagcgcata tgcagccaga cttggctgtc
       61 agttcgaaac gcgggaacag gacatcgttt tcgacccgaa tgtgagcgcg aatatcctca
      121 caaagctttt tcaggccggc ataaagcctc tgccatgagc cgcaagcgcc ttcaggcact
      181 gcgaatcccc tggccagttc ctccagtcgg cagatagtgc ctgcgtgatc ctcatgttcg
      241 tggcgcatca tggtgatcgg ctgcgcaagg ccttcgttga cgccctgccg catcgccgga
      301 aacaggacaa attcttcctt gcgcatgtgt gtgatcaatt cgtcggacag acgctcaaga
      361 acatcagcca gaccgagcgg cgcctcgggc acatcatgat gaacgcgctc gactttgcgg
      421 gcaagagcca ggagctcagg caattcttgc cggtgaatct cgtgatagtg cgacacgata
      481 tggtcaatga gtgctgccac gttcagagcc gcaagctgtg gaacccaatt ggcctgcttc
      541 gtttcaatgg tcatgacatc tatccttccc ggcagttccg caaggctttg ttgacctcac
      601 gggatggcct gccaatggtc agttcttaat acatattccg aataccactt tacaaattta
      661 taatgtatat tgcaaatgca attttgcctg gataccggag aaaactcgtg cgcctgactg
      721 aatttaccga ctttggcctc cgcgccctga tgcggctggc agccgagccc gagcgcgttt
      781 ttacgaccga tgaaattgcc aatgaatttg caatttcgcg gaaccatctg atgaaaatca
      841 tacataatct tgccaaagcc ggaattgtcg taaccctgcg cggggtcggc ggcggcttca
      901 ggctgagcaa gtccccggaa gagatacctc tcggcgggct tgtccggctt cttgagcagc
      961 gccatgcact ggtcgagtgc ttccaggcgg atggaggcca atgccggctg acaccagact
     1021 gccggctgaa gggaaagctg gcagcggcac gccaggcttt ttatggcgaa ctcgaccgct
     1081 caacgctcgc cgattgcgct tatcctgcga ggagggaagg ccaaaatatc aagtcaccgg
     1141 tcctggccag cagttgaggt aggagcgata tggattggat caccatcaca cgagcccttc
     1201 accttatcgc catcgtacac tggatcggcg gtttggcgat ggttacgctc gtcatccttc
     1261 cgggtatcat gcggatgccg caggagcgga aacttgaaac ttttcttgcc atagaggaac
     1321 gcttcgcggg acaagcgaag gtttcggtga cggtggctgg attgaccggt ctctatatgg
     1381 tccatggcct ggttgcttgg gagcgctttc tggatcccgg ttactggtgg atgcatgcta
     1441 tggtgctggt ctgggcgatc ttcaccttca tgctgttcgc cgcagagcca ctcttcctcc
     1501 atgcgaaggt tgcacgaatg gcggaacgcg atcctgccgc cacattttcc ctgatgctgc
     1561 gggcgcaccg gatcctgttg gcccttgcga ccgtgacggt tggaggtgcg gctctcgggg
     1621 cgcacggcat gttgttttaa gttggagcga aaggagggat ttcaccaaat ccagaatagc
     1681 gcgatccaat tcgccgtgcc aaccatgacg gcacaggcca ctgccgcaac cgcggcaatc
     1741 cggcgcggca gtgacgcccc ataatgacgg cataccagcc acgaaagcca tagggaccac
     1801 gccgcggcgc ccgcaagcag gcaggcgcgc atgacgccca cgaaatcaag cgccaggcct
     1861 tcggcgcgca gcatcgtcac cgtcaaggcc gacaggccga ggaagacccc acctccggca
     1921 atcgggatga ggcattgggc caggtggtga aattttctcg tttcaaagcg gccaagggaa
     1981 cgggccgcca cagcaagtgc cgcactcaca gccaatcctg taaccagtgc cgtggccagg
     2041 atgtatgcaa tcatgagccc gccatccaat gggctcatca tgtcgctctg atcgggatag
     2101 tttgtgagta tccaccaagg cc
//
LOCUS       DAORVN010000390         2118 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_55167_length_2118_cov_3.144450, whole genome shotgun sequence.
ACCESSION   DAORVN010000390 DAORVN010000000
VERSION     DAORVN010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55167_length_2118_cov_3.144450"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..441)
                     /locus_tag="PKV23_08065"
     CDS             complement(<1..441)
                     /locus_tag="PKV23_08065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85428.1"
                     /translation="MSRDELRQPLRKRSLNERLWARRPTPLLATSFLAAAGFIALSAW
                     AISTPHPFAGEPIITAAIPAVEEFKTASTTAPEEEAPAEDTPVDEGGVSVQIEAPVEQ
                     DSYQTEASIILSPRRPLKPAPIADLIEISTMGQLPRIGPGNRKPS"
     gene            complement(653..2005)
                     /locus_tag="PKV23_08070"
     CDS             complement(653..2005)
                     /locus_tag="PKV23_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016920106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HQX85429.1"
                     /translation="MKLLNNGVRSFALVLAGAVSATVLWHVFGSAIAASNADTYKQLN
                     LFGDVFDRVRADYVEDPDESKMIEAAINGMLTSLDPHSSYMDAKRFEDMSVQTRGEFG
                     GLGIEVTMENGFVKVVAPIDDTPAAKAGIMANDLITKIDGVDIQGLTLNEAVDKMRGG
                     VNTPITLTIQRKDQASMDVKLIRDRIRVQSVKSNLEGAEEDIGYVRISSFTEQTQEGL
                     EKAFTSLKTRMGGKAKGYILDLRNNPGGLLDQAISVSDSFLDKGEVVSTRGRHAEETQ
                     RFSAQKGDISDGKTVVILINGGSASASEIVAGALQDHKRATIIGTRSFGKGSVQTIIP
                     LGTQGALRLTTARYYTPAGRSIQARGIDADILIEQPLPPELQGKNVSTEGEASLKGHL
                     TNEAGGDEGGGSSAYVPEDKTKDVQLKAAVDFLHGIKVVSNVKPEETKADGSPETKSN
                     "
BASE COUNT          389 a          599 c          661 g          469 t
ORIGIN      
        1 ggagggcttc cggttgccgg ggccaatgcg gggcaattgc cccatggtgg atatttcaat
       61 gagatcggca atgggtgccg gcttcagggg ccgccggggc gacaggatga tggaggcttc
      121 ggtctggtag ctgtcctgtt cgaccggagc ctcgatctga acgctcacgc cgccttcgtc
      181 aacaggagta tcttcggcag gcgcctcttc ttcaggcgcg gtggtggagg cggtcttgaa
      241 ctcctcgacg gccggaatgg cggcggtgat gatgggctcg ccggcaaagg gatggggagt
      301 ggaaatggcc caggcagaca gtgcgataaa cccggccgcg gcgaggaagg aggtagcgag
      361 aagcggtgtg ggacgcctgg cccaaaggcg ctcattcagg ctccgtttcc tcagcggttg
      421 tcgcagttca tcacggctca tgaaattctg gtgcccagtc gattcgtttg gactcaactg
      481 tggcgaaaca accgttaacg caatgttaac cctgctacaa agttttccat cattttctaa
      541 tcctgcccgt gttggtcgtg gccatactca ccgcttgaaa aaagatggcc gggttgatcc
      601 ccggatcaag tccggggacg gccatgatga gagtctggat tggttgataa gatcagttcg
      661 acttggtttc cggcgagccg tcagcctttg tctcttccgg cttcacgttg ctcaccacct
      721 tgatgccgtg gaggaaatca acggcggcct tcaactgcac gtccttggtc ttgtcctcgg
      781 gcacataggc cgatgagccg ccgccttcat cgccgccggc ctcattggtg aggtggccct
      841 tgaggctggc ttcgccctcg gtggagacgt tcttgccctg gagctccggt ggcaggggct
      901 gttcgatcag gatatcggca tcaatgcccc tggcctgaat ggaacggccg gcgggcgtat
      961 agtagcgcgc cgtcgtaagt cgcagcgccc cctgggtgcc cagcggaata atggtctgga
     1021 cggagccctt gccgaaggag cgggtgccga tgatggtggc gcgcttgtga tcctgcaggg
     1081 cgcccgcaac gatttcagag gccgaggccg agccgccgtt gatcagaatg accaccgtct
     1141 tgccgtccga tatatcgcct ttctgggcgc tgaagcgctg ggtttcctcg gcatggcggc
     1201 cgcgggtcga gacaacctca cccttgtcga ggaaactgtc ggaaacggaa atggcctggt
     1261 cgagaaggcc gcccggattg ttccggagat caaggatgta gcccttggcc ttgccgccca
     1321 tcctggtttt caggctggtg aaagcctttt cgaggccttc ctgggtctgt tcggtgaagg
     1381 agctgatgcg gacgtagcca atgtcctctt cggcgccttc gagattggat ttcaccgact
     1441 gaacgcgaat gcggtcgcgg atgagcttca catccatcga cgcctggtcc ttgcgctgga
     1501 tggtgagggt gatgggcgtg ttcacgccgc cgcgcatctt gtcgacagcc tcattcaggg
     1561 tgaggccctg gatgtccacg ccgtcgatct ttgtgatcag gtcgttggcc atgatgcccg
     1621 ccttggcggc cggcgtgtcg tcaatggggg cgacaacttt cacaaagccg ttttccatgg
     1681 tcacttcaat gccgaggccg ccgaattcgc ccctcgtctg gacactcatg tcctcaaagc
     1741 gcttggcgtc catgtagctg gaatggggat cgagcgaggt cagcatgcca ttaatggccg
     1801 cctcaatcat cttggattcg tcgggatctt ccacatagtc ggcgcgcacc cggtcaaaca
     1861 cgtcgccgaa aagattgagc tgcttgtagg tatcggcgtt ggaggcggcg attgccgagc
     1921 caaagacatg ccacagcacg gtagccgaaa cggcccctgc aagcaccaga gcgaaagacc
     1981 gtacgccgtt attgagtagt ttcattttta aaagcctctt tcgttccacc aatccaccag
     2041 ggggccgaat caacggcctc gccattcttt ctaaattcga catatagcac aggccgtgcc
     2101 tcttgtacct gatcaccc
//
LOCUS       DAORVN010000391         2117 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_55228_length_2117_cov_2.435500, whole genome shotgun sequence.
ACCESSION   DAORVN010000391 DAORVN010000000
VERSION     DAORVN010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2117
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55228_length_2117_cov_2.435500"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..887)
                     /locus_tag="PKV23_08075"
     CDS             complement(<1..887)
                     /locus_tag="PKV23_08075"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HQX85430.1"
                     /translation="MIGNLLFLALFVGLTFLFGWLTWRAIRASKLWVKIAGSIGAGLL
                     TALCAALAFLGGKGLAAMYLPGANPAPDITVAGTADQIARGEYLVNLSCIGCHSAVGA
                     DGRPTEAHPLSGGWNLSASEGFGFIGDMVTENLTPGGKLAGYSDGEIFRALRHGVNQD
                     GQLLAMMSLLTYNQLSNEDTEAIVAYLRTLDATATDGATGDNINYLGDVMFGAGMFGQ
                     PTGPAAPDTITAPPQGVTAEYGKYVATFGECRGCHGPDVTGTEASATGPAIPNPRLLV
                     ATVSQERFFTMMRSGVKPDG"
     gene            complement(923..1264)
                     /locus_tag="PKV23_08080"
     CDS             complement(923..1264)
                     /locus_tag="PKV23_08080"
                     /inference="COORDINATES: protein motif:HMM:NF012355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HQX85431.1"
                     /translation="MKRRSWFSLLSIFVLLLAACGSSNDVTIKTEGMHFVKDEVRVKA
                     GQPVTLRVVNQDGYAHAFDLDEFDIHAPLSAQATFGAVFTPTAPGRYRFYCGSPGHAT
                     AGMVGVLVVEP"
     gene            complement(1334..1729)
                     /locus_tag="PKV23_08085"
     CDS             complement(1334..1729)
                     /locus_tag="PKV23_08085"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009464583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HQX85432.1"
                     /translation="MERRIINPWTWQDEFGYVQANEVSGIQRILYCSGQGANDVSGAA
                     VLAGDLRGQIGLALDNLETLLSAAGFSFADIVRLNIYTTDVDQFFADYDAFIGRLAEA
                     GCRHTGSLIGVTRLAFPEMMVEIEATAVA"
     gene            complement(1742..1972)
                     /locus_tag="PKV23_08090"
     CDS             complement(1742..1972)
                     /locus_tag="PKV23_08090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85433.1"
                     /translation="MNTSPGKRTMHYHSFILTLWQEGETMPNAPTIWRYSLANPHTGE
                     RIGFRDAVELGRFLTQWVALLPPNTNADEITN"
BASE COUNT          450 a          638 c          596 g          433 t
ORIGIN      
        1 ccgtcaggtt tgacgccgct gcgcatcatg gtaaagaatc gctcctggct caccgtggcg
       61 acgagcaggc gcgggttagg gatggcaggc cccgtagccg acgcttctgt ccccgtgaca
      121 tccgggccgt ggcagccgcg gcactcgcca aaggtcgcca cgtatttgcc gtactcggcg
      181 gtcacaccct gaggcggcgc cgtaatcgtg tcgggcgcag ccgggcctgt cggctgtcca
      241 aacatgcctg caccgaacat cacgtcgccc agatagttga tgttgtcgcc cgtagccccg
      301 tcggtggctg tcgcatccag cgtgcgcaga taggcaacga tcgcctcggt gtcttcgttg
      361 ctcaattggt tgtaggtgag aagcgacatc atggcaagca attggccatc ctgattgacg
      421 ccgtggcgta gagcccgaaa gatctcgccg tcgctgtatc cagctaactt gccgcctggg
      481 gtcaggttct ccgtcaccat gtcgccgatg aaaccaaagc cttcagaggc ggagagattc
      541 cagccgccgc tcaacggatg cgcttcggtt ggcctcccgt ccgcgcctac ggcgctgtgg
      601 cagccaatgc aggagagatt caccaagtat tcgccgcggg cgatctgatc ggccgtgccc
      661 gccaccgtga tatccggcgc agggttagcg cccggaaggt acatggccgc taacccttta
      721 ccccccaaaa aggcgagggc ggcgcagagc gcagtcagca acccggcgcc aatgctgccg
      781 gcgattttta cccacagttt gctggcccga atggcgcgcc aggtcagcca gccgaacaag
      841 aatgtcagtc ctacaaataa ggccaagaac aagagattgc cgatcatgtg agttcctcct
      901 caagtatcca gaatacgaac gattacggtt cgaccaccag cacgccgacc atgccggcgg
      961 tcgcgtgacc gggcgagccg cagtagaaac gatagcgccc cggcgcagtg ggcgtgaaga
     1021 ctgcgccaaa ggtcgcctgg gccgacagcg gtgcgtgaat gtcgaattcg tcgaggtcaa
     1081 aggcatgggc gtagccatcc tggttgacta cccgcagcgt caccggctga ccggccttga
     1141 cccgcacttc atccttgaca aagtgcatcc cctcggtttt gatggtgaca tcgttgctcg
     1201 aaccgcaggc cgccagcaac aggacaaaaa tggacagcaa ggaaaaccag gatcgccgct
     1261 tcatgattga gcctctttcg tagtcatggg agatgcacag tttgttctcc ttgaacttta
     1321 taaagatatt gaatcaagcc actgcggtcg cttcgatctc caccatcatc tccggaaagg
     1381 ccaaccgagt cacgccgatc agcgaaccgg tgtggcggca gccagcctca gcaagtcgcc
     1441 caatgaacgc atcgtaatcg gcgaaaaact gatctacatc ggtggtgtag atgttcagac
     1501 gcacaatatc ggcaaagctg aagcccgctg cactcagcag ggtctccagg ttgtccagcg
     1561 ctaagccgat ctgtccgcgc aggtcgcctg ccaacaccgc cgcccccgat acatcattgg
     1621 cgccctgccc agaacaatag agtatgcgct gtataccgct cacttcgttg gcttgcacat
     1681 agccaaactc atcctgccac gtccacgggt tgatgatacg tcgctccatg ttcattcctc
     1741 cctaatttgt gatttcatca gcattcgtat tgggcggcag cagcgccacc cactgggtaa
     1801 gaaagcgacc aagctcgaca gcatcgcgaa agccgatgcg ctcgccggtg tgcggattgg
     1861 ccaagctgta ccgccaaatg gttggtgcgt taggcatcgt ctctccctcc tgccaaagtg
     1921 taagaatgaa gctgtggtaa tgcatggtgc gtttccctgg gctggtattc atcgtttcac
     1981 ccttgcttgc ggcctgtggt acgctatgcc cattctacac agtaatcatt tagaaatcat
     2041 ttgttggaga catggatgac ggccgatttg caaattcggt tgtttggtgg cctggaaatt
     2101 cgccaaggca acgttcc
//
LOCUS       DAORVN010000392         2117 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_55240_length_2117_cov_1.978661, whole genome shotgun sequence.
ACCESSION   DAORVN010000392 DAORVN010000000
VERSION     DAORVN010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2117
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55240_length_2117_cov_1.978661"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..277)
                     /gene="rpsU"
                     /locus_tag="PKV23_08095"
     CDS             complement(<1..277)
                     /gene="rpsU"
                     /locus_tag="PKV23_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009799172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HQX85434.1"
                     /translation="MQVVVRDNNVDQALKVLKKKMQREGIFREMKLRGHFEKPSEKKA
                     REKAEAVRRTRKLARKKLQREGLLPMPKKIEKPKRFGAGGGAGAGGAF"
     gene            complement(370..1452)
                     /locus_tag="PKV23_08100"
     CDS             complement(370..1452)
                     /locus_tag="PKV23_08100"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018181482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HQX85435.1"
                     /translation="MRLEKPLAPGSTIGILGGGQLGRMLALAAGELGFSCHIFAPEAD
                     SPAFQVSGAHTVAGYHDFTALEGFAESVAVVTYEFENVPGDTAAFLESRVALAPSSGA
                     LKTAQDRVDEKTFIAGLGIPVAPFAAVNGQPELEAAIALIGRPSVLKTRRFGYDGKGQ
                     TKITEKTSAAAAWAEIGKAPAILEGFVSFDKEISVIAARGWDGAIAVYDVPENRHENH
                     ILKTSTVPADIAGETSEAARAMARRLIGALDYVGVMGIEMFVSGSNLIVNEIAPRVHN
                     SGHWTQDACVVSQFEQHIRAICGWPLGSTERHSNVVMHNILGGESANWADYGASAHTG
                     VHFYGKAEARRGRKMGHINVLTPRQG"
     gene            complement(1452..1916)
                     /gene="purE"
                     /locus_tag="PKV23_08105"
     CDS             complement(1452..1916)
                     /gene="purE"
                     /locus_tag="PKV23_08105"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018429924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HQX85436.1"
                     /translation="MGSQSDWATMKHAAEILDVLGVAHDSRIVSAHRTPKRLYDFAGG
                     AKAEGIKVIIAGAGGAAHLPGMVAALTTLPVLGVPVESKALSGQDSLLSIVQMPAGIP
                     VGTLAIGKAGAINAGLLAASVLALSDKALAKRLEAWREKQTAGVAITPKDDA"
     gene            complement(1954..2115)
                     /locus_tag="PKV23_08110"
     CDS             complement(1954..2115)
                     /locus_tag="PKV23_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006932168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HQX85437.1"
                     /translation="MLQQHRDLDVAIASLAETGTSDQLQLRRLKKMKLDLKDQIQKIE
                     NMLIPNIIA"
BASE COUNT          406 a          709 c          567 g          435 t
ORIGIN      
        1 agaacgcgcc gccagctccg gcaccgcccc ctgcgccaaa gcgcttgggc ttctcgatct
       61 tcttgggcat gggcagcagg ccttcgcgct gcagcttctt gcgggccagc ttgcgggtgc
      121 ggcgcaccgc ctccgccttc tcgcgggcct tcttctccga cggcttttca aaatgcccgc
      181 gcagtttcat ctcgcggaaa atgccctcac gctgcatctt tttcttgaga accttcaggg
      241 cctggtcgac attgttgtca cggactacga cttgcaagtg ctacctcgaa tttggtcaaa
      301 gttgaactgg tttaaaaacg gaatccctat tgggagttcc caaaagtggg cgtggcgtac
      361 cacagcccgt catccctgtc gaggggtcag cacgtttata tggcccattt tgcgcccccg
      421 ccgggcttcc gccttgccat agaaatgcac cccggtatgg gccgaagccc cataatctgc
      481 ccaattggcg ctttccccgc ccaatatgtt atgcatcacc acgttggaat gccgctccgt
      541 cgagcccagc ggccagccgc aaatggccct gatatgctgc tcgaattgcg aaaccacgca
      601 ggcatcctgg gtccaatggc cggaattatg cacccgcggc gcgatctcgt taacgataag
      661 gttgctgccc gatacgaaca tctcaatccc catcacccct acatagtcca aagctccgat
      721 gagcctccgc gccatggcgc gcgccgcttc cgacgtttcc cctgcgatat cagcgggaac
      781 cgttgaagtc ttgagaatat ggttttcgtg ccggttttcc gggacatcat aaaccgcaat
      841 cgccccgtcc cagccccgcg ccgcaatcac cgagatctcc ttgtcgaagg aaacgaagcc
      901 ctcgagaatg gcgggcgcct tgccgatttc agcccaggcg gcggccgccg aagtcttttc
      961 cgtgatcttc gtctggcctt tgccgtcata accgaaccgc cgcgttttca aaaccgaagg
     1021 cctgccgatg agggcaatgg ccgcttccag ttccggttgg ccattcaccg cggcgaaagg
     1081 cgcaaccgga atgcccaggc cggcgatgaa ggttttttca tcaacccggt cctgcgccgt
     1141 cttgagcgct cccgaactgg gcgccagggc cacgcgggat tcaagaaacg ccgccgtgtc
     1201 gcccggaaca ttttcaaact cgtaagtgac gacagcaacg gactctgcaa agccttccag
     1261 cgccgtaaaa tcatgatacc ccgcaaccgt gtgggcgcct gaaacctgaa atgccgggct
     1321 gtctgcctcc ggcgcaaaga tatggcagga aaagccaagc tcgcccgccg ccagcgccag
     1381 catccgcccc aattgcccgc cgcccagaat gccaatcgtt gaaccaggcg ccagaggttt
     1441 ttccaggcgc atcaggcatc gtccttgggc gtaatggcaa cacccgccgt ctgtttctcg
     1501 cgccaggcct caagcctttt ggccagcgcc ttgtcgctca atgccagtac actggccgca
     1561 agcaggcccg cattgatggc gcccgccttg ccgatggcca gggtgcccac cggaattccg
     1621 gccggcattt gcacgatgga aagcagcgag tcctgccccg acagcgcctt ggattcaacc
     1681 ggcacgccaa gaaccggaag cgtcgtgagt gccgccacca tccccggcag gtgcgctgcc
     1741 ccgccggccc cggctatgat caccttgatt ccttccgcct tggcgccccc ggcgaaatca
     1801 taaagccgct tgggcgtccg gtgcgccgag acgatccgcg agtcatgtgc aacgccaagc
     1861 acgtcaagaa tctccgcggc atgtttcatc gtggcccagt ccgactggct tcccatgaca
     1921 atggcaacag gtgctttcaa aatctccggc ctttcacgca atgatgtttg gaatcagcat
     1981 gttctcgatc ttctgtatct ggtctttcag gtcgagcttc atttttttga gccgccgcaa
     2041 ctgcagctgg tcgctcgttc cggtctccgc cagcgaggca atggccacgt caaggtcacg
     2101 gtgctgctgc agcaaat
//
LOCUS       DAORVN010000393         2115 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_55320_length_2115_cov_2.583981, whole genome shotgun sequence.
ACCESSION   DAORVN010000393 DAORVN010000000
VERSION     DAORVN010000393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2115
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55320_length_2115_cov_2.583981"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(189..1094)
                     /locus_tag="PKV23_08115"
     CDS             complement(189..1094)
                     /locus_tag="PKV23_08115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85438.1"
                     /translation="MRKSLITSLVLHASILVAALVVLPDPDKYDVKPQDAIQVDISQI
                     SDESKRKATSKTAEKPIEKVAPKKAEVVKKVEPAPKVDDKVKTAAKEPVAEPPPPAPK
                     VVEPKKEEPKPLDPDPLKDLLKKVEEEPKPKEEVKKAEVKPPKKPEEKKPKKKKPELN
                     TDDIAAFLNKTDDEKTAPQQPMDLAALPELGDAEVQGTDDAMAATLIDALRQKLAGCW
                     SVPPGAREANITVKIRFNLTPDGSVSGYPEVLNGSGDPLFATTAQSAISAVMECQRYD
                     FMPPDRYDLWKDITVNFNPNMMFGT"
     gene            complement(1097..1567)
                     /gene="tolR"
                     /locus_tag="PKV23_08120"
     CDS             complement(1097..1567)
                     /gene="tolR"
                     /locus_tag="PKV23_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013167819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolR"
                     /protein_id="HQX85439.1"
                     /translation="MAAASGGAKGGYTRRNSRRRQSAGVMHEINVTPLVDVMLVLLIV
                     FMVAAPLMTVGVPIELPKTEAKQMDSSVEPLTITVQADRTIFLQETQIPLEELQPKLQ
                     AIAKNGYEEQIFVRADTNVSYGSVMEVMGLLNAAGYRKIGLVTGNIEKKAEIAN"
     gene            complement(1570..>2115)
                     /locus_tag="PKV23_08125"
     CDS             complement(1570..>2115)
                     /locus_tag="PKV23_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011475153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HQX85440.1"
                     /translation="RMNAANDRFEQSFWSGQSLEDLYLALGTRPNLGLSAIFMAAMRE
                     WKRSQDAALRAGFQGVQKRIEKVVDVQIQKEMSTIEARLLFLATTGAAAPFIGLFGTV
                     WGIMTSFQAIANSQNTNLAVVAPGIAEALFATAIGLVAAIPATIFYNMFSADAGRIGM
                     RLENFADEFSAIISRQLDERV"
BASE COUNT          380 a          638 c          595 g          502 t
ORIGIN      
        1 cgccccgccg cccaccagga aaggcgtgat ggcgattggc aacgggttga tgcgcccgcc
       61 ggatacgtcc acttccagcg cgtggctggt ccgcggcaca agggccagaa cgaggaatga
      121 aagcatcaca agaaaatttc tcatgggcgc aaggctggca gagttgggca ttgaaatttc
      181 catggggctt atgttccaaa catcatgttt ggattgaaat tgacggtaat gtctttccac
      241 aggtcgtagc ggtcgggcgg catgaagtca tagcgctggc attccataac cgctgaaata
      301 gccgattgcg ctgtcgttgc aaaaagcgga tcgccgctgc cgttcagcac ttccggatag
      361 cctgacaccg agccgtcggg cgtgaggttg aagcggattt tgaccgtgat gtttgcttcc
      421 cgcgcgcccg gcggtaccga ccagcagccg gccagcttct ggcgaagtgc atcgatgagg
      481 gtcgccgcca tggcgtcatc cgtgccctga acctctgcgt cgcccagttc cggcaatgcg
      541 gcaagatcca tgggttgctg cggcgccgtc ttctcgtcgt ccgtcttgtt caggaaggca
      601 gcgatgtcat cggtgttgag ttccggcttt ttcttcttcg gcttcttctc ctccggtttc
      661 ttcggaggct tcacttccgc cttcttgacc tcctctttcg gcttcggctc ctcttccacc
      721 ttcttgagaa gatccttcag cggatccgga tcaagcggct tgggctcttc ctttttcggc
      781 tcaacgacct tgggtgcggg cggcggcggt tcagccacgg gttccttggc ggcggtcttc
      841 accttgtcgt caacctttgg cgccggctcg accttcttca ccacttccgc cttcttgggc
      901 gcaacctttt caatgggctt ttcagcggtc ttcgaggtcg ccttgcgctt tgactcatcg
      961 ctgatctggg aaatatcaac ctggatggcg tcctgcggct tgacgtcata tttgtcggga
     1021 tcaggcagga ccacaagtgc cgcgaccaga atcgaggcat gcaataccag ggatgtgatc
     1081 aggctcttgc gcatgtctag ttggcgatct ccgctttctt ctcgatattg cccgtcacca
     1141 ggccgatctt gcggtagcct gccgcgttga gcaggcccat gacttccatg accgaaccat
     1201 agcttacatt tgtgtcggcc cgcacgaata tctgttcctc atagccgttc ttggcaatgg
     1261 cctggagttt cggctgaagc tcctcaagcg gtatctgggt ctcctgcagg aaaatggttc
     1321 tgtccgcctg caccgtgatg gtaagcggct cgacgctgct gtccatctgc ttggcttcgg
     1381 tcttgggcag ctcgatgggc acgccgactg tcatcagggg tgctgccacc atgaacacga
     1441 tcagcagcac cagcatcacg tcgacaagtg gcgtcacgtt gatttcatgc atcacgccgg
     1501 ccgattgccg ccgccttgaa ttgcgccgcg tatatccgcc cttggctccg cctgatgctg
     1561 ctgccatgcc taaacccgtt catccagctg acgcgaaata atcgccgaga attcatcagc
     1621 aaagttttcc agacgcatgc cgatccggcc tgcatcggcc gagaacatgt tgtaaaagat
     1681 tgtcgctggt atggcggcca caaggccgat ggccgtggca aacagggctt ccgcgatgcc
     1741 cggcgccacc acggcaaggt tggtgttctg cgaattggcg atggcctgaa acgaggtcat
     1801 gatgccccat actgttccga acaacccgat aaagggtgcc gccgcccccg ttgttgccag
     1861 gaacaggagc cgcgcttcaa tcgtgctcat ttccttctgg atctgcacat cgacgacttt
     1921 ttcaatgcgt ttctgcacgc cctggaagcc ggcccgaagc gccgcatcct ggctccgctt
     1981 ccattcgcgc attgccgcca tgaaaatcgc cgaaaggccc agattgggac gcgttccaag
     2041 ggcgagatag agatcctcca gcgattggcc cgaccagaag ctttgctcga accggtcatt
     2101 ggccgcgttc atccg
//
LOCUS       DAORVN010000394         2114 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_55354_length_2114_cov_3.218553, whole genome shotgun sequence.
ACCESSION   DAORVN010000394 DAORVN010000000
VERSION     DAORVN010000394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2114
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55354_length_2114_cov_3.218553"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1786)
                     /locus_tag="PKV23_08130"
     CDS             complement(<1..1786)
                     /locus_tag="PKV23_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008188575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HQX85441.1"
                     /translation="MPLHPPSDKPWLFRTYAGHSTAAESNRLFRSNLEKGQTGLSIAF
                     DLPTQTGYDPDHLLARGEVGKVGVPVSHIGDMRALLDGIPLATMNTSMTINATAAWLL
                     ALYVAVADGQGIPRNQLSGTTQNDIIKEYLSRGTHVFPPRPSLKLTTDTIAFSASDLP
                     KWNPMNVCSYHLQEAGATPVQELAFALATALAVLDAAKTEVSAAAFPEVVGRMSFFVN
                     AGIRFITEMCKMRAFTELWDEICRERYAIAEEKHRRFRYGVQVNSLGLTEQQPENNVY
                     RILLEMLAVVLSKNARARAVQLPAWNEALGLPRAWDQQWSLRMQQIVAYETDLLDYAD
                     IFEGSKEIEAKVESLKAEARTELQKILALGGAIPAIESGYMKRALVQANTERLKAIER
                     GDTKLVGVNIFTTTETSPLSTGDGEKILTVSENVEFEQIEKLKAWRAARDKKAVATAL
                     AALREAAAAGHNIMEPSIACAKAGVTTGEWGETLRQVFGEFRAPTGVLLTMDQHPQKS
                     LEDVRARVRKLALELGMTPKFLMAKPGLDGHSNGAEQIAVRAADCGLDVTYDGIRLTP
                     EQIVMTAREKKVTLIGLSVLSGSHVPLVR"
     gene            complement(1862..>2114)
                     /locus_tag="PKV23_08135"
     CDS             complement(1862..>2114)
                     /locus_tag="PKV23_08135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85442.1"
                     /translation="MLTSLLFSAAGALLLSRFTGAIGQLTLPLNFSALFIGTALANIL
                     LDGFDVPAFQYEQEVLLFTLVGLIAGSFAMLWCVRPEHS"
BASE COUNT          433 a          572 c          674 g          435 t
ORIGIN      
        1 cgcgcaccag cggaacatgg ctgccggaga gcactgagag gccaatcagg gtgaccttct
       61 tctcccgggc ggtcatcaca atctgctcag gggtgaggcg gatgccgtca taggtgacgt
      121 caagcccgca atcggcggcg cgcaccgcga tctgctcggc cccgttggaa tggccatcga
      181 gaccgggctt ggccatgaga aacttgggag tcatgccaag ctccagggcg agtttcctga
      241 cgcgggcccg gacgtcctcc agcgatttct gcggatgttg atccatcgtc agaagcacgc
      301 cagttggggc gcggaattcg ccaaagacct ggcgcagggt ttcaccccat tcgccggttg
      361 tgacgcctgc tttggcgcaa gctatggaag gttccatgat gttgtggccc gccgcggcgg
      421 cttcgcgaag cgctgcgagc gctgtggcca cggccttttt atctcgcgcg gcgcgccatg
      481 ccttcagttt ttcgatttgc tcgaattcaa cgttttcgga tacggtgagg atcttctcgc
      541 catcgcctgt ggaaagcggc gaagtttcgg ttgtggtgaa gatgttgacg cccaccagtt
      601 tggtatcgcc gcgctcgatg gccttcaggc gttcggtgtt ggcctggacg agggcgcgtt
      661 tcatgtagcc cgattcaatt gccgggatgg caccacccaa tgcgagaatt ttctgcaatt
      721 cagttcgggc ttcggctttc aggctctcga ccttggcttc aatttccttc gagccttcga
      781 aaatatcggc gtagtcgagc agatcggttt cataggccac gatctgctgc atgcgaagcg
      841 accactgctg gtcccaggcg cggggcaggc caagcgcctc attccaggcg ggcaattgca
      901 cggcgcgggc cctggcattt tttgaaagta ccacggccag catttcaagg aggatgcggt
      961 agacattgtt ttccggctgc tgctcggtca ggccgaggga attcacctgc acgccatagc
     1021 ggaagcggcg gtgtttctct tcggcaatgg cgtaacgctc gcggcagatt tcatcccaga
     1081 gttcagtgaa ggcccgcatc ttgcacattt cggtaatgaa acggatgccg gcattcacaa
     1141 agaacgacat gcgcccaacc acttccggga aggccgcggc actgacttcg gtttttgccg
     1201 catcaagcac ggccaaagcg gtggccaatg caaaagccaa ttcctgaacg ggcgtggccc
     1261 ctgcctcctg caaatgatag gagcagacat tcatgggatt ccatttcggc agatcgcttg
     1321 cggaaaaggc aatcgtatcg gtggtgagtt tcagggaggg ccggggcgga aacacatggg
     1381 tgccgcgcga gagatattcc ttgatgatgt cgttctgggt ggtgccggac aactggttcc
     1441 gcggaatgcc ctgcccatct gctaccgcca cgtaaagggc gaggagccag gcggcggtgg
     1501 cattgatggt catcgaggtg ttcattgttg caagcggaat gccgtcgagc agggcccgca
     1561 tgtcgccgat atgggagacg gggacgccca ctttaccaac ttcaccgcgc gccaggagat
     1621 gatcgggatc gtagccggtc tgggtcggca ggtcgaaggc tatggaaaga ccggtctggc
     1681 ccttttcaag attggaacgg aacagccggt tgctttctgc agccgtggaa tggccggcat
     1741 aggtgcgaaa cagccagggc ttgtcggatg gcgggtgaag cggcattcag gcgtccattt
     1801 tgcgatgcaa tatggaagca cggttgtttt cgcggcgcaa ccctgccgca ggcggcataa
     1861 cctagctgtg ttcgggcctg acgcaccaga gcatggcaaa agagccggca atgaggccca
     1921 cgagagtgaa aagcaagact tcctgttcat actggaatgc cggaacatcg aagccgtcaa
     1981 ggaggatgtt ggccagagcc gtgccaatga agagggctga aaaattcagg ggcaaagtaa
     2041 gctggccgat agccccggta aacctggata aaagaagggc gcctgcagcg ctgaaaagca
     2101 gcgacgtcag catg
//
LOCUS       DAORVN010000395         2113 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_55415_length_2113_cov_2.282799, whole genome shotgun sequence.
ACCESSION   DAORVN010000395 DAORVN010000000
VERSION     DAORVN010000395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55415_length_2113_cov_2.282799"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..437
                     /locus_tag="PKV23_08140"
     CDS             <1..437
                     /locus_tag="PKV23_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014764922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HQX85443.1"
                     /translation="ILSGVNFCLHAAGWLEGGLASGYEKLIMDADQLTMFQKLAEGAD
                     FSENGQAMDAIREVGPGSHYLGAAHTRANFETAFWRSGLADNNSFEQWRDEGEKDIVV
                     RANTQWKKMLADYVAPPLDPGIDEALQTFMAKRKEVLPDSVS"
     gene            complement(440..1369)
                     /locus_tag="PKV23_08145"
     CDS             complement(440..1369)
                     /locus_tag="PKV23_08145"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HQX85444.1"
                     /translation="MERHRVLKGYLFAAGGAALFSTKAIFIKLAYMEEANAALMLALR
                     MATALPFFIGVGLYAVYQLRQAGKPLPGWGLTFRALLAGFIGYYISSLLDFEALVYIT
                     AQLERLVLFTYPMFVMFLGWLFFGARLTWMSIVAAVITYVGLVIVFLTDLPAGGIDTV
                     IGTALVLGCALTFAIYQLLAKNFITAMGSALFTSIALSGSGFACIIHYILVSRSFDFS
                     SSPRFFWLAAGTGFFATVLPSFLVNAGLSRISPQSTSMIATISPLITISLAVWILGEP
                     FTLVDAIGSAMVIAGVGFYALTDVKSKAPSETT"
     gene            1457..>2113
                     /locus_tag="PKV23_08150"
     CDS             1457..>2113
                     /locus_tag="PKV23_08150"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HQX85445.1"
                     /translation="MTELRFSSALEMARLLRARKISAKELLEVCLDQYALHNDTLNAV
                     VVTDLGRAKHAAMASDKRLKKGTPLGPFDGVPMTAKESFDWKGTPSTWGEPAYKENIA
                     TSDAVAITRLTNAGAVLYGKTNVPLMLADWQSFNAVYGTTNNPWDVTRAPGGSSGGSA
                     VALATGMSALEIGSDIGASLRNPAHYCGVYAHKPTYGVVPYRGHLLSNVQPSDISVAR
                     P"
BASE COUNT          465 a          604 c          673 g          371 t
ORIGIN      
        1 cgatcctctc cggcgtcaat ttctgcctcc acgcggcggg ctggctggaa ggcggattgg
       61 cttccggcta cgagaaactc atcatggatg cggaccagct caccatgttc cagaagcttg
      121 cagaaggtgc tgatttctcg gaaaacggcc aggccatgga tgccatccgc gaagtcggcc
      181 ccggttcgca ttacctgggt gccgcccata cgcgcgccaa tttcgagacc gccttctggc
      241 gctcgggcct tgccgacaac aattcattcg agcaatggcg cgacgagggc gaaaaggaca
      301 ttgtggtccg cgccaatacc cagtggaaaa aaatgctcgc cgactatgtg gcccctcccc
      361 tcgatcccgg catcgacgag gcgctgcaaa ccttcatggc caagcgcaag gaagttctgc
      421 cggacagcgt gagctgaggc taggtcgttt cgctgggcgc tttcgacttc acgtcggtga
      481 gggcgtagaa gcctacgccg gcgatcacca tggcggagcc gatggcgtcc accaaggtga
      541 agggttcgcc cagaatccag acggccagag aaatggtgat caacggggaa atcgtggcga
      601 tcatcgaggt ggattgtggc gagatgcgcg agagcccggc gttcaccagg aacgatggca
      661 ggacggttgc gaagaatccg gtgcccgccg ccagccagaa gaacctgggg cttgaggaga
      721 aatcaaagct ccggcttacc aggatataat gtatgatgca ggcgaaaccc gaacccgaga
      781 gggcaatcga ggtaaacaat gcactgccca tggcggtgat gaaattcttg gccagcagtt
      841 gatagatggc gaaggtcagg gcgcaaccca gcacgagggc ggtgccgatg actgtgtcga
      901 tgccgcccgc cgggaggtcg gtgagaaaca caatgacgag cccgacatag gtgatgaccg
      961 ccgccacaat actcatccag gtcagcctgg cgccgaaaaa cagccagccc aggaacatga
     1021 cgaacatcgg ataggtaaac aggaccaggc gctcaagctg ggcagtgatg tagaccagag
     1081 cctcgaaatc gagcagcgag gaaatgtaat agccgatgaa accggcgaga agggcgcgaa
     1141 atgtgaggcc ccagccgggc agcggctttc cggcctggcg gagctggtag acggcataaa
     1201 gcccgacgcc gataaagaag ggtaaggcgg tggccatgcg cagcgccagc atgagggcgg
     1261 cgttggcctc ctccatatag gcgagcttga tgaaaatggc cttggtggaa aacaaggccg
     1321 caccgcctgc ggcgaacagg tagcctttca agacgcgatg ccgttccatg gcccccgctt
     1381 taaaggctcg ccctgtactt gtctgcgtct ttgggtaaca tgactggcat gcacatacag
     1441 cgatggggcc tggttaatga ctgaattgcg tttttcttct gctcttgaaa tggcgcgcct
     1501 gctgcgggcc cggaaaatct cggcaaagga gcttctggag gtctgcctcg accagtatgc
     1561 cctgcataat gacacgctga atgcggttgt tgtcaccgat ctggggcggg ccaagcatgc
     1621 ggccatggct tccgacaagc gcctgaaaaa gggcacaccg ctggggccct ttgacggggt
     1681 gccgatgacg gccaaggaat ccttcgactg gaagggcacg ccctcgacct ggggcgagcc
     1741 cgcctataag gaaaatattg caacttcgga cgcggtggcc atcacccgcc ttaccaacgc
     1801 aggcgcggtg ctctatggca agaccaatgt gccgctgatg ctggcggact ggcagagttt
     1861 caacgcggtt tatggcacga ccaacaatcc ctgggatgtg acgcgggctc ccggtggctc
     1921 ctcgggcggc tcggcggtgg cgctggccac cgggatgagt gcgctcgaaa tcggttccga
     1981 tatcggcgcg tcgctgcgga accccgccca ttactgcggg gtttatgccc acaagccgac
     2041 ctatggcgta gtgccctacc gcggccatct gctgagcaat gtgcagccca gcgatatttc
     2101 cgtggcgcgc ccc
//
LOCUS       DAORVN010000396         2104 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_55841_length_2104_cov_4.063934, whole genome shotgun sequence.
ACCESSION   DAORVN010000396 DAORVN010000000
VERSION     DAORVN010000396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55841_length_2104_cov_4.063934"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="PKV23_08155"
     CDS             complement(<1..182)
                     /locus_tag="PKV23_08155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85446.1"
                     /translation="MRNLLVSCVLSVFCSIAQGGAYEDILNAASNDDTASVVSLLQRG
                     MDVNTADRAGNTLLMIA"
     gene            complement(175..957)
                     /locus_tag="PKV23_08160"
     CDS             complement(175..957)
                     /locus_tag="PKV23_08160"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011503124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HQX85447.1"
                     /translation="MNSLLVDSHCHLDFADFADREDELLAAMKSSGVGWALVAGVTLE
                     RFPTVLALVERFPNLYAAVGVHPDTQDGQDPDAATLIRLADHPRVVAIGETGLDYYRL
                     EGDLEWQRNRFRIHIRAARETRKPLIIHTREAAADTLRILEEEGASAVGGVFHCFTET
                     LDVAKAALALGFYISFSGIVTFKNALQIKEVARFVPLDRLLVETDAPYLAPVPHRGKL
                     NHPALVRHVAEEVAKLKGIGLEVLTDATTTNFFRLFGVDGNA"
     gene            complement(954..2009)
                     /gene="holB"
                     /locus_tag="PKV23_08165"
     CDS             complement(954..2009)
                     /gene="holB"
                     /locus_tag="PKV23_08165"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HQX85448.1"
                     /translation="MQRSDISNINWNTELRRQFLGQGLARLPHATLLIGPSGVGKAAF
                     AEQVAALLLCESPSSTMTACGECDSCRWLVADNHPDFRRVAPDDEGESESGQAEKSAE
                     KGKKRNPGIIRIDQIRDLEGFVFVGSHRNGNRVVLVTEAEAMNPSAANSLLKILEEPP
                     SSVYFLLISTRSKSLLPTLRSRCRVLAFGAPDTAAATNWLRGAGLEKQAKRYLGLAGG
                     APMRVAQWKEEGLLAPIDALVDSLLAPPADPGQLAARWDGLLKGDGQFRMEHLVEGVQ
                     RWLFDLAQERLTGEVRYHAGWPRPKGGNALNPMALLAAWRELGQFRRSARHPLNQLLF
                     LESMATHFLRVLRPATP"
BASE COUNT          475 a          600 c          616 g          413 t
ORIGIN      
        1 gcgatcatga gcagcgtatt gcccgcacgg tcggccgtgt tcacatccat cccccgctgc
       61 aaaagactga cgaccgatgc cgtgtcgtca ttgctggccg cattgagaat gtcttcgtag
      121 gcgccgcctt gggcaatcga gcaaaacaca gataaaacac atgagaccag caaattacgc
      181 atttccatcg actccgaata ggcggaaaaa attggtggtg gtggcatctg tcaagacttc
      241 caacccgatc cctttcagct tcgccacttc ttcggcgacg tggcgcacca gggccggatg
      301 attcagcttg ccgcggtgcg ggacgggcgc cagatagggg gcatcggttt cgacaagaag
      361 acgatcaagc ggaacgaagc gtgcaacctc tttgatttga agagcattct tgaacgtgac
      421 aatgcctgaa aaggagatgt aaaagccgag cgcaagggcg gccttggcaa cgtcgagagt
      481 ttcggtgaag caatggaata cgcctccgac ggctgaggcc ccctcctctt cgagtatccg
      541 caaggtatcg gcagcagcct cgcgagtatg aataatcaaa ggcttgcgcg tttcccgagc
      601 ggcgcggata tggatgcgaa accgattgcg ttgccattcc agatcaccct cgaggcgata
      661 gtaatcgagt ccggtttcgc cgatagctac gaccctggga tggtcagcaa gccgaatcag
      721 cgtcgccgca tccggatcct ggccgtcttg cgtatcggga tggacaccga ccgccgcgta
      781 tagattcggg aaccgctcga ccaacgcaag cacggtcggg aaacgttcca gcgtcactcc
      841 cgcgaccaga gcccaaccca cgccgctgga tttcatcgcg gccagcagtt cgtcctcccg
      901 atccgcaaaa tctgcgaaat cgagatggca atgggaatcg accagcaggg aattcatggt
      961 gtggcaggac gcaggacgcg caagaaatgg gtcgccatgc tttcgagaaa aagtaattga
     1021 ttcaaggggt gacgggcgct gcggcgaaac tggccaagct ctcgccaagc ggccaggagg
     1081 gccatcggat tcaaggcgtt gccccctttc gggcggggcc agccggcgtg ataacggact
     1141 tcgccggtca gacgttcctg cgcgagatcg aaaagccagc gctggactcc ttcgaccagg
     1201 tgttccatgc gaaactggcc gtcacccttg agcaaaccat cccatcgagc ggcaagttgc
     1261 cccggatcgg ccggaggagc aagaagggaa tcgaccaatg cgtcaatcgg tgccaatagc
     1321 ccctcctctt tccactgtgc tacgcgcatc ggggctccgc cggcaaggcc gagatagcgt
     1381 ttggcctgtt tttcgagccc ggcaccacgc agccagttgg tcgccgctgc tgtatcggga
     1441 gcaccgaaag caagcacacg gcaacggctg cgaagggtcg gtagcaggga ttttgatcga
     1501 gtagatatca ataaaaaata tacacttgaa ggaggttctt caagtatttt aagaagtgaa
     1561 ttggccgcag atggattcat cgcctcggct tccgtgacca aaacgacgcg gttgccgtta
     1621 cggtgactgc cgacaaaaac gaatccttcg aggtctcgaa tctggtcgat gcggataatg
     1681 ccgggatttc gcttcttgcc cttttctgcg ctcttctcgg cctggcccga ttcggactcg
     1741 ccctcgtcgt ccggtgcaac gcgccgaaag tctggatgat tgtcggcgac caaccagcgg
     1801 catgaatcgc attcaccgca cgcggtcatg gtggaagaag gcgactcgca aagcaggagg
     1861 gcggcgacct gctcggcgaa tgccgccttc cctacgccgg aaggacctat cagcaatgtc
     1921 gcatggggaa gccgggcgag gccctgcccg agaaactgcc tgcgcagctc ggtgttccag
     1981 tttatattcg atatatcaga tcgttgcaat tattatctca agttctttgt taatttcagc
     2041 cggagcccgg gttgcatcga cgacgcgcac ccgagcggga ttttcccgcg caatgcgcaa
     2101 gtag
//
LOCUS       DAORVN010000397         2101 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_55984_length_2101_cov_3.282014, whole genome shotgun sequence.
ACCESSION   DAORVN010000397 DAORVN010000000
VERSION     DAORVN010000397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55984_length_2101_cov_3.282014"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            268..891
                     /locus_tag="PKV23_08170"
     CDS             268..891
                     /locus_tag="PKV23_08170"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HQX85449.1"
                     /translation="MASYQLYCFGESGNAYKAALMLELCKADWQPIFVNYFNGQTRTP
                     EYRSEVNEMGEVPVLVDGAKKLTQSGVILDYLAGKTGEFGAKDDDERREILRWMFFDN
                     HKFTSYIATLRFMVSLAKTGETPVTEFLRQRFNTSLKIVDGHLAGQPFLIGKRPTIAD
                     FSLCGYMFFGAELPLPLEPYPHVLSWLERIKSLPGWKHPYDLMPREP"
     gene            complement(872..1507)
                     /locus_tag="PKV23_08175"
     CDS             complement(872..1507)
                     /locus_tag="PKV23_08175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85450.1"
                     /translation="MFIFGILFLAAGIALGLLIHFSPGKMQVRGLTPDVAAILIVGGA
                     LCLGLGSTIGALRDRTVAPVVVETAPETPEEKPATPKFTGFGKKAAATAAVAEASAAV
                     AETAAASKTSVADTVAALEQAKTDIAVALGVEPQKSAPQEFVVAKAEPEAKPEAADAE
                     ELYVVEEKVIRGRPARVLSDGTVEAETDEGWMRFENLEHLDEYLEAMVPEA"
     gene            complement(1612..>2101)
                     /locus_tag="PKV23_08180"
     CDS             complement(1612..>2101)
                     /locus_tag="PKV23_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006137959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HQX85451.1"
                     /translation="LREVSWGETDVMIVDMPPGTGDAQLTLAQQTPLSGAVIVSTPQD
                     LALIDARKGLNMFRKVNVPVIGIVENMSYFVCSKCGERHEIFGHGGAREEAARLGVPF
                     LGEVPLDKEVRIRSDSGEPVVVSMPDSLHATIYKDIARQVWAAVEGGKLSKPAPRISV
                     ES"
BASE COUNT          428 a          633 c          599 g          441 t
ORIGIN      
        1 gtcccagccg aggaaaggat ggagaaatcc ggaggcaatg ccatggcccg cgtgggcgaa
       61 agccggtgtt gccgaaactg aaaccagtgt tgcaataagt gctatgcgct tcattgttat
      121 gatccccttc atttggtctt gatcgaaaag caaactagca acggcggaga cgaaagtctc
      181 ccctcaaatg cgacatggct ggcttgttcc gcgccgttca tatccatcgt tgccatcctg
      241 atgcacatta aataggctgg ctccatcatg gccagctatc aactgtattg ctttggagaa
      301 tcgggcaacg cctacaaggc ggcgctgatg cttgaattgt gcaaagcgga ctggcagccg
      361 atcttcgtga actatttcaa tggccagacc cgcacgccgg agtaccgctc cgaggtaaat
      421 gaaatgggcg aggttccggt cctggttgac ggggcgaaaa agctaacaca atccggcgtc
      481 attctcgatt atctcgccgg aaaaaccgga gagttcggcg cgaaggatga tgacgagcgc
      541 cgcgaaatcc tgcgctggat gttcttcgac aatcacaaat tcaccagcta catcgccacc
      601 ctgcgcttca tggtctcgct ggccaagacg ggcgaaacgc ccgtgaccga attcctgcgc
      661 cagcgcttca acacttcact gaagattgtc gatggccatt tggcgggaca gccgtttctc
      721 atcgggaagc gccccaccat cgcggacttt tcgctgtgcg gttacatgtt ctttggggct
      781 gagcttccgc tgccgcttga gccctaccct cacgtcctaa gctggcttga acggatcaag
      841 tcactgccgg gatggaagca cccctacgat cttatgcctc gggaaccata gcttcgagat
      901 actcgtcgag gtgctcgagg ttctcaaagc gcatccagcc ctcatcggtt tcggcttcca
      961 cggtgccgtc ggagagcacc cgggccggtc ggccgcgaat gactttttcc tccaccacgt
     1021 aaagttcttc agcatcggcc gcttccggct ttgcttcggg ctcggctttg gcgacaacaa
     1081 attcttgcgg cgccgacttc tgcggctcga cgccgagggc cacggcaata tcggtctttg
     1141 cctgttccag tgcggccacc gtatcggcaa cgctggtttt ggaagcggcg gccgtttcgg
     1201 caacggcggc acttgcttca gccaccgccg cagtggccgc ggctttttta ccaaagccgg
     1261 tgaatttcgg ggtggcgggt ttctcttccg gcgtctcggg cgcagtttcc accacaacgg
     1321 gtgcaacggt ccggtcgcgc agcgccccta tggtgctgcc aagccccagg cacagggcgc
     1381 cgcccacgat gagaatagcc gcgacgtccg gggtgagacc ccgcacctgc attttaccgg
     1441 gtgaaaaatg gatgagaagt ccaagggcaa tacctgccgc caggaacaga ataccaaata
     1501 tgaacatcag tttcgacatc aaaaccccat agaccttatc gccggtcccc cctgtttccc
     1561 aagccccgct aggaatcccg cctcatggat aacacttcct gaaacgcctg cctagctttc
     1621 aaccgaaatc cgtggggcgg gcttggataa ctttccgcct tcgacggcgg cccacacctg
     1681 gcgggcgatg tccttgtaga ttgtggcatg aagcgagtcc ggcatggaga cgacgacggg
     1741 ttcgccgctg tcggagcgga tgcgcacttc cttgtcgagc ggaacttcgc cgaggaacgg
     1801 cacgcccagc cgggcggcct cttcgcgggc gccgccatgg ccaaaaattt catggcgctc
     1861 tccgcatttg ctgcagacaa aatagctcat gttctcgacg atgccgatca ccggcacatt
     1921 aaccttgcgg aacatgttga ggcctttgcg ggcgtcgatc agcgccaggt cctgcggagt
     1981 tgagacaatc acggcacctg acaatggtgt ctgctgggcg agcgtcagtt gggcatcgcc
     2041 ggtgccgggc ggcatgtcga cgatcatgac atcggtttcg ccccaggaca cttcgcgcag
     2101 a
//
LOCUS       DAORVN010000398         2101 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_55987_length_2101_cov_3.211632, whole genome shotgun sequence.
ACCESSION   DAORVN010000398 DAORVN010000000
VERSION     DAORVN010000398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55987_length_2101_cov_3.211632"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="PKV23_08185"
     CDS             <1..199
                     /locus_tag="PKV23_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013531143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sarcosine oxidase subunit beta"
                     /protein_id="HQX85452.1"
                     /translation="PVDGLYLNAGWCYGGFKATPASGWCFAHTIANDAPHKLNAAYRL
                     DRFRRGYMIDEKGQGASPNLH"
     gene            210..491
                     /locus_tag="PKV23_08190"
     CDS             210..491
                     /locus_tag="PKV23_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018328075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit delta"
                     /protein_id="HQX85453.1"
                     /translation="MLIKCPHCGTRPVEEFTFNGDASIRRPTSNDPKSMDQWFDYVYL
                     RDNPNGRFDEYAHHSSGCRAWLVVTRDTQTHEIFNVVTARDYAKKRVAP"
     gene            488..>2101
                     /locus_tag="PKV23_08195"
     CDS             488..>2101
                     /locus_tag="PKV23_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003548153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HQX85454.1"
                     /translation="MTGFRLDRGGLIDRTAPLDFTFDGRAFTGFAGDTLASALIANDV
                     HLMGRSFKYHRPRGVISAGSSEPNALVELREGPRKEANTRATMIELYDGLVARSQNRW
                     PSLEFDVGALNSLASAMFVAGFYYKTFMWPKSFWEKIYEPIIRRAAGLGHASKEADPD
                     KYEKAYAHCDLLVVGSGPAGLMAALTAARTGVHVILADEGSALGGSLLNENEEIGGMP
                     GHAWAMATIAELSAMPHVTLMPRTTIFGWYDDNIFGAVERVNDHVLEPSPYEPRQRYW
                     RIMAKRAVMATGAEERPIVFGGNDVPGVMTAGAMRTYANRYAAAAGKSVVVFTNNNSG
                     QRTAQHLRAHGIHVEAVIDPRKSGDVIVNVRGGKRVTAVELNGHRVIPCDAVAMSGGW
                     SPIVNLMCHRGAKPKWDERIAGFVPPDVGDKFVAAGSAAGKMLLSECLADGARAGTMK
                     GKTPAVPKCRDEAFAITPLWWVKESTGKAFVDYQNDSTAKDLPLAAQEGYRDIELAKR
                     YTTAGMATDQGKLGNVNAIAILAEATGRTM"
BASE COUNT          414 a          568 c          705 g          414 t
ORIGIN      
        1 gccggttgat gggctctatc tcaacgccgg ctggtgctat ggcggcttca aggcaacgcc
       61 ggcttcgggc tggtgttttg cccacacgat tgcgaacgat gcgccgcaca agctgaacgc
      121 ggcgtaccgg ctggaccggt tccgccgggg ctacatgatc gatgaaaagg ggcagggcgc
      181 ttcgcccaat ttgcattgat ggaaaagcca tgttgatcaa atgcccccat tgcggaacgc
      241 ggcccgtgga agagttcacc ttcaatggcg atgcttccat tcggaggcct acatccaacg
      301 accccaagtc gatggatcag tggtttgact atgtctatct gcgcgataat cccaatggcc
      361 gcttcgacga gtatgcgcat cattccagcg gctgccgggc ctggctggtg gtgacgcgcg
      421 atacgcagac gcatgaaatc tttaacgtgg tgacggcgcg ggactacgcg aagaagcggg
      481 tggcgccatg accgggtttc gtctggacag gggcgggctg attgaccgca cagcaccact
      541 tgatttcacc tttgacggca gggcgttcac cggatttgca ggggatacgc tggcctcggc
      601 gctgattgcc aatgacgtac atctcatggg gcgctcgttc aaatatcacc ggccaagggg
      661 cgtgatttcg gcgggcagtt ccgagcccaa tgcgttggtg gaactgcgcg aggggccacg
      721 caaggaagcg aacacgcggg ccactatgat cgagctttat gacgggttgg tggcgcggag
      781 ccagaaccgc tggccatccc ttgagtttga tgtcggcgcc ctgaactcgc tggcgtcggc
      841 catgttcgtg gcgggcttct attacaagac cttcatgtgg cccaagagtt tctgggaaaa
      901 gatctatgag ccgatcatcc gcagggcggc cggattggga catgcatcca aggaagcgga
      961 cccggacaag tatgaaaagg cctacgcgca ttgcgatctg ctggtggtgg gttccggtcc
     1021 tgccgggctg atggcggcgt tgacggcggc gcggacgggc gtccacgtta ttctcgccga
     1081 cgaaggttcg gcgctgggcg gttcgctgct caacgagaat gaagagattg gcggcatgcc
     1141 tggccatgcc tgggccatgg ctacaattgc cgaactcagc gccatgcccc atgtcaccct
     1201 catgccgcgc acgacgattt tcggctggta tgacgacaat attttcggcg cggtcgagcg
     1261 ggtgaatgac catgtgctgg agccttcgcc ctatgagcca cggcagcgct actggcgcat
     1321 catggccaag cgcgcggtga tggcgacggg agcggaagag cggcccatcg tgttcggcgg
     1381 caatgatgtg ccgggggtga tgacggctgg cgccatgcgg acctatgcca accgctatgc
     1441 ggcagcggcg ggcaaatcgg ttgtggtgtt caccaacaac aattccgggc aacgcactgc
     1501 gcagcatttg cgggcgcacg gcattcatgt ggaagcggtt atcgatccca ggaaatcagg
     1561 cgatgtcatc gtgaatgtca ggggcggcaa gcgtgtcacc gccgtggaac tcaacggtca
     1621 tcgggttatt ccctgtgatg cggttgccat gtcgggcggc tggagcccaa tcgtcaatct
     1681 gatgtgccat cgcggggcga aaccgaaatg ggatgagcgc attgccggct ttgtgccgcc
     1741 tgatgtcggg gacaagtttg tcgcggcagg atcggcggcg ggcaaaatgc tgctgtcgga
     1801 atgcctggct gacggggcac gggcgggtac aatgaagggc aagacgcctg ccgttccgaa
     1861 atgccgggat gaagcctttg ctatcacgcc gttgtggtgg gtaaaagagt ctacgggcaa
     1921 ggcttttgtc gattaccaga atgattcaac tgccaaggac ttgcctttgg cggcgcagga
     1981 gggctaccgc gacatcgaac tggccaagcg ctacaccacg gcgggaatgg cgacggatca
     2041 gggcaagctc ggcaatgtga atgccattgc catcctggcg gaagccacag gccggacgat
     2101 g
//
LOCUS       DAORVN010000399         2100 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_56050_length_2100_cov_1.756968, whole genome shotgun sequence.
ACCESSION   DAORVN010000399 DAORVN010000000
VERSION     DAORVN010000399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2100
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56050_length_2100_cov_1.756968"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            48..1505
                     /locus_tag="PKV23_08200"
     CDS             48..1505
                     /locus_tag="PKV23_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008547745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HQX85455.1"
                     /translation="MTTQDIVQKLWNLCDVLRDDGINYSDYVTELVLMLFIKMEYENT
                     ESGILQAHKLPPYARWPELRDKSGLNLLRHYRETLLKLSASDDRLIAAIYADAQTSLK
                     EPRHLEQLVKSLDGIDWFSARQDGLGDLYEGLLEKNASETKSGAGQYFTPRPLIDAIV
                     QVIKPQPGETVQDPAAGTAGFLIAADRYIKDHTDDLYNLSDRERRFQRNQAFLGMELV
                     PSTRRLALMNCLLHGMEGDDEGVVHVGNTLGSAGSSLPKSHIILSNPPFGTAKGGGGP
                     TRDDLTFATNNKQLAFVQHIVRHLRDGGRAAVVLPDNVLFEAGIGADIRRDLMDKCRL
                     HTILRLPTGIFYAQGVKTNVLFFEKVGQAATGSTQEVWVYDMRANAPKFGKRTPLEDK
                     HFANFIAAYGDEPNGKAARKDQGELGRFRRFSRDWIADEKGDSLDIAWLKDGSDEGVA
                     DLPEPAVLAREAAAELTGALTELDAILKELGEVAE"
     gene            1502..>2100
                     /locus_tag="PKV23_08205"
     CDS             1502..>2100
                     /locus_tag="PKV23_08205"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HQX85456.1"
                     /translation="MRALPAGWATASIADIALFNPKTVAEDSMNAGFAPMQLLGTRYR
                     DRMVFEERPWGEIKKAYVHFQQGDVIVAKVTPCFENGKAGIAPPLPNGLGAGSSEFCV
                     FRPAQGIDERYLLAWLSTEDFRRRATVAMTGSVGLKRVPKEVFLSERIPLAPESEQKR
                     IADKLDSLLARVDACRERLDRIPAILKRFRQSILAAACSG"
BASE COUNT          463 a          653 c          610 g          374 t
ORIGIN      
        1 cctgacaatt aagtgtttta cgctcaacaa attcacaaaa ctcaaatatg acaacccaag
       61 acatcgtcca gaagctctgg aacctctgcg acgtcctgcg cgacgacggc atcaactaca
      121 gcgattacgt caccgagctc gtgctgatgc tcttcatcaa gatggagtac gagaacaccg
      181 aaagcggcat cctccaggcg cacaagctgc cgccctatgc ccgctggccc gagttgcgcg
      241 acaagagcgg cctgaatctg ctgcgccatt accgtgaaac cctgctcaag ctctcggcca
      301 gcgacgaccg gctgattgcc gccatttacg ccgacgcgca gaccagcctg aaagaaccgc
      361 gccacctgga gcagctcgtc aaaagcctgg atggcatcga ctggttcagc gcccggcaag
      421 acggcctcgg cgacctgtac gaaggcctgc tggaaaagaa cgccagcgag accaagagcg
      481 gcgccggcca gtacttcacg ccccgccccc tgattgacgc catcgtgcag gtcatcaaac
      541 cgcagccggg cgagaccgtg caagacccgg cagcgggcac ggctggcttc ctgattgccg
      601 cggaccgcta tatcaaggac cacaccgacg atctttacaa cctcagcgac agagaacgca
      661 ggtttcagcg caaccaggcc ttcctgggca tggagctggt acccagcacc cgccgcctgg
      721 cgctgatgaa ctgtctactg cacggcatgg aaggcgacga tgaaggagtc gtgcatgtcg
      781 gcaacacgtt gggcagtgcc ggcagcagcc tgcccaagag ccacatcatc ctcagcaacc
      841 cgcctttcgg caccgccaaa ggcggtggcg gcccaacccg cgacgacctg acctttgcca
      901 ccaacaacaa acagctcgcc tttgtgcagc acatcgttcg gcacctacgc gatggagggc
      961 gtgccgctgt agtgctcccc gacaatgtgc tgtttgaggc cggcataggc gccgacatcc
     1021 gccgcgacct gatggacaag tgccgattgc acaccatcct gcgcttgccc actggcatct
     1081 tctacgccca gggcgtcaag accaacgtgc tgttcttcga gaaggtcggc caggccgcca
     1141 ccggcagcac gcaagaagtc tgggtgtacg acatgcgggc caacgcgcca aagttcggca
     1201 aacgcactcc gctagaagac aagcactttg ccaacttcat cgctgcgtat ggtgatgaac
     1261 caaacggcaa ggccgcgcgg aaggaccagg gcgaattggg acgctttcgc cgcttcagcc
     1321 gcgattggat cgccgacgaa aaaggcgaca gtctggacat cgcctggcta aaggatggca
     1381 gtgacgaagg cgttgccgat ctacctgaac cggctgtgct ggcgcgcgag gcggcagccg
     1441 aacttacagg ggcactaacg gagctggacg ctatcctgaa ggaattgggc gaggtggcgg
     1501 aatgagagcg ttgcctgccg gctgggctac tgcctctatc gcggacattg cactcttcaa
     1561 ccccaagacg gttgctgaag actcaatgaa tgctggcttc gcaccaatgc agttgttggg
     1621 tacgcgatac cgggatcgca tggtctttga agagcggcct tggggtgaga taaagaaggc
     1681 ctacgtgcat ttccaacaag gtgatgtgat tgttgccaaa gtcactccct gttttgagaa
     1741 cggaaaggct ggtatcgcgc ccccgttgcc taacggtctt ggtgcaggca gttccgagtt
     1801 ctgtgtcttt cggccagccc aaggcattga cgagcgttac ttgttggcat ggttaagcac
     1861 ggaggacttt cgacgacgcg cgacagttgc gatgacgggc tccgtggggt tgaagcgggt
     1921 gccgaaagag gtgtttctat cagagcggat accgttggcg cctgagtccg agcaaaaacg
     1981 catcgccgac aagctggatt cccttctggc ccgagtcgat gcctgtcgcg aacgcctcga
     2041 ccgcatcccc gccatcctga aacgcttccg ccaatccatc ctcgccgccg cctgctccgg
//
LOCUS       DAORVN010000400         2095 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_56255_length_2095_cov_3.373039, whole genome shotgun sequence.
ACCESSION   DAORVN010000400 DAORVN010000000
VERSION     DAORVN010000400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56255_length_2095_cov_3.373039"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1076)
                     /locus_tag="PKV23_08210"
     CDS             complement(<1..1076)
                     /locus_tag="PKV23_08210"
                     /inference="COORDINATES: protein motif:HMM:NF014919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching enzyme GlgX"
                     /protein_id="HQX85457.1"
                     /translation="MPHSHSGSPFPPGVTPVEGGVNVAVVSRHAARIFFCLFEGSMET
                     RFELASRQGDVHFGFIPGVKAGQHYGLRAEGPYDSANLFDVSKLLIDPYATAIDRSFT
                     WHEDLAIKGAESAHLVPHCIVAAALPTVQHLPHAAPRFIYEVAVKAFTRLHPQIPENI
                     RGTVAALAHPACIEHFLKLGVDTIELMPLMAWADERHLARQGLSNAWGYNPISFFAPD
                     PRLAPAGLSEIRDTIAALHGAGLGVVLDVVFNHTGESDLGGPTVSLRGLDNALYYRHA
                     KGVLVNDTGCGNTLDTNSPPVMDLILAAMRHWVTATGLDGFRYDLATVLGRGPHGFEA
                     RAPLLEAIQQDALLGPLIHIAEPW"
     gene            complement(1078..1974)
                     /locus_tag="PKV23_08215"
                     /pseudo
     CDS             complement(1078..1974)
                     /locus_tag="PKV23_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010970236.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
BASE COUNT          434 a          660 c          628 g          373 t
ORIGIN      
        1 tcccagggct cggcaatatg aatgagcggg cccagcagtg catcctgctg aatggcctcg
       61 agcaggggag cccttgcttc aaagccatgg ggcccgcgcc ccagcaccgt ggcaagatca
      121 tagcgaaacc cgtcaagccc ggtcgccgtc acccaatggc gcatggcagc cagtatgaga
      181 tccatgaccg gtggactgtt tgtgtccaag gtattgccgc agcccgtgtc gttcaccagc
      241 acgcctttgg catggcggta gtaaagtgcg ttgtcgaggc cgcggagcga aaccgtcggc
      301 ccgcccaggt cgctctcacc cgtgtgattg aacaccacat cgaggacaac gcccagcccc
      361 gcgccatgca gcgccgcaat ggtatcgcgg atttccgata gtccggccgg cgccagccgc
      421 gggtccgggg cgaagaagct gatggggttg tagccccagg cattcgaaag cccctgccgc
      481 gccaggtgcc gttcgtcggc ccaggccatc agcggcatca gctcaatcgt atcaacgccc
      541 agcttcagaa aatgttcgat gcaggcggga tgcgccaggg ccgcaacggt gccgcggatg
      601 ttctcgggaa tctgcggatg cagccgggtg aaggctttca ccgccacttc ataaatgaag
      661 cggggcgcag cgtggggcaa atgctgcact gtcggcaatg ctgccgcaac gatgcaatgg
      721 ggaacgagat gcgctgattc agcgcccttg atggccagat cctcatgcca ggtaaagctc
      781 cggtcaatcg ccgtggcata ggggtcgatc agcagcttgg aaacatcgaa aagattggca
      841 gagtcgtaag ggccttccgc ccgcaagccg tagtgctggc cagcctttac gccaggaatg
      901 aagccgaaat gcacatcgcc ctggcgggac gccagttcaa accgtgtttc catgctgcct
      961 tcgaacaggc agaaaaatat ccgcgccgca tgtcgagaaa caacggcaac attgaccccg
     1021 ccttcaacgg gagtgacgcc gggcggaaac ggcgagccgg aatgcgaatg aggcatatca
     1081 ggtaatcaca gtcggttccg tgcggcccgt gcgctccgca atgccggcaa ggccccgcgc
     1141 aaacccgatg agatcggaaa gtgccgcctg cgtttcaagc ccgaggactt tggcctcgta
     1201 tttctcgata tagacgcgga gcgtggctcc cgccgtgccc gtgccggaaa gccggtagat
     1261 cacccgcgaa ccgtcgtcga aaataatgcg gatgccctgg tgggcagtgt cggaaccgtc
     1321 gacgatgtca tggtaggaaa aatcatcggc cgtcgccacc gccagcgccc ccgctttttc
     1381 acccttgagg ctgggcagct tgtcacgcag gaatgtcatc agcctgtttg ccgcatccag
     1441 atccacttcc tcgtaatcat ggcgcgaata atagttgcgc ccgtattccg cccaatgctg
     1501 cctgatgatg tccgccatgc tttgccggcg cctggccagg atattgagcc acagcagcac
     1561 agcccagagg ccatccttct cgcgcacatg gtttgatccc gttcccgcac tttcctcacc
     1621 gcacatggtg atcaacccgg catcgagaag attgccaaag aacttccacc ccgtcggcgt
     1681 ttcgaagcat ttgattccca gcttggcggc aaccctgtcc accgcgcagc tcgtgggcat
     1741 ggaacgcgcc acacccgcaa gacccttggc atagcccggg gccagatggg cattggcagc
     1801 cagcagcgcc agtgaatcgg atggtgttac aaactggttc ttgccgatga ttaggttgcg
     1861 gtcgccatcg ccatcggacg cggcgccgaa atcaggaccg cctgccgaca tcatcagatc
     1921 ataaagttcc ttcgcgtgaa ccagattggg gtccggatga tgcccgccga aatccaggcg
     1981 agccgccagg tcgaggtgga cgagccggcg tacgagccgc cccatcgctc accacggccg
     2041 agcagcgccc ctccgcgtga gtcgagcggc ggtctcgact tcaagatccg agtgg
//
LOCUS       DAORVN010000401         2093 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_56366_length_2093_cov_3.468597, whole genome shotgun sequence.
ACCESSION   DAORVN010000401 DAORVN010000000
VERSION     DAORVN010000401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2093
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56366_length_2093_cov_3.468597"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            142..1116
                     /locus_tag="PKV23_08220"
     CDS             142..1116
                     /locus_tag="PKV23_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020179598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HQX85458.1"
                     /translation="MAGVAPQKSGPVPQELDLPGGKSGFRNWLDRNAWRDIIIAVPYI
                     WLILFFVAPFIIIIAMSLAQSVIAQPPFRFYDTWPYVTFENFERLFSDYVYLRGYLTS
                     LRNALIATALCLLLGYPMALGIARAKGVWRNILLLLVILPFWTSFLLRVYAWIGMMGS
                     NSWFNRGLTYTINAFLPAAWEINFVPMMNSNFAVILVVVYSYLPFMILPLFANLEKLD
                     FTLNEAAMDLGSRPMQVFRDVTLPLSIPGIVAGCLLVFIPATGELVIPSLVGNAGDPM
                     IGRVINDEFSLNRHWPMASTIAVALLMLLVVPIMLYNRMQERAAEGAK"
     gene            1116..1955
                     /locus_tag="PKV23_08225"
     CDS             1116..1955
                     /locus_tag="PKV23_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018234765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HQX85459.1"
                     /translation="MRQRSTFLFSVLCFGFAFFYIPIISMIVYSFNKSRLATVWGGFS
                     VEWYFKLFQNAQIIKAATLSLEIAVISATFATILGTMAGMALARFSKFRGRTLFSGLV
                     SAPLIMPEVITGVSLLLLFVFMARYVGWPAERGFTTVTIAHTTFALSYVAVIIQSRLL
                     SMDQSLEEAAMDLGSKPWQVLFDVTLPIIAPAMISGWLLAFTISLDDVVITNFTTGPG
                     ATTLPILIWGKVKLGVTPDINALATIIVLVVSVGVGLAGYLMYRSEKKRDRDIQMALA
                     ANK"
BASE COUNT          362 a          653 c          555 g          523 t
ORIGIN      
        1 aaacttcctt cctcggcgct ggtccgcgtc aaccgggtga atgaccatcg cggccacgaa
       61 accgaacggg tcgcccaatg ggaagacaag gtatggcttt cctttgagcc gtcagccgca
      121 attctgttga aggattaggc catggccggc gtcgctccgc agaaatccgg gcccgtaccg
      181 caggagcttg acctgcccgg cggcaagtct ggctttcgca actggcttga ccgcaacgcc
      241 tggcgcgaca tcatcatcgc cgtgccctat atctggctga tcctgttttt tgtggcaccc
      301 ttcatcatca tcattgccat gagcctggcg caatcggtga tcgcccagcc gcccttcagg
      361 ttttatgaca cctggcccta tgtcacgttt gagaatttcg agcggctctt ttccgattat
      421 gtgtatctgc gcggctatct cacctcgctc cgcaacgcgc tgattgcaac cgcactctgc
      481 ctgcttctcg gctatccaat ggccctggga attgcgcgcg ccaagggcgt ctggcgaaat
      541 atcctgctcc ttctcgtcat cctgccgttc tggacttcgt tcctgctgcg cgtctatgcc
      601 tggatcggca tgatgggaag caacagctgg ttcaaccggg ggcttaccta tactatcaac
      661 gcgttcctgc ccgcggcctg ggaaatcaat ttcgtcccga tgatgaattc gaacttcgcc
      721 gtaattcttg tcgtggtcta ttcctatctg cccttcatga tcctgccgct cttcgcaaac
      781 ctggaaaaac tcgacttcac cctgaacgag gcggcgatgg atctgggatc ccggcccatg
      841 caggttttcc gcgacgtgac gctgccgctc tccattccgg gaattgtcgc cggctgcctc
      901 ctggttttca tcccggcaac gggcgagctg gtcattccaa gtctcgtggg caatgccggc
      961 gacccgatga ttggccgcgt gatcaatgac gagttctcgc tcaaccgcca ctggcccatg
     1021 gcttccacca ttgccgtggc cctgctcatg ctcctcgtcg tgccgatcat gctttacaac
     1081 aggatgcagg agagagccgc cgagggagca aagtaatgcg ccagcgctcc acattcctct
     1141 tttcggtcct gtgtttcggc tttgccttct tctacatccc gatcatctcg atgattgtat
     1201 attccttcaa caaatcacgg cttgcgacgg tctggggcgg cttctcggtt gagtggtatt
     1261 tcaagctgtt ccagaatgcc cagattatca aggcggcaac cctctcgctt gaaattgctg
     1321 tcatcagcgc cacctttgca acgatcctcg gcaccatggc cggcatggcc ctggcgcgat
     1381 tctcaaaatt ccggggccgc acgctctttt ccgggcttgt gtccgcgccc ctcatcatgc
     1441 cggaagtcat taccggtgtt tccctgctcc tgctgttcgt gttcatggcg cgctatgtcg
     1501 gctggccggc cgagcgcggc tttaccaccg tcaccatcgc ccacacgacc tttgcgcttt
     1561 cctatgtggc agtgatcatt caatcgcggt tgctgtcgat ggatcagtcg ctggaggaag
     1621 ccgccatgga cctgggctca aagccctggc aggtgctgtt tgacgtgacc ctgccgatca
     1681 tcgcacccgc catgatatcc ggctggctcc tggctttcac catttcactg gatgacgtgg
     1741 tcatcaccaa cttcaccacc ggcccggggg ctacgactct gccaatcctg atctggggca
     1801 aggtgaagct gggcgtgacg cccgatatca acgcgctggc aacgattatt gtccttgtgg
     1861 tcagtgtcgg tgtcgggctt gccggttatc tcatgtaccg cagcgagaaa aagcgcgatc
     1921 gcgatatcca gatggccctt gccgccaaca agtaggatta gtcgtggctg ctgacgagtt
     1981 tcggttttgc ctcggccagc tttgccggct cttccacctc aaggctttcg atgcgaagcc
     2041 cgcgcttgac gattttttcc gccgagagtg aaagcttttc cagatccgaa ttg
//
LOCUS       DAORVN010000402         2093 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_56387_length_2093_cov_2.610893, whole genome shotgun sequence.
ACCESSION   DAORVN010000402 DAORVN010000000
VERSION     DAORVN010000402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2093
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56387_length_2093_cov_2.610893"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..707)
                     /locus_tag="PKV23_08230"
     CDS             complement(<1..707)
                     /locus_tag="PKV23_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012110968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HQX85460.1"
                     /translation="MTSRILIVEDDPAQRRILEELVKRFGFDSIAADGGIKGLEVLNG
                     PNGGSIELVILDLMMPGMDGLEFIEKMQGIRGDLPVIVQTAQGSIETVIKAMRAGADD
                     FVVKPVSPERLKISIQNLLKVNALTEEVKRLNKKVGGSLSFSDLIASSPAMANVLRLG
                     RRGAQSNIPILIEGESGVGKEMIARAIQGESDRAGRPFVAVNCGAIPENLVESILFGH
                     EKGSFTGATSKHNGKFQ"
     gene            915..>2093
                     /locus_tag="PKV23_08235"
     CDS             915..>2093
                     /locus_tag="PKV23_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007877131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HQX85461.1"
                     /translation="MDMTASAALGNLPEWNLADLYAAPDAAAFKADMLKGEAEAKAFA
                     AKYRGKLAKLSGAELAVALKSYEAISDLLGRTGSYAQLYYVGDTTDAARGKFYGDVSS
                     KHTEISTQLLFFELELNRIDDAALEKAMQDPGLNHYRPWIENLRKERPYQLDDKLEEL
                     FLEKSQTSYSAFNRLFDETMADLRFEVDGESLAIEPTLNLLQSPDEAKRKAGALALAK
                     TFQENLRLFTLITNTLAKDKDISDRWRGFKDIAAARHLSNRVEPEVVDALVAAVRAAY
                     PKLSHRYYKMKAKWLGKDRLMHWDRNAPLPAEDTREVPWAEAEKMVLSAYGEFSPEMA
                     AIAKTFFDKKWIDAPVRPGKAPGAFAHPTVPSAHPYVLLNYMGKTRDVMTLAHELGHG
                     V"
BASE COUNT          453 a          585 c          627 g          428 t
ORIGIN      
        1 tcctgaaatt tgccgttatg tttgctggta gcgccggtaa aggaaccctt ttcgtggccg
       61 aagagaatgg actcaacaag gttttccgga atggcgccgc agttgaccgc cacaaagggg
      121 cggccggcgc gatcgctttc gccctggatg gcgcgggcaa tcatttcctt gccgacgccc
      181 gattcgcctt cgatcaggat ggggatgttg gattgggcgc cgcggcggcc gaggcgcagg
      241 acattggcca tggccggaga agacgcgata aggtcgctga aactcaggga gccgccgact
      301 ttcttgttca ggcgcttcac ttcctcggtg agcgcattga ccttcagcag attctgaatg
      361 gaaattttca ggcgctcggg cgatacgggc ttcaccacaa agtcgtcggc accggcgcgc
      421 atggccttga tcacggtttc gatagagcct tgcgcggttt gcacgatgac gggcaggtcg
      481 ccgcgaatac cttgcatttt ctcgatgaat tccagcccgt ccattccggg catcatgaga
      541 tcgaggatca ccagctcgat gctgccgcca tttggcccgt tcaagacttc cagtcccttg
      601 attccgccat ctgctgctat tgaatcaaaa ccgaaccgct tgacaagttc ttccagaatg
      661 cggcgttggg cgggatcgtc ttcgacaatc agtatgcgtg aagtcatgct gtttaatcaa
      721 ctcatcgtca aagggccaaa ttcaggtgcg gccaagcaac aggcaccccc cgtctgcttt
      781 gctttccctg ttgcccgtag tctcgcagtg aagggtaaag gccctgttaa tgagatagat
      841 tatttgggga caaattcagg cagttgccga agccgcgggc atgagccata tgggaaacaa
      901 attgttaaca aggaatggac atgaccgctt ccgccgcact cggaaatttg cccgaatgga
      961 acctcgctga cctctatgcc gcgcccgatg cggcggcctt caaggccgac atgctgaagg
     1021 gcgaggcgga agccaaagcc ttcgccgcaa agtatagggg gaaactggca aagctctcgg
     1081 gcgcggagct tgccgtggcg ctcaaaagct atgaggccat ttcagatctg cttggccgca
     1141 ccggctccta tgcccagttg tattatgtag gggatacaac ggatgcggcg cgcggcaagt
     1201 tttatggcga cgtgagttcc aagcacacgg aaatttcaac tcagctcctg ttctttgaac
     1261 ttgagctcaa ccggattgac gatgcagccc tcgaaaaggc catgcaggac ccggggctca
     1321 accactaccg gccatggatt gagaacctgc gcaaggagcg gccctatcag cttgatgaca
     1381 agctggagga gctgttcctc gaaaagtccc agacgagcta cagcgctttc aaccgcctgt
     1441 tcgatgaaac catggcggac ctgcgcttcg aggtggacgg ggagtcgctg gcaattgaac
     1501 ccaccctcaa tctcttgcaa agcccggatg aggccaagcg gaaggcgggg gccctggcgc
     1561 tggccaaaac cttccaggag aatttgcgtc tcttcaccct gatcaccaat acgctggcaa
     1621 aggacaagga catttccgac cgctggcggg gtttcaagga cattgccgcg gcgcgtcatc
     1681 tttcaaaccg ggttgaaccg gaagtggtgg atgcgctggt ggcggcggtg cgcgccgcat
     1741 acccaaagct ttcgcaccgc tattacaaga tgaaggcgaa gtggcttggc aaggacaggc
     1801 tgatgcattg ggaccgcaat gcgccattgc cggcggaaga cacgcgcgaa gtgccctggg
     1861 cggaggcgga gaaaatggtg ctctcggctt acggcgaatt ctcgccggag atggcggcca
     1921 tcgccaaaac ctttttcgac aagaagtgga ttgatgcgcc ggtgcggccc ggcaaggcgc
     1981 ccggggcatt cgcacatccc accgttccat cggcgcaccc ctacgtgctc ctcaattaca
     2041 tgggcaagac gcgggacgtg atgaccttgg cccacgaatt ggggcatggc gtg
//
LOCUS       DAORVN010000403         2091 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_56476_length_2091_cov_3.606582, whole genome shotgun sequence.
ACCESSION   DAORVN010000403 DAORVN010000000
VERSION     DAORVN010000403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2091
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56476_length_2091_cov_3.606582"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1093)
                     /locus_tag="PKV23_08240"
     CDS             complement(<1..1093)
                     /locus_tag="PKV23_08240"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018098967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HQX85462.1"
                     /translation="MMTCCPVGPALDAASQFAGDLAPWVITRPDGDRELDLIIPGLCA
                     PESIPLIEGALEQVPGLAAARVNFTGKRVIATWTDPAFNPAGIVEALNELGFMSRPFD
                     PSHSGLDADDAQSRLLLRAVAVSGFAAANIMLLSVSVWSGAEGSTRDLFHWISAGIAL
                     PTVAYAGRPFFRSAVRALSSGQFNMDVPISLAVCLAALMSLYETFNHGEVAYFDASVT
                     LLFFLLTGRYLDHLMRARARSAVSQLLSLSASSAMVEEADGSTRIMAATALQPEMKML
                     VAAGERLAADGVVAAGSSEIDRSLLTGESNPEVAAKGAIVHAGMLNLTGPLTVTVTAA
                     GQDTFLAEIIRLMASAEQSRSRFVRVADRL"
     gene            complement(1104..1568)
                     /locus_tag="PKV23_08245"
     CDS             complement(1104..1568)
                     /locus_tag="PKV23_08245"
                     /inference="COORDINATES: protein motif:HMM:NF017559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HQX85463.1"
                     /translation="MTRPFTGRHMAIIIISFFAVIIAVNLTMAYFARSSWTGLVVKNS
                     YVASQSFNRDAEIARQQQALGWQMTLKVKREAVQFTVLDRDNQAMTGLRIRAVLQRPT
                     DEAGDQHLKLRESGAGIYRTDAVIGGGVWVADITAEKSDTELVRFVQRIFVK"
     gene            complement(1565..>2091)
                     /locus_tag="PKV23_08250"
     CDS             complement(1565..>2091)
                     /locus_tag="PKV23_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002717188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FixG Ig-like domain-containing protein"
                     /protein_id="HQX85464.1"
                     /translation="TLKTYDAHKAGGLAVIGWRAFLRPRTFVYSAIWLAVGLAMFYSL
                     LHRERLEVNVLNDRNPIYVRLSDGSIRNGFTVKILNMEQRPRSFVVSAEGLPGSSMTM
                     TGGDGAPLREFTIEVEADKLRAIKVYVAVPPLLLEGEQVPFEFKVVEQASQTTAETRS
                     IPAIFHAPPKETQP"
BASE COUNT          394 a          674 c          668 g          355 t
ORIGIN      
        1 ccagccggtc ggcaacccgg acaaagcgcg agcggctctg ctcggccgag gccatgaggc
       61 ggatgatttc cgcaagaaat gtatcctgcc cggcggcggt gaccgtgacg gtgagtggcc
      121 ccgtgagatt gagcatgccg gcatgaacga tggcgccttt ggccgccact tccggattgc
      181 tctcgccggt cagcagcgac cggtcgattt cactgctgcc tgcggcgacg acgccatcgg
      241 cggccaggcg ctcgcccgcc gcgaccagca ttttcatctc cggctggagc gccgttgcgg
      301 ccatgatgcg ggtgctgccg tcggcctctt cgaccatggc gctggaggcc gaaagcgaca
      361 ggagctgcga cacggcggag cgcgccctgg cccgcatgag gtgatcgagg tagcggccgg
      421 tgagcaggaa gaagagcagc gtcaccgacg cgtcgaaata ggcaacctcg ccgtggttga
      481 aggtttcata gaggctcatc agcgccgcga ggcaaaccgc cagcgagatc ggcacatcca
      541 tgttgaactg gccggacgac agggcgcgca ccgcgctccg gaaaaacggc cgccccgcat
      601 aggcgaccgt gggcagcgca atgccggcgg aaatccagtg gaacaggtca cgggtgctgc
      661 cctccgcccc ggaccagacg gaaaccgaca gcagcatgat gttggcggcg gcaaagcccg
      721 acacggccac ggcgcgcagc agcagccggc tttgcgcgtc atcggcatcc agccccgaat
      781 ggctgggatc aaagggccgg gacatgaagc caagctcatt caacgcctcg acgatgccgg
      841 caggattgaa ggcgggatca gtccacgtgg cgataacccg cttgccggtg aagttcaccc
      901 gcgccgcggc aaggcccgga acctgttcca gcgcgccctc gataagcgga atgctctcgg
      961 gcgcacagag tccgggaatg atcaggtcaa gttcccggtc accatcagga cgcgtgatga
     1021 cccacggcgc gagatcgccg gcgaactggc tggcggcatc aagggctggg cccaccgggc
     1081 agcaggtcat catcgcaatc gtcctacttc acgaagatgc gctgcacgaa gcgcaccagt
     1141 tccgtgtccg acttttccgc cgtgatatcg gcaacccaga ccccgccgcc aatgaccgca
     1201 tcggttctgt atatgccggc tccgctctcc cgcagcttga gatgctggtc gcccgcctca
     1261 tccgtcggcc gctgcagcac ggctctgatg cggagccccg tcatggcctg gttatcccgg
     1321 tcaaggacgg tgaattgcac cgcctcacgc ttcaccttga gcgtcatctg ccagcccagc
     1381 gcctgctgct gcctggcgat ttccgcatcc cggttgaagc tctggctcgc cacatagctg
     1441 tttttcacca ccagtccggt ccagctcgat ctggcgaaat acgccatggt caggttgacc
     1501 gcgatgatga ccgcaaagaa tgaaatgatg atgatggcca tgtgcctgcc ggtgaagggg
     1561 cgcgtcatgg ttgtgtctcc tttggcggcg cgtggaagat ggccgggatg ctgcgcgttt
     1621 ccgctgtggt ctggctcgcc tgttccacaa ccttgaattc gaagggcacc tgctcgccct
     1681 ccagcagaag gggcgggacg gcaacataga ccttgatcgc ccgcagcttg tcggcctcaa
     1741 cctcaatggt gaattcgcgc aagggcgcgc catcgccacc ggtcattgtc atgctgctgc
     1801 ccggaagccc ttcggcgctg acaacaaagc tgcgcggccg ctgctccatg ttcaggatct
     1861 tgaccgtgaa gccgttgcgg atgctgccat ccgaaagccg cacatagata gggttgcggt
     1921 cgttgagaac attgacctca agccgttcgc ggtgcagcag cgaatagaac atggccagcc
     1981 ccaccgccag ccagatggcg ctgtagacaa aggtccgggg ccgcaggaag gcgcgccagc
     2041 cgatgactgc caggccgccc gccttgtggg catcataggt tttgagcgtc g
//
LOCUS       DAORVN010000404         2082 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_56900_length_2082_cov_2.667982, whole genome shotgun sequence.
ACCESSION   DAORVN010000404 DAORVN010000000
VERSION     DAORVN010000404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2082
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56900_length_2082_cov_2.667982"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..646)
                     /locus_tag="PKV23_08255"
     CDS             complement(<1..646)
                     /locus_tag="PKV23_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002964780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HQX85465.1"
                     /translation="MYQFRNFAVWIVIALLLFALFSLFQGQVGGTTNQEISYSQFFDR
                     VNSGEIKSVTYSGESISGKLSDGKSVQAYGIFTEEDLKSMRQKGVEVSFRPQQTDSLL
                     SNALIYWLPMLLLVGVWVFFIRQMQSGSGKAMGFGKSKAKLLTEKQGRVTFEDVAGVD
                     EAKEDLVEIVDFLKDPHKFQRLGGKIPKGALLVGPPGTGKTLLARAIAGEANVPF"
     gene            complement(750..1988)
                     /gene="tilS"
                     /locus_tag="PKV23_08260"
     CDS             complement(750..1988)
                     /gene="tilS"
                     /locus_tag="PKV23_08260"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HQX85466.1"
                     /translation="MRAAEAEQRTYSKQDCDELFAALRGEDHFAVAVSGGSDSMALLR
                     LALQWANGSARISVLTLDHGLRPGAAAEAAKVAEWCTLLGVEHHTLRWEGTKPETGLQ
                     AKARSARYDLMCNWCKANQVKFLLTGHTMDDQAETVLMRQARTDTAESLAGIWQTADW
                     KGVRLFRPLLGQSRADLRGYLTGLGQPWIDDPSNLDRRFERVRARQALAQGERPELQK
                     IELADIAEKAGRAARGLALATDKWIRGQLTSYPEGFGAIPRAGFCELEPSLQRRVLQQ
                     LILIYGAGNRAEPGELDYLAKWIMGQGVSRRTLGGAMLACRQSSVLIGREWARIAADA
                     TIVPESGEIVWDGRFLIKAPSKSQVVAVGRLNGIVRREDIPSFVQQSLPAVILGGGNT
                     VVPHLAAGDGVSAKFIRHLR"
BASE COUNT          420 a          686 c          549 g          427 t
ORIGIN      
        1 agaacggcac attggcttcg cccgcgatgg cgcgcgccaa aagtgtcttg cccgtgcccg
       61 gaggtcccac aagcagcgcg cctttcggaa tcttgccgcc aagccgctgg aacttgtgag
      121 ggtccttgag gaagtcaacg atttccacca ggtcttcctt ggcctcgtcg acgccggcca
      181 cgtcttcaaa ggtcacgcgg ccctgctttt cggtcagcag cttcgccttt gatttgccaa
      241 agcccatggc cttgccggag cctgattgca tttgccggat gaaaaacacc cagacaccga
      301 ccagcagcag catgggcagc caatagatca gcgcgttgct gagcaatgaa tccgtctgct
      361 gcgggcggaa gctgacttca acccccttct gccgcatgct cttgagatct tcttcggtaa
      421 agatgccgta ggcctgcacc gatttgccgt cagacagctt gccggaaatc gattcgcccg
      481 aataggtcac cgatttgatt tcgccggaat tgacccggtc aaaaaactgc gagtagctga
      541 tttcctggtt ggtggttccc cccacctggc cttggaacag gctgaacaat gcaaacagca
      601 ggagcgctat gacgatccag acggcaaaat tacggaactg atacaattaa ccctcaaatc
      661 tcccgtgctg gccaaaatgg ccgccagagc aatcaaccca atcttcaact gtaacatagg
      721 atgggatgag accaattcca agcatggaac tatcgtaaat ggcgtataaa tttggccgaa
      781 accccgtctc ccgccgccaa atggggaacc acggtatttc cacctcccag gataaccgcc
      841 ggtaggctct gctgcacgaa acttggaatg tcctcccgcc gcacaatgcc gttcaggcgc
      901 cccaccgcaa ccacttggga ttttgacggc gccttgatca ggaaccgccc gtcccacacg
      961 atttctcctg attcagggac aatcgtggca tcggctgcaa tccgcgccca ttcacgcccg
     1021 atcagcacgc ttgactgtcg gcaggcaagc atcgctccac ccagagttcg ccgtgaaact
     1081 ccctgcccca tgatccactt cgccaggtaa tcgagctcgc cgggttcggc ccggttgccc
     1141 gctccgtaaa tcagaatcag ctgctgcagg acccgccttt gcaggctggg ctcgagttcg
     1201 caaaatcccg cccgcgggat ggcgccaaag ccttccggat aggaagtgag ttgccccctg
     1261 atccacttgt cggtggcaag ggcgagcccc cgcgcggcac gccctgcctt ttcggcaata
     1321 tctgccagtt cgattttttg aagctcgggc ctctcccctt gcgccagtgc ctggcgcgcc
     1381 cgcacgcgct caaaccgcct gtccaggttg ctgggatcat caatccatgg ttgcccgagg
     1441 cccgtcagat agccgcgcaa atcagcccgg ctttgcccta gcagcggccg gaagagcctc
     1501 acgcctttcc agtcggcggt ctgccagatg ccggccaggc tttccgccgt atcggtgcgg
     1561 gcctgccgca tcaacacggt ttccgcctgg tcatccatcg tatggcccgt gagcaggaac
     1621 ttgacctgat ttgccttgca ccagttgcac atcaagtcgt aacgggcact tctggctttt
     1681 gcctgcaggc ccgtctcggg ctttgtccct tcccaccgca acgtatgatg ctcaactccg
     1741 agaagagtgc accattcggc aactttggct gcctcggccg cggctcccgg ccgcaggccg
     1801 tgatcaagcg taagtaccga aatcctcgca gatccatttg cccattggag agccaggcgc
     1861 agcagcgcca tcgaatccga accgcctgag acagctacgg caaaatgatc ctcgccgcga
     1921 agcgccgcaa aaagttcatc gcaatcctgt ttgctgtaag tgcgctgctc tgcctcagca
     1981 gcccgcacgc ttggattccg cttgcgcccg cttcttggca ttgaccgcat tgggaaactc
     2041 gccgccaagc gcgccaaatg ccgcgcaggc ctgctgtgtc tg
//
LOCUS       DAORVN010000405         2080 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_56983_length_2080_cov_2.819753, whole genome shotgun sequence.
ACCESSION   DAORVN010000405 DAORVN010000000
VERSION     DAORVN010000405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2080
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56983_length_2080_cov_2.819753"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(155..628)
                     /locus_tag="PKV23_08265"
     CDS             complement(155..628)
                     /locus_tag="PKV23_08265"
                     /inference="COORDINATES: protein motif:HMM:NF016221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HQX85467.1"
                     /translation="MRLFKEIAFAATLAIASLYNIDTGIAHGVSIGNLEIEHPWSRET
                     KHGMHMAAGFMSITNNGAEDDRLIKATAEVSDTVQLHNMKMENDVMSMFEMKDGILIP
                     AGQTVELRPMSLHVMFMEMKSRPKQGEKFKGTLTFEKAGTVAIEFEVEASDAGMQ"
     gene            complement(870..1286)
                     /locus_tag="PKV23_08270"
     CDS             complement(870..1286)
                     /locus_tag="PKV23_08270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85468.1"
                     /translation="MTLPGLDSHNPFMCQGILKWLRKHGRLPSLFLLLAVVAPVLLSA
                     VPASAEQRLIRDLSYSICAPAEDGGSGERMPVFHEHCCILSGSAGHVFGMAAAAPMFI
                     LPPLNIRSVDVVLAATDIPSSPPDLRATAPRGPPTV"
     gene            complement(1309..>2080)
                     /locus_tag="PKV23_08275"
     CDS             complement(1309..>2080)
                     /locus_tag="PKV23_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004687280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HQX85469.1"
                     /translation="FGLAQLYQLRGRVGRSKHRAYSLFTTPPNRVLTEAADRRLKVLQ
                     SLDSLGAGFTLASHDLDIRGSGNLLGEEQSGNIKEVGYELYQTMLEEAVIKMRTGGEM
                     EEAEGTWSPQINVGTSVLIPERFVSDLQVRLGLYRRLADLHEQSDLEAFGAELHDRFG
                     RLPEEVQHLIEVVSIKLLCRAANVSNVDAGPKGVVLGFRKDTFPNPTALVQWIAGQGT
                     LGKLRPDMKLVILRTWKTPEERLKGTRQIMQNLVKLAA"
BASE COUNT          451 a          574 c          624 g          431 t
ORIGIN      
        1 ccgctgctgg acgccaggcg gaatggcccg ccaatatcag ccattgaaac cagcgtgtta
       61 cgcacctcat tgccctggaa atagagaacc ccgaagacgg cggccgtgat ggccgccatc
      121 ccaaggatgc ccaagcggag tgcctgaatg aaactcattg catgcctgcg tcagaggctt
      181 caacttcgaa ttcaatggca acagtacccg ccttctcaaa cgtcagcgtg cccttgaatt
      241 tttcaccctg cttgggacgg gacttcattt ccatgaacat gacatgcagt gacatgggcc
      301 tcagttcgac agtctggcct gccggaatga gaatgccgtc cttcatttca aacatggaca
      361 tgacatcatt ttccatcttc atattgtgaa gctggactgt atcgcttacc tcggctgtgg
      421 ccttgatcag cctgtcatcc tcagccccat tgttggtgat ggacataaag ccggccgcca
      481 tatgcatgcc gtgcttggtc tcccgcgacc atggatgctc aatctccaga ttgccaatgg
      541 aaacaccatg ggcaatgccg gtgtctatat tgtagagtga agcaatggcc agggttgctg
      601 caaatgctat ttccttgaag aggcgcatga ttttttcctt tgcgaattga gttacttgaa
      661 catgaacttg agcgcgggca ccgcgacggg cgccatgtct gtcatgggaa atgccagcgc
      721 caggcggcgc ggtagaaagc ggatgatgcg gcttttgcag ccggttccgc ccggaccggg
      781 actttcgaca tctgatatct ccaaatttgg gataaagccc ggcaggaatc gtgacctgtc
      841 aggacattga attcggaact ttttggaaat cagacggttg gggggccgcg cggggcggtt
      901 gcgcgcaaat cgggcggcga ggaaggaatg tcagttgcag ccaaaacaac atcaacgctt
      961 cttatgttga gcggcggcag aatgaacatg ggagccgccg cggccattcc aaatacatgg
     1021 ccggcggaac cggacaggat gcagcaatgt tcgtggaaaa cgggcatccg ttctccgctg
     1081 ccgccgtctt cggctggggc gcagatgcta taggaaaggt cccgtataag ccgctgttcg
     1141 gcgctggccg gcacggcaga taacagaacc ggagcgacaa cggccagcag caagaagaga
     1201 gatggaagcc tcccgtgctt acgcagccat ttcaatattc cctgacacat aaaaggatta
     1261 tggctgtcca gcccgggcag cgtcaccgtg acagaacccc gcacggaact atgcggccag
     1321 cttcaccaga ttctgcatga tctggcgggt gcccttcaga cgctcttccg gggttttcca
     1381 ggtgcggagg ataaccagct tcatgtcggg ccggagcttg ccgagagtcc cctgcccggc
     1441 gatccactgc accagggctg tgggattggg gaaggtgtcc ttgcggaagc cgagcaccac
     1501 gccttttggc ccggcatcaa cattcgagac gttggcggcg cggcagagga gcttgatgga
     1561 gacaacctcg atgagatgct ggacctcttc aggcaggcgg ccgaaacggt catgcagttc
     1621 ggcgccgaag gcttcgagat cggattgctc gtgaagatcg gcaagccggc gatagaggcc
     1681 gaggcggact tgcagatcgg agacaaatct ttccggaatc agcaccgagg ttcccacatt
     1741 gatctgcggc gaccaggtgc cttcagcctc ctccatttcc ccacccgtgc gcatcttgat
     1801 cacggcttct tccagcatgg tctggtagag ttcgtagccc acttccttga tgttgccgga
     1861 ctgctcttcg cccaggagat tgccggagcc gcggatatcg aggtcgtgac tggccagggt
     1921 aaagcctgca cccaacgagt cgagtgattg caggactttc agtcggcggt cggcggcttc
     1981 ggtgagcaca cggttcggtg gcgtggtaaa gagggaatag gcgcgatgct tggaacggcc
     2041 gacgcggccg cgcaactggt agagctgggc gaggccaaac
//
LOCUS       DAORVN010000406         2078 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_57105_length_2078_cov_2.688581, whole genome shotgun sequence.
ACCESSION   DAORVN010000406 DAORVN010000000
VERSION     DAORVN010000406.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57105_length_2078_cov_2.688581"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..556
                     /locus_tag="PKV23_08280"
     CDS             <1..556
                     /locus_tag="PKV23_08280"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AsmA-like C-terminal domain-containing protein"
                     /protein_id="HQX85470.1"
                     /translation="VAGGSLEFSAFLANGGTSTIQNGRLVMRDFDVRNEAALADIDSR
                     SKSKKSGPRREGLSFTKLTLPFTTDAKFIRIGDSLLKGNELGAAAEGLIRKADGAIDF
                     NGTIIPAYGINAAVGNIPLFGEIFTGGKGQGIFGLTFALGGTMANPKIQYNPLSAMAP
                     GILRKIFEYDGSGPPAKQKVKETN"
     gene            complement(557..1807)
                     /gene="tyrS"
                     /locus_tag="PKV23_08285"
     CDS             complement(557..1807)
                     /gene="tyrS"
                     /locus_tag="PKV23_08285"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015666797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HQX85471.1"
                     /translation="MSTYKSDFLNTLSTRGYIHQCSDAAGLDALAAKGDVTAYIGFDC
                     TAKSLHVGSLVQIMLLHWAQELGQKSIALMGGGTTRVGDPSGRDESRKLLTLEDIEDN
                     KRGIQQVFAKFLRFGSGPKDALMIDNAEWLTKLNYLDMLRDIGRHFSVNRMLAMDSVK
                     LRLEREHELSFIEFNYMVLQAYDFAELARRYGCNLQMGGSDQWGNIINGIDLGRRMGT
                     HQLFALTTPLITLASGAKMGKSASGAVWLNADMLSPYGYWQFWRNTEDADVGRFLKLF
                     TTLPLAEIAKLEALGGSEINIAKKTLADKATALLHGQQAAEAASETARRTFEDGESAE
                     GLPTIELDLENGAGVLQAAVQAGFASSNGEARRAIQGGALRINDMAVIDEKMQLTMSN
                     LNSEGVIKLSMGKKKHALIKPRQA"
     gene            1838..>2078
                     /locus_tag="PKV23_08290"
     CDS             1838..>2078
                     /locus_tag="PKV23_08290"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HQX85472.1"
                     /translation="MSKLTTALGLMSGTSLDGIDVALIRTDGENTVERGPSQTYPYSE
                     AQRHVLRAALKDAKGLARRDERPFGLARAEAYVTDW"
BASE COUNT          423 a          630 c          586 g          439 t
ORIGIN      
        1 agttgccggc ggcagtcttg agttttccgc cttcctggca aatgggggca catcaaccat
       61 tcagaacggc cgactggtga tgcgcgattt tgatgtccgc aacgaagccg ctcttgccga
      121 tatcgacagc cggagcaaat cgaaaaagtc cggcccgcgc cgcgaagggc tttccttcac
      181 caagcttacc ttgcctttca caaccgacgc gaaattcatc aggattggcg attcactgct
      241 caagggcaat gagctcggag ctgccgccga gggcctgatc cgcaaagctg atggcgccat
      301 agatttcaat ggaacaatca tccctgccta tggcatcaac gcggccgtcg gaaacatacc
      361 gctgtttggc gaaatcttca ccggcggcaa ggggcagggc atttttggcc tcacctttgc
      421 gcttggcggg accatggcga acccgaagat tcagtataat cccctgtcag ccatggcgcc
      481 gggaattctg cgcaagatat ttgagtatga tggctccggc ccaccggcta agcagaaggt
      541 caaggaaacc aactgatcag gcctgccttg gtttgatcaa tgcgtgcttc tttttcccca
      601 tcgacagttt tatgacgccc tcgctattca ggttcgacat tgtgagctgc attttctcat
      661 cgataaccgc catgtcattg atgcgaaggg cgcctccctg aatggcgcgc cgagcctcgc
      721 catttgacga tgcgaaaccg gcctgcactg ccgcttgaag aacgccggca ccattttcaa
      781 ggtcgagctc gatggtcggc aggccttccg cgctttcgcc gtcctcaaat gtcctgcgcg
      841 ccgtttctga cgcagcttct gccgcctgct gcccgtgcag cagggcggtg gctttgtcgg
      901 ccaacgtctt cttggcgatg ttgatttccg agccccccag ggcctccagt tttgcgatct
      961 cggccagggg aagcgtggtg aaaagcttaa ggaagcgccc cacatcggca tcttcggtat
     1021 tgcgccagaa ctgccagtag ccgtatggcg acagcatgtc cgcgttgagc cagacggcgc
     1081 cgctcgccga cttgcccatc ttggcacccg aggccagcgt gatcagcggc gtggtcaggg
     1141 caaaaagctg atgcgtgccc atgcgccggc caaggtcgat gccgttgatg atgttgcccc
     1201 actggtccga gccgcccatc tgcaggttgc agccatagcg ccgcgccagc tccgcaaagt
     1261 cataggcctg gagaaccatg tagttgaact cgatgaacga aagttcatgc tcccgttcca
     1321 accgcagctt gactgaatcc atcgccagca tgcggttgac tgaaaagtgc cggccgatat
     1381 cgcgcagcat gtcgagatag ttcagcttgg tcagccactc cgcattgtcg atcatcaacg
     1441 cgtctttcgg ccccgagcca aagcgcagga acttggcgaa aacctgctga atgccgcgct
     1501 tgttgtcttc gatgtcttca agtgtcagta acttgcggct ctcgtcgcgc cccgaaggat
     1561 cgccaacccg cgtcgtgccg ccgcccatca gggcgattga tttctgcccc agttcctggg
     1621 cccagtgcag cagcatgatt tgcaccagcg agccgacgtg gagggatttt gccgtgcagt
     1681 cgaaaccgat gtaggctgta acatcaccct tggccgcaag cgcgtcgagg cctgccgcat
     1741 cggaacattg gtgaatgtag ccccgggttg aaagggtatt aagaaagtcg gacttatatg
     1801 tgctcataat gggctgctca tagcatggga taaagcagtg tcaaaactaa ccaccgcact
     1861 aggcctgatg tccggcacct cgctggacgg cattgatgtc gccctcattc gcaccgacgg
     1921 cgaaaacacc gtcgagcgcg ggccatccca gacctatccc tattccgagg cccagcgcca
     1981 cgtattgcgg gctgccctga aggatgccaa aggccttgcg cgccgtgatg aaaggccctt
     2041 cggcctggcc agggcggaag cctacgtcac cgattggc
//
LOCUS       DAORVN010000407         2068 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_57577_length_2068_cov_3.204670, whole genome shotgun sequence.
ACCESSION   DAORVN010000407 DAORVN010000000
VERSION     DAORVN010000407.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2068
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57577_length_2068_cov_3.204670"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1105
                     /locus_tag="PKV23_08295"
     CDS             <1..1105
                     /locus_tag="PKV23_08295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85473.1"
                     /translation="LSPEIERALDMCYDAIAAPQLWMGALDNLAHSMGAHACQILPHV
                     ISQRSIGEVRSTGAWRIEELWLRNQDWATDVYVARGEPYVQQGYRAVIQSQLFSDEEI
                     RHSRFHQEITRPVGCLHWACGIFQVEDRVWCLPFYRGNEPFAADVLQPIAEVAGRMAH
                     IVGLAEKISRAGVENEVFTLDRAGCAAMLIDKSGRVTRVNSRAEDLFCKDFGVRNGRP
                     WAAANASLERLDRFMAEIELARSTAGPLPAPVIIARNNTPWLLIEAMPVTSASNDIFE
                     GCRALLVVSDLSRASITDATVLSLVFGLTSAEARLAAAICEGQDLNAASVSFGVSRET
                     LRSQLKTVFAKTGSRRQAELVARAAQIRNITQH"
     gene            1302..1748
                     /locus_tag="PKV23_08300"
     CDS             1302..1748
                     /locus_tag="PKV23_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007692433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HQX85474.1"
                     /translation="MRSSSLTPTVRVWGGRIMSKSRAVKFALAFGFVSVTFAGSALAE
                     GDIVAGEKLFLRKCFGCHVVPAGAVAKGPSLRGVVGRPVASVEGFAFSDGLKAFGATG
                     AVWDEATLDKFLADIIGFVRGMRMTMMPISRDTDRADLIAYLKSNK"
     gene            1858..>2068
                     /locus_tag="PKV23_08305"
     CDS             1858..>2068
                     /locus_tag="PKV23_08305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85475.1"
                     /translation="MIVAGEDNPSDMAADEIGHASHCPAHGAVVVRLGQEVLSVNLSP
                     QTERAMELCYDSISAPQLWSGALDNL"
BASE COUNT          411 a          559 c          631 g          467 t
ORIGIN      
        1 tttgtctcca gaaatcgaac gtgccctgga catgtgttac gatgccattg ctgcgccgca
       61 actttggatg ggggcattgg ataatttggc gcattcgatg ggcgcacatg cctgtcaaat
      121 cctgccgcat gtcatctcac aacgttccat tggcgaggta cgctccaccg gtgcctggag
      181 gatagaggag ctttggctca ggaaccagga ttgggcgacg gatgtttatg tggcccgggg
      241 agaaccttat gtccagcagg gctaccgtgc ggtcatccaa tcgcagctct tcagcgacga
      301 ggaaatcagg cattcgcgct ttcatcagga aatcaccagg ccagtcgggt gcctgcactg
      361 ggcgtgcgga atatttcagg ttgaagaccg ggtctggtgc ctccccttct atcgcggcaa
      421 tgaacctttc gccgccgatg tcttgcagcc cattgcggaa gttgccggac gcatggcgca
      481 catcgttggt ttggcggaaa aaatctcgcg ggctggcgtg gagaacgaag ttttcacgct
      541 cgacagagcc ggatgtgcgg caatgctcat tgacaagtct ggacgcgtca cgcgcgtgaa
      601 cagtcgcgcc gaggatctgt tctgcaagga tttcggagtt cgaaacggaa ggccttgggc
      661 ggccgcaaat gccagcctgg agcggcttga ccgctttatg gcggaaatag aactggcacg
      721 atccactgcg gggccgcttc cagcccctgt gatcattgcg cgcaacaaca cgccctggct
      781 gctcattgag gcaatgccgg tgacttccgc atcgaacgat atttttgaag gctgccgcgc
      841 ccttctcgtc gtgagcgacc tgagccgggc ttccatcact gacgccaccg tgctcagctt
      901 ggtgttcggg cttaccagcg ccgaagcgcg cctggccgcc gctatctgcg agggccaaga
      961 tctcaatgcg gcatccgttt ccttcggggt gagccgcgaa acactgcgca gccaactgaa
     1021 gactgtgttt gccaagaccg gttcgcggcg acaggcagaa cttgtggcgc gcgccgccca
     1081 aatcagaaac atcacccagc attgattgtc acacccgttt gggggatgcg ggatttccca
     1141 aaatggcgga cttttcccca agcctaagcg ggcatcgata gaggggaggt tgcatgtcta
     1201 cgttagcacg ccgccaattc attattaatg ggagcgcggc tctcgccgcc ccagccgttt
     1261 tcaagtcatg cttcgtccaa gcctggaact tgcgagcagc aatgagaagc agcagcctca
     1321 cccccacggt gcgggtttgg ggcgggcgta tcatgtcgaa gtcgcgtgca gtgaagtttg
     1381 ccttggcttt tggatttgtg tcagtcacat ttgccgggtc ggccctggct gaaggcgata
     1441 tcgtggcagg ggagaaactc tttctcagaa agtgctttgg ctgccatgtg gtacctgccg
     1501 gagccgttgc gaaggggcca agtctgcgag gtgtcgtcgg ccgccctgtg gccagtgtgg
     1561 aaggctttgc tttctccgat ggattgaagg cttttggggc cacgggagcc gtctgggacg
     1621 aggctacgct tgacaagttt cttgcagata tcattggctt tgtaaggggc atgaggatga
     1681 caatgatgcc tatcagccgg gacaccgacc gggcggacct gatcgcatat ctcaaatcca
     1741 ataagtaaca gtattggcct gttccgattt tcaagaaaaa gcccctggct gacgaattca
     1801 ttttgtcgtc actgtcacca gagatgtcac tggcttgcca tccacgagga tagggttgtg
     1861 atcgtcgccg gtgaggataa tccctctgac atggccgccg atgagatcgg gcacgcctca
     1921 cattgccccg cgcatggagc ggtggtagtg agacttgggc aggaggtact ttccgtgaat
     1981 ttgtctcccc agaccgagcg cgccatggag ttgtgctacg attcaatctc agcgccgcag
     2041 ctgtggtcag gtgcgctgga taatttag
//
LOCUS       DAORVN010000408         2062 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_57871_length_2062_cov_2.826109, whole genome shotgun sequence.
ACCESSION   DAORVN010000408 DAORVN010000000
VERSION     DAORVN010000408.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57871_length_2062_cov_2.826109"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..537
                     /locus_tag="PKV23_08310"
     CDS             <1..537
                     /locus_tag="PKV23_08310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HQX85476.1"
                     /translation="AKIYSALKTIRAWNHPLTVPVYLAFALASGGALLTAIATIFDRF
                     QIFQVILTGAALLVLIALKFFYWRGIDSAERTHTMAAATGLGAGVRQWEVPHTSENYI
                     MKEMGFAVARKHAAKLRTGVLLLMIMTLLALTACLVSPWFSLIGALAILLAAVLERWL
                     FFAEAQHVVSLYYGTEKA"
     gene            539..1357
                     /locus_tag="PKV23_08315"
     CDS             539..1357
                     /locus_tag="PKV23_08315"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQX85477.1"
                     /translation="MLADGVRWFDDWFAIEDIAPGVYAIGEPRFHQINWNYLIEGQDT
                     VLLFDTGPGVRDISQVVRALTDFPLITLPSHLHFDHTGNLQRFQNIALADLPVLRSCM
                     RDGLLHASDDLFRGFLEGMVWKPVKISQWLPIGSRIDLGGRQLEVLHTPGHSPDSISL
                     FDREANILFAADFVYPGPLYAQVPGANLADYLTTSVALLTQIDDQTKIFCGHGAPDEK
                     GKHRAPLMGSQDISDLQKSLASLKDSGKTPKETTVNARMTLLANKAAYASWQAG"
     gene            1495..>2062
                     /gene="minC"
                     /locus_tag="PKV23_08320"
     CDS             1495..>2062
                     /gene="minC"
                     /locus_tag="PKV23_08320"
                     /inference="COORDINATES: protein motif:HMM:TIGR01222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HQX85478.1"
                     /translation="MGRSYLAFVLAPELPFVDWLVELDRRVENSLSFFANRPLVLDLT
                     GVAPTKAETAEIIAFIEERHLRLIAVEGVPLDWLPERLAPLPGKVQTAGVFQPPESEK
                     PGRSAPPVTWENEPSIGSPCAALLVNEPVRSGQSIIYPHGDVIIVGSVASGAEIIAGG
                     SIHVYGSLRGRASAGTNGNTNARIFCSKF"
BASE COUNT          386 a          625 c          555 g          496 t
ORIGIN      
        1 gcaaaaatct actctgctct gaaaaccatc cgcgcctgga atcacccgct tactgtgccg
       61 gtctatctcg ctttcgcgct cgcctcgggc ggggctttgc tgacggcgat tgccaccatc
      121 ttcgatcgat tccagatctt ccaggtaatc ctgaccggtg ccgcactgct tgtgctgatt
      181 gccctgaagt ttttctattg gcgcggcatc gacagcgccg aacggaccca caccatggcc
      241 gcagccaccg ggctcggcgc cggtgtgcgc caatgggaag tgccgcacac ctccgaaaat
      301 tacatcatga aggaaatggg ttttgccgtg gcacgaaagc acgcggcgaa actgcgcact
      361 ggcgtattac tcctcatgat catgacgttg ttggcgctta ccgcttgcct tgtttcaccc
      421 tggttttcgc tgattggagc gcttgcaatt ctgttggccg ctgtgctgga acgctggctg
      481 ttctttgccg aagctcaaca tgtggtatcg ctctattatg gaacagaaaa agcctagcat
      541 gctggccgat ggggtgcgct ggttcgacga ctggtttgcg attgaagaca tcgcgccagg
      601 tgtttatgcc atcggcgagc cgcgcttcca tcagatcaac tggaattacc tgatcgaggg
      661 gcaggacacc gtgctgctct tcgataccgg tcccggtgtg cgcgacatct cgcaggtggt
      721 gcgagccctg acagattttc cgctcatcac cctgccatcg catctgcact ttgatcacac
      781 tggcaatctg cagcgctttc agaatattgc cttggctgac ttgcctgtct tgaggtcctg
      841 catgcgtgac ggcctgcttc atgccagcga tgatctcttc cgcggcttcc tcgaaggcat
      901 ggtttggaag cccgtgaaaa tttcacaatg gttgcccatc ggcagtcgaa ttgatcttgg
      961 cgggcgtcaa ttggaagtgc tccatacgcc cggccattca cccgattcga tttcgctttt
     1021 tgatcgggag gcaaacattc tatttgcggc cgattttgtt tatcccggcc ccctatatgc
     1081 gcaagtccct ggtgccaacc tcgccgacta cctcacgaca tccgtggctc tattgaccca
     1141 gatagatgat caaacaaaaa ttttctgcgg ccacggcgcg cctgatgaaa agggaaagca
     1201 tcgggccccg ctgatgggaa gccaggatat ttccgacctt caaaagtccc tcgccagcct
     1261 caaggattcg ggcaaaacgc cgaaagaaac aacggtaaat gccagaatga cgctccttgc
     1321 caacaaggcc gcctatgcct cctggcaagc gggctaagcc atcttttccc tgaacgcgac
     1381 actccgttaa cggattgtta accatgtctg gtcctaatcg ctgctgttgg ggtgcgttgc
     1441 gtgagggtgt tcaatttgtc agttgctgtt caatcacgga tttcatttcg tctaatgggc
     1501 cggtcctatc tggcctttgt cctggccccc gagctgccct ttgtggattg gctcgttgaa
     1561 ctggaccgca gggtggagaa ctccctgagc ttcttcgcca accgtccctt ggtgctcgat
     1621 ctcacaggtg tcgctcccac caaagccgag actgccgaga tcattgcctt catagaagag
     1681 cgccacttgc gtctcatcgc tgtggagggc gttccgttgg actggttgcc cgagcgcctg
     1741 gccccgctcc ccggcaaggt ccagacggca ggcgtgttcc agccgcctga atcagaaaag
     1801 ccgggccggt cagcaccgcc cgtgacctgg gagaatgagc cttccatcgg ttccccttgc
     1861 gcggctttgc tcgtcaacga gccggtccgc tccggccagt cgatcatcta tccccatggc
     1921 gatgtcatca tcgttggctc ggttgcttcc ggcgctgaaa tcatcgccgg cggctccatc
     1981 catgtctatg gcagcctgcg cggccgcgcc tctgccggca ccaacggcaa caccaatgcg
     2041 cgcattttct gcagcaagtt cg
//
LOCUS       DAORVN010000409         2060 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_57959_length_2060_cov_3.501746, whole genome shotgun sequence.
ACCESSION   DAORVN010000409 DAORVN010000000
VERSION     DAORVN010000409.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2060
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57959_length_2060_cov_3.501746"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..735
                     /locus_tag="PKV23_08325"
     CDS             <1..735
                     /locus_tag="PKV23_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008139023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQX85479.1"
                     /translation="FDAVLMTGGAEKPRDLPIPGRELAGIHPAMDFLPQQNRRVSKES
                     PLDNKPILAGGKHVIVIGGGDTGSDCIGTSLRQGALSVTQLEIMPRPPDKENKALVWP
                     DWPLKFRTSSSQQEGAERDFSVVTKSFSGENGEVKRLHCVRVDAKMQPIAGTEFELKA
                     DLVLLAMGFLGPVQEGMLASLGVKLDGRGNVEANVMNYKSSLPKVFSAGDMRRGQSLV
                     VWAIREGRQAARAVDEFLMGESTLPR"
     gene            complement(732..1787)
                     /locus_tag="PKV23_08330"
     CDS             complement(732..1787)
                     /locus_tag="PKV23_08330"
                     /inference="COORDINATES: protein motif:HMM:NF016218.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF459 domain-containing protein"
                     /protein_id="HQX85480.1"
                     /translation="MLLRLAIIILGLSLAGASAQAQSVETAPLMGSQSTEEEIVQPVD
                     DTYRILVIGDALAGGLGAGLSRMAEPEPRFEIVNRFQETSGIARPEVYDWAASLPKIM
                     EGKEFNAVVVLMGSNDRQAIREGDFRLAFNTPEWKAAYEARIDELLEVLKSAGVKVFW
                     VAIPPMGDAKYEADMKSIAALQKQEVLGKGQVYVDLRSAFLTPDGEYTDKGPDDTGEV
                     QRLRSRDGVAFLRLGNNRFGQLLLAEIKRIIGPGAIDATPELSPKGDLPSPAVAVVPL
                     FGQDSGDGTAFTFQPDEKLISELARVVAGDIIVPAISRSDIVPGSEAEKLFILGQAQP
                     APAGRFDDFSYVKPTPN"
     gene            complement(1796..>2060)
                     /locus_tag="PKV23_08335"
     CDS             complement(1796..>2060)
                     /locus_tag="PKV23_08335"
                     /inference="COORDINATES: protein motif:HMM:NF013625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HQX85481.1"
                     /translation="AVGHLGDRIRGGGPIVGTWPDLDYDLSFAQRVDLQKRLSRLGFE
                     TGGADGRFGARTYEAIIAYQKSVGLPLDGKPSAKLLERLRNAG"
BASE COUNT          398 a          620 c          654 g          388 t
ORIGIN      
        1 tttgatgccg tcctgatgac gggcggggcg gaaaagccgc gcgatctgcc gatcccaggg
       61 cgcgagcttg cgggcattca tcccgccatg gatttcctgc cgcagcagaa ccgccgcgtc
      121 tcaaaggaat caccgcttga caacaaaccc attctggccg gcggcaagca tgtgattgtc
      181 atcggcggcg gcgatacggg atccgactgc atcggcacgt cgctgcgcca gggggccctg
      241 tcggtgaccc agcttgaaat catgccgcgg ccgccggaca aggaaaacaa ggcgctggtc
      301 tggcccgact ggcccttgaa attccgcacc tcgtccagcc agcaggaagg ggccgagcgg
      361 gatttctcgg tcgtcaccaa atccttctca ggtgagaacg gcgaggtgaa gaggctgcat
      421 tgcgtcaggg ttgacgccaa gatgcagccc attgccggaa ccgagttcga gctcaaggct
      481 gatctcgtgc tgctcgccat gggtttctta ggcccggtgc aggagggcat gctggcctcg
      541 ctcggcgtca agctcgatgg ccggggcaat gtggaagcca atgtgatgaa ctacaaatca
      601 agcctgccca aggtgttttc cgccggtgac atgcggcgcg ggcaatcgct ggtggtgtgg
      661 gcgatccgcg agggccgcca ggcggcgcgc gccgtcgatg aatttctcat gggtgagagc
      721 acgctgccgc gctagttggg ggttggcttg acgtaactga aatcgtcaaa gcggccagcg
      781 ggtgcgggct gggcctggcc gagaatgaaa agtttctcgg cctcgctgcc aggcacgata
      841 tcggaacgcg aaatggccgg aacgatgata tctccggcaa caacccttgc cagttcggaa
      901 attagtttct catcgggctg gaaggtgaag gcggttccgt cgccgctatc ctggccgaag
      961 agcggaacca cggctacggc aggcgacggc aggtcacctt ttggcgacaa ttccggcgtg
     1021 gcgtcaatcg cgcctggacc gatgatccgc ttgatttcag ccaggagaag ctggccaaag
     1081 cgattgttgc ccaatctcaa gaatgcaacg ccgtccctgg agcgcagccg ttgcacctcg
     1141 cccgtgtcgt cggggccctt gtccgtgtat tcgccgtccg gcgtcaggaa ggcagaacgc
     1201 aaatccacat agacctggcc cttgcccagc acttcctgtt tttgcagggc ggctatgctc
     1261 ttcatgtcgg cttcatattt ggcgtcgccc atgggcggga tggcaaccca gaacaccttc
     1321 acgccggccg acttgagaac ctcgagcaat tcatcgatcc tggcctcgta ggccgctttc
     1381 cattccggcg tattgaaagc cagccggaag tcaccttcgc gaatggcctg gcggtcgttt
     1441 gaccccatca atacaacgac ggcgttgaat tccttgcctt ccatgatctt gggaaggctg
     1501 gcggcccagt catagacttc cgggcgggca atgcccgagg tttcctgaaa acggttgacg
     1561 atctcgaagc ggggttcagg ttcggccatg cggctgaggc cggcgccaag gccgcccgcc
     1621 agagcatcgc cgatgaccag tatgcggtag gtgtcatcaa ccggctggac aatctcctct
     1681 tcggtcgatt gcgatcccat gaggggtgcc gtttccaccg actgggcctg cgcagaagcg
     1741 ccggcaagac tgaggccgag gataatgatg gcaagacgga gcaacatggc ccatcctaac
     1801 ccgcgttccg caggcgctca agcagcttgg cgctgggctt gccatcgagc ggcaggccga
     1861 cactcttctg gtaggcgatg atggcctcat aggttctggc cccgaagcgg ccatcggcgc
     1921 cgccggtttc aaagcccaga cgcgacaggc gcttctgcag gtctacccgc tgggcaaagc
     1981 tgagatcata atcgagatcg ggccaggtgc cgacaatggg gccgccgccg cgtatccgat
     2041 cgcccaaatg gcccacggca
//
LOCUS       DAORVN010000410         2059 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_58026_length_2059_cov_3.165669, whole genome shotgun sequence.
ACCESSION   DAORVN010000410 DAORVN010000000
VERSION     DAORVN010000410.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58026_length_2059_cov_3.165669"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            10..403
                     /gene="ssrA"
                     /locus_tag="PKV23_08340"
     tmRNA           10..403
                     /gene="ssrA"
                     /locus_tag="PKV23_08340"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01849"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="alphaproteobacterial-type; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01849"
     gene            499..1008
                     /locus_tag="PKV23_08345"
     CDS             499..1008
                     /locus_tag="PKV23_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011159039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor
                     SspB"
                     /protein_id="HQX85482.1"
                     /translation="MPEDLMRYDQLAQNALRGVVREALRRVEKSGLPGEHHFYIAFNT
                     KFPGVDIGERMAQRYPREMTIVLQHQYENLVVKEDRFEVELSFDNILEKLVIPFNAVK
                     GFLDPSVQFGLQFEVVSVEAPVDSEGKLEKSASGKVTRELEGAETSASDEPPKDGPKV
                     VSLDSFRKK"
     gene            1035..1961
                     /gene="mepA"
                     /locus_tag="PKV23_08350"
     CDS             1035..1961
                     /gene="mepA"
                     /locus_tag="PKV23_08350"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HQX85483.1"
                     /translation="MSFAAFLAAILFISAPLAQAQTAEDQAALAALTAADLKHTPAKK
                     LFGAKKLPVNLQARAIGSYAKGCLSGGKALSVTGPAWQAMRTSRNRNWAHPALVKLVE
                     KLAKESKQSDGWNGLLVGDMSQPRGGPMLTGHASHQIGLDADVWFTPMPDRVLTSAER
                     ETYVPVEMVKDRSTLNRDNWSESRARLIRRAASYPEVARIFVHPPIKAELCKWAKGDR
                     SWLAKVRPYYNHTYHFHVRMKCPPGFAGCKNQAPAAPKDGTGCGEEMAYWMGDIPWKP
                     KKRDPNAKPVKPAPALTLAGLPAECRAVVTAP"
BASE COUNT          451 a          632 c          589 g          387 t
ORIGIN      
        1 cccggaagcc ctatatctgg ggggccaccc agcaatgggc ggctatggtg ataaaatgcc
       61 atcgtaataa acctttcgga cccgggggca gtacccggcg cctccaccac agccgtttga
      121 cttcaaacgg cagacgccgg acagcagatt tccttaaatc tgcgataggg caacttagct
      181 gcaagcggct ttgatggggg cgaactagga tcgacgaggg tgtaaaggat ggagctttgc
      241 ccgtgatggt tccgacgtta tcgggctaaa caagtagttg caaatgacaa ctatgctccg
      301 gtacgtctcg ctgcttaatg cagtgcgaat gccgaaatca aagtcctgag gcttagccgt
      361 ttcaggcggg gtccgccggc acctggcaac agaagccggc actttcaatt gtgtgggaca
      421 aatgagttca tggctgcgga aagcttgaac ctgtagtatc tgaaggttga gtcggccttt
      481 ccaaaggatt tcgtgcctat gcctgaagac ctgatgcggt atgaccagct ggcgcaaaat
      541 gcgctccgcg gtgtggtgcg cgaggccctg cgacgcgtcg agaagtcagg ccttccgggc
      601 gagcatcact tctatatcgc cttcaacacg aaatttcccg gcgtcgatat cggcgaacgc
      661 atggcccagc gctacccccg tgaaatgacc atcgtgctcc agcaccagta tgaaaacctg
      721 gtggtcaagg aagaccgttt cgaggtggaa ttgtcattcg acaacatcct ggaaaaactg
      781 gtaatcccct tcaatgcagt gaagggcttt ctcgacccct cggtgcagtt tggcctgcaa
      841 ttcgaagttg tcagcgtcga ggccccggtt gactctgagg gaaagctgga gaaatctgcc
      901 tccggcaagg taacgcgtga actggaagga gctgaaacct ctgcttccga tgaaccaccc
      961 aaggacgggc ccaaggttgt ttccctggac tcgttccgca agaaatgagg accggacggc
     1021 ccatgcagat ttcaatgtcc tttgcagctt tcctcgctgc aatcctgttc atctcggcac
     1081 cgcttgccca ggcccagaca gccgaggacc aggcggcgct ggccgcgctg accgccgctg
     1141 acctcaagca cacgcccgca aagaagttgt ttggcgccaa gaagctgcct gtgaatttgc
     1201 aggcccgcgc cattggcagc tatgccaagg gctgcctctc tggtggcaag gcgctgtccg
     1261 tgaccggccc tgcctggcag gccatgcgca cctcacgcaa ccgcaactgg gcccacccgg
     1321 cacttgtcaa gctggtggaa aaactggcta aggaatccaa gcagagcgac ggctggaacg
     1381 gcttgctggt gggcgacatg tcccagcccc gtggcggccc aatgctcacc ggccatgcca
     1441 gccatcagat tggcctcgat gccgacgtct ggttcacgcc gatgcctgac cgggtgctaa
     1501 ccagcgccga acgtgaaact tacgttcccg tcgaaatggt gaaggaccgc tcgaccctca
     1561 accgcgacaa ctggagcgag tcccgcgcca ggctgatccg gcgcgccgcc tcatacccgg
     1621 aagtggcccg catcttcgta cacccgccga tcaaggccga actgtgcaaa tgggccaagg
     1681 gcgaccgctc atggctggcc aaggtacgcc cctactacaa ccacacctat cacttccacg
     1741 tccggatgaa atgccctccg ggttttgccg gctgcaaaaa ccaggccccg gcggccccca
     1801 aggacggcac cggctgtggc gaggaaatgg cctattggat gggcgacatt ccctggaaac
     1861 ccaagaaacg cgaccccaac gccaaacccg tgaaaccagc cccggccttg actctggcgg
     1921 gccttcctgc tgaatgccgg gcggttgtga cggcccctta gaccgtttcc ggaaatcatt
     1981 gagaagcgga gccctgatgg gcgatccatg acccaaagaa gctctcccgc aagcgatcca
     2041 gccctcacct ctggtacct
//
LOCUS       DAORVN010000411         2057 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_58125_length_2057_cov_3.440060, whole genome shotgun sequence.
ACCESSION   DAORVN010000411 DAORVN010000000
VERSION     DAORVN010000411.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2057)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2057)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2057
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58125_length_2057_cov_3.440060"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            152..382
                     /locus_tag="PKV23_08355"
     CDS             152..382
                     /locus_tag="PKV23_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011911471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HQX85484.1"
                     /translation="MKDIKVLGTGCANCRNTIKLIEDVAAEKGVEISLSKVEQLPEIM
                     KYGVMSTPGVVVDGKVVHAGGIPDRSKIAGWL"
     gene            complement(745..1359)
                     /locus_tag="PKV23_08360"
     CDS             complement(745..1359)
                     /locus_tag="PKV23_08360"
                     /inference="COORDINATES: protein motif:HMM:NF013460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HQX85485.1"
                     /translation="MSTRIFILPLWIRIWHWTNALLIITLTISGASLHFADPALVLVP
                     FALAARIHDMAGLALVAAYGFFAIANVITGNWWQYVPKPEGFIKKCVRQARFYAWGIF
                     HGEAPPYPPTPKNNFNAMQQIVYWFMMYLVMPILLITGVMFMWPDLAPKSVLGYDGLM
                     PVAVMHYLAALVIVSFMFAHIYLGTTGHKISTHFKMMITGWHEH"
     gene            complement(1356..>2057)
                     /locus_tag="PKV23_08365"
     CDS             complement(1356..>2057)
                     /locus_tag="PKV23_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008550640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming cytochrome c nitrite reductase
                     subunit c552"
                     /protein_id="HQX85486.1"
                     /translation="LPEPMYQGPGARIMSLKDGSKKMSKSDPSDASRINLLDTADDIA
                     KKIKRATSDSEPLPSEVKGLEGRNEAANLVGIYSVLAGKAEADVLAEFGGKVLPDKKR
                     PDIDKIHAWLPNAQRHWQSARCIDCHTPQVKANSALSVSHEILNKDKAQKNCSTCHAQ
                     DSALRTGLYRHVKETEAKEMGFANAAFLRNSYVVGATRNFYLDLLGLIMVGGTIGGVS
                     LHGLLRILAARRRKS"
BASE COUNT          468 a          536 c          612 g          441 t
ORIGIN      
        1 tcttcatggc cacgggtttc tgcgagagca aggaactcgt gattctcggc ctctccctgg
       61 ccaactggtc atttcttgcc tatgccggat tcactgccat ggcttcgctg attttgtttt
      121 atccctcaat ttccagaaag gaaaacgcat catgaaagac atcaaggttc tcggtaccgg
      181 ctgcgccaac tgccgcaaca ccatcaaact gatcgaagac gttgcggccg aaaaaggtgt
      241 cgaaatttcg ctttccaagg tcgagcaact tccggaaatc atgaagtacg gggtgatgtc
      301 cacgccaggt gtcgtggtcg atggcaaggt ggtgcacgcg gggggtattc ccgaccgcag
      361 caagattgcg ggctggctgt aatccgccgc cccaatcccg accgaggagg aaggagacct
      421 ccttggcgta ttgctgatgt actcccaacg ggcccatgtg ccgacgggct cctgaacggc
      481 gaaaagctga cagaaggcat cgaggtgcta ttgaaccaga ggcaaggacg tttgcttact
      541 tgatcgtccg atggaaaaac cccttcacgg catcacgaca atcagggcac acgctcagat
      601 gctcggcggg cagcccggcc tgctccaggg ctgttaatac tgccgcaaca ggtaaagatg
      661 atgtttggcg ttcttggaat ggatcgcaaa aaagcggctc tgctttcgca ttgccgctga
      721 gttacgagag atgaaatttg tgcgttagtg ttcgtgccaa ccagtaatca tcatcttgaa
      781 atgggtgctg atcttatgac cggttgttcc caggtaaatg tgggcaaaca taaagctgac
      841 gatgaccaag gccgccagat agtgcataac ggcaaccggc atcaagccgt cgtatccaag
      901 gacactcttc ggggccaagt ccggccacat gaacatgaca ccggtgatga gcaggatggg
      961 catgacaagg tacatcatga accagtagac aatctgctgc atggcgttga agttgttctt
     1021 cggagtcggt gggtagggcg gggcctctcc atggaaaatg ccccaggcgt agaaccgagc
     1081 ctgacgtacg catttcttta tgaagccttc cggtttcggt acgtattgcc accagttgcc
     1141 ggtaatcaca ttggcgatgg cgaagaagcc gtaggccgcg accagtgcca atccggccat
     1201 gtcatgaatt cgtgcagcca aggcaaatgg caccagaacc agggcagggt cagcaaagtg
     1261 caggctggcg ccgctgatcg taagggtgat gatcaacaaa gcgttggtcc agtgccaaat
     1321 gcgaatccag agcggcagga tgaagatgcg ggtactcatg atttcctccg gcgggcagca
     1381 aggatgcgga gcaggccgtg gaggctgaca ccgccaatgg tgccaccgac catgatcagg
     1441 ccgagcaggt cgagatagaa gttgcgggtc gctccgacaa cataggaatt gcgcaggaat
     1501 gccgcgttgg caaagcccat ttccttggcc tccgtttctt tgacgtggcg atataagccg
     1561 gtacgcaggg cactgtcctg ggcgtggcaa gtcgagcaat tcttttgtgc cttgtccttg
     1621 ttgaggattt catgggagac ggagagggcc gaattggctt tcacctgcgg cgtatggcag
     1681 tcaatacagc gggccgattg ccaatggcgt tgggcgttgg gcagccacgc gtgaatcttg
     1741 tcgatatcgg gccgcttctt atccggaaga accttgccgc caaattcggc aaggacatcc
     1801 gcttcggcct tgccagcgag aacagaatag atgccaacaa ggttcgctgc ttcgttgcga
     1861 ccctcaagac cctttacctc ggacggaagg ggctcggaat cagacgtggc gcgcttgatc
     1921 ttcttggcga tgtcatctgc ggtatcgagc aggttgatgc gcgaggcatc ggaaggatcc
     1981 gacttcgaca tcttctttga gccgtccttg agcgacatga tgcgcgcgcc gggaccctga
     2041 tacatcggct cgggcag
//
LOCUS       DAORVN010000412         2050 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_58480_length_2050_cov_2.858145, whole genome shotgun sequence.
ACCESSION   DAORVN010000412 DAORVN010000000
VERSION     DAORVN010000412.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58480_length_2050_cov_2.858145"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1810)
                     /locus_tag="PKV23_08370"
     CDS             complement(<1..1810)
                     /locus_tag="PKV23_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013533669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HQX85487.1"
                     /translation="MTEPVINTSPRTWDEVKTTTCYMCACRCGIKVYLKDGTVKYIEG
                     NRDHPVNKGVICGKGASGIKQHYSPARLSKPLMRVGERGSGEFREVEWEEALRTATLW
                     LSEIRNKDPRKLAFFTGRDQSQGLTGWWATQFGTPNYAAHGGFCSVNMAAAGLYTFGG
                     SFWEFGEPDWKHTKYFLLFGVAEDHASNPIKTGLGKLKARGAKFVSINPVKTGYSAIA
                     DEWVGLKPGTDGLFVGALIHELLAAQKIDGEYLQRYTNAPWLVIENPGGDDDGLFLRE
                     TSGAAQVWDSHTNSPMSANSSDASPKLMGRFALPDGRKVVTSFTLIAERFLSNDYAPD
                     TVAERCAISASTIRRIAAEIAEVAFEQQIELDVPWTDYTGRRHEKMIGRPVSMHAMRG
                     ISAHANGFQTCRILHILQIILGTIDVPGGFRYKPPFPKPIPPPIRPAGKLGQVRPNTP
                     MPGPPLGFVVSPNDLLVDEDGKPLRIDKAYSWDAPLAAHGLMHMVINNAAKGDPYTID
                     LLFMYMANMSWNSAMNVPDTLKALTAKRDDGEYVIPRIIYSDAYDSEMVHYADLILPD
                     TTYLERWDCISMLDRPISEADGPADSIRQPVVEPDRD"
BASE COUNT          413 a          561 c          648 g          428 t
ORIGIN      
        1 catcacggtc cggttcaacc acgggctggc ggatggaatc ggcggggcca tcagcttcgg
       61 aaatcggccg gtcgagcatc gaaatgcaat cccagcgctc gagataggtt gtatcgggca
      121 ggatgagatc ggcgtaatgc accatctcgg aatcataggc atcggaatag atgatgcgcg
      181 ggatgacata ttcgccatcg tcgcgttttg ccgtgagcgc cttcagcgtg tccggcacat
      241 tcatggccga attccagctc atgttggcca tgtacatgaa gagaagatcg atcgtgtagg
      301 gatcgccctt ggctgcgttg ttgatcacca tatgcatgag cccgtgggca gcgagcggcg
      361 catcccagga ataagccttg tcgatgcgca ggggtttacc gtcttcatcc accagcaaat
      421 cattggggct gacgacgaag cccagaggcg ggccgggcat gggcgtgttg ggccggacct
      481 gccccagctt gcccgcaggc ctgatcggcg ggggtatggg tttgggaaac ggcggcttgt
      541 agcggaagcc gccgggcaca tcgatggtgc ccaagatgat ttgaaggatg tgcaggatgc
      601 ggcaggtctg gaagccattg gcatgggcgg agatgccgcg catggcatgc atggaaacgg
      661 ggcggccgat cattttttca tgacgcctgc cggtataatc cgtccacggc acatcaagct
      721 cgatctgctg ctcgaaggcc acctcggcga tttcggcagc aatgcgccgg atggtgctgg
      781 cagaaatggc gcagcgttcc gccacggtat ccggggcgta atcattggag aggaatcgtt
      841 cggcaatcag ggtaaagctg gtcaccacct tgcggccatc gggcaatgca aagcgtccca
      901 tgagcttggg tgacgcatcg ctcgagttgg cggacatggg cgaattggtg tgcgaatccc
      961 agacctgggc cgcgccggat gtttctcgca ggaaaaggcc atcatcgtcg ccaccgggat
     1021 tttcgatcac cagccacggc gcattggtgt agcgctggag gtattcacca tcgattttct
     1081 gggcagccag caattcgtgg atcagggctc cgacaaaaag gccatcggtg ccaggcttga
     1141 gtcccaccca ctcatcggcg attgccgaat accccgtttt caccggattg atggagacga
     1201 acttcgcgcc cctggccttc aatttgccaa ggccggtctt gatcgggttc gaggcatggt
     1261 cttcggccac gccaaagagc aggaagtatt tggtgtgctt ccagtcaggc tcgccgaatt
     1321 cccagaatga cccgccgaaa gtgtagaggc cggcggcggc catgtttacc gagcagaagc
     1381 caccatgggc cgcatagttg ggggtgccga attgcgtggc ccaccagccg gtgagaccct
     1441 gggactggtc gcggccggta aagaaggcaa gtttgcgcgg atccttgttg cggatttccg
     1501 agagccacag ggtggcggtg cggagggcct cttcccattc gacttcgcgg aattcgccgg
     1561 agccacgttc gcccacccgc atcaggggtt tggagaggcg cgccggggaa taatgctgtt
     1621 tgatgccgga ggcgcccttg ccgcaaatca cccccttgtt caccggatga tcgcggtttc
     1681 cctcgatata tttgacagtt ccgtccttga gatagacctt aatcccgcag cggcaggcac
     1741 acatgtagca ggtggtggtt ttcacctcat cccaggtcct tggcgatgta ttgattacgg
     1801 gttcggtcat ggggttccag gatgcgacga ttccggacta cattagggcg cgggttggta
     1861 ctatgatagg tccagacggt tatttcctgg ggaccaaatc agttgcgcga ctggctcgtt
     1921 catatcaagc gttcggaaaa ctcaagcctg cagatgcaaa tccgcgaggc cctggtttcg
     1981 gcgattcttg accagcagct ggcgcgcgag gaacccattc cctccacccg caaaatggca
     2041 aagtcactca
//
LOCUS       DAORVN010000413         2049 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_58509_length_2049_cov_3.715145, whole genome shotgun sequence.
ACCESSION   DAORVN010000413 DAORVN010000000
VERSION     DAORVN010000413.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58509_length_2049_cov_3.715145"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..769
                     /locus_tag="PKV23_08375"
     CDS             <1..769
                     /locus_tag="PKV23_08375"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HQX85488.1"
                     /translation="ASQILDKRRQHDELASAAIIQQSFLPKTAKLDAIENILELHAEM
                     RPAREVGGDFYDFFMLDPDHLAIAIGDICGKGIAASLFMAVVVTVLRTAAHEEKDVAA
                     TIARANNVLSRDNASSMFATAFYAVLNLRTGVMEYCNCGHNAPFLILPSGETRSFPAT
                     GLPLALYADRTPAVARFTLSHSDIFVLFTDGVTEAMNASQEELGEAAFVEALAGSRLL
                     PVAEMVSRIFSTVDSFAQGAEQTDDITCLAIRRRPAI"
     gene            841..1671
                     /locus_tag="PKV23_08380"
     CDS             841..1671
                     /locus_tag="PKV23_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002712393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HQX85489.1"
                     /translation="MRCLVVADLHYSLPQFDWVLNVAGDFDLVIIAGDHLDLSSLVEG
                     RAQTVVIRKYIELMREKTKVVVCSGNHDLDSRDLTGEKVAKWIVDLAQHDVPSDGASL
                     VFEDTLFSICPWWDGPLTRDKIGEQLAADALKRTGRWFWVHHAPSDKSPTSWSGSRYL
                     GDNSLLEWVIQYKPDMVFSGHVHQSPFVKDGSWVDRIGDTWVFNVGFQFGAPPAHIIF
                     DTEKEEAVWLSAAGFQSIQFSQPLERPLRRLATPPAWVTLQDRAGGPSPAGIRPPAGE
                     "
     gene            complement(1607..>2049)
                     /locus_tag="PKV23_08385"
     CDS             complement(1607..>2049)
                     /locus_tag="PKV23_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007616054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HQX85490.1"
                     /translation="VPAGTVLLSEGNTSGFVYVLAEGQIEVLRGTTRVAVVSEPGSVF
                     GEMSVLLNTPHTATVRSLSPATVYAYADAAGFMRSDPQIAFVIARLLAQRLNSATTYL
                     VDLKRQYAGQGNHLGMVSDVLASLIHQQEAEFQPGSDRLPDPAM"
BASE COUNT          408 a          624 c          592 g          425 t
ORIGIN      
        1 cgccagccag attctcgaca agcggcgcca gcacgatgaa ctggcaagtg ccgccatcat
       61 ccagcaatca ttcctgccga aaaccgcgaa actggatgca atcgaaaaca tcctcgagct
      121 tcatgcggaa atgcggccgg cacgcgaagt gggcggcgac ttctacgact tcttcatgct
      181 ggatcccgat catctcgcca ttgccatcgg cgatatctgc ggcaagggaa tagccgcaag
      241 tctgttcatg gctgtggtgg tcactgtcct gcgcaccgcc gcccacgagg aaaaagacgt
      301 ggcagccacg atcgcgcggg ccaataatgt tctcagccgg gacaatgcat catcgatgtt
      361 tgccacggcc ttctatgcgg ttctgaatct gcgcaccgga gttatggaat actgcaactg
      421 cggccacaac gctcccttcc tgattcttcc ttcaggcgaa acccgcagct tcccggccac
      481 cggattgccc ttggcgcttt atgcggaccg caccccggcg gtggccaggt tcacgctcag
      541 ccattccgat attttcgtgc tgttcaccga tggcgtgacc gaagccatga acgcctcgca
      601 ggaggagctt ggcgaagcgg ccttcgtcga agcgcttgcc ggttcgcgcc tcttgcctgt
      661 ggccgaaatg gtgtcccgga ttttctcaac cgtcgattcc ttcgcgcaag gagcagaaca
      721 gaccgacgac attacctgcc tcgcaatccg ccgccgccca gccatttgac ggtgctcgga
      781 aaagtgtgat tatgtctggc gggtgccacg cagatcgcga cgtcaactgg ggaatttgac
      841 atgagatgcc ttgtcgttgc cgacctgcac tattccctgc cgcagtttga ttgggtgttg
      901 aatgtggcag gcgattttga tctggtgatc atcgccgggg accatcttga tttgagctcg
      961 cttgtcgagg gccgggccca gactgttgtc atccgcaaat acattgagtt gatgcgtgaa
     1021 aagaccaagg ttgtcgtctg ctccggaaat cacgacctcg attcgcgcga cctgactggg
     1081 gaaaaagtgg caaaatggat cgtggacctc gcccagcatg acgttccttc cgatggcgcc
     1141 tcgcttgtct ttgaagacac gctgttcagc atctgtccat ggtgggacgg cccgctcacc
     1201 cgcgacaaga ttggggaaca actggcggcc gacgccttga agcgcaccgg ccgctggttc
     1261 tgggtgcatc acgcgccgtc tgacaaatct cccacaagct ggagcggaag cagatacttg
     1321 ggtgacaatt ccctcttgga gtgggtcatc cagtacaagc ccgacatggt cttctccggc
     1381 cacgttcacc agtctccctt cgtcaaggac gggtcatggg tggaccggat cggcgatacc
     1441 tgggtgttca atgtcggatt ccagtttggc gcaccccccg cccacatcat tttcgacacg
     1501 gagaaagagg aagccgtttg gctttccgcc gcggggtttc aatccataca gttcagccag
     1561 ccgcttgagc gccccttgcg gcgccttgca acacctcccg cctgggtcac attgcaggat
     1621 cgggcaggcg gtccgagccc ggctggaatt cggcctcctg ctggtgaatg aggctggcaa
     1681 gcacgtcgct caccatgccc agatggttgc cctgccccgc atactggcgc ttcaggtcaa
     1741 ccagataagt tgtggctgaa ttcagccgct gggcgagcag gcgggcgatc acgaaggcga
     1801 tctgcggatc agaccgcatg aacccggccg catcggcata agcatagacc gtggctggcg
     1861 aaagcgagcg gaccgttgct gtatggggcg tattgagtag caccgacatt tcgccgaaga
     1921 ccgagcctgg ctcgctgaca accgcaaccc gcgtcgtgcc gcgcagcacc tcgatttgtc
     1981 cttcggcgag aacatagaca aagcccgacg tgttaccttc cgaaaggagc accgtccccg
     2041 cggggactt
//
LOCUS       DAORVN010000414         2049 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_58548_length_2049_cov_2.000000, whole genome shotgun sequence.
ACCESSION   DAORVN010000414 DAORVN010000000
VERSION     DAORVN010000414.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58548_length_2049_cov_2.000000"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2049
                     /locus_tag="PKV23_08390"
     CDS             <1..>2049
                     /locus_tag="PKV23_08390"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003682 - chromatin binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007076 - mitotic chromosome condensation
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chromosome segregation SMC family protein"
                     /protein_id="HQX85491.1"
                     /translation="DEQRQRIARLERDASETSGKRQALLEQMGGTGDGPTLAAAVDAA
                     LAVLASNETVANHAEVELRVARQVEADTRQAHDEARRKAEGLQTEVRTLTNLLKAADN
                     NLWPALVDQLKVERGYEAALGAALGDDIDASSDEGAPTHWRGLPPLDDGISLPGEALP
                     LSNFVQAPQALARRLSQIGVVSRSLGQALQAQLRAGQRLVSREGDVWRWDGFTSAADA
                     PSAAAKRLAERNRLSLLEIEMRDAAAVAAEAAQKFEAARQSVEAGVRAEREKRDAMRA
                     ATSSVDVSRQALARHERQIAERLAQTSALDEGIRRITQSLEEARSSFESAQAEFQAIP
                     VLEGLAEEVAALRDNVNRERAAYAEARARHDGLEREAKARADRINVIEAEARQWGERA
                     KRATEQIAHLTTRAQETREAIAELSGMPQILADKRHKLMNLLQEAETERKAAADALAE
                     GENAVRMADQVLREAQELASSSREDHARLEARLEASRQRHEDSERRIAETLQCAPSEI
                     VANAGLDLEKIEDTEHIERRLLSLREDRERLGGVNLRAEEEAETVATQLATLIRERDD
                     LVQAINKLRGGISSLNREGRQRLLDAFGTVNEKFGELFTTLFGGGKAELQLIESDDPL
                     EAGLEILANPPGKKPTVLSLLSGGEQTLTALALIFAVFLTNPSPICVLDEVDAPLDDH
                     "
BASE COUNT          400 a          634 c          711 g          304 t
ORIGIN      
        1 ttgacgagca gcgccagcgc attgcccggc ttgaaaggga tgcaagcgaa accagtggca
       61 agcgccaggc cctgctggag cagatgggcg gcaccggtga cgggccgact ctggccgctg
      121 cggtggacgc ggcccttgcg gttctggcct ccaatgaaac ggtggcaaac catgcggaag
      181 tggagctgcg ggtggcccgc caggtcgagg ccgacacaag gcaggcccat gacgaagcgc
      241 ggcgcaaggc cgagggcctg cagacggaag tccgtacact gacgaatttg ctgaaggccg
      301 ccgataacaa tttgtggccg gcgctggtgg accaactcaa ggtggagcgg ggctatgagg
      361 cggcgctcgg cgctgccctt ggcgatgaca tcgatgcctc atcggatgag ggggcgccca
      421 cccattggcg cgggcttccg ccccttgacg acggcatttc cctgcccggc gaagccctgc
      481 cgctttccaa ttttgtgcag gcgccacaag cacttgcgcg gaggctttca caaatcggtg
      541 tagtttcccg gtccttgggc caggcgctcc aggcgcagct gcgcgccggc cagcggctgg
      601 tgtcacgcga aggcgatgtc tggcgctggg acggctttac ctcggctgcc gatgcgccga
      661 gtgcggcggc aaagcgcctg gccgagcgca accggctttc gcttcttgaa atcgagatgc
      721 gcgatgcggc ggcggtcgcg gcggaagccg cccagaaatt cgaggcggcg cggcaatcgg
      781 ttgaagcggg cgtaagagcc gagcgtgaaa agcgtgacgc catgcgcgcc gctacttcat
      841 cggtagatgt atcccggcag gcgctggccc ggcatgagcg gcaaattgcc gagcgcctgg
      901 cgcaaaccag cgcgctggac gaaggcatcc ggcgcatcac gcaatcgctt gaggaagcgc
      961 gcagcagttt tgaatcggcg caagccgagt tccaggccat tccggtgctt gagggtctgg
     1021 cggaagaagt cgccgcactc cgcgacaatg tcaaccgcga gcgggcggcc tatgcggaag
     1081 cgcgcgccag gcatgacggg ctggagcgcg aggcgaaggc gcgggcggac cgcatcaacg
     1141 tgattgaggc ggaagccagg cagtggggcg agcgggcaaa acgcgccacc gaacagatcg
     1201 cgcatttgac aacgcgggcc caggaaaccc gcgaggcgat tgccgagctt tccgggatgc
     1261 cgcagattct ggcggacaag cgccacaagc tgatgaacct gctgcaggag gctgagaccg
     1321 agcgcaaggc tgcggctgat gccctggccg aaggcgagaa tgcggttcgc atggcagacc
     1381 aggttttgcg cgaggcccag gaactggctt cctcctcgcg tgaagaccat gcacgccttg
     1441 aagcccggct tgaggcttcc aggcaacgcc atgaggacag cgagcgccgc attgccgaaa
     1501 ccctgcaatg cgcgccgtcc gaaatcgttg ccaatgcggg cctcgacctc gagaagatcg
     1561 aggacacgga acatatcgaa cggcggctgc tcagccttcg cgaagaccgc gagcgcctcg
     1621 gcggggtcaa cctgcgggcc gaagaagaag ccgagaccgt tgcgacccag cttgcaacgc
     1681 tgatccgcga acgggacgac ctggtgcagg ccatcaacaa gctgcgcggc ggcatatcca
     1741 gcctgaaccg cgagggccgc cagcgcctgc ttgacgcctt tggcacggtg aacgaaaaat
     1801 tcggcgagct gttcaccacc ctgttcggcg gcggcaaggc ggaactgcag ctgatcgagt
     1861 ccgatgatcc gctggaagcc gggcttgaaa tcctggccaa tccgccgggc aagaagccaa
     1921 ccgtgctctc gctgctgtcg ggcggtgagc agacgctgac ggcgctggcg ctgatattcg
     1981 ccgtgttcct cacaaacccc tcgccgatct gcgtgctgga cgaagtcgac gcgccgctcg
     2041 acgaccaca
//
LOCUS       DAORVN010000415         2047 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_58621_length_2047_cov_4.024598, whole genome shotgun sequence.
ACCESSION   DAORVN010000415 DAORVN010000000
VERSION     DAORVN010000415.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2047
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58621_length_2047_cov_4.024598"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..428
                     /locus_tag="PKV23_08395"
     CDS             <1..428
                     /locus_tag="PKV23_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020187035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HQX85492.1"
                     /translation="AASFVHAVTRLGGKLRVATPGPLSLSPDLMKWARSNGADITVTT
                     SADVAVAGATCVVTDAWVSMHDADAANRHNLLKPYQVNAALMKKAAENAIFMHCLPAH
                     RGEEVTSDVMDGPQSVVFDEAENRLHAQKAILLHCFGLT"
     gene            425..1390
                     /locus_tag="PKV23_08400"
     CDS             425..1390
                     /locus_tag="PKV23_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone"
                     /protein_id="HQX85493.1"
                     /translation="MTRAQDTVMPFEIKPLGVRGRVVRLGAVIDDIVQRHEYPPVVSA
                     LLAEAIALTAMLGATLKFKGKFILQTKTDGPVDMIVADFISPNGVRGYARFNRKKLDA
                     LSTPSQQDLLGKGYLAMTVDQGSDMERYQGIVPLDGTTLAAAADTYFKQSEQIPTRLR
                     LASGPLLARGEKTTHWRAGAIMVQHLPAEGGTSPIAFSSGDNPEGEEQVLEDDNWVKA
                     KLLLDTVEDHELLDPTLTTEELLYRLYHEDGVTVYSPSPIIRHCTCSPEAVQKMLLGF
                     SEEDRNDMIENGEIKVICEFCSTAYRFDPETLEPLTTGSAPATAP"
     gene            complement(1359..>2047)
                     /locus_tag="PKV23_08405"
     CDS             complement(1359..>2047)
                     /locus_tag="PKV23_08405"
                     /inference="COORDINATES: protein motif:HMM:NF019307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HQX85494.1"
                     /translation="KPRAVIVGEPTSMRVVNAHKAALTFSTEVTGHEAHSSLTDQGVN
                     AIMVAGELLGEINRIREDLTVRRDPSQRFNPPYSTIHVGVIEGGTAKNIIPRHCSFQW
                     ETRLLPLADPDEVPARFEKYAASLEPAMKKIAAGTGINNRQTNTVPGLVPEDNSPAEH
                     LALHLAGANGTHAVSYCTEAGLFQQIGIPAIICGPGSIEQAHKPDEYIDISEMQKCEV
                     FMERLLEHCR"
BASE COUNT          401 a          607 c          643 g          396 t
ORIGIN      
        1 ttgcggcctc attcgtgcac gcggtaacgc gcctgggagg caagctgcgg gtggccacgc
       61 caggcccgct ttcgctgagc cccgacctta tgaaatgggc gcgcagcaac ggggcggata
      121 tcacggtcac gacgtcggct gatgtggccg ttgccggcgc cacctgcgtg gtaaccgatg
      181 catgggtttc catgcatgac gccgatgctg ccaaccgcca caatctgctc aaaccctatc
      241 aggtcaatgc ggccctgatg aagaaggccg ccgaaaatgc catcttcatg cattgcctgc
      301 cggcgcaccg cggcgaggaa gtcacctccg acgtcatgga cgggccgcaa tcggtggtgt
      361 ttgacgaggc ggaaaaccgc ctgcacgcac agaaagccat tctcctgcat tgtttcggcc
      421 tgacatgaca cgcgcacagg acacggtcat gcccttcgag atcaagcctc ttggggttcg
      481 agggcgcgtt gtccggcttg gcgcagtgat cgatgacatt gtacaacgcc acgaatatcc
      541 gccagtggtt tcggccttgc ttgccgaggc gatagcgctt accgcaatgc tcggcgccac
      601 cctgaagttc aagggcaaat tcatcctgca gacaaagacc gatggcccgg tcgacatgat
      661 cgtggcggat ttcatctcgc ccaacggggt gcgcggctat gcccgcttca accggaagaa
      721 gctggacgcc ctcagcacgc cctcccagca ggatttgctg ggcaagggct atctggccat
      781 gacggttgac cagggttcgg acatggagcg ctatcagggc atcgtgccgc ttgacgggac
      841 aacgcttgct gccgcggcgg atacctattt caaacagtcg gagcaaattc ccacccggct
      901 gcggctggcg tccgggccgc tcctggcgcg gggcgaaaaa accacccatt ggcgggccgg
      961 ggcgatcatg gtgcagcatc tgccggccga gggcggaaca agccccatcg cgttttcatc
     1021 gggcgacaat ccggagggcg aagagcaggt tctagaagat gacaactggg tgaaagccaa
     1081 gctgctgctc gacacggttg aggaccacga gctgcttgat cctacgctca ccacggaaga
     1141 actgctgtac cggctctatc acgaggacgg agtaacggtt tattccccca gccccatcat
     1201 ccggcattgc acctgctctc cggaggcggt gcagaaaatg cttctcgggt tttccgaaga
     1261 agaccgcaac gacatgatcg agaatggcga gatcaaggtc atctgcgaat tctgctcgac
     1321 ggcctaccgc tttgatccgg agacactgga gccgctgact accggcagtg ctccagcaac
     1381 cgctccataa acacttcaca tttctgcatt tccgaaatat cgatgtattc atcgggcttg
     1441 tgggcctgtt cgatggagcc ggggccgcag atgatggcgg gaatgccgat ctgctggaag
     1501 aggccggcct cggtgcaata ggacactgca tgggtgccat tggcgccggc gagatggagc
     1561 gccagatgct cggccggcga attatcctcg ggcaccaggc ccggcacggt attggtttgg
     1621 cggttgttga tgcccgtgcc ggccgcgatt ttcttcatgg caggctcaag gctagcggca
     1681 tatttttcaa agcgggccgg cacttcatcg ggatcggcca gcggcaggag cctggtttcc
     1741 cactggaagg agcagtggcg cgggatgatg ttcttggcgg tgccgccttc gatgacgccc
     1801 acatggatgg ttgaataggg cgggttgaag cgctgcgagg gatcgcggcg caccgtcaaa
     1861 tcctcgcgga tgcggttgat ctcgcccaaa agctcgcctg ccaccatgat ggcgttcacg
     1921 ccctggtcgg taaggctcga atgggcctcg tggcctgtca cttcggtgga gaaggtgaga
     1981 gccgccttgt gggcattcac cacccgcatg gaggtgggct cgcccacaat caccgcccga
     2041 ggctttt
//
LOCUS       DAORVN010000416         2040 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_59021_length_2040_cov_3.061461, whole genome shotgun sequence.
ACCESSION   DAORVN010000416 DAORVN010000000
VERSION     DAORVN010000416.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59021_length_2040_cov_3.061461"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1117
                     /locus_tag="PKV23_08410"
     CDS             <1..1117
                     /locus_tag="PKV23_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018045319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HQX85495.1"
                     /translation="LAYSTIPALEKKLKVAEEAGDSSMLEEAVTESHVAQVVSRWTGI
                     PVDKMLEGEREKLLQMEVELGKRVVGQGEAVEAVATAVRRARAGLQDPNRPIGSFMFL
                     GPTGVGKTELTKALANYLFDDEHALVRIDMSEYMEKHSVSRLIGAPPGYVGYDEGGAL
                     TEAVRRRPYQVVLFDEIEKAHPDVFNVLLQVLDDGRLTDGQGRTVDFKNTLIIMTSNL
                     GSEYLVALGEKQDVEEVREQVMGVVRGAFRPEFLNRLDEIILFHRLKRSHMGAIVDIQ
                     LLRLRKLLADRKIEVVLTEEARGLLADKGYDPAYGARPLKRVIQKSIQDPLAEEILAG
                     RVKDGDTVEVGVASGKFTFNGLVMGDKPKISNVVTLH"
     gene            1136..1498
                     /locus_tag="PKV23_08415"
     CDS             1136..1498
                     /locus_tag="PKV23_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011665748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HQX85496.1"
                     /translation="MKKLPRAAIKIDFGTEVRLGSGKVRLLELIAETGSISAAARQME
                     MSYRRAWLLIDEVNGIFGNPVVETVTGGTGGGGAKITPLGEAVIAAFRDIENNATELV
                     QTKLAKLLTQRGRGRREA"
     gene            1488..1853
                     /locus_tag="PKV23_08420"
     CDS             1488..1853
                     /locus_tag="PKV23_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015315606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HQX85497.1"
                     /translation="MKLEAYNAFCAKLPHATHVVQWGGAHVWKVGGKVFAVAGWNEGK
                     SLAVTFKCSQMSFNLLKEQEGLRPAPYLASRGMLWIQRTSSESMNDAALKDYLRESHR
                     LASLNLPKKTQRALGLNSA"
     gene            complement(1845..>2040)
                     /locus_tag="PKV23_08425"
     CDS             complement(1845..>2040)
                     /locus_tag="PKV23_08425"
                     /inference="COORDINATES: protein motif:HMM:NF015424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HQX85498.1"
                     /translation="KGIIARFDVDSGPTAIVHVKLEGGEELSAALTRKAMDQLGLDAG
                     QAVWCLLKSVSIDERWIASG"
BASE COUNT          416 a          559 c          678 g          387 t
ORIGIN      
        1 gcttgcctat tccaccattc ccgcactgga gaagaaactg aaggtggcgg aagaagcggg
       61 cgactcgagc atgctggaag aagcggtgac cgagtcccat gtggcccagg tggtttcacg
      121 ctggaccggc attccggtgg acaagatgct ggaaggcgag cgcgaaaaac tgctccagat
      181 ggaagtggaa ctgggcaagc gggtggtcgg ccagggcgaa gccgtagagg ccgtcgccac
      241 ggccgtgcgc cgcgccaggg cgggcttgca ggaccccaac cggcccatag gctcattcat
      301 gtttttgggc cctacgggcg tcggcaagac cgagctgacc aaggcattgg cgaattacct
      361 gtttgacgac gagcatgccc tggtgcgcat cgacatgtcg gaatacatgg agaagcattc
      421 ggtgtcgcgg ctcattggcg cgcctccggg ctatgtgggc tatgacgagg gcggggcgct
      481 caccgaagcg gtgcggcgca ggccctatca ggttgtgctg tttgatgaaa tcgagaaggc
      541 ccaccccgat gtcttcaacg tgctgctgca ggtgcttgat gacgggcgcc tgaccgacgg
      601 ccaggggcgc acggttgatt tcaagaacac gctgatcatc atgacctcga acctgggctc
      661 tgaatacctt gtcgcacttg gcgaaaaaca ggatgtggaa gaggtgcgcg agcaggtgat
      721 gggcgtggtg cgcggtgcct tccggccgga gttcctcaac cggctggacg agatcatcct
      781 gttccaccgc ctgaagcgga gccatatggg ggccattgtc gatatccagt tgctgcggct
      841 caggaagctg ctggcggacc ggaagatcga agtggtgctg accgaagagg cgcgcgggct
      901 tctggctgac aaaggttatg atcccgccta tggggcgcgg ccgctgaagc gggtgatcca
      961 gaaatccatt caggatccgc tggccgaaga aatcctggcg ggccgggtga aggatggcga
     1021 cacagtcgaa gtcggcgtag cctcgggcaa gttcaccttc aatgggctgg tgatgggtga
     1081 caagcccaag atcagcaatg tggtgaccct gcactgatgt gctagcatgg gggccatgaa
     1141 gaaactcccg cgcgccgcca tcaagattga tttcgggacc gaggtccgcc tcggttccgg
     1201 caaggtgcgc ctgctggaac tcattgccga gaccggctcc atttcggcag cggcccggca
     1261 gatggaaatg tcctatcgcc gggcctggct gctgatcgat gaagtcaacg gcatttttgg
     1321 aaatccggtg gttgaaacgg tgaccggcgg aactggcggc ggcggagcca agatcacgcc
     1381 gcttggcgaa gcggtcattg ccgcgttccg cgatattgaa aacaatgcca cggagctggt
     1441 tcaaaccaag ctggccaagc ttctgactca gcgtggacgc gggcgccgtg aagcttgagg
     1501 cctataatgc cttttgcgcc aagctccccc atgccaccca tgtggttcaa tggggcgggg
     1561 cccatgtctg gaaagtgggt ggcaaggtct ttgccgtcgc cggctggaac gagggcaaat
     1621 ccctggcggt caccttcaaa tgctcacaga tgagtttcaa cctgttgaag gaacaggagg
     1681 gcctgcgccc ggcgccttat ctggcctcgc gtggcatgct gtggatccaa cgcacgtctt
     1741 cggaaagcat gaatgatgcg gcgctgaagg attatctgcg cgagagccac aggctggcgt
     1801 cgctcaatct gccgaagaaa acccagcggg cactgggcct caattcagcc tgaggcaatc
     1861 cagcgctcgt cgatggatac ggatttgagc aggcaccaga cggcctggcc ggcatcgagg
     1921 cccaattgat ccatggcctt gcgggtgagt gctgcagaca attcttcgcc gccttccagt
     1981 ttgacatgca cgatggcggt gggaccgcta tcaacatcga atctggcgat gatgcctttc
//
LOCUS       DAORVN010000417         2038 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_59126_length_2038_cov_2.977307, whole genome shotgun sequence.
ACCESSION   DAORVN010000417 DAORVN010000000
VERSION     DAORVN010000417.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59126_length_2038_cov_2.977307"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            50..949
                     /locus_tag="PKV23_08430"
     CDS             50..949
                     /locus_tag="PKV23_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009469761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline ABC transporter substrate-binding
                     protein"
                     /protein_id="HQX85499.1"
                     /translation="MATAGMAADAASCQKVRLSDVGWTDITATTALTSAILEQMGYQG
                     DISQLAVPVTYASMKNKDIDVFLGNWMPSMENDIKAYREDGSVETLGANLEGAKYTLA
                     VTQGAYDAGLKDFADIGKFKDKLGGKIYGIEPGNDGNRLVLEVIKDPKFGLDGFELVE
                     SSEAGMLAQVAKSSAAGEDVVFLGWAPHPMNSNFKISYLTGGDDYFGPDFGGATVYTN
                     VRQGYLTECPNVGKFVSNLKFSMDMENQIMGEILDKGADPKAAGIAWIKANPDALTAW
                     LDGVTTFDGSGDALSAVKKGLGL"
     gene            1053..1907
                     /gene="choW"
                     /locus_tag="PKV23_08435"
     CDS             1053..1907
                     /gene="choW"
                     /locus_tag="PKV23_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007198606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline ABC transporter permease subunit"
                     /protein_id="HQX85500.1"
                     /translation="MEQIVNFLTGNKIPIGAWGKQFFTFLTTNFAWFFDSIARGLTFI
                     LESIVVLLLWFPPLVLVAAIAALAWYLQKSWRLAAVTALGLLFIINQGLWKETVETIV
                     LVVAATSASMAIGVPIGIWAAHNERIYKYLHPVLDLMQTMPTFVYLIPVLILFGLGVA
                     PGLIVTVIFAAPAPIRLTHLGITSVPKALLEAGEAFGATKRQLLWKVELPSALPTIMA
                     GLTQCIMLSLSMVVIAALIGASGLGKPVVRGLNSLNIPLGIEAGLAIVILAIILDRMC
                     RVSTGAKS"
BASE COUNT          396 a          598 c          626 g          418 t
ORIGIN      
        1 cgtaaccaaa tttctgggcg ccgccacctt tggcgctggc cttttcgcca tggcgacagc
       61 gggcatggcc gccgacgcgg cgtcgtgcca gaaagtgcgc ctctcggatg tgggctggac
      121 cgatatcacc gccaccacgg cgctgacctc ggccattctc gagcagatgg gctaccaggg
      181 cgacatttcg cagctggcgg tgcctgtgac ctatgcctcg atgaagaaca aggacatcga
      241 cgtattcctc ggcaactgga tgccgtcgat ggagaacgac atcaaggcct accgcgagga
      301 cggctcggtt gaaacgctgg gcgccaatct cgagggggcg aagtacaccc ttgccgtgac
      361 ccagggcgcc tatgacgcgg gcctcaagga tttcgccgac attgggaaat tcaaggacaa
      421 gcttggcggc aaaatttacg gcattgaacc cggcaacgac ggcaaccgcc tggttctcga
      481 ggtgatcaag gatccgaaat tcggcctcga cggttttgaa ctggtggaaa gctcggaagc
      541 gggcatgctg gcgcaagtgg ccaagtcctc agcggctggc gaagacgtgg tgttcctggg
      601 ctgggcgccg catccgatga actccaactt caaaatctcc tacctcaccg gcggcgatga
      661 ctatttcggt ccggattttg gtggcgccac agtctatacc aacgtgcgcc agggctatct
      721 gacggaatgc ccaaatgtcg gaaagtttgt ttccaacctc aagttcagca tggacatgga
      781 aaaccagatc atgggcgaaa tcctggacaa gggcgctgac ccaaaggcgg cgggcattgc
      841 ctggatcaag gccaacccgg atgcgctcac cgcctggctt gacggtgtca ccaccttcga
      901 cggttcgggc gacgcgctca gcgccgtcaa aaaagggctt ggcctttaat atctgacgac
      961 tatgatgtga cacatacggc ggactggaag ataaaaatac gccagtccgc ctttcctttt
     1021 taagactcaa gctggggagg ggtcttttaa tcatggaaca gattgtcaat tttctgacgg
     1081 gcaacaaaat tccaatcggg gcgtggggca aacagttctt caccttcctg accacgaatt
     1141 tcgcgtggtt ctttgattca atcgcgcggg gcctgacttt catccttgaa agcatcgtgg
     1201 tgctgttgct gtggtttccg ccgctggtgc tggtggcggc gattgcggcg ctggcctggt
     1261 acctgcaaaa atcctggcgg ctggcggcgg tcaccgcact cgggctgctg ttcatcatca
     1321 accagggcct gtggaaggaa acggtcgaga ccatcgtgct ggtggtggcc gctacctcgg
     1381 cctcgatggc cattggcgtg ccgatcggca tctgggcggc gcataacgag cgcatttaca
     1441 agtacctgca tccagtgctt gacctgatgc agaccatgcc gaccttcgtc tacctcatcc
     1501 cggtgctgat cctgttcggc ctcggcgtgg cgccggggct catcgtgacg gtgatcttcg
     1561 cggcccctgc ccccatcagg ctgacgcatc tcggcatcac ctcggtgccc aaggccctgc
     1621 tcgaggcggg cgaagccttc ggcgccacca agcggcaact gctgtggaag gttgagctgc
     1681 catcggcgct gcccacgatc atggcgggcc ttacgcaatg catcatgctg tcgctgtcca
     1741 tggtggtcat cgcggcgctg attggcgcct cggggctggg caagccggtg gtgcgcgggc
     1801 tgaactcgct caacattccg ctgggcatcg aggcggggct ggcgatcgtc atcctcgcca
     1861 tcatcctcga ccgcatgtgc cgggtttcaa cgggagcaaa atcatgagca ttgccgttga
     1921 tttcaagaac gtcgacatca ttttcgggcg cgacacgcgc gaggccatcg ccatgctcga
     1981 cgcgggcaag tcgcgctcgg aaatcctgga aaagaccggc gcggttttgg gctgcgcc
//
LOCUS       DAORVN010000418         2036 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_59229_length_2036_cov_2.927814, whole genome shotgun sequence.
ACCESSION   DAORVN010000418 DAORVN010000000
VERSION     DAORVN010000418.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59229_length_2036_cov_2.927814"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1596)
                     /locus_tag="PKV23_08440"
     CDS             complement(<1..1596)
                     /locus_tag="PKV23_08440"
                     /EC_number="1.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011439467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HQX85501.1"
                     /translation="MIVFRDIFSPRLILLTLAVVGLATTLIATMFLNVSGSVAAIPIL
                     IFGVLTLLGIRDMTQTRHAILRNYPISGHIRFLLENIRPEIRQYFLETDKDGMPFPRD
                     KRAIVYQRAKRALDKRPFGTQYDVYDNRFEWMHHSMVPRPPSREAFRISVGGPNCTQP
                     YSASVFNISAMSYGSLSANAIRSLNRGAKIGGFAHDTGEGGVSPYHREFGGDIIWEIG
                     SGYFGCRNPDGTFSDERFAATAKLDQVKMIEIKLSQGAKPGHGGVLPAVKITPEIAAI
                     RGIEMGRDCISPPTHSAFSTPLELVAFIARLRKLSGGKPVGFKLCIGHHWEFMAIIKA
                     TLETGVAPDFIVIDGKEGGTGAAPLEFMDHIGTPLRDGLSFVHSALVGANLRDQVKIG
                     VSGKITSAFDMARVMAIGADWCNAARGFMFAVGCIQAQHCHTDKCPTGVSTQDPARQR
                     AIVVPDKAERVASFHRETIKALSDLIAAAGLSHPGDLRPSHFMQRAAPDRSISFEELY
                     HCMKPGELLNGSAPSAMQHAWQLA"
     gene            complement(1663..>2036)
                     /locus_tag="PKV23_08445"
     CDS             complement(1663..>2036)
                     /locus_tag="PKV23_08445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85502.1"
                     /translation="TISKVPGEILRVKSGDYRIESRFAAGNAIAVVDVRVSPGLMSAV
                     EIDHAAGLARLSYVGAPDAQVSWLVTDDQGDVLPAIDGLSASVVLKPGAYTAKARIGS
                     EFLSASFDIAAGQERDILLGN"
BASE COUNT          429 a          544 c          671 g          392 t
ORIGIN      
        1 agccaactgc caagcgtgct gcatggcaga cggcgcactg ccattgagca attccccggg
       61 cttcatgcaa tgataaagct cctcgaaact gattgaacga tcgggcgcgg cgcgctgcat
      121 gaagtggctt gggcggagat cgccgggatg ggaaaggcct gccgcagcaa tcaggtcgga
      181 cagcgctttg atggtctcgc ggtggaagct tgccacgcgc tcggccttgt cgggaacgac
      241 gatggcgcgc tggcgggcgg gatcctgggt tgaaacgccg gtggggcact tgtccgtatg
      301 acaatgctgg gcctggatgc agcccactgc aaacatgaag ccgcgcgccg cgttgcacca
      361 gtcggcgccg atggccatga cccgggccat gtcaaaagcc gaagtgattt taccggagac
      421 gccgatcttg acctggtcgc ggaggttagc gccgacgaga gcggaatgga caaagctcag
      481 gccatcgcgc aatggggtgc cgatgtggtc catgaactct agaggggctg cacccgtgcc
      541 gccctccttg ccgtcaatca cgatgaaatc cggggcgaca ccggtttcca gcgtggcttt
      601 gataatcgcc atgaattccc aatgatggcc gatgcagagc ttgaagccca caggcttgcc
      661 cccggacagc ttgcgcagcc tggcgataaa ggccacgagt tcaaggggtg tggaaaaggc
      721 ggaatgggtt ggcggcgaga tgcaatccct gcccatttca atgccgcgaa tggcggcaat
      781 ctccggggtg atcttcacgg cgggcagaac gccaccatga ccgggctttg ctccttgcga
      841 gagcttgatc tcgatcatct tgacctgatc gagctttgcg gtcgcggcga agcgctcgtc
      901 ggagaaagtt ccgtcagggt tgcgacagcc gaaatagccg gagccgattt cccagatgat
      961 gtcaccgccg aattcgcggt gatagggact tacgccacct tcgccggtgt catgggcaaa
     1021 gccgccgatt ttagcgcctc ggttgaggga acgaatggca ttggcggaaa gggaaccata
     1081 gctcatggct gagatattga agacggatgc tgaatagggc tgggtacagt tagggccgcc
     1141 cacggaaatg cggaacgcct ccctgcttgg cggcctgggg accatggaat ggtgcatcca
     1201 ttcaaaccgg ttatcgtaga catcgtattg ggtgccgaag gggcgcttgt cgagggcccg
     1261 cttggcgcgc tggtaaacga tggcgcgctt gtcacgcggg aagggcattc cgtccttgtc
     1321 ggtttcaaga aagtattggc ggatctccgg gcggatgttt tcaagcagga agcggatgtg
     1381 gccggaaatg gggtagttgc gcaggatggc gtggcgggtt tgggtcatgt ccctgatgcc
     1441 aagaagtgtc aggacgccaa agatcaggat aggtattgcg gccaccgatc cgctgacgtt
     1501 gagaaacatg gttgcaatta aggttgtggc caagcccaca acggcaaggg tgagcaagat
     1561 caagcgcgga ctgaaaatgt ctctgaaaac gatcactgta cgtcctcccc ggggaccatg
     1621 attcccggtc agtgtgacag ctttgcaaaa ctatggaagt gcctaatttc cgagcaggat
     1681 gtcgcgttcc tggcctgcgg caatgtcaaa gctcgcgctg aggaactcgc tgccgatccg
     1741 ggcctttgcg gtataggcgc cgggcttgag gacaacactg gccgacaatc cgtcaatggc
     1801 gggaagaaca tcgccctgat cgtcggtgac cagccaggag acctgggcat cgggagcgcc
     1861 tacatacgag agccgcgcca gtccggcggc gtggtcgatt tcaacggcgc tcatgaggcc
     1921 ggggctgacg cggacgtcca ccacggcgat ggcattgccc gccgcaaaac ggctttcaat
     1981 gcggtaatcg ccggatttta cgcggagaat ttcacccggc actttggaaa tggtga
//
LOCUS       DAORVN010000419         2036 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_59241_length_2036_cov_2.577991, whole genome shotgun sequence.
ACCESSION   DAORVN010000419 DAORVN010000000
VERSION     DAORVN010000419.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59241_length_2036_cov_2.577991"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..409
                     /locus_tag="PKV23_08450"
     CDS             <1..409
                     /locus_tag="PKV23_08450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85503.1"
                     /translation="GAKRKIGDAAKIDITVEDRSKPSAQRRALGAAIGQRHHFISNLS
                     DVAFGGVNRRQNGTNASRQPCSEKQGCKFPATELAGCFAVHRTSKQLDRVSSKRLLFT
                     AQYAPKVWKKQDRNALAAMHSETALPIHGLINH"
     gene            457..1461
                     /locus_tag="PKV23_08455"
     CDS             457..1461
                     /locus_tag="PKV23_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HQX85504.1"
                     /translation="MDELLVGLRAIAESTRVRILFVLSHGEFNVSELTQILGQSQPRV
                     SRHLKLMVEAGMVSRHKEGNWVLFRLREEGLGGTLSRAIVDMLPAHDATLARDLARLE
                     TIRTQRAELAASYFSSNAANWQKLRSLHIREEDVEGAMQAILGKARVKSLVDLGTGTG
                     RVLELFAPQAEQAIGIDSSRDMLSIARSNLEKKALRHAQVRQGEIYALPFANGSADLV
                     VIHQVLHYLDDPGQALLEARRILHPGGRLLVVDFAPHDLESLRSEHAHRRLGISTEQI
                     TGWFNRAGLALQRHELLPPPWLKEQTGLTVSLWLGVPKTTKALQTPAENSITMESKAQ
                     "
     gene            1458..>2036
                     /locus_tag="PKV23_08460"
     CDS             1458..>2036
                     /locus_tag="PKV23_08460"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HQX85505.1"
                     /translation="MTDPATEAKKLTVSFEFFPPKAGDAEDSFWRTLKRLEPINPNFV
                     SVTYGAGGTTRDRTLSTVKRIRNETKLKPVAHLTCVGASKDEVNAVVRENWDAGVRHI
                     LALRGDPPGGVGMRFEPHPQGYKNAADLVRGIRAIADFEVSVTAYPEKHPQSVSIEED
                     LAFMADKAEHGASRAISQFFFHNSFFLRLRDRI"
BASE COUNT          442 a          659 c          551 g          384 t
ORIGIN      
        1 aggggccaag cgcaaaattg gcgatgccgc gaagattgac ataacggttg aggaccgaag
       61 caaaccgtcc gcgcagcgcc gtgcccttgg cgccgctatt ggccaacgac atcacttcat
      121 cagcaatctt tccgacgtag ccttcggcgg ggtcaaccgc cgccaaaacg ggacgaacgc
      181 ctcccgccag ccctgctccg aaaagcaagg ctgcaaattc ccggcgactg aacttgcggg
      241 ctgttttgcc gtgcatagaa cctccaaaca gcttgatcga gtttcctcca agcgtctgct
      301 atttaccgca caatacgccc cgaaagtatg gaaaaagcaa gaccgcaatg cgctcgcagc
      361 aatgcattca gaaacagcct tgccgatcca tggcttaatc aatcattgaa aagcatataa
      421 agatatgttt atgtgtattt caagaggaga tttggcatgg acgaactgct tgtaggattg
      481 agggcaatcg ccgaatcgac ccgcgttcgc atcctttttg tcctgtccca cggcgaattc
      541 aacgtcagtg agctcaccca gattctcggc caaagccagc cccgtgtcag ccgccatctg
      601 aagctcatgg tcgaagcggg tatggtctcg cgccacaagg aaggcaattg ggtgctcttt
      661 cgcctgcgcg aggaaggtct ggggggcacc ctgtcacgcg ccatcgttga catgctgccg
      721 gcccatgatg ccaccctggc ccgcgatctc gcccgtcttg aaaccatcag gacccaaaga
      781 gccgaattgg ccgcaagcta tttcagcagc aacgccgcca actggcaaaa attgcgctcc
      841 ctgcatatcc gcgaggagga tgtcgagggc gccatgcagg ccatccttgg caaggcacgt
      901 gtcaaatcgc tggtggacct gggcaccggc accggccggg ttctggaact tttcgcaccc
      961 caggcggagc aggccatcgg catcgattcg agccgcgata tgctctccat cgccaggtcg
     1021 aatcttgaaa aaaaggcgct gcgccatgcc caggtccggc aaggagaaat ctatgccctg
     1081 ccctttgcca atggttcggc tgatctcgtc gtcattcacc aggttttgca ctaccttgac
     1141 gaccccggac aggccctgct cgaggcgcgc cgcatactgc atcccggcgg tcgcctgctc
     1201 gtggtcgatt tcgctcccca tgacctcgaa tccctgcgca gcgagcacgc ccaccgtcgc
     1261 cttggaatct ctaccgagca aatcaccggc tggttcaatc gtgcgggtct tgcattgcag
     1321 cgacacgaac tcctgccgcc tccctggctc aaggaacaaa ccgggctgac cgtgtccctt
     1381 tggttgggcg tgcccaagac aaccaaggcc ctgcagaccc cagcggaaaa ttccatcacg
     1441 atggagtcaa aggcccaatg accgatccag cgacagaggc caagaaactc accgtgtcat
     1501 tcgagttctt cccgcccaag gccggcgacg ccgaggactc cttctggcgc accttgaagc
     1561 gccttgaacc gatcaatccg aatttcgttt ccgtcaccta tggcgccggc ggcaccacgc
     1621 gcgaccgcac cctgagcacg gtgaagcgca ttcgcaacga gacaaagctc aagcccgtgg
     1681 cccatctcac ctgtgtgggc gcgtccaagg atgaagtgaa cgccgtggtg agagaaaatt
     1741 gggacgcggg cgtccgccac atactggcgc tccggggcga tccgccggga ggcgttggca
     1801 tgcgttttga accccatccg caaggctaca agaacgccgc cgatctggtc cgcggaatcc
     1861 gcgccatcgc cgacttcgaa gtctcggtga cagcctatcc cgagaagcac ccgcaatccg
     1921 tttcaatcga ggaagacctg gccttcatgg ccgacaaggc cgagcacggc gccagccgcg
     1981 ccatctcgca gttcttcttc cacaattcgt ttttcctgcg cctccgggac cgcatc
//
LOCUS       DAORVN010000420         2034 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_59366_length_2034_cov_2.516928, whole genome shotgun sequence.
ACCESSION   DAORVN010000420 DAORVN010000000
VERSION     DAORVN010000420.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59366_length_2034_cov_2.516928"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..900)
                     /locus_tag="PKV23_08465"
     CDS             complement(<1..900)
                     /locus_tag="PKV23_08465"
                     /inference="COORDINATES: protein motif:HMM:NF015418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HQX85506.1"
                     /translation="MALMPVEEARTRILAHAKPLAPENVPLAQALGRALAKPLNAKRD
                     QPPFNSSAMDGYALRSGDTMTEFKVIGTSAAGHAFNGKLKPGQAVRIFTGAPVPTTAD
                     AVVIQENTSVTGKSVTILQAARPGQNIRLRGLDFRKGETLFPAAIRLNARDIGLAAAM
                     NCHIISVRRKPVVAVIATGDELVLPGAKPRPDQIVSSNSNALAALVESFGATVINGGI
                     VRDDLRATERAILKMAKADIIVTTGGASVGDHDFVQEALKNAGVKIDFWKIAMRPGKP
                     FMYGRKGQQQVLGLPGNPVSALVC"
     gene            complement(903..1379)
                     /gene="moaC"
                     /locus_tag="PKV23_08470"
     CDS             complement(903..1379)
                     /gene="moaC"
                     /locus_tag="PKV23_08470"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007755066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HQX85507.1"
                     /translation="MNKLTHLDATGNAHMVDVSDKHVTSRSATARAQVTMLPETLALI
                     LGGTAKKGDVLATARIAGIMAAKKTHELIPLCHPLLITKVSVDFVADEKTSSVEVSAT
                     VRVEGKTGVEMEALTACSVAALTLYDMCKAVDRGMKITDLRLTEKTGGKSGSFTAE"
     gene            complement(1376..>2034)
                     /gene="trpC"
                     /locus_tag="PKV23_08475"
     CDS             complement(1376..>2034)
                     /gene="trpC"
                     /locus_tag="PKV23_08475"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009463312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HQX85508.1"
                     /translation="GKLASGHWALIAEIKKASPSKGLIREHFSPPELAKAYEEGGAAC
                     LSVLTDTPSFQGAPEFLALARKACSLPALRKDFMLDPYQVLEARVWGADCILLIMAMI
                     DDGLAHELEAVAQDWNLDVLIEVHDQPELDRALKLSSPLIGINNRNLHTFETTLETTM
                     MLAPRIPKDRMIISESGLFKPRDLARLSGVGVNAFLIGESLMRQDDVAAATRAILAKP
                     "
BASE COUNT          395 a          553 c          653 g          433 t
ORIGIN      
        1 acagacaagg gcggacacag ggtttcccgg caggcccaga acctgctgct ggcctttgcg
       61 gccatacatg aacggcttgc ccgggcgcat agctattttc caaaaatcta tctttacgcc
      121 ggcgtttttc aaggcctcct ggacaaaatc atgatcgcct acggaagcgc cgccggtggt
      181 gacgatgata tcggccttgg ccattttgag aatggcgcgc tcagtggctc tgagatcgtc
      241 ccggacaatt ccgccattga tgaccgttgc gccaaagctt tccaccaggg cggcgagggc
      301 attgctgttt gaagatacaa tctgatcggg gcgtggcttg gcgccgggca gaacaagttc
      361 atcgccagtg gcaataaccg ccacgaccgg ttttctccgt acactgatga tatggcaatt
      421 cattgccgca gcgaggccaa tatcgcgggc gttcaggcgg atcgcagcgg ggaacagggt
      481 ttcgcctttg cgaaaatcca ggccacggag gcgaatgttc tggccgggcc gcgcggcctg
      541 caaaatggtg acggacttgc cagtgaccga tgtgttttcc tgaatgacga ccgcgtcagc
      601 agttgttggc accggtgcac cggtgaaaat tcgaacagcc tggcctggct tcaatttgcc
      661 attgaatgcg tgaccggcgg ctgaagtgcc aataaccttg aactcggtca tggtgtcgcc
      721 agaacgcaag gcgtagccat ccatggcgga ggaattgaac gggggctggt cgcgtttggc
      781 gttgagaggt tttgccaacg cgcgtccgag cgcctgagcc aggggaacat tctcgggggc
      841 cagcggcttt gcatgggcga gaatgcgggt gcgggcctct tcaacgggca tgagagccat
      901 ggctattccg ccgtaaagct gccggacttg ccgccggttt tttcagtgag acgcaggtcg
      961 gtgatcttca tgccgcggtc gacggccttg cacatgtcgt aaagggtcag ggcggcaacc
     1021 gagcaggcgg taagagcttc catctccact ccggtttttc cttcaacccg gacggtggcg
     1081 ctgacttcga cggagctggt tttttcatcc gccacgaaat cgacggatac cttggtgatg
     1141 agcaggggat ggcagagcgg gatgagttcg tgggttttct tggcagccat gatcccggca
     1201 atccgtgcgg tggccaggac atcgcccttc tttgcggtgc cgcccaggat gagagcaagg
     1261 gtttccggca gcatggtgac ttgcgcccgg gcggtggcgg agcgagaagt gacgtgcttg
     1321 tccgacacgt ccaccatatg ggcgtttccg gtggcatcta ggtgcgtgag cttgttcatg
     1381 gcttggcgag tatagcacgg gttgcggcgg ccacatcatc ctgccgcatg aggctctcac
     1441 cgataaggaa ggcatttacc ccgacgcccg atagtctggc cagatcccgg ggcttgaaca
     1501 ggccgctttc gctgatgatc atccgatcct tcggaatgcg aggggcgagc atcatggttg
     1561 tctcgagtgt ggtctcaaag gtgtgaaggt tgcggttgtt gatgcctatg agcggcgacg
     1621 acaatttcag ggcccggtca agctcaggct ggtcgtgaac ttcgatgagg acatcgaggt
     1681 tccagtcctg cgccacagct tcgagctcat gggcgaggcc atcgtcgatc atcgccatga
     1741 tcagcagaat gcaatcggcg ccccagaccc gggcttccag cacctgatag gggtcaagca
     1801 tgaaatcctt gcggagggca gggagtgaac aggctttccg ggccaacgcc aggaattccg
     1861 gcgcaccctg aaatgatggc gtatcggtga gcacagaaag gcacgcggcc ccgccttcct
     1921 catatgcctt ggcaagctcg ggtgggctga aatgttcgcg gataaggccc ttggacgggc
     1981 tggccttctt gatttcggca atcagcgccc agtggcccga agcgagtttc cctt
//
LOCUS       DAORVN010000421         2034 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_59368_length_2034_cov_2.435068, whole genome shotgun sequence.
ACCESSION   DAORVN010000421 DAORVN010000000
VERSION     DAORVN010000421.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59368_length_2034_cov_2.435068"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /locus_tag="PKV23_08480"
     CDS             complement(<1..353)
                     /locus_tag="PKV23_08480"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HQX85509.1"
                     /translation="MAAAPLLDPNALELLRMLDRDQPGAFSQFVRMFVHEAPDLVRKM
                     EQAQQAGDVDELGKSAHYLRSAALAMGASQLVEVCHGLEHHDLTDLQGSEMAARFDQL
                     RRAVRDALILLLQEVP"
     gene            501..1049
                     /gene="ppa"
                     /locus_tag="PKV23_08485"
     CDS             501..1049
                     /gene="ppa"
                     /locus_tag="PKV23_08485"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007068688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HQX85510.1"
                     /translation="MALDLVATGRDVPHEINVVIEIPKDAEPVKYEVDKATGAIFVDR
                     ILSTPMRYPCNYGYVPHTLCGDGDPVDVLVILPLPLIPGAVIRCRPVGMLKMKDEAGE
                     DTKLIAVPIDKVFGGYSHIHDIEQVSPHWLERIGHFFEHYKDLEKGKWVKLDGWEGAD
                     AAKREIVDSVQRFEASPEKLNF"
     gene            complement(1262..1789)
                     /locus_tag="PKV23_08490"
     CDS             complement(1262..1789)
                     /locus_tag="PKV23_08490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85511.1"
                     /translation="MKHSLKFSPLIIFVLAITACQRSELHMEVETRGDKLEIVLTNQL
                     SEPLAHSATVYSGLLAPDQDLILVFERDSRLHAQCVRLDQVPMGRDSLAVDATLRFSA
                     TLSQLSSLYCLEPGEKYFLRAALLERDGQAYRIVALSNRHPVVAVAGWRGQPSTSMSV
                     STDGRHIEYGPPVGR"
     gene            complement(1790..>2034)
                     /locus_tag="PKV23_08495"
     CDS             complement(1790..>2034)
                     /locus_tag="PKV23_08495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85512.1"
                     /translation="KLHATLHHEAGHDSTPAIQDGSYSDYYYEEAEMYQLNIDNADEY
                     GSTPSEREFFETERDSALENAKKWEAHEKRVESEDD"
BASE COUNT          425 a          670 c          571 g          368 t
ORIGIN      
        1 ggcacttcct gcagcagcag gatcagcgcg tcgcgcaccg cgcggcgcag ctggtcgaag
       61 cgggcggcca tttcggagcc ctgcaggtcg gtcaggtcgt ggtgctccag gccgtggcac
      121 acctccacaa gctggctggc gcccatggcc agcgcggccg agcgcaggta gtgggcggac
      181 ttgccgagtt cgtccacgtc gccggcctgc tgggcctgct ccatcttgcg caccaggtcc
      241 ggcgcctcgt gcacgaacat gcgcacgaac tggctgaacg cgcccggctg gtcgcggtcg
      301 agcatgcgaa gcagttccag ggcattggga tcaagcaggg gcgcagcagc catggcgggg
      361 gtttccgatg gaccagtccc cattctcgcc cctcccccca cccctgccta gcccccggct
      421 ggagtgcggg ctccagccca atcccgtatc cttcacgccc cacacggacg ccctcccccg
      481 ggctgcacag gagtatcccc atggcccttg acctggtcgc caccggccgc gacgttcccc
      541 acgaaatcaa cgtcgtgatc gaaatcccca aggacgccga gcccgtcaag tacgaagtgg
      601 acaaggccac cggcgccatc ttcgtcgacc gcatcctgtc caccccgatg cgctacccct
      661 gcaactacgg ctacgtgccg cacaccctgt gcggcgacgg cgacccggtg gacgtgctgg
      721 tcatcctgcc cctgcccctg atccccggcg ccgtcatccg ctgccgcccg gtcggcatgc
      781 tcaagatgaa ggacgaagcc ggcgaagaca ccaagctgat cgccgtgccg atcgacaagg
      841 tgttcggcgg ctattcgcac atccacgaca tcgagcaggt cagcccgcac tggctggagc
      901 gcatcggcca cttcttcgag cactacaagg acctggagaa gggcaagtgg gtgaagctcg
      961 acggctggga aggcgccgac gccgccaagc gcgaaatcgt cgattcggtg cagcggttcg
     1021 aggccagccc cgagaagctc aacttctgag acaactcgga gcgggagcca cgcaaaagcc
     1081 accctcgcgg gtggcttttt catttgaggg cttcgcagtc tcaacttgcc ctgacatccg
     1141 aattcgaagc aacacggcag ttcagacttt caggccataa cgtacataga aggccacagc
     1201 ccattcatgc aaaccgcccg atcagaaaca ggagcatcaa caagatacaa aaccttcccc
     1261 ctcaccttcc gacgggcgga ccatactcaa tatgtctgcc gtcagtcgag acactcatgg
     1321 acgtgctcgg ctgtccacgc cagcctgcga ccgcgaccac cggatggcga ttgctgagag
     1381 cgacgattct gtacgcctga ccatcccgtt cgagaagcgc ggcacggaga aagtacttct
     1441 cgcctggctc aaggcagtag agcgaagaca actggctcaa cgttgctgaa aaccgcaacg
     1501 tggcgtcgac cgccaagctg tcccgcccca tcggcacttg atcaaggcgg acacactggg
     1561 catgaagtcg actgtcgcgc tcaaatacga gaatcaaatc ttgatccgga gcgagcaagc
     1621 ccgagtagac cgttgcagaa tgcgccagag gctcggaaag ttggttcgtc agcacaattt
     1681 caagcttgtc gccgcgcgtt tcaacctcca tatgcagttc agaccgctga caggcggtga
     1741 tcgccaagac gaagatgatc aaaggagaaa atttgagtga gtgcttcatc tagtcatcct
     1801 cgctttcgac tcttttctca tgagcttccc actttttggc attctcaagg gcactgtctc
     1861 gctcagtctc gaagaactcc cgctcggagg gagtgcttcc gtactcgtca gcattgtcaa
     1921 tattcagctg atacatttca gcctcctcat agtagtaatc actataggat ccgtcctgta
     1981 tagcaggggt actgtcatga cccgcctcat gatgaagcgt cgcatgaagc ttcg
//
LOCUS       DAORVN010000422         2033 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_59414_length_2033_cov_2.774520, whole genome shotgun sequence.
ACCESSION   DAORVN010000422 DAORVN010000000
VERSION     DAORVN010000422.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59414_length_2033_cov_2.774520"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            31..1041
                     /locus_tag="PKV23_08500"
     CDS             31..1041
                     /locus_tag="PKV23_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018698298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HQX85513.1"
                     /translation="MKKLILSLLAAVLFAASAHATTLEDVKARGKLLCGVNPGLPGFA
                     DKGDDGAWVGFDVDFCRAVAAAVLGDAAQVDYVALTAQDRFEKLASGAVDVLARNTTW
                     TMERETKFPLRFAGVNYMDGQGFLVARALGVNSVFTMSQAAICFLTGTTTEANVADFF
                     RERQMVFTPVNFDSVNKVVAAYEAGQCDSYTADQSQLYAIRLKLKAPDDHIILPDVIS
                     KEPLGPMVRQGDEQWFNIVRWTLFALVNAEELDVKAASVEDLKASSKIPAVRRLLGLE
                     GTFGVDLGLDADWAARAIRATGNYAEIFERNLGKDSPLGIERGINALWSAGGLLYVPP
                     VR"
     gene            complement(927..1649)
                     /locus_tag="PKV23_08505"
     CDS             complement(927..1649)
                     /locus_tag="PKV23_08505"
                     /inference="COORDINATES: protein motif:HMM:NF015527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase family 2 domain-containing
                     protein"
                     /protein_id="HQX85514.1"
                     /translation="MPVFVINGKKILFIHVPKTGGTTIERVLSQHGPMSLHSNGSCFR
                     DQFRGGWLSKPVPLQHLHGALLGRLLLPCQFDLVFMLARDPVQRMISEYRHARELRRP
                     EALLSFSAWLRICLNLASIDPSFRNNHFRAQADYHVEGAKIFHFEDGIIPCLDEISAN
                     LNLPKFESLPHARRSKSFAAEPSSRDVELIQEAFAADYGLFPRYRTGGTYKSPPALHS
                     ALMPRSMPSGESLPRLRSNISA"
     gene            complement(1764..>2033)
                     /locus_tag="PKV23_08510"
     CDS             complement(1764..>2033)
                     /locus_tag="PKV23_08510"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQX85515.1"
                     /translation="GGQPAGYFELVPREDNVMELEYFGIIPVFQGRGLGKWLLAEAIR
                     AAWAKEPDKLIVETNSLDGPRALPLYQKMGFTPYAQEPQVLIARD"
BASE COUNT          415 a          538 c          664 g          416 t
ORIGIN      
        1 gcgttcttga agcctgttgg aaccgcattc atgaaaaaac tgatcttgtc gttgctggct
       61 gccgtgctgt ttgcggcatc agcccatgca acaacccttg aagacgtgaa ggcgcggggc
      121 aaattgctgt gcggagtcaa tcctgggctg ccgggctttg cagacaaagg cgatgacggc
      181 gcctgggtcg gcttcgacgt ggatttctgc cgcgccgtcg cggcggccgt gctcggtgat
      241 gcggcgcagg tggactacgt cgcccttacg gcgcaggacc gttttgaaaa gctggcatcc
      301 ggcgctgtgg atgtgctggc gcgcaacacc acctggacaa tggagcgcga gacgaagttt
      361 cccctgcgct ttgcgggcgt gaattacatg gacgggcagg gcttcctggt cgccagggcg
      421 ctgggggtga attcggtgtt caccatgagc caggcggcga tctgctttct caccggaacc
      481 acgacggagg ccaatgtggc ggatttcttc cgcgagcggc agatggtgtt cacgccggtg
      541 aattttgaca gcgtcaacaa ggttgtggcc gcctatgagg ctggccaatg cgacagctac
      601 acggcggacc agtcgcagct ttatgcgata cgcctgaagc tcaaggcgcc cgatgaccac
      661 atcattctgc ctgacgtgat ttcgaaggaa ccgcttggcc ccatggtgcg ccagggcgat
      721 gagcagtggt ttaatatcgt gcgctggacc ctgtttgccc tggtgaacgc ggaagaactg
      781 gatgtgaaag cggccagcgt tgaagacctg aaagccagca gcaagatacc ggccgtgcgg
      841 cgcctgcttg gcctggaagg cacctttggt gtggatttgg gactcgatgc ggactgggcg
      901 gcgcgggcga tcagggccac gggcaattac gcggaaatat tcgagcgcaa tctcggcaag
      961 gactcgccgc tgggcattga gcggggcatc aacgcgctgt ggagcgcggg cgggctcttg
     1021 tacgtgccgc cggtgcggta gcgcgggaac agtccatagt cggctgcaaa ggcctcctga
     1081 atcagctcca cgtcgcgcga tgacggctcg gccgcgaagc tttttgaccg gcgcgcatgg
     1141 ggcagacttt cgaacttcgg gaggttgagg ttcgcactga tttcatcaag gcaagggata
     1201 atgccatctt caaaatggaa gatttttgcc ccctcgacat gatagtcggc ctgggcacgg
     1261 aaatgattgt tgcggaacga tggatcgata ctggccagat taaggcaaat gcggagccat
     1321 gccgaaaacg acagcagcgc ctcaggccgc cgcaattcgc gggcgtgacg gtattcagag
     1381 atcatgcgtt gcacggggtc gcgggcaagc atgaaaacca gatcgaattg gcagggcagc
     1441 agcagccgcc caagcagcgc gccatgcagg tgttgcagcg gtaccggttt ggacagccat
     1501 ccgccgcgga actgatccct gaaacaagaa ccattgctgt gcagggacat ggggccatgc
     1561 tgtgaaagca cacgttcaat cgtagtcccg ccggttttgg ggacgtgaat gaagagaatt
     1621 ttttttccgt tgattacaaa tactggcatg aaacctgact gctgttcttc tattcgctga
     1681 agctaagcgg tgcagatgac aattggaaaa caatcagata acgattggct ctcgacccgg
     1741 caaaagctgc cttcattgac cgctcaatcg cgtgcgatca atacctgtgg ctcctgggcg
     1801 tagggggtga agcccatttt ctggtagagg gggagggccc gcggcccgtc caggctgttg
     1861 gtttcaacga taagcttgtc gggctctttg gcccaggcgg cgcggatggc ttcggcgagc
     1921 agccatttgc cgaggccgcg gccctgaaaa accgggatta tgccgaagta ttcgagttcc
     1981 atgacgttgt cttcacgcgg taccagttcg aaatagccgg cgggctggcc gcc
//
LOCUS       DAORVN010000423         2032 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_59462_length_2032_cov_3.191199, whole genome shotgun sequence.
ACCESSION   DAORVN010000423 DAORVN010000000
VERSION     DAORVN010000423.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59462_length_2032_cov_3.191199"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..153)
                     /locus_tag="PKV23_08515"
     CDS             complement(<1..153)
                     /locus_tag="PKV23_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013850273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HQX85516.1"
                     /translation="MKIGHRHYHTIWLNPDGRSVDIIDQRWLPHEFRVVTLETVDDVA
                     TAIRDMW"
     gene            complement(150..1421)
                     /gene="mtnK"
                     /locus_tag="PKV23_08520"
     CDS             complement(150..1421)
                     /gene="mtnK"
                     /locus_tag="PKV23_08520"
                     /EC_number="2.7.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018900952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046522 - S-methyl-5-thioribose kinase
                     activity [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose kinase"
                     /protein_id="HQX85517.1"
                     /translation="MNSYKDFIALSPETLPARLGGLAALKQRIGADAKAWKTREVGDG
                     NLNLVFIVEGPKGAVVVKQALPYVRLVGDSWPLPLKRSFFEYHALSRQAERDPGMVPE
                     IFHFDAGQAIIVMEFLSPHIILRRALIAGQELPKIAKDLGLFLARTLFRGSDLCLDTK
                     IRKADLALFADNVALCDITESLVFSDPYYDAKLNRHTAPQLDGIVKELRADRDLKVAA
                     QRLKHIFAATPETLLHGDLHTGSVMVTAEQTRVIDPEFAFYGPMAFDVGMLLANFWMS
                     YFSQSGHETAGNRASMRRYLLKVTVDIWEIFRDEFSRLWHGERTGILYQRSLFEDQGD
                     MLASEQALDLVLHGIWVDMLGFAGVEMHRRILGLAHNADFETIADADLRARCETKALK
                     LGRHLAVNRSHIHSILEVNKLAERLEKDIVT"
     gene            1521..>2032
                     /locus_tag="PKV23_08525"
     CDS             1521..>2032
                     /locus_tag="PKV23_08525"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HQX85518.1"
                     /translation="MKKPAGLSDNLRGILLLVASVTCFGVVDGISKMLIETQSFGQVM
                     LARYVLALPILLLATAPGQWKTLFHTKVVLLQIVRGIFPVVIGGSMVIAVWYLPLAEA
                     TVILFAGPFLVLALSGWLLGERVSAASWIGVVVGFIAVLIVARPGFSEFSQYTIFPLI
                     AAVFYAGFQLL"
BASE COUNT          371 a          572 c          658 g          431 t
ORIGIN      
        1 ccacatgtcg cggatggcgg tggcgacatc gtccacggtt tccagggtga cgacgcggaa
       61 ctcatggggc agccagcgct ggtcgatgat atcgacggag cggccatcgg ggttgagcca
      121 gatggtgtgg taatggcggt ggcctatttt cacgtgacga tatccttctc gagccgttca
      181 gccagtttat tgacttccaa tatgctgtga atgtggctgc ggttcaccgc caggtggcgg
      241 ccgagcttca acgccttggt ctcgcagcgg gcgcgcagat cggcgtcggc gatggtttca
      301 aaatcggcat tatgggcgag gcccagaatg cggcggtgca tctcgactcc ggcaaagccc
      361 agcatatcca cccagatgcc gtgcagcacc aggtcaagcg cctgctccga ggccagcatg
      421 tcgccctgat cctcgaacaa tgagcgctga tacaagatgc cggtgcgctc gccgtgccag
      481 agcctggaaa attcgtcgcg gaaaatttcc cagatgtcga cggtcacctt gagcagatag
      541 cgccgcatgg aggcgcggtt gccagcggtt tcatggccgg actgggagaa atagctcatc
      601 cagaaattgg ccagcagcat gccgacgtca aaggccatgg ggccgtagaa ggcgaattcg
      661 ggatcgatga cgcgggtctg ctccgccgtc accatcaccg aaccggtgtg gagatcgccg
      721 tgcaacagcg tttccggcgt ggcggcgaag atgtgtttga gcctctgggc cgccactttc
      781 aaatcgcggt cggcgcgcaa ttccttgacg atcccatcaa gctgcggcgc ggtgtggcgg
      841 ttgagcttcg cgtcataata gggatcactg aagaccaggc tttcggtgat gtcgcagagc
      901 gccacgttgt cggcgaagag cgccagatca gccttgcgaa ttttggtgtc gaggcagagg
      961 tcggagccgc gaaacagggt gcgggccagg aagagcccga ggtccttggc gattttcggc
     1021 agttcctgcc cggcaatcag ggcgcggcgc aggatgatgt ggggcgagag gaattccatg
     1081 acgatgatgg cttgcccagc atcgaaatgg aaaatctccg gcaccatgcc gggatcgcgt
     1141 tccgcttgcc tggacagggc gtggtattcg aagaacgagc gcttcagcgg cagcggccag
     1201 ctatcgccca cgaggcggac atagggcaac gcctgcttga cgacgacggc gcccttgggg
     1261 ccttcgacga tgaagaccag attgagattg ccgtcgccca cttcgcgcgt tttccaggcc
     1321 ttggcatcag caccgatccg ctgcttcagc gcggcaaggc cgcccagccg tgcgggcagt
     1381 gtttcggggc tcaaggcgat aaaatctttg tatgaattca ttttttccag catttggcag
     1441 gtgaaagagg ctaggttttg cacaatgccc gggcaaatgt aaatgacgcc gggcctcttg
     1501 tttgcgggac agaaccttcc atgaaaaaac cagccggcct gtcggataat ctgcgcggca
     1561 tcctgcttct ggtggcctcg gtcacctgct tcggcgtggt cgacgggatc agcaagatgc
     1621 tcatcgaaac ccaatccttc ggccaggtca tgctggcgcg ctatgtgctg gcgctcccca
     1681 tcctgctgct ggccacggcg ccgggccagt ggaagaccct gtttcatacc aaggtggtcc
     1741 tcctgcagat cgtccgcggc atctttccgg ttgtgatcgg cggatcgatg gtgatcgccg
     1801 tgtggtacct gccgctggcg gaagccacgg tcatcctgtt tgcggggccc tttctggtgc
     1861 ttgcgctttc cggttggctt ttgggcgaac gggtgagtgc ggcaagctgg atcggcgtcg
     1921 tggtgggttt cattgcggtt ctgatcgtgg cgcggccggg cttttccgaa ttttcgcagt
     1981 acacaatctt tccgctgatc gccgccgtgt tctacgcggg cttccaactg ct
//
LOCUS       DAORVN010000424         2030 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_59550_length_2030_cov_3.377722, whole genome shotgun sequence.
ACCESSION   DAORVN010000424 DAORVN010000000
VERSION     DAORVN010000424.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59550_length_2030_cov_3.377722"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..347
                     /gene="soxY"
                     /locus_tag="PKV23_08530"
     CDS             <1..347
                     /gene="soxY"
                     /locus_tag="PKV23_08530"
                     /inference="COORDINATES: protein motif:HMM:TIGR04488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thiosulfate oxidation carrier protein SoxY"
                     /protein_id="HQX85519.1"
                     /translation="EAKDMAAALKGIGAATPADSKDLILKVPDIAENGAVVPVEVISN
                     IPKTTSIAIMVEKNPQPLCANFEFANGALPEASARMKMGQTSMVKAVAKADGKFYTAQ
                     KEVKVTVGGCGG"
     gene            378..689
                     /gene="soxZ"
                     /locus_tag="PKV23_08535"
     CDS             378..689
                     /gene="soxZ"
                     /locus_tag="PKV23_08535"
                     /inference="COORDINATES: protein motif:HMM:TIGR04490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier complex protein
                     SoxZ"
                     /protein_id="HQX85520.1"
                     /translation="MADPMKIRAQLKGDVAEIRILMSHPMETGQRKDAAGATVPAHFI
                     QSMTVDVGGKRVVDGQIGTSVSRNPVFGFKMKGAKAGDKVVVNWVDNKGDKRTDEATV
                     A"
     gene            755..1618
                     /gene="soxA"
                     /locus_tag="PKV23_08540"
     CDS             755..1618
                     /gene="soxA"
                     /locus_tag="PKV23_08540"
                     /inference="COORDINATES: protein motif:HMM:TIGR04484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016669 - oxidoreductase activity, acting
                     on a sulfur group of donors, cytochrome as acceptor
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019417 - sulfur oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur oxidation c-type cytochrome SoxA"
                     /protein_id="HQX85521.1"
                     /translation="MNRNIISALCALTVVGGAFAQTPKKPSTKAAAAPTIEEKDAATL
                     EFEKFRAEMEESNPAELFEMKGQELWKTKRGPKNVSLAETCDLGQGIGKVDGAYVKMP
                     RYFADADAVMDAERRIVWCMVEKQGFKFDDIAKKPFQGGNDNSAPDITNLVTYVATHS
                     RNMKIAVPLNDPKVRDAYEVGKEIAAYRAGPYDFSCNTCHGGDGKRIRMQNLPNLSTP
                     QGALKGVQGWPGYRMTGGVMLTHQWRMGDCFRQQRFPQPKYASDAVAYLLTYMTGNAN
                     GQVYKGPGIKR"
     gene            1708..>2030
                     /locus_tag="PKV23_08545"
     CDS             1708..>2030
                     /locus_tag="PKV23_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011664491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85522.1"
                     /translation="MTKKMMLAIAMTAPLLIALPAAADGDHRAKAIAVIKKDFQPRGQ
                     AGIDRLAEDGVQAICNRTGNKPPEYIAKRLEADQLAGVKFPADGKLLGDWKSGEKIAQ
                     SGRGFT"
BASE COUNT          471 a          621 c          618 g          320 t
ORIGIN      
        1 tcgaagccaa ggatatggcg gccgcactga aaggcatcgg cgccgcgacg ccggccgaca
       61 gcaaggacct gatcctgaag gttcccgaca tcgctgaaaa cggtgctgtc gtgccggtgg
      121 aggtgatcag caacattccg aaaaccacgt cgattgccat catggtcgaa aaaaacccgc
      181 agccgctctg cgccaacttt gaattcgcca acggagccct gccggaggct tcggcgcgca
      241 tgaagatggg ccagacctcg atggtcaagg ctgtcgccaa ggccgacggg aagttctaca
      301 cggcgcaaaa ggaagtcaag gtcaccgtcg gcggctgtgg cggctgatcg aacagaacga
      361 acatagagga agacaccatg gcagatccga tgaaaattcg cgcccagttg aagggtgacg
      421 tggcggaaat ccgcatcctg atgagccacc cgatggagac cggccagcgc aaggacgcgg
      481 ccggtgccac cgtgccggcc catttcatcc agtcgatgac cgttgatgtc ggcggcaaac
      541 gggtggtcga cggacagatc ggcacctcgg tgtcgcgcaa tccggtattc ggcttcaaga
      601 tgaagggtgc caaggccggc gacaaggtcg tcgtgaactg ggtagacaac aagggcgaca
      661 agaggaccga tgaagccacc gtagcttgag cacatgatcg gcgaataaca atccgggcgg
      721 gctgcacctg cccaatcctt gacaggagga gtccatgaat cggaacatca tcagcgcgct
      781 ttgcgccttg acggtggtgg gtggcgcatt cgcccagacg ccgaagaagc cgtcaaccaa
      841 ggccgctgca gcgccgacga ttgaagaaaa agatgccgca accctggagt tcgaaaagtt
      901 ccgcgccgag atggaggaaa gcaatccggc cgaactcttc gaaatgaagg gtcaggaact
      961 gtggaagacc aaacgcggcc cgaagaatgt ctcgctcgcc gagacctgcg acctgggcca
     1021 gggcatcggc aaagtcgatg gcgcctacgt gaagatgccg cgctacttcg ccgatgcgga
     1081 tgcggtgatg gatgccgaac ggcgcatcgt ctggtgcatg gtcgaaaagc agggcttcaa
     1141 gttcgacgac attgccaaga agcctttcca gggcggcaac gacaatagcg ccccggacat
     1201 caccaatctg gttacctatg tcgccaccca ttcccgcaac atgaagatcg ccgtgccgct
     1261 caacgatccc aaggtgcgcg atgcgtatga agtcggcaag gaaatcgccg cctaccgtgc
     1321 cggcccctat gatttctcct gcaacacctg ccacgggggg gacggcaagc gtattcggat
     1381 gcagaacctg cccaacctca gcacacccca aggcgcgcta aagggtgtgc aaggctggcc
     1441 cggctatcgc atgaccggcg gcgtgatgct gactcatcag tggcgcatgg gcgactgctt
     1501 ccgccagcag cgcttccccc aacccaagta cgcctccgat gcggttgcct atctgctgac
     1561 ctacatgacc ggcaacgcca acggtcaggt ctacaaaggc ccgggcatca agcgctaaca
     1621 gggaccaaca tggcccccac gctcattcca ttcgctgccc ccacgggggg cacggcctcc
     1681 ttcgggacgg cccttcagga gtccgacatg accaagaaaa tgatgctggc catcgccatg
     1741 accgcccctc ttctgattgc cctgcccgcc gccgccgatg gcgatcatcg tgccaaggcc
     1801 atcgccgtga tcaagaagga tttccagccg cgcggtcagg cgggcatcga ccgtctggcg
     1861 gaagatggcg tacaggccat ctgtaaccgt accggcaaca agccgcccga atacatcgcc
     1921 aaacgactgg aagcggacca gttggcaggc gtcaagtttc ctgccgacgg caagctgctg
     1981 ggcgactgga aatccggtga gaagatcgcc cagagcgggc gcggtttcac
//
LOCUS       DAORVN010000425         2029 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_59598_length_2029_cov_3.540527, whole genome shotgun sequence.
ACCESSION   DAORVN010000425 DAORVN010000000
VERSION     DAORVN010000425.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59598_length_2029_cov_3.540527"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1336)
                     /locus_tag="PKV23_08550"
     CDS             complement(<1..1336)
                     /locus_tag="PKV23_08550"
                     /EC_number="3.5.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013168337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamate deiminase"
                     /protein_id="HQX85523.1"
                     /translation="MTQGKFRFTSALTSSGWQKNVAVAVDQNGMIESVAADAKDGALI
                     PGIAIPAVPNVHSHAHQRLMVGLAERAGPGADSFWTWREAMYGFALKLSPDDLEAVAA
                     QAYVEMVKAGFSCVGEFQYLHHKPDGSAYDEPAEMSLRCISAADEAGIAITMLPTLYN
                     YAGFGGQAPTAGQRRFVNGADRYLEIYETLKAVCGASPLRRIGISPHSLRAVTPELLK
                     TVLDGIDDDARVHIHVAEQSKEVDDCLAWCGKRPVEFLLDQFDVSGRWCTIHATHMTA
                     DETQRLAKSRAIAGLCPTTEANLGDGIFTADSFIKLGGAIAIGSDSQITISPAEDLRM
                     LEYSQRLRDRTRNALASGAGASTGRCLLDAVLKGGAACLDNPMGAIAAGKRCDIAVLD
                     RELSAMTGREEDAALDTWIFSAGNAAVKDVIVGGSHVVKDRRHIHEDRLQKRF"
     gene            1385..>2029
                     /locus_tag="PKV23_08555"
     CDS             1385..>2029
                     /locus_tag="PKV23_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018689032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HQX85524.1"
                     /translation="MLLSNLSIATMVGGYGLVEKGAVLIENDIIKSIGTDTTKAASID
                     CGGRLLTPGLIDCHTHLVYGGNRAAEFEQRLNGASYADIAKAGGGIMSTVHATRAASE
                     AELLESALRRLESLLAEGVTTIEIKSGYGLDVETEVKMLKVARKLGSLRPVDVKTTFL
                     GAHTFPQEFRETHEAYLKIVCDQALPRVAGEGLADAVDAFCEGIAFSVEETRTVF"
BASE COUNT          412 a          648 c          569 g          400 t
ORIGIN      
        1 ggaatctctt ttgaaggcgg tcttcgtgga tgtgcctgcg atccttcacc acatgcgagc
       61 cgcccacgat cacgtctttc accgccgcat tcccggcgga aaaaatccag gtgtcaagcg
      121 ccgcatcttc ctcgcgtccg gtcatcgcgg aaagctcacg atcaagcacc gcaatgtcgc
      181 agcgtttgcc cgcggcgatc gcgcccatgg ggttgtctag acaggcggcc ccgcccttga
      241 gcacggcatc taacaaacag cgcccggtcg aggccccggc cccggaggcc agcgcgttgc
      301 gcgtgcgatc acgcaggcgc tgcgaatatt ccagcatccg caagtcttcc gctggtgaaa
      361 tggtgatctg gctgtcactg ccgatggcaa tcgcgccgcc tagttttata aaactgtccg
      421 ccgtgaatat gccatccccg agatttgcct cggttgtggg gcacagtccg gcaatcgccc
      481 gggactttgc aagcctctgg gtttcatctg ccgtcatatg ggtggcatga atggtgcacc
      541 agcggccgga gacatcgaac tggtcaagca ggaattcgac cggccgtttg ccgcaccagg
      601 ccaggcaatc gtcgacttcc ttgctctgct cggccacatg gatatgaacc cgggcgtcat
      661 catcaatgcc atccaggacg gttttcagca gctccggcgt caccgcccgc aagctgtgcg
      721 gcgatattcc aatccgccgc aacgggcttg ccccgcatac ggctttcaag gtttcgtaga
      781 tctccaggta ccggtccgcg ccattgacaa agcgccgttg ccccgcagtc ggggcctgcc
      841 cgccaaagcc tgcgtaattg taaagcgtcg gcagcatggt gatcgcaatg cccgcctcgt
      901 ccgccgccga aatgcagcgc agcgacattt cagcgggttc atcgtaagcg ctgccatcgg
      961 gcttgtggtg cagatactgg aattctccca cgcaggaaaa gcccgccttc accatttcca
     1021 cataggcctg cgccgcaacc gcttcgagat cgtcgggcga gagcttgagc gcaaagccat
     1081 acatcgcctc gcgccaggtc cagaaactgt cggctcccgg ccccgcccgc tccgccagtc
     1141 caaccatcag tctttgatga gcatgcgagt gaacattggg aaccgcgggg atggcaatgc
     1201 ccggaatcag agcaccgtct ttcgcgtcgg ccgcaacact ttcaatcatc ccgttttgat
     1261 cgacggcgac agccacattc ttctgccagc cggaagatgt gagggccgaa gtgaaacgga
     1321 acttgccttg ggtcatgctt gcgagtatac tgtatataca acactataca agtagggaaa
     1381 cctcgtgctc ctgagcaatc tttccatcgc caccatggtg ggcggctacg gccttgttga
     1441 gaagggcgcg gtgctgatcg agaacgacat cataaaatcg ataggaaccg acactaccaa
     1501 agccgcctcg attgattgcg gcggcaggct cctcactccc ggcctcattg attgccacac
     1561 ccatctggtt tacggcggca accgcgccgc cgaattcgag caacggctga atggcgccag
     1621 ctacgccgac atcgccaaag ccggcggcgg catcatgtcc accgtgcacg ccacccgcgc
     1681 cgcctcggaa gcggagttgc tcgaatccgc attgcgtcga ctcgaatctc tcttggccga
     1741 aggcgtcaca actatcgaaa tcaagtcggg ctatggcctc gacgtcgaga ccgaggtgaa
     1801 aatgctcaag gtggccagaa agctgggcag cttgcgcccc gttgatgtga aaaccacctt
     1861 cctgggggcc cacaccttcc cgcaggagtt ccgggaaacc catgaagcct acctgaaaat
     1921 tgtctgcgat caggctcttc cgcgggttgc cggggaaggc ctcgccgatg ctgtcgacgc
     1981 tttctgcgaa ggcatcgcct tttccgttga ggaaacgaga accgtgttc
//
LOCUS       DAORVN010000426         2027 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_59698_length_2027_cov_3.321501, whole genome shotgun sequence.
ACCESSION   DAORVN010000426 DAORVN010000000
VERSION     DAORVN010000426.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2027
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59698_length_2027_cov_3.321501"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1224
                     /locus_tag="PKV23_08560"
     CDS             <1..1224
                     /locus_tag="PKV23_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012110875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HQX85525.1"
                     /translation="NTCPVGVATQDPELRKRFKGQPEHVINYFFFVAEELRELMAAMG
                     IRTYSELIGATEWLDQQEAISHWKAKGLDFSKVFAKADVQGPVATKKVSAQNHGIEHI
                     LDRRLIEQAMPALEKGTPVEIISKIRNADRTAGAMLSGEVAKRHGHAGLPEDTITVKL
                     NGVAGQSFGAWAAHGVTLELTGEANDYVGKGLSGGKLIVKPQEKIGFVPEKSIIVGNT
                     VLYGAITGECYFRGVAGERFAVRNSGAVAVVEGVGDHGCEYMTGGCVLVIGDTGRNFA
                     AGMSGGVAYVLDEAGDFAQRCNLAMVDLAPIPSEDATIERLHHHGGDLEHHGLVDVTA
                     AMDKHDGERIWELLSRHYKYTSSSRAKAIIDNWAQYLPKFVKIMPVEYARALSELEKA
                     QETSDGMTIGVKRSA"
     gene            1224..>2027
                     /locus_tag="PKV23_08565"
     CDS             1224..>2027
                     /locus_tag="PKV23_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008139023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HQX85526.1"
                     /translation="MGKVTGFLEIDRQERKYAPAADRVRHYNEFIIPLSDEGTRNQAA
                     RCMDCGIPYCHNGCPVNNQIPDWNDLVYNNRWEEASRNLHSTNNFPEFTGRICPAPCE
                     ASCTLNIDDNPVTIKSIECAIVDRAWSEGWLKPEIAAEKTGKKVAIVGGGPSGLAAAQ
                     QLARAGHNVHLFEKNRKAGGLMRYGIPDFKMEKHHIDRRVAQMEAEGVTFHYGAHIGV
                     TQDAQQLVDSFDAVLMTGGAEKPRDLPIPGRELAGIHPAMDFLPQQNRRV"
BASE COUNT          427 a          599 c          647 g          354 t
ORIGIN      
        1 aacacctgcc cggtcggcgt cgccacccag gacccggaat tgcgcaagcg cttcaagggc
       61 cagccggaac atgtgatcaa ctatttcttc tttgtcgccg aagagctgcg cgagctcatg
      121 gccgccatgg gcatcaggac ctacagcgaa ctgatcgggg ccacggaatg gctggaccag
      181 caggaggcga tcagccactg gaaggccaag ggcctcgatt tctccaaggt gtttgccaag
      241 gccgacgtgc agggaccggt tgcgaccaag aaagtctcgg cgcagaacca tggcatcgag
      301 catatcctcg accgcaggct tatcgaacag gcgatgcctg ccctggagaa aggaacgcct
      361 gtcgaaatca tctcgaagat caggaatgcg gaccgcacgg cgggcgccat gctctcgggc
      421 gaagtggcca aacgccatgg ccatgctggc ttgcctgaag acacgatcac ggtgaagctc
      481 aacggcgtgg cgggccagag ctttggcgcc tgggcggcgc atggtgtcac ccttgaactc
      541 acgggcgagg ccaatgacta tgtgggcaag gggctttccg gcggcaagct catcgtgaag
      601 ccgcaggaga agatcggctt cgttccggaa aaatccatca ttgtcggcaa cacggttctc
      661 tatggcgcca ttaccggcga atgctatttc cgcggcgtgg cgggcgagcg ctttgcggtg
      721 cgcaattcgg gcgccgttgc ggtcgtcgaa ggcgtcggcg accatggctg cgaatacatg
      781 accggcggct gcgttctggt catcggcgac acggggcgga atttcgccgc cggcatgtcg
      841 ggcggcgtgg cctatgttct cgatgaggcg ggcgacttcg cccagcgctg caatcttgcc
      901 atggtcgatc tcgcccccat tccttccgag gacgcaacga tcgagcgcct gcatcatcat
      961 ggcggcgatc tggagcatca cggactggtt gatgtgacgg cagccatgga caagcatgac
     1021 ggcgagcgca tctgggaact tctctcgcgc cactacaaat acacaagctc gtcaagggcc
     1081 aaggccatca tcgacaactg ggcgcagtac ctgccgaagt ttgtgaagat catgccggtt
     1141 gaatatgcgc gggccctttc ggaactggaa aaggcgcagg aaaccagcga cggcatgacc
     1201 atcggcgtca agaggagtgc atagtgggga aggttactgg atttctcgaa atcgaccggc
     1261 aggagcggaa atacgcgccg gctgccgacc gggtgcgcca ctacaatgaa ttcatcatcc
     1321 cgctgtcgga tgaaggcacc cgcaaccagg cggcgcgctg catggattgc ggcattccct
     1381 attgccacaa tggctgcccg gtgaacaacc agatcccgga ctggaatgac ctggtctaca
     1441 acaaccgctg ggaagaggct tcgcgcaacc tgcattccac caacaacttt cctgaattca
     1501 ccggacgcat ctgcccggcg ccgtgcgagg cctcgtgcac actcaacatc gatgacaatc
     1561 cggtcaccat caaatccatc gagtgcgcca tcgtggaccg ggcctggagc gaaggctggc
     1621 tgaagccgga gattgccgcg gagaagaccg gcaagaaggt ggccatcgtc ggcggcgggc
     1681 cgtctggcct ggcggcggcg cagcagctgg cgcgggccgg gcataatgtt catctctttg
     1741 agaagaaccg caaggcgggc ggcctgatgc gctacggcat tcccgatttc aaaatggaaa
     1801 agcaccacat cgaccggcgt gtggcgcaga tggaagcgga aggtgtgacc ttccactatg
     1861 gcgcgcatat cggtgtcacc caggatgccc agcagctggt tgacagtttt gatgcggtcc
     1921 tgatgacggg cggggcggaa aagccgcgcg atctgccgat cccgggccgc gagcttgcag
     1981 gcattcatcc cgccatggat ttcctgccgc agcagaaccg ccgcgtc
//
LOCUS       DAORVN010000427         2027 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_59700_length_2027_cov_3.244422, whole genome shotgun sequence.
ACCESSION   DAORVN010000427 DAORVN010000000
VERSION     DAORVN010000427.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2027
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59700_length_2027_cov_3.244422"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1340
                     /locus_tag="PKV23_08570"
     CDS             <1..1340
                     /locus_tag="PKV23_08570"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HQX85527.1"
                     /translation="ARQIRELVLSGRLRPQAKLPSTRGMAEDLNVARATVVEAYEQLL
                     SEGYIEARKGSGTRVAAELPEALLNSSPARLAAGKPRREKGREPARPFRSGLVDWEHF
                     PHDEWGRLLGRFWRNPPIAMLEHNDAFGWLPLREAIARHLYEWRGISCEASEVIMTAG
                     GMDAFDLINRSIFRAGDRIWMEEPGYPTARRVFSMGGVKPVAVPVDREGIMVVKGRQR
                     AGNARGAFVTPARQYPTGVTMPLSRRLELLDWADEAKAIIIEDDYDSEYRYVGRPLPA
                     LMSLDRKARVIYSGSFSKVFSPLLRLGYIVVPKALAERFRAERLAHGAPPSLMAQPAL
                     CEFMTSGAFAIHIRRMRRIYAARRKALIESLEGNPRFTIDASPSGLMLLLRLKSGEND
                     EEVVAKLASAHLEAQSLSSHYTGRQREQGLLLSFAGFTDKDLRAAAKKLVDEL"
     gene            complement(1357..>2027)
                     /locus_tag="PKV23_08575"
     CDS             complement(1357..>2027)
                     /locus_tag="PKV23_08575"
                     /inference="COORDINATES: protein motif:HMM:NF018420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1194 domain-containing protein"
                     /protein_id="HQX85528.1"
                     /translation="SRGLFALLALATPALAQETPVVIELVLALDSSASVDALEFDLQN
                     EGIARAFRDPEVLQAVDNLRPYGAAIAIVQWGGPGETQVVLPFTHIENARDAKAFGFR
                     VSLVRRWMRASSTSIATGINDARGLIENNGFDGQRKVIDVSGDGPDNSGDDLEGARQQ
                     ALASGITINGLSIEAEERALHDYYRARVIIGADSFVEPAEGFEDIARAMKEKLVRELR
                     PLGS"
BASE COUNT          385 a          630 c          624 g          388 t
ORIGIN      
        1 tagcccggca aatccgggaa ttggtgctga gcgggcgttt gcgcccacag gccaagctgc
       61 cgtcgacacg cggcatggcg gaggacttga atgtggcccg ggcgaccgtg gttgaagcct
      121 atgaacagct cctgagcgaa ggctatatcg aagcccgcaa aggctccggc acgcgcgttg
      181 ccgctgaatt gccggaagcg ttgctgaatt catctccggc gcgactggct gcgggcaagc
      241 cccggcgcga aaaaggccgc gaacccgcca ggccattccg ttcgggcctg gtggactggg
      301 agcatttccc gcatgacgaa tggggcaggt tgctgggccg cttctggcgc aatccgccaa
      361 ttgccatgct cgagcacaat gatgccttcg gctggctgcc gttgcgcgaa gccatcgccc
      421 ggcaccttta cgaatggcgc ggtatttcct gtgaggcatc cgaagtcatc atgacggcag
      481 gcggaatgga cgcctttgac ctgatcaacc gctccatctt cagggcgggc gaccggatct
      541 ggatggaaga gccgggctac ccaacggccc ggcgggtgtt ttccatgggc ggcgtaaagc
      601 ctgttgccgt gcccgttgac cgcgaaggaa tcatggtggt gaaaggccgc caaagggccg
      661 ggaacgcgcg cggcgcattc gtgacgccag cccggcagta tccaactggt gtaaccatgc
      721 cattgtcgcg gcggcttgaa ctgctcgact gggccgatga ggccaaggcc atcattatcg
      781 aggatgacta cgacagtgag taccgctacg tgggccgccc cttgccggcg ttgatgagcc
      841 tcgatcgcaa agcgcgggtg atttactccg gcagcttctc gaaagtgttt tcgccgctcc
      901 tgcgccttgg ctacattgtc gttccgaaag ctctggcgga acgcttccgt gccgagcgcc
      961 tggcccacgg cgcgccgcca tccctcatgg cccagcccgc cctgtgcgag ttcatgacgt
     1021 caggagcgtt cgccattcac atccgccgca tgcggaggat ctacgcggcg cggcgcaaag
     1081 ctctcatcga atcgcttgaa ggaaacccgc ggttcacgat tgacgcttcg ccttcaggtc
     1141 tcatgctttt actgcgcctg aagtccggtg agaatgatga ggaagtggtg gcaaaactcg
     1201 ccagcgccca cctggaagcg caatccctct ccagccacta cacgggcaga cagcgagagc
     1261 agggcctgct cctgagcttt gccggtttca ccgacaagga cctgcgggcg gctgcgaaga
     1321 aacttgtgga cgaactatag ttttgaaaga tgggtgctac gaccccagcg gccgcaactc
     1381 ccgcaccagc ttttccttca tcgcccgggc gatatcttca aagccttcgg ccggttcaac
     1441 aaatgaatcg gcgccaatga tgacgcgggc ccggtaatag tcgtgcaggg ctctttcttc
     1501 cgcttcaatg gagagcccgt tgatggtgat gccggaggcc agcgcctgct ggcgcgcccc
     1561 ttccaggtca tcgccgctat tgtcgggccc gtcgccggaa acatcgatca ccttgcgctg
     1621 tccgtcaaac ccattgttct cgataagccc acgggcatcg ttgatgccgg tggcgattga
     1681 tgtggagctg gcccgcatcc agcggcgcac caagcttacg cggaatccga aagctttcgc
     1741 atccctcgca ttctcgatgt gggtaaaagg aagcaccacc tgggtttcgc cgggtccgcc
     1801 ccactgcacg atggcaatcg ccgcgccata gggccggagg ttgtccacgg cttgcagtac
     1861 ttcgggatcg cggaatgccc gggcgatgcc ttcattctgc agatcaaact caagtgcgtc
     1921 gacacttgcg gaactgtcga gcgccagcac cagttcaatg actacggggg tttcctgcgc
     1981 caacgcaggc gttgccagcg ccagcagggc aaataaaccg cgcgacc
//
LOCUS       DAORVN010000428         2026 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_59762_length_2026_cov_2.717402, whole genome shotgun sequence.
ACCESSION   DAORVN010000428 DAORVN010000000
VERSION     DAORVN010000428.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59762_length_2026_cov_2.717402"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..650)
                     /locus_tag="PKV23_08580"
     CDS             complement(<1..650)
                     /locus_tag="PKV23_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008129839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HQX85529.1"
                     /translation="MSKPVKPPQDPNNIEPIQLREALEERYLAYALSTIMHRALPDAR
                     DGLKPVHRRILHVMRNLRLDPGSGFRKSAKIVGDVMGGFHPHGNVAIYDAMVRLAQDF
                     SSRYPLVDGQGNFGNVDGDSAAADRYTEARMTEVARLLLEHLDEDAVDFRPNYDGKEV
                     EPVVLPGAFPNLLANGATGIAVGMATSIPPHNAAELCDAALHLIKFPNAGVEKLTQ"
     gene            complement(667..>2026)
                     /locus_tag="PKV23_08585"
     CDS             complement(667..>2026)
                     /locus_tag="PKV23_08585"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HQX85530.1"
                     /translation="CTPFAAALLNNKLKEHGLDEDVKVQVIPLGGKFKVGSFDLEFVN
                     VTHSIPEPCALLIKTSHGTVFHSGDWKIDRTPTFGAGMDEKRLAEIGNEGVDVLICDS
                     TNVLREGFSPSESDVAATISDIVKHAKGRVAITTFASHVERIASAVRAARATGREVVL
                     AGRAMRNTIEAARACGYLRDAGEFLDEESFGYLPVNKIMLLCTGSQGEPRAAIARIGE
                     DQHPHVALEKGDLVIFSSRTIPGNEKEVSLVHNNLAKLDVDVITADDAMIHTSGHPRQ
                     GELKAMYEWLKPKALIPMHGEARHLRAHAKFARACGIPQAVVPLDGHMIRLCPGTLEN
                     VDEATFGRLHVDGKLIVPAEEGPAKQRRKLSAVGAVFVSLVLDEKLQLADDIQLLGDG
                     LPPGLEDDLLDAAEDAFESMPKPRRKDAVAVAETIRIAVRRAADQIWGKKPVCKVLVQ
                     RA"
BASE COUNT          378 a          574 c          663 g          411 t
ORIGIN      
        1 aactgggtga gtttctccac cccggcatta ggaaacttga tcaggtggag ggcggcatca
       61 cagagttcgg ctgcgttgtg cggcggaatt gaggtcgcca ttccgacggc aatgccggtt
      121 gcaccattgg ccaggagatt cgggaaggcg ccgggaagaa ccaccggctc cacttccttc
      181 ccgtcgtagt tcggccggaa atccaccgcg tcctcgtcga gatgctcaag aaggagcctc
      241 gccacttccg tcatgcgcgc ttcggtgtag cggtcggcag cggcgctatc gccatccacg
      301 ttgccgaaat tgccctgacc atcgacgagg ggatagcgcg acgagaagtc ctgggcgagg
      361 cgcaccatcg cgtcataaat ggcgacgttg ccgtgcggat gaaagccacc catgacatcg
      421 ccgacgatct ttgccgattt cctgaatccc gatccgggat cgaggcgaag gttccgcatg
      481 acatgcagga tgcggcggtg gacgggcttc agcccatcgc gcgcgtcggg cagcgcccgg
      541 tgcatgatgg tcgacagcgc ataggcgaga tagcgctctt ccagcgcttc gcgaagctgg
      601 atcggctcaa tgttgttggg gtcctggggg ggctttacgg gcttgctcat gattcgctgc
      661 agtatatcac gcgcgctgca cgagcacctt gcaaacgggc tttttgcccc atatttgatc
      721 ggcggcgcgg cgcaccgcta tgcggatggt ttcggccacg gcaacggcat ccttgcgtct
      781 tggcttcggc atggattcaa aggcatcctc ggccgcatcc agcaaatcat cttcaaggcc
      841 gggcggaagg ccatcgccga gcaattgaat gtcatcggcg agctgcagtt tttcatccag
      901 gacgagggac acaaaaaccg cgccgacggc ggacagtttg cggcgctgct tggcggggcc
      961 ttcttcggcc ggcacgatga gttttccatc gacatgcagg cggccaaagg ttgcttcgtc
     1021 gacattttcc agggtgccgg ggcagagccg gatcatatgg ccgtcaaggg gcaccacggc
     1081 ctggggaatg ccgcaggccc tggcgaactt cgcatgggcg cggagatggc gggcttcgcc
     1141 atgcatggga atcagcgcct tgggcttcaa ccactcatac atggccttga gttcaccctg
     1201 gcgcggatgg cccgaggtgt ggatcatggc atcatcagcg gtgatcacgt caacgtcgag
     1261 cttcgccaga ttgttgtgga caagggaaac ttccttttcg tttccgggaa tggtgcgcga
     1321 ggagaaaatg acgagatcgc ctttctcgag cgctacatgg ggatgctggt cttcgccgat
     1381 gcgggcgatg gcggcgcggg gttcaccctg gcttccggtg cacagcagca tgatcttgtt
     1441 gaccggcaga tagccgaagg attcctcatc aaggaattcg ccagcatcgc gcaggtagcc
     1501 gcaggcgcgg gcagcttcaa tggtgttgcg catggccctg cccgcgagca cgacttcacg
     1561 gcccgtggcc cgggcagcgc gcacggcgga agcgatgcgc tccacatggg aggcgaatgt
     1621 cgtaatggcg acgcggccct tggcgtgttt gacaatgtcg gagatggttg cggcgacatc
     1681 gctttcagat ggcgagaagc cttcacggag cacattggtg gaatcgcaga tcagcacatc
     1741 gacgccttcg ttgccgattt cggcgaggcg cttttcgtcc attccggcgc cgaaggtggg
     1801 ggtgcggtca attttccagt caccggaatg gaacacggtg ccatggctcg tcttgatcag
     1861 cagcgcgcag ggctccggaa tggagtgggt gacgttcaca aattccagat cgaaggagcc
     1921 caccttgaat tttccgccga gcggaatgac ttggaccttc acatcttcat cgaggccgtg
     1981 ttccttcagc ttgttgttga ggagcgccgc ggcgaagggc gtgcag
//
LOCUS       DAORVN010000429         2021 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_59989_length_2021_cov_3.750763, whole genome shotgun sequence.
ACCESSION   DAORVN010000429 DAORVN010000000
VERSION     DAORVN010000429.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2021
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59989_length_2021_cov_3.750763"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1862
                     /locus_tag="PKV23_08590"
     CDS             <1..1862
                     /locus_tag="PKV23_08590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85531.1"
                     /translation="KFATRLAAMLACLVLSNAAWAIDFTPHGTQPGLQFSLLNSDSCG
                     GCHGGFVGNDPNFRPHSTWSGSMMANATRDPLFWAALDIANLDVPGVGDYCLRCHTSQ
                     GWYGGRVVKPANDPVRGANGCLLEGTPDASDFSNDFSGVACHFCHRMMPTGPLGEPGM
                     IGNANAWLDDTECNGNEGEPCRRGPYGYTGSFQPPHAWAQSAYHEDSALCGSCHDVTT
                     PDTDVGPLKTLKLADGTDTTLPFPIERTYSEWKQSDFSEAGGQTCQGCHMPDSEDPTA
                     TACAGGPSRTGNLPVHTFVGGNTWVPGIIKGEYSDTSAIPGSAGGIGRQSALDRTVQA
                     ARELLQSSAEVDVSLISYTAPTINTAGALSARVKVTNLSGHKLPSGYGEGRRMWLNVQ
                     LLDFNGALVFESGAYDAPSALLASDPQLRVYEVLQGIWNRNGTGTCDAVDGLGREAFH
                     FALNDCVAKDSRIPPLGFQPATAADPNGYELRPVGATYAETSPGSGILVNFDEADYNV
                     VIPPGTPGPMSVSARLYYQTASREYIEFLRNQAIERAVPGENQLCSGEPNRPAVVGPK
                     NLSRGEYMWQLWNLPEAGSAERIFANNFDFSGYQSGYGKSPPESVDSDIALTP"
BASE COUNT          398 a          599 c          609 g          415 t
ORIGIN      
        1 tgaagttcgc aacgaggctg gctgcaatgc tggcttgcct tgtcctgtcg aacgccgcct
       61 gggccatcga cttcaccccg cacggcacgc agccaggttt gcagttttcg ctgttgaact
      121 cggattcctg tggcggttgc catggcggat ttgtcggcaa tgatccgaac tttcgtccgc
      181 acagcacctg gagcggttcg atgatggcca atgccacgcg tgatccgctc ttttgggcgg
      241 cgctggatat cgccaatctc gacgtccccg gcgtaggcga ctattgcctg cgatgtcata
      301 cctcgcaagg ctggtatgga gggcgggtgg tgaagccggc caatgatccg gtcaggggcg
      361 ccaatggttg cctgctcgaa ggcacgccgg atgcaagcga tttctcgaat gacttcagcg
      421 gtgttgcctg ccatttctgc catcgcatga tgcccaccgg tccattgggc gagccgggca
      481 tgataggcaa tgccaatgct tggctggacg acaccgagtg caatggcaat gagggtgagc
      541 cctgtcggcg gggtccctac gggtatacag gcagcttcca gccaccgcat gcctgggcgc
      601 aatcggcgta tcacgaagac agcgcgcttt gtggttcctg ccacgatgtc accacgcccg
      661 ataccgatgt cgggccgttg aaaacactca aactggccga tggaaccgat acgaccctgc
      721 cgtttccgat cgagcgcacc tacagcgaat ggaagcagag tgatttttcc gaggccggcg
      781 gccagacctg ccaaggctgc cacatgcccg acagcgaaga cccaacagcc acagcttgcg
      841 ctggcgggcc aagtcggacc ggcaacttgc ccgtgcatac cttcgtcggc ggcaatacct
      901 gggtacccgg catcatcaag ggcgaataca gcgatacctc ggccataccc ggctcggccg
      961 gtggcatcgg ccgccagagt gcacttgatc gaaccgtgca ggcagcgcgc gaacttctgc
     1021 aatcctccgc ggaagtcgat gtttccctga tttcctacac ggcgccaacc atcaataccg
     1081 caggtgctct gagcgcgcgg gtcaaggtca ccaatctgag cggccacaag ctgccgtccg
     1141 gatatggcga aggtcgtcgc atgtggctca atgtccagtt gctggatttc aacggggcgc
     1201 tggtgtttga aagcggcgcc tatgacgccc ccagcgcgct gcttgccagc gatccgcaat
     1261 tgcgcgttta cgaggtgctg cagggaatct ggaatcgcaa cggcacggga acctgtgatg
     1321 ccgtcgatgg gcttggccgg gaggcctttc atttcgcatt gaacgactgc gtggcaaagg
     1381 acagccgcat tccaccgctg ggtttccagc cggcaaccgc agccgatccc aatggctatg
     1441 agttgcgacc agtgggtgca acctatgcgg agacctcgcc gggcagtggc atcctggtca
     1501 atttcgacga ggccgactac aacgtggtga tcccgcccgg cacaccgggg cccatgagcg
     1561 tttcggcgcg gctctattac cagacggcaa gtcgggaata tatcgagttc ctgcgcaacc
     1621 aggccatcga gcgcgcggtc cctggggaaa accagttgtg ttcgggtgag ccgaaccgac
     1681 cagccgtggt cggtccgaaa aacctcagcc gcggtgagta catgtggcaa ttatggaatt
     1741 tgcccgaagc aggttccgcg gaacgcattt ttgccaacaa ttttgatttt tcgggttacc
     1801 agtcgggcta tggaaagtct ccgccggaat ctgtcgattc cgacatcgca ttgacgccat
     1861 gaaattgatt cgatacggca ttccggtcgg ccacccgcga aacgcggatg gcttccgatt
     1921 catctgcgat atccaatgca tggggatctg ttcatgaact tgaagttgat accggccatt
     1981 ctcttgattg catgcggtct gattacaccc tgggcgtccg a
//
LOCUS       DAORVN010000430         2020 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_60030_length_2020_cov_4.162850, whole genome shotgun sequence.
ACCESSION   DAORVN010000430 DAORVN010000000
VERSION     DAORVN010000430.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60030_length_2020_cov_4.162850"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..879)
                     /locus_tag="PKV23_08595"
     CDS             complement(<1..879)
                     /locus_tag="PKV23_08595"
                     /inference="COORDINATES: protein motif:HMM:NF024870.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HQX85532.1"
                     /translation="MAVTFRRAVDGDIGLIIGLAGASGSGKTYSAMRLAAGIAGNKPF
                     AVIDTEAGRARHYARQFAFDHADLRPPYRPLAYADALKAADDAGYPVIVVDSFSHEHA
                     GEGGILDWQEEELTRMAGTDWKRREACKMASWIKPKGDHKKMVSRLLKLQAHVILCFR
                     AEQKIEIVTQNGETKVVPKKIASGFSDWIPICEKNILYELTSSFLLTPDAPGIPKPIK
                     LQEQMRPFVNLNAPLDEQAGRKLAEWAKGGHDTQPPITASTHGLIADVRQAKAERSSP
                     AQALQDAAQELAGEPVE"
     gene            complement(889..1764)
                     /locus_tag="PKV23_08600"
     CDS             complement(889..1764)
                     /locus_tag="PKV23_08600"
                     /inference="COORDINATES: protein motif:HMM:NF024096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease-like domain-containing
                     protein"
                     /protein_id="HQX85533.1"
                     /translation="MDKAGIYNIALTDYINDPAPEPSLNASIATTLLTATPAHARLQH
                     PRLNPQQDREESSRLDLGTIAHGILLEGDSSRVVVIDADDWRKKETKEQRDEARAAGK
                     LPILRADHATVLEMVKAAEGYIYSSEIAADFKAAIPEQTLIWQEAGIWLRCRPDKATP
                     DWRVVFDYKTCAGTAHPAVWAKSSMVRYGYDIQAAINLRGVKALTDAKRTTFVFLVQE
                     IEPPYVLSIVSLSPEWLQLADDKLRVAMSIWQGCTRKNEWPGYPSRIAYVEPPAFASM
                     DWDACLPAIDAEDFI"
     gene            complement(1773..>2020)
                     /locus_tag="PKV23_08605"
     CDS             complement(1773..>2020)
                     /locus_tag="PKV23_08605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85534.1"
                     /translation="IRCRFGTFNKAKVAAGLPCTVKKRHSAHNLMRGLPPAPTSFYPC
                     WRCKKKYPGTGHKQGDYFCYACREQVNEMAANMAWLG"
BASE COUNT          372 a          629 c          554 g          465 t
ORIGIN      
        1 ttcgaccggc tcaccggcta gctcttgcgc ggcgtcttgg agggcttgag cggggcttga
       61 tcgttcagcc ttggcctgcc tcacatcggc aataagtcca tgggttgagg cggtgattgg
      121 aggctgcgtg tcatgtccgc ccttagccca ttcggccagc ttgcggcctg cctgctcgtc
      181 taatggggca ttgaggttca cgaacggacg catttgctct tgtagcttga tgggcttcgg
      241 gatgcctggc gcgtcaggcg tcaagagaaa cgatgaggtc aattcataca ggatattttt
      301 ctcgcaaata ggtatccaat cactgaaacc gctcgcaatc ttcttcggga ccactttagt
      361 ttccccgttc tgtgtcacaa tctctatttt ctgctcggcg cgaaagcaca ggatcacatg
      421 ggcttgcagt ttcagcagtc gagacaccat ctttttgtga tcgcccttgg gcttgatcca
      481 cgaggccatc ttgcacgcct cgcggcgttt ccagtccgtt cccgccatgc gggtcaattc
      541 ttcctcttgc cagtccagaa tgccgccctc tcctgcgtgc tcgtgcgaga acgaatccac
      601 cacaatgaca gggtagcctg catcgtccgc cgccttgagc gcatccgcat aggcgagcgg
      661 tctgtaagga ggccgcaggt cggcgtgatc gaaagcgaac tgcctagcgt aatgtctggc
      721 gcgtccggcc tcggtgtcga tcacggcgaa gggcttattg cccgcaatcc ctgcggccag
      781 tcgcatagcg gaataggttt tcccgctccc cgatgccccg gccagtccaa taatcagccc
      841 aatatctccg tcaacggccc gtcgaaatgt cacagccata attccctcct agataaaatc
      901 ttctgcatca attgccggta aacaggcgtc ccaatccatc gacgcgaagg ccggtggttc
      961 cacgtaggcg atgcgtgatg ggtagccagg ccactcattc ttccgcgtgc agccctgcca
     1021 aatcgacatg gcgacacgca gcttgtcatc agccagctgg agccattctg gcgaaagact
     1081 cacaatggac agcacatacg gcggctcgat ctcctgcacc agaaagacaa acgtggtccg
     1141 cttggcatcg gtcagcgcct tcacgccgcg taggttaatc gccgcctgga tgtcgtagcc
     1201 gtagcgcacc atggaggact tcgcccacac ggcagggtgt gccgtgcccg cacaggtttt
     1261 ataatcgaac accacgcgcc aatctggcgt ggctttgtcg ggtcggcagc gcagccagat
     1321 tccggcctct tgccagatca aggtttgttc tggaatggcg gctttgaagt cggcagcgat
     1381 ctcgcttgag tagatgtacc cttccgccgc cttgaccatt tccagcaccg tggcatgatc
     1441 agccctgagg atcggcagct tacccgccgc cctcgcctcg tcccgctgct ccttcgtttc
     1501 cttcttgcgc cagtcgtccg cgtcgatcac caccacgcgg gaactgtcgc cctccagcaa
     1561 gatcccatgc gcaatcgtgc cgaggtctaa ccggctgctt tcttcacggt cctgttgtgg
     1621 gttcaatcgc ggatgctgca gccgtgcatg ggccggtgtc gccgtcaaca acgtggtggc
     1681 aatactggcg tttaatgacg gctctggcgc tggatcgttg atatagtcgg tcaacgcaat
     1741 gttgtagatg cccgccttat ccatcgtccc ccctacccca accacgccat gttggcggcc
     1801 atttcgttga cctgctcacg gcaggcatag caaaagtagt cgccctgttt atgccccgtg
     1861 ccggggtact ttttcttgca ccgccagcag ggataaaaac ttgttggcgc aggtggtagg
     1921 ccacgcatca ggttatgcgc cgaatgccgc ttcttgaccg tgcatggcag gcccgccgcc
     1981 actttcgcct tgttgaacgt cccgaaccgg cagcgaatcg
//
LOCUS       DAORVN010000431         2018 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_60142_length_2018_cov_3.967906, whole genome shotgun sequence.
ACCESSION   DAORVN010000431 DAORVN010000000
VERSION     DAORVN010000431.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60142_length_2018_cov_3.967906"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..837)
                     /locus_tag="PKV23_08610"
     CDS             complement(<1..837)
                     /locus_tag="PKV23_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011975571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HQX85535.1"
                     /translation="MKKLLMTAAVAAVALGLGSTASLAEAYKACWIYVGPVGDGGWSF
                     QHDEGRKAVVAELGDKVETVFVENVAEPDSDRTIERLARDGCKIIFTTSFGFMEPTLK
                     VAAKFPDVKFEHATGYKTAPNVTTYNSKFHEGRYVIGQIAAKMSKTGTAGYIVSFPIP
                     EVIAGINAFMLGAQTVNPDFKVKIIWANTWFDPGKEADAAKVLLSQGADILVQHTDST
                     APLQIAQEQGKFGFGQASDMMAFAPKAQLTAIVDSWGPYYIKRVKAGMDGTWTSQQSW
                     DGM"
     gene            complement(921..1796)
                     /locus_tag="PKV23_08615"
     CDS             complement(921..1796)
                     /locus_tag="PKV23_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007197378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQX85536.1"
                     /translation="MDQSLAVMLTIITAATPLLIAAIGELVVERSGVLNLGVEGMMVM
                     GAVAGFAVAFMTGSTLLGVGAAIFAGIAMSALFGFLTLTLVANQVAAGLALTLLGLGL
                     SGLIGEAFVGQPGVKMPTLFGIDALVPFSLLLTAGVAYVLFRTKTGLVIRAVGNSHSS
                     AHALGYRVIAIRYLSVMFGGACAGLAGGYLSLAYTPQWIENMTAGRGWIALALVVFAS
                     WRPLRTLVGAYLFGAVSILGFVVQSMGLGIPSQFLSSLPYIVTIIALVIISGNKRLTQ
                     ANTPACLAQSFVPDR"
     gene            complement(1801..>2018)
                     /locus_tag="PKV23_08620"
     CDS             complement(1801..>2018)
                     /locus_tag="PKV23_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020049889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQX85537.1"
                     /translation="RLNPLGVLFAGLLLALSYIGGEAAQVTLGISDKIAKVFQGILLF
                     FILSCDTLILYRIKLEMGRVGALKAAE"
BASE COUNT          425 a          637 c          591 g          365 t
ORIGIN      
        1 catgccatcc caggattgct gcgaagtcca tgttccatcc atgccggcct tgacccgctt
       61 gatgtagtaa gggccccaac tgtcaacgat ggccgtgagc tgcgccttgg gtgcgaaggc
      121 catcatgtcc gaggcctggc caaagccgaa ttttccctgt tcctgtgcga tctgcagggg
      181 cgcggtggag tccgtgtgct ggacaagaat gtctgcgccc tggctcaata gaaccttggc
      241 ggcatcggct tcctttcccg gatcaaacca ggtgttggcc cagatgatct tcaccttgaa
      301 gtccggattg acggtctgcg cccccagcat aaaggcattg atgccggcga tgacttcggg
      361 gatcgggaag gacacgatat agcccgccgt tccggtcttt gacatcttgg cggcgatctg
      421 gccgatcaca taacggccct catggaattt ggaattgtag gttgtgacgt tgggcgctgt
      481 cttgtagccc gtcgcatgct cgaatttcac atcggggaac ttcgccgcaa ccttcagggt
      541 gggctccatg aagccaaatg aagtcgtgaa aattatcttg cagccgtcgc gtgccagacg
      601 ctcgatggtg cggtcggaat ccggttccgc cacgttctca acgaacacgg tttcaacctt
      661 gtcgcccagt tcggccacca ccgccttgcg gccctcgtcg tgctggaacg accagccgcc
      721 gtcgcccacc gggcccacat aaatccagca ggccttgtag gcctcagcca gcgaggccgt
      781 tgatcccagc cccagcgcca ccgccgccac cgcggctgtc atcagtaatt tcttcatggt
      841 ctatctccct gtcattcggt tcagtctgcg gctttgttcg atgtgacccc gtccgccgcg
      901 ctggcagggc cggttgctaa ttatctatca ggcacaaaag attgggcaag gcaagccggt
      961 gtattggcct gcgtcagccg cttgtttccg gaaatgatca ccagcgcgat gatcgtcacg
     1021 atatagggca gcgacgaaag gaattgcgac gggatgccaa gccccatgga ctggacgaca
     1081 aagcccagaa tggagacagc gccgaaaaga taggcgccca ccagcgtgcg caggggccgc
     1141 caggaggcaa aaacgacaag ggcaagtgct atccagcccc gtcccgccgt catgttctcg
     1201 atccattgcg gcgtataggc aagcgacagg tagcccccgg caaggccggc gcaggccccg
     1261 ccaaacatca cactgagata gcggatggcg atgacccggt aacccagggc atgggccgag
     1321 ctatggctgt tgccgaccgc gcggatcacc agcccggtct tggttctgaa cagcacatag
     1381 gccacgcccg ccgtcagcag gagggaaaac ggcacaagcg cgtcgatgcc gaaaagcgtt
     1441 ggcatcttga cgcccggctg gccgacaaaa gcctcgccga tcaggccgga aaggccaagc
     1501 cccaggagcg tgagcgcaag ccccgccgcc acctggttgg ccaccagcgt cagcgtcagg
     1561 aatccaaaaa gggccgacat ggcgataccg gcgaagatgg cggcccccac tcccagcagg
     1621 gttgagcctg tcatgaaggc caccgcaaac cccgccacgg cccccatcac catcatgcct
     1681 tcgacaccca ggttgagcac cccggaacgt tcgaccacca gttcaccaat ggcggcaatc
     1741 agcagcggcg ttgcggcggt aatgatggtg agcatcactg cgaggctttg gtccatcgcc
     1801 ctactccgcc gccttgagcg cgcccacacg gcccatctcg agtttgatcc ggtagagaat
     1861 gagcgtgtcg caggacagga tgaagaacaa gaggatgccc tggaatacct tggcgatctt
     1921 gtcggaaatt cccaatgtca cctgcgccgc ctcgccgccg atatagctca gcgccagcag
     1981 caatccggca aagagcacgc cgagcggatt aaggcggc
//
LOCUS       DAORVN010000432         2015 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_60295_length_2015_cov_2.805612, whole genome shotgun sequence.
ACCESSION   DAORVN010000432 DAORVN010000000
VERSION     DAORVN010000432.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2015
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60295_length_2015_cov_2.805612"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..635)
                     /locus_tag="PKV23_08625"
     CDS             complement(<1..635)
                     /locus_tag="PKV23_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018183850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85538.1"
                     /translation="MQKSNAMAVVVPVALTLMLAPSALAQDMKMGFFVTSVGSGKGAD
                     LGGLDGADKHCQTLAEAAGSTGQTWRAYLSTQGDGAVNARDRIGAGPWANAKGEVIAK
                     DVAELHGANNLTKATALTEKGEVVNGRGDTPNMHDILTGSQADGTAFAAGEDRTCGNW
                     TLSGTEGAVMVGHSDRTGLDDSAPAKSWNTSHPSRGGCSQDALKGTGGNGL"
     gene            775..>2015
                     /gene="sbmA"
                     /locus_tag="PKV23_08630"
     CDS             775..>2015
                     /gene="sbmA"
                     /locus_tag="PKV23_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012061443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HQX85539.1"
                     /translation="MFVSFFPTPKLFFGSTVLWTLAAILLWYFAGDSMGTALGLLPAP
                     DDPALKASVFVFISRPFIWFYIYFTVAVALFAGFWEWYAPHRWSRWSILGSALIIFTT
                     YFQVQVSVAINAWYGPFYNMIQQALAAPGQVTLAQFYGQLATFAGIAMIAVFVGVLTR
                     FFVSHYIFRWRTAMNDYYMANWPKLRFVEGASQRVQEDTMRFSTTMEGLGVNLIDSVM
                     TLIAFLPVLTALSVNVTELPLIGHIPNALVVIAIVWSLFGTAFLALIGIRLPGLEFRN
                     QRVEAAYRKELVYAEDDERRAQPQTVAELFAAVRANYFRLYFNYMYFNVGRIFYLQTD
                     NIFPYIVLAPTIVAGKITLGAMNQILNAFAQVRSSFQYLVNSWTVIVELLSIYKRLRA
                     FEATLRGEELPAIERIPDDSGV"
BASE COUNT          321 a          611 c          619 g          464 t
ORIGIN      
        1 aaaaggccat tgccgccggt gcccttgagg gcgtcctggc tgcagccgcc gcgcgagggg
       61 tgggaggtgt tccatgactt ggcgggcgct gagtcatcaa ggccggtgcg gtcgctatgg
      121 cccaccatca cggcgccctc cgtcccgctc agggtccagt tgccacaggt gcggtcctcg
      181 cctgcggcga aggccgtgcc gtcggcctgg ctgccggtga ggatgtcgtg catgttgggc
      241 gtgtcgccgc ggccattcac cacctcacct ttttcggtca gggcagtggc cttggtgagg
      301 ttgttggcgc catgaagctc cgccacatcc ttggcgatca cttcgccctt ggcattggcc
      361 caggggccgg caccgatgcg gtccctggcg ttgaccgcgc catctccttg agtggaaaga
      421 taggcgcgcc aggtttggcc ggtggaaccc gccgcctcgg caagggtctg gcaatgtttg
      481 tcggcgccat caaggccgcc caaatcagcg cccttgccgg agcccacgct ggtgacgaag
      541 aatcccattt tcatgtcctg ggcgagcgct gaaggcgcca gcataagggt gagggcaacg
      601 ggcacgacga cggccatggc atttgatttt tgcatttttt tctccttttg ttttccgccc
      661 ggatgaagcc tagtgggcgg cccatggcgg tacaattcaa ctttacttga cctagcccgg
      721 ttgccgggat tgcgctaacc ctgctccggt acccagcgca gaaaggccag ctccatgttc
      781 gtttccttct tcccgacgcc caaactgttc ttcgggtcca cggtgttatg gacactcgcc
      841 gcgatcctgc tctggtactt tgcgggcgac agcatgggga cagcgcttgg cctgctgccg
      901 gcgccggatg atcccgctct taaggcaagc gtgttcgttt tcatctcgcg tcccttcatc
      961 tggttttaca tttatttcac ggtggcggtg gcgctctttg ccgggttctg ggagtggtac
     1021 gcgccgcacc gctggtcgcg ctggtcgatc ctcggttcgg cgctcatcat cttcaccacg
     1081 tatttccagg tgcaggtgag cgttgccatc aatgcctggt atggcccgtt ctataacatg
     1141 atccagcagg cgctggcggc gccggggcag gtcacgctgg cgcagttcta tggccagctt
     1201 gccacctttg cgggaattgc catgatcgcg gtgttcgtcg gcgtgctcac ccggtttttc
     1261 gtcagccact acatcttccg ctggcgcacc gcgatgaatg attactacat ggccaactgg
     1321 cccaagctgc ggtttgtcga aggcgcctcg cagcgcgttc aggaagacac catgcggttt
     1381 tcaacaacca tggaggggct gggggtcaac ctgattgatt cggtgatgac gctcattgcc
     1441 tttctcccgg tgcttacggc gctctcggtc aatgtcacgg aacttccgct gatcggccat
     1501 atcccgaatg ctctcgttgt cattgccatc gtctggtcgc tcttcggcac ggcctttctg
     1561 gcgctcatcg gcatcaggct gccgggactg gagtttcgca accagcgcgt cgaggcggct
     1621 taccgcaagg aactggtcta tgcggaagat gacgagaggc gggcccagcc gcagacggtg
     1681 gcggagctct tcgcggcggt gcgcgcgaat tacttccggc tctatttcaa ctacatgtat
     1741 ttcaatgtcg ggcggatatt ctacttgcag accgacaaca tctttcccta catcgtcctg
     1801 gcgcccacaa tcgtggcggg aaagatcacc ctgggggcaa tgaaccagat cctcaacgcc
     1861 ttcgcccagg tgcgctcatc gtttcagtac ctggtcaatt cctggacggt gattgtcgag
     1921 ctcctgtcga tctacaagcg gctgcgggcg tttgaagcga cgctgcgcgg cgaggaactg
     1981 ccggcaatcg aaaggatccc ggacgattcg ggggt
//
LOCUS       DAORVN010000433         2010 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_60590_length_2010_cov_2.572379, whole genome shotgun sequence.
ACCESSION   DAORVN010000433 DAORVN010000000
VERSION     DAORVN010000433.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60590_length_2010_cov_2.572379"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..713)
                     /locus_tag="PKV23_08635"
     CDS             complement(<1..713)
                     /locus_tag="PKV23_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008874916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HQX85540.1"
                     /translation="MKRGADLIMTGGTLVNHDGVAPAGLAIAEGRIVGIGDVSSFAAA
                     ETLDCRGLHILPGVIDSQVHLREPGLEHKEDLESGGRAAVMGGVTSVFEMPNTKPLTV
                     TAAALADKVRRASNRMYCDFAFYMGGTRENVADLAAFEKLPGCAGIKVFMGSSTGELL
                     VEDDEGVASILAQTSRRAAFHSEDEFRLRQRVNEQVAGNPASHPVWRDAEAARLCTER
                     LVRIARRTGKRIHVLHVSTA"
     gene            779..1591
                     /locus_tag="PKV23_08640"
     CDS             779..1591
                     /locus_tag="PKV23_08640"
                     /inference="COORDINATES: protein motif:HMM:TIGR03317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein"
                     /protein_id="HQX85541.1"
                     /translation="MQVRRERTVLGLEEAETENFLHNLVTVDILGLAQGAARYAALLT
                     PQGKILFDFFVVKTAEGYLLDCAASQLEELTKRLVFYRLRAKITISERKDLEVGVSPE
                     RPQGMTAYADPRTSLIGWRVIADKGTLPMGTGYDAARIALGLGDSDGDIGSSELFPHE
                     ANFDQMGAVSFSKGCYIGQEVVSRMEHRATARSRILPVTLDGAAPPRGAAIKSGDRII
                     GSVLSSAGNTALALLRLDRLAETTQPLLTEAVRVHVHKPAWIKYDVPSKDYA"
     gene            1591..>2010
                     /locus_tag="PKV23_08645"
     CDS             1591..>2010
                     /locus_tag="PKV23_08645"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HQX85542.1"
                     /translation="MSPVVHEDGICRCTWCGTDPVYVAYHDTEWGVPEYDSRALFEKL
                     QLDGFQAGLSWITILRKREAFRKAFDDFNPEKMARYRPAKLERLMNDAGIVRNRLKIE
                     ASVSNAQAYLAIPDFSAYLWNFMDGKPLQNRLRSMKDV"
BASE COUNT          406 a          562 c          635 g          407 t
ORIGIN      
        1 gcggttgaga catgcagaac atggatgcgt tttccggtgc ggcgggcaat gcggacgagg
       61 cgttcagtgc aaagccttgc cgcttcggca tcgcgccaga cgggatggct cgcgggattg
      121 ccggccactt gttcgtttac acgctggcgc aggcggaatt catcctcgct gtggaaggcg
      181 gcgcggcggc tagtctgcgc cagaatgctt gccaccccct catcatcctc gacgagcagt
      241 tcaccggtgg acgagcccat gaacaccttg atgccggcgc agcccggcag cttctcgaag
      301 gcggcgagat cggccacatt ttccctagtg ccacccatgt aaaaagcgaa atcgcaatac
      361 atgcggttgc tggcgcggcg gaccttgtcg gccagggctg cggcggtcac ggtaagcggc
      421 ttggtattgg gcatttcgaa aacgctggtg acgccgccca tgaccgcggc gcggccgccg
      481 gattccaggt cttccttgtg ttcaaggccg ggctcgcgga gatgcacctg gctgtcgatg
      541 acgccgggga gaatgtgcag gccccggcaa tccagggttt cggcggcggc gaaagaactg
      601 acatcgccta tccccacgat acggccttcg gcaatggcca gaccggcggg cgccacccca
      661 tcatgattga ccagggttcc ccccgtcata atgagatcag ccccgcgttt catccagatt
      721 ccttgattgt ggcacaaagc aaccgtaaat ggtgctgatg gcgaattcta gctttttaat
      781 gcaggtgcgg cgtgagcgca cggtcctcgg ccttgaagag gctgagaccg agaatttcct
      841 gcataacctg gtgacggtgg atatcctggg gctggctcaa ggcgcggcgc ggtatgccgc
      901 cctgctcacg ccccagggca agatcctgtt cgacttcttc gtggtgaaaa cggcggaagg
      961 atatttgctc gactgcgcgg cttcccaact tgaagagctt acgaagcgcc tggtgtttta
     1021 ccggctgcgg gcgaagatta cgatttccga gcgcaaggac ctggaagttg gggtttcacc
     1081 ggagcggccc caagggatga cggcctatgc cgatccgcgc acatcgctaa ttgggtggcg
     1141 ggtgattgcc gacaaaggca cgttgccaat gggcaccggc tatgacgctg cgcgcattgc
     1201 tttggggctg ggcgacagcg atggcgatat cggttccagc gagttgtttc ctcatgaagc
     1261 gaatttcgat cagatgggag cagtgagttt cagcaagggc tgctacatcg gccaggaagt
     1321 ggtctcgcgc atggagcacc gcgccacggc gcggagccgt attcttccgg tgacgcttga
     1381 tggggcagca cctccacgcg gcgcggcgat caaaagcggt gacaggatta ttggctcggt
     1441 tctgtcatcg gcgggaaaca cagcgctggc tctgcttcgc ctggatcggc tggctgaaac
     1501 aactcagcca ctgctgacag aggctgtcag ggtacatgtg cataagccgg catggataaa
     1561 atatgacgtg ccatcaaagg attacgccta gtgtctccag tcgttcatga agacgggatt
     1621 tgccgctgca cgtggtgcgg cacggatccg gtttatgttg cctaccacga cacggaatgg
     1681 ggcgtgccgg aatatgattc acgcgcgctc ttcgaaaaac tgcagctcga cggctttcag
     1741 gcgggcctct catggatcac gatcctgcgc aagcgcgaag cattccgcaa agccttcgat
     1801 gatttcaatc cggaaaaaat ggcgcgctac aggccggcaa agcttgagcg gctgatgaat
     1861 gacgcaggca tcgtgcgcaa ccggctgaag atcgaagcca gcgtgagcaa cgcccaggcc
     1921 tatctggcca ttccggattt ttccgcttac ctgtggaatt tcatggatgg caaaccctta
     1981 cagaacaggc tgcgcagcat gaaggatgtt
//
LOCUS       DAORVN010000434         2008 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_60691_length_2008_cov_3.290835, whole genome shotgun sequence.
ACCESSION   DAORVN010000434 DAORVN010000000
VERSION     DAORVN010000434.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2008
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60691_length_2008_cov_3.290835"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..704)
                     /locus_tag="PKV23_08650"
     CDS             complement(<1..704)
                     /locus_tag="PKV23_08650"
                     /inference="COORDINATES: protein motif:HMM:NF012981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HQX85543.1"
                     /translation="MKRAGLLINPSSGKSSGKGLSLVDMLKHNTAVSTRVLERFEQLP
                     GFLDDFASDGVTDLFISSGDGTIHAVQTDLAERKPFKVIPRLSLLPHGTTNMTAADLG
                     FPHRGLRAQADYITHLEAKQIRERPTLRVVNPRDGRPRHGMFLGTGAASEATLFCQRV
                     FNAKGVKGNWATFATLASAAGRTMFTRPNPHDVTRFDRPFPIRVTSDGREFSDGPQLL
                     AFSTTLEKLILGARPF"
     gene            complement(701..>2008)
                     /locus_tag="PKV23_08655"
     CDS             complement(701..>2008)
                     /locus_tag="PKV23_08655"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HQX85544.1"
                     /translation="LVPWPQPSAFASTGFSMTTKPRAKPAKKSPAKKLRKEPVPETVS
                     IPKAEVPRAPIHFSPVLCILNAPQQKLWSLSLAERLKKQFAAAGLAMTVSEAEARTHN
                     GPVILVRGDAVIDQPLIPVLLKRPNFLLLTDESASPSPIAASVRGSDVSRAIEILLGT
                     KSLADAKLLARAPAQLDMEFWKSLRKRETPYAMVVTAENRSAVEWRMFMGTYKGATDF
                     ITKHLWPVPAFHATRFLAPRGVSPNMVTTIAALMTVLAFWFFLQGQFIPGLIAAWAMT
                     FLDTVDGKLARTTLTSSKWGDYFDHGIDLIHPPFWYIAWGYGLLATGTQWSSEVFWGV
                     MAAILGGYVLQRAIEGIAIKWLGLEIHIWRPIDTFFRQITARRNPNLVVLTFFTAIAR
                     PDWGLLAVAAWTVICLGLHALQLLMAFAEKRRNGPLSSWMTKP"
BASE COUNT          393 a          550 c          678 g          387 t
ORIGIN      
        1 cagaaaggcc ttgccccgag gatcagtttt tccagcgtgg tggagaaggc cagaagctgg
       61 ggcccatcgc tgaactcccg gccatcgctg gtgacgcgaa tgggaaaggg gcggtcaaaa
      121 cgggtgacat catgggggtt gggccgggtg aacatggtgc ggccggcggc gctggccagg
      181 gtcgcaaagg tggcccagtt gcccttgacc cccttggcgt tgaaaacccg ctggcaaaac
      241 agggtggctt cggaggccgc gccggttccc agaaacatgc cgtgccgcgg cctgccatcg
      301 cgggggttga cgacccgaag ggtagggcgt tcacggatct gttttgcttc caggtgggtg
      361 atgtaatcgg cctgggcccg cagcccccgg tggggaaagc ccagatcggc ggccgtcatg
      421 ttggtggtgc catggggcag gaggctgagg cgcggaatga ccttgaaggg cttgcgctcc
      481 gccaggtcgg tttgcacggc atggatggtg ccatcgccgg aactgatgaa cagatcggtg
      541 acgccgtcgc tggcaaagtc atcgagaaag cccggcaact gctcaaacct ttcaagcacg
      601 cgggttgaaa ccgctgtgtt gtgtttcagc atgtcgacaa gactcaagcc cttgccgctg
      661 gattttcccg agcttggatt gatcaggagg ccggcgcgct tcacggtttc gtcatccagg
      721 acgaaagcgg cccgttgcgg cgcttttcgg cgaaggccat gagcaactgc agcgcatgaa
      781 ggccgaggca aattaccgtc caggcggcaa cagccagcag gccccagtca ggccttgcga
      841 tggccgtgaa gaatgtgaga acgaccagat taggatttcg ccgtgcagtg atctggcgga
      901 agaaggtatc gatcgggcgc cagatgtgaa tctctaggcc cagccatttg atggcaatgc
      961 cctcaatcgc gcgctgcaga acgtagccac cgagaatggc cgccatcacg ccccagaaga
     1021 cttcgctgct ccattgggtt ccggtggcca gcaggccata gccccaggca atgtaccaaa
     1081 agggcggatg gatgagatcg atgccgtgat cgaaatagtc gccccatttg cttgaggtca
     1141 acgtggtgcg cgccagcttg ccatccacgg tgtcgagaaa cgtcatggcc caggcggcga
     1201 tgagtcctgg aatgaactgg ccttgcagga aaaaccagaa tgccagcaca gtcatgaggg
     1261 cggcaattgt ggtgaccatg ttgggggaga caccgcgcgg tgcgagaaac cgggtggcat
     1321 ggaatgccgg cacgggccac aggtgcttgg tgatgaaatc ggtggcgccc ttgtaagttc
     1381 ccatgaacat gcgccactcg acggcgctgc ggttttcggc ggtgaccacc atggcatagg
     1441 gcgtttcacg tttgcgcagg gatttccaga actccatgtc gagctgcgcc ggggcgcggg
     1501 ccagcagttt tgcatcagca agggatttgg ttcccagcag gatttcgatg gcgcgggaaa
     1561 cgtcgctgcc gcgcacgctg gcggctatgg gcgaagggct tgctgattca tccgtgagga
     1621 gcagaaaatt ggggcgcttg agcaggacgg ggatgagggg ctggtcgatc accgcatcgc
     1681 cgcgcaccag aatgaccggg ccattgtgcg tgcgtgcttc cgcctcactg acggtcatgg
     1741 caaggcccgc cgcggcgaac tgctttttca gccgttccgc cagggacagt gaccacaatt
     1801 tctgttgcgg ggcattcaag atgcagagca ccgggctgaa atgaataggc gcccttggca
     1861 cttcggcttt cggaatgctg accgtttccg ggacaggctc cttccgcagc tttttcgctg
     1921 gtgatttctt tgcgggcttg gcccttggct tcgtcgtcat gctaaaccct gtcgatgcga
     1981 aggctgaggg ctgtggccag ggcaccaa
//
LOCUS       DAORVN010000435         2008 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_60700_length_2008_cov_2.896057, whole genome shotgun sequence.
ACCESSION   DAORVN010000435 DAORVN010000000
VERSION     DAORVN010000435.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2008
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60700_length_2008_cov_2.896057"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..501
                     /locus_tag="PKV23_08660"
     CDS             <1..501
                     /locus_tag="PKV23_08660"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016735076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HQX85545.1"
                     /translation="ELDGIRSAARMAVHAHNTLHPGERGSMAPELRALFAEVADDVFI
                     EAPFHCSYGINTRLGSRVYLNAGCTILDSGRVCIGADTMLGPGVHIYCAEHHKDPVLR
                     AKGLEIALPVEIGKNVWVGGGAIILGGVTIGNGALVGAGAVVTKDVPPGATVVGNPAR
                     QIGGGA"
     gene            565..921
                     /locus_tag="PKV23_08665"
     CDS             565..921
                     /locus_tag="PKV23_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HQX85546.1"
                     /translation="MTYVDGFVLPVPKKNLAAYRKMAAKASKVWMKHGALSYVEAVAD
                     DVKPGKSTSFPQSVKLKDGETVIFAWITYKSRKHRDQVNAKVMKDPYMANFDPKTMPF
                     DGKRMFWGGFKKLVAM"
     gene            1015..1758
                     /locus_tag="PKV23_08670"
     CDS             1015..1758
                     /locus_tag="PKV23_08670"
                     /inference="COORDINATES: protein motif:HMM:NF018473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1223 domain-containing protein"
                     /protein_id="HQX85547.1"
                     /translation="MTCLLTRRNALALGAAAVLARPALAAVKRPVIVELFTSQGCSSC
                     PPADAYFKALKDQPDVVALSYHVDYWDYLGWRDTLGSPECSQRQYDYAKSRGDKNVYT
                     PQTIINGGGHFVGSQRARVSSGIDVARSETETDWVDMEMTHNNTDISISIPAGKPISE
                     ATLWLMAFTPHVSTEIKKGENAGHTVDYFNVVRKMVPAGMWHGEAAKIVLPKGSVVPE
                     SCKGWVALLQEGTVGRVIGAVTGGASPNA"
     gene            complement(1759..>2008)
                     /locus_tag="PKV23_08675"
     CDS             complement(1759..>2008)
                     /locus_tag="PKV23_08675"
                     /inference="COORDINATES: protein motif:HMM:NF013989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HQX85548.1"
                     /translation="EEALIQAQAAAAGKVVIVMGASVAQQMLKQRLADEIYIQLVPVL
                     LGGGSRLFENFGAAPLELVCDRAVNSPYITHLKYKIPK"
BASE COUNT          432 a          557 c          634 g          385 t
ORIGIN      
        1 gaacttgatg gaatacgcag tgctgcccgc atggccgtcc atgcgcacaa caccctgcac
       61 ccgggtgagc gcggcagcat ggcgccagag cttcgcgcgc tttttgccga ggttgcagac
      121 gacgtgttca tcgaagcgcc gttccattgt tcctatggca tcaacaccag gctgggaagc
      181 cgcgtctatc tcaatgccgg ctgcaccatc ctagattccg gcagggtgtg catcggggcg
      241 gataccatgc tcgggccggg cgttcatatc tactgtgccg aacaccacaa ggatccggtc
      301 ctgagggcga aggggctcga gattgcgctg ccggtggaga tcggcaagaa cgtgtgggtc
      361 ggcggtggag ccatcatcct gggcggggtg acgatcggca acggggcgct cgtgggcgcc
      421 ggcgcggtag tgacgaaaga cgtgccgccg ggcgcgacgg tggtgggcaa tccggcgcgg
      481 cagatcggcg gcggtgcctg acccgtgcgg aacaattgat ccggcaatcc agttatgaag
      541 tttgcaacaa gaaggaattt gacgatgacc tatgttgacg gatttgttct tcccgtgccg
      601 aagaaaaacc tggcggccta ccgcaagatg gcggcgaagg cgagcaaggt atggatgaaa
      661 catggcgcgc tttcctatgt ggaagcagtt gcagatgatg tgaagccggg aaaatccact
      721 tcgttcccgc agagcgttaa gctcaaggac ggggaaacgg tgatatttgc atggatcaca
      781 tacaagtccc gcaagcaccg ggaccaggtg aatgccaagg tcatgaagga cccctacatg
      841 gccaatttcg acccaaagac aatgcctttt gacggaaagc gcatgttctg gggcggcttt
      901 aagaaactgg tagccatgta ggccggattg ccgcggaaac ctgcaacaaa catgtgatca
      961 tgcctcaccg gctcgtgagt agctgaatgg tgcattagtt gctatatccg ccccatgacg
     1021 tgtttactca cccgccggaa tgcccttgcc cttggcgctg ccgccgtgct tgcgagaccg
     1081 gcacttgcgg cggtcaagcg cccggttatc gtggagctgt tcaccagcca gggctgctcg
     1141 tcctgcccgc cggcggatgc ctatttcaag gccttgaaag accagcccga cgtcgtggcc
     1201 ctgtcctacc atgtggatta ctgggattac ctgggctggc gcgacacact tggcagcccg
     1261 gaatgttcac agcggcaata tgactatgcc aaatcgcgcg gcgacaaaaa cgtctatacg
     1321 ccgcaaacca tcatcaatgg cggcgggcat tttgtcggaa gccagagggc ccgtgtttcg
     1381 agcggcatag acgtggcaag aagcgaaacc gaaaccgact gggtcgacat ggaaatgacc
     1441 cacaacaata ctgatatctc gatcagcatt ccggccggca agcccataag tgaagccacc
     1501 ctttggctca tggcttttac cccccatgtt tccacggaaa tcaagaaggg cgagaatgcc
     1561 ggacacacgg tcgactactt caatgtggtg cgcaagatgg tgccggccgg catgtggcat
     1621 ggcgaggcgg cgaagatcgt gctgcccaag ggaagtgtgg ttcccgaaag ctgcaaaggc
     1681 tgggtggcgc tgctgcagga aggcactgtg gggcgggtca tcggcgcggt caccggcggg
     1741 gcatcgccca acgcctgatc atttcggaat cttgtatttc aggtgggtga tgtaggggga
     1801 gttgacggcc ctgtcacaga cgagttcaag cggagcggcg ccgaaatttt caaacaggcg
     1861 gctgccgccg cccagcagaa ccggaaccag ctgaatgtag atttcatcgg cgagcctttg
     1921 cttcagcatc tgctgggcga cactggcgcc catcacgatc acaacctttc cggcagcggc
     1981 ggcctgggcc tggatcaggg cctcttcg
//
LOCUS       DAORVN010000436         2008 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_60703_length_2008_cov_2.708141, whole genome shotgun sequence.
ACCESSION   DAORVN010000436 DAORVN010000000
VERSION     DAORVN010000436.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2008
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60703_length_2008_cov_2.708141"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..365)
                     /locus_tag="PKV23_08680"
     CDS             complement(<1..365)
                     /locus_tag="PKV23_08680"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQX85549.1"
                     /translation="MASHLPKPSVYVVDDDDAVRDSLDLYLTLKGMNVTIFGSAQELL
                     DHDAGAPHILVLDVNMPGMDGIMLLEILRGRGHAEPAVLITGLGDPEIRARAERAGAT
                     AFLDKPIDPPVLFSALTRLL"
     gene            complement(483..1121)
                     /locus_tag="PKV23_08685"
     CDS             complement(483..1121)
                     /locus_tag="PKV23_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012173174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQX85550.1"
                     /translation="MQASEVVHVIDDDEGVREGLCALLDAKGHAARSHASCEEFLSGF
                     SADSTLCIVADLRMPGMGGLDLQSHLLKNAIDLPFIVITGHGDVSSAVRALKSGAADF
                     IEKPIDGKAFIAAVERAVVARRANRAQSEATHLAHERVAALTPRERDVLRHLVAGSPN
                     KIIAHQLRISPRTVENHRARLMVKMQADSVAELVRMALAAGISAQNEGQTGE"
     gene            complement(1108..>2008)
                     /locus_tag="PKV23_08690"
     CDS             complement(1108..>2008)
                     /locus_tag="PKV23_08690"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQX85551.1"
                     /translation="IIGIGRVVTGQRKDGSTFPLELSIGEARIGDERLFAGFIRDLTD
                     RQQMEQRVHELQEELVHASRLASLGEITSMVAHEVNQPLSASGTYLEVARELLVSERE
                     DRLAGGLKAIEQAAAQIRRVGETVRRIREFARKKTPDLAFEDINRIIEEANAIAAVGT
                     KAKNIRTMFDLSALHPRTRVDRIQIQQVVMNLVRNAMDAMTDHERRELVLQSRINEAG
                     EIEISVLDSGPGISEAAAKRLFTPFMTTKKDGTGLGLAICRSIVEAHGGRLWHEKSPL
                     GGAAFKFTLPANTGSDVTPHASI"
BASE COUNT          363 a          644 c          607 g          394 t
ORIGIN      
        1 agcagccggg tgagggcgga gaacagcacc gggggatcaa tcggcttgtc gagaaatgcc
       61 gtcgcgccgg cccgctcggc cctggctctg atctcgggat cgcccagccc ggtgatcagg
      121 acggcgggct cggcgtggcc gcgcccccgc aggatttcaa gcagcatgat cccgtccatg
      181 cccggcatgt tgacgtcaag caccaggata tgcggcgcgc ccgcgtcatg gtcgagcagt
      241 tcctgggccg agccaaaaat cgtgacgttc atgcccttca gcgtcaggta gaggtcaagc
      301 gaatccctga cggcatcatc atcgtcgacg acatagactg atggctttgg aagatgtgat
      361 gccaaggtag ggccctagat cagcttgttt ccggacggcg aaatccacga cttcgcccaa
      421 tggccggtca tactgggcca gcgcaggggg cagcgcctag tccggaaaaa cacctaggca
      481 cgctattcgc cggtttggcc ttcattttgg gccgaaatcc ccgccgccag ggccatgcgc
      541 accagttcgg ccacgctgtc ggcctgcatc ttaaccatga gccgcgcccg gtggttttcg
      601 acggtgcgcg ggctgattct gagctgatgg gcgatgatct tgttggggct gccggcaacc
      661 agatggcgga gcacatcgcg ttcgcgcggc gtcaatgccg ccaccctttc atgggcgaga
      721 tgggtggctt ccgactgggc cctgtttgcc cgccgcgcca caaccgcccg ttcgactgcg
      781 gcgatgaagg ctttcccgtc gatcggcttc tcgatgaaat cggccgcacc cgacttcagc
      841 gcccgcaccg cgctcgacac atcgccatgc ccggtgatca cgatgaacgg caaatcgatt
      901 gcgttcttca gcaggtggct ttgcaggtca aggccgccca tgccgggcat gcgcaggtca
      961 gccacgatgc agagggttga atcggcggaa aagccgctca ggaattcttc gcatgaggca
     1021 tgggaccggg ccgcatggcc tttcgcatcc agcagtgcgc atagtccttc gcgcaccccc
     1081 tcgtcgtcat cgatgacatg gacaacttca gatgcttgca tggggcgtca cgtcacttcc
     1141 cgtattggcc ggcaatgtaa acttgaacgc tgctccgccc aaggggcttt tttcatgcca
     1201 gagccggccg ccatgggcct cgacgatgga ccggcaaatg gcgaggccaa gccccgtgcc
     1261 atccttcttt gtcgtcatga agggcgtgaa aagacgcttt gcggcggctt ccgaaatgcc
     1321 ggggccggaa tccaggacac tgatttcaat ctcgcccgcc tcgttgatcc gagattgcag
     1381 gacaagctcc cgccgctcat ggtcagtcat cgcgtccatc gcattgcgca cgagattcat
     1441 gacaacctgc tggatctgga tgcggtccac ccgggttcgc ggatgcaggg cggaaaggtc
     1501 gaacatcgtc ctgatgtttt tcgccttggt gcccaccgcg gcaatcgcgt tagcttcctc
     1561 gatgatgcgg ttgatgtctt caaacgccag atcgggcgtt ttcttccggg caaattcgcg
     1621 gatgcgccgg accgtttcac caacacgccg gatctgcgcc gccgcctgct cgatggcctt
     1681 caggccgccc gccagccggt cttcacgctc cgacaccagg agttcacggg caacttccag
     1741 ataggtgccc gacgccgaca ggggctggtt gacctcgtgg gcgaccatcg acgtaatctc
     1801 gccaaggctt gccagccgcg aggcgtggac cagttcctcc tgcagttcat gcacccgctg
     1861 ctccatctgc tggcggtccg tgaggtccct gatgaatccc gcaaaaagcc gctcgtcgcc
     1921 aattctcgcc tcgccgatcg aaagctccag gggaaaagtg gagccatcct ttctctggcc
     1981 cgtgaccacc cggccgatgc cgatgatg
//
LOCUS       DAORVN010000437         2003 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_61019_length_2003_cov_2.239733, whole genome shotgun sequence.
ACCESSION   DAORVN010000437 DAORVN010000000
VERSION     DAORVN010000437.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61019_length_2003_cov_2.239733"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2003)
                     /locus_tag="PKV23_08695"
     CDS             complement(<1..>2003)
                     /locus_tag="PKV23_08695"
                     /inference="COORDINATES: protein motif:HMM:NF024516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3971 domain-containing protein"
                     /protein_id="HQX85552.1"
                     /translation="AKVDVPLSGHAEIEISDAGVITSASAEFAAAAGVVGLPGYIAQP
                     IVIDEGALRVDYDAPTGGFKIIDSIILVGGSRAELTGRLDPVRARDGRLTDLKIDLKA
                     TNVSVDTQGTVKYPVLVDRIEFLGKASVEGAVMDIEDLVVMSGNAGVRLRGTITGGDE
                     SAGIRLSGRVRDLSADFLKKLWPPIVVPKSRNWVTENVQTGRITEGAFNVDIPVNGLA
                     AALRQKLLPNETIDFRFSMADVTSTYFKSLPPLLGASGHARLQGDSFELTVESGKVIL
                     PSKGTVQVSQTTFKAEKLLTNPVPAAFSLNLSASAPNLMELARLPDLNLLRNANFVPP
                     DIGGQAEAKINFSMPLMKDLPREQIVTTAEIKVADASIKQVIPNVDLSDGSLLVAFDR
                     SGITASGPVRINGFPSKVSWARPAGPGTRATASIETELDDKGREKLGITLGDYLRGPV
                     QIMADIDGVGEKNVCMKVKANLAKDEMFIDAISWRRPPTTNTTATFTYRSGDAAGRKV
                     ENLVVKGQSLSLKGDVILTSTGGLKSAKFSQVWLSDENNFIMTMVPAGEGQAIDISGQ
                     SFDARPFIKSIFARDSGKPDRSAPRQNLTVKAAFDRVIANRGEILTGVSANISVRNSL
                     IASADIEGKFLSERALAIRVRPNVTGRQLQITGHGEHAGRAQW"
BASE COUNT          406 a          585 c          580 g          432 t
ORIGIN      
        1 ccactgagct cggccggcgt gctcgccgtg accggtaatc tgcagctggc ggccggtgac
       61 attgggacgc acacggatcg ccagggctct ttcagacagg aatttgccct cgatatcggc
      121 actggcgatc agactgttgc gcaccgagat gtttgccgat accccggtga gtatctctcc
      181 gcgattggca atgacccggt caaaggccgc cttgacggtc aggttctggc gcggcgccga
      241 cctgtcgggc tttccggagt ccctggcgaa tatggatttt atgaatggac gggcatcaaa
      301 cgactgtccg ctgatgtcaa ttgcctggcc ttcgccggcg ggcaccattg tcatgatgaa
      361 gttgttttcg tcgctgagcc agacctgtga aaactttgcc gatttcaagc cgccggttga
      421 cgtcagtatg acgtcgccct tcaggctgag gctttgtccc ttgacgacga gattctcaac
      481 tttccgcccg gccgcatcgc ccgaacgata ggtgaaggtc gctgtggtgt ttgtggttgg
      541 cggcctgcgc cagctaattg catcaataaa catctcgtcc tttgccaggt tggccttcac
      601 tttcatgcac acgttctttt cgccaacgcc gtcaatgtcc gccatgatct gaaccggtcc
      661 gcgcaaatag tcgccgagcg tgataccgag tttttcgcgc cccttgtcat caagttccgt
      721 ttcaatggaa gcggttgccc tggtgcccgg accggcgggc ctggcccatg acaccttgga
      781 ggggaagccg ttgatcctga ccggcccgct tgcagtaatg ccgctccggt caaacgccac
      841 aaggagcgag ccatcggaca aatcaacatt gggaatgacc tgcttgatgc tggcatcggc
      901 cacttttatc tcggcggtgg tcactatctg ctcacgcggc aaatccttca tcaggggcat
      961 tgaaaaattg atcttggcct cggcctgccc accgatgtcg ggcggaacaa aattcgcatt
     1021 ccgcaagaga ttgagatcag gcagcctcgc cagttccatc aggttgggcg cactggcaga
     1081 aagattgagg gagaacgcag ctggcacggg attagtcaaa agcttttcgg ccttgaaggt
     1141 cgtctggctc acctgcacgg tacctttcga cggtaaaatc actttgccgg attcgaccgt
     1201 cagttcaaaa ctgtcaccct gcaagcgggc atgcccagaa gcacctagca gcggcggcag
     1261 ggatttgaag taggtggatg tgacatcggc catggaaaag cggaaatcga tggtttcatt
     1321 tggcaacagc ttctggcgca atgcggcggc caggccattc accggaatat ccacgttaaa
     1381 ggcaccttcg gtgatgcggc cggtttgaac attctcggtc acccaattcc gggatttcgg
     1441 aacgacgatg ggcggccaca gtttcttgag gaagtcagcc gagagatcgc gtacccggcc
     1501 actcaaccga atgcctgcgg actcgtcccc gccggtgata gttccgcgca agcggacgcc
     1561 ggcattgccc gacatcacca caagatcctc gatatccatc acggcgcctt cgaccgaagc
     1621 cttgccgagg aattcgatgc ggtcgacgag gaccggatat ttcacggtgc cttgcgtatc
     1681 gacgctgaca ttggtggcct tgaggtcaat cttgagatcc gtcaggcggc catcgcgggc
     1741 ccgcacggga tcaagacgcc cggtgagttc ggcgcgtgag ccgcccacca aaataatcga
     1801 gtcgataatc ttgaagccgc cagtgggcgc gtcatagtca acccgcaggg ctccttcatc
     1861 aatcacaatg ggctgggcga tgtagcctgg cagacccaca actccggccg cggcggcaaa
     1921 ttccgctgaa gcgctggtga ttacgccagc atcgctgatt tcgatttcgg cgtggcccga
     1981 cagcggcacg tcaaccttgg cga
//
LOCUS       DAORVN010000438         1996 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_61363_length_1996_cov_4.215868, whole genome shotgun sequence.
ACCESSION   DAORVN010000438 DAORVN010000000
VERSION     DAORVN010000438.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1996
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61363_length_1996_cov_4.215868"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..338
                     /locus_tag="PKV23_08700"
     CDS             <1..338
                     /locus_tag="PKV23_08700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HQX85553.1"
                     /translation="EESSLDAAWDLVKDWSAAERQALRDAVPRQGLKAMIRKRTVKDV
                     ARDMLKLSRAGLERRDRQGCKGKTEASFLDVLDETVATGRTAAENLLDLYHGPWKGNI
                     ERVFRDFAY"
     gene            413..1393
                     /locus_tag="PKV23_08705"
     CDS             413..1393
                     /locus_tag="PKV23_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002714620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HQX85554.1"
                     /translation="MFEGPGVFVLVILAVVVFYLLRAIRMVPQGYNYTVERFGRYVRT
                     LGPGLGLIVPFIDRIGAKMNMMERVIQVPSQEVITKDNAMVTVDGVAFYQVMDAPKAT
                     YEVVDLENAIINMVMTNIRTVMGSMELDSLLSQRDDINHKLLGVVDQATNPWGVKMTR
                     IEIKDINPPRDLVDSMGRQMKAEREKRASILEAEGLRQAAILKAEGEKQAVVLQAEGR
                     REAAFRDAEARERSAEAEAKATELVSTAIANGNVQAINYFVANNYVKALEAIAQSPNQ
                     KILMMPLDATSILGSIGGIAEIAREAFGGGSTETPKRKGASPVPGVTQKS"
     gene            1407..1853
                     /locus_tag="PKV23_08710"
     CDS             1407..1853
                     /locus_tag="PKV23_08710"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HQX85555.1"
                     /translation="MQDLIPLTGIWFWWIVAGVLLLFELMTPGVFAMWLALAAASVGI
                     ADYFIDLNWQLELLVFAGFSLLYVYLARPWYSRNKLQNSDQPNLNQRIYSFVGKSYVL
                     HEPIVDGEGKLDIEGTRWDVLGPDLARGKAVRVTAVEGMKLRVTEV"
BASE COUNT          414 a          567 c          604 g          411 t
ORIGIN      
        1 atgaagagag ttcccttgat gcggcttggg atctggtgaa ggactggagc gcggcggagc
       61 gccaggccct gcgcgatgcg gtgccccgcc aaggattgaa ggcaatgatc cgtaaacgga
      121 cggttaagga tgtggccagg gacatgctga agctatcccg cgccgggctg gagcggcgcg
      181 accgtcaggg ctgcaagggc aagaccgagg cctcgttcct cgacgtgctc gatgaaaccg
      241 tggcaaccgg caggaccgcc gccgagaacc tgttggatct ctaccatggc ccgtggaagg
      301 gcaatattga acgggttttt cgcgatttcg cctattgagg caacaaaagc cttgcaaatc
      361 acagaatgaa ttcctactta ggggtggttg gctgaactga aggagactga cgatgtttga
      421 aggtcctggc gtgtttgttc tggtaatcct tgccgtcgtt gtgttttacc ttctgcgggc
      481 catccgcatg gtgccgcagg gctacaacta cacggttgaa agattcggcc gctatgtccg
      541 cacccttggc cctggccttg gcctgatcgt tcccttcatc gaccgcatcg gcgccaagat
      601 gaacatgatg gagcgcgtca tccaggttcc gtcccaggaa gtcatcacca aggacaacgc
      661 catggtgacc gtggacggtg tggccttcta ccaggtcatg gatgctccca aggcaaccta
      721 cgaggtggtc gatcttgaaa acgcgatcat caacatggtg atgaccaata tccgcaccgt
      781 catgggctcc atggaactcg acagcctgct gtcgcaacgc gacgacatca accacaagct
      841 cctcggcgtt gttgaccagg ccaccaatcc ctggggcgtg aagatgaccc gcattgaaat
      901 caaggacatc aacccgcccc gcgatctcgt cgactccatg ggccgccaga tgaaggccga
      961 acgcgaaaag cgtgcctcga ttctcgaagc agagggcctg cgccaggccg ccattctcaa
     1021 ggccgaaggc gaaaagcagg cagttgtcct gcaggccgag ggccgaaggg aagcagcctt
     1081 ccgggatgcg gaagcccgcg aacgttctgc cgaggctgaa gccaaggcaa ccgaactggt
     1141 cagcaccgcc atcgccaacg gcaatgtcca ggccatcaat tattttgtcg ccaacaatta
     1201 tgtgaaggcg ctggaagcca tcgcccagtc gccgaaccag aagatcctca tgatgccgct
     1261 ggatgcaaca tccatactgg gttccatcgg cggcatcgcc gaaattgcca gggaggcttt
     1321 tggcggcggc tcgaccgaga cgccaaagcg caaaggcgcc tcgccggttc ccggcgtaac
     1381 ccagaaatcc tagggagcac caggccatgc aagatctgat tcccctgaca ggcatttggt
     1441 tctggtggat tgtcgccggc gtgcttctgt tgtttgaact gatgactccg ggcgtgttcg
     1501 ccatgtggct agcccttgcg gcggcctccg tcggcattgc cgattatttc attgatttga
     1561 actggcagct tgagttgctg gtttttgccg ggttctcgct gctctatgtc tatctcgcgc
     1621 ggccctggta ttcgaggaac aagctgcaga acagcgacca gcccaatctc aaccagcgca
     1681 tctacagctt tgtcggaaag tcctatgtcc tccatgagcc cattgtcgac ggtgagggaa
     1741 aacttgatat tgaaggcacc cgctgggatg tgctcgggcc ggacctggcc aggggcaagg
     1801 cggtccgggt gaccgccgtc gaaggcatga agctgcgggt cactgaagtc tagtcgtcgt
     1861 caacccgcca ttaaccataa cgccgctatt ttccggggtt tgaggagtgt ggcgacgtgg
     1921 ctttgggtct gcggctggtg tctgtatgcg ttgtggcagg ctttgctgcc accgcgcatg
     1981 gcgaaaccgc aggctt
//
LOCUS       DAORVN010000439         1996 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_61379_length_1996_cov_3.247810, whole genome shotgun sequence.
ACCESSION   DAORVN010000439 DAORVN010000000
VERSION     DAORVN010000439.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1996
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61379_length_1996_cov_3.247810"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..356)
                     /locus_tag="PKV23_08715"
     CDS             complement(<1..356)
                     /locus_tag="PKV23_08715"
                     /inference="COORDINATES: protein motif:HMM:NF012575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HQX85556.1"
                     /translation="MNSPLRPADLPRGNRGAFTVDPATSPTLPSRYYTDPKIYAAEMR
                     LIHQKSWCYVGHVCDVAEPGRYFTDTVGEQPILVVRGQDGVIRAFYNVCQHRGHELLK
                     GAGQLKTKGISCPYHA"
     gene            597..>1996
                     /locus_tag="PKV23_08720"
     CDS             597..>1996
                     /locus_tag="PKV23_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012385754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HQX85557.1"
                     /translation="MSTIRMTMAQALVRYLCNQFTTMDGARVPLFPGVFAIFGHGNVT
                     CLAEALEAVKDILPTWRGQNEQSMALAAIGFAKAKRRRQIMVATSSIGPGALNMITAA
                     GVAHTNRLPILILSGDTFVNRRPDPVMQQVEHYGDPTINVNDAFKAVTRYWDRIVHPE
                     QIISSLPQAVATMLDPADCGPAFIALPQDIQEYACDYPEAFFAPTVHEIPRPRPDRNS
                     LKKAVELLKKSRRPLIIAGGGVRYSGAEESVARFALAHGIPITETIAGKGTVIHSHDA
                     YAGAIGIVGSTSANALAAEADLVIAIGTRLMDFTTGSWTCFRQEAKFISINAARWDAT
                     KHQALAVVGDAKEAVDELAAALTGWKADTKWMAKARAEFAAWDKMLDGFQKPTNSPVP
                     TYAQIVGVVNAKASERDYAITASGGLPGEMMKNWRVKSANSFDVEFGFSCMGYEIAGG
                     WGAAMADPTRETFVMVG"
BASE COUNT          421 a          571 c          593 g          411 t
ORIGIN      
        1 caggcatggt atgggcacga gatgcccttg gtcttcagtt gtcccgcacc cttcaacaac
       61 tcgtggccgc gatgctggca gacattgtag aaggcgcgga tcaccccatc ctgccctctc
      121 accacgagga tgggctgctc gcccaccgtg tcggtgaaat accggccggg ctccgccaca
      181 tcgcagacat ggcccacata gcaccagctc ttctggtgaa tgaggcgcat ctcggccgcg
      241 tagatcttcg ggtcggtgta atagcgcgac ggcagcgtcg gcgatgtcgc cggatcaacc
      301 gtgaacgccc cgcgattgcc gcgcggcaga tcggcgggcc gaagggggct gttcatgtgt
      361 aatccaccac ggaaatttct cgctagggct tcagagaccg tcgaaacgga gcttatcagc
      421 ctttgcttgc tgcgcaaggc gtgcaaagcc gttgacctgg ttccccatga agatttcaag
      481 ttggccattt gaattatata gtggccattg cccaattgca cctgacactt gagatcatct
      541 ggaaggagtg cgagggttgg aagaatgcac atacataaga attgggagaa ttgggaatgt
      601 cgacgatccg catgactatg gcgcaggcac ttgtgcgcta tctttgcaac cagttcacca
      661 ctatggatgg cgcaagggtt ccgttgtttc ccggggtttt cgccattttt ggccatggaa
      721 atgtcacttg cctggccgaa gcgctggaag cagtgaaaga cattctgccc acgtggcgcg
      781 gccagaacga gcaatctatg gcgcttgcag ccatcggttt tgccaaggcc aagcgccgcc
      841 gccaaatcat ggtggcgaca tcctctatag gtcctggcgc gctaaacatg ataactgcgg
      901 ccggagtggc ccacaccaat cgcctgccga tcctgatcct gtcgggtgac acgttcgtca
      961 accggaggcc tgatccagtg atgcagcaag tcgagcatta cggcgatccc acgataaatg
     1021 tgaatgacgc tttcaaggct gtcacccgct actgggaccg catcgtgcat cccgaacaaa
     1081 tcatttcgtc actgccgcaa gccgtggcga cgatgcttga tcctgccgat tgcggccccg
     1141 cttttattgc ccttccgcag gatatacagg aatatgcctg tgactaccct gaagccttct
     1201 ttgcgccaac cgtccatgaa atcccgcgtc cgcgcccgga ccgcaacagt ttgaagaagg
     1261 cggtcgaact gctcaaaaaa tccaggcgcc cgctgatcat tgccggcggc ggcgtgcgct
     1321 attcaggggc ggaggaaagt gtagccaggt ttgccctggc acacggcatt ccgatcaccg
     1381 agaccatagc gggcaaggga accgtcattc acagccatga tgcctatgca ggcgccatag
     1441 gcatcgtggg ctcgacttcg gccaatgccc tggctgccga ggctgatctt gtcattgcca
     1501 ttggtacccg gctcatggac tttacgaccg gctcgtggac ctgcttcagg caggaggcga
     1561 aattcatttc catcaacgca gcgcgttggg atgcaaccaa gcaccaggcg cttgccgttg
     1621 tgggcgatgc gaaggaggcg gttgacgaat tggcagcggc gctcacaggc tggaaggctg
     1681 acacgaaatg gatggcaaag gcccgcgccg aatttgccgc atgggacaag atgcttgacg
     1741 ggtttcaaaa gccgacgaat tcgccggtgc cgacctatgc gcagatcgtt ggcgtcgtga
     1801 acgccaaggc cagcgaacgc gattatgcaa tcaccgcgtc cggcggcctt cctggcgaga
     1861 tgatgaaaaa ctggcgggtc aaatcagcca acagctttga cgttgagttt ggcttctcct
     1921 gcatgggcta cgagattgcc ggcggctggg gcgcggccat ggccgatccg acacgggaga
     1981 ctttcgtcat ggtggg
//
LOCUS       DAORVN010000440         1995 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_61442_length_1995_cov_2.691753, whole genome shotgun sequence.
ACCESSION   DAORVN010000440 DAORVN010000000
VERSION     DAORVN010000440.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1995)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1995)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1995
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61442_length_1995_cov_2.691753"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            62..757
                     /locus_tag="PKV23_08725"
     CDS             62..757
                     /locus_tag="PKV23_08725"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010913063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQX85558.1"
                     /translation="MRGIGLGIARRLNERGAKIAIWDRETPVWDETVKGFAPGLVIKT
                     DVSNVRSVDSAFQETMHRLGRIDILVNNAGINGPVLPAWDYPVEDWEKVIAIDLNGVF
                     FCTRSVLAHMRARKSGRIINVASIAGKEGVPGISAYSAAKGGVIAFTKALAKELAQDG
                     VLVNCIAPALVETDLFAEMTPEHIAAMRAKIPMGRLLQIEEVANMVSWIAGPECTFTT
                     GFAFDLTGGRATY"
     gene            complement(762..1361)
                     /locus_tag="PKV23_08730"
     CDS             complement(762..1361)
                     /locus_tag="PKV23_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019173779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HQX85559.1"
                     /translation="MSKPDYNSLSPDNIVAFLADIFERRGGEEYLGEPVTMAEHMLQG
                     AQFAENAGEPEIIIVAALLHDIGHFTSEFGSFTMEDVEDRHHEEAGAAVLERFFPTLV
                     TDCVRYHVAAKRYLCAARPEYLKRLSEASVLSLKLQGGPMSSVEVAEFEKNPNVKEIV
                     KVRHYDDAGKRTDMKTQPFSYYAPMVQRVVDAHCRKAAE"
     gene            complement(1358..>1995)
                     /locus_tag="PKV23_08735"
     CDS             complement(1358..>1995)
                     /locus_tag="PKV23_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015317288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HQX85560.1"
                     /translation="ETNYGKWFEVRTEVNPSNLAYTGLGLQAHTDNPYRDPVPTLQIL
                     YCLENSAKGGENMVVDGFKAATRLRAENPRGFELLSKHCARFEYAGSDGICLRARRPM
                     IELAPDGELIAIRFNNRSAAAVTDVPFDEMEAYYAAYRRLGEIIDDPAMEVSFKLSPG
                     ECFIVDNTRVLHARKGYSGAGSRWLQGCYADKDGLLSTLAAMEMPRQEAAA"
BASE COUNT          385 a          613 c          600 g          397 t
ORIGIN      
        1 cgctggagaa gccatgaccg agaaatacct ggaagggcag gtcgccattg tcaccggcgg
       61 catgcgcggc attggccttg gcattgcccg gcgcctgaat gagcgcggcg cgaaaattgc
      121 catctgggac cgcgaaacgc ccgtatggga tgaaactgta aagggctttg cgccgggcct
      181 tgtcatcaag accgatgtgt cgaatgtccg ctcggtagac agcgcattcc aagaaaccat
      241 gcatcggctt ggccgcatcg acatccttgt gaacaacgcc gggatcaatg gtccggttct
      301 ccccgcatgg gattatcccg tcgaggactg ggagaaggtc atcgccatcg atctcaatgg
      361 cgtgttcttc tgcacacgaa gtgtccttgc ccacatgcgc gccaggaaat ccggccgtat
      421 catcaatgtg gcgtcaatcg caggcaagga aggcgttccg ggaatctcgg cctattccgc
      481 cgccaagggt ggcgtcatcg ctttcaccaa ggcgctggcc aaggaactgg cgcaggacgg
      541 ggtactggtg aattgcatcg ctcccgccct ggtcgaaacc gatctcttcg ctgaaatgac
      601 gcccgagcat attgccgcca tgcgcgccaa gatccccatg gggcgcctgc tgcagatcga
      661 ggaagtggcc aacatggtga gctggattgc cgggcccgaa tgcaccttca cgacaggctt
      721 cgccttcgac ctgaccggcg gccgggcaac ctattgacga cttactccgc cgctttccgg
      781 caatgggcat cgacaacgcg ctggaccata ggggcgtaat aggaaaatgg ctgggtcttc
      841 atgtcggtgc gttttcccgc atcgtcataa tggcgcacct tcacgatctc cttcacgttc
      901 gggttctttt caaattcggc cacttcaacg gagctcatgg gcccgccctg cagttttagc
      961 gaaagaaccg aggcctcgga cagccgtttt aaatactcgg gcctcgccgc gcagagatag
     1021 cgcttggccg ccacatggta gcgcacgcaa tccgtgacca gggtagggaa gaaccgttcc
     1081 agcaccgccg ccccggcctc ctcgtgatgc cggtcttcca cgtcttccat ggtgaacgag
     1141 ccgaactcgc tggtgaaatg cccgatgtca tggagcagcg ccgccacgat gatgatctcc
     1201 ggctcgccgg cattctcggc gaactgcgcg ccctgcagca tgtgctcggc catggtaacg
     1261 ggctcgccca gatactcttc cccgccgcgg cgttcgaaaa tgtcggcgag gaaagccacg
     1321 atgttgtcag gcgaaaggga gttgtaatcc ggcttgctca tgcggcggcc tcctggcggg
     1381 gcatttccat cgccgcaagc gtcgacagca agccgtcctt gtcggcatag cagccctgga
     1441 gccagcgcga gccggcgccg gaataacctt tgcgggcatg cagcacccgg gtgttgtcca
     1501 cgatgaaaca ttcacccggc gacagtttga acgatacttc catggccgga tcgtcgatga
     1561 tctcgcccag ccggcgatag gcggcgtaat aggcctccat ctcgtcgaag ggaacgtcgg
     1621 tgaccgccgc tgccgaacgg ttgttgaagc ggatggcgat cagctcgcca tcgggcgcaa
     1681 gttcgatcat cgggcgccgc gccctgaggc agatgccgtc actgccggca tattcaaacc
     1741 gcgcgcagtg tttgctgaga agctcaaagc ctcttggatt ttctgcccgc agccgcgtgg
     1801 ccgccttgaa gccgtcaacc accatgttct cgccgccctt ggccgagttt tcgaggcagt
     1861 agagaatttg cagcgtgggc accggatcgc ggtagggatt gtccgtatgg gcctgcagcc
     1921 cgagcccggt ataggccaga ttgctcgggt tcacctcggt gcgcacttca aaccatttgc
     1981 cgtagttggt ctctc
//
LOCUS       DAORVN010000441         1993 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_61538_length_1993_cov_2.964912, whole genome shotgun sequence.
ACCESSION   DAORVN010000441 DAORVN010000000
VERSION     DAORVN010000441.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1993
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61538_length_1993_cov_2.964912"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1122)
                     /locus_tag="PKV23_08740"
     CDS             complement(<1..1122)
                     /locus_tag="PKV23_08740"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HQX85561.1"
                     /translation="MRTNRRLFLSTLGVWAASGLVGCGGGSSETPSAEETKPMGTWMA
                     VLSDLQEDVRFLDIPPPVSTSFRDQTLRQIAHLSMGGDQLRIKFGNLYGKAPLPLARV
                     RAALSTGGGAVDMATDVAVTFQGGDSVSIPPGAEVWSDKVSLRVPDGGDVAVSVYVRE
                     AADSTSSHRYANAIHYVASGDLSSAAAMPSSPANRLTSSYWMSAMDVYRARPARVVVA
                     FGDSLTDGNGSSMGANQRYPDLLSMRLLKTGGGSPVSVVNAGLGGNRWLHDRFGLRGV
                     ERFRRDALGVAGVTHAILQMGINDIGFQLAWTPNEKATAADLINSIASAVAAAKAMGI
                     QVYLTTMTPFKGHVYFSDPGEQIRQEVNAWIRTNTEVSGV"
     gene            1212..1874
                     /locus_tag="PKV23_08745"
     CDS             1212..1874
                     /locus_tag="PKV23_08745"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HQX85562.1"
                     /translation="MRIVCAGILPYVCASIATMPSTSSSPSLLIFAGSTRQQSFNRRL
                     AQQAAGMARAAGARVTLLELGDLDIPMYNADLEARGTPPDVLRLKRLMWEHPGWIICS
                     PEYNGSYTALLKNTIDWASSPVAADPEWKDGVKPFRGKVVGILSASNGRLGGLRSQSH
                     LAPLLLLLECWLAPRAYALAGAASAFDADGVLTQAEHRASVQGVVDQVLWATERLQAE
                     PR"
BASE COUNT          404 a          668 c          549 g          372 t
ORIGIN      
        1 gacacctgat acttcggtgt tcgtgcgaat ccaggcattc acctcttgcc gtatctgctc
       61 gcccgggtcg gaaaaataca catgcccttt gaagggcgtc atcgtcgtta ggtacacctg
      121 aatccccatc gccttggccg cagcaacggc actggcgatg ctgttgatca ggtctgcagc
      181 ggttgctttt tcattgggcg tccaggccaa ttgaaacccg atatcgttga tgcccatttg
      241 caaaatggca tgcgtcacac ccgctacacc cagcgcatcc cgccggaagc gctcgacccc
      301 tctcaagcca aaccggtcat gcaaccaacg attgcccccc aagcctgcat tgaccacaga
      361 aaccggggag cccccgcctg tcttgagcaa ccgcatggac aacaggtcgg gatacctttg
      421 gttggcgccc atgctggagc cattgccgtc ggtcaacgaa tccccgaaag cgactacgac
      481 acgggccggc cgcgccctgt acacatccat cgcactcatc caataagaag aagtgagccg
      541 attcgcgggg gacgacggca tggcagccgc gctgctcaaa tcaccggaag cgacgtagtg
      601 gatcgcgttc gcataccggt gtgacgaggt ggaatcggcc gcctccctca catacacact
      661 gactgccaca tccccgccgt caggcacccg aagcgacact ttgtcgctcc agacttcagc
      721 gccaggaggg atggacaccg aatcgccgcc ctggaacgtc acggcgacat ccgtcgccat
      781 gtccactgcc ccgccgcccg tgctcagcgc cgcacgaacc cgcgccaggg gcaatggcgc
      841 tttgccatac aggttgccga atttgatgcg cagctggtca ccgcccatgg acaagtgcgc
      901 aatttgcctg agggtctggt ccctgaagct ggtgctgaca ggtggcggga tatccaggaa
      961 acgcacgtcc tcttgaagat cactcaatac cgccatccat gttcccatgg gtttggtctc
     1021 ttcggccgag ggcgtttcgc tggaaccacc cccacagccg accagccccg atgcggccca
     1081 cacacccagt gtcgatagaa atagacgccg attggtccgc atccaggttc tcacagactg
     1141 cattgcttgt tcctattcat catgtacttg tgcgcgcaag tctatgggct tgccgccagg
     1201 caccgcacga tttgcgcatt gtttgtgcag gaatcctgcc gtacgtctgt gcttcaatcg
     1261 caaccatgcc cagtacctct tcttcgccct ccctcctgat cttcgcgggc agcacccgcc
     1321 aacaatcgtt caaccggcgc ctggcgcagc aagctgcggg catggcccgc gctgccggcg
     1381 ccagagtcac tctgctggaa ctgggcgacc tcgacatccc gatgtacaac gccgatctcg
     1441 aagcccgcgg cacaccgccc gacgtgcttc gcctcaagcg gctgatgtgg gagcacccgg
     1501 gctggatcat ctgctcgccc gaatacaacg gcagctacac cgcgctgctc aagaacacca
     1561 tcgactgggc gtcgagcccc gtggcggcag accccgaatg gaaagatggc gtcaagccgt
     1621 ttcgcggcaa agtggtgggc atactcagcg catccaatgg gcgcctgggc ggcctgcgct
     1681 cccaaagcca cttggcgcct ttgctgctcc tgctcgaatg ctggctggcg ccgcgcgctt
     1741 atgcactggc tggcgccgcc agcgcgttcg acgcggacgg cgtgctgaca caggcagagc
     1801 accgggcgag cgtacaaggc gtggtcgatc aggtgctctg ggctacagag cggctgcaag
     1861 ccgagccacg ctgaaaaatt caaacaaaat gagcctctgg cgcttactgc acaaacatca
     1921 acagctatca aaatgatagt cgaataaatt catggattgc cgccccggct gcggcgcctg
     1981 ttgcaccgcc cct
//
LOCUS       DAORVN010000442         1991 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_61646_length_1991_cov_3.631198, whole genome shotgun sequence.
ACCESSION   DAORVN010000442 DAORVN010000000
VERSION     DAORVN010000442.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1991)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1991)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1991
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61646_length_1991_cov_3.631198"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            94..834
                     /locus_tag="PKV23_08750"
     CDS             94..834
                     /locus_tag="PKV23_08750"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HQX85563.1"
                     /translation="MKTTPRLHLEAPLSAGSEINLSREQGHYLTSVLRLSSGDALRAF
                     NGRDGEWLAYLATVSKKSVTLRCERRVAEAKLPPDIDYVFAPLKHARLDYVVQKATEL
                     GARRLRPVMTSRTVAERVNLERMRANVVEAAEQCNLVHLPEVLEPEKLEKVLAGWDKG
                     RSLVYCDETMVDRNPLESLRSLRQPSAVLVGPEGGFTDAEKALLKSRTFVIPISLGPR
                     IMRADTAAIAALTLMQALAGDWGTHDIS"
     gene            917..>1991
                     /locus_tag="PKV23_08755"
     CDS             917..>1991
                     /locus_tag="PKV23_08755"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012116172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HQX85564.1"
                     /translation="MSGPSPDTQEARAPIENKAQLIGALSRGTKPKADWRIGTEHEKF
                     PFLTDTLKPVPYDGPRSIRALLEGLRDRFGWQGVYENDKIIALSDPKGMGNVSLEPGG
                     QFELSGAPLQSVHDTCEEVNEHLVQVREIGDALGIGFLGLGASPTWTRAETPIMPKGR
                     YQIMAPYMDKVGRYGRDMMFRTCTVQVNLDFGSEADMVKKLRVSLALQPVATALFANS
                     PFLEGKPNGFLSFRSEVWTDTDKARAGMLPFAFEPGMGFERYVDYALDVPMYFVMRDG
                     KYLNTAGESFRKFLEGKLPQLPGEKPVAKDWADHLTTIFPEVRLKQYLEMRGCDSGPW
                     RRLCALPALFTRLLYDQGALDAAW"
BASE COUNT          400 a          653 c          567 g          371 t
ORIGIN      
        1 aaggcaacag gcgctcaacc caattgtcgc ggcccgcatc ggcaatgtga tggctggtct
       61 ccgtcatgtg ccccgtgtta catcaggctc gacatgaaaa ccacaccccg ccttcacctg
      121 gaagcacccc tctcggccgg aagcgaaatc aacctcagcc gcgagcaagg ccactatctg
      181 accagcgtgc tgcgcctgtc ttcgggcgat gcgctgcgcg ccttcaatgg ccgggatggc
      241 gaatggctcg cctatctcgc gaccgtcagc aagaaatccg tgacgctgcg ctgtgagcgg
      301 cgcgttgccg aagcgaaact gccgcccgac atcgattatg tcttcgcacc cctcaagcat
      361 gcgcgcctgg attacgtggt gcagaaggcc accgaactgg gcgcccgccg gttgcgcccc
      421 gtcatgacca gccgcaccgt ggccgagcgc gtcaatcttg agcgcatgcg cgccaatgtg
      481 gttgaggccg ccgagcagtg caacctggtc catcttcccg aagtgctgga accggaaaaa
      541 cttgagaagg ttctggccgg ttgggataag ggcaggtcac tggtctactg cgatgaaacc
      601 atggttgacc gcaatcccct ggaaagcctc aggtccctgc gccagccatc agccgttctg
      661 gtgggcccgg aaggtggttt caccgacgcg gaaaaagccc tgctcaaatc ccgcaccttt
      721 gtcattccca tctccctggg gccgcgcatc atgcgcgccg atacggcggc catcgcggcc
      781 ctgaccctta tgcaggccct ggctggagac tggggcaccc atgatatttc ctgatggatg
      841 gacgcacaat ctttggcttg ccggaacatg gcgcaccgcc taaatccaag gcaaatcggc
      901 aacaaggatt gatacagtga gcggaccaag tcccgatacc caggaagcgc gcgcgccgat
      961 tgaaaacaaa gcccaactca tcggcgcctt gagcaggggc accaagccca aagcggactg
     1021 gcgcatcggc accgaacacg agaaatttcc gttcctcacc gatacgctga agcccgtgcc
     1081 ctatgacggc ccccgctcca tccgcgccct gctcgaaggc ctgcgcgacc gctttggctg
     1141 gcagggcgtc tatgagaatg acaagatcat tgccttgagt gatcccaagg gaatgggcaa
     1201 tgtttcgctg gagccgggcg gccagttcga gctctccggc gctcccctcc aatccgtcca
     1261 cgatacctgc gaagaagtga atgagcattt ggtccaggtc agggaaattg gcgatgcgct
     1321 gggcatcgga tttctcggcc ttggcgcctc gcctacctgg acgcgcgccg aaaccccgat
     1381 catgcccaag ggccgctacc agatcatggc gccctacatg gacaaggttg gccgctatgg
     1441 ccgcgacatg atgttccgga cctgcaccgt gcaggtcaac ctggatttcg gctccgaagc
     1501 cgacatggtg aagaagctcc gtgtttcgct ggccctgcaa cccgtggcca ccgcactgtt
     1561 tgccaactcg ccctttcttg aaggcaagcc caatggtttc ctctctttca gatcagaagt
     1621 ctggaccgat accgacaagg cccgcgccgg catgctgccc ttcgccttcg agccgggcat
     1681 gggttttgaa cgctatgtgg actatgccct cgacgtgccc atgtattttg tcatgcgtga
     1741 cggcaaatat ctcaacacgg cgggcgaaag cttccgcaag ttcctcgaag gaaaactgcc
     1801 gcagctgccc ggcgaaaaac ccgtggccaa ggactgggcc gatcatctca ccaccatctt
     1861 cccggaagtg cgcctgaagc agtatctcga aatgcgcggc tgcgactccg gcccatggcg
     1921 ccggctttgc gcactgcccg ccctgttcac ccgcctgctc tatgaccaag gcgcactcga
     1981 tgccgcctgg g
//
LOCUS       DAORVN010000443         1991 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_61664_length_1991_cov_2.860021, whole genome shotgun sequence.
ACCESSION   DAORVN010000443 DAORVN010000000
VERSION     DAORVN010000443.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1991)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1991)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1991
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61664_length_1991_cov_2.860021"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(53..478)
                     /locus_tag="PKV23_08760"
     CDS             complement(53..478)
                     /locus_tag="PKV23_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010914269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diol dehydratase small subunit"
                     /protein_id="HQX85565.1"
                     /translation="MTAKLTVADYPLAETRSDRIKGKRGKSLDDITLESVVAGRVTME
                     DLRITPQALADQAEISRAAGRPTLALNFERAAELVGVPQDFIMSSYEMLRPGRAKSRD
                     QLLEVANTFRQVYKAERMAKFIEEAADTYERRGLFTYRF"
     gene            complement(475..>1991)
                     /locus_tag="PKV23_08765"
     CDS             complement(475..>1991)
                     /locus_tag="PKV23_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019859775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="propanediol/glycerol family dehydratase large
                     subunit"
                     /protein_id="HQX85566.1"
                     /translation="GIPTISTFSVGTSGAGSELLMGFHESKSLLYLEARCLCLQRGMG
                     AQGTQNGGIDGAPLTATVPGGIRELMAENLLAVWLDLECASGNDARSTESEIRVGAKI
                     LPFLISGSDLICSGFGSILAYDNSFNPSLINGEEMEDFLVLQRDFEADGGLTPLPEER
                     ALDLRSRAVEAISAVFEELGLSTPTSDMKQSVVVASGSDETRSYTPRDVAKISEAIKE
                     REITVIEVIMALVKRGFADEAQNLLSLVKLRVSGDYLQTSAMVRKCKVVSAVNDPNTY
                     LGPGTGYRVSEERRLELNSIRDVLDQKEVLRQEALHEKDEAKRIQYRVKGPAGQGTDK
                     KEVVIGISPAFGLNLFQTTAGHRLSDVLKGMIDAIEKRGLKPRIVRFRHTADTSFLGI
                     SAATLSGSGIGIGIQAKGTAVIHSRERLPHNNLELFSNAPITRLEHYKGFGANAAAYA
                     LGEMPEPVVVPTRGEAMGSRYHARVALIYAIETGLTAEGAAPEDIEVKFTGVKS"
BASE COUNT          379 a          571 c          632 g          409 t
ORIGIN      
        1 caccttcgta atagatcgaa cgataaccag ttttccacga catggcgtga tcctaaaagc
       61 ggtaggtgaa gaggccgcgg cgttcataag tgtcggctgc ctcttcaata aattttgcca
      121 tgcgctcggc cttgtaaacc tggcggaatg tgttcgccac ttcgagaagc tgatccctgg
      181 attttgcccg gccggggcgc agcatctcgt aggaactcat gatgaaatcc tggggcacgc
      241 ccaccaattc agcggcgcgc tcaaaattca gggccagagt ggggcgcccg gcggcgcggg
      301 aaatttcggc ctggtcggcc agtgcctggg gggtgatgcg cagatcttcc atggtcaccc
      361 tgcccgccac gacggattcc aaggtaatgt catcgaggga cttgccacgc ttacccttga
      421 tcctgtcgct tcgggtttcg gccaggggat agtccgcgac agtgagcttt gcagtcatga
      481 tttaaccccg gtgaacttca cttcgatatc ctctggcgcg gcaccttccg ccgtcagtcc
      541 ggtttcgatg gcgtagatca gggccacacg tgcgtggtag cgcgagccca tggcttcacc
      601 gcgcgtcggc accaccacgg gttcgggcat ctcgcccaga gcgtaagctg cggcattggc
      661 gccaaagccc ttgtagtgtt cgagcctggt gatgggggcg ttggaaaaga gttcgagatt
      721 gttgtggggc aagcgttccc ggctatggat gacggcggtg cccttggcct gaataccaat
      781 gccgatgccc gagcctgaca gggttgccgc ggagatgccg aggaatgacg tgtcagccgt
      841 atgcctgaag cgcacgatgc ggggcttgag gccgcgcttt tcaatggcgt cgatcatgcc
      901 tttgaggacg tcggagagac gatggcccgc cgtggtctgg aacaggttca ggccgaaggc
      961 cgggctgatg ccgatcacca cttccttttt gtccgtcccc tgcccggctg gacccttgac
     1021 gcggtactgg atgcgtttgg cctcgtcctt ttcatgcagc gcttcctggc gcagcacttc
     1081 cttctggtcc agtacgtcgc ggatggaatt gagctcaagg cggcgctcct cagagacgcg
     1141 gtagccggtg ccggggccga gataggtgtt gggatcattc acggcgctga caaccttgca
     1201 cttccgcacc atggcggagg tctgcaggta atcgccggag acgcggagct tcacgaggga
     1261 cagcaggttc tgagcctcat cggcaaagcc gcgtttcacc aatgccatga taacttcaat
     1321 tacggtgatc tcgcgctcct tgatggcttc gctgattttc gccacatcgc gcggcgtgta
     1381 gctgcgggtt tcatcggagc cggaagccac aaccacactt tgcttcatgt ctgacgtggg
     1441 ggtgctcaaa cccagttcct cgaacacggc ggaaatggct tcaacggcgc ggctgcgcaa
     1501 atcgagggcg cgttcttccg gcagcggcgt caagccgcca tcggcctcga agtcgcgctg
     1561 cagcacgaga aagtcttcca tctcctcgcc attgatcagc gaaggattga aggaattgtc
     1621 ataggccagg atggagccaa agccggagca gataaggtcg gagcccgaaa tcaggaaggg
     1681 caggatcttg gcaccgacgc ggatttcgga ctcggtcgag cgcgcgtcat tgccggaagc
     1741 gcattccagg tcgagccaga cggccagaag attctccgcc atcaattcgc ggatgccgcc
     1801 gggaacggtt gcggtcagcg gcgcaccatc aatgccgccg ttctgggtgc cttgcgcacc
     1861 catgccgcgc tgcaggcaga ggcatcgggc ttcgaggtaa agcagcgact ttgattcatg
     1921 gaagcccatg agcagttcgg aaccggcgcc ggaggtgccg acgctgaagg tggaaattgt
     1981 gggaatgccc g
//
LOCUS       DAORVN010000444         1990 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_61729_length_1990_cov_2.490956, whole genome shotgun sequence.
ACCESSION   DAORVN010000444 DAORVN010000000
VERSION     DAORVN010000444.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1990
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61729_length_1990_cov_2.490956"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(139..345)
                     /locus_tag="PKV23_08770"
     CDS             complement(139..345)
                     /locus_tag="PKV23_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012454999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HQX85567.1"
                     /translation="MRYAIVIEKAGSNYSAYVPDLPGCIATAATIPEVESEIRDAIRF
                     HLEGLKEDGFTPPFTTSIAEYVDA"
     gene            complement(345..542)
                     /locus_tag="PKV23_08775"
     CDS             complement(345..542)
                     /locus_tag="PKV23_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013504344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HQX85568.1"
                     /translation="MHKVREVISMIEGNGWRLVATRGSHRQYKHPEKPGRVTIAGKMS
                     DDMAPGTFNSIMKQAGLKGRD"
     gene            866..>1990
                     /locus_tag="PKV23_08780"
     CDS             866..>1990
                     /locus_tag="PKV23_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012653209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HQX85569.1"
                     /translation="MFAALASTAIAETKLTIATVNNGDMIRMQGLTDDFTKANPDITL
                     EWVTLEENVLREKVTTDIAAKGGQFDVLTIGTYEVPIWGKKGWLVPLTAVDDPADILP
                     AIAGGLTVDGKLMAAPFYGESSMVMYRKDLMEKAGLTMPDAPTWEFIGQAARAMTDKA
                     AETYGVCLRGKAGWGENMAFLTATSNSFGARWFDENWKPQFDQPEWKATLDYYLSLMA
                     DAGPPGASSNGFNENLALFNSGKCGMWIDATVAASFVTGKDSTVADKVGFALAPDNGL
                     GKRGNWLWAWSLAIPAGTQKNDAAQKFVGWATSKQYTALVAEKEGWANVPPGTRTSLY
                     ENAEYAKVPFAKMTLDSINSADPTKPTVKPVPYVGVQFVAI"
BASE COUNT          467 a          577 c          508 g          438 t
ORIGIN      
        1 atgtcacttg gatcaacgga tgtggcgaat atatccagcc accaagacaa tactttgtag
       61 gctaaacagg gaaggatcgt gccacacaat ctctatacaa agaaattgcg ccagggctct
      121 tattgaagtt gcgtaaaatt acgcatccac atactcagca atgcttgtcg taaatggagg
      181 ggtgaagcca tcttccttca ggccttccag atgaaagcga atcgcgtccc tgatctcgga
      241 ttcgacttct ggtatagttg ccgctgtagc gatgcagccg ggcaggtccg gtacataggc
      301 tgaataattt gaaccagcct tttcaataac gattgcgtag cgcatcagtc tcttcccttc
      361 aaacctgctt gcttcattat actgttaaaa gttccgggtg ccatgtcatc gctcattttc
      421 cctgcaatag ttacccgtcc cggcttttcc ggatgcttgt attgcctatg gcttcctcgt
      481 gttgccacca aacgccagcc attgccctca atcatggaaa tgacctcgcg gaccttatgc
      541 attcatgccc ccaagcctgc caacacagtc aacgtttagt cgctttccac agaataggga
      601 gtgaatttca agttgccact cagatcaaaa aattacctga gcttttgctt aaataaaatg
      661 caaatgcctg agaccgatta atcgcattta tttcaattag ttaaaccaag aactgcatcc
      721 cgtttgtgca ttgcggcgac gaattcctat tgactccgcc agggttaaga ttgagtatat
      781 gcccgcttag ttggcaaaag ctcaacagaa tgcatttggg aggaattcaa tgaaacccgt
      841 cttaacaggc gtctttggcg ctttcatgtt tgccgccttg gcctcaaccg ccatcgccga
      901 aaccaaactc accatcgcca ccgtgaacaa tggcgacatg atccgcatgc agggtctgac
      961 cgatgatttc accaaggcca atccggatat caccctggaa tgggtcaccc ttgaagaaaa
     1021 cgtgctccgt gaaaaagtga ccaccgacat cgccgccaag ggcggccaat tcgacgtgct
     1081 caccattggc acctatgaag ttccgatctg gggcaagaag ggctggctcg tgccattgac
     1141 ggccgttgat gacccagcag acattctgcc cgccatcgcc ggcggcctca cggttgacgg
     1201 caagctcatg gcagcgcctt tctacggcga aagctccatg gtcatgtatc gcaaggactt
     1261 gatggaaaag gcaggcctca ccatgcctga tgcgcccacc tgggagttca tcggccaggc
     1321 agcccgcgcc atgactgaca aggcggcgga gacctacggc gtgtgcctgc gcggcaaggc
     1381 cggctggggc gagaacatgg ccttcctcac cgccacctcg aattccttcg gggcccgctg
     1441 gtttgacgaa aactggaagc cccagttcga tcagccggaa tggaaggcga ccctggacta
     1501 ttatctcagc ctgatggctg atgccggccc tccaggcgca tcgtccaacg gcttcaacga
     1561 aaacctggcc ctgttcaact ccggaaaatg cggcatgtgg attgatgcca cggttgcggc
     1621 ctccttcgta accggcaagg actcgactgt tgctgacaag gtaggcttcg cgctggcgcc
     1681 ggataacggc ctcggcaagc gcggcaactg gctgtgggcc tggtcgctgg caattcccgc
     1741 cggcacgcaa aagaacgacg ccgcccagaa gtttgtcggc tgggccacca gcaagcagta
     1801 caccgctttg gtagccgaga aggaaggctg ggccaacgtt cctcccggca cccgcacttc
     1861 gctctacgag aacgccgaat atgccaaggt gccatttgcc aagatgaccc tggactcgat
     1921 caattccgcc gacccgacca agccgacggt aaagccggtg ccttacgtcg gcgtgcagtt
     1981 cgtggccatt
//
LOCUS       DAORVN010000445         1988 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_61824_length_1988_cov_3.734092, whole genome shotgun sequence.
ACCESSION   DAORVN010000445 DAORVN010000000
VERSION     DAORVN010000445.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1988)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1988)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1988
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61824_length_1988_cov_3.734092"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..904)
                     /locus_tag="PKV23_08785"
     CDS             complement(<1..904)
                     /locus_tag="PKV23_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF255 domain-containing protein"
                     /protein_id="HQX85570.1"
                     /translation="MSHNRLSLETSPYLLQHKDNPVHWMGWSEQALQEARDRDQPILL
                     SVGYAACHWCHVMAHESFEDPVTAGLMNQNFINIKVDREERPDIDRIYMAALHSLGEQ
                     GGWPLTMFLTPDALPFWGGTYFPPETKYGRPSFRHVLTEISRIWQQERDKVTNNAEAI
                     SAALQQPRYEASPNQLSSAEIHQAADVIINATDPHYGGLKGAPKFPQAPIFTFLWEAY
                     LRSRNEKARDAVLTTLTQISNGGIYDHLAGGIARYSTDPKWLAPHFEKMLYDNAQYVS
                     LLTRAWLKTESPLYATRVEQTIEFI"
     gene            925..>1988
                     /gene="mnmE"
                     /locus_tag="PKV23_08790"
     CDS             925..>1988
                     /gene="mnmE"
                     /locus_tag="PKV23_08790"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF003661.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HQX85571.1"
                     /translation="MNNSETIFAVSSGSGMAGITVIRMSGGKAGGVLASVAGPLPLPR
                     RASIRQLRHPQTQEVIDQGLVLWMPGPMTSTGEDVAEFHVHGSAAVIEAMFEVFRSIS
                     GVRLAEPGEFTRRAFVNNRMDLVEAEGLADLLQARTEAQRRMAMHHMLGHASSQYEDW
                     RTEMISLLARLEAAIDFVEEAGVAVAALADIRPKTLDLVARLSRAAAEADRAGALRSG
                     VKVVFAGAPNVGKSTLLNLVAAREAAIVSSRPGTTRDVIEVPVVLGGIPVILTDTAGL
                     RAKAGDEVEVIGMQRAKSEAAAADIVVWVSSVDVAEDTENAVVPALEVLNKSDLPGDA
                     HGADLRVSARTGEGIAELIA"
BASE COUNT          372 a          510 c          659 g          447 t
ORIGIN      
        1 ggataaattc aatcgtttgt tcaacgcgtg ttgcgtagag cgggctttcg gttttgagcc
       61 aggcgcgggt gagcaggctc acatattggg cgttgtcgta gagcattttt tcgaaatgcg
      121 gcgccagcca tttggggtca gtggagtagc gcgcgatgcc gccggccaga tgatcataga
      181 ttccgccatt gctgatctgc gtgagcgtgg tgaggaccgc gtcgcgcgcc ttctcattcc
      241 ggctgcggag ataggcttcc cacagaaatg tgaagatggg ggcttgggga aattttggcg
      301 cgcccttgag gccaccatag tgtggatccg tggcgttgat gatgacgtcg gctgcctgat
      361 gaatttccgc actggagagc tggttcgggc ttgcctcata tcgcggctgt tgcagggcgg
      421 cagatatggc ttcggcgttg ttcgtgactt tgtctcgttc ctgctgccag atgcgcgata
      481 tttcagtcag gacgtggcgg aaactgggcc gcccgtattt tgtctcaggg ggaaaataag
      541 tgccacccca aaagggaagg gcatcggggg tcaggaacat ggtgaggggc cagccgccct
      601 gttcgccgag gctgtgaagg gccgccatgt agatgcggtc gatatcagga cgctcttcac
      661 gatcgacctt tatgttgatg aagttctggt tcataagacc ggcggtgaca ggatcctcga
      721 aactctcgtg ggccatcacg tggcaccagt ggcaagcggc atagcctacg gacagcagaa
      781 tgggctggtc gcggtccctg gcctcctgaa gcgcctgttc actccatccc atccagtgaa
      841 ctggattgtc cttgtgctga aggagatagg gactggtttc caggctgaga cggttatgtg
      901 acatggtatc gattcgaatc cgaaatgaat aattctgaga caattttcgc ggtttccagc
      961 ggctccggta tggccggaat taccgtcatc cgtatgtccg gtggcaaggc cggaggcgtg
     1021 cttgcatccg ttgcaggtcc cctaccctta ccgcggcggg ccagcatccg gcaactgcgg
     1081 catccccaga cccaggaagt catcgaccag ggcctcgtcc tttggatgcc tgggccgatg
     1141 acttcaactg gtgaagacgt ggctgagttc catgtgcacg gtagtgccgc ggtgatcgag
     1201 gcgatgtttg aggtcttccg ttccatctct ggggtgcggc tggcggagcc cggcgaattc
     1261 acccgtaggg cctttgtcaa taatcggatg gatctggtgg aagcggaggg acttgcagac
     1321 ctgctccagg cccgcacgga ggcgcagcgg cgtatggcca tgcaccacat gctcggtcat
     1381 gccagttcac aatatgagga ctggagaaca gagatgatct cgcttctggc gcgccttgaa
     1441 gcagcgattg atttcgtgga agaggctggc gtggccgttg ccgcacttgc tgatatcagg
     1501 cccaagacac ttgacctggt agcgcgtctc agtagggccg cggcggaggc agatcgtgct
     1561 ggcgctctgc gctcgggggt gaaagttgtt tttgccggtg cgccgaatgt cggtaaatcg
     1621 acgctgctga atttggttgc cgcgcgggaa gcagcaatcg tgtcttcgcg tcccggcact
     1681 acccgcgacg tgatagaggt tcccgttgtg ctgggtggca ttcctgtcat actgacggat
     1741 acggcgggcc ttcgcgccaa ggccggtgac gaggttgaag tcatcggcat gcagcgcgcc
     1801 aagtcagaag ccgccgctgc cgacattgtt gtctgggtga gttcggttga cgtggcagag
     1861 gacactgaaa atgcggtagt gccggcactc gaggttttga acaagtcgga cctaccgggg
     1921 gatgcccatg gagcagactt gcgagtttcg gccaggaccg gagaaggcat cgcggaactc
     1981 attgccga
//
LOCUS       DAORVN010000446         1984 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_62047_length_1984_cov_3.113012, whole genome shotgun sequence.
ACCESSION   DAORVN010000446 DAORVN010000000
VERSION     DAORVN010000446.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1984)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1984)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1984
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62047_length_1984_cov_3.113012"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..171
                     /locus_tag="PKV23_08795"
     CDS             <1..171
                     /locus_tag="PKV23_08795"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013501014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HQX85572.1"
                     /translation="VPAMTPLAEEISKDMKKRGFRFVGPTIVYAFMQACGLVNDHMID
                     CHRHEAVKKLAR"
     gene            173..775
                     /locus_tag="PKV23_08800"
     CDS             173..775
                     /locus_tag="PKV23_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011580298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD family hydrolase"
                     /protein_id="HQX85573.1"
                     /translation="MAAELRAWQRMLSGRRLDLLDPSPLDIEIEDIAHGLARVARWNG
                     QTKGDHAFSVAQHCLLVEGIAAHVNRQLDRPSRLMALLHDAPEYVVGDMISPFKAAIG
                     LDYKAFEKRLLSAIHMRFSLPPKTPAACEAEIKAADKISAYLEATQLAGFSVPEAEKF
                     FGRPKGLSGEGSRKFFQLKPLPPNDAAAMFLKRFKLLVQP"
     gene            772..1275
                     /locus_tag="PKV23_08805"
     CDS             772..1275
                     /locus_tag="PKV23_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013531123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein tyrosine phosphatase"
                     /protein_id="HQX85574.1"
                     /translation="MIIVCGLHAVQELVDTHAVGRVVSLLGPDTPHQAFNNILAEQHL
                     KLTFHDVVEMMDGFSAPRATDAEKLIEFIEGWDQSRPMLIHCWAGISRSTASAYTALC
                     MLRPSADEEELAQELRAASPSATPNRLIVSYADALLGRNGRMSRAIEKIGRGENAYLG
                     RPFVLRP"
     gene            complement(1277..>1984)
                     /locus_tag="PKV23_08810"
     CDS             complement(1277..>1984)
                     /locus_tag="PKV23_08810"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014285083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HQX85575.1"
                     /translation="DLELHCQRLICSARSFDLKILKNAEEIEAIIREGIARFPKTAEL
                     YLRPMYWAAAGFVDVDPESTKFSVSVYDSPLPKPTSSLSITLSPFRRPSNEYAPTDAK
                     AACHYPNSARAIRDAKARGFDNAVMLDPLGHVAELTTANIWCAKNGEAYTPVPNGCFL
                     NGITRQRIVSLLRGAGIKVHECSLDWKDLLDADEVFSTGNYSKVMPVTTIEQRTLQPG
                     PIAAKARELYWAYAHGK"
BASE COUNT          389 a          551 c          637 g          407 t
ORIGIN      
        1 gttccggcca tgacgccgct ggcggaggag atctccaagg acatgaaaaa gcgtggtttc
       61 cgctttgtcg ggcccaccat cgtatatgcc ttcatgcagg cttgcgggct ggtcaatgac
      121 cacatgattg actgccaccg ccatgaggcg gtgaagaaac tggcgcgctg agatggcagc
      181 ggaacttcgc gcctggcagc ggatgctttc ggggcgcagg cttgacctgc tggacccctc
      241 gccgctcgac atcgagatcg aggacatcgc ccatgggctg gcacgggtgg cccgctggaa
      301 tggccagacc aagggcgacc atgccttttc cgtggcgcag cattgccttt tggtcgaagg
      361 cattgcggcc catgtcaacc ggcagctgga caggccgtcg cggctgatgg cgctgctgca
      421 cgatgcgccg gaatatgtgg tgggcgacat gatctcgccc ttcaaggcgg ccatcgggct
      481 tgattacaag gcctttgaaa agcgcctgct ttcggccatt catatgcgct tcagcctgcc
      541 gcccaagacg cccgctgcat gtgaagctga aatcaaggcg gcggacaaaa tatcggccta
      601 tctggaagcc acgcagctcg ccggtttttc cgtgcccgag gcagaaaaat tttttggccg
      661 gcccaagggg ctgagcggcg agggttcgcg gaaattcttt cagctcaagc ccttgccacc
      721 caatgatgca gcggccatgt tcctcaagag gttcaaattg ctggtgcagc catgatcatc
      781 gtttgcggtc tccatgccgt gcaggaactg gttgatactc acgcggtggg gcgggttgtg
      841 agcctgctcg ggcccgatac gccgcaccag gcgttcaaca acattctggc ggaacagcac
      901 ctgaagctga cttttcacga cgtggttgag atgatggatg gcttttcagc gccgcgggca
      961 acggatgcgg aaaagctgat cgagttcatc gagggctggg accagtccag gcccatgctg
     1021 atccattgct gggcgggcat cagccgctcg acggcttcgg cctatacagc cctttgcatg
     1081 ctgcggccat cggccgatga agaggaactg gcccaggagt tgcgcgcagc ctcgccttcg
     1141 gccacgccaa accgcttgat cgtttcctat gcggatgcgc ttctcgggcg caacggccgc
     1201 atgtcgcggg ctatcgagaa gatcgggcgc ggcgaaaacg cttacttagg caggccgttc
     1261 gttctgcggc catgaatcat tttccgtggg cgtaggccca gtagagttcg cgggcctttg
     1321 cggcgatggg gccgggctgc agggtgcgct gttcgatggt cgtcaccggc atgaccttgc
     1381 tgtagttacc ggttgagaaa acctcatcgg cgtcgagaag gtccttccag tcgaggctgc
     1441 attcatgcac cttgatgccc gccccccgca aaagggaaac aatgcgctgg cgggtgatgc
     1501 cattgaggaa acagccgttt ggcaccggcg tataggcctc gccattcttg gcgcaccaga
     1561 tattggcggt ggtgagttct gccacatggc ccaggggatc aagcattacg gcattgtcaa
     1621 agcccctcgc cttcgcatcg cggatggcgc gggcggaatt gggatagtgg caggcggcct
     1681 tggcgtcggt gggggcatat tcattggaag gccggcggaa aggcgacagg gtaatcgata
     1741 gggatgacgt gggcttgggc aacggtgaat cataaaccga gaccgagaat ttggtggatt
     1801 cggggtcgac atcaacaaag cctgcggcag cccagtacat gggacgcaaa tagagctcgg
     1861 cggttttcgg gaacctggcg atgccttcac ggataatcgc ttcgatttcc tcggcattct
     1921 tcaggatctt caagtcaaaa cttctggcag aacagatcag gcgctggcaa tggagctcga
     1981 gatc
//
LOCUS       DAORVN010000447         1984 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_62069_length_1984_cov_2.366511, whole genome shotgun sequence.
ACCESSION   DAORVN010000447 DAORVN010000000
VERSION     DAORVN010000447.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1984)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1984)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1984
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62069_length_1984_cov_2.366511"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1279
                     /locus_tag="PKV23_08815"
     CDS             <1..1279
                     /locus_tag="PKV23_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HQX85576.1"
                     /translation="QLIENAGRAVAEEITRRFGARRTVVLCGPGNNGADGKVAARYLK
                     QWGWPVTIADGIEGAELIIDALYGAGLNRDFPAALADKINTAGVPIVSVDVPSGLDGL
                     TGHPRGSCVKADLTITFFRKKPAHLLLPGRMLCGEVVVADIGIPATVLPELKIHLFEN
                     SKPFLPAINCASHKYKRGHAVVVSGAKFNTGASRLAAQAALKIGAGLVTIAGLQDELL
                     VHAAHTTAIMLAQTNTALALGMFLNDARRNAVCIGPAASVGSETCARVRAILASGAAT
                     VLDADALTSFARNPAELFAAIAERPRRPVILTPHEGEFARLFKTLATAPEAKHERALK
                     AAKSAGAIIILKGADTVIASPDGRAAINCNAPPSLATAGSGDVLAGLATGLLAQGMPA
                     FEAACAAVWLHGEAANRHGPNGLTAETLLSALI"
     gene            1325..>1984
                     /locus_tag="PKV23_08820"
     CDS             1325..>1984
                     /locus_tag="PKV23_08820"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HQX85577.1"
                     /translation="MNRPLFLLLGTGAALGLNFPIGKMAMAAGVTPALWAAVISAGAG
                     LAMLFIVAVTERGDLSRTSTLSFAIISGFLSYVVPNLLTYSVIPKIGSGLAAIMFALS
                     PVVTALLSVVLRVRPPNLLGIFGITLGLAGAGIIIFSRQLDFSGSTPWILLALLIPVF
                     LGAGNVYRTMAWPFGASPRKLAAHTNLAAVPFLAIFIYAQTGTIDLVPLAAIPSLVAV
                     QL"
BASE COUNT          343 a          693 c          527 g          421 t
ORIGIN      
        1 gcagctcatc gaaaatgccg ggcgcgccgt ggcggaggag atcacccggc gctttggtgc
       61 gcggagaacc gtagtcctct gcggccccgg caacaatggc gccgatggaa aggtggcggc
      121 gcgctatctc aagcaatggg gctggcccgt caccatcgcc gatggcattg agggcgcgga
      181 actcatcatc gatgcgctct atggcgctgg cctcaaccgc gactttcccg cagcgctggc
      241 cgataaaata aacactgccg gcgttcccat cgtttccgtt gacgtgccct caggacttga
      301 cggcctcact ggtcatccgc gtggctcttg cgtcaaggct gatctcacca ttacgttttt
      361 ccgcaagaag cccgcccacc tcctgctgcc cggccgcatg ctttgcggtg aagttgtcgt
      421 cgctgacatc ggcatccccg cgacggtttt gccggaactg aaaattcatc tctttgaaaa
      481 ctcaaaaccc ttccttcccg ctatcaattg cgcttcccac aaatacaagc gcggccatgc
      541 ggtcgttgtc tccggcgcaa agttcaacac gggtgcatcg cgccttgccg cacaggcagc
      601 tttaaaaatc ggggcagggc tcgtcaccat cgctgggcta caggatgaac tgcttgtcca
      661 tgctgcccac accaccgcaa tcatgctggc ccaaaccaat acggctctgg cactcggcat
      721 gttcctcaat gacgcgcgcc gcaacgccgt ctgcatcggc cccgccgcca gtgtaggctc
      781 cgaaacctgc gccagggtcc gcgccattct cgccagcggt gctgcgacag tgcttgatgc
      841 cgatgccttg acgtcctttg cccgaaaccc cgccgaactc ttcgccgcta ttgccgaacg
      901 tccccgccgg cccgtcatcc tcaccccgca cgaaggcgaa ttcgcccgcc ttttcaaaac
      961 cctggccacg gccccggaag caaaacatga gcgcgctctg aaggccgcaa aaagcgccgg
     1021 agccatcatc attctgaaag gcgctgatac cgtcatagca agccccgatg gccgcgccgc
     1081 gatcaactgc aatgccccgc cgtcacttgc caccgccggt tccggtgatg tgctggcggg
     1141 ccttgccact ggcttgctcg cccagggcat gcccgccttt gaggccgcct gcgccgccgt
     1201 ttggcttcat ggcgaggccg ccaataggca tggaccaaat ggactaacag cggagactct
     1261 tctcagtgca ctcatctaat gcctgccaga ttccggcgtt cgccggaatg acgagccaac
     1321 tgcaatgaac cgtccgcttt tccttcttct cggcaccggc gcggcccttg gattgaattt
     1381 ccccatcggt aagatggcca tggccgcagg cgtgacaccc gccctctggg ccgcggtgat
     1441 ttccgccggc gcagggcttg ccatgctgtt cattgtcgcg gtcaccgagc gcggagacct
     1501 gtcgcgcacc tccactttat ccttcgccat catctccggc ttcctctcct atgtcgtgcc
     1561 gaaccttctc acctattcag tgatcccgaa aatcggcagc ggccttgccg ccatcatgtt
     1621 cgcgctctcg ccggtggtga cagcgctgct ttccgtggtg ctccgggtgc gcccgccaaa
     1681 ccttctggga attttcggaa taacgcttgg attggccggc gccggcatca tcatattttc
     1741 gcggcagttg gatttcagcg gcagcacccc gtggattttg cttgccttgc ttatcccggt
     1801 ttttctcggc gcgggaaatg tctaccgcac catggcctgg cccttcggcg ccagtccccg
     1861 caagctcgcc gctcatacca atctcgccgc tgtccccttc ctagccatct tcatctatgc
     1921 ccagaccggc accatcgatc tggtacctct ggccgcaatt cctagcctgg ttgccgtgca
     1981 actc
//
LOCUS       DAORVN010000448         1982 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_62155_length_1982_cov_3.796575, whole genome shotgun sequence.
ACCESSION   DAORVN010000448 DAORVN010000000
VERSION     DAORVN010000448.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1982)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1982)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1982
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62155_length_1982_cov_3.796575"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..284)
                     /locus_tag="PKV23_08825"
     CDS             complement(<1..284)
                     /locus_tag="PKV23_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012110133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HQX85578.1"
                     /translation="MTPFGEKMRKLRADRAITLKQMSTAIGVSSAYLSALEHGKRGRP
                     GWHLIQRIIAYFNIIWDEAEEVTRLARISHPRITIDTSGLNPLATELANRL"
     gene            complement(286..855)
                     /locus_tag="PKV23_08830"
     CDS             complement(286..855)
                     /locus_tag="PKV23_08830"
                     /inference="COORDINATES: protein motif:HMM:NF013844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HQX85579.1"
                     /translation="MKKKSFEVHDRNLWDEVTRSVRPLGKRALRMSASLVSLPQQAAN
                     PVPQKKSTPKTSLNRAPPPITGFDRRTEQKLTRGNVEIESRLDLHGMNLEEARIRLLD
                     FISSRRMQGHRLVLVITGKGASPFSGHTLHGRDHFYAPEREGRLRRQLPEWLHEAQFR
                     AHVAGFQQAHPKHGGGGAFYVKLRRTRES"
     gene            complement(852..>1982)
                     /locus_tag="PKV23_08835"
     CDS             complement(852..>1982)
                     /locus_tag="PKV23_08835"
                     /inference="COORDINATES: protein motif:HMM:NF015523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MltA domain-containing protein"
                     /protein_id="HQX85580.1"
                     /translation="LFAIFLAMTAEAKPVFTAVPFAEIKGWADDDHAAALAAFQKSCA
                     EIKAKGHAFERQVRYGGKRADWLAVCESAAEAKSARQFFEENFTALKVADPASPEGLF
                     TGYYEPEAEGSLTPGNGYEVPIYRKPDDLVGFDAATEKRLGVKYGRMLNGKPAGYLTR
                     REIEKGALHGRGLEIVWLKSWVDAFFIHVQGSGRIRFEDGSSMRLAYGAKSGQPYTGI
                     GGVLVERGALTRDTMSMQSLRKWMKDNPGEARQLMWENKSFVFFREVKVDDPALGAPG
                     AQQVSLTPLRSLAIDRSLWVFGTPVWLDAEVPSGPGGGMENFRHLMVAQDTGTAIKGH
                     VRGDVYWGWGEKAASVAGQMKSPGTMIVLLPKKLAAGFLNGT"
BASE COUNT          362 a          589 c          635 g          396 t
ORIGIN      
        1 aggcggttgg cgagttcggt ggcgagcgga ttgaggcccg aggtatcgat ggtaatgcgc
       61 ggatgggaaa tgcgggccag ccgggtcacc tcctcggcct cgtcccagat gatgttgaaa
      121 taggcgatga tgcgctgaat caggtgccag cccggcctgc cgcgcttgcc atgctcgagg
      181 gccgagagat aggcgctgga aacgccgatg gcggtggaca tctgtttcag ggtgatggcg
      241 cggtcggccc gcagcttccg cattttttca ccaaagggcg tcatctcaac tctcccgggt
      301 gcggcgcagt ttcacataga aggccccgcc gccgccgtgc ttggggtggg cctgctggaa
      361 gccggcgaca tgggcacgga attgcgcttc gtgcaaccat tctggcaatt gccggcgcag
      421 gcggccctcc cgctcgggag cgtagaaatg atcgcggcca tgaagcgtgt gcccggaaaa
      481 cggcgaagcg cccttgccgg tgatgaccag cacgagacgg tggccctgca tgcggcggct
      541 ggaaataaaa tccagaagac ggatgcgggc ttcctcgaga ttcatgccat gcaaatcaag
      601 ccggctttca atctcgacat tgccccgggt cagcttctgt tcggtgcgcc ggtcaaagcc
      661 ggtaatcggc ggcggcgccc tgttgaggga agtctttggc gtcgatttct tctgcggcac
      721 aggattagcg gcttgctgcg gcagcgaaac caatgaggcg ctcattctta gcgcgcgctt
      781 gcccaggggc ctgacggagc gggtgacctc atcccagaga ttgcggtcgt gaacctcgaa
      841 ggatttcttt ttcatgtccc gttcaagaat ccggccgcaa gtttcttggg cagcaggacg
      901 atcatggtgc cggggctttt catctggcca gccacgcttg cggctttctc gccccagccc
      961 caatagacat cgccgcgcac atggcccttg atggccgtac cggtatcttg cgccaccatc
     1021 aggtgacgga agttttccat cccgccgccg gggccggacg gcacttccgc atcaagccag
     1081 acaggcgtgc cgaacaccca gaggctgcgg tcgatggcga ggcttcgcag cggcgtgagc
     1141 gagacctgct gggcgccggg ggcgccaagc gcggggtcgt cgaccttcac ctcgcggaag
     1201 aaaacgaagg acttgttttc ccacatgagt tgacgcgcct cgccgggatt gtccttcatc
     1261 catttgcgca gggattgcat cgacatcgtg tcccgggtga gcgcgccgcg ctccaccagt
     1321 acgccgccga tgccggtata gggctggccg gatttggcgc cataggcaag ccgcatgctg
     1381 ctgccatctt caaagcggat gcggcccgag ccctggacat ggatgaagaa cgcgtcgacc
     1441 cagctcttga gccagacgat ttctaggccc cggccgtgca gggcgccttt ctcgatttcg
     1501 cggcgcgtca aatatccggc gggcttgccg ttcagcatgc ggccgtattt cacgccgagc
     1561 cgcttttcag ttgccgcatc gaaaccaacg aggtcatcgg gcttgcggta gatgggaact
     1621 tcatagccat tgccgggtgt aaggctgcct tccgcttccg gttcgtaata gccggtgaac
     1681 aggccctcag gactggcggg atcagcgacc ttcagggcgg tgaaattttc ctcgaaaaat
     1741 tggcgcgccg attttgcctc tgcggcgctt tcgcaaaccg caagccagtc cgcacgtttc
     1801 ccgccatagc gaacctggcg ttcaaaggcg tggccctttg cctttatctc tgcgcaggat
     1861 ttttgaaagg ccgcaagggc tgccgcatgg tcatcatcgg cccagccctt gatctcggca
     1921 aagggcacgg cagtgaaaac gggtttggct tcagcggtca tggcaaggaa aatggcgaaa
     1981 ag
//
LOCUS       DAORVN010000449         1981 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_62224_length_1981_cov_2.936656, whole genome shotgun sequence.
ACCESSION   DAORVN010000449 DAORVN010000000
VERSION     DAORVN010000449.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1981)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1981)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1981
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62224_length_1981_cov_2.936656"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="PKV23_08840"
     CDS             complement(<1..186)
                     /locus_tag="PKV23_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002965910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HQX85581.1"
                     /translation="MANGMLTLEELKAEVKAGRIDTVVAAFPDMLGRLMGKRFEANFF
                     VDGAYEETHGCNYLLAVD"
     gene            complement(197..946)
                     /locus_tag="PKV23_08845"
     CDS             complement(197..946)
                     /locus_tag="PKV23_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007199219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HQX85582.1"
                     /translation="MTKNLLEGEEDAFILVNEAGSSPYVLVCEHASNRLPKQLGNLGL
                     SADDLQKHIAWDIGAEGVSRLLSRLLDAPLVLQRYSRLAYDCNRPPDSPGAMPEMSET
                     TPIPGNMGLSARDKMARVSAIYWPFHERLARMLDQRACEGRATSLVTIHSFTKMYKGK
                     EREVELGILFDRDARIADRLIKAFPKVDVRLNEPYGPKDGVLHTLNLHAAPRGLKSAM
                     IEIRNDFIANERGQNEWAQRLAMPLIHATTK"
     gene            1060..>1981
                     /locus_tag="PKV23_08850"
     CDS             1060..>1981
                     /locus_tag="PKV23_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019963327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HQX85583.1"
                     /translation="MQEFDFIIIGSGSAGSAMAYRLSEDGKNTVLVLEFGGSDRGPFI
                     QMPSALSYPMNMNTYNWGFETEPEPHLGGRRLATPRGKVMGGSSSINGMVYVRGHARD
                     FDAWEEMGAKGWGHRHVLPYFKRLENSHGGEEGWRGTNGPMHVKRGRRLNPLYQAFVD
                     AGREAGYPVTPDYNGYQQEGFGPMEMTVHHGFRWSAANAYLRPAMRRPNVKLVTRAYV
                     HRVVLEGKRATGVEYEVGGEVVTARARREIVIAASSINSPKILQLSGIGAPEVLKAAG
                     LPVNHVLPGVGENLQDHLEIYFQMESKRPVT"
BASE COUNT          375 a          625 c          583 g          398 t
ORIGIN      
        1 atcgaccgcc agcagatagt tgcagccgtg ggtttcttcg taagcgccat ccacaaagaa
       61 attcgcctca aatcgcttgc ccatgagtcg gcccagcatg tcgggaaaag cggcaacgac
      121 cgtatcaatc ctgcctgctt taacctccgc cttcagctct tccagtgtca gcatgccatt
      181 ggccatctaa gtctccctat tttgttgtcg catgaatgag cggcatcgcc aggcgctgcg
      241 cccattcatt ctgtccgcgc tcgtttgcaa tgaagtcatt gcggatttcg atcattgcgg
      301 atttcagccc ccgcggcgcc gcatgcaggt tgagagtgtg cagcactcca tccttcgggc
      361 catagggttc atttagccgc acgtccactt tcggaaaggc cttgatcagc cggtcggcga
      421 tgcgcgcgtc ccggtcaaac aggatgccga gttcgacctc ccgctccttg cccttgtaca
      481 ttttcgtgaa gctgtgaatg gtcaccaggc tggttgcgcg cccctcgcag gcgcgctggt
      541 ccagcatgcg ggccagccgc tcgtgaaacg gccagtaaat ggccgaaacg cgggccatct
      601 tgtccctggc actcaggccc atgtttccgg gaattggcgt cgtttcactc atttccggca
      661 tggcgccggg cgagtcaggc ggccggttgc agtcataggc caggcgcgaa tagcgctgca
      721 gaaccagggg cgcatccaga agccgcgaaa ggagccgcga aaccccctct gcgccgatgt
      781 cccaggcgat atgcttttgt aggtcatcgg ccgaaaggcc gagatttccg agctgtttgg
      841 gcaggcggtt gctggcatgc tcgcacacca ggacataggg cgaagaaccc gcctcattca
      901 ccagaatgaa cgcgtcctct tccccttcaa gcagattttt ggtcatttcc ggcagatttc
      961 ccttgccgcg cgttgttcca gaactgtcgc tgcctgtcaa acactggcaa agtataaagt
     1021 gcacatggaa ggcatctgcc gggggatggg ctgggaaata tgcaggaatt cgactttatc
     1081 atcataggct ccggctccgc cggatcggcc atggcctatc gcctctcgga ggacggcaag
     1141 aacaccgttc ttgtgctcga attcggcggc agcgaccgcg gccccttcat ccagatgccc
     1201 tcggccctgt cctaccccat gaacatgaac acctataact ggggctttga aaccgagccc
     1261 gaaccccatt tgggcgggcg caggctggcc acgccccgcg gcaaggtcat gggtggctcg
     1321 tcctcgatca acggcatggt ttatgtcagg ggccatgccc gggatttcga tgcctgggag
     1381 gaaatggggg ccaagggctg gggccaccgc catgtgctgc cctatttcaa gcgcctggaa
     1441 aactcccatg gcggcgagga aggctggcgg ggcaccaatg gccccatgca tgtgaaacgc
     1501 ggccgccggc tgaatccgct ctaccaggcc tttgtcgatg ccggccgcga ggcgggctac
     1561 cccgtgaccc cggattacaa cggttaccag caggagggct tcggccccat ggaaatgacc
     1621 gtgcaccatg gctttcgctg gtccgccgcc aacgcctatc tccggccggc gatgcgccgc
     1681 ccgaatgtca agctcgtgac ccgcgcctat gttcaccgcg tcgtcctcga gggcaagcgc
     1741 gccaccgggg ttgaatatga ggtaggtggt gaagtggtga ccgccagggc gcggcgcgag
     1801 attgtcatcg cagcctccag catcaactcg ccgaaaatcc tccagctctc cggcatcggc
     1861 gcgcccgagg tgctcaaggc cgctggcctg ccggtaaacc atgtgctgcc cggcgtcggc
     1921 gagaacctgc aggaccatct ggaaatctat ttccagatgg aatccaagcg gcctgtcacc
     1981 c
//
LOCUS       DAORVN010000450         1980 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_62264_length_1980_cov_4.020779, whole genome shotgun sequence.
ACCESSION   DAORVN010000450 DAORVN010000000
VERSION     DAORVN010000450.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1980)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1980)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1980
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62264_length_1980_cov_4.020779"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1830
                     /gene="glnD"
                     /locus_tag="PKV23_08855"
     CDS             <1..1830
                     /gene="glnD"
                     /locus_tag="PKV23_08855"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: protein motif:HMM:TIGR01693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0018177 - protein uridylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HQX85584.1"
                     /translation="GYQSHAGLKNVERFMKHYFLIAKEVGDLTRIFCASLEAKQVKDI
                     PVLSRVMGRFRSKPVVKIPENKNFQIESGRLALVKPSVFKEDPVNLLRLFSVASIHGA
                     EIHPDALKTVRKSLSLIDKELCNNAEANALFLDMLVNSDSSETLLRMMNEAGVLGRFI
                     PEFGKVVAMTQFNMYHHYTVDEHLIRAVGILAALEKGRLSEEHPLSVVLFPTLKSRRS
                     LYVATLLHDIAKGRTEDHSIAGERIARALGPRLGLSPEETETTAWLVRHHLLMSETAQ
                     MRDLNDFKTILDFTAVVQSPERLKLLLILTVVDIRAVGPGVWNGWKGQLLRTLYAEAE
                     PVLSGGHTGSSREVRVKEAQAAFLAHFPQWSDSQIRTATSRHYDAYWLNVSLEKQIAH
                     QALIDRSSPRDIVTEVHTDAFTAITELTIYAPDHPRLLALITGACAAANANILGAQIF
                     TTTDGMALDTILIQREFSTEDDERRRAERVTEIIRQALEGKLRLRDAVAGATQQRSRL
                     KAFSVAPRVIIDNQSSNRFTVIEINGLDRIGLLYKLTEALFHLSLNIASAHITTFGEK
                     AIDVFYVTDLTGDKILSPARHAQIERELMKVLAPMPERLANSA"
BASE COUNT          426 a          592 c          579 g          383 t
ORIGIN      
        1 ggctaccagt cccatgccgg attgaaaaat gtcgagcgtt tcatgaagca ttattttctg
       61 atcgccaagg aagtgggcga tctcacgcgg attttctgcg ccagcctgga agcaaagcag
      121 gtgaaggata tacccgtctt gagccgcgtc atggggcggt tccggtccaa gcccgtcgtc
      181 aagataccgg aaaacaagaa tttccagatt gagagcggcc gcctggcgct ggtcaaacca
      241 agcgtattca aggaagatcc ggtcaacctc ctgcgcctgt tttctgtggc cagcatccat
      301 ggcgcggaaa tccacccgga tgcgctcaag acggtcagaa aatcgctttc cctgatcgac
      361 aaggaactat gcaacaatgc ggaagccaat gccttgtttc tggacatgct ggtcaacagc
      421 gactcatcgg aaaccctgct gcgcatgatg aatgaggcgg gggtcctggg ccgcttcatt
      481 ccggagttcg gcaaggtcgt agcgatgacg cagttcaaca tgtatcacca ctatacggtg
      541 gacgagcatc tcatccgtgc cgtcggcatc ctcgcggccc ttgaaaaagg acggctgtcg
      601 gaagaacatc cgctctcggt ggtgctcttt cctaccctca agagccgccg ctcgctctat
      661 gtcgcaacgc tcctgcacga tatcgccaag ggccggacgg aagaccattc cattgccggt
      721 gaacggattg cgcgcgcctt gggcccgcgc ctaggccttt cgccggaaga aaccgaaaca
      781 acggcctggc tggtgcgcca ccacctgctg atgagcgaga cggcgcagat gcgcgatctc
      841 aatgacttca agaccattct ggattttacc gcggtcgtgc aaagcccgga gcggctgaag
      901 ctgctgctga tcctgacggt ggttgacatc cgcgccgtgg ggcccggcgt gtggaacggc
      961 tggaaaggcc agctcttgcg cactctctat gcggaagcgg agccggtgct gtcggggggt
     1021 catacgggaa gcagccgcga ggttcgtgtc aaggaggcgc aggccgcttt tctggcgcat
     1081 tttccgcagt ggtcggacag ccagatcagg acggccactt cgcgccatta cgatgcctac
     1141 tggctgaatg tcagcctcga gaagcagatc gctcaccagg cgctgatcga ccggtcgtcg
     1201 ccccgcgaca tcgtgacgga agttcatacg gacgccttca cggcaatcac ggaactcacc
     1261 atctacgcgc cggaccatcc gcgcctgctg gccctgatca cgggcgcctg tgccgcggcg
     1321 aacgccaaca tcctgggagc gcaaatcttc acgacgacgg atggcatggc actggacaca
     1381 atcctcatcc agagggaatt ctccaccgag gatgacgaaa ggcgccgggc cgagcgggtg
     1441 accgaaatca tccgccaggc cctggaggga aagctgcggc tcagggatgc tgttgccggc
     1501 gccacacagc agcggtcacg gctcaaggca ttttcggtgg cgccccgcgt catcatcgac
     1561 aatcagtcgt caaaccgttt cacggtgatc gaaatcaacg ggcttgaccg cataggcctg
     1621 ctttacaagc ttaccgaagc gctgtttcac ctcagcctga acatcgcttc ggcccacatc
     1681 accacgtttg gcgaaaaggc catcgacgtt ttctatgtca ctgatctcac cggcgacaag
     1741 attctcagcc cggcgcggca cgcacaaatt gaacgggaac tgatgaaggt cctggccccc
     1801 atgccggaaa gacttgcaaa ctccgcatga atcagtggaa ggaagggcct gaacttggat
     1861 attgagatat gtcgctcctg aaatcggcag caaccgtcgg cgggtttacc ctgctcagcc
     1921 ggctgcttgg attcttgcgt gaccagctta ttgcctacac gctgggaacc gggaccgtcg
//
LOCUS       DAORVN010000451         1975 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_62538_length_1975_cov_3.521354, whole genome shotgun sequence.
ACCESSION   DAORVN010000451 DAORVN010000000
VERSION     DAORVN010000451.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1975)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1975)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1975
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62538_length_1975_cov_3.521354"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..655)
                     /locus_tag="PKV23_08860"
     CDS             complement(<1..655)
                     /locus_tag="PKV23_08860"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQX85585.1"
                     /translation="MITKPRINPWIIVWLCFATLSIVMSARQSVSVMTDEWANTLGWS
                     KTFIGSGQSVALIMVAIFSPVAGNLADRFGARIMLVAGLLLVGTGLLAFAVVPVAAIF
                     IIGYGLIAGVGFATANMHLISTALAKLFKENRGLATGIANSGATAGQFITVPLLAIGL
                     TLFSWRWSIIAAAAACALMALVIWVMLKPTEEESAAQKSETISDEPLWARLKFLLTNP
                     "
     gene            790..1647
                     /gene="dapF"
                     /locus_tag="PKV23_08865"
     CDS             790..1647
                     /gene="dapF"
                     /locus_tag="PKV23_08865"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012110311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HQX85586.1"
                     /translation="MTQTGALPFRKMNGLGNDFVVLDGRVRAISMDDRRAQAIADRKT
                     GIGCDQIIILEKSGIADVRMLVWNSGGGQVESCGNAARCIADMLFDENKATSATIDTL
                     GGFLVARKAGEKMVTIDNGVPRFDWKDIPLSEPFHDTRHIELQVGPIDHPLIHSPSVV
                     NVGNPHCIFWVDDLDVVDLSKVGPMLEHHHLFPERANISLARVDARDHVTLKVWERSA
                     GLTKACGTAACATMAAGARIKIIDRKCRITLPGGDLMMEWRASDDHVLMTGPVAYEFD
                     GVLPQELAA"
     gene            1644..>1975
                     /locus_tag="PKV23_08870"
     CDS             1644..>1975
                     /locus_tag="PKV23_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019566026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HQX85587.1"
                     /translation="MSESDFVTFGCRLNTYESEVMRGHAAKAGLKDAIVFNTCAVTAE
                     ATRQARQAIRKAHKDRPDAKIIVTGCAAQTTPEMFAEMPEVSMVLGNEEKLQPQNWLP
                     GANDRVRV"
BASE COUNT          406 a          542 c          672 g          355 t
ORIGIN      
        1 taggattggt gagcaggaac ttgaggcggg cccacagggg ctcgtcggaa atggtttcgg
       61 atttctgcgc cgctgattct tcctcggtgg gcttcagcat gacccagatg acaagggcca
      121 tcagggcgca agcggcggcg gctgcgatga tgctccagcg ccaggaaaac aaggtaaggc
      181 cgatggccag cagcggcacg gtgatgaact ggcccgcggt tgcgccggaa ttggcgatgc
      241 cggtggcaag cccgcggttt tccttgaaga gtttggccag ggccgtggag atgagatgca
      301 tgttggcggt ggcaaagccc accccggcga taagcccgta accaatgatg aaaatggcgg
      361 cgacgggcac cacggcgaaa gccagcagtc cggttccaac cagcaggagg cctgccacca
      421 gcatgatgcg ggcgccgaag cggtccgcca ggttgccggc cacgggggag aagatggcca
      481 ccatgattag cgccacggac tggcctgagc cgatgaaggt ttttgaccag cccagggtgt
      541 tggcccattc gtcggtcatg acggaaacgg actggcgggc ggacatgacg atggaaaggg
      601 tggcaaaaca cagccacacg atgatccagg gatttatgcg gggtttggtg atcatggctc
      661 atgttgagca tggcgcgggg gccttgggct agtacgataa ttgtaccggc aggcccgcgg
      721 ccccgattcc aagggggtac ggagttgcgg ctggcttttt gggccggttt caccatataa
      781 ccccctgaaa tgacccagac aggcgcactt ccctttcgca agatgaacgg gctcggcaat
      841 gattttgtcg tgcttgacgg gagggtgcgc gccatttcca tggatgaccg gcgggcccag
      901 gccatcgccg accggaaaac cggcatcggc tgcgaccaga tcatcatcct ggagaaatcc
      961 ggtattgccg acgtgcgcat gctggtgtgg aacagcggcg gcggccaggt tgaaagctgc
     1021 ggcaacgcgg cgcgctgcat tgccgacatg ctcttcgacg agaacaaggc gacgtccgcc
     1081 accatcgaca cgttgggtgg ctttctggtg gcgcgcaaag caggcgagaa gatggtgacc
     1141 attgacaatg gcgtgccgcg ctttgactgg aaggacattc cgctgtccga gcctttccat
     1201 gacacgcgcc acatcgaact gcaggtcggc cccatcgatc acccgctcat tcattcgccc
     1261 tcggtggtga atgtgggcaa tccgcattgc atcttctggg tcgatgacct cgatgtggtc
     1321 gatctttcaa aagttggtcc catgctggaa catcatcacc tgtttccgga gcgggcgaat
     1381 atctcgctgg cgcgcgtcga tgccagggat catgtgacgc tgaaggtgtg ggagcgcagc
     1441 gctggcctca ccaaggcctg cggcacggca gcctgcgcca cgatggcggc gggtgctcgg
     1501 atcaagatca ttgaccggaa atgccgcatc accctgcccg gcggggatct gatgatggaa
     1561 tggcgcgcaa gcgacgacca tgtgctgatg accgggccgg tggcttatga atttgacggc
     1621 gtgctgccgc aagagcttgc cgcatgagcg aaagcgattt cgtcaccttc ggctgcaggc
     1681 tcaataccta cgagtcggaa gtgatgcgcg gccatgcggc gaaggccgga ttgaaggacg
     1741 cgattgtctt caacacctgc gcggtgacgg cggaagcgac gcggcaggcg cggcaggcca
     1801 tccgcaaggc gcacaaggac aggccggacg cgaagatcat tgtcacaggc tgtgcggcgc
     1861 agacaacgcc ggagatgttt gccgagatgc cagaggtttc aatggtgctt ggcaatgaag
     1921 agaaactgca gccgcagaac tggctgcccg gggccaatga ccgggtgcgg gtaag
//
LOCUS       DAORVN010000452         1971 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_62760_length_1971_cov_2.725470, whole genome shotgun sequence.
ACCESSION   DAORVN010000452 DAORVN010000000
VERSION     DAORVN010000452.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1971)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1971)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1971
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62760_length_1971_cov_2.725470"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..744)
                     /locus_tag="PKV23_08875"
     CDS             complement(<1..744)
                     /locus_tag="PKV23_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018236618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HQX85588.1"
                     /translation="MSDQASKALAFRALHTRSGAFLIPNPWDAGTAKILTHLGFEALA
                     TTSAGLAFALGRNDGAASREETLANARAIVQATHLPVSADLENGFGHSPDKVAETIQL
                     AASAGLVGGSIEDASGDRDKPIYDLNLAAERISAAVEAACAQNIHFTLTARAENFLHG
                     RPDLDDTIERLQAFEKAGADVLYAPGLPSLDAIREVCSAVSRPVNVVAGLSGPVFSLK
                     QLEDAGVKRVSVGSSLSRLALAAVAGAARE"
     gene            870..1574
                     /locus_tag="PKV23_08880"
     CDS             870..1574
                     /locus_tag="PKV23_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015930707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HQX85589.1"
                     /translation="MAHIVSIAEIAALVGDPTRANMLEAMLDRRALTARELALRAGIA
                     PQTASGHLSKMITAGLIAMERHGRHHYHSLASSEIARMLEGLQQVASTQNIKHAGRTM
                     RTGPRDAAMRVARGCYDHMAGHLAVSVSDAMLGRGQIEFEGDGGRVTESGKLFLDRFG
                     IDLAGTRHSKRVFCRPCLDWSERRYHLAGAVGAALLHRTLELKWVKRQDNTRALAITR
                     AGQEGFRKTFGIELAI"
     gene            1726..>1971
                     /locus_tag="PKV23_08885"
     CDS             1726..>1971
                     /locus_tag="PKV23_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012563134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HQX85590.1"
                     /translation="MIFLGLSGALAYVIWDVSKGLPDYKQLASYEPPVMSRIHAADGS
                     LLAEYANERRLFIPINSVPKRLIQAYISAEDKTFFTHG"
BASE COUNT          411 a          578 c          575 g          407 t
ORIGIN      
        1 ctcgcgggcg gcccctgcga cagcggccag tgccagccgg ctcagggagg aaccgacact
       61 gacacgtttc acccccgcat cttccaattg cttgagcgaa aacactggcc cgctcaatcc
      121 agccaccaca ttcaccgggc ggctgactgc tgaacagact tcgcgaattg cgtcaaggct
      181 tggcaggccc ggcgcataaa gcacgtcggc cccggccttt tcgaaagcct gcaggcgttc
      241 gattgtgtca tcaaggtcag gccgtccatg caggaaattc tcagcccgcg ccgtcagcgt
      301 gaagtgaatg ttctgcgcac aggcagcttc caccgccgct gaaatccgtt ctgccgccag
      361 attgagatca tagattggct tgtctcggtc tccgctcgcg tcctcgattg acccgccaac
      421 aagtccggcg gaagcagcaa gctgaattgt ttcggcaact ttgtcagggg aatgcccaaa
      481 tccgttttcc agatcagctg aaactggaag atgagtagcc tgcacaatgg cgcgggcgtt
      541 cgccagggtt tcttcgcggc tggcggctcc atcattgcgg cccagggcga atgccagccc
      601 tgcactggtc gttgccagtg cttcaaatcc caaatgcgtc aggattttag cggtgcccgc
      661 atcccaggga ttgggaatga gaaaggcacc ggagcgggtg tgaagggcgc ggaatgcaag
      721 ggctttgctg gcttggtcgg acatggggca ggctcctcaa aatgttgaag tgttctattc
      781 ccggcgaacc tcttaatgct acggtgatga ccgtagcatg cacgatggat gaagtgttac
      841 ggctgtagaa atctggtgga gaaagcccca tggctcatat tgtcagcata gcggaaattg
      901 ctgccctggt cggggatccg acccgggcaa atatgctgga agcgatgctg gaccgccggg
      961 cgctcaccgc aagggaactg gcgttgcgcg ccggcatcgc gccgcaaact gccagcggac
     1021 atctttccaa aatgatcacc gcgggactca tcgcaatgga acggcatggc cgccatcact
     1081 accactcatt ggcctcatcc gagatcgcac gcatgctcga aggcctgcag caggtggctt
     1141 caacccagaa catcaagcac gccgggcgca ccatgcgcac cggtccgcgc gatgccgcca
     1201 tgcgtgtcgc ccgcggatgc tacgatcaca tggccggcca cctcgcggtg agcgtcagcg
     1261 atgcaatgct tggccggggg cagattgaat tcgaaggcga tggcggcaga gtaactgaat
     1321 ccggcaaatt gtttctcgac aggttcggca tcgatcttgc cggcacaaga cactcgaagc
     1381 gcgtcttctg caggccttgc ctcgactgga gcgaaagacg ctatcatctc gcaggtgctg
     1441 tgggcgctgc cctgctgcac cggaccctgg agctgaaatg ggtgaaacgc caggacaaca
     1501 ccagggcgct ggccattacc cgggccgggc aagaaggctt ccgcaagact tttggcatag
     1561 aactggccat ttagcaggtg ctttgcgcct gcctatcctt ggccatattc caagcctagc
     1621 aagggccaca tggcatgtgc tatcgccagc aggagatagt gacggaatcg gatcaataat
     1681 tctgcgatgt taagattttt aggatggctc ttcggagctg ggttcatgat tttcctgggc
     1741 ctctcggggg ctctggctta tgtgatttgg gatgtgtcca aaggccttcc ggattacaag
     1801 caactggcct cctatgagcc ccccgtcatg tcgcgcatcc atgccgccga tggttcgctg
     1861 cttgccgaat atgcaaatga gcggcgcctg ttcatcccta tcaattccgt ccccaagagg
     1921 ctgatccagg cctacatttc tgcggaagac aaaacctttt tcactcacgg c
//
LOCUS       DAORVN010000453         1970 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_62816_length_1970_cov_3.042820, whole genome shotgun sequence.
ACCESSION   DAORVN010000453 DAORVN010000000
VERSION     DAORVN010000453.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1970)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1970)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1970
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62816_length_1970_cov_3.042820"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..332)
                     /locus_tag="PKV23_08890"
     CDS             complement(<1..332)
                     /locus_tag="PKV23_08890"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HQX85591.1"
                     /translation="MIFKRSKPSTNGTAAPSNSGPSIVAQDVTIEGNITAAGELHIDG
                     TVFGAVRAKSCVVDMNGFVQGELVAEEIFIRGRVMGPIRGLHVNLYAGAQVEGDILNE
                     TISIENGA"
     gene            complement(301..1569)
                     /locus_tag="PKV23_08895"
     CDS             complement(301..1569)
                     /locus_tag="PKV23_08895"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HQX85592.1"
                     /translation="MAKRHQIKNEWLMKRRSPLRGFLQTRQIYVRSNGDVQFITVSPI
                     SQVCLLIIFLIGFLWMAFASVNIVFKDQLLELKQQKLFEARLDYENRLSELRASIERV
                     NDRLLLDQQGYLKKVDEVKAEFSSLAEQQKTMENFFKEGWFPLKESAAPEKFQVDGAL
                     EGTFAQRYASDFRSRLEAVKPLDEMNASFTGLHKQQLALLKDAQAYSQRKLQTTSVLL
                     SRLGIRPPVTVNLVKGDAAAMGMGGPFLSVPKRTQREYEIADGIETVHATLEQETKVR
                     QAVADLPLVKPMTQITGISSSFGYRKDPLRLSLAFHGGIDFKGIYAAPVLATSNGQVT
                     WAGPHGPYGNLVEIFHDNGVSTRYGHLKSVNVSLGQKISRGDLVGWMGSTGRSTGPHL
                     HYETRVNGRVIDPQDFWRTQHDLQALETLN"
BASE COUNT          423 a          554 c          523 g          470 t
ORIGIN      
        1 ttggcgccat tctcgatgga aatcgtctcg ttaagaatat cgccttccac ctgcgcgccc
       61 gcatacagat tgacatgaag accgcggatc ggccccatca cccggccgcg aatgaaaatc
      121 tcttccgcca ccagctcacc ctggacaaag ccgttcatgt cgaccacgca ggattttgcc
      181 cgcaccgcac caaaaaccgt gccgtcaata tgaagctctc cggccgccgt gatattgccc
      241 tcgatggtca catcctgggc aacgatcgac ggccccgaat tcgaaggggc ggcggttccg
      301 ttagttgagg gtttcgagcg cttgaagatc atgttgtgtt cgccaaaaat cttgaggatc
      361 aatcacacgg ccatttacac gggtttcgta gtgcaagtga ggccctgtgc tccgccccgt
      421 gcttcccatc cagccaacca agtcgccacg gctgattttt tggcccagcg aaacgttaac
      481 ggacttgaga tgaccatacc gggttgagac gccgttgtca tgaaagattt caacaaggtt
      541 accatagggc ccgtgcggcc cggcccatgt tacctggccg ttcgatgtgg caaggaccgg
      601 cgcggcatag atacccttga aatcaatgcc gccatgaaac gccagcgaca gccgcaacgg
      661 gtctttgcgg taaccaaagg aactggaaat cccggtaatc tgcgtcatcg gcttgacgag
      721 cggcaaatcc gcgaccgcct ggcggacctt ggtctcctgt tccagcgtgg catgcacggt
      781 ctcaatgccg tcagcaattt catattcacg ctgagttcgc ttgggcacgc tgagaaatgg
      841 ccctcccatc cccatggccg cagcatcacc tttaacaaga ttaacggtga cgggaggccg
      901 gatgcccaat ctgctgagga gcacgctcgt cgtctgtagt ttgcgctggg aataggcctg
      961 agcatctttc agcagtgcca attgctgttt gtggaggcca gtgaaactgg cgttcatttc
     1021 atcgagtggc ttgacggctt ccaggcgtga ccggaagtca gacgcatagc gttgcgcgaa
     1081 ggtgccttcc agtgcgccat ccacttgaaa tttttcgggc gcggcgcttt ccttgagcgg
     1141 aaaccagccc tctttgaaaa agttctccat ggttttctgc tgctccgcca ggctgctgaa
     1201 ttcggctttc acctcatcca ctttcttcag atagccttgt tggtccagca gcaaccggtc
     1261 gttgacccgc tcaatcgagg cccggagctc tgacaggcgg ttttcatagt ccagccgggc
     1321 ctcgaacagc ttctgctgct tgagttccag caactggtcc ttgaagacga tgttgacgct
     1381 ggcgaaggcc atccacagaa agccaatcag gaagatgatg agcaggcaaa cctgggaaat
     1441 tggcgaaacc gtgatgaact gcacatcgcc gttgctgcgc acgtagatct ggcgtgtctg
     1501 caagaagcca cgcagcggcg aacgccgctt cattaaccac tcgtttttga tctgatggcg
     1561 tttggccatc aacctggtcc cagccatgaa cctgcctagg attttgcctc gatttctcaa
     1621 atttgccagt tttgcgcctg aacgcaagtc ctatgtacag gcctgggaag tgtgggcaaa
     1681 ttgccctcaa atcctgccat tgtttagcct agctttactg gccaatggcg ttagaattgt
     1741 gccgcaggta ttataatgct ggttctaatt ggctgattct gtttggaaat gatgacattt
     1801 tagagcgata acttagggca gttaagaatt gcgccatcgg ctggctaaaa taaccgtact
     1861 tgtcttgcta tcgattaccg caattctggt catcgtcggg gcagcctttt tctggcgcct
     1921 gtcgcaaggc ccggtttccc tggatttcat gaccgaacgg atcgaacggg
//
LOCUS       DAORVN010000454         1968 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_62919_length_1968_cov_3.406168, whole genome shotgun sequence.
ACCESSION   DAORVN010000454 DAORVN010000000
VERSION     DAORVN010000454.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1968)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1968)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1968
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62919_length_1968_cov_3.406168"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(70..909)
                     /locus_tag="PKV23_08900"
     CDS             complement(70..909)
                     /locus_tag="PKV23_08900"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HQX85593.1"
                     /translation="MLFPIFYGIIAPDIATPKSLGAMKTAIGLAAKLDGQLSVAIGAL
                     EISVRNTLSSGIVDRVVAEEGQRSRESAKALSGQIADLARGSGSAVHTEILSGSLGQI
                     TGQCAVRARIHGLVFAEAGGPSEPLTGALIEPLLFESGRPVIVVPNGFSSEISLSHIL
                     IAWDGSEGAARAVWDAIPLLLQANTLEIATVVGEKELDEVPAANALAGMLTFLGKKIT
                     VTPLTFDGNTAAGLIKQHAARTSAGLVVQGAYGRSRWSELILGGVTREMLHDCALPVL
                     MSH"
     gene            1060..1287
                     /locus_tag="PKV23_08905"
     CDS             1060..1287
                     /locus_tag="PKV23_08905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85594.1"
                     /translation="MRTAFAVLSLALATSLALADDSLWSSHDYVDFYFRHYNGHVPLP
                     HLRDETQKDLFRHLTDPGKITRIQRALSSAP"
     gene            complement(1250..>1968)
                     /locus_tag="PKV23_08910"
     CDS             complement(1250..>1968)
                     /locus_tag="PKV23_08910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85595.1"
                     /translation="WRQALMVLAAIQIPFGILIHWFVLGSSNERVVVSKANVPKGRVR
                     RAMATPVFWLLALSYGAHAFMFTGLTFHIIPLLTERGFALPMIIAAYTLVGPFQVIGR
                     IVVFALEKRIDVRLAGMAGTSLPVIGLVLLMLATPGSPLLYWFAIFYGGGMGIKTIVQ
                     ATAGPEFLGREGYGALQGTFAGINFAVQAATPFALAVLWSLMGGYDQVIWVLFAGAAL
                     SALAFVGALMVRRKGPAECG"
BASE COUNT          425 a          635 c          551 g          357 t
ORIGIN      
        1 gccggcacgc tcgacagcct gctcgacagt ggcggcgccg aagccaagtc agatcctccg
       61 ggttactctc taatgggaca tcaaaaccgg cagcgcgcag tcatgaagca tttcgcgcgt
      121 cacgccgccg aggataagtt cactccaccg cgagcggccg taagccccct ggacgaccag
      181 acccgcgctg gtcctggccg cgtgctgctt gatgaggcca gctgcggtgt tgccgtcaaa
      241 ggtgagcggc gtaacggtta tcttcttccc caggaatgtg agcatcccgg caagggcgtt
      301 cgcggcggga acttcgtcca gttccttctc gccgacaaca gttgcgattt ccagcgtgtt
      361 tgcctggagc agcagcggaa ttgcgtccca gacggcgcgc gccgcacctt cgctgccatc
      421 ccacgcgatc agaatatggc tcaacgaaat ctcggaggaa aacccgttag ggacgacaat
      481 gaccggcctg ccactttcaa aaagaagggg ctcgatcagc gctcccgtaa gcggctcgct
      541 gggaccgccc gcttcggcaa agaccaggcc atggattctg gcccgtaccg cacactggcc
      601 tgtgatctgg ccgagactgc cgctcaagat ctcggtgtgg actgctgaac cggaaccacg
      661 ggcaagatcg gcgatctgtc cactgagcgc ctttgctgat tccctgctgc gttgaccttc
      721 ctcggctaca acgcggtcca cgattcctga gctgagggtg tttcgaaccg aaatctccaa
      781 tgcgccaata gcgaccgaga gctgcccgtc aagttttgca gccagtccaa tagccgtctt
      841 catggcaccc agtgactttg gagttgcgat atcgggagcg atgatgccgt agaagattgg
      901 aaatagcatg cttgtggttc ctcttgtata cttgccaaca cctacaatga cctgttggag
      961 gttatattga catgaatcaa ccggagcaat gaaactccgc cggaccagct ccagcaaaat
     1021 cacttctgcg ccacttgatt tccatatgag tgctgggaca tgcgaactgc ttttgccgtc
     1081 ctgagtctgg cccttgcaac gagcctcgcc ttggcagacg acagcttatg gtccagccac
     1141 gactatgtgg atttctattt ccgccactac aacggccacg tgccgctgcc gcatttgcgc
     1201 gacgaaacgc agaaggacct gttcaggcat cttaccgatc ccggcaaaat cacccgcatt
     1261 cagcgggccc tttcctccgc accatgagcg ccccgacaaa ggccagcgcc gacagcgccg
     1321 ccccggcgaa cagaacccag atcacctggt catagccgcc catgagcgac cacagcaccg
     1381 cgagcgcgaa cggcgttgcc gcctgaaccg cgaaattgat tccggcgaat gtgccctgga
     1441 gcgcgccata gccttcccgc cccaggaact ccggccccgc cgttgcctgc acgatggtct
     1501 tgatgcccat gccgccacca tagaatatgg caaaccagta gagcagcggc gaacccggcg
     1561 tggccagcat gagcagcacc agtccgatca ccgggagcga cgttcccgcc atgcccgcaa
     1621 gcctcacatc gatgcgcttc tcgagcgcga aaaccacaat gcgcccgatg acctgaaacg
     1681 gccccaccag cgtataggcc gcaatgatca tggggagcgc aaagccgcgc tcggtgagca
     1741 gcgggatgat gtggaaggtg aggccggtga acatgaaggc gtgcgccccg tagctcagcg
     1801 ccagaagcca gaacaccggt gtggccatgg cccggcgcac ccggcccttg ggcacattcg
     1861 ctttgctgac cacaaccctc tcattcgagc ttcccagcac aaaccagtga atgagaatgc
     1921 caaagggaat ctggattgcc gcaaggacca tgagggcctg acgccagt
//
LOCUS       DAORVN010000455         1964 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_63186_length_1964_cov_2.157674, whole genome shotgun sequence.
ACCESSION   DAORVN010000455 DAORVN010000000
VERSION     DAORVN010000455.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1964)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1964)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1964
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63186_length_1964_cov_2.157674"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..281)
                     /locus_tag="PKV23_08915"
     CDS             complement(<1..281)
                     /locus_tag="PKV23_08915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85596.1"
                     /translation="MTRILGRAVLSALFRLKLKIAPKNKGFHDSAKALVSSAADSANG
                     DENMADEKVLTVPLSKMAAELSEKHEMSKKLAGELLVRRGTPVVLEGYEP"
     gene            complement(305..1252)
                     /locus_tag="PKV23_08920"
     CDS             complement(305..1252)
                     /locus_tag="PKV23_08920"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HQX85597.1"
                     /translation="MQHPTEITANRPALIEWPTITLIILTYAALATLMWFYYALPFWV
                     IMPLAGYLIALHGSLQHEVLHGHPTRNAPFNELLVAINFSLNFPYRRYRKLHLIHHND
                     ENLTDPTLDPESYYLLPEDWARLPSPMKQLYTINNTLAGRMIIGPIIGTIRFWSSEIR
                     ALAKGDTTIIKAWALHIPACVITLAYAYFICGIPLWAYVVMFAWPGIAFSLVRSFCEH
                     QAVQDLGERTIVVESSAPLSLMFLNNNLHLAHHVKPRMAWYELPAYYRAEREELLRKN
                     HGYLMHGYREIFRRYFFKPKEPVAYPHPEYLQSHRANQL"
     gene            1311..1481
                     /locus_tag="PKV23_08925"
     CDS             1311..1481
                     /locus_tag="PKV23_08925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85598.1"
                     /translation="MIASLPMYDWPEVKDATDAWWRGLSRHLRLPVELDRTGDHFAAW
                     RRADLAFSQTCG"
     gene            1506..>1964
                     /locus_tag="PKV23_08930"
     CDS             1506..>1964
                     /locus_tag="PKV23_08930"
                     /inference="COORDINATES: protein motif:HMM:NF024376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HQX85599.1"
                     /translation="MLKYIATPHYAAPGYKDADYCSFILARYDQPPDSFRGGVAVVNN
                     PDSMSGMLALKLVFAPFATDGRFFARAMQSGGHVKSMLAVRDGLADVCAIDAVCVAMA
                     RRYRPDYLEGLVEIARSPSVPGLPYVTRGGNIEAMRQGLVAAFADSDLQQC"
BASE COUNT          418 a          538 c          563 g          445 t
ORIGIN      
        1 ggctcgtagc cttccagcac caccggggtg ccgcgccgga ccagcaattc gccggccagc
       61 ttttttgaca tctcgtgttt ttcggaaagc tctgctgcca ttttggacag gggaaccgtc
      121 agtacttttt cgtcagccat gttttcatct ccgtttgccg aatcggccgc tgaggaaact
      181 agcgctttag ctgagtcgtg aaaaccctta tttttagggg ctattttcaa tttgagtcga
      241 aataacgctg ataaaacggc ccttcccaaa atccgggtca taaggactcc ggaaaacaga
      301 acgattacag ctggttagcc ctgtgggatt ggaggtactc cggatgcgga taagccacgg
      361 gttccttcgg cttgaaaaaa taccggcgga aaatttcgcg gtagccatgc atcaggtagc
      421 cgtggttttt tcgcaaaagt tcttcccgct cggcccggta ataggccgga agttcatacc
      481 aggccatgcg cggtttcaca tgatgtgcca gatgcaggtt gttgttgagg aacatcagcg
      541 aaagcggcgc cgatgattcc accacgatgg tgcgctcgcc aagatcttgc accgcctgat
      601 gttcgcagaa ggaacgcacg agggaaaaag caatacccgg ccaggcgaac atcacgacat
      661 aggcccacag cggaatgccg cagatgaaat aggcataggc aagcgtgatg acgcaggccg
      721 gaatatgcag ggcccaggct ttgattattg tcgtatcacc cttcgcaagg gcacggattt
      781 ccgatgacca gaaacgtatt gtgccgatga tcgggccgat gatcatgcgg cccgccagcg
      841 tattgttgat cgtgtaaagc tgcttcatcg gtgaaggcag tcgcgcccaa tcctcgggca
      901 gcagataata cgactccgga tcaagcgttg ggtcggtcag gttctcgtca ttatgatgga
      961 tgagatgcag cttgcggtaa cggcggtaag gaaaattgag gctgaaattt atcgccacca
     1021 gaagttcatt gaacggcgca ttgcgcgtcg ggtgcccgtg cagcacttca tgctgcagcg
     1081 agccgtgcag cgcaatgaga tagccagcca gcggcatgat cacccagaag ggcagggcat
     1141 aatagaacca catcagcgtt gccagcgccg cataagtcag gatgatcaga gtgatggtcg
     1201 gccattcgat aagcgcaggc cggttcgcgg tgatttcagt cgggtgttgc atgggcacca
     1261 attatttggc tttctggcat tgttcaagtc tcaaggaatc tcatttggcc atgatagcat
     1321 ctttacccat gtatgattgg ccggaagtca aggacgccac cgatgcctgg tggcgcggcc
     1381 tctcccgcca tctccgcctg cccgtggagc ttgaccggac gggcgaccat ttcgccgcct
     1441 ggcgccgcgc cgacctcgcc tttagccaaa cttgcggcta acctttcacc catgaattca
     1501 agggcttgct caagtatatt gcgaccccgc attatgccgc gccgggctac aaagacgctg
     1561 attactgcag tttcatcttg gcccgttatg accagccccc ggattccttc cggggtggtg
     1621 ttgccgtggt taacaacccg gattccatgt ccggcatgct ggcgctcaaa ctggttttcg
     1681 cgccatttgc cacggatggt aggttctttg ccagggccat gcaaagcggc ggacacgtaa
     1741 agtccatgct tgcggtgcgc gatggcctgg ccgatgtctg cgccattgat gcagtttgcg
     1801 tggccatggc ccggcgctac cgcccggact atctggaagg cctggttgaa atcgcccgct
     1861 caccatcggt tcccggcctt ccctatgtca cgcgcggtgg caacattgaa gccatgcggc
     1921 aggggctggt tgccgccttc gcggattcgg acttacaaca gtgc
//
LOCUS       DAORVN010000456         1958 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_63511_length_1958_cov_3.053074, whole genome shotgun sequence.
ACCESSION   DAORVN010000456 DAORVN010000000
VERSION     DAORVN010000456.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1958)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1958)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1958
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63511_length_1958_cov_3.053074"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..780
                     /locus_tag="PKV23_08935"
     CDS             <1..780
                     /locus_tag="PKV23_08935"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018699868.1"
                     /note="an AccC homodimer forms the biotin carboxylase
                     subunit of the acetyl CoA carboxylase, an enzyme that
                     catalyzes the formation of malonyl-CoA, which in turn
                     controls the rate of fatty acid metabolism; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HQX85600.1"
                     /translation="KAAFGNEEVYIEKYLVLPRHIEIQVLGDGQGNAVHLGERDCSLQ
                     RRHQKIWEEAHSPALNSIQRDEIGARVAKAMADISYEGVGTIEFLYENGEFYFIEMNT
                     RLQVEHPVTEMITGLDLVQEQIRVASGAKLQYAQSDITFAGHAIECRINAENAKTFVP
                     SPGRVDAVHFPGGLGVRVDSALYAGYRIPPYYDSLIGKLIVFGKTRTECMMRLKRAMG
                     EFVIEGVETTIPLFQRLLAEPDIIDGHYDIHWLENYLTRDA"
     gene            780..1427
                     /gene="aat"
                     /locus_tag="PKV23_08940"
     CDS             780..1427
                     /gene="aat"
                     /locus_tag="PKV23_08940"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020185262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HQX85601.1"
                     /translation="MTTITPKILLQAYAVGIFPMAETAEDSALYWVEPEERGIIPLRG
                     LRISHSLRKQVRRQTFEVRVDSAFSEVIDSCAAKTKTRKLTWINTRIRTLYNQLHKMG
                     HCHSVECWRDGKLVGGLYGVRIGAAFFGESMFSRETDASKVALVHLVARLNAGGFRLL
                     DAQFMNPHLKTLGAIVISKADYKELLPSALIAPADFHRFTQDDDPARVLELAGSG"
     gene            complement(1424..1858)
                     /locus_tag="PKV23_08945"
     CDS             complement(1424..1858)
                     /locus_tag="PKV23_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019965825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2155 domain-containing protein"
                     /protein_id="HQX85602.1"
                     /translation="MAPVWCWLLPCAAQADRIANPIAVFTGLDKITGLTTTFETNVGE
                     AKQFGGLIVKADVCYSRPATEEPKTTAFVEVDEVQLDDSLKRIFSGWMFAQSPGLNAV
                     EHPIYDVWLINCRDPAAPPPAVEEVPDLSTLQEQIEEGEPLD"
BASE COUNT          392 a          575 c          600 g          391 t
ORIGIN      
        1 aaggcggcct tcggcaatga ggaagtctac atcgagaaat acctggtgct gccgcggcat
       61 attgaaatcc aggtgctggg cgacgggcaa ggcaatgcgg tgcatctggg cgagcgcgac
      121 tgctcgctgc agcgccgcca ccagaaaatc tgggaagaag cccattcgcc ggccctgaat
      181 tcgatccagc gtgatgagat cggggcgcgc gtcgccaagg ccatggcgga catttcctat
      241 gagggcgtcg gcacaatcga attcctctat gagaacggcg agttctattt cattgaaatg
      301 aacacgcggc tgcaggtcga gcatccggtg accgaaatga tcaccggcct tgacctggtg
      361 caggagcaga tccgcgtggc cagcggggca aaactgcaat atgcccagag cgacatcacc
      421 tttgcgggtc atgccattga atgccgcatc aatgcggaaa acgccaaaac cttcgtgccc
      481 tcgccggggc gtgtggatgc ggtgcatttt cccggcggcc tcggggtccg ggttgattca
      541 gccctatatg cgggctaccg cattcctccc tattacgaca gcctcatcgg caagctgatc
      601 gtcttcggca agacgcgcac cgaatgcatg atgcgcttga aacgggccat gggagaattc
      661 gtgattgaag gggttgaaac gaccattccc ctgttccagc gtttgttggc tgaacccgat
      721 atcattgacg ggcactatga catccactgg ctggagaact acctcacgcg agatgcctag
      781 tgacgacgat cacaccaaaa attctgctgc aggcctatgc cgtcgggatt ttcccgatgg
      841 cggaaacagc cgaggacagc gcgctttact gggttgaacc ggaggagcgc gggatcattc
      901 ccctgcgcgg cttgcgcatt tcgcattcgc tgcggaaaca agtgcgccgc cagaccttcg
      961 aggtgcgcgt tgattcggct ttttccgagg tcatcgacag ttgcgccgcc aaaaccaaaa
     1021 cccgcaagct cacatggatc aacacgcgca ttcgcactct ctacaaccag ctccacaaaa
     1081 tggggcactg ccactcggtg gaatgctggc gcgacggcaa gctggttggc gggctttacg
     1141 gggtgaggat tggtgcggct tttttcggcg aaagcatgtt ttcgcgggaa accgacgcca
     1201 gcaaggtggc cctggtgcat ctggttgcca ggctgaatgc gggcggcttc cgcctgcttg
     1261 atgcccagtt catgaatccg catctcaaga cgctgggcgc cattgtcatc tcgaaggctg
     1321 attacaagga gctgttgcct tcggctctca tcgccccggc ggacttccac cggttcacgc
     1381 aggatgatga tccggcgcgc gtgctcgaac tggccggatc gggttagtcc aggggctcgc
     1441 cctcttcgat ctgttcctga agtgttgaaa gatcgggaac ttcctcaaca gcgggtggcg
     1501 gagcggcggg gtcgcggcaa ttgatcagcc agacatcata gatgggatgc tcgaccgcat
     1561 tcagccccgg gctttgggca aacatccagc cggagaaaat ccgcttcagc gaatcgtcca
     1621 gctgcacttc atcgacttcc acgaaggccg tggttttggg ttcctcggtg gccgggcgcg
     1681 agtaacacac atcggctttc acaatcaggc cgccaaactg cttggcttcg cccacattgg
     1741 tttcaaatgt cgtggtcagc ccggtgatct tgtccagccc ggtaaaaacc gcaatgggat
     1801 tggctatccg gtcagcctgg gcggcgcagg gcagcagcca gcaccacacg ggcgccacgt
     1861 cggcggcttg cgccgtgccc gtcgcgtcgc gcgcgccagt cagcgcagcg cgcacctgca
     1921 gttccgcgac cgtgggcgcg gcgtcgtggc tgcgcagg
//
LOCUS       DAORVN010000457         1952 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_63845_length_1952_cov_3.482868, whole genome shotgun sequence.
ACCESSION   DAORVN010000457 DAORVN010000000
VERSION     DAORVN010000457.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1952)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1952)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1952
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63845_length_1952_cov_3.482868"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(37..1185)
                     /locus_tag="PKV23_08950"
     CDS             complement(37..1185)
                     /locus_tag="PKV23_08950"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008836032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HQX85603.1"
                     /translation="MNTVTKLKPGKADFAAIAEALGKDFAARAEVADETDCFVADNYA
                     ALKTSGLVEASVPAELGGGGANIHELAGMLRTLAHHCSSTALAFAMHTHLTAVPSWRW
                     SHQKVAAVEPLLKRIAAEKIFLSTSGGSDWVAGAGKAERVEGGYKITARKVFSSGAPA
                     GSIFMTTAVLEEPGQEPMVLHFGIPMNSPNLRIDPVWKTLGMRGTASHDVIIEGHVVP
                     EAAVALKRKANEWHPLFQIIGTIAIPLIYSVYLGVAESARDIAVKLAKKRRSDHHILQ
                     LVGQMDSELLGARVAQSYMLDVVAQNTPGAESINQVMMGRRLVAAHAIRTVELAMESA
                     GGAGFYRSQGLERRFRDIQGARYHPMQPGQQAEYAGAMALGIPVDKIF"
     gene            1373..>1952
                     /locus_tag="PKV23_08955"
     CDS             1373..>1952
                     /locus_tag="PKV23_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010910477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HQX85604.1"
                     /translation="MPDHGSYGQFCPVAMAAEIFCSRWTALILREMICGSRRFNDLRR
                     GVPRMSPTLLSKRLVEMREAGIIEEVAGKGGKEYQLTAAGEDLKPIVMALGFWGQRWV
                     ESKLSLKNLDPSLLMWDMRRWLDPKPLPKKRCTIAFHFPDVERAQQKFWLVVEADGTV
                     DLCSSDPGFEVDLFISGSLRSMTAVWMGVTTVK"
BASE COUNT          362 a          557 c          622 g          411 t
ORIGIN      
        1 cgaaagatgg gaggcacttt aaagagcaag tctcccttaa aagatcttat cgactggaat
       61 tcctagcgcc atggcgccgg catattcggc ctgctggccg ggctgcatgg gatggtagcg
      121 cgcgccctgg atatcgcgga agcggcgttc cagtccctgg ctgcggtaga agcccgcgcc
      181 gccagccgat tccatggcaa gttcaacggt acggatggcg tgggcggcca cgagtctccg
      241 tcccatcatc acctggttga tgctttcagc gccgggggtg ttctgggcca ccacatccag
      301 catgtagctc tgggcgacgc gggcacccag gagttccgaa tccatctggc caacgagctg
      361 caggatatga tggtcgctgc ggcgcttctt cgccagcttc acggcaatgt cgcgggcgct
      421 ttcggccacc ccgagataga cgctgtagat caatggaatg gcgatagttc cgatgatctg
      481 gaacagcggg tgccattcgt tggccttgcg cttgagggcg acggccgctt ccggcaccac
      541 atggccctcg atgatgacgt catgggaggc ggtgccgcgc atgccaaggg ttttccacac
      601 gggatcgatg cgcaggtttg gcgaattcat gggaatgccg aaatgcagga ccatgggctc
      661 ctgcccgggc tcttccagaa ctgcagtggt catgaagatg ctgccggcgg gcgcgcccga
      721 ggaaaacact tttcgcgcgg tgatcttgta gccgccttcg acgcgctcgg ccttgccggc
      781 gccggcaacc cagtcggagc cgccgctggt cgagaggaag attttttccg cggcgatgcg
      841 cttgagcaaa ggctccacgg ccgccacttt ctggtggctc cagcgccagg atggcaccgc
      901 cgtgagatgg gtatgcatgg cgaaagccag tgcggtcgaa ctgcaatggt gggccagggt
      961 gcgcagcatt ccggccagtt catggatgtt tgccccgccg ccgccgagct cggctggaac
     1021 ggaagcctca accaggcctg aggttttcaa tgcggcatag ttgtcagcca caaagcaatc
     1081 ggtctcatcg gcaacttcgg ccctggcggc aaaatccttg cccagcgctt cggcaatagc
     1141 ggcaaaatcg gcttttcccg gtttcaattt ggtcacggtg ttcatggcgg tctccttgtg
     1201 cgtttttatg ctcctatcgt gcccctcgcg gctgtttgct gctagtcctg aaactgtact
     1261 aggctgctcc tacgacaagt ttcattgccg ggaagaggta cagaaactgt actgttgccc
     1321 aatccggcgt tatgttagcg gaccctgcaa taccagtgga ggagacgaag cgatgcccga
     1381 ccatggaagt tatggccagt tctgcccggt ggcgatggcg gcggaaatct tctgttcgcg
     1441 ctggaccgcc ctgatcctgc gcgaaatgat ctgtggcagc cggcgcttca acgatctccg
     1501 gcggggggtt ccgcgcatgt cgccgacctt gctttcaaag cgcctcgttg aaatgcgcga
     1561 ggccgggatc atcgaggaag tggcaggcaa gggcggcaag gaataccagt tgactgcggc
     1621 cggcgaggat ttgaagccca tcgtcatggc cctggggttc tggggccagc gctgggtgga
     1681 atcaaagctg tcgctcaaga atctcgatcc ctcgctgctc atgtgggaca tgcggcgctg
     1741 gcttgatccg aagcctttgc cgaaaaagcg ctgcacaatc gcgtttcatt ttccggacgt
     1801 cgaaagggcc cagcaaaaat tctggctggt ggttgaagcc gatggcaccg tggacctgtg
     1861 cagttccgat ccgggcttcg aggtggatct gttcatatcc ggctcgctgc gctccatgac
     1921 agcagtctgg atgggcgtga ccacggtgaa ga
//
LOCUS       DAORVN010000458         1950 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_64004_length_1950_cov_2.509763, whole genome shotgun sequence.
ACCESSION   DAORVN010000458 DAORVN010000000
VERSION     DAORVN010000458.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1950
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64004_length_1950_cov_2.509763"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(60..260)
                     /gene="rpmI"
                     /locus_tag="PKV23_08960"
     CDS             complement(60..260)
                     /gene="rpmI"
                     /locus_tag="PKV23_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019994366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HQX85605.1"
                     /translation="MPEMKTKSSAKKRFKMTASGKVRAAGAGKRHGMIKRTNKQIRNQ
                     RGTMILSPGDTGLVKIHMPYAR"
     gene            complement(392..952)
                     /gene="infC"
                     /locus_tag="PKV23_08965"
     CDS             complement(392..952)
                     /gene="infC"
                     /locus_tag="PKV23_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018267530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HQX85606.1"
                     /translation="MSIPPRRPTIVRGAAPPQKEEGNKINHRIDAREVRLIGADGQNV
                     GVVPTRQALAMAEEANLDLVEISPDAVPPVAKILDYGKFKFQEQKRAAEARKKQKVIE
                     IKEIKMRPMIDDHDYEVKMKAIRRFFEEGDKVKVTLRFRGREMAHQELGHRLLNRVKA
                     DLAEIAKVESDARFEGRQIVMIIAPK"
     gene            1072..1869
                     /locus_tag="PKV23_08970"
     CDS             1072..1869
                     /locus_tag="PKV23_08970"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQX85607.1"
                     /translation="MAGVDFEQGPGGVKLAFLQDGAADDLGRCGFFWLGGFMSDMMGS
                     KAEALAALARETRRPGLRFDYSGHGQSGGQFTDGTISDWLEQSIHMFIEKTRSKRVIV
                     GSSMGGWLALLLARVLLRDDPRAARRMAGIVLIAPAADMTSDLMLNTFPPEALEQLKA
                     RGVYERPSDYGEPMPITFKLLEDGAKHLLLKTGLELPCPVRILQGSSDPDVPPSHALK
                     VFEALSGPDITMTLIKGGDHRLSTPGQLTLIRETALQLAERADGITP"
BASE COUNT          341 a          596 c          577 g          436 t
ORIGIN      
        1 tttcttgtgg gatttgcggg cgacagtgcc gccttttgaa cgtggcatgg gagctctcct
       61 taacgggcgt agggcatatg aattttgacc agtccggtgt caccggggga caaaatcatg
      121 gtgccgcgct ggttgcggat ctgcttgttg gtgcgcttga tcatgccgtg gcgcttgcca
      181 gctccggcgg cacgaacctt gccggaagcg gtcattttga agcgcttttt ggccgaggat
      241 ttcgttttca tctcgggcat ttggcttaag tcctttcaaa gcgataagaa gccgccatgg
      301 catgcccttt acatggccag gcggcttagt ggggggtgtt aagcagaaat tcgggggcga
      361 tgcaacccat cggattgcac ccccttaagg cctatttcgg cgcgataatc atcacgatct
      421 ggcggccttc aaagcgcgcg tcggattcaa ccttggcgat ttcagccaga tcggccttca
      481 cccggttcaa aagccggtgg cctagctcct ggtgggccat ttcgcggccc cggaaacgca
      541 gcgtcacctt gaccttgtcg ccctcttcga agaaccgcct gatggccttc atcttgacct
      601 cgtagtcgtg gtcatcgatc atcgggcgca tcttgatctc cttgatctca atgacctttt
      661 gcttcttgcg ggcctcggcg gcccttttct gttcctggaa tttgaatttg ccgtaatcga
      721 ggatcttggc aaccggggga accgcgtccg gcgagatttc gaccagatcg agattggctt
      781 cctcggccat ggcaagcgcc tggcgggtgg gcacaacgcc gacattctgt ccgtcggctc
      841 cgatgaggcg cacttcgcgc gcatcaatgc ggtggttaat cttgttacct tcttcttttt
      901 gcgggggggc ggcaccgcgt acaatcgttg gcctccgggg gggtatactc aagctctatg
      961 cttctccatg ttaaggccgt atcgattccg cgatgggccg gttaagaacc ccgctataga
     1021 ccttgaagcg ggcctgccgt caaggagggc tgaaaggatt ggataattta gatggcgggt
     1081 gtggattttg agcaaggccc gggcggcgta aaactggcct ttctgcagga tggcgccgcc
     1141 gatgatctgg gccgctgcgg cttcttctgg ctgggcggtt tcatgtccga catgatgggc
     1201 agcaaggccg aggcgctcgc cgcccttgcc cgcgaaaccc gaaggccggg cctgcgcttt
     1261 gattattccg gccatggcca gtccggcggc cagttcaccg acggcaccat ttccgactgg
     1321 ctcgaacagt ccatccacat gttcatcgaa aagacccgct ccaaacgcgt catcgtcggt
     1381 tcgagcatgg gcggctggct ggcgcttctg ctggccaggg tcctgctgcg cgatgacccc
     1441 cgcgccgccc ggcgcatggc cggtatcgtg ctgatcgccc ccgccgccga catgaccagt
     1501 gatctgatgc tgaacacatt cccgcccgaa gccctcgagc aactgaaggc ccgcggcgtc
     1561 tatgagcgcc cctcggacta tggcgagccc atgcccatca ccttcaagct tctggaagac
     1621 ggcgccaaac acctgctgct gaaaaccggg cttgaattgc cctgtcccgt gcgcatcctc
     1681 cagggcagca gcgacccgga cgtgccgcct tcccatgcgc tcaaggtttt cgaggccctc
     1741 tccggcccgg acatcaccat gaccctgatc aagggcggcg atcaccgcct gtcgacaccc
     1801 gggcaattga cgctcatccg tgaaaccgcc ctgcagctcg ccgaacgggc cgacggcatt
     1861 acgccgtgag tctgtccaag agggcgcgca agccttcccg ataactcgga tagatcagca
     1921 tatagttcaa ctcggctttg atgcgcccgt
//
LOCUS       DAORVN010000459         1941 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_64541_length_1941_cov_3.250795, whole genome shotgun sequence.
ACCESSION   DAORVN010000459 DAORVN010000000
VERSION     DAORVN010000459.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1941
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64541_length_1941_cov_3.250795"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            53..>1941
                     /locus_tag="PKV23_08975"
     CDS             53..>1941
                     /locus_tag="PKV23_08975"
                     /inference="COORDINATES: protein motif:HMM:NF028476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HQX85608.1"
                     /translation="MFGFQCAADGGGYTWFGNSRENQTTAWSNDPVSNRLSEAIYVQD
                     EKDGILLSPTLAPLKSPDGTHVARHGFGYSVFERDAHRLRMELLQTLPLTDTVKLSRL
                     RLTNNSPTARTFSVTFYAEWVLGPNRAASAPYLTTGIDEQTGAMFARNPWNVQMGEQV
                     AFADMGGKQTGWSGDRREFLGAHGRLAAPKALTSRQALSNRTGAGLDPCCALQANVTL
                     EAGGSTELVIMLGAASNAAEARSLIARYRSNSVDNVLAEVKQYWSETLGHVQVKTPDR
                     ALDIMLNGWLLYQTLSCRMWARSGFYQTSGAYGFRDQLQDSMALLTTRPAIAREHILR
                     AAARQFVEGDFQHWWLPSTGMGVRTRISDDTVWLANCVAQYVKVTGERDLLDEIVPFI
                     EGQSLAPGEHDAFFQPRHSEESASLYEHCARALDRSLPFGAHGLPLMGTGDWNDGMNR
                     VGEGGKGESVWLGWFLFTTLKTFAPVAAERGETKRAAAWRDRAKGLRAALDRHAWDGR
                     WYRRGFFDDGTVLGSAQSEECQIDSIAQSWSVLSGGGTAERAATAMDESYRQLVRQDD
                     SLALLFTPPFDKTPKDPGYIKAYPPGIRENGGQYTHGAIWSVFAHAGLGQADRALEVF
                     SLLNP"
BASE COUNT          403 a          581 c          588 g          369 t
ORIGIN      
        1 ccaaagcgac cgaccgaccc cagcgccctg gatcaatgtc attgctaatc cggtgtttgg
       61 atttcagtgc gccgcagatg gcggcggata cacctggttt ggaaacagcc gggagaacca
      121 aaccacggct tggagcaatg atcccgtcag caaccggctc agcgaggcca tctatgtcca
      181 ggacgaaaaa gacgggatcc tgctatctcc gaccctggcc ccgctgaagt ctcctgacgg
      241 cacacacgtt gcgcggcatg ggttcggcta cagcgtgttc gagcgtgacg cacacaggct
      301 gcgcatggaa ctgctgcaga cgttgccgct cacggacacc gtcaaactat cgaggctcag
      361 actgacaaac aactcgccaa ccgccagaac cttctcagtg acattctatg ccgaatgggt
      421 tctgggacct aatcgcgccg cttccgcacc ctatttgacg actggaattg acgaacagac
      481 gggggcgatg tttgcgcgca atccgtggaa tgtgcagatg ggcgaacagg tcgcatttgc
      541 cgacatggga ggcaagcaaa cgggctggag tggcgatcgc agggagtttt tgggcgccca
      601 tggccggctt gcggcaccga aggcactgac atcacgacaa gcgctttcaa accgcacagg
      661 cgccggactg gatccttgct gtgccctgca ggccaacgtc acgcttgaag cggggggttc
      721 caccgaactg gtgatcatgc tgggcgccgc gtcgaatgct gctgaagccc gatccctcat
      781 cgcacgctac aggtcgaaca gcgtcgacaa tgtactagcc gaagtgaaac agtactggag
      841 cgaaaccctt ggccacgtgc aggtcaagac acctgaccgc gcccttgata tcatgctcaa
      901 tggctggctc ctgtatcaga ccctcagttg ccgcatgtgg gccaggtcgg gattctacca
      961 gaccagcgga gcctatggct tccgcgacca gttgcaggac tcgatggccc tgctcaccac
     1021 gcgaccggca attgcacgcg agcatatcct gcgcgccgcc gcccgtcagt ttgtcgaggg
     1081 cgacttccag cattggtggc ttccctccac cggcatgggt gtcagaacgc gcatatccga
     1141 tgacaccgtg tggctggcca attgcgtggc ccaatatgtg aaagtgaccg gggaacggga
     1201 tcttctagac gaaattgtgc cattcatcga aggacaatcg ctcgcacccg gcgaacacga
     1261 cgcgtttttc cagccaaggc actcagagga atcagccagt ctctatgaac attgcgcccg
     1321 tgccctggac cgcagcttgc cctttggcgc acatggattg ccgctcatgg gcaccggtga
     1381 ctggaatgac ggcatgaacc gggttggcga aggcggaaag ggcgaaagtg tctggcttgg
     1441 ctggttccta ttcacgactc tgaaaacctt cgccccggtc gcggccgaac gcggagaaac
     1501 caaacgtgcc gcagcctggc gcgaccgcgc caaggggttg cgggctgcgc ttgacagaca
     1561 tgcgtgggac gggcgctggt accggcgcgg gttttttgat gatggcaccg tgctgggttc
     1621 ggcacagagc gaggaatgcc agatcgattc cattgcgcag tcgtggtcgg tgctgtcggg
     1681 cggcggcacc gcggagcgcg ccgcaactgc catggatgaa tcctaccggc agctggttcg
     1741 tcaggatgat tcactggcgc tcctctttac gccgccattt gacaaaactc cgaaggaccc
     1801 gggatatatc aaggcctatc ctcccggcat ccgtgaaaat ggcgggcaat atacccatgg
     1861 cgccatttgg tcggtctttg cccatgccgg cctgggacag gctgataggg cgctggaagt
     1921 gttctccctg ctcaacccga t
//
LOCUS       DAORVN010000460         1940 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_64603_length_1940_cov_2.674271, whole genome shotgun sequence.
ACCESSION   DAORVN010000460 DAORVN010000000
VERSION     DAORVN010000460.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1940
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64603_length_1940_cov_2.674271"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..758)
                     /gene="gguC"
                     /locus_tag="PKV23_08980"
     CDS             complement(<1..758)
                     /gene="gguC"
                     /locus_tag="PKV23_08980"
                     /inference="COORDINATES: protein motif:HMM:NF040903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GguC family protein"
                     /protein_id="HQX85609.1"
                     /translation="MNIVQILDSKGKRRVGLVNAEKIILLKKVARTTDLARLALKDGT
                     KLLNAAKSLTTSATENYAAAIKENRLLPPVDHEDPAHCMIAGTGLTHLGSASARDSMH
                     AKLEGKADDLTDSLKMFKMGLEGGKPAKGQAGVQPEWFYKGDGSWLVAPGAPLPKPSF
                     ALDGGEEPELAGLYMIDGKGNPVRLGFALGNEYSDHITERQNYLFLAHSKLRHCAIGP
                     EMIAGAEPNNISGMSRINRKGKTIWEKPFLTGEA"
     gene            complement(809..1840)
                     /locus_tag="PKV23_08985"
     CDS             complement(809..1840)
                     /locus_tag="PKV23_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004110222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline racemase family protein"
                     /protein_id="HQX85610.1"
                     /translation="MRTSKSIHLVNCHAEGEVGDVIVGGVAPPPGNTIWEQSRFIAGD
                     QTLRNFVLNEPRGGVFRHVNLLVPPKHPEAQMGFIIMEPEDTPPMSGSNCICVATVLL
                     DTGIIPMVEPVTHLKLEAPAGLVEITAHCSNGKANRITLRNVPAFADRLQVPLEIEGH
                     GTIRVDTAFGGDSFVLAEAKSFGFAMQPDEAREIAVMGRKIAAAANAQLGFSHPALPD
                     WKHFSFCFMTGPLEHIDGALSSRNACVVKPGKLDRSPTGTGCSALMAVLHAKGLMKTG
                     DTFIGRSIIESRFVGRIEAETIVGNHRAIIPTISGSAWITGQTTLMLDPGDPWPAGYK
                     IADTWPKEP"
BASE COUNT          368 a          527 c          619 g          426 t
ORIGIN      
        1 ttggcctcgc cggtgaggaa gggcttttcc cagatcgttt tgcccttgcg gttgatgcgg
       61 ctcatgccgg aaatattgtt tggctcagcg cctgcgatca tttccgggcc gatggcgcag
      121 tggcgcaact ttgagtgcgc cagaaagaga taattctgcc gctcggtgat gtggtcggaa
      181 tactcgttgc ccagcgcgaa gcccaggcgc acgggattgc ccttgccgtc aatcatgtag
      241 aggccggcca gttccggctc ctcgccgccg tcgagggcga aggatggttt gggaagggga
      301 gcacccgggg ccacaagcca ggagccatcg cccttgtaga accattcggg ctgcacgccg
      361 gcctggccct tggcgggctt gccgccctcg aggcccatct tgaacatctt gagggagtcg
      421 gtgagatcgt ccgccttgcc ttcaagcttg gcatgcatgg aatcgcgcgc cgatgcggag
      481 cccagatgag tgaggccggt gccggcgatc atgcaatgag ccggatcttc atggtcaacc
      541 ggcggcagca gacggttttc cttgatggcc gccgcataat tttcggttgc gctggtggtc
      601 agggactttg cggcattcaa aagcttcgtg ccgtctttca aggcgagcct tgccagatcc
      661 gttgtccggg cgaccttttt caggagaatg attttctcag cgtttacgag accaacacgg
      721 cgcttaccct tggaatcgag aatttgaacg atattcatca tgcctcccag ccggtttttt
      781 catattgtat gataaattat tcgggatgtc atggctcttt gggccaggtg tcggcgattt
      841 tatagccggc gggccatggg tcgccgggat cgagcatcag ggtggtctgg ccggtgatcc
      901 aggcggaacc ggaaatggtg gggataatcg cccggtgatt gccaacaatt gtttcggctt
      961 caattcgccc cacaaagcgg ctttcgatga tggagcggcc aataaaagtg tcgccggttt
     1021 tcatcaggcc cttggcatgg agcacagcca tcagggcgga acagccggtg ccggtgggtg
     1081 aacggtcgag cttgcccggc ttcaccacgc aggcattgcg gctggagagt gcgccatcaa
     1141 tgtgctccag ggggccggtc atgaagcaga aagaaaaatg cttccagtcg ggcaatgccg
     1201 ggtgggaaaa tcctaattga gcgttggcgg cggcagcgat tttccggccc atgacagcaa
     1261 tttcacgggc ctcgtcgggc tgcatggcaa agccgaatga ttttgcctcg gccagcacaa
     1321 agctgtcgcc gccaaaggcg gtatccaccc gtattgtccc gtggccttcg atttccaggg
     1381 gcacctgcag gcggtcggcg aaggcgggga cattgcgcag tgtgatgcgg ttggccttgc
     1441 cgttgctgca gtgggcggtg atttcaacga ggccggcggg agcttccagc ttcaaatgcg
     1501 tcacgggttc caccatggga atgatgccgg tatcgagcag caccgttgcc acgcaaatgc
     1561 agtttgagcc ggacatgggg ggcgtgtctt ccggctccat gatgatgaag cccatctgcg
     1621 cctccgggtg cttgggcggc accagcaggt tcacatggcg aaacaccccg ccgcggggct
     1681 cgttcaggac gaaattacgc aaagtctgat cgccggcgat gaaacgtgat tgttcccaga
     1741 tcgtgttgcc gggcggcggc gccacgcccc ccacgatgac gtcgcccacc tcgccttcgg
     1801 cgtggcaatt gacgaggtgg atggatttcg aggtgcgcat ttgcttgggt ttaccgcagg
     1861 ggtttgaatt tcataataaa ataataagta acggcttgca ctttcgggct ggaggcacca
     1921 tttcgacaca atcagcaccg
//
LOCUS       DAORVN010000461         1940 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_64608_length_1940_cov_2.489125, whole genome shotgun sequence.
ACCESSION   DAORVN010000461 DAORVN010000000
VERSION     DAORVN010000461.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1940
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64608_length_1940_cov_2.489125"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..564)
                     /locus_tag="PKV23_08990"
     CDS             complement(<1..564)
                     /locus_tag="PKV23_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020050995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HQX85611.1"
                     /translation="MKYLHLGKTGPQVSALGLGRMGMSGMYGPSDRAESIATIHAALE
                     AGITLFDTGDFYGMGHNEMLIGEALKGRNRDKAIISVKFGALRDPAGGWSGSDARPGA
                     VKNFLAYTLQRLGVDYIDVYRPARLDANVPIEDTIGAIADLVKAGYVRHIGLSEVGAN
                     SIRRAAAIYPIVDLQIEYSLISRGIEAA"
     gene            662..>814
                     /locus_tag="PKV23_08995"
     CDS             662..>814
                     /locus_tag="PKV23_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018446637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HQX85612.1"
                     /translation="MRRLPLADLEAFAAVAEMRSFRKAAALRGISATSLSDAMRRLEA
                     RLGVRLL"
     assembly_gap    815..824
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <825..1572
                     /locus_tag="PKV23_09000"
     CDS             <825..1572
                     /locus_tag="PKV23_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018495426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HQX85613.1"
                     /translation="RTTRSVTLTDAGQQLLARLSPALGDIASMLDGLGPAGDTPSGTL
                     RLNVPGIVARYILPPIVVRFLKAYPRVSIEIVAEDVYSDVFAKGFDAGVRYDERLELD
                     MIAVPIGPRSQRFIAVASPAYLKIHGTPSHPEDLLKHTCIRHRFLSGVMPTWEFEREG
                     VVVRVDPPSVIITNSLDISRAAAVAGLGIMTSFSEFTEAEVASGALQPVLEDWLPPFS
                     GPFIYYPSRKHMPPPLRAFLDFLKTEMAAT"
     gene            1604..>1940
                     /locus_tag="PKV23_09005"
     CDS             1604..>1940
                     /locus_tag="PKV23_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003542167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HQX85614.1"
                     /translation="MVNLHDSWKAPLQAEFDAPYMAALKEFLVAERDKGKRIFPKGSE
                     WFHALDATPLEQVRVVILGQDPYHGEGQAHGLCFSVKPGVRPPPSLINIYKEMKSDLG
                     LEPPSHGNLE"
BASE COUNT          370 a          544 c          607 g          409 t
ORIGIN      
        1 ggcggcttca atgccccgcg aaatcagtga atattcaatc tgcagatcga cgatggggta
       61 gattgctgcg gcgcgccgga tgctgttggc gcccacttcg gacagcccga tgtggcgcac
      121 gtaaccagcc ttcaccagat cggcaatggc gccgatggta tcctcgatcg gcacgttcgc
      181 gtccaagcgg gcagggcgat agacatcgat gtagtccacg cccaggcgct gcagggtgta
      241 ggcgaggaag tttttgacag cgccaggcct tgcatcagat ccgctccagc cgccggcagg
      301 atcgcgcaag gcgccaaact tgacgctgat gatggccttg tcgcggttcc tgcccttgag
      361 agcctcgcca atgagcattt cattgtggcc catgccgtag aagtcgccag tgtcgaagag
      421 ggtaatgccg gcttccaggg cggcgtggat ggtggcgatg ctttcggccc ggtcggacgg
      481 gccatacatt ccggacatgc ccatgcggcc aaggccgagg gctgaaacct gcgggccggt
      541 tttgccgagg tgaagatatt tcatttgcgt tctcctgttt ccgatttctc ctccttctaa
      601 ccgatggaga tatgtgcgat aatccggtca gtcattaata ggttgtacgg aaaaacgcac
      661 aatgagacga ttgcctctgg ctgacctgga agcgtttgcg gcggtggccg agatgcgaag
      721 ttttcggaag gccgcggcgc tgcggggcat ttccgccacg tcgctgagcg atgcgatgcg
      781 ccggctggag gccagactgg gggtgaggct gctcnnnnnn nnnnccgcac cacgcgcagc
      841 gtaacgctca ctgatgccgg gcagcagctt ttggcgcggc tttcgcctgc gctcggggac
      901 attgcctcca tgctggatgg cctcggccca gccggcgaca cgccaagcgg aacgcttcgg
      961 ctgaatgttc cgggcattgt ggcgcgctat atcctgccgc cgattgtggt gcgttttctc
     1021 aaggcatacc cgcgcgtttc aatcgaaatt gttgccgaag acgtctattc ggatgttttc
     1081 gcaaagggct tcgatgcggg cgtgcgctac gatgagaggc tggagctgga catgattgcc
     1141 gtgccgatcg ggccacggtc acagcgtttc atcgcggtcg cctcgccggc ctatttgaaa
     1201 atacacggaa caccttcgca tcccgaggac ctgctcaagc acacgtgcat ccggcaccgg
     1261 tttttaagcg gcgtcatgcc gacctgggaa ttcgagcgcg aaggagttgt ggtgcgcgtt
     1321 gatccgccaa gcgttatcat tacaaattca ctggatattt ccagagctgc ggcggtggcc
     1381 gggcttggca tcatgacgtc gttcagcgag tttaccgaag ccgaagtggc aagcggcgcg
     1441 ttgcagccgg tgcttgaaga ctggctgccg ccattctcag gccccttcat ctattatccc
     1501 agccgcaagc acatgccgcc gcccctgcgg gcgtttctgg attttctgaa aaccgaaatg
     1561 gcggccactt gatctgaggc ccgcgaatcc gccatgaagg ggcatggtca atctgcatga
     1621 cagctggaag gcgccgcttc aggcggagtt cgatgcgccc tacatggcgg cgctgaagga
     1681 attcctggtt gccgaacggg acaagggcaa acggattttt cccaagggtt ccgagtggtt
     1741 ccacgcactt gatgcaacgc cgcttgaaca ggtgcgcgtc gtgatactag ggcaggatcc
     1801 ctatcacggc gaagggcagg cccatgggct ttgcttttcg gtgaaaccgg gggtaaggcc
     1861 gccgccatca ctgatcaata tctacaagga aatgaagagc gatcttggcc ttgagccgcc
     1921 gtcccatgga aatcttgaag
//
LOCUS       DAORVN010000462         1937 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_64792_length_1937_cov_3.017535, whole genome shotgun sequence.
ACCESSION   DAORVN010000462 DAORVN010000000
VERSION     DAORVN010000462.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1937
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64792_length_1937_cov_3.017535"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            38..1840
                     /locus_tag="PKV23_09010"
     CDS             38..1840
                     /locus_tag="PKV23_09010"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HQX85615.1"
                     /translation="MTEFWTHLGADERLYAKYKAIDPASLNTEQRQAHKNAIRNFVLG
                     GAELTGAARERFAAIQERQAELSQKFSENALDATDAYSYFATAEELDGVPDDIRQTAR
                     DAALALGKEGYRLTLKMPCYLPVMQFARSAALREKLYRAYVTRASDQADADAGKFDNS
                     SIIREILALRLEESRLLGYSNFGELSVVPKMADSPAQVISFLRDLAVKARPFAEKDLA
                     DMRQFAAEHLGLADPQPWDWPFVSEKLKEARYSFSEQEVKQYFTAPKVLGGLFKIAET
                     LFDVRISQDQAPVWNPGVEFYRIERQTVQPGSEVAGRTLVGQFYLDPAARTGKRGGAW
                     MDDVRARWLRPDNNQLQTPVAHLVCNFAQGMEVDGVKKPPLLTHDDVTTLFHEFGHGL
                     HHMLTQVNERDVSGISGVEWDAVELPSQFMENFCWEWDVLRHMTSHVDTGAPMPRPLF
                     DKMLEAKNFQSGLQTLRQIEFSLFDMLLHTSHEPMADIMGLLEQVREEVAVMRPPTWS
                     RTAHTFSHIFAGGYAAGYYSYKWAEVLSADAYAAFEETAGNDGLPNLETGRKYREAIL
                     EAGGSRPAIESFKAFRGREPTLDALLRHQGMAAS"
BASE COUNT          423 a          559 c          584 g          371 t
ORIGIN      
        1 ggagttgcgg gccgcctaca acgaagcact gcccaaggtg accgagttct ggacacatct
       61 gggcgccgac gagcgcctgt acgccaaata caaggccatt gatcctgcgt ccctcaatac
      121 cgagcaacgc caggctcaca agaacgcgat ccgcaacttt gtgctaggcg gcgccgaact
      181 cacgggcgcc gcgcgcgaac ggtttgccgc cattcaggag cgccaggccg agttgagcca
      241 gaaattcagc gaaaacgcgc tggacgcgac cgatgcctac tcgtatttcg ccacagccga
      301 agaactggac ggcgtgcccg atgacatcag gcaaacggca cgcgatgcgg cgctggcttt
      361 aggcaaagaa ggctacaggc tcacgctcaa aatgccctgc tacctgcccg tgatgcaatt
      421 tgcccgcagt gccgccttgc gcgaaaaact ctatcgtgct tacgttaccc gcgcttccga
      481 tcaggccgac gcggatgcgg gcaagttcga caactccagc atcatccggg agatcctggc
      541 acttcggctg gaagaatcca gactgctcgg ctacagcaac tttggtgaat tgtcagtggt
      601 tcccaaaatg gcggactctc ccgcacaagt catctcgttt ttgcgggatc tggcggtaaa
      661 agccaggccc ttcgccgaaa aagacctggc tgacatgcgc cagtttgcgg cagagcattt
      721 gggtctggcc gacccgcaac catgggactg gcccttcgtc agcgaaaagc tcaaggaagc
      781 gcggtattcc ttcagcgagc aggaagtcaa acagtacttc acggcgccca aggtgctggg
      841 tgggctgttc aagatcgccg aaaccctgtt tgacgtgcgc atcagccagg atcaggctcc
      901 ggtctggaac ccgggcgtgg agttttaccg catcgaacgc cagaccgtac aacccggcag
      961 cgaggtggcg ggccgtactc ttgtgggtca gttttacctg gacccggccg cccgaactgg
     1021 caaacgtggc ggcgcgtgga tggatgatgt tcgcgcccgc tggctgcgcc ccgacaacaa
     1081 ccagttgcag acgccagtgg cgcacctggt ctgcaatttt gcccagggca tggaggtgga
     1141 tggagtcaag aaaccacccc ttctgacgca tgacgatgtc accacgctgt ttcacgagtt
     1201 cggccacggc ctgcaccaca tgttgaccca ggtgaacgag cgcgacgtgt cgggtatcag
     1261 cggcgtggaa tgggatgcag tggagttgcc cagccagttc atggagaact tctgctggga
     1321 atgggatgtg cttagacaca tgacatctca cgtcgatacc ggtgcgccca tgccgcgccc
     1381 gctgtttgac aagatgcttg aagcgaaaaa ttttcagagc ggtctgcaga cgctgcggca
     1441 aattgagttc tccctgttcg acatgttgtt gcatacaagc cacgaaccca tggcggacat
     1501 catggggctg ctggaacagg ttcgcgagga agtagccgtc atgcgcccac ccacctggag
     1561 ccgcaccgcc catacgttca gtcatatttt tgctggcggc tatgccgccg gctactacag
     1621 ctacaaatgg gccgaggtgc tgagcgccga tgcttatgcg gcgttcgagg aaacagccgg
     1681 taacgacgga ttgccaaatc tcgaaacggg tagaaaatac agggaagcca ttctggaggc
     1741 cggcggaagt cggccggcca ttgagtcttt caaggcattt cgggggcgcg agccgactct
     1801 ggatgccctg ttgaggcatc agggtatggc ggcaagttag tcagaattct gttcaagcaa
     1861 gggtagacac aatgaaacca tcgttggcac gtccatttgt tgaaaccggc ttggtcacca
     1921 ggctggggct tggcgcc
//
LOCUS       DAORVN010000463         1934 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_64987_length_1934_cov_3.000532, whole genome shotgun sequence.
ACCESSION   DAORVN010000463 DAORVN010000000
VERSION     DAORVN010000463.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1934)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1934)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1934
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64987_length_1934_cov_3.000532"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..479
                     /locus_tag="PKV23_09015"
     CDS             <1..479
                     /locus_tag="PKV23_09015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85616.1"
                     /translation="QQALGSLETRYSELAQSCESLFAATDDTQRRLHDQSMQLNSELQ
                     KNQEAQQQMLSEMFLVFDKQLEKLSSELNHKIDSLAARTSEDNHMLANDLIRRVQQLA
                     EEARADSDKVASVLEARLARSEAHAEKENRRHIEVFADGFSDIADRLLALRGVQNG"
     gene            complement(537..612)
                     /locus_tag="PKV23_09020"
     tRNA            complement(537..612)
                     /locus_tag="PKV23_09020"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(577..579),aa:Val,seq:tac)
     gene            complement(722..997)
                     /locus_tag="PKV23_09025"
     CDS             complement(722..997)
                     /locus_tag="PKV23_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020178883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HQX85617.1"
                     /translation="MNKNELIAKVAKDTRLTRGEAGEAVEATIENIMRALRQGDDVRL
                     IGFGVFTVTRRKAGEARNPQTGKLIRLPASKRAKFKAGKLLKEAVNG"
     gene            complement(1211..>1934)
                     /locus_tag="PKV23_09030"
     CDS             complement(1211..>1934)
                     /locus_tag="PKV23_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014890256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HQX85618.1"
                     /translation="KKIKVTPDVVNQYLGVQKFKHGEAEREDQVGVVTGLAWTEVGGE
                     LLTIEALMMPGKGKMTVTGNLKDVMKESISAASSYVRSRAPSIGVKPPVFDKKDIHVH
                     VPEGATPKDGPSAGIAMVTAIVSVMTGIPVRKDVAMTGEITLRGRVLAIGGLKEKLLA
                     AVRGGIKKVMIPEENAKDLAEIPDSVKNGMEIIPVAMMDDVLKQALVRMPEPIEWDEV
                     AEEAAAAARALAEKSGEGARAH"
BASE COUNT          391 a          593 c          560 g          390 t
ORIGIN      
        1 cccagcaggc cctggggtct cttgaaacgc gctacagcga acttgcccaa agctgtgaaa
       61 gcctgtttgc cgcaactgac gacacgcagc gccggctgca tgaccagtcg atgcagctga
      121 attccgagtt gcagaagaac caggaagccc agcagcaaat gctgtcggaa atgttcctgg
      181 tattcgacaa gcagctggag aagctgtcct ccgaactgaa ccacaagatc gattcactgg
      241 cggcgcgcac cagcgaggac aaccatatgc tggccaacga cctgatccgg cgagtccagc
      301 aattggccga agaagcccgc gctgacagtg acaaggtggc gtccgtcctc gaggcccgcc
      361 tggcgcgttc ggaagcccat gccgaaaagg aaaaccgccg gcacattgaa gtgttcgccg
      421 acggcttttc ggacattgcc gaccggctgc tggcgctccg cggcgttcag aacggctaga
      481 ttgggtcagc agaaagtcgt tcactagaac gctgcccgga ctgcttttct gagggatggt
      541 gggcgatgag agactcgaac tcccgacatc ttcggtgtaa acgaagcgct ctaccaactg
      601 agctaatcgc cctcttccgc cactccttag ccagatgatt tgggcattgc aagcgccata
      661 agaaaccccg cgcgattctg agggcgcgcg gggctgatag gtctgaaatc gccgtcagtt
      721 gttagccgtt aacagcctcc ttaagtagtt tgcctgcctt gaattttgcc cgttttgagg
      781 cgggaagccg gatgagtttc ccggtctgcg gattgcgcgc ctcgcccgcc ttgcgccggg
      841 tcacggtgaa gacgccgaag ccgatgaggc ggacatcgtc gccctggcgc agggcccgca
      901 tgatgttctc gattgtggcc tccacggcct cgccagcctc gccgcgggtc aggcgcgtat
      961 ccttggcgac cttggcaatc aactcgttct tgttcatgtc ttcgcactcc cggaacttaa
     1021 gggggaagaa agcctgcaaa tcaaatgccg tgccaacagg aaacgacaaa ttgcgtggga
     1081 ttcgcaagag tcaggcgaag aaaaatatat tcaggagtca gatcaacggc ttatggaggg
     1141 gcgcatataa aagcaggcgg agcaaggatg ctccgcctgc ggattatttg aaaaagtgat
     1201 tcgaaaggcg tcagtgggcc cgggcgccct cgcctgactt ctcggccaga gcccgggcgg
     1261 cggcagcggc ttcctcggcc acttcatccc actcgatggg ctcgggcatg cgcaccaggg
     1321 cctgcttcag gacatcgtcc atcatggcca ccggaatgat ttccatgccg tttttcaccg
     1381 agtccggaat ctccgccagg tccttggcgt tttcctccgg aatcatcact ttcttgatgc
     1441 cgccgcgaac cgcagccagc agcttttcct tcaggccgcc aatggccagc acccggccgc
     1501 ggagcgtaat ttcgcccgtc atggccacgt ccttgcgcac cggaatcccg gtcatcaccg
     1561 acacgatggc ggtcaccatg gcaatacctg cggacggacc atccttgggg gttgcccctt
     1621 ccggaacgtg cacgtggatg tccttcttgt cgaagaccgg cggcttgacg ccaatcgaag
     1681 gggcgcgcga gcggacatag gaggaagcag ccgagatcga ctccttcatc acgtccttca
     1741 ggttgcccgt caccgtcatc ttgcctttgc ccggcatcat gagggcttcg atggtgagca
     1801 gttcgccgcc cacttcagtc caggcaaggc cggtcacgac gcccacctgg tcttcacgtt
     1861 cggcttcgcc gtgcttgaac ttctgcacgc cgagatactg gttcaccacg tccggggtca
     1921 ccttgatctt cttt
//
LOCUS       DAORVN010000464         1929 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_65290_length_1929_cov_3.501067, whole genome shotgun sequence.
ACCESSION   DAORVN010000464 DAORVN010000000
VERSION     DAORVN010000464.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1929
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65290_length_1929_cov_3.501067"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..448)
                     /locus_tag="PKV23_09035"
     CDS             complement(<1..448)
                     /locus_tag="PKV23_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009494022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HQX85619.1"
                     /translation="MNSFSELNLSAPILKAIAAEGYDKPTPIQARAIPELLKGRDLLG
                     IAQTGTGKTAAFALPILERLSHATERVAKNSCKVLVLSPTRELASQIGESFKTYGRNL
                     KVTRAVVFGGVSIGKQIDILRGGVDVVVATPGRLVDLIERRALTLAN"
     gene            complement(701..1201)
                     /locus_tag="PKV23_09040"
     CDS             complement(701..1201)
                     /locus_tag="PKV23_09040"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006115728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HQX85620.1"
                     /translation="MSEAKVITAYRPCVCVMLLNRQGLVWIGRRFEKKNDEGTGHWWQ
                     MPQGGIDGSEDPAAAALRELEEETAVRSAKIIAEAPGWYNYDLPEHLIGKSWKGKYRG
                     QTQKWFAARFLGEDSEINLAPPGHKQEFDQWRWAGMDEIVGMIVPFKKPVYEQVVKAF
                     RHLGAA"
     gene            complement(1201..>1929)
                     /locus_tag="PKV23_09045"
     CDS             complement(1201..>1929)
                     /locus_tag="PKV23_09045"
                     /inference="COORDINATES: protein motif:HMM:NF016623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HQX85621.1"
                     /translation="PSDVYARNTPLGVIHSERPKIAILLGGMGLNAKLTQQAIKNLPG
                     DVTFAFAPYGENLQSQVNKARADGHEVLLQLPMEPVGYPANNPGPKTLLAGGTAEENL
                     NALHWHMTRFAGYTGITNYMGARLLATPEALKPVMAEMRKRGLVYLEDASTALTVSKS
                     VAQQTKLPERRAQVVIDADPTVQSIAAALELLEGEAKANGFAIGTGSGLEVTVDAVKL
                     WAEQLQERGILLVPVSAMFKGRLG"
BASE COUNT          376 a          544 c          611 g          398 t
ORIGIN      
        1 cattggccag cgtcagggcg cggcgctcga tgagatcaac gaggcggccc ggggttgcga
       61 caacgacatc aacgccgcca cgcaggatat cgatctgctt gccgatggag acgccgccga
      121 aaaccacggc gcgggtaact ttcaaattgc ggccataggt cttgaagctt tcaccgatct
      181 gcgaggcgag ctcgcgggtg ggcgaaagca cgagaacctt gcaggaattt ttcgccacac
      241 gctcggttgc gtgcgacagg cgctcaagga tgggaagcgc gaaggctgcc gtcttgccgg
      301 tgcccgtctg ggcaatgccg agcagatcgc ggcccttcag gagttcggga atggcgcggg
      361 cctggatagg ggttggcttg tcataacctt cagcggcgat cgctttaagg attggcgccg
      421 agaggttgag ttctgaaaat gaattcactg tgcaaatatc tttctgtttc ggacagatga
      481 gtgggaagca cagaacatgt ccgagggctg caacgcgaca atcgcggagt cagcactggc
      541 cctgtgctta aacagacccc acatgaaagg attgttcgga tttttcgctt cggtcggaac
      601 ggcagcatcg aggatgccac ctcatgttat tctttattga ccgaggtcga tggagctgca
      661 tatggacgtt gcagtgcaat aagtcaagcc gcaatcgccg tcaggcagcg cccaagtggc
      721 ggaaagcctt aacaacctgt tcatagacag gcttcttgaa gggcacgatc atacccacga
      781 tctcgtccat cccagcccag cgccactggt cgaattcctg cttgtggccg ggcggcgcca
      841 gattgatttc gctgtcctcg cccaggaagc gcgcggcaaa ccatttctgg gtctggccgc
      901 ggtacttgcc cttccaggac ttgccgatca agtgttcggg cagatcatag ttgtaccacc
      961 cgggagcctc agcgatgatc ttggcggagc gcacggcggt ttcctcttcc agttcacgca
     1021 gtgccgcggc ggcgggatcc tcgctgccat cgatgccgcc ctggggcatt tgccaccagt
     1081 ggccggtgcc ctcgtcattc ttcttttcaa agcgccgccc gatccagaca aggccctgcc
     1141 tgttcaacag catgacgcag acgcacggcc tgtaggccgt aatcactttt gcttcactca
     1201 ttaacccaga cggcccttga acatggcgct gacgggaacc agcagaatgc cacgctcctg
     1261 caactgctcg gcccatagct tcaccgcatc aacggtgact tctagaccgg aaccggtgcc
     1321 gatggcaaag ccattggctt tggcctcgcc ttccaggagt tcaagggcgg cagcgatgct
     1381 ttggacggtg gggtcggcat caatcaccac ctgggcacgg cgctcaggga gcttggtttg
     1441 ctgggcgacg gacttggaga cggtgagcgc ggtggaggca tcttcgagat agacgaggcc
     1501 gcgcttgcgc atttcggcca ttaccggttt gagggcttca ggcgtggcga gcaggcgcgc
     1561 gcccatatag ttggtgatgc cggtatagcc ggcgaagcgg gtcatgtgcc aatgcagggc
     1621 attcaggttt tcctcggctg tgccgccagc caggagggtt ttgggcccgg gattgttggc
     1681 gggatagccc accggttcca tggggagctg caagagcact tcatggccgt cggcgcgggc
     1741 cttgttcacc tgggactgca ggttttcacc atagggcgca aaggcaaagg tcacgtcgcc
     1801 gggcaggttc ttgatggcct gctgggtaag cttggcattg agtcccatgc cgcccagcag
     1861 aatggcaatc ttcggccgtt cggaatgaat gacgcccaag ggcgtgttgc gggcatagac
     1921 atcggaggg
//
LOCUS       DAORVN010000465         1927 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_65433_length_1927_cov_2.401709, whole genome shotgun sequence.
ACCESSION   DAORVN010000465 DAORVN010000000
VERSION     DAORVN010000465.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1927
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65433_length_1927_cov_2.401709"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..536
                     /locus_tag="PKV23_09050"
     CDS             <1..536
                     /locus_tag="PKV23_09050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HQX85622.1"
                     /translation="AGRLDMDSEGLVVLTDDGALQAEIADPRYKLAKRYFAQTEGVPT
                     EAALRWLERGVDLDEFVSKPCTARLMEEPADLWPRTPPIRIRKAIPTSWLELVLKEGK
                     NRQVRRMTAKVGFPTLRLIRWSVGIWNLDGLAPGQWRELEISRQPDVPQRVIRPNAPK
                     NRGVNSSLPIHKRKETP"
     gene            533..784
                     /locus_tag="PKV23_09055"
     CDS             533..784
                     /locus_tag="PKV23_09055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85623.1"
                     /translation="MTKLFAALAAGLIATSAFAADAPKAAPAAAPAAAAPAAAPAAAP
                     AQKGMAGMSGMDATGMAGMEGKAKKAKKAKKAKKADAAK"
     gene            918..1406
                     /locus_tag="PKV23_09060"
     CDS             918..1406
                     /locus_tag="PKV23_09060"
                     /inference="COORDINATES: protein motif:HMM:NF014678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HQX85624.1"
                     /translation="MDGDRHQNVHGVYVMSLRALVLLLLLQPLQWSRAQQPQLPMVEL
                     FAGMHRIEAEVAASPESRAIGLMQRGFMAPQRGMLFVFPEITKHCMWMRNTLLPLSVA
                     FLDEKGAIINVEDMQPKTDDNHCASRPARYALEMNLGWFRSRGFSAGLAISGIEKAGT
                     PR"
     gene            complement(1438..1638)
                     /locus_tag="PKV23_09065"
     CDS             complement(1438..1638)
                     /locus_tag="PKV23_09065"
                     /inference="COORDINATES: protein motif:HMM:NF016028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SlyX family protein"
                     /protein_id="HQX85625.1"
                     /translation="MDSRITDIEVKLSHCEDAIDALNRTVFRQQQQMDLLQAQLRELF
                     RMAQAVGAAESRSLRDEIPPHY"
     gene            complement(1709..>1927)
                     /locus_tag="PKV23_09070"
     CDS             complement(1709..>1927)
                     /locus_tag="PKV23_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HQX85626.1"
                     /translation="GWTWLVKKPDGTVDIVNTSGAGTPLNTENKALLTIDVWEHAYYI
                     DYRNARPKFVETFLASLANWDFAARNFG"
BASE COUNT          391 a          562 c          572 g          402 t
ORIGIN      
        1 ccgccgggcg tctggacatg gattccgaag gtcttgtggt attgactgac gacggtgcgc
       61 tgcaagccga gatcgccgat cccagataca agttggccaa gagatacttc gcgcaaaccg
      121 agggcgtgcc gaccgaggcc gccttgcgat ggctggaacg aggtgttgat ttggacgagt
      181 ttgtcagcaa gccttgcacg gcgaggctga tggaggagcc ggcggacctg tggccgcgga
      241 cgccaccgat tcggattcgg aaagcgattc ccacgagctg gctggaattg gtgctgaagg
      301 aagggaagaa tcggcaggta aggcgcatga ccgcaaaggt cggattcccg acgcttcggt
      361 tgatccgttg gtccgtgggg atttggaatc ttgacggcct tgctcccggt cagtggcggg
      421 agcttgaaat atcgcgtcaa cctgatgtcc cgcagcgcgt tattcggccc aacgccccga
      481 agaatcgtgg tgttaattca tctctaccta tacataaacg aaaggaaaca ccatgaccaa
      541 gcttttcgcc gccctggccg ccggcctgat cgccacctcc gctttcgctg ctgacgctcc
      601 caaggccgct ccggctgccg ctcctgccgc agctgctccc gctgcggccc ccgccgccgc
      661 tccggcccag aagggcatgg ccggcatgtc cggcatggac gccacgggca tggctggcat
      721 ggaaggcaag gccaagaagg ccaagaaggc taagaaggcc aagaaggcag acgccgccaa
      781 gtaattcatg gcaaaaaaga aaatggcagg tttccctgcc attttttttg cccctgcgtt
      841 cccgggagtc gcatattccg acaatcttca ataggacgag tttcatgccc gacttgcgat
      901 gctggtgtcc agcgcagttg gatggcgacc gccaccaaaa cgttcatggt gtgtatgtca
      961 tgtcgttgcg tgcgctagtg ctgctcttgc ttctccagcc cctgcaatgg tcacgagccc
     1021 agcagcctca actgccgatg gtcgagttgt ttgctggcat gcatcgcata gaagcagagg
     1081 ttgccgccag cccggaaagc cgggcaattg gcttgatgca acgagggttc atggcaccgc
     1141 aacgcggcat gcttttcgtt ttccccgaga tcaccaaaca ttgcatgtgg atgcggaata
     1201 cgctactgcc gctctcggtg gcctttctcg acgagaaagg cgccatcatc aatgttgaag
     1261 acatgcagcc gaagaccgac gacaaccatt gcgcctcgcg tcccgctcgc tatgctctgg
     1321 aaatgaatct tggctggttt cgcagccgag gcttctcggc ggggctagcc atcagcggga
     1381 ttgagaaggc cggcacacca cgctgagtct tgcatcgcgt gggccggctt gcggccatca
     1441 gtagtggggt ggaatctcat cgcggaggct acgcgattcg gctgccccaa ctgcttgcgc
     1501 catccggaac aattcgcgca actgagcctg caacaaatcc atttgctgct gctgccggaa
     1561 cacggttcga ttcagtgcat cgattgcatc ctcgcagtga ctgagcttga cttcgatgtc
     1621 cgtgattcgc gagtccatga tcttcctgaa tccgaaaacc aaaggtcgcc attgggcgac
     1681 ctttggcggg cataacagaa gcgcgctctc agccgaaatt cctggcggcg aaatcccagt
     1741 tcgccagcga agccaggaac gtctcgacga acttcggtcg agcattgcgg tagtcaatgt
     1801 aataggcatg ctcccagacg tctatcgtca gcaatgcctt gttctcggtg tttagcggcg
     1861 tgccggcgcc ggaagtgttt acgatgtcca ccgtgccgtc gggctttttc accagccagg
     1921 tccagcc
//
LOCUS       DAORVN010000466         1926 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_65459_length_1926_cov_4.251203, whole genome shotgun sequence.
ACCESSION   DAORVN010000466 DAORVN010000000
VERSION     DAORVN010000466.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1926)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1926)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1926
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65459_length_1926_cov_4.251203"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            334..915
                     /locus_tag="PKV23_09075"
     CDS             334..915
                     /locus_tag="PKV23_09075"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017482740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HQX85627.1"
                     /translation="MAASFPVETTKESDQPLIEQLLDIAFGIERRVKTSYRLREGSTP
                     ADGLSLVVRDAEVGLAGSISFWPLAVGSTGTPALLLGPLVVHPKRQNLGVGLTLMREG
                     LGLAKGYGHRFVILVGDAPYYARVGFQKLPEGQLTLPGSVDPARFLYVELVPGALQGM
                     QGLVLPPFRFAEISVPFAVPHQAERQKQSAQCQ"
     gene            complement(853..>1926)
                     /locus_tag="PKV23_09080"
     CDS             complement(853..>1926)
                     /locus_tag="PKV23_09080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85628.1"
                     /translation="LIAFPTRELFVEKLDQFDLVIFDRYHREAVLPEAYIMQIARYVN
                     EGGALLVAAGPEFARDDGLYSTPMADVLTASPTGEVLDGAFRPKVTRPGTRHPVTGGL
                     PGGEAANPSWGKWFRVVETTAPEIETLMSGLSDKPLLVLSRRGDGRTAQLLSDQSWLW
                     ARGYDGGGPQTELLRRLAHWLMKEPDLEEEALSGRQLGRELTITRRTMADAAEPVTVT
                     APSGAAATVSLTEKTPGLWQGSLKADEAGMYSLNDGKLQAVAATAAGDAREAQDILAT
                     PARLAPVTQATGGGLTWLEDGLPRLIKISGDRQMAGSGWLGLKANGAYRVTAVSEVPL
                     FASLLALGALLLAFCLMWYREGH"
BASE COUNT          325 a          596 c          632 g          373 t
ORIGIN      
        1 caggcgccct gggcgccctc actggagatt tcgcaccggc agtttttcgc gacagaccgg
       61 ctcccggacg gcacgacggg cggaacccgg cggcgcctgg atgaaatcat cgcaaagcaa
      121 gacattggcc accgctggta ggccttgacc tgaatgcccg cgccttgtat ggcggcgtcc
      181 catgatcctt ccggtgacca tatcctcacg acgacgagcc agggctgctg cagccctttc
      241 gcctgcctgg accgcatagg attccttcac ctttattcgt gatcctgtga agtggggccg
      301 ggcccccaac aatcatcatg gaagtcaatc acgatggccg cctcatttcc cgtcgaaacc
      361 acgaaagaat cggatcagcc cctcattgag cagttgctcg atattgcctt tggcatcgag
      421 cgccgggtca aaacctccta ccgtttgcgg gaagggagca cgccggccga tggcctgtcg
      481 ctggtggtcc gcgatgcgga ggttggcctg gcaggatcca tcagcttttg gccgctggcc
      541 gttggcagca cgggaacgcc ggcactgctg ctcggccccc tggtggtgca tcccaaacgg
      601 cagaacctcg gtgtcgggct gaccctcatg cgcgaaggcc tggggctcgc caagggatat
      661 gggcacaggt ttgtgattct ggtgggcgat gcgccctatt atgcgcgcgt tggctttcag
      721 aaactgccgg aaggccagct gaccctgccg ggatccgtcg atccggcacg ctttctttat
      781 gtcgagcttg tccctggtgc tttgcagggg atgcagggac tggtgttgcc gccctttcgc
      841 tttgccgaga tttcagtgcc cttcgcggta ccacatcagg cagaacgcca gaagcaaagc
      901 gcccagtgcc agtagggaag cgaacagggg gacttcgctc acggcggtga cgcggtaggc
      961 cccgttggct ttcaggccga gccagccgga gcccgccatt tgccggtcgc cggagatttt
     1021 gatgaggcgt ggcagaccgt cctcgagcca ggtcaggccg cctccggttg cctgggtgac
     1081 gggcgcgagc ctggctggcg ttgccagaat atcctgcgcc tcgcgcgcat ctccggcagc
     1141 ggttgcggcc acggcctgca gcttgccgtc attgagggaa tacatgccgg cctcatcggc
     1201 cttcaggctg ccctgccaga ggccgggagt tttttcggtc aggctcactg tggccgcagc
     1261 gccggacggc gcggtgacgg tcaccggttc tgccgcatcc gccatggtgc gccgggtgat
     1321 tgtcagttca cggccgagct gcctgccgct cagggcttct tcttccaggt cgggctcctt
     1381 catcagccag tgggcgaggc ggcgcaacag ttcggtttgc gggccgccgc catcatagcc
     1441 cctggcccag agccagcttt ggtcggagag aagctgggcg gtgcggccat cgccgcggcg
     1501 cgacagaacc agcagcggct tgtcggaaag gccggacatg agggtttcga tttcaggtgc
     1561 tgtggtttca acgacacgga accatttacc ccaggaagga ttagccgctt caccgccggg
     1621 caggccaccg gtcacgggat ggcgggttcc aggcctggtc accttggggc gaaacgcccc
     1681 gtcaagaact tcgccggtgg gcgaagctgt cagaacatcg gccatgggcg tgctgtagag
     1741 tccatcgtcc ctggcgaatt caggtcctgc ggccaccagc agggcgccgc cttcgttcac
     1801 atagcgggcg atctgcatga tgtaggcttc gggcagaacc gcctcgcggt gatagcggtc
     1861 aaagatcacc agatcgaact ggtcgagctt ctcgacgaaa agttcgcggg tcgggaaggc
     1921 gatgag
//
LOCUS       DAORVN010000467         1923 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_65644_length_1923_cov_3.379550, whole genome shotgun sequence.
ACCESSION   DAORVN010000467 DAORVN010000000
VERSION     DAORVN010000467.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1923)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1923)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1923
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65644_length_1923_cov_3.379550"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="PKV23_09085"
     CDS             <1..223
                     /locus_tag="PKV23_09085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85629.1"
                     /translation="LVDTTSRKVVASREFNETVIASSETPYAGVVAANLAVQAGLAKL
                     AIFCQEIAQNLRSTAEEAQNPHEKKLQAR"
     gene            complement(279..578)
                     /locus_tag="PKV23_09090"
     CDS             complement(279..578)
                     /locus_tag="PKV23_09090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85630.1"
                     /translation="MKYSVLLSALLVALTLSACDKPTVVTPTVVTVPVPGPAGPTGAT
                     GSTGTAGSTGQTGTTGETGSAGEKGKTGATGEAGKTGGDTIVVVPAPAPEPAPAR"
     gene            900..>1923
                     /locus_tag="PKV23_09095"
     CDS             900..>1923
                     /locus_tag="PKV23_09095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HQX85631.1"
                     /translation="MSESPKNTKQLRRAAEARLEEIAAHKETDGTAFDQAKLIHELQV
                     HQIEMEMQSESLKMAIAALEASHNRYLDLYELSPVGYLTLTPTGEIAEINMTGAQLFG
                     LDRSGLLKRDFSAFVSREDSERWRQFFQSVVRQGGDGKAEFRLVRNDLNLIDLRLDCL
                     AVRREPEEFVIHVAMTDITARMDAEKRLRDKEHLFRVIAERLDGFVAVLDAKGQRVYN
                     SPSYERLLGPRDSIGTNSFLEIHPDDRQRVMQAFSETVASGIGQLLEYRFLLPDGSVR
                     IMESRGGVAWDAVGKTQYVVVISNDVTERKEAERKIHRLAFYDALTKLPNRLTLNDRL
                     QQAMAAS"
BASE COUNT          435 a          559 c          540 g          389 t
ORIGIN      
        1 cctcgtcgac accaccagcc gcaaggtagt cgcctcgcgt gaattcaacg aaacagtcat
       61 tgccagcagc gaaaccccct acgccggggt ggttgcggcc aatcttgcag tgcaggccgg
      121 gctggcgaag ctggccattt tttgccagga aattgcccag aatttgcggt cgaccgcaga
      181 agaagcccaa aacccgcacg aaaaaaaact gcaggcccgt taaagccgca gttttgaggt
      241 tttgcagggc aggtcgcgag cggcctgccc ttcagtgatt agcgcgcagg cgctggttca
      301 ggcgccgggg caggaacgac aacgatggta tcgccgcccg ttttaccggc ttctccggtt
      361 gcgccggtct tgcctttttc accggctgaa ccggtttcgc ccgttgtgcc ggtttgaccg
      421 gttgatcctg ccgttccagt cgagccggtg gcgccggtcg gcccagccgg gcccggcacc
      481 ggcacagtaa ccaccgtcgg cgtgacgacg gtgggtttgt cacaagcgct caaggtcagc
      541 gccaccagta atgcggataa gaggacagaa tatttcatgg aaattccttt gggtaattct
      601 gggagaaatc cctttgtaac cattgattgg tttagctggg gttgaacagt cagcttagac
      661 cttcacgcct gacgcatccg ttcgctagcg cacggacagc ctgagcaaac acccgcggga
      721 accacggctg agcggccggt caaaccgaac cgtcccccga attgatggag attggcgcaa
      781 tgtccataaa tcagggcgcg cttttgcgcg atgccgcaca ttcaaaaata ccgatttaga
      841 ttaatcttcg tgaaaaaatt aaacgacatt atttgaatag cttaccgggg gcagggaaat
      901 tgtcagagtc gccaaagaat actaagcagt tgcgccgcgc ggctgaagca cgtcttgagg
      961 agattgcggc ccacaaagag accgatggca ccgccttcga tcaggcaaaa ctgattcatg
     1021 agctgcaagt ccatcagatc gaaatggaga tgcaaagcga gtcattgaaa atggccattg
     1081 ccgcgctcga agcctcccac aatcgctacc tcgacctcta tgaactttcc ccggtcggct
     1141 acctgacgct caccccaacg ggggaaatcg ccgaaataaa catgaccggt gcccagctct
     1201 tcggccttga tcgaagcggc ttgctcaagc gtgatttttc agcctttgtc agccgcgaag
     1261 acagcgaacg ctggcgccaa ttcttccagt ctgtggtgcg ccaaggcggt gatggcaagg
     1321 cggagttcag gctggtccga aatgatctca acctgattga tctgcgtctt gattgcctgg
     1381 ccgtccggcg tgagcccgaa gaattcgtta tccatgtcgc gatgaccgac atcacggcgc
     1441 gcatggatgc ggaaaaacgc ttgcgcgaca aggaacatct cttccgggtg atcgccgaac
     1501 gtctcgatgg cttcgtcgcg gttcttgatg ccaaggggca acgcgtctac aacagcccat
     1561 cctacgagcg cctgcttggg ccgcgtgaca gcatcggcac gaacagcttt ctggaaattc
     1621 atccggacga ccgccagcgc gtcatgcagg ctttctccga aaccgtggct tcaggtatcg
     1681 ggcaactgct ggaatatcgt tttttactgc cagacggcag tgtccggatc atggaatccc
     1741 gcggcggcgt tgcctgggac gccgtgggga aaacccaata tgtcgtcgtt atctcaaatg
     1801 atgtcaccga gcgcaaagag gccgagcgga aaattcatcg cctggccttc tacgacgccc
     1861 tgaccaaact ccccaaccgc ctgacgctga acgaccgcct gcaacaagcc atggcagcca
     1921 gca
//
LOCUS       DAORVN010000468         1916 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_66055_length_1916_cov_3.680279, whole genome shotgun sequence.
ACCESSION   DAORVN010000468 DAORVN010000000
VERSION     DAORVN010000468.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1916)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1916)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1916
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66055_length_1916_cov_3.680279"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1891
                     /locus_tag="PKV23_09100"
     CDS             <1..1891
                     /locus_tag="PKV23_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HQX85632.1"
                     /translation="GNRQMIASAVPATLIQLVLVYVAFAALTHAYLISDFSVQNVFEN
                     SHSAKPLLYKISGVWGNHEGSMLLWVFILASFGGAVAIFGENLPPTLRARVLAVQGSI
                     GFAFLLFIVVASNPFARLNPVPIEGNGLNPVLQDPALAFHPPFLYAGYVGLSVAFSFA
                     IAALIDGKVDAAWARWVRPWTLAAWMFLTIGISMGSWWAYYELGWGGWWFWDPVENAS
                     FMPWLVATALLHSAIVVEKRNALKVWTVLLSILAFSLSLLGTFLVRSGVLTSVHAFAV
                     DPARGVFILAILCFFIGGALTLFAWRAPALKAGGFFSPISREGGLVLNNLFLCAACAS
                     VLIGTLYPLALEIVTGEKISVGPPYFNLTFGPIMLPLLLLVPIGSQLSWKRADLFAAL
                     QRLWAAAALAIGLGVLVLIATERGPWLAPFGMALGAWLIFGSLADLANRIALFSAPLP
                     TSLSRLAGLPRSSLGMTSAHAGLGLMIIGIIAITLWKVEHIVAMKPGERADIGHYTVQ
                     FVAETPLTGENYTGRAGQFRILRGDSEIATVVSEKREFKPMGTPTTEVGLYQTLAGDV
                     YVVMGDRTADGARVVRLYFNPLVSLIWLGALIMFFGGALSLTDRRYRVGAPKRKLTPA
                     GVPAE"
BASE COUNT          284 a          634 c          536 g          462 t
ORIGIN      
        1 cgggaatcgc cagatgattg cctccgctgt tccggcaacc ctcattcaac tcgtacttgt
       61 ctacgtggcc tttgccgcgc tcacccatgc ctatctgatc tccgatttct ccgtgcagaa
      121 cgtgtttgag aattcccatt cggccaagcc attgctttat aaaatttccg gcgtatgggg
      181 aaaccacgaa ggctccatgc tgctctgggt cttcattctc gcaagcttcg gtggcgccgt
      241 ggcaatcttc ggcgaaaacc tgccgcccac cttgagggcc cgggtgctgg cggtgcaggg
      301 ttccatcggc tttgccttcc ttctctttat cgtggtggcc tccaaccctt ttgcccggct
      361 caatcccgtg cccatcgaag gcaatggcct caacccggtt cttcaggacc cggcgctcgc
      421 ctttcatccg ccgtttcttt acgcgggcta tgtcggcctc tccgttgcgt tctcatttgc
      481 catcgcggca ctgatcgacg gcaaggtgga cgccgcctgg gcccgctggg taagaccctg
      541 gacgctcgcc gcctggatgt ttctcaccat tggcatcagc atgggaagct ggtgggccta
      601 ttatgaactg ggctggggcg gctggtggtt ttgggatccg gtggaaaacg ccagcttcat
      661 gccctggctt gtggcaacgg cgctcctcca ttccgccatc gtcgtggaaa agcgcaacgc
      721 cctcaaggtg tggaccgtgc tgctttccat ccttgccttc tctctatcct tgctcggcac
      781 cttcctggtg cgttccggcg tcctgacttc ggtccacgcc ttcgccgttg atccggcgcg
      841 gggcgttttc attctcgcca tcctctgttt ctttatcggc ggggcgctca cactttttgc
      901 ctggcgtgcg ccggccctga aggcaggcgg attcttctcg cccatcagcc gcgaaggcgg
      961 actcgtgctc aataatctgt ttctctgtgc cgcctgtgcg tcggtcctca tcggaaccct
     1021 gtatccgctg gcgcttgaaa tcgtcaccgg tgaaaaaatc tccgtgggcc cgccttactt
     1081 caatctcacc ttcggaccca tcatgctgcc gctccttctg cttgtcccaa tcggctccca
     1141 gctcagctgg aagagggcgg acctcttcgc cgcgctgcag cggctctggg ccgccgccgc
     1201 actggccata ggcctcggcg tcctggtgct gatcgcaacc gagcgcgggc catggcttgc
     1261 cccgttcggc atggcgctcg gcgcctggct gattttcggc agtctcgccg atttggcaaa
     1321 tcgtattgcc ttgttcagcg ccccattacc caccagcctg tcgcgcctcg caggccttcc
     1381 gcgctccagt cttggcatga cttcagctca tgcgggactc ggcctgatga tcattggcat
     1441 catcgccatc accctgtgga aggtggagca tatcgtggcc atgaaacccg gcgagcgcgc
     1501 tgatattggc cattacacgg tgcagttcgt cgccgaaacg ccgctcaccg gcgaaaacta
     1561 caccggccgc gccgggcaat tccgcatttt gcgcggcgat tctgaaatcg ccaccgttgt
     1621 ttccgagaag cgcgagttca agcccatggg cacgcccaca accgaagtgg ggctctatca
     1681 aacgctcgcc ggcgatgtct atgtggtgat gggcgacagg acggccgacg gcgcccgcgt
     1741 tgtgcgcctc tatttcaatc cgctggtgtc gctgatctgg cttggcgccc tcatcatgtt
     1801 ttttggcggc gcattatcgc tcaccgaccg ccgctaccgg gttggcgcgc caaagcggaa
     1861 actgaccccc gccggagtgc cggcggaatg agatggttcc tgctttgctt cctcct
//
LOCUS       DAORVN010000469         1911 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_66390_length_1911_cov_2.494073, whole genome shotgun sequence.
ACCESSION   DAORVN010000469 DAORVN010000000
VERSION     DAORVN010000469.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1911
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66390_length_1911_cov_2.494073"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..281
                     /locus_tag="PKV23_09105"
     CDS             <1..281
                     /locus_tag="PKV23_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015821188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HQX85633.1"
                     /translation="DNNFVGTELDPKLSYAKIAEACGFKGVTVRTQSETTAALKKAAE
                     DQAKGVTTFIEVILNQELGEPFRRDAMKKPVAIAGISKSDMRLQKGAA"
     gene            310..1344
                     /locus_tag="PKV23_09110"
     CDS             310..1344
                     /locus_tag="PKV23_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012752688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HQX85634.1"
                     /translation="MKAIVTFNAGSSSLKFAVFGSGLDQVLAGQVPGYGPESLQAALV
                     QIESKGILLQHCGGVGHRIVHGGTRYTAPVRVDQKIQDDLESLRKLAPLHLPYGLGAM
                     AALRKLAPDVPHIACFDTAFHAGRDDLHTRLPLPRAYHERGYRRYGFHGLNYEHVVRE
                     LPLQTGKPLPRRLLVAHLGNGSSMCAILDGKSVETTMGFSTADGLLMGTRTGAIDPGV
                     LIALMRDEDLEAEGLEDLLYRRSGLLGISGLSSDMRELLASPAAEAAEAVNAYCYQAA
                     RYAGSLAVAIGGIDAMVFTGGIGENAEPVRQKILAQLSFLGLSAQNVQVVKANEELTI
                     ARHVLSLLRQ"
     gene            complement(1327..>1911)
                     /locus_tag="PKV23_09115"
     CDS             complement(1327..>1911)
                     /locus_tag="PKV23_09115"
                     /inference="COORDINATES: protein motif:HMM:NF013665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HQX85635.1"
                     /translation="AGEMLIGLAPGFVTPSSYFLMQWPERRLIFADCAVNVQPTSEQL
                     ADIAIASAASAARILGEEPRLALLSFSTKGSGTHPDADKVVAAPQIVRQRHPDILIDG
                     ELQGDAALSSAVAARKMAEKGNVAGRANVLIFPDLDAGNIAYKLTQQLSGCRAIGPIL
                     QGFAKPISDLSRGATVEDIVDTASLLLAMSLPKQ"
BASE COUNT          377 a          555 c          615 g          364 t
ORIGIN      
        1 atgacaacaa cttcgtcggc accgagcttg atccgaaact cagctacgca aagatcgcgg
       61 aggcctgcgg cttcaagggc gtgacggttc gcacacaaag cgaaacaacc gccgctctga
      121 agaaggcagc ggaggaccag gcaaaaggcg tcaccacctt catcgaagtc atcctgaacc
      181 aggaactggg cgagcccttc cgccgggatg cgatgaagaa gcctgttgcc attgctggta
      241 tcagcaagtc ggatatgcgc ctgcaaaagg gtgcggcctg atacagaaga atgggataga
      301 accggcagga tgaaagccat cgtcaccttc aatgccggtt cttcctcgct gaagttcgcc
      361 gttttcggaa gcggattgga tcaggtgctt gccggacagg tgccgggcta tgggcccgag
      421 tcgctgcagg cggctctggt tcaaatcgaa agcaagggca ttttgctgca gcattgcggc
      481 ggcgtgggtc accgcattgt gcatggcggc acgcgctata cggcgccggt gcgggttgac
      541 cagaaaatcc aggacgatct tgaatccttg cgcaagcttg cccccctgca ccttccttac
      601 ggtcttgggg cgatggcggc gttgcgaaag ctggcgcccg atgtgccgca tatcgcctgt
      661 ttcgatacgg cgttccatgc gggccgcgac gatcttcaca cgcggctgcc gctgccgcgc
      721 gcctatcacg agcggggtta ccgacgttac ggctttcatg gcctgaatta cgagcatgtg
      781 gtgcgggaat tgccgctgca aaccggaaag ccgctgccgc ggcgcctgct cgtggcccat
      841 ctcggcaacg gctccagcat gtgcgccatc ctcgacggga aaagcgttga aaccaccatg
      901 ggtttttcca cggcggacgg gctgctcatg ggcacccgca ctggcgccat tgaccccggc
      961 gtgttgattg ccctgatgcg tgatgaggac cttgaggcgg aagggcttga agacctgctc
     1021 taccggcgta gcgggctttt gggaatctcg gggctttcgt cggacatgcg cgaattgctg
     1081 gcaagccccg cggcggaggc ggcagaagcc gtcaacgcct attgctacca ggcggcgcgc
     1141 tatgccggat ccctggccgt ggccatcggc ggaattgatg caatggtctt cacaggcggc
     1201 attggcgaga acgccgaacc ggtgcgccag aaaatactgg cgcaactttc attcctggga
     1261 ctctctgcgc agaacgttca agtggtgaag gccaatgagg aactcaccat cgcccggcat
     1321 gtcctatcat tgcttcggca gtgacatcgc cagcagcagg gatgccgtgt ccacgatatc
     1381 ttccaccgtg gcgccacgcg acaggtcgga gatcggtttg gcaaagccct gcaggattgg
     1441 gccgatggcg cggcagccgg agagttgctg ggtgagtttg taggcgatgt tgccggcgtc
     1501 gagatcggga aatatgagca cgttggcgcg gcctgcgacg ttacccttct ccgccatctt
     1561 gcgcgccgca acagcggagg aaagcgctgc gtcaccctga agttcgccat cgatcaggat
     1621 atcgggatgg cgctggcgga cgatctgcgg tgcggccacc accttgtcgg cgtcgggatg
     1681 cgtgccgctg cccttggtcg agaatgagag caaggccagg cgcggttcct cgcccagtat
     1741 tctggcggca cttgctgccg aagcaatggc gatgtcggcc aattgctcgg acgtgggttg
     1801 cacgttcacg gcgcaatcgg caaaaatcag gcgccgctca ggccattgca tgagaaaata
     1861 gctggaaggg gtcacaaatc ccggagccag gccgatcaac atctctcccg c
//
LOCUS       DAORVN010000470         1909 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_66512_length_1909_cov_2.927724, whole genome shotgun sequence.
ACCESSION   DAORVN010000470 DAORVN010000000
VERSION     DAORVN010000470.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1909
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66512_length_1909_cov_2.927724"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..424
                     /locus_tag="PKV23_09120"
     CDS             <1..424
                     /locus_tag="PKV23_09120"
                     /inference="COORDINATES: protein motif:HMM:NF019998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HQX85636.1"
                     /translation="ALYDNPNCREVAEFIGTMNVFSGRTMESAKGAATIDARALGKLQ
                     AQGTSRKGDYVFLAVRPEKLKLMTKKPSAAMNAIGGVVSAISYRGDRHHFLVKAEGHD
                     RPISVSQQNDSETSATPVKVGSKVWVAWPHSAGRLLSN"
     gene            429..1070
                     /locus_tag="PKV23_09125"
     CDS             429..1070
                     /locus_tag="PKV23_09125"
                     /inference="COORDINATES: protein motif:HMM:NF028177.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047869 - dimethylpropiothetin
                     dethiomethylase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfonioproprionate lyase family
                     protein"
                     /protein_id="HQX85637.1"
                     /translation="MQKTVAEAALQKLVAEIKTYVSGLKGPGIASVLAGMDKWASEPS
                     GPIKPRTLPACAHLDAALVAMGKPSLAKAIAEAQPYLHWVSYDAYPREDIGAAFADNH
                     AFASIIGEGCPIEAVDFDLGLFIIAPRLFYRDHHHAAPELYAPLTGPHGWRFKPGDPL
                     DWKKADVPVWNNPWEPHATMTGDIPFLAIFCWTKDTSQPAKIIPSPDWPLFGE"
     gene            1074..>1909
                     /locus_tag="PKV23_09130"
     CDS             1074..>1909
                     /locus_tag="PKV23_09130"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HQX85638.1"
                     /translation="MKTPELIVTNADICTMDPLAPRVAALAVTNGRISALGTVAEIAA
                     LAGPSTRTVDAGGRLVLPGFQDTHIHLQDSGYDHSKSAALHNVSTIAELQKTLADFAA
                     THAGPWVNGVGWYTGIFADHNLNRHVLDAAVPDRPCYIVASDGHNACLNSLGCAAVGL
                     VRGTPDPENGHFVLDGNGEPTGMLHETATYWAESFMPVPTDEDFANGVRYGQALCNRH
                     GITGVIDAMVNERHARVYSSLERAGALTVRVAATAKVVSAEKTEDALRRLEDLRRNAS
                     SDL"
BASE COUNT          387 a          649 c          524 g          349 t
ORIGIN      
        1 agccctttac gacaatccga attgccgcga agtcgccgag ttcatcggca cgatgaatgt
       61 tttttcgggg aggacaatgg aatccgcgaa aggcgcggca acaattgatg caagagccct
      121 tggcaagttg caggcacagg ggaccagccg caagggcgac tacgtcttcc tcgcggtgcg
      181 tcccgagaag ctgaaactca tgacgaaaaa gccttccgcg gcgatgaatg ccataggcgg
      241 ggtggtttca gccatttcct accgtggcga ccgccatcat tttttggtga aggctgaagg
      301 ccacgaccgg cccatttccg tttcccagca gaacgacagc gaaaccagcg ccactccggt
      361 gaaagtgggc agcaaggtgt gggtcgcctg gccccattcc gccggccggc tgctcagtaa
      421 ctaagcccat gcagaaaacc gtcgcagaag ccgctctcca aaaactcgtt gccgagatca
      481 aaacctatgt ctcaggcctg aagggtccgg ggattgcctc ggtccttgcc ggaatggaca
      541 aatgggcaag cgagcctagc ggccccatca agccccgcac acttccggcc tgcgctcatc
      601 ttgatgcggc ccttgttgcc atgggcaagc cttcactggc aaaagccatt gccgaggcgc
      661 agccctatct ccattgggtt tcctacgacg cttacccccg cgaggatatc ggcgcggcct
      721 ttgccgacaa tcacgccttt gcctccatta tcggcgaagg ttgcccaatt gaagcggtgg
      781 attttgacct gggcctgttc atcatcgcgc cgcggctttt ctaccgcgac catcaccacg
      841 ccgctccgga gctctatgcc ccgctcaccg gcccccatgg ctggcgcttc aagccgggcg
      901 atccactgga ttggaaaaaa gccgatgttc ccgtctggaa caacccgtgg gagccccacg
      961 ccaccatgac cggcgacatt ccctttctcg caattttttg ctggaccaaa gatacaagcc
     1021 agcccgcaaa aataatccct tcacccgatt ggcccctttt tggagaatga atcttgaaaa
     1081 cccccgaact catcgtcacc aacgccgata tctgcaccat ggatccactc gcgccacgtg
     1141 tggctgccct cgctgtaacc aatggccgga tctcggcctt gggaacagtc gcggaaatcg
     1201 ccgcactcgc aggcccctca acccgcaccg tagatgccgg cggccgcctc gtgctgcccg
     1261 gctttcagga cacccacatc catctgcagg acagcggcta cgatcacagc aagagtgcgg
     1321 cccttcacaa cgtctcgacc atcgccgaat tgcagaagac cctggccgac tttgccgcaa
     1381 cccatgccgg cccctgggtg aatggcgtcg gctggtatac cggcattttt gccgaccaca
     1441 atctcaaccg ccatgtcctc gacgccgccg ttcctgaccg cccctgctac atcgtggcca
     1501 gcgacggcca caacgcctgc ctgaattccc tgggttgcgc ggcagttggc ctggtgaggg
     1561 gcacgcccga tcccgagaat ggccacttcg tgctcgatgg caatggcgag cccaccggca
     1621 tgctgcacga aaccgcgacc tactgggccg agagcttcat gcccgtgccg accgacgagg
     1681 actttgccaa tggcgtccgc tatggccagg ccctgtgcaa ccgccacggc ataaccggcg
     1741 tcatcgatgc catggtgaac gagcgccacg cccgggtcta cagcagcctg gagcgggcag
     1801 gggccctgac ggttcgcgtc gccgccactg ccaaggtggt ctccgccgaa aaaaccgagg
     1861 atgcgctccg ccgcctcgag gatctgcgcc gcaacgccag ctccgacct
//
LOCUS       DAORVN010000471         1907 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_66631_length_1907_cov_3.552916, whole genome shotgun sequence.
ACCESSION   DAORVN010000471 DAORVN010000000
VERSION     DAORVN010000471.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1907
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66631_length_1907_cov_3.552916"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1907)
                     /gene="gcvP"
                     /locus_tag="PKV23_09135"
     CDS             complement(<1..>1907)
                     /gene="gcvP"
                     /locus_tag="PKV23_09135"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007602217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HQX85639.1"
                     /translation="AMPGRIIGVSIDAEGHRALRMAMQTREQHIRREKATSNICTAQV
                     LLSIIASMFAVYNGPEGIRRMAARTHRLAEIFASAMTSFGYEIATKSFFDTVTIHAPG
                     RAYALFARAKEKRINLRYVDSDHLGISFDQSTRREELKRLLSVFKTDAAERADIDALD
                     KATKETIPKSLQRTSSYLTHPVFEMYHSETEMLRYLRHLQAKDVALDQAMIALGSCTM
                     KLNATTEMIPVTWRNFSLLHPFAPLEQTQGYQQLFEELEAMLAEITGFDAVSLQPNAG
                     SQGEYAGLLAIRGYHAARGQSHRDICLIPISAHGTNPASSVMAGFKVVVVACDEQGNV
                     DVADLKAKAAAHKDNLAALMITYPSTHGVFETAIKDICAIIHSNGGQVYLDGANLNAQ
                     VGVVRPAELGADVCHMNLHKTFCIPHGGGGPGVGPIGVKAHLAPFLPGHSVVDGVNPA
                     AGRDQSQGAVSAAPWGSASILPISWTYIAMMGGEGLRKATIVAILNANYMAKRLNPHF
                     PVLYTGPGGFVAHECIIDLRWLKERTGLGVEDVAKRLVDYGFHAPTMSFPVVDTLMIE
                     PTESECKRELDRFCDAMIEIRKEIAEVEEGRADRLDNVLKNAPHTHRLLLGDWKHPYS
                     KEKAFFPLKGY"
BASE COUNT          363 a          514 c          635 g          395 t
ORIGIN      
        1 catagccctt gagcgggaag aaggcctttt ccttggaata ggggtgcttc cagtcgccga
       61 gcagcagccg gtgcgtatgc ggggcgtttt tcagcacatt gtcgagcctg tcggccctgc
      121 cctcctccac ctcggcgatt tccttgcgga tttcgatcat ggcatcgcag aaacggtcga
      181 gctcgcgctt gcactcggat tcagtgggct cgatcatcag ggtatcgact accgggaaag
      241 acatggtggg ggcatgaaag ccgtaatcca ccaggcgctt ggccacatcc tcgacgccaa
      301 ggccggtgcg ttccttcagc cagcgcaggt caatgatgca ttcatgggcc acaaagccgc
      361 cgggcccggt gtagagcacg gggaaatggg gattaaggcg tttggccatg tagttggcgt
      421 tgaggattgc gacaatggtc gctttcctga ggccctcacc gcccatcatg gcgatgtagg
      481 tccaggagat cggcaggatg gaagcggagc cccagggggc ggccgatacc gcaccctgcg
      541 actggtcgcg gcctgcggcg gggtttacgc catcaaccac gctatggccc ggcaggaagg
      601 gagcgagatg ggccttcacg ccgatggggc cgacgccagg cccgcccccg ccgtgcggta
      661 tgcagaaagt cttgtgcagg ttcatgtggc agacgtcggc gccgagttcg gcgggccgca
      721 cgacgcccac ctgggcatta agattggcgc cgtcgagata gacctggccg ccgttggaat
      781 ggatgattgc gcagatatcc ttgatggcgg tttcgaaaac gccgtgggtt gagggatagg
      841 tgatcatcag ggcggcgaga ttgtccttgt gcgccgcggc cttggctttg agatcggcca
      901 catcgacatt gccctgctca tcgcaggcca ccaccacgac cttgaatccg gccatcaccg
      961 atgaggcggg attggtgccg tgcgccgaga taggaatgag gcaaatgtca cggtggctct
     1021 gcccacgcgc ggcatggtag ccgcggatgg ccagcaggcc cgcatattcg ccctggctgc
     1081 cggcgttggg ctgcagcgac acggcatcga aaccggtaat ttccgcgagc atggcttcga
     1141 gttcttcgaa gagctgctga tagccctgcg tctgttccaa tggtgcaaag ggatggagca
     1201 gcgaaaagtt gcgccaggtc accggaatca tttcagtggt cgcgttgagt ttcatggtgc
     1261 aggaccctag cgcaatcatc gcttgatcca atgctacatc ttttgcctgc aagtggcgga
     1321 gatacctcaa catttctgtt tcagagtgat acatctcgaa gaccggatgg gtgaggtaac
     1381 ttgaggtgcg ctgcaatgat tttggaatgg tctctttcgt ggccttgtcc agcgcatcga
     1441 tatcggcgcg ctctgccgca tcggtcttga agacgctgag gaggcgcttc aattcttcgc
     1501 ggcgcgtgga ctgatcaaag ctgatgccga gatgatcgga atcaacatag cggaggttga
     1561 tgcgtttttc cttggcgcgg gcaaagagcg cataggcgcg gcccggcgcg tggatggtca
     1621 ccgtatcgaa gaacgacttg gtggcgattt catagccgaa tgaggtcatg gcgctggcaa
     1681 aaatttcggc aaggcgatgg gtccgcgccg ccattctgcg gatgccttcc gggccgttgt
     1741 aaacggcgaa catggaggcg atgatggaaa ggaggacctg ggcggtacag atattgctgg
     1801 tggctttctc acggcggata tgctgctcgc gggtttgcat ggccatgcgg agcgcgcggt
     1861 ggccttcggc gtcgatggac acgccaatga tgcggcccgg catggcg
//
LOCUS       DAORVN010000472         1900 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_67108_length_1900_cov_3.105691, whole genome shotgun sequence.
ACCESSION   DAORVN010000472 DAORVN010000000
VERSION     DAORVN010000472.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1900)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1900)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1900
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67108_length_1900_cov_3.105691"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(24..1826)
                     /gene="lepA"
                     /locus_tag="PKV23_09140"
     CDS             complement(24..1826)
                     /gene="lepA"
                     /locus_tag="PKV23_09140"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015596601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HQX85640.1"
                     /translation="MTDTAHIRNFSIIAHIDHGKSTLADRLIQLCGAVSDRDMVDQIL
                     DSMDLERERGITIKANTVRLDYTARNGEKYVLNLIDTPGHVDFAYEVSRSLAACEGSL
                     LVVDASQGVEAQTLANVYQAINNNHEIIPVLNKVDLPAAEPERVRQQIEDVIGIDASE
                     AVMISAKTGLGVPEVLEAIVHRLPPPKGDRNKPLKALLVDSWYDSYLGVVVLLRIIDG
                     ELKKGMRVKMMGTGGSYLIDRVGVSRPKKEMVASLGPGEIGFFTAAIKEVADTRVGDT
                     VTDEKRPTAEALPDFKEAQAVVFAGFFPVDAAKFEDLREAMGRLRLNDASFSFDMETS
                     AALGFGFRCGFLGLLHLEITRERIEREFNVDIITTAPSVIYKINMRDGTQKSLHNPVD
                     MPDAALIESIEEPWIEATILVPDEYLGSILKLCEDRRGRQKQLTYAGSRAMVIYELPL
                     NEVVFDFYDRLKSVSRGYASFDYKMVGYEKGDLVRMGILVNAEPVDALAMVVHRARAE
                     QRGRVMCEKLKDLIPRHMFQIPIQATIGGKIVARETLSAMRKDVTAKCYGGDISRKRK
                     LLDKQKEGKKKMRQFGKVDIPQEAFIAALKMDEN"
BASE COUNT          351 a          593 c          553 g          403 t
ORIGIN      
        1 acttgcgcgt cgatgggcgc gccttagttc tcgtccatct tcagcgccgc gatgaaggct
       61 tcctgcggaa tgtcgacctt gccgaactgc cgcatcttct tcttgccttc cttctgcttg
      121 tccagaagct tgcgcttgcg gctgatgtcg ccgccgtagc atttcgccgt cacgtccttg
      181 cgcatggctg aaagcgtctc gcgcgccacg atcttgccgc cgatggtggc ttggataggg
      241 atctggaaca tgtggcgcgg aatgagatcc ttgagcttct cgcacatcac ccggccgcgc
      301 tgctcggccc tggcccggtg caccaccatg gccagcgcat cgacaggctc cgcattcacc
      361 aggatgccca tgcgcaccag gtcgcccttt tcataaccca ccatcttgta atcgaagctg
      421 gcatagccgc ggctcactga tttgagccgg tcatagaaat caaaaaccac ttcgttgagc
      481 ggcagctcat agatcaccat ggcgcggctg cccgcatagg tgagctgctt ctgccgcccg
      541 cgccggtcct cgcacagctt gaggatggag cccagatatt catcgggcac caggatggtg
      601 gcttcgatcc acggctcctc gatggattca atcagcgcgg cgtccggcat atcaaccgga
      661 ttgtgcagcg atttctgcgt cccgtcgcgc atgtttatct tgtagatgac gcttggcgcc
      721 gtggtgatga tgtccacgtt gaattcgcgc tcgatgcgtt cgcgggtgat ctcgagatga
      781 aggaggccga gaaagccgca gcggaaaccg aaacccagcg ccgccgaggt ttccatgtcg
      841 aaggaaaagc tggcgtcatt gaggcgcaag cgccccatcg cttcgcgcag gtcttcgaac
      901 ttcgccgcat cgacgggaaa gaaaccggca aacaccacgg cctgcgcttc cttgaagtcg
      961 ggcagggctt ccgccgtcgg ccgcttttca tcggtcaccg tatcgccgac gcgcgtatcc
     1021 gccacttcct tgatcgccgc cgtgaagaag ccgatctcgc cggggccaag gcttgccacc
     1081 atttcctttt tcgggcgcga gacgccgacg cggtcaatga ggtaggagcc gcccgtgccc
     1141 atcatcttga cccgcatgcc cttcttgagc tcgccgtcaa tgatgcgcag cagaaccaca
     1201 acgcccagat aggaatcata ccagctatcg accagcagtg ccttgagcgg cttgttgcgg
     1261 tcgcccttgg gcggcggaag gcggtgcacg atggcctcaa gcacctccgg aacgccgagc
     1321 ccggtcttgg ccgagatcat caccgcctcg gaggcgtcaa tgccgatcac atcctcgatc
     1381 tgctgtcgca cccgctccgg ctcggccgcg ggaagatcaa ccttgttgag aaccgggatg
     1441 atttcatgat tgttgttgat cgcctgataa acgttggcca gggtctgcgc ctccacgccc
     1501 tggctggcat caaccaccag aagcgaaccc tcgcaagccg ccagcgaacg cgaaacctca
     1561 taggcaaagt ccacatgccc cggcgtatcg attaggttca gaacatattt ttcgccgttc
     1621 ctggcggtgt agtcgaggcg caccgtgttg gccttgatgg tgatgccgcg ctcccgctcc
     1681 aggtccatgg aatcgaggat ctggtctacc atgtcccggt cgctcaccgc cccgcaaagc
     1741 tggataagcc ggtcggcaag cgtcgatttg ccgtggtcga tatgggcgat aatggagaaa
     1801 ttgcggatat gggcggtgtc agtcattgcg cgcggtgttt ggcattggca tggccaatgg
     1861 tcaagacttg caatcagcgc agtttccggg gttttaccgg
//
LOCUS       DAORVN010000473         1899 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_67162_length_1899_cov_3.809111, whole genome shotgun sequence.
ACCESSION   DAORVN010000473 DAORVN010000000
VERSION     DAORVN010000473.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1899)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1899)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1899
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67162_length_1899_cov_3.809111"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..512
                     /locus_tag="PKV23_09145"
     CDS             <1..512
                     /locus_tag="PKV23_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008126147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HQX85641.1"
                     /translation="TLVNNAGVSVQNRGDLLDVTAESYDRCLNVNTRGTFFLTQAFAR
                     RLRPSQAHRSIVTISSANASGVSVNRGEYCISKAGVSMMSKLFAARLGNEGVGVYEIQ
                     PGFIETDMTAPSKARYDKLIDEGLTVIKRFGTPEEVGRIALTLAQGLLPYTTGQIIQA
                     DAGLMMVRY"
     gene            515..1654
                     /locus_tag="PKV23_09150"
     CDS             515..1654
                     /locus_tag="PKV23_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003527048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HQX85642.1"
                     /translation="MKIRLPAAEGKSETYHLAGEPIAVRKPKTPFNRVTLAAAHVVAD
                     PLSPSGGLDWDKTLAFRHFLLDQGFGIAEAMDTSQRGMGLDWPLAHELIGRSLKSAGP
                     QASRIFSGCGTDHLPPVEARSVDDVVKAYLEQLHAIQKLGGRVILMASRALVRVAKSP
                     DDYVKVYGRVLNEAEKPVILHWLGEMFDPALKGYWGAEDFSATARTCLAVISNNSAKI
                     DGIKISLLDAEKEIIFRRQLPANVKMYTGDDFNYADLIAGDTQGYSHALLGIFAAIAP
                     AAAAALTALAKGNTKKFHKIFGPTVPLSRLIFQAPTQYYKTGIVFLAWLNGHQDHFIM
                     VGGAQSARPLPYFTETFKLADQAGLLRDPELAVKRMKTLLALYGQ"
     gene            1651..>1899
                     /locus_tag="PKV23_09155"
     CDS             1651..>1899
                     /locus_tag="PKV23_09155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HQX85643.1"
                     /translation="MMALNSGQCAVNTATLGFQCPIEQSIDAIAAAGFGGIAPWRREV
                     EDQDVPVIAKRIRDAGLKVTGYCRSTYLPSPTREGLQAN"
BASE COUNT          391 a          536 c          583 g          389 t
ORIGIN      
        1 ccacgctggt caacaacgcc ggtgtttcag tgcagaaccg cggcgatctt ctggatgtca
       61 cggcggaaag ctatgaccgc tgcctgaatg tgaacacgcg gggtactttc ttcctgaccc
      121 aggccttcgc gcgccgcctc cgtccttcgc aagcgcatcg cagcattgtg acaatatcat
      181 cggcaaatgc cagcggggtt tcggtcaacc ggggtgaata ctgcatctcg aaggcgggcg
      241 tttcgatgat gtcaaaactg tttgcggcgc gcctcggcaa cgagggcgtc ggtgtctatg
      301 agattcagcc gggcttcatc gagacggata tgacagcgcc ttcgaaggca cggtatgaca
      361 agctgattga cgagggattg acggtgatca agcgatttgg aacgccggaa gaagtgggcc
      421 ggattgcgct caccctggcg caggggctgc tgccttacac aaccgggcag atcatccagg
      481 cggatgcggg actcatgatg gtgcggtact gaacatgaaa atacgtttgc cggcagcgga
      541 aggaaaatcc gagacttatc atctggcggg cgagcccatc gccgtccgga agcccaaaac
      601 cccatttaac cgcgtcaccc ttgcggccgc ccatgtggtg gctgatcccc tgagcccctc
      661 cggcgggctg gactgggaca aaacgctggc attccgccac tttcttctcg accagggttt
      721 tggcatcgcg gaagccatgg atacttcgca gcgcggcatg ggactggatt ggccgctggc
      781 ccatgaactg atcggacggt cactcaaaag cgccgggcct caagcctcgc ggatattctc
      841 cggctgcggc acggatcatc tgccgccagt ggaggcacgt tcggtcgacg atgtggtgaa
      901 ggcctatctc gaacagcttc atgccattca aaagctcggc ggccgggtga tcctgatggc
      961 aagccgggcg ctggtgcggg tggcgaaatc ccccgatgat tatgtgaagg tttatggccg
     1021 cgtgctgaac gaggccgaga aacccgtaat cctgcactgg ctgggcgaaa tgttcgatcc
     1081 agcgctcaag ggctattggg gggcagagga tttttccgct acggccagga cctgtcttgc
     1141 ggtgatcagc aataactccg ccaagattga cggcatcaag atttcgctcc tcgatgcgga
     1201 gaaggagatc attttccgcc ggcagcttcc agccaatgtc aaaatgtata cgggcgacga
     1261 ctttaattac gctgacctga ttgccggcga cacacagggc tactcgcatg cgctgctcgg
     1321 tattttcgcg gccatagcac ctgcggcggc ggcggcgtta accgcgctgg ccaagggcaa
     1381 cacaaagaag tttcacaaga tttttggccc cacggtgccg ctgtcacggc tgatctttca
     1441 ggcacccacc caatactaca agacgggcat tgtttttctg gcgtggctca acgggcatca
     1501 ggaccatttc atcatggtgg gcggggcgca aagcgcgcgg ccgcttccct actttaccga
     1561 aacattcaag ctggccgatc aagcaggact gctccgtgat cccgagcttg cggtgaagcg
     1621 gatgaaaacc ctgcttgcgt tgtatggcca atgatggcgc tgaattccgg ccaatgtgcg
     1681 gtcaatacgg caacgctggg tttccagtgc cccattgagc aatccattga tgcgattgcc
     1741 gctgcgggtt ttggcggcat agcgccgtgg cggcgcgaag ttgaggacca ggatgttcct
     1801 gtcattgcaa aacggattcg cgatgcgggc ctgaaggtca cgggctattg ccgcagcacc
     1861 tacctgccgt caccgacgcg ggaaggtttg caggcgaac
//
LOCUS       DAORVN010000474         1898 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_67223_length_1898_cov_3.950624, whole genome shotgun sequence.
ACCESSION   DAORVN010000474 DAORVN010000000
VERSION     DAORVN010000474.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1898)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1898)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1898
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67223_length_1898_cov_3.950624"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="PKV23_09160"
     CDS             complement(<1..294)
                     /locus_tag="PKV23_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018495498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HQX85644.1"
                     /translation="MSLSDLTTLTAAYGFTERNDPLQLFADWLADAEKSEPNDPNGMA
                     LATVDEEGLPNVRMVLLKDADERGFVFYTNYESQKGQEILGTMKAALCFHWKSL"
     gene            433..891
                     /locus_tag="PKV23_09165"
     CDS             433..891
                     /locus_tag="PKV23_09165"
                     /inference="COORDINATES: protein motif:HMM:NF028308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RT0821/Lpp0805 family surface protein"
                     /protein_id="HQX85645.1"
                     /translation="MIKTYAAVAMAGFMLAGCAQDAGPKETSGALIGGLAGGLLGNTM
                     GHGKGRAAATVLGAALGAVVGGKVGRSLDDEDRNYAYGAATRSFSTGREITWENRESG
                     HRGRFRPKRSFERDGGLCRDFEHTIWVEGEPDVIEGTACQTPDGRWRVVN"
     gene            complement(955..>1898)
                     /locus_tag="PKV23_09170"
     CDS             complement(955..>1898)
                     /locus_tag="PKV23_09170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85646.1"
                     /translation="SFSVELADAETGYITWAKRYDATGQDLLSWAGEVCPMIVAALDP
                     ALAESEHQALRKPTLAATDCLIAYQYLVIGYRHYHAAQWQQAIAAFQNAVNEDATYAH
                     AHAMISVNRYMDAQISRNPGFDGELEKAEHSARRALEIDPSEAKANIGLGQIMDWRGR
                     HSESMGYLERAVVLNPSFAHASTARSYHAVMTGSFESAKTHIQNAMRLRVGDAGLGFC
                     LPSKALADLHLGNFKDALQTAHWAMRLTPDFWLTRLVLAASLHAAGDFESAASLVHDL
                     KRDYAGLNADEFAAWFPYANPRDGVAITKSLVQSGWH"
BASE COUNT          395 a          529 c          614 g          360 t
ORIGIN      
        1 cagcgacttc cagtggaaac agagggcagc cttcatggtg cccagaattt cctggccctt
       61 ctggctttca tagttcgtgt aaaaaacaaa gccgcgctca tcggcgtctt tcaaaaggac
      121 catgcggaca ttgggcaggc cctcctcatc aacggtggca agcgccatgc cgttgggatc
      181 attgggttcg gacttctccg cgtcagccag ccagtcggca aaaagctgca gcggatcgtt
      241 gcgctcggta aaaccgtagg cggcggtcag ggtcgtcagg tcgctcaggc tcatggcatt
      301 aaccaatcat tcgaggggtc caatgtagcc ccagactctt cacatttcat cccacttcgg
      361 ccacgggaag cgctcatttg ctccctagat ggttaaagca atatgggacg caagtcgcgg
      421 ggaattcagc ggatgatcaa gacctatgca gcagttgcaa tggcaggctt catgcttgcc
      481 ggatgcgccc aggacgccgg accgaaggaa acttccggcg cgttgatcgg cggccttgcc
      541 ggaggcctgc tcggcaacac catgggccat ggcaagggcc gcgcggcagc aaccgttctc
      601 ggcgcggcgc ttggcgctgt cgtgggcggc aaggttggcc ggagccttga tgacgaagac
      661 cgcaattacg cctatggcgc ggcgacccgt tcattcagca cggggcggga aatcacctgg
      721 gaaaaccgtg aaagcgggca tcgcggacgc ttccggccaa agcgcagctt tgagcgcgac
      781 ggcggactat gccgggattt cgagcatacc atctgggtgg agggcgaacc ggatgtcatt
      841 gaaggcaccg cctgccagac gccggacggg cgctggcgtg tggtgaactg actgaaaata
      901 ctaatccgac aaatctggtg cctgatccga accaatcact gtcgtgacgc agcctcaatg
      961 ccagcccgac tgcacaaggg acttggtgat cgcaacgcca tcgcggggat ttgcgtaggg
     1021 aaaccacgcg gcgaattcat cggcgttgag gccggcgtaa tcgcgcttca gatcatgcac
     1081 caggcttgcg gcggattcga aatcgccggc ggcatgcagg cttgcggcga ggacaaggcg
     1141 agtcaaccag aagtcggggg tgaggcgcat ggcccaatgg gcggtttgca gcgcgtcctt
     1201 gaaatttccc aggtggagat cagcgagggc ctttgacggc aggcaaaaac ccagtcccgc
     1261 atcacccacc cgcaggcgca tggcgttttg aatatgggtt tttgccgatt caaaggaacc
     1321 ggtcatcact gcatggtagc tgcgcgccgt cgaggcatgg gcaaaacttg gattgaggac
     1381 aacggcgcgc tcgagatagc ccatggactc actgtggcgt ccacgccagt ccatgatctg
     1441 gccgaggcca atgttggcct tggcttctga agggtcgatt tccagggcgc ggcgggcgct
     1501 gtgctcggcc ttttccaatt caccgtcgaa gccagggttg cgcgaaatct gggcgtccat
     1561 gtagcggttc accgaaatca tggcgtgggc atgggcgtag gtggcgtcct cgttcacggc
     1621 gttctggaat gcggctatgg cctgctgcca ttgcgccgcg tgataatgac gatagccaat
     1681 gacgagatac tgataggcaa tcaggcagtc ggttgcggca agggtgggct tgcgcagggc
     1741 ctggtgctca ctttcggcaa gggcgggatc gagggccgcg acaatcatcg ggcaaacttc
     1801 gccggcccag gacaaaagat cctgaccggt ggcgtcataa cgcttggccc aagtgatgta
     1861 gcccgtctcg gcatcggcga gttcgacgga gaacgaga
//
LOCUS       DAORVN010000475         1890 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_67735_length_1890_cov_2.942779, whole genome shotgun sequence.
ACCESSION   DAORVN010000475 DAORVN010000000
VERSION     DAORVN010000475.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1890
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67735_length_1890_cov_2.942779"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..941)
                     /locus_tag="PKV23_09175"
     CDS             complement(<1..941)
                     /locus_tag="PKV23_09175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85647.1"
                     /translation="MAVNLLGVRLGGAAIGLLSQILLARLLSQHDVGVVFLGMSAAAF
                     ISLLITGGYPILAITCLPRYYALGRKNLVHAFHAAFLRDSLWISLTSFALAAVAYFWL
                     PLNDGLKIALLFGCLSAPASALIRMNGSIANSVRRYALSYVPDFLYRPGLLLAYLVFA
                     WAVGFHLSIAHVLWAFAITNTIVALAQAWLIGEDGARSGLKWSGRHNMAPVLRGRAAA
                     LVIVGIVTASFADVVTLIAGFFLNTADVAVVGVTIRLAALAGFITQATQQFILPDLAA
                     AMTRGTSQQVHALLLRINMIALAAILAGVVGALLLGP"
     gene            complement(1042..1563)
                     /locus_tag="PKV23_09180"
     CDS             complement(1042..1563)
                     /locus_tag="PKV23_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018239345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HQX85648.1"
                     /translation="MEKSPEAFRTISEAAEELDVPQHVLRFWETRFAQIKPMKRAGGR
                     RYYRPADLDLLKGIRSLLYSDGYTIRGLQRILKEEGVAYVSGVGRGEIVARKRDMELD
                     GVPRPGQTQIAPRFRGSAPLPGRAPLARHGSSVGEAPVLVHKLSDMQADALKAALRDL
                     GDARKLLDPTSRI"
     gene            complement(1595..>1890)
                     /locus_tag="PKV23_09185"
     CDS             complement(1595..>1890)
                     /locus_tag="PKV23_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018390992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HQX85649.1"
                     /translation="TRADLSEAVHRDIGLSRTESAEMVKSVLDLGSTALVEGTSVKLS
                     SFGTFMVRHKKGRIGRNPKTGEEVPITPRRVMVFRPSQVMKNIINGLEPGSDD"
BASE COUNT          448 a          547 c          569 g          326 t
ORIGIN      
        1 cagggaccca gcagcagcgc gcccacgaca ccggccaata tagcggcaag cgcgatcatg
       61 ttaatacgca agagaagagc gtgaacctgt tgggaggtgc cccgcgtcat ggccgcggcc
      121 aggtcaggca ggatgaactg ctgggttgcc tgggtgatga agcccgccag ggctgccagc
      181 ctgatggtga caccgacaac ggccacatcc gccgtgttca ggaaaaagcc cgcgataagg
      241 gtgacaacgt cggcaaagga agccgtaaca atgccaacaa tgaccagcgc tgccgcccgg
      301 ccgcgcaata cgggcgccat attgtgccgg cccgaccatt tgaggcctga gcgtgctcca
      361 tcctcgccaa tgagccaggc ctgggcaagg gcaacaatcg tgttcgtgat cgcgaacgcc
      421 cacagcacat gggcaatgct caagtgaaac cccacggccc aggcgaagac cagataagcc
      481 agcaagagcc cgggacgata gagaaaatct ggaacataag acagcgcata gcgcctgaca
      541 gaattggcga tggatccgtt catgcggatg agcgccgaag ccggagctga aaggcagccg
      601 aagagcaatg cgatcttcaa gccatcgtta agcggcagcc agaaataggc cacggcagca
      661 agtgcgaacg aggtgagcga aatccacaag ctgtcgcgta ggaatgcggc gtggaaggcg
      721 tgaacgagat tttttctccc cagcgcgtaa tagcggggca aacaggttat cgccaggatc
      781 gggtagccgc cggtgatcag gaggctgatg aaggcggcgg cactcatgcc cagaaagacg
      841 acacccacat catgctggga gaggagccgg gccagcagaa tctggcttaa aaggccaatg
      901 gcggcgccgc ccaatctcac accgaggaga ttgaccgcca taagaattgt tcgccaaagg
      961 gacatgccta gggtgccttt tccggacttg aaaggaatag caataaacag gcggtcttta
     1021 ttttgtattg cgaagaaccg gtcaaatccg cgaggtggga tccagcagtt tccgggcatc
     1081 gcccaggtca cgcagggcgg ccttcaaggc atcggcctgc atgtcggaca gcttgtgcac
     1141 caagaccggg gcctcgccga cgctgctgcc atggcgggcc agaggggccc ttccgggcag
     1201 cggggccgag ccgcgaaagc gcggggcgat ctgggtctgg ccaggccgtg gcacgccgtc
     1261 cagttccatg tcacgcttgc gggcaacgat ttcaccgcgg cccacgccgg agacataagc
     1321 aacaccctct tccttcagga tgcgctgcag tccgcgaatg gtgtagccgt cgctatagag
     1381 aaggcttcgg atgcctttca gcagatcgag gtcggcgggg cggtaatagc gccggccgcc
     1441 agcccgcttc atgggcttaa tctgggcaaa acgggtttcc cagaacctca gaacatgctg
     1501 gggaacatca agctcttctg ccgcttcgct aatggtgcgg aatgcttcag gtgacttttc
     1561 caaggactat ctccgattcg ccgtaaagag atactcaatc atcactgccc ggctcaagtc
     1621 catttatgat gttcttcatc acctggcttg ggcggaaaac cattacgcgg cgcggcgtaa
     1681 tgggcacctc ttcaccagtc tttgggttgc gcccgatgcg gcctttcttg tggcgtacca
     1741 tgaacgtgcc aaaggaagaa agcttaaccg acgtaccttc caccagggcg gttgaaccca
     1801 gatccaaaac agacttcacc atttcggccg attcggtgcg cgacaatcca atatcccgat
     1861 gcaccgcttc acttaagtcc gcacgtgtca
//
LOCUS       DAORVN010000476         1889 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_67798_length_1889_cov_2.944929, whole genome shotgun sequence.
ACCESSION   DAORVN010000476 DAORVN010000000
VERSION     DAORVN010000476.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1889
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67798_length_1889_cov_2.944929"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            32..1255
                     /gene="serS"
                     /locus_tag="PKV23_09190"
     CDS             32..1255
                     /gene="serS"
                     /locus_tag="PKV23_09190"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006072610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HQX85650.1"
                     /translation="MRGLAAQSAALLKIDSEKRDHIQKLQDAQARRNALSKEIGEVMR
                     AGDKAKAETLKAEVAGLKDFIAAGEEAERALTQKLNEALSVIPNLPMDGVPKGKDEHD
                     NKELHTWGEKPKFNFEPRQHFDIGEWLGLMDFETAAKLSGARFVILKGQLAKLERALG
                     LFMVDLHTEHHGYTEIYAPYMLRDEAMFGVGQLPKFKEDLFQTTSGHWLIPTAEASLT
                     NIVRERVLEEKELPIRMTAYTPCFRAEAGAAGKDTRGMIRQHQFSKVELVSITTPEQA
                     EAEHSRMLACAEEVLKRLKLAFRTIVLCTGDMGFGSRKTYDIEVWLPGQNAYREISSC
                     SQLGEFQARRMEARFKPADGKGPRYVHTLNGSGLAVGRTMVAILENYQNDDGSVTIPE
                     ALRPYMGGAEKITRA"
     gene            complement(1271..>1889)
                     /locus_tag="PKV23_09195"
     CDS             complement(1271..>1889)
                     /locus_tag="PKV23_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012558617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HQX85651.1"
                     /translation="YHIQSDLTHAELAAVPCAFATAENLVSRAGLNAGESILITGASG
                     NVGIAAIQLAKRRGAHVTVIAARGKFKELQAAGAARCVERGSAIAAAVGNNSMDVVVD
                     VVGGPQWPELLDVLKPRGRYAISGAIAGPIVHLDLRKLYLKDLTLFGCTSQDEQVFPN
                     LVRYLESGELKPIVARQYPLRDIAVAQEEFLAKKHVGKIVLVPPE"
BASE COUNT          417 a          554 c          559 g          359 t
ORIGIN      
        1 taaccccgca gcctttgatc agggccttgc cttgcgcggc cttgccgcac aatcggcggc
       61 gcttctcaag attgattcgg aaaagcgcga ccatatccag aagctgcagg acgcgcaggc
      121 ccgccgcaac gcgctgtcga aagaaatcgg cgaggtgatg cgggcagggg acaaggcaaa
      181 ggccgaaacc ctcaaggcgg aggttgccgg cctcaaggat ttcatcgccg ctggcgagga
      241 agccgagcgc gctctgactc aaaagctcaa tgaggcgctt tcggtgatcc cgaaccttcc
      301 catggacggc gtaccgaagg gcaaggacga gcacgacaac aaggagctcc acacatgggg
      361 tgaaaagccc aagttcaatt tcgaaccgcg ccagcatttc gacatcggtg aatggctggg
      421 gctgatggat tttgaaacgg cggccaaatt gtcgggcgcg cgttttgtca ttctcaaggg
      481 ccagcttgcc aagctggagc gggcgcttgg cctgtttatg gtcgatctgc atacggagca
      541 tcacggctac acggaaatct atgcgccata catgctgcgc gacgaggcga tgttcggcgt
      601 cggccagctg ccgaaattca aggaggacct gttccagacc acgtcgggcc actggctcat
      661 tcccacagcg gaagcctcgc tcaccaacat cgtgcgcgag cgcgtgctcg aagaaaaaga
      721 gcttcccatc cgcatgacgg cctacacgcc gtgtttccgc gctgaggcag gagccgcagg
      781 caaggatacg cggggcatga tccgccagca ccagttctcg aaggtggagc ttgtttccat
      841 caccacaccg gagcaggcgg aagccgaaca cagccgcatg ctggcttgcg ccgaggaagt
      901 gttgaagcgc ctgaagcttg ccttccgcac catcgttttg tgcacgggcg acatgggttt
      961 cggcagccgc aagacctatg acatagaagt gtggctgccc gggcagaacg cctaccgcga
     1021 aatctcgtcg tgttcgcagc ttggcgaatt ccaggcgcgg cgcatggagg cgcgcttcaa
     1081 gccggcggac ggcaaaggcc cgcgttatgt tcatacgctg aacggatcag ggcttgccgt
     1141 gggccgcacc atggttgcca ttctggaaaa ctatcagaat gacgacggca gcgtgaccat
     1201 tccggaagcg ctccggccct acatgggcgg agcggaaaaa atcacgcgcg cctgatccgc
     1261 tgccaaccgg tcattccggc ggaacgagca caatcttccc aacatgtttt tttgccagaa
     1321 attcttcctg ggcaacggca atgtcacgta gtggatattg cctggcgaca atgggcttta
     1381 actcaccgct ttcgaggtaa cgaacaagat tcgggaatac ttgctcatcc tgtgaagtgc
     1441 atccaaacaa ggtcaggtcc ttgagataga gcttgcgcaa atccaaatgg actatcggcc
     1501 cggcaattgc gcccgatatg gcatagcggc cccgtggctt taggacatca agcagttccg
     1561 gccactgcgg cccgccaaca acatcaacga caacatccat gctgttgttt cccacggccg
     1621 ccgcaattgc cgagcctctc tcgacgcaac gggcggcacc tgccgcctgc agttccttga
     1681 actttccgcg agcagcgatg accgtaacat gggccccgcg ccgttttgcc aattgaatgg
     1741 cggcgatccc tacgtttccc gatgctccgg tgatgaggat actttctccg gcattcagtc
     1801 ctgcacggct gacaagattt tcggcggtgg caaatgcgca aggcacggcg gcaagctctg
     1861 catgggtgag atcagattga atgtggtag
//
LOCUS       DAORVN010000477         1886 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_67994_length_1886_cov_2.657018, whole genome shotgun sequence.
ACCESSION   DAORVN010000477 DAORVN010000000
VERSION     DAORVN010000477.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1886
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67994_length_1886_cov_2.657018"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="PKV23_09200"
     CDS             <1..209
                     /locus_tag="PKV23_09200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85652.1"
                     /translation="LNSETIANITILLPPLAQQKEVMAYLDDKIAKFDTLTAEAQHAI
                     DLLQERRSALISAAVTGQIDVRPR"
     gene            279..542
                     /locus_tag="PKV23_09205"
     CDS             279..542
                     /locus_tag="PKV23_09205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85653.1"
                     /translation="MSSISANDLKTKGISAIEAALSDAPEAIVSVRGKDKFVVMDLAH
                     YHYLRECELDAALAETRADLAAGRFTKESVDEHLAWLDQACSN"
     gene            530..802
                     /locus_tag="PKV23_09210"
     CDS             530..802
                     /locus_tag="PKV23_09210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85654.1"
                     /translation="MFQLIFTQQFKRKEIQFLKRHPELRQPYAKTLRLLEANPAHPSL
                     RLHPLRGKHEGLHSVSINLSYRITLELLIQDQQIIPVNVGDHDAVY"
     gene            884..1189
                     /locus_tag="PKV23_09215"
     CDS             884..1189
                     /locus_tag="PKV23_09215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85655.1"
                     /translation="MKTTLDLPDEIVREMKLRAVVQGRTLKELVADLLRQGLGMGQRT
                     PLERPPAGSMVEIGANGLPVIRCRADAPASRMPVEALLQLEQQAQTEEDLRHAGIAV"
     gene            1170..1619
                     /locus_tag="PKV23_09220"
     CDS             1170..1619
                     /locus_tag="PKV23_09220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HQX85656.1"
                     /translation="MPGSLFDTNVWVAAIFPSHPFHLTAQQVLQLATPTEPAVFCRST
                     QQSFLRLASTPTLLKAYGAEGLTNRDALLALSALLALPQVCEREEPPGTLLLWHRLAA
                     HDTASPKVWMDAYLAAFAIGSSLRIVTLDRDFKNFVPQGLDLNLLNP"
     gene            1682..>1886
                     /locus_tag="PKV23_09225"
     CDS             1682..>1886
                     /locus_tag="PKV23_09225"
                     /inference="COORDINATES: protein motif:HMM:TIGR01552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HQX85657.1"
                     /translation="MITVTSVEAQNRFGQLLDTVQREPVVITRHGRMVAVLMTPQAAE
                     AQRQGVGADAARQAGLAAIANFRG"
BASE COUNT          399 a          578 c          525 g          384 t
ORIGIN      
        1 gattgaatag cgagaccatc gcgaacatca caatcctcct ccctcctctt gcacaacaaa
       61 aggaagtgat ggcttatctc gacgacaaaa tcgccaaatt cgacaccctc accgccgaag
      121 cccaacacgc catcgatctg ctgcaagaac gccgcagcgc gctgatctcc gccgccgtca
      181 ccggccagat cgacgtgcgt ccgcgctgat cgcttgcttt atcgcacgat tgatggcact
      241 ctttggcgca cgcattaacc attgttcagg agcttcccat gtccagcatc agcgccaacg
      301 atcttaaaac caagggcatc tcggccatcg aggctgctct cagcgatgcg ccggaagcca
      361 tcgtctcggt gcgcggcaag gataaattcg tggtgatgga tttggcgcat taccactatc
      421 tgcgcgagtg cgagctggat gccgcgctgg cggaaacacg cgccgacctg gcggctggcc
      481 gattcacgaa agaatccgtg gacgagcatc tggcctggct tgatcaagca tgttccaact
      541 gatttttacg cagcaattca aacgcaagga aatccagttt ctaaaacggc atcccgagct
      601 caggcagccc tacgccaaga cattgcgact tctggaagcc aaccccgccc atccctcgct
      661 gcgcctgcac cctttgcgcg gcaagcatga aggcctgcat tcggtgtcga tcaatctgtc
      721 ctaccgcatc acgctggaac tgttgattca ggatcaacaa atcatcccgg tgaatgtggg
      781 tgaccatgac gcggtttatt gatcgctaca gtcgtcaccg acaggaaagt cggcgctggc
      841 ggaacggcga cacagcaagc cagcaagctg gtaagcttgc cacatgaaaa ccacacttga
      901 cctacccgat gagatcgttc gggaaatgaa attgcgtgcc gtcgttcagg ggcgcactct
      961 caaggaactt gtcgccgact tgctccggca ggggctaggc atgggacagc gcacgccatt
     1021 ggagagacct cccgccggtt ccatggtcga aatcggcgct aacggcttgc cggtaattcg
     1081 ctgccgtgcc gatgcccccg ccagccgcat gcctgtcgag gccttgctcc agcttgaaca
     1141 gcaagcccag accgaggagg acttgcggca tgccgggatc gctgtttgat accaatgtat
     1201 gggttgcggc gatcttcccc agccatccat ttcacctgac agcccaacaa gtccttcagc
     1261 tggcaacgcc cacggaaccg gcggtttttt gccgttcgac acagcaaagt tttctgcgtc
     1321 tggcctccac gcccacgcta ttgaaagcct acggcgccga gggcttgacc aatcgcgatg
     1381 ccttgctggc actgtcggcc ttgctggctt taccgcaagt ttgcgaacgg gaagagccgc
     1441 cgggtacgct tctcctctgg catcggctgg cggcgcatga cacggcctcc cccaaagtct
     1501 ggatggatgc ctacctcgcc gctttcgcaa tcggcagtag tttgcgcatc gttacgctgg
     1561 accgggattt caagaacttc gtgccgcagg ggctcgattt gaacttgctc aatccctgat
     1621 agaaaagggc tggattctgg ctatactgta caggttagct attatttcaa ggagcacatt
     1681 catgattacc gttacctcgg tggaagccca gaatcgtttt ggccagttgc tcgacacggt
     1741 gcagcgcgag ccggtcgtca ttacccggca cgggcgcatg gtggcggtgt tgatgacgcc
     1801 gcaggcggct gaagcgcagc ggcagggcgt gggcgccgac gcagccagac aggcggggct
     1861 cgccgccatc gcaaactttc gcgggg
//
LOCUS       DAORVN010000478         1885 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_68052_length_1885_cov_3.175410, whole genome shotgun sequence.
ACCESSION   DAORVN010000478 DAORVN010000000
VERSION     DAORVN010000478.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1885)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1885)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1885
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68052_length_1885_cov_3.175410"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..777)
                     /locus_tag="PKV23_09230"
     CDS             complement(<1..777)
                     /locus_tag="PKV23_09230"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HQX85658.1"
                     /translation="MDQKLLPFALQDVPRYTSYPTAVQFQDGFPGDQADRWLAELPPD
                     ASLSIYVHVPFCQQLCWYCGCHTSVPNSYGRASAYVDSLLSDITRSGKLSGAEKGNVK
                     HLHFGGGTPTYLSNADLGRILACINGSFGLAPEAEIALESDPRGITRDRAFGLAAMGF
                     NRISFGVQDFSLPVQMKINRLQTHGLVAHVAELLREAGFTSINFDLMYGLPAQTAGSV
                     RQTAKQAAALRPSRISVFGYAHVPWFKKHQKSISTDELPSS"
     gene            complement(782..1567)
                     /locus_tag="PKV23_09235"
     CDS             complement(782..1567)
                     /locus_tag="PKV23_09235"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HQX85659.1"
                     /translation="MIHLADCNSKLPDSDRTVSPILKHLLGVSSLAELESGERQRLEG
                     ARWLVRSIGKNCSLIDQGEDREVVGVLLSGWAFRYQTLPDGKRQILDFIFAGSLVGFG
                     SGATNSYGVEMVTACTVATLPHAQFRRLLATCPTLAMQVVERVSNSEMRAHEHMTSLG
                     RRSARERIAALIVELTCRAQCTDLGKREYSIDLPVTQIMIGDALGLSNEHVCRVLGKL
                     AGDGVIEINNHTLKVLDQAALAREAGTEVRLPVPRQGSLPIAA"
BASE COUNT          375 a          540 c          619 g          351 t
ORIGIN      
        1 gctcgaagga agctcgtcgg tgctgatcga tttctggtgc ttcttgaacc agggcacgtg
       61 ggcatagccg aatacggaga tgcggcttgg cctgagggct gccgcctgtt tcgccgtctg
      121 gcgtacgctg cccgcggttt gcgcgggaag cccgtacatc aggtcaaaat tgatggaagt
      181 gaagccggct tcacgcaaaa gttcggccac atgggctaca aggccatggg tctgcaggcg
      241 gttgatcttc atctgcaccg gaagggaaaa atcctgcacg ccgaaactga tgcggttgaa
      301 acccatcgcg gcaagcccga aggcacggtc gcgggtgatg ccgcgggggt ccgactcaag
      361 ggcaatctcg gcttccggcg cgaggccgaa acttccgttg atgcaggcca gaatgcgccc
      421 gagatccgcg ttgctcagat aggtcggcgt gccgccgccg aaatggagat gcttgacatt
      481 gcccttttcg gctccgctca atttgccgct gcgggtgata tcgctcagca gactgtcgac
      541 ataggcgctg gccctcccgt aggaattggg caccgaggtg tggcagccgc aataccagca
      601 gagctgctgg caaaacggca catgcacata gattgaaagc gaagcatcgg gcggcagttc
      661 agccagccag cggtcggcct ggtctcccgg aaagccatcc tggaactgga cagcggtggg
      721 atagctcgtg tagcgcggca catcctgcag ggcgaaaggc agaagcttct ggtccatggc
      781 ctcacgcggc gattggcaat gagccttggc gggggacggg gagacggacc tcggttcccg
      841 cttcccgggc aagggctgcc tggtcgagaa ccttcagggt atggttgttg atctcgatca
      901 cgccatcgcc cgccagtttg cccagcaccc ggcagacatg ctcattggac aggccaagcg
      961 catcgccgat catgatctgg gtaacgggaa ggtcaatgct gtattcgcgt ttccccaggt
     1021 cggtgcactg ggcccggcag gtgagttcga cgatcagcgc ggcaatgcgc tcgcgggcgc
     1081 tgcgccggcc aaggctggtc atgtgttcat gggcgcgcat ctcgctgttt gaaacgcgct
     1141 caacgacctg catcgccagg gtggggcagg ttgccagcag gcgacggaat tgtgcgtggg
     1201 gcagggtggc gacggtgcag gctgtcacca tttccacacc gtaggaattg gtggcgccgg
     1261 aaccaaagcc caccagcgaa ccggcaaaaa tgaagtcgag aatctggcgc ttgccatcgg
     1321 gcagggtctg atagcggaag gcccagccgg agagcagcac gcccaccact tcgcggtctt
     1381 cgccctggtc gatcagcgag cagttcttgc cgatgctccg caccagccag cgcgcgcctt
     1441 caagccgctg ccgctcaccg ctctcaagct cggcgaggga ggaaacgccc agcaggtgct
     1501 tgagaatggg cgaaacggtt ctgtcagaat cgggaagctt gctgttgcag tctgcgaggt
     1561 ggatcatcgt ctggctccct tttgcggctc ccggctcaaa gccaggcgtg tcgactgttg
     1621 ggaggatgaa acgacggttt ttgcccggaa cctagacggg aatagtacgt aggtaatttt
     1681 actcagtcca aatcgggcat tggccggcga acagcacagg cgaatcagcc tgcgttccgc
     1741 cccagggatt cagctttggc aagccaggat tggcgcttgt cctgggacac ggcttcaatc
     1801 atgccatgaa tcgtcgcacg tactggcgaa acgccgacga agccaagaat gttgcgcttc
     1861 agcatgcgca gggcgaaggc gccga
//
LOCUS       DAORVN010000479         1882 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_68239_length_1882_cov_2.928298, whole genome shotgun sequence.
ACCESSION   DAORVN010000479 DAORVN010000000
VERSION     DAORVN010000479.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1882
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68239_length_1882_cov_2.928298"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1005
                     /locus_tag="PKV23_09240"
     CDS             <1..1005
                     /locus_tag="PKV23_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014331397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQX85660.1"
                     /translation="LYPVLALLASVIVCAALVMLSGASPLSVFGLMVKGAAGTTFAFL
                     ETLTRATPLIFTGLAAAVAFRAKLWNIGGEAQLYIGAVMTVVLGTGFLPLPQPILIPV
                     LMLAAMLGGAMVLSIPTFLKTRFGVDEVVTTLLLNFIAILFVSMLLEGVLKDPTGLGW
                     PQSKKVIAEAQLPKLIQGKRLHFGFVVAIAAAIAVWIIDRQSTLGYEMRAVGHSPKAA
                     SFAGIPVNRVLAKTALLSGGLAGLAGMSEVTGLKGNLTLDLSPGFGYTGIVVAMLALM
                     HPLGVIAAAIFVAAVFVGADAMSRTAGVPSYIAHVMVATSLLTMVSAIMLTRYRVRWR
                     "
     gene            1008..>1882
                     /locus_tag="PKV23_09245"
     CDS             1008..>1882
                     /locus_tag="PKV23_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018426360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQX85661.1"
                     /translation="MEAVEILFTASLWAAAIRIASPLIFGTLGELICERAGVLNLGIE
                     GIMTAGAFAGWLTVFLGGDLWLGVAVAAMVGALFGLLHGFLVVPLGLSQHVTGIGITL
                     LATSLSYYFYRLIFPSVTSPPKIEPFQPWPIPFVSDIPVIGEALFRQTPLTYLAFVAV
                     LLVAWLLYKTPIGLAIRTVGENPSAAEAQGVDVTLVRMGAVMLGSALMGIGGSFITLS
                     AFNSFFFEMMNGRGWICVALVVFGSWRPGKALAGALLFGAFDAYQLRLQQLAGAYVPY
                     QVFLMLPFILSIIAL"
BASE COUNT          283 a          572 c          535 g          492 t
ORIGIN      
        1 ctctatcccg tgctcgccct tttggcttcg gtgattgttt gcgcggctct cgtcatgctc
       61 tccggggctt ccccgctttc agtgtttgga ttgatggtga agggggcggc gggaacgaca
      121 ttcgcgtttc tcgaaaccct cacccgggcg acgccattga ttttcacggg tcttgcagcg
      181 gccgtggctt tccgcgccaa gctttggaac attggcgggg aggcgcaact ctatatcggt
      241 gccgtaatga cagttgttct gggcacaggt ttcttgccgt tgccgcaacc cattctcatt
      301 cccgttctga tgctggctgc catgctgggc ggcgccatgg ttctttccat accaacattc
      361 cttaagacac gtttcggggt tgacgaagtc gttaccaccc tgctcctcaa tttcatcgcc
      421 atattgtttg tcagcatgct tctggaaggc gttttgaagg atccgaccgg tctgggctgg
      481 ccccagtcga aaaaggtcat cgccgaggcc cagttgccaa agctaattca gggcaagcgc
      541 ctgcattttg gctttgttgt cgccattgcc gctgccatcg cggtctggat catcgaccgg
      601 cagtcgaccc tgggctatga aatgcgtgcc gtgggccaca gtcccaaggc ggcctcgttt
      661 gccggcatcc cggtcaatcg cgtgctggca aaaaccgccc tgctctccgg cggcctggcg
      721 ggccttgccg gcatgtcgga ggtcaccggc ctcaagggca acctcacgct cgatctatcc
      781 ccgggatttg gctacacagg aatcgttgtg gcgatgctgg cactcatgca tcccttggga
      841 gtgattgccg ccgccatttt tgtcgctgcg gtttttgtcg gcgccgatgc catgagcagg
      901 acggcaggcg ttcccagcta catcgcccat gtaatggtgg caacctccct cctcaccatg
      961 gtttccgcca tcatgctcac gcgctaccgc gtcaggtgga gatgaacatg gaagcggtag
     1021 aaatcctgtt caccgcatcg ctctgggccg cggccattcg tatcgcctcg ccgctcattt
     1081 tcggaaccct cggcgaactc atctgcgagc gggccggggt tctgaatctc ggtattgaag
     1141 gtatcatgac ggcgggagcc tttgccggct ggctcaccgt attcctgggt ggggacctgt
     1201 ggctcggtgt tgcggttgct gccatggtgg gagccctctt cggtctgctg catggttttc
     1261 tggtggttcc gctcggcctc tcgcaacatg tgacgggtat tggcataacc ttgcttgcca
     1321 catcgctctc ctattatttt taccgtctga tctttccgtc ggtcacctcg ccgcccaaga
     1381 ttgagccctt ccagccctgg cctattccct tcgtgtcgga tattccggtt atcggcgaag
     1441 ccctgttcag gcaaacacca cttacctacc tggcctttgt cgccgttctc ctcgtggcct
     1501 ggctccttta caagacgccc atcggccttg ccatccgcac cgtcggggaa aatccctcgg
     1561 ccgctgaagc gcagggggtc gatgtcaccc tcgtgcgcat gggcgcggtc atgctgggaa
     1621 gtgcccttat gggcatcggc ggaagtttca ttacactctc cgccttcaat tcattcttct
     1681 ttgaaatgat gaacgggcgc ggctggattt gcgtggcgtt ggtggtgttt gggtcatggc
     1741 gcccgggcaa ggctttggct ggcgcccttc tgtttggcgc ttttgatgcc taccagcttc
     1801 gcctgcagca gctggcaggg gcctatgttc cctatcaggt gtttctgatg ctacccttca
     1861 tcctgtccat catcgcactc at
//
LOCUS       DAORVN010000480         1877 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_68551_length_1877_cov_3.037322, whole genome shotgun sequence.
ACCESSION   DAORVN010000480 DAORVN010000000
VERSION     DAORVN010000480.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1877)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1877)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1877
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68551_length_1877_cov_3.037322"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..84
                     /locus_tag="PKV23_09250"
     CDS             <1..84
                     /locus_tag="PKV23_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009363652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HQX85662.1"
                     /translation="IRTLDPGEYSFFDDFQPGSPPAILTAK"
     gene            93..935
                     /locus_tag="PKV23_09255"
     CDS             93..935
                     /locus_tag="PKV23_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011949941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family iron permease"
                     /protein_id="HQX85663.1"
                     /translation="MTPNPGIIFVIWRESTEALLIIGILNAWLTHRPSLERVAAQRWL
                     WCGAGAGLLGAVGLAAVLIQVGDALSDEAQEYFQMGVVFVAAALIVQMIFWMRRNGRT
                     IQADLHGALEGAARKSKWFAVFCLAALAVLREGSETAIFLYGTMAGIPGGNFAPMLSV
                     ALGLLAGLGTYWLLQVSGRIFSWRFFFRVTEVMLLFLAASLVMAGLDHLVSLGMLPSP
                     SVGLWDTSQFLPDSGMFGSMIAGLTGYRAKPLLVEVCVYAAYWVLILWFLNRPRAGVR
                     SRYA"
     gene            932..>1877
                     /locus_tag="PKV23_09260"
     CDS             932..>1877
                     /locus_tag="PKV23_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002966223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HQX85664.1"
                     /translation="MSRSASPNLPVARIGEWLRDHQQAIRRLQWAVVVFYVLLLVVPA
                     LVPMPDRQSHIWSNVVLFAQFIFWGIWWPFVLLSMVLVGRSWCGLFCPEGALAEAASR
                     FGLGRATPGWIKWKGWPFTAFACTTIYGQMVSVYQYPAPALVVLGGSTLAAIAVGLRM
                     GRGKRVWCRYLCPVSGVFGLLSKLAPLHYRVDQAAWRGWGSEAGRRAPAVNCAPLVPI
                     RTMRGASACHMCGRCAGFKGAVALEVRSSSREIVEVAGADPRPWETVLIVFGLMGLAA
                     GAFQWTASPLYVMAKLAIAEWLIDHGATMLMEPRWPWWI"
BASE COUNT          294 a          540 c          595 g          448 t
ORIGIN      
        1 atccgcacac tcgatcctgg tgaatattcg tttttcgatg atttccagcc cgggtccccg
       61 ccggcaatac tgactgcgaa atagcgccgc gcatgacacc aaaccctggc atcatatttg
      121 tcatctggcg ggagagcacc gaagcgctgc tgatcatcgg gattctcaac gcctggctca
      181 ctcatcgccc atcgctggag cgcgtggccg cgcagcgatg gctttggtgt ggagcaggtg
      241 ccggcttgct cggagcagtt ggccttgcgg ctgttttgat acaggtgggc gatgcgttaa
      301 gcgatgaggc acaggagtat ttccagatgg gtgtcgtatt cgtggcggcg gcgcttatcg
      361 ttcagatgat attctggatg cggcgaaatg gccgcaccat acaagccgac cttcatggcg
      421 cgcttgaagg agccgccagg aaatcaaagt ggttcgccgt cttctgcctg gctgcgcttg
      481 ctgttcttcg tgaaggcagc gaaacggcga ttttccttta cggaaccatg gctggcattc
      541 ctggcggcaa ctttgctcca atgctgtctg tggcactcgg tttgctggcg gggctcggca
      601 cctattggct tctccaggtc agtggccgaa tcttctcctg gcggtttttc ttccgcgtca
      661 cggaagtcat gctgctgttc cttgcggctt cgcttgtcat ggccgggctg gaccatcttg
      721 tttcgcttgg catgctgccc tcgccgtcag ttggcctgtg ggacacatcg caattcctgc
      781 ccgacagcgg gatgttcggc agcatgattg ccggccttac gggttaccgt gcgaagcctc
      841 tcctggttga agtttgtgta tacgccgcct actgggtgct catcctatgg tttctcaacc
      901 gtccacgcgc aggcgtcaga tcccgatacg catgagccgc tccgccagtc ccaacttgcc
      961 cgtcgcgcgt atcggcgaat ggctgcgcga ccatcagcaa gcgatacgac gcttgcagtg
     1021 ggctgtggtc gttttctatg tgctcctgct ggtagttccc gccctggtgc cgatgccaga
     1081 ccggcaatcg cacatctggt ccaacgtggt gctctttgcc cagttcatat tctggggaat
     1141 ttggtggccg ttcgtcctgt tgagcatggt gctggttggg cgctcttggt gcgggctgtt
     1201 ctgcccggaa ggggcgctcg cggaagcggc aagccggttc ggcctcggcc gggcaacacc
     1261 gggatggatc aagtggaagg gatggccgtt cacagcattc gcctgcacca cgatctatgg
     1321 ccagatggtg agtgtctatc aatatccagc gccagccctc gtcgttctcg gaggctccac
     1381 cctggcggcc atcgcggtgg gcttgagaat ggggcgcggg aagcgcgttt ggtgccggta
     1441 tctttgtcct gtcagcggcg tgtttggcct cctgtcaaaa ctcgcgccgt tgcactatcg
     1501 cgtggatcag gcagcgtggc gcggctgggg aagcgaagcc ggtcggcggg caccggcagt
     1561 caattgcgcc cctcttgtgc ccattcgcac catgcgcggc gcttccgcct gccacatgtg
     1621 cggccgctgc gcaggattca agggcgcagt cgcattggaa gtgcgctcat catcacgcga
     1681 aatcgttgag gtcgcggggg ctgatcccag gccttgggaa accgttctca tcgtcttcgg
     1741 actcatgggg cttgccgcgg gcgcctttca gtggacggca agccctttgt atgtcatggc
     1801 gaaactggcg attgcggagt ggcttatcga tcatggcgca accatgctga tggagccgcg
     1861 gtggccttgg tggatac
//
LOCUS       DAORVN010000481         1877 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_68558_length_1877_cov_2.817234, whole genome shotgun sequence.
ACCESSION   DAORVN010000481 DAORVN010000000
VERSION     DAORVN010000481.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1877)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1877)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1877
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68558_length_1877_cov_2.817234"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..546
                     /locus_tag="PKV23_09265"
     CDS             <1..546
                     /locus_tag="PKV23_09265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HQX85665.1"
                     /translation="WVAENGVRRNAKGEALSAEFLIESPIFERVLGPYVKNLKLIGID
                     ASIRIVDDAQYQSRRKSFNYDILSSRFGSDVTPGTGLRVFFGTASADAPGSFNVSGVK
                     SPAIDSLIEQVISSNSRKELDIAGRALDRVLRAEHFWVPNWHKASHWIAHWDRYGRPA
                     IKPKYDRGIVDTWWYDTAKGN"
     gene            555..1661
                     /locus_tag="PKV23_09270"
     CDS             555..1661
                     /locus_tag="PKV23_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012046865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HQX85666.1"
                     /translation="MGAYILKRILLMVPTLLGIMLMTFTVIQFVPGGPIEQVIAKMQG
                     TDTDATARISGNAGNEAGGNEQSQLGGDAGIKYKGAQGLDPEFIKSLEKQFGFDRPVH
                     ERFLKMIGDYATFDFGRSYFRDRTVIDLIVEKMPVSISLGLWLTLITYLVSIPLGIRK
                     AVHAGSNFDVWTSAVIIVGYAIPSFILAILLIVLFAGGSYYSWFPLRGLTSDNFDQLS
                     WFGKIADYAWHMALPLFALGIGGFATMTLLTKNSFLDEIRKQYVMTARAKGLTERQVL
                     YGHVFRNAMLLVISGFPGAFLGAFFAGSLLIETVFSLDGLGYLTYKAALDRDYPIVFA
                     NLYIFTLVGLVVALISDLTYMLIDPRIDFESREV"
     gene            1665..>1877
                     /locus_tag="PKV23_09275"
     CDS             1665..>1877
                     /locus_tag="PKV23_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008596158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQX85667.1"
                     /translation="MALFTLKGLNRRRWELFKANRRGLWSLWIFGALFLMSVFAPIIA
                     NDRPMLVSYKGELLFPTFVNYHKSKFG"
BASE COUNT          360 a          555 c          541 g          421 t
ORIGIN      
        1 tgggttgcag aaaatggtgt gcgcagaaac gccaagggcg aagccctttc ggctgaattc
       61 ctcattgaga gcccgatctt cgaacgggtt ctcgggccct atgtgaagaa cctgaagctc
      121 atcggaatcg acgccagcat ccgcattgtc gacgacgcgc aataccagtc acggcgcaag
      181 agtttcaatt atgacattct ctccagccgc ttcggctccg acgtgacgcc ggggaccggg
      241 ctccgggttt tctttggaac cgcttctgcc gatgcgccgg gaagtttcaa cgtttccggg
      301 gtcaagagcc ccgccatcga ctcgctgatc gagcaggtga tctcctccaa cagccgcaag
      361 gaacttgata tcgccgggcg ggcgcttgac cgggttctgc gcgccgagca tttctgggtg
      421 cccaactggc acaaggccag ccactggatt gcccattggg accgctacgg gcggcctgcg
      481 atcaagccca aatatgaccg tggcattgtc gatacctggt ggtatgacac ggccaaagga
      541 aactgaatcg caccatgggc gcctatatct tgaaacgcat actgctgatg gttccgacgc
      601 ttctcggcat catgctgatg accttcaccg tcatccagtt cgtgccgggc gggcccatcg
      661 agcaggtgat cgcgaagatg cagggcaccg acacggatgc cacggcgcgg atcagcggca
      721 atgcgggcaa cgaggcaggc ggcaacgagc aatcgcaact gggcggggat gccggaatca
      781 aatacaaggg ggcgcagggg ctggacccgg aattcatcaa gtcgctggag aagcagttcg
      841 gcttcgacag gccggtgcat gaacgcttcc tgaagatgat cggcgactac gcgaccttcg
      901 atttcggccg gagctatttc cgtgaccgga cggtcatcga cctgatcgtc gagaaaatgc
      961 cggtttccat ttcgctcggc ctttggctga cgctcatcac ctatctggtt tcgattccgc
     1021 tcggcatccg caaggcggtg catgcgggat ctaatttcga cgtgtggact tcggcagtga
     1081 tcatcgtggg ctatgcgatc cccagcttca tcctggcgat cctgctcatc gtcctgttcg
     1141 ccggcggcag ctattacagc tggtttccgc tgcgcggcct gacgtctgac aatttcgacc
     1201 agttgagctg gttcggaaaa atcgccgact atgcctggca catggcgctg ccgctgtttg
     1261 ccctcggcat cggcggcttt gccaccatga cgctactcac caagaattcc ttcctcgacg
     1321 aaatcaggaa acagtatgtg atgacggcgc gggccaaggg cctgaccgag cgccaggtgc
     1381 tttatggcca tgtgttccgc aatgccatgc tgctggtgat ttccggcttc cccggcgcct
     1441 tcctcggtgc cttctttgcc ggatcgcttc tgattgaaac ggtgttctcg ctcgatggcc
     1501 tgggatacct cacctacaag gcagcacttg accgcgatta ccccatcgtc ttcgccaacc
     1561 tctacatctt cacccttgtg ggccttgtgg tggccctgat cagcgatctc acctacatgt
     1621 tgatcgatcc gcggatcgat tttgaatcac gcgaggtcta gggcatggcg ctctttaccc
     1681 tcaaaggact gaaccgccga cgctgggaac tgttcaaggc caaccgccgg gggctttggt
     1741 cgctatggat tttcggggcg cttttcctga tgagcgtgtt tgcgccgatc attgccaatg
     1801 accggcccat gctggtgtcc tacaagggtg agctgctgtt cccgaccttt gtcaattatc
     1861 acaaatccaa gttcggc
//
LOCUS       DAORVN010000482         1872 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_68846_length_1872_cov_3.148046, whole genome shotgun sequence.
ACCESSION   DAORVN010000482 DAORVN010000000
VERSION     DAORVN010000482.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1872)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1872)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1872
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68846_length_1872_cov_3.148046"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="PKV23_09280"
     CDS             <1..267
                     /locus_tag="PKV23_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017269602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein"
                     /protein_id="HQX85668.1"
                     /translation="GRDPDLLIVGQPTRGVDIGAIEFIHRRIIALRDEGKAILLVSVE
                     LDEIRSLSDRILVMFAGRIMGERGPEANERELGLLMAGVSKDAA"
     gene            267..1361
                     /locus_tag="PKV23_09285"
     CDS             267..1361
                     /locus_tag="PKV23_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019963426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQX85669.1"
                     /translation="MAARGELPVWVDLALIPLLNLLFALVISGLVVLIIGENPLEAMW
                     IMLKGAVGSLKGWGYLLYYATNFVFTGLAVAVAFHAGLFNIGGEGQAYMAGAGAAAVG
                     LSLEFLPWPIVIPLSILASMAVGAFWAWVPGVLQVKRGSHIVITTIMFNYIALSLMAY
                     LINYGFRPVGKMAVESREITGAYIMSFREVAREFFDLRLPSTPLNFSVFLAIAAAFAV
                     WYLLWRTKLGYEIRAVGASPEAAVYAGIRPDRIIVITMAISGALAGLMAVNEILGAQH
                     RLVLGFVQGAGFVGIAVALMGRSHPLGIMLSAMLFGLLYQGGTELSFAKPDINRDMIV
                     VIQGLVILFMGALEYAFRPWLTALFFRPED"
     gene            1367..1840
                     /locus_tag="PKV23_09290"
     CDS             1367..1840
                     /locus_tag="PKV23_09290"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85670.1"
                     /translation="MELYVEILASTIRYTVPLILACIAGLWSERSGIVDIGLEGKMLI
                     AAFVAASGAAVYGSAWIGLGLAILTSVGFALIHGYAAINQRGNQVVSGVAINMLAAGL
                     AVVLGNEWFREGGRTPALPPEGRFLPLEIPGISALADVPGLGAVVKIFFSHSILT"
BASE COUNT          276 a          614 c          542 g          440 t
ORIGIN      
        1 ggccgcgatc ccgatttgct gatcgtcggc cagcccaccc gcggcgttga tatcggcgcc
       61 attgaattca tccacaggcg tatcattgcc ctgcgcgacg aaggcaaggc catcctgctg
      121 gtctcggtgg agcttgatga aatccgctcg ctctccgacc gcattctggt gatgtttgcc
      181 ggacgcatca tgggcgagcg cggccccgaa gccaatgagc gcgagcttgg cctgctcatg
      241 gccggcgtct cgaaggatgc agcctgatgg cggcgcgcgg tgaacttccg gtctgggttg
      301 acctggcgct gattcctctc ctgaacctct tgttcgcttt ggtgatttcc ggacttgtgg
      361 tgctcatcat cggtgaaaat cctctggaag ccatgtggat catgctgaaa ggcgccgtgg
      421 gctcgctcaa gggctggggc tatctcctct actacgctac aaacttcgtc ttcaccggcc
      481 ttgcggtggc cgttgccttt cacgccggac tcttcaacat cggcggcgaa ggccaggcct
      541 acatggcggg cgccggtgct gccgccgttg gcttaagcct cgaatttttg ccctggccca
      601 tcgtgatccc gctttccatc ctcgcctcca tggcggtcgg cgccttctgg gcctgggtcc
      661 ccggcgtcct gcaggtgaag cgcggcagcc acattgtcat caccaccatc atgttcaact
      721 atatcgccct cagcctgatg gcctatctca tcaactacgg cttccgcccc gtgggcaaga
      781 tggcggtcga aagccgcgag atcaccggcg cctacatcat gagcttccgc gaagtggccc
      841 gcgagttctt tgacctgcgt ctgccctcga cgcccctgaa tttttccgtg ttcctcgcca
      901 tcgcggcggc gttcgccgtc tggtacctgc tttggcgcac caagcttggc tatgaaatcc
      961 gcgccgtcgg tgccagcccc gaggccgccg tctatgccgg catcaggccg gatcgcatca
     1021 tcgtcatcac catggcgatc tccggggcac ttgcggggct gatggccgta aacgagatcc
     1081 tgggcgccca gcaccggctc gttctcggct tcgtccaggg cgcgggcttt gtcggcatcg
     1141 cggtggccct catgggccgt tcccatcccc tggggataat gctttccgcc atgctctttg
     1201 gcctcctcta tcagggcggc actgaactct ccttcgccaa gcccgacatc aaccgcgaca
     1261 tgatcgtggt gattcagggc ctcgtcatcc ttttcatggg cgcgcttgaa tatgccttcc
     1321 gcccctggct caccgccctc ttcttcaggc cggaggactg accttcatgg agctctatgt
     1381 cgaaatcctc gcctccacca tccgttacac tgtgccgctc atcctcgcct gcatcgccgg
     1441 cctctggtcg gagcgttccg gcattgtcga catcggcctg gaaggcaaga tgctgatcgc
     1501 cgccttcgtc gccgcatcgg gtgctgcggt ttacggctcc gcctggatcg ggctggggct
     1561 tgccatactc acctcggtgg gctttgccct gattcatggc tatgccgcca tcaaccagcg
     1621 cggcaaccag gtggtctcag gcgttgccat caatatgctg gcggcgggcc tcgctgtggt
     1681 gctcggcaat gaatggttca gggaaggcgg ccgcaccccg gcactgccgc cggaaggccg
     1741 tttcctgccg ttggaaattc cggggatcag cgcccttgcc gatgtgcccg gcctgggcgc
     1801 tgttgtaaaa atatttttct cgcattctat tctcacttag aacagttttt cattaggtgg
     1861 tgttgctttg gc
//
LOCUS       DAORVN010000483         1872 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_68864_length_1872_cov_2.550358, whole genome shotgun sequence.
ACCESSION   DAORVN010000483 DAORVN010000000
VERSION     DAORVN010000483.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1872)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1872)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1872
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68864_length_1872_cov_2.550358"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="PKV23_09295"
     CDS             <1..227
                     /locus_tag="PKV23_09295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HQX85671.1"
                     /translation="ASKMPTLVKMIESLWLQAGPLLHLQQRVFAAQKHPVYVQHHRAI
                     QAIKSGKPAQVREAIVGDIVDAAEIISAAL"
     gene            complement(233..>590)
                     /locus_tag="PKV23_09300"
     CDS             complement(233..>590)
                     /locus_tag="PKV23_09300"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85672.1"
                     /translation="QMNALGDRLRAKLNDLASKHAVPMVATGFGSIFGIHFHEGKIRN
                     IDDLDQGEAGREAEVADLKKLFHLDMIDCGIYISRRIMGNLSVETTQAETDKLLAAVD
                     EFLASRKNLLKAVFNR"
     assembly_gap    591..600
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<601..1594)
                     /locus_tag="PKV23_09305"
     CDS             complement(<601..1594)
                     /locus_tag="PKV23_09305"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HQX85673.1"
                     /translation="MIHSALAPTLRNEQLSSVLRDAEARYVAANPRSAKLSGEAQGHL
                     PGGNTRTTVHFSPFPLYIAKGEGSRLTDVDGHSYIDFINEYTAGVFGHSNALIAETIS
                     RTIAQGLNFGAPTEHEVKLSAELRKRFPAMELLRFCNSGTEANLLAMATARAVSRKDT
                     IMIFNGAYHGSVFYFSHGPSPLNMPFPVITSTFNDPEKAASDIAKHGGKLAAVIVEPM
                     QGGAGALPATPAFIAALRDVTERHGVLLIFDEVMTSRVHFGGLQAYFGVKPDLVTLGK
                     YIAGGLTIGAFGGRADIMRRFDPSKPGSFPHGGTFNNNVVAMSAGYVALSRILTR"
     gene            complement(1667..>1872)
                     /locus_tag="PKV23_09310"
     CDS             complement(1667..>1872)
                     /locus_tag="PKV23_09310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HQX85674.1"
                     /translation="PETQAELVKHLTYGVPTLAAYDLMSQEIRDSLPTAPDKAAAAAV
                     YSDEYWVDQQAKATERFNTWVAQ"
BASE COUNT          400 a          520 c          535 g          407 t
ORIGIN      
        1 ccgcctcgaa gatgcccacg ctggtgaaga tgattgaatc gctgtggctt caggccggcc
       61 ccctgctgca cttgcagcag cgcgttttcg ccgcacagaa gcatccggtc tatgtgcagc
      121 accatcgcgc cattcaggcg atcaaatccg gaaaaccagc ccaggttcgg gaagcaatcg
      181 tgggcgacat cgtggatgcc gcggaaatca tcagtgcggc gctttagcgc tgtcatcggt
      241 taaacacggc ttttagcaga tttttccggc ttgccaggaa ctcgtccact gccgccaaca
      301 gcttgtcagt ttccgcctgg gtcgtttcga ctgaaaggtt tcccatgatg cggcgggaaa
      361 tatagatacc acagtcaatc atatcgaggt ggaacagctt cttgagatcg gccacttccg
      421 cctcgcgccc cgcttcaccc tgatcgagat catcgatatt gcgaatcttt ccctcgtgga
      481 aatgaatgcc gaagatcgac ccaaatccgg tcgcgaccat gggcaccgca tgtttcgatg
      541 caaggtcatt aagctttgcg cgcaggcggt cgcccagtgc gttcatctgg nnnnnnnnnn
      601 cgcgggtgag aattctgctc aatgccacgt atcctgcgga catggccacc acattgttgt
      661 tgaaggtgcc gccatgggga aatgatccgg gctttgacgg atcgaaccgc cgcatgatgt
      721 cggcccgccc gccaaacgcg ccaatggtga gcccccccgc aatatacttg ccaagtgtga
      781 ccaagtctgg ctttacgccg aaataagcct gcaacccacc gaaatgaacc cgagaagtca
      841 tgacttcatc gaaaatcaga agcaccccat gccgttcagt tacatcgcgt agcgctgcga
      901 taaaggcagg tgtggcaggc agcgcgccag ctccgccttg catgggctca acaatcaccg
      961 ctgcaagttt gccaccatgc tttgcaatgt cggaggccgc cttttccgga tcattgaagg
     1021 tggacgtgat aaccgggaat ggcatattga gcggcgaggg cccatggctg aaatagaaga
     1081 cactgccgtg ataggcaccg ttgaagatca tgatagtatc tttgcgggag accgccctag
     1141 cagtggccat ggcaagaagg tttgcctcgg tgccggaatt gcagaaccgc aaaagctcca
     1201 tagcggggaa acgctttcgc aattccgctg aaagtttcac ttcatgctca gttggcgcac
     1261 cgaagttcag gccttgcgcg attgtccgcg aaattgtctc ggcaatgagg gcgttggaat
     1321 ggccgaagac tccggcggta tactcattga tgaagtcgat ataactgtgg ccgtcaacgt
     1381 cggtgagccg gcttccctca cccttggcga tgtagagggg aaatggtgag aaatgcaccg
     1441 tggtgcgcgt atttccaccg ggcaaatgcc cttgggcttc tccgctgagt tttgcgctgc
     1501 gaggattggc tgccacgtaa cgtgcctccg catcgcgcaa aacggaggac agctgctcgt
     1561 ttcgcaaagt tggcgccaag gctgaatgta tcatggatgc tccatcgtaa ggcgggcggt
     1621 gcaaacggcg ggcagccatg aatgacgccc gccatattgc tgatttttac tgagcaaccc
     1681 aggtattgaa gcgttcagtc gcctttgcct gctggtcgac ccagtactcg tcactgtaga
     1741 cggctgctgc cgcggccttg tctggtgcgg tcggcagtga gtccctgatc tcctgggaca
     1801 tcaggtcata ggcggcgagc gtgggaacac cataagtgag gtgctttaca agctccgcct
     1861 gcgtttcggg ct
//
LOCUS       DAORVN010000484         1867 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_69194_length_1867_cov_2.386313, whole genome shotgun sequence.
ACCESSION   DAORVN010000484 DAORVN010000000
VERSION     DAORVN010000484.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1867)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1867)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1867
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69194_length_1867_cov_2.386313"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(136..1272)
                     /locus_tag="PKV23_09315"
     CDS             complement(136..1272)
                     /locus_tag="PKV23_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010970259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III PLP-dependent enzyme"
                     /protein_id="HQX85675.1"
                     /translation="MAATARIIDFLRDRRPEGPCLVVDLEVIADNYKAFTRAMPDTRI
                     FYAVKANPAPEVLKLLADLGSCFDTASVAEIEMALAAGATPERISYGNTIKKERDVAR
                     AHQLGISMFAVDCVEEVKKVSRAAPGARVFCRILTDGAGAEWPLSRKFGCDPDMATEV
                     LLHAHALGLKAYGISFHVGSQQTRLGAWDSALAEAKSIFDSLAGHGITLSMVNLGGGF
                     PTKYLKPVPGTATYANAIHDGLAKHFGNAIPETIIEPGRGMVGNAGVIKTEVVLISKK
                     HANDNLRWVYLDIGKFGGLAETMDEAIRYPIATARDDDEKTPCVIAGPTCDSADVLYE
                     KTPYDLPVTLTVGDEVLIGATGAYTTTYASVAFNGFSPLKAYII"
BASE COUNT          369 a          549 c          577 g          372 t
ORIGIN      
        1 ccgatgtctt gcggcggcgg gccttgccga aggcacgatt gagcagcgcc tcgcgtgcgg
       61 gcgcatccga ggcaagctcg tgacgaatgg tgaccatggc ggcctccaaa ggttacatgc
      121 gagaggaacg ggtgatcaga tgatgtaggc tttgagcggc gagaagccgt tgaacgccac
      181 cgacgcatag gtcgtggtat aggcgcccgt tgctccgatc agcacctcgt cacccacggt
      241 cagcgttacc gggagatcat aaggtgtctt ctcatagagc acgtcggccg aatcacacgt
      301 cgggcctgcg atcacgcagg gcgtcttttc atcatcgtcg cgcgcggtgg cgatcggata
      361 gcggatcgcc tcgtccatgg tttccgccag gccgccgaac ttgccgatgt cgagatagac
      421 ccagcgcagg ttgtcattgg catgcttctt cgaaatcagg accacttcgg tcttgatcac
      481 gccggcgttg cccaccatgc cgcggcccgg ctcgatgatg gtttcgggaa tggcattgcc
      541 gaaatgcttg gcaaggccat cgtgaatggc attggcatag gtagccgtgc ccggaaccgg
      601 cttgaggtat ttggtcggaa agccgccgcc gaggttgacc atggacagcg tgatgccgtg
      661 gcccgccagg ctgtcgaaaa tgctcttggc ttcggccagc gccgagtccc aggccccaag
      721 gcgggtctgc tgcgaaccca catggaagga aatgccgtag gccttgaggc caagcgcatg
      781 ggcgtgcagc agcacctcgg ttgccatgtc ggggtcgcag ccgaacttgc ggctcagcgg
      841 ccattcggct cccgcgccat cggtgaggat gcggcagaac acccgcgcac ccggagcggc
      901 gcgcgaaact tttttgactt cctcgacaca atccaccgcg aacatggaaa tgccaagctg
      961 atgcgcccgg gcaacgtcgc gctctttctt gatggtgttg ccgtaggaga tgcgctccgg
     1021 cgtggccccg gcggccagcg ccatttcaat ttcagcaact gaggctgtat cgaagcacga
     1081 accaagatca gccagaagct tcaggacttc cggggcaggg tttgccttca cggcataaaa
     1141 aatacgcgtg tccggcatgg ctcgcgtaaa ggctttatag ttgtcggcaa tgacctcgag
     1201 atcgacgacg aggcatgggc cttctggtcg tcggtcgcgg aggaagtcta tgatgcgggc
     1261 ggttgcggcc atgtgcgtat ctcccaaaaa agttgcgaca tcagcgctgt ctgaaaaccc
     1321 acgcgatgga gacgtggaat tgacaaaccg ctactgactt ttcgccttgg cttgatcggg
     1381 aaacccaatc gcactgcggg caatgatagt ggtgcctctc agtaacatca gcctttggag
     1441 ggccaatcga gaccaaaaag cccgcaccgt cgttgctttg gggatatcct tgaggcacgt
     1501 gcgactctgg gcatgcgcca tatggtggat tcgattgttg caatcaagaa aaaattacgt
     1561 ccggagcaaa gttttttctt aacagccgtt aatctttcgg cacgccgaaa accttccagc
     1621 ccgccagcag ccgcgtggcg actttggccc ggtacgccag tggaaccggt ttgccccctc
     1681 gtttcgcgcc cgccgccacg acccgctcag tcccccctca tcaatcgact ttcaggctga
     1741 gggcgccgtt gatgcgctcg ccggaattcc gaaggccccg gtatttctcg tcgaacaggt
     1801 tgctgccgtt tccggcgagg gcgacatcgc ggccgcgggg atcacgatca ccccggaggg
     1861 gaagagg
//
LOCUS       DAORVN010000485         1866 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_69274_length_1866_cov_2.215903, whole genome shotgun sequence.
ACCESSION   DAORVN010000485 DAORVN010000000
VERSION     DAORVN010000485.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1866
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69274_length_1866_cov_2.215903"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..460)
                     /gene="rplI"
                     /locus_tag="PKV23_09320"
     CDS             complement(<1..460)
                     /gene="rplI"
                     /locus_tag="PKV23_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018184225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HQX85676.1"
                     /translation="MEIILLERVAKLGSLGQVVKVKDGFARNFLLRRGKALRANEANK
                     AKFETQRAVLEAKNAERRKGAQDEAKGLDGKSFVIIRQAGESGQLYGSVSPRDIAEAA
                     STSGVAVERSHVLLDTPIKTIGMYTVTVAPHPEVEVKITINVARSADEAAA"
     gene            complement(473..715)
                     /gene="rpsR"
                     /locus_tag="PKV23_09325"
     CDS             complement(473..715)
                     /gene="rpsR"
                     /locus_tag="PKV23_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020178958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HQX85677.1"
                     /translation="MAMEQTARRPFFRRRKSCPFTGEGAPKIDYKDVRLLQRYISERG
                     KIVPSRISAVSAKKQRKLAQAIKRARFLGLLPYVLQ"
     gene            complement(715..1209)
                     /gene="rpsF"
                     /locus_tag="PKV23_09330"
     CDS             complement(715..1209)
                     /gene="rpsF"
                     /locus_tag="PKV23_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018390317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HQX85678.1"
                     /translation="MALYEHVFMARQDASPAQVEALVEQFKGVITAGGGKVGKVESWG
                     IRSISFRVKKNRKAHYTLMNIDAPYAAVAEMERLMLISEDVIRFMTVAVEEHEEGQSA
                     MLRRRDEREERGSFGDDRGFGFGGGGGGGGFGGGGGFGGRGGAGGGRGRPREDRVEPV
                     AEVL"
     gene            1486..1797
                     /locus_tag="PKV23_09335"
     CDS             1486..1797
                     /locus_tag="PKV23_09335"
                     /inference="COORDINATES: protein motif:HMM:NF020397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HQX85679.1"
                     /translation="MAGKAKFTTIDGYIAQFPKEVQAILEKIRQTVADAAPGAVEAIS
                     YQIPTFKLNGSNLVHFAAWKDHIGFYATPAGKDAKKMVDYILSVNCASVQKRARVGAR
                     I"
BASE COUNT          368 a          556 c          565 g          377 t
ORIGIN      
        1 gggctgcggc ttcatcggcc gagcgggcaa cgttgatggt gatcttcact tccacttccg
       61 gatgcggagc taccgttacc gtgtacatgc cgatggtctt gatcggcgtg tcgagcaaga
      121 cgtggctgcg ttcgacggcg acaccggagg tcgatgcggc ttcggcgatg tcacgcgggc
      181 taacggagcc atagagctgg cccgattcac cggcctggcg gatgatgaca aaggacttgc
      241 catcaaggcc cttggcctca tcctgggcgc ccttgcggcg ctcggcattt tttgcttcca
      301 gcacggcgcg ctgggtttcg aactttgcct tgttggcctc gttggcgcgc agcgccttgc
      361 cacggcgcag caggaagtta cgggcaaacc cgtcctttac ttttacgacc tggcccaagc
      421 tgccgagctt ggcgacgcgc tcaagaagaa tgatttccat gtttcaattc tcctattgca
      481 agacgtaggg aagcaggccc aggaagcggg cgcgcttgat ggcctgggcg agcttgcgct
      541 gcttcttggc ggaaaccgcc gagatgcggc ttggcacgat cttgccacgc tcggaaatgt
      601 aacgctgcag caggcgcaca tccttgtaat cgatcttggg cgcgccttca ccggtgaagg
      661 ggcagctttt ccggcgccgg aaaaacgggc ggcgggcagt ttgttccata gccattagag
      721 gacctccgcc actggttcga cacgatcttc acggggacgg ccacggccgc cgccggcacc
      781 gccgcggcca ccaaagccac cgccgccacc aaagccaccg ccgcctccgc caccaccgaa
      841 gccgaagccg cggtcatcgc cgaagctgcc gcgctcttcg cgctcatcgc gccggcgcag
      901 catggccgac tgaccttctt catgctcttc aacggcaacc gtcataaagc ggatgacgtc
      961 ttcgctgatc agcatgaggc gttccatttc ggccacagcc gcatagggcg catcgatgtt
     1021 catcagggtg tagtgggcct tgcggttttt cttcacgcgg aaggagatgg agcggatgcc
     1081 ccaggattca acctttccga ccttgccgcc gcctgccgtg atgacgccct tgaactgctc
     1141 gaccaaggct tcaacctggg ccggcgaggc atcctgacgg gccatgaata cgtgctcata
     1201 cagagccatg ttctgataac ctttctgttt ctcgtttcgt tatccgccat ccagcgcaga
     1261 gcctctgacg aaccagggaa aggttcgaaa gaccggtcaa acgtaagagc ggagacacgg
     1321 gaagacggga tttttcaccc tatctggcaa gccagacctt ccgttcagcc cccggctggg
     1381 tggtcgaagt aggggggtgt atggcggaaa aggctgatga aagcaagggc ggggatgcct
     1441 gctggccaag gttttaacca cgtccatttt gggctagtat agaccatggc cggcaaagca
     1501 aaattcacca caatagatgg ttatatcgcc caatttccaa aggaagttca ggccattctt
     1561 gaaaagatca ggcagacggt tgcggacgcg gctcccgggg cggttgaggc catcagctat
     1621 cagatcccga ccttcaagtt gaacggcagc aaccttgtgc atttcgccgc gtggaaggac
     1681 catatcggat tctatgcgac gcccgcgggc aaggacgcca agaagatggt cgactacatc
     1741 ctctcggtga attgcgcgtc ggtacaaaaa cgggctcggg tcggcgcgcg catctgatgg
     1801 atccctggaa gcgcgcctat gacgtgctcg gccatgtcgg cgggttgata ctcgccgtca
     1861 tggccg
//
LOCUS       DAORVN010000486         1865 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_69301_length_1865_cov_3.350829, whole genome shotgun sequence.
ACCESSION   DAORVN010000486 DAORVN010000000
VERSION     DAORVN010000486.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1865)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1865)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1865
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69301_length_1865_cov_3.350829"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1625
                     /gene="cysC"
                     /locus_tag="PKV23_09340"
     CDS             <1..1625
                     /gene="cysC"
                     /locus_tag="PKV23_09340"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018687305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HQX85680.1"
                     /translation="YRAFTTNKRKFIVADTPGHEQYTRNMATGASTADLAVILIDARK
                     GVLTQTRRHTFIATLLGIRHLVVAINKMDLMNYDQRVFEDIEADYRAFAKSIGADNIT
                     CIPLSALAGDNVVEPSAAMPWYHGPTLMAHLETVPVDDDLATKAFRLPVQWVNRPDQN
                     FRGFSGTIASGAVRPGDQIKVLPSAKTATVERVVTYNGDLPVGVAGQSLTITLTTEID
                     VSRGDVICNAADPIEVSDQFEVEVLWMGDEPMLPGRPYVIKTGNRQIPGTLDSLKYKV
                     NVNTMEHVAAKTLTLNEIGVCNLELDSSIAFTTYAENRHLGSFIIIDRFTNSTVGMGL
                     IHFALRRASNIHWQAMDVNKVSRAGQAKQKPTVLWFTGLSGSGKSTIANLVEKKLHAL
                     GHMTYLLDGDNVRHGLNRDLGFTDADRVENIRRVAEVSKLMVDAGLIVLVSFISPFRA
                     ERLMARESVEKDEFVEIFVDTALAEAEKRDVKGLYAKARRGEIKNFTGISSAYETPEN
                     PEISIDTAKLSAEQAAEMIVDYLDRQKRLTRA"
     gene            1683..>1865
                     /locus_tag="PKV23_09345"
     CDS             1683..>1865
                     /locus_tag="PKV23_09345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85681.1"
                     /translation="MRMLAIITLLAMAQPAIAMNWEGHEDDWRNEFPPAKAFLDAVPE
                     AKPLPSRDCPMGPLARA"
BASE COUNT          407 a          612 c          488 g          358 t
ORIGIN      
        1 cctatagggc cttcaccacc aacaagcgca aattcatcgt ggccgataca cctggccatg
       61 aacaatacac ccgcaacatg gcaaccggcg cctcgacagc cgatctggcc gtcattctca
      121 ttgacgcgcg aaagggagtg ctcacccaga cccgccgcca caccttcatc gccacgcttc
      181 tcggaatccg ccatctcgtc gtcgccatca acaagatgga cctgatgaac tacgaccaga
      241 gagtctttga ggatattgaa gccgattacc gcgcctttgc caaaagcatc ggcgccgaca
      301 atatcacctg catcccgctc tccgccctgg ccggggacaa tgtggtggag ccttccgccg
      361 ccatgccctg gtatcacggc ccaacgctca tggcgcatct cgaaaccgtg ccggtagatg
      421 atgacctagc caccaaggcc ttccgcctgc ccgtgcaatg ggtgaaccgc ccggaccaga
      481 atttccgcgg cttttccggc accattgcca gcggcgcggt gcgccccggc gaccagatca
      541 aggttctgcc gtctgccaag accgcaaccg ttgaacgcgt cgtcacctat aatggtgact
      601 tgccggttgg cgtggcgggc cagtcgctga ccatcaccct caccactgaa atcgacgtca
      661 gccgcggcga tgtgatctgc aacgcggcgg accccattga ggtgtcggat cagtttgagg
      721 tcgaggtatt gtggatgggt gacgagccca tgctgccggg ccgcccctat gtgatcaaaa
      781 ccggcaaccg ccagattccc ggcacgctcg attcgctcaa atacaaggtg aatgtcaaca
      841 ccatggagca tgtggcggcc aagaccctca ccctcaatga aatcggcgtg tgcaatctgg
      901 aactcgacag ctccatagcc ttcaccacct atgccgaaaa ccgccacctc ggcagcttca
      961 tcatcattga ccgctttacc aattcgaccg tcggcatggg cctcatccac ttcgccctgc
     1021 gccgcgcttc caacattcac tggcaggcca tggacgtgaa taaggtctcc cgcgccggcc
     1081 aggccaagca gaagccaacc gtgctgtggt tcaccggcct gtccggctcc ggcaaatcca
     1141 ccatcgccaa tctggtggaa aagaaactcc atgcgctcgg ccacatgact tacctgctcg
     1201 acggcgacaa cgtgcgccat ggcctcaacc gtgacctggg cttcaccgat gcggatcgcg
     1261 tggaaaacat ccgccgcgtc gctgaagtct ccaagctcat ggtcgatgcc ggattgatcg
     1321 tgcttgtttc cttcatctcc cccttccgcg ccgagcgttt gatggcaagg gaaagcgtgg
     1381 agaaggatga atttgttgaa atcttcgtgg acacggccct cgccgaagcc gagaagcgcg
     1441 acgtgaaagg cctctatgcc aaggcccggc gcggcgagat caagaacttc accggcattt
     1501 catcggccta tgaaaccccg gaaaatcccg aaatttcaat tgatacagcg aaactctcag
     1561 ccgaacaggc agccgaaatg atcgtcgatt atctcgacag gcagaaacgc ctgaccaggg
     1621 cttgaggacc agccctgtct ttagaacttt gaattgatct cacgcaggtt catactcggc
     1681 ccatgaggat gcttgccatt atcaccctgc ttgccatggc gcagccggcc atcgccatga
     1741 actgggaagg ccatgaagat gattggcgca atgaatttcc gccagccaag gcgtttctgg
     1801 atgctgtgcc cgaggcaaag ccgctgcctt cccgcgattg ccccatgggc cccttggccc
     1861 gcgcc
//
LOCUS       DAORVN010000487         1855 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_69966_length_1855_cov_3.060000, whole genome shotgun sequence.
ACCESSION   DAORVN010000487 DAORVN010000000
VERSION     DAORVN010000487.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1855)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1855)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1855
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69966_length_1855_cov_3.060000"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            61..951
                     /gene="htpX"
                     /locus_tag="PKV23_09350"
     CDS             61..951
                     /gene="htpX"
                     /locus_tag="PKV23_09350"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008873631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HQX85682.1"
                     /translation="MNTFRTGLLLAGLTGLFLAIGYLLGGQGGMMLAFLFAAGTNLFA
                     YWNSDKMVLRMQGAREVDRATAPEYYDIVATLAQRANLPMPKVYVMDNPQPNAFATGR
                     NPENAAVAATTGLLQTLSHSEVAGVMAHELAHIKNRDTLVMTLTATLAGAVSMLANFG
                     LLFGGRDRNSPLGPLGGLVLVILAPLAAMIVQLAVSRTREYEADRGGAEISGQPLSLA
                     SALHKISGAAHRIPNEAAEANPAMAHLFIVNPLSGQRMDNLFSSHPATENRVAELQKM
                     AAEMGTKETFQNPAPRGPWG"
     gene            955..>1855
                     /locus_tag="PKV23_09355"
     CDS             955..>1855
                     /locus_tag="PKV23_09355"
                     /inference="COORDINATES: protein motif:HMM:NF013216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HQX85683.1"
                     /translation="MSAAKPEKPGLMARRAAISLLQGITIDQLPLDQLLDTNAEFRLL
                     EGRDRSFAHALVASSLRHGGEITAVLGRFLAKPLPRSSGMAVDILTIAAAQLLFMEVS
                     PHAAIDLSVDLAKQDKKAQHFSGLINAVLRKVATDGKTALKNLDGPRLNTPDWLWEQW
                     VSAYGEETAHDIAASHLNEAPLDISVKDKPEYWAQQLKGELLPTGTIRLESGDGNLQE
                     VPGFSEGEWWVQDAAAALPAKLLGNIAGKSVLDLCAAPGGKTAQFASAGAVVTAVDDS
                     VTRMNRLRENLARLKLPVTTLLAD"
BASE COUNT          392 a          594 c          502 g          367 t
ORIGIN      
        1 tcttgtcgaa actcctgagc agcaccattt tatgctaaaa caaaggaaaa gtctcgaccc
       61 atgaacacct ttcgaaccgg acttctgctg gcaggcctca ccggcctctt cctcgccatt
      121 ggctacctgc tgggtggcca gggcggcatg atgctggctt ttctcttcgc cgccgggaca
      181 aacctgtttg cctattggaa ttccgacaag atggttttgc gcatgcaggg cgcccgcgaa
      241 gtcgatagag ccacagctcc cgaatactat gacatcgtgg ctaccctcgc ccagcgggcc
      301 aatcttccca tgccaaaagt ctatgtcatg gacaacccgc agcccaacgc ctttgccacg
      361 ggccgcaatc cggaaaacgc agcggtcgca gctacaaccg gacttctgca aacactctct
      421 cattctgaag tcgccggcgt catggcccat gagctggccc acatcaagaa ccgcgacacg
      481 cttgtcatga ccctcaccgc aaccctggct ggcgccgtat ccatgctggc gaattttggt
      541 cttctctttg gcggccgtga ccgcaacagc cccctgggtc ccctgggcgg ccttgtgctg
      601 gtcatcctgg cgccgcttgc cgccatgatc gtgcaacttg ccgtgagccg cacccgtgaa
      661 tatgaggccg accgcggcgg cgccgaaatt tccggacagc cgttgtcgct ggcctcagcc
      721 ctgcacaaga tttcaggagc cgcccatcgc attcccaatg aagccgccga ggccaatccc
      781 gccatggccc atctcttcat cgtcaatcct ttgtctggac agcgcatgga caatctgttc
      841 tcatcccatc ccgcaaccga aaatcgcgtt gccgagcttc agaaaatggc agcagagatg
      901 ggcaccaagg aaaccttcca gaacccagcg ccaaggggcc cttggggctg atcagtgtca
      961 gccgcaaaac ctgaaaagcc cggccttatg gccaggcgtg ccgctataag cctgctgcaa
     1021 gggatcacca tcgatcaact gccgcttgat cagctgcttg atacaaatgc cgaatttcgc
     1081 ctgcttgagg gccgcgaccg ctcctttgcc catgccctcg ttgcttcttc cctgcgccat
     1141 ggtggcgaaa tcacagcggt tctgggcagg tttctggcaa aacccctgcc gcgctcgtcg
     1201 ggcatggcgg tggatatcct gaccattgcc gcagcccagc tcctgttcat ggaggtgagc
     1261 ccccacgcgg ccattgacct ctccgtcgat ctggcaaaac aggacaagaa ggcccagcat
     1321 ttttccggct tgatcaatgc ggtgctgcgc aaggtagcaa ccgatggaaa aacggcgctg
     1381 aaaaatctcg acggtccaag actcaacacg ccggattggc tgtgggagca atgggtctcc
     1441 gcctatggcg aagaaacggc ccatgatatc gcagcctccc atctcaacga ggccccgctc
     1501 gatatttcag tcaaagacaa acccgaatat tgggcccagc agttaaaggg cgaactcctg
     1561 ccgaccggaa ccatccgttt ggaatccggc gacggcaatt tgcaggaagt gccgggattc
     1621 agcgagggcg aatggtgggt gcaggacgcc gcagcggcct tgcccgcaaa acttctcgga
     1681 aacattgcgg gaaaatcggt cctcgatctc tgtgcggctc ccggcggaaa aaccgcgcag
     1741 ttcgcatccg ctggagcggt tgtgaccgcc gttgatgact ccgtaacgcg catgaaccgg
     1801 ctgcgggaaa atcttgcgcg actgaagttg ccggtgacga cactgcttgc cgacg
//
LOCUS       DAORVN010000488         1851 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_70280_length_1851_cov_2.365813, whole genome shotgun sequence.
ACCESSION   DAORVN010000488 DAORVN010000000
VERSION     DAORVN010000488.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1851
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70280_length_1851_cov_2.365813"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(60..857)
                     /locus_tag="PKV23_09360"
     CDS             complement(60..857)
                     /locus_tag="PKV23_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010914224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HQX85684.1"
                     /translation="MKVYISVDIEGVAGITHWDEAEKGHADWAEFREIMTREAIAAID
                     GARAAGATEIWVKDAHDSGRNLITPMLPADVTLIREWSGHPYSMIQELDESFAALVMI
                     GHHAGVGSEGNSLAHTLNTRTHMMTLNGTPVSEFRLCALAAASLGVPVAFISGDKALM
                     EEVEQTNPHIGRLAVKEGRGQSTISMTPLAAQNAIQAGVEKSLRGKLKDCLLPLPEKF
                     TLDLTYVSPAMAYEKSFYPGCKYLGSRTNRVEAATIFDVMRALRFIK"
     gene            877..>1851
                     /locus_tag="PKV23_09365"
     CDS             877..>1851
                     /locus_tag="PKV23_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018185071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HQX85685.1"
                     /translation="MKIDANSRSVDLDPRAAQFYENPYPTYHELRRKVPAFKWQQYGY
                     WCFAGHEDVNALLRDRRFGRQILHVATREELGLADIPPRLKPFYDFEQHSLLELEPPV
                     HTRLRGLVNRAFLSRQVERLRPKIESLSNRLIDGFEAKGEVDLLPAYATPIPIIVICE
                     LLGVPADKADQLLNWSHDMVAMYMARRDREVEDAAVAATVAFSDYMRGLVKERRKDLN
                     DDLLSALIRAETEGQKLSENELITTAILILNAGHEATVHAIGNGVKTLLEHGVKEPVS
                     ESFIEEILRFDAPLHLFTRYALEDLKYGGIKFRKGEEVGLLLGAANRDP"
BASE COUNT          370 a          511 c          569 g          401 t
ORIGIN      
        1 ggcgacaagg aaaatgaatt ccttatgtga agtatgttcg ttggtcatca atcccaattc
       61 tattttatga aacggagcgc ccgcatcaca tcgaaaatgg tggcggcttc aacgcggttg
      121 gtgcgactgc cgagatattt gcagcccggg taaaagcttt tttcgtaggc catggccggg
      181 ctcacatagg tgaggtccag ggtaaatttt tccgggagcg gcaacaggca atccttcagt
      241 tttccgcgca aggatttctc gactcccgcc tggatggcgt tttgcgcggc aagcggcgtc
      301 atggaaatgg tggattggcc ccgcccctcc ttcacggcca gcctgccgat atgcgggttg
      361 gtttgctcca cttcttccat cagggccttg tcgccgctga tgaatgcgac gggaacgccc
      421 aggcttgccg ccgccagggc gcaaaggcgg aactcggaaa cgggtgtgcc gttcaatgtc
      481 atcatgtggg tccgggtgtt cagcgtgtgg gcgaggctgt tgccttcgga gcccacgccg
      541 gcatggtggc cgatcatcac aagggcggca aagctttcat ccagttcctg gatcatgctg
      601 tagggatggc ctgaccattc gcggatcaga gtcacatccg ccggtaacat gggggtaatc
      661 aggttacgcc cgctgtcatg ggcgtccttc acccagattt cggtggcgcc tgcggcacgc
      721 gctccgtcta tggcggcgat ggcttcacgg gtcataatct cgcggaattc ggcccagtcg
      781 gcatggccct tttcggcctc gtcccaatgg gttattccgg ccacgccttc aatatcgaca
      841 ctgatataga ctttcatttc agctaaacta gttcccatga agatagatgc caacagccga
      901 agcgtagacc tagacccgcg cgcggcgcag ttctatgaaa atccctaccc cacctaccat
      961 gagctgcgcc ggaaggtgcc agcattcaaa tggcagcaat atggctactg gtgttttgcc
     1021 gggcatgaag acgtgaacgc gctgctgcgc gaccggcgct tcgggcggca gatccttcat
     1081 gtggcgacgc gcgaggaact gggcctggcc gacattccac cgcggctcaa acctttctat
     1141 gacttcgagc agcactccct gctggagctt gagccgccgg ttcacacaag gctgcgcggg
     1201 ctggtgaacc gggcgttcct gtcacgccag gtggaacggc tgcggcccaa aatcgagagt
     1261 ctttccaacc ggttgatcga cggctttgag gctaagggtg aagttgacct gctgcccgcc
     1321 tatgccacgc ccattccgat catcgtgatc tgtgagctgc tgggcgtgcc tgcggataag
     1381 gcagatcagc ttttgaactg gtcgcatgac atggtggcca tgtacatggc acggcgggac
     1441 cgggaggtgg aggacgccgc cgttgcggcc actgtggcct tcagcgatta catgcgcggg
     1501 ctggtgaagg agcggcgcaa ggatctgaat gatgacctgc tctcggccct gatcagggcg
     1561 gaaaccgagg ggcaaaagct ttcggaaaac gagctcatca ccacggccat tcttattctc
     1621 aatgcgggcc atgaggccac ggtgcatgcc atcggcaatg gggttaaaac cctgcttgag
     1681 catggggtga aggagccggt cagcgaaagc ttcatcgagg aaattctgcg ctttgatgcg
     1741 ccgctgcacc tgtttacgcg ctatgcgctg gaagatttga aatatggcgg aatcaaattc
     1801 aggaagggcg aggaagtggg tttgctactg ggggccgcca atcgcgaccc t
//
LOCUS       DAORVN010000489         1850 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_70327_length_1850_cov_3.114206, whole genome shotgun sequence.
ACCESSION   DAORVN010000489 DAORVN010000000
VERSION     DAORVN010000489.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1850)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1850)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1850
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70327_length_1850_cov_3.114206"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..409
                     /locus_tag="PKV23_09370"
     CDS             <1..409
                     /locus_tag="PKV23_09370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85686.1"
                     /translation="IAFASSAVILGGCAPHLHSLRQDDSPDLVSGEFVHEGAPHLTLA
                     TRKRRYVAEGFEIHRHTDLAELQRRYKGTDPKHWDRIFAGHDRSHEVYSAEPIPRAQD
                     GAELSCRLTWGAGKPPQGICQDQSGKEYQVRFD"
     gene            747..992
                     /locus_tag="PKV23_09375"
     CDS             747..992
                     /locus_tag="PKV23_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003097518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2933 domain-containing protein"
                     /protein_id="HQX85687.1"
                     /translation="MNPETSHHHPEPWYRSRGGVVIAMIAVVVLFFVIREHWEHLTGR
                     WVYLLLLLCPLMHFFGHGGHGGHGGHGKTGGNSGESS"
     gene            976..1371
                     /locus_tag="PKV23_09380"
     CDS             976..1371
                     /locus_tag="PKV23_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009462698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HQX85688.1"
                     /translation="MGKVRRRLRVAGLIAAFSLPSLSVQAQGWQVPKPSPGLMPNPAV
                     GKKLFGTHCASCHGVALQGSDKGPPLLHRIYEPSHHADIAFQLAVKNGVRAHHWQFGD
                     MAPLPQITPDDVAHITAYVRAEQKKAGIR"
     gene            1635..>1850
                     /locus_tag="PKV23_09385"
     CDS             1635..>1850
                     /locus_tag="PKV23_09385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85689.1"
                     /translation="MKTSILVAAIVGSTLSMPILAAQDKAAPAKPAMSMDMDKQMPKM
                     QEKMKAMQAQMDKIHKTTDPAERQKLMQ"
BASE COUNT          423 a          518 c          555 g          354 t
ORIGIN      
        1 tatcgcattt gccagttccg ccgttattct tggcggttgc gcgccacatt tgcactcgtt
       61 gcggcaagac gattcgcctg atttggtcag cggagaattc gtccatgagg gcgcgccaca
      121 cctgaccctg gcgacaagga agcggcgtta cgtggcggag gggttcgaga tccatcgtca
      181 taccgacctg gccgaacttc aacgtcgcta caagggtacc gatcccaagc attgggatcg
      241 aatctttgcc ggtcacgacc ggagccatga agtctattcg gcagagccca taccaagagc
      301 gcaggacggg gccgaattgt cgtgtcgcct gacatgggga gcgggcaagc cacctcaagg
      361 catctgtcag gaccagtcag gaaaggaata tcaagttcgt ttcgattgaa agtcgtcagg
      421 tgaggaagac cgagcaccaa accattgttc aactcaagga gaaagtgatg tcgaagaaga
      481 tgtggatggt tgcggcagcg ttgctcacgg tattgctggg aggctgcgtt accagcggta
      541 gctattccgg gagtggttcc ggcagcgggt caagcggtgg ccactcccac tgacctcgtc
      601 cggaaactac ggagtgctgt ccgccgttgg cggatggtat tccgcgcttg gcgcgaggaa
      661 aacgccaagg cacacgctac ggcgaaaccc acggcctgct gttccgcacc gcctcccggc
      721 ggtaatcaaa aacgagaaag caaatcatga atccagaaac cagccatcac catcccgagc
      781 cgtggtatcg atcccgaggg ggtgtcgtca ttgccatgat cgcagtcgtc gtgcttttct
      841 tcgtgattcg tgagcattgg gagcatttga ccggtagatg ggtctatcta ttgcttctgc
      901 tgtgcccgct gatgcatttc ttcggtcacg gcggacacgg cggacatggc ggacatggca
      961 agacaggagg gaacagtggg gaaagttcgt aggcgtttgc gcgtggcagg actgatcgcg
     1021 gccttcagcc tgcccagtct gtcggtacag gcccagggct ggcaggtgcc caagcccagt
     1081 cccggcctga tgcccaatcc ggcagtcggc aagaaactgt tcggaacgca ctgcgcttcg
     1141 tgccacggcg ttgcgctgca agggtcggac aagggaccgc cgctcctgca tcgtatctat
     1201 gagccgtcgc accacgccga catcgcgttt caactcgcgg tcaagaacgg cgtgcgcgcc
     1261 catcactggc agtttggtga catggcgccg ctgccccaga ttacgcccga tgacgtggcg
     1321 cacatcaccg cctatgtgcg cgccgaacag aaaaaagcag gtatccgcta aggcatagcc
     1381 gtgaacgaag cggactctta gcaccgacat gaacactggt accaatgccc tgcgccgggc
     1441 gcgacaacaa gccatggaac cgccttgtgc ggcagcgtac agaccgtgat tggacttgca
     1501 tctaaaagag acattctcgc aattggtcat ccggactccg tcattgggaa ccggggccaa
     1561 tagcgcaagc cttggcgatt ggcagtcgcc aggaatgttg gcatcacgtt attcaaccct
     1621 gaaggaagac ctctatgaaa acctcaatac ttgttgctgc aatcgttggt tccaccttgt
     1681 cgatgcctat cctggcggct caggacaaag ctgcaccggc caagccggcg atgagcatgg
     1741 acatggacaa gcaaatgccc aagatgcagg aaaaaatgaa ggcgatgcag gcgcaaatgg
     1801 acaagatcca caagacgacg gatccggcgg aacgccagaa gctgatgcag
//
LOCUS       DAORVN010000490         1846 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_70636_length_1846_cov_3.132887, whole genome shotgun sequence.
ACCESSION   DAORVN010000490 DAORVN010000000
VERSION     DAORVN010000490.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1846)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1846)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1846
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70636_length_1846_cov_3.132887"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1116)
                     /locus_tag="PKV23_09390"
     CDS             complement(<1..1116)
                     /locus_tag="PKV23_09390"
                     /inference="COORDINATES: protein motif:HMM:NF013871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HQX85690.1"
                     /translation="MTKLPSLARARWLKKPSLQKVFALVAAAGGEARVAGGAVRNALL
                     GEPIGDIDIATTLAPQDVMAAFKAAGISVHPTGIEHGTVTVVVDHTPYEITTLRHDVE
                     TDGRRAVVAFTDDWKADALRRDFTINALYCDASGKIHDHVGGYADILRKRIMFVGAPA
                     ARIKEDKLRILRFFRFLASYEKMSADRASLAACVRLRKGLLGLSAERIAREMFKLLAG
                     PKAVPVLKLMARHKVLQNVLGHSDAFRVIGRLPPDPLLRAFALAKKPESLRAAWRLSN
                     QQSRRIEALLDGTVLTPKLRENERRILLYALGPEAWRDSVHLAWAGSRAALNDRTWKR
                     LLTLPSRWTAPVFPVTGHDLIDLGFPSGPDLGRELKRL"
     gene            complement(1113..1733)
                     /locus_tag="PKV23_09395"
     CDS             complement(1113..1733)
                     /locus_tag="PKV23_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006018972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HQX85691.1"
                     /translation="MKFSEAEIRRRAVTGLLHEVPLKHERSDDDMNRAAPFIPAGVKA
                     KPAAVLVPIVLREPEVTVLLTQRTDHLASHGGQVAFPGGRSDADDADAIAAALREAEE
                     ETGLARDFVEPLGFLDSYLTGTAFQVVPVVALVRPGFTVTPQESEVAAVFEVPLRFLM
                     TPENHATHSREWQGKQRFFYAMPYEERYIWGATAGMIRNLYDRLYA"
BASE COUNT          359 a          635 c          531 g          321 t
ORIGIN      
        1 cagccgcttg agttcgcggc ccagatccgg ccctgaggga aagcccaggt caatgaggtc
       61 atggcccgtg accggaaaaa ccggcgcagt ccagcgtgac ggcagggtga gcaggcgctt
      121 ccatgtccgg tcattcagcg ctgcccgcga acccgcccag gccagatgca cgctgtcgcg
      181 ccacgcctcc ggccccaggg cataaagcag gatgcgccgc tcattctccc gcagcttggg
      241 cgtaagcacg gtgccgtcca aaagcgcttc gatgcgccgg ctctgctggt ttgaaagccg
      301 ccacgccgcg cgcaaacttt ccggcttttt ggccagcgcg aaagcccgca gcagcggatc
      361 aggcggcagg cgccctatca cccggaacgc atcggaatgc cccaggacat tctgcaaaac
      421 cttgtgtctt gccatcagct tgagaaccgg caccgccttc ggtcccgcca gcagcttgaa
      481 catttcccgc gcaatgcgct cggccgaaag tcccagcagg cccttcctca ggcgcacgca
      541 ggccgcaagg ctcgccctgt cagccgacat tttttcatag gaggcaagga agcggaagaa
      601 gcgcaggatg cgcagcttgt cttccttgat gcgggcggcg ggcgctccga caaacatgat
      661 tcttttccgc aggatgtccg cgtaaccccc gacatggtca tggatttttc cggacgcatc
      721 gcaatacagc gcattgatgg tgaagtcccg gcgcagcgca tccgccttcc agtcatcggt
      781 gaaggccacg acagcgcgcc tcccatcggt ctccacgtca tggcgcagcg tggtgatttc
      841 gtaaggcgtg tgatcaacca ctacagtcac cgtgccatgc tcaatgccgg tgggatgaac
      901 cgaaatgccg gccgccttga aagccgccat cacatcctgc ggcgccagcg tggttgcaat
      961 gtcgatgtcg ccaatgggct cgcccagcag cgcattgcgc accgcgcctc cggccacgcg
     1021 cgcctctccg ccagccgcgg ccaccagcgc aaaaaccttt tgcaggcttg gctttttcag
     1081 ccagcgggca cgggcgagtg agggcagctt ggtcatgcgt agagccgatc ataaagattg
     1141 cggatcatcc ccgccgttgc cccccagata tagcgctcct catagggcat ggcatagaag
     1201 aagcgctgct tgccctgcca ttcgcgcgaa tgggttgcat ggttctccgg cgtcatcagg
     1261 aagcgcaggg gcacttcgaa cacggcagcg acttcgcttt cctgcggcgt caccgtaaac
     1321 cccggccgca ccagcgccac caccggcacc acctgaaagg cagtgccggt aagatagctg
     1381 tcaagaaatc ccaagggctc cacaaagtcc cgtgccagtc cggtttcttc ctccgcctcg
     1441 cgcagcgccg cggcaatcgc atcggcgtca tcggcatcac tgcggccgcc gggaaaagcc
     1501 acctgcccgc catggctcgc gaggtggtcg gtgcgctgcg taagcagtac cgtcacctcc
     1561 ggctcgcgca gcacaatggg caccagcacg gcggcgggct ttgccttcac gccagccgga
     1621 atgaacggcg ccgcccggtt catgtcatca tcgctgcgtt catgcttcag cggaacttca
     1681 tgcaacaggc ccgtcaccgc acgccggcgt atctcagcct cgctaaattt catcggcgcg
     1741 ctgcattgga aagaattcac ctgacgacca caccccgaac cattcgcctt cgacggtgcc
     1801 cttggccacc agatcataga acaaggcgcg cgacactagc gcctca
//
LOCUS       DAORVN010000491         1844 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_70779_length_1844_cov_2.765791, whole genome shotgun sequence.
ACCESSION   DAORVN010000491 DAORVN010000000
VERSION     DAORVN010000491.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1844)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1844)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1844
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70779_length_1844_cov_2.765791"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..269
                     /locus_tag="PKV23_09400"
     CDS             <1..269
                     /locus_tag="PKV23_09400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HQX85692.1"
                     /translation="RSARGGRKPGRHREKPADRAPAAKMPAAAKPVAARPAVEQPIAE
                     KPAPQAKPPRQPEQRRARPERELEDAPESGGFHKGNMPAFLLRK"
     gene            complement(374..721)
                     /locus_tag="PKV23_09405"
     CDS             complement(374..721)
                     /locus_tag="PKV23_09405"
                     /inference="COORDINATES: protein motif:HMM:NF016854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HQX85693.1"
                     /translation="MSVSASFKEFLIEQMAGFGPVTIRPMFGGAGVYHDGLMVALVAD
                     EVLYLKVDEASRPAFIAENLPPFVYSRKDQKIDMSYFRSPERCMDDVDEMAIWCRTAY
                     GAALNAARKKPKK"
     gene            complement(718..1095)
                     /gene="sufA"
                     /locus_tag="PKV23_09410"
     CDS             complement(718..1095)
                     /gene="sufA"
                     /locus_tag="PKV23_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012044045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HQX85694.1"
                     /translation="MAQATPRPRPKVMTLTDAAAARVKAIIARSDTPVVGLRVGVKNG
                     GCAGMEYTMDWATEQKPFDEVIEEKGVKVLIDPKAILFLLGTEMDFQTSALKSGFTFN
                     NPNQTSACGCGESVQLKQAEATA"
     gene            complement(1105..1401)
                     /locus_tag="PKV23_09415"
     CDS             complement(1105..1401)
                     /locus_tag="PKV23_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010685953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system Fe-S cluster assembly protein"
                     /protein_id="HQX85695.1"
                     /translation="MTDDIVSALKTVYDPEIPVDIYELGLIYKVDIDDDRNVAIDMTL
                     TAPGCPVAGDMPGWVENAVSTVPGVASAKANLVFDPPWDHSRMSDEARVAMNMW"
     gene            complement(1464..>1844)
                     /locus_tag="PKV23_09420"
     CDS             complement(1464..>1844)
                     /locus_tag="PKV23_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008546968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HQX85696.1"
                     /translation="VQAIGLGAALDYMTSIGLENIAAHEADLRDYATQRLSEINSLRI
                     FGRAKDKGAILSFNMNGAHAHDVATVIDRAGVAVRAGTHCTQPLLQRFGVTATCRASF
                     AMYNTRDEVDKLAEALISAQRIFL"
BASE COUNT          365 a          518 c          571 g          390 t
ORIGIN      
        1 agcgttcggc gcgcggcggc agaaaacctg gccgtcaccg ggaaaagcct gcggacagag
       61 cgccggctgc caaaatgccc gctgctgcaa agccagttgc tgccagacca gctgttgaac
      121 agcccatcgc tgaaaagccc gcaccgcagg ccaagccgcc acgccagcca gagcagcgcc
      181 gggcaaggcc cgagcgggaa cttgaagacg cgccagaatc cggcggtttc cacaagggca
      241 acatgccggc gtttttgctg cggaaataga taactaagcc aacatattcc gtcaccccgg
      301 cgaaagccgg attctccttg aagtcagagg gattccggct tgcgccggaa tgacgagttt
      361 tttggatggt ggattatttt tttggctttt tccttgccgc gttcagcgcc gcgccgtagg
      421 cggtgcggca ccagattgcc atttcatcga cgtcgtccat gcagcgctcg gggctgcgaa
      481 aatacgacat gtcgatcttc tggtctttcc tggaatagac aaagggcggc aggttctccg
      541 ctataaatgc tggtctactg gcttcatcga ctttgagata gagcacttca tcagccacca
      601 gggcgaccat taggccatcg tgatagacgc cggcgccgcc gaacatgggg cggatagtga
      661 cgggaccaaa gcccgccatt tgctcgatga ggaattcctt gaagctggcg ctgacgctca
      721 agcggttgct tcagcctgtt tcaactggac gctttcgccg caaccgcagg ctgaggtctg
      781 gtttggattg ttgaaggtga agccggattt gagggccgac gtctgaaaat ccatttcggt
      841 gcctaacagg aacaggatgg cctttggatc gatcaggacc ttcacgccct tttcctcaat
      901 cacttcatcg aaaggcttct gctcggtggc ccaatccatg gtgtattcca taccggcgca
      961 gccgccgttt ttgacgccga cgcgcagtcc aacgacgggc gtgtccgacc tggcaatgat
     1021 cgcctttacc cgtgccgcgg cggcatcggt cagggtcatc accttggggc ggggtcttgg
     1081 agttgcctgg gccatgttcc tatcctacca catattcatc gcaacgcgcg cttcatcgga
     1141 catccggcta tgatcccacg ggggatcaaa caccaggttc gccttggcgc tggctacacc
     1201 gggcacggtc gaaacggcgt tttcaaccca gcccggcatg tcgcctgcca cggggcagcc
     1261 cggggcggtc agggtcatgt cgatggcgac gttgcggtca tcgtcgatat ccaccttata
     1321 gatgaggccc agctcataga tatcaacggg aatttccggg tcgtagacgg tcttgagggc
     1381 tgacacgata tcatcggtca tacgggccaa ttcatcggcc gggatggcgg aggggtttgg
     1441 ggcgttgtcg gtcgcaaggt ctgtcacagg aatatccttt gtgcagagat aagggcttcg
     1501 gccaatttat caacttcgtc gcgggtgttg tacatggcaa atgaggcccg gcaggtggca
     1561 gtgacgccga aacgctgcag cagtggctgg gtgcaatggg taccggcgcg gaccgccacc
     1621 cctgcccggt caatgacggt tgccacgtca tgggcgtggg ctccgttcat attgaaggag
     1681 aggatggcgc ccttgtcttt ggcgcggcca aaaatacgga gagaattgat ttccgagagg
     1741 cgctgggtgg catagtcccg gaggtccgct tcatgggcgg cgatgttttc gaggccgatg
     1801 cttgtcatgt aatcgagtgc cgcgccgagg ccgatggcct ggac
//
LOCUS       DAORVN010000492         1842 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_70865_length_1842_cov_4.364298, whole genome shotgun sequence.
ACCESSION   DAORVN010000492 DAORVN010000000
VERSION     DAORVN010000492.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1842
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70865_length_1842_cov_4.364298"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..657)
                     /gene="argB"
                     /locus_tag="PKV23_09425"
     CDS             complement(<1..657)
                     /gene="argB"
                     /locus_tag="PKV23_09425"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011995211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HQX85697.1"
                     /translation="MTDIPIPETARILSEALPFLQRYDDQIIVVKFGGHAMVDRKLSQ
                     QFARDMVMLKVCGLIPIVVHGGGPQINKMLDKLSVKAEFREGLRVTDQETMDVVEMVL
                     AGSINKSIVASIQQAGGRAVGISGKDGSLMIAERYVKQKTDAKTGKVENIDFGFVGEP
                     ARINIEILQTIMKSDAIPVIAPIGVGWDGDTYNVNADTAAGAIATATQAKRLLMLTDV
                     A"
     gene            complement(732..1364)
                     /gene="yihA"
                     /locus_tag="PKV23_09430"
     CDS             complement(732..1364)
                     /gene="yihA"
                     /locus_tag="PKV23_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014283915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HQX85698.1"
                     /translation="MTSRFTSDQLLAGRRLFSAPSTFMLGVAALHQLPPSTGVEIAFA
                     GRSNVGKSSLINALTGVRELARASNTPGRTRELNFFNIADALTLIDMPGYGYAKAPKK
                     EVKKWQSMLRGYLRGRPGLTRAFVLIDARHGVLAADEEMFGLLDEAAVTYQLVLTKID
                     KLKPAEVEKVYAETEAAAKKRAAAFPLIHATSSEKLAGLEDLRAEIAGLL"
     gene            complement(1361..>1842)
                     /gene="yidC"
                     /locus_tag="PKV23_09435"
     CDS             complement(1361..>1842)
                     /gene="yidC"
                     /locus_tag="PKV23_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007607736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HQX85699.1"
                     /translation="LSGCLPVVVQIPVFFSLYKVILTSIELRHAPFFGWVHDLSAADP
                     TNLFTLFGLIPWSPPLFLVLGIWPILMGVTMWVQMRLNPTPPDPVQASLFNWMPVIFT
                     FMLGSFPAGLVIYWAWSNFLSILQQSYIMKRHGVEIDLLGNIRDSLPFLKKKPAATT"
BASE COUNT          379 a          508 c          603 g          352 t
ORIGIN      
        1 ggccacatcg gtcaacatga gaaggcgctt ggcctgggtg gcggtggcaa tggcgccagc
       61 cgccgtgtcg gcgttcacgt tgtaggtatc gccatcccag cccacgccga tgggggcgat
      121 caccggaatg gcgtcactct tcatgatggt ctggagaatt tcaatgttga tgcgggccgg
      181 ttcgccgaca aagccaaaat cgatgttctc caccttgccg gttttcgcat cggttttctg
      241 cttcacatag cgctcggcga tcatcaggct gccgtccttg ccggaaatgc ccacggcgcg
      301 gccgccggcc tgctggatcg aggcgacgat ggatttgttg atggagcccg ccagcaccat
      361 ttccaccacg tccatggttt cctggtcagt gacgcgcagg ccttcgcgga actccgcctt
      421 caccgaaagc ttgtcgagca tcttgttgat ctgggggccg ccgccgtgga ccacaatggg
      481 gatcagcccg cagaccttca gcatcaccat gtcgcgggcg aactgctggg acagcttgcg
      541 gtcaaccatg gcatggccgc caaatttcac cacgatgatc tggtcgtcat agcgctgcag
      601 aaagggcaag gcttccgaga ggatgcgggc cgtttcggga attggaatgt cagtcatggc
      661 gggattccag cagttatggc atggcgttat agggcaattg ccgggcttgc caagattgaa
      721 gtttaccggg gttaaagaag gcctgcgatt tcggcgcgga ggtcttccag gccggcaagt
      781 ttctcgctcg aggtggcatg gatcagcgga aaggcggcgg cgcgcttttt cgcggcagcc
      841 tcagtctccg cataaacctt ttcaacttcg gcaggcttga gcttgtcgat cttggtaagg
      901 acgagctgat aggtgacggc cgcttcatcg agaaggccga acatttcctc gtcggcggcg
      961 agcaccccat ggcgcgcgtc aatcagcacg aaggcgcgtg tcaatccggg ccttccccgg
     1021 agatagccgc gcagcatgga ctgccatttc ttcacttcct tcttcggcgc cttggcatag
     1081 ccgtagccag gcatgtcgat aagggtgagg gcatcggcga tgttgaagaa attgagctcg
     1141 cgggtgcggc ccggcgtgtt ggaggcccgg gccagttccc tgaccccggt cagggcattg
     1201 ataagggaag acttgccgac attggaacgg ccggcaaagg cgatttcaac gccagtggac
     1261 ggcggcagct ggtgaagtgc cgccactccc agcatgaagg tggagggggc cgaaaacagc
     1321 cggcggcccg ccaggagctg gtcgctggtg aagcgcgagg tcaagtggtg gccgcgggct
     1381 tctttttcag gaagggcagg ctgtcgcgga tgttgccgag aaggtcaatc tcaacgccat
     1441 ggcgcttcat gatgtaggat tgctggagga tcgacaggaa gttgctccag gcccagtaga
     1501 tcaccagacc cgccgggaag cttcccagca tgaaggtgaa gatcaccggc atccagttga
     1561 acaggctggc ctgcaccgga tcgggcggag tggggttcag ccgcatctgc acccacatgg
     1621 tcacgcccat gagaatcggc cagatgccaa ggaccaggaa aagcggcggt gaccagggaa
     1681 taaggccgaa gagcgtgaac aggttggtgg ggtcggcggc ggacagatca tgcacccagc
     1741 caaagaacgg cgcgtggcgc aattcaatgg aagtgaggat caccttgtaa agcgagaaga
     1801 ataccggaat ctgcacaacg accggcaagc aacccgacag tg
//
LOCUS       DAORVN010000493         1842 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_70909_length_1842_cov_2.327364, whole genome shotgun sequence.
ACCESSION   DAORVN010000493 DAORVN010000000
VERSION     DAORVN010000493.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1842
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70909_length_1842_cov_2.327364"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="PKV23_09440"
     CDS             complement(<1..300)
                     /locus_tag="PKV23_09440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85700.1"
                     /translation="MTAIMPLQGFETNHSGWIAATGLRLVRARPTIVVGARAMLLLLP
                     TNTSATTTGPATRLAVRHGVAALTAATVGFIAGDTVTRSELNFELDDFVPLFVGSI"
     gene            299..1462
                     /locus_tag="PKV23_09445"
     CDS             299..1462
                     /locus_tag="PKV23_09445"
                     /inference="COORDINATES: protein motif:HMM:NF005788.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiH/UbiF family hydroxylase"
                     /protein_id="HQX85701.1"
                     /translation="MQQFDLIIVGGGLAGASLALALRETRLRIALVESHSPSRPEGWD
                     ARIYAISPANAAFLQSIGAWRHFESARMAPIRAMQIHGDGSGRLDFSAFETGVSKLGW
                     IVESSLMACEFWESAKRQANLTLFCPACPEGLEFRHDAAVLRLRDGTVLSSRLLVGAD
                     GRDSWVRQSAGLAAINTPYGEKGVVANFSTEKAHLNTAYQWFRDDGVLAYLPLPGNQI
                     SIVWSTPDDHADTLCELSPALLAERVAEAGSFTLGRLEPLTPPAAFPLRLMRVPQTVA
                     PRLALVGDAAHGIHPLSGHGINLGFQDARELACLLAATQPWQDIGEQRLLQRYQRARR
                     EETLLMQTATDGLRRLFKNSSPGLRPLRNFGLGLTNGLPILKNALVRYALGAL"
     gene            1472..>1842
                     /locus_tag="PKV23_09450"
     CDS             1472..>1842
                     /locus_tag="PKV23_09450"
                     /inference="COORDINATES: protein motif:HMM:NF021896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide isomerase DsbC N-terminal
                     domain-containing protein"
                     /protein_id="HQX85702.1"
                     /translation="MLKQLFPMLLAASCTSMAVADEAEIKKAMEAKLGTKVESVTKSG
                     YLGLFEVYADGQLLYTDEKLTAIVVGAQLIDAKTMKNVTDERMKKLTAIKFSELPLER
                     AIKQVRGDGKRVMATFEDPNCG"
BASE COUNT          345 a          525 c          580 g          392 t
ORIGIN      
        1 gatcgaaccg acgaacaatg ggacgaaatc atcgagctcg aaattcagct cgctccgggt
       61 aaccgtatct ccggcaatga atcccacggt ggcggcagtc aaggccgcaa ccccatgtcg
      121 cacggcaagc ctggtggcgg ggccggtggt ggtggcggag gtgttggtgg gcaacagaag
      181 aagcatcgcc cgcgcaccaa caacaatcgt cggccgcgcc cgaacaagcc gcagtccggt
      241 ggcggcaatc cagcctgagt ggtttgtttc aaagccctga agcggcataa ttgccgtcat
      301 gcagcagttt gacctgatca ttgtcggcgg tggcctggcc ggcgccagtc tcgctttagc
      361 tttgcgcgag acgcgcctgc gcatcgcgct ggtggagagc cactcgccga gcaggcctga
      421 aggttgggat gcacgtatct atgcgatcag tccggccaat gctgcattcc tgcagtccat
      481 aggcgcgtgg cggcatttcg aaagcgcccg catggcgccg attcgtgcca tgcaaattca
      541 tggtgatggc agcggtcggc tcgatttctc cgcctttgag acaggtgttt ccaaactggg
      601 ctggattgtc gagtcctccc tgatggcttg cgagttctgg gaaagcgcca agcggcaggc
      661 caatctgacg cttttctgtc cggcatgccc ggaggggctg gaattccgcc atgacgctgc
      721 agtgctccgc ttgcgcgacg gtaccgtgct ctcctcgcgc ctgctggtcg gtgccgatgg
      781 ccgggattct tgggttcgtc agtcggccgg gttggccgcc atcaatacgc cgtacggcga
      841 gaagggcgtg gtcgccaact tttcgactga aaaggcgcat ctcaatacgg cctaccagtg
      901 gttccgagac gatggtgttc tggcttacct gccgcttccc ggcaaccaaa tctccattgt
      961 gtggtccacc ccggatgatc atgccgatac cctttgtgaa ctgtcgccgg cattgttggc
     1021 cgagcgtgtg gccgaggctg gttcattcac cctcggacgg cttgagccac tgacgccccc
     1081 ggcggcgttt cccttgcgat tgatgcgcgt gccgcagacg gttgcgcccc gcttggcact
     1141 ggttggagat gcggcgcatg gcattcatcc cttgtccgga cacggtatca accttggttt
     1201 tcaggacgca cgcgaactgg cctgtctgct ggccgcaacg caaccctggc aagatatcgg
     1261 cgagcagcgt ttgttgcagc gctaccagcg cgctcgccgc gaagaaacgt tgttgatgca
     1321 gaccgccact gatggcttgc gccggctgtt caagaactcg tcgccgggat tgcgcccatt
     1381 gcgcaatttt ggcctgggcc tgacaaatgg cctgcccatt ttaaagaatg ccctggtgcg
     1441 ctatgcgctt ggcgcactat aggagaatcg catgttgaag cagttgtttc cgatgctact
     1501 ggccgcttcc tgcacgtcca tggctgttgc cgacgaggcc gaaatcaaga aggccatgga
     1561 ggccaagctc ggcaccaagg ttgagagcgt aaccaagtcg ggctacctgg ggcttttcga
     1621 ggtctatgcc gatggtcagc ttttgtatac ggatgaaaag ctgactgcga ttgtggtggg
     1681 ggcacagttg atcgatgcca agaccatgaa gaacgttact gacgagcgca tgaagaagct
     1741 gactgcgatc aagttcagtg aattgcctct ggagcgtgcc atcaagcagg tgcgcggcga
     1801 tggcaagcgg gtcatggcaa cgttcgagga tccgaactgc gg
//
LOCUS       DAORVN010000494         1840 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_71012_length_1840_cov_3.435294, whole genome shotgun sequence.
ACCESSION   DAORVN010000494 DAORVN010000000
VERSION     DAORVN010000494.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1840
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71012_length_1840_cov_3.435294"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..499
                     /locus_tag="PKV23_09455"
     CDS             <1..499
                     /locus_tag="PKV23_09455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85703.1"
                     /translation="PPEESPVEPEVEAEAPVPSSPMPSPMSPLSYYQLLSRMTPAELG
                     RERMVLAALPRSPNTQLRLAMLFGYPRAQQDIARAMTQLDLVLKSNDPAAVALYPLAR
                     LLAHNYAERQKMDVHLERQGQQLKESQRKAVELQEKIDGLADIERTLPQPPRAVRPAR
                     SGGAK"
     gene            496..>1840
                     /locus_tag="PKV23_09460"
     CDS             496..>1840
                     /locus_tag="PKV23_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013358747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HQX85704.1"
                     /translation="MTGVAGGSGAHILVIDDDHDILRLLSMRLRARGFRVSAAGSAEE
                     GLARITVDPPRLVVSDIRLPGRDGLSLFEELRSTRPSLPVILLTAHGTIPDAVDATSK
                     GVFGYLTKPFDSQLLLEKIDQALQLSAPSLPADSVTPGGGANPNEKWLAGVIFRSSRM
                     AHLMAEARMVAASDASVLIRGESGSGKEVLARAIHRASARAKGPFVAINCGAIPEPLL
                     ESELFGHAKGAFTGAANSRVGLIQAAHGGTLFLDEIGDMPPALQVKLLRVLQERVVQP
                     VGATKSEPVDVRLLSATHRDLDAAMAQGQFREDLYYRLDVVSLTLPRLDERREDVPLL
                     AAHFVSLLAGKYKKPISGFAPDALEALATASWPGNVRQLYNVVEQSCALTSTPLIPLS
                     LVQRALRVPSLEALTYADAKQRFERNYLVQLLKLTDGNVADAARIADRNRTEFYRL"
BASE COUNT          346 a          558 c          553 g          383 t
ORIGIN      
        1 ccctccggag gaaagccccg tcgagccgga agtagaggca gaggcgcccg tgccctcgtc
       61 accaatgcct tcaccaatgt ctcccctgag ctattaccag ctactttccc ggatgacgcc
      121 cgccgaactg gggcgtgaac ggatggtgct ggccgcgttg ccacgttcac caaataccca
      181 gttgcggctt gccatgctgt tcggctatcc gcgtgcccaa caggatatcg cccgggcgat
      241 gactcaactc gatctcgtgt tgaaatcgaa cgatcctgct gcggttgcct tgtatccgtt
      301 ggcgcgattg ctggcccata actatgctga acgccagaaa atggatgtgc atctggaacg
      361 gcaggggcag caattgaagg aaagccagcg gaaggccgtc gagctgcagg aaaaaatcga
      421 tggcctggcc gatatcgagc ggaccttgcc acaacctccc cgagccgttc gtcctgccag
      481 gtcggggggg gcgaaatgac aggcgtggca ggagggagtg gagcgcacat tttagtcatt
      541 gatgatgacc acgatatttt gcggcttctc tcgatgcgcc tgcgggcacg aggttttcgg
      601 gtcagtgccg ccggctcggc cgaagagggt ttggcgcgaa taaccgttga cccgccacga
      661 ctcgtcgtca gcgacatccg tctgcccggc cgggatggtc tgtccctgtt tgaggagtta
      721 cgcagcacac ggccaagctt gccggtaatc ctgctgaccg ctcacgggac catcccggat
      781 gcggtcgacg ctacttccaa aggggttttc gggtatctga caaaaccgtt tgacagccag
      841 cttctgctcg aaaaaatcga tcaggctttg cagctttcgg cccccagcct gccggcggat
      901 tcggtgactc cggggggcgg ggccaatccg aatgaaaagt ggctggccgg cgtcattttc
      961 cgcagcagcc gcatggccca tttgatggcg gaggcccgta tggttgctgc ttccgatgcc
     1021 agcgtgctga ttcggggcga aagcggttcg ggcaaggagg ttctggcacg ggctattcac
     1081 cgggccagcg cccgtgccaa ggggccgttt gtcgccatca actgcggcgc cattcccgag
     1141 cccctgcttg aatccgagtt gtttggccac gccaaaggtg cctttaccgg cgccgccaac
     1201 agccgcgtcg ggcttattca ggccgcgcat ggtggcaccc tctttctcga cgaaatcggt
     1261 gatatgccgc ccgcgctgca ggtcaaattg ctgcgtgtac tgcaagagcg cgttgttcag
     1321 cccgttggtg cgaccaaatc tgaaccggtt gacgttcgct tgctctcggc aacccatcgc
     1381 gatctcgatg ctgcgatggc gcaaggccag tttcgtgaag acctttatta ccggctcgat
     1441 gtcgtctcac tgaccctgcc gcgtcttgat gagcgtcgcg aagatgttcc cctgctggcg
     1501 gcacatttcg tcagcctcct tgccggcaag tataaaaagc ccatcagcgg ttttgcgccg
     1561 gacgcgctgg aagcgctggc gacggcatcc tggccaggca atgtacggca actttacaat
     1621 gtggttgaac aaagctgtgc actgacctca acgcccctga taccgctttc actcgtccag
     1681 cgggccttgc gcgtaccatc ccttgaggca ttgacctatg ccgatgccaa gcagcgtttc
     1741 gagcgcaatt acctggtcca gctactcaag ctgaccgatg gcaacgtggc ggatgcggcg
     1801 cggatcgccg atcgcaaccg aaccgagttc tatcgcctga
//
LOCUS       DAORVN010000495         1838 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_71178_length_1838_cov_2.367358, whole genome shotgun sequence.
ACCESSION   DAORVN010000495 DAORVN010000000
VERSION     DAORVN010000495.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1838
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71178_length_1838_cov_2.367358"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            17..1033
                     /gene="bmt"
                     /locus_tag="PKV23_09465"
     CDS             17..1033
                     /gene="bmt"
                     /locus_tag="PKV23_09465"
                     /EC_number="2.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007597759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine--homocysteine S-methyltransferase"
                     /protein_id="HQX85705.1"
                     /translation="MTRPTFTEALNSRPWLLADGATGTNYFQMGLVSGDAPEMWNFEH
                     PERVRKLHREFIQAGADIVLTNTFGGNRHRLKLHNEQGRVREINMAAAALARAEADAA
                     GRTVYVGGSIGPTGEIFMPVGTMPHEEGVSAFEEQAMALKEGGVDVLWIETMSSEEEL
                     RAAVEGAAKAGLPLVTTMSFDTNGRTMMGITPKAFGALTASLGTQPVAIGANCGVGAS
                     ELVATVLGISEARPDAAIVAKGNCGIPQYKEGHIHYTGTPELMADYARIALDSGAKII
                     GGCCGTSPEHLAAMRKSLESYTKGPRPSIELVEQRLGPVSVLAKGIDTAAEGAAKRER
                     RRGN"
     gene            1106..>1838
                     /locus_tag="PKV23_09470"
     CDS             1106..>1838
                     /locus_tag="PKV23_09470"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HQX85706.1"
                     /translation="MDQAVFLRGETWGFGGQPVEHIETHAAHVFLVGDRAFKMKKAVK
                     LPYLDFSTIEKRRAVLAHELEINRPFAPSVYLRTDEIRGEPVLVMKRFDQQALLSSQA
                     GGITDATAKELARMVAESHVIAPQRDTPGSEIMAGLGLQLSKAFADSPDIFPVDEAGK
                     FELLHEAHLTRLRPLLDQRSAQGLVRRCHGDMHCGNIVLLEGKPNLFDAIEFSEKIAT
                     IDVLYDLAFLLMDLGRRGEKRVANLV"
BASE COUNT          357 a          569 c          555 g          357 t
ORIGIN      
        1 atttcctgag gctcccatga cccgaccgac cttcaccgaa gcgcttaatt cccgtccctg
       61 gcttctggct gacggcgcca ccggcaccaa ctatttccag atgggcctgg tgtcgggcga
      121 tgcgccggag atgtggaatt tcgagcatcc ggagcgggtg cgtaagctcc accgcgaatt
      181 catccaggct ggcgccgata tcgttctcac caacactttt ggcggaaacc gccaccgcct
      241 gaaactgcac aatgagcagg gccgggtgcg cgaaatcaac atggccgccg cggcactcgc
      301 ccgcgctgaa gcggatgcgg ccggccgcac cgtttatgtc ggcggctcca ttggccccac
      361 cggagaaatc ttcatgcccg tcggcaccat gccccacgag gagggtgttt ccgccttcga
      421 ggagcaggcc atggccctga aggagggcgg cgttgacgtg ctgtggattg aaaccatgtc
      481 gtccgaggaa gaattgcgcg ccgccgtcga aggtgccgcc aaggctgggt tgccgcttgt
      541 caccaccatg agcttcgaca ccaacggccg caccatgatg ggcatcaccc ccaaggcttt
      601 cggcgcgttg accgcttcgc ttggaacgca acccgtggca atcggcgcca actgcggcgt
      661 gggtgcatcc gaactggtgg caaccgtgct cggcatcagc gaggcccgcc ccgatgcggc
      721 cattgtggcc aagggcaatt gcggcatccc gcaatacaag gaaggccaca tccactacac
      781 cggcaccccg gaattgatgg ccgactacgc ccgcatcgcc ctggactctg gtgcaaagat
      841 catcggcggc tgttgcggta cgtcaccgga acatctggcg gccatgcgga aatccctgga
      901 gagctacacc aagggcccgc gcccctcgat tgaactcgtg gaacagcgcc ttggtccggt
      961 ctcggtcctc gccaaaggca tcgacaccgc cgccgaaggc gccgccaaac gtgaacgccg
     1021 ccggggaaac tagttcgctg gtgcgctcct gctgcctttg accgcgatca acgacgtgga
     1081 cccaacgccg tgctaattta acgcgatgga tcaggccgta ttcttgcgag gcgagacgtg
     1141 gggtttcggc ggacagcccg tcgagcacat tgagactcac gccgcccatg tcttcctagt
     1201 tggtgaccgc gccttcaaga tgaagaaggc ggtcaagctt ccctatctgg atttttctac
     1261 gattgaaaag cgcagggctg tgcttgccca tgaattggag atcaatcgcc cttttgcccc
     1321 ctcggtatat ctgcgcaccg acgaaatccg cggcgagccg gttttggtaa tgaagcggtt
     1381 cgatcagcag gcgctgcttt catcgcaggc gggtggcatc accgatgcaa cggcaaagga
     1441 acttgcccgc atggtggcgg agtcccatgt cattgcgccg cagcgcgaca cgccgggtag
     1501 cgaaatcatg gcggggctgg gcctgcagct ttcgaaagcc tttgccgact cgccggatat
     1561 ttttccggtg gacgaggccg gcaagttcga gctcctccat gaagctcatc tgacgagatt
     1621 gcgtccgctt ctcgatcaga gaagtgcgca agggttggtc cgccgctgcc atggcgacat
     1681 gcattgcggc aatatcgtct tgctggaagg caagccaaac ctctttgacg ccatcgaatt
     1741 tagcgagaaa attgccacca tagatgtgct ttatgatctt gcctttctgc tcatggactt
     1801 ggggcgccgt ggcgagaagc gggtggcgaa cctggtct
//
LOCUS       DAORVN010000496         1833 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_71546_length_1833_cov_2.793588, whole genome shotgun sequence.
ACCESSION   DAORVN010000496 DAORVN010000000
VERSION     DAORVN010000496.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1833)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1833)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1833
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71546_length_1833_cov_2.793588"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1241)
                     /locus_tag="PKV23_09475"
     CDS             complement(<1..1241)
                     /locus_tag="PKV23_09475"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012110191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HQX85707.1"
                     /translation="MARSTFEEFITENKISEVECLVADMSGTARGKILPPKKFMQGQR
                     SRGLRVPEEVFTLTINGRFVSETTAVSDSAIDIYMEPDPETTRMVPWYTEPTAQVICD
                     AFHLNDEPVEISPRHVLKRVLGLYKAKGWRPVVAPELEFYLCKKNLDPDYPIEAAIGR
                     SGRKESGGQAYGIDAANDFDPVVEDIFDYCEAQELDVDTLSHESGPAQLEINFNHGDP
                     LELADQVFLFKRTVRQAALKHDMYATFMAKPHENEPGSSMHIHQSVLDNKTGKNIFAG
                     KDGKPSKLFLNHIGGLQRYLAAALPFCAPNVNSYRRLVPESDAPTNVHWAIDNRTVSL
                     RVPSSNPVNLRVENRVPGADANPYLAFAASLACGYLGMVERIKPSEAVTGSAYRYAHT
                     LPINLDDALDKLNYTKSLKQV"
     gene            1323..>1833
                     /locus_tag="PKV23_09480"
     CDS             1323..>1833
                     /locus_tag="PKV23_09480"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HQX85708.1"
                     /translation="MVKSIDARSYYVETANVQLACENLKGEISADVCVIGAGYTGLSA
                     ALELADAGYKVVVLEAEQIGYGASGRNGGQICTGYSSGQQKIENQLGKADALKCFALA
                     EESKALLQQRVKQHKIDCDLTWGYMHAIPKAHQMEELKAWRDEYEGLGVKGLSLFSKP
                     ELEQRLGTTI"
BASE COUNT          385 a          484 c          577 g          387 t
ORIGIN      
        1 atcacctgct tcagcgactt ggtgtaattc aacttgtcca gggcgtcatc gagattgatc
       61 ggcagggtat gggcatagcg ataggctgaa ccggtcaccg cctccgacgg cttgatgcgc
      121 tcaaccattc ccaggtaacc gcaggcaagt gaggcagcaa acgccaggta ggggttggca
      181 tcggcgccag gcacgcggtt ttccacgcgc aggtttaccg ggtttgaact ggggacgcgc
      241 aggcttacgg tgcgattgtc gattgcccaa tgcacattgg tcggcgcatc cgactccgga
      301 acgaggcggc ggtaggaatt cacattgggc gcgcagaatg gcaaggcggc cgcaagatag
      361 cgctgcagac cgccgatgtg attgaggaac agcttggaag gcttgccgtc cttgccggca
      421 aatatgttct ttccggtttt gttgtccagc accgactggt gaatatgcat ggatgaaccc
      481 ggctcatttt catggggctt ggccatgaag gttgcataca tgtcatgctt cagggccgcc
      541 tgcctgacgg tgcgcttgaa gaggaacacc tgatcggcaa gctccagggg atcgccgtga
      601 ttaaaattta tctcgagctg ggcgggcccg ctttcatggg aaagggtatc gacatccagt
      661 tcctgggctt cgcagtaatc gaagatgtcc tcgacgacgg gatcgaagtc attggcggca
      721 tcgatgccat aggcctggcc gccggattcc ttgcggccgg agcggccaat ggcggcctcg
      781 atcggataat ccggatcgag gttcttcttg cagagataga actcgagctc aggcgccacg
      841 acggggcgcc agcccttggc cttgtaaagc ccgagaaccc gcttcagcac gtggcgcggc
      901 gaaatctcga ccggctcatc attcaagtga aaggcatcgc agatcacctg ggcggtgggc
      961 tcggtgtacc aaggcaccat gcgggtggtt tcggggtccg gttccatgta gatgtcgatg
     1021 gcgctgtcgc tcaccgcggt ggtttccgag acgaagcggc cgttgatggt cagggtaaaa
     1081 acctcttccg gaacccgcag gccgcgcgag cgctggcctt gcatgaattt cttcgggggc
     1141 aagatctttc cgcgtgcggt tccggacata tcggccacca ggcattccac ctcactgatc
     1201 ttgttctcgg tgatgaattc ctcaaatgtc gagcgggcca tgactatcca tgatttgttg
     1261 cgtcaggcca ttgtagcgcg ccttcttgac caagcaaaag ggggtcagct aaagcttttg
     1321 gcatggttaa atcaatcgat gcccggtcct attatgtgga aactgccaat gttcagttgg
     1381 cctgcgaaaa cctcaagggg gagatttccg ccgatgtttg cgtgattggc gcaggctata
     1441 cggggctgtc ggcggccctg gagctggcgg atgcgggcta taaggtcgtg gtgcttgagg
     1501 cggaacagat cggatacgga gcttcggggc gcaatggcgg gcagatatgc accggatact
     1561 cctccgggca gcaaaagatt gaaaaccagc ttgggaaggc cgatgccctg aaatgcttcg
     1621 cccttgccga agaatccaag gccctgctgc agcagcgtgt caagcagcac aagatcgatt
     1681 gcgatctcac ctggggctat atgcatgcca ttccaaaggc ccaccagatg gaggaactca
     1741 aggcctggcg cgatgaatat gaagggctag gggtaaaagg cctgagcctg ttcagcaagc
     1801 ccgaacttga acagcggctg ggcacgacga tct
//
LOCUS       DAORVN010000497         1831 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_71684_length_1831_cov_2.737050, whole genome shotgun sequence.
ACCESSION   DAORVN010000497 DAORVN010000000
VERSION     DAORVN010000497.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1831)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1831)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1831
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71684_length_1831_cov_2.737050"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..759
                     /locus_tag="PKV23_09485"
     CDS             <1..759
                     /locus_tag="PKV23_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003525573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQX85709.1"
                     /translation="SYALYPHMSVRGNISFGLKMAGMPKAEIEAKVAKAAATLNLTEY
                     LDRKPRQLSGGQRQRVAIGRAIVRNPEGFLFDEPLSNLDAALRVNMRLEIMQMHKDLG
                     ATMIYVTHDQVEAMTMADRIVVLNRGNIEQVGSPLELYNRPDSLFVAGFIGSPKMNLI
                     SGKPAEKFDAHTIGIRPEHLGISLKDGTWSAKISLAEHLGADTFLHVDAGPCGMMTVR
                     AAGEYDAAPGAKIFLTPEPDRIHRFNKDGRAIRK"
     gene            798..1574
                     /locus_tag="PKV23_09490"
     CDS             798..1574
                     /locus_tag="PKV23_09490"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006611404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HQX85710.1"
                     /translation="MYLEKLKLDGQVAVVTGAGQGIGAACARALGEAGAKVIVADFMP
                     NRIETAVAELRKLKIDAHGAKLDVTKSKEVDAVCADILKAHGRVDILVNNAGVAKSDV
                     RAEDTSDDHWRHHMDVNVDGLFWCCRAFGGVMLKQGKGAIVNIGSMSGFIVNKPQPQS
                     FYNASKAAVHHLTRSLAAEWATRGVRVNAVAPTYIETPLTAFGIKESPEMYKTWLEMT
                     PMGRVGQPDEIASVVHFLASDAASLMTGSIVLADGGYCCW"
     gene            complement(1580..>1831)
                     /locus_tag="PKV23_09495"
     CDS             complement(1580..>1831)
                     /locus_tag="PKV23_09495"
                     /inference="COORDINATES: protein motif:HMM:NF019643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BBE domain-containing protein"
                     /protein_id="HQX85711.1"
                     /translation="VMVSFIAVYGDPGEQAVHDRWAADGIAELPQEMDRVYVNFLLAD
                     PAERIHAAYPPQTLERLKLVKRRYDPENLLRLNRNVTPA"
BASE COUNT          381 a          578 c          528 g          344 t
ORIGIN      
        1 tcctatgcgc tttatccgca catgagtgtt cgcggcaaca tctccttcgg cctcaaaatg
       61 gccggcatgc ccaaggccga gatcgaagcc aaggtggcca aggccgccgc cacgcttaat
      121 ctcaccgaat atctcgaccg caagccgcgc cagctttccg gcggccagcg ccagcgtgtt
      181 gccatcggcc gcgccatcgt gcgcaatccc gagggcttcc tgtttgacga gccgctctcc
      241 aatcttgacg ccgcacttcg cgtcaacatg cgcctggaaa tcatgcagat gcacaaggat
      301 ctgggcgcca ccatgattta cgtgacccat gaccaggtgg aagccatgac catggccgat
      361 cgtattgtcg ttttgaaccg ggggaatatc gaacaggtgg gctctccact tgaactctac
      421 aacagaccag actctctctt cgttgccgga tttatcggtt caccaaaaat gaacctgata
      481 tccggaaagc cagccgaaaa gtttgacgcc cataccattg ggatccgtcc cgagcatctc
      541 ggcatctcat tgaaggatgg cacctggtcc gcgaaaattt cgcttgcgga acatctcggc
      601 gccgatacgt tccttcacgt cgatgcgggg ccctgcggca tgatgacggt gcgcgctgcc
      661 ggcgaatatg acgctgcccc cggcgcgaag atcttcctca cgccggagcc tgaccgcatt
      721 caccgcttca acaaggatgg aagggccatc cgcaaatgag ctttgcccaa taggaaacca
      781 gttaggaaaa cgagaaaatg tatctggaaa aactcaaact cgacggacag gttgccgtgg
      841 tcacgggcgc cgggcagggc attggtgcgg cctgcgccag ggccctgggc gaagccggcg
      901 caaaagtcat cgtcgccgac ttcatgccca accgcattga gacggccgtg gccgaattgc
      961 gcaagctgaa gattgatgcc catggcgcca agctcgatgt gaccaagtcc aaggaagtgg
     1021 acgccgtctg cgccgacatc ctcaaggccc acggccgcgt cgatatcctg gtaaacaatg
     1081 ctggcgtcgc caaaagcgat gtacgcgccg aagacaccag cgatgaccac tggcgccacc
     1141 acatggatgt gaacgtcgat ggcctgttct ggtgctgccg cgcctttggc ggcgtgatgc
     1201 tcaagcaagg caagggcgcc atcgtgaaca tcggttccat gtcgggcttc atcgtcaaca
     1261 agccccagcc gcaatccttc tacaatgcct cgaaggccgc cgtacatcat ctcacccgct
     1321 cgctggcagc cgaatgggcc acccgcggcg tgcgcgtcaa tgcggtggcg cccacctata
     1381 ttgaaacccc gctcaccgcc tttggcatca aggaatcgcc cgagatgtac aagacgtggc
     1441 tggaaatgac gcccatgggc cgcgtcggcc agcccgacga aatcgcctcg gtcgtgcatt
     1501 tcctcgcctc cgacgccgcc agcctgatga ccgggtcgat tgtcctggct gacggcggct
     1561 actgctgctg gtagcacggt tacgctgggg taacgttgcg gttcaggcgg agcaggtttt
     1621 ctggatcgta ccggcgcttg accagcttca gccgctccaa tgtctggggc ggataggccg
     1681 catgaatgcg ctcggccgga tcggcgagca ggaaattgac gtataccctg tccatctcct
     1741 ggggcagttc cgcaatgccg tcggcagccc agcggtcgtg cacggcctgc tcgcccgggt
     1801 cgccgtagac ggcgatgaac gaaaccatga c
//
LOCUS       DAORVN010000498         1825 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_72099_length_1825_cov_4.177966, whole genome shotgun sequence.
ACCESSION   DAORVN010000498 DAORVN010000000
VERSION     DAORVN010000498.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1825)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1825)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1825
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72099_length_1825_cov_4.177966"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="PKV23_09500"
     CDS             <1..261
                     /locus_tag="PKV23_09500"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQX85712.1"
                     /translation="KKFDLAVMDVEMPELDGLETTRRVRAMEKKSGSASHLPILALTA
                     NARRENYLECMAAGMDGHLSKPFDRQDMEEAIAKILSLKTAA"
     gene            329..1168
                     /locus_tag="PKV23_09505"
     CDS             329..1168
                     /locus_tag="PKV23_09505"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acyltransferase"
                     /protein_id="HQX85713.1"
                     /translation="MDRLSPEQDLLGTRNDLCVRLARSEREVATAQELRFSVFYEELK
                     ASPDTAASHSRRDADRFDAFCDHLIVVRRTAGNEAGKFGVDDGEMVGTYRLLRQSVAE
                     QNGGFYTQSEFDIAPLLAAKPALKFLELGRSCVLKQYRTKPVVELLWQGIWNYVRHHG
                     MDVMLGCASLEGTDHRVHAPVLDFLARTCAAPPEWRVRAVSEVQIEMNSSAHAPIDGK
                     AALKALPPLIKGYLRLGCFIGDGAVIDRQFNTTDVLIILPVSNINPRYFAHFGQPDET
                     VTY"
     gene            complement(1158..>1825)
                     /locus_tag="PKV23_09510"
     CDS             complement(1158..>1825)
                     /locus_tag="PKV23_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020048203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HQX85714.1"
                     /translation="NQDRNVFASCMVALGDADALVTGLTRNFSVALDNVRHAIDNRPG
                     HRPIGVSMALVRGRTVFIADTSIHELPTAEELADIAQEAAGVARRFGYEPRVAFLSYS
                     TFGYPRGERAEYVRNAVAVLDKRNVDFEYDGEMAADVALSRDAMALYPFCRLTAPANV
                     LVMPAAHSASISTKMLQQLGGITVVGPLLTGLAKPVQIASMSATTTDIVNLAAIAAFD
                     INR"
BASE COUNT          383 a          523 c          573 g          346 t
ORIGIN      
        1 aaaaaattcg atctggcggt gatggacgtt gagatgccgg aactggatgg gctggaaaca
       61 acccggagag tccgcgcaat ggaaaagaaa tcaggctccg cctcgcatct gcccattctg
      121 gcgctcacgg ccaacgcacg ccgggaaaat tatctggaat gcatggcggc aggcatggac
      181 gggcatcttt ccaagccctt tgaccggcag gacatggaag aggcaatcgc gaaaatcctg
      241 agcttgaaga ccgccgcctg atcccgtcac caaactttca tattcttctt gattatggca
      301 gtttgccttg atgatgcagg agtcgatatt ggaccgtctc tcacctgagc aggatctgct
      361 ggggacgcgt aatgacctgt gtgtccgcct ggcgcgaagc gaacgggaag ttgccacggc
      421 gcaggaattg cggttcagcg ttttctatga ggaactgaag gcgagccccg acactgcggc
      481 ttcacattcg cgccgggatg cggaccgttt cgacgcattc tgcgatcacc tgattgtagt
      541 tcgcagaacg gcggggaatg aagctggaaa attcggcgtt gatgacggtg aaatggtggg
      601 cacctaccgc ctgctgcgcc aatccgtggc ggagcagaac ggcggtttct acacgcaaag
      661 cgaattcgac atcgcgccgc tgcttgcggc aaagcccgcc ctgaagtttc tcgagctggg
      721 aaggtcctgt gtgctcaaac aataccggac aaagcctgtg gtcgagttgc tctggcaggg
      781 catctggaac tacgtaaggc accatggaat ggatgtcatg ctgggctgcg ccagccttga
      841 gggaactgat cacagggttc atgcgcccgt actggatttc cttgcccgca cttgcgcggc
      901 tccgccggaa tggcgcgtgc gcgcagtgtc tgaagtccag attgagatga actcaagtgc
      961 ccatgccccc atcgatggca aggcggcgct gaaggccctg ccgccgttga tcaagggcta
     1021 cctgcgcctg ggctgcttta tcggcgacgg ggcggtgatc gaccggcagt tcaacaccac
     1081 cgacgtgctc attatcctgc ccgtttcaaa catcaatccg cgttactttg cgcatttcgg
     1141 ccagcccgac gaaaccgtca cctattgata tcaaaagcgg cgattgccgc cagattgacg
     1201 atgtcggtcg tggtggccga catggaggcg atctgcaccg gctttgccag cccggtcagc
     1261 aatggcccca caaccgtgat gccgccaagc tgctggagca ttttggttga aatcgaagcg
     1321 gaatgggccg ccggcatcac caggacattc gccggcgcgg tcaggcggca aaacggatag
     1381 agcgccatgg catcgcgcga aagcgccaca tcggccgcca tctcgccatc atactcaaaa
     1441 tcgacattgc gcttgtcgag cacggccacg gcattgcgca cgtattcggc gcgctcgccg
     1501 cggggatagc caaaggtgga ataggacagg aaggccacgc gcggctcata gccaaagcgc
     1561 cgggcaacgc ccgcggcttc ctgcgcgatg tcggccagtt cctctgcggt cggcagttcg
     1621 tggatcgagg tatcggctat gaacacagtc cgcccgcgga cgagggccat cgacacgccg
     1681 atggggcggt gccccgggcg gttgtcgatg gcgtggcgca cgttgtcgag ggccacggaa
     1741 aaattccgcg tgaggccggt gacgagcgca tcggcatcgc caagagcgac catgcaggag
     1801 gcgaagacgt tgcggtcctg gttca
//
LOCUS       DAORVN010000499         1811 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_73031_length_1811_cov_2.884396, whole genome shotgun sequence.
ACCESSION   DAORVN010000499 DAORVN010000000
VERSION     DAORVN010000499.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1811
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73031_length_1811_cov_2.884396"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1104)
                     /gene="dnaA"
                     /locus_tag="PKV23_09515"
     CDS             complement(<1..1104)
                     /gene="dnaA"
                     /locus_tag="PKV23_09515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HQX85715.1"
                     /translation="MAERPAEKPRPAQDEPRGSAQPGANFLQQGERNTQLDSTQTFDS
                     FVVGTSNQLAHAAACRVTEASAGAPVTFNPLFIHSASGLGKTHLLNAIGHRIRDIHPE
                     RKVLFLTAERFMYSFIQALRSRDTISFKDQFQSVDVLLIDDFQFLQGKTMQQEFCHTF
                     NSLVDSRRQVIVAADVPPSQLDTIDQRMRSRLMGGLVIDIELPDLELRRKIVLGRYVA
                     MNSRDHSVVIPDDVLEFVANRITGGGRELEGALNRIVAYQQFNKSPITLDLAAMVLRD
                     MSANPDQCRIKIDDILKIVGRHFNVARTDLLSPRRARSIVVPRQVGMYLAKKMTSRSL
                     PEIGRRFGGRDHSTVLHAVRKIDDQIKTDDRLAK"
     gene            complement(1105..1398)
                     /locus_tag="PKV23_09520"
     CDS             complement(1105..1398)
                     /locus_tag="PKV23_09520"
                     /inference="COORDINATES: protein motif:HMM:NF023069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA N-terminal domain-containing protein"
                     /protein_id="HQX85716.1"
                     /translation="MTDLAVETVDLKQKWQRVSHRLRAELGDDLFTSWFGRMDAEDCC
                     NLKLIVSVPTRFLKSWIENHYVTKLQKIAETEFGRLDAVQVRVRGQVTPPRAV"
BASE COUNT          365 a          504 c          564 g          378 t
ORIGIN      
        1 cttggccagg cggtcatcgg tcttgatctg gtcatcgatc ttgcgcaccg catggagcac
       61 ggtggaatga tcgcggccgc cgaacctgcg gccgatttcg gggagcgagc gcgaggtcat
      121 tttcttcgcg agatacatgc cgacctggcg cggcacgaca atggagcggg cgcggcgcgg
      181 cgaaagcaga tcggtgcgcg ccacgttgaa atggcggccg acgattttca ggatgtcgtc
      241 gatcttgatg cggcactggt cgggattggc cgacatgtcg cggagcacca tggcggcgag
      301 gtcgagggtg atgggcgatt tgttgaactg ctgataggca acgatgcggt tcagggcgcc
      361 ttcgagctcg cggccgccgc cggtgatgcg gttggcgaca aattccagga cgtcatcggg
      421 gatgaccacg gaatgatcgc ggctgttcat ggcgacgtag cggcccaaga caatcttgcg
      481 gcgcagttcg aggtcgggca gctcgatgtc aatgaccagg ccgcccatga gcctcgagcg
      541 catgcgctgg tcgatggtat cgagttgtga cggcggaacg tccgccgcca caattacttg
      601 cctcctcgaa tccaccaacg agttaaacgt atggcagaac tcttgctgca tggttttgcc
      661 ctgcaggaac tggaaatcgt caatgagcag cacatccacc gactggaact ggtccttgaa
      721 ggagatggtg tcgcggctgc gcagggcctg gatgaagctg tacatgaagc gctcggcggt
      781 gaggaacagc accttgcgtt cgggatgaat gtcgcggatg cggtggccga tggcgttgag
      841 caaatgggtc ttgcccagcc ccgaggcgga atgaatgaac aggggattga aggtgacggg
      901 agcgccggca ctggcctcgg tcacccggca ggcggcggca tgggccagct gattggaggt
      961 tcccaccacg aaactgtcaa aggtctgggt tgaatcaagc tgggtattgc gctcgccctg
     1021 ctgcaggaaa ttggcgccgg gttgggcgct gccgcgcggc tcatcctggg cggggcgggg
     1081 cttttcggcg gggcgctcgg ccatttagac ggcgcggggc ggcgtcacct ggccgcggac
     1141 ccgcacctgc accgcatcaa gccggccaaa ttcggtttcg gcaattttct gcagcttggt
     1201 tacatagtgg ttttcaatcc agctcttgag gaagcgggtg ggcacggaga caatgagttt
     1261 caggttgcag caatcttccg cgtccatgcg gccaaaccag ctggtaaaga gatcatcccc
     1321 caattcggcc cggagccggt gggaaacccg ctgccacttc tgtttcagat cgaccgtctc
     1381 aaccgctaaa tcagtcattt tcccgcgcac tctctcgttg cattcttcaa ctcataattc
     1441 ccgtgaagct tcagacacag gaattcggca tcgttcctgt caaaaagaac aatccacgcc
     1501 tgtgcgacgc ctcagaccta caacttggag aacctacccg cccttaaacg cccaaataac
     1561 tccgtgtcag aaacaaaccc cacactctga catttcattt tttgcatccc tttggtgttt
     1621 taacccgctg tcttcgtgcc ttcccgtgag cagcgttgag gagaaactac aaaagccaag
     1681 cgacacactg caagatgaat caggcattgt tccgtacacg gagcgcaccg gtggtttggg
     1741 cggttttcga gcgccccttc ggagttttgc taactctatg atgtgacatt gtgaatcagg
     1801 ccgatccggg c
//
LOCUS       DAORVN010000500         1811 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_73057_length_1811_cov_2.254556, whole genome shotgun sequence.
ACCESSION   DAORVN010000500 DAORVN010000000
VERSION     DAORVN010000500.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1811
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73057_length_1811_cov_2.254556"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="PKV23_09525"
     CDS             <1..327
                     /locus_tag="PKV23_09525"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQX85717.1"
                     /translation="IVGFVSCGAAREGAAKGADGEILAIYILKAYHGRRIGRSLMAAA
                     ARFWLSKGGRNLVVLSMAGNNQAAAFYEALGGLRVDEGSFEIAGTRIDDTGHLFNNLA
                     ELAALP"
     gene            421..708
                     /locus_tag="PKV23_09530"
     CDS             421..708
                     /locus_tag="PKV23_09530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85718.1"
                     /translation="MAEHSSGNHAQDMEAHRSTYSAFVTGSIALSLVCAFVLVSLVSF
                     RFAGTLNVFMGFAGLIVGIVAVLIDVRTGGKWLLSLGALVLFGLITAINVS"
     gene            721..>1811
                     /locus_tag="PKV23_09535"
     CDS             721..>1811
                     /locus_tag="PKV23_09535"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009398973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HQX85719.1"
                     /translation="MKLAVLSETSPGERRVSASPDTVKAYVKKGLAVVVQAGAGAGAH
                     ISDEAFVAAGASIIKSDAGLKDADVVLAVRRPSDAILKSLKKGAVVAAGLDPYGERKG
                     LEALAKSGQSFFAMELMPRISRAQSMDILSSQSNLAGYKAVIDAAAFFGRAFPMMMTA
                     AGTVPPAKVFIMGVGVAGLQAIATARRLGAIVSATDVRKATEEQVKSLGAKFVFADVA
                     DAAAAGGYAKELSAEDKAKQAALVAEHIKGQDIVITTALIPGRPAPVLVSKDMVESMK
                     PGSVIVDLAVERGGNCPLSKPDQVVEHKGVSIIGTVNLAGKLAGNASPLFAKNLANFL
                     DLMIQPDGSLKIDYEDEIIKGTLIARDGAL"
BASE COUNT          320 a          495 c          610 g          386 t
ORIGIN      
        1 atcgtgggat ttgtttcgtg tggcgcggcg cgcgaaggcg cggccaaggg cgccgatggc
       61 gaaattctcg cgatctatat tctcaaggct tatcatggcc ggagaatcgg gcgcagcctc
      121 atggccgcgg cggcccggtt ctggctttca aaaggcgggc gcaatcttgt cgtcctgtcc
      181 atggcgggca acaatcaggc cgcggccttt tacgaagctc tgggcgggct tcgggtcgat
      241 gagggcagtt ttgagattgc ggggacccgg attgacgata cggggcacct tttcaacaat
      301 ttggctgaac ttgcggccct tccgtgactc atcgccgcgt ttggttgggg tgcttgcagg
      361 gcgttcccgg cttggctaca catcttgcag agattcatga cgtttgaggg gacttcacca
      421 atggccgagc attcttcagg caatcacgca caggacatgg aagcgcatcg cagcacctac
      481 agcgccttcg taacgggctc cattgcgctt tcactagtct gcgcttttgt gctggtgtcc
      541 ctggtttcat tccgctttgc cgggacgctc aacgtgttca tggggtttgc cggcctgatc
      601 gtcgggatcg tggccgtgct gattgacgtg cgcaccggcg gcaagtggct gttgtccctt
      661 ggcgcgctgg tgctgttcgg gctgatcacc gccataaacg tttcataggc cgggcgaaat
      721 atgaagctcg cggtcctttc tgaaacgagt cccggagagc gccgggtttc ggcaagcccg
      781 gacacggtca aggcctatgt gaaaaaagga cttgctgttg tggtgcaggc aggcgcaggt
      841 gctggggcgc atatttccga tgaggctttt gtggccgcgg gcgccagcat catcaaatcc
      901 gatgcagggc tcaaggacgc cgatgtcgtc ctggcggtgc gccggccatc ggatgccatt
      961 ctcaaaagct tgaaaaaggg cgcggtcgtg gctgcggggc ttgatcctta cggcgagcgc
     1021 aaggggcttg aggcgctggc caagtcgggc cagtcatttt ttgccatgga actcatgccg
     1081 cgcatttcgc gggcccaatc catggatatc ctgtcatcgc aatccaatct cgctggctac
     1141 aaggcagtga tcgatgcggc cgcctttttc ggccgggcct tcccgatgat gatgacggcg
     1201 gcgggcacgg ttccgccggc caaggtgttc atcatgggtg tgggcgttgc ggggctgcag
     1261 gcgatagcca cggcgcggcg cctgggggcg attgtttcgg caaccgacgt gcgcaaggcg
     1321 accgaagaac aggtgaagag ccttggcgca aagtttgtct tcgccgatgt ggccgatgcg
     1381 gcggctgccg gcggctatgc caaggaactc tcggcggagg acaaggccaa gcaggcagcc
     1441 cttgtggccg agcatatcaa gggccaggac attgtgatca ccacggcgct cattccgggt
     1501 aggccggcgc cggtgctggt gagcaaggac atggtcgaga gcatgaagcc cggttcggtg
     1561 attgtcgatc ttgcggtgga gcgcggcggc aattgcccgc tttcgaagcc agaccaggtt
     1621 gtcgagcaca agggcgtttc gatcatcggc acggtgaacc tggcgggcaa gctggcgggc
     1681 aatgcctcgc cgctgtttgc caagaacctg gccaatttcc tcgacctgat gatccagccc
     1741 gatggcagcc tgaagattga ctatgaagac gagatcatca agggcacgct gattgcccgg
     1801 gatggcgcgc t
//
LOCUS       DAORVN010000501         1809 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_73159_length_1809_cov_3.595211, whole genome shotgun sequence.
ACCESSION   DAORVN010000501 DAORVN010000000
VERSION     DAORVN010000501.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1809
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73159_length_1809_cov_3.595211"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..401
                     /locus_tag="PKV23_09540"
     CDS             <1..401
                     /locus_tag="PKV23_09540"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HQX85720.1"
                     /translation="IAALPFYDNPEGGVDVCLVTARGSGRWIIPKGNPIRGLAPHEVA
                     AREAFEEAGLVGHVGKCCIGTFKFERSRNGRDATCRVDVYALRVERQMQTWTEMHERS
                     VLRCNVKTALSLVSVPNLATLINRYAMAHV"
     gene            complement(409..762)
                     /gene="zapA"
                     /locus_tag="PKV23_09545"
     CDS             complement(409..762)
                     /gene="zapA"
                     /locus_tag="PKV23_09545"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HQX85721.1"
                     /translation="MANVVVSVADRPYTMQCPDGEEDHLRELASLLDAEIVRIKQSVG
                     SIGDIRLLVMSGLMVADRLSEAIRKIEALEDQIKGLRESRNLAQAQTKDLEAKFAARL
                     ESASARLESIAKAMK"
     gene            complement(766..1029)
                     /locus_tag="PKV23_09550"
     CDS             complement(766..1029)
                     /locus_tag="PKV23_09550"
                     /inference="COORDINATES: protein motif:HMM:NF025122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4164 family protein"
                     /protein_id="HQX85722.1"
                     /translation="MVDTQKLNDAFDRFDAALKTFEGALAKKQAIASEAEALRGDRAK
                     IASELDLVRNKATELVTTNKSAVGKIDAAMSRIRAVLHSNSGG"
     gene            1309..>1809
                     /locus_tag="PKV23_09555"
     CDS             1309..>1809
                     /locus_tag="PKV23_09555"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002970273.1"
                     /note="catalyzes the formation of ribose 5-phosphate and
                     xylulose 5-phosphate from sedoheptulose 7-phosphate and
                     glyceraldehyde 3-phosphate; can transfer ketol groups
                     between several groups; in Escherichia coli there are two
                     tkt genes, tktA expressed during exponential growth and
                     the tktB during stationary phase; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HQX85723.1"
                     /translation="MTVPSLDMQKTMANAIRALSMDAVQKANSGHPGLPMGAADFATV
                     LFTQFLNFDASDPHWPDRDRFILSAGHGSMLLYSLLFLTGYKDMTLEQLKNFRQLGSI
                     TAGHPEYGHAEGIETTTGPLGQGLANAVGFAIAERHLAARFGNDLVNHKTYVLAGDGC
                     LMEGISQ"
BASE COUNT          359 a          583 c          491 g          376 t
ORIGIN      
        1 agattgccgc tctgccattt tatgacaacc cggagggtgg tgtggacgtc tgcctagtga
       61 ccgccagggg cagcgggcgc tggatcatcc caaaaggcaa tcccatccgc ggcctcgccc
      121 cccatgaggt tgcagcccgc gaagccttcg aggaggcggg gcttgttggc catgtgggca
      181 agtgctgtat cggaactttc aaattcgagc ggagccggaa cgggcgcgac gccacctgcc
      241 gagtcgatgt ctatgccctc agggttgaac ggcagatgca gacctggacg gaaatgcatg
      301 aaagatcggt gctgcgctgc aatgtaaaaa ccgcgctctc gcttgtcagc gtgccaaacc
      361 tcgccaccct gatcaaccgc tacgccatgg cccacgttta ggggaaggct acttcatcgc
      421 cttggcaatg ctctccagcc gggccgaggc gctttccagc cttgcggcga attttgcttc
      481 caggtccttg gtctgcgcct gggccaggtt ccgcgattca cgcaggccct tgatctggtc
      541 ttccagcgcc tcgatcttgc ggatcgcttc cgacagccgg tccgccacca tcagcccgct
      601 catcaccagc aggcggatat cgccgatcga tccgacgctc tgcttgatcc gcacgatctc
      661 cgcatcgagc agcgaggcaa gctcgcgcaa atggtcttcc tcaccatcgg ggcactgcat
      721 cgtataagga cgatcagcaa ccgagacgac aacattggcc attcctcagc ccccactgtt
      781 gctgtgcagc acagcgcgaa tgcgtgacat ggccgcgtcg atttttccga cagccgattt
      841 gttggtggtg acgagttccg tggccttgtt gcgcacgaga tcgagttccg aggcaatctt
      901 cgcgcggtcg ccgcgcagcg cctcggcctc gctggcaatc gcctgcttct tggccagcgc
      961 cccttcgaat gttttcaacg ccgcgtcgaa acgatcaaag gcatcgttta gtttttgtgt
     1021 atcaaccatg tccgtcccta gatcacgttt cgcctcagga caacagcctg aagccataaa
     1081 cataaccgcc acctgttttc cggcgcgtga tcggacacta atactggtct cacgctgccc
     1141 aaccacacgc tggcaaatca actaatttgc cgcctttgcc aatatgttac gtaaacaaac
     1201 gcacttcaat agcttaattt gccgcgataa gccgctggaa aagtgcggtt gacagggcgg
     1261 gaactgctgt taatcagaag cccgattgcg gcaaggattt ccccgacaat gactgttccc
     1321 tccctcgaca tgcagaaaac catggccaat gccatccgcg cgctgagcat ggacgccgtc
     1381 cagaaggcaa actccggcca tcccggcctg cccatgggtg cggcggattt tgccacggtc
     1441 ctgttcaccc agttcctcaa ttttgacgcc agcgatcccc attggccgga ccgcgaccgc
     1501 ttcatactct ccgccggcca cggctccatg ttattgtatt cactgctgtt tctgacgggc
     1561 tacaaggaca tgaccctgga gcagctcaag aacttccggc agttgggttc cattaccgcg
     1621 ggccacccgg aatatggcca tgccgagggc attgaaacca ccaccggccc cctgggccag
     1681 ggccttgcca atgccgtggg ctttgccatt gccgaacgcc acctggcggc ccgcttcggc
     1741 aacgacctcg tcaatcacaa gacctatgtg ctggcgggcg acggctgcct catggaaggc
     1801 atcagccag
//
LOCUS       DAORVN010000502         1808 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_73209_length_1808_cov_4.278380, whole genome shotgun sequence.
ACCESSION   DAORVN010000502 DAORVN010000000
VERSION     DAORVN010000502.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1808
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73209_length_1808_cov_4.278380"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..711
                     /locus_tag="PKV23_09560"
     CDS             <1..711
                     /locus_tag="PKV23_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018183925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HQX85724.1"
                     /translation="GFVVTQWNKPDDLHPWDFDPNYVAHMSFDGVPTAEAIANELFKA
                     FGGEGGIVALGGILSNVPAIERKLGLDNAIAKTNGKVTLLDFQPADWNEQKAFDITQA
                     WLTRFGDQVKGVFAANDGMGMGALEALRQNGLAGKVPVVGIDGIDAAVTAIEAGEFAG
                     TVAWDPIWTGGMGLAIPYAHATGKIDITKEPREHREFYGTGIIVSKDTVADYKNNPPK
                     VDYNDLWGKATGQIQYRS"
     gene            786..1763
                     /locus_tag="PKV23_09565"
     CDS             786..1763
                     /locus_tag="PKV23_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008124693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQX85725.1"
                     /translation="MKPVSVSNGNLLGHWRRWAPVVVLLALCILISLFNGNFLTFGNF
                     IRLLNSAAIPIVLCMGATFIILMGSIDLSIEGVVALTAVIVSLLVINDISPYALSLWA
                     VPIAVAVGGMMGLLNGVLHIKLKTPSFMTTLGVGFAGVGVATTILGGTTVRISDQTFR
                     FLSLGRVLEIPMAVWIAAVAVAVAYVIQERTRLGRWLYAIGTDEMTARHAGIPIERTR
                     ILIFGIAGLFYGLGGVLAAAQFGQGHALISQGRLFTTITAVVVGGTALSGGVGSVINS
                     VIGVFIVVVLANGLVLMGVEPYVQQGVQGLLIIAAVALALDRSRLDVVK"
BASE COUNT          327 a          533 c          539 g          409 t
ORIGIN      
        1 ggcttcgtcg tcacccagtg gaataagccc gacgatctgc atccctggga cttcgacccg
       61 aattatgtgg cccacatgtc ctttgacggc gtgccgacgg ctgaggccat cgccaacgag
      121 ctgttcaagg cattcggcgg cgagggcggc atcgtggcgc tgggcggaat tctgtccaac
      181 gtccccgcga tcgagcgcaa gctcggcctc gacaacgcca ttgccaagac caacggcaag
      241 gtgactctcc ttgacttcca gcctgccgac tggaacgagc aaaaggcctt cgacattacc
      301 caggcctggc tcacgcgctt tggcgatcag gtcaagggcg tgttcgccgc caatgacggc
      361 atgggcatgg gcgctctcga agcccttcgc cagaacggcc tggcaggtaa agtgccggtc
      421 gttggaattg acggcattga cgctgccgtc acggccatcg aagcaggtga attcgcgggc
      481 acggttgcct gggatcccat ttggactggc ggcatgggcc ttgccattcc ctatgcccac
      541 gccacaggca agatcgacat caccaaggaa ccccgcgaac accgcgaatt ctatggcacc
      601 ggcataatcg tgtccaagga tacggtggcg gactacaaga acaatcctcc caaggtggac
      661 tacaacgatc tctggggcaa ggcgaccggc cagatccagt atcgcagctg aacttccgca
      721 atgacctatg gccgggtgcc tggcacccgg ctaatctatt ggtcccttaa gggcgggaga
      781 acgctgtgaa gccggtttca gtgtcaaacg gaaatctcct tgggcattgg cgcagatggg
      841 cgccggtcgt ggtgctgctt gcgctgtgca ttctcatcag cctgttcaac ggtaatttcc
      901 tcaccttcgg caacttcatc cggctgctga actcggctgc gattcccatc gtgctttgca
      961 tgggggctac gttcatcatt ctcatgggta gcattgatct gtccattgaa ggtgtggtgg
     1021 cgctgactgc ggtgattgtg agcctcctgg ttataaatga catatcgcct tatgcgctaa
     1081 gcctctgggc cgtgcccatt gccgtggctg tgggcggtat gatggggctg ctcaacggcg
     1141 ttctccacat aaaactgaaa actccatcct tcatgacaac gcttggtgtg ggcttcgctg
     1201 gagttggtgt cgccacgacg attctgggcg gcaccacagt ccgtatatca gaccagacct
     1261 ttcgctttct ctcccttggc cgcgtattgg aaatcccgat ggccgtatgg attgccgccg
     1321 ttgcagtggc ggttgcctat gtcattcagg aacgcacgcg cctggggcgc tggctttatg
     1381 ccatcggcac ggatgaaatg acggcgcgcc acgccggcat tcccatcgaa cgcacacgga
     1441 tcctcatttt cggcattgcc ggcctgtttt atggcctggg cggggtgctt gccgcggcgc
     1501 aatttggcca gggccatgcc ctgatcagtc agggccggct cttcaccacg attaccgccg
     1561 ttgttgtcgg cggaacggcg ctctccggcg gcgttggctc cgtaatcaat tcggtcattg
     1621 gcgttttcat cgtcgttgtg ctggccaatg gcctggttct gatgggggtc gagccttatg
     1681 tgcagcaagg cgtgcagggc ctgctgatca ttgccgccgt ggcgctggcc ctcgatcgtt
     1741 cgcgccttga tgtggtgaaa tgatgcagcc agtccttgaa gtccgcaatg tctcgaaatc
     1801 cttccccg
//
LOCUS       DAORVN010000503         1808 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_73254_length_1808_cov_2.369082, whole genome shotgun sequence.
ACCESSION   DAORVN010000503 DAORVN010000000
VERSION     DAORVN010000503.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1808
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73254_length_1808_cov_2.369082"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..347)
                     /locus_tag="PKV23_09570"
     CDS             complement(<1..347)
                     /locus_tag="PKV23_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019965656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HQX85726.1"
                     /translation="MSSSFGIALLILAIMAAIGAPIAFAMVVSAVTYLLLSGQDLALS
                     SEQFIQSVYDSFVLLAVPLFITAANIMNVGAISDRLLEFCKAIVGRFKGGMAQVDILV
                     SLIFSGMSGSAVA"
     gene            complement(344..865)
                     /locus_tag="PKV23_09575"
     CDS             complement(344..865)
                     /locus_tag="PKV23_09575"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HQX85727.1"
                     /translation="MKPLTQWLRHAAEGVAAAMLVALFAVFLVQIAARYVFNVPMGWT
                     VEVCLTLWLWIVFWGGAFCLRPADHIRFDVLYLSVNRPTQRIFGAIAAVMIVAAFAVA
                     FLPTFDYVAFYKIKRSNTLGIRLHYVFSIYLLFMLVVILRYSGVLLNFLRGKEEVEGK
                     VSAYTRHDEGSIT"
     gene            complement(887..>1808)
                     /locus_tag="PKV23_09580"
     CDS             complement(887..>1808)
                     /locus_tag="PKV23_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006725145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sialic acid TRAP transporter substrate-binding
                     protein SiaP"
                     /protein_id="HQX85728.1"
                     /translation="LAQDKLKLRLSSVNSVTDQRAIALIEKFGPAIADFATFEPYWGG
                     SLFKQGTELEAISRGNIEMSISSAQEIANFFPEFSVFSAGYLHRDAAHQVAVFNAPLM
                     QPFKDKIETELGVKLLSVMYLGRRQINLRTDKEIRTPADLAGIKLRMPPSDAWQFLGK
                     ALGANPVPVPFPELYTALQTGSVDGQDNPLPTVMDAKYYEVTKQIVLTSHLVDLNYLT
                     ISKKAWDSLSPENQAKLQKAADEACEFGRQNQLKKEAELVEGLKAKGLKIYEPDIEAF
                     RTTVQKAYLESDFSKTWPAGVVDAVNALAK"
BASE COUNT          387 a          509 c          582 g          330 t
ORIGIN      
        1 tcggcgacgg cggagcccga catgccggaa aagatcaggc tgaccaggat atcgacctgc
       61 gccatgccgc ccttgaagcg gccgacgatg gccttgcaga attcaagcag ccggtcgctg
      121 atggcgccga cattcatgat gttggcggcc gtgatgaaga gcggcacggc gagcaggaca
      181 aagctgtcat agacgctttg aatgaactgt tccgaggaga gcgccaggtc ctggcccgag
      241 agcaggagat aggtcacggc cgagaccacc atggcgaagg cgatgggcgc gccaatggcc
      301 gccatgatgg cgagaatgag aagcgcgatg ccgaaggaag aactcacgta atgcttccct
      361 catcgtggcg ggtgtaggcc gataccttgc cctccacctc ctccttgccc cgcaggaaat
      421 tgagcagcac gccggaatag cgcaggatca ccaccagcat gaacaggagg tagatggaaa
      481 atacgtaatg caggcgtatc cccagcgtgt tgctgcgctt gatcttgtag aaggccacgt
      541 aatcgaaggt tggcaggaaa gccacggcaa aggcggcgac gatcatgacg gccgcaatgg
      601 cgccgaagat gcgctgcgtc gggcgattga ccgaaaggta aagcacgtca aagcggatgt
      661 ggtcggcggg gcggaggcag aaggccccgc cccagaaaac gatccacagc cagagcgtca
      721 ggcagacctc cacggtccag cccatgggca cattaaacac atagcgggcg gcgatctgca
      781 ccaggaagac tgcgaacagc gccacaagca tggccgccgc cacgccttca gcggcgtggc
      841 gcagccattg ggtcagcggc ttcatcgctg aaacagcctg tctggcctat tttgccagtg
      901 cattgacggc atcgacaacg ccggcgggcc aggtcttcga gaagtccgat tcgagatagg
      961 ctttctgcac tgtggtgcgg aaggcctcga tatcaggctc gtagatcttg aggccctttg
     1021 ccttgaggcc ctcaaccagt tcagcttcct tcttgagctg gttctggcgg ccgaattcgc
     1081 aggcctcgtc cgccgccttc tgcagcttgg cctggttttc cggcgagagc gaatcccatg
     1141 ccttctttga gatggtcagg tagttgaggt cgacgaggtg cgaggtaagc acgatctgct
     1201 tggtcacttc ataatatttg gcgtccatca cggtgggcag cggattgtcc tggccatcca
     1261 ccgaacccgt ttgaagcgcg gtatagagtt ccgggaacgg caccggcacc gggttggcgc
     1321 ccagcgcctt gccaaggaat tgccaggcgt cggacggcgg catgcggagc ttgatgccgg
     1381 cgagatcggc cggtgtcctg atttccttgt cggtgcgcag gttgatctgg cggcggccga
     1441 gatacatgac cgagagcagc ttcacgccca gttccgtttc aatcttgtcc ttgaacggct
     1501 gcatcaacgg cgcattgaac acggccacct ggtgggcggc gtcgcggtgc agatagcccg
     1561 ccgagaaaac cgagaattcc ggaaagaagt tggcaatttc ctgggccgag gaaatcgaca
     1621 tttcgatatt gccgcgcgaa atggcttcaa gctcggtgcc ctgcttgaac agcgagccgc
     1681 cccagtaggg ctcgaaggtg gcaaaatcgg cgatggccgg gccaaatttc tcgatcaggg
     1741 caatggcgcg ctggtcggtg accgaattga cggacgacag gcgcagtttg agcttgtcct
     1801 gagccagg
//
LOCUS       DAORVN010000504         1806 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_73362_length_1806_cov_4.315248, whole genome shotgun sequence.
ACCESSION   DAORVN010000504 DAORVN010000000
VERSION     DAORVN010000504.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1806)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1806)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1806
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73362_length_1806_cov_4.315248"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            129..383
                     /gene="rpsP"
                     /locus_tag="PKV23_09585"
     CDS             129..383
                     /gene="rpsP"
                     /locus_tag="PKV23_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HQX85729.1"
                     /translation="MVVIRLARSGAKKRPFFNMVVADSRNRRDGRFIERIGYYNPVAA
                     ANENGLVINTERLAYWQGHGAQLSPTAARLVKQAAAPKAA"
     gene            398..895
                     /gene="rimM"
                     /locus_tag="PKV23_09590"
     CDS             398..895
                     /gene="rimM"
                     /locus_tag="PKV23_09590"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HQX85730.1"
                     /translation="MGRIVAPFGVHGWLRVHPFGDDPEAWRKMPRWWLSSDVEAPDER
                     WKAHDLEAIKIHGDGVVAKLAGIDDRDASEALGNLYFGAPRDALPQPAPDEYYWTDLI
                     GLAVVNMQDQALGRVKSLIETGANEVLVVVDGERERLLPFVEHVVKVVDVPGGTIRAD
                     WDSDW"
     gene            892..1635
                     /gene="trmD"
                     /locus_tag="PKV23_09595"
     CDS             892..1635
                     /gene="trmD"
                     /locus_tag="PKV23_09595"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010912321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HQX85731.1"
                     /translation="MSLRFDVITLFPEMFAALTASGITRRALDRGLWRIECWNPRQWT
                     QDAHRTVDDRPYGGGPGMVMMASPLEQAIAAAKVGAGGTAKVICLSPQGARITQQRVM
                     DLAASDGAILLCGRYEGIDERLIERCVDEELSLGDFVLSGGELAAMALMDACIRQLPG
                     ALNDEASAVEDSFAAGLLDCPHYTRPEVYEGCAVPDVLLSGNHERIRRWRMKQALGRT
                     WQRRPDLLEVRTLSSEEADLLAEFQREGK"
BASE COUNT          345 a          465 c          619 g          377 t
ORIGIN      
        1 ttcgtccggc aggttaggcg gcagaccggt tgggagagcc cgcgcggcca atagtcgaac
       61 gctgtgcctt gttttctccg gctttttgaa ataaaatcgc cggctttccc gctttccaag
      121 agaacaccat ggttgttatt cgtctcgccc gcagcggtgc caagaagcgc cctttcttca
      181 atatggtggt cgccgattcg cgcaaccgcc gcgatggccg cttcattgag cgcatcggtt
      241 attacaatcc agtcgccgcc gccaacgaaa acggcctggt gatcaatacg gaacgtctgg
      301 cctactggca ggggcatggc gcgcagttgt cgcccacggc cgcgcgcctg gtcaagcagg
      361 cagcggcgcc aaaggccgcc taagtcgcat gatcgtgttg gggcgcattg tcgccccgtt
      421 cggtgttcac ggctggttgc gggtccatcc cttcggcgat gatcccgagg cgtggcggaa
      481 gatgccgcga tggtggcttt cctcggacgt tgaggccccg gacgaacgct ggaaggcgca
      541 tgacctggaa gccatcaaga ttcacggcga tggcgtcgtg gcaaagcttg ccggcatcga
      601 tgatcgggat gcgtcggaag cgttgggcaa cttgtatttt ggcgccccgc gtgatgccct
      661 gccgcagccg gcgccggatg agtattactg gaccgacctg atcggtctgg cggttgtgaa
      721 tatgcaggac caagctctgg gccgggtcaa gtccttgatc gagaccggtg cgaatgaagt
      781 gctggtggta gtggacggcg agcgtgaaag gttgctgccg tttgtcgaac atgtagtaaa
      841 ggtggtggat gttccgggag gaacgattcg cgccgattgg gatagcgact ggtgagcttg
      901 cgcttcgatg tcatcacgct gttccccgag atgtttgcgg cgctcacggc gtcgggaatt
      961 acccggcgcg cgctggatcg ggggctgtgg cggatagagt gctggaatcc gcgccagtgg
     1021 acgcaggatg cgcatcggac ggtggatgac cggccttacg gcggtggccc cgggatggtg
     1081 atgatggcct cgccgctgga gcaggcgata gcggcggcaa aagtcggcgc tggcggaacg
     1141 gcgaaagtga tttgtctgtc gccgcaaggt gcaagaatta ctcagcaacg tgtaatggat
     1201 ctggctgcaa gcgatggggc gattctgctc tgtggcaggt acgaaggcat tgatgagcgc
     1261 ttgatcgagc gttgcgtgga tgaagagttg tcgctgggcg atttcgttct ttccggcggc
     1321 gaactggcgg cgatggcctt gatggatgcg tgcatcaggc aactgccggg tgcattgaat
     1381 gacgaggctt cggcagttga ggattcgttt gccgcgggct tgctggactg tccgcattac
     1441 acgcggccgg aagtgtatga aggatgtgcg gtgcccgacg tgcttttgtc gggaaaccac
     1501 gaaagaatcc ggcgctggcg catgaaacag gctctggggc ggacgtggca aaggcgccct
     1561 gatcttcttg aggtcaggac gttgagtagc gaagaagccg acctgctggc ggaattccag
     1621 cgggaaggta aatagaaacg gcacacatca ggacggtgaa tccgtcctgc tctgggcgca
     1681 aggttggcgg gtgtttccgc agccataact tgatggagta aagcaatgaa cctgatccag
     1741 caactcgata acgaagaaat tacccgcttg ggcaaaaaac tcccggtgtt ctcccccggt
     1801 gacacc
//
LOCUS       DAORVN010000505         1802 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_73702_length_1802_cov_2.880366, whole genome shotgun sequence.
ACCESSION   DAORVN010000505 DAORVN010000000
VERSION     DAORVN010000505.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1802
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73702_length_1802_cov_2.880366"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="PKV23_09600"
     CDS             <1..224
                     /locus_tag="PKV23_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013635210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQX85732.1"
                     /translation="SNIESITAVVIGGISLFGGRGSILGMIFGGLIVGVFSLGLRLLG
                     TDPQWTYFLIGVLIIGAVAIDQWIRKVSG"
     gene            230..1036
                     /locus_tag="PKV23_09605"
     CDS             230..1036
                     /locus_tag="PKV23_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020628808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQX85733.1"
                     /translation="MAKKAKADEPVPLLTARGLVKRYGRVTALDHADFDLYPGEILAV
                     IGDNGAGKSSLIKVICGAVLPDEGEIKLDGKTLNFKSPIEARDAGIETVYQNLALSPA
                     LSIADNMFLGREVRKPGLLGSLFRMLDRSTMERRSREKLTELGLMTIQNIGQAVETLS
                     GGQRQGVAVARAAAFGSKVIIMDEPTAALGVKESRRVLELILDVKRRGLPIVLISHNM
                     PHVFEVADRIHIHRLGRRLCVIDPKKYSMSDAVAFMTGAKTPPKELLAAS"
     gene            complement(1031..1744)
                     /locus_tag="PKV23_09610"
     CDS             complement(1031..1744)
                     /locus_tag="PKV23_09610"
                     /inference="COORDINATES: protein motif:HMM:NF025252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HQX85734.1"
                     /translation="MSEKPSFSATAMRQLLRRARTCSLSTLTRGDGTPYGSLANIATD
                     GAGRPLILISRLAWHTQNLEVDARSSVLAGELPAKGDALVGPRVTVLGRFERVEDANL
                     RRRYLARHPQAALYADFPDFSFWRLSPTAIHGVAGFGRIETLTPDEVFPVAAEMEALE
                     MSAIEHMNNDHAEAIHLYATKILKAQPGPWRVLAIDPDGAEIGLHEQSLRMEFHTPVF
                     TAAALRTKFAELAYEARTL"
BASE COUNT          346 a          567 c          519 g          370 t
ORIGIN      
        1 tctccaacat cgagtcaatc accgccgtgg tgatcggcgg catctcgctc ttcggcggac
       61 gtggctccat attgggcatg atcttcggcg gcctcatcgt cggcgttttc tccctgggtt
      121 tgcgccttct cggaaccgac ccgcaatgga cctacttcct gatcggcgtc ctcatcatcg
      181 gcgcggttgc aatcgaccaa tggatcagaa aggtttcggg ttaagcacca tggcaaagaa
      241 agcaaaagca gacgagcccg ttccgcttct caccgcccgc ggcctggtca agcgctatgg
      301 ccgcgtcacc gccctggacc acgcggactt cgatctttac cccggcgaaa tcctcgccgt
      361 gatcggcgac aacggggccg gaaaatcatc gctcatcaag gtgatttgcg gcgccgtgct
      421 gccggacgaa ggcgagatta agctcgacgg caagactctg aatttcaagt cgcccattga
      481 agcccgtgat gccgggatcg aaaccgttta tcagaatctc gccctgtcgc cggcgctctc
      541 cattgccgac aacatgttcc ttgggcgtga agtgcgcaag cccggcttgc tcggaagcct
      601 cttccgcatg cttgaccgtt cgacgatgga gcggcgctcc cgcgagaagc tcacggagct
      661 cggcctcatg accattcaga acattggcca ggcggttgaa accctgtccg gcggccagcg
      721 ccagggtgtg gccgtggccc gcgccgcggc ctttggctca aaggtgatca tcatggatga
      781 acctaccgct gccctgggcg tgaaggaatc ccgccgcgtc cttgaactga ttctcgacgt
      841 gaagcgccgc ggcctgccca tcgtgctgat ttcccacaac atgccccatg tcttcgaagt
      901 cgccgaccgc attcacattc accgcctggg ccggcgtctc tgcgtcatcg atcccaagaa
      961 atacagcatg tcggatgcgg tggccttcat gaccggcgcc aagaccccgc ccaaggaact
     1021 tctggcggcc tcatagggtc cgggcttcgt aggccagttc agcgaatttc gtgcgcagcg
     1081 ccgctgcggt gaaaaccggc gtatggaatt ccatccgcag gctctgctcg tgcagcccaa
     1141 tttccgcgcc atccggatca atcgccagaa cccgccaggg tccgggctgg gccttcaaaa
     1201 tcttcgtggc ataaagatgg atggcttccg catgatcatt gttcatgtgt tcgatcgccg
     1261 acatctccag ggcctccatt tccgcagcaa ccggaaacac ctcgtccggg gttaaggttt
     1321 caatgcggcc gaagccggca accccatgga tggccgtggg gctcaaccgc cagaaagaga
     1381 aatcgggaaa atccgcataa agtgcagcct gcggatggcg cgccagatag cgccgcctga
     1441 ggttggcgtc ttcaaccctt tcaaagcgcc ccagcacggt cacccgtggg cccaccaagg
     1501 cgtctccttt agccggaagc tcgcctgcca gtacgctgct ccgggcatcc acctcaaggt
     1561 tttgcgtgtg ccaggccagg cgtgaaatca ggataagagg cctgccggca ccgtcggtgg
     1621 cgatgtttgc cagggagcca tagggcgtgc cgtcgcctcg cgtcagggtg gaaagcgagc
     1681 aggttcgtgc ccggcgcagt agctggcgca tggcggttgc agaaaagctt ggtttttcag
     1741 acatttaatc aacattactc atttgtcatg caggccttct ttgcggtttg ccacaatttg
     1801 tt
//
LOCUS       DAORVN010000506         1801 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_73747_length_1801_cov_3.765178, whole genome shotgun sequence.
ACCESSION   DAORVN010000506 DAORVN010000000
VERSION     DAORVN010000506.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1801)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1801)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1801
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73747_length_1801_cov_3.765178"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(79..>1801)
                     /locus_tag="PKV23_09615"
     CDS             complement(79..>1801)
                     /locus_tag="PKV23_09615"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HQX85735.1"
                     /translation="TGNQLFIPRDSRSFAEAALGHLEGVFAHRVDSVFCGPVSAGCID
                     SEQETLGILAATGALSGLAGQGFDGLGEQVVAAALIKARAVRAHVFDAGSVCLYLGRV
                     HGKEALVYLDLPGQEIGPLVRQLLEILCANLRVSFENVVLFERLHFFAYFDPLTKLPN
                     RTRFLDRMDEGLSSFASEHWTVGIIDIVRFSELNDALGHRSGDLLLTEVTRRLKARLG
                     AEALVARVAGDAFGICWRPDEEGASAVLDVFSIPFFVRGHAVPMRARAGFAHAMALPG
                     GAVELLKRANLALSHAKLEGDQHWRFFSSEMEANTQSRVQLLNELRSALHLRKGLELH
                     YQPQVSPKSGHIVGCEALIRWIKPQGESVAPDVFIPLAEYTGLIVEIGDWVFSEACRQ
                     IVQWDSSGLPAFRVGINISAAQFREVDFVTKISAAIERSGVDPARLELEITESVAMED
                     PDSVARQLRELKALGLKVAVDDFGCGFSSLGSLNRLPFDRIKIDRAFVRELTPMTSQD
                     CIARLIVKLAKSLGLEVIAEGVETVEQARLLEEMGCKEMQGYLYGRPMSADALEGWIN
                     RDVVLKD"
BASE COUNT          430 a          586 c          459 g          326 t
ORIGIN      
        1 ggccgaatta taaagaacaa tcacaacgtg tcaacaagat taagaaaata aatcttactg
       61 gctctcctga cccccacgtc agtctttcag caccacatcc cggtttatcc aaccctccaa
      121 ggcatctgcg gacatgggcc gtccatagag gtaaccttgc atctccttgc accccatctc
      181 ttcaagcaag cgggcctgct ccaccgtctc gaccccttca gcgattacct ccaggcccaa
      241 gctcttggcg agcttgacga tcagtcgggc gatgcagtcc tgggatgtca tcggcgtcag
      301 ctcccgtaca aacgcacgat caattttgat ccgatcaaat ggcagacggt tgagactgcc
      361 gagcgaagag aagccacaac caaagtcatc caccgcgacc ttcaagccaa gcgctttcaa
      421 ctctcgcagc tgtctggcga cagagtcggg atcttccatg gcaaccgact cagtgatctc
      481 cagttcaagc cgtgccggat caacacctga gcgttcaatg gcagccgaga tcttcgtgac
      541 gaaatcgacc tcccggaact gggcagccga gatgttgatg ccgacccgaa aagcaggtaa
      601 gccgctactg tcccattgaa caatctgcct gcaagcttcc gagaaaaccc agtccccgat
      661 ctcgacgatc agccccgtgt actctgccaa gggtatgaag acatcaggcg ccacgctttc
      721 accctgaggc tttatccacc ggatgagcgc ctcgcacccg acgatatgcc cactcttcgg
      781 ggacacttgg ggctggtagt gaagctcaag gccctttcgc aaatgcaagg cggaacgtag
      841 ttcgttaagt agctgtaccc gactctgggt gttggcctcc atctccgagc tgaagaaccg
      901 ccagtgctgg tcaccctcca gcttggcgtg actgagggcg agattggccc gcttcaaaag
      961 ctccacggcc cctccgggaa gggccatggc atgggcgaat ccggcacggg cacgcatcgg
     1021 aacggcatgt ccccgaacga aaaagggtat cgagaacaca tccagaactg ccgacgcacc
     1081 ttcttcgtca ggacgccagc agattccaaa ggcgtccccg gcaacccgtg caacgagcgc
     1141 ttcagcaccc aggcgcgctt tcaacctgcg cgtgacttcc gtgagcagca gatcaccact
     1201 gcgatgcccc aatgcatcat tcagctccga gaatcgaacg atgtcgatga tccccaccgt
     1261 ccagtgctca ctggcaaagc tcgaaagccc ctcgtccatg cgatcaagaa atcgcgtccg
     1321 gttaggcaat ttcgtcaggg gatcaaagta tgcaaagaag tggaggcgct cgaagagcac
     1381 aacgttctcg aaactgacac gaagattggc gcacagtatc tcgagcagtt gccgcaccag
     1441 cggcccgatc tcctgcccgg ggagatccag atagaccagc gcttccttgc catgaacccg
     1501 ccccaagtag aggcacaccg accctgcatc gaatacatgc gcccgcactg cccgtgcctt
     1561 gatcagcgcg gcagccacca cctgctctcc aagcccgtca aagccctgcc cggcgaggcc
     1621 agacaaggcg cctgtcgcgg ccaggatgcc gagagtctcc tgctcactgt caatgcagcc
     1681 cgccgacaca ggaccgcaaa aaactgaatc cacccggtgg gcgaatacgc cctcaagatg
     1741 tcccagtgca gcctctgcaa acgagcggga gtctcgtgga atgaagagct gattccccgt
     1801 t
//
LOCUS       DAORVN010000507         1796 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_74099_length_1796_cov_3.367605, whole genome shotgun sequence.
ACCESSION   DAORVN010000507 DAORVN010000000
VERSION     DAORVN010000507.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74099_length_1796_cov_3.367605"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..644
                     /locus_tag="PKV23_09620"
     CDS             <1..644
                     /locus_tag="PKV23_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009465668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HQX85736.1"
                     /translation="IARAIVKDSDLIFLDEPLANLDYKLREELRDELPKLFSGRNAIV
                     FYATTEPHEALLFGNQTLTMHEGRITQFGPTAEIYRKPNSLVSARVFSDPPINIAKVT
                     KKGRSLQFAHGDVTWPVAPSAARLPDGDYSIGLQPHYVLPQKASETSGALSGRVLVTE
                     ISGSESVVHIDVGGQTWVSQSHGVHPHQVGEMATFHADVSHALFFDSAGKRVS"
     gene            644..1717
                     /locus_tag="PKV23_09625"
     CDS             644..1717
                     /locus_tag="PKV23_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018246651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQX85737.1"
                     /translation="MAKITIDNLRHSYMPEPKRDEDFALKRVHLDWADGGAYALLGPS
                     GCGKTTLLNLISGLLHPTEGRILFDGRDVSNETPEHRNIAQVFQFPVIYDTMTVYDNL
                     AFPLRNRKVDEAEIKKRVTAIAEMLELTATLSKRASGLTADGKQKISLGRGLVRSDVN
                     VIMFDEPLTVIDPHLKWQLRSKLKELQHRIRRTMIYVTHDQTEALTFADQVVVMNNGE
                     VVQTGTPIELFEKPKHIFVGHFIGSPGMNVMPCQVRGGKAFVEGHAVAADNAASSKGS
                     GRLELGIRPEFVTFADKGLPVDVVKVSDIGRYRVVDTRLGENSVKLIVPEGGAIPEGK
                     AHLAFDKAHTQIYSDGWLAGEAR"
BASE COUNT          359 a          574 c          517 g          346 t
ORIGIN      
        1 ccatcgcccg cgccatcgtg aaggacagcg atctcatttt cctcgacgaa cccctggcca
       61 accttgatta caagctgcgc gaggaactgc gcgacgaatt gccgaaactt ttctccggcc
      121 gcaatgccat cgtgttttat gcaactaccg agccccacga ggcgctgctg ttcggcaacc
      181 agacgctgac catgcatgaa ggccgcatca cccagtttgg cccgacggcc gagatttacc
      241 gcaagcccaa ttcgcttgtc tcggcgcggg tgttttccga tccgccgatc aatatcgcca
      301 aagtcaccaa gaagggcagg agcctgcaat ttgcccatgg cgacgtgacc tggcccgtgg
      361 cccccagcgc cgccaggctc ccggacggcg actacagcat cggcctgcag ccccactacg
      421 ttctgccgca gaaggcctcg gaaaccagcg gcgcgctttc aggccgggtg ctggtgaccg
      481 aaatttcggg gtcggagagc gtggtgcata tagatgtggg cggccagacc tgggtgtcgc
      541 agtcccatgg cgtgcatccc caccaggtgg gcgaaatggc caccttccat gccgatgtct
      601 cccatgccct gttttttgat tctgccggaa agcgggtgtc ctgatggcca agatcacgat
      661 cgacaatctg cgccattcct acatgccgga acccaagcgc gacgaagatt ttgccctcaa
      721 gcgcgtgcat ctcgattggg ccgacggcgg cgcctacgcg cttctgggtc cctcgggttg
      781 cggcaagacc accttgctca atctgatctc cggcctgctt cacccgacgg aaggccgcat
      841 cctgtttgac ggccgcgacg tcagcaatga aacacccgaa caccgcaaca tcgcccaggt
      901 gttccagttc ccggtcatct acgacaccat gacggtctac gacaatctcg cctttcccct
      961 gcgcaaccgg aaggtggatg aagccgagat caaaaagcgg gtgactgcca tcgccgaaat
     1021 gctggagctg acggcaacgc tttcgaaacg cgcttctggc ctcaccgctg acggcaagca
     1081 gaagatttcc ctgggccgcg gccttgtccg ctccgatgtt aacgtcatca tgttcgatga
     1141 accgctcacc gtcatcgatc cccatctcaa atggcagctg cgatccaagc tcaaggagct
     1201 gcagcaccgc atcaggcgga ccatgatcta cgtgacccat gaccagaccg aggcgctgac
     1261 ctttgccgac caggtcgtgg tgatgaacaa tggtgaggtg gtgcagaccg gaacccctat
     1321 cgagcttttc gagaagccca agcatatttt cgtgggccat ttcatcggct cgccgggcat
     1381 gaatgtcatg ccctgccagg tcaggggcgg caaagccttc gtggaaggcc atgcggttgc
     1441 cgccgacaac gccgcgtctt ccaaggggtc aggccgcctg gaactgggca tccggccgga
     1501 atttgtgacc ttcgccgaca agggcttgcc cgtggatgtc gtgaaggtgt ccgacattgg
     1561 ccgctaccgc gtggtcgaca ccaggcttgg cgagaattcc gtcaagctga tcgtgccgga
     1621 aggtggcgcc atccccgagg gcaaggcgca tctggcattc gacaaggccc atacgcagat
     1681 ctacagcgat ggctggctgg cgggagaagc ccgatgaaca agaccttcaa caacaaggcc
     1741 tggtggatgg tgctccccgt cttcctggtg gttgccttca acgcccttgt gccgct
//
LOCUS       DAORVN010000508         1794 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_74232_length_1794_cov_4.150661, whole genome shotgun sequence.
ACCESSION   DAORVN010000508 DAORVN010000000
VERSION     DAORVN010000508.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1794
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74232_length_1794_cov_4.150661"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            85..1602
                     /locus_tag="PKV23_09630"
     CDS             85..1602
                     /locus_tag="PKV23_09630"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HQX85738.1"
                     /translation="MKRPVPTAAAALATKLRAGPDGFIRLQIDERDWIGLAMGCAQGL
                     QDLLALWVDDDAAHMGLGVPALKLRIIVSVPLKRGSFPSVAKNHPAACRLERSIRDLH
                     GVEPKGLPDQRNWMDHGKWPSPPPSLKKDAARRDAAYAFLATEGEGLHQIAVGPVHAG
                     IIEPGHFRFSVNGEAVVRLEERFGYTHKGIAALCKGADIATAARIAARISGDSTVAYS
                     YAYALAIEAALKWRPPPRAILLRGIMAEIERLSHHVNDVGAICNDASVVVLHAHCTLL
                     REDILRTANQCFGHRMMMDRIVPGGVAADLSAAGAEAIQALLARCATNFEAVVRVYDE
                     APSLQDRTVSTGNVSAELARRFGAGGHVGRASGRAFDTRLNFPYGPYEALDFAMALHA
                     GGDVDARVWVRIEEVRSSMALIRQMLKLLVPGPLMSVMPRARASEGAAMVEGFRGDIF
                     VTVRLDDRARLVNFYARDPSCFQWPLLEAAIEGNIVADFPLCNKSFNCSYSGHDM"
BASE COUNT          331 a          545 c          541 g          377 t
ORIGIN      
        1 tcatcttggg ttggttctta tcgctggcat ctacttgccg ccgacgctgg taggctggtt
       61 cgaacgcgct gcggagctgc tcaagtgaag cggcccgtac caaccgccgc tgccgcgctg
      121 gccaccaaac ttagggcggg gccagacggc tttatccgtt tgcagattga tgagcgggac
      181 tggatcggcc ttgccatggg ctgtgcccag ggcttgcagg atctcctggc actctgggta
      241 gacgatgatg ccgcccacat ggggctcggc gttcctgcac tcaagttgcg aatcattgtg
      301 tccgtgccgc tgaaacgcgg cagtttccca tcggtagcga aaaatcaccc agccgcctgc
      361 cgcctcgaac gcagcattcg cgatctccat ggcgttgaac ccaaaggcct gcccgatcaa
      421 cgcaactgga tggatcacgg caaatggcca tccccaccgc caagcctgaa aaaggacgca
      481 gcccgtcgag acgcagctta tgctttcctg gctacggaag gcgaagggct gcatcagatt
      541 gctgttgggc cggtgcacgc cgggatcatc gaacccggac atttccgctt ctctgttaac
      601 ggtgaagcgg tcgttcggct cgaagagcgg ttcggttaca cccacaaggg aattgcagcg
      661 ttgtgcaagg gcgccgatat cgccacggca gcacgcatcg cggcgcgaat ttcgggcgat
      721 agcactgtag cctattccta tgcctatgcg cttgccatcg aagccgccct caagtggcgg
      781 cctccgccgc gtgccatctt gctgcgcggc atcatggcgg agattgaacg cctgtcgcac
      841 catgtcaatg atgtgggcgc tatctgcaac gatgccagcg tcgtggtgct ccatgcccac
      901 tgcacattgc tgcgcgagga tatcttgcgg actgccaacc agtgttttgg ccaccgcatg
      961 atgatggatc gcatagtgcc gggtggtgtg gccgctgatc tttcggctgc aggggctgag
     1021 gcgatacagg cgctgcttgc gcgctgcgcc acgaatttcg aagcggtcgt gagagtctat
     1081 gacgaagcgc catcattgca ggaccgcacg gtttcgacag gcaacgtcag cgcagaactt
     1141 gcccggcgct ttggagccgg cggccatgtc ggtcgtgcct cgggccgggc tttcgacacg
     1201 cgcctgaatt ttccctatgg accttacgaa gcactggact tcgccatggc acttcacgcg
     1261 ggaggcgatg tcgacgcccg ggtatgggtc cgcatcgaag aagtccgctc aagcatggcg
     1321 cttatccggc aaatgctgaa gctgctggtt cccggccctc ttatgtctgt catgccccga
     1381 gcgcgcgcca gcgaaggcgc tgcaatggtt gagggtttcc gcggtgatat atttgtcact
     1441 gttcggctgg acgacagggc aaggctggtg aatttctatg cccgcgaccc atcctgcttc
     1501 cagtggcccc tgcttgaagc cgcaattgaa ggcaacatcg ttgccgactt cccgctctgc
     1561 aacaaatcat tcaactgttc ctattccgga cacgacatgt aggacaccat gcgctcactt
     1621 ctcttcaaat cgctacttga gggctccgcc gtaatcgcgc cgccgcaacc cggcagtgca
     1681 gctgtgactg aacttgctga agcgctgggc cagcaggcac acgcccgcct cggccggtcc
     1741 ttggcgatcc gcgaggtgga tgccggatct tgcaatggct gggaactgca aatg
//
LOCUS       DAORVN010000509         1783 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_75055_length_1783_cov_4.277199, whole genome shotgun sequence.
ACCESSION   DAORVN010000509 DAORVN010000000
VERSION     DAORVN010000509.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1783
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75055_length_1783_cov_4.277199"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="PKV23_09635"
     CDS             complement(<1..252)
                     /locus_tag="PKV23_09635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85739.1"
                     /translation="MRRLIPLNPSVRCIGVASSLGAPFSGCEMGPSSLKKAGLAAVIR
                     HNGMAVEWQEMLLPDTSTAAMSVLLGGLADATAAAIVAGQ"
     gene            complement(425..1435)
                     /locus_tag="PKV23_09640"
     CDS             complement(425..1435)
                     /locus_tag="PKV23_09640"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HQX85740.1"
                     /translation="MKRSAWLLAGMLSIGTCMADDKVLNLYSARHYQADEQLYSGFTE
                     KTGIRINRIEGKSNELIERIRNEGANSPADVLLTADAASLAVADSWNIFAPVHSEFLE
                     KRIPAHLRTDKWFSFAVRARMIIFNKRKLKAEDVATYQSLADPKLKGLICSIPGGHIY
                     NLTLVASLVVHDGEAKTEEWARGVAGNLARSPRGGDTDQIKSVASGECAVAVSNSYYL
                     ARLMGSEKVEERNMTKRLGIVWPNQGSSGTHINVSGGGLVKTAKNRESAVRFLEYLAS
                     DEAQRYFAEGNNEWPAVPGVKVDNEELQELGTFKADQTPLTTIARQLPVAQKLLDRAG
                     YR"
     gene            complement(1462..>1783)
                     /locus_tag="PKV23_09645"
     CDS             complement(1462..>1783)
                     /locus_tag="PKV23_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011086682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HQX85741.1"
                     /translation="AEPDEYEGGELEIEGPFGIQAAKLEAGDMVLYPSSSLHRVTPVS
                     AGARVASFFWIQSMVREDSARTLLFDLDQSIQAMTPLVAPDDPRLLKLTGVYHNLLRL
                     WAVT"
BASE COUNT          335 a          554 c          515 g          379 t
ORIGIN      
        1 ctgaccggca acaatggctg ctgccgtggc atcggccaag ccgccgagca agaccgacat
       61 tgctgcggtc gacgtatcag ggagcagcat ttcctgccat tcgacggcca tgccgttatg
      121 ccgtatcact gccgccagac ctgccttctt cagagaagac ggccccattt cgcaaccgga
      181 aaacggcgcc ccgagagaac ttgccacccc aatgcaacgc actgacgggt tgagggggat
      241 gagcctgcgc atgaagaaac tcctttccgc atcctggatc ctgcggcttc gtcaatttga
      301 ccgatatgaa cctggcaagt tcactcaatc taaaattttg agagacttcg gaccaaataa
      361 aagttgctgc gggtctgtcc gggcaaggca aggcttcatc cgcgttcaaa cgcgttttcc
      421 aggttcagcg gtaacctgcc cggtcgagca gtttctgggc gacgggcagt tgccgggcaa
      481 tggttgtcag cggcgtctgg tctgccttga atgtgcccaa ctcctgcagt tcctcgttat
      541 cgaccttgac gcccggtacg gccggccact cgttgttacc ctcggcaaaa tagcgctggg
      601 cctcgtcgct ggccaggtat tccaggaagc ggacggcaga ctcccggttc tttgccgtct
      661 tgaccagccc gccaccggaa acgttgatat gcgtgccgct gctgccctgg ttcggccaga
      721 caattcccag gcgcttggtc atgttgcgtt cctcgacctt ttcggagccc atcaggcggg
      781 ccaggtaata actgttggac accgccacgg cgcattcacc actggccacc gacttgatct
      841 ggtcggtatc gcctccccgt gggctgcgcg ccaggttgcc ggcgacgccg cgcgcccatt
      901 cctcggtttt tgcctcgccg tcatgcacca ccagtgaggc gaccagagtc aggttgtaga
      961 tatgcccacc gggaatgctg cagatcaagc ccttcaactt cggatcagcc agcgactggt
     1021 aggtcgccac gtcttcggcc ttgagcttgc gcttgttgaa gatgatcatc cgggcgcgaa
     1081 ccgcgaagga aaaccatttg tccgtccgca agtgggcagg aattcgcttc tccaggaatt
     1141 ccgagtggac cggcgcaaag atattccagg agtctgccac ggcgagactg gctgcatcgg
     1201 ccgtcaacaa gacatcggcc ggactgttcg ccccctcgtt gcggatacgc tcaatcagtt
     1261 cgtttgactt tccctcgatg cggttgatcc ggatgccggt cttctccgtg aagccgctgt
     1321 acagttgttc atctgcctgg taatggcggg ccgaatagag gttgagtacc ttgtcgtccg
     1381 ccatgcaggt gccgatggag agcatcccgg ccagcagcca cgcgcttcgt ttcattgact
     1441 tgttctccgt gttgccttgc atcaggtcac cgcccacagg cggagcaggt tgtgataaac
     1501 gccggtcagc ttgagcagac gcggatcatc cggcgcaacc agcggagtca tcgcctgaat
     1561 ggattgatcg agatcgaaca gcaaggtgcg ggcgctgtct tcacggacca tgctctggat
     1621 ccagaagaac gaagcgacgc gggcgcccgc gctgaccggc gtcacccgat gcaggctgct
     1681 tgacggatag agcaccatgt caccggcttc cagcttggcg gcctggatgc cgaacggccc
     1741 ttcgatttcc agttcgccac cctcgtactc gtccggctcg gcc
//
LOCUS       DAORVN010000510         1783 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_75108_length_1783_cov_2.350116, whole genome shotgun sequence.
ACCESSION   DAORVN010000510 DAORVN010000000
VERSION     DAORVN010000510.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1783
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75108_length_1783_cov_2.350116"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1325
                     /locus_tag="PKV23_09650"
     CDS             <1..1325
                     /locus_tag="PKV23_09650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85742.1"
                     /translation="LSRPEGEKPADAYDRATARNIRAIWNRLRPPFTALLLAVSLFCI
                     VFFSSLNFSTARLPGSDGVSTAARGLQFTVEGEAAAARLVEDLQRIPEVGTVRWMGNF
                     QPQQVELKRKILQGLAGAIPGVSEGGAVGPYDLLEHMRGMEAGLRVISDGAGAAGGLR
                     ASAHELRRSLAVLANSGRAPESTTAELERLLFSGFGDLSKNADGLAVLTEPKLADLDP
                     NLRALYVTEGDRWRIEALPRRLISAKAFIDATRVVDAIALGPLVAEQAQLRTLASASR
                     TALAFGFVFTLLMTLAYLRNILDWLIVVASSFLLLPLYAVFIVTTATAVSPATLPALV
                     MTSLYGVTTALLLVAARRRQGAGGLAILLPVAAIVALILPMRLLNLPEFESFTGTLTM
                     LLVVATAFNLTVVPQLCAWTEGWRSTDPAPDDAKRPARPREELGEDIF"
     gene            1543..>1783
                     /locus_tag="PKV23_09655"
     CDS             1543..>1783
                     /locus_tag="PKV23_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006330880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HQX85743.1"
                     /translation="MNFKSWLVGTTMLVMGTTGAFAEVVFNRGNSADPESLDPHKTST
                     VYEAHILRDLFMGLTIHNAKAEVEPGAAESWTISPD"
BASE COUNT          310 a          565 c          536 g          372 t
ORIGIN      
        1 ttctttcaag gccggaagga gaaaagcccg cggatgccta tgatcgcgcc acagcccgca
       61 acattcgggc gatctggaac aggctgaggc cgcccttcac cgcgctgctc ctggccgtat
      121 cgctcttctg cattgtgttc ttttcgtcgc tgaatttcag caccgcacgc cttcccgggt
      181 cagatggtgt ctccaccgcc gcacgggggc tgcaattcac cgttgaaggg gaagccgccg
      241 ccgccaggct tgtggaagac ctgcagcgga ttccggaagt gggtacggtt cgctggatgg
      301 ggaactttca gccgcagcag gtcgaactga agcgcaagat attgcagggt cttgccggag
      361 caattccggg ggtgagtgag ggcggcgcgg tcggacccta tgatctgctt gagcatatgc
      421 gtggaatgga agcgggcttg cgcgtcattt cagatggcgc gggcgctgct ggcggcctgc
      481 gcgccagcgc ccacgagctt cgccgttcac ttgccgtgct tgccaattcc ggcagggcgc
      541 ctgaatcaac cacagcggaa ctcgagcggc tgctcttttc cggcttcggc gacctgtcaa
      601 aaaacgccga tggcctcgcc gtcctgaccg aacccaagct cgctgatctc gacccgaacc
      661 tgcgcgccct ttacgtgacc gaaggcgaca ggtggcggat tgaagccctg cccaggcgat
      721 tgatctcggc caaggctttc atcgatgcaa cgcgggtggt ggatgccata gcccttggcc
      781 ccctcgtggc cgaacaggcg caattgcgca cccttgcatc ggcctcccgg acggcgctcg
      841 catttggctt cgttttcacc ctgctcatga ccctcgccta tctgcgcaac attctggact
      901 ggctgattgt ggtggcctct tcgtttctgc tcttgccgct ctatgcggtg tttatcgtga
      961 caacggcaac ggcggtcagc ccggccacgc tgcccgccct ggtcatgacc tcgctgtatg
     1021 gtgtcacgac cgcgctgctc ctggtggccg cgcggcgccg gcagggcgca ggcgggcttg
     1081 ccattcttct cccggtggcg gcgattgtcg cacttatcct gccaatgagg ctgctgaacc
     1141 tgcctgagtt cgagtccttt accggcacgc tcacgatgct tttggtggtt gccacggcct
     1201 ttaacctgac cgtcgttccg cagctctgcg catggaccga gggctggcgc tccacggatc
     1261 cggccccgga cgatgcgaaa cgccccgccc gcccgcgcga agagctgggc gaagatatct
     1321 tttgacgtct gcacaggcat gcgcaaacgc gatttgacag ccgcaatcgg cgtttgggcg
     1381 cctgatccgg ctgcccgccc ctgcctttcg cgccgcgctg cgacgaaagt caagggcgac
     1441 aaccgtaaaa tctggtgcta ggttttcagg catgagccaa aagcttttgg cgctcgccta
     1501 cgtaaattag aatcgtaaat gagggagtat ccattcatga acatgaactt caaatcctgg
     1561 cttgtcggca ccaccatgct ggtcatgggc accaccggcg cctttgcgga ggtggtcttc
     1621 aaccgcggca actcggccga tccggaatca ctcgatccgc acaagacctc caccgtctat
     1681 gaagcccata tcctgcgtga cctgttcatg gggctgacga ttcacaacgc caaggcggaa
     1741 gttgagcccg gtgctgccga aagctggaca atctcgccgg atg
//
LOCUS       DAORVN010000511         1782 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_75192_length_1782_cov_2.035900, whole genome shotgun sequence.
ACCESSION   DAORVN010000511 DAORVN010000000
VERSION     DAORVN010000511.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1782)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1782)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1782
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75192_length_1782_cov_2.035900"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..454)
                     /locus_tag="PKV23_09660"
     CDS             complement(<1..454)
                     /locus_tag="PKV23_09660"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HQX85744.1"
                     /translation="MSNVEAAVEPRNEYVDFWNTILVPKFVRWRHIIVDGLTLHSAKI
                     FPSLEVQEGDKVVDAGCGFGDTAIQLARLVGPTGSVLAVDCCDGFLAYGRDDARAAGV
                     SNVTFLEADVQSYPFKPEHDFCFSRFGTQFFESPVAGLRNMRKTLKPGG"
     gene            complement(475..909)
                     /locus_tag="PKV23_09665"
     CDS             complement(475..909)
                     /locus_tag="PKV23_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007695318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HQX85745.1"
                     /translation="MTNANTNKGKCFCGSVEFAVTGEPAAMGYCHCSSCREWSAGPVN
                     AFTLWPPEALKITKGAENIGVHHRTDRSLRKWCKTCGGHLFTDHPHWNLVDVYAAVIP
                     SVEFKPGVHVHYQETVLPVKDGLPKQKDVPKEMGGSGVLLAE"
     gene            complement(959..1159)
                     /locus_tag="PKV23_09670"
     CDS             complement(959..1159)
                     /locus_tag="PKV23_09670"
                     /inference="COORDINATES: protein motif:HMM:NF018294.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HQX85746.1"
                     /translation="MRSKTTAGFLQANSSFFRIAVTKITAAIERRRIRTALGRLDNYM
                     LKDLGITRSDIERIANRTYPPR"
     gene            complement(1182..1682)
                     /locus_tag="PKV23_09675"
     CDS             complement(1182..1682)
                     /locus_tag="PKV23_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012757845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85747.1"
                     /translation="MKTYRLPVGLALLSVLAAASALADSANPLVDKVRAANDRFKDVA
                     VAVAEGYSPIPCASGLQGGAMGVHYVNGALLKDGIVDIAKPEAVMYEPKADGKMDLVA
                     VEYIAFKGPAALDDHLFNFVTEPNRYGLDKFYELHVWAWRPNPTGAFADNNPNVSCDA
                     MHGAAH"
BASE COUNT          362 a          530 c          513 g          377 t
ORIGIN      
        1 cgccgcccgg cttgagcgtc ttgcgcatgt tgcgcaagcc ggcgacgggg ctttcaaaga
       61 attgcgtgcc aaagcgcgag aagcagaaat catgctccgg cttaaaggga taggactgca
      121 cgtcggcttc cagaaatgtc acgttggaaa cgccggcggc cctggcatcg tcgcgcccgt
      181 aggcgaggaa cccgtcgcag cagtcaaccg ccaggaccga tccggtggga cccaccagcc
      241 gggcaagctg gatcgcggtg tcgccgaaac cgcagcccgc atcaacgacc ttgtcgcctt
      301 cttgcacttc gagcgaaggg aagatcttgg cgctatgcag ggtcaggcca tcgacgatga
      361 tgtggcgcca gcggacgaat tttggaacaa gaatggtgtt ccagaaatcg acatactcgt
      421 tcctgggttc aactgcggct tccacattgc tcatcttcat ctcctcaatt cagcctattc
      481 ggccaacagg acgccggaac cgcccatttc tttcggcacg tccttctgct tgggcaggcc
      541 gtctttcaca ggtagcacgg tttcctggta atggacatgc accccgggct tgaactccac
      601 cgacggaatg accgcggcat agacatcaac caggttccag tgcggatgat cggtgaagag
      661 atgcccgccg caggtcttgc accacttccg caggctcctg tcggtccggt gatggacccc
      721 gatgttttcc gcgcccttgg tgatcttgag cgcctcgggc ggccatagcg taaaggcatt
      781 gacgggcccg gccgaccact cccggcagga actgcagtgg caatagccca tcgcggcggg
      841 ttccccggta acggcgaact caacggaacc gcaaaagcac ttccccttgt tggtatttgc
      901 gtttgtcatc taggtcttcc tttcattcag agatgggttt gattggaggg agcaatcact
      961 aacgcggtgg gtaggtccgg ttggcaatcc tctcgatgtc ggaccgggtg atgcccagat
     1021 ccttcaacat gtaattgtcc agtctgccga gcgccgtgcg gatccggcgc cgttcaatcg
     1081 ctgcggtgat tttagtcacc gcaatgcgaa agaaactgga attggcctgc aggaatcccg
     1141 cagtggtctt gctccgcatc acttgctcca aagtatttct gctaatgggc cgcgccgtgc
     1201 atggcgtcgc agctcacatt gggattgttg tcggcaaagg cgccggttgg attgggccgc
     1261 caggcccaga catgcagctc gtagaacttg tcgagaccat agcggttcgg ctcggtcacg
     1321 aagttgaaga ggtggtcatc cagtgccgcc ggccccttga aggcaatata ttcaacggcc
     1381 acaagatcca tcttcccgtc ggctttcggc tcatacatca ccgcctcggg ttttgcgata
     1441 tcgacgatcc catctttgag aagcgcaccg tttacatagt gcacacccat ggcgccgccc
     1501 tgcaggccgc tggcgcaggg aatgggtgag tagccttcgg ccaccgccac cgccacatcc
     1561 ttgaagcggt cattggcggc gcggaccttg tcaaccagcg ggttggcgct gtcggccaat
     1621 gccgatgcag ccgcaagcac ggatagaagg gcaaggccca caggcaaacg atatgtcttc
     1681 atgtcagtct ccagtttgtt ttgctttttg caatgatcag gcatggccag aaccagccat
     1741 cagtcggcag caccctagaa aaacaccgtt attgctccgt ca
//
LOCUS       DAORVN010000512         1776 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_75629_length_1776_cov_2.704242, whole genome shotgun sequence.
ACCESSION   DAORVN010000512 DAORVN010000000
VERSION     DAORVN010000512.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1776)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1776)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1776
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75629_length_1776_cov_2.704242"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(23..802)
                     /locus_tag="PKV23_09680"
     CDS             complement(23..802)
                     /locus_tag="PKV23_09680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85748.1"
                     /translation="MKIEFQTANTLAAHAVCVAAGTRDGLRTAQFYVRDMSRADSGNG
                     RRNVAFLSALVARGARLADRFMTWSANLAIALFLPSWRKLPSPFAPGMMNEVADAIRK
                     NSLVHNPLFNAYFFRAAIHIIKRYSEPPYLLLEHRVDAARRQLATSEESALPVTETGF
                     LARTLLALVETAPVARVGKALGPVRLFEDSEPNIAVFAIACVTLLFAEEGKPIAIQDE
                     DEFFTIVGALILPRLKAISLLIASRDVAGLSRELAAIKAMY"
     gene            complement(811..1641)
                     /locus_tag="PKV23_09685"
     CDS             complement(811..1641)
                     /locus_tag="PKV23_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014762656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HQX85749.1"
                     /translation="MAAKPEKGIWLRKLVLAIWGLLCFLPILFFATIALRPRNEIIAP
                     QPIYFPTLTIETWAKILEEWPLLTYFKNSVMAIGGSVFICLLLGIPAAYALARYKFRG
                     REDIGFYILSTKMMAPAAVAIPIFVLFGQFRILDTVWALMLVYAAMNLSIVVWIIRSY
                     MLDIPPEIEDAARTDGASDLRILWSIIIPMCLPGIVTAAVIAAIFAMNEYLFTLIIAS
                     RNAYPLSVALANFSGGSDGIIYNAIALVSFMAFFPILLLVILIQNHLSRGLTMGAVKG
                     "
BASE COUNT          418 a          488 c          555 g          315 t
ORIGIN      
        1 gacgaaggtt agagcctagg gctcagtaca tcgccttgat agcggccagt tccctggaaa
       61 gcccagccac atcacgactt gcgatgagca gtgagatggc cttcagcctt ggcagtataa
      121 gggccccgac aatggtgaag aattcatcct cgtcctggat ggcaatgggc ttgccttcct
      181 cggcgaacag aagcgtgacg caggcaattg cgaacaccgc gatgttgggt tcgctgtcct
      241 caaacaggcg caccggcccc agggcctttc cgacgcgcgc cactggcgcg gtttcaacca
      301 aggccaaaag tgtgcgcgcc agaaaacccg tttcggtcac gggcagggca ctttcttcgc
      361 tggtggccag ttgccgccgc gccgcgtcaa cccggtgctc aagcagcaga tagggcggct
      421 cagaatagcg cttgatgatg tggattgcgg cgcggaagaa ataggcgttg aacagcggat
      481 tgtgaaccag gctgttcttc cggatggcat cagccacttc attcatcatg cccggcgcga
      541 atggcgacgg cagcttgcgc cagctcggaa ggaaaagcgc aattgccaga tttgcgctcc
      601 aggtcatgaa gcggtccgcc aggcgcgcgc cgcgggcaac aagcgccgac aggaaggcaa
      661 cattccgcct gccattgccg ctgtcggcgc gcgacatgtc acgcacatag aactgggctg
      721 tccgcaagcc atcgcgcgtg ccggcggcaa cgcatacggc gtgggcggcc agggtattgg
      781 cggtctggaa ttcaatcttc atcggccaat tcaaccctta accgcgccca tcgtcaagcc
      841 gcgcgacagg tgattctgaa tcaggattac cagaagcaga atcgggaaga aagccatgaa
      901 ggaaaccagc gcaatggcgt tgtagatgat gccgtccgac ccgccggaaa aattggcaag
      961 cgcaacggac aggggatagg cattcctgga ggcaatgatg agcgtgaaga ggtattcgtt
     1021 catggcaaaa atggcggcga tgacggcagc ggtcacaatg cccggcaggc acatgggaat
     1081 gatgatagac cacaggatgc ggaggtcaga ggcgccatcg gtgcgtgccg cgtcctcaat
     1141 ttccggcggg atatcgagca tgtaggagcg gatgatccac accacgatgg acaggttcat
     1201 cgccgcgtaa accagcatga gggcccagac cgtatcgaga atgcggaatt gcccgaacag
     1261 gacaaagatt ggaatggcaa ccgcggccgg cgccatcatc ttggtcgaga ggatgtagaa
     1321 cccgatgtct tcgcggcccc tgaatttgta gcgcgccagc gcgtaggctg cggggattcc
     1381 aagcagcagg cagatgaata cgctgccgcc gatcgccatc accgagttct tgaaataggt
     1441 gaggaggggc cattcctcca aaatcttggc ccatgtctcg attgtcagcg tgggaaaata
     1501 gatgggctga ggagcaatga tctcgttgcg cggccgtaac gcgattgtcg caaagaacag
     1561 gatgggaaga aagcacagga gcccccagat cgcgaggacc agcttgcgga gccagatgcc
     1621 tttctcaggt ttcgccgcca tcactcaact cccgacttga agaagcgccg caccagcgtc
     1681 tgcgccagga tgatcgtggc gatcagcagc aggacagagg ctgctgaggc atagccttgt
     1741 tcaaagctct tgaaggctgt tcggtaaatg aagtag
//
LOCUS       DAORVN010000513         1775 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_75686_length_1775_cov_3.152907, whole genome shotgun sequence.
ACCESSION   DAORVN010000513 DAORVN010000000
VERSION     DAORVN010000513.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1775
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75686_length_1775_cov_3.152907"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..998)
                     /gene="eno"
                     /locus_tag="PKV23_09690"
     CDS             complement(<1..998)
                     /gene="eno"
                     /locus_tag="PKV23_09690"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012335557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HQX85750.1"
                     /translation="MTAILDITAREILDSRGNPTVEVDVILEDGSFGRAAVPSGASTG
                     AHEAMELRDGDKKRYSGKGVLNAVAAVNGEIFETLIDMDAEDQIAVDRAMIALDGTPN
                     KSRLGANAVLGVSLATARAAAEACGQPLYRYVGGTVARTLPVPMMNIINGGAHADNPI
                     DFQEFMILPVGAPSFKEALRMGAEVFHTLKASLKKAGHNTNVGDEGGFAPNLPSAEAA
                     LEFAFKAIEQAGYKPGKEIYLGLDCAATEFFKNGVYDYEGEGKKRSIAEQAKYLAKLA
                     KDFPIISIEDGMSEDDWDGWKQITDLIGKTHQLVGDDLFVTNTARLQRGITEHIANS"
     gene            complement(1065..1766)
                     /locus_tag="PKV23_09695"
     CDS             complement(1065..1766)
                     /locus_tag="PKV23_09695"
                     /inference="COORDINATES: protein motif:HMM:NF014068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HQX85751.1"
                     /translation="MHAKGAILASVCSGSMLLGQTGLLDGEDATSHWGYCDTMSRNFP
                     KIRMRRERILVPAGIGHRLITAGGATSWYDLLLYLIGRFAGPEEARRVAKVFLLQSHG
                     QGQLPFAGLTARRQHSDEMVKSAQLWIADNYMKANPVAAMASNCGLTERSFHTRFKRA
                     TGMSPMDYVPTVRIEEAKQLLETSEMPLDEVAAEVGYAEPASFRRLFGRMVGVTPSTY
                     RRQHLPVNLSIREAN"
BASE COUNT          345 a          494 c          541 g          395 t
ORIGIN      
        1 gaattggcaa tatgttcggt gattccgcgc tgcaggcgcg cggtgttggt cacaaacagg
       61 tcgtcgccta ccagctggtg ggtcttgccg atgagatctg tgatttgctt ccagccgtcc
      121 caatcatctt ccgacatgcc gtcttcaatg gaaatgatcg gaaagtcctt ggcaagcttg
      181 gccagatatt tcgcctgctc ggcaatggag cgcttcttgc cttcgccctc gtaatcatag
      241 acgccgttct tgaaaaactc ggtggcggca cagtcgaggc ccaggtaaat ttccttgccc
      301 ggcttgtagc ctgcctgttc aattgccttg aatgcaaact cgagggctgc ttccgccgag
      361 ggaaggtttg gcgcgaagcc gccttcatca cccacattgg tattatggcc ggccttcttc
      421 agagaggctt ttaaggtgtg aaagacttcg gcgcccatgc gcagagcttc cttgaatgac
      481 ggcgcgccta ccgggaggat catgaattcc tggaagtcga ttggattgtc ggcgtgggcg
      541 ccgccattga tgatgttcat catgggcacg ggaagtgtgc gcgccacagt gccgccgacg
      601 tagcggtaga gcggctggcc gcaagcttcg gcggcggccc ttgcggtggc gagcgagaca
      661 ccgagaacgg cgtttgcacc gaggcgggat ttgttgggcg tgccatcaag tgcaatcatg
      721 gcgcggtcaa cagcgatctg gtcttcggcg tccatgtcta tgagggtttc aaaaatttcg
      781 ccgttcacgg cggccaccgc attgagcacg cctttgccgc tatagcgctt tttgtcgcca
      841 tcgcggagtt ccatggcttc gtgagccccg gtggaagcgc cggatggcac ggccgcacgg
      901 ccgaaggaac catcctcgag aatgacgtcc acctcaacgg ttgggttgcc gcggctgtca
      961 agaatttcgc gggcggtaat gtcaagaatg gcggtcatgg ggctctccgg atgaggttcg
     1021 gggggcttat ggcgcggagt tgtgttgatt gcaagaacgg gccgtcagtt tgcctcccga
     1081 atggataagt tgaccggcaa gtgctgacgc cgatatgtcg acggtgttac tcccaccatt
     1141 cgtccaaata gccggcgaaa actcgcaggc tcggcgtagc cgacttcggc cgcgacttca
     1201 tccaggggca tttcgcttgt ttcaaggagc tgtttggcct cttcaatacg gactgtcggc
     1261 acgtaatcca tgggcgacat accagtagca cgtttaaaac gcgtgtgaaa actgcgctcg
     1321 gtcagaccac agttcgacgc catggctgcc acaggattgg ctttcatgta gttgtcggcg
     1381 atccagagtt gggccgattt gaccatctcg tcgctgtgct ggcggcgcgc cgtcaatcct
     1441 gcgaagggca actggccctg accatgcgac tgcagcaaaa agactttggc cacgcggcgc
     1501 gcctcctccg gcccggcaaa gcggccaatg agataaagca gcaggtcata ccatgacgtg
     1561 gccccgcctg ccgtgatcag ccgatggcca attccagcag gcacaagaat ccgttccctg
     1621 cgcatgcgga ttttgggaaa gtttcgggac atcgtatcgc aatagcccca atggcttgtt
     1681 gcgtcttcgc catcgagcag tcctgtctgg ccaaggagca tggaaccgga gcagacggag
     1741 gccaggattg ccccttttgc atgcattgcc cgaag
//
LOCUS       DAORVN010000514         1772 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_75923_length_1772_cov_2.976704, whole genome shotgun sequence.
ACCESSION   DAORVN010000514 DAORVN010000000
VERSION     DAORVN010000514.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1772)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1772)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1772
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75923_length_1772_cov_2.976704"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..720)
                     /locus_tag="PKV23_09700"
     CDS             complement(<1..720)
                     /locus_tag="PKV23_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012483684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HQX85752.1"
                     /translation="MAVKSAYIARKVTKADKADKSAGKNSGFSSEEELRAYKDMLLIR
                     RFEEKAGQLYGMGAIGGFCHLYIGQEAVVIGMQMAVKEGDQLITSYRDHGHMLAAGMD
                     PKGVMAELTGRRDGYSHGKGGSMHMFSREKQFFGGHGIVGAQVPLGAGLAFANKYRGN
                     DHVALTYYGDGAANQGQVYETFNIASVWKLPVIFVIENNSYAMGTAAPRSTAKSDHLG
                     ARGEAFGIPSEQVDGMDVRAVK"
     gene            complement(834..1130)
                     /locus_tag="PKV23_09705"
     CDS             complement(834..1130)
                     /locus_tag="PKV23_09705"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HQX85753.1"
                     /translation="MRISKFDSVVTCACLGLLGFLGWHAQEGPRGFAYNGKLTAQSRQ
                     LSDELAAIQKQRNDFETRVALLRPESVDPDMLDEMARSTLDVAGPYDLIVLDSP"
     gene            1237..1653
                     /locus_tag="PKV23_09710"
     CDS             1237..1653
                     /locus_tag="PKV23_09710"
                     /inference="COORDINATES: protein motif:HMM:NF014173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HQX85754.1"
                     /translation="MLSSSRFIVAIHALSVLARHANKGPVCSSTIATSVDTNPVVIRR
                     LMAELEKAKLVRATAGRCGGFELERNADGITLADIYQAVEDETVFRMHKTDPNSKCPI
                     ACQMGKILGAPLRAAESALSSSLARTTLKEVAEAVH"
BASE COUNT          349 a          490 c          556 g          377 t
ORIGIN      
        1 cttcacggcg cggacatcca tgccgtccac ctgttcggag ggaatgccga aggcttcgcc
       61 gcgggccccc agatggtctg acttcgcggt ggaacgcggg gcggcggtgc ccatggcgta
      121 gctgttgttt tcgatcacga agatgacggg gagtttccag acagaggcga tgttgaaagt
      181 ctcatacacc tggccctggt tggcggcgcc gtcgccgtaa taggtgagcg ccacatggtc
      241 attgccgcgg tatttgttgg cgaaagccag ccctgccccc aggggcacct gggcgccgac
      301 gatgccgtgg ccgccgaaga actgtttttc ccggctgaac atgtgcatgg agccgccctt
      361 gccgtgggaa tagccgtccc tgcggccggt gagctcggcc atgacgccct tggggtccat
      421 gccggcggcc agcatatggc cgtggtcgcg gtaggaggta atgagctggt cgccttcctt
      481 caccgccatc tgcatgccga tgaccacggc ttcctggccg atatagagat ggcagaaacc
      541 gccaatggcg cccatgccat agagctggcc ggctttttcc tcaaagcgcc gaataagcag
      601 catgtccttg taggcgcgga gttcttcttc ggaggaaaag ccgctgtttt tgccggctga
      661 cttgtcggcc ttatcagcct tggtcacttt gcgcgcgata taggctgatt tcaccgccat
      721 aaactcctcc cggcatgctt tttggcatga ttcatagcat taagcggttg agatagcttg
      781 cttttagtgc cgttaactga atttcagttt acagcgcgga tttcattgag gaattatggt
      841 gaatccagca cgatgaggtc gtagggacca gccacatcga gggtggacct cgccatttcg
      901 tcgagcatgt cgggatcgac ggattccggc cggagaagag cgacgcgagt ctcgaaatcg
      961 ttgcgctgct tctggattgc ggccagttca tcgctcaact gccgggattg ggccgtcagt
     1021 tttccgttgt aggcgaagcc gcgagggcct tcctgggcat gccagcccag gaacccgagc
     1081 aagccgaggc aggcacaggt gaccacgctg tcaaatttgg agattcgcat ggcttgcaga
     1141 ctcgggcgta tcacttaagg tttggtaaat gcactgcctt atgcgggctt gctttttgcc
     1201 acaaacgata cgatgtaagg gcaaaggtca aatgagatgt tatcgagcag ccgatttata
     1261 gtcgcaattc acgccctaag cgtgctggcg cgccatgcca acaagggccc ggtgtgttcg
     1321 tcgactattg caaccagcgt cgataccaac ccagtggtga tccgcaggct gatggcggaa
     1381 ctggaaaagg ccaagctggt gcgcgccacg gcgggccgct gcggtggatt tgaactcgag
     1441 cgcaacgcag atggcatcac actggctgat atctatcagg ctgtggaaga cgagaccgta
     1501 ttccgcatgc acaagaccga tcccaattcc aaatgcccga ttgcctgcca gatgggtaaa
     1561 atcctcggtg cgccattgcg tgccgctgaa agtgcattgt catcgtcgct ggcacggacg
     1621 acgctcaagg aagtggccga agcggtccac tgaatgtgcc tattttagaa tcctgaaatc
     1681 accgatggcg atgcttgtgg cgtcggcggg gccggcgatg cggagccgga cccgcgcgcc
     1741 ggggacgaag gccatggcgc cgcggcgggg cg
//
LOCUS       DAORVN010000515         1772 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_75931_length_1772_cov_2.831101, whole genome shotgun sequence.
ACCESSION   DAORVN010000515 DAORVN010000000
VERSION     DAORVN010000515.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1772)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1772)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1772
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75931_length_1772_cov_2.831101"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(45..833)
                     /locus_tag="PKV23_09715"
     CDS             complement(45..833)
                     /locus_tag="PKV23_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011500913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HQX85755.1"
                     /translation="MIKKPSRQLAIDGNVILKDALKDIGRHIEQPARRAFLQRSLTLG
                     GLSLLSGCAIVDESSVENALMKVSRFNDKVQGWLFDPKRLAPTYPDSMITRPFPFNAY
                     YDEDEIREVDEESYRLEVSGMVADKHAWKLAELRAMPQTDQVTRHICVEGWSAIGKWG
                     GVPFASFLRKIGADLSAKYVGFKCADDYYTSIDMPTALHAQTLLTLTYDSQPLPPRYG
                     FPMKLRMPTKLGYKNPKHIQAIFVTNTYPGGYWEDQGYNWFGGS"
     gene            complement(846..1442)
                     /locus_tag="PKV23_09720"
     CDS             complement(846..1442)
                     /locus_tag="PKV23_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011661930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HQX85756.1"
                     /translation="MDNDSGLIHPLWVRVTHWINAVAVVILVASGWKIYNASPLFGFR
                     FPETITLGGWLGGAIQWHFAAMWLLFANGLVYLACNAVSGRLLNKFFPLSPRAVLADA
                     LAAMKGQLSHADLRHYNAVQRSAYLFVMLDSVLLVLSGLVLWKSVQFPILRELLGGYE
                     AARRVHFLAMAALVAFAGIHLLMVALVPRTLLAMIRGR"
BASE COUNT          373 a          490 c          539 g          370 t
ORIGIN      
        1 gacggcatcg atgccgggta ttcaggacag tcgagcggga ttgctcagct gccgccgaac
       61 cagttgtacc cctggtcttc ccagtagccc ccggggtagg tgttggtgac aaaaatcgct
      121 tgaatgtgct tcgggttctt gtagccgagc ttggtcggca tgcgcagctt catcgggaaa
      181 ccgtagcgcg gcggcagtgg ctggctgtcg taggtgagcg tcagtagtgt ttgcgcgtgc
      241 agtgcggttg gcatgtcgat gctggtgtaa tagtcatcgg cacatttgaa gccgacgtac
      301 ttggcactga gatcggcgcc gattttgcgg agaaagctgg cgaacgggac gccgccccat
      361 ttgccgatgg cgctccagcc ctcgacgcaa atatggcggg tgacctgatc ggtctgtggc
      421 atggcacgta actcggcaag cttccacgca tgtttgtcgg ccaccatgcc actcacctca
      481 aggcgatagc tttcctcgtc gacttcccgg atttcgtctt cgtcgtagta ggcattgaac
      541 gggaaggggc gggtaatcat cgaatcggga taggtcggcg ccagacgctt cgggtcgaac
      601 agccagccct ggaccttgtc gttgaagcgg gaaaccttca tcaacgcgtt ctcgacgctg
      661 ctttcatcga caatcgcgca accgctcaac agcgataatc cgccaagggt cagggagcgc
      721 tgcaagaagg cgcggcgtgc cggttgttca atatggcgcc cgatatcctt gagtgcgtcc
      781 ttgagtatga cgttgccatc gatggcgagt tgccgggaag gtttcttgat catgtctatt
      841 gttccctatc gtccacggat cattgccagc agcgtgcgtg gcaccagtgc caccatcagc
      901 agatgaatgc cggcgaatgc caccagcgcg gccattgcca ggaagtgcac ccgccgcgcg
      961 gcttcgtaac cgccaagcag ttcgcggaga atcgggaact gcaccgattt ccacaacacc
     1021 aggccggaca gcacaagcag caccgaatcg agcatcacga acagataggc gctccgctgt
     1081 accgcgttgt agtggcgcag gtcggcatgg gacaactgtc ctttcatggc ggcgagggca
     1141 tccgcaagca cggcgcgtgg cgacaacggg aaaaacttgt tcagtagtcg cccgctgacg
     1201 gcgttgcacg cgaggtaaac cagaccgttg gcgaacagca gccacatcgc cgcgaaatgc
     1261 cactgaatcg cgccgcccag ccatcccccc agggtgatgg tttcaggaaa gcgaaagccg
     1321 aacaaaggcg atgcgttgta gatcttccag ccgctcgcca ccaggatgac gaccgcgaca
     1381 gcattgatcc aatgcgtgac gcgcacccag agcgggtgaa tgaggccgga gtcgttgtcc
     1441 atgtcgttcc tttcagcttt tccgcgtttt tatgtgcggg tctgcaaggt agttcgctgg
     1501 caagtggcga atggttacaa gctcaggcag aatttgtttt ccccaagaca gggcaagaat
     1561 atttttcggc cgctgtaacc aaaggcgtca atgcaacgaa taaatagaga agccgggttt
     1621 cgtgtgaagg aagatcgcct taaggatcaa ctggtgcgcg gactggccgg cgacaacgtt
     1681 gcctatcagg tgtttctgac ggaattaagc gcgcatctgc gtgccttcct gcgcaagcgc
     1741 cttgtccgct tatcagatga agtcgaggat ct
//
LOCUS       DAORVN010000516         1770 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_76062_length_1770_cov_3.275802, whole genome shotgun sequence.
ACCESSION   DAORVN010000516 DAORVN010000000
VERSION     DAORVN010000516.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1770
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76062_length_1770_cov_3.275802"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="PKV23_09725"
     CDS             <1..250
                     /locus_tag="PKV23_09725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85757.1"
                     /translation="ELVVESRNQWNQRTDPNMKSSRCLRASGCIRHCVTESSSQRMWA
                     QSGSVGPGLACIANGAFHALLADEEIRAFISQPERPMK"
     gene            complement(361..708)
                     /locus_tag="PKV23_09730"
     CDS             complement(361..708)
                     /locus_tag="PKV23_09730"
                     /inference="COORDINATES: protein motif:HMM:NF025120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HQX85758.1"
                     /translation="MAKKLEQIMAALPAKRRGKIEARVAELATLKDLRQAVAQTQEEL
                     AATLGVGQDTISRLEKRSDMLLSTLRRYVEAMGGKLELVAKFPDRPPLVIEHLADETS
                     ARRAVSAQGKAHA"
     gene            complement(692..1078)
                     /locus_tag="PKV23_09735"
                     /pseudo
     CDS             complement(692..1078)
                     /locus_tag="PKV23_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018429562.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(1260..1586)
                     /locus_tag="PKV23_09740"
     CDS             complement(1260..1586)
                     /locus_tag="PKV23_09740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HQX85759.1"
                     /translation="MAPDDIYLSVQTIGEIRRGLESIRHRGDHEQADRLEVWLEALLA
                     DYSDRILGFDLDCAQVWGRLMSPNPQHAIDKQIAAIALIHDLTVVSRNTRDFGWKGVR
                     WMNPFS"
BASE COUNT          344 a          550 c          505 g          371 t
ORIGIN      
        1 tgagcttgtc gttgaaagca gaaatcagtg gaatcagagg acagacccca atatgaaatc
       61 ttctcgttgt ctgcgtgcgt cggggtgcat ccggcattgt gtgacagaat cttcctccca
      121 acgaatgtgg gctcaatccg gatctgtcgg ccccggcctg gcttgcatcg cgaacggagc
      181 attccatgca ctactggctg atgaagagat aagggccttc atcagccagc ctgaacgccc
      241 gatgaaataa ggcttttaac gaaggatact cgttcgaagc gccgccaagg agccgccttg
      301 gggggaatcc gggtgggact tcgggcgggg catccttgga cgttcgcagg ccttcagcga
      361 tcaggcgtgt gccttgccct gcgcgcttac ggcgcgccgc gctgaagtct cgtcagccag
      421 atgttcaatg accaggggtg gccggtcggg gaatttggcc accagttcca gcttgccgcc
      481 catcgcctcg acgtagcggc gcagcgtcga cagcagcatg tcgctgcgct tttccagtcg
      541 cgaaatcgtg tcctggccga cacccagtgt cgcggccaat tcttcctgcg tctgcgccac
      601 ggcctggcgc aaatccttga gcgtcgccaa ttccgctacg cgcgcctcga tcttgccccg
      661 gcgcttggcg ggcaaggcgg ccatgatctg ctcaagtttt tttgccatgt ctgtgttcct
      721 tctctacttg ctgctgatcg gccaaatgct ggtcaaagcg cgtgtcggcg acggcaatca
      781 gccgcttgta aaaccgccgt tgatctgccc ctgccttgtt gccgccgacc agcaaaatcg
      841 cctggcgttt ggggtcgaac gcgaaagcca cacgccaaac ctcaccttcc caactgaaac
      901 gcagttactt catgttggca tgccgggaac ctttcagacc atccaccgtg gggcggccaa
      961 gattgggccc gaactcgcgc aacaacaggg cgtgcgccag caactcatcc tgcaacgcct
     1021 gggcgtactc ccgcatctcg gcatcgaagt cgtcgtgaaa gagaatatcc caactcatga
     1081 ccgaattatg gacttaagtg catatggatt gcaatacata tcacggtcgt cgtcggattc
     1141 ccgtggacag ctccgtcatg gggcttgctc aatgcctccc tacccctatt cgctccagga
     1201 gcgcgggctg ctgaatctcg acgggtatca gcttcttgaa ggtcaacacc cctgccaggc
     1261 taactaaaag gattcatcca gcgcacgccc ttccagccga aatctctggt gttgcgggac
     1321 acgaccgtca ggtcgtgaat cagcgcaatg gcggcgatct gtttgtcgat ggcgtgctgg
     1381 ggattgggtg acatgagcct gccccacact tgagcacagt cgaggtcgaa ccccagaatc
     1441 cggtccgaat agtccgccag cagcgcctcc agccagactt caagccggtc ggcctgctcg
     1501 tggtcaccac ggtggcggat actctccagc ccgcgtcgga tctcgccaat ggtctggacc
     1561 gacagataga tgtcatccgg cgccaatcct gcaaaaaagc cgtcaccccc ggattggcgc
     1621 gcccaccttt gtgcaactca ctgatgactt tggtatcgat caggtgcatc agggcggcct
     1681 gaagtcgaag tcttcgtcgg tccctacgtc gggcatcgcg gccagcacat ccttgaggct
     1741 gcgacgtgcc ggtcgggtga gcgccgcccg
//
LOCUS       DAORVN010000517         1770 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_76084_length_1770_cov_2.602915, whole genome shotgun sequence.
ACCESSION   DAORVN010000517 DAORVN010000000
VERSION     DAORVN010000517.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1770
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76084_length_1770_cov_2.602915"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            63..1328
                     /locus_tag="PKV23_09745"
                     /pseudo
     CDS             63..1328
                     /locus_tag="PKV23_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590732.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
     gene            1337..>1770
                     /locus_tag="PKV23_09750"
     CDS             1337..>1770
                     /locus_tag="PKV23_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008835210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HQX85760.1"
                     /translation="MTNRTYYAPQGGLPPQATLLTDRAVFTESYAVIPKGVLRDITVS
                     YFPFWEKTRAWVLSRPLSGFAETFSQYIMDVKPEGGSDKPEPDPHAEGVLFVVEGELN
                     LTLSGKSHVMQPGGYAYVPPGAKWSLRNKGAKSVKFHWIRKA"
BASE COUNT          320 a          517 c          602 g          331 t
ORIGIN      
        1 ggctgcgaat acaagccgaa gggcacgacg gttgacggcc tgggctggat gaagatgatg
       61 acatgacacc ggaagccacg cgcaaattgc tgcgttccat gttcgatgcg gcggtcgctg
      121 cggcgatgtc ggcgcaatgc gtgccgcaga acctgccggc gaagccgaag ggccggacga
      181 ttgtcgtcgg caccggcaag gcttcggcgg ccatggcgca ggtgcttgag agatcctggg
      241 atggcccgct ctccggcctg atcgtcacgc gctatggcca tgcggttccc tgcaggcaga
      301 ttgaaatcgt cgaagcggcc catccggttc ctgatgatgc gggcactatg ggcgcgcggc
      361 ggatgctggc catggtgcag gggctcagca aggatgatct cgtcattgcg ctgatctcgg
      421 gcggcggctc ggcgctgctc agccttccgg cggaggggat taccgttgaa gacaagcgcg
      481 ccgtcaatcg tgccctgctc aagagcggcg cgccgatttc ggaaatgaat tgcgtgcgac
      541 cgcatagcta ggccatcaag ggtgggcggc ttgcggcggc ggcgcatccg gcccgcgtgg
      601 tgagcctggt gatttccgat gtgccgggcg atgatctcgc cgccgttggc tctggcccca
      661 cggtggctga tcccacgacc ttcgcccagg cccgcgccat cattgcgaaa tacaaaatcg
      721 atgcgcccgc ctctgtcatc cgccatctcg agcaggcgat tgatgaaacg ccgaagcccg
      781 gtgatccgcg gcttgttggc gtcgaaacaa agctcatcgc ctcgccgcag aaatcgcttg
      841 aagcagcggc ggccatcgcg cgcaaggccg gcatcacccc gcttattctt ggcgattcca
      901 tcgagggcga ggcgcgcgag gttggctacg tgatggcggg aattgccctg cagtcgcggc
      961 gtttcgggca gcctttgcca gcaccttgcg ttctaatctc gggcggggag acgacggtca
     1021 cggtgcgggg cacaggtgcc ggcgggcgca atgtggaatt tcttctggcg ctggcgctga
     1081 agcttgacgg ggccgaaaaa atcatggcgc ttgcggccga cacggatggg gttgacggtg
     1141 cgcgtgaagt ggcgggcgct ttcatcacgg cggacacgct tgcccgggcc cgggccatgg
     1201 ggattgatcc ctgggcttcg cttgccaata atgacgggca tgggtttttt gaaaagctgg
     1261 gcgaccagat catcaccggg ccgacgctga ccaacgtcaa tgactttcgc gccgtgtaca
     1321 taggatagga tcagacatga ccaaccgcac ctattatgca ccccagggcg gattgccgcc
     1381 gcaggccacg ttgctcacgg accgcgccgt gttcacggaa tcctatgcgg tgatccccaa
     1441 gggtgtgctg cgcgatatca cggtgagcta ttttcccttc tgggaaaaga cgcgggcctg
     1501 ggtgctgtcg cggcccctgt cgggatttgc cgaaaccttc tcgcaataca tcatggatgt
     1561 gaagccggag ggcggcagcg acaagccgga gcccgatcca cacgcggaag gcgtgctgtt
     1621 tgtggtggag ggggaactca atctcacgct gtccggcaaa tcccatgtga tgcagcccgg
     1681 cggctatgcc tatgtcccgc cgggagcgaa atggagcttg cggaacaagg gcgcgaagtc
     1741 cgtgaagttc cactggatcc gcaaggctta
//
LOCUS       DAORVN010000518         1767 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_76341_length_1767_cov_2.084696, whole genome shotgun sequence.
ACCESSION   DAORVN010000518 DAORVN010000000
VERSION     DAORVN010000518.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1767
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76341_length_1767_cov_2.084696"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1033
                     /locus_tag="PKV23_09755"
     CDS             <1..1033
                     /locus_tag="PKV23_09755"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HQX85761.1"
                     /translation="EDKTPADKLSDEDLRKRLDGIRELMAGNELSRRTEQALRKKLRT
                     EREILRNRVAMAEPPKPAKPSAGNEEAPKPSKFDRKDYAFNLEIVLNDRRPSEELQDY
                     ELRRRLEVYRQYDRDDRYSADQRAYWREVRQRDEALLQRRLLRERRQRQAELASRYED
                     NEYDIELGEGYDPDQRDDVYAAEVDDEELEEVLVAAPRREFDRRYTVEEVEASPDLRE
                     ALPRIEIDTVRFGFNEAFVRPEEVGNLDRIGEIMERVLSKHPRERFLIEGHTDAVGSD
                     AANLALSRERAAAIKKALTTYYVISPENLETVGYGERYLKIPTAEAEQENRRVSVSRA
                     TSVFGELED"
     gene            1107..1529
                     /gene="ndk"
                     /locus_tag="PKV23_09760"
     CDS             1107..1529
                     /gene="ndk"
                     /locus_tag="PKV23_09760"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012112268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HQX85762.1"
                     /translation="MAIERTFSIIKPDATRRNLTGAINAKIEAAGLRIIAQRRTKWKQ
                     ADAKKFYAVHAKRPFYKDLVKFMTSGPIVMQVLEGNNAVARYREIMGATNPANADAGT
                     IRKDFAESIEANSVHGSDSAENAKKEIKLCFKAAEIVG"
     gene            1534..>1767
                     /locus_tag="PKV23_09765"
     CDS             1534..>1767
                     /locus_tag="PKV23_09765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85763.1"
                     /translation="MWVALYIAAIVLVNWLFTAVAPWSTVLSDLYLANVVVGFVFVLR
                     DYAQREVGHKVLLATLFAGIATYVMVDPAIAIAS"
BASE COUNT          397 a          533 c          515 g          322 t
ORIGIN      
        1 tgaggataaa acgcccgccg acaagctttc cgatgaagat ttgcgcaagc gccttgatgg
       61 cattcgcgaa ctgatggccg gcaacgaact gtcacgcagg accgagcagg cgctccgcaa
      121 gaagctgcgg accgagcgcg agatcttgcg taaccgtgtg gccatggcag agccgcccaa
      181 gccagccaag ccgtcagccg gcaatgagga agcgcccaag ccatcaaagt tcgacaggaa
      241 ggattatgcc ttcaacctcg agatcgtgct gaacgaccgc aggccgtctg aagagctgca
      301 ggattacgaa ctccgccgcc gtctggaagt gtatcgtcaa tatgaccggg acgatcgtta
      361 cagtgccgat cagcgcgcct attggcggga agtcaggcag cgtgacgaag cgcttctcca
      421 gaggcgcctg ctgcgcgagc gccgccagcg ccaggctgag cttgcctccc gctatgagga
      481 caacgagtat gatatcgaac tgggcgaagg ctatgatccg gatcagcgcg acgacgtcta
      541 tgccgccgaa gttgacgatg aggaactgga ggaggtgctc gttgcagcac ctcgccggga
      601 attcgaccgc cgctacacgg tggaggaagt ggaagcctca cctgatctcc gcgaggcttt
      661 gccgcgcatc gagattgata ccgtgcgctt tggcttcaat gaagccttcg tgcgcccgga
      721 ggaggtgggc aaccttgacc gtatcggcga aatcatggaa cgcgtcctca gcaagcatcc
      781 gcgcgaaagg ttcctgatcg agggccatac cgatgcggta ggctcagacg ccgccaactt
      841 ggcgctgtcg cgcgaacgcg ccgcggccat caagaaggcg ctcacgacct attacgtcat
      901 ctctcctgaa aaccttgaaa ccgtgggtta tggcgagcgt tacctgaaga tccccaccgc
      961 cgaggcggag caggaaaacc gccgggtgtc ggtgagccgc gccacctccg tattcggtga
     1021 actcgaggat taacctcttt cccttgcaag ttacttgggc cgcccctaaa aggcggccca
     1081 attcatatct aaacataggg aaaatcatgg ccatcgaacg taccttttcc atcatcaagc
     1141 cggacgcaac acggcgcaat ctgacgggcg ccatcaacgc caagatcgag gcggcgggcc
     1201 tgcgcatcat tgcccagcgc cgcaccaagt ggaaacaggc cgacgccaaa aaattctacg
     1261 ccgttcatgc caagcgcccg ttctataagg accttgtgaa gttcatgacc tccggaccca
     1321 tcgtcatgca ggtgctggaa ggcaacaacg cggtcgccag ataccgcgaa atcatggggg
     1381 ccaccaaccc ggccaatgcc gatgccggaa ccatccgcaa ggatttcgcc gagtccatcg
     1441 aagccaactc ggtgcatggc tccgatagcg ccgagaacgc caagaaggaa atcaagcttt
     1501 gcttcaaggc cgctgagatc gtcggctgaa gccatgtggg tcgccctcta cattgctgct
     1561 atcgtgctgg tgaactggct gttcaccgcc gtggcaccct ggagcacggt cctgagcgac
     1621 ctctatctgg ctaatgttgt agtgggcttc gtctttgtcc tgcgcgacta tgcacagcgc
     1681 gaagtcggcc acaaggttct attggcgaca ttgtttgccg gcatcgccac ctatgtcatg
     1741 gtggaccccg ccattgcaat cgccagc
//
LOCUS       DAORVN010000519         1765 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_76459_length_1765_cov_3.007602, whole genome shotgun sequence.
ACCESSION   DAORVN010000519 DAORVN010000000
VERSION     DAORVN010000519.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1765
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76459_length_1765_cov_3.007602"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..444)
                     /locus_tag="PKV23_09770"
     CDS             complement(<1..444)
                     /locus_tag="PKV23_09770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85764.1"
                     /translation="MNLFEAIGVGTIINAKGPATRLSGGIMRDEVAQAMSEASQHCVE
                     IAELQAAASRIIAETTGAEAGYVASGASACLLLATAACMTGLDPGRMGRLPDAKGMND
                     EVIMVRSQRNFYDHAVRAAGARIVEVGLPDRYAGAGVRDAEAWEID"
     gene            620..>1765
                     /locus_tag="PKV23_09775"
     CDS             620..>1765
                     /locus_tag="PKV23_09775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008875444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HQX85765.1"
                     /translation="MALRGTNQEFGRPYNRRIVLEAIRQYGPIARADIAGRVGLTVQT
                     VSNIIRELEEQSYIAGVRDAPKSRGYPATSLSINPEGGYAIGIDITPLGLEAALINLA
                     GEVVAREQRKLTHPAPEAAFREIFALVKEFRSIKHGQRMLGIGFAMPGPFDVESMSFV
                     GPTTLEGWKGVDVQRRLAEGANLPAFIEVDHAAAALGERLYGAGRGFRDFYYLYFGVG
                     LGGCMVHEGHPLRGSFRNAGEIGHLPLVPDGEPCPCGNRGCLERYLSLEAHERRTKQI
                     GVEGWIAETVPLFRSAIVTIENLFDPETIIIGGLANESLLARLVAAVQPLNNSISKRE
                     NRKAPRLMHSTVGNDAVLRGAAALAVAGVLSPRFGIQFTEASASPPSD"
BASE COUNT          362 a          553 c          490 g          360 t
ORIGIN      
        1 gtcaatctcc caggcttccg cgtcccgcac cccggcccct gcgtaacggt cgggcaaccc
       61 tacttcgact atccgcgccc cggcagcgcg aaccgcgtga tcgtagaaat tacgctggct
      121 gcgcaccata atgacttcat cattcatccc ttttgcatcc ggcagccgtc ccatgcggcc
      181 ggggtcaagc cctgtcatgc atgcggcggt ggccagcagc aggcaagctg acgcgccaga
      241 agcaacgtaa cctgcctctg caccggttgt ttcggcgatt atccgtgaag cggcggcctg
      301 aagttcggcg atctcgacac aatgctgtga ggcttcgctc atagcctgcg ccacctcgtc
      361 ccgcatgatg ccgccggaca ggcgcgttgc aggtcccttg gcatttatga tggtgccaac
      421 accgatggct tcaaacaggt tcaagaattc accatgcgat cactctatta ctctctcatc
      481 cacagataat gcccccgccc acgcgttcga aaatcatgac agaaattcaa tgtcaaaaat
      541 aaagtcagta tttttgaatt ccatttgatt ttatatttat ctcgtgcttt cctgaaaaaa
      601 gcaaacaaag aaccacaaaa tggcactaag gggaacgaac caggaatttg gaaggccgta
      661 taatcggcgg attgtgctcg aagccatacg ccagtacggg ccaattgcgc gggccgatat
      721 tgccggacgc gtgggcctga cggttcagac tgtttccaac attatccgcg aattggaaga
      781 acagagctat attgctggcg tcagggatgc tcccaaaagc cgcggctatc ccgccaccag
      841 cctcagcatc aatccggaag gcggctatgc catcggcatt gacattacgc ccttggggct
      901 tgaagcggcc ctgatcaatc tggccggaga agtggtggcg cgcgaacagc gcaagcttac
      961 ccaccccgcc cccgaagcgg ccttccggga aatattcgcg ctggtgaagg agttcaggag
     1021 catcaaacac ggccagcgta tgctcggcat cggctttgcc atgccaggcc ccttcgacgt
     1081 ggaatccatg agctttgtcg gccccaccac gctggaaggc tggaaaggcg ttgatgttca
     1141 gcgacgcctg gccgaaggcg caaatctgcc ggcctttatc gaagtggatc acgccgccgc
     1201 cgcgcttggt gaacggctct atggcgccgg ccgcggtttc cgtgacttct attatctgta
     1261 tttcggcgtt ggcctcggcg gatgcatggt ccatgagggc catcccctgc gcggcagttt
     1321 ccgcaatgcc ggagagatcg gccatctgcc gcttgtcccc gatggcgagc cctgcccctg
     1381 cggaaaccgc ggctgcctcg aacgttacct gtcgcttgaa gcccatgagc ggcgcacgaa
     1441 acagattggc gttgaaggct ggatagccga aaccgtgccg ctgtttcgct ccgccatcgt
     1501 tacgattgaa aatctcttcg atcccgaaac gattatcatc ggcgggcttg ccaacgagtc
     1561 cctgctggcg cgccttgttg ccgccgtcca gccgctgaac aattccattt caaagcggga
     1621 aaaccgcaag gccccgcgcc tgatgcattc gacggtgggc aacgacgccg tcctgcgggg
     1681 cgccgccgct cttgccgtgg cgggcgtcct gtcgccgcgc ttcggcatcc agttcacgga
     1741 agccagcgcc agcccgccga gcgat
//
LOCUS       DAORVN010000520         1762 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_76744_length_1762_cov_2.887522, whole genome shotgun sequence.
ACCESSION   DAORVN010000520 DAORVN010000000
VERSION     DAORVN010000520.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1762)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1762)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1762
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76744_length_1762_cov_2.887522"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1762
                     /gene="scpA"
                     /locus_tag="PKV23_09780"
     CDS             <1..>1762
                     /gene="scpA"
                     /locus_tag="PKV23_09780"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HQX85766.1"
                     /translation="MSRIPNFSEIPLDLSSRRKPESSEADSGSRRIDRIWETPEGIKI
                     AAGYGPDESYAESYPGIAPFLRGPYPTMYTTQPWTIRQYAGFSTAEDSNAFYRRNLAA
                     GQMGLSIAFDLATHRGYDSDHPRVAGDVGMAGVAIDSIYDMRTLFDGIPLDRMTVSMT
                     MNGAVLPILALFIVAGEEQGVPPEKLSGTIQNDILKEFMVRNTYIYPPQHSMRIVSDI
                     FKYTSTHMPKFNLISISGYHMQEAGATADLELAYTLADGFEYAKAGVDAGLDIDRFAP
                     RLSFFWAIGMNFFMEVAKMRAARAIWATLMQRFSPKDERSLILRTHCQTSGWSLAAQD
                     VFNNVTRTCIEAMAATQGGTQSLHTNSLDEALALPTDFSARIARNTQLFLQQEAGTCR
                     VIDPWGGSHFMEKLTADLMAKAWAHIGEIEKIGGMAKAIEAGIPKMRIEEAAARTQAR
                     IDAGEQTVVGVNKYRNSDETKIDILKVDNSAVRASQIEKLTRLKAERDPLTCQEMLDR
                     LSLVAKTGDGNLLAAAVDAARAKATVGEISLALEKVWNRHVATTRVTKGIYIASSRDL
                     QKIEMAKQAVEAFKEADGQPP"
BASE COUNT          373 a          525 c          535 g          329 t
ORIGIN      
        1 atgagccgca ttccaaactt ctcagagatc cccctcgatc tgtcatcccg gcgaaagccg
       61 gaatcatctg aagcggattc cggctctcgc cggattgacc ggatttggga aacgcctgaa
      121 ggcatcaaga tcgctgcggg ttatgggccg gacgaaagtt atgctgaatc ttatcccggc
      181 atcgcgccgt tcctgcgtgg cccctacccc accatgtaca caacgcagcc ttggaccatc
      241 cggcaatacg ccgggttttc gacggcggag gattccaatg ctttctaccg ccgcaatctg
      301 gcggcggggc agatggggct ttccatcgcc ttcgacctgg ccacgcaccg gggctatgac
      361 agtgaccatc cgcgcgtcgc cggtgacgtc ggcatggcgg gcgtggcgat tgattccatc
      421 tacgacatgc gcacgctgtt cgacggcata ccgctggaca ggatgacggt gtcgatgacg
      481 atgaatggcg cggtgctgcc gatcctggca ctgttcatcg tggcaggcga ggaacagggc
      541 gtgccaccgg aaaagctttc cggcaccatc cagaacgaca tcctcaaaga attcatggtg
      601 cgcaacacct atatctatcc gccgcagcac tccatgcgca tcgtctccga cattttcaaa
      661 tacacctcca cgcacatgcc gaagttcaat ttgatttcga tttccggcta tcacatgcag
      721 gaagcgggag ccaccgccga tcttgagctc gcctacacgc tggccgatgg ttttgaatat
      781 gccaaggccg gcgttgatgc gggattggac atcgacagat ttgcgccgcg gctttccttc
      841 ttctgggcca tcggcatgaa cttcttcatg gaagtggcca agatgcgcgc cgcgcgcgct
      901 atctgggcca cgctgatgca gcggttctca ccgaaggacg agcgctcgct gatcctgcgc
      961 acccattgcc agacgtcggg ctggagcctt gcggcccagg acgtgttcaa caatgtgaca
     1021 cgcacctgca tcgaggcgat ggcggcgacg cagggcggca cgcaatcgct gcacaccaat
     1081 tcgctcgatg aggccctggc actgcccacg gatttttcag cgcggattgc ccgcaacacg
     1141 cagctcttcc tgcagcagga agccggcacc tgccgggtca tcgatccgtg gggcggaagc
     1201 cacttcatgg aaaagctcac ggctgatctc atggcaaagg cttgggcgca tatcggcgag
     1261 attgaaaaga tcggcggcat ggccaaggcc atcgaggccg gcattcccaa gatgcggatc
     1321 gaggaagcgg cggcccgcac ccaggcgcgc attgatgcgg gcgagcagac tgtcgtcggc
     1381 gtaaacaagt atcgaaattc cgatgaaacc aagatcgaca tcctgaaggt ggacaattct
     1441 gccgtgcgtg ccagccagat tgaaaagctc acacgcctga aggcggaacg cgatccgctg
     1501 acatgccagg agatgctgga ccgcctgtcg ctcgttgcaa agaccggtga cggcaatttg
     1561 ctggcggcgg cggttgacgc ggcgcgggca aaggcgacgg tgggtgaaat atcgctggcg
     1621 cttgaaaaag tctggaaccg gcatgtagcg accacgcggg tgaccaaggg catttatatc
     1681 gcctcgtcgc gcgacctgca gaagatcgaa atggccaaac aggcggtgga ggccttcaag
     1741 gaggctgatg gccagccgcc gc
//
LOCUS       DAORVN010000521         1761 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_76831_length_1761_cov_2.689918, whole genome shotgun sequence.
ACCESSION   DAORVN010000521 DAORVN010000000
VERSION     DAORVN010000521.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1761)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1761)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1761
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76831_length_1761_cov_2.689918"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1013)
                     /locus_tag="PKV23_09785"
     CDS             complement(<1..1013)
                     /locus_tag="PKV23_09785"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HQX85767.1"
                     /translation="MRLLLIPVVCIVAMFFIGFPQSYAAGDAVATGTRVGGNETRTRF
                     VADISKTVNFSVYVLPDPYRVVIDLPDVTFDLPPGIGHKVRGLVSQYRYGLVEAGKSR
                     IVIDTHGPVLIEKSFLVEPQTGQPARLVVDLVKTTEQAFVKSYNAEQAKIQKDMAALS
                     VPPADTAVPEKKKILGIKRGKKTIVLDPGHGGIDPGAISRAGTKEKDVVLSFANVLRQ
                     ALLASGKYNVVMTRSDDRFVSLKNRVTVAREKNADLFLAIHADTVRGRDARGVTIYTL
                     SDKASDAEAEALAKRENRADIIGGLDLEAETEEITDILIDLAQRESKNHAMFFSKKAV
                     AQLR"
BASE COUNT          361 a          492 c          535 g          373 t
ORIGIN      
        1 cgcagctggg cgaccgcctt cttggaaaag aacatggcat ggttctttga ctcgcgctgg
       61 gcgagatcga tgaggatgtc agtgatttcc tcggtttcgg cctcaagatc gaggccgcct
      121 ataatgtcag cgcggttttc gcgcttggcc agggcttccg cttcggcgtc cgaagccttg
      181 tcggacaggg tgtagatcgt aacaccgcgg gcatcccggc ccctgacggt atcggcgtga
      241 atggcgagaa acaggtcggc gtttttttcc cgggcaacag tcacccggtt tttcaggctt
      301 acaaaacggt catcgctgcg cgtcatcacg acgttgtatt tgccgctggc cagcagggcc
      361 tggcgcagga cattagcgaa agaaagcacc acatccttct ctttcgtgcc ggcacggctg
      421 atggcgccgg gatcaatgcc gccatggccg ggatcgagga cgatggtttt cttgccacgc
      481 ttgatgccca gaattttttt cttttccggg accgccgtat cagccggagg gaccgacagg
      541 gcagccatgt ccttctggat tttcgcctgc tcggcgttgt aagacttgac gaaagcctgc
      601 tccgtggttt tcaccaggtc gacgacgaga cgggctggct ggccggtttg cggctccacc
      661 agaaacgatt tttcaatcag aacgggtccg tgggtgtcaa tgacgatgcg ggattttccc
      721 gcttcaacca ggccgtagcg gtactgagat accagccccc gcaccttatg gccaatgccg
      781 ggcggcaggt cgaacgtcac atccggcagg tcgataacaa cccggtaagg atcggggagc
      841 acatacacgc tgaaattcac ggttttggag atatcggcga cgaagcgggt gcgggtctca
      901 ttgccgccta cccgagttcc ggtggcaacg gcatctcccg ccgcatagga ttgcgggaag
      961 ccaatgaaaa acatggcaac aatgcacaca accgggatca acaggaggcg cataaactgc
     1021 ctcattcttc ccctttccag tgcgttgtgc ggtgcaatca tcgtgttttg gcccaatcgc
     1081 gggaaggtta gcggcgccgt ggttaaccga aggtttccgc gggcagattt ggctgggtgg
     1141 gatgagcgac cttgcaattg ggggcccttc tccctaaaga taaggggtct cttcggcaga
     1201 ctcgattcaa tcgtttgggt tgcaagagca aaaatttcgc ggcagagagg tccggagaac
     1261 ctgctggttc actccttcgt gccgcgtcta ggtaaggttc ccaagtcggg ggccttgtgg
     1321 gcttcccggg caaaggacat ccgcccgggc acgccgatga gtttaaccgt tcaggccatg
     1381 gtggcttgag caaagatcaa tgagggccat gatccaggac ttttcgattg cgcctccagc
     1441 cgctggaaac acagccagca gggagccgtt cgcatcgcaa aacgtcgtga catggatttg
     1501 gcccatttcg cgcaggctgg cccgtcgggc cgtctggttt ctcaagtccg gacatgggac
     1561 cttcgaagcc tcaccgccag ctgccgcaga ggatacaaga ccatgggcag caaaatgctc
     1621 gtcgatgcca cgcatgccga ggaaacacgc gttgtcgtgg ttcgcggaaa ccgcatcgag
     1681 gaattcgact ttgaaagcgc tgcccgcaag cagctaaaag gcaacatcta cctggccaag
     1741 gtgacgcggg tcgagccgtc a
//
LOCUS       DAORVN010000522         1761 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_76842_length_1761_cov_2.584408, whole genome shotgun sequence.
ACCESSION   DAORVN010000522 DAORVN010000000
VERSION     DAORVN010000522.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1761)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1761)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1761
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76842_length_1761_cov_2.584408"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            16..>1761
                     /locus_tag="PKV23_09790"
     CDS             16..>1761
                     /locus_tag="PKV23_09790"
                     /inference="COORDINATES: protein motif:HMM:NF014731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HQX85768.1"
                     /translation="MTQTSSPESEAALSSGPSSTKHKLAFASIGVVFGDIGTSPLYAM
                     REALHPVVSAGGDLRLAALGVVSLLLWALILVVGLKYLVFLMRADNRGEGGILSLVVL
                     VETLLKKKGGVVLALGIIGAAFFFGDAMITPAMSVLAAVEGLAVINPRFESVVVPLTL
                     AILFSLFLFQYRGTAGIASIFAPVTAVWFAAIGLLGLVHIGDDLQIFYAFNPLYGIQM
                     LVQQPGLALLVFGGVFLAVTGGEALYADMGHFGKKSIRLAWLCVVFPGLVLNYLGQGA
                     FIIAHPQHVENPFFLMMPTWGLIPLVILATLVTVIASQAVITGAFSIAQQAMSLGLLP
                     RMNITHTSETEQGQIYIAQINWMILLGVTLLVLVFRSSSNLASAYGIAVNTSMVVDTL
                     LALVFFWKARTLPLYIVIPALLGIFVIELTFLAANGLKLAKGGYVPVLFGATVILLMV
                     TWMRGRFALAAKLRRESIELVSLLQSLEKRQPTRVAGAAVFLQTDPIYAPSALMHNLK
                     HNRVLHDILVFISVETTDEPRADPDNRVSVKQLPLGAWLVEARFGYMEQPDVPAALRA
                     CEAQGLSIDPAQASYF"
BASE COUNT          295 a          562 c          471 g          433 t
ORIGIN      
        1 ccaaatgggc gtttcatgac ccagacaagc tctcccgaaa gtgaggcggc tctctcctca
       61 ggcccctctt caaccaagca caaattggcc ttcgcctcca ttggcgtcgt ctttggcgac
      121 atcggcacca gcccgcttta cgccatgcgc gaagccctac acccggttgt gagcgctggc
      181 ggcgaccttc gcctcgcagc gctcggcgtt gtctcactcc tgctctgggc tttaatcttg
      241 gtggtcgggc tgaagtatct cgtttttctc atgcgtgccg acaacagggg tgaaggcggc
      301 attctctcgc tcgtcgtgct ggtggaaaca cttttgaaga aaaagggcgg tgtggttctg
      361 gcccttggca tcatcggcgc cgccttcttc tttggtgatg cgatgattac ccctgccatg
      421 tcggtgctcg ccgccgtgga gggccttgcc gtcatcaatc cgcggtttga atctgttgtg
      481 gtcccactca ccctcgccat cctcttcagc ctgttcctgt tccagtatcg cggcaccgct
      541 ggcattgcct caatttttgc accggtgacc gcggtctggt ttgccgccat tggcctcttg
      601 ggcctagtcc atattggcga tgacctccaa atattctatg cctttaatcc gctctatggc
      661 attcaaatgc ttgtccagca gcctgggctg gcacttttgg tttttggcgg cgtgtttctg
      721 gcggtaaccg gcggtgaggc cctttacgcc gacatggggc atttcggaaa gaaatccatc
      781 cggctggcct ggctttgcgt ggtgttccct gggctggtgc tgaattacct cggccagggc
      841 gccttcatca ttgcccaccc gcaacacgtg gagaacccgt tcttcctgat gatgccgacc
      901 tggggcctta tcccgctcgt aatattggca acgctcgtga cggtcatcgc cagccaggca
      961 gtcatcaccg gcgccttttc cattgcccag caggccatgt ccctggggct gctgccgcgc
     1021 atgaacatca cccacaccag cgaaaccgaa caggggcaaa tctatatcgc ccagatcaac
     1081 tggatgatac ttttgggcgt aacactgctt gtgctggttt tccgttcctc atcgaacctc
     1141 gcctcagcct atggcatcgc ggtcaacaca tcgatggtgg ttgatacgct gctcgccctg
     1201 gtcttcttct ggaaggcccg gaccctcccg ctctatattg tcatacccgc cttgcttggc
     1261 attttcgtaa tcgaactgac cttccttgcc gccaatggat tgaagcttgc caagggcggc
     1321 tatgtgccgg tgctctttgg cgcaaccgtc atactgctta tggtcacctg gatgcgcggg
     1381 cgctttgccc tggccgccaa gctgcgccgg gaatcgattg agcttgtaag cctgttgcag
     1441 agcctggaga aacgccagcc cacccgcgtg gccggcgctg ccgtattcct gcagaccgat
     1501 ccaatttatg cgcccagcgc cctcatgcat aacttgaagc acaaccgcgt cctgcacgac
     1561 attctggtct tcatcagtgt tgaaacaacc gatgaaccgc gcgccgatcc ggacaaccgc
     1621 gtttcggtaa agcagctgcc tcttggcgcc tggctggtgg aagcccgctt tggctacatg
     1681 gaacagcccg acgtgcccgc cgccctgcgc gcctgcgaag cccaggggct ttcaatcgat
     1741 ccagcccagg cctcctattt c
//
LOCUS       DAORVN010000523         1758 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_77070_length_1758_cov_2.327070, whole genome shotgun sequence.
ACCESSION   DAORVN010000523 DAORVN010000000
VERSION     DAORVN010000523.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1758)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1758)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1758
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77070_length_1758_cov_2.327070"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..310)
                     /locus_tag="PKV23_09795"
     CDS             complement(<1..310)
                     /locus_tag="PKV23_09795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85769.1"
                     /translation="MKISKRYPQMVTSTAEEIREKTCELIDAMTAEAAAAGNPLTAEQ
                     TEEAESFKAAAFSNGEPRTGGFDPAPEEPDPFDPFSGGLPGLFGTGTTSPDRSIIVAT
                     V"
     gene            complement(539..988)
                     /locus_tag="PKV23_09800"
     CDS             complement(539..988)
                     /locus_tag="PKV23_09800"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HQX85770.1"
                     /translation="MPIFCPIYGLSDMGKAINFCYVHANIHCMTPIGLRLRALRKQLG
                     IGQAQLGALVGVNQSMISDYESKGVMFDADKLQALAEALNTTMDYIMYGDSDVDPDEA
                     ALIGAFRAMSPELRSALLKSATAFAAVPPKRPLLRVQKSDLGKRKAA"
     gene            987..1169
                     /locus_tag="PKV23_09805"
     CDS             987..1169
                     /locus_tag="PKV23_09805"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HQX85771.1"
                     /translation="MDWKTLIQKLIDMGMTQQQIAAHCGVSQSSISDLASGNAKKPRF
                     DTGAALVELAKRKEAA"
     gene            1166..1516
                     /locus_tag="PKV23_09810"
     CDS             1166..1516
                     /locus_tag="PKV23_09810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85772.1"
                     /translation="MRKPERFHQIPRFYSGCVYAAELTNGVVKVGFSRNPRTRMGTLV
                     LQARRMFGADVARFHIGLDMTEPRQALRAERELLTRVSKIGAPCKGSREFFSNLPFPV
                     AANLIDQLSRREYA"
     gene            1513..>1758
                     /locus_tag="PKV23_09815"
     CDS             1513..>1758
                     /locus_tag="PKV23_09815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85773.1"
                     /translation="MTPSTRTPIAVDAVARPAPEAADIVDIAGGYFAEKRADGFAIRH
                     ERGGLHMALPPEIAMVNAIAALRAELKELDALKVDIAN"
BASE COUNT          382 a          484 c          501 g          391 t
ORIGIN      
        1 agaccgtggc cacgatgatc gagcggtcgg ggctcgtcgt gccggtgccg aagagtccgg
       61 ggagcccgcc cgaaaacggg tcgaacggat caggttcctc aggtgccgga tcgaagccgc
      121 cggtccgcgg ttcgccattg gaaaatgcag cggccttgaa gctttcggct tcttcggttt
      181 gttcggcggt gagagggttc ccggcggcgg cggcctcggc tgtcatcgcg tcgatgagct
      241 cgcaggtttt ttcgcggatt tcctcggcgg ttgatgtgac catctgcggg taacgcttac
      301 taatcttcat ctcagctcct tggttagcag cgtgtttgct gcgatgggag aattatgggt
      361 tagccgatat gcaaagtaaa tgggttaagc cataatttgg cgcataatta ttttcatgcc
      421 attagattga ttgataggca atcactattt ggcaagcgca aaaaaacccg gtcatgccgg
      481 gcggttgatt acctgtttgt tgtgttgata acaaccaggc gcaatgtggc gtaggcgttt
      541 atgcggcctt gcgctttccc aagtctgatt tttgaaccct cagtaatggt cttttaggcg
      601 gcaccgcagc aaatgcggtc gcagatttaa gcaaagccga cctgagttcc ggcgacatgg
      661 cgcgaaacgc tcctataagc gcggcctcgt ccgggtccac atcgctgtcc ccatacatga
      721 tgtagtccat tgtggtgttc agcgcctcgg ccaaagcctg cagcttgtcg gcatcgaaca
      781 tcacaccctt agattcgtag tccgaaatca tcgactggtt gacgcccacc aaagcgccta
      841 gctgggcttg cccaatgcct agttgcttgc gtagcgccct caatcgaagt ccgatgggtg
      901 tcatgcaatg gatattcgca tgcacatagc aaaaattaat ggctttaccc atatcggata
      961 aaccataaat agggcaaaat atgggcatgg actggaaaac ccttattcaa aagcttatcg
     1021 acatggggat gacgcaacag cagatcgccg cacattgcgg cgtttcccag tcgtccatca
     1081 gcgacctggc gagcggcaac gccaagaaac cgcgcttcga taccggtgcg gcccttgtcg
     1141 agcttgcaaa gcgcaaggaa gcggcatgag aaagccagag cggttccacc aaatcccacg
     1201 gttctattcc ggctgcgtct atgcggcaga actcaccaac ggcgtcgtca aggtcgggtt
     1261 ctctcgaaat ccgcgcaccc gcatgggaac gctggtattg caagcccgca ggatgtttgg
     1321 cgctgacgtt gcccgttttc atatcggcct tgacatgacg gagccgagac aggccctgcg
     1381 ggcggagcgt gaattactga caagggtttc gaagatcggc gctccatgta aagggagccg
     1441 cgagtttttc agcaatctcc cgttccctgt tgcggcaaac ctgattgacc aactttcacg
     1501 cagggaatac gcatgacgcc aagtacacgc actccaatag ccgtcgatgc agtggcccgg
     1561 cctgcacccg aggcggcaga catcgttgat attgctggcg gctactttgc cgagaagcgc
     1621 gctgacggct ttgccattcg ccacgagcgc ggcggactgc acatggcgct tccgccagag
     1681 attgccatgg tgaacgccat cgccgccctg cgcgccgaac tgaaagagct tgacgcactc
     1741 aaggttgaca tcgccaat
//
LOCUS       DAORVN010000524         1755 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_77276_length_1755_cov_3.823529, whole genome shotgun sequence.
ACCESSION   DAORVN010000524 DAORVN010000000
VERSION     DAORVN010000524.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1755)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1755)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1755
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77276_length_1755_cov_3.823529"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            164..>1755
                     /locus_tag="PKV23_09820"
     CDS             164..>1755
                     /locus_tag="PKV23_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008191252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HQX85774.1"
                     /translation="MSIDKKASRKPSITDEEALEFHSMGKPGKIEINPTKPMATQRDL
                     SLAYSPGVAVPVRAIAENPDAAYDYTAKGNLVAVISNGTAILGLGDLGALASKPVMEG
                     KAVLFKRFADVDSIDLEVDTKDVDEFIGAVRYLGPSFGGINLEDIKAPECFMIEQRLR
                     ELMDIPVFHDDQHGTAIIATAALINALHITKRDIKDFKLVCNGAGAAAIACIELIKSM
                     GVPAGNVLLADSKGVVYQGRSEGMNQWKSAHATVTKARSLADAMEGADVFFGLSVAGA
                     LTPAMVKSMAKNPIIFAMANPDPEILPEEVAKIRSDAIMATGRSDYPNQVNNVLGFPY
                     IFRGALDVRARTINEEMKIAAAQALASLAREDVPDEVAAAYHGSRLAFGPQYLIPVPF
                     DPRLISRIPVAVAKAAMETGVARKHLADIDEYTAQLSARLDPIAGTLQTVYDQVRSKP
                     KRVVFAEGEEDRMIRAANTYATAGLGTAILIGREAEIEKTLSATGLELHEDVKILNAR
                     LTDRNVDYANFLYERLQRNGFL"
BASE COUNT          378 a          541 c          480 g          356 t
ORIGIN      
        1 gtcgcctgct ccagtcagtg tcgccatctc gttcatggcg gatttataac agatacctgg
       61 gcaagactgt caccccctat atttgctgaa tttagaggct tgagcattgc tggcactccc
      121 tctagtctcg ccccgcagcg ggatttgcat gaaatgagcg tcaatgagta ttgataagaa
      181 agccagccgg aagccatcca ttaccgatga agaagccctt gaatttcata gcatgggcaa
      241 gcccggcaag attgagatca atccgacaaa gcccatggca acccagcgcg atctgtccct
      301 ggcctatagt ccgggcgttg cggtgccggt acgcgccatc gctgaaaacc ctgatgccgc
      361 ctatgattat actgccaaag gcaacctcgt tgccgtcatc agcaatggca ccgccattct
      421 gggtcttggc gacctgggcg cccttgcttc gaaaccggtc atggaaggca aggccgtgct
      481 tttcaagcgc tttgccgatg ttgattccat tgatctcgag gttgatacca aggatgtcga
      541 tgagttcatt ggcgccgtgc gctatcttgg cccctctttt ggcgggataa atctcgagga
      601 catcaaggcc cccgaatgct tcatgatcga gcagcgcctg cgcgagctca tggacattcc
      661 cgtgttccat gatgaccagc acggaaccgc catcatagcc acagcggcgc tgatcaatgc
      721 gctccatatc accaagcgcg acatcaagga tttcaagctc gtctgcaatg gcgcgggcgc
      781 agcggcaatt gcctgcatcg aactgataaa atccatgggc gttccggcgg gaaacgtgct
      841 cctggccgat tccaagggcg tggtctatca gggccgctcc gaaggcatga accagtggaa
      901 atccgcccat gcaacggtca ctaaggcccg cagcctggcc gatgccatgg aaggtgccga
      961 tgtgtttttc ggcctctcgg ttgccggcgc gctcaccccg gccatggtca agagcatggc
     1021 caagaatccc atcatcttcg ccatggcaaa tcccgatccg gaaatcctgc cggaggaagt
     1081 ggccaagatc aggtccgatg ccatcatggc aaccgggcgc tccgattatc ccaaccaggt
     1141 gaacaatgtc ctgggtttcc cctacatatt caggggcgct ctcgatgtgc gggcccgcac
     1201 catcaatgag gaaatgaaga tcgccgccgc ccaggccctt gcaagcctgg cccgcgagga
     1261 cgttcccgac gaggtggccg ccgcctacca tggttcgcgg cttgcatttg gcccgcaata
     1321 cctgattccg gttccgttcg atccgcggct catttcgaga attccggtcg ccgtggccaa
     1381 ggctgccatg gaaacgggcg tggcccgcaa gcacctcgcc gatatcgatg agtacaccgc
     1441 gcaactttcc gccaggctcg acccaatcgc cggcaccctc cagaccgtgt atgaccaggt
     1501 aagatccaag cccaagcggg tggtcttcgc cgaaggcgaa gaggatcgga tgatccgcgc
     1561 cgcaaacact tatgcaacgg cgggtctggg gaccgccatc ctcattggcc gggaggccga
     1621 gattgaaaaa accctctccg caaccggcct cgaactgcat gaggacgtga agattctcaa
     1681 cgccaggctc accgaccgga atgtcgatta tgccaatttc ctctacgaac gcctgcagcg
     1741 caacggcttc ctctt
//
LOCUS       DAORVN010000525         1753 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_77445_length_1753_cov_3.402238, whole genome shotgun sequence.
ACCESSION   DAORVN010000525 DAORVN010000000
VERSION     DAORVN010000525.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1753
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77445_length_1753_cov_3.402238"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1142
                     /locus_tag="PKV23_09825"
     CDS             <1..1142
                     /locus_tag="PKV23_09825"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HQX85775.1"
                     /translation="LLGMNVCVSVGHNHPTVTQAVREQMEQLQHCTTMFYHPVPAHFA
                     EELAARMPKGHDWVVHFTNSGTEAVDLALVMAQQYTGNIDMLALRNAYHGATIGAQSL
                     TGISGFRHNIPLLTGIHHVANPDPYRGIFGDDTEAYLAEIDRTIQYATSGKLAGFVVE
                     PIQGYGGVVETPAGYLAAAAAKVRGRGGLMIIDEVQSGFGRTGDNFWGFEAHGMVPEI
                     VVMAKGIGNGFPLAAVVAQRHVADALAGKFSFHTYGASPTSCAAGRAVLQVIDEEGLQ
                     ANSKKVGALLRKVLDRLHQKHEIIGEVRSHGLMLAIELVTDRKSKSPAAAATADIFEK
                     TREHGLVMSKSGADKNILRMVPPMCIQENDVAQVEDALERVFSDY"
     gene            complement(1214..>1753)
                     /locus_tag="PKV23_09830"
     CDS             complement(1214..>1753)
                     /locus_tag="PKV23_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HQX85776.1"
                     /translation="PAPHDILAIKGVEELAAFLVNEIQEVYRLQGVSINDKHIEVIVR
                     QMLQKVEITDAGESTWLVGEQVDHVEMDTENDKLIADGRKPATGVPVLLGITKASLQT
                     RSFISAASFQETTRVLTEAAVQGKIDTLEGLKENVIVGRLIPAGTGGMLARLRQVADK
                     RDGLILEEQARAEVLPAAE"
BASE COUNT          365 a          565 c          486 g          337 t
ORIGIN      
        1 atctcctggg catgaacgtc tgcgtttcgg ttggccacaa ccaccctacg gtcacccaag
       61 ccgttcggga gcagatggag cagctccagc actgcaccac catgttctac catccggtgc
      121 ccgcccactt tgccgaagaa cttgcagccc gcatgcccaa aggccacgac tgggtggtgc
      181 acttcaccaa tagcggcacc gaggccgttg accttgccct ggtcatggcc cagcaataca
      241 ctggcaacat cgacatgctg gcactccgca atgcctatca tggagcaacc atcggcgccc
      301 agtcactcac cggcataagc ggcttccgcc acaacattcc cctcctcacc ggcattcacc
      361 acgtggccaa tccggaccct taccgaggca tattcggtga cgacacggag gcttacctcg
      421 cggaaattga ccgcaccatc caatacgcca catcgggcaa actcgcgggc ttcgttgtgg
      481 aacccatcca gggctatggc ggggttgtcg aaacacctgc tggctatctt gccgccgccg
      541 ccgcaaaggt gcgcgggcgg ggcgggctga tgatcatcga cgaggtccag tccggctttg
      601 gccgcaccgg cgacaatttc tggggcttcg aggcccatgg catggttccc gaaatcgtcg
      661 tcatggccaa aggaatcggc aatggcttcc cgctggcggc cgtagtggcc cagcgccatg
      721 tggccgacgc gctggcaggg aaattctcgt ttcacaccta tggcgcaagt ccaacctcct
      781 gcgccgccgg ccgagcggtc ctgcaggtca tcgatgaaga aggcctgcag gcaaactcca
      841 agaaagtcgg cgcccttttg cgtaaggtgc tggaccgcct tcaccagaag cacgaaatca
      901 ttggcgaagt gcgcagccac ggtctcatgc tggctatcga attggtgacg gaccgcaagt
      961 caaaatcccc ggcagcggcc gccaccgccg acatttttga aaagacacgt gaacacggcc
     1021 tggttatgag caaatccgga gctgacaaga acatcctgcg catggtgccg cccatgtgca
     1081 tccaggaaaa cgacgtggcc caggttgagg atgccctgga gcgtgtattc tccgactatt
     1141 gacgatactc caatagggca aacaaaaagg cccggcaata aaccgggcct tttcaatacg
     1201 atacaaagag cctttattcc gccgctggca gaacttccgc ccgcgcctgc tcctcgagga
     1261 tgaggccatc gcgcttgtcg gcaacctgac gcaaccgcgc cagcatgccg cccgtgcccg
     1321 ccggaatgag gcggccgacg atgacgtttt ccttgaggcc ttcgagcgta tcgatcttgc
     1381 cttggaccgc ggcttccgtg agaacccgcg ttgtttcctg gaaggacgcc gccgagatga
     1441 acgaccgtgt ctgcaaactc gccttggtga tgcccagcag aaccggaaca cccgtggcag
     1501 gcttgcggcc atcggcaatc agcttgtcgt tttccgtgtc catttcaaca tggtcgacct
     1561 gttcgccgac cagccaggtg gattcacccg catcggtgat ttcgaccttc tgcagcatct
     1621 ggcgcacgat cacttcgatg tgcttgtcgt tgatcgacac accctgcaaa cggtagactt
     1681 cctggatttc attgacgagg aaggcagcaa gctcttccac gcccttgatc gccagaatgt
     1741 cgtgcggcgc cgg
//
LOCUS       DAORVN010000526         1739 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_78596_length_1739_cov_2.826603, whole genome shotgun sequence.
ACCESSION   DAORVN010000526 DAORVN010000000
VERSION     DAORVN010000526.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1739
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78596_length_1739_cov_2.826603"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1114)
                     /locus_tag="PKV23_09835"
     CDS             complement(<1..1114)
                     /locus_tag="PKV23_09835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007199318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HQX85777.1"
                     /translation="MSDRSNSKPSIRTTIAQAGHFIDSQTGAVIPAIQPSTTFARKED
                     YSLVGNYIYSRNGSPTVAHAEGLLCELEESFETRLFASGMGAASALVETVESGQRIAA
                     PTIMYHGVKAWMLRQQAMRNVGIDLFDAADPASLARAIIPGKTAIVWIETPVNPTWDV
                     IDIRAAADAAHAAGAILVVDSTCAPPAISKPLTLGADIVFHSATKYLNGHSDVTAGVL
                     TAREASPRWEALKQAQIGLGATLGSFEAYLLIRGLRTLFLRVEQASRNALALARHFEK
                     HPKLAGVLYPGLESHPQHKIARKQMTGGFGGMLSILVKGDETQTRRVAGRLKVFTPAT
                     SLGGVESLAEHRKSVEGPHSIVAANLIRFSIGIEDVA"
     gene            complement(1107..>1739)
                     /locus_tag="PKV23_09840"
     CDS             complement(1107..>1739)
                     /locus_tag="PKV23_09840"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQX85778.1"
                     /translation="HPFPEDCPWDAGPEDHIQVDLSDSADTAAAILRMKDKLKEQGSK
                     LHALVNNAAISPKKPDGARLNTIETDEADWKKVFQVNFFAPVMLARGLIDELEAAKGA
                     VVNVTSIAGSRVHPFAGAAYSTSKAALASLTREMAADFGPIGVRVNSISPGEIETSIL
                     SPGTEKIIPQIPQRRLGQPEEVAKAIYFLCTDSSSYVTGAELHINGGQHV"
BASE COUNT          326 a          476 c          594 g          343 t
ORIGIN      
        1 cagccacgtc ttcgatgccg atggaaaagc ggatgaggtt tgcggccacg atgctgtgcg
       61 ggccttctac ggatttgcgg tgctcggcaa ggctttccac gccgcccagc gatgtggcgg
      121 gggtgaagac cttgaggcgg ccggcgacgc ggcgggtttg cgtctcgtcg cccttcacca
      181 aaatggaaag catgccgccg aaaccgccgg tcatctgctt cctggcgatt ttgtgctggg
      241 ggtggctttc caggccggga taaagaacac cagcaagctt gggatgcttt tcaaaatgcc
      301 tggcgagcgc aagggcattg cgcgaagcct gttcgacgcg gaggaaaagc gtgcggaggc
      361 cacggatcag aaggtaggct tcaaaactcc caagcgtggc gcccaggcca atttgcgcct
      421 gcttcagcgc ttcccagcgc gggctggctt cgcgggcggt caagacaccg gcggtcacgt
      481 cactatggcc gttgaggtat ttcgtagcgg aatggaaaac aatgtcagcg ccaagcgtga
      541 gcggcttgct gatcgctggc ggggcacagg tcgaatcaac gacgagaatt gcacccgccg
      601 catgggcggc atcggctgcg gccctgatgt cgatcacatc ccaggtggga ttcaccggcg
      661 tttcaatcca gacgatggcg gttttgccgg ggatgatggc gcgggccagg ctggcgggat
      721 cggcggcgtc aaacagatcg atgccgacat tgcgcatggc ctgctgacgc agcatccagg
      781 ccttcacccc gtgatacatg atagtggggg cagcgatgcg ctggccggat tctacagttt
      841 caacaagggc cgatgcggcc cccatgcccg atgcgaagag acgggtttca aagctctcct
      901 ccagttcgca gagcaagcct tcggcatggg ccacggtggg cgaaccgttg cgcgaataga
      961 tgtagttccc cacaaggctg taatcctcct tgcgggcaaa ggtggtggac ggctggatgg
     1021 cggggattac cgcgccagtc tggctgtcga tgaagtggcc ggcctgggcg atggtggtgc
     1081 ggatggacgg tttcgaattc gagcggtcag acatgctggc cgccgttgat atggagttcg
     1141 gcgccggtca cgtagctgga actgtccgta cacagaaaat atatggcctt ggccacttcc
     1201 tccggctggc caaggcggcg ctggggaatc tgcggaatga tcttctcggt gccgggcgaa
     1261 aggatcgagg tttcgatttc accgggcgag atggagttta cccgcacgcc gatgggaccg
     1321 aagtcggcgg ccatttcacg ggtgagcgag gcgagcgccg ccttcgaggt ggagtaagcg
     1381 gcgccggcaa agggatggac gcgcgagccg gcgatggagg tgacgttcac caccgcaccc
     1441 ttggcggcct ccaactcgtc gataaggccg cgggccagca tcacgggggc gaagaaattc
     1501 acctggaaca ctttcttcca gtctgcttca tcggtttcga tcgtgttcag ccgtgcgcca
     1561 tcgggtttct tggggctgat ggcggcgttg ttgaccaggg catgaagctt cgagccctgt
     1621 tctttcagtt tatctttcat gcggagaatt gcggcggcgg tgtcagcgga atcgctgaga
     1681 tcaacctgga tgtgatcttc cgggcccgca tcccacggac aatcctcggg gaagggatg
//
LOCUS       DAORVN010000527         1738 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_78667_length_1738_cov_3.220440, whole genome shotgun sequence.
ACCESSION   DAORVN010000527 DAORVN010000000
VERSION     DAORVN010000527.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1738
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78667_length_1738_cov_3.220440"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(252..497)
                     /locus_tag="PKV23_09845"
     CDS             complement(252..497)
                     /locus_tag="PKV23_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015316743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HQX85779.1"
                     /translation="MAEVHLWGALRPIVGGALSVSVEAKNIRELFKVLAEQYPAFEPH
                     LQRGIAVSINGKIYRDTWDQALPPDAEIYLLPRIPGG"
     gene            complement(502..>1738)
                     /locus_tag="PKV23_09850"
     CDS             complement(502..>1738)
                     /locus_tag="PKV23_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006338839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HQX85780.1"
                     /translation="AFACYDLKNVKSESIEVIVNRPKTAAYRAPSAPMAAFAVESAVD
                     ELAKKIGMDPVELRIKNAAKQGTQSSYGPVYGPIGIGATLEAAKNHPHMKAPLGKNQG
                     RGMACGFWFNFGGQTCADLNVTPDGTVTLAVGTIDVGGSRASIGLMAAEELGIPYENV
                     RVVVADTSSLGHNDMTDGSRGTFSTGLATIAAARNAIKVLCARAAKMWDIPEDAVIWE
                     DGHAKPAGTNAGNFPPLSIKEIAAAAGNTGGPIAGHNELVADGAGVSFATHICDIEVD
                     PETGRSTVKRYTVIQDAGKAIHPSYVEGQYQGGAAQGIGWALNEEYIYGKDGRLQNAG
                     FLDYRIPVCSDLPFIDTMILEIPNPNHPYGVRGVGETSIVPPLAAIANAMSNAVGVRM
                     THIPMSPPRVLKAISNRKH"
BASE COUNT          290 a          501 c          590 g          357 t
ORIGIN      
        1 caggcgggcg gttcatctcg tcgatgaact gctcgacgaa tgccagtggc tggcccatcg
       61 cgccgcgatg cccgacacga gttgatcatc cgtatacgga ttaaaatatt aaccacccta
      121 cttctgtcat cccggcgaac gccgggaccc agacttgacg cgtgtgtgat tgttgaaagt
      181 ttgggccccg actttcgccg gggtgacaga actttggaag ctttcaccgg aatgacaaat
      241 gattggattt ttcatccccc cggaatgcgc gggaggagat agatttccgc gtcgggcggc
      301 aaggcctggt cccaggtgtc gcggtagatc ttgccattga tggagacggc gatgccacgc
      361 tgaagatgcg gctcgaaggc ggggtattgc tcggccagaa ccttgaacag ctcgcggatg
      421 tttttcgcct cgactgaaac ggaaagcgct ccgccaacga ttggccggag cgctccccag
      481 aggtggactt ccgccacgtt gctagtgctt gcggttgctg atcgccttga gcacgcgggg
      541 cggcgacatc gggatatgcg tcatgcggac gccgacggcg ttcgacatgg cgttggcaat
      601 ggcagccagc ggcggcacga tggaagtttc gccaacgccg cgcacgccat aggggtggtt
      661 ggggttggga atttccagga tcatcgtgtc gatgaagggc aggtccgagc agaccgggat
      721 gcggtagtcg aggaagcccg cgttctgcag gcggccatcc ttgccgtaga tatattcctc
      781 gttcagcgcc cagccgatgc cctgggcggc gccgccctgg tactggcctt ccacatagga
      841 gggatgaatg gctttgcccg catcctgaat cacggtgtag cgcttgacgg tggagcggcc
      901 ggtttccggg tcgacctcga tatcgcagat atgcgtggcg aaggatacgc cggcgccatc
      961 ggccaccagt tcattgtggc cggcgatcgg cccgccggtg ttgccggcgg ccgccgcgat
     1021 ttccttgatg gagagcggcg ggaaattgcc ggcgttggtg ccggctggct tggcatggcc
     1081 atcttcccag atcacggcgt cttccggaat gtcccacatc tttgcggcgc gggcgcagag
     1141 caccttgatg gcgttgcgcg ctgcggcgat ggtggcaagg ccggttgaga aggtgccgcg
     1201 gctgccgtcg gtcatgtcgt tgtggccgag cgacgatgtg tcggcaacca ccacgcgcac
     1261 gttctcgtag gggatgccga gctcttccgc cgccatgagg ccgatggaag cgcgcgagcc
     1321 gcccacgtcg atggtgccca cggcgagagt taccgtgcca tcgggcgtga cgttcaggtc
     1381 ggcgcaggtc tgtccgccga agttgaacca gaagccgcag gccatgccgc gtccctggtt
     1441 cttgcccagc ggcgccttca tgtgcggatg gttcttggcg gcttcgagtg tggcgccgat
     1501 gccgattggg ccatagaccg ggccatagga gctttgggtg ccctgcttgg cggcattctt
     1561 gatgcggagc tcgaccggat ccatgccgat cttctttgcc agctcatcaa cggcgctttc
     1621 cacggcgaag gctgccatgg gagccgacgg ggcgcggtag gcggccgtct tcgggcggtt
     1681 gacgatgacc tcgatgcttt cgctctttac gttcttgaga tcgtaacagg caaaggcc
//
LOCUS       DAORVN010000528         1737 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_78779_length_1737_cov_2.177765, whole genome shotgun sequence.
ACCESSION   DAORVN010000528 DAORVN010000000
VERSION     DAORVN010000528.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1737
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78779_length_1737_cov_2.177765"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..814
                     /gene="hslU"
                     /locus_tag="PKV23_09855"
     CDS             <1..814
                     /gene="hslU"
                     /locus_tag="PKV23_09855"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008548289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HQX85781.1"
                     /translation="IQVADTAGAAFDIPGMPGGSASVINISEIMGKAFGGRTKTRRMT
                     VKASYELLIAEESDKLLDPEQINQEAIDLVENNGIVFLDEIDKICARAERQGGDVSRE
                     GVQRDLLPLIEGTTVSTKYGPVKTDHVLFIASGAFHIAKPSDLLPELQGRLPIRVELK
                     ALGREDFRRILTEPECSLIKQYKALLGTEGIELNFTEDSIAAIANIAAEINASIENIG
                     ARRLHTVLERVLDDISYTATDRSGEAVMIDEAYVQKHVGDLAKNADLSKFIL"
     gene            976..1722
                     /locus_tag="PKV23_09860"
     CDS             976..1722
                     /locus_tag="PKV23_09860"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HQX85782.1"
                     /translation="MFCFAAMIAFHQIGPALAADLIDAVRAGNVARVGILLDSGADPN
                     KRSSYNGPLHDAARIGSAEITTILIKAGADVELSGFGGIRPLHSAALAGQAKVVSILL
                     ANGAKVDSRDNTGRTPLLSFMSGHTADVSTLIALLEAGADPNLLDGPVNYHPLDYAAM
                     HGRADVAELLIAFGADMNAKDNLYGGTPLHYAIWYAPRGHLEVAQLLIDRGADVNAKD
                     VNGLTPLDNAKLYAPNAGLLQMLLTQAGAR"
BASE COUNT          369 a          501 c          525 g          342 t
ORIGIN      
        1 aatccaggtg gccgacacgg ccggtgccgc cttcgacatt ccggggatgc cggggggttc
       61 ggccagcgtg atcaacatct ccgagatcat gggcaaggcc tttggcgggc gcaccaagac
      121 gcggcgcatg acggtgaagg cgagctacga acttctgatc gccgaggaaa gcgacaagct
      181 gctggacccg gagcagatca accaggaagc catcgacctt gtcgagaaca acggcatcgt
      241 cttccttgac gagatcgaca agatctgtgc ccgggccgag cgccagggcg gcgatgtgag
      301 ccgcgagggc gtgcagcgcg acctgctgcc gctcattgaa ggcaccacgg tttcaacgaa
      361 atacggcccg gtgaaaaccg accacgtgct gttcatcgcc tcgggcgcct tccacatcgc
      421 caagccttcc gatctgttgc cggaattgca gggccgtttg ccgatccggg ttgaactcaa
      481 ggcgctgggc cgggaagatt tccgccggat cctcaccgag ccggaatgca gcctgatcaa
      541 gcaatacaag gcgctgctgg gaaccgaggg aattgagcta aatttcaccg aggacagcat
      601 tgcggcaatc gcaaatatcg ccgccgagat caatgcctcc atcgaaaaca tcggagcgag
      661 gcggctgcac acggtgctgg aacgggtgct cgacgacatc agctacacgg caaccgaccg
      721 ctccggcgaa gcggtgatga tcgacgaggc ctatgtgcaa aagcatgtgg gcgatctggc
      781 gaagaacgcg gacttgagca agtttatttt gtaggagcaa accctccgcc ggctgcaaca
      841 agccaagaaa gctccgggcg cagatggact tgtatctgaa gcccgatcct gcgaagctcc
      901 ccttcccaac tcgtgatcgc gtgcgactaa gatttggagc gacttgcaat ggaattgcgg
      961 cggtcgaatt tcccggtgtt ctgcttcgcg gcgatgattg ctttccatca gattggacca
     1021 gctcttgccg ccgatttgat tgatgccgtc agagccggaa acgtggcccg tgttgggatc
     1081 ttgctggatt ccggcgcaga tccaaacaag cggtcttctt acaatggtcc cctgcacgac
     1141 gcggctcgga taggttcggc tgaaataacc accattctca ttaaggctgg ggcagacgtg
     1201 gagctttccg gtttcggggg tattcgtccc cttcattcag ctgccttggc gggtcaggca
     1261 aaggtcgtgt ccatacttct tgcgaatggc gcaaaggtgg attcccgcga caacaccggg
     1321 cggaccccgc ttctttcgtt catgtccggt cataccgctg acgtcagcac cctcattgcc
     1381 ctgttggagg ccggagccga tccaaatctc ctcgacggac cagtgaacta ccacccgctg
     1441 gactatgctg caatgcatgg tcgtgcggat gtggccgaac ttttgattgc ctttggcgcg
     1501 gacatgaatg caaaggacaa cctctatggc gggacgcccc tgcactatgc aatatggtat
     1561 gcgccgcggg gccatctgga agtggcgcag ctcttgatag accgcggcgc cgatgtgaat
     1621 gccaaggacg ttaatggttt aacgccgctg gacaatgcca agctctatgc tccgaacgcc
     1681 ggcctgctgc aaatgcttct gacccaggcg ggcgcgcgat agaggttggg cactaca
//
LOCUS       DAORVN010000529         1735 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_78903_length_1735_cov_3.255952, whole genome shotgun sequence.
ACCESSION   DAORVN010000529 DAORVN010000000
VERSION     DAORVN010000529.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1735
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78903_length_1735_cov_3.255952"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..326)
                     /locus_tag="PKV23_09865"
     CDS             complement(<1..326)
                     /locus_tag="PKV23_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009757793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HQX85783.1"
                     /translation="MNPAKITCIEDLRQIYESRVPRMFYDYCDTGSYTTGTYVDNTRA
                     FDKYKLRQRVARNIDNRSLKTRMVGQDVSMPVALAPIGLGGMQHADGEIKACRAAQKF
                     GVPYTLT"
     gene            439..>1735
                     /locus_tag="PKV23_09870"
     CDS             439..>1735
                     /locus_tag="PKV23_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008509458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HQX85784.1"
                     /translation="MLKMPEADAQTVKKRASIAAALRRIVPGEGVIETRTEMSVYESD
                     GLTAYRQLPLVVVLPNSTAEVAKVLRYAFENKIKVVPRGAGTSLSGGALPLADGIVLG
                     LSKFNRVLEIDYDNRCAVVQPGVTNLAITQAVQHKGFYYAPDPSSQIACTIGGNVAEN
                     SGGVHCLKYGLTANNILGLEMVLITGEVIRLGGKHLDSDGLDLLGVLTGSEGLLGVIT
                     EVTVRLLQKPETARVILVGFPTNEDAGKCVADIISAGIIPGGMEMMDRPAIHAVEEFV
                     HADYPLDVEALLIIELDGPAIEVDHLIDEVSVIAKKNKCSTSRTSTSEAERLLFWAGR
                     KAAFPAVGRISPDYFCMDGTIPRKRLSEVLTGMGELSKKHGLRVANVFHAGDGNLHPL
                     ILYDANVADELARAEEFGADILRLCVKVGGVLTGEHGVGI"
BASE COUNT          322 a          505 c          554 g          354 t
ORIGIN      
        1 gtgagcgtat agggcacgcc gaatttctgc gcggcgcggc aagccttgat ttcaccgtcg
       61 gcgtgttgca tgccgccgag cccgatgggc gccagggcca ccggcatgga gacatcctga
      121 cccaccatcc tggtcttcag cgagcggttg tcgatgttgc gggcaacgcg ctggcggagc
      181 ttgtatttat cgaaggctct ggtgttatcg acataggtgc ccgtcgtgta ggaccccgtg
      241 tcgcaatagt cgtaaaacat ccggggcacg cgggattcgt agatctggcg caaatcttca
      301 atacaggtga ttttggcggg attcatctcg ggttgcttct caatatgggg cgtgcgttcc
      361 ctcttttgcc cgccttgtcg gtccactgca aggtagtttc tcttgttaaa tcatcccatt
      421 gcggggtagt ttcgggccat gctgaaaatg cccgaagcag acgcccagac agtcaagaag
      481 cgcgcctcga ttgcggctgc gctccgccgc atcgtgccgg gggaaggggt gatcgagacg
      541 cgcactgaaa tgagcgttta cgagtccgac gggctcaccg cctaccggca gttgccgctc
      601 gtggtggtgc tgcccaacag caccgcggaa gtggcaaaag tgctgcgcta cgccttcgaa
      661 aacaaaatca aggtcgtgcc acggggtgcg ggtacctcgc tatccggcgg agccctgccg
      721 ctggccgacg gcattgttct tgggctttcg aaattcaacc gggtgctgga aatcgactat
      781 gacaaccgct gcgccgtggt gcagccgggg gtcaccaacc tggccatcac ccaggcggtg
      841 cagcacaagg gtttctacta tgcgcccgac ccttcctcgc agatcgcctg caccattggc
      901 ggcaatgtgg cggaaaattc cggcggggtg cactgcctga aatacggcct caccgccaac
      961 aatatcctgg gcctggaaat ggtgctgata accggcgagg tcatccggct cggcggcaag
     1021 cacctggaca gcgacgggct ggacctcctc ggcgtgctca ccggttcgga gggcctcctc
     1081 ggggtaatca ccgaggttac agtgcggctc ctgcagaagc cggaaacggc ccgtgtcatc
     1141 ctggtgggct ttccgacaaa cgaggatgcg ggcaagtgcg tggccgatat catttccgcc
     1201 ggcatcattc ccggcggcat ggaaatgatg gacaggcctg ccattcacgc agtggaagaa
     1261 tttgtccatg cggactatcc gcttgatgtg gaggcgctgc tcatcatcga acttgacggg
     1321 ccggccatcg aggtggacca cctgattgac gaggtttcgg tgattgccaa gaagaataag
     1381 tgcagcacct cgcgcacctc aacttcggaa gccgagcgcc tgctgttctg ggccgggcgc
     1441 aaggcggcgt tcccggcggt ggggcgcatc tcgccggatt atttctgcat ggatggcacc
     1501 attccgcgca agcgcctctc ggaagttctc accggcatgg gtgagctttc aaaaaagcac
     1561 gggctgcggg tggccaatgt tttccatgcg ggtgacggca atctgcatcc gctcattctc
     1621 tacgacgcca atgtggctga tgaactggcg cgggccgagg aattcggcgc cgacatcctg
     1681 cggctctgcg tcaaggtggg cggcgtgctc accggcgagc atggcgtcgg catcg
//
LOCUS       DAORVN010000530         1735 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_78923_length_1735_cov_2.451190, whole genome shotgun sequence.
ACCESSION   DAORVN010000530 DAORVN010000000
VERSION     DAORVN010000530.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1735
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78923_length_1735_cov_2.451190"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..395)
                     /locus_tag="PKV23_09875"
     CDS             complement(<1..395)
                     /locus_tag="PKV23_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HQX85785.1"
                     /translation="MSRIAIFVGHARKDTYCEALGEAYRRGAEAGGHQATLFVTSKMV
                     FDPILHEGFAKIQPLEPDLQAAHEAIIAADHLVFIFPLWLGTLPAIFKGFLERVLQPD
                     LVEPSKKGKYVKFLKGKSARVIMTMGMPG"
     gene            complement(411..797)
                     /locus_tag="PKV23_09880"
     CDS             complement(411..797)
                     /locus_tag="PKV23_09880"
                     /inference="COORDINATES: protein motif:HMM:NF013331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II truncated hemoglobin"
                     /protein_id="HQX85786.1"
                     /translation="MRKSPYQMLGGEAAVERLVQEFYDIVESDPAAASLLAMHNRGHG
                     LAHARQVQFEFLSGFLGGPQLYFERHRHSNVRQMHAHLAIGTAEKDAWLACMDKALAA
                     VVAESETRRLLMGHFSRVAEALRNQA"
     gene            932..>1735
                     /locus_tag="PKV23_09885"
     CDS             932..>1735
                     /locus_tag="PKV23_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003525001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HQX85787.1"
                     /translation="MNGYGVLAKLISLGVVALVAVIAAAKGHDAAYVFHAYVISAVAM
                     GLIVYYSRSFSFAPVGPAPATESTYSDEVIKAGMIATVFWGVAGFLLGVVIASQLAWP
                     ALNLDLPWTTFGRLRPLHTSAVVFAFGGNALIATSFYVVQRTCRTRLAFGNLAWFVFW
                     GYQLFIVLAATGYLLGVTESREYAEPEWYVDIWITLVWVAYFLVFFGTLLKRKEPHIY
                     VANWFYLAFIVTIAMLHIVNNLAVPVSFLGSKSYSLFSGVQDALIQWWYG"
BASE COUNT          319 a          520 c          519 g          377 t
ORIGIN      
        1 aagccgggca tgcccatggt catgatgacg cgggctgatt tgcctttgag aaattttacg
       61 tactttccct ttttggaagg ctccaccagg tcaggctgca ggacgcgctc gagaaatccc
      121 ttgaagatgg cgggaagggt tccgagccag agcggaaaaa tgaagacaag atgatcggcc
      181 gcgatgatcg cctcgtgcgc cgcctgcagg tcgggctcga gcggctgaat ctttgcaaag
      241 ccttcgtgca ggatgggatc aaacaccatc ttcgaggtca cgaacagggt ggcctgatgc
      301 ccgccggctt cggcgccgcg ccggtaggct tcaccaaggg cttcacaata cgtgtccttc
      361 cttgcgtgac cgacaaagat tgcaatccgc gacatccgca ctcccttgat tcaggcctga
      421 ttgcggagcg cttcggcgac gcggctgaaa tgccccatga gcaggcggcg ggtttcagac
      481 tcggcaacca cggcggccag ggccttgtcc atgcaggcga gccatgcatc cttttcggcc
      541 gttccgattg caagatgggc gtgcatctgc cggacattgg agtggcggtg gcgttcgaaa
      601 tagagctgcg ggccgccaag aaagcccgag agaaactcga actgcacctg gcgcgcatgg
      661 gcaaggccat gaccccggtt atgcatggcc aggagagatg cagcggcagg atcgctttcg
      721 acaatatcat agaattcctg caccagccgc tcgacggcgg cctcgccgcc cagcatctga
      781 tagggtgact tccgcatcgg cacgcccttc caaatctatt ttgcgaatca cgatacaagc
      841 tccaaactcc cgtcaattga tctggatcaa tgaattgcga tgcctgatcg ggcaacaacc
      901 ggttggtctg aatatgaaag gcggggagcc aatgaacggt tatggagtgc tggcgaagct
      961 gatttcgctg ggcgttgtcg cccttgtggc tgtcatcgct gccgccaaag ggcatgacgc
     1021 ggcctatgtc ttccacgcct atgtgatttc ggcggtggcc atgggcctca tcgtctacta
     1081 ttcgcggagc ttctcctttg cccccgtggg gcccgcgccg gcaacggaaa gcacctattc
     1141 ggatgaggtc atcaaggccg gcatgatcgc cacagtcttc tggggcgtgg cgggattcct
     1201 cctgggcgtg gtcatcgcct cgcagcttgc ctggccggcg ctcaatctcg acctgccatg
     1261 gacgacattc ggccgcctgc gcccgctgca cacctcggcg gtggttttcg cctttggcgg
     1321 aaacgcactg atcgccacca gtttctatgt tgtccagcgc acctgccgca cccggctggc
     1381 cttcggcaat ctcgcctggt tcgtgttctg gggctaccag ctgttcatcg ttctggcggc
     1441 caccggctat ctcctggggg tgaccgagtc gcgcgaatat gccgagcccg aatggtatgt
     1501 cgatatctgg atcacccttg tctgggtcgc ctatttcctg gtgttcttcg gcaccctgct
     1561 gaagcgcaag gagccccaca tttacgtggc gaactggttc tacctggcgt tcatcgtcac
     1621 gatcgcgatg ctgcacatcg tcaacaacct ggcggtaccg gtctcattcc tcggctcgaa
     1681 gagctattcg ctgttctccg gcgtccagga cgcgctgatc cagtggtggt acggc
//
LOCUS       DAORVN010000531         1733 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_79084_length_1733_cov_2.269368, whole genome shotgun sequence.
ACCESSION   DAORVN010000531 DAORVN010000000
VERSION     DAORVN010000531.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1733)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1733)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1733
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79084_length_1733_cov_2.269368"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            93..377
                     /locus_tag="PKV23_09890"
     CDS             93..377
                     /locus_tag="PKV23_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007008263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85788.1"
                     /translation="MPKINFAGYKVNVPGHPLLRVGFGVLLVIGGLLGFLPIVGFWMV
                     PLGLLVLSVDFPPVRRFRRNATVKIGYWLHKRWPGLAKILGFGAPRDEKL"
     gene            complement(383..456)
                     /locus_tag="PKV23_09895"
     tRNA            complement(383..456)
                     /locus_tag="PKV23_09895"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(422..424),aa:Cys,seq:gca)
     gene            602..1252
                     /locus_tag="PKV23_09900"
     CDS             602..1252
                     /locus_tag="PKV23_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012111931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HQX85789.1"
                     /translation="MTDFAVARHNMVESQVRPNGITDHRIIDAMAQVKREDFVPEGRK
                     AIAYLDEDVELKNGRYLIEPMAFARMIHLALIKPTDRVLVVGAGTGYGARVISLLAKS
                     VVALESDPELAGLARGFLAGDAGVEVVEGSLAEGYPAGAPYDVIIVEGRIAAVPQGLF
                     DQLANEGRIVAAVGNTDVSKMQIATLADGHRSSRAAFDVSIAALPGFAAEKAAFVF"
     gene            1409..>1733
                     /locus_tag="PKV23_09905"
     CDS             1409..>1733
                     /locus_tag="PKV23_09905"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HQX85790.1"
                     /translation="MLTSAALVVAAGQVSAETLVEAMVSAYSSNPTLHAERARQRGTD
                     ELVPQALSGWRPTVNTEGSIANVWSDASGAPATENDPKSVAIGLSQPVFRGFKTIMGT
                     KAAEAN"
BASE COUNT          373 a          446 c          545 g          369 t
ORIGIN      
        1 gactaaaaga cattttgttg ttactttgac cgcccactgt gcatagggcg atcacgccct
       61 atataaggtc ctcttctgaa gaaaggatta tcgtgccaaa aatcaatttc gcgggttaca
      121 aggtgaacgt tccgggccat ccgctgctgc gcgtgggctt cggcgtgctc cttgtcatcg
      181 gcgggcttct gggattcctg ccgattgtcg gcttctggat ggttcccctg gggctgcttg
      241 tgctttcggt cgatttcccc ccggtgcgcc ggttcaggcg caatgccacg gtcaagatcg
      301 gctactggct gcacaagcgc tggcccggcc ttgccaaaat attgggcttt ggcgcgccgc
      361 gtgatgaaaa gctctagatt tctggaggcc tcgcccagaa tcgaactggg gtgcaaggat
      421 ttgcagtcct ctgcgtaacc actccgccac gaggccttcg ggcggctgta taggggatga
      481 aaattgagga aacaactgaa acataaaaca taataaatca cagcaacagt ttgcttggca
      541 aaactggcgg gcgcggctag tttggcgggc acctgacaaa acaagcgaaa cggacccata
      601 aatgacggat tttgcagtag cgcggcacaa tatggtcgaa tcccaggttc gccccaacgg
      661 catcaccgat caccgtatca tcgatgccat ggcgcaggtg aaacgcgagg acttcgtgcc
      721 ggaggggcgc aaggccatag cctatcttga cgaagatgta gagttgaaga acggacgata
      781 cctgatcgaa cccatggcct ttgcccggat gattcatctc gccctgatca agcccacgga
      841 ccgggttctg gtggtggggg cgggcacggg ttatggagcc cgggtcattt cactgctggc
      901 aaagtcagtg gtggccctgg agtcggatcc ggagcttgca gggttggccc gcggcttcct
      961 agccggcgac gcgggggtcg aggtcgttga aggcagcctg gcggaaggct acccggcggg
     1021 agcgccttat gacgtcataa tcgtcgaggg ccggattgcc gccgtgccgc aaggcctgtt
     1081 tgaccagctg gccaatgaag ggcgcattgt ggcggcggtc ggtaacacgg atgtttccaa
     1141 gatgcagatt gcgacgctgg cggatgggca ccgttcgtca cgtgcggcct ttgatgtgtc
     1201 aattgcggcg cttccgggat ttgccgctga aaaagcagca tttgtttttt gaatgaaaag
     1261 gttgcaaaaa tacacttcga ttcggtcggc gaataatcta ggtttgaagt tcgggtaaag
     1321 agccacggat ttcctgtggc ctgggatgac tgaaactcga aaagcgtggt gtggaatgca
     1381 aggtggtttg aagcattgga cgagaatatt gctgacctca gccgcacttg tggtggcggc
     1441 ggggcaggtc tcggctgaaa ccctggttga ggccatggtc tcagcctatt cgagcaatcc
     1501 gacactgcat gccgaacgcg caaggcaaag gggaacggac gaactggttc cgcaggccct
     1561 gtcgggctgg cggcccaccg tcaatactga aggatcaatt gccaacgtct ggtccgacgc
     1621 aagcggcgcg ccagcaacgg aaaatgatcc gaagtcggtg gccattggcc tgtcgcagcc
     1681 ggttttccgg ggcttcaaga ccatcatggg aaccaaagcg gctgaagcca atg
//
LOCUS       DAORVN010000532         1731 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_79224_length_1731_cov_2.755370, whole genome shotgun sequence.
ACCESSION   DAORVN010000532 DAORVN010000000
VERSION     DAORVN010000532.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79224_length_1731_cov_2.755370"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..291)
                     /locus_tag="PKV23_09910"
     CDS             complement(<1..291)
                     /locus_tag="PKV23_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018426501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85791.1"
                     /translation="MRAALSKYEDPYVAVRDLYLSTVGCVHYDGMKMEGHMHYPKGAM
                     GIHFVNLTIQGPPDPLKPNVLIYEPVGDKLKLVAVEWPVPLPAAIERPVLLGQ"
     gene            complement(520..849)
                     /locus_tag="PKV23_09915"
     CDS             complement(520..849)
                     /locus_tag="PKV23_09915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85792.1"
                     /translation="MKKIIFASIFALGLAMGFPAVAQADSSGKDYFDRREIGDPFGCY
                     GCYPRYGIYQGKMSCSAAKARVRNSGYRNVETIECRGITYTFEATRKGRDVIVLVNSR
                     TGAVWRR"
     gene            complement(985..1524)
                     /locus_tag="PKV23_09920"
     CDS             complement(985..1524)
                     /locus_tag="PKV23_09920"
                     /inference="COORDINATES: protein motif:HMM:NF013003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HQX85793.1"
                     /translation="MFQNLGAAIVALTTVVMAAPTLAGDAGQLQQLLSTRKCPGCDLS
                     NANLGSANLWNADLRHANLADADLRGTNLKYANLRGAILIDADLRGADMFSANLGGDA
                     DLSGADLGGANLHGAKLSGVSLQGAKLNGTNLTIADLWEANLKGADLTGAILNKTDLS
                     RAIWTDGRICADSSVGECK"
     gene            complement(1614..1721)
                     /locus_tag="PKV23_09925"
     CDS             complement(1614..1721)
                     /locus_tag="PKV23_09925"
                     /inference="COORDINATES: protein motif:HMM:NF016274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004668 - protein-arginine deiminase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HQX85794.1"
                     /translation="MFKDLEIVPVPSRALLYAGGNIHCITQQEPVTGPA"
BASE COUNT          344 a          517 c          517 g          353 t
ORIGIN      
        1 ttggccgagg agcaccggtc gctcgatggc cgccggcagc ggcaccggcc attccactgc
       61 cacaagcttc agcttgtcgc ccaccggctc atagatcagc acattgggct tcaggggatc
      121 gggtggcccc tggatggtga ggttgacgaa gtgaatgccc atggcgccct tggggtaatg
      181 catgtggcct tccatcttca tgccgtcata atgcacgcag cctaccgtcg acaggtaaag
      241 gtcgcgcacg gcgacgtaag ggtcttcata tttggaaagt gcggcgcgca tggcgtcgat
      301 ctggtcttgc ggcacgtcct cggcaaaggc ggtgccggcg catgtgcaaa cgccgagcgc
      361 taccgcggcg gcgagcccga aacggaactt ggtcatcctg gatcctcccg acagtctgca
      421 accggcgtct tcaaacggac atcgtattgc aggcttcagc ttttcacaga aaacgcctga
      481 ttggcaagac agataaaagg tcaggattgc tgctgcaatc tagcggcgcc agacggcacc
      541 ggtccgggag ttcaccagga caatgacatc gcggcccttg cgcgtggcct cgaaggtgta
      601 ggtgatgccg cggcattcga tggtctcgac attgcggtag ccgctgttgc ggacgcgagc
      661 cttggcggcg ctgcaggaca ttttgccctg gtaaatgccg tagcgcggat agcagccgta
      721 gcagccaaag ggatcgccga tttcgcgccg gtcgaaatag tccttcccgc tggaatcggc
      781 ctgggcgacg gcgggaaaac ccatggccaa gcccagggca aagatcgagg cgaaaatgat
      841 tttcttcatg gcaaactcct tgcctggttt acgccggtgg cggaagaacg gattacagcc
      901 cgcgaaagtt ccggaggatc atgtccatca tatgggcatt tccatggcgg gtgcacaaga
      961 acactaagcg acgattcaca accactattt gcattcaccg acagaactgt cggcgcatat
     1021 cctgccgtct gtccagattg ccctgctcaa gtccgtcttg ttcaagatag caccggtcaa
     1081 gtccgcacct ttcaggttcg cctcccacag gtcggcgatg gtcaagttgg tgccgttcaa
     1141 ctttgcgccc tgcaggctaa caccgctcaa ttttgcgcca tgcaggttcg ctccacccaa
     1201 atctgcgccg ctgaggtccg cgtcgccgcc caaattcgct gagaacatat cagctccgcg
     1261 caagtccgca tcaatcagta tcgcaccgcg caggttggca tatttcaggt ttgtcccacg
     1321 caagtccgcg tcggccaagt tggcgtgacg cagatcggcg ttccacaggt tggcgctgcc
     1381 aaggttcgcg ttgctcaaat cgcaccccgg gcatttcctg gtgctgagga gttgttgaag
     1441 ctgcccggca tcgcccgcca gcgtaggagc ggccatgacc accgtggtca gcgcgacaat
     1501 tgctgctccc aagttctgaa acattgcaat gcctcccgac cccgccgccc gcgtgccgtg
     1561 atcacagtag cactgtttcc gtctttctcc aatgtgcgat gatagagttc ggcctacgcg
     1621 ggccccgtga ccggctcctg ttgggtgatg cagtggatgt tgccgccggc gtagagaagg
     1681 gcgcgggagg ggacggggac gatctccaga tctttgaaca gctcgcggaa a
//
LOCUS       DAORVN010000533         1726 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_79633_length_1726_cov_2.865949, whole genome shotgun sequence.
ACCESSION   DAORVN010000533 DAORVN010000000
VERSION     DAORVN010000533.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1726
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79633_length_1726_cov_2.865949"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..277)
                     /locus_tag="PKV23_09930"
     CDS             complement(<1..277)
                     /locus_tag="PKV23_09930"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009210170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HQX85795.1"
                     /translation="MSRSLLASVFLPSPNIEARRDGIRPDMLVLHYTGMPDARKACRW
                     LCDPVSKVSCHYLVDENGAVTQMVDEGMRAWHAGVSSWKGREDLNSCS"
     gene            complement(274..963)
                     /locus_tag="PKV23_09935"
     CDS             complement(274..963)
                     /locus_tag="PKV23_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013419346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DjiA"
                     /protein_id="HQX85796.1"
                     /translation="MSIWTRISEALALVGDSIGGFLAKLAKGRARAPEKSLGFTIGMI
                     ALGAKMAKADGVVTVDEVAAFKQVFHVPQAELAAVARVFNLAKQEVTGYDTYARQIAR
                     LFHARAATLESILDGLFHIAKADGTIHPAELNYLENVARLFGFGPHDFARIKARHVSA
                     GADDPYIILDVDRKMDLATIKLRYRKLVRDNHPDKQIAAGVPAEMVVLATSRLARINA
                     AYEQILKEHQL"
     gene            complement(1008..1340)
                     /locus_tag="PKV23_09940"
     CDS             complement(1008..1340)
                     /locus_tag="PKV23_09940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85797.1"
                     /translation="MEQELIKPDLPPTGSSQAFSPIGCFIAAFGITLLTFCLLGTAAM
                     TTIWAFAKLFGLPEVVSEALMVLGLIPAVLASIWTAGRAWHVERRLAQNLDIDVPNFK
                     LLHYFKKN"
     gene            complement(1403..>1726)
                     /locus_tag="PKV23_09945"
     CDS             complement(1403..>1726)
                     /locus_tag="PKV23_09945"
                     /inference="COORDINATES: protein motif:HMM:NF017319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HQX85798.1"
                     /translation="SRYYQGVVTFMSRYFAEQSREYRVAKYEIGEATVDNNKDVIVAS
                     KIYLLSGQTYNVRWKLTWRNGTYKVADVKVMGFSLTYLQRGLFTSYISKRKGDVSQLV
                     AALTR"
BASE COUNT          388 a          501 c          480 g          357 t
ORIGIN      
        1 tggaacagga attgagatcc tcccggccct tccatgatga aacaccagca tgccaggccc
       61 gcatgccttc atccaccatc tgggtgacgg cgccgttttc atcaaccaga tagtgacagg
      121 aaactttcga aacgggatcg cacagccagc ggcaggcttt ccgcgcgtca ggcatgcccg
      181 tgtaatgcag gacaagcatg tcaggccgga tgccatcccg ccgcgcttct atgttgggcg
      241 acggcagaaa cactgaagca agcagcgagc ggctcacaac tgatgctcct tcaggatctg
      301 ctcataagcc gcattgatgc gggccaggcg gcttgttgcc agaaccacca tttccgccgg
      361 aacacccgcg gcgatttgct tgtccggatg attgtcgcgc accagcttgc gatagcgcag
      421 cttgatcgtg gccagatcca ttttcctgtc cacatccaga atgatgtatg gatcatcagc
      481 gccggcactc acatggcgcg cctttatgcg ggcaaaatca tgggggccga atccgaaaag
      541 ccgcgcgacg ttttcaagat agttcaattc tgccggatgg atggtgccgt ccgccttcgc
      601 aatatggaag agtccgtcga gaatactttc aagcgtggca gcccttgcat ggaacagccg
      661 ggcaatctgg cgcgcatagg tatcataccc cgtgacctcc tgctttgcca gattgaacac
      721 cctggccacg gccgcaagct ccgcctgcgg aacatgaaac acctgtttga aggccgccac
      781 ttcatcaacc gtcaccaccc cgtcggcttt tgccatcttg gcgccgaggg ctatcatccc
      841 gatggtgaac cccagggatt tctcaggcgc gcgagcccgc cccttggcga gctttgccag
      901 aaagccgcca atcgaatctc caacgagggc aagcgcctca ctgatgcggg tccatataga
      961 catagggtgg tgagtttacc gaatgcgggg ccgggcttaa ggggcaatca attctttttg
     1021 aaatagtgca agagcttgaa gttggggaca tcgatatcca ggttctgggc gagcctccgt
     1081 tccacgtgcc aggcccgtcc ggcggtccag atggatgcaa gtaccgcggg aattaggccg
     1141 agcaccatga gtgcttctga cacgacctca ggcaatccaa acagcttggc gaatgcccag
     1201 atcgtggtca ttgctgccgt gcccaaaagg cagaaggtga gcagcgttat tccgaaagct
     1261 gcaataaagc agccaatcgg gctgaaagcc tgtgaagaac ctgttggcgg cagatccggc
     1321 ttaatcaatt cctgttccat ggggctctca tatgcccttt cgggccggaa attgcaacga
     1381 acccggaatt gatggccagc gctcagcgtg tgagggcggc caccagctgg ctgacatcac
     1441 ccttgcgctt ggaaatatag gaggtgaaca gtccgcgctg caagtaagtg agagagaagc
     1501 ccataacctt gacgtccgcg accttgtaag ttccattgcg ccaggtgagc ttccagcgca
     1561 cgttgtaagt ctgccccgac agcaggtaaa ttttgctggc gacgatgaca tccttgttgt
     1621 tgtcgacggt ggcctcgcca atctcatatt ttgccacccg gtattcgcgc gactgctcgg
     1681 caaaataccg cgacatgaag gtaacgacgc cctgataata ccggct
//
LOCUS       DAORVN010000534         1725 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_79709_length_1725_cov_2.792814, whole genome shotgun sequence.
ACCESSION   DAORVN010000534 DAORVN010000000
VERSION     DAORVN010000534.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1725)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1725)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1725
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79709_length_1725_cov_2.792814"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            91..915
                     /locus_tag="PKV23_09950"
     CDS             91..915
                     /locus_tag="PKV23_09950"
                     /inference="COORDINATES: protein motif:HMM:TIGR03709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2 family protein"
                     /protein_id="HQX85799.1"
                     /translation="MAEKKSFASKFIAVPGKKFSLGALDPRDDSAFDDKDKAKEQLLR
                     DAVAIDGLQDRLFAEGKRALLVVLQGIDCSGKDGTVRAVFNPCGPLGVTVKPFGVPSE
                     VERAHDYLWRVHKACPAKGYIGIFNRSHYEDVLVVKVEKLAAADAIERRYDEINAFEK
                     LVANSGTRILKFMLHVSKEEQAVRLQERVDDPAKQWKFKVSDLATRAKWADYMAAYET
                     AISRCSTEHAPWHIIPADRNWARNAAIARIVRETLEEMNPQYPLPKDWDPKTIKVV"
     gene            complement(902..1531)
                     /locus_tag="PKV23_09955"
     CDS             complement(902..1531)
                     /locus_tag="PKV23_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010658172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HQX85800.1"
                     /translation="MPHTKAKPVVNTRIQARNKELILEAALDVFSASGFRGATVDQIA
                     AKAEMSKPNLLYYFRRKEDIYVALLEHTLSDWLEPLRELDPAGDPLTELARYISAKMQ
                     MSEANPKASRLFANEILHGAPMIEKFLKGPLRQLVAEKSQVIQSWIAAGKLAPVDPYH
                     LIFAIWAVTQHYADFSVQVDAVLGRREDPKAAQKAVLEILLRGLQVKQP"
BASE COUNT          374 a          436 c          549 g          366 t
ORIGIN      
        1 tgacgggcgt tctttcaagg caaatccgga agttgtttga ttgtggggaa gggtagctgc
       61 tattgaaggg gaatagaaga ggacaggatt atggccgaga aaaaatcatt tgcatcaaag
      121 tttatcgctg tgccgggcaa gaagttttcg ctcggcgcgc ttgatccgcg cgatgacagc
      181 gcctttgatg acaaggacaa ggccaaggag cagctgctta gggatgcggt ggccattgac
      241 gggctgcagg accggctttt cgccgagggc aagcgggcgc ttctggttgt cctgcagggg
      301 attgactgtt cgggcaagga cggcacggtg cgggccgtgt tcaatccctg cgggccgctg
      361 ggagtgacgg tgaagccgtt cggcgttccg tccgaggtgg agcgggccca tgattatctg
      421 tggcgggttc ataaggcctg cccggccaag ggctatatcg gcatcttcaa ccgttcccac
      481 tatgaggacg tgctggtggt gaaggtcgaa aaactggcag ccgccgatgc gatcgagcgc
      541 cgctatgacg aaatcaacgc ctttgaaaag ttggttgcca attccggcac gcggatcctg
      601 aaattcatgc tgcatgtttc aaaggaggaa caggcggtgc gcctgcagga gcgggttgat
      661 gatcctgcca agcagtggaa attcaaggtg agtgatcttg ccacgcgggc caagtgggcg
      721 gactacatgg cggcctatga gacagcgatc tcgcgctgct cgaccgaaca tgcgccgtgg
      781 cacatcattc cggcggaccg caactgggcg cgcaatgcgg ccatcgcgcg gattgtgcgc
      841 gagacgctgg aggagatgaa cccgcaatat ccgctgccga aggactggga tccgaaaacc
      901 attaaggttg tttgacctga aggccgcgca ggagaatttc cagcacggct ttctgcgcgg
      961 cctttggatc ctcgcggcgg cccagaaccg catcgacctg cacggaaaag tcggcgtagt
     1021 gctgggtcac ggcccagatg gcgaagatca gatggtaggg atcgacggga gcaagctttc
     1081 ccgccgcgat ccaggactgg atgacctggg atttttcggc caccaattgc cgcagcgggc
     1141 ccttgagaaa tttctcgatc atcggcgcgc cgtggaggat ttcgttggcg aagagccttg
     1201 aggcctttgg atttgcttcc gacatctgca tcttggcgga gatgtagcgg gcgagttcgg
     1261 tgagcggatc gcccgccgga tcaagctcgc gcaggggctc cagccagtcg ctcagcgtat
     1321 gctcgaggag cgccacatag atgtcttcct tgcggcggaa gtaatagagc aggttgggct
     1381 tggacatttc ggccttcgcg gcgatctggt ccaccgtggc gccgcgaaag ccggaagccg
     1441 agaaaacatc aagtgccgcc tcgaggatca gctccttgtt gcgggcctga atgcgggtat
     1501 tgaccaccgg ttttgctttg gtatgtggca ttgcggatac cttgataatt cttgattaaa
     1561 aggtcaagga taatcagcaa caagcgctga caagaaagtt gcggttttat tggcttttcc
     1621 gtattgtcac gaccattgca cacaattgct gacaatcatc aaacgtacaa agcccttaca
     1681 aatgacaaaa ccattcgctt tcaccaagac gttcattgaa cgcgc
//
LOCUS       DAORVN010000535         1721 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_80034_length_1721_cov_3.151261, whole genome shotgun sequence.
ACCESSION   DAORVN010000535 DAORVN010000000
VERSION     DAORVN010000535.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1721
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80034_length_1721_cov_3.151261"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1721)
                     /locus_tag="PKV23_09960"
     CDS             complement(<1..>1721)
                     /locus_tag="PKV23_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014527141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033225 - ATPase-coupled
                     2-aminoethylphosphonate transporter activity [Evidence
                     IEA];
                     GO_process: GO:0033223 - 2-aminoethylphosphonate transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     permease subunit"
                     /protein_id="HQX85801.1"
                     /translation="KSMTTYSFNLASFEMQVDAGQGWQPPQKISELAAQMDPARLPKL
                     SSSTDGRLAVVDFVQGYSFRGKEVYRFRNAEEGAFHLAGSLRIADMDWHEYPANDFRK
                     VMMRPAPSRGLDNYVTYFSTPSLVNSINNSLFIAAISTAFTVVLAFGFAYALAMTVMP
                     FKGLFRLIVMIPILVPSLLPGIALVYMFGKQGFLTPLLMGHSIYGPIGIVIGSVFFSF
                     PHAFIIIATALSLSDARLHEASIALKASPLKRFLSVTLPGARYGLISATFVTFTLVIT
                     DFGLPKVIGGQFNVLAVDIYKQVIGQQNFEMGAVVSVVLLIPAVIAFAVDRIVQRRQQ
                     STLSARSVPFAPTPNPLADRLALLFCILVAVFIAGILLVCQFAALVKLWPYDLSFGLR
                     NYDFSRTDGGGWEAYFNSMRMALYVALAGTGIVFIGAYIVEKLQGMKAGRSLLHMLAI
                     MPLAVPGMVLGLSYIFFFNDPANPLNFLYGTMAILVISSITHFYTVSHLTAVTALKQL
                     DPEFESVSASLKQPAWRLFSRVTVPVCMPPLLDISIYLFVNAMTTVSAVVFLYSPATK
                     LASVAVL"
BASE COUNT          392 a          446 c          614 g          269 t
ORIGIN      
        1 tgagcaccgc cacggaggcg agcttcgtag ctggcgaata gaggaacacc acggcggaca
       61 cggtggtcat ggcattgacg aagagataga tcgagatgtc gagcagcggc ggcatgcaga
      121 cgggcacggt gacccggctg aacaggcgcc aggcgggctg tttcagcgag gccgagacac
      181 tttcgaattc cggatcgagc tgcttcagcg cggtgaccgc ggtgaggtgc gagaccgtat
      241 agaagtgggt gatcgagctg atgacgagga tggccatggt gccatagagg aagttcagcg
      301 ggttggccgg gtcgttgaag aagaagatgt aggagaggcc aaggaccatg ccgggaaccg
      361 ccagcggcat gatggccagc atgtggagca ggctgcggcc ggctttcatg ccctggagct
      421 tttcaacgat gtaggcgccg atgaagacaa tgccggtgcc ggccagcgcc acataaagcg
      481 ccatgcgcat ggaattgaaa taggcttccc agccgccgcc gtcggtgcgg ctgaaatcat
      541 agttgcggag gccgaagctc aggtcatagg gccagagctt caccagggcg gcgaactggc
      601 agacgagcag gatgccggcg atgaacaccg ccaccaggat gcagaacagc agcgccagcc
      661 ggtcagccag gggatttgga gtcggcgcga agggcactga ccttgccgaa agcgtcgact
      721 gctggcggcg ctggacaatg cggtccacgg cgaaggcgat gaccgccggg atcagcagca
      781 cgacggagac caccgcgccc atttcgaaat tctgctggcc gatgacctgc ttgtagatat
      841 ccaccgccag cacgttgaac tggccgccga tcaccttggg caggccgaag tcggtgatga
      901 ccagggtgaa ggtgacgaaa gtggctgaga taaggccgta gcgggcgccg ggcagcgtga
      961 cgctgagaaa gcgttttaac gggctggcct tgagcgcgat cgaggcttcg tgcaggcggg
     1021 cgtcagacaa ggacagggcg gtggcgatga tgatgaaggc gtgcggaaag gaaaagaaca
     1081 ccgagccgat gacgatgccg atggggccat agatcgaatg gcccatgagc agcggggtga
     1141 gaaagccctg cttgccgaac atgtagacga gcgcgatgcc gggaagcagc gagggcacca
     1201 gaatggggat catcacgatg agccggaaca ggcccttgaa gggcatcacg gtcatggcca
     1261 gcgcataggc aaagccgaag gccaggacca cggtgaaggc agtgctgatg gcagcgatga
     1321 acagcgagtt gttgatcgag ttgaccagcg agggggtgga gaaataggtg acgtaattgt
     1381 cgaggccgcg gctgggggcg ggccgcatca tgaccttgcg gaaatcattg gccggatatt
     1441 cgtgccaatc catgtctgca atgcggagcg agcccgcgag atggaaggcg ccctcctccg
     1501 cattgcggaa gcggtagacc tctttgccgc ggaaactgta gccctgaaca aaatcgacga
     1561 ccgcgaggcg gccatcggtg ctggaactga gtttcggcag cctggcggga tccatttgcg
     1621 ccgccagctc cgaaatcttc tgcggcggct gccagccctg cccggcatcc acctgcattt
     1681 caaaggaggc aaggttgaag ctgtaggtgg tcatggactt g
//
LOCUS       DAORVN010000536         1720 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_80098_length_1720_cov_4.255255, whole genome shotgun sequence.
ACCESSION   DAORVN010000536 DAORVN010000000
VERSION     DAORVN010000536.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1720)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1720)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1720
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80098_length_1720_cov_4.255255"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="PKV23_09965"
     CDS             complement(<1..300)
                     /locus_tag="PKV23_09965"
                     /inference="COORDINATES: protein motif:HMM:NF018875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis HemY N-terminal
                     domain-containing protein"
                     /protein_id="HQX85802.1"
                     /translation="MIKLLWRFGLLIVAALFFTWLADRPGTVTIQWMGREIQMAFVIA
                     VALAILTLALLWFVWGFFRRIWRSPTTARDYWRFRKNRKAYESLSRGIIAAGAGDS"
     gene            complement(297..1406)
                     /locus_tag="PKV23_09970"
     CDS             complement(297..1406)
                     /locus_tag="PKV23_09970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85803.1"
                     /translation="MTHDDPESPLKPQVIDLEAEDVVMEETRASETPPPPSPPKKSGF
                     PAAWLAIAVVGGAIAGGWLYRDVLSSYLPSNELVTAQTRIDVLEAQSKTLTEQLASIA
                     ATSGQLSAEIQGATEKSSAALSASAAVEKRLAAAESATQAARAELEKLKSAVASGGAS
                     GATVDSSALAVLAQRLDVLEKDVASLKTVSAPTDQSTVTATLSQSLSDLKAKIAAGAP
                     YRAELERISRMVPAAAGLDTLSANANEGLPTAAGLAAELTGLIPLLPKPEITLPSSEN
                     SYLDSFWDAMKNVITIRRIGDTDWPDLAAKCAALAESGDLAQAIERIDAAEGAKPAAL
                     SGWRDRAAARIAIEAALEQTSQAVLRQITSMGATP"
     gene            complement(1434..>1720)
                     /locus_tag="PKV23_09975"
     CDS             complement(1434..>1720)
                     /locus_tag="PKV23_09975"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HQX85804.1"
                     /translation="ASGFAVEKVVTYDALVQTPDDIEGALKRADGVLLYSPRTAHLWC
                     KLMVSSGLEREAAAPIYYCLSENVAKALPGDWKRRVAEMPDEAAMLALLD"
BASE COUNT          329 a          503 c          540 g          348 t
ORIGIN      
        1 gctgtcgccg gcgccggccg cgatgatgcc gcgcgacagc gattcatagg ccttgcggtt
       61 tttgcgaaaa cgccaatagt cgcgcgccgt ggtggggctg cgccagatcc tgcggaaaaa
      121 gccccagaca aaccagagca gcgcaagggt aagaatggca agagcgactg caatcacaaa
      181 cgccatctgg atttcgcggc ccatccattg aatggtgacg gtgccgggcc ggtcagccag
      241 ccaggtgaag aagagggcgg cgacaatcag gaggccgaag cgccagagca gcttgatcat
      301 ggcgtggcgc ccatgctggt gatctggcgc aggactgctt gcgaagtctg ttcgagggcc
      361 gcttcaattg caatgcgcgc cgcggcgcgg tcgcgccagc ctgacagggc agcgggcttt
      421 gcgccttccg ccgcatcaat tctttcaatg gcctgggcta aatcgccgct ttctgcaagg
      481 gcggcacatt tcgcggcaag gtccggccag tcggtatcac cgatgcggcg gatggtgatc
      541 acattcttca tggcatccca gaaactgtcg aggtaggaat tttccgatga cggcaaggta
      601 atttccggtt tgggcagtaa tggaatcagt cctgtcagct cggccgccag gcccgcagca
      661 gttggcaggc cttcgttggc gttggcggac aatgtgtcga gcccagcggc agcgggcacc
      721 atgcgcgaaa tacgctcaag ctctgcacga tatggagcgc cggcggcgat cttggctttg
      781 agatcggaca gggactggga gagggttgcg gtgactgtgg attgatcggt tggcgcgctg
      841 accgttttca ggctggcaac atctttttcc agcacgtcga ggcgctgggc caggaccgcc
      901 aaggcgctgg agtccactgt tgcaccagat gctccgccac tggcaactgc agacttcagt
      961 ttttccagct ccgcccttgc cgcctgcgtt gccgattcgg cggcagcaag acgtttctcc
     1021 accgctgcag atgcgctcag cgcggcggac gacttttccg ttgcgccctg gatttcggca
     1081 ctcagctgac cggacgtggc ggcaatggac gcaagctgtt cagtcagcgt cttgctctgg
     1141 gcctccagga catcgatgcg agtttgggct gtcaccagtt cattgcttgg caagtaggat
     1201 gacaggacat ccctgtagag ccagcccccg gcaatcgccc cgccaactac agcaatggca
     1261 agccaggctg ccggaaaccc cgatttcttc ggtggcgatg gcgggggcgg ggtttcggaa
     1321 gccctcgtct cttccataac cacgtcctcg gcctccaggt cgataacctg cggtttcagg
     1381 ggcgattcag gatcgtcatg ggtcatgtca caggcctaac gggttcgcgg gcgtcaatca
     1441 agaagggcca gcatcgcagc ttcatccggc atttctgcga cacgtctttt ccagtctccg
     1501 ggcagggctt ttgccacatt ttccgagagg caatagtata tgggggcggc ggcttctctt
     1561 tccaatcctg aacttaccat cagcttgcac cagagatgcg ccgtgcgggg cgagtagagc
     1621 agaacgccat cggcacgctt cagcgcccct tcgatatcat caggggtttg aacgagggca
     1681 tcataggtga cgactttttc caccgcgaaa ccggacgcca
//
LOCUS       DAORVN010000537         1719 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_80223_length_1719_cov_2.594952, whole genome shotgun sequence.
ACCESSION   DAORVN010000537 DAORVN010000000
VERSION     DAORVN010000537.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1719
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80223_length_1719_cov_2.594952"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /locus_tag="PKV23_09980"
     CDS             <1..333
                     /locus_tag="PKV23_09980"
                     /inference="COORDINATES: protein motif:HMM:NF018070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcB family DNA repair protein"
                     /protein_id="HQX85805.1"
                     /translation="LATGRRADVIALGDSGAIWIIEIKSSLLDFKVDQKWPEYRDFCD
                     RFFFAIPQTLDPEIIPYEAGLIVADNWGAEILRQSGETTLHASRRKAVTLAFARVSSQ
                     RLHSLYDP"
     gene            complement(334..879)
                     /locus_tag="PKV23_09985"
     CDS             complement(334..879)
                     /locus_tag="PKV23_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ActR/PrrA/RegA family redox response regulator
                     transcription factor"
                     /protein_id="HQX85806.1"
                     /translation="MTEIDLPADKTLLLVDDDKPFLTRLARAMETRGFEVRTAESVAE
                     GIQQVRKAAPGYAVVDLRLSDGNGLDVIEALHASRPEARVVVLTGYGNIATAVTAVKL
                     GAIDYLAKPADADAVYGALISDKDSRSALPENPMSADRVRWEHIQRVYELCNRNVSET
                     ARRLNMHRRTLQRILAKRAPK"
     gene            complement(935..>1719)
                     /locus_tag="PKV23_09990"
     CDS             complement(935..>1719)
                     /locus_tag="PKV23_09990"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQX85807.1"
                     /translation="EEARQMSSALTAAELVLAREQRLSALDGLAAAAAHELGTPLATI
                     ALVAKELKRELPAKGPHTEDIDLLISQTGRCREILSRLANRDTQADDMYARIKLSVMI
                     EDLVAPIRGPDVTIDITVAGDGTAGQSPEPVFRRNPAIAYGLANLLENAVDFAQTKVS
                     IVAQWSKLKVSLLISDDGPGFEQGIFDRLGDPFVTTRPGYGVDDEGEKGRHEGMGLGF
                     FIAKTLLERSGATVSLANRPLPEHGAVIQLSWPRSTVDLGSP"
BASE COUNT          295 a          567 c          505 g          352 t
ORIGIN      
        1 ctcgccaccg gccgccgcgc cgatgtgata gcgcttggcg acagcggcgc catctggatc
       61 atcgaaatca aatcctcgct cctggatttc aaggttgacc agaaatggcc ggagtaccgc
      121 gacttctgtg accggttttt cttcgccatt ccgcaaaccc ttgatcccga gatcattccc
      181 tatgaggcgg gcctcatcgt ggcagacaat tggggagcgg agattcttcg acagtccggc
      241 gagaccacgc ttcacgcctc aaggcgcaag gccgtgaccc tggccttcgc ccgcgtctcc
      301 agccagcgcc tgcacagtct ttatgatcct tgattactta ggtgcccgtt tggccaggat
      361 gcgctgcagc gtgcgccggt gcatgttgag ccggcgtgcc gtttccgaga cattgcggtt
      421 gcacaactca tagacacgct gaatgtgctc ccagcgcacc cggtccgccg acatggggtt
      481 ctccggcagc gctgagcggc tgtccttgtc ggagatgagc gcgccgtaaa ccgcatcggc
      541 atcggcgggc ttggccagat agtcgatcgc ccccagcttc accgccgtca ccgccgtggc
      601 gatatttccg tagccggtaa gcactacaac ccgcgcctcc ggcctgctgg catgcagggc
      661 ctcgatcacg tccagcccat tgccgtcgct gagtcgcagg tcaacgaccg cataacccgg
      721 cgccgctttc ctcacctgct ggatgccttc ggccacggac tcggccgtcc gcacctcaaa
      781 accgcgggtt tccatggctc gcgccagccg ggtcagaaac ggcttgtcgt catcgacaag
      841 cagaagtgtc ttgtcggctg ggagatcgat ttcagtcatg gtggttctct tattgacacc
      901 cttatacgag tcaagggctc ccttggacat ctccctatgg gctgcccagg tccaccgtgc
      961 tccgcggcca gcttagctgg atgacggcgc catgctcggg gaggggccgg ttggccaggg
     1021 aaactgtggc gcctgagcgc tccagcaggg tcttggcaat gaaaaagccc agtcccatgc
     1081 cctcgtggcg tcccttttcg ccctcatcgt ccaccccata gcctggccgc gttgtcacga
     1141 agggatcgcc gaggcggtcg aaaatgccct gctcaaagcc cggcccgtca tcggaaatga
     1201 gcagcgacac cttcagcttg gaccattgcg caacaatgct caccttcgtt tgggcaaaat
     1261 ccacggcgtt ttccagcagg ttcgccaggc cataggcaat cgccggattc cgccggaaca
     1321 cgggctcggg cgattgcccg gccgttccgt cgccggcaac cgtgatatcg atggtgacat
     1381 ctggtccgcg gatcggcgcc accagatcct cgatcatcac cgaaagcttg atccgcgcat
     1441 acatgtcgtc ggcctgcgtg tcccggttgg caagccgtga caggatttcc cggcacctcc
     1501 ctgtctggct gatgaggaga tcgatatcct ccgtatgtgg gcctttcgcc ggcagttccc
     1561 gcttcagttc cttggcgaca agcgcgatgg tggccagcgg cgttcccagt tcgtgggctg
     1621 ccgcggcagc caaaccgtca agcgccgaaa ggcgctgttc gcgcgccagc accagttcgg
     1681 cggcggtgag tgctgatgac atttgccggg cttcttccg
//
LOCUS       DAORVN010000538         1715 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_80590_length_1715_cov_2.322892, whole genome shotgun sequence.
ACCESSION   DAORVN010000538 DAORVN010000000
VERSION     DAORVN010000538.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1715
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80590_length_1715_cov_2.322892"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..711
                     /locus_tag="PKV23_09995"
     CDS             <1..711
                     /locus_tag="PKV23_09995"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HQX85808.1"
                     /translation="CPRTEATGYSKPGGYAEYMVAPAAFCGHLPEHVDMHAMAPILCA
                     GVTTYRGIKRTGARPGQWLAVIGIGGLGHIAVQYAKAMGLRVAAVDVLTEKLNHAKRL
                     GAEVLIDAREGDPGAALREKSGGGAHGAIVTAVSPRAFEQSVTMLRPAGTVSYIGLPG
                     RDADQIRLSISSIVNSEITVRGSNVGTRQDLNEAIAFAANGLVKADIETQPLENINAI
                     FARMKKGEITGRVVLDIS"
     gene            901..>1715
                     /locus_tag="PKV23_10000"
     CDS             901..>1715
                     /locus_tag="PKV23_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HQX85809.1"
                     /translation="MATPKQVSEAPDDPAIQAGGHVPPSFWILALGSIGVVYGDIGTS
                     PLYAIRESVLAATGKSGPITEPVVLGVLSLIIWALILVVTVKYVFVLLNADNKGEGGT
                     LSLMALAQRVMGSNMVLIPILAMIGAALFYGDAMITPAISVLSAIEGLKVITPALEPY
                     VLPITLAIIVALFSVQARGTASVASLFGPITLVWFIALALSGLAHIVDYPAILLSFNP
                     IYAVTFLGSHGIIGLVTLGAVFLAVTGAEALYADLGHFGKRPIQLAWIIVVFPA"
BASE COUNT          348 a          550 c          446 g          371 t
ORIGIN      
        1 tgcccccgaa ccgaagccac tggatatagc aaacccggtg gttatgccga atacatggtg
       61 gcgcctgccg ccttttgcgg ccatttgcct gaacatgtcg acatgcatgc catggccccc
      121 atcctctgtg ccggtgtcac cacctatcgc ggcatcaagc gcacgggcgc aaggcccggc
      181 cagtggctgg cggtgatcgg cattggcggc ctcggccaca tcgccgtgca atatgcaaag
      241 gccatgggcc tgcgcgtcgc cgccgtggat gtcttgaccg agaaattgaa tcacgccaaa
      301 cggctgggcg ccgaagtcct gatcgatgcc cgcgaaggcg atcccggcgc ggccctcagg
      361 gaaaaatccg gcggcggcgc ccatggggcc atcgtcaccg ctgtgtcgcc ccgcgccttt
      421 gagcaatccg tcaccatgct caggcccgcc ggcaccgtgt cctacatcgg ccttcccggc
      481 cgcgacgccg accagatccg cctgtcgatt tccagcatcg tcaacagcga aataactgta
      541 cgcggatcaa atgtcggaac gaggcaggat ctgaacgaag ccatcgcctt tgccgccaac
      601 ggtctggtga aggccgacat cgaaacccag cccctggaaa acatcaacgc catcttcgcc
      661 cgcatgaaaa agggtgaaat taccggaagg gttgtgctgg acataagtta accgcatgaa
      721 tgctaaactt cataaaactc ggattaacgt ttaatatcaa tagaatatgc gtgtatgtca
      781 gtattcgtaa cctttccagc attacacccc agccatgccc actttgcatg aacggcgcac
      841 gccaaaaggc ctaaaccacc tcccctttgc tgcaccgcag caaatcaatg gagaaatgaa
      901 atggcaacac ccaaacaagt gagcgaggca ccagacgatc ccgcaattca ggccggcggc
      961 catgtgccgc caagtttctg gattctcgcg ctgggatcca tcggcgttgt ttacggcgat
     1021 atcggaacca gtcctcttta cgcaatacgc gagtcggtgc tcgccgccac cggcaagtcc
     1081 ggacccatta ctgagcccgt cgttttagga gtcctctctc tcatcatctg ggccctcatc
     1141 ctcgtcgtca ccgtgaagta tgtttttgtt ctgctgaatg ctgacaacaa gggcgaaggc
     1201 gggaccctgt cgctcatggc gctggcccag cgcgttatgg gcagcaacat ggtgcttatt
     1261 cccattctcg ccatgatcgg tgcggcgctg ttttatggcg acgcaatgat cactccggca
     1321 atttccgtac tctccgccat cgagggcctc aaggtcataa cgccggcact tgaaccctat
     1381 gtgctgccga taaccctggc gatcatcgtc gccctgttct cggtccaggc gcgcggcacc
     1441 gcaagtgtgg cctcactgtt cggtcccatc acactcgtct ggttcattgc attggctttg
     1501 tctggccttg cacacatagt cgactatccc gccatcctgc tttcattcaa cccgatttat
     1561 gcagtgacgt tccttggctc gcacgggatc attggccttg tcacgctcgg tgccgtcttc
     1621 ctcgccgtca ccggcgcgga agccctctac gccgatctcg gccattttgg caaacgtccc
     1681 attcaactgg cgtggatcat tgtcgttttc cctgc
//
LOCUS       DAORVN010000539         1713 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_80738_length_1713_cov_2.773221, whole genome shotgun sequence.
ACCESSION   DAORVN010000539 DAORVN010000000
VERSION     DAORVN010000539.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1713
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80738_length_1713_cov_2.773221"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1222)
                     /locus_tag="PKV23_10005"
     CDS             complement(<1..1222)
                     /locus_tag="PKV23_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HQX85810.1"
                     /translation="MRLRLLGIALCACAAQFLASYSQATPSHGISVFGELKYPASFSH
                     FDYVNPDAPKGGRMALIGIVANDTFDSFNGYILKGDAAQGLAMLFDTLMARAMDEPDA
                     VYGLVAKTADLAADRKSLVFELRPEAKFADGAALTSEDVCDSFRLLSTLGHENIRLTI
                     KDVEACDEVAPHTVRYRFKSGGIRDLPLSIAALPIFSKAYYAQHDFATTSLEAPLGSG
                     PYKIKSYRTGEYVAYDRRADYWAKDLPVNRGRHNLDEVRFDYFRERTAGFEALKSGVI
                     DLREEYTSRDWATAYDFPAIKDGRVKQTILPDETPSGAQGYFFNLRREKFQDIRVRRA
                     FNLAFDFEWKNKNLFYGLYDRTASFFESSPLKAVGAPPAEELAILEPMRATLRPEVFL
                     TPELPPVSDGSGRDR"
     gene            complement(1219..>1713)
                     /locus_tag="PKV23_10010"
     CDS             complement(1219..>1713)
                     /locus_tag="PKV23_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019199856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HQX85811.1"
                     /translation="DSPTCERITLPYKQQLELLGIGVSIKTVDGAQYERQTQTFDYDI
                     IVGNWGQSLSPGNEQRDFWGSAAADTNGSRNYIGIKNPVIDKLIEAIIFAKDREGLTT
                     ACKALDRALIWNHYLVPHWYVAYERTARWDRFGKPETLPDYSIGFPTVWWWDKDKAAK
                     VAKG"
BASE COUNT          338 a          515 c          506 g          354 t
ORIGIN      
        1 tgcggtcacg gccggaacca tcgctgaccg gcggcagttc gggcgtcaaa aaaacttccg
       61 gccgcaacgt ggcgcgcatc ggttcgagaa tggcaagctc ttcggcaggc ggcgcgccga
      121 ccgctttcag tggtgacgat tcaaagaagc tcgccgtgcg gtcatagaga ccatagaaaa
      181 ggttcttgtt tttccactca aaatcaaagg ccaggttgaa ggcccggcgc acccggatgt
      241 cctggaattt ctcccgccgc aggttgaaga aatatccttg tgcccctgaa ggcgtctcat
      301 ctggcaagat ggtctgcttg acccggccat ccttgatcgc cgggaaatca taggccgtgg
      361 cccagtcgcg cgaggtgtat tcctcgcgaa ggtcgatcac gccggatttg agcgcttcga
      421 agcccgccgt gcgctcgcgg aaatagtcaa agcgcacctc gtccaggtta tgccgcccgc
      481 ggttgaccgg aaggtccttg gcccaatagt cggcacgccg gtcgtaggcc acatattcac
      541 cggtgcggta gcttttgatt ttgtagggcc cggagcccag aggcgcttcg aggctggtcg
      601 tggcgaaatc atgctgggca tagtacgcct tggagaaaat cggcagggcc gcaatgctca
      661 gcggcaggtc gcggatgccg ccgctcttga agcggtagcg caccgtatgt ggcgcgacct
      721 catcgcacgc ctccacatcc tttatcgtga gcctgatgtt ttcgtggcca agagttgata
      781 gcaggcggaa actgtcgcag acatcttcgg aggtaagcgc cgcgccatcg gcaaatttcg
      841 cttccggacg cagctcgaaa accaggctct tgcggtccgc cgccagatcg gcggtttttg
      901 ccaccagccc gtaaaccgca tccggctcgt ccatggcccg cgccatgagc gtgtcgaaca
      961 gcatggccag cccttgggcc gcatcaccct tgaggatgta cccgttgaag ctgtcgaagg
     1021 tgtcattggc cacgatgccg atcagcgcca tgcgcccgcc cttgggcgca tccgggttta
     1081 cgtaatcaaa atggctgaaa ctggccgggt acttgagttc gccgaatacc gaaattccgt
     1141 ggctcggcgt ggcctggctg taagatgcaa gaaactgtgc cgcgcatgcg cagagagcga
     1201 tgccgagaag gcgcagcctc atcccttcgc caccttcgct gccttatcct tgtcccacca
     1261 ccagaccgtc gggaagccaa tggaataatc cggcaatgtc tccggctttc caaagcggtc
     1321 ccagcgcgcc gtgcgttcgt aagcaacata ccagtgggga accagatagt gattccagat
     1381 cagggcccgg tcgagcgcct tgcaggccgt ggtcaatccc tcccggtcct tcgcgaagat
     1441 gattgcttca atgagcttgt cgatgaccgg attcttgatg ccaatataat tgcggctgcc
     1501 gttggtatcg gccgccgccg aaccccagaa atcacgctgc tcattgcccg gcgacaggga
     1561 ctgcccccag ttgcccacga tgatgtcata gtcaaatgtc tgcgtctggc gttcatactg
     1621 ggcaccgtcg accgtcttga tgctgacgcc gatgcccagc aattccagct gctgcttgta
     1681 gggcagtgta atgcgttcac aagttgggga gtc
//
LOCUS       DAORVN010000540         1706 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_81299_length_1706_cov_3.737129, whole genome shotgun sequence.
ACCESSION   DAORVN010000540 DAORVN010000000
VERSION     DAORVN010000540.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1706
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81299_length_1706_cov_3.737129"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..451)
                     /gene="nth"
                     /locus_tag="PKV23_10015"
     CDS             complement(<1..451)
                     /gene="nth"
                     /locus_tag="PKV23_10015"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012112426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HQX85812.1"
                     /translation="MAKARRLKPPEIEEIFRRFHDIEPEPKGELNSVNEFTFLVAVVL
                     SAQATDMGVNRATSALFKAVDTPEKMLALGEEGLRDYIKSIGLYRAKARNVIALSKIL
                     VEKHGGKVPGEREALEALPGVGRKTANVVLNIVFGEPTIAVDTHLFRL"
     gene            492..968
                     /locus_tag="PKV23_10020"
     CDS             492..968
                     /locus_tag="PKV23_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002717760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2244 domain-containing protein"
                     /protein_id="HQX85813.1"
                     /translation="MDDTKPKTWRAILTPHRSLSRKGFIILMLVIAGFNFTLGMVFYL
                     IGAWPVLGFMGLDVALILWAFRANFADARRAEHIEITADELVLRRLAEDMPAQEQRFA
                     RRWVRVELAEDPERELVGPLYLRFGGKRTEIASFLGAKERLSFANALKAALINPHF"
     gene            1016..>1706
                     /locus_tag="PKV23_10025"
     CDS             1016..>1706
                     /locus_tag="PKV23_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HQX85814.1"
                     /translation="MEKQTNDYDLVKHTLAFISENWREQPSLETLADQAGLSPTHLQR
                     LFTRWAGLSPKAFLQAVTLDHARGLLRDSASILDASYELGLSGPGRLHDLFVTHEGMS
                     PGIYKAHGRGLSIRYGFHDCPFGRALILITSEGLAGLAFADHGKEKSALADMTSRWPE
                     AAYVEDQQATASYARRIFEAERWQPDQPLKIVFIGTDFEIRVWETILRIPFGKASTYS
                     DIACHIGKPKAA"
BASE COUNT          336 a          514 c          495 g          361 t
ORIGIN      
        1 ccaggcggaa gagatgggta tcaacggcga tggtgggttc tccaaagacg atgttcaaaa
       61 ccacattggc tgtcttgcgc cccacgccgg gaagagcctc cagagcttcg cgttcgccgg
      121 ggactttgcc gccatgcttt tccaccagaa tttttgaaag ggcaatgacg ttcctggcct
      181 tggcgcggta gaggccgata gacttgatgt aatcgcgcaa accctcttcg cccagcgcca
      241 gcattttttc cggggtatcg acggctttga aaagtgcaga tgtggcgcgg ttgacgccca
      301 tatcggtggc ttgcgccgag agcaccaccg ccaccagaaa ggtaaattca ttgacggaat
      361 tgagttcgcc ctttggctcc ggttcaatgt cgtggaagcg ccggaaaatc tcctcgattt
      421 ccggaggctt caagcgcctt gcctttgcca tgaaatccct gctcaaatga tgcgtttgac
      481 aaggtcatag catggacgac acaaagccga aaacctggcg ggccatcctc acgccgcacc
      541 gctccctgtc gcgcaagggt ttcattatcc tcatgttggt catcgccggg ttcaatttca
      601 cccttggcat ggttttttat ctcatcgggg cctggccggt gctgggcttc atggggctcg
      661 atgtggcgct gatcttgtgg gccttccggg ccaatttcgc cgatgcaagg cgggccgagc
      721 atattgaaat caccgccgat gaactggtgc ttcggcgcct tgccgaagac atgccggccc
      781 aggagcagcg ctttgccagg cgctgggtac gggttgaact ggcagaagac ccggagcggg
      841 aactggttgg cccgctctat ttgcgctttg gcggcaaacg cacggaaatc gccagtttcc
      901 taggggcgaa ggagcggctt tccttcgcca acgcactgaa agcggcgctg ataaatcctc
      961 atttctgagc aagaatcggg tggttcccgg cccctggcgg ccctaggttt ggagcatgga
     1021 aaagcaaacg aacgattacg atctggttaa acacacgctg gctttcattt ccgagaactg
     1081 gcgcgagcaa ccctcgcttg aaaccctggc ggaccaggca gggctcagcc ccacacacct
     1141 gcagcgcctg ttcacgcgct gggcgggcct cagccccaag gccttcctgc aggcggtcac
     1201 ccttgaccat gcccgcgggc tgctcaggga ttcggcctcc attctggacg catcctatga
     1261 gctcgggctt tcaggaccag ggcgcctgca tgatctcttt gtgacccatg agggcatgtc
     1321 acccggcatc tacaaggccc atggccgcgg cctcagcatc cgttacggct ttcatgactg
     1381 ccccttcggc cgggcgctga tcctgatcac cagcgaaggc ctcgcaggcc ttgcctttgc
     1441 cgaccatggc aaggagaaat ccgccttggc cgacatgacg tcgcgctggc cggaagccgc
     1501 ttacgtggaa gaccagcagg cgaccgcttc ctatgccagg cgcatttttg aagcggagcg
     1561 ctggcagccg gaccagccct taaagattgt cttcatcggc acggatttcg aaatccgggt
     1621 gtgggaaaca atcctgcgca ttcccttcgg caaggcgtcc acctattcgg atatcgcctg
     1681 ccacatcggt aagccaaaag cggcac
//
LOCUS       DAORVN010000541         1702 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_81711_length_1702_cov_2.075896, whole genome shotgun sequence.
ACCESSION   DAORVN010000541 DAORVN010000000
VERSION     DAORVN010000541.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1702
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81711_length_1702_cov_2.075896"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..505
                     /locus_tag="PKV23_10030"
     CDS             <1..505
                     /locus_tag="PKV23_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008193818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HQX85815.1"
                     /translation="ALDAKVGDGDTGSTIATGARSLKSHLSKLPLADNPSLLASISDL
                     LGKSMGGSSGVLLAIMFAAAGQALKAGVSLPAALQAGLERMKEIGGAKTGDRTMIDAL
                     EPALAALAGGKSLGAAAALARQGADATAYMTRAGAGRSTYVSSANLKGVPDPGALAVA
                     RLLEGLA"
     gene            730..1485
                     /locus_tag="PKV23_10035"
     CDS             730..1485
                     /locus_tag="PKV23_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009735807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
                     /protein_id="HQX85816.1"
                     /translation="MHSHTIAALRASLARPGLEQAAVRLPLGHAAADLCLKGGLERGV
                     LHEVFAGPGHEAAATGFTAGLAARVAARKRLLWIRQDFSALEFGELSATGFLEFGIDP
                     ERLLLLCVADAAGALRAAHDALSCAALGAVVIEIAGRPKILDLMASRRLTLASAQKTV
                     TTFLLRFNAEPQASTAETRWLVRAAASPTQAENWGHPVFEAGLVRNRHGRTGEWVMEW
                     SCDEGLFKETDRGAVVSAPSDRPVAAARQAASR"
     gene            1499..>1702
                     /locus_tag="PKV23_10040"
     CDS             1499..>1702
                     /locus_tag="PKV23_10040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HQX85817.1"
                     /translation="MVVAKVDNALRLSAVDRKATFLGLVIGQPLANARAMLPALTVVA
                     ANEPDDLKLLTRIADWCDRFTPHV"
BASE COUNT          275 a          564 c          529 g          334 t
ORIGIN      
        1 tgcccttgat gccaaggtgg gcgatggcga tacgggctcg accattgcca ccggcgcgcg
       61 cagcctcaag tctcatcttt ccaaattgcc cttggccgat aacccaagcc tactcgcatc
      121 gatcagcgac cttctcggaa aatccatggg cggctcgagc ggcgtgttgc tggccatcat
      181 gtttgccgcc gcagggcagg ccttgaaggc gggcgtttcc ttgcccgccg ccttgcaggc
      241 gggcctggag cgcatgaagg aaatcggcgg cgccaaaacc ggtgaccgca ccatgatcga
      301 tgcgctcgag ccggcccttg ccgcccttgc cggtggaaag tccttgggcg ctgccgcggc
      361 cctggcccgc cagggggccg atgcgaccgc ctacatgacg cgcgccgggg ccggccgctc
      421 aacctatgtg tcgtccgcca atctcaaggg cgttcccgat ccgggcgccc tggccgtggc
      481 gcggctgctg gaaggtctcg cctgaagact gaattaagaa tataaaccct caataggctt
      541 ttaaacgcaa ttagagcgcc ctgcagcggc ttttcgcccg ggggtgctag ggtggtggcc
      601 cgcaagggca gattcctctg ctggcctgtc aggtgcctta ggatcagttt caacttaaag
      661 tattaaattc ctaagattct cgcgcacgac ccgcttgact gtgttcctat tttgttcttt
      721 tctccttcca tgcacagcca caccatagcg gccctccggg cctcccttgc caggccggga
      781 ttggagcaag ccgcggtccg ccttccgctc ggccatgccg cagccgatct ctgcctgaag
      841 ggcggacttg agcgcggcgt attgcatgag gttttcgcag ggcccggcca tgaagcggcg
      901 gcaaccggtt tcaccgcggg cctcgctgcc cgcgtggccg cgcgaaaacg cctgctctgg
      961 atccgccagg atttttccgc cctcgaattc ggcgagcttt cggcaaccgg ctttcttgaa
     1021 ttcggcattg atccggaacg cctgctgctg ctctgcgtgg ccgatgcggc gggcgcactt
     1081 cgcgctgccc atgatgcgct ctcctgcgcc gccctgggtg ctgttgtgat cgagattgcc
     1141 ggccggccga aaattctcga cctcatggcc agccggcggc tgacgctcgc ctcagcgcaa
     1201 aaaaccgtca ccacttttct cctgcgcttc aatgccgagc cccaggccag cacggcggag
     1261 acccgctggc tggtgcgcgc cgccgcctcg ccaacccagg cggaaaactg gggccatccg
     1321 gttttcgaag caggcctcgt ccgcaaccgg catgggcgga cgggagagtg ggtcatggag
     1381 tggagttgcg atgaaggtct cttcaaggaa acggatcgtg gcgctgtggt ttccgcgcct
     1441 tccgaccgac cggttgcagc ggcgcggcaa gcggcaagcc gttgaagcac cgccgctggt
     1501 ggttgtggcg aaagtggaca atgcgttgcg cctctccgcc gtggatcgca aggccacctt
     1561 cctcggcttg gtaatcggcc agcccctggc caatgcgcgc gccatgctgc cggcgctgac
     1621 ggttgtggcg gcaaacgaac ccgatgatct gaaactgctc acccgcatcg ccgactggtg
     1681 cgaccgcttc accccccatg tg
//
LOCUS       DAORVN010000542         1701 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_81767_length_1701_cov_2.351154, whole genome shotgun sequence.
ACCESSION   DAORVN010000542 DAORVN010000000
VERSION     DAORVN010000542.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1701
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81767_length_1701_cov_2.351154"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..256
                     /locus_tag="PKV23_10045"
     CDS             <1..256
                     /locus_tag="PKV23_10045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85818.1"
                     /translation="AVALLLLIAATGSVLFVAEAVAIAANPAFQFKIILLGLAVANVI
                     VNEWALRTRGDGAGLAHLTARLPLLAWLGVAGLGRLIAYV"
     gene            365..1312
                     /locus_tag="PKV23_10050"
     CDS             365..1312
                     /locus_tag="PKV23_10050"
                     /inference="COORDINATES: protein motif:HMM:NF014757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HQX85819.1"
                     /translation="MIPGRLETVAVIGNGIIGHGIAQIFAAAGRQVVMIGRNQDSLDR
                     AMNKIAASLDDFAAHGLIDSASRQASLARIKTSLRLEDAAAAQLVIEAVTEDLPLKLD
                     LFGRLDRICPPPAVLGSSSGQPASALIAKVANPERVIATHFWYPPQLIPLVEVCAGPK
                     TAPDVTRWVCEVLKQAGKEPAVIDKEIPGFIGNRLQFAMLREAWALWASGAASAEAID
                     TVVRNSFGRRVGITGPIESADAGGLYTMYHFGRSLMPHLDTSPEPAAAIAELVAKGAN
                     GIANGQGVYDWTKRDGAELIAARMEELFRWLKADKARGN"
     gene            1317..>1701
                     /locus_tag="PKV23_10055"
     CDS             1317..>1701
                     /locus_tag="PKV23_10055"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015596893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HQX85820.1"
                     /translation="MQAGSRVLTENCSFDPYWWRAAPHKQEPAIAPPRSTDVAIIGSG
                     ITGLVAALHLARGGRQVTVFEAHEPGYGASTRNAGYVGRTLKHGFGELIESHGLPFAT
                     QIYGELMDAFLAVKETVESENIACHY"
BASE COUNT          313 a          552 c          526 g          310 t
ORIGIN      
        1 cgccgtggcc ctgctgctgc tgatcgccgc aaccggctcc gtgctcttcg tggccgaagc
       61 cgtggcgatt gccgccaatc ctgcttttca gttcaagatc atcctgctgg gcctggccgt
      121 cgcgaatgtc atcgtcaatg aatgggcgct gcgaacccga ggtgatggcg ccggcctcgc
      181 ccacctcact gcccgtctgc cgctccttgc ctggcttggc gtggccggcc ttggccgcct
      241 catcgcctat gtgtaactgc gatacctgct gaccggttgc ggcattgcca aggccgcgcg
      301 gttaagccaa cttccatgac agaagtggag acgcggatga acgagacaaa tcaattgggg
      361 tcacatgatt cccggccggc ttgaaacggt tgccgtcatt ggcaacggca tcatcggcca
      421 cggcattgcg cagatttttg cggcagcggg caggcaggtc gtgatgatcg gcaggaacca
      481 ggactcgctg gaccgcgcca tgaacaaaat cgccgccagc ctcgacgatt tcgccgccca
      541 cgggctcatt gatagcgcct cgcggcaggc cagtctcgca agaatcaaaa cctcgctccg
      601 gctcgaagat gccgccgcgg cgcagctcgt cattgaggcg gtcaccgaag acctgccgct
      661 caagctcgat cttttcggca ggctggaccg gatttgcccg ccgcctgcgg tactgggttc
      721 atcaagcggc cagcctgcca gcgccctcat tgccaaggtg gcaaatcccg agcgggtcat
      781 cgcaacccac ttctggtatc cgccgcagct cataccattg gtcgaggtct gtgccggccc
      841 gaaaacagcg cctgatgtta cgcggtgggt ttgcgaagtc ctgaagcagg ccggcaagga
      901 gcccgccgtc atcgacaagg aaattcccgg cttcatcggc aaccgcctgc aattcgccat
      961 gctgcgcgag gcctgggccc tgtgggcctc aggtgcggca agcgccgagg ccatcgacac
     1021 ggtggtgcgc aacagcttcg gccgccgcgt cggcatcacc gggcccattg aatcggcgga
     1081 cgccggcggc ctctacacca tgtatcattt cggcaggtca cttatgcccc atctcgatac
     1141 cagtcccgaa cccgccgccg cgatcgccga actggtggcc aagggcgcca acggcattgc
     1201 caatggccag ggtgtttacg actggacgaa gcgtgatgga gccgaactca ttgcggcgcg
     1261 catggaagaa ctgtttcgct ggctgaaggc ggataaggcc cgggggaatt gacgcgatgc
     1321 aagctggcag ccgggttctg acggaaaact gctcctttga tccctattgg tggcgggccg
     1381 cgccccataa gcaggaacca gcaattgcgc ctccgcgcag caccgatgtg gcgatcattg
     1441 gttccggcat taccggcctc gtggcggcgc tgcacctggc gcgcggtggc agacaggtca
     1501 cggtgttcga ggcccatgag ccgggctatg gcgccagcac ccgcaatgcc ggctacgtgg
     1561 gccgcaccct caagcacggc ttcggcgaac tgatcgaaag ccacggcctg cctttcgcca
     1621 cgcaaatcta cggcgagttg atggacgcct ttctcgccgt gaaggaaacc gtggagagcg
     1681 agaatatcgc ctgccactac a
//
LOCUS       DAORVN010000543         1700 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_81811_length_1700_cov_3.483283, whole genome shotgun sequence.
ACCESSION   DAORVN010000543 DAORVN010000000
VERSION     DAORVN010000543.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1700
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81811_length_1700_cov_3.483283"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..674)
                     /locus_tag="PKV23_10060"
     CDS             complement(<1..674)
                     /locus_tag="PKV23_10060"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HQX85821.1"
                     /translation="MKKTSVAQAFMGREWRNLLAVFLLFLLAGCGGMSKVGDLFSDDP
                     ATPQAPPAVSTSIALLLPLSAPGETQNIARAMQQAAQLAVKDLGSGTLIIKDSGGTAD
                     RARSAAQAALDEGAGIILGPLLSTEVQAVSPVARERNVNVIAFSSVSSVATPGTFLMS
                     FLPDEEVSNVVRYAASKGYRNISLLYPASQYGAAIEQALSRAALAEGSTVVAAKSYAR
                     EAAAVT"
     gene            766..1596
                     /gene="rsmI"
                     /locus_tag="PKV23_10065"
     CDS             766..1596
                     /gene="rsmI"
                     /locus_tag="PKV23_10065"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003612004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HQX85822.1"
                     /translation="MPGGLYVTATPIGHLGDVTLRALATMAAADILLCEDTRTTAKLL
                     ERYGIRTRLMAYHEHNAAKVRPAILRSLGEGQVIALVSDAGMPLVSDPGYRLVADCVA
                     HAIPVTAIPGASAVLTALALSGLPTDRFVFLGFLPTKSGERKKLLNEFMGVKASLIAF
                     ESPHRIVDALGDVADVLGNRRVAVSRELTKLHEEVLRGTASEVQAILGARASVKGEIT
                     LVIAPPEPGAAQASDADIESAIDAALESMPAAKAAADIAKRFDLAKKDIYARILARRK
                     "
BASE COUNT          314 a          533 c          554 g          299 t
ORIGIN      
        1 tgggtcacgg cggcggcctc gcgggcatag ctttttgctg ccacgacggt gctgccttcg
       61 gctagcgccg cgcgggaaag cgcctgctcg atggccgcgc catattggga ggcgggataa
      121 agcagcgaga tattgcggta acccttgctt gcggcatagc gcaccacgtt ggagacttcc
      181 tcatcgggca ggaagctcat caggaaagtg ccgggggttg ccacggagct gaccgacgaa
      241 aaggcgatga cattgacatt gcgttcacgc gccacggggg ataccgcctg cacctcggtg
      301 gagagcagcg gccccagaat gatccctgcc ccttcatcga gggcggcttg cgcggcgctg
      361 cgggcccggt cggcggtgcc gccggaatcc ttgatgatca acgtgccgct tcccaagtcc
      421 ttgacggcga gctgggctgc ctgctgcatg gcgcgggcga tgttctgggt ctcgccgggg
      481 gccgaaagcg gcaggagcag cgcaatgctg gtgctgaccg cagggggcgc ctgcggcgtt
      541 gcgggatcat cgctgaacag gtcacccact ttcgacatgc cgccgcagcc ggcaaggaga
      601 aacagcagga aaacggcgag cagatttcgc cactcccgcc ccatgaaggc ttgcgctaca
      661 ctcgtcttct tcacatcgcc accccaacgg taatcaatga cgccacaaac tccatccacc
      721 tcccgacgct atagcatagg tgctggccaa ttcgaggcgg aaaaattgcc gggcggactt
      781 tatgtgacgg caacccccat cggccatctc ggcgatgtca cgctcagggc ccttgccacc
      841 atggctgccg ctgacatcct gctctgcgag gacacccgca ccacggccaa actgctggaa
      901 cgctacggca tccgcaccag gctcatggcc tatcatgagc acaatgccgc aaaggttcgg
      961 cccgccatcc tgcggtcttt gggcgaaggg caggtcattg ccctggtgag cgacgcgggc
     1021 atgccgctgg tctccgatcc aggctaccgg ctggtggcgg actgcgtggc ccacgccatt
     1081 ccggtcaccg cgatccccgg ggcctcagcg gtgctcacgg cgctggcgct ttccggcctg
     1141 cccacggacc gcttcgtttt tctcggcttt cttcccacca aatcgggcga gcgaaaaaaa
     1201 ctgctcaacg agttcatggg ggtgaaggca agcctcatcg cctttgaaag cccgcatagg
     1261 attgtcgatg cgctgggcga tgtggctgat gtgctgggca atcgccgggt tgcggtgtcg
     1321 cgcgagctca ccaagctgca cgaggaagtg ctgcgcggaa ccgcaagcga ggtgcaggcg
     1381 attctcggcg cgcgcgcctc ggtgaagggc gagatcaccc tggtgattgc gccgccggaa
     1441 ccgggtgcgg cacaggcgag cgatgcagat attgaatcgg ccattgatgc ggcgcttgaa
     1501 agcatgcccg ccgccaaagc cgcggcggat atcgccaaac gctttgatct ggccaagaag
     1561 gatatctatg cccgcatcct ggcgcgccgg aaatgagccg gtcgcgggaa aaagcggaga
     1621 agcgcggcca tgtcgccgag ttcgtggcgc tgatgtatct ccgcctgaag ggttaccggc
     1681 tgctggccca gcgtttcaag
//
LOCUS       DAORVN010000544         1693 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_82407_length_1693_cov_4.678877, whole genome shotgun sequence.
ACCESSION   DAORVN010000544 DAORVN010000000
VERSION     DAORVN010000544.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1693
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82407_length_1693_cov_4.678877"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            66..>1693
                     /locus_tag="PKV23_10070"
     CDS             66..>1693
                     /locus_tag="PKV23_10070"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQX85823.1"
                     /translation="MTASTNLFAPDEDVSRPVTNFGPDFPFAFDDWIKHPAGLGSVPA
                     HRHGEEVAIIGAGMAGMTAAFELLKMGLKPVVYEASRIGGRLRSQVFEGAEGVIAELG
                     GMRFPESSTAFYHYVNMLGLKSKPFPNPLSPATPSTVIDLEGKTLYVEKVADLPPMFR
                     EIGTAWAKALEEGAGLSGLRQAIRERDVPALKEIWDRLIPIWDDRSFYDFVATSYAFA
                     DRPFQYREVFGQVGFGTGGWDSDYPNSMLEILRVVATDCDENQNYIVGGVEQVPRGLW
                     KTSPPNMVHWPKGTTLESLHRGAPRPGVTRIDRAYGDKIAVTDRWGDTKLYDAVLVAL
                     QSWLLTTYMKCEESLFSPKMWMALDRTRYMQSSKTFVMTDRPFWKDKDPHTGRDLMSM
                     TLTDRLTRGTYLFDNGDDKPGVICLTYSWMSDAMKMLPLPVEKRVTLALDALKKIYPD
                     LALEKHIIGDPITVSWESDPNFMGAFKGALPGHYRYNHRMYCHFMQDKMPPAERGIFI
                     AGDDVSWTPGWVEGAVQTALNAVWGIMHHLGGESP"
BASE COUNT          331 a          479 c          548 g          335 t
ORIGIN      
        1 gtgcaatctc cccgtggccg ggcggttttt tatgtggcaa gctcgtaaaa acggagtccg
       61 cccacatgac tgcctcaacc aatctatttg ctcccgacga ggatgtctcg cggccggtca
      121 cgaacttcgg cccggatttc cccttcgcct ttgatgactg gatcaagcac ccggcgggcc
      181 tcggctcggt gccggcgcac cgccatggcg aagaggtggc catcatcggc gcggggatgg
      241 ccggcatgac ggcagccttt gagcttctga agatggggct gaagccggtg gtctatgagg
      301 cctcgcgcat tggcgggcgg ctgcgttcgc aggtctttga aggggcggaa ggggtcatcg
      361 cggaactggg cggcatgcgc tttccggaat cgtccactgc tttctatcat tatgtgaaca
      421 tgctcgggtt gaagtccaag ccatttccaa atccgctgtc gccggcaacc ccttcaacgg
      481 tgatcgatct cgagggcaag acgctttacg tggaaaaggt tgccgatctt ccgccgatgt
      541 tcagggaaat cggcacggcc tgggccaagg cgcttgaaga aggagccggg ctttccggcc
      601 tgcggcaggc gatccgcgag cgcgatgtgc cggcgctgaa ggaaatctgg gaccggctca
      661 ttccgatctg ggacgaccgc agcttctatg atttcgtggc gacgtcctat gcctttgccg
      721 acaggccgtt tcaataccgc gaggtttttg gccaggtggg tttcggcacc ggaggctggg
      781 attcggacta tcccaattcc atgctggaaa tcctgcgggt tgtggccacg gactgcgacg
      841 agaaccagaa ctacattgtg ggcggagttg agcaggtgcc gcggggattg tggaagacgt
      901 cgcctcccaa catggtgcat tggcccaagg gcacgactct cgaatccctg catcgcggcg
      961 caccgcgccc gggcgtcacc cgcattgacc gggcctatgg cgacaagatc gcggtgaccg
     1021 accgctgggg cgacacaaaa ctctatgatg cagtgctggt ggcgctgcag agctggctgc
     1081 tgaccaccta catgaaatgc gaggagagcc tgttttcgcc gaagatgtgg atggcgctgg
     1141 accgcacgcg ctacatgcaa tcgtcaaaaa ccttcgtgat gacggaccgg cccttctgga
     1201 aggacaagga cccgcataca gggcgtgatc tgatgagcat gacgctcacc gaccggctga
     1261 cgcgcggcac ctatctgttt gacaatggcg acgacaaacc gggcgtgatc tgccttacct
     1321 attcgtggat gagcgatgcg atgaagatgc tgcccttgcc ggtggagaag cgggtgacgc
     1381 tggcgctgga cgcgctgaag aaaatctatc cggatctcgc attggaaaaa cacatcatcg
     1441 gcgatcccat cacggtgtca tgggagtcgg accccaattt catgggcgcc ttcaaggggg
     1501 cgctgcccgg ccactaccgc tataaccacc ggatgtattg ccatttcatg caggataaaa
     1561 tgccgccggc ggaacgcggc atcttcattg cgggcgatga tgtgtcgtgg acgccgggct
     1621 gggtggaagg agcggtgcaa acggcgctca acgcggtgtg gggcatcatg catcacctgg
     1681 gcggcgaaag ccc
//
LOCUS       DAORVN010000545         1693 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_82463_length_1693_cov_2.336386, whole genome shotgun sequence.
ACCESSION   DAORVN010000545 DAORVN010000000
VERSION     DAORVN010000545.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1693
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82463_length_1693_cov_2.336386"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1102
                     /locus_tag="PKV23_10075"
     CDS             <1..1102
                     /locus_tag="PKV23_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020180083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HQX85824.1"
                     /translation="KAQCEALGCKVQVDSVGNMFATRAGKRKDLLPIALGSHLDTQPT
                     GGKFDGVLGVLGALEALRTLVDMGYESNAPLMIVNWTNEEGSRFAPAMLCSGVYAGVF
                     TPEFAYSREDRDGIKFGDELKRIGYLGSEPVGSHKFQAMFELHIEQGPILEAEVKMIG
                     VVTGVQGMRWYEVTVHGQESHTGATPMLLRKNALLGASRMIEAIHQVGLSHLPGVASV
                     GLIENRPNSRNVVPGEVFFTVDLRHPDEAVLDKMEAAFRAALGPIAEAEKLTYEEKCI
                     WKSPAVKFNAELIDCVRKGGSQAGYKMREMASGAGHDAAYIARVAPTTMIFVPCLGGI
                     SHNEAESTTFDECGAGSQVLLNAVLEYDQKFA"
     gene            1083..>1693
                     /locus_tag="PKV23_10080"
     CDS             1083..>1693
                     /locus_tag="PKV23_10080"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HQX85825.1"
                     /translation="MTRNSPELSARAREIALELTSWPSVTGTQGEAAFAQRLQDYLRL
                     KGFDEGISLSVDAGPNPRGNIAVLKRGRSARTVVLTGHFDTVSYDDYGALTDLALDAE
                     ALTISITSRLRKSGENKLAMRDFESGEFLAGRGLLDMKAGLAAGLAAMEAYAGDASIL
                     FLAVCDEEETSAGARAAAPALKSLEKELGLEIKLVINLDAISD"
BASE COUNT          339 a          434 c          566 g          354 t
ORIGIN      
        1 caaggcgcaa tgcgaggcgc tgggctgcaa agtgcaggtt gacagcgtgg gcaatatgtt
       61 tgcgacgcgg gcgggaaagc ggaaggatct gctgccgata gcacttggct cgcatctcga
      121 tacacagcca acgggcggca agtttgacgg tgttctgggt gtgctcgggg ctttggaggc
      181 gctgcgcacg ctggtggaca tgggctatga aagcaatgcg ccgctgatga tcgtgaactg
      241 gaccaatgag gagggttcgc gattcgcgcc ggccatgctg tgttcggggg tttacgctgg
      301 cgtgttcacg ccggagtttg cctattcgcg cgaggaccgg gacggcatca agtttggtga
      361 tgaactgaag cgcatcggtt atctgggttc cgagcctgtc ggctcccaca aattccaagc
      421 gatgtttgag cttcatatcg agcaggggcc gatccttgag gcggaagtaa aaatgatcgg
      481 cgtcgtcacc ggcgtgcagg gcatgcgctg gtatgaggtg acggtgcacg gccaggaatc
      541 ccatacgggg gcgaccccga tgctgctgcg caagaatgcg ctgctcggcg catcgcggat
      601 gatcgaagcc attcaccagg tggggctttc gcatttgccg ggcgttgcat cggttggcct
      661 gatcgaaaac cggccgaaca gccgcaatgt ggtgccgggg gaagtattct tcacggttga
      721 tttgcgccat cccgatgaag cggtgctgga caagatggag gcggctttcc gcgcggcgtt
      781 ggggccgatt gccgaagcgg agaagctcac ctacgaagag aaatgcatct ggaaatcgcc
      841 ggcggtgaag ttcaatgcgg agcttattga ctgcgtgcgc aagggcggtt cccaggcggg
      901 atataaaatg cgcgaaatgg catcgggtgc cgggcatgat gcggcctata tcgccagggt
      961 ggcgcccacc acgatgattt ttgtaccctg cctgggcggc atatcgcaca atgaggcgga
     1021 gtccaccacc tttgatgaat gtggtgcggg ttcccaggtg cttctgaacg cagttcttga
     1081 atatgaccag aaattcgcct gagctgtctg cccgggcgcg ggagattgct ctggaactca
     1141 cttcatggcc ttcggtaacg ggaacgcagg gagaggctgc atttgcgcag cggttacagg
     1201 attatctccg cctaaagggg tttgatgaag gcatatcgct ttccgttgac gcagggccaa
     1261 acccgcgcgg taatatcgcg gttctgaagc gcggccgttc tgcccgcaca gtcgttctga
     1321 ccggccattt cgatactgtc tcctacgatg actacggtgc gctcaccgac ttggcgctcg
     1381 atgcggaagc gcttaccata tccatcacta gcaggctgcg gaaaagcggc gaaaacaaac
     1441 tcgccatgcg ggattttgag agcggagagt ttctcgcggg gcgcgggctt ctcgacatga
     1501 aggccggatt ggctgcgggg cttgccgcca tggaggcgta tgcaggggat gcctcaattc
     1561 tgttcctagc ggtttgcgat gaagaggaaa cgtcggctgg cgcccgggcc gcggcacctg
     1621 ccttgaaaag cctggaaaag gagctcggcc ttgaaatcaa actggtcatc aacctcgatg
     1681 ccatttcgga tca
//
LOCUS       DAORVN010000546         1690 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_82681_length_1690_cov_2.951682, whole genome shotgun sequence.
ACCESSION   DAORVN010000546 DAORVN010000000
VERSION     DAORVN010000546.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1690
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82681_length_1690_cov_2.951682"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..503)
                     /gene="rsmD"
                     /locus_tag="PKV23_10085"
     CDS             complement(<1..503)
                     /gene="rsmD"
                     /locus_tag="PKV23_10085"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009758897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HQX85826.1"
                     /translation="MRIVGGKFKGLHLATPSDQATRPTSDRVREAVFNILAHGIDGLE
                     LDGARVLDLFAGTGALGFEALSRGARHCQFVDESAEARGLVRTNADKLGVIGQCKIWR
                     RDATDLGPCAPLPRFDLVFADPPYGKGLGTKALQALVQGEWINGGGIVVLEENEKAEV
                     PEVAGLTL"
     gene            complement(478..1239)
                     /locus_tag="PKV23_10090"
     CDS             complement(478..1239)
                     /locus_tag="PKV23_10090"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HQX85827.1"
                     /translation="MSEFEGERIAKVIARAGVCSRREAEQLILDGHVTLNHKVLKTAA
                     INVTDADQIRINGKPLPRKEESKLWRYHKPEGLVVSHKDPQGRPSVFDKLAQQLPRVV
                     SIGRLDINTEGLLLLTNDGEIERLLELPSSGWIRRYRVRAYGKMDDAGLAKLGQGVEI
                     DGVRYGPVEAKVESSQGDNVWMTVAIREGKNREVKKICEYLGLQVNRLIRTSFGPFQL
                     GELKRGGIEEIPQKFWQEQLGGKFRKSDADRRRKV"
     gene            1277..>1690
                     /locus_tag="PKV23_10095"
     CDS             1277..>1690
                     /locus_tag="PKV23_10095"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011995559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HQX85828.1"
                     /translation="MTQKLPEPMLRAFAEAEKAAQRGEVPVGAVITDKLGNILAVAGN
                     RTLVDHDPTAHAEMLVIREAASKLDSERLIDCDLYVTLEPCAMCAAAISFARIRRLYF
                     AAADEKMGAVEHGPVFFAQSTCHHKPEVYGGIGGQR"
BASE COUNT          303 a          588 c          466 g          333 t
ORIGIN      
        1 agtgtcagcc cggcaacctc gggcacttcc gctttctcat tctcttccag caccacaatc
       61 ccgcccccat tgatccactc gccctggaca agcgcctgca aggccttcgt cccaagcccc
      121 ttgccatagg gcgggtcagc gaaaaccaga tcaaaccggg gcagcggcgc gcaggggcct
      181 aggtccgtgg cgtcccgccg ccagatcttg cactggccga taacgccaag cttgtccgca
      241 ttggtccgca ccagcccccg cgcctcggcg ctctcatcga cgaactggca atggcgtgcc
      301 cctcgcgaca gggcttcaaa gcccaacgcc ccggtgccgg caaacaggtc cagcacccgc
      361 gccccgtcca gttcaagtcc gtctatgcca tgggccagga tgttgaacac cgcttcgcgc
      421 acccggtccg aggtgggccg cgtcgcctga tccgaaggtg ttgcgagatg aagcccctta
      481 aactttccgc cgacgatccg catcactctt cctgaatttt ccgcccaatt gctcctgcca
      541 gaatttttgc ggaatttctt ctattccccc gcgcttcaac tcgccgagct ggaagggccc
      601 gaaactggtg cggatcaggc ggttcacctg caggccgaga tattcgcaga ttttcttcac
      661 ctcgcggttc ttgccctcgc ggatggccac cgtcatccag acattgtcgc cctgcgatga
      721 ttcgaccttg gcttccaccg ggccatagcg cacgccgtcg atctccacgc cctggccgag
      781 cttggccagc cccgcatcat ccatcttgcc ataggctcgc acccggtagc gccggatcca
      841 gccggacgac ggcaattcca gcagccgttc gatctcgccg tcattggtga gcagcagcag
      901 gccctccgta ttgatgtcga gccggccgat ggacacgacg cgcggcagct gctgtgccag
      961 tttgtcaaat accgagggcc gcccctgtgg gtccttgtgg ctcaccacca ggccctccgg
     1021 cttgtgatag cgccagagct tcgactcttc cttgcgcggc aagggctttc cgttgatgcg
     1081 gatctggtcg gcatcggtca cgttgatggc ggcggtcttg agaaccttgt ggttgagcgt
     1141 cacatggccg tcgaggatca gctgctccgc ctcgcgccgc gagcagacgc cagcccgggc
     1201 aatcaccttg gcgatccgct cgccttcaaa ttcgctcatc ttgacctcga cttcacttta
     1261 gggctcatct cgcacgatga cacagaaact gccagagccc atgttgcggg ccttcgccga
     1321 agccgaaaag gcagcccagc ggggcgaagt gcccgtgggc gccgtgatca ccgacaaatt
     1381 gggtaatatt ttggcggtcg ccggaaaccg cacccttgtg gaccacgacc ccaccgccca
     1441 tgccgagatg cttgtcatcc gggaagcggc cagcaaattg gattccgaac gcctaattga
     1501 ttgcgatctc tacgtgacgc tcgagccctg cgccatgtgc gccgccgcca tttccttcgc
     1561 ccgcataagg cggctctatt tcgccgcggc ggacgaaaaa atgggcgcag tcgaacacgg
     1621 cccggttttc tttgctcaat ccacctgcca ccacaaacct gaagtctacg gcggcatcgg
     1681 cgggcaaagg
//
LOCUS       DAORVN010000547         1688 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_82845_length_1688_cov_3.218004, whole genome shotgun sequence.
ACCESSION   DAORVN010000547 DAORVN010000000
VERSION     DAORVN010000547.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1688
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82845_length_1688_cov_3.218004"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1515)
                     /locus_tag="PKV23_10100"
     CDS             complement(<1..1515)
                     /locus_tag="PKV23_10100"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF028500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HQX85829.1"
                     /translation="MRPSVLNPLFASASGIKGIGQKLDKVLAQFLRGDATAPTRIVDL
                     LFHLPSGVVDRRFRPKIADLPREGIVTVEVKVGRHKPPPSFNKKLPYRVEVSDETGSL
                     TLVFFYAFVDSLKRGLPEGETRFISGAISWYGEEPQIAHPDHILTAEDFSHMPMIEPV
                     YPLTAGLSPKILGRAIRAAVERLPDLAEWQDAAWKKKHGWPDFSTALKALHSPQNSEV
                     PSISSPARLRLAYDELLANQLALSLVRGHMKRAAGRSFAGDGTRQAKIIGALPYKLTG
                     GQAFANAEIHADMTSPSRMLRLLQGDVGSGKTIVALLALATAVECGAQGTFMVPTEIL
                     ARQHLASLTPLCAAAGIAIAVLTGREKGKQREDILERLAAGRIDILIGTHALFQEGVE
                     FRNLGLVVIDEQHRFGVHQRLALQAKAGSATDLLVMTATPIPRTLALTVYGDMDVSKL
                     TEKPAGRLPIDTRVLPLERLDEVLAGLGRSIKSGQRAYWVCPLVEGSELVDLAAA"
     gene            1577..>1688
                     /locus_tag="PKV23_10105"
     CDS             1577..>1688
                     /locus_tag="PKV23_10105"
                     /inference="COORDINATES: protein motif:HMM:NF015870.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HQX85830.1"
                     /translation="MTYPAEPIELRRKRLLWRSSHRGIREMDLLMGGFARA"
BASE COUNT          327 a          533 c          502 g          326 t
ORIGIN      
        1 cgccgccgct aggtcgacga gctcgctgcc ttccaccagc gggcagaccc aatatgcccg
       61 ttggccgctt ttgatgctgc gccccagccc cgccaagacc tcatccaggc gctcgagcgg
      121 cagcacgcgc gtgtcaatgg gcaggcgccc ggcgggcttt tcggtgagct tggatacgtc
      181 catgtcgcca taaaccgtca gcgccagcgt gcggggaatg ggagtggccg tcatgaccag
      241 aaggtccgtg gcgcttccag ccttcgcctg cagcgccagc cgctggtgca ccccgaagcg
      301 atgctgctcg tcgatcacga caagtccaag gttcctgaat tcgacccctt cctgaaacag
      361 cgcatgggtg ccgatcagaa tatcgatcct gcctgctgca agtcgctcca gaatatcttc
      421 ccgctgcttt cccttttcgc ggcccgtgag aactgcaatg gcgatcccgg ccgccgcgca
      481 aagcggtgtg agggatgcaa ggtgttgccg cgccagaatt tcagtgggaa ccatgaaggt
      541 gccttgcgcc ccgcattcaa ccgcagttgc aagggcaagc aaggccacga tggtctttcc
      601 cgatccaaca tcgccctgca gcagccgcag catgcgcgat ggtgaggtca tgtcggcgtg
      661 gatctccgca ttggcaaatg cctgtccgcc ggtcagcttg tagggcaggg cgccaatgat
      721 cttggcctgc cgtgtgccat cgcccgcaaa gctccgcccc gccgcccgct tcatgtgccc
      781 gcgcaccagg ctcaacgcca attgatttgc cagcagctcg tcataggcca gtcttaaacg
      841 tgcgggtgaa ctgatcgatg gcacttcgct gttctgcggg ctgtgcagcg ccttgagcgc
      901 cgtcgaaaaa tccggccagc catgtttctt tttccaagcc gcatcctgcc actccgccag
      961 gtccggcaat cgctccacag ccgcacgaat tgcccgcccg aggattttgg gtgagagccc
     1021 tgccgtaagc ggatacaccg gttcgatcat cggcatgtgg ctgaaatctt ccgccgtcag
     1081 gatatggtcc gggtgtgcaa tctgcggttc ctcgccgtac cagctgatcg cgccggaaat
     1141 aaaccgcgtt tcgccctccg gcaggccgcg cttaaggctg tccacaaagg catagaaaaa
     1201 caccagggtc agcgaaccgg tctcgtctga gacctcgacc cggtagggca atttcttgtt
     1261 gaaggacggc ggtggcttgt ggcgtcccac cttcacctcg acggtgacga tgccctccct
     1321 gggcagatca gcgattttcg ggcgaaagcg ccggtcaacc acgccgctcg gcaaatgaaa
     1381 cagcagatcg acaatccggg tgggagccgt agcatcgccg cgcaggaatt gcgccagaac
     1441 cttgtcaagc ttctggccaa tgcccttgat gcccgaggcg cttgcaaaca ggggattgag
     1501 aacagaagga cgcatgctgc cattttgttg gctaaggtgt tgcagcgcaa gtggggaagc
     1561 ggctaatcag gcggcaatga catacccagc tgagcccatt gaattgcgcc ggaaacgtct
     1621 tctatggcgc agcagtcacc gtggcatcag ggaaatggac ctgctgatgg gaggcttcgc
     1681 cagagccc
//
LOCUS       DAORVN010000548         1687 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_82965_length_1687_cov_2.475490, whole genome shotgun sequence.
ACCESSION   DAORVN010000548 DAORVN010000000
VERSION     DAORVN010000548.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1687
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82965_length_1687_cov_2.475490"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..325)
                     /gene="bamD"
                     /locus_tag="PKV23_10110"
     CDS             complement(<1..325)
                     /gene="bamD"
                     /locus_tag="PKV23_10110"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HQX85831.1"
                     /translation="MQASRLLGIAAVALGLAGCSTASVIKDVFGNDSTPAASTSVGLT
                     GGALSPTDEAPGRSDPVAELYNGGLEKLKSGSYKSAAKSFAEVERQHPYSKWATKALL
                     MQAFAY"
     gene            complement(485..>1687)
                     /locus_tag="PKV23_10115"
     CDS             complement(485..>1687)
                     /locus_tag="PKV23_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020094132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HQX85832.1"
                     /translation="TGAAPVIARAAKEQGILTVGVVTKPFHFEGVRRLQTAERGIEQL
                     AQYVDTLIVIPNQNLFRVANEKTTFAAAFAMADQVLYSGVASITELMTKEGLINLDFA
                     DVRAIMSEMGRAMMGTGEATGEKRAIEAAEAAISNPLLDDVSMRGARGLLISISGGPD
                     LTLYEVDEAATRIREEVDPEANIILGATFDDSLEGTMRVSVVATGLAQELLTSSDPAE
                     AEGEMPEASGTSFGYTGKQQTIIPKVVKPAPMAARANPVANKAPAPPPQPAAEVAIED
                     DHETQVAAKASAKGRGQRMPAIEEFPPIAQKQIAAQQNRIEHIAEHAARKKKGLFERL
                     ANVGLGRRDDPAPVTREPVMAVRSEPKVQVTRAPARPAAPEPQTYIDPNLDDDQLTIP
                     AFLRRQAN"
BASE COUNT          308 a          508 c          511 g          360 t
ORIGIN      
        1 aataggcgaa ggcctgcatc agcagggcct tggtggccca ctttgaatag ggatgctgcc
       61 gctcaacttc ggcaaagctt ttggccgccg acttgtagga gccggatttg agcttttcga
      121 ggccgccatt atagagttcc gcaaccggat cgctccgtcc gggagcctca tcagtggggc
      181 tcaatgctcc cccggtcagc cccacgctgg tgctcgccgc gggcgtcgaa tcattgccga
      241 aaacatcctt tatgacgctc gcggtcgaac acccggcaag ccccagggca actgccgcaa
      301 tgcccagcaa acgcgaggcc tgcataaggt gttttccgga aagaaccatt cgcaactaac
      361 tctcatatct gattggcagc atattctagc ccatcgcgcc gggaacatga agcagatagc
      421 gttagaagca cgaaacccgg cggaaatgtg accgccgggg ttctaaaatg cgtccgttaa
      481 tcaatcagtt ggcctggcgc cgcaggaagg ccggaatggt cagttgatca tcgtccagat
      541 tggggtcgat ataggtctgg ggttccggcg cggccggccg tgccggcgcc cgggtcactt
      601 gcacctttgg ctcggaacgc accgccatga ccggttcgcg cgtaacgggg gcgggatcgt
      661 ccctgcggcc caggccgaca tttgccaggc gctcgaacag gcccttcttc ttgcgcgcgg
      721 catgctcggc aatatgctcg atgcggtttt gctgagccgc tatctgcttc tgcgcaatcg
      781 gcggaaactc ttcaatggcg ggcatgcgtt ggccgcgccc cttggctgaa gccttggccg
      841 caacctgtgt ctcatgatcg tcttcgatgg cgacttctgc ggcaggctgc gggggcgggg
      901 ctggcgcttt gttggcgaca ggatttgccc gggccgccat tggcgccggt ttcacgactt
      961 tcggaataat ggtctgctgt tttccggtgt agccgaagga tgtgccggat gcttccggca
     1021 tttcgccttc cgcctcagcc ggatcggagc ttgtcagaag ctcctgggca aggcccgttg
     1081 caactactga aactcgcatc gtgccttcca gagaatcatc aaaggtggca cccagaatga
     1141 tattggcctc cggatccact tcttcccgga tgcgcgttgc cgcttcatcc acttcataga
     1201 gagtcaagtc tggtccgccc gaaattgaaa tcagaaggcc gcgcgccccg cgcatcgaca
     1261 catcatcaag caagggattg gagatcgcgg cttcggccgc ttcaatggcg cgtttttcgc
     1321 cggtcgcctc gccggtgccc atcatggccc ggcccatttc gctcatgatg gcgcggacgt
     1381 cggcgaaatc gagattgatc aggccttcct tggtcatcag ttcggtgatc gaggcaacgc
     1441 ccgagtaaag cacctgatcg gccatggcaa aggctgcggc aaaggtcgtc ttttcgttgg
     1501 cgacacggaa caggttctgg ttcgggatga cgatcagcgt atcaacatat tgcgcgagct
     1561 gttcgatgcc gcgctcggcg gtctgcaggc gccgcacccc ttcgaaatgg aacggcttgg
     1621 tgaccacgcc gacggtcagg atgccctgtt cctttgcggc gcgcgctatg accggcgccg
     1681 cacctgt
//
LOCUS       DAORVN010000549         1684 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_83236_length_1684_cov_2.138735, whole genome shotgun sequence.
ACCESSION   DAORVN010000549 DAORVN010000000
VERSION     DAORVN010000549.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1684)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1684)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1684
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83236_length_1684_cov_2.138735"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..906)
                     /locus_tag="PKV23_10120"
     CDS             complement(<1..906)
                     /locus_tag="PKV23_10120"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004160.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HQX85833.1"
                     /translation="MRLLGALEPIPLEFHHAVVAIGNFDGLHRGHQELLLIAKAEAER
                     LGKSWGIVTFEPHPRSFFKPDEPVFRLTPLPLKVRLAAALGASFVLSLNFDKALSMLE
                     PQDFIQQYLVEKMKAAHVVTGYDFHFGRGRKGNPAILKEFGQQRGFDVTVVDQVADED
                     DSHSPFSSSSIRSALRRGHMAAAARELGYYWTVMGKVVKGDHRGRAIGFPTANIVLEE
                     GAEPIRGIFTVRVRDGIKKDSTAWGGAGYFGNRPTFETNRTFLEVHLFDMDEDLYGRT
                     LLVEFIDLIRPDQKFNSVAELVEQMT"
     gene            complement(918..1379)
                     /locus_tag="PKV23_10125"
     CDS             complement(918..1379)
                     /locus_tag="PKV23_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HQX85834.1"
                     /translation="MDERDSPNMLDNMPRGTICIEDIEIGMSRHLMKVITDRDIELFA
                     EVSTDRNPVHLDDSYAQDTIFEGRIAHGMLTASFISTVLGTRLPGPGCIYLSQNLRFL
                     APVRIGDTVKALARVASLDMAKRRARFACHCEVNGKIVVEGEATVFVPSRG"
     gene            complement(1476..>1684)
                     /locus_tag="PKV23_10130"
     CDS             complement(1476..>1684)
                     /locus_tag="PKV23_10130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR01459 family HAD-type hydrolase"
                     /protein_id="HQX85835.1"
                     /translation="DPQILCIGDGISTDIQGGIGEGLDTLFISGGLEAERFGADAENP
                     DQGLLDDWLDSKELSPTFAMGRLR"
BASE COUNT          367 a          481 c          502 g          334 t
ORIGIN      
        1 cgtcatctgc tcaaccagtt ccgcaaccga attgaatttc tggtcgggac ggatgagatc
       61 gataaattcc accagcaggg tccgcccata cagatcctca tccatgtcga aaagatggac
      121 ttcgagaaag gtccggtttg tttcaaaggt cgggcgattg ccgaaatatc cggcaccgcc
      181 ccatgcagtg gaatctttct ttatgccgtc acgcacgcgc accgtgaaga tgccgcgaat
      241 gggttcggcg ccttcttcga gtacaatatt cgcggtggga aagccaatgg cgcggccgcg
      301 gtgatcgccc ttcacaacct tgcccatcac agtccagtag tagccgagct cgcgggcggc
      361 ggcggccatg tggccgcggc gcagggcgga ccggatagaa ctggatgaga atggtgaatg
      421 gctgtcatcc tcgtcggcca cctggtccac aacggtgacg tcaaatccac gctgctgtcc
      481 aaattccttg agaatcgcgg gattgccctt gcggccccgg ccaaaatgaa aatcatatcc
      541 ggtgacgaca tgggcagcct tcattttttc aaccaagtat tgctggatga aatcctgcgg
      601 ctccagcatg gacagggcct tgtcgaaatt caggctgagg acaaatgaag cgccaagggc
      661 ggcggccagg cggactttca agggaagcgg cgtcaggcgg aataccggtt catcgggttt
      721 gaaaaaactt ctggggtggg gctcgaaggt tacgatgccc caggattttc ccagccgttc
      781 ggcctccgct ttggcgatga gaagcagctc ctggtggccg cgatgcaggc catcaaagtt
      841 tccgatcgcc accacggcat gatgaaattc caggggaatg ggctcaaggg ccccaagcaa
      901 acgcattcat tctcacttca gccacgcgac ggcacaaata ccgttgcttc gccttcgacc
      961 acaatcttgc cattcacttc gcaatggcag gcaaaccggg cgcggcgctt agccatatca
     1021 agacttgcca cgcgggccaa ggccttcacc gtatcgccga tccggacggg cgccagaaac
     1081 cgaaggtttt gcgacagata aatgcagccg gggccgggaa gcctggtgcc gagaacggtg
     1141 gatatgaagc tggcggtcag catgccgtgg gctatgcggc cctcgaagat ggtgtcctgg
     1201 gcatagctgt cgtccagatg gaccgggttc cggtcggtcg agacttcagc gaacagctcg
     1261 atatcccggt cggtgatgac cttcatcaga tggcgggaca tgccgatttc gatgtcctcg
     1321 atgcagatcg tgccgcgggg catgttgtcc agcatgttgg gcgaatccct ttcatccatg
     1381 cgggaaacgc tagcacatgc tgcggcgcag aacaatcggc tgcgcaataa aagttcactg
     1441 cggcgaaatg cggtgaacga aagttactca gcggatcagc gcaaccggcc catggcaaat
     1501 gtcggcgaca gttccttgct gtcgagccaa tcgtccagaa ggccttggtc cgggttctcg
     1561 gcgtcggcgc cgaaccgctc ggcttcgagc ccgcccgaga tgaagagggt atccagcccc
     1621 tcgccgatac caccttgaat atccgtggaa attccgtcgc cgatgcacag gatctgcggg
     1681 tcgg
//
LOCUS       DAORVN010000550         1683 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_83289_length_1683_cov_2.925676, whole genome shotgun sequence.
ACCESSION   DAORVN010000550 DAORVN010000000
VERSION     DAORVN010000550.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1683
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83289_length_1683_cov_2.925676"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="PKV23_10135"
     CDS             complement(<1..184)
                     /locus_tag="PKV23_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018688747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylhomoserine sulfhydrylase"
                     /protein_id="HQX85836.1"
                     /translation="MAKYTKRQNPKGWGDATKLVRGGLDRSLHGETSEAIYLTSGYVY
                     DDPETAERRFAGEEDGY"
     gene            complement(184..585)
                     /locus_tag="PKV23_10140"
     CDS             complement(184..585)
                     /locus_tag="PKV23_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008550732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HQX85837.1"
                     /translation="MHRGNISPAEAHQRLQGNSSAVLVDVRTQPEWTFVGVPAVERLV
                     RLAWQVFPAMQVNAKFVEEIEQMGLPKDAEILCICRSGARSSSAASALTDAGYTNCWN
                     VAQGFEGDKDANGHRGTVNGWKVAGLPWVQS"
     gene            complement(671..976)
                     /locus_tag="PKV23_10145"
     CDS             complement(671..976)
                     /locus_tag="PKV23_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012708943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF718 domain-containing protein"
                     /protein_id="HQX85838.1"
                     /translation="MTAYNVVRFKVKPGQEQKFIELHKSLPDLDGMKDGALIKTGERA
                     YCFVGKWDDYESIVEARPQMVGILDGFRAMLEDLGGGLGLTDPVSGPAIVKYPSHKH"
     gene            1143..>1683
                     /locus_tag="PKV23_10150"
     CDS             1143..>1683
                     /locus_tag="PKV23_10150"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQX85839.1"
                     /translation="MPKSLPARCDVVIIGGGIVGNSIAYHLGKIGITNTVVLERKQLT
                     SGTTWHAAGLVGQLRASKNLTELAKYTTSLFEGLEKETGQATGFKQNGSISIALTDGR
                     MEELLRGASMAKCFGLAVDVVSTAEIKERVPHYNMDGVKGGVFLPKDGQVNPIDVTQA
                     LASGARSRGAMIFENTKVTR"
BASE COUNT          342 a          488 c          477 g          376 t
ORIGIN      
        1 catacccgtc ctcctctccg gcaaagcgcc gttcggcggt ttccggatcg tcgtagacgt
       61 aaccggaggt cagatagatg gcttcggagg tctcgccgtg aagcgagcgg tccaaaccgc
      121 cacgcacaag ttttgttgcg tcgccccagc ctttggggtt ctgtctcttg gtatactttg
      181 ccatcagctt tgaacccagg gcagaccggc cactttccag ccattcacgg tgccgcggtg
      241 gccattggcg tccttgtcgc cttcaaagcc ttgcgcgaca ttccagcaat tggtgtagcc
      301 cgcatcagtg agggcggagg cggccgatga agaccgggcg ccggatcggc agatgcagag
      361 aatttccgca tccttgggca gacccatctg ctcgatttcc tcgacgaact tcgcgttgac
      421 ctgcatggct ggaaacactt gccaggcgag gcgaacgagc cgttccacgg cgggaacgcc
      481 cacgaacgtc cattcgggct gggtgcgcac gtccaccagc acagcggatg agttgccctg
      541 caaacgctga tgagcttcgg cgggcgaaat gttgcctcga tgcataaaac gacctgttct
      601 ttatttcgaa caggatgata gtcgaaacaa agtgcccggg caaaggaaaa tggctgctca
      661 gcgttatcaa tcagtgttta tggctcggat atttgacgat cgccggaccc gatactggat
      721 ccgtcagacc gaggccaccg cccaggtctt ccagcatggc gcggaacccg tcgagaatgc
      781 caaccatctg cggccttgct tcgacaatgc tttcatagtc gtcccacttg cccacgaaac
      841 aataggcgcg ttcaccggtt ttgatcaggg cgccgtcctt catgccgtca aggtccggca
      901 ggctcttgtg gagttcaatg aacttctgtt cctggcccgg cttcactttg aaacgtacaa
      961 cgttgtatgc ggtcatagct tcctcctttg gcgaggtccc cagcatgggc actttacgcc
     1021 tattaatccc tcctgtgtag ctctggtgaa ttctggtgct ttagggtgaa gctgactggc
     1081 gctttcagtt ccgcagcggc atatgttaga tgaattcacc acccccttct ggagttcccc
     1141 tcatgccgaa gtccctgccc gcccgttgcg atgtcgtcat cattggtggg ggcattgtgg
     1201 gcaattccat tgcctatcac ctgggcaaga tcggcattac caatacggtt gtgcttgagc
     1261 gcaaacagct cacctccggt accacctggc atgcggcggg ccttgttggg caattgcggg
     1321 cctcgaagaa cctcaccgaa ctggcgaaat acacaaccag cctgtttgaa ggtcttgaaa
     1381 aggaaacggg ccaggccacg ggcttcaagc agaatggctc gatctcaatt gcgctcacgg
     1441 atggacgcat ggaagagctc ctgcgcggcg cctccatggc caaatgcttt ggccttgccg
     1501 tcgatgtggt gagcacggcg gaaatcaagg aacgcgtgcc ccattacaac atggacggcg
     1561 ttaagggtgg tgtttttctg ccgaaggatg gccaggtcaa ccccattgat gtgacgcagg
     1621 ccctggcgtc cggtgcgcgg tcacgcggcg caatgatttt cgaaaatacc aaagtaacgc
     1681 gca
//
LOCUS       DAORVN010000551         1678 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_83738_length_1678_cov_2.853974, whole genome shotgun sequence.
ACCESSION   DAORVN010000551 DAORVN010000000
VERSION     DAORVN010000551.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1678
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83738_length_1678_cov_2.853974"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(7..1419)
                     /locus_tag="PKV23_10155"
     CDS             complement(7..1419)
                     /locus_tag="PKV23_10155"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HQX85840.1"
                     /translation="MLWHKSTMAAILGIHAAEGSRTMDESQLPDLKRALLAALPEDGS
                     TIGNMALRRTLAQTFAEDVAEDLYERARDELHDAGQVEKGRGRGGSVRRAAVAAGDAL
                     VLEAQAIPEEAKRRKPVQSGMPLVGRAVGQAAKPKSRGLPEEKVLAYRHEQRRINNPE
                     VGLVTPDDDADERTTRYAWDPHIDPALQFDVGRAQVENLIDAALASGDEGTMRAALEQ
                     LKRQAAPYLNWSGKAERTSFEVDTVSLHVHERIDPATILTALKKRLKPGKVGETPPQY
                     QDDFFQAWFDRALPLHEAIEFYKHDRGWANRLIAGDSLLVMNSMLQKEAMAGQVQMIY
                     IDPPYGIRYGSNFQPFVNKRAVKDRKDEDLTQEPEMIKAFRDTWELGIHSYLTYLRDR
                     LLLAKELLHESGSVFVQISDENLHYVREMLDEILGIHNYCSTIQVQKTGSQEGGLLGA
                     TVDFLIWYAKDKARQTKSLK"
BASE COUNT          285 a          524 c          498 g          371 t
ORIGIN      
        1 ataggtttac ttcaaactct ttgtttgcct cgccttgtct tttgcgtacc aaatgagaaa
       61 gtcgacggtg gcccctagca agcctccttc ctggctgcca gtcttttgca cttgtattgt
      121 tgagcaatag ttgtggatac cgagaatttc atccagcatt tcccggacgt agtgcagatt
      181 ctcatctgaa atctgcacaa acacgctacc cgattcatgc agcaactcct tcgccaacag
      241 aagtcgatcc cgcaaatagg tcagatacga atggatgccc agctcccacg tatcccgaaa
      301 tgccttgatc atttcgggtt cctgggtcag atcctcatcc ttgcggtcct tgacggcgcg
      361 cttgttgacg aacggctgga aattgctgcc gtagcggatg ccgtagggcg gatcgatgta
      421 gatcatctgc acctggccgg ccatcgcctc tttctgcagc atgctgttca tcaccagcag
      481 tgaatcgccg gcgatcaggc ggttggccca gccgcggtcg tgcttgtaga actcgatcgc
      541 ctcgtgcagc ggcagcgcgc ggtcgaacca ggcctggaag aaatcgtcct ggtactgcgg
      601 tggggtttca ccgaccttgc cgggcttgag gcgtttcttg agcgcagtca ggatcgtcgc
      661 cgggtcgatg cgctcatgca catgcaggga aacggtatcg acctcgaaac tcgtgcgctc
      721 ggccttgccg ctccagttca gatacggcgc ggcctgacgc ttcagttgct cgagcgccgc
      781 gcgcatggtg ccttcatcgc ccgaggccag cgccgcatcg atcaggttct cgacctgcgc
      841 gcgaccgaca tcgaactgca gggccggatc gatatgcgga tcccaggcgt agcgcgtcgt
      901 acgctcgtcc gcatcgtcgt ccggcgtgac caggccgacc tcggggttgt tgatgcggcg
      961 ctgctcgtgg cggtaggcca gcaccttttc ctcgggcagg cccctggatt tcggcttcgc
     1021 ggcctgaccg accgcacgcc cgaccaaagg catgccgctc tgcacgggtt tgcggcgttt
     1081 ggcttcctcg ggaatggctt gcgcttcgag caccagggca tcgcccgccg caaccgctgc
     1141 acggcgcacc gagccgcccc gaccgcgtcc cttttccact tggccagcgt cgtggagttc
     1201 atcgcgggcg cgttcgtaga gatcttcggc cacgtcctcg gcgaatgtct gcgcaagcgt
     1261 acgccgcaac gccatattgc cgatggttga accatcctcc ggcagggctg caagcaaggc
     1321 acgcttgaga tcgggaagct ggctttcatc catggtgcgt gaaccctctg cagcgtgaat
     1381 gccgagaata gccgccatcg tggatttgtg ccaaagcatt gctggcgcgt gcctgcgctc
     1441 gtataggaca ttgctgggga gcaaacccgt gtttggcgcg gaagttcact ggatatccgc
     1501 ttgcgcgggg gtgtcgaccg cagcgatacg gtttccgttg caggatcctg acctggtcgc
     1561 cccagcggaa atgtggcctg gcaagcttcg cctcagcgga gaggcgcgcg gccttggcgc
     1621 tgttacaatt ccgctcttgt ccacgctgca ttccccggta tccgtcatgt ccgtcccc
//
LOCUS       DAORVN010000552         1674 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_84080_length_1674_cov_4.201359, whole genome shotgun sequence.
ACCESSION   DAORVN010000552 DAORVN010000000
VERSION     DAORVN010000552.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1674)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1674)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1674
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84080_length_1674_cov_4.201359"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..496)
                     /locus_tag="PKV23_10160"
     CDS             complement(<1..496)
                     /locus_tag="PKV23_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019965895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HQX85841.1"
                     /translation="MLDRYALKLAKPVIDRAARVIRNSGYSADQVTFTGFGLGLFAVL
                     CIAFGFYAFAILPLLASRALDGLDGAVARAGVATDRGAFLDIGLDFVFYGLIPLAFAV
                     ANPEANALAAATLLAAFVGTGSSFLAYAIIAEKRGLKSTDFPNKSFYYLGGLTEGFET
                     IAMFL"
     gene            complement(489..1136)
                     /locus_tag="PKV23_10165"
     CDS             complement(489..1136)
                     /locus_tag="PKV23_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007066294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQX85842.1"
                     /translation="MTLLLENISLTIGDVLLVKSFTISIEAGEIVTLMGPSGCGKSSL
                     LAYIAGDLSPPMYGNGHVTLDGVDLVYLPAEQRHVGRLFQDDLLFPHLTVGENLLFGV
                     PRGSLSKRQHLVSEALASAQLEGFEDRDPGTLSSGQRARVALFRALMAKPKAMLLDEP
                     FSKLDAELRHSVRNYVFAHLRERQIPTLLVTHDREDAPAGGRVFRISNKGDMILA"
     gene            complement(1133..>1674)
                     /locus_tag="PKV23_10170"
     CDS             complement(1133..>1674)
                     /locus_tag="PKV23_10170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85843.1"
                     /translation="LALAVFWLETVPEKLDAVLLACGIAALALPSLLIADGQYLAFLR
                     LGLNGTWLGVFLAHLTPVFAYVFIVLKGPYRAFDPRYRAVALGLNADNYRFWSVVKLA
                     MMKPALAAAAAVGIGVSIAQYVPTQLIASGRLTTLPIEAVTLASGGSRPLMAVFALML
                     AVIPAFAFIIASRLGRKPL"
BASE COUNT          359 a          497 c          559 g          259 t
ORIGIN      
        1 cgaggaacat ggcgatggtt tcaaaccctt ccgtcaggcc accaagatag tagaaagact
       61 tgttgggaaa gtccgtgctt ttcaggccgc gtttctcggc gatgatggca taggcgagaa
      121 agcttgaacc ggtgccgacg aaggcggcaa gcagcgttgc ggcggcaagt gcgttggcct
      181 ccgggttagc gacggcaaag gccagcggaa tgagcccgta gaaaacaaag tccaggccga
      241 tgtcgaggaa ggccccgcgg tcggtggcaa cgccggcccg cgccacggcg ccatcaagcc
      301 cgtcaagggc ccggctggcg agcaggggaa ggatggcaaa ggcataaaac ccaaaggcga
      361 tgcatagcac cgcgaataac ccaagcccga atcccgtgaa ggtcacctgg tcggcggagt
      421 aaccgctgtt ccggatcacc cgtgccgccc tgtcgatgac cggcttggcg agtttcagcg
      481 catagcggtc aagcaaggat catgtccccc ttgttgctga tgcggaaaac ccgcccgccc
      541 gccggtgcgt cttcgcggtc atgggtgacc agcaatgtgg gaatttggcg ttcgcgcaaa
      601 tgcgcaaaga catagttccg caccgaatgc cggagctcag cgtcaagctt ggagaacggc
      661 tcatccagca gcatggcctt gggctttgcc atcagggcgc ggaacagcgc cacccgggct
      721 ctctggccgc tggaaagcgt gccggggtcg cggtcctcaa agccttcgag ctgggcgctg
      781 gccagcgcct cgctcaccag gtgctggcgc ttgctcaggc tgccgcgggg aacgccaaac
      841 aagagatttt cgccaaccgt gagatgcggg aacagcaggt catcctggaa caggcggccc
      901 acgtgccgct gttccgccgg caggtaaacc aggtccacgc cgtcaagcgt cacgtgcccg
      961 ttgccataca tgggcggcga caggtcgccg gcgatatagg cgaggagcga tgattttccg
     1021 cagccgctgg gccccatcag cgtgacgatc tcaccggctt cgatggaaat cgtgaagctc
     1081 ttgacgagca gcacgtcgcc aatggtgagg ctgatgtttt caagcagcag ggtcatagcg
     1141 gtttccggcc aaggcgcgag gcgatgataa aggcaaaggc cggtatgacg gcaagcatga
     1201 gcgcaaagac cgccatcagc ggccggctgc cgccgcttgc cagtgtcacc gcttcgatgg
     1261 gaagcgtagt cagccggccc gaggcaatga gctgggttgg aacatattgc gcaatgctga
     1321 caccgatgcc gaccgcggcg gctgccgcca gcgccggctt catcatggcg agcttcacca
     1381 ctgaccagaa gcggtaattg tcggcattga ggccaagcgc cacggcgcgg tagcgagggt
     1441 caaaggcccg gtagggtccc ttcagcacga tgaagacata ggcgaagacc ggcgtgaggt
     1501 gggcgaggaa caccccgagc caggtgccgt tgagcccgag acgcaggaac gcgagatact
     1561 gcccgtcggc aatgaggagc gacggcagcg ccagggccgc aatgccgcag gccagcagga
     1621 cggcatcaag tttttccggc acagtctcca gccagaagac cgcgagcgct aggg
//
LOCUS       DAORVN010000553         1672 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_84282_length_1672_cov_2.778602, whole genome shotgun sequence.
ACCESSION   DAORVN010000553 DAORVN010000000
VERSION     DAORVN010000553.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84282_length_1672_cov_2.778602"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="PKV23_10175"
     CDS             complement(<1..222)
                     /locus_tag="PKV23_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003540322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HQX85844.1"
                     /translation="MSRPRIAILGIFVADLAFRAKRLPVMGETLLGQGFAMGPGGKGS
                     NQSVAAARAGGDVSFIAKLGRDSFGDIALK"
     gene            complement(219..671)
                     /locus_tag="PKV23_10180"
     CDS             complement(219..671)
                     /locus_tag="PKV23_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015318188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RbsD/FucU family protein"
                     /protein_id="HQX85845.1"
                     /translation="MLKSIDPLLNADVLFALRSMGHGDELIICDTNFPADSVAKETVL
                     GQLLRIDASAARVARAVLSVMPLDSFVDTPAERMEIVGKPKEVPPVQAEVQAEIDKAE
                     GKSWPMGSIERFAFYEKAKKAYCVIQTGERRFYGCFVFKKGVIPPDAP"
     gene            complement(713..1177)
                     /locus_tag="PKV23_10185"
     CDS             complement(713..1177)
                     /locus_tag="PKV23_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015667837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HQX85846.1"
                     /translation="MKIEALTKAGFAPFGEVIEIDGAQHFPINQGFAERFNDLASVDA
                     ETVNISIVVARPRPRPIAITLMERHPLGSQIFYPLQDRPWLVVVCGNPKDRASFRAFS
                     ATGRQGINYARNTWHHPLLVLEAESRFLVVDRKGPGTNLEEAAIDEIRLDLL"
     gene            complement(1174..>1672)
                     /locus_tag="PKV23_10190"
     CDS             complement(1174..>1672)
                     /locus_tag="PKV23_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013528244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HQX85847.1"
                     /translation="IVEIARLQSKDMQQISHIRGLDRQDLSRAMPRLREAIERGLDVP
                     EAFVVNEKDIAPIPMPDTNGVWATTRFVSPDDLRHDMHVTIVTFQPGGVIPFAETHVM
                     EHGLYVLEGKAVYRLNQDWVEVEAGDYMWLRAFCPQACYAGGPGPFRYLLYKDVNRHM
                     KLGGR"
BASE COUNT          322 a          543 c          481 g          326 t
ORIGIN      
        1 cttgagcgcg atatcgccga aggaatcgcg gcccagcttg gcgatgaagc tcacgtcacc
       61 gcccgccctt gcagccgcga ctgactggtt cgagcccttg ccccccggcc ccatggcaaa
      121 gccctggccc agcagcgttt cgcccatcac cggcaggcgc ttggcccgga aagcgaggtc
      181 ggccacgaag attccgagaa tggcaatgcg cgggcggctc atggcgcgtc cggaggaatg
      241 acgcctttct tgaacacgaa gcagccatag aagcgccgct cgcctgtctg gatcacgcaa
      301 taggccttct tggctttctc gtagaaggca aagcgctcga tggagcccat gggccaggat
      361 tttccctcgg ccttgtcgat ctccgcctgc acttccgcct gcaccggcgg aacttccttg
      421 ggcttgccga cgatttccat gcgctccgcc ggcgtgtcga caaaactgtc gagcggcatc
      481 accgacagca ccgccctggc gacgcgcgcc gcgctcgcgt caatgcgcag caactggccc
      541 agcaccgttt ccttggccac cgaatccgcc ggaaaattcg tatcacaaat gataagctca
      601 tcgccatgcc ccatggagcg caatgcaaag agaacatcgg cgttgagaag cgggtcaatt
      661 gatttcagca agatattctc ccatttttat tttttgtaat tgaaccaaaa atctacagta
      721 ggtccagcct tatttcatca atcgcggctt cttccaggtt tgtccccggc cctttccggt
      781 ccacaaccag gaagcggctt tccgcctcca gcaccagaag cggatgatgc caggtgttgc
      841 gcgcgtaatt gatgccctgc cggcccgtgg ccgaaaaagc acggaagctg gcgcggtcct
      901 ttgggttccc gcacaccacg acaagccacg gcctgtcctg cagcggatag aaaatctggc
      961 ttcccagggg gtgccgctcc atcagcgtga tcgcgatggg ccgggggcgc ggcctggcga
     1021 ccacaatgct gatgttgaca gtctcggcat cgaccgaggc cagatcattg aagcgctcgg
     1081 caaagccctg gttgatcgga aaatgctgcg ccccgtcaat ttcgatgacc tcgccgaagg
     1141 gcgcgaagcc cgcctttgtc agcgcctcga ttttcatctg ccgccgagct tcatgtggcg
     1201 gttcacgtcc ttgtagagca ggtagcggaa tggccccggc ccacccgcat agcaggcctg
     1261 cgggcagaag gcgcgaagcc acatgtaatc gcccgcctcc acctccaccc aatcctggtt
     1321 gagccggtac accgccttgc cctccagcac atagagcccg tgttccatca catgggtttc
     1381 ggcgaagggg atgacgccgc ccggctggaa ggtgacgatg gtcacatgca tgtcgtggcg
     1441 caggtcgtcc gggcttacaa agcgcgtcgt cgcccacacg ccgttggtat ccggcatcgg
     1501 gattggcgca atatcctttt cattcaccac gaaggcttcc ggcacatcga gcccccgctc
     1561 gatggcctcg cgcaggcgcg gcatggcgcg cgacaggtcc tgccgatcga ggccgcggat
     1621 atgcgaaatc tgctgcatgt ctttggactg tagtcgggcg atttcgacaa tc
//
LOCUS       DAORVN010000554         1668 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_84609_length_1668_cov_2.971482, whole genome shotgun sequence.
ACCESSION   DAORVN010000554 DAORVN010000000
VERSION     DAORVN010000554.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1668
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84609_length_1668_cov_2.971482"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            4..990
                     /gene="obgE"
                     /locus_tag="PKV23_10195"
     CDS             4..990
                     /gene="obgE"
                     /locus_tag="PKV23_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012114079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HQX85848.1"
                     /translation="MSFRRENFIEMGGPDGGDGGNGGDVWVEAVDALNTLIDYRYQQH
                     FKVRTAGHGMGRLRAGANSDDIVLKVPVGTEVYEADNETLIADLAKLGDRVRIARGGN
                     GGFGNHHFKSATNRAPRHANPGIEGEERWIWLRLKLIADAGLLGLPNAGKSTLLAAVS
                     AAKPKIADYPFTTLHPQLGVVKAGGNSFVLADIPGLIEGANEGAGLGTRFLGHVERCA
                     VLLHLIDVTGDDPVKAYRIIRKELKDYGPGIEGKPEIIAFNKIDAISDKDLDKKLADF
                     KKKLKRTPILISGATGKNVDDTMKRLLTVIQADRKAARKQEDQGVALEDWHP"
     gene            978..>1668
                     /gene="proB"
                     /locus_tag="PKV23_10200"
     CDS             978..>1668
                     /gene="proB"
                     /locus_tag="PKV23_10200"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003505252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HQX85849.1"
                     /translation="MASVKQRLQAAKRIVVKIGSALLVEANTGEVKSAWLSSLIDDLA
                     TARSGGADIIVVSSGAIALGRRTLGLPKGRLRLEESQAAAAVGQIALAQAWSEAFRTK
                     KIVAAQVLVTLTDTEERRRHLNARATLTTLLAQGAVPVINENDTVATSEIRYGDNDRL
                     AARVASMMSADCLVLLSDIDGLYSAPPDRAGATFVPEVREITPEIEAMAGKPVSVVGS
                     GGMVTKIEGGKI"
BASE COUNT          350 a          524 c          490 g          304 t
ORIGIN      
        1 gctgtgtctt ttcggcgcga aaatttcatc gaaatgggcg gtcccgatgg cggagacggt
       61 ggcaatggcg gagacgtttg ggtggaagcc gtcgatgccc tcaacacgct catcgactac
      121 cgctaccagc agcacttcaa ggtccgcact gccggccacg gcatgggacg tctgcgcgcc
      181 ggagcaaatt cggacgatat cgtgctcaag gttcccgtcg gcaccgaggt ctatgaagcc
      241 gacaatgaaa ccctgattgc cgatctcgcc aaactcggcg accgcgtgcg cattgcccgg
      301 ggcggaaacg gcggcttcgg caaccaccat ttcaaatccg ccaccaaccg cgcgccccgc
      361 cacgccaacc cgggcatcga gggtgaggaa agatggatct ggttgcgcct gaagctcatt
      421 gccgatgccg ggctcctcgg cctgcccaat gccggaaaat caacactgct ggccgccgtt
      481 tccgccgcca agccgaaaat tgctgactat cccttcacca ccctgcatcc ccagcttggc
      541 gtggtgaaag ccggcggcaa cagctttgtc ctcgctgata ttccgggcct cattgagggc
      601 gccaacgaag gcgctggcct cggcacgcgc ttcctcggcc atgtcgaacg ctgcgccgtt
      661 ctgctgcatc tcatcgacgt gaccggcgac gacccggtga aggcctaccg catcatccgc
      721 aaggaactga aagattacgg tcccgggatc gaaggaaagc ctgagatcat cgccttcaac
      781 aagattgacg ccatcagcga caaggacctg gataagaaac ttgcagactt caagaaaaag
      841 ctgaagagga cccccatcct gatttccggc gccaccggaa agaatgtcga tgataccatg
      901 aagcgcctgc tcaccgtcat tcaggccgac cgcaaggcgg caaggaagca ggaggaccag
      961 ggcgttgcac tggaggattg gcatccgtga agcagcgcct gcaagcggcc aagcgcatcg
     1021 tcgtcaagat cggctcggcg cttttggttg aagccaacac tggcgaagtg aaatccgcct
     1081 ggctttcaag cctaatcgat gaccttgcaa cggccagatc cggcggtgcc gacatcatcg
     1141 tcgtctcctc cggcgccatt gccctgggcc ggcgcaccct tggccttccc aaaggcaggt
     1201 tgcggcttga agaaagccag gccgccgcag ccgtgggcca gattgccctc gctcaagcct
     1261 ggtcggaggc tttccgcaca aaaaaaatcg tggcggccca ggtgctggtg actttgaccg
     1321 acaccgaaga gcgccgccgc catcttaatg cccgcgctac cttgaccacc cttctcgccc
     1381 agggtgctgt tccggtgatc aatgaaaatg atacggtggc cacctctgaa atccgctacg
     1441 gcgacaatga ccggctggcg gcgcgcgttg cctccatgat gtcggccgat tgcctggtgc
     1501 tgctttcgga tattgatggg ctttattcgg ctccgcccga ccgggccggc gccactttcg
     1561 ttccggaagt cagggaaatc acccccgaga tcgaggccat ggccggaaaa cccgtttccg
     1621 ttgtcggttc ggggggcatg gtgaccaaga tcgaaggagg gaaaattg
//
LOCUS       DAORVN010000555         1664 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_84978_length_1664_cov_5.957116, whole genome shotgun sequence.
ACCESSION   DAORVN010000555 DAORVN010000000
VERSION     DAORVN010000555.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84978_length_1664_cov_5.957116"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1122)
                     /gene="bktB"
                     /locus_tag="PKV23_10205"
     CDS             complement(<1..1122)
                     /gene="bktB"
                     /locus_tag="PKV23_10205"
                     /inference="COORDINATES: protein motif:HMM:NF006552.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketothiolase BktB"
                     /protein_id="HQX85850.1"
                     /translation="MSNRDIVVLSAVRSPIGAFGGSLADFESTELAGIVMKEAIARSG
                     VDPLAINYVTVGTTMPTDSRYAYVSRVASIQAGLSMDSVAMQVSRLCASGLQGIVTTA
                     QNLMLGDADYGIGGGVEVMSKATYLMPALRSGARMGDTKAIDSMVAVLTDPFGVGHMG
                     ITAENLATKHGFSREDQDALAVESQRRATVAIAEGRFKSQIFPIVKQTRKGEVVFDTD
                     EHVKSNTTMESLAKMKPAFKKDGTVTAGNASGINDGAAFFVLAAADVAAKAGQKAMAR
                     IASYAVAGVPNDIMGEGPIPATKLALKKAGLSLDQMDVIEANEAFAAQAIAVNKGLGL
                     DPVRTNPNGGAIALGHPVGCSGAFIAIKAIYELQRTCGKY"
     gene            complement(1198..>1664)
                     /locus_tag="PKV23_10210"
     CDS             complement(1198..>1664)
                     /locus_tag="PKV23_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012322498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HQX85851.1"
                     /translation="MLAHKAVHEAHVAAEVAAGHKAAFDASVIPSVAYTHPEVAWVGY
                     TEDQAKKEGRKVESAKFPWAASGRAIANGCDYGFTKLIFDAETHRVIGGAIVGPNAGD
                     MIGEVCLAIEMGCDSVDIGKTIHPHPTLGETVGMAAEVAHGTCTDVPPPRKR"
BASE COUNT          309 a          495 c          531 g          329 t
ORIGIN      
        1 gtacttgccg caggtccgct gcagctcgta gatcgccttg atggcgatga aggcaccgga
       61 gcaaccgacc gggtgaccca aagcgatggc accgccgttc gggttggttc tcaccggatc
      121 gagaccgagg cccttgttga ccgcgatggc ctgcgcagca aaggcttcgt tggcttcgat
      181 gacatccatc tggtccagcg acaggccagc cttcttcagg gccagcttgg tcgccgggat
      241 cgggccttcg cccatgatgt cattcggcac gccggcgacg gcataggaag cgatgcgagc
      301 catcgccttt tgaccggcct tggcagcgac atcggccgcg gccagcacga agaaggccgc
      361 gccgtcgttg atgccggaag cgttaccggc ggtgacagtg ccgtccttct tgaaggccgg
      421 cttcatcttg gccagtgatt ccatcgtcgt gttggacttg acgtgctcgt cggtgtcgaa
      481 caccacttcg cccttgcggg tctgtttgac gatcgggaag atctgcgact tgaaacgacc
      541 ttcggcgatg gcaaccgtag cgcgacgctg ggattcaacc gccagcgcat cctgatcttc
      601 acggctgaag ccatgcttgg tggcgaggtt ttcagcggtg atacccatgt ggccaacgcc
      661 gaaggggtcg gtcagcacgg caaccatgga gtcgatggcc ttggtgtcac ccatgcgcgc
      721 gccggaacgc agtgccggca tcaggtaggt ggccttggac atgacttcaa caccgccgcc
      781 gataccgtaa tcggcatcac ccagcatcag gttttgggcg gtggtgacga taccttgcag
      841 gccggaggcg cacagacggg aaacctgcat ggcgacggaa tccatcgaca ggccggcctg
      901 gatggaagca acgcgggaaa cgtaggcgta gcgggaatcg gtcggcatcg tggtgccgac
      961 cgtaacgtag ttgatggcca gcggatcaac accggagcgg gcaatcgctt ccttcatgac
     1021 aatgccggcg agttccgtgc tttcaaaatc agccagggaa ccaccaaaag cgccgatggg
     1081 ggaacgaact gcgcttagaa cgacaatatc tcgattagac atatggatta cctctagttg
     1141 gagctctcac tggagagatt tgcgccagtc catgactaga ctggcgcgct tttgtggtta
     1201 ccgcttgcgc ggcgggggta cgtcggtgca ggtgccgtgc gccacttcgg ccgccatgcc
     1261 gaccgtttcg ccgagcgtcg ggtgcgggtg gatggtcttg ccgatatcga ccgagtcgca
     1321 gcccatttcg atggccaggc agacttcgcc gatcatgtcg ccggcgttcg ggccgacgat
     1381 ggctccgccg atgacgcgat gcgtttcggc atcgaagatc agcttggtga aaccgtaatc
     1441 gcaaccgttg gcaatcgccc gaccggaagc agcccacggg aacttggctg actcgacctt
     1501 gcggccttct ttcttggcct gatcttccgt gtaaccgacc catgccactt ccggatgggt
     1561 gtaggcgacg cttggaatca ccgacgcgtc gaacgcagcc ttgtggcccg ccgcaacttc
     1621 cgccgcgacg tgggcttcat gcaccgcctt gtgggcgagc atgg
//
LOCUS       DAORVN010000556         1663 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_85095_length_1663_cov_2.951493, whole genome shotgun sequence.
ACCESSION   DAORVN010000556 DAORVN010000000
VERSION     DAORVN010000556.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1663)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1663)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1663
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85095_length_1663_cov_2.951493"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(48..1163)
                     /locus_tag="PKV23_10215"
     CDS             complement(48..1163)
                     /locus_tag="PKV23_10215"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008189685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047304 - 2-aminoethylphosphonate-pyruvate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HQX85852.1"
                     /translation="MPYLLTPGPLTTSRTVKLAMLADWGSRDVEFRKIVSNIRKRILQ
                     LANCDGRYECVIMQGSGTFAIEAALGSLCPDKRRKTLVIANGAYGDRAAQILERIGRP
                     FLKIDKGDSTVPTVEEVVTMLDADKSISHVWMIHCETTSGIVNPIEEIGQAVKSHDKV
                     FMVDAMSSFGALPLHMVEQNIDVMVSSSNKCLEGVPGFSYVVVKRDLLEASAGACHSV
                     VLDLFDQWRGLENNGQFRFTPPTHALVAFDQALKEHEQQGGVEGRGSRYGRNAKALVA
                     GMTEMGFSPLLQEGLAGPIIQTFLTPRDPNFHFEQFYEALRVRGFTIYPGKLTKRPSF
                     RIGTIGQIDERVIAAALQAIRDVVSEMGVKDFTPLES"
     gene            1313..>1663
                     /locus_tag="PKV23_10220"
     CDS             1313..>1663
                     /locus_tag="PKV23_10220"
                     /inference="COORDINATES: protein motif:HMM:NF012354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HQX85853.1"
                     /translation="MTYAQLKAFHAVALEGGFSKAAIGLSLTQPAISDHVRKLEEAHG
                     VQLFLRAARGVTLTETGRKLYAITERLFEAEAQASELLMRAETLEEGHLTVGADAAVH
                     VLQHINRFKKEFPRI"
BASE COUNT          337 a          480 c          488 g          358 t
ORIGIN      
        1 actgaaacgg ttttgctggc tgacttcatc tttgtgtcgg gcatcattta actttcaagc
       61 ggagtgaaat ccttcacccc catttcactg acgacatcgc ggatcgcctg aagcgcggcg
      121 gcaatgacgc gctcgtcaat ttgcccgatg gtcccgatgc ggaaactcgg gcgcttggtc
      181 agctttcccg gatagattgt aaagccgcgg acccgcagcg cctcgtaaaa ctgttcgaaa
      241 tgaaagttgg ggtcgcgcgg ggtgagaaag gtctggatga tcggcccggc caaaccttcc
      301 tgcagcaatg gtgaaaatcc catttccgtc atgccggcga caagggcctt ggcgttgcgg
      361 ccataacgcg atccgcgccc ttccacgccg ccttgctgct catgctcctt aagggcctgg
      421 tcaaaggcga cgagcgcatg ggtgggcggc gtgaagcgga actgcccatt attctcaagg
      481 ccgcgccact ggtcgaacag gtcaagcacg actgagtggc aggcacccgc cgaagcttcc
      541 agcaggtcgc gtttcaccac cacgtaggaa aagcctggaa ctccttcgag gcatttgttc
      601 gaggacgaaa ccatgacgtc aatgttctgc tccaccatgt ggaggggcag ggccccgaac
      661 gaggacatgg catccaccat gaagaccttg tcatgcgact tgaccgcctg gccgatctct
      721 tcgatggggt tcacgatacc cgaggtggtt tcgcaatgga tcatccagac atggctgatg
      781 ctcttgtccg catcgagcat tgtgacgact tcttcaaccg tgggaaccgt gctgtcgccc
      841 ttgtcgatct tcaggaatgg cctgccgatg cgttccagga tttgcgctgc ccggtcgcca
      901 taggcgccat tggcgatcac cagggtcttg cgccgcttgt ccgggcacag gctgcccagc
      961 gccgcctcaa tggcgaaggt cccggaaccc tgcatgatca cgcattcata gcgcccgtcg
     1021 caattggcca gttgcaggat gcgcttgcgg atattgctga cgattttccg gaattccaca
     1081 tcgcgcgagc cccagtcggc cagcatggcg agctttaccg tccgcgaggt tgtcaacggg
     1141 ccgggggtga gcaggtaggg catgtcttcc tggccctcgg ggccggggaa ggatggcttg
     1201 aaagacggcg tcttggtgtt catcgggggg ctccagatta tgcggctaca ttaagcattg
     1261 accattatca gtcaaatcgt atgttcttat gcaaaccata ggaaattctg atatgacata
     1321 tgcccagctc aaagcgttcc atgcggtggc ccttgaaggc ggtttctcta aggccgccat
     1381 cgggctctcc ctgacccaac cggcaatttc agatcacgtg cggaaacttg aggaagcgca
     1441 cggcgttcaa ttgttcctgc gcgccgcgag aggcgtcacg ctcactgaga ctggccgcaa
     1501 actctatgcc atcaccgaac gcctgtttga ggcggaggcc caggcatcgg aactcctcat
     1561 gcgggcggag accctcgaag aaggacatct gacggttggc gctgatgctg ccgttcatgt
     1621 gttgcagcat attaacaggt tcaagaagga gttcccgcgc atc
//
LOCUS       DAORVN010000557         1660 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_85353_length_1660_cov_2.679751, whole genome shotgun sequence.
ACCESSION   DAORVN010000557 DAORVN010000000
VERSION     DAORVN010000557.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1660
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85353_length_1660_cov_2.679751"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..590
                     /locus_tag="PKV23_10225"
     CDS             <1..590
                     /locus_tag="PKV23_10225"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HQX85854.1"
                     /translation="FMVIAGNAYLGRTVKALYGRVMPVASYIIATEPLGENLAKSLIA
                     NNEAVANTNFIVDYFRRSNDHRMLFGGRASYSTFEPPNLGEYMRPRMTHVFPQLKDVK
                     IDHAWGGYIAITSNRIPDCGRLSPTVYYAHGYSGQGVALTGLYGKLMAEAIRGTAERF
                     DLLAKVKHLPFPGGPMRTPMLVAAMMYYRIRDALS"
     gene            complement(597..>1660)
                     /locus_tag="PKV23_10230"
     CDS             complement(597..>1660)
                     /locus_tag="PKV23_10230"
                     /inference="COORDINATES: protein motif:HMM:NF013354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyramine oxidase"
                     /protein_id="HQX85855.1"
                     /translation="YGDPRGGAYRRNAFDTGEYGVGQFLDSLTLGCDCLGHIHYFDAW
                     HHDWHGKPKHIRNAICMHEEDFGILWKYSDPARGTVTVKRSRRLVISSISTIGNYVYG
                     FFWYFYQDGTIGVEVKATGIPLGSGMAQDEPAQYGVKVAPGVEAHVHQHVFSFRFDMA
                     VDGERNAVSEVNFAAVPMGPGNPHGNAIKVTENQLQGESDAQRNMDLASARYWRVTNP
                     NKLNSLGDPVGYKLVPGVNALPFNHPDSAIGRRAAFMYKHFWATQYARDELYPAGWYP
                     NLHGGGDGLPLWTAQNRSLVNENIVVWYTMNYHHLPRPEDWPVQPCIYASFHWMPSGF
                     FDSNPALDVPSSPPASTCC"
BASE COUNT          354 a          469 c          507 g          330 t
ORIGIN      
        1 agttcatggt catagccggc aacgcttacc tcgggcgtac ggtgaaagcc ctttatggcc
       61 gggtgatgcc ggttgcaagt tacatcatcg caacggagcc gcttggcgaa aacctggcaa
      121 agtccctcat tgccaacaat gaggccgtgg ccaacaccaa tttcatcgtg gattatttcc
      181 ggcggtccaa tgaccaccgc atgctgtttg gcggacgggc gagctattcg actttcgagc
      241 cgcccaatct tggggaatac atgcggccgc gcatgaccca tgttttcccg caattgaaag
      301 atgtgaagat cgaccatgcc tggggcggct atatcgccat tacctcaaac cgcattccgg
      361 attgcgggcg gctttcgccc actgtttact atgcgcacgg ctattcgggc cagggcgtgg
      421 cgctgacggg cctctacggc aagctcatgg cggaagccat tcgcggaacc gcggaacgat
      481 ttgaccttct ggccaaggtc aagcacctgc ccttccctgg cgggcccatg cggacgccga
      541 tgctggtggc ggcgatgatg tattaccgga tcagggacgc actgagctga cgccggtcag
      601 caacaggttg aagccggcgg cgaagacggc acgtcaaggg cgggatttga atcaaaaaag
      661 cccgacggca tccagtggaa cgaagcgtag atacagggct ggaccggcca atcctccggg
      721 cgcggaagat ggtgatagtt catcgtgtac cagaccacga tgttttcatt gacgagcgaa
      781 cggttctgcg cggtccagag cggcaggccg tcgccgccac catgaagatt tggataccag
      841 cccgcaggat aaagctcatc cctggcatat tgcgtggccc agaaatgctt gtacatgaat
      901 gccgcgcggc gtccgatggc tgagtccgga tggttgaaag gcagcgcatt cacgcccggg
      961 accagcttgt agccaacagg gtcgccaagc gaattgagtt tgttggggtt ggtcacccgc
     1021 caatagcggg cgctggcgag atccatgttg cgctgagcat cgctttcacc ctgcagctgg
     1081 ttttccgtga ccttgatggc attgccgtgg ggattgcccg ggcccatcgg cacggcggcg
     1141 aaattcactt ccgagacggc gttgcgctca ccatccacgg ccatgtcgaa gcggaaggaa
     1201 aagacatgct gatggacatg ggcctctact ccgggcgcca ccttgacgcc atactgggcc
     1261 ggttcatctt gcgccattcc gctgccgagg ggaatgccgg ttgccttcac ttcaacgccg
     1321 atggtgccgt cctgatagaa ataccagaag aagccataaa cataattgcc gatggtcgag
     1381 atgctggaga tgacaagacg ccgggagcgc ttgaccgtga cggttccgcg cgccggatcg
     1441 gagtacttcc agagtatgcc aaagtcttcc tcatgcatgc agatggcatt gcggatgtgt
     1501 ttgggtttcc catgccaatc atgatgccag gcatcgaaat aatgaatatg cccgaggcag
     1561 tcgcagccga gcgtcaggga atcaagaaac tggcccacgc catactcgcc cgtatcgaaa
     1621 gcattgcggc ggtaggcgcc gccgcgggga tcgccgtagg
//
LOCUS       DAORVN010000558         1660 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_85372_length_1660_cov_2.369470, whole genome shotgun sequence.
ACCESSION   DAORVN010000558 DAORVN010000000
VERSION     DAORVN010000558.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1660
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85372_length_1660_cov_2.369470"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..404)
                     /locus_tag="PKV23_10235"
     CDS             complement(<1..404)
                     /locus_tag="PKV23_10235"
                     /inference="COORDINATES: protein motif:HMM:NF020067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HQX85856.1"
                     /translation="MTRVQGRRQRVEFLDIGQSRKVRIAARASAVLFLGMTILHSVIL
                     GGYLNYDGSPWAKVPGKLSSFIGWAADDIRITGLVHQEPEMVLMALGLKPGGSLIGFD
                     AENAKALLENLDWVATATVQRSFPNQLEISVE"
     gene            complement(401..1342)
                     /locus_tag="PKV23_10240"
     CDS             complement(401..1342)
                     /locus_tag="PKV23_10240"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012111342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HQX85857.1"
                     /translation="MIKRIPKDTHVAVLMGGWSAEREVSLSSGEGCAAALRRAGFNVT
                     LIDAQRETIFSVLQDLRPDVAFNALHGKWGEDGCASALLETLQIPYTHSGVLASSLAM
                     NKEKSKHLFHEAGIPVAESLLVEIEVAAAQHPMALPYVLKPVDEGSSVGVHIVTEQSN
                     GPAAIILEQRNLFGSHVMAERFIPGRELTCAVMGDVALGVIDIVSKVGFYDYRAKYSP
                     GGSQHILPADIPKDVYRKCQQYSLQAHRTLGCRGVSRADFRYDDTHGPAGELILLEIN
                     TQPGMTGTSLVPELAAHSGHSYEELVTWMVNDASINR"
     gene            complement(1339..>1660)
                     /locus_tag="PKV23_10245"
     CDS             complement(1339..>1660)
                     /locus_tag="PKV23_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011995982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylenolpyruvoylglucosamine reductase"
                     /protein_id="HQX85858.1"
                     /translation="ILAAMNKITDSREATQPIKSRTGGSTFKNPPGNKSWQLIDQAGL
                     RGFAIGPARVSELHCNFLINEGGATATQIEELGETIRKRVKESSGIELDWEIKRIGIP
                     AGAI"
BASE COUNT          344 a          501 c          464 g          351 t
ORIGIN      
        1 tcctccaccg aaatttcaag ctggttagga aatgaccgtt gcaccgtggc ggttgccacc
       61 caatcgaggt tttccagaag cgctttggcg ttttccgcat caaaaccaat caggctgcca
      121 ccgggcttca gtcccagggc catgagaacc atctccggct cctggtggac caatcccgtg
      181 atccggatgt catcggccgc ccagccgatg aaacttgaga gttttccagg cacttttgcc
      241 caggggctgc cgtcataatt cagatagccg cccagaatga cgctgtgcag aatggtcatg
      301 ccgagaaaca gcacggcact tgccctggcg gctatgcgaa ctttcctgct ctggccaatg
      361 tccagaaact cgacgcgctg cctgcgccct tggacccgcg tcatcggttg atcgatgcgt
      421 cgttgaccat ccatgtcacc agctcctcat atgaatgtcc ggaatgcgcc gccagctcgg
      481 gaaccagaga agttccagtc attcccggct gcgtattgat ttccaaaagg atcagctcgc
      541 ctgccggccc gtgcgtgtcg tcgtagcgga agtcggcgcg gctgacgccg cggcagccca
      601 acgtccggtg ggcttgaagc gaatactgct ggcacttccg gtaaacatcc ttgggaatat
      661 cggccggaag aatgtgctgg gagccaccgg gagagtactt ggcgcggtaa tcgtaaaaac
      721 cgaccttgga aacgatgtca atgacgccaa gggcgacgtc acccatcacg gcacaggtaa
      781 gctcccgccc cggaataaac cgttcggcca tcacgtgcga accgaaaaga ttgcgctgct
      841 caagaatgat cgcggccggg ccattggact gttccgtgac gatgtgaacg ccgacgctag
      901 agccttcatc gacaggtttc agcacataag gcagcgccat gggatgctgg gcggcagcca
      961 cttcgatttc aaccagcagg ctttctgcaa ccggaatgcc ggcttcatgg aacaagtgtt
     1021 tcgacttctc cttgttcatc gcaagtgacg aggccagaac cccggaatgc gtgtagggaa
     1081 tttgcagtgt ttccaaaagc gccgaggcac agccatcctc gccccatttt ccgtggagcg
     1141 cgttgaacgc cacatcaggg cgcaagtcct gcaggacgga aaaaatcgtc tcacgctgcg
     1201 cgtcgatcag cgtgacgttg aatcctgccc gccgcagcgc cgcggcacaa ccttctcccg
     1261 aggaaaggct cacctcgcgt tcagccgacc agccgcccat cagcacggcg acatgcgtgt
     1321 ccttgggaat gcgcttgatc atatggcacc tgccggaatg ccgatgcgct tgatctccca
     1381 atcaagctcg atgccggagc tttctttcac acgcttccgg atcgtttcac caagctcttc
     1441 aatttgcgta gctgttgccc cgccctcgtt gatcaggaag ttgcagtgaa gttcagagac
     1501 cctggcagga cctatggcaa agccgcgcag gcccgcttgg tcgatcagtt gccatgattt
     1561 gttgcccggc ggattcttga aggtggagcc gccggtacgg ctcttgatcg gctgggttgc
     1621 ttcgcggctg tcggtaatct tgttcatcgc tgccagaatt
//
LOCUS       DAORVN010000559         1659 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_85457_length_1659_cov_2.596010, whole genome shotgun sequence.
ACCESSION   DAORVN010000559 DAORVN010000000
VERSION     DAORVN010000559.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1659)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1659)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1659
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85457_length_1659_cov_2.596010"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..546
                     /locus_tag="PKV23_10250"
     CDS             <1..546
                     /locus_tag="PKV23_10250"
                     /inference="COORDINATES: protein motif:HMM:NF018420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1194 domain-containing protein"
                     /protein_id="HQX85859.1"
                     /translation="EWAGDGAHQVTVPWRLIDGPETARAFAEELAGKPAARFRWGTSI
                     SSVLRFSAGLFEGSGSRQVIDISGDGPNNVGGTVTVARDAVVAQGIVINGLPMLLKPA
                     QVLPPLDRYFVDCVIGGEGSFSLPVTSIDSLAEAIRMKLIRELLSSDANDAGRIIPAA
                     TEPPTDCLAGEKERARRQDQY"
     gene            complement(547..1164)
                     /locus_tag="PKV23_10255"
     CDS             complement(547..1164)
                     /locus_tag="PKV23_10255"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006466802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class GN sortase"
                     /protein_id="HQX85860.1"
                     /translation="MVPATLTLPKVGPVRLSRRSFLNFLSAVCLGLSVFLFGQSAWIF
                     AKAQLAQVLLERAFTQTIATGELVKAWRWADTWPVARIEISRIQASAIVLHGGSGEAL
                     AFGPALLAETSRIGEPGTAVISAHRDTHFAFLKDVVVGDRITITRDDGLVFIYRVTGT
                     SIVDWNNSGIDRHAMGFNLVLATCYPFDAITRGSERYLVHAELVK"
     gene            complement(1166..>1659)
                     /locus_tag="PKV23_10260"
     CDS             complement(1166..>1659)
                     /locus_tag="PKV23_10260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="marine proteobacterial sortase target protein"
                     /protein_id="HQX85861.1"
                     /translation="PIAKAAKGNGISKLWDRRRIADFEVASTLGTLTYDAANKAILAM
                     ALDHQIVSSQTSLVAVDKTPKRPPGEKLTRADIPLNLPAGWDYDSVFGEEMPAVEQRD
                     ARAEFLQLAMLQKPAAVPGQQQVMLPQTATPAEFMMLIAGILLVLSLAFRLALPRQSP
                     RQM"
BASE COUNT          315 a          520 c          525 g          299 t
ORIGIN      
        1 gaatgggccg gcgacggcgc ccaccaggtc accgtgccct ggcggctcat cgatgggccg
       61 gagacggcgc gagccttcgc cgaggaactg gcgggaaagc ctgctgcccg tttccgctgg
      121 ggcacttcaa tttcgtcggt tctccgcttc agcgccggac tgttcgaggg cagcggcagc
      181 cgtcaggtga tcgacatctc cggtgatggg ccaaataatg tcggcgggac tgtaaccgtg
      241 gcgcgcgatg cagtcgtggc ccagggcatc gtcatcaacg ggctgcccat gctgctcaag
      301 ccggcccagg tcctgccgcc gctcgaccgc tacttcgtcg attgcgtgat cggcggcgag
      361 ggctcattct cgctgcctgt cacctccatc gacagtttgg ccgaagccat ccgcatgaaa
      421 ctgatacgcg agcttctgtc atcggatgcg aatgacgctg gcagaatcat cccggcggca
      481 accgaacccc ccaccgactg cctcgccggc gaaaaggaac gcgcccggcg tcaggatcag
      541 tactgactat ttcaccagct ccgcatgaac cagatagcgc tctgatcccc gcgtgatggc
      601 gtcgaaggga tagcaggtgg ccagcaccag attgaagccc atggcatgcc ggtcgatgcc
      661 tgagttgttc cagtccacga ttgaggttcc ggttacccgg tagatgaaca ccaggccatc
      721 gtcgcgggtg atcgtgatgc gatcacccac cacgacatct ttcagaaagg caaaatgcgt
      781 atcccggtgc gccgaaataa ccgccgtgcc gggctcgccg atgcgggagg tttcagcaag
      841 cagcgctggg ccaaaggcca gcgcttcgcc gctgccgcca tgcagcacaa tggcgctggc
      901 ctgaatgcgg gaaatttcga tgcgtgccac cggccaggtg tcggcccagc gccaggcctt
      961 gaccagctcg cccgtcgcaa tcgtttgggt gaaggctcgt tcgagcagca cctgcgccag
     1021 ttgcgccttg gcgaatatcc atgcggattg cccgaacagg aacactgaaa gcccgaggca
     1081 aacggcgctc aggaaattga ggaaggaccg gcgcgacaag cgcaccggtc caaccttggg
     1141 cagcgtgagg gttgcgggga ccatgtcaca tctgccgtgg ggactggcgg gggagtgcca
     1201 gcctgaatgc aagactcaaa accaggagga ttccggcgat gagcatcatg aattccgccg
     1261 gcgtggcggt ctggggcagc atcacctgct gctggccagg gaccgccgcc ggcttctgca
     1321 gcatggccag ctgcaggaat tcggcccggg catcacgctg ctcaaccgcg ggcatttcct
     1381 cgccaaacac cgagtcataa tcccatcctg ccggcaggtt gagcggaatg tcggcccgcg
     1441 tcagcttttc gcccggaggc cgcttcggtg tcttgtccac cgccacaagg ctggtttggc
     1501 tggacacgat ctggtgatcg agggccatgg ccagaatggc cttgttcgcg gcatcatagg
     1561 tcagtgtgcc aagcgtcgag gccacttcga agtcggcaat ccgccgcctg tcccagagct
     1621 tggaaatgcc attgccttta gccgcctttg cgatgggaa
//
LOCUS       DAORVN010000560         1658 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_85534_length_1658_cov_3.059888, whole genome shotgun sequence.
ACCESSION   DAORVN010000560 DAORVN010000000
VERSION     DAORVN010000560.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1658
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85534_length_1658_cov_3.059888"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1519)
                     /locus_tag="PKV23_10265"
     CDS             complement(<1..1519)
                     /locus_tag="PKV23_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010911045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HQX85862.1"
                     /translation="MDRVFDFIIVGAGSAGCVLADRLSANGRHSVLVLEAGGSDRRFF
                     IQMPLGYGKTFYDPSVNWMYAAEPDPGLNGQRDYWPRGKVLGGSSSINAMVYIRGARE
                     DYESWEAAGNTGWGWDEVLRCYRAMEDTEAGGNEFRGVGGPLPVRCGPTDFHPLYLKF
                     VLASNKAGLPLNEDFNGATQEGVGAYQYTTWKGQRMSAARAFLRPAMKRRNVRVVTHA
                     QVTKVLFEGRRATGVEYLHRGVRKAALAGREVILAGGAINSVQLLQLSGIGPAKVLQA
                     QGIEVRLENANVGAHLQDHVGLNYTYRMKVPTLNDELRPWWGKLRVGLRYVLSRRGPL
                     SLSVNQGGGFFSSSPAHTRPNMQLYMQAFSTLIPKVGERPLLTPDPFSGFSLGLSNCR
                     PSSRGTIEIQSADPLQHPRIVANAYSTDEDIAEMLDAVKFLRKIAAQKPLAEVIAEEL
                     RPGPTCVSDDDLIADIRQRSGTVYHPSCTCRMGPMARDSVVDPHLRVHGIENLRVC"
BASE COUNT          337 a          548 c          443 g          330 t
ORIGIN      
        1 cacacacgcg gagattttcg atgccatgca cccgcaggtg aggatcaaca accgagtccc
       61 tggccatagg tcccatgcgg caggtgcagg acggatgata caccgtgccg ctgcgctggc
      121 ggatatcggc gatcaagtca tcatcgctta cacaagttgg tcctggccgc aattcttccg
      181 cgatcacttc agccaaaggc ttctgcgccg caatctttcg gaggaacttc actgcgtcca
      241 gcatttcagc gatatcttca tcggttgagt acgcattggc cacaatacgc ggatgctgca
      301 gcgggtcagc cgattggatt tcgatggtgc cccggctgct tggccggcag ttggaaagtc
      361 ccagggaaaa tcccgagaag ggatcaggcg tgagcagggg gcgttcaccc accttaggaa
      421 taagcgtcga gaaggcctgc atgtaaagtt gcatattggg gcgcgtgtgg gcaggcgagg
      481 agctgaagaa tccccccccc tgattgacgc tcaaactcaa tggcccgcgg cgtgacagga
      541 catagcgcaa gcccacccgc agctttcccc accagggccg cagttcgtca ttcagcgtcg
      601 gcactttcat ccggtaggtg taattcagcc ccacatggtc ctgcagatgc gcccccacat
      661 tcgcattctc cagccgcacc tcgattccct gcgcttgcag gaccttcgct ggcccgatgc
      721 cagatagctg gagcaattgc accgagttga tggccccgcc tgccagaatg acctcacgcc
      781 ctgccagcgc ggctttcctg acaccgcgat ggagatattc aacccccgtg gcgcgccgtc
      841 cctcaaacag aaccttcgtt acctgcgcat gggttacaac ccgcacattc cggcgtttca
      901 ttgcgggccg caggaaggcc cgagccgccg acatgcgctg tcctttccac gtggtgtatt
      961 gataggcgcc aaccccttcc tgcgtcgccc cgttgaaatc ttcgtttagg ggcagcccgg
     1021 ccttgttgct cgccagcaca aacttgagat agagcggatg aaaatctgta ggcccgcagc
     1081 gcaccggcag cggcccgcca acgccgcgga attcgttgcc gcccgcctcc gtgtcttcca
     1141 tggcccggta gcagcgcagc acctcatccc agccccaacc ggtgttcccc gccgcttccc
     1201 agctttcata gtcttcgcgg gcgccccgga tataaaccat ggcattgatt gagcttgagc
     1261 cccccagcac cttgccgcgc ggccagtagt cccgctggcc gttaagccca gggtccggtt
     1321 ccgccgcata catccagttt accgaaggat cataaaacgt cttgccatag cccagcggca
     1381 tctggatgaa gaagcgccgg tccgagccgc ccgcctcgag caccagcacg ctgtggcggc
     1441 cattggcgga cagacgatcc gccaggacac agcccgccga acctgcgcct acgatgataa
     1501 aatcaaaaac cctgtccacg cctcaccgtc agttagaaaa tttcatccct gcttgctcct
     1561 gcaagctgtt atcgtttagc tttatgccat tgtcttgaaa gaagcacccc catgaaaatc
     1621 accgagttga aggtctttat cgtcggcaat ccgccgcc
//
LOCUS       DAORVN010000561         1658 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_85567_length_1658_cov_2.175920, whole genome shotgun sequence.
ACCESSION   DAORVN010000561 DAORVN010000000
VERSION     DAORVN010000561.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1658
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85567_length_1658_cov_2.175920"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            163..960
                     /locus_tag="PKV23_10270"
     CDS             163..960
                     /locus_tag="PKV23_10270"
                     /inference="COORDINATES: protein motif:HMM:NF020478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity EipB family protein"
                     /protein_id="HQX85863.1"
                     /translation="MAAVFLALVSQAEASAVAPHRAVYDLQLLRATQGANVGSVSGRM
                     AYEVSGSECDGWSVSFRLVNRFDYKEGSPRLFDTQSSSWETGDGKSMTYSEKEFVDNQ
                     QEKEKRVSVVRSEKDAVGEVSITLPQVKKSSIPAETVFPMRHQLRLMEAAARGESRDV
                     SLVYDGSDEEKTVRAISTIGKKVNAGEGKKDRDNAVAAPLLSLASWPVTIGYFSSEEE
                     NAETPLYQINFEMYENGVSTGLVMDYGSFALSGKLAHLELLKAETCP"
     gene            1036..>1658
                     /locus_tag="PKV23_10275"
     CDS             1036..>1658
                     /locus_tag="PKV23_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018699120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HQX85864.1"
                     /translation="MAKSTTSITSKGETEVADFLEKVRGLPPVARDGKRGKLIFAMDA
                     TMSRQPSWDRALSIQAEMFAETARIGGLDVQLVYFRGFNECRASKWVSNPTALARLMT
                     TVDCRGGNTQIGRALSHIKKELRSTKVNAVIYVGDAMEENIDALCQAAGEIGLQGTPI
                     FTFQEGNDPNAARAFQEMAKLSRGAYFKLNESSANMLRDLLSAVAVYA"
BASE COUNT          366 a          494 c          472 g          326 t
ORIGIN      
        1 ctttccacct ctgcaggcag atgccgggtc accagaattc ttgctttggc cattgttgtc
       61 atctttgctt tgccttaacc ttcaaaagct gaaatcataa tcgacaggtg ggcaatggac
      121 caccgtaatc atggagtttc aagggtgttg aacagagttt tattggcagc cgttttcctg
      181 gcgcttgtgt cgcaggccga ggcttcggcc gtggcgcccc accgcgcggt ttacgatctg
      241 caattgctcc gcgccaccca gggcgccaat gtcggcagtg tctccggccg catggcttat
      301 gaagtctccg gcagcgaatg cgatggctgg tccgtgagtt tccgcctggt caaccgcttt
      361 gactacaagg aaggcagccc gcgcctcttc gacacccagt cctcatcctg ggaaaccggt
      421 gacggcaagt cgatgaccta cagcgaaaag gaatttgtcg ataaccagca ggagaaggaa
      481 aagcgcgtca gcgttgtccg ctccgaaaag gatgctgtgg gcgaagtcag catcaccttg
      541 ccgcaggtga agaaatcctc catcccggcg gaaacggtgt tccccatgcg ccaccagttg
      601 cgcctgatgg aagccgcggc aagaggcgag tcccgcgatg tctcgctggt ttatgacggc
      661 tccgacgagg aaaaaaccgt gcgcgccatc agcactatcg gcaagaaggt gaatgccggc
      721 gaaggcaaga aggacaggga caatgccgtg gcggccccgc ttctgtcgct ggcttcctgg
      781 cctgtcacca tcggctattt ttcatcggaa gaagaaaacg ccgaaacgcc gctctaccag
      841 atcaattttg aaatgtatga aaacggcgtc tccaccggcc tggtcatgga ttacggcagc
      901 ttcgccctct cgggaaaatt ggcccatctc gagctcctga aagctgaaac gtgcccctga
      961 gccccggcga aattgctttg aatttgcaaa aagcgcacta tctctagcgc cgctgtgcgc
     1021 gcaaggggca aggacatggc caagtcaacg acctccataa cgtcgaaggg tgagaccgag
     1081 gttgccgatt ttctggagaa agtccgcggc ctgccgccgg ttgccaggga cggcaagcgc
     1141 ggcaagctga tctttgccat ggacgccacc atgagccgcc agccgagctg ggaccgcgcc
     1201 ctttcgattc aggccgaaat gttcgccgaa accgcccgca tcggtggcct ggatgtgcag
     1261 ttggtctact tccgcggctt caacgagtgc cgcgccagca aatgggtgag caaccccacg
     1321 gcactcgcca ggctcatgac aacggtggat tgccgcggcg gcaacaccca gatcggccgg
     1381 gccctgtccc acataaaaaa ggaattgcgc agcaccaaag tgaatgccgt catctatgtg
     1441 ggcgacgcca tggaagaaaa cattgatgcc ctgtgccagg ccgccggcga aatcggcctg
     1501 cagggaaccc ccatattcac cttccaggaa ggcaacgatc ccaatgccgc gcgcgccttt
     1561 caggagatgg ccaagctcag tcgaggcgcc tatttcaagc tcaatgaatc atcggccaac
     1621 atgctgcgag atcttctctc cgccgttgct gtctatgc
//
LOCUS       DAORVN010000562         1654 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_85867_length_1654_cov_3.512195, whole genome shotgun sequence.
ACCESSION   DAORVN010000562 DAORVN010000000
VERSION     DAORVN010000562.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1654
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85867_length_1654_cov_3.512195"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..112
                     /locus_tag="PKV23_10280"
     CDS             <1..112
                     /locus_tag="PKV23_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012496244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQX85865.1"
                     /translation="RRAVLTNMHNDMDYDTLRGELPAGVEPAYDGLKLPL"
     gene            131..>1654
                     /locus_tag="PKV23_10285"
     CDS             131..>1654
                     /locus_tag="PKV23_10285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85866.1"
                     /translation="MNTKAIAAFSALMLFSSSAVAQEACDPAKLSAMVDTYAGNPFSA
                     RTWRVLNGLGDPMIEPSYSGSDTWQNQDAWKKLVAAILPPGQAVDEVGYDCRIGYPLE
                     VLQSRIATLGKDNPYVQQWLKVQGKVLQVCSNPDTIDVALPEPIEPDPAHPTMQAEDR
                     AYQEATIFFYKDKPKAIELFRAIAATASPHKAAARYNIANLLANSKDVAAARAEANAI
                     LADPALASVHNITQELLGYIANLEDTPAGWSALIDDSIKVIETPAKDILASDKLRADY
                     ARALNDIDYAGIRGKREDWWLSGTLPENPTISKAILDASRQHSMALWMMAGQSLQENY
                     DAAPWSLVGDKWQQRAASYLDQVMAVMPSGAKIPVLSLDVLNSLRAKPDDATREALWG
                     KVHGSYDAAQKSCGAAPETAALGIYLAQAVRVSAATGHFDQVYDELGKLPLQSSSFYQ
                     YNVVQKLGQYLVGQGMAAEGRKMRDRLLTPAFMSAIPASMNPGVTDGNSNFMGWVAED
                     "
BASE COUNT          352 a          485 c          509 g          308 t
ORIGIN      
        1 gaggcgggcg gttctcacca acatgcacaa tgacatggac tatgatacgc tgcgtgggga
       61 gcttccggcg ggtgttgaac ccgcctatga cgggcttaaa ctgccgttgt gattccgggg
      121 ggaccttaga atgaacacca aagcaattgc cgcattcagc gccttgatgc tgttttcaag
      181 ttcggccgtg gcgcaggagg cctgtgatcc ggccaagctt tcggccatgg ttgacaccta
      241 tgccggcaac cccttcagcg cccgcacctg gcgcgtgctg aacggccttg gcgacccgat
      301 gatcgagccg tcctacagcg gctccgacac ctggcagaac caggacgcct ggaagaagct
      361 tgttgccgcc atcttgccgc ccgggcaggc tgtcgatgaa gtcggctatg attgccgcat
      421 cggctatccg ctggaggtgc tgcaaagccg gatagcgacg ctgggcaagg acaatcccta
      481 tgtgcagcag tggctgaagg ttcaggggaa ggttctgcag gtctgcagca acccggatac
      541 gatcgacgtg gccctgccgg agccgattga acctgatccg gcgcatccca cgatgcaggc
      601 cgaggaccgg gcctaccagg aagctacgat cttcttctat aaggacaagc cgaaagccat
      661 cgaattgttc cgcgccattg ccgcgactgc ctcgccgcac aaggccgcgg cacgctacaa
      721 cattgccaac ctgttggcga attcaaagga tgtggcagct gcgcgggccg aggccaatgc
      781 catcctggct gatccggcgc ttgcctctgt tcataacatc acccaggagc ttctcggcta
      841 tatcgccaat cttgaagaca caccggcggg ctggagcgcg ctgatcgacg actccatcaa
      901 agtcatcgag acaccggcga aggacattct cgcttcagac aagttgaggg ccgattatgc
      961 ccgggcgctt aacgatatcg attatgcggg cattcgcggc aagcgcgaag actggtggct
     1021 gagcggaacc ctgccagaga atcccacgat ctcgaaagca attcttgacg catcacggca
     1081 acactccatg gcgctctgga tgatggcggg gcaatcgctg caggaaaact acgacgcagc
     1141 cccatggtct ctggtgggag acaagtggca gcaacgcgcc gccagctacc tagaccaggt
     1201 catggcggtg atgccaagcg gcgcaaagat tccagtcttg tcgctggacg tgctgaacag
     1261 cttgcgggcg aagcccgatg atgccacgcg tgaagcacta tggggcaagg tccatgggag
     1321 ctatgacgcc gcgcagaaat cctgcggagc agcccctgaa acggcggctc tgggcatcta
     1381 tctggcgcaa gccgtgcgcg tgtcggcggc aaccgggcat ttcgaccagg tatatgacga
     1441 acttggcaag ctgccgctgc agtcatcctc cttctatcag tacaatgttg tccaaaaact
     1501 tggtcagtat ctggtggggc aggggatggc tgcggaaggc cgcaagatgc gcgacaggct
     1561 gctgacgccc gccttcatgt cggcgatccc cgccagcatg aatccgggcg tgaccgacgg
     1621 caattcaaat ttcatgggtt gggtggccga ggac
//
LOCUS       DAORVN010000563         1654 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_85882_length_1654_cov_2.946842, whole genome shotgun sequence.
ACCESSION   DAORVN010000563 DAORVN010000000
VERSION     DAORVN010000563.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1654
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85882_length_1654_cov_2.946842"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /locus_tag="PKV23_10290"
     CDS             <1..215
                     /locus_tag="PKV23_10290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HQX85867.1"
                     /translation="RQALDRGLIDGIGHLHEVLKQKFGDKMILRNIAASRGWGLSRLG
                     FGAISEFPDSALAAIETRALWSKFGL"
     gene            238..414
                     /locus_tag="PKV23_10295"
     CDS             238..414
                     /locus_tag="PKV23_10295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85868.1"
                     /translation="MLFAKVVTVIIAGVFTILTARRLMNEVNAAKVRAKPRQPNTVTK
                     LRQDPSTGIYYPVE"
     gene            414..1055
                     /locus_tag="PKV23_10300"
     CDS             414..1055
                     /locus_tag="PKV23_10300"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQX85869.1"
                     /translation="MSGHSSDAWLEGVYHAKTRDDLIALYDGWADTYDADMQGIGYVH
                     PAVIAGLVGRYVPNQSDPVFDAGVGTGTVGKILAILGYSNLIGVDMSDGMLARATARR
                     IYADLRNRVLGEPLDFADGSMAAIVSTGVFTTGHGPAHAWDELTRITRAGGHLIFTVG
                     TVVWQESGFARKFDELCGKGLVEPVETTPVYHPMPYSPTESGFTTRAHVYRRL"
     gene            1137..>1654
                     /locus_tag="PKV23_10305"
     CDS             1137..>1654
                     /locus_tag="PKV23_10305"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013532824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HQX85870.1"
                     /translation="MPLNPSKSFQDLILTLHQYWAAKGCVILQPYDMEVGAGTFHPAT
                     TLRSVGPKPWAAAYVQPSRRPKDGRYGENPNRLQHYYQYQVIIKPSPPDLQELYLGSL
                     AAIGIDPKLHDIRFVEDDWESPTLGAWGLGWEVWCDGMEVSQFTYFQQVGGLDCFPVS
                     GELTYGLERLAM"
BASE COUNT          318 a          543 c          458 g          335 t
ORIGIN      
        1 gccgccaggc cctcgaccgc ggcctgatcg acggcatcgg ccacctgcat gaggtgctga
       61 agcagaagtt cggcgacaag atgattctca ggaatatcgc agcctccagg ggctggggcc
      121 ttagccgcct tggtttcggc gcgatttccg agtttcctga ttctgccctt gcggcaatcg
      181 aaacacgtgc attatggtcg aagttcggtt tatagtggtg ggaacaagga gatgacgatg
      241 ctgtttgcca aggttgtgac tgttatcatt gccggtgttt tcaccattct caccgcgcgc
      301 cgcctgatga atgaggtgaa cgccgccaag gtccgggcga agccgcgcca gcccaatacg
      361 gtcaccaagc tccgccagga tccatcaacg ggcatctact accccgttga gtgatgtcgg
      421 gtcattccag cgacgcctgg cttgaaggcg tctaccacgc caagacccga gacgatctta
      481 tcgcccttta tgacggctgg gccgatacct atgacgccga catgcagggc atcggctacg
      541 tccaccccgc ggtgattgcc ggcctcgtcg gccgctatgt tccaaaccaa tccgatccgg
      601 tcttcgatgc gggcgtcggc accggcacgg tgggcaagat cctcgccatc ctgggctaca
      661 gcaacctgat cggcgttgat atgtccgatg gcatgctggc ccgcgccaca gcccgcagga
      721 tttacgccga cctccgcaat cgcgtgctgg gtgagccgct ggatttcgcc gatggcagca
      781 tggccgccat tgtctcaacc ggcgtcttca ccaccggcca tggcccggcc catgcctggg
      841 atgaactcac gcggatcaca agggcagggg gccatctcat cttcaccgtc ggaaccgtcg
      901 tctggcagga gtcaggcttt gccaggaaat tcgacgaact ctgcggcaag ggtctggtag
      961 aacccgtcga aaccaccccg gtctatcacc ccatgcccta ttcgcccacg gaatcaggtt
     1021 tcaccacaag agcccacgtt tatcgcaggc tctaatgtct tctcccgcga gcgggagagg
     1081 accaaaacct tatctccttg acctctcaaa cccgcatccc tagtttgcgc ccgcaaatgc
     1141 cgctaaatcc gagcaaatca ttccaggacc tgattctcac cctgcaccag tattgggcag
     1201 ccaagggctg cgtgatcttg cagccctatg acatggaagt gggggccggc acctttcatc
     1261 ccgccaccac gctccgctcc gtcgggccaa agccctgggc cgcggcttac gtgcagcctt
     1321 cgcgccgccc caaggatggc cgctacggcg aaaaccccaa ccgcctgcag cactattacc
     1381 agtaccaggt gatcatcaag ccatccccgc ccgatttgca ggaactctat ctcggctcgc
     1441 ttgccgccat cggcattgat cccaagctcc acgacatccg ctttgtcgag gatgactggg
     1501 aaagccccac gctcggcgct tggggccttg gctgggaagt ctggtgcgat ggcatggaag
     1561 tctcgcagtt cacctacttc cagcaggtgg gcggcctcga ttgctttccc gtgtccggcg
     1621 aattgaccta tggcctcgaa cgcctcgcca tgta
//
LOCUS       DAORVN010000564         1652 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_86118_length_1652_cov_2.480902, whole genome shotgun sequence.
ACCESSION   DAORVN010000564 DAORVN010000000
VERSION     DAORVN010000564.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1652
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86118_length_1652_cov_2.480902"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..540)
                     /locus_tag="PKV23_10310"
     CDS             complement(<1..540)
                     /locus_tag="PKV23_10310"
                     /inference="COORDINATES: protein motif:HMM:NF015013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HQX85871.1"
                     /translation="MNLFKAAASVSAMTFLSRITGLVREIIGFNLFGFGAAMDAFQVA
                     WRIPNLLRRLFAEGAFSQAFVPIFAEYKATRTAAETRALADRVASLLSLALFFVTLLG
                     VIAAPVLVYATASGFSASAEQFDLTVQLVRITFPYIFFISLVSLSAGILNSFGSFKTP
                     AFTPVLLNLCVIAAAVWLAP"
     gene            674..964
                     /gene="rpsT"
                     /locus_tag="PKV23_10315"
     CDS             674..964
                     /gene="rpsT"
                     /locus_tag="PKV23_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019080555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HQX85872.1"
                     /translation="MANTKQAAKRARQAVKQRSHNMAQRTELRTAIKKVKAAVAAGDA
                     VQAEAVFKASTSTIDSIADKNIIHKNKAARHKSRLSAAIKAIAAKGQTAAQA"
     gene            complement(1093..1428)
                     /locus_tag="PKV23_10320"
     CDS             complement(1093..1428)
                     /locus_tag="PKV23_10320"
                     /inference="COORDINATES: protein motif:HMM:NF023534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3579 domain-containing protein"
                     /protein_id="HQX85873.1"
                     /translation="MSSTPHRIIIQGVTLKGRAFRPSDWAERLCGIMSTFGGDQQMRY
                     SRHVRPLMLDGVRCVLVDPALRDIEPRAYRFLLDFASDNELVVIDPLELNTENYCPVP
                     GSALDRLPA"
BASE COUNT          393 a          479 c          482 g          298 t
ORIGIN      
        1 cggtgcgagc cataccgccg cggcaatcac gcacagattg agcaaaaccg gcgtgaatgc
       61 aggcgtcttg aatgatccaa aactattgag gatgccggcg gacagggaga ccagcgagat
      121 aaagaaaatg tacggaaatg tgatccgcac cagttgaaca gtcaggtcaa attgctctgc
      181 gctggccgag aatcccgagg cggtcgcgta aaccagcacc ggggccgcaa tgacccccag
      241 cagggtgacg aaaaacaggg ccagcgacag caggcttgcc acccggtcgg ccagtgcgcg
      301 cgtctcggca gcggtccgcg ttgctttgta ttcggcaaag atcggcacga acgcctggct
      361 gaacgcgccc tcggcgaaca accgtcgcaa caggttgggg atgcgccatg cgacctgaaa
      421 tgcgtccatg gcggcgccaa agccaaacaa gttgaagccg atgatttcgc gtaccagccc
      481 ggtgatgcgc gacaggaacg tcatcgctga gaccgaggcg gctgccttaa acagattcat
      541 aggggctttg gcagaagctg aactggccgg caggggacaa aagaccagca aaatcagcgg
      601 gtttgctttt tttatgagac gggatataat acaaggcttc ccgcaatatt catgatttga
      661 ttctggaaaa accatggcaa acaccaaaca agccgccaag cgcgcacgtc aggcagtcaa
      721 gcagcgctcc cataatatgg ctcagcgtac cgagcttcgt accgccatca agaaagtgaa
      781 ggcagctgtt gccgccggag atgcggtaca ggcagaggcg gtattcaagg cttccacctc
      841 gacgattgat tcaatcgccg acaagaacat cattcacaaa aacaaggctg ctcgtcacaa
      901 gagccgtctt tccgctgcga tcaaggcaat agccgccaaa ggccagaccg ccgcgcaagc
      961 gtagatctgc cgtagcctca cgggcagccc atgctgcccg ttacgacaac accgtcagcc
     1021 gacgcccgtg actgggcatg accgcaaggt ccgggctggc ggttttttgt ttcttgtccc
     1081 catggtttcg cgctaagctg gcaaccgatc aagcgcactg cctggcaccg ggcaatagtt
     1141 ttccgtgttc agttccagtg gatcaatgac gaccagttca ttgtcgctgg caaaatccag
     1201 caggaaccgg taagcgcgcg gctcgatatc ccgcagcgcg ggatcgacca ggacacaacg
     1261 cacgccgtcg agcatcagcg gcctgacatg gcgcgaatag cgcatctgct ggtcaccacc
     1321 aaaggtcgac atgatgccgc acaggcgttc tgcccaatca ctaggccgaa acgcgcgccc
     1381 cttcagggtg acgccttgga tgatgattcg atgcggggtg gaagacacgt tgtatcaggt
     1441 gcgtgtggcg ggaccgggat gtcagctttg gactcctgcc tgccgtcgcg gcgcgatgcc
     1501 ggcaacgcgg caaatgcagg ttttaggcca acatgaaatt atagcctgcc cggtattcga
     1561 cccccaacgc tggctactcg ctgacagccc cggcgcgaag ggacgcgggc gatgttgcac
     1621 tgcacagttg tttgtcctat atttgccctc tt
//
LOCUS       DAORVN010000565         1644 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_86890_length_1644_cov_2.755821, whole genome shotgun sequence.
ACCESSION   DAORVN010000565 DAORVN010000000
VERSION     DAORVN010000565.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1644)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1644)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1644
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86890_length_1644_cov_2.755821"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..660
                     /locus_tag="PKV23_10325"
     CDS             <1..660
                     /locus_tag="PKV23_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020047865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQX85874.1"
                     /translation="RVLPPSRGEIYFNGKPLPRELKDRDKDTLRRIQMIYQSADTALN
                     PRQKVRDIIGRPLEFYLGLKGADRDRRVVELLESIELNESYIDRLPGEMSGGQKQRIS
                     IARALAAEPEIIICDEVTSALDQIVQEEILSLLMRLQKQTNVSYLFITHDIATVRAIS
                     DEIVVMHQGKVVQQGPKSKVLSPPFPAYTELLLSSVPEMDPDWLTGLLAKRSKKAKVS
                     A"
     gene            669..>1644
                     /locus_tag="PKV23_10330"
     CDS             669..>1644
                     /locus_tag="PKV23_10330"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: protein motif:HMM:NF013071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HQX85875.1"
                     /translation="MNRPVIMWFRQDLRTRDHAALNAAAAAGEVICLYVLDDETPGKW
                     RWGGASRWWLHHSLQSLSKNLKLVLRRGQADTVIADVIRESGAGAVYFTRDYAPWSPS
                     LERRVKDVCESAGATCHRFGGYLLHEPEAIRNGAGEPYKVYTPFSRACFAAGEPRLPA
                     AAAKPVMWQGKLQSDNLQDWNLLPKHPDWSKGFESEWRPGEAAARAGLSRFLDEALAH
                     YAEGRDRPDQAFTSRLSPHLHWGEISPAQCWVAVRGAMAQARGKLDVAGEKYLKEILW
                     REFSYSILHHRPDLPEVPFRQEFSAFLWRSDPAELQKWQQGMTGYPIVD"
BASE COUNT          320 a          518 c          488 g          318 t
ORIGIN      
        1 cgcgtgctgc cgccctcgcg cggcgaaatc tatttcaatg gcaagccgct gccgcgtgag
       61 ctcaaggatc gtgacaagga tacgctgcgc cgcatccaga tgatttacca gtcggcggac
      121 acggcgctga acccgcgcca gaaagtgcgt gacatcatcg gccggccgct tgaattctat
      181 cttggcctca agggcgccga ccgtgaccgc cgcgtggtcg aactgctcga atccattgaa
      241 cttaacgaaa gctacatcga ccgtcttccc ggcgaaatgt ccggcggcca gaagcagcgc
      301 atttccattg cccgcgccct ggcggcggag ccggagatca tcatttgcga tgaagtgacc
      361 tccgccctgg accagatcgt ccaggaggaa attctttcgc tgctcatgcg cctgcaaaag
      421 cagaccaatg tttcctacct cttcatcacc cacgacatcg ccacggtgcg ggccatttcc
      481 gatgaaatcg ttgtcatgca ccagggcaag gttgtccagc aggggccgaa gtcaaaagtc
      541 ctgtcgccgc cattcccggc ctatacggaa ttgctgctgt cctctgttcc cgaaatggat
      601 cccgactggc tgacgggcct tcttgccaag cgttcgaaga aggccaaagt cagcgcctga
      661 ttttggcgat gaacaggcct gtcatcatgt ggttccggca ggatctgcga acccgcgacc
      721 acgccgcctt gaatgccgcg gctgctgccg gggaagtcat ttgcctttat gtgcttgatg
      781 acgaaacgcc gggaaaatgg cgctggggcg gggccagccg ctggtggttg caccacagtt
      841 tgcagagcct ctcgaaaaat ttaaaactcg ttctgcggcg gggccaggcc gacacggtca
      901 tcgccgacgt gattagggag tccggggcag gcgctgtcta tttcacccgg gactatgcgc
      961 catggtcacc gtccttggaa cgccgggtaa aagatgtttg tgaaagcgcc ggggccacct
     1021 gccaccgctt tggcggctat ctcctccacg agccggaagc catccgcaac ggcgccggcg
     1081 aaccctacaa ggtctatacc ccgttctcgc gcgcctgttt tgcggcgggc gagccgcgcc
     1141 tgccggctgc cgccgcaaag cctgtgatgt ggcaaggcaa gctccaaagc gacaacctgc
     1201 aggattggaa cctgctgccc aagcacccgg actggtccaa aggctttgaa tccgaatggc
     1261 ggccaggcga ggcggccgca cgggccgggc tttcccggtt tctcgatgaa gcccttgcgc
     1321 actatgccga aggccgcgac cgcccggacc aagccttcac ctcgcgcctc tcgccccatc
     1381 tgcactgggg cgaaatcagc ccggcccaat gttgggtggc cgtgcgcggc gcgatggcgc
     1441 aggcgagagg aaagcttgac gttgccggcg aaaaatacct gaaggaaatt ctctggcggg
     1501 aattttccta ttccatcctg caccaccggc cggatctgcc cgaagtcccg tttcgccagg
     1561 agttttctgc cttcctctgg cgcagtgatc cggctgagtt gcagaaatgg cagcagggca
     1621 tgaccggcta tccaatcgtc gatg
//
LOCUS       DAORVN010000566         1641 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_87177_length_1641_cov_2.518916, whole genome shotgun sequence.
ACCESSION   DAORVN010000566 DAORVN010000000
VERSION     DAORVN010000566.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1641)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1641)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1641
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87177_length_1641_cov_2.518916"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /locus_tag="PKV23_10335"
     CDS             complement(<1..269)
                     /locus_tag="PKV23_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85876.1"
                     /translation="MAMEANSLRVGGLGICRYRLGFSSDLRHYMALKNANVESRKPMT
                     KAVQIEMAGGPEVMQLVAVDLPRPAPGEVRIRQTAIGLNYIDTYHR"
     gene            262..1449
                     /locus_tag="PKV23_10340"
     CDS             262..1449
                     /locus_tag="PKV23_10340"
                     /inference="COORDINATES: protein motif:HMM:NF005732.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HQX85877.1"
                     /translation="MAISVSPAISRIEAPPIAEAMTWVRPGQRNRGLLNLCQAVPSYA
                     PAEALQAEIARLARDPEVHLYTDIHGVPELRTALAQHMAADYRGRISPDNVSITSGCN
                     QAFCAAIMALAQRGDNVMLPSPYYFNHQMWLDMLGVEKRLVPAFAPGRTYPLPEDAAP
                     LINDRTRAIVLCSPNNPTGAIYPASIIEAFYDLAKASGIALIIDETYKDFRSSPDPLH
                     GLFARPDWQDTFVQLYSFSKVFAMTGYRVGSIIAGSKVLAEAEKILDCMAICAPQISQ
                     RAALFGLAELEDWKRGKARLMEERREALSAAFKNPALSYRLVSSGAYFAYVMHPFSRL
                     KAKAVAMRLAGEHDVLCLPGSMFGPEQDSYLRLAFANSPAEDMPLLVERLIESQGPLG
                     TTR"
     gene            complement(1451..>1641)
                     /locus_tag="PKV23_10345"
     CDS             complement(1451..>1641)
                     /locus_tag="PKV23_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006200783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85878.1"
                     /translation="YAADPVGLPSPATLEFLAAIDYALPGWARTYMAAMEKSLGKAYA
                     EPAADVRGYIAYVKSQKK"
BASE COUNT          320 a          504 c          457 g          360 t
ORIGIN      
        1 cgatgatacg tatcaatata attgagccct atggccgttt gcctgatgcg gacttctccg
       61 ggcgcaggcc tcggcaaatc cactgcgacc agttgcatca cttccgggcc ccctgccatc
      121 tcgatctgaa ctgccttcgt catgggtttt cggctttcca catttgcatt tttcaaggcc
      181 atgtaatgcc tcaaatcgct tgaaaatcca agccgatatc ggcaaatacc tagacctccc
      241 acgcgcaagg aatttgcttc catggccatt tcggtttccc cggcaatctc aaggattgaa
      301 gcgccgccca ttgccgaggc catgacttgg gtgcggcccg ggcagcgcaa ccgcgggctt
      361 ctgaacctct gccaggcggt tcccagctat gcgccagccg aagcgttgca agctgaaatt
      421 gctcgcctgg ccagggatcc agaggttcat ctttacacgg atatccacgg cgttccagag
      481 cttcgcactg ccctggcgca gcatatggcg gcggactatc gcggccgcat cagcccggac
      541 aatgtcagca tcacctcggg ctgcaaccag gcgttttgcg ccgccatcat ggcgctggcg
      601 caacgcggcg acaatgtgat gctgccaagc ccctattatt tcaaccatca aatgtggctg
      661 gatatgctgg gcgtggagaa gcgtctggtc ccggcctttg ctccggggcg cacttatccc
      721 ctgcccgaag atgcagcgcc cctcatcaat gaccggaccc gcgccatcgt cctgtgctca
      781 cctaacaatc cgacgggagc catctatccc gcatctatca ttgaagcttt ctatgacctt
      841 gcgaaggcca gcggcattgc gctgatcatt gacgaaacct acaaggactt ccgttcttca
      901 ccggaccccc tgcatgggtt gtttgccagg ccagactggc aggacacatt tgtccagctc
      961 tacagtttct ccaaggtttt cgccatgacc ggctaccggg taggctccat catcgccggc
     1021 agcaaggtcc ttgcggaagc tgagaaaatt ctggattgca tggccatttg cgcgccgcag
     1081 atctcgcaac gggccgccct gttcggattg gcggaacttg aggattggaa gcggggaaaa
     1141 gcgcgcctga tggaggagcg cagagaagcc ttgtcggcgg cattcaagaa tcccgcgcta
     1201 tcctaccgcc tggtcagttc gggggcctat ttcgcctacg tcatgcatcc gttctccagg
     1261 cttaaagcca aagccgttgc catgcggctg gctggcgaac atgacgttct ttgcctgcct
     1321 ggctcgatgt tcgggccgga acaggattcc tatctccggc ttgcctttgc aaactcaccg
     1381 gctgaggaca tgccgctgct ggtggaaaga ttgattgaaa gccagggccc actcgggacc
     1441 acccgatgaa ttattttttc tgtgacttca cataggcgat gtagccgcgc acgtcggcgg
     1501 cgggctcggc ataggcctta cccagcgatt tctccatggc cgccatatag gtcctggccc
     1561 agcccgggag ggcgtaatct attgcggcca ggaactcgag ggtggcgggg gagggtagcc
     1621 ccaccggatc ggccgcatag c
//
LOCUS       DAORVN010000567         1639 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_87330_length_1639_cov_3.120581, whole genome shotgun sequence.
ACCESSION   DAORVN010000567 DAORVN010000000
VERSION     DAORVN010000567.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87330_length_1639_cov_3.120581"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1639)
                     /locus_tag="PKV23_10350"
     CDS             complement(<1..>1639)
                     /locus_tag="PKV23_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003612501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate synthase central domain-containing
                     protein"
                     /protein_id="HQX85879.1"
                     /translation="PSSREEGLKVLGWRDVPVDSSVLGYSVKGTEPFHRQVFIGRGPT
                     IRDEAHFERKLFVCRKVVSNRVLEVLGAKARTYYPVSVSSRTIVYKGLVLGKDLGAYY
                     KDLTDPRIESALALVHQRFSTNTFPSWPLSHPYRFVCHNGEINTVRGNYNWMAARQAN
                     MKSTVLGEDLSKLWPISYEGQSDTACFDNALEFLTMGGYSLPHAMMMLIPEAWAGNPL
                     MDEDRRAFYEHHAALMEPWDGPAAMTFTDGRLIGATLDRNGLRPARYLTTDDGFVLLA
                     SEMGCLQFAEEHVTHKWRLQPGKMLLIDLEQKRIISDEELKKTLSTAHPYKEWLKRTQ
                     VVLRDLPKARTKRPKILVPLLDRQQAFGYSQEDLRILMAPMAQTGQEAVGSMGNDAPI
                     SVLSDRAKMLDTYFKQNFAQVTNPPIDPIREELVMSLVSFIGPRPNLLDLAGTSTHMR
                     LEVSQPILTNEDLERIRTIGKISENPFRTATIDITYDVANGPEYMEAALDSVCRLAER
                     AVRDGYNIIILSDRAVSGERVPLPALLATSATHHYLIR"
BASE COUNT          308 a          482 c          530 g          319 t
ORIGIN      
        1 cggatcagat aatgatgcgt ggccgaggtg gcgagaaggg ccggcaacgg cacccgctcg
       61 cccgacacgg cgcggtccga caggatgatg atgttgtagc catccctgac ggcccgttcg
      121 gcaagccggc agacgctgtc gagcgctgct tccatgtatt ccggcccgtt ggccacgtca
      181 taggtgatgt cgatggtggc cgtgcggaac gggttttcgc tgatcttgcc gatggtgcgg
      241 atgcgctcga ggtcttcatt ggtgaggatg ggctgcgaga cctctagccg catatgcgtc
      301 gaggtgcctg cgaggtccag aaggtttggc cgcgggccga tgaacgacac cagcgacatc
      361 accagttctt cgcggatggg atcgatcggc ggattggtca cctgcgcgaa gttctgcttg
      421 aaataggtat ccagcatctt ggcccggtcc gaaagaaccg agatgggcgc gtcattgccc
      481 attgagccca ccgcctcctg ccccgtctgc gccatgggcg ccatcaggat tctcaggtct
      541 tcctgcgaat agccaaaagc ctgctgccgg tcgagcaagg gcacgaggat ctttggccgc
      601 ttggtcctgg ccttgggcag gtcacgcagc acaacctggg tgcgcttcag ccactccttg
      661 tagggatgtg ccgtggacag cgtcttcttt aattcctcgt ctgatatgat gcgcttctgc
      721 tcaaggtcga tgagcagcat cttgccgggc tgcagccgcc acttgtgggt gacatgttct
      781 tcggcaaatt gcaggcagcc catttcagag gcaagcagca caaagccatc gtcggtcgtg
      841 agatagcggg ccgggcgcaa gccgttgcgg tcaagcgtgg cgccgatcag gcggccatcg
      901 gtgaaggtca tggcggcggg gccatcccac ggttccatga gcgccgcatg gtgttcataa
      961 aaggcccggc ggtcctcgtc catcagcgga ttgccggccc aggcttcggg gatcagcatc
     1021 atcatggcgt gcggcagcga atagccgccc atggtgagga actcgagcgc gttgtcaaag
     1081 caggccgtgt ccgattgtcc ttcataggaa atgggccaga gcttcgacag gtcttcgcca
     1141 agcaccgtgg acttcatgtt ggcctggcgc gccgccatcc agttgtaatt gccgcgcacc
     1201 gtgttgatct cgccgttgtg gcagacaaag cggtaggggt gcgacagcgg ccagctcggg
     1261 aaggtattgg tggaaaaacg ctgatgcacc agggcgagcg ccgattcaat gcgcgggtcc
     1321 gtcaggtcct tgtaataggc gccgaggtcc ttgccgagca cgaggccctt gtaaacgatg
     1381 gtgcggctgg aaaccgacac cggataatag gtccgggcct tggcgccgag aacttccagc
     1441 acccggtttg acacaacctt gcggcagaca aaaagcttgc gctcgaaatg cgcctcatcc
     1501 cggatcgtcg gcccgcgccc gatgaatacc tgccggtgga agggctccgt gcctttcacc
     1561 gaatagccga gcaccgaaga gtcgaccggc acatcgcgcc agcccagaac cttcaggcct
     1621 tcctcgcgcg aggaaggcc
//
LOCUS       DAORVN010000568         1639 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_87347_length_1639_cov_2.772096, whole genome shotgun sequence.
ACCESSION   DAORVN010000568 DAORVN010000000
VERSION     DAORVN010000568.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87347_length_1639_cov_2.772096"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(59..826)
                     /locus_tag="PKV23_10355"
     CDS             complement(59..826)
                     /locus_tag="PKV23_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006206576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQX85880.1"
                     /translation="MPQRVMITAAAAGIGRAIAKAFYDEGAKVHICDVNEQALAAFRE
                     EFPEIAATHVNVANEAEVDAWFDEALEDLGGLDVLVNNAGIKGPTAPIDDVEFADWKE
                     CLAICLDSHFLCARRAAPVMKAQGSGNIINLSSTAGQYGFGNRTPYASAKWAVIGLTK
                     SLAIELGPHGVRCNAVCPGAVRGDRINRVIQGEAEIRGVAFDVIAREMTWSQSIPRFV
                     EPEEIADMCLFLASPAAKMVNGQDIAVDGHLETMHIR"
     gene            complement(828..>1639)
                     /locus_tag="PKV23_10360"
     CDS             complement(828..>1639)
                     /locus_tag="PKV23_10360"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HQX85881.1"
                     /translation="RWAFMGSFLTYHVAGGPGGMRHFISQFDPTLELPWTDLPFPKWN
                     DTLQNRLIDGCEAQAAGQTVAQIEAKRNDVLVDMMRLFKHHKVGAGLVLARDEAKQGT
                     KARHWKKSDKPDGPVKLHKGEVLSAWLDYNGHMTDAAYLIAFGDGLDAFFRYIGDDEA
                     YRATGNTFFTAETHVNYYKEMKLGEAFSIETQVLGCDEKRMHLFHRMYHGKTKELVAT
                     NEIMQLHVNQKAGKVVPMRSDLYDALYAIGKAHKKLKKPKEMGSVMKVKRK"
BASE COUNT          300 a          449 c          508 g          382 t
ORIGIN      
        1 cacgcgtcgt cttgatgatc atgtcctgtt cttcggagag cttgaaatcc atgtggggtt
       61 acctgatgtg catggtttcg aggtggccat ctacggcgat atcttggccg ttgaccatct
      121 tggcggcggg gctggcgagg aagaggcaca tgtcggctat ctcttccggt tcgacaaagc
      181 gcggaatgga ctgggaccag gtcatttccc tggcgatcac atcgaaggca acgccgcgga
      241 tttcggcctc gccctggatg acgcggttga tgcggtcgcc gcgcacggcg ccgggacaga
      301 cggcattgca gcgcacgcca tgggggccaa gttcaatggc cagtgatttg gtgaggccga
      361 tgacggccca tttggcggag gcataggggg tgcggttgcc aaagccgtat tggccggcgg
      421 tggatgaaag attgatgatg ttgcccgacc cttgcgcctt catcacagga gcggcacgtc
      481 tggcgcagag gaaatgcgag tcaaggcaga ttgcgaggca ttctttccag tcggcgaatt
      541 ccacatcgtc aatgggcgcc gttggccctt tgatgccggc attgttcacc agcacatcga
      601 gtccgcccag atcttccaac gcttcgtcga accaggcgtc gacctcggcc tcattggcca
      661 cattcacatg ggttgcggcg atctcgggga attcctcgcg gaaggcggcg agcgcctgct
      721 cattgacatc gcagatatgg accttggcgc cttcatcata aaaggctttg gcaatggcgc
      781 ggccaatccc tgcggctgcg gcggtgatca tgacgcgctg gggcatgtta tttccttttg
      841 actttcatga cgctgcccat ttccttgggc ttcttcagct tcttgtgggc ttttccaata
      901 gcgtacagcg catcgtacag gtcactgcgc atcgggacaa cttttccggc cttctggttg
      961 acatgcaatt gcatgatctc gttggtggcc acaagttcct tggtcttgcc gtggtacatg
     1021 cggtggaaca gatgcatgcg cttttcatcg cagcccagca cctgggtttc gatggagaaa
     1081 gcctcgccga gcttcatttc cttgtagtaa ttcacgtggg tttcggcggt gaagaaggtg
     1141 ttgccggttg ccctgtaggc ttcgtcgtca ccgatgtagc ggaagaaggc atcgaggcca
     1201 tcaccgaaag cgatgagata ggcggcatcg gtcatgtggc cgttgtagtc gagccaggcg
     1261 gaaagaacct cacccttgtg aagtttcacc gggccatcgg gcttgtcgga ttttttccag
     1321 tgcctggcct tggtcccctg tttggcttca tcacgggcca ggaccagtcc ggcgcccacc
     1381 ttgtggtgct tgaagaggcg catcatgtcc accagcacat cattgcgctt ggcttcgatc
     1441 tgcgccacgg tctggcccgc ggcctgcgcc tcgcagccgt caatcaaacg gttttgcagc
     1501 gtgtcattcc atttgggaaa tggcaggtcg gtccatggca gttccagggt gggatcgaac
     1561 tgcgaaatga agtggcgcat gccgccgggg ccgcctgcca catgataagt gagaaaggag
     1621 cccatgaagg cccagcgca
//
LOCUS       DAORVN010000569         1635 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_87759_length_1635_cov_2.622152, whole genome shotgun sequence.
ACCESSION   DAORVN010000569 DAORVN010000000
VERSION     DAORVN010000569.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1635)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1635)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1635
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87759_length_1635_cov_2.622152"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..795
                     /locus_tag="PKV23_10365"
     CDS             <1..795
                     /locus_tag="PKV23_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018855592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HQX85882.1"
                     /translation="DIWWGGTGDPHLQAAEEGLTEPYESPMLAKLHPWAIKQHETGKG
                     ATVGVYLGALGYGYNSELLEQKKIAAPACWADLIKPEFKGEVQVANPNSSGTAYTFLA
                     TMVQILGEEEAFKYMKALDGNINQYTKSGAAPAQAAARGETLVGIAFQHDVITPTITS
                     NAPIKNVSPCEGTGFEIGSMSIIKGAPNMENAKKWYDWALTPEAQMLGAAANSYQNPS
                     NMEAPIPDAAPKMADIKLIDYDFAKFGSSAERKRLLSRWDAEIGAK"
     gene            842..>1635
                     /locus_tag="PKV23_10370"
     CDS             842..>1635
                     /locus_tag="PKV23_10370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85883.1"
                     /translation="MAELSKQSRHILLLAIVGLIGAIALPWYVVPSGFWNFAWTGQLG
                     EASAAPLFLQALLHGRWQLLPIGLCLLVVLIELFRGRGPRGTVVAAAGGVGLFWMFAQ
                     GLLIGLRGWNANLFEQAFGPIAAQAGFGVGAVICGVALLLIFSSGLALNGLMRGDGFL
                     VSSIAVVVSCIGVFVFYPVFLVLIESAITPEGSYSLTAFLERVFSADVGQVTLNSLAL
                     GIATGLTTTLLGLGLALLHKRTDFRAKGLLRLLAMASLSLMFVVVK"
BASE COUNT          314 a          467 c          506 g          348 t
ORIGIN      
        1 gacatctggt ggggcggcac gggtgatccc catctgcagg ccgccgaaga gggactgacg
       61 gaaccctatg aatcgcccat gcttgcgaag cttcatccct gggcgatcaa acagcatgaa
      121 acaggaaagg gagcgactgt aggcgtctat ctcggggcgc tgggttacgg ctataacagc
      181 gagttgctgg agcaaaaaaa gatcgcagca cccgcctgct gggccgatct catcaagccg
      241 gaattcaagg gcgaagttca ggtcgcaaat ccaaactcat cgggcacggc ttacaccttc
      301 cttgccacga tggtgcagat cctcggcgaa gaggaagcct tcaaatacat gaaggcgctc
      361 gacggcaaca tcaaccagta cacaaagtcc ggcgcggcac cggcccaggc cgcggcgcgc
      421 ggtgagaccc tggtcggcat cgccttccag catgatgtga tcactcccac catcacgtca
      481 aatgcaccga tcaagaatgt gagcccctgt gaaggaactg gattcgagat cggctccatg
      541 agcatcatca agggtgcgcc caatatggaa aacgccaaga aatggtatga ctgggcgctg
      601 acgcctgaag cccagatgct tggcgcggcg gcgaacagct accagaaccc atccaacatg
      661 gaggcgccaa ttccggacgc ggctccgaaa atggcggaca tcaagctgat cgattatgat
      721 tttgccaagt tcggctcttc cgccgagcgc aagcgcctgc tgtcgcgctg ggatgctgaa
      781 atcggagcca aatgacatcg gatagggatc cagcggccta gcgccgccgg atcccataac
      841 catggcagag ctttccaaac aatcgcgcca tattttgctg ctggccatcg tcggcctgat
      901 aggggccatc gccttgccgt ggtatgtggt gccttcaggc ttttggaact tcgcctggac
      961 cgggcagttg ggcgaggcga gtgccgcgcc cctgtttctt caggcattgt tgcacggcag
     1021 gtggcagctc ctgcccattg ggctttgcct gctggttgtg ctcattgaac ttttccgggg
     1081 tagggggccg cgtggaacgg tcgttgccgc ggcgggtggc gttggcctgt tctggatgtt
     1141 tgcgcaggga ctcctcatcg ggctgagagg ctggaacgcc aacctgtttg aacaggcctt
     1201 cgggccgatt gccgcgcagg cggggtttgg cgtgggcgcg gtcatttgcg gcgtcgcatt
     1261 acttctcatc ttttcgtccg gcctggcgct gaacggcttg atgcggggcg atggctttct
     1321 cgtcagctcg attgcggtgg tggtgtcgtg catcggcgtg tttgtgtttt atccggtctt
     1381 tcttgtcctc attgaatctg ccatcacgcc ggaaggcagc tactcactga cggcattttt
     1441 ggaacgggtg ttttccgccg atgtcgggca agtcaccctg aacagcctgg cgcttggcat
     1501 tgccacgggg ctgacaacaa ccctgcttgg cctcggcctc gcacttctgc acaagcgcac
     1561 cgatttccgc gccaaaggcc tgctgcggct gctggcgatg gccagcctgt cgctgatgtt
     1621 cgtggtggtc aaata
//
LOCUS       DAORVN010000570         1634 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_87821_length_1634_cov_3.336289, whole genome shotgun sequence.
ACCESSION   DAORVN010000570 DAORVN010000000
VERSION     DAORVN010000570.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1634)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1634)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1634
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87821_length_1634_cov_3.336289"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..520
                     /locus_tag="PKV23_10375"
     CDS             <1..520
                     /locus_tag="PKV23_10375"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HQX85884.1"
                     /translation="RIAMRLVAGNSAELISKLLTFAIDIAVTAEHPQNESIGSFKLRE
                     DRLVVVGPKVGALLKGPRVSLAALIAAPLVLREEGSTTRRLLLEELQKRNLSPRNTIE
                     VEGREAVIESVAQGLGVAVMSRGEISPDGRLRILEFSDWPVKMEEWLLFLRARSELHL
                     IRSFLDIARPRP"
     gene            complement(517..1404)
                     /locus_tag="PKV23_10380"
     CDS             complement(517..1404)
                     /locus_tag="PKV23_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014332845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQX85885.1"
                     /translation="MTTHLRVLRPAPKVFAFYDGRVDGYRFAEGENWVDDGALSLGIA
                     SYAIVDGADALVYDTHVTVQHAAFIRKTLADAGVKNFTVVLSHWHLDHVAGSEAFDDC
                     RIISNKRTLDHLSRRKAAIESGEYSGPPVINPLVLPTQTFEGRMQLQVGKLRVELLEA
                     NIHSDDATVLWLAPERLLLAGDTLEDTVTYVGEPENFDIHLAELDRLWALRPERILPN
                     HGDPEIIALGGYEKTFIRANQQYIRMLKRCVSEPELRTKPLRDIISAPLEAGWVNLFA
                     PYEAIHRQNIEVVLAAKLS"
     gene            1436..>1634
                     /locus_tag="PKV23_10385"
     CDS             1436..>1634
                     /locus_tag="PKV23_10385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85886.1"
                     /translation="MAEKTCLANWPEYCDNRCLGHSGETEMTFGYGLLALIVAIAGYL
                     VYAYNALVSNKNLLPEGWSGIH"
BASE COUNT          333 a          459 c          480 g          362 t
ORIGIN      
        1 gcgcatcgcc atgaggctgg tggccggaaa ttccgccgag ttgatttcaa agcttctgac
       61 attcgcaatt gacatcgctg taaccgccga acatccccag aatgagtcca tcggaagttt
      121 caagctgcgc gaagacaggc ttgttgtcgt agggccaaag gtgggcgcat tgctaaaggg
      181 ccccagggta tcgctcgccg ctttgatcgc cgcgccgctt gtgctgcgtg aggaaggctc
      241 cacaacgcgg agattgctgc tggaagagct gcagaagcgc aatctgagcc cgcggaacac
      301 gattgaggtc gaaggccgtg aagccgtcat tgaatccgtg gctcagggcc ttggcgttgc
      361 cgtcatgtcc cgcggcgaga tttctcctga tggccggttg cgcattcttg agtttagcga
      421 ctggcctgtg aaaatggagg aatggctgct tttcctgcgc gcccggtcgg agctgcatct
      481 catcaggagt tttcttgata ttgccaggcc gcggccctag ctcagttttg cggccaggac
      541 cacctcaatg ttctgccggt ggatggcttc atagggggcg aagagattga cccatcccgc
      601 ctccagcggc gcgctgatga tgtcccgcaa gggtttcgtc cgcagctccg gctcgctcac
      661 gcagcgcttg agcatgcgga tatactgctg gttggcgcgg ataaaggtct tttcatagcc
      721 gccaagcgca atgatttccg gatcgccatg attgggcagg atgcgctccg gcctcaatgc
      781 ccagagtcgg tccagttcgg ccaggtggat gtcgaaattt tccggttcgc ccacataggt
      841 caccgtatcc tcgagtgtat cgccggcaag caggagccgc tccggcgcga gccacagaac
      901 cgttgcatca tcgctgtgga tgttggcctc aagcagttcg actctgagct tgcccacctg
      961 caattgcatg cggccttcaa aagtctgggt tggcaggaca agtggattga tcaccggcgg
     1021 tccggaatac tcgccgcttt cgatggcggc cttgcggcgg ctcagatgat ccaaggtgcg
     1081 cttgtttgaa atgatcctgc aatcgtcaaa ggcctcactt cccgccacat gatcgagatg
     1141 ccagtggctg agcaccacgg tgaaattttt cacgccggca tcggctagcg ttttgcggat
     1201 gaaggcggcg tgttgcactg tcacgtgcgt gtcatagaca agcgcatccg cgccatcgac
     1261 gattgcataa ctggcaatgc ccaacgaaag cgcgccgtca tctacccagt tctcgccctc
     1321 ggcaaagcgg tagccatcga cccggccatc gtagaaagca aaaacttttg gggcagggcg
     1381 taggacccgc aagtgcgttg tcatcggtat tctcccattc cggcggagca gccttatggc
     1441 agaaaaaacc tgtctggcaa actggcccga atactgcgat aacaggtgcc ttggccactc
     1501 aggggaaaca gaaatgacat ttggctatgg tctcttagcg ctcatcgtcg ccatcgcagg
     1561 ctatttggtt tatgcttata acgcacttgt cagcaacaag aaccttctgc ccgaaggctg
     1621 gagcggaatt cacg
//
LOCUS       DAORVN010000571         1628 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_88391_length_1628_cov_3.092816, whole genome shotgun sequence.
ACCESSION   DAORVN010000571 DAORVN010000000
VERSION     DAORVN010000571.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1628)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1628)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1628
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88391_length_1628_cov_3.092816"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            227..1444
                     /locus_tag="PKV23_10390"
     CDS             227..1444
                     /locus_tag="PKV23_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010686516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQX85887.1"
                     /translation="MNAVSTTQDSPYAWFRLAVSLALATIGGIGIWSGIVVLPFIEVE
                     FGVDRSGASFPYTMSMIGFALGGLLMGRLADRFGIMVPMFLGSVMLGLGFVTSALAST
                     YEIYVASQAVLIGLGCATIFGPLVADVSLWFQKRRGIAVAVAASGNYIAGTIWPPFLT
                     WGIQTYGWRYAYIALGILTVSLMLPLSLFLRRRATLDERPSPSITATGGGKALDGTPV
                     LQGFLVLAGLACCVAMSMPQVHIIAYCGDLGYGPARGAEMLSIMLGFGVVSRLAFGFI
                     ADRIGGVKTLIIGSFLQCLALLFYIPFDGLASLYIVSAFFGLAQGGIVPSYALIVRDY
                     FPAREAGFRISLVLTATVLGMALGGWLSGEIFDRTGSYQAAFLHGIAWNMLNMAIAFW
                     LLMGRKTPKLRPA"
BASE COUNT          243 a          569 c          450 g          366 t
ORIGIN      
        1 cggtgaacgc cgacgaccca tccttccgcg aaaaccgcct gaagcttttg aaccgcatcc
       61 gtgcggcgac cctggcggtg gcggatttct cgaagattga aggctaattt tttcctctcc
      121 ccgatggggc gagggaaata gtctttcagc ctctcggcac aacagcaata tcagccatgc
      181 accgccccat cttgcccaat caatgccggc ggtgagataa ggccccatga acgccgtctc
      241 caccactcaa gacagcccct atgcctggtt ccgccttgcc gtctccctgg cgcttgccac
      301 cattggcggc attggcatct ggtccggcat tgttgtcctg cccttcatcg aggtggaatt
      361 cggcgttgac cgctcgggcg cgtcgttccc ctacaccatg agcatgatcg gctttgccct
      421 cggcggcttg ctgatgggaa gactcgccga ccgtttcggc atcatggtgc ccatgttcct
      481 gggctccgtc atgctggggc tgggttttgt cacctccgcc cttgcctcca cctatgaaat
      541 ctatgttgct tcccaggcgg tgctcatcgg cctgggctgc gccacgattt tcgggccgct
      601 ggttgctgac gtctcgctct ggttccagaa gcgccgcggc atcgcggtcg ccgttgccgc
      661 atccggaaac tacatcgctg gcaccatctg gccgcccttc ctcacctggg gcatccagac
      721 ctacggctgg cgctatgcct atattgcgct gggcatcctc accgtatcgc tcatgctgcc
      781 cctgtcgctg tttctgcgcc gccgcgccac gctggatgaa cggccaagtc cctccatcac
      841 cgcgaccggt ggcggcaagg ccctcgacgg aacccccgtg ctgcagggct ttctcgtcct
      901 agcgggcctt gcctgttgcg tggccatgtc catgccccag gtccacatca tcgcctattg
      961 cggcgatttg ggttacggcc cggcgcgtgg cgcagaaatg ctctccatca tgctgggctt
     1021 cggcgtggtg agccgccttg ccttcggctt tatcgccgac cgcatcggcg gcgtgaaaac
     1081 cctcatcatc ggctccttcc tgcaatgcct ggcgctgctg ttctacatcc ccttcgatgg
     1141 cctggcctcg ctttacatcg tctccgcctt ctttggcctc gcacagggcg gcatcgtgcc
     1201 aagctatgcc ctcatcgtgc gcgactattt ccccgcccgt gaggcaggct tccgcatcag
     1261 cctggtgctc accgcaaccg tattggggat ggcgctgggc ggctggctct ccggcgaaat
     1321 cttcgaccgc accggctcct accaggccgc cttcctccac ggcattgcct ggaacatgct
     1381 taacatggcc atagctttct ggctgcttat gggacggaaa accccaaaac tccggcctgc
     1441 ctaaaatctg gccacttgac ctgccgccgt gcagcgtgtt tgctgcactg caatatgccg
     1501 atgaaagcca tagtcgcgtt ggggaaaacc ccgccatcgc tgggggcgaa ggcggctggc
     1561 ctcggcgaca tgctggccct aggcctcgcc gtgcccccgg gcttcgtcat caccgaccct
     1621 tccggcct
//
LOCUS       DAORVN010000572         1622 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_88973_length_1622_cov_3.873006, whole genome shotgun sequence.
ACCESSION   DAORVN010000572 DAORVN010000000
VERSION     DAORVN010000572.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1622
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88973_length_1622_cov_3.873006"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..676)
                     /locus_tag="PKV23_10395"
     CDS             complement(<1..676)
                     /locus_tag="PKV23_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HQX85888.1"
                     /translation="MAKPRTLYDKIWDDHLVHEADDGTCLLYIDRHLVHEVTSPQAFE
                     GLRMTGRKVRHPERTLAVVDHNVPTTDRSKGIAEEESRIQVETLAQNARDFGVEYFHE
                     RDKRQGIVHVVGPEQGFTLPGTTIVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQTL
                     IQKKAKNMLVDVSGKLAPSATAKDIILSIIGEIGTAGGTGSVIEFAGEAIRDLSMEGR
                     MTVCNMT"
     gene            complement(766..1584)
                     /locus_tag="PKV23_10400"
     CDS             complement(766..1584)
                     /locus_tag="PKV23_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008834541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HQX85889.1"
                     /translation="MTDYLKTNLANWNDRAAIHIKDEAGGYRVREFLAGEDNLHGIED
                     HEIGDVRGKRIAHLQCHFGIDTLCLARRGATCVGLDFSPVAIAAARDLQKQTGLAASF
                     VEGNVYDARELIEGHFDMVYVTWGAIGWLPDIARWARVVSSLLKPGGTLYLLEGHPSL
                     MALNEKTPNLEHGFDWRTPQDVPIVEEETTTYTGDLTPVAAATTNSWIHPLSDIINAV
                     IGAGLRLDYLHEHEELAWQFAPIMVAKEGKRRMWVLPEGFKKLPLAFSLQATKS"
BASE COUNT          295 a          446 c          529 g          352 t
ORIGIN      
        1 tggtcatgtt gcagacagtc atgcggcctt ccatgctgag atcacggatg gcttctccgg
       61 caaattcgat gaccgagccg gtgccgccgg cggtgccgat ttcgccgatg atagagagga
      121 tgatgtcctt ggcggtggcg ctgggggcga gcttgccgct cacatccacc agcatgttct
      181 tggccttttt ctggatgagc gtctgggtgg cgagcacatg ttccacttcc gaggtgccga
      241 taccatgggc cagggcgccg aaggcgccat gggtggaggt atggctgtcg ccacagacga
      301 tggtcgtgcc gggaagggtg aagccctgtt caggccctac cacatggacg atgccctggc
      361 gcttgtcgcg ctcatggaaa tactcgacgc cgaaatccct ggcgttctgg gccagggttt
      421 caacctggat gcggctttcc tcttcggcga tgcccttgct gcggtcggtg gtgggcacgt
      481 tgtgatcgac aacggccagg gtgcgctcgg gatggcgcac cttgcggccg gtcatgcgca
      541 ggccttcgaa agcctggggg gaggttacct catggacgag atgccggtcg atatagagca
      601 ggcaggtgcc gtcatcggcc tcgtggacga gatggtcgtc ccagatcttg tcataaagtg
      661 tgcgcggctt ggccatttgc aatctcgaaa tgaaagttgt ggccgctatt tagcccttgc
      721 tgccgacatg tcaaatgccg gttcctgcat ggggctttgc aggattcagg actttgttgc
      781 ctgaagcgag aaggcgaggg ggagcttctt gaagccttcg ggaagaaccc acatgcggcg
      841 cttgccttct ttcgccacca tgatgggggc gaattgccag gcgagttctt cgtgttcatg
      901 gagatagtcg aggcggagcc ccgcaccgat cacggcgttg atgatgtcgc tcagggggtg
      961 aatccacgag ttcgttgttg cagcggcaac gggcgttaaa tcaccggtat aggttgtcgt
     1021 ctcctcctcg acaatgggca cgtcctgcgg ggtgcgccag tcaaagccgt gctcgaggtt
     1081 tggggttttt tcattgagtg ccatgagcga gggatggcct tccagcaagt acagtgtgcc
     1141 gccaggtttc agcagcgagg acacgaccct ggcccagcgc gcgatgtccg gcagccagcc
     1201 gatggcgccc caggtgacat aaaccatgtc aaagtggccc tcgatcagtt cgcgggcatc
     1261 ataaacattg ccctcaacga agctggcagc aagtccggtc tgcttctgca gatcacgggc
     1321 ggcggcaatg gcaacgggcg agaagtccag ccccacacag gtggcgcccc gccttgcaag
     1381 gcaaagcgtg tcgatgccga aatggcactg cagatgggcg atgcgctttc cccggacatc
     1441 accgatctca tggtcttcga tgccgtgaag attgtcttcg cccgccagga attccctgac
     1501 gcggtagccg cccgcctcat ccttgatgtg gatggcggcg cggtcgttcc agttggcgag
     1561 attggttttg agataatcgg tcatggctta tgctccataa aaaaggccgg catcgctgcc
     1621 gg
//
LOCUS       DAORVN010000573         1619 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_89311_length_1619_cov_2.686061, whole genome shotgun sequence.
ACCESSION   DAORVN010000573 DAORVN010000000
VERSION     DAORVN010000573.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1619)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1619)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1619
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89311_length_1619_cov_2.686061"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1116)
                     /gene="rnd"
                     /locus_tag="PKV23_10405"
     CDS             complement(<1..1116)
                     /gene="rnd"
                     /locus_tag="PKV23_10405"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009764540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - endoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HQX85890.1"
                     /translation="MDLITDTKALTALCKALAHETFVTVDTEFMRETTYWPDLCLIQI
                     AGAEINGLIDPMAEGIDLKPFFALMNNEKVLKVFHAARQDVEIMVHLAGTVPHPIFDT
                     QIAAMVCGFGDQVSYEAIVRKLAKAQIDKSSRFTDWSRRPLTEKQLTYALSDVTHLRT
                     VYEKLKIELDANGREPWLAQEMAILMAIETYRTEPGDAWKRIKVRLKSKKQLAVLIAV
                     AGWRELEAQEKNVPRSRVIKDDAVAEVAMQMPQSKEALGQLRALPRGMATSRTGDGLL
                     RAVKDGLGRDPLTLPHANWGREESSESTQAAAEILKLALKVVCEREGLAPKLVANTSD
                     IEAVAESDEADVPLMHGWRREVFGNLALELNRGQAMIG"
     gene            complement(1218..>1619)
                     /locus_tag="PKV23_10410"
     CDS             complement(1218..>1619)
                     /locus_tag="PKV23_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007602341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HQX85891.1"
                     /translation="WHERDISHSSVERGIGPDATVHLDFALKRLAGVIDKLLVYPENM
                     QRNLDRLGGLVHSQRILLALTQKGMSREASYAAVQRNAMPVWRGEGNFLDLLKKDKEV
                     SAHLRPAELEALFDLGYHTKHVDTIFKRVFS"
BASE COUNT          310 a          525 c          454 g          330 t
ORIGIN      
        1 gccgatcatc gcttggccgc gattcagctc cagcgccagg ttgccgaaca cttcgcgccg
       61 ccagccgtgc atcaggggca catcggcctc atcgctttcc gccaccgctt caatatccga
      121 ggtgttcgcc acaagcttcg gcgccagccc ctcgcgctcg caaaccactt tcagcgcaag
      181 cttcaaaatc tccgccgccg cctgggttga ttcagagctt tcctcgcggc cccaattggc
      241 gtggggcagg gtgaggggat cgcgccccag gccgtccttg acggcgcgca gcaggccgtc
      301 gccggtccgc gaagtggcca tgccccgcgg cagcgccctg agttgcccaa gggcttcctt
      361 gctttgcggc atctgcatgg caacttccgc caccgcatca tccttgatca cccgcgaccg
      421 cggcacgttt ttttcctgcg cctccaattc gcgccacccg gcaactgcga tgagaaccgc
      481 aagctgcttc ttggacttga gccgcacctt gatgcgcttc caggcgtccc cgggttccgt
      541 ccggtaggtt tcaatcgcca tcaggatcgc catctcctgg gccagccacg gctcgcggcc
      601 attggcatcg agttcgatct tcagcttttc atagaccgtg cgcagatgcg tgacatccga
      661 cagcgcatag gtcagctgct tctcggtcaa ggggcggcgc gaccagtcgg tgaagcgcga
      721 tgacttgtcg atctgcgcct tggccagctt gcgcacgatg gcctcgtagc tcacctggtc
      781 gccgaagccg caaaccatgg cggcaatctg cgtatcgaaa atcggatgcg gcacggtgcc
      841 ggcaagatgc accatgatct ccacatcctg ccgcgccgca tggaaaacct tcagcacctt
      901 ctcgttgttc atgagggcga agaagggctt gaggtcaatg ccttccgcca tcggatcgat
      961 cagcccgttt atctccgccc cggcgatctg gatgaggcag agatccggcc aataggtcgt
     1021 ctcgcgcatg aattccgtgt caacggtcac aaaagtctcg tgcgccaacg ccttgcacag
     1081 cgccgtcagc gcctttgtat cagtaatgag gtccatgcgg gttaactcgc cagagtcgag
     1141 aggcaaggtc aacctcacga aggggattta ttttaaataa acaatatcaa tacgttatag
     1201 agaattacgg agtcaaatta tgagaacacc cgcttgaaga tcgtgtccac gtgcttggtg
     1261 tgatagccga ggtcgaacag ggcttcgagc tcggccggcc tgagatgcgc cgaaacttcc
     1321 ttgtccttct tcagaagatc aagaaaattg ccttcgcccc gccacaccgg catggcgttg
     1381 cgctgcaccg ccgcatagga cgcctcgcgg ctcatgccct tttgcgtcag cgccagcaga
     1441 atgcgctgcg aatgaaccag tccgcccagc cggtccaggt tgcgctgcat gttttccgga
     1501 tagaccagca acttgtcgat gacgcccgcc aggcgcttga gggcaaaatc cagatgcacc
     1561 gtggcgtccg ggccgatgcc gcgctccacc gatgagtggg aaatatcgcg ctcgtgcca
//
LOCUS       DAORVN010000574         1618 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_89407_length_1618_cov_3.013436, whole genome shotgun sequence.
ACCESSION   DAORVN010000574 DAORVN010000000
VERSION     DAORVN010000574.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1618
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89407_length_1618_cov_3.013436"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(29..715)
                     /locus_tag="PKV23_10415"
     CDS             complement(29..715)
                     /locus_tag="PKV23_10415"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HQX85892.1"
                     /translation="MKKQAKKKATTRPRDAATLILVRFDGQHPRILMGKRHGAHAFMP
                     HKYVFPGGRCERADWHLPVVNEGEPATLQKLKLRMGARPSHRKARGLLVAALRETFEE
                     TGLLAGRGTTSEALDLSGVVYFARAITPPGRSRRFDSRFFVADASIISNLDAPHALEN
                     EELLQVNWVSLPEALGLNLPSITRDILLLLQPYLTQGALPPADCPVSFQYSRGKAWLI
                     EELTLTFGPG"
     gene            complement(672..1094)
                     /locus_tag="PKV23_10420"
     CDS             complement(672..1094)
                     /locus_tag="PKV23_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF983 domain-containing protein"
                     /protein_id="HQX85893.1"
                     /translation="MEAGHIEIADRSWTRAMWRGALGKCPACGRGALFRKFMKVSDAC
                     PHCGEALHHQRADDAPPYFTIFIVGHILVPLVLIVERVWQPSYLVHAILWLPLTLALT
                     LALMPIVKGAVVGLQWALGMHGFGFEKTGEEKSHDQTA"
     gene            complement(1165..>1618)
                     /locus_tag="PKV23_10425"
     CDS             complement(1165..>1618)
                     /locus_tag="PKV23_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014130773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HQX85894.1"
                     /translation="DISRLTKTAEMISDAERRAMAAERETTDRLIAAHLAGQTGANFR
                     GRIGGVVGAGLFIKLNDTGADGFVPVSSLGSDYFVYSDVTHSLTGQRTGETFQLGDQV
                     EVKLLEVTPVSGGLRFEMVSEGRKGKPPPRHGRGPRLSKQTPKSRKKR"
BASE COUNT          320 a          526 c          484 g          288 t
ORIGIN      
        1 tccggagggg atttgagggc gatagaccct atcccgggcc gaaagtcaag gtgagctcct
       61 cgatcagcca ggccttgccc cggctgtact ggaaggagac cgggcagtcc gccggaggca
      121 atgccccttg cgtgagatag ggctgaagca gcagcaggat atcgcgggtg atcgagggca
      181 ggttgagccc cagggcttcc ggaaggctta cccaattaac ttgcagaagc tcctcatttt
      241 caagcgcatg aggcgcgtca agattcgaaa tgatgctggc atcggccacg aagaagcggg
      301 aatcaaaacg ccggctccgt cccggcggcg tgatcgcccg ggcaaaataa accacgccgg
      361 aaagatcaag cgcctcgctc gtcgttccgc gccccgcaag caatccggtt tcctcgaaag
      421 tttcccgcag cgccgccacc agaagtcccc gcgccttgcg gtggctcggc cttgccccca
      481 tccgcaattt gagtttctgc aaagttgcag gctcgccttc attcactacc ggcaaatgcc
      541 agtcggccct ctcgcagcgt ccgccgggaa acacatattt atgcggcatg aaggcatggg
      601 ccccatggcg cttgcccatg aggatgcgtg ggtgctggcc atcgaagcgc acgaggataa
      661 gggttgccgc gtcacgcggt ctggtcgtgg cttttttctt cgcctgtttt ttcaaagcca
      721 aagccgtgca tccccagggc ccattgcagg ccgacaaccg ccccttttac gatgggcatg
      781 agcgccagtg tcaaggcaag tgtcaggggc agccagagaa tggcatggac gaggtaggac
      841 ggctgccaca cccgctccac gataagcacc agcggaacca agatatggcc gacgatgaag
      901 atggtgaaat agggcggcgc atcatcggcc cgctggtggt gcagcgcctc gccgcaatgc
      961 gggcaggcat cgctaacctt catgaatttc cggaacaggg caccgcggcc gcaggccggg
     1021 cattttccca aggccccgcg ccacatggcc cgcgtccacg agcggtcggc aatctcgatg
     1081 tgtccagctt ccatgccccc aaggtgggcg tcaatggacc gttagggtag ggcaccccgc
     1141 gccgcgcggc ggcttgccgc aagcctagcg tttcttccgc gatttcggcg tctgcttgct
     1201 caggcgtgga ccccgcccat ggcgcggcgg cggcttgccc ttgcgacctt cggaaaccat
     1261 ctcgaaacgc aggcctcccg ataccggcgt gacctcaagc agtttcactt ccacctggtc
     1321 cccaagctgg aaagtctcgc ccgtgcgctg cccggtcagg gaatgggtca catccgaata
     1381 cacgaaataa tccgagccca ggctcgagac cggaacaaag ccatcggccc ccgtgtcatt
     1441 gagcttgatg aaaagtcccg cccccaccac gccgccgatc cgcccccgga aattggcacc
     1501 cgtctgtccg gcaaggtgcg cggcaatcag gcgatcggtt gtctcgcgtt ccgccgccat
     1561 cgcccgccgc tccgcatcag aaatcatctc ggcggttttg gtgagccgcg aaatatca
//
LOCUS       DAORVN010000575         1616 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_89622_length_1616_cov_2.903908, whole genome shotgun sequence.
ACCESSION   DAORVN010000575 DAORVN010000000
VERSION     DAORVN010000575.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1616
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89622_length_1616_cov_2.903908"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="PKV23_10430"
     CDS             <1..235
                     /locus_tag="PKV23_10430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85895.1"
                     /translation="LPTALKYGITKYGISVPLRDGDFGDVLFSVNVKSTDDKWALQRE
                     DIVAKFRPFAVYFHGRAKPLIQSRKFAEINFAA"
     gene            complement(232..1509)
                     /locus_tag="PKV23_10435"
     CDS             complement(232..1509)
                     /locus_tag="PKV23_10435"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012111732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HQX85896.1"
                     /translation="MPAQPTAPGFCRDCFSAVEPQDMRCPRCHGPRLKRHAELHELAI
                     AHIDCDAFYAAVEKRDNPDLKDKPVIIGGGTRGVVSTACYVARIKGVKSAMPMFKALA
                     LCPEATVIRPDIRKYSAVGKEVRELMLELTPQVEPISIDEAFLDLTGTTRLHGKSPAL
                     SLATLLKAIETRIGITASAGLSYNKYLAKVASDLEKPRGYSVIGKAEAKDFLAEKSVS
                     LIWGVGRAFQAQLLRDGISLIGQLQKMERGELMRRYGVMGARLYHLSRGEDARHVSPH
                     DESKSISAETTFNQDISSFEELERILWQLAEKVSWRAKKDGLSGLTVVLKLKSTDFKI
                     RSRNTTLDEPTQLAHRIFSAAVPMLKREATGTAFRLIGVGISQLSPAVAETASLDARV
                     ETLTKAERAIDKLRGKFGRHAVERGIGFKRDSD"
BASE COUNT          306 a          477 c          486 g          347 t
ORIGIN      
        1 gctgcccacc gcgctcaaat atggaatcac caaatacggc atttcggttc ccctcaggga
       61 tggcgatttt ggcgatgtgc tgttttcagt caacgtcaag agcaccgatg acaagtgggc
      121 actgcagcgc gaggacattg tggcgaagtt ccgccccttt gccgtgtatt tccatggccg
      181 ggcaaagccg ctcatccagt cgcggaaatt cgccgaaatc aatttcgccg cctaatcgct
      241 gtcacgcttg aagcctatcc cgcgttcgac cgcatggcgg ccgaattttc cgcgcagctt
      301 gtcgatggcg cgctccgctt tggtcagggt ttccacgcgc gcatcaaggc tcgcagtttc
      361 ggccacggcc gggctcagct gcgaaatgcc tacgccaatc aaacgaaatg ccgtgccggt
      421 cgcctcgcgc ttgagcatgg gaacggccgc cgagaaaatg cggtgggcca attgggtggg
      481 ctcatccaac gtggtgttgc ggctgcggat cttgaaatcg gttgatttga gcttcaagac
      541 cactgtgagg cccgacagcc cgtctttctt ggcgcgccac gaaacctttt cggcaagctg
      601 ccagagaatg cgttcgagct cttcaaagct gctgatgtct tggttgaagg tggtttcggc
      661 cgagatgctc ttggattcat catggggcga aacatggcgg gcgtcttcgc cacgcgacag
      721 atgataaagc cttgccccca tcacgccgta gcgccgcatg agctcgccac gctccatttt
      781 ctgcagctgg ccgatgaggc ttatgccatc cctgaggagt tgcgcctgga aggcccggcc
      841 cactccccag atcaggctca cggatttctc agccagaaaa tctttcgcct cggccttccc
      901 gattactgaa tagccccttg gcttctccag gtcggaggca accttggcga ggtatttgtt
      961 ataggaaagc ccggccgagg cggtaatgcc gatcctcgtt tcaatggcct tgagcagggt
     1021 tgccagcgaa agcgccgggc ttttgccatg cagcctcgtg gtccccgtca aatccaggaa
     1081 tgcctcgtca atggagatgg gctcgacctg cggggtgagt tccagcatga gttcacgcac
     1141 ctccttgccc acggcggaat acttccggat atcggggcgg atcaccgttg cctcgggaca
     1201 gagggccagc gccttgaaca tgggcatggc ggatttgacc cccttgatgc gggccacata
     1261 gcaggcggtg gagacaacgc cccttgtgcc gccgccgata atgacgggct tgtccttcag
     1321 gtccggattg tcgcgtttct ccaccgccgc atagaaagca tcgcaatcga tatgggcgat
     1381 tgccagttcg tggagttcgg catggcgctt caggcgcggg ccatggcagc gggggcagcg
     1441 catatcctgc ggttcgactg cggaaaagca gtcacggcag aatccggggg ctgtcggttg
     1501 cgcaggcatt ccggcagttt agcgccgaat tccgttgcgc gctaacgccc tgaaatcaaa
     1561 tccagcacgt gcgggtgcac ttcttcccag gtgtgggtgc gcagcgagac gaaatc
//
LOCUS       DAORVN010000576         1615 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_89722_length_1615_cov_2.814744, whole genome shotgun sequence.
ACCESSION   DAORVN010000576 DAORVN010000000
VERSION     DAORVN010000576.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1615
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89722_length_1615_cov_2.814744"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(97..504)
                     /locus_tag="PKV23_10440"
     CDS             complement(97..504)
                     /locus_tag="PKV23_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013844704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85897.1"
                     /translation="MALQYSTAVRNARLDVVESTIGTSAVLKIRSGSVPANCGTADSG
                     TVLATCTLPSDWMAAASGGTKSLSGTWQDTSADATGTAAHFRIYASDGTTCGMQGTVT
                     ATGGGGDMTVDNVSFAAAQAFTVTSFTLTAGNA"
     gene            complement(514..777)
                     /locus_tag="PKV23_10445"
     CDS             complement(514..777)
                     /locus_tag="PKV23_10445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85898.1"
                     /translation="MADNLNTQRMADITTFGIAATSHTPAATDLPGNVRAVVFDAAGT
                     MSFKNSTGGSTVTGFPVLSGVPLPFIPVQITAMTGPTTCFLIT"
     gene            complement(1075..>1615)
                     /locus_tag="PKV23_10450"
     CDS             complement(1075..>1615)
                     /locus_tag="PKV23_10450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85899.1"
                     /translation="TRTKRRDGWVDWIVLRCSGKSTLRLAHALDGHHVKTWTPAAIIR
                     KRKPRSTLINEQRVPLMPSFVFAGSQHVETLLAWSHRPPDDCPQFSLFRFDRRIPLIA
                     DLSMTGLRREEERQNLITRKAKARRFETGETVAPTDGAWQGMVGQVVKAKGREYLVCF
                     SSNMTVTIADWLLEAKAAA"
BASE COUNT          333 a          447 c          485 g          350 t
ORIGIN      
        1 ccagaattct gttgcgcgaa tcaaacgtag caggattaga acttggcggc tcgttggcct
       61 gcgggataaa taccgcgaga gtatcgccgg aggccattac gcattaccgg cagtaagggt
      121 gaagctggtg acagtaaacg cctgcgccgc cgcaaatgaa acgttatcca ccgtcatatc
      181 gccgccgccg ccggtcgcgg taacggtccc ttgcattccg caagtcgtgc cgtcgctggc
      241 ataaatacgg aagtgggcag cggtgccggt cgcgtctgct gatgtatcct gccacgtccc
      301 agaaagtgat tttgtgccgc cagacgccgc agccatccag tccgatggta gcgtgcaagt
      361 cgcaagcacg gtgccgctgt cggcagtgcc gcagttagca ggcaccgatc ccgatctgat
      421 cttgagaacg gcgctcgtgc cgatggtgga ttccaccaca tcaaggcgcg cattcctaac
      481 cgcagtcgaa tactggagag ccatcgccgg tccttaagta atcaaaaaac aggttgtcgg
      541 cccggtcatg gcggttatct ggaccggaat gaatgggagc gggacgcccg aaagaaccgg
      601 aaagccagtc accgttgagc cgccggttga gtttttgaag gacattgtgc cagcggcatc
      661 gaaaacaacc gcgcgcacat tgccgggaag atcggttgca gccggagtgt ggcttgtcgc
      721 agcaataccg aatgtcgtta tatctgccat ccgctgcgtg tttagattgt cggccatgtc
      781 attctcctga cgaatttacc cgccctcaat ttcccctaca cttagccagc gttagcctgt
      841 gtgttgtgcg gtgcgatcgg ggcgggagcg cggcaaaggg agcaccgcgc aaagctgatt
      901 tattttgtgc aagatacgct atccggcaaa agcgggacag cccgattgat gaaagcgcga
      961 ggggagcggg ctggttaggt tctaggcttg gcgccggttt gccagttcac gcacccttgc
     1021 tgacttccgc agggactttg accccatatc gtattattcg ggtttttgca ataactaagc
     1081 ggcggccttt gcctcgagca gccaatcggc aattgtcacc gtcatgttcg agctgaaaca
     1141 taccagatat tcgcggccct ttgccttgac cacttggcca accatgcctt gccatgcgcc
     1201 atcggtcggg gcaacggttt cgcccgtttc aaagcggcgg gccttggctt tgcgcgtgat
     1261 aaggttctgg cgttcttcct cccgtcgcaa tcctgtcatg gatagatcgg caatcagggg
     1321 gatgcggcgg tcaaagcgaa acagcgagaa ttgcgggcaa tcgtcgggcg ggcggtgcga
     1381 ccatgccagg agcgtctcaa catgctgcga accggcaaag acgaagctag gcattaaagg
     1441 aacccgctgt tcgtttatca gtgttgagcg gggcttgcgc ttgcggatga tggccgccgg
     1501 cgtccatgtc ttgacatgat ggccgtcaag cgcgtgggcg agccgtagcg ttgacttgcc
     1561 ggagcagcga agcactatcc aatccaccca tccatccctt cgttttgttc gggtt
//
LOCUS       DAORVN010000577         1610 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_90272_length_1610_cov_2.153698, whole genome shotgun sequence.
ACCESSION   DAORVN010000577 DAORVN010000000
VERSION     DAORVN010000577.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1610)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1610)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1610
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90272_length_1610_cov_2.153698"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..667)
                     /locus_tag="PKV23_10455"
     CDS             complement(<1..667)
                     /locus_tag="PKV23_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008127812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HQX85900.1"
                     /translation="MKKFRWGLIGASTIGREWVIDAMRQAGGEIASVFSTDSQRGASY
                     ASEFGIPRSTTDLNDLLNSPIDAVYISTTNDLHAHQCIAAAKAGKHILCEKPLATNVA
                     DARSMVRICKSANVVLATNHHLRGAATHRAMRDAVKAGKIGRPLAIKVVHAGYLPVHL
                     QGWRLTNPAAGAGAILDLTVHDADLMRFIINDDAARVSATVQNSGMAIAGVEDAAMGV
                     ITFR"
     gene            746..>1610
                     /locus_tag="PKV23_10460"
     CDS             746..>1610
                     /locus_tag="PKV23_10460"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HQX85901.1"
                     /translation="MSHMAREAAEAPEAVARFLQHNGKALREIGARLRRQPPPVIITS
                     ARGSSDCAAGYFKYLAEILAGIPCSSMGASVVSVYGAKLQLKNGLCVTVSQSGKSPDI
                     VALQDAARKAGALTVALVNVEDSAAAHAADICLSLQAGPELSVAATKSFIVSLVAGAA
                     IVAHWLDRKDLLAAIEALPGQLATTARIDWPEAVDLATGAESLYVVARGPSLPIATET
                     ALKPKETCAIHAEAYSIAEVMHGPLELLGKGFPVLAYSPEDGARQSSHISLGKMRQAG
                     ANVLVAEEGGLP"
BASE COUNT          320 a          458 c          479 g          353 t
ORIGIN      
        1 agcggaaagt gatcacgccc atggccgcat cctccacacc ggcgattgcc atgccgctgt
       61 tctgcaccgt tgccgaaact cttgccgcat cgtcgttgat gatgaaacgc atgagatcgg
      121 cgtcatgaac agtgaggtcg aggattgctc ccgcgcccgc cgccgggttg gtcaggcgcc
      181 agccctgcag gtgaaccggc aggtagcccg cgtggacgac ctttatcgca agcggcctgc
      241 cgatttttcc ggctttgacg gcatcccgca tggcacgatg ggtcgcagct ccccgcaaat
      301 gatggttggt tgcgagcacg acattggctg acttgcaaat cctcaccatg ctccttgcat
      361 ctgccacatt ggttgcaagc ggcttttcgc agagaatatg tttgcctgcc ttggctgcgg
      421 caatgcactg gtgggcgtgt aaatcattgg ttgtggaaat gtatacggca tcaataggcg
      481 aatttagaag gtcattgaga tcggttgttg agcgcggaat tccgaattca cttgcatatg
      541 aggcaccccg ctgtgaatct gtgctgaaaa cgctcgcaat ctctccgccg gcctggcgca
      601 tcgcatctat cacccattcc cggccaatgg tggatgctcc aataagtccc cagcgaaatt
      661 ttttcattga tttcgcttac caagtaaatc aggctcaagc aaggtttggg cgagcaagaa
      721 gaagtcccca aggagcagat aagacatgag ccatatggcg cgtgaagccg ctgaggcgcc
      781 ggaagcggtc gcccgtttcc ttcagcacaa tggcaaagcg ctccgggaga ttggcgcccg
      841 tctccggcgc cagccgcccc ccgtcatcat cacatcggcg cgcggcagtt ctgactgcgc
      901 tgccggctat tttaagtatt tggctgagat cctggccgga attccctgtt cgtcaatggg
      961 cgcatcggtt gtgtcggttt atggcgcaaa gcttcaattg aaaaacgggc tgtgtgtaac
     1021 tgtctcccaa tccggtaaaa gccctgacat tgtggcgctc caggacgcgg cccggaaggc
     1081 aggagctttg acggtggcgc tggtcaatgt ggaagactcg gctgcggccc atgctgcgga
     1141 tatttgcttg tccctccagg ctggcccgga attgagcgtc gcggcgacga agtctttcat
     1201 tgtctcgttg gtggcggggg cggcaatcgt ggcgcattgg cttgatcgca aggacctgct
     1261 tgccgcaatc gaagcgctgc ccggccaact ggcaacgaca gcaagaattg attggcccga
     1321 agcggttgat ctcgccacgg gcgcagagtc gctttacgta gtggcgcgtg gaccatcgct
     1381 gcccatcgcc acggaaacgg ccctgaagcc gaaggaaacc tgtgccatcc acgcagaagc
     1441 ttattccatc gcggaagtca tgcatggccc cctcgaactt ctcggcaaag ggtttccggt
     1501 tcttgcctat tcacctgagg acggggcacg ccagtcttct cacatatcgc tgggcaagat
     1561 gcgccaggct ggagccaatg ttctggtggc agaggagggc gggctgccct
//
LOCUS       DAORVN010000578         1609 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_90324_length_1609_cov_3.310167, whole genome shotgun sequence.
ACCESSION   DAORVN010000578 DAORVN010000000
VERSION     DAORVN010000578.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1609
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90324_length_1609_cov_3.310167"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(273..1229)
                     /locus_tag="PKV23_10465"
     CDS             complement(273..1229)
                     /locus_tag="PKV23_10465"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR02925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpsD family peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HQX85902.1"
                     /translation="MTIKTITPFIMLPVILAITMSGCSKKDGDKAATQVVAKVNGAEI
                     SLHQVNHVLSKAGNVPADQLERARKTAVDRLIDQELLSAKAVEEKLDRTPETLMAIEL
                     ARREVLARARMQQVLAAAPKLTPGDVTKYYVTHPELFGERQVYSLQEITFPPQKAGVA
                     DVQALVDKGASMNEIQELLRARNVQFAANAGIRAAEQLPMDMLKVLHGTREGGILLSN
                     SPQGHTVVKVVGIRKVPVDEATARPMIQTFLFNQRAAEATANELKSLKATAKIEYVGS
                     LADLPKPDASTRQGQGAASTNLPALPQLDQMPINTGDNNAKK"
     gene            complement(1288..>1609)
                     /locus_tag="PKV23_10470"
     CDS             complement(1288..>1609)
                     /locus_tag="PKV23_10470"
                     /inference="COORDINATES: protein motif:HMM:TIGR02914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="EpsI family protein"
                     /protein_id="HQX85903.1"
                     /translation="KESVRSELVLRQGSIPVKQLVAQRLQRREPLTYWAIIGDKVATS
                     AADAKLTGLSYGLRGTIPDGIIFRVSTTGLPDDVAFATQREFVQDLFDSLPSASRKRL
                     AGLP"
BASE COUNT          290 a          479 c          480 g          360 t
ORIGIN      
        1 ctcctgagtc ctgatagctg cgcgtttcga gcggtaagca cggcgacagt gcatatccgt
       61 gggctgatct gatcctcaag cgcgaaggct cgtttgaccc gaagatcgaa ccggagttga
      121 agcggctgag gcagaagtat ttttacgaaa cttagcccgc gggaaggctt tcattgtcat
      181 ggagtcgaca gcgactcagc tcgagaagca cccgcccgtt caacggagag gagcatatcc
      241 ggtacccctt tggccctctt gagccccatg gattacttct tcgcattgtt gtccccggtg
      301 ttgattggca tctggtcgag ttggggcaga gcgggcaggt ttgtcgaagc ggcaccctga
      361 ccctggcgtg tcgatgcatc aggcttcggc aagtcggcaa gcgatccgac gtactcgatc
      421 ttggctgtcg ccttcaagct tttcaactcg ttggccgtcg cttccgcggc gcgctgattg
      481 aacaggaacg tctggatcat gggtcttgcg gtcgcctcat cgaccggcac ttttcggatc
      541 ccgaccacct ttacgacggt atggccttgc gggctgttgc tcagcaatat cccgccctcc
      601 ctggtgccat gcagcacctt gagcatgtcc atcggcaact gctccgctgc ccgaatgccg
      661 gcattggcgg caaactgcac gttgcgagca cgcagcaatt cctggatttc attcatggac
      721 gcacctttgt cgaccagggc ctgcacatcg gccacgcccg ccttttgcgg cgggaacgtg
      781 atttcctgga ggctatagac ctgccgctcg ccgaacagct ccgggtgggt gacgtagtac
      841 ttcgtcacat ccccgggagt cagcttgggg gcagcagcca ggacctgctg catgcgcgcg
      901 cgcgccagta cctcgcgacg ggccagttcg atggccatca gcgtttccgg cgtgcgatcc
      961 agcttctcct cgaccgcctt ggcggagagc agttcctggt cgatcagacg gtcgactgcg
     1021 gtcttgcgcg cccgctccaa ctgatccgcg ggaacgttcc ccgccttgga caggacatgg
     1081 ttgacttgat gcagggaaat ctcggctcca ttcaccttgg cgacgacttg cgtcgccgcc
     1141 ttgtcgccat ccttcttgct gcatccagac atagtgattg caaggatcac cggaagcatg
     1201 atgaaagggg taatggtttt gatcgtcatg gtgcattcct cggtcagatt aatcaggatg
     1261 gattggcgaa aggctgtcgc atatcagtca gggcaggccc gccagtcgtt tgcggcttgc
     1321 gctgggaagt gaatcaaaaa gatcttgcac gaactcccgc tgcgtggcga aggccacgtc
     1381 atccggcaaa ccggtggtcg atacgcggaa gatgatcccg tcaggaatgg ttccccgcaa
     1441 gccgtatgat aggcccgtca gtttggcatc ggcggcgctg gtcgcaacct tgtcgccgat
     1501 gatggcccag taggtcagcg gttcccgcct ctggaggcgc tgcgccacca gttgctttac
     1561 cggtatcgat ccctgccgga gtaccaattc gctgcgcacg gattccttg
//
LOCUS       DAORVN010000579         1594 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_91844_length_1594_cov_2.820013, whole genome shotgun sequence.
ACCESSION   DAORVN010000579 DAORVN010000000
VERSION     DAORVN010000579.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1594
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91844_length_1594_cov_2.820013"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            68..1540
                     /locus_tag="PKV23_10475"
     CDS             68..1540
                     /locus_tag="PKV23_10475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85904.1"
                     /translation="MITVGQQIAGGLLIGNAILVLLSALLIPADPKLGPMFEPSRSFI
                     PAALDVLIGASLLSGKVKYRAWAIIWVVLGTILFTALAWTKDPVLAVSQVMIGGSLLL
                     LLLGNAGRPRLVAGGALFFVYALLNVSGLSATVLGVNPLGPVFQTVLGDIEPRSAGQV
                     TGQGSHYRVQAASSRWHLRKDAVAKKTNPLADRWLTRPDLDAHVLIISEKVVGKQIAP
                     DALTDVVIENAKKAASSFEVISREPMQRHPKIGRIVHSRSVVDNLELETLSGVIGTYE
                     HGYQLIAFAPRKSFPKVEAELRSILESFALPTDEPPGLPDDVEPGVVRQIIGQKQPYQ
                     LSAPQGNWYLRKDAVAKQGNELSDRWIVRPELDAHVMIIAELVEGAELDPDHYATAVA
                     NSLVANVGAKILSREPVASDPQHGRLLHVTATVDGLPIEYSYGCFAKGEQAFQVMAFS
                     HQDQYPELAEDFKKAIESFRFPPPTHRPAITGPRVRKPAR"
BASE COUNT          266 a          470 c          532 g          326 t
ORIGIN      
        1 ttcaacgccc gcttctgccc ctccacctgc ctctgcccct gtccccggcg ccgctgaccc
       61 ccaagacatg attactgttg gccagcagat tgccggcgga ctgctgatcg gcaatgccat
      121 cctggtgctg ctgagcgcgc tgctgatccc ggctgatccc aagctggggc cgatgttcga
      181 gccatcccgg agcttcattc cagcggcgct tgacgtgctg attggggcgt cacttttgtc
      241 ggggaaggtc aagtatcggg cgtgggcgat catttgggtg gtgctgggga cgatcctgtt
      301 tacggcgctg gcctggacca aagatccggt cttggcggtg tcgcaggtga tgatcggcgg
      361 ctcgctgctg ctgctgcttt tgggcaacgc ggggcggcct cggctggtgg cgggtggggc
      421 gctgttcttt gtctatgcgc tgctgaatgt ctcggggctt agcgccacgg tgctgggggt
      481 caatccgctg gggccagtgt ttcagacggt gcttggcgac atcgagccga ggtcagcagg
      541 gcaggtgact ggacaggggt cgcactatcg ggtgcaggcg gcatcgtcgc gctggcacct
      601 gcgcaaggat gcggtggcga agaagaccaa tccgctggcg gatcgctggc tgaccaggcc
      661 ggatcttgat gcgcacgtgc tcatcatctc ggaaaaggtg gtgggcaagc agattgcgcc
      721 cgatgcgctg accgatgtgg tgatcgaaaa tgccaagaag gcggcgtcgt cgttcgaggt
      781 gatcagccgt gagccgatgc agcgccatcc caagatcggt cggatcgtgc attcgcggag
      841 tgtggtcgat aacctggagc tggagacgct gagtggagtg attggcacct acgagcatgg
      901 ctatcagctg attgcctttg cgccgcgcaa gtcgtttccc aaggtcgaag cagagctgcg
      961 tagcatcctc gagtcgtttg cgcttcccac cgatgagcct ccgggccttc cggacgatgt
     1021 tgagccgggc gtggtgcgtc agatcatcgg tcaaaagcag ccctatcagc tgtctgcgcc
     1081 gcaaggaaac tggtatctgc gcaaagatgc ggtggcgaaa cagggcaacg agctgtccga
     1141 ccgctggatc gtgcggcccg agctggatgc ccacgtgatg atcatcgccg agctggtgga
     1201 aggggcagag cttgacccgg atcactatgc aacggcggtt gcgaactctc ttgtcgcgaa
     1261 tgtgggggcg aagatcctat cgcgtgagcc ggtggcttcg gacccgcagc atgggcgctt
     1321 gctgcacgtc acggccacgg tggatggact cccgatcgag tactcctatg gctgctttgc
     1381 caagggagag caggcgttcc aggtgatggc gttctcgcac caagatcagt atccggagct
     1441 ggccgaggac ttcaaaaaag cgatcgagtc gttccgcttc cctccgccca cgcaccgccc
     1501 agccatcacg ggtccgcgcg tccgcaagcc cgcccgctag ctttcgcttc gtccatcatt
     1561 ccgtgctccc gcccgcatcc gcagttcccc caac
//
LOCUS       DAORVN010000580         1593 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_91949_length_1593_cov_3.197009, whole genome shotgun sequence.
ACCESSION   DAORVN010000580 DAORVN010000000
VERSION     DAORVN010000580.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1593)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1593)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1593
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91949_length_1593_cov_3.197009"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..310)
                     /locus_tag="PKV23_10480"
     CDS             complement(<1..310)
                     /locus_tag="PKV23_10480"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HQX85905.1"
                     /translation="MTIFDFITRAEPAAIAIAAPDRPPMTYEALAGLIGKTVKSLNGA
                     GLGRTDKVAIVLPNGPLMATAFLAVSACCITAPLNPAFREDEFRYYMEDLNIKALIVD
                     S"
     gene            391..>1593
                     /locus_tag="PKV23_10485"
     CDS             391..>1593
                     /locus_tag="PKV23_10485"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018184993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HQX85906.1"
                     /translation="MTDRLVIALAQLNPTLGAIQANMAKARATCLDAAGADLVLFPEL
                     FICGYPPEDLVLRPSFVAQCKVALEELAKDTKNGPAILMGTPWVEGGKLYNAMALLKD
                     GKIETLRFKYDLPNYGVFDEKRVFQAGPAPGPISFKGVRIGVPICEDIWTDETCETLA
                     ETGAEILLVPNGSPFEGNKDDVRLNLVVARVTETGLPMAYLNQVCGQDELVFDGASFV
                     LNADRSLAVQMPMFVEDIALTEWTRGADGWRVTPGAIAKLPEGEEETWKACVLGLKDY
                     VEKNRFPGVVLGLSGGIDSAVVAAMAVDALGKERVHCVMLPYAYTSKDSLVDAEACAK
                     LLDVRYDVVPIKEPVEGFLSTLKPLFEGKNSDVTEENIQSRARGVILMAISNKFGYMV
                     LTTGNKSEM"
BASE COUNT          320 a          421 c          521 g          331 t
ORIGIN      
        1 cgctatccac gatcagcgcc ttgatgttca gatcttccat atagtagcgg aattcgtctt
       61 cgcggaaggc agggttgagc ggagccgtga tgcagcaggc tgaaacagcc agaaaggccg
      121 tggccatcaa cggtccattg ggcaggacaa tggccacctt gtcggtgcgg ccaagccccg
      181 ctccgttaag gctcttaacg gtcttgccga tgagcccggc cagagcctca taagtcatgg
      241 gggggcggtc cggagcggca attgcaatgg ctgcgggctc agcccgggtg atgaaatcga
      301 atatggtcat gccccactct tggtgggttt tctgaaaacc gtcaaccgcc agcaattgaa
      361 gacaggggtt ctatccgcta tgaggcgggc atgaccgaca gactcgtcat tgcgctggcc
      421 cagttgaacc cgacgctggg tgccattcag gccaatatgg ccaaggcaag ggctacctgc
      481 ctggacgcgg caggggctga ccttgtactg tttccggagc tctttatctg cggctatccg
      541 ccggaagacc tggttttgcg ccccagtttc gtggcccagt gcaaggtagc gctagaggaa
      601 ctggccaagg atacaaagaa tggcccggcc attctcatgg gcacgccctg ggttgaaggc
      661 ggcaagctgt ataatgcgat ggcgcttctc aaggatggaa agatcgagac cctgcgcttc
      721 aaatatgact tgcccaatta tggcgtgttt gacgagaagc gggtgtttca ggcgggccct
      781 gcccccggcc ccatttcctt caagggggtg cggatcggcg ttcctatctg cgaggatatc
      841 tggaccgatg aaacctgcga gaccctggcg gaaaccgggg ctgaaatcct gctggttccc
      901 aatggttcgc cgttcgaggg caacaaggat gatgtgcggc tcaatctggt ggtggcgcgg
      961 gtcaccgaga cgggcctgcc catggcctat ctcaatcagg tgtgcgggca ggatgaactg
     1021 gtgtttgatg gcgcctcatt cgttctgaat gcggatcgct ctctggcggt gcaaatgccg
     1081 atgttcgtgg aggacatcgc gctcacggaa tggacgcgcg gggcggatgg ctggcgggtg
     1141 acaccggggg ccatcgccaa gcttccggag ggcgaggaag aaacctggaa ggcctgcgtg
     1201 ctgggcctca aggattatgt ggagaagaac cgcttccccg gcgtggtgct cggcctttcc
     1261 ggcggcatcg actcggctgt ggttgcagcc atggcggttg atgcgctggg caaggagcgg
     1321 gtgcattgcg tgatgctgcc ttatgcctat acgagcaagg acagcctggt tgacgccgag
     1381 gcctgcgcca agcttctgga cgtgcgctat gatgtggtgc cgatcaagga accggttgaa
     1441 ggatttctct cgacgctgaa gccgttgttc gagggcaaga attctgacgt caccgaagaa
     1501 aatatacagt cacgggcgcg cggcgtcatt ctcatggcga tctccaacaa gtttggctac
     1561 atggttctga ccacaggcaa caagtcggaa atg
//
LOCUS       DAORVN010000581         1593 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_91954_length_1593_cov_3.039662, whole genome shotgun sequence.
ACCESSION   DAORVN010000581 DAORVN010000000
VERSION     DAORVN010000581.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1593)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1593)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1593
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91954_length_1593_cov_3.039662"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..509)
                     /locus_tag="PKV23_10490"
     CDS             complement(<1..509)
                     /locus_tag="PKV23_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018447543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HQX85907.1"
                     /translation="MPRIKIDGVEIEIENGTTLLQACEQAGAEVPRFCFHERLSIAGN
                     CRMCLVEVKGTPKPQASCAMSVNDLRPGPNGEAPEVFTRTPQVKKAREGVMEFLLINH
                     PLDCPICDQGGECDLQDQAMAYGVDFSRYREPKRASEDLNLGPLVETKMTRCINCTRC
                     VRFTTEVAG"
     gene            complement(523..978)
                     /locus_tag="PKV23_10495"
     CDS             complement(523..978)
                     /locus_tag="PKV23_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009462957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3291 domain-containing protein"
                     /protein_id="HQX85908.1"
                     /translation="MHLAEVNIGKFKYPTTDPRMAEFMDNLDRINALAERSPGFVWRL
                     VGDNNNATDLRIGDDNAVNMSVWESPEALEHYVFKTVHVQFYKKRAAWFELMEKPHMV
                     FWWVEEGHRPDLQEALDRLEHYQKHGATEYAFGWAEVIDKERWHTQRCA"
     gene            complement(983..>1593)
                     /locus_tag="PKV23_10500"
     CDS             complement(983..>1593)
                     /locus_tag="PKV23_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009209803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HQX85909.1"
                     /translation="PNNTGTKLFCISGHVNKPCNVEEEMGIPLRELIEKHCGGVRGGW
                     DNLLAVIPGGASVPCVPAHQIMDSPMDFDSLRNLKSGLGTAAVIVMDKSTDMIRAIAR
                     LAQFYKHESCGQCTPCREGTGWMWRVLTRMADGRAEKREIDMLLEVTYQIEGHTICAL
                     GDAAAWPVQGLIRHFRHVIEERIDQYSHNADHEGSLARVAAE"
BASE COUNT          281 a          493 c          491 g          328 t
ORIGIN      
        1 atcccggcga cctcggtcgt gaaccgcacg cagcgggtgc agttgatgca gcgcgtcatc
       61 ttcgtctcga ccagcgggcc gaggttcagg tcctccgacg cccgcttagg ctcgcggtag
      121 cggctgaagt ccacgccata ggccatcgcc tggtcctgca gatcgcattc gccgccctgg
      181 tcgcagatcg ggcaatccag cggatggttg atgagcagga attccatgac gccttcgcgg
      241 gccttcttca cctgcggggt gcgggtgaaa acttccggcg cttcgccgtt ggggccgggg
      301 cgcaaatcat tcaccgacat ggcgcaggag gcctgcggtt tcggcgtgcc cttcacttcg
      361 acgaggcaca tgcggcagtt gccggcaatc gacaggcgct catggaaaca gaaacgcggc
      421 acttcggcgc cggcctgttc gcaggcctgc agcagcgtgg tgccgttctc gatctcgatc
      481 tcaacgccgt ctatttttat cctgggcatc ccgtctcgcc tctcaagcgc agcgctgggt
      541 gtgccagcgc tccttgtcaa tcacttcagc ccagccgaag gcatattcgg tcgcgccatg
      601 tttctggtaa tgctccaacc ggtccagcgc ttcctgcagg tccggccggt ggccctcttc
      661 aacccaccag aacaccatgt ggggcttttc catcagctca aaccaggcgg cgcgcttctt
      721 gtagaactgc acatggaccg tcttgaacac atagtgctcc agcgcttccg gcgattccca
      781 tacggacatg ttcacggcgt tgtcatcgcc aatgcggaga tcggtggcgt tgttgttgtc
      841 gcccacgagg cgccagacaa atcccggcga gcgctcggcc agggcattga tccgatcaag
      901 attgtccatg aactcggcca tgcgcggatc cgtggtcgga tacttgaact tgccaatatt
      961 gacttcagcg agatgcatga tcttactccg ccgccacgcg ggccaaggag ccctcatggt
     1021 ccgcattatg gctgtactgg tcgatgcgct cttcaatgac gtggcggaaa tggcggatga
     1081 ggccctgcac gggccatgcg gcggcatcac ccaacgcgca aatcgtgtgg ccttcgatct
     1141 ggtaggtcac ttcgagaagc atgtcgattt cgcgcttctc ggcgcggcca tcggccatgc
     1201 gggtgagcac gcgccacatc cagccggtgc cctcgcggca gggtgtgcac tggccgcagg
     1261 attcgtgctt gtagaattgc gcaagcctgg caatggcgcg gatcatgtcg gtggacttgt
     1321 ccatgacgat gacggcggcg gtgccgaggc ctgacttgag attgcgcagg gagtcaaagt
     1381 ccatgggcga atccataatc tggtgggcgg gcacgcaggg aaccgaagcg ccgccgggaa
     1441 tgactgccag caggttgtcc cagccgccgc gcacgccgcc gcaatgcttc tcgatcagtt
     1501 cgcgcagcgg aatgcccatt tcctcttcga cattgcaggg cttgttcaca tggccggaaa
     1561 tgcagaacag cttggtgccg gtattgttgg gcc
//
LOCUS       DAORVN010000582         1591 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_92116_length_1591_cov_4.706380, whole genome shotgun sequence.
ACCESSION   DAORVN010000582 DAORVN010000000
VERSION     DAORVN010000582.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1591
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92116_length_1591_cov_4.706380"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..971)
                     /locus_tag="PKV23_10505"
     CDS             complement(<1..971)
                     /locus_tag="PKV23_10505"
                     /inference="COORDINATES: protein motif:HMM:NF012350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HQX85910.1"
                     /translation="MAGAAGSHADKDHCCDSGTPPAPDQIAAAGSGTAFRIDTVDCAA
                     EESEIRRALAPVTAVRGLSFQLGARILTIDAPEPVIPEALVAIRKAGFDPQPVANDGP
                     AAGAIAPVSQGLWRLGFGLTLAVAAELLAFFAPETLLFKILGMALAAAAIGLAGLSTY
                     KKGLAALRRGQFNINALMAVAVTGAFLIGQWPEAAMVMALYAIAELIEGRALDRARNA
                     IKSLLDLAPDTAEVREADGDWKEIAATEVALETIVRVKPGARVPLDGVITAGTTAINQ
                     APVTGESIPVDKAPGDPVFAGTINDTGMIEMRVTAAANNTTLARIIDA"
     gene            complement(1122..>1591)
                     /locus_tag="PKV23_10510"
     CDS             complement(1122..>1591)
                     /locus_tag="PKV23_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009240708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HQX85911.1"
                     /translation="GESLNVKGAYLEVWADMLGSVGVIIGAVVIMTTGWTLVDPIIAV
                     LIGLWVLPRTWTLLREAGQVLMQGVPAGLDLDRVRDMLLAHPGVAKVHDLHVWALGSK
                     EPVLTAHIILTDETQPPDAVRQSLATALTAAFDIEHATLQVDASTVIEGPTHA"
BASE COUNT          301 a          508 c          463 g          319 t
ORIGIN      
        1 gcatcgatga tgcgcgccag ggttgtattg ttggcggccg cggtgacccg catctcgatc
       61 attccggtgt cgttgatggt cccggcgaac acggggtcgc ccggtgcctt gtcgacggga
      121 atgctttctc cggtaacagg tgcctgattg atcgcggtgg tgccagccgt gatcacgccg
      181 tccagcggta cccgcgcacc gggtttgacc cggacgatcg tttccagagc cacctcggtc
      241 gcggcgattt ccttccagtc gccgtcggcc tcccgcactt ctgcggtgtc gggggcgaga
      301 tcgagcaggc tcttgatggc gttgcgcgcg cgatccagtg cccgtccctc tatcagttcg
      361 gcaatcgcat aaagcgccat gaccattgcg gcttcgggcc attgccctat caggaacgcc
      421 ccggtcaccg ccacagccat caatgcattg atgttgaact gtccgcgacg aagcgcggcc
      481 aagcctttct tgtacgtcga caggccagcc aggccgatgg cggcggcggc cagcgccatg
      541 ccaagtatct tgaacagcaa tgtttccggc gcgaagaagg ccagcaattc cgccgctacg
      601 gcaagcgtca agccaaatcc cagacgccac agtccctggg agacaggcgc aattgcgccc
      661 gcggctgggc catcgttcgc aaccggttgg gggtcaaagc ctgctttccg aatcgccacc
      721 agcgcctctg gtatcaccgg ctccggagca tcgatggtga ggatccgtgc gcccaactgg
      781 aaactgagcc cgcgcaccgc ggtgactggc gccagagcgc ggcggatttc gctttcctca
      841 gcggcgcaat ccacggtgtc tatgcggaaa gccgttccac tgccagccgc cgcgatctga
      901 tctggtgcgg ggggagtacc agagtcacaa caatgatctt tgtcagcgtg cgaaccagcg
      961 gcgcctgcca cgtcaacttc ttttgtattc acaaacttct ccggtaacta ttcttctaga
     1021 cgttgatgca ctgaaggacc atgcgaaatc aatgtaggcc gtggcccaat cgaaaatcag
     1081 cgatcttgat gtttgcttgc tgcaacgccg aagcgagtcc tctacgcatg cgttggacct
     1141 tcgatcaccg tggaggcgtc tacctgcaag gtcgcgtgtt cgatatcaaa tgctgctgtc
     1201 aacgccgtcg ccaacgactg ccgcaccgca tccggcggtt gcgtttcgtc ggtaaggatg
     1261 atgtgcgcag tcaatacggg ttcctttgac ccgagcgccc agacgtgcag gtcgtgaacc
     1321 tttgcgaccc cgggatgcgc caacaacatg tcgcgaactc tgtccaggtc aagacctgca
     1381 ggtacgcctt gcatcaacac ctggcccgcc tcgcgcaaca gcgtccatgt gcgcggcaga
     1441 acccacaggc cgatcagcac ggcaatgatt ggatctacca acgtccagcc ggtggtcatg
     1501 attaccaccg cgccgatgat gacgcctacc gacccgagca tgtcggccca gacttcaaga
     1561 tacgcgcctt tgacgttgag gctttcgccg c
//
LOCUS       DAORVN010000583         1590 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_92238_length_1590_cov_3.114007, whole genome shotgun sequence.
ACCESSION   DAORVN010000583 DAORVN010000000
VERSION     DAORVN010000583.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92238_length_1590_cov_3.114007"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..592)
                     /locus_tag="PKV23_10515"
     CDS             complement(<1..592)
                     /locus_tag="PKV23_10515"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175479.1"
                     /note="Catalyzes first step of the de novo purine
                     nucleotide biosynthetic pathway; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HQX85912.1"
                     /translation="MTEDFDINGDRLREECGVFGVYGHPDAAALTALGLHALQHRGQE
                     AAGIVTFDGERFNAERRMGLVGDHFSSEKVINRLQGRSSLGHVRYSTTGETILRNVQP
                     LFAELSAGGLAVAHNGNITNGLTLRRELISQGAICQSTSDTEVILHLVSRSKKSRIID
                     RYIDALRSLEGAYALVALTNKKLIGARDPLGIRPLIL"
     gene            complement(589..1254)
                     /locus_tag="PKV23_10520"
     CDS             complement(589..1254)
                     /locus_tag="PKV23_10520"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HQX85913.1"
                     /translation="MPFQLLDLILFGIMLISGLLALMRGFTREVLSLVAWGAAAFAAY
                     FAIKQQALLDLALPYVDKPILAQIAVGAIAFIITLIVVSLISVKISDSVVDSAAGAFD
                     RTLGFIYGLARGFVLVAIAYLFYGWLLPFDKQEDWVRNASSLPAIKSVGETLLTFMPP
                     DIAETLSNTALMKNPDAPAGTVTQPAPAPGYQSGETQGLDNLIEGTGNTTQQPEFGQS
                     TGQ"
     gene            complement(1311..>1590)
                     /locus_tag="PKV23_10525"
     CDS             complement(1311..>1590)
                     /locus_tag="PKV23_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009209370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HQX85914.1"
                     /translation="AVAAALLSSLTGTVIPHGTAVFGEVALSGAIRPVGQTEARLKEA
                     QKLGLREAVIAAATEQASLKGLKVKSIETISDLVAWTAAQDKGLRRIV"
BASE COUNT          328 a          408 c          555 g          299 t
ORIGIN      
        1 ccaggatgag cgggcgaatg ccgaggggat cacgcgcgcc gatgagtttc ttgttggtca
       61 gcgccacgag ggcgtaggcg ccttcgaggc tgcgcagcgc gtcgatgtag cggtcaatga
      121 tgcggctctt cttcgagcgc gagacgagat gcaggatcac ttccgtatcg gaggtggact
      181 ggcagatggc gccctggctg atgagttcgc ggcggagcgt caggccgttg gtgatgttgc
      241 cgttgtgggc gacggcgagg ccgccggcgg aaagttcggc gaagaggggc tggacgttgc
      301 gcaggatggt ttcgcccgtg gtggaatagc ggacatggcc gagactggaa cgaccctgca
      361 ggcggttgat gaccttctcg ctggaaaaat gatcaccgac caggcccatg cggcgttcgg
      421 cgttaaagcg ttcgccatca aaagtgacaa tgccggcggc ttcctggccg cgatgctgaa
      481 gggcgtggag gcccagagcc gtcagcgctg ccgcatcagg atggccataa acgccgaaga
      541 caccgcattc ttcccgtaag cggtcaccgt tgatgtcgaa gtcttctgtc attggccggt
      601 gctttgtccg aattcgggtt gctgagtggt attgccggtg ccttcaatga gattgtccag
      661 tccctgggtt tcgccggact gatagccagg agcaggggct ggctgggtga ccgtgcctgc
      721 cggggcatcg ggatttttca tcagtgcggt gttggacagg gtttcggcaa tgtccggcgg
      781 catgaaggtg agcagggttt cgccgaccga cttgatggcc ggaagggaag aggcattgcg
      841 cacccaatct tcctgcttgt cgaagggcag gagccagcca tagaaaagat aggcgatggc
      901 cacgagaacg aaaccacggg caaggccata gataaaacca agggtgcggt caaaagcgcc
      961 ggcggcggag tcgacaaccg aatcggaaat cttcacgctg atcagcgaca cgacgatgag
     1021 tgtgatgatg aaggcgatgg cgcctacggc gatctgcgcg aggatgggct tgtcgacata
     1081 gggcaaggcg agatcaagca gggcctgctg cttgatggcg aagtatgcgg cgaaggcggc
     1141 ggcgccccag gcgaccagtg aaaggacctc gcgggtgaaa ccgcgcatca gggcaaggag
     1201 cccggagatc agcatgatgc cgaaaaggat gaggtcaagc agctggaagg gcataaaaaa
     1261 ttcccggttg gtcggatgag ccaccttata ggcagctttt gccaactgat ctagacgatt
     1321 cgtcgcaaac ctttgtcctg tgcagcagtc cacgccacga gatcagaaat ggtttcaatt
     1381 gacttcactt tcaagccctt gaggctcgcc tgctcggtgg ctgcggcaat gacggcttcg
     1441 cgcaagccca gcttttgcgc ttccttgagg cgggcttcgg tttggccgac agggcgaatt
     1501 gcgcctgaaa gggccacttc accgaagacg gcggtgccgt ggggaatcac ggttccggtg
     1561 agcgaggaga gcagcgctgc ggccacggcg
//
LOCUS       DAORVN010000584         1589 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_92344_length_1589_cov_3.561930, whole genome shotgun sequence.
ACCESSION   DAORVN010000584 DAORVN010000000
VERSION     DAORVN010000584.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1589
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92344_length_1589_cov_3.561930"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /gene="atpH"
                     /locus_tag="PKV23_10530"
     CDS             <1..251
                     /gene="atpH"
                     /locus_tag="PKV23_10530"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HQX85915.1"
                     /translation="ALVAESKGEVAAEVTSAEKLSEKHIKDIKAALKSSMGRDVQLTA
                     KIDSSLLGGLIVKVGSKMMDNSLKTKLQNLKIAMKGTG"
     gene            251..>1589
                     /gene="atpA"
                     /locus_tag="PKV23_10535"
     CDS             251..>1589
                     /gene="atpA"
                     /locus_tag="PKV23_10535"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015917383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HQX85916.1"
                     /translation="MDIRAAEISTILKKQIKDFGNEAEVSEIGQVLSVGDGIARVYGL
                     DNVQAGEMVEFPGGIRGMALNLETDNVGVVIFGNDRDIKEGDTVKRTGAIVEVPVGKE
                     LLGRVVDALGNPIDGKGPIKSKLRSRVDVKAPGIIPRKSVHEPMSTGLKAVDALIPIG
                     RGQRELIIGDRQTGKTAIILDAILNQKPIHQNGPEKEKLYCVYVACGQKRSTVAQFVK
                     ILEDAGALEYSIIVAATASESAPMQYLAPFTGCTMGEYFRDNGMHALIAYDDLSKQAV
                     AYRQMSLLLRRPPGREAYPGDVFYLHSRLLERAAKLSDAKGAGSLTALPVIETQANDV
                     SAYIPTNVISITDGQIFLETDLFYQGIRPAVNVGLSVSRVGSAAQIKAMKQVAGKIKG
                     ELAQYREMAAFAQFGSDLDASTQRLLNRGARLTELLKQPQFSPLKTEEQVVAIY"
BASE COUNT          349 a          461 c          479 g          300 t
ORIGIN      
        1 cggctctggt tgccgagtca aaaggcgaag tcgcggcaga agtgacttcg gcggaaaagc
       61 tttccgaaaa gcatatcaag gacatcaagg cggcgctcaa atcctccatg ggacgcgacg
      121 tgcagctcac tgccaaaata gattcctccc ttctcggcgg cctcatcgtc aaggtgggct
      181 ccaagatgat ggacaattca ctcaagacaa aattgcaaaa ccttaagatc gctatgaagg
      241 ggaccggctg atggatattc gcgccgctga gatttcgaca attcttaaaa agcagatcaa
      301 ggacttcggc aatgaagccg aagtatccga aatcggccag gtgctttcgg tgggcgacgg
      361 cattgcccgc gtctacggcc tcgacaatgt gcaggccggc gaaatggtgg aattcccggg
      421 cggcatccgc ggcatggcgc tgaacctcga aaccgacaat gtcggcgtcg ttattttcgg
      481 caacgaccgc gacatcaagg aaggcgacac ggtcaagcgc accggcgcca tcgtggaagt
      541 gccggtgggc aaggaactgc tgggccgggt ggttgacgcg ctgggaaatc ccattgacgg
      601 caagggcccg atcaagtcga agctgcggtc gcgcgttgac gtgaaggcgc cgggcatcat
      661 tccgcgcaag tcggtgcatg agccgatgtc gacgggcctg aaggcggttg acgcacttat
      721 cccgatcggc cgcggccagc gcgaattgat cattggtgac cgccagaccg gcaaaacggc
      781 gatcattctc gatgccatcc tgaaccagaa gcctattcac cagaacgggc cggaaaaaga
      841 aaaactctat tgcgtatacg tggcctgcgg acagaagcgt tcgaccgtgg cccaattcgt
      901 gaagatcctg gaagatgcag gcgcgcttga gtattcgatc atcgtggcgg ccactgcttc
      961 ggaatcggcg ccaatgcagt atctggcccc gttcacgggc tgcaccatgg gtgaatattt
     1021 ccgcgacaac ggcatgcatg cactcatcgc ctatgacgat ctttccaagc aggccgtggc
     1081 ttatcgccag atgtccctgc tgctccgccg cccgccgggc cgcgaagcct atccgggcga
     1141 cgtgttctat ctgcattcgc gcctgctgga gcgtgcggcg aagctttcgg acgccaaggg
     1201 cgccggttcg ctcacggcgt tgccggttat tgaaacccag gccaacgacg tttcggctta
     1261 catcccgacc aacgtaattt cgatcaccga tggccagatc ttccttgaaa cggatctgtt
     1321 ctaccagggc atccgcccgg cggtgaacgt cggcttgtcc gtgtcgcgcg taggctcggc
     1381 agcacagatc aaggcgatga aacaggttgc cggcaagatc aagggcgagc tggcgcagta
     1441 ccgcgaaatg gcggcgtttg cccagttcgg ctcggatctc gacgcctcaa cgcagcggct
     1501 tctcaaccgc ggcgcgcgcc tcacggaact tctcaagcag ccgcagttct cgccgctcaa
     1561 gacagaagag caggttgtgg cgatctatg
//
LOCUS       DAORVN010000585         1587 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_92581_length_1587_cov_2.281332, whole genome shotgun sequence.
ACCESSION   DAORVN010000585 DAORVN010000000
VERSION     DAORVN010000585.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1587
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92581_length_1587_cov_2.281332"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="PKV23_10540"
     CDS             complement(<1..306)
                     /locus_tag="PKV23_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003565398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlgM"
                     /protein_id="HQX85917.1"
                     /translation="MRLENEKPSENVEDRRGQGGGMGRGFGFPRGGSGRGINIPMGGG
                     RGGFSISTLVMLVIVYFAVKFIFGIDLLQVLNGGGIQVPGGGTSTEMTIPSGNTDVAG
                     "
     gene            complement(327..1298)
                     /locus_tag="PKV23_10545"
     CDS             complement(327..1298)
                     /locus_tag="PKV23_10545"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:NF005089.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HQX85918.1"
                     /translation="MTSICVFGAGAIGGYIGARLAHKGEAEVSLVARGPHLAAMEANG
                     LILKQGGETITVHPKVSSDPKTLGPQDYVIVGVKAHALTGVLDQLQPLLGKNTALLFA
                     QNGLPWWYFYKHGGPYEGRRIESVDPGGKIWERLGPERALGSVVWQAAEMEAPGIIAH
                     AYGDRLPIAEPSGEKTPRAMLLSKLLMSAGLKSPVRGNLRDEIWLKLWGNLSFNPVSV
                     LTQGTLLQLATDPGTRKVIRDMMQEARLVGEALGVKFSVDVDERIAMAEKVGNHRTSM
                     LQDVEAGRPTELDSLLGVVIELAQMVGIATPSLNLVYDLTRFSSKTR"
     gene            complement(1295..>1587)
                     /locus_tag="PKV23_10550"
     CDS             complement(1295..>1587)
                     /locus_tag="PKV23_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015950867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-dependent synthetase"
                     /protein_id="HQX85919.1"
                     /translation="GEKISPAEIDEALIDHPAVQQALAFAVPHDKLGEDVAAVVVLKE
                     GVTATERELRDFVNEKLAAFKVPNRIYFLSEIPKGATGKLQRIGLAQKLGLV"
BASE COUNT          299 a          486 c          468 g          334 t
ORIGIN      
        1 gccagccaca tcggtgttgc cgctagggat ggtcatctcg gtgctggtgc caccgcccgg
       61 cacctgtatg ccgccgccat tgagaacctg aagcaaatca attccaaaga taaacttgac
      121 ggcaaaatac acgataacca gcatgacaag cgttgaaatg ctgaagccgc cgcgtccgcc
      181 gcccatggga atgttgattc cccggccgct tccgccacgg gggaagccga agccgcggcc
      241 catgccacca ccctgccctc ggcggtcttc aacattttcg ctgggctttt cattctcgag
      301 gcgcatgggt ttttctccgg tgggttctat ctggttttgc tgctgaacct ggtgaggtca
      361 tataccaagt tgaggctggg tgtagcaatt ccaaccatct gtgccaattc tatgacaacg
      421 ccgagcagtg aatcgagttc cgtgggccgt ccggcctcga cgtcctgcaa catggaggtg
      481 cggtggttgc cgactttttc cgccatggcg atgcgttcat cgacatccac ggagaatttg
      541 acgcccagcg cttcgccgac aaggcgggcc tcctgcatca tatcacggat gaccttccgt
      601 gtacccggat cggtggcaag ctggaggagc gttccctggg tcaggacact gaccggattg
      661 aaactcaggt tgccccagag cttcagccag atctcatcac gcaggttgcc gcgcaccggg
      721 cttttcaatc cggcgctcat cagcagcttg ctgagcagca tggcgcgcgg ggttttttct
      781 cccgaaggtt cggcaatcgg caggcggtca ccataggcgt gggcgataat gcccggcgct
      841 tccatttcgg cggcctgcca gacaacgctg ccaagggcgc gctctggccc cagccgctcc
      901 cagatctttc caccgggatc aacgctttca atgcgccgcc cttcataggg gccgccgtgc
      961 ttgtagaaat accaccaggg caagccattc tgggcaaaga gaagggcggt gttcttgccc
     1021 agcaagggct ggagctggtc cagcacgccg gtgagcgcat gggccttgac cccgacaatg
     1081 acatagtcct gcggccccag ggttttcgga tcagatgata ctttgggatg cacggttatg
     1141 gtttcgccgc cctgtttcag gatgaggccg ttggcttcca tggcggcaag atgggggccc
     1201 cgggcgacaa gcgaaacttc ggcttcgccc ttgtgcgcca gccttgcgcc gatatagccg
     1261 ccaatggcgc cagccccaaa gacgcagata cttgtcatac caggcccagt ttctgcgcca
     1321 ggccgatgcg ctgcagtttg cccgtggcgc ccttgggaat ttcgctcaga aaataaatcc
     1381 ggttgggcac tttgaaggct gcaagctttt cattcacgaa gtcgcgcaac tcccgttcgg
     1441 ttgccgtgac gccctccttc aggacgacga cggcggccac atcctcgccg agcttgtcat
     1501 ggggaaccgc aaaggccagg gcctgctgga cggcaggatg atcgatcagc gcctcgtcga
     1561 tttcggcggg agagatcttt tcgccgc
//
LOCUS       DAORVN010000586         1585 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_92776_length_1585_cov_2.435294, whole genome shotgun sequence.
ACCESSION   DAORVN010000586 DAORVN010000000
VERSION     DAORVN010000586.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1585
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92776_length_1585_cov_2.435294"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..585)
                     /locus_tag="PKV23_10555"
     CDS             complement(<1..585)
                     /locus_tag="PKV23_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015343352.1"
                     /note="has catalase and peroxidase activities; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase-peroxidase"
                     /protein_id="HQX85920.1"
                     /translation="MDKKTDESVGQCPVVHRTAARANRDWWPNQLSLQVLHQHSSLSN
                     PMGEAFNYADEFKKLDIEALKKDLTALMTDSQEWWPADFGHYGPLFVRMAWHSAGTYR
                     IGDGRGGAGAGQQRFAPLNSWPDNVNLDKARRLLWPIKQKYGNKISWADLMILTGNVA
                     LESMGFKTFGFAGGREDVWEPDHDVYWGTETTWLG"
     gene            815..1375
                     /locus_tag="PKV23_10560"
     CDS             815..1375
                     /locus_tag="PKV23_10560"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HQX85921.1"
                     /translation="MKSLTILPFADTPNLIVREIESYDVRAFSAFMMQPRYQRYTAMR
                     LRSDAEVAAFVLRSVARQGDDRRNVFHLAAEERSSAEAIGDGFLIMQPDRTVELGWGV
                     HPAMWSCGFGTEIGAALLGLSFERLKAKSVWCKVMRANTASAKLASRIGMAHEKRVAG
                     YAVGDGRYEDVDIYAMTAAEYFDLPY"
     gene            1384..>1585
                     /locus_tag="PKV23_10565"
     CDS             1384..>1585
                     /locus_tag="PKV23_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HQX85922.1"
                     /translation="MAAFPPLCDFGWKAPAFSLPGVDGKRHTLESLRGPKGTLVAFIC
                     NHCPYVKAVIGRLVSEAAELRRL"
BASE COUNT          296 a          499 c          447 g          343 t
ORIGIN      
        1 ccccaaccag gtggtttccg tgccccaata cacatcatgg tccggttccc agacatcctc
       61 gcgtccgccg gcaaaaccga aggtcttgaa ccccatcgac tcaagggcaa cgttgcccgt
      121 gaggatcatc aggtcggccc acgaaatctt gttgccgtat ttctgcttga tcggccacag
      181 cagccgccgc gccttgtcca ggttcacgtt gtccggccag ctgttgagcg gcgcgaaacg
      241 ttgctgcccc gctccggcac cgccgcggcc atcgccgatg cggtaagtgc ccgcgctatg
      301 ccacgccatc cggacgaaca gcggcccgta gtggccaaag tccgccggcc accactcctg
      361 gctgtccgtc atcagtgcgg taagatcctt cttgagcgcc tcaatatcga gtttcttgaa
      421 ttcatccgca tagttgaaag cctcgcccat gggattggaa aggctggaat gctggtgcag
      481 aacctgaagg cttagctggt ttggccacca gtcccggttg gcccgggcgg cagtcctgtg
      541 cacgacgggg cactggccaa cgctctcatc agtcttttta tccatggttc tctccaatcg
      601 tgttctgtaa attgttttga tgttcgggag gctgttggca tctccctgtt cgcgcattga
      661 ttttgatcaa ttctagtctg gaaactgccc gcaattcaat cgccaagagc aagctgcgac
      721 accacgggcc cctcctggac actggttcca atcgaaacaa agtcaggcaa attgaaggct
      781 tcacaggcag gaattttgct tatagtgttt ggccatgaag tcgctgacca tacttccgtt
      841 cgccgacacg ccaaatctca ttgtgcgcga gatcgagtcg tatgacgtca gggctttttc
      901 cgcattcatg atgcagcccc gctaccagcg ctatacggcc atgcgcctgc gcagcgacgc
      961 ggaggtggct gcctttgtcc tgcgctcggt tgccaggcag ggcgacgacc gccgcaatgt
     1021 cttccatctc gccgccgagg agcgttcctc cgccgaggcg attggcgatg gcttccttat
     1081 tatgcagcct gatcgcaccg tcgaactggg atggggggtt catcccgcca tgtggagctg
     1141 cggctttggc accgaaatcg gcgctgccct ccttgggctc agcttcgaac gcctcaaggc
     1201 gaagagcgtc tggtgcaagg tcatgcgggc aaacaccgcc tcggccaaac tcgcaagccg
     1261 catcggcatg gcccacgaaa agcgcgtggc gggctacgcg gtgggcgacg ggcgctacga
     1321 ggatgtcgat atctacgcca tgaccgcagc agaatacttc gatctcccgt actgagcagc
     1381 accatggcag cctttccgcc tctttgtgat ttcggttgga aagcacctgc cttcagcctg
     1441 cctggcgttg acggcaagcg ccacacgctg gaaagccttc gcggccccaa gggcacgctc
     1501 gtagccttca tctgcaacca ttgcccctat gtgaaagccg tcattggccg cctggtgagc
     1561 gaggctgccg agcttcggcg cctag
//
LOCUS       DAORVN010000587         1583 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_92988_length_1583_cov_2.649215, whole genome shotgun sequence.
ACCESSION   DAORVN010000587 DAORVN010000000
VERSION     DAORVN010000587.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1583
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92988_length_1583_cov_2.649215"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..888
                     /locus_tag="PKV23_10570"
     CDS             <1..888
                     /locus_tag="PKV23_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006613079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HQX85923.1"
                     /translation="KVAIREPATREGWAKLMKRLGVKGIHIAERDTQRSKNPKPLDVF
                     INTWSVDGFISEGLQPAELGWGTHEKKLPYDGKKHKKGTGAAIYLTRAGADTRVRSWV
                     PTAGAHFGLLVTHNEAISISDYFTVRVGKRVTYRPTCHYAYHPSNDAVLSVLEMFGAG
                     GRRQSASHILEEKEIVDGGDELGVFLYGHKKNAYWYGSQLSIEEARKLAPYQNATGMQ
                     VTSAVLAGLVWAIENPKAGIVETEEMDFRRCLEIQTPYLGTLRGVYTDWTPLQGRGEL
                     FEEKLDRRDPWQFSNIIVR"
     gene            900..>1583
                     /locus_tag="PKV23_10575"
     CDS             900..>1583
                     /locus_tag="PKV23_10575"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HQX85924.1"
                     /translation="MNLVRTLTSLAAICMLAVTAYATPQHGIAMHGEPALPADFEHLP
                     YVNPDAPQGGALRMAITGSFDSLSPFIVKGTPVMGVRTYVFESLMGRNWDEPFSLYGL
                     LAESIDVSDDRQTFTFKIRPEAKFSDGKPVTAADVVFTVETLRDKGRPNFKNSYSKIS
                     KIETPDDHTVIFHQEVGDRELPLIVGVMPILPKHAWEGKTFDESSLEPMVGSGPYVIA
                     EVRAGESITY"
BASE COUNT          341 a          441 c          495 g          306 t
ORIGIN      
        1 aaggtcgcaa tcagggaacc agccacgcgc gagggttggg caaagctgat gaagcggctg
       61 ggcgtgaagg gcattcatat tgccgagcgc gatacgcagc gctccaagaa cccgaagccg
      121 ctggatgtat tcatcaacac ctggtcggtg gacggattca tttccgaggg cctgcagccg
      181 gccgagcttg gctggggcac gcatgaaaag aagctgccat acgacggcaa aaagcacaaa
      241 aagggcacgg gggccgcaat ctatctcacc cgggccgggg ccgatacacg ggtgcggagc
      301 tgggttccca cggcgggggc gcatttcggg ctgctggtta cccataacga ggctatttcc
      361 atttccgatt acttcacggt gcgcgtggga aagcgcgtca cctaccggcc cacctgccac
      421 tatgcctatc acccctccaa tgacgcggtg ctttccgtgc ttgaaatgtt tggcgccggg
      481 gggcggcggc aatcggcttc gcatatcctg gaagagaagg agatcgtgga cggcggagat
      541 gaacttgggg ttttcctcta tggccacaag aagaatgcct actggtatgg ctcgcagctt
      601 tccatcgaag aggcgcggaa gctggccccc taccagaatg ccacgggcat gcaggtaacc
      661 tcggcagtac tggcgggact ggtctgggcc attgaaaacc ccaaggccgg gatcgtcgag
      721 acggaagaga tggatttccg ccgctgcctt gaaattcaaa cgccttatct tgggacactg
      781 cgcggggttt acacggactg gacgccgctg cagggccggg gcgaactgtt tgaagagaaa
      841 ctggaccggc gcgacccctg gcaattttcg aacattattg tgcgctagcg gatggcgcca
      901 tgaatcttgt tcggacgctc acgtctcttg ctgccatctg catgcttgca gttacggcat
      961 atgcaacgcc acagcacggc attgcgatgc atggcgagcc ggcgcttccg gcggatttcg
     1021 agcacctgcc ttacgtgaat ccggatgcgc cgcagggcgg cgccctgcgc atggccatta
     1081 ccggatcatt tgacagcctt tcaccgttca tcgtgaaggg cacgccggtc atgggggttc
     1141 gcacctatgt gttcgaaagc ctgatggggc ggaactggga cgaacccttt tccctctacg
     1201 ggctgctggc ggaaagcata gatgtcagcg acgaccggca gacattcacg ttcaagatca
     1261 ggcctgaggc caaattttcc gatggcaagc cggtgacggc ggcggacgtg gtgttcacgg
     1321 tggaaaccct gcgcgacaag ggccgaccga atttcaagaa ctcctattcg aagatcagca
     1381 agatcgaaac tccggacgac catacggtca tcttccatca ggaggtcggc gacagggagc
     1441 ttccactgat cgtcggcgtg atgcccatcc ttccgaaaca tgcctgggaa gggaaaacct
     1501 ttgacgagag ttcgcttgag ccgatggtcg gttccggtcc ctatgtgatc gccgaagtga
     1561 gagcgggaga aagcatcacc tac
//
LOCUS       DAORVN010000588         1578 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_93512_length_1578_cov_2.469468, whole genome shotgun sequence.
ACCESSION   DAORVN010000588 DAORVN010000000
VERSION     DAORVN010000588.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93512_length_1578_cov_2.469468"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            319..1059
                     /locus_tag="PKV23_10580"
     CDS             319..1059
                     /locus_tag="PKV23_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011159890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HQX85925.1"
                     /translation="MSKIVQLKATQRGRAGKGASRAVRREGLVPGVVYGDKQAAQMVS
                     LNYRELEPHIQTGRFLSTLVDLEVEGKTIRAIPRDVQFEPIRDFIVHVDFLRLGKASR
                     IAVDIPVHFKGQEASPGIKLGGSLNIVSHTLSLYCSADFIPDEITVDVAGLQIGDSIH
                     MSDIKLPEGVTAVSRDDVTLCTIAAQAKEEVVEEVAAPVDVPATAQKAPAEGAAAAAP
                     AAGAKGAAPAAAPAKGAAPAAAPAAKKK"
     gene            1086..>1578
                     /gene="pth"
                     /locus_tag="PKV23_10585"
     CDS             1086..>1578
                     /gene="pth"
                     /locus_tag="PKV23_10585"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014329128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HQX85926.1"
                     /translation="MFLLVGLGNPGTKYAGNRHNIGFMAVDEIVRRHGFSPWRKKFHG
                     EISEGLLALQKTLVLKPLTFMNESGQAVGEAIRFLNMEPEQVIVIYDEIDLMAGKVRV
                     KLGGGAAGHNGIRSITAHIGPHYTRVRLGVGHPGDKTLVHPHVLSDFAKADILSLAAA
                     LERG"
BASE COUNT          311 a          478 c          484 g          305 t
ORIGIN      
        1 gcatgcggga ggaaaagtca gaaaatccga gtttcaaaac gccgctccgc cccggccgtc
       61 cgccgggctc ccgccggagg gacgttcatc aggttgatga actgctcagc gagtgccagt
      121 ggctggcctg ggaggcgatg aagcccgata caagctgaac aggcggaaaa atgccctgat
      181 tcctggttaa aacctcctct ttccagagcg gggttgcaac gccttgcccc ctcatctata
      241 ccccgccgat cacggcacag gcagcggaca cccctggagg caggcccggg ccgtggttca
      301 ttcattcagg agttaaaaat gtccaagatc gttcagctta aggccacgca gcgtggccgg
      361 gcaggcaagg gggcctcccg cgcagtacgt cgcgaaggtc tcgttcccgg tgtcgtttat
      421 ggcgacaagc aggcagccca aatggtttcg ctcaattacc gcgagctcga gccccatatc
      481 cagaccggcc gcttcctgtc gacgcttgtc gacctcgaag tggaaggcaa gaccatccga
      541 gccatcccgc gcgatgtgca gttcgagccc atccgcgatt ttatcgtgca tgtggatttc
      601 ctgcggcttg gcaaggcctc gcgcattgcc gttgacatcc cggtgcattt caagggccag
      661 gaagcctcgc cgggcatcaa gctcggcggc tcgctcaaca tcgtgtcgca tacgctcagc
      721 ctttactgct cggcggattt cattcccgat gaaatcaccg ttgatgtggc cggcctgcag
      781 atcggcgact cgatccatat gtccgacatc aagctgccgg aaggcgtgac cgcggtttcg
      841 cgcgatgacg tgacgctgtg cacgatcgcc gcccaggcca aggaagaagt ggtcgaggaa
      901 gtggcggctc cggttgacgt tccagccacg gcccagaaag ctcctgcgga aggcgcggct
      961 gctgccgctc cggcagcggg tgccaagggt gcggctcctg ccgcagcgcc tgccaagggt
     1021 gcggctccag cggctgctcc ggctgcaaag aagaagtaat catcttcgtc tgacaggaga
     1081 cgggcatgtt tcttctcgtg ggtctcggca atccgggaac caaatatgcc ggaaacaggc
     1141 acaatatcgg cttcatggcg gtggacgaaa tcgtccgccg ccatggcttt tcgccctggc
     1201 gcaaaaagtt ccacggcgag atttccgaag gcctcctggc gctgcagaaa acgctggtcc
     1261 tcaagcctct gaccttcatg aatgaatcgg ggcaggcagt aggcgaagcc atccgttttc
     1321 tcaacatgga gccggaacag gtcatcgtca tctatgacga gattgatctg atggcgggga
     1381 aagtccgggt gaagcttggc ggtggggcgg cgggccacaa cggcatccgc tcgatcaccg
     1441 cccatatcgg cccgcattat acgcgggtgc gtctgggggt tggccatccg ggtgacaaga
     1501 ctctggttca tccccatgtc ctcagtgatt tcgccaaggc cgacattctg tcgctggctg
     1561 cggcccttga acgcggat
//
LOCUS       DAORVN010000589         1577 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_93617_length_1577_cov_2.580815, whole genome shotgun sequence.
ACCESSION   DAORVN010000589 DAORVN010000000
VERSION     DAORVN010000589.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93617_length_1577_cov_2.580815"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1059)
                     /gene="recA"
                     /locus_tag="PKV23_10590"
     CDS             complement(<1..1059)
                     /gene="recA"
                     /locus_tag="PKV23_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009468579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HQX85927.1"
                     /translation="MGQTNLRVVENSSMDKNKALDAALSQIERAFGKGSIMKLGSNPV
                     VEIEAISTGSLGLDIALGIGGLPRGRVIEIYGPESSGKTTLALQTIAESQKRGGVCAF
                     IDAEHAMDPGYAKKLGVDVQELLISQPDTGEQALEIADTLVRSGAIEILVIDSVAALT
                     PKAELEGEMGDSLPGLQARLMSQALRKLTASISKSNCMVIFINQIRMKIGVMFGSPET
                     TTGGNALKFYASVRLDIRRIGAIKDREEVVGNQTRVKVVKNKVAPPFKLVEFDIMYGE
                     GISKVGELVDLGVTAGVVEKSGSWYSYNGERIGQGRDKAKEFLKLNPDMANAIEAAIR
                     ANAGLIAAKITVAPTAEES"
     gene            complement(1218..>1577)
                     /locus_tag="PKV23_10595"
     CDS             complement(1218..>1577)
                     /locus_tag="PKV23_10595"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HQX85928.1"
                     /translation="IEAAGGISAADYLAIVKRREAMIGRFNSAIHGFSAVALPTVMII
                     PPPISALGTDQEYFRYNSMSLRNTYVGSFLNCCALSLPVNEPGAAPVGLMLMGVWGQD
                     QGLFSIGKAIETLLQSG"
BASE COUNT          341 a          482 c          424 g          330 t
ORIGIN      
        1 ggactcctcg gccgtcggcg ccaccgtgat cttggcggca atcagcccgg cattggcgcg
       61 gatggccgcc tctatggcgt tggccatgtc cggattgagt ttgaggaatt ccttggcctt
      121 gtcccggccc tgcccgatgc gctcgccgtt ataggagtac caggaaccgg acttctcaac
      181 gaccccggcg gtaaccccca ggtcaacaag ttcgccaact ttggaaatac cctcgccata
      241 catgatatcg aactcaacca gcttgaaggg cggcgccacc ttgttcttga ccaccttcac
      301 ccgggtctgg ttgccaacca cctcttcgcg gtccttgatg gcgccaatcc ggcggatatc
      361 gaggcgcacc gaggcataga atttaagggc attgccgccg gtcgtggttt ccgggctgcc
      421 gaacatcacg ccgatcttca tgcggatctg gttgatgaaa atgaccatgc agtttgattt
      481 cgagatcgac gccgtcagct tgcgcagcgc ctggctcatc aggcgggcct gcaggccggg
      541 cagtgaatcg cccatttcgc cttcaagctc ggccttgggc gtgagtgctg ccaccgaatc
      601 gatcaccagg atctcaatgg cgccggagcg caccagcgta tcggcgattt caagcgcctg
      661 ctcgcccgtg tcgggctgcg aaatgagaag ctcctggaca tcgaccccga gcttcttggc
      721 atagcccgga tccatggcgt gttcggcgtc aataaaggcg cagacgccgc cgcgcttttg
      781 cgattccgca atggtctgca gcgccagcgt ggtcttgcct gaggattcag ggccataaat
      841 ttcaatcacc cgcccgcgcg gcaggccgcc gatccccagg gcaatgtcga gaccgagaga
      901 acccgtcgaa atcgcttcaa tttcaacaac cggattggac cccagcttca tgattgaacc
      961 cttgccgaaa gcccgttcaa tctggcttag cgccgcgtcc agcgccttgt tcttgtccat
     1021 tgaagaattc tcaactaccc gaagattcgt ctgccccatc gcccgtactc cttaagtcag
     1081 atgcctcgct cggtgcaatc acttcctttg taccagcgat gttgacgtta tgttctaatt
     1141 ttgttccaaa ggcaagagga atttgtgcgg gaaacaaaaa attgcagcgg gccgcccaaa
     1201 tccttggaat tacacgatca gcccgattga agcagggttt cgattgcctt cccaatagaa
     1261 aagagaccct gatcctgacc ccacacaccc atgagcatta ggccaaccgg cgccgccccg
     1321 ggctcattca ccggaagaga cagggcacag caattcagga aacttcccac atacgtattg
     1381 cgcaggctca tggaattata gcggaaatat tcctgatcag ttcccaacgc cgaaatgggc
     1441 ggcggaataa tcataacggt agggagggca accgcagaaa aaccatggat ggcggaattg
     1501 aagcgcccga tcatcgcctc ccggcgtttc acgatggcga ggtaatcagc ggcgctgatg
     1561 ccgccagccg cttcaat
//
LOCUS       DAORVN010000590         1574 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_93899_length_1574_cov_3.373930, whole genome shotgun sequence.
ACCESSION   DAORVN010000590 DAORVN010000000
VERSION     DAORVN010000590.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93899_length_1574_cov_3.373930"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            130..1140
                     /locus_tag="PKV23_10600"
     CDS             130..1140
                     /locus_tag="PKV23_10600"
                     /inference="COORDINATES: protein motif:HMM:NF005741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HQX85929.1"
                     /translation="MLDLSTNYLGLALKNPLVPSSSPLSHNIDSMLHLEDAGAAAIVM
                     HSLFEEDVRNDERMIDRFLINPDAFGEAAGHIPVGRDYQSKLDGYLEQIQLLKSRLSI
                     PVVASLNGSSLSGWVELGKEMQVAGADALELNAWFMPGDPKMCGEALEQHYLALLQEL
                     KAAVNLPISMKLSPFFSSLPHFVHQAEIAGAAGVSLFNRFFQPDIDLETLRVVDRVQL
                     SSSADTLLTMRWIAILRGRSSLTLAASGGVHTAEDALKMLLAGADVVHMASAVLQHGP
                     QALKEVLIGIERWMEVREYDSVTQLKGSMSQQNLPDPSAFARAAYLNALNSFTPPPGV
                     RY"
     gene            1330..>1574
                     /locus_tag="PKV23_10605"
     CDS             1330..>1574
                     /locus_tag="PKV23_10605"
                     /inference="COORDINATES: protein motif:HMM:NF013246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family ion channel"
                     /protein_id="HQX85930.1"
                     /translation="MPYIASRLILVLAVSVISVSCRDWWLNEHAASSLSIPPFTLMGI
                     SLAIFLGFRNTVSYERYWEARKQWGALLIAARALTRQV"
BASE COUNT          303 a          482 c          438 g          351 t
ORIGIN      
        1 ccaaaaaaga tgcccggctg cgccatcagg aataccttga actcgccgaa atggctgtgc
       61 cggaagtggt catcgcggaa aaagaccgcg aggctgccga attgagcgcc gtacagaagg
      121 agacacccaa tgcttgatct ctcgacaaat tatctcggcc tggcgctgaa aaacccgctc
      181 gtcccatcct cgtcgccgct cagccacaac atcgactcga tgttgcacct ggaagacgcc
      241 ggtgccgctg ccatcgtcat gcattcgctg tttgaggaag atgtccgtaa cgacgaacgg
      301 atgatcgacc gctttctgat caatcccgat gccttcggcg aagcggcagg tcatatccct
      361 gtcgggcgcg attaccagag caagctcgat ggctatctcg aacagattca actactgaaa
      421 tcgcggctga gtatcccggt tgtcgccagt cttaatggtt catcactgag cggctgggtc
      481 gagcttggaa aggaaatgca ggtggctggc gccgatgcgc tggaactcaa tgcctggttc
      541 atgcccggcg atccgaagat gtgtggcgag gcgcttgagc agcactacct cgcactgctc
      601 caggagttga aggctgcggt caacctcccg atcagcatga aattgtcgcc cttcttttcc
      661 tcgcttccgc atttcgtgca ccaggccgaa atcgccgggg cggccggtgt ttcgctgttc
      721 aaccgcttct tccagccgga tatcgacctg gaaaccctgc gcgttgtgga ccgcgtccag
      781 ctctccagtt cggctgacac cttgctcacc atgcgctgga tcgccatcct gcgtggccga
      841 agcagtttga ccctggctgc cagcggcggt gtgcacacgg cggaagacgc cctgaaaatg
      901 ctgctcgctg gcgccgatgt cgtgcacatg gccagcgccg ttctgcaaca cggcccgcag
      961 gcgctgaagg aagtgctgat cggcatcgag cgttggatgg aagtacggga atatgattcg
     1021 gtaacgcagc tcaaaggctc aatgagccag caaaacctgc ccgacccaag tgcctttgcc
     1081 cgggcggctt acctgaatgc actcaattcc tttacgccgc cgccgggtgt acgctactaa
     1141 gcggctttgc ccgatgctca gcctttctcc ttccaatgct ggcggtcaac ctccgccagc
     1201 agcctaaatg tgaagcccgc ttttaatgaa taaaatgctt gtgccgcttc aaacccggct
     1261 tctttgccca tgatcgtccg cccgcatctg cattggtttc gtctgctctt cgtttggcgg
     1321 ggctcggtat tgccgtacat cgcctcccgc ctgattctcg ttctggcggt ttccgtgatc
     1381 agcgtgtcgt gtcgggactg gtggctgaac gaacatgccg cctcatcgct ctcgattccg
     1441 ccctttaccc tgatgggcat ttcgctggcc atcttcctcg gctttcgcaa tacggtcagt
     1501 tacgaacgct attgggaagc ccgcaaacaa tggggtgcgc tactcattgc ggcacgggcg
     1561 ctgacccggc aggt
//
LOCUS       DAORVN010000591         1574 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_93918_length_1574_cov_2.904542, whole genome shotgun sequence.
ACCESSION   DAORVN010000591 DAORVN010000000
VERSION     DAORVN010000591.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93918_length_1574_cov_2.904542"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            173..802
                     /locus_tag="PKV23_10610"
     CDS             173..802
                     /locus_tag="PKV23_10610"
                     /inference="COORDINATES: protein motif:HMM:NF020905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1989 domain-containing protein"
                     /protein_id="HQX85931.1"
                     /translation="MGQATIILDEVVPAGKPWGARLAAHDVLTIIDLEGQQAVDFLCY
                     DAANPQDRYCATNTVKVQGNIFVSLGSVLYSDSGAALMTVIEDTVGRHDTIYGCCSNP
                     NNFLRYGVHTTESCYSNFETILQRFGLGPSAIVPNVNWFMSVPVLEDGSAGVTTAALN
                     PGSHVALRAERDVLAVLSNCPQMHNPCNGYNPTPIRVTVTRHRAKGKEE"
     gene            807..>1574
                     /locus_tag="PKV23_10615"
     CDS             807..>1574
                     /locus_tag="PKV23_10615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQX85932.1"
                     /translation="MTETTKHDAWSAGDSYDAYMGRWSRKMAARFLDWLKPAENLDWL
                     DVGCGTGALSSVVLSRCHPRSLVGIEQSAGFVATAQRNIDDKRARFEVGDAQNLPLDD
                     HSRDIVASALVLNFVPDIPKALSEMKRVVRPGGTVAFYVWDYPGGGMGFMRAFWNAAI
                     ALDPAVASDFAEGKRFPFCTAAGLSELVTNAGLQSVECTAIEIPTIFADFDDFWRPFT
                     LGAGPAPGYCVSLPEEAREKLRQKLSDDLPRQDNGTIT"
BASE COUNT          310 a          485 c          463 g          316 t
ORIGIN      
        1 gcccgcgcgc cggccgcgtc gtcgaggcgt tcaagaaata cgccgacgtc atgcaaaaag
       61 ccggctaccc ctatcgctga tgggagcagg tttcagttag aaggagcggc tccatcgggg
      121 ccgctctttt tccactgatc acgttccgtc gttctgaaag gcgggaactg cgatgggcca
      181 ggccacgatc attctcgatg aagttgtccc ggctgggaag ccctggggtg cgcgccttgc
      241 agcccatgac gttctcacca tcatcgacct cgaaggccag caagccgtcg attttctctg
      301 ttacgacgcg gccaatccgc aggaccgcta ctgcgccacc aacacggtga aggtccaggg
      361 caatatcttt gttagccttg gcagcgtgct ttattccgac agcggtgccg ccctgatgac
      421 ggtgattgaa gatacggtag gccggcacga cactatttat gggtgttgca gcaaccccaa
      481 caatttcctg cgctacggcg tccacacgac ggagtcctgc tactcgaact tcgagacaat
      541 cttgcagcgc ttcggtctcg gcccctcggc catcgtgccc aacgtgaact ggttcatgag
      601 cgttccggtc cttgaagatg gcagcgccgg ggttaccacc gcggcactca atcccggaag
      661 ccatgtggcg ctgcgggccg agcgcgatgt gctggccgtg ctttccaatt gcccgcaaat
      721 gcacaatccc tgcaacggct acaacccaac gcccatccgc gtgaccgtca cgcgccatag
      781 ggcaaagggg aaggaggaat agcccgatga cagaaacgac caagcatgac gcgtggagcg
      841 cgggcgacag ctacgatgcc tatatggggc gttggagcag aaagatggcg gcgcggtttc
      901 ttgactggct gaagccggcc gaaaaccttg actggctcga tgtgggctgc ggcaccggag
      961 cactttcatc ggtggttctc agccgctgcc atccccgcag tcttgtgggc atcgagcagt
     1021 ccgccggatt tgtcgccacg gcgcaacgaa atattgatga caagcgcgcc cggtttgaag
     1081 tgggcgatgc gcaaaacctg ccgctcgatg atcacagccg ggacatcgtg gcctccgccc
     1141 ttgtcctgaa ttttgtgccg gacattccaa aggccttatc tgaaatgaaa cgcgtggtgc
     1201 ggcccggcgg caccgttgca ttctatgtct gggactatcc cggcggcggc atgggattca
     1261 tgcgggcttt ctggaatgcc gccatagctc ttgatcccgc cgtcgcatcc gactttgccg
     1321 agggcaaacg gtttcccttt tgcactgccg ccggtctttc cgaactggtt acgaatgccg
     1381 gcctgcagtc agtggaatgt acggccatcg aaattccaac catctttgcg gatttcgacg
     1441 acttctggcg cccctttacg cttggcgccg ggccagcccc cggctattgc gtcagcctcc
     1501 ccgaggaagc gcgcgaaaaa ctgcgccaga aactgagcga tgatctgccg cgccaggaca
     1561 acggcacgat aacc
//
LOCUS       DAORVN010000592         1571 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_94241_length_1571_cov_2.649077, whole genome shotgun sequence.
ACCESSION   DAORVN010000592 DAORVN010000000
VERSION     DAORVN010000592.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1571
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94241_length_1571_cov_2.649077"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1571)
                     /locus_tag="PKV23_10620"
     CDS             complement(<1..>1571)
                     /locus_tag="PKV23_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006203360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQX85933.1"
                     /translation="KYLGLETEMMSMKEAKALNPLLEEKYFVGAMLDAADGNLDPEGT
                     THAYAKSARIAGAEIYQNCRVTDLKARADGTWDVITVNGNIHAEHVVNCGGLWAREVG
                     RMVGLELPVLAMEHMYLVTDDMPEVIAYNKERGRELPHIIDFKSEIYMRQERSGIVLG
                     TYEKECKPWQPKQTPWDFGQELLPPDLDRIADHLEIGYRHFPALAKVGIKRVINGPFT
                     FSPDGNPLVGPVQGLKNFWCACAVMAGFSQGGGVGLALSQWMVNGDPGFDVWAMDVSR
                     FGEWATRSYTNEKVRENYARRFSIKFPNEELPGARPQQTTALYDTMIAQGAVMGDSWG
                     LETPLWFAPKGVEPKDIFSFHRSNDFAHVKAEVLGVRNGVGVTEIANFAKYRFTGHGA
                     EAFLSRLMTNKMPKVGRLILTPMLNPAGKLIGDFTIANAGNDTFYMWGSSQAQKYHMR
                     WFEQHVPNDGSVNIHRYDMGLVGLSIAGPKSRAVLQKLTDADVSNAAFKFMDHRAMDI
                     GNVPALINRVTYTGD"
BASE COUNT          311 a          499 c          440 g          321 t
ORIGIN      
        1 gatcgcccgt ataggtgacg cggttgatca gcgccggcac attgccgata tccatggcgc
       61 ggtggtccat gaatttgaag gcggcgtttg aaacatcggc gtccgtgagc ttctgcagga
      121 ccgcacggga ttttggccca gcgatggaaa gcccgacaag ccccatgtca tagcggtgaa
      181 tgttcacgct gccgtcattc ggcacatgct gctcgaacca gcgcatgtga tatttctgcg
      241 cctggcttga gccccacata tagaaagtgt cattgcccgc attggcgatg gtgaaatcgc
      301 cgatcagctt gccggcagga ttcagcatcg gcgtcaggat caggcggccc accttcggca
      361 tcttgttggt catcaggcgc gagaggaagg cttccgcccc gtgcccggtg aaacggtact
      421 tggcgaaatt ggcaatttcg gtgacgccca cgccattgcg cacaccgaga acttccgcct
      481 tcacatgggc aaagtcattg gagcggtgga aggagaaaat gtccttgggc tcaacgcctt
      541 tcggcgcaaa ccacagcggc gtttcaagcc cccaagaatc acccatgacg gcgccttgcg
      601 caatcatcgt gtcataaagc gccgtggtct gctgcggccg cgcccccggc aattcctcat
      661 tcggaaactt gatggagaag cgccgcgcat aattctcccg cactttttca ttggtgtagc
      721 tccgcgtcgc ccattcgccg aagcgcgaca catccatggc ccagacgtca aagcccggat
      781 cgccgttcac catccactgc gaaagcgcca gccccacccc gccgccctgg ctgaagcccg
      841 ccatcaccgc gcaggcgcac cagaaatttt tgagcccttg caccggcccc accagcggat
      901 tgccgtcggg cgagaaagtg aaaggtccgt tgataacccg cttgatgcca accttggcca
      961 acgccgggaa atgccggtag ccgatttcca gatggtcggc gatgcggtcg agatccggcg
     1021 gcagaagttc ctggccaaaa tcccacggcg tctgtttggg ctgccatggc ttgcactctt
     1081 tttcataggt gccgagcacg atgccggagc gctcctggcg catgtagatt tccgatttga
     1141 aatcgatgat gtgcggcaac tcgcggccgc gctccttgtt gtaggcgatg acctccggca
     1201 tgtcgtcggt caccagatac atatgctcca tcgccagcac cggaagctcc agccccacca
     1261 tgcggcccac ttcgcgggcc catagcccgc cgcagttcac cacatgctcc gcatggatat
     1321 tgccgttcac cgtgatcaca tcccaggtgc catcagcgcg cgccttaaga tcggtgacgc
     1381 ggcaattctg gtagatctcg gccccggcaa tccgcgccga cttggcatag gcatgggtcg
     1441 tgccctcggg atcgaggttt ccgtcggcag catcaagcat ggcgccgacg aaatacttct
     1501 cttccagaag cgggttcagc gccttggctt ccttcatcga catcatctcg gtctcaagcc
     1561 cgagatattt g
//
LOCUS       DAORVN010000593         1570 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_94385_length_1570_cov_1.577558, whole genome shotgun sequence.
ACCESSION   DAORVN010000593 DAORVN010000000
VERSION     DAORVN010000593.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1570
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94385_length_1570_cov_1.577558"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>599)
                     /locus_tag="PKV23_10625"
     CDS             complement(<1..>599)
                     /locus_tag="PKV23_10625"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HQX85934.1"
                     /translation="GGLLRGASLTPGNEIVMGGDGLATGELREAAAFAPLLGLTATGG
                     REWLGMVTGENPVPPATSAERLIDQNFMKRGLEYCASLGITSIHNMDGNWYQLELLKA
                     LMDRDELIARVEVPFHQKNYFETSRVDEAVEMRAQYQGDMLHSGRVKIFMDGVLESLT
                     ALMLEDYPGFPGNRGAPLFTAEQFNEVATRADRHGLQIS"
     assembly_gap    600..609
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<610..1107)
                     /locus_tag="PKV23_10630"
     CDS             complement(<610..1107)
                     /locus_tag="PKV23_10630"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HQX85935.1"
                     /translation="MPSADLIITNARILTMNKQALRAESLAIAGNRILAVGSNADVKA
                     LKGSHTRVIDAQMNTVMPGIVESHIHLFGGAVELDSLMINNIKGLPAVSAAIREYAKA
                     NPKDRIIIVNGAAPVSLGDTTAITRQLLDTIIADRPLALICFDHHTVWANTKALETAG
                     LLHGAS"
BASE COUNT          292 a          429 c          488 g          351 t
ORIGIN      
        1 cgctgatctg gagcccatgc ctgtcggcgc gggtggcgac ttcgttgaat tgctccgccg
       61 tgaagagagg tgctccgcga ttgccgggga agccggggta atcctcgagc atcagggcgg
      121 tgagggattc gagcacgcca tccatgaaga ttttcacccg gccggaatgc agcatgtcgc
      181 cctgatactg ggcccgcatt tcaacggctt catcgacgcg ggaggtttcg aaataattct
      241 tctggtggaa aggcacctcg acgcgggcga tgagttcatc cctgtccatg agcgccttca
      301 gcagctcaag ctgataccag ttgccatcca tgttgtggat ggaggtgatg ccgagggagg
      361 cgcaatattc cagcccgcgc ttcatgaaat tctggtcgat gaggcgctcg gctgatgtgg
      421 ccggcggcac gggattttca ccggtgacca tgccgagcca ttcgcgcccg cccgtggcgg
      481 tgaggcccag cagcggggca aaggctgccg cctcgcggag ttcgccggtg gccagcccgt
      541 cgccgcccat gacgatttca ttgccggggg tgaggctggc gccacgcaac agcccgccgn
      601 nnnnnnnnng ctggcgccat ggaggaggcc tgccgtttca agcgccttgg tattggccca
      661 gaccgtgtgg tggtcaaagc agatgagggc caggggcctg tccgcgatga tggtgtccag
      721 aagctggcgg gtgatggcgg tggtgtcgcc cagggacacg ggggcggcac cattgacgat
      781 gatgatgcgg tccttcgggt tggccttggc atattcacgt atcgctgccg aaacggctgg
      841 caagcccttg atgttattga tcatcaggct gtcgagctcg accgcgccgc caaacaggtg
      901 gatgtggctt tcgacgatgc cgggcatcac cgtattcatt tgggcgtcga taacacgggt
      961 gtgactgccc ttcagggctt tcacgtcggc gttggagccc acggccagaa tgcggttgcc
     1021 ggcgatggcc agcgattcgg cccgcagggc ctgcttgttc atggtgagaa tacgggcatt
     1081 ggtgatgatc agatcggcag atggcatgat ttttccctgt tttgcgctgc ccaaaatgca
     1141 ggcttgcaat ccgttccgca agcctccaca tttggccggt cttcaacaat tgaaaggagg
     1201 tgatccgatg tcgagtgagt tcacagcgta tgccgtcagg ctcgtgcgga tcgaattctt
     1261 ggcaggggtt actgcctgat cgatctcata ccgggatgct gatggaacgc atctcacgga
     1321 agaggccgct ttgcagcggc ctcttttttt gagggaattg ttcccttttt ccttcgctga
     1381 gccgctcgtg aactgattgc tacatccaaa agtaagttgc cgaattgcac tgatccacca
     1441 accaattctt tcacctgctt gcgaacgcct tcgttcaaat ggcatatatc tagccgtcag
     1501 agttgactga tttcagacca accgtctttg ctaagaaccg agcctacatg agcacactga
     1561 gagagccttc
//
LOCUS       DAORVN010000594         1568 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_94516_length_1568_cov_3.502313, whole genome shotgun sequence.
ACCESSION   DAORVN010000594 DAORVN010000000
VERSION     DAORVN010000594.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1568
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94516_length_1568_cov_3.502313"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..841
                     /locus_tag="PKV23_10635"
     CDS             <1..841
                     /locus_tag="PKV23_10635"
                     /inference="COORDINATES: protein motif:HMM:NF019122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85936.1"
                     /translation="DLILLGVSSAGVMWAIERLSETLGKPIPVLMITKGMHPEAASLA
                     TLPDLVAKSLKQNLGFDVPVAAIGGPCIAGELAVRRLTGTVIVSHDAALAAQLCAALE
                     TDYYHPRPSLDMVGVETCAAFKNFFAIAVGWAHGALARMQPAENKAQNNNAAAVIFDQ
                     SVREMMALTRALGGSAESVWGMPGVGDLYVTCQAGRNSRLGNNLGRGLTYSEARNGPM
                     KGDTIEGAELGVATASSLRAMMADGVLDDSAMPLASALLKTLVSDAPLEIPWKVLHRA
                     GNT"
     gene            838..>1568
                     /locus_tag="PKV23_10640"
     CDS             838..>1568
                     /locus_tag="PKV23_10640"
                     /inference="COORDINATES: protein motif:HMM:NF016114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding domain-containing protein"
                     /protein_id="HQX85937.1"
                     /translation="MRSGVVEIDLPSTEDLSGFDPEEQLATRAAWLYFVGSNTQAEIG
                     KKLGLTRVRINRLLALAREQGLVQVNVTGRLADCVALEEMLKERFKLQHAVVVPTPLN
                     RSQLPYVIGNAAGHYLGSQLKDGMSVGVGWGRTLRYSLRSVPRRLYSNLSVVSLMGGL
                     TQASLVNPHETASHLADIVGAQCYYIAAPALTDSEATRDIFLEQSMVRDVFERGSKVD
                     LALLSVGELTHPNTVTQVGLISNAEA"
BASE COUNT          300 a          448 c          509 g          311 t
ORIGIN      
        1 agacctgatt ttgcttggtg tatcttcggc cggggttatg tgggcaattg aacggctctc
       61 tgaaacgctg gggaagccca tacccgtatt gatgattacc aagggcatgc acccggaggc
      121 tgcgagcctt gcaacattgc cggatctggt cgccaaatcg cttaagcaga atttgggatt
      181 tgatgtgccc gtggccgcca tcggcggccc ctgcattgcc ggcgaacttg cggtgcgccg
      241 tctgacgggc acggtcatcg tttcgcatga cgcagcattg gcagcgcagc tctgtgccgc
      301 ccttgaaacg gattattatc acccacggcc ctcgctggac atggtggggg ttgaaacctg
      361 tgcggccttc aagaacttct ttgccattgc ggtgggctgg gcccatggcg ccttggcgcg
      421 catgcagcct gcggaaaaca aggcgcaaaa caacaatgcc gcagcggtga tttttgacca
      481 gtcggtgcgg gagatgatgg cgcttaccag ggcgctgggc ggcagtgcgg aaagcgtttg
      541 gggcatgccg ggggttggcg acctctatgt cacctgccag gcggggcgca attcccggct
      601 cggcaacaat ctgggacgcg gcctcaccta cagcgaagcc cggaatgggc cgatgaaagg
      661 cgacaccatc gaaggcgcgg aacttggcgt ggcaacggcc agttcgctcc gcgccatgat
      721 ggccgacggg gtgctggatg atagcgccat gccgctggca agcgccctgt tgaagacgct
      781 ggtttcggac gcgccgcttg aaattccgtg gaaggttcta caccgggcag gaaacacatg
      841 agatcaggag tcgtggaaat cgatctgccg agtacggagg acctttcagg attcgatcct
      901 gaggagcagc tcgccacgcg ggcagcctgg ctctattttg ttggcagcaa tacgcaggcg
      961 gaaatcggca agaaactggg gctcacccgg gtgcgcatca accgcctgct agccctggcg
     1021 cgcgaacagg gcctggtcca ggtcaatgtc acaggccggc tggcggactg cgtcgccctg
     1081 gaggaaatgc tcaaggagcg gttcaagctg caacacgccg tcgtcgttcc cacgccgctt
     1141 aaccgaagcc agcttcccta tgtcattgga aatgcggcgg ggcactatct gggatcgcag
     1201 ctcaaggacg gcatgtcggt gggggtgggc tgggggcgca cgctgcgcta ttcgctgcgt
     1261 tcggttccac ggcggctgta cagcaatcta tccgtcgttt cgctgatggg cggactcacg
     1321 caggcctcgc tggtcaatcc gcatgaaaca gcctcgcatc tggccgatat cgttggcgcg
     1381 caatgctatt acattgccgc tccggcgctc acggattcag aggcaacgcg ggacatattc
     1441 ctggaacagt ccatggtccg ggatgtgttc gaacgcggct caaaggtcga cctggcgctt
     1501 ctctcggttg gcgaactcac ccatcccaat acggtgacgc aggttggcct gatctccaat
     1561 gcggaagc
//
LOCUS       DAORVN010000595         1568 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_94559_length_1568_cov_2.437541, whole genome shotgun sequence.
ACCESSION   DAORVN010000595 DAORVN010000000
VERSION     DAORVN010000595.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1568
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94559_length_1568_cov_2.437541"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(111..728)
                     /locus_tag="PKV23_10645"
     CDS             complement(111..728)
                     /locus_tag="PKV23_10645"
                     /inference="COORDINATES: protein motif:HMM:NF017815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like cysteine peptidase"
                     /protein_id="HQX85938.1"
                     /translation="MRFRSSVISGAIACLFSVNSALAFGPGPKDAAPEFGKTLPPVGF
                     VKFCVTNPADCKTRNAAHRRIAMTPERWNQLYVVNSYVNGKVAPISDQDLYGEAEHWA
                     YPTDAGDCEDYVLLKKRQLEGLGFSPEALLITVVLDEKNEGHAILTVTTDAGDYVLDN
                     RFDKVSRWNDTKYKFLKRQSHANPLQWVALMKQSTSTSGFVASGN"
     gene            complement(832..>1568)
                     /locus_tag="PKV23_10650"
     CDS             complement(832..>1568)
                     /locus_tag="PKV23_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017266968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HQX85939.1"
                     /translation="VDLPVPDYEKAIGKSVKGLRVGIPKEYRADGMSAEIEALWDKGA
                     QWLKEQGAEIVEISLPHTKYALPAYYIVAPAEASSNLARYDGVRYGLRVPGDDIVDMY
                     ENTRAAGFGPEVQRRILIGTYVLSAGYYDAYYVRAQKIRTLIKRDFEQAWSKVDVVLT
                     PATPSPAFAPGEISDPVQMYLNDIFTVTVNMAGLPGIAVPAGSSKEGLPLGLQLIGKA
                     FDEETLFRTAAAIEQVAGGYHAPKWW"
BASE COUNT          331 a          441 c          458 g          338 t
ORIGIN      
        1 aagactttga agccctattt gcgcctggtg gcgcagccgg gaaaataaat ccggttatct
       61 taatctcccg caccaccctg ccccggagca accctccggg gtttttcttg ttagttgccg
      121 ctcgccacaa agccgctggt ggaggtggat tgcttcatca gcgctaccca ttgcagcgga
      181 ttggcgtggg actggcgctt caggaatttg tacttggtgt cgttccagcg cgagaccttg
      241 tcaaagcggt tgtccaggac gtaatcgccg gcgtcggtgg taacggtgag tatggcatgg
      301 ccctcgttct tctcatcgag gacaacggtg atgagcagcg cctctggcga aaaccccaac
      361 ccctcgagct gacgcttttt gaggagcacg taatcttcgc agtctccggc atcggtagga
      421 taggcccagt gttcggcttc gccgtagaga tcctggtcac tgatcggagc aaccttgcca
      481 ttcacgtagc tgttcaccac gtagagctgg ttccagcgtt ccggagtcat ggcgatccgc
      541 cggtgagctg catttcgcgt tttgcagtcg gccggattgg tcacacagaa tttcacgaaa
      601 cccacgggcg gcaaggtctt gccaaattcc ggggcagcat cttttggtcc aggcccgaag
      661 gccaaagcgc tgttaacgct gaacaaacac gctattgcac cagaaataac cgaagaccga
      721 aaccgcatat tgccccagaa ccacattttg ccctgaagca aggtaaatca aaatttgcga
      781 ggcggggaaa cataattaac aaatagttaa cgcaggtgca gggctgggtt cttaccacca
      841 cttcggggcg tgatagccgc ctgccacctg ctctatggcc gccgccgttc tgaacaaagt
      901 ctcttcatca aaagccttgc cgatgagttg aaggcccaag ggaaggcctt ccttggaact
      961 cccggccgga acggcgatgc cgggaagtcc cgccatgttc acggtgacag tgaaaatgtc
     1021 gttcaggtac atctgcaccg gatcgctgat ttcgcccggc gcaaaggcgg gacttggtgt
     1081 ggccggagtg agcacgacat caaccttgga ccaggcctgt tcgaagtcac gcttgatcag
     1141 tgtgcggatt ttctgggcgc ggacgtaata ggcatcatag tagccggcgg acaggacata
     1201 ggtgccgatc aggatgcggc gctgcacttc ggggccgaag ccggcggcac gggtattttc
     1261 atacatgtcc acaatatcat cacccggaac gcgcaagcca tagcgcacgc cgtcatagcg
     1321 ggcgaggttt gacgaggctt cagcgggcgc cacgatgtaa taggcgggga gcgcgtattt
     1381 cgtgtggggc agcgagattt caacgatctc ggcgccctgc tctttcagcc actgggcgcc
     1441 cttgtcccac agggcttcga tttcggcgga catgccgtcg gcgcggtatt ccttcggaat
     1501 gccaacgcgc aggcccttga cggatttgcc aattgccttt tcataatcgg gcaccggaag
     1561 atcgaccg
//
LOCUS       DAORVN010000596         1566 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_94764_length_1566_cov_2.979484, whole genome shotgun sequence.
ACCESSION   DAORVN010000596 DAORVN010000000
VERSION     DAORVN010000596.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1566
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94764_length_1566_cov_2.979484"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..484
                     /locus_tag="PKV23_10655"
     CDS             <1..484
                     /locus_tag="PKV23_10655"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006612599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HQX85940.1"
                     /translation="AIIQGREERLPVDQRTPIPGATWHPTTGTGVPLMVFASPIRELA
                     AEGKLTLAFGIGINTARVVAGNMGSHTRLNYTVIGDGVNLASRLETMTKDPAHRTSII
                     ISEATARAAGEESKLRPLGAVTVKGKTEAVQVFAVDNQPRPWPTRPLARPPTQPPYHS
                     "
     gene            complement(475..1059)
                     /locus_tag="PKV23_10660"
     CDS             complement(475..1059)
                     /locus_tag="PKV23_10660"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HQX85941.1"
                     /translation="MGPQLVGRETLRQAVVAWRGALVAGVAAGHHSAAAKLKQVAMAG
                     RWGILRVMPSEFSHTRQVEFHETDMAGIVHFANFFRWMEGCETAFYRSLGLPLISFVP
                     GQVVGWPRVNVSCAYRAPLRFNDTVVVRLFVKKLTTRSVTYVFQFWKDGTLCATGEVT
                     AVCVTADAQGRMVARPIPADVRAQLQEAPVGAYE"
     gene            1058..>1566
                     /locus_tag="PKV23_10665"
     CDS             1058..>1566
                     /locus_tag="PKV23_10665"
                     /inference="COORDINATES: protein motif:HMM:NF025729.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4412 domain-containing protein"
                     /protein_id="HQX85942.1"
                     /translation="MKLLSRLLAVGALLTAVQVFAAGTFEGKVSLAITGNKGRPMNMD
                     YSLKGQKVRMDMHAEGNQVASIMDLAKLEMLMLMPDQQMYMVMPIKKPVESAMAKQEE
                     SSADIEVTGKTETILGYKCSQILVKDKGAVTEVWVAEGLGMFMGLGGGPMGGGMMGGR
                     KAANAAKWE"
BASE COUNT          309 a          531 c          476 g          250 t
ORIGIN      
        1 ggcgatcatc cagggaaggg aagagcgctt acctgtcgat cagcgcacac cgatcccagg
       61 ggcaacctgg cacccgacta ccggcactgg cgttcccctc atggtctttg cttctcccat
      121 ccgcgaactc gcggccgagg gcaaactgac gctcgccttc ggcatcggca tcaataccgc
      181 gcgggtcgtc gcgggcaaca tgggttccca cacccggctc aactacaccg tcatcggcga
      241 tggcgtgaac ctggcttccc gcctggagac catgaccaag gacccggcgc accggaccag
      301 catcatcatc agcgaggcca cggcccgcgc tgcgggcgag gaatcgaagt tgcgcccact
      361 cggtgccgtg accgtgaagg gcaagacgga ggccgtgcag gtcttcgctg tggacaacca
      421 gccccgcccc tggccgaccc gcccgctggc gcgtccgccg acccagccgc cttatcactc
      481 gtaagccccg accggcgctt cctggagttg cgcccggacg tctgcgggga tcggccgtgc
      541 gaccatcctc ccctgggcgt cggccgtcac gcacaccgcc gtcacctcgc cggtggcgca
      601 cagcgtgccg tccttccaga attgaaagac ataggtcacc gagcgggtgg tgagtttttt
      661 cacgaaaagc cgcaccacga ccgtgtcgtt gaaacgaagt ggcgcgcggt aggcgcaact
      721 gacattcacc cgcggccagc cgacgacctg ccccggcacg aaggatatga gcggcaatcc
      781 cagcgagcgg tagaaagccg tctcgcaccc ttccatccag cggaagaagt tggcgaaatg
      841 cacgatgccg gccatgtcgg tctcgtggaa ctcgacctgc cgcgtgtggc tgaactccga
      901 gggcatgacc cgcagaattc cccaccgccc cgccatggca acttgtttca gcttggcggc
      961 ggcggagtgg tgcccggcgg caactcccgc caccaaagct ccacgccatg caaccacggc
     1021 ttgcctcagt gtttcccgcc ccacaagctg cggccccatg aaacttcttt cccgtcttct
     1081 cgccgtgggc gcactgctca ccgctgtcca ggtttttgcc gccggcacct tcgaaggcaa
     1141 agtcagcctc gccatcaccg gcaacaaggg ccgccccatg aacatggatt attccctcaa
     1201 gggccagaag gtccgcatgg acatgcacgc cgagggcaac caggtcgcct ccatcatgga
     1261 tctggccaaa cttgaaatgc tgatgctgat gcccgatcag cagatgtata tggtcatgcc
     1321 gatcaagaag ccggtcgagt cggccatggc caagcaggag gaaagcagcg ctgacatcga
     1381 agtcacgggc aagaccgaga ccattctcgg ctacaagtgc agccaaattc tcgtaaagga
     1441 caagggcgcg gtcacggagg tatgggttgc cgagggactg ggcatgttca tgggcctggg
     1501 cggcggcccc atgggtggcg gcatgatggg cggccgcaag gcggccaacg ccgccaaatg
     1561 ggagga
//
LOCUS       DAORVN010000597         1565 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_94856_length_1565_cov_3.495364, whole genome shotgun sequence.
ACCESSION   DAORVN010000597 DAORVN010000000
VERSION     DAORVN010000597.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1565
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94856_length_1565_cov_3.495364"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1166)
                     /locus_tag="PKV23_10670"
     CDS             complement(<1..1166)
                     /locus_tag="PKV23_10670"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQX85943.1"
                     /translation="MIQLPFMKQLLTIFFRAEGTRPWIVLACLLIASLSEALGISTLL
                     PAINTILGDNAADTPGIGRSIKVFIESMGITPNLGNLLIIIVSLLVLKSVIVFGALAY
                     SGITGARVAINLRRRLIKAIFDARWSFYAEESGGRFANAISNDATRAGDAYQFAAVVM
                     AGGLQLMTFSVVALLIDWRIAIVGGVAGLFIALVMSRLIRVLKQSGYKQTDRVSSLTV
                     DMVDMLNNIKALKSMDRYGTMVEGLSGILKRIKRSLITIHLAKNGVTQGSDALQNLMT
                     GVFIYTAYVLFGLSLPELLVTGIVFFQIVNNLTKLQKQLQAAVVVESAYVRTMELIKR
                     AEGEKEVHTGASAPDLGTGCRFEDVTFAHAKAPVVVSATFDIPANQITVLQGPSG"
     gene            1319..>1565
                     /locus_tag="PKV23_10675"
     CDS             1319..>1565
                     /locus_tag="PKV23_10675"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HQX85944.1"
                     /translation="MGTRTAVFDDENRVLLVRHSYGTGWLLPGGGVERGETLFESAKR
                     EIREEAGIIAQEEPVLQGICLNDELFPGDHVAVFTLRK"
BASE COUNT          338 a          421 c          477 g          329 t
ORIGIN      
        1 ccggacggtc cttgcagcac ggtgatctgg tttgcgggaa tatcaaatgt ggcgcttacc
       61 accactgggg ccttggcatg ggcaaaagtc acgtcctcga acctgcatcc cgtgccgaga
      121 tccggcgcgg aagcgcccgt atggacttct ttctctcctt ctgcccgttt gatcagttcc
      181 atcgtgcgca cataggcact ttcgacgacg accgccgcct gcagttgttt ttgaagtttg
      241 gtgaggttgt tcacgatctg gaaaaacacg atgccggtca ccagcaactc cggcaggctc
      301 aagccaaaca gcacataggc ggtgtagatg aagacccccg tcatcaggtt ttgcagggcg
      361 tcgcttccct gtgtcacgcc gttcttggcc agatggatgg tgatcaggct gcgcttgatg
      421 cgtttcagaa ttccggagag gccttcgacc atcgtgccat agcggtccat ggacttaagc
      481 gccttgatgt tgttcagcat gtccaccatg tccacggtga ggctggatac gcggtctgtc
      541 tgcttgtagc cggattgctt caggacgcgg ataagcctgc tcatcacgag cgcgatgaaa
      601 aggccggcca cgccgccgac gatggcgatg cgccagtcaa tcaggagggc gaccaccgaa
      661 aaggtcatga gctgcaggcc cccggccatg acaacggcgg cgaactgata ggcatcaccg
      721 gcgcgggttg cgtcattgct gatggcattg gcaaaacgcc cgccactttc ctcggcatag
      781 aaactccagc gcgcatcgaa gatggccttg atgagcctgc gccgcagatt gatggcgact
      841 cgggccccgg tgattccgga ataggcaagg gcgccgaaga cgataaccga cttcagcacg
      901 agcagggaca cgatgatgat cagaagattg ccaaggttgg gcgttattcc catcgattca
      961 atgaacacct ttatgctgcg gccgattcct ggcgtatcgg cggcattgtc acccagaatg
     1021 gtattgatgg cgggcaggag ggtgctgatg cccagggcct ccgacaggct ggcaatcaga
     1081 aggcaggcaa gcacgatcca gggccttgtc ccttcggctc tgaagaatat ggtgagaagc
     1141 tgcttcatga atggaagttg gatcacacga ttgagattgc cttgcgagca atagggccta
     1201 tgccttgcga actcaagctt gccacggtta actgcttgga atgataaaga gcattctcct
     1261 caaactatcc tcaaaggttg tgttgcagcc attatacagg caaacacgag gccttacgtt
     1321 gggcacgcga accgcggtat ttgatgatga aaatcgggtc cttctggtgc ggcactccta
     1381 cgggacgggg tggctgctgc caggcggcgg ggtcgagcgg ggcgagacac tgtttgaatc
     1441 ggcaaaacgc gaaatccgcg aagaggcggg aatcatcgcg caggaggagc cggtcctgca
     1501 gggcatttgc ctgaatgatg agctgttccc gggagaccac gtggcggtgt tcaccttgcg
     1561 caaat
//
LOCUS       DAORVN010000598         1564 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_94966_length_1564_cov_3.259112, whole genome shotgun sequence.
ACCESSION   DAORVN010000598 DAORVN010000000
VERSION     DAORVN010000598.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1564
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94966_length_1564_cov_3.259112"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(177..1286)
                     /locus_tag="PKV23_10680"
     CDS             complement(177..1286)
                     /locus_tag="PKV23_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008967700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQX85945.1"
                     /translation="MPPEATSPAPAENHAMLSEVSEIKEQTTLQRIFSSQAFWVTVAL
                     VVLYAGMAMYEVSFGTVDNFANMTRNFAPFGIMALGMTVVIITGGIDLSIGSIMGVVA
                     IVTGLFLSWEYSWYVAFFMGLAAGMACGAVNGFFVAYVGLSSFVVTLGMMSIARSLAV
                     VLSGNQMLYKFGPDAAIVKAIGQAKWPRQTPGDSIPDWIPQFSSHFWTMVVLGLIVGY
                     VFIFKAWGRHLFAIGGNEEAARLTGVPVNRVKFQAYVFSAFTASIASLLILGYSGSAI
                     NAMGQSYELRVIAATVIGGANLMGGAGTAFGAIVGSAFLEVIRNALLMAGIDSNWQGA
                     FVGAFIILAVLLGKQTGGKSFVAAMFDRLVPKRNR"
     gene            complement(1276..>1564)
                     /locus_tag="PKV23_10685"
     CDS             complement(1276..>1564)
                     /locus_tag="PKV23_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015007989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HQX85946.1"
                     /translation="VAIARTRLSNAKLVLMDEPTAAISVRQVAEVLELIRRLKAQGVA
                     IMLISHRMPDVFAVCDRIMVLRRGSLVADKATSKTSPEEITGLITGAIRAA"
BASE COUNT          349 a          466 c          483 g          266 t
ORIGIN      
        1 agcaggaccc cggtgttctt cggcaccagc gcgaaggtga acttcgcctg ggccccgacg
       61 gcggccacgg taagcgcccc aaaggcaacc gctgcggtca gtaaaaattt cttcatctca
      121 gatcctccac tttcgtttac gcagtgccgc cggatttatt tccagtcggc ggttttctag
      181 cggtttcgct tcgggacaag ccggtcaaac atcgcggcga cgaaagactt gccgccggtc
      241 tgcttgccga gaagaaccgc aagaatgatg aaggcgccaa caaaggcccc ttgccaattt
      301 gaatcaatgc cggccatcag cagggcgttg cggatgacct ccagaaaagc tgaaccgacg
      361 atggcgccga aggccgtgcc tgccccgccc atgaggtttg cgccgccgat cacggttgcg
      421 gcgatcaccc gcagctcata ggactggccc atggcgttga tggccgagcc gctgtagccc
      481 agaatgagca gcgaggcgat cgaggcggta aaggcggaga acacataggc ctggaacttc
      541 acgcggttga ccggaacacc ggtgaggcgt gcggcctcct cgtttccgcc gatggcgaac
      601 aggtggcggc cccaggcctt gaaaataaag acatagccga cgatgagacc aagcacgacc
      661 atggtccaga aatgcgatga aaactgcggg atccagtcgg gaattgaatc gcctggagtc
      721 tgccgcggcc atttcgcctg gccgatggcc ttgacgatgg cggcatcggg cccaaatttg
      781 taaagcatct ggttgccgga gagaaccacg gcaagcgagc gggcaatcga catcatgccc
      841 agcgtcacca cgaaggacga gaggcccaca taggcgacaa aaaagccgtt gaccgcgccg
      901 caggccatgc ccgccgccag tcccatgaag aaggcgacat accaggaata ttcccagctg
      961 aggaaaagcc cggtgacgat ggccaccacg cccatgatgg agccgatgga aagatcgatg
     1021 ccgccggtga tgatgaccac cgtcatgccc aaagccatga tgccgaaggg cgcgaagttc
     1081 cgggtcatgt tggcaaagtt gtcgacggtg ccgaaactca cttcatacat ggccatgccg
     1141 gcgtagagga caaccagcgc caccgtcacc cagaaagcct ggctggaaaa gatccgctgc
     1201 agcgtggtct gctccttgat ctccgaaacc tcggagagca tggcgtgatt ttccgcaggc
     1261 gctggtgagg tggcttcagg cggcacggat cgctcccgta atgagcccgg tgatttcctc
     1321 ggggcttgtc ttggaagtcg ccttgtcggc aaccagtgag ccgcgcctga gcaccatgat
     1381 gcggtcgcac acggcaaaga cgtcgggcat gcggtggctg atcagcatga tggccacccc
     1441 ttgcgccttg aggcggcgga tgagttcaag aacttccgcc acctggcgca ccgaaattgc
     1501 cgcggtgggc tcatccatca gcaccagctt ggcattggaa agcctggtgc gggcgatggc
     1561 cacg
//
LOCUS       DAORVN010000599         1564 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_94996_length_1564_cov_2.574553, whole genome shotgun sequence.
ACCESSION   DAORVN010000599 DAORVN010000000
VERSION     DAORVN010000599.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1564
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94996_length_1564_cov_2.574553"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..135)
                     /locus_tag="PKV23_10690"
     CDS             complement(<1..135)
                     /locus_tag="PKV23_10690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012112481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HQX85947.1"
                     /translation="MTRRDPNYIDVHVGSRIRMRRQLISMSQEKLGELLGITFQQVQK
                     Y"
     gene            complement(332..>1564)
                     /gene="lnt"
                     /locus_tag="PKV23_10695"
     CDS             complement(332..>1564)
                     /gene="lnt"
                     /locus_tag="PKV23_10695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HQX85948.1"
                     /translation="GMAVYWGLATFCVALSRREGLPVALVFAVSLAAFEYLRGVLFTG
                     FPWAAPGLASDGMGAVAQTASLWGMPALTLLIVLWAATWPFMWKGPRPHRAAALLIIA
                     TLPLCWAWGSYRLSQGAVANVDGVRIRIVQPNISQDSKWRDENARAIFYQLLEMSARA
                     APDGASPTHIIWPETAVTFLIDESEAAKVELAELLGGTKTLVTGALRRDINRSRDGEG
                     DKVFNSILTFDGSGKVVARYDKWRLVPGGEFLPFEWALEPLGFRKVVTVPGSFAAGPG
                     PVSLAVPGAPEAGFGVCYEAIFPDQFIDPQKRPGWLINVTNDGWFGNSTGPYQHLAQA
                     RLRSIEHGLPMVRAANTGISAVIDPYGRTLSSLPLNGEGIMDAGLPQSLPPTFFADYG
                     ELIFFTALLLLLALSFIL"
BASE COUNT          364 a          509 c          401 g          290 t
ORIGIN      
        1 atatttctgc acctgctgga aggtgattcc cagcagttcg ccaagcttct cctggctcat
       61 gctgatgagt tgccggcgca tgcgaatgcg cgacccgaca tggacatcga tgtaatttgg
      121 atctcgtctc gtcatattcc cctcagaccg ttgcccccgg cctgactctc gccatcgctc
      181 atttcaactg agaatagaca acagattccg tgatcaacat tctcttgaaa gattagtata
      241 atagttgtca gcctgtaaac aagaatcgct ggtaccagca gaaatccacc gacataacag
      301 ctgcttcggg catatcctcc atgaattcac attataagat aaagctcaac gccagcaaaa
      361 gtaaaagcgc ggtgaaaaat attaattcgc cgtaatctgc gaaaaaagtt ggcggcagac
      421 tctgcggcag ccccgcgtcc atgatgcctt ccccgttcaa tggcaaactt gacagcgtcc
      481 ggccataggg gtcgatcacc gccgatattc cggtgttggc cgcccgcacc atcggcagcc
      541 cgtgttcgat gctgcgcaag cgggcctggg ccagatgctg gtagggcccg gttgaatttc
      601 caaaccaccc gtcattggtg acattgatca gccagcccgg gcgcttctgc ggatcaatga
      661 actgatccgg gaatatggcc tcatagcaaa ccccaaagcc cgcttcgggg gcgcccggaa
      721 ccgcgaggct taccggcccc ggccccgccg caaaacttcc aggaaccgta accaccttgc
      781 gaaaccccag cggttcaagc gcccattcaa aaggcaggaa ctctcccccc ggcaccagcc
      841 gccacttgtc atagcgggcc acaaccttgc cggaaccgtc aaaggtcaga atgctgttaa
      901 agactttgtc gccttcgcca tcccttgacc tattgatgtc gcgccgcaag gcaccggtca
      961 ccagcgtttt ggtcccgccg agcaactcag ccaattcgac tttcgcagcc tcgctctcgt
     1021 cgatcagaaa ggtcacagcc gtttccggcc agatgatgtg ggtaggcgat gcgccgtccg
     1081 gcgcggcgcg ggccgacatc tcgagaagct gataaaaaat cgcccgggca ttctcgtccc
     1141 gccatttgga atcctgcgaa atgttgggct ggactattct gatgcgaacc ccatccacat
     1201 ttgcaacggc cccctgcgaa agccggtagc ttccccacgc ccagcacaag ggaagggtcg
     1261 caatgatcag cagggcggcg gcccggtgag gtcgcggtcc cttccacatg aatggccagg
     1321 ttgccgccca cagcacgatc agcaatgtca gcgccggcat gccccacagc gaagccgtct
     1381 gggcaaccgc ccccatgccg tcgcttgcca ggccaggcgc ggcccaagga aacccggtaa
     1441 acaggacacc gcgcagatat tcaaacgccg caagcgacac ggcaaaaacc agcgccactg
     1501 gaagcccctc ccgccggctc agggcaacgc agaaagtagc caatccccag taaaccgcca
     1561 ttcc
//
LOCUS       DAORVN010000600         1559 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_95521_length_1559_cov_2.478059, whole genome shotgun sequence.
ACCESSION   DAORVN010000600 DAORVN010000000
VERSION     DAORVN010000600.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1559
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95521_length_1559_cov_2.478059"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1176
                     /locus_tag="PKV23_10700"
     CDS             <1..1176
                     /locus_tag="PKV23_10700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008872393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyltransferase family protein"
                     /protein_id="HQX85949.1"
                     /translation="PLYDRLKKLNACFGSKLGWERPNWFAPAGEEARDIYTMGRQNWF
                     AAVGEEHRCVRERVGVFDQSSFAKYELRGKDAEEALGWICANNVARPVGKLTYTQMLN
                     SRGGIECDLTVARLAEDHFYIVTGTGFRTHDFAWIVDHLKPGLEAKLTDITEQYGTLS
                     VMGPKSRAVIQAVTKTDMSNAAFKFGEVREIEIAGHKVRALRVTYVGELGWELHMPIA
                     ATGEVFDALMVAGKPHGIAPVGYRALESLRLEKGYRAWSSDITPNDTPLHAGLGWAVK
                     MKSNQAFLGREAIEKIAQAPLTKMLAGFTTEREDIVLSGRETILRNGAFAGYLTSGGY
                     GYTIRKPVGFGYVRNADGVDEAYLEAGRYELVVAKEIVKANIHLQPIYDSLNQRVKS"
     gene            1173..>1559
                     /locus_tag="PKV23_10705"
     CDS             1173..>1559
                     /locus_tag="PKV23_10705"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006725447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQX85950.1"
                     /translation="MNWDRKVHILIIGGGAIGTSLAYHLGRDGAKDVLLVEKAQLTEG
                     CTWHAAGLVGQLRGKKNLTRLMQNSVAVFDRLEAETGQAISWKKVGSLRLASSAARWS
                     EIQRSMGQAKSFGVECQSLSAAEAKTL"
BASE COUNT          321 a          402 c          553 g          283 t
ORIGIN      
        1 ccgctttatg accgcttgaa aaaactcaat gcctgctttg gatccaagct gggctgggag
       61 cggcccaact ggtttgcgcc tgctggcgaa gaggcgcgcg acatctacac catggggcgg
      121 cagaactggt ttgcggcggt gggcgaggag caccgttgcg tgcgcgagcg cgtgggcgtg
      181 ttcgaccaat catcctttgc caagtatgaa ctgcgcggga aagacgcgga ggaggcgctg
      241 ggctggattt gcgccaataa cgtggcgcgg ccggtgggca agctcaccta tacgcagatg
      301 ctgaattcgc gcggcggcat cgaatgcgac ctgacggtgg cgcggctggc ggaggatcat
      361 ttctatatcg tgacgggcac cggcttccgg acccatgact ttgcctggat cgtggatcac
      421 ctcaagccgg ggctggaggc gaagctgacg gacatcaccg agcagtatgg aacgctttcg
      481 gtgatggggc ccaagtcgcg ggcggtgatt caggctgtaa caaaaactga tatgtcgaat
      541 gccgcattca aattcggcga ggtgcgggag atcgagattg cggggcacaa agtgcgggcg
      601 ctgcgggtga cctatgtggg cgagctgggc tgggaactgc acatgcccat tgcggccacg
      661 ggcgaggttt tcgatgcgct gatggtggcg ggcaaaccgc atggcattgc gccggtgggc
      721 taccgggcgc tggaatcgct gcggcttgag aagggctacc gggcctggag ttcagacatc
      781 acacccaatg atacgccact gcatgcgggg ctgggctggg cggtgaagat gaaaagcaac
      841 caggctttcc tggggcgcga ggcgattgaa aaaatcgcgc aggcgccact cacaaaaatg
      901 ctggcgggct tcaccacgga gcgcgaggac atcgtgcttt cggggcgcga aacgattttg
      961 cgcaatggcg cgttcgcggg ctatctcacc agcggcggct atggctacac gatcaggaaa
     1021 ccggtgggct tcggctatgt gagaaacgcc gacggggtgg acgaagccta tctcgaagcg
     1081 ggccgctacg agcttgtggt ggccaaggaa atcgtgaagg caaacataca cttgcagccg
     1141 atatatgaca gtttgaatca acgcgtgaaa tcatgaactg ggaccgtaaa gttcatatcc
     1201 tgatcatcgg cggcggggcc atcggcacgt cgctggccta tcacctgggg cgagacgggg
     1261 cgaaggacgt cctgctcgtc gaaaaggccc agctcaccga gggatgcacc tggcatgcgg
     1321 cgggccttgt ggggcagctt cgcggtaaga agaatctcac gcggctgatg cagaattcgg
     1381 tagcggtttt cgaccggctg gaagcggaaa ccgggcaggc catttcctgg aaaaaggtcg
     1441 gctcgctgcg ccttgcttcg agtgcggcgc ggtggagcga aatccagcgg tccatggggc
     1501 aggccaagag tttcggggtg gagtgccagt cgctgtcggc agcagaggcg aagactctg
//
LOCUS       DAORVN010000601         1557 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_95721_length_1557_cov_2.584554, whole genome shotgun sequence.
ACCESSION   DAORVN010000601 DAORVN010000000
VERSION     DAORVN010000601.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1557
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95721_length_1557_cov_2.584554"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..832)
                     /locus_tag="PKV23_10710"
     CDS             complement(<1..832)
                     /locus_tag="PKV23_10710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018699942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HQX85951.1"
                     /translation="MSDTGIGARVLRKEDKRFITGKGQYTDDINLKDQTYAYFVRSPH
                     AHATIKSINTAKAKKMPGVVDIFTSKEVEEDKIGGLICGWMIHSKDGSPMKAGPHPIL
                     ASGKVRFVGDQVAVVIAETREQAKAAAQAVAVSYGVLPHNVDAAQAMKTKAQVHDAAP
                     DNRVYNWSIGDQAATDAAFKTAAHVTKIDLINNRLIPNAMETRAAIGYYDTGTEHYTL
                     YTTSQNPHVARLVISAFHGLAPEHKLRVIPPDVGGGFGSKIFIYGEEVTCVWASKRIG
                     G"
     gene            complement(847..1311)
                     /locus_tag="PKV23_10715"
     CDS             complement(847..1311)
                     /locus_tag="PKV23_10715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014490495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HQX85952.1"
                     /translation="MPAISMKVNGKAVCGEVENRTLLVDFIRTNLGLTGTHVGCDTSQ
                     CGACVVHIDGKAVKSCTTLAVACEGADVTTIEGIAKNGKLHPMQQAFHENHGLQCGFC
                     TPGMIMASIDIVKRNGKKVSEEVIRRELSQNICRCTGYHNIVAAVQSAAEKM"
BASE COUNT          275 a          457 c          458 g          367 t
ORIGIN      
        1 ggccgccgat gcgcttggaa gcccagacac aggtgacttc ctcgccatag atgaagattt
       61 tcgaaccgaa gccgccgccc acatcgggag ggatcacccg cagcttgtgt tccggcgcca
      121 gcccatggaa cgccgagatg accaggcgcg ccacatgcgg attctggctt gttgtgtaaa
      181 gcgtatagtg ctcggtgccc gtgtcgtagt aaccgatggc ggcgcgggtt tccatggcgt
      241 tgggaatgag ccggttgttg atgagatcaa tcttggtcac atgcgccgcc gtcttgaagg
      301 cggcgtccgt tgccgcctga tcgccgatcg accagttgta aacccggttg tcgggcgcgg
      361 cgtcatggac ctgggccttg gttttcattg cctgggctgc gtccacgtta tgcggcagca
      421 cgccgtagga cacggcaacc gcctgggccg cggccttggc ctgttcgcgc gtttccgcga
      481 tgacaaccgc cacctggtcg ccaacaaagc ggaccttgcc gctggccaga atgggatgcg
      541 ggcccgcctt catcggcgag ccatccttgg aatggatcat ccagccgcag atcaatccgc
      601 cgatcttgtc ttcctcgact tccttggagg tgaagatatc gaccacgccc ggcatcttct
      661 tggccttggc ggtgttgatc gacttgatcg tggcatgggc atgggggctg cgcacgaaat
      721 aggcgtaggt ctggtctttc aggttgatgt cgtcggtata ctggcccttg ccggtgatga
      781 accgcttgtc ttccttgcgc agcacccgcg cgccaattcc tgtgtcggac atgttgctcg
      841 cctccgttac attttttcgg cagcggattg aaccgccgcc acgatgttat gatagcccgt
      901 gcagcggcaa atgttctgcg aaagctcgcg gcggatgact tcctcgctca ccttcttgcc
      961 attgcgcttc acgatgtcga tggaggccat gatcatgccg ggggtgcaga aaccgcactg
     1021 caggccgtga ttctcatgga aggcctgctg catgggatgg agcttgccgt tcttggctat
     1081 gccctcgatg gtggtgacgt cagccccttc gcaggcgacc gcaagcgtgg tgcaggattt
     1141 gaccgccttg ccgtcaatat ggaccacgca ggccccgcac tggctggtgt cgcagcccac
     1201 atgggttccg gtgagaccca gattcgtccg gataaaatcg accagcagcg tgcggttttc
     1261 aacctctccg cacaccgcct tgccattcac tttcatggaa attgcgggca ttcagcttac
     1321 ctccctggcg ttccctcgaa ggcattgaac ggccctcgtt ccgggaattc ttttctattc
     1381 tatctagatt ataatcatcc cagatttgca ccttggtcaa gccatggcaa ggggaatgaa
     1441 atgggcttgg ggtgcatgcg gctttttgca cgcgccgccg caatttccgg ctgcagcttg
     1501 gaccaataca gcagctttat ccaagaattc tggaaatctg ctttgatgat tggcacc
//
LOCUS       DAORVN010000602         1556 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_95785_length_1556_cov_3.814124, whole genome shotgun sequence.
ACCESSION   DAORVN010000602 DAORVN010000000
VERSION     DAORVN010000602.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95785_length_1556_cov_3.814124"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1220
                     /locus_tag="PKV23_10720"
     CDS             <1..1220
                     /locus_tag="PKV23_10720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012109670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HQX85953.1"
                     /translation="LWCTALGYGNEEIVEAAAQAMRKLSFTNVFGGKSHDGVIELSEK
                     LKEIAPCPTSKVLFGTSGSEANDMQMKLTWYYNNARGKPEKKKFISRMRGYHGVTIAS
                     GSLTGLPMVHTDFDLPIKNVLHTTCPHFYRGGQEGETEEQFTDRMAKDLDDLIQREGP
                     ETIAAFIAEPIMGAGGVVVPPMSYFPKINAVLEKYDVRFIADEVICGFGRTGNWFGSQ
                     TFGMKATSISMAKAITSAYFPMSAITIEEDLYQAMVDESKKIGTFGHGFTYTAHPVGC
                     AVALKTIEIYERDRIVEHVRKVAPVFKARLDKLAEHPLVGEARGVGLIGALELVADKK
                     TRRAFDPKKMVGATCVNFLQALGLINRAMGDSVAFCPPLIITEQEINEMFDMVGAALD
                     QTEAWVRKENLRQ"
     gene            complement(1214..>1556)
                     /locus_tag="PKV23_10725"
     CDS             complement(1214..>1556)
                     /locus_tag="PKV23_10725"
                     /inference="COORDINATES: protein motif:HMM:NF014997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HQX85954.1"
                     /translation="FLLDASQSWTLGLVTWLGAAAGIAMKFLFPGRFDRLAILIYLAL
                     GWVVLTAAGAFIQVLPPQVMSLIVAGGILYTAGIIFHLWEDLKYHNAIWHGFVAAAAA
                     CHFAAITMLYY"
BASE COUNT          345 a          415 c          514 g          282 t
ORIGIN      
        1 ggctgtggtg cacggcgctg ggttacggca atgaagagat cgtcgaggcg gcagcgcagg
       61 cgatgcgcaa gctatccttc accaatgtgt tcggcggcaa gtcgcatgac ggggtgatcg
      121 agctttcgga aaagctgaag gagatcgcgc cctgccccac ctcgaaggtg ctgttcggca
      181 cgtcgggctc ggaagccaat gacatgcaga tgaaactcac ctggtattac aataatgcca
      241 ggggaaagcc ggagaagaaa aagtttattt cgcggatgcg cggctatcat ggcgtcacca
      301 tcgcctcggg atcgctcacg ggccttccca tggtgcatac ggattttgat ctgccgatca
      361 agaatgtttt gcatacgacg tgcccgcatt tttaccgcgg cgggcaggag ggcgaaacgg
      421 aagagcagtt tactgaccgc atggcgaagg atctcgatga cctgatccag cgcgaggggc
      481 cggagacaat tgcggcgttc attgcggagc ccatcatggg ggccggcggc gtggtggtgc
      541 cgccgatgag ctatttcccc aagatcaatg cggtgctgga aaagtacgat gtgcgtttca
      601 tcgccgatga agtgatctgc ggttttggcc gcaccggcaa ctggttcggc tcgcaaacct
      661 tcgggatgaa agccacgtcg atctccatgg ccaaggccat cacctcggcc tatttcccga
      721 tgagcgccat caccatcgag gaagatctgt accaggcgat ggtggatgag agcaaaaaga
      781 tcggcacgtt cggccatggc ttcacttata cggcgcatcc ggtgggctgt gcggtggcgt
      841 tgaagaccat cgagatttat gagcgcgaca ggatcgtcga gcatgtgcgg aaggtggcgc
      901 cagtgttcaa ggcgcggctc gacaagctgg cggagcatcc gctggtgggc gaagcacgcg
      961 gcgtcggcct gatcggcgcc cttgaactgg tggccgacaa gaaaaccagg cgcgcctttg
     1021 acccgaagaa gatggtgggc gccacgtgcg tcaatttcct gcaggcgctt ggcctgatca
     1081 accgcgccat gggcgactca gttgccttct gcccgccgct catcatcacg gagcaagaaa
     1141 tcaacgagat gttcgacatg gtgggcgcgg cactggacca gaccgaagct tgggtgcgca
     1201 aggaaaacct gcgtcagtag tacagcatgg tgattgcggc gaagtggcag gcggcggcag
     1261 cggcgacaaa gccatgccag atggcattgt ggtatttcag atcctcccaa aggtggaaga
     1321 tgatgccggc ggtgtagaga atgccgccgg cgacaatcag gctcatgacc tgcggcggga
     1381 gaacctggat gaaggcgccc gctgcggtga gaacgaccca gcccagggcg agatatatta
     1441 ggatggcaag acggtcgaag cgtccgggga acagaaactt catcgcaatg ccggcggccg
     1501 cgcccagcca ggtgacaagc ccaagcgtcc agctttgcga cgcatcgagc aggaac
//
LOCUS       DAORVN010000603         1556 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_95798_length_1556_cov_3.427715, whole genome shotgun sequence.
ACCESSION   DAORVN010000603 DAORVN010000000
VERSION     DAORVN010000603.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95798_length_1556_cov_3.427715"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..657)
                     /locus_tag="PKV23_10730"
     CDS             complement(<1..657)
                     /locus_tag="PKV23_10730"
                     /EC_number="2.1.3.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012493901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HQX85955.1"
                     /translation="MNWIKNFVRPKIRSILGVKRDVPENMWIKDPDSGQMVFYRDLEA
                     NQFVFPGSDHHHRLAPDLRLKSMFDDGVYETLEFPAVPSDPLKFRDERKYVDRLKEAR
                     QKANSEDAIHAGFGTLDQLPIVIAVQDMGFIAGSLGTAAGEAIITAMRFAVTKKCPFV
                     FFVASGGARMQEGILSLMQMPRTTVMVQELRAHKLPYIVVLTNPTTGGVTASYAMLGD
                     F"
     gene            complement(663..1481)
                     /gene="trpA"
                     /locus_tag="PKV23_10735"
     CDS             complement(663..1481)
                     /gene="trpA"
                     /locus_tag="PKV23_10735"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014438732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HQX85956.1"
                     /translation="MTGRIERRFAELKQKGRAALVTFVTAGDPDYETSLKIIKGLPKA
                     GADIIEFGMPFTDPMADGPAIQAAGLRALHAGQTMKKNLKLVREFRAGDKETPVVLMG
                     YYNPIYVYGVDRFLRDAKSAGVDGLIVVDLPPEEDDELCLPAIAADINFIRLATPTTD
                     DQRAPTVFRNTSGFVYYVSVLGITGTKAPDLKSVEKNVNRLRKHTNLPIAVGFGVKTA
                     AQARAIARSADGVVVGSALVSAIKDSLTKAGKPSPKTVKAVHSLVSDIARGVRS"
BASE COUNT          309 a          449 c          478 g          320 t
ORIGIN      
        1 gaaatcaccc agcatggcat aggaagcggt aacgccgccg gttgtcggat tggtgagaac
       61 gacgatgtag ggcagtttat gggcgcgcag ttcctgcacc atgaccgtcg tgcgcggcat
      121 ctgcatcaat gacaggatgc cttcctgcat gcgggccccg ccggaggcca caaagaacac
      181 gaaggggcat ttcttggtaa ccgcaaaacg catggcggtg atgatggctt cgccggcggc
      241 tgttcccagc gagcccgcga tgaagcccat gtcctgcacc gcgatcacga tgggaagctg
      301 gtccagcgtg ccgaagcccg catgaatggc gtcctcgctg ttggccttct ggcgcgcctc
      361 cttcaggcgg tcgacatatt tgcgctcgtc cctgaatttc agcggatcag acggcaccgc
      421 gggaaattcc agggtttcat agacgccgtc atcgaacatg gatttcagcc gcaggtccgg
      481 cgccaggcgg tgatgatggt cggagcccgg gaaaacaaac tggttggcct cgaggtcgcg
      541 gtagaaaacc atctggccgg aatcgggatc cttgatccac atgttctccg gcacgtcgcg
      601 cttgacgccg agaatggagc ggattttcgg ccggacgaaa ttcttgatcc agttcacctg
      661 atctagctcc tcacgccacg ggcaatgtca ctcaccagcg agtgtaccgc ttttacggtt
      721 ttcggtgaag gctttccggc ctttgtgaga ctgtccttaa tggcgctgac aagggccgag
      781 cccacaacga cgccatcggc acttctggca atggcgcggg cctgggccgc ggtcttcacg
      841 ccgaagccca cggcaatcgg cagattcgtg tgcttgcgca ggcggttcac attcttttcc
      901 acgctcttga gatcgggcgc cttggtgccg gtgatgccga gcaccgaaac ataatagaca
      961 aaaccactgg tattcctgaa cacggtgggg gcgcgctggt catcggtggt gggcgttgcc
     1021 aggcggatga aattgatatc ggcggcgata gctggaagac agagttcgtc atcttcctcg
     1081 ggcgggaggt cgacaacgat caggccgtcg acgccggcgg acttggcatc cctgaggaac
     1141 cgatcgacgc catagacata aatgggattg tagtaaccca tcagcaccac tggcgtttcc
     1201 ttgtcgcccg cgcggaattc gcgcaccagc ttgaggtttt tcttcatggt ctggcctgca
     1261 tgcaaggcac gcaggcccgc cgcctggatg gcggggccat cggccatggg atcggtgaaa
     1321 ggcatgccga actcgatgat gtctgcacca gccttgggca ggcccttgat gatcttgaga
     1381 gaggtttcgt agtcgggatc gccggcggtc acgaaagtca caagtgctgc gcggcccttt
     1441 tgtttcagct cggcgaaacg gcgttcgatg cggcccgtca caatttcacc ccgagaatgt
     1501 ccgcaaccgt gaaaatatcc ttgtcgccgc ggccgcacat gttcatcacc atcaag
//
LOCUS       DAORVN010000604         1556 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_95810_length_1556_cov_2.954697, whole genome shotgun sequence.
ACCESSION   DAORVN010000604 DAORVN010000000
VERSION     DAORVN010000604.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95810_length_1556_cov_2.954697"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..469)
                     /locus_tag="PKV23_10740"
     CDS             complement(<1..469)
                     /locus_tag="PKV23_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HQX85957.1"
                     /translation="MLMVLPVRALDVEISNFKLANGMEVVVIPNNRAPVVTHMVWYRV
                     GSADEVSGKSGLAHFLEHLMFKGTSKRAPGEFDRLIDINGGEGNAFTTSDYTAYFQRI
                     ASDRLELMMELEADRMQNLVLTDENVLPELDVVREERRERTDNDPSALLGEQID"
     gene            complement(597..1067)
                     /locus_tag="PKV23_10745"
     CDS             complement(597..1067)
                     /locus_tag="PKV23_10745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85958.1"
                     /translation="MKTVWKISGVLAMSLVLAGCGGASRLLGGMGLGSSDPAPQAPVV
                     RTGNNLALPPDLQLRPPGTATETYQPTPPPPAPVETASLDEGLYAPVAPAPAPAVKKD
                     IYAEYGISKTKPDGTPKLEGDLRAELKQAILKRKRETDPNYGTVGNIGNIFKDG"
     gene            complement(1117..>1556)
                     /gene="lspA"
                     /locus_tag="PKV23_10750"
     CDS             complement(1117..>1556)
                     /gene="lspA"
                     /locus_tag="PKV23_10750"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020180995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HQX85959.1"
                     /translation="AVDQTHKWWMLNIYDIGARGRVSFTPFLDLLLVWNKGISYGLFN
                     NNAQGILVGLSLLVSAMLWIWLCRSHRPVTAAALGLVIGGALGNALDRLIRGAVADFF
                     QLHWGNWTWYVFNLADVAIVAGVALLLYESFRERNGAAGLGNA"
BASE COUNT          306 a          449 c          489 g          312 t
ORIGIN      
        1 catcgatctg ttcacccagc agcgcggaag gatcattgtc ggtgcgctcg cggcgctctt
       61 cgcgcacgac atcgagttcg ggaaggacat tctcatcggt gagaacgaga ttctgcatgc
      121 ggtctgcttc cagctccatc atcagttcca gccggtcgct ggcaatgcgc tggaagtagg
      181 cggtgtagtc gcttgtggtg aaggcattgc cctcgccgcc attgatgtcg atcaggcggt
      241 cgaactcgcc cggggcgcgt ttgctggttc ccttgaacat caggtgttcg agaaaatgcg
      301 ccaggccgga tttgcccgag acttcatcgg cggagcccac gcggtaccac accatgtggg
      361 tgacgacggg agcgcggttg ttgggaatga cgacgacttc catgccgttg gcaagcttga
      421 agtttgaaat ttccacatcg agcgcgcgga ccggcagaac catgagcatg atcagcgcaa
      481 gctgcaggac ggctatcttg acggcgcgca tgaatcaact ttcccggtca gaaatttgtg
      541 gccggaagat gcaggagaag gccctgcccg tccaatgaga tttttgtaat cttcgactag
      601 ccgtctttaa agatgttgcc gatattgccg acggtgccgt agttcgggtc agtctcgcgc
      661 ttgcgcttga gaatggcctg cttcagttcg gccctcaggt cgccttcaag cttaggcgtg
      721 ccatcgggct tggttttcga aataccgtat tcggcataga tatctttctt gaccgccggt
      781 gcgggcgcgg gcgccacggg tgcgtaaagc ccctcgtcga ggctggcggt ttcaaccggt
      841 gctggcggcg ggggagttgg ctggtaggtt tccgttgcgg tgcccggcgg gcgcaactgg
      901 aggtctggcg gcagggctag attgttgccg gtccggacaa cgggggcctg cggggcgggg
      961 tcagagcttc caaggcccat gccgcccagc agccggcttg cgccgccaca accggccaga
     1021 accaggctca tggccaaaac cccggatatt ttccagactg ttttcatgcc tgttactccc
     1081 acctgccgtc cttttgggcg cggtttgcga tctgaatcag gcatttccaa gacctgccgc
     1141 cccattccgt tcccggaagg actcatagag caagagggca acccccgcaa cgatggccac
     1201 gtcagcaagg ttaaagacgt accaggtcca gtttccccag tggagctgga aaaaatcggc
     1261 aaccgccccc cggatcaggc ggtccagggc attgccgagg gcgccgccaa tgaccagccc
     1321 aagggcagcc gcggtgaccg gccgatggga ccggcacagc cagatccaca gcatggcgga
     1381 gacaagcaga ctcaggccca ccagaatgcc ctgggcattg ttgttgaaca ggccgtagga
     1441 aatgcccttg ttccagacga ggaggagatc gagaaatggc gtgaaagaca cgcggccccg
     1501 ggcgccgatg tcataaatat tcagcatcca ccacttgtgg gtctggtcga ccgcga
//
LOCUS       DAORVN010000605         1552 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_96286_length_1552_cov_2.936540, whole genome shotgun sequence.
ACCESSION   DAORVN010000605 DAORVN010000000
VERSION     DAORVN010000605.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1552
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96286_length_1552_cov_2.936540"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..588)
                     /locus_tag="PKV23_10755"
     CDS             complement(<1..588)
                     /locus_tag="PKV23_10755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007822296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HQX85960.1"
                     /translation="MTLITEIDYGTPKSKATTMVTLEIDGQSITVPEGTSIMRAAMEL
                     GTQIPKLCATDNMASFGSCRLCLVEIEGRKGTPASCTTPVAPGIKVHTQTDRLGKLRQ
                     GVMELYLSDHPLTGDNAMQDMADTVGLANVRYGFEGDNHVSPVRADGEANPRFKEKDE
                     SNPYFAFDPAKCIVCSRCVRACEEVQGTFALTIDGR"
     gene            complement(600..1552)
                     /locus_tag="PKV23_10760"
                     /pseudo
     CDS             complement(600..1552)
                     /locus_tag="PKV23_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496686.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
BASE COUNT          278 a          484 c          481 g          309 t
ORIGIN      
        1 gcggccatca atggtcagcg cgaaggtgcc ttgcacttcc tcgcaggcgc gcacgcagcg
       61 cgagcagaca atgcatttcg ccggatcgaa agcgaaatag ggattgcttt catccttttc
      121 cttgaagcgg ggattggctt cgccgtccgc ccgcaccggt gacacgtgat tgtcgccctc
      181 gaagccatag cgcacattgg caaggcctac cgtgtccgcc atgtcctgca tggcgttgtc
      241 gccggtcagc gggtggtcgg aaaggtagag ttccatgacc ccctggcgca gcttgccgag
      301 ccggtccgtc tgggtgtgga ccttgatgcc gggggcaacc ggcgtggtgc acgaggcggg
      361 cgtgcccttg cggccttcga tttcaaccag gcacaggcgg catgagccga agctggccat
      421 gttgtcggtg gcgcagagct ttggaatctg cgtgccaagc tccatggcgg cgcgcatgat
      481 ggaggtgcct tccggcaccg tgatggactg gccatcgatt tccagcgtca ccatggtggt
      541 ggctttggat ttcggcgtgc cgtagtcgat ttctgtgatc agggtcatgg gtagctacct
      601 cattccgccg cctgcatttt agcaggccgg tcaaaatctt caggaaaatg tttcacggcg
      661 ctttgcaccg gcatgggggt caaacctccc atggcgcaga gcgagccatc cgtcatcgtc
      721 tggcaaaggt catcaagcag cagcaggttc tcttcgcgcg ctacgcccgc cacgatcttg
      781 tcaatcactt ccatgccccg ggtggagcca atgcggcagg gcgtgcactt gccgcaggat
      841 tcaatggcgc agaactccat ggcaaatcgc gcctgcgccg ccatgtcgac gctgtcatca
      901 aacaccacca cgccgccatg gcccaccatc gcgccattgg ccgcaaaggc ttcatagtcc
      961 atttccaggt tgagttgcga agcgggcagg taagccccca gcgggccgcc gacctgcacg
     1021 gcgcgcaagg gtttccccgt tgccgtgccg ccgccgaaat cattgatgag ctggcccagc
     1081 gtgatgccga aggctttttc gacaaggcca ccctgcctga tattgccggc gagctggaag
     1141 ggcagcgtgc cgcgcgaacg gcccatgccg taattctcgt aagccttgcc gccttccgcc
     1201 agaatgtagg gcacggcggc aaagctcagc acgttgttga tgacggtggg cttgccgaac
     1261 aggccataaa gcgccgggat cggcggcttg ggccgcacca tgccgcgctt gccttccagg
     1321 ctctcgagca tggaggtttc ttcgccgcag atgtaggcgc cggccccgcg ccgcacgtcg
     1381 agcgcgaagg aatgcactga accctgaata ttccgcccca gccaattgcc tgttgtcgcg
     1441 atgcggatgg catccttcat cgcgcggatc gcatccggat attccgagcg gatatagatg
     1501 aagccttagc tggcgcccac ggcgacgccg gcgattgtca tgccttcgat ca
//
LOCUS       DAORVN010000606         1549 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_96700_length_1549_cov_2.240964, whole genome shotgun sequence.
ACCESSION   DAORVN010000606 DAORVN010000000
VERSION     DAORVN010000606.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96700_length_1549_cov_2.240964"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            220..852
                     /locus_tag="PKV23_10765"
     CDS             220..852
                     /locus_tag="PKV23_10765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012537444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HQX85961.1"
                     /translation="MQILQRRTHMNMPVDNAALDALIRQRLDEILPQKLEEIRAAKTP
                     SMTIIATKGTLDWAYPPFILGSTAAALGWDVSIFCTFYGLNLLKKDISDLKVSPLGNP
                     GMPMKMPFGPDWFKSLNWNIPNLIQAGIPGFETMATMLMKQTIKNNGVAEIGELRGLC
                     LEAGVEMIGCQMTVDLFGFKHDDFIPEVKEYCGAATFLPMAQQADVSLYM"
     gene            890..>1549
                     /locus_tag="PKV23_10770"
     CDS             890..>1549
                     /locus_tag="PKV23_10770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85962.1"
                     /translation="MKFRIKITAATLAMTLAPAAWATDGYFSHGYGMKAKGMGGAATA
                     MASDTFGGANNPASMVWVGDRLDVGVDLFSPRRSAERTGSPAGINTGGNVDSGSNLFA
                     VPEFGYNKMLGWNMSLGVTVYGNGGMNTDYPGGQISAGAPGVVDTVCDGFNGTAVASG
                     ATSYNMLCGTGRLGIDLMQLVIAPTFAMKINKDHSLGVSPLIGYQRFKAKGLDGFQGF
                     TP"
BASE COUNT          353 a          447 c          444 g          305 t
ORIGIN      
        1 aagggcaagg aatacgagtt tttcatcaag cgcaaataac aaacctcccg acgcggcagc
       61 cggtgatccg gccgccgcct cttctgcgcc aattcttgcg gcgcaacata tgtccttgat
      121 attggccgat aaataaattc atctggctga tagaggaact ttagaacatc attattcgtt
      181 aataccataa ataaagacaa ggtcggcctc tacgcaacct tgcagatcct tcaaaggagg
      241 acgcacatga acatgccagt agacaacgcc gccctggatg cgctgatccg acaacgcctc
      301 gacgagattc ttccgcagaa actcgaggaa atcagggctg ccaaaacacc ctcgatgacc
      361 atcatcgcca ccaagggtac cctcgactgg gcttacccgc ccttcatcct cggctcgacc
      421 gccgccgccc tgggctggga cgtctccatc ttctgcacct tctacggcct gaatctgctg
      481 aagaaggaca tcagcgacct gaaagtgagc ccgctgggca atcccggcat gccgatgaaa
      541 atgcccttcg gtccggactg gttcaagagc ctcaactgga acatcccgaa cctcattcaa
      601 gccggcatcc ccggcttcga gacgatggcg acgatgctca tgaagcagac catcaagaac
      661 aacggcgttg ccgagatcgg cgaattgcgt ggtctgtgcc ttgaggccgg cgtggaaatg
      721 atcggctgcc agatgacggt cgacctgttc ggcttcaagc acgacgattt cattccggaa
      781 gtgaaggaat actgcggcgc ggcgacgttc ctgccaatgg cgcagcaggc cgatgtcagt
      841 ttgtacatgt aagcggcaag cattcacgca aaccaaggag gggagaaaca tgaagttcag
      901 gataaaaatc accgcagcga cgttggccat gaccctggcg cccgccgcct gggcgaccga
      961 cggctatttc tcgcatggct atggcatgaa agccaagggt atgggcggcg ccgcaacggc
     1021 gatggcctcc gatacctttg gcggcgccaa taacccggcc agcatggtct gggtgggcga
     1081 tcgactggat gtcggtgttg acctgttcag cccgaggcgc agtgcggagc gtaccggcag
     1141 ccctgctgga atcaataccg gcgggaacgt ggatagcggc agcaatctct tcgccgttcc
     1201 ggagttcggc tacaacaaga tgttgggctg gaacatgtcc ctcggcgtca ccgtttatgg
     1261 caatggcggc atgaatacgg attacccggg cggacagatt tcagcaggtg cgccgggcgt
     1321 cgttgatacc gtgtgcgacg gcttcaacgg aaccgcggtt gcgtcgggtg cgacttcata
     1381 caatatgctg tgcggcacgg gcagacttgg catcgacctg atgcagctgg tcattgcgcc
     1441 gacctttgcc atgaagatca acaaggatca ctccttgggc gtttctccgc tgatcggcta
     1501 tcagcgcttc aaggccaagg gccttgacgg gttccagggg ttcacgcca
//
LOCUS       DAORVN010000607         1546 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_96988_length_1546_cov_3.040912, whole genome shotgun sequence.
ACCESSION   DAORVN010000607 DAORVN010000000
VERSION     DAORVN010000607.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1546
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96988_length_1546_cov_3.040912"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..819)
                     /locus_tag="PKV23_10775"
     CDS             complement(<1..819)
                     /locus_tag="PKV23_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018324423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQX85963.1"
                     /translation="MSIRLKFCGAARTVTGSCYLLQTDEAKVLIDCGMFQGSRTEREL
                     NWRNFPFDPASLDAMLLTHGHIDHIGLIPKLVKNGFNKKIHCTAATVDLCAVMLPDSG
                     FIQESDAKFQNKRNRQRGLPEIEPIYTLEDAMNALEHFAPLEYETWIVPAKGIRARWW
                     NAGHLLGSASIEVEVSRAGKPPTRILFSGDIGPDFKMLHPDPEAPTGFDYVICESTYG
                     GKDRFELDLTGGWGGVEGKLWQAKGWVLPRTREGELALKAERFELGRLAPLVAGS"
     gene            complement(819..>1546)
                     /locus_tag="PKV23_10780"
     CDS             complement(819..>1546)
                     /locus_tag="PKV23_10780"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HQX85964.1"
                     /translation="AAPAYRLPALDQEFLLGDSTRGMRFHLEYQKAEEHLRAWGVEST
                     VVVFGTARCRENDGSGWYEGARKFAEICSLHGGAKLDHEVTRHNVIATGGGPGIMEAA
                     NRGANDAGAPSIGYNITLPREQEPNPYSTPDLTFRFHYFAMRKMHFAMRAAALVVFPG
                     GFGTLDELFEILTLRQTGKMTKPLPVILYDSKFWNTAINFEILLKSGLIHPDDFNLFG
                     FADTPDQAWRELVVRGLNIPQEQ"
BASE COUNT          306 a          430 c          511 g          299 t
ORIGIN      
        1 cgagcccgcc accagcggcg ccagccgacc cagctcgaag cgctccgcct tgagggccag
       61 ctcgccctcg cgcgtgcgcg gcaacaccca gcccttggct tgccacagct tgccctcgac
      121 gccgccccag cccccggtca ggtccagctc gaagcggtcc ttgccgccat aggtggattc
      181 gcagatgaca tagtcaaagc cggttggcgc ttccgggtcg ggatgcagca tcttgaaatc
      241 ggggccgatg tcgccggaga aaaggatgcg ggtgggcggc ttgccagccc ggctgacctc
      301 aacttcaatt gaagcgctcc ccagaagatg gccggcgttc caccagcggg cgcgaatgcc
      361 cttggcgggc acaatccagg tttcatattc gagcggcgca aaatgctcga gggcattcat
      421 ggcgtcttcc agcgtgtaga tgggctcaat ctcgggaagg ccgcgctgac ggttgcgctt
      481 gttctggaac ttggcatccg attcctggat gaagccggag tcgggcagca tgacggcgca
      541 gagatcgacg gtggccgcgg tgcaatggat tttcttgttg aaaccgttct tcacaagttt
      601 tggaataagc ccgatgtgat caatgtggcc atgggtaagc agcatggcat cgaggcttgc
      661 cggatcaaac gggaaattgc gccagttcag ttcgcgctcg gttctggacc cctggaacat
      721 gccgcaatcg atgagaacct ttgcctcatc ggtttgcagg agatagcagg agccggtgac
      781 ggtccgcgcg gcaccgcaga atttgagtcg gatggacatc attgctcctg gggaatgtta
      841 aggccgcgaa cgacgagttc gcgccaggcc tggtctggcg tgtcggcaaa gccgaagagg
      901 ttgaaatcgt ctggatggat cagaccagac ttcagcagaa tttcgaaatt gatggcggtg
      961 ttccagaatt tcgaatcata aaggatgacg ggcagcggct tagtcatctt gccggtttgc
     1021 ctgagggtga gaatctcgaa aagttcatcg agcgtgccga agccgccggg gaacacgacg
     1081 agggcggcag cccgcatggc gaaatgcatc ttgcgcattg cgaaataatg gaaacggaag
     1141 gtgaggtccg gggtcgagta gggattcggc tcctgttcac ggggcagcgt gatgttgtag
     1201 ccgatggagg gcgcgccagc gtcattggcg ccgcggttgg ctgcttccat gatgcccgga
     1261 ccgccgccgg tcgcgatgac attgtggcgg gttacctcgt gatcgagttt ggcgccgccg
     1321 tggagcgaac agatttcggc gaatttgcgc gccccttcat accagccgga gccgtcattt
     1381 tcccggcagc gggcggtgcc gaaaaccacc acggtggatt caacccccca ggcgcgcagg
     1441 tgttcctcgg ccttctggta ctccagatgg aagcgcatgc cgcgggttga atcaccgagg
     1501 aggaattcct ggtcaagggc gggcaggcga taggcagggg cagcaa
//
LOCUS       DAORVN010000608         1546 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_97032_length_1546_cov_2.177062, whole genome shotgun sequence.
ACCESSION   DAORVN010000608 DAORVN010000000
VERSION     DAORVN010000608.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1546
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97032_length_1546_cov_2.177062"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="PKV23_10785"
     CDS             complement(<1..184)
                     /locus_tag="PKV23_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006725726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HQX85965.1"
                     /translation="MRILIVTDAWPPQVNGVVRTLQTVGRELGKRGHEVRFITPEGHR
                     TWAIPSYAEIRLSLVGS"
     gene            352..1257
                     /locus_tag="PKV23_10790"
     CDS             352..1257
                     /locus_tag="PKV23_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016843343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HQX85966.1"
                     /translation="MKDPYDILGVARTATSDDIQKAYRRLAKKLHPDLNPGDKKAEER
                     FKELSIANDILSDENKRKKFDRGEIDALGAETPRQQYYKDYASRAGAEHAYQNPSGFA
                     DFAGADDIFAELFRRQAQQARRAKGADAHYRLSVEFLDAITGATRRLTLPDGGTLDVT
                     IPPGIQEGQTLRLRGKGMPSAGEGEAGDALVEVSIRPHRFFTRVDDDIHVELPVTLAE
                     AFLGASIKVPTPSGSVMLKVPKGSNTGTVLRLKGKGVARRAGAGDELVRLKVVLPTGP
                     DPELETFLNKWKPRDGYQPRKEMQP"
     gene            1254..1523
                     /locus_tag="PKV23_10795"
     CDS             1254..1523
                     /locus_tag="PKV23_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012754147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HQX85967.1"
                     /translation="MMLTKLEFLSRARLDQETLEVCMSEAWLLPSETAGEPAFTEIDL
                     ARAKLIRDLKHDMGVNNEGIGIILDLLDQMHSLRKAMAETLPVKR"
BASE COUNT          343 a          455 c          453 g          295 t
ORIGIN      
        1 ccgatccgac gagactgagc ctgatttccg cataggaggg gattgcccag gtccggtggc
       61 cttccggcgt gatgaagcga acctcatggc cgcgtttgcc gagttcgcgg cccacggtct
      121 ggagggtgcg gacaacgccg ttaacctgcg gtggccatgc atctgtcaca atcaggattc
      181 gcatcgtccc tcctagagtt gctttgggtc cccctcaggc atgaacatca caattgcatg
      241 acagcctgga ctgccttgtc atgctcctga aggcggaacg ccgcatttgc aatggcgagt
      301 gcagtccctt atcaattggg cataaagaac attttgctga acggagcgaa agtgaaagac
      361 ccatatgaca ttctgggggt cgcgcgcacc gccacctctg atgacatcca gaaggcttac
      421 cgccgcctgg caaaaaagct gcatcccgat ctcaaccccg gagacaaaaa ggcggaagag
      481 cgcttcaagg aactctccat agccaacgac atattgtcgg atgagaacaa gcgcaaaaaa
      541 tttgatcgcg gtgaaatcga cgcgctgggc gccgaaacgc cgcgccagca atattacaag
      601 gactatgcct cacgggctgg cgccgaacat gcttaccaga acccttcggg ctttgccgat
      661 tttgccggtg ccgacgatat tttcgcggaa ctgtttcggc gccaggcgca gcaggcccgg
      721 cgtgccaaag gcgcggacgc gcactaccgg ctttccgtcg agtttctcga tgccatcaca
      781 ggggcaaccc ggcgtttgac cctgcccgat ggcgggacac tggacgttac aattccgccc
      841 ggtattcaag aggggcagac cctgcgtctc cggggaaaag gaatgccgtc tgccggcgag
      901 ggcgaagcgg gcgatgccct ggtggaagtc tcaatccgtc cccatcgctt ctttacgcgg
      961 gttgacgatg acattcacgt tgaactgccg gtcactctgg cggaagcatt tctgggcgcc
     1021 agcatcaagg tgccgacgcc gtccggctcc gttatgctca aagtgccgaa aggctcaaac
     1081 acgggtacag tcctgcgcct taagggcaaa ggcgtagcgc ggcgcgccgg cgcaggcgat
     1141 gaactcgtga ggctcaaggt cgtgttgcca accgggccgg atcccgaact tgaaacgttt
     1201 ctcaacaaat ggaagccccg cgatggttat cagccgcgaa aggagatgca gccatgatgc
     1261 tcaccaagct cgagttcctt tcccgcgccc gccttgatca ggaaaccctt gaggtgtgca
     1321 tgagtgaagc ctggctgctt cccagtgaga ccgcaggcga accggcattc accgagatcg
     1381 atctggcgcg cgctaaattg atccgcgatc tgaagcatga catgggcgtc aacaatgagg
     1441 gaataggcat aatcctcgac ctcctcgatc agatgcacag tttgcggaag gccatggctg
     1501 agacccttcc agtcaagcgc tgatggcacc gaactgggca ggccct
//
LOCUS       DAORVN010000609         1539 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_97808_length_1539_cov_1.727089, whole genome shotgun sequence.
ACCESSION   DAORVN010000609 DAORVN010000000
VERSION     DAORVN010000609.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1539
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97808_length_1539_cov_1.727089"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..212)
                     /locus_tag="PKV23_10800"
     CDS             complement(<1..212)
                     /locus_tag="PKV23_10800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85968.1"
                     /translation="MEVNAGSNAGGEVAAAVKSYWLGALLHGLEGEEFSAFGTAKEDG
                     GVHLVEVPAGSAALAGTTYPLDRERVA"
     gene            complement(223..993)
                     /locus_tag="PKV23_10805"
     CDS             complement(223..993)
                     /locus_tag="PKV23_10805"
                     /EC_number="1.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008872940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-iditol 2-dehydrogenase"
                     /protein_id="HQX85969.1"
                     /translation="MKLQGKIAVITGGARGIGRAIAESYAKEGARVVIADLLLDEAKE
                     TARAIGPDALAVAVDVSKRSSIADMARFVAEKAGPANILVNGAAIFNMAPLSEISEEH
                     FDRQFDINVRGLLFTTQAIAAQMVAAGKGGKIINFSSQAGRRGEPRVAVYCASKAAVI
                     SLTQSLAHELIKQRINVNAIAPGVIDTPMWDQVDALFAKYENRPLGEKKRLVGEAVPY
                     GRMGVPGDITGAAVFLASADSDYIVGQTLNVDGGNYMS"
     gene            complement(1006..>1539)
                     /locus_tag="PKV23_10810"
     CDS             complement(1006..>1539)
                     /locus_tag="PKV23_10810"
                     /inference="COORDINATES: protein motif:HMM:NF025397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-TA family PLP-dependent enzyme"
                     /protein_id="HQX85970.1"
                     /translation="ADADKFLARALELSQQAGLKTDVVSSGGSPDMWKDEGLAHVTEY
                     RVGTYIYFDRSLAERGVCTYDECAVSVLATVVSRPNDQRALIDAGSKALTSDLLGLKG
                     YGVVRDLGFAKIYNVNEEHGYLDIAGCNDKPKVGDLVRVIPNHVCPVINLFDKVVLVR
                     GREVLGAVKVDARGKVQ"
BASE COUNT          274 a          490 c          465 g          310 t
ORIGIN      
        1 gccacgcgtt cccggtcgag cgggtaggtg gttccggcca gggcggcgct gccggcgggg
       61 acttcaacca ggtgtacgcc gccgtcttcc ttggcggtgc cgaaggcaga gaattcctcg
      121 ccctccagcc cgtgcaggag cgcgccgagc cagtacgatt tgaccgcagc cgcaacctcc
      181 cctcccgcgt tcgacccagc gtttacctcc acggagggaa tttcagctca tgtagtttcc
      241 gccgtcgacg ttgagggtct ggcccacaat gtagtcgcta tcggcactcg cgaggaacac
      301 ggcggctccc gtgatatcgc ccggcacgcc catgcggccg tagggcaccg cttcgccgac
      361 caggcgtttc ttttcgccga gcgggcggtt ctcatatttg gcaaacaggg cgtcaacctg
      421 gtcccacatg ggggtgtcga tcacgccggg tgcgatggca ttgacattga tgcgctgctt
      481 gatcagttcg tgggccaggc tctgggtcag gctgatgact gccgccttgg aggcgcagta
      541 gacggcaacg cgcggttcgc cgcgccggcc cgcctggctt gagaagttga tgatctttcc
      601 gccctttccg gccgccacca tctgtgcggc gatggcttgg gtggtgaaca gcaggcccct
      661 gacgttgatg tcaaactgcc ggtcgaaatg ttcctcggaa atttccgaaa gcggcgccat
      721 gttgaagatg gcagcgccat tcaccaggat gtttgccggc cccgcctttt ccgccacgaa
      781 gcgcgccatg tcggcgatgg aactgcgctt gctcacatcg actgcaacgg ccagcgcatc
      841 agggccgatg gccctggcag tctcctttgc ctcatcaagc agcaggtcgg caatgacgac
      901 gcgggcccct tccttcgcat aactttccgc aatggcgcgg ccgatgccgc gcgccccgcc
      961 ggttatcact gcgatctttc cctgaagctt catctgattt tccccttatt ggaccttgcc
     1021 gcgggcatcg actttcacgg cgccaagaac ttccctgccg cgcaccagca cgaccttgtc
     1081 gaaaagattg atgaccgggc agacatggtt cggaatcacg cgaacgagat cgccgacttt
     1141 cggtttgtca ttgcagccgg cgatgtcaag atagccatgc tcctcattca cgttatagat
     1201 cttggcaaag ccgagatcgc ggacaacacc atagcccttg aggccgagca ggtcgctggt
     1261 gagcgccttg gaacccgcat cgatcagggc gcgctggtca ttggggcggc tcacaacggt
     1321 ggccagaacg ctcaccgcgc attcgtcata ggtgcagaca ccgcgctcgg ccaatgaacg
     1381 gtcaaaataa atgtaagtgc cgacgcgata ttcagtgacg tgggcaaggc cctcatcctt
     1441 ccacatgtcg ggagagccgc cggagcttac cacgtcggtc ttgagccccg cctgctggga
     1501 aagctccagc gctctggcga ggaatttgtc ggcgtcggc
//
LOCUS       DAORVN010000610         1536 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_98076_length_1536_cov_3.916948, whole genome shotgun sequence.
ACCESSION   DAORVN010000610 DAORVN010000000
VERSION     DAORVN010000610.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98076_length_1536_cov_3.916948"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..600
                     /locus_tag="PKV23_10815"
     CDS             <1..600
                     /locus_tag="PKV23_10815"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQX85971.1"
                     /translation="AACPFSRPAHWLLGTVGRMQAVKDQPTLVRAFVRALEIDPTLKQ
                     RLRLVLIGDGPLRAECQQLLATANVAELAWLPGERSDIPELMRGLDCFVLPSLAEGIS
                     NTILEAMASGLPVIATNVGGNADLVTAGITGAIVPSADPEAMAQEIVHLATQSDTAKS
                     MGLAGRSTIEQKFSMEAMVATYQGAYDKLLHRSGANSKH"
     gene            612..>1536
                     /gene="asnB"
                     /locus_tag="PKV23_10820"
     CDS             612..>1536
                     /gene="asnB"
                     /locus_tag="PKV23_10820"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HQX85972.1"
                     /translation="MCGITGIFETRGKREIDRAVLHRMNESQFHRGPDEGGLHVEPGV
                     GLGHRRLSIIDLSTGQQPLYNEDQSVCVVFNGEIYNYQTLIPELQALGHTFHTRSDTE
                     VIVHAWESWGEKCVDRFRGMFAFALWDRNRETLFLARDRLGVKPLYYALLDDGTFLFG
                     SELKSILAHGGLKREIDPCAVEEYFAFGYVAEPRAIFKQAKKLPPAHTLLLRRGQPVG
                     EPREYWDVHFTLDNPISDADACAELTHRLEESIRLRMISEVPLGAFLSGGVDSSAVVA
                     MMAGLSNAAVNTCSIGFSDPAFNESEFAKIVA"
BASE COUNT          324 a          542 c          377 g          293 t
ORIGIN      
        1 gccgcttgcc ccttctctcg cccggctcac tggctgcttg gcaccgtagg ccgcatgcag
       61 gcagtcaagg atcaaccgac gctggtccgc gctttcgttc gagccctgga aatcgacccg
      121 acgctcaaac aacgactacg cctggtcctg attggcgacg gcccattgcg tgccgaatgc
      181 caacaattac tagctaccgc aaacgttgct gaactcgctt ggctacccgg tgaacgcagt
      241 gacatccctg agctcatgcg cgggctggat tgcttcgttc taccctcgct ggctgaaggc
      301 atatccaaca ccattctcga agccatggcc tctggcctgc ctgttatcgc caccaacgtc
      361 ggcggcaatg ccgacctcgt caccgcaggc attaccgggg caatcgtgcc gtcggctgac
      421 ccggaagcca tggctcagga aatcgtccac ctcgccaccc agtcggacac tgcaaaaagt
      481 atgggcctcg ccggccgcag caccatcgaa caaaaattca gcatggaagc catggtcgca
      541 acctatcagg gcgcctacga caaacttctg caccgctcag gcgcaaactc caagcactaa
      601 caggaaaaac catgtgtggc ataaccggca tttttgaaac ccgaggcaaa cgcgagatcg
      661 atcgtgcagt gctccaccgc atgaacgaat cgcaattcca ccgcggcccg gacgaaggcg
      721 gcctgcatgt cgaacccggt gtcgggcttg gccaccgccg cctgtccatc atcgaccttt
      781 ccaccggcca gcagccgctc tacaacgaag accaaagtgt ctgcgtcgtc ttcaacggtg
      841 aaatctacaa ctatcagacg ctcattcccg aactccaggc cctcggccac actttccaca
      901 cccgcagcga cactgaagtc atcgtgcatg cctgggaatc ctggggcgaa aagtgcgtcg
      961 accgcttccg cggcatgttc gcctttgccc tctgggatcg caaccgtgaa accctcttcc
     1021 tcgcccgcga ccgcctcggc gtcaagccgc tctactacgc cctgcttgac gacggcacct
     1081 tcctcttcgg ctccgaactc aagtccatcc ttgcccatgg cggcctcaaa cgcgaaattg
     1141 atccctgcgc cgtcgaagaa tacttcgcct tcggttatgt tgccgaaccc cgcgccatct
     1201 tcaaacaagc caaaaagcta ccacccgccc acacgctact gctgcgccgc ggccaaccag
     1261 taggcgaacc gcgtgaatac tgggatgtcc acttcaccct cgacaatccg atcagcgacg
     1321 ccgatgcctg cgccgaactc acccaccgcc tcgaagaatc aatccgcctt cggatgattt
     1381 ccgaagtccc gctcggcgcc ttcctttccg gcggtgttga ttccagcgcc gtggtggcca
     1441 tgatggccgg gctctcaaat gctgcggtca acacctgctc catcgggttt tccgatccag
     1501 ccttcaacga atccgaattc gccaaaatag tggcgg
//
LOCUS       DAORVN010000611         1532 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_98558_length_1532_cov_4.109005, whole genome shotgun sequence.
ACCESSION   DAORVN010000611 DAORVN010000000
VERSION     DAORVN010000611.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1532
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98558_length_1532_cov_4.109005"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="PKV23_10825"
     CDS             complement(<1..184)
                     /locus_tag="PKV23_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007629624.1"
                     /note="responsible for the amidation of carboxylic groups
                     at position A and C of cobyrinic acid or hydrogenobrynic
                     acid; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinic acid a,c-diamide synthase"
                     /protein_id="HQX85973.1"
                     /translation="MLTLGLLRAFRNQGLRVASAKVGPDHIDPRFHEAASGRPCLNLD
                     PWAMKREVIASLLSEIN"
     gene            complement(228..1058)
                     /gene="cobA"
                     /locus_tag="PKV23_10830"
     CDS             complement(228..1058)
                     /gene="cobA"
                     /locus_tag="PKV23_10830"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014133067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HQX85974.1"
                     /translation="MVKPATAGFARLDGEAFPVFEAGWVWLVGAGPGAPGLMSLLCYH
                     AMQNCDVVVYDALVNPDILRWVRQGVSLEYAGKRGGKPSPVQQDISLRLIELAHAGRR
                     VLRLKGGDPFMFGRGGEESQALAKAKIPFRIVPGITAGVGGLAYAGIPSTHRDTNHSV
                     IFLTGHDATGKMPANVNWQAVATASPVIVMYMAVKNLGEIAQTLLEGGRAPNDPVTII
                     SNASLPHQQVQATTLDQAGAYVAKNDPPTPAIVVVGHASDWREMLDWYSPALRENAIG
                     "
     gene            1119..>1532
                     /locus_tag="PKV23_10835"
     CDS             1119..>1532
                     /locus_tag="PKV23_10835"
                     /EC_number="2.1.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015928827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-5B (C(1))-methyltransferase"
                     /protein_id="HQX85975.1"
                     /translation="MRIEKPEGELRRGWTTGACAAAATKAAFAALRTGTFPDPVSIRL
                     PKGETPSFALALEGRGEGWAQAGIIKDAGDDPDVTHGCTVIATVRSRGKGVTFKAGKG
                     VGTVTRAGLPIAVGEPAINPVPREMMRAVIEEQGGG"
BASE COUNT          288 a          444 c          533 g          267 t
ORIGIN      
        1 ggttaatttc gcttaacagg ctggcgatga cttcccgctt catggcccaa gggtcgaggt
       61 tgaggcaggg cctgccgctg gccgcctcgt ggaacctggg atcgatatga tcggggccga
      121 cttttgccga ggccacacgg agcccttgat tccggaaagc gcggagcagg ccaagggtga
      181 gcatggtctt gccgctgcct gaggatgggg ccgcaacgac aaaggcctta gccaatggcg
      241 ttctcccgga gtgccgggct gtaccaatcg agcatttcac gccagtcgga ggcatggccg
      301 acgacgacga tggccggggt gggcggatcg tttttggcca cataggcccc ggcctgatcg
      361 agcgtcgtag cctgcacctg ttgatgcggg agggaggcgt tggaaatgat ggtgacgggg
      421 tcattgggtg ccctgccgcc ttccagcaag gtttgagcaa tctcgcccaa gttcttgact
      481 gccatataca ttacgatcac gggggaggcg gtggccacag cctgccagtt gacattggca
      541 ggcattttcc cggtggcgtc atggccggtc aagaagatca cggaatggtt ggtatcccgg
      601 tgggttgagg ggattccggc ataggcaagc ccgccgacac cggctgttat gcccgggaca
      661 atgcggaagg ggattttggc cttggccagg gcctggcttt cctcgccgcc gcggccgaac
      721 atgaagggat cgccgccctt gaggcgcagg actcgtctgc ccgcatgggc cagttcgata
      781 aggcgcagcg aaatatcctg ctggacgggc gagggcttgc cgccgcgctt gccggcatat
      841 tcgagactca cgccctgcct cacccagcgc aggatatccg gattgaccag ggcatcgtaa
      901 acgacgacat cgcagttctg catggcgtgg tagcagagca gcgacatgag gcccggagct
      961 ccggggccgg cacccaccag ccagacccag cccgcttcaa acaccggaaa ggcttcgcca
     1021 tcgaggcggg caaatccagc cgtcgcgggc ttaaccacgg gcggctgatg cgctgatttt
     1081 gttggttcgc ggctcatagt tccttataca gtgatcgcat gcgaatagag aagcccgaag
     1141 gggagttaag gcgcggctgg accacgggtg catgtgcggc ggcggcgacc aaggcggcgt
     1201 ttgcggcatt gcgcaccggc acgtttcccg atccggtcag catcaggctt ccgaaaggcg
     1261 aaacaccgag tttcgccctc gcgcttgagg gccggggcga aggctgggcg caagccggga
     1321 tcatcaagga cgcaggcgac gatccggatg tgacgcacgg ctgcacggta atcgccacgg
     1381 tgcgaagccg gggcaagggc gtgaccttca aggcaggcaa gggcgtaggc accgtcacgc
     1441 gggcagggct tcccattgcg gtgggcgaac ccgccatcaa tccggtcccg cgcgagatga
     1501 tgcgcgccgt gatcgaagag cagggcggcg gc
//
LOCUS       DAORVN010000612         1530 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_98820_length_1530_cov_2.653559, whole genome shotgun sequence.
ACCESSION   DAORVN010000612 DAORVN010000000
VERSION     DAORVN010000612.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1530
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98820_length_1530_cov_2.653559"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..141)
                     /locus_tag="PKV23_10840"
     CDS             complement(<1..141)
                     /locus_tag="PKV23_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HQX85976.1"
                     /translation="MKHEHTHSTHNPSATGVIDPVCGMTVDPATAKHKAEHQGQSYYF
                     CSA"
     gene            231..506
                     /locus_tag="PKV23_10845"
     CDS             231..506
                     /locus_tag="PKV23_10845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011505174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HQX85977.1"
                     /translation="MQKDTKAAVLKRLSRIGGQVGGLSRMVEEDRYCIDVVTQISAVR
                     AALRRAEEEILRDHVSHCVEHAIASGNKAEQRRKISEIIDVLGRAER"
     gene            complement(503..1156)
                     /gene="msrA"
                     /locus_tag="PKV23_10850"
     CDS             complement(503..1156)
                     /gene="msrA"
                     /locus_tag="PKV23_10850"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019199029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HQX85978.1"
                     /translation="MIFARRKAEMTDAARALPGRASAIATASHHFVNGNPLKGPYPAG
                     SEQIVVAMGCYWGAERTFWELPGVWVTAVGNAGGFTPNPTYEEVCSGLTGHTESVLVV
                     FEPSKVSLNEVLKVFWEAHDPTQGMRQGNDTGTQYRSAIYWNSPAQRDAVLASKAAYQ
                     KALTAQGYGEITTEIAEAGPFYFAETYHQQFLAKNPHGYCGIGGTGVVCQIGTGVEG"
     gene            1297..1387
                     /locus_tag="PKV23_10855"
     tRNA            1297..1387
                     /locus_tag="PKV23_10855"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1331..1333,aa:Ser,seq:gga)
BASE COUNT          294 a          467 c          446 g          323 t
ORIGIN      
        1 cgccgaacag aagtagtaac tctggccctg atgctccgcc ttatgcttgg ccgtcgcagg
       61 gtccaccgtc atcccgcaga caggatcgat gacccccgtg gcagatgggt tatgcgttga
      121 atgcgtgtgc tcatgtttca tttgcggcct gcctttgtct gagtcctgca caccctatgg
      181 gggtataatc gatgcttgac agatataccc cccataggta tatgtcaatc atgcagaaag
      241 ataccaaagc tgccgtgctg aaacggctca gccgcatcgg cggccaggtg ggcggcctct
      301 cccgcatggt tgaggaagac cgctactgca tcgatgtcgt cacccagatt tcagcggtgc
      361 gggcggcgct gcgccgtgcc gaggaggaaa tcctccgcga tcatgtcagc cattgtgtcg
      421 agcacgccat tgccagcggc aataaggcgg agcagcgccg gaagatttcc gagattatcg
      481 acgtcctggg ccgcgccgag cgctagcctt caaccccggt gccgatctgg cagacaacgc
      541 cggtgccgcc aatcccgcaa tagccgtgcg gattcttggc gagaaactgc tggtgatagg
      601 tctcggcgaa atagaaaggc ccggcctcgg caatttccgt ggtaatctct ccgtagccct
      661 gcgccgtcag cgctttctgg taggccgcct tcgaggccag caccgcgtcg cgttgcgccg
      721 gcgagttcca gtagatcgcc gaacggtatt gcgtgcccgt gtcattgccc tggcgcatcc
      781 cttgcgtggg gtcatgcgct tcccagaaca ctttcaaaac ctcgttgagg ctcaccttgc
      841 tgggttcaaa caccaccagc accgattccg tgtgccccgt gagcccggaa cagacctcct
      901 cataggtagg gttcggcgta aagccgccag catttcccac cgctgtcacc cagacgccgg
      961 gcagctccca gaacgtgcgc tccgcccccc aatagcagcc catagccacg acaatctgct
     1021 cggaccctgc ggggtatggc cctttcaacg ggtttccgtt gacgaagtgg tggctggccg
     1081 tggcaatggc gcttgcccgc ccgggcaggg cccgggcggc atcggtcatt tcggctttcc
     1141 tgcgggcgaa aatcatggat tcctgtctcc ttttatcttg agacccattt agtgtggcag
     1201 atcggtaact tccattcaac aacgcttgaa cggcagagtt tcatcgctat agtgcggcgc
     1261 aattcgacgg gggcccaggc gcccccaaat ttctgcggaa gggtggccga gtggcttaag
     1321 gcgcacgcct ggaaagcgtg tttgcgttaa tagcgtaacg ggggttcgaa tcccccccct
     1381 tccgccatcc tgcgccacgg cttagggtgg caggccaggc ttcaactcac tgatttggaa
     1441 cagatttaac gatctggtaa gctatgggcg caggttcacg ggcggttaat ctgcccagtg
     1501 gcaaaccatt aagcatgagg cgccaaaatt
//
LOCUS       DAORVN010000613         1529 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_98896_length_1529_cov_3.785617, whole genome shotgun sequence.
ACCESSION   DAORVN010000613 DAORVN010000000
VERSION     DAORVN010000613.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1529
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98896_length_1529_cov_3.785617"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1529
                     /locus_tag="PKV23_10860"
     CDS             <1..>1529
                     /locus_tag="PKV23_10860"
                     /inference="COORDINATES: protein motif:HMM:TIGR00358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - exoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="VacB/RNase II family 3'-5' exoribonuclease"
                     /protein_id="HQX85979.1"
                     /translation="NSATWAQVRRAAIIEKEKEMSIKKLSKVRRADPFFERESVRYEF
                     PLPSREYVTQMLTDEARPLSFVELVDLLDIAESEREMFQRRLGAMEREGQLLRNRKGT
                     YILPERASLTAGKIQGHQDGYGFLIPDDGSPDIFLAQHQMAKVLHGDRALVRIMGVDR
                     KGRPEGSIVEVTERANTRVVGRVLVEHGLVFVVPENRRIAQDILVPVEKKAKIKPESG
                     MVVMVDIIEQPSKFSKPIGQITEVLGNYADPGMEIEIALRKHDLPFEFSRAALEENKA
                     LPDKVRKADLSGREDLRDLPLVTIDGETARDFDDAVFCEKKGRGWRLVVAIADVSHYV
                     KPGMALDKEAMERGNSVYFPRRVIPMLPEKLSNGICSLNPDVDRMAMVCDMDISAAGK
                     IGKYRFYPAVFRSKARLTYNLVWSWLSGEAQPENEILLALQPHIKNLYKLFQALHKAR
                     EQRGAIDFETTETQMVFNEQGKIENIVPVVRNDAHRVIEECMLAANVCASDFLSEHKQ
                     A"
BASE COUNT          324 a          368 c          507 g          330 t
ORIGIN      
        1 caattccgcc acctgggcac aggtgcgaag agccgctatt atagagaaag aaaaagagat
       61 gtcaataaaa aaactgtcta aagttcgtcg ggccgatccc ttctttgagc gggagtccgt
      121 ccgctatgaa tttcccttgc cttcgcgcga gtatgtgacg cagatgctga ctgacgaagc
      181 gcggccactg agttttgtcg agctggttga tttgctggat attgccgaaa gcgaacggga
      241 aatgttccag cgccggctgg gggcgatgga gcgggaaggg cagttgttgc gcaaccgcaa
      301 ggggacctac atcctgccgg agcgggccag cctgacggcg ggcaagattc aggggcatca
      361 ggacggttac ggatttctga ttccggatga cggcagtccc gatattttcc tcgcccagca
      421 tcaaatggcc aaggtattgc atggcgaccg cgctttggtg cggattatgg gcgttgaccg
      481 caagggccgg ccggagggca gcattgtcga ggtgaccgag cgggcgaata cccgtgtcgt
      541 cggccgggtg cttgttgagc acggcctggt ctttgtggtg ccggagaatc gccggatcgc
      601 ccaggatatt ttggtgccag tggagaagaa agcgaagata aagccggagt cgggcatggt
      661 ggtgatggtc gatatcatcg aacagccaag caaattctcg aaaccgattg ggcaaattac
      721 cgaggtgctg gggaattatg ccgatccggg catggaaatc gagattgcac tgcgcaagca
      781 tgatctgcct ttcgagtttt cccgagccgc tctggaggag aacaaggcgc tgccggacaa
      841 ggtcaggaag gctgatctgt ccgggcgtga ggatttgcgc gatttgccgc tggtcactat
      901 cgacggcgaa acggcgcgtg atttcgacga tgcggtgttc tgcgagaaga aggggcgtgg
      961 ctggcgcctg gtggtggcga ttgccgatgt ctcccattac gtgaagccgg gtatggcgct
     1021 cgacaaggag gcgatggagc ggggaaattc ggtctatttc ccgcgtcggg tgattccgat
     1081 gctgccggaa aagttgtcga acggcatctg ctcgttgaat ccggacgttg atcggatggc
     1141 gatggtttgc gatatggata tcagcgccgc cggcaagatt ggtaagtacc gcttttatcc
     1201 ggcggttttc cgttccaagg ctcggctgac ttacaacctg gtctggtcat ggctgtctgg
     1261 cgaggcgcag ccggagaacg agattcttct ggcgctgcag ccgcatatca agaatcttta
     1321 caagcttttc caggcgctgc acaaggcgcg ggagcagcgc ggggcgattg atttcgagac
     1381 gactgaaacg cagatggttt tcaacgagca gggcaagatc gagaacatcg tgcccgtggt
     1441 gcgcaatgat gcgcatcggg ttattgagga atgcatgctg gcggccaacg tctgcgcctc
     1501 cgactttctc tctgaacaca aacaggctt
//
LOCUS       DAORVN010000614         1528 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_99015_length_1528_cov_3.492872, whole genome shotgun sequence.
ACCESSION   DAORVN010000614 DAORVN010000000
VERSION     DAORVN010000614.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1528
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99015_length_1528_cov_3.492872"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="PKV23_10865"
     CDS             complement(<1..264)
                     /locus_tag="PKV23_10865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013421081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphatase family protein"
                     /protein_id="HQX85980.1"
                     /translation="MPGDLPHAVLFACTQNVIRSPMAAAIMKHFYGHRVYVASAGVKP
                     GDPDPFIATVLDEIGIDLGKHRPHNFSDLEDSSFDLAISLSPEA"
     gene            complement(274..753)
                     /locus_tag="PKV23_10870"
     CDS             complement(274..753)
                     /locus_tag="PKV23_10870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007200106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0262 family protein"
                     /protein_id="HQX85981.1"
                     /translation="MTTLKKNRLIAVDLDASIGPGPSAEAEHERRVAIYDLIEENTFG
                     LLNGKEGPYRLQLSTQDGRLMFEIFNEQEEKLSIIGLSMSPFRVIVKDYFQICESYYQ
                     AIRHSTPSQIETIDMARRGLHNEGSEILKERLNGKVDIDFDTARRLFTLVCVLHWRG"
     gene            complement(787..1200)
                     /locus_tag="PKV23_10875"
     CDS             complement(787..1200)
                     /locus_tag="PKV23_10875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007873432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2948 family protein"
                     /protein_id="HQX85982.1"
                     /translation="MLRLTAKDSEDLKVISAHMQDALVRLADIRYLAKSRQFAFVANR
                     FAWELQPKAERRRSGLHFEHVLSVKQHGIMAQAKDAVLSLLAITFEPGPEPSGTVVLT
                     FSANCTIRLEVEYLDLHLKDLGGAWATHLTPHHGD"
     gene            complement(1204..>1528)
                     /locus_tag="PKV23_10880"
     CDS             complement(1204..>1528)
                     /locus_tag="PKV23_10880"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002965536.1"
                     /note="adds enolpyruvyl to UDP-N-acetylglucosamine as a
                     component of cell wall formation; gram-positive bacteria
                     have 2 copies of MurA which are active; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HQX85983.1"
                     /translation="LMTKAKGTAMIRETIFENRFMHVQELARLGADIHLHGDTAEVRG
                     VQKLTGADVMATDLRASVSLVIAGLVAEGETMIHRVYHLDRGFEALEEKLQACGADVE
                     RLAGN"
BASE COUNT          310 a          448 c          460 g          310 t
ORIGIN      
        1 cgcctccggc gacagcgaaa tggcgagatc gaagcttgaa tcttccaggt cggaaaaatt
       61 gtgcggccgg tgcttgccaa ggtcgatgcc gatttcatcg agcacggttg cgataaaggg
      121 atcgggatcg ccgggcttga cgcccgccga ggccacgtaa accctgtgcc cgtagaagtg
      181 cttcatgatg gccgccgcca tcggcgagcg gatgacattt tgcgtgcagg caaagagcac
      241 ggcatgcggg aggtcacccg gcatcggcaa atcctatccg cgccaatgca ggacacagac
      301 cagcgtaaac agccgccgcg ccgtatcaaa atcgatgtcg accttgccgt tgagcctttc
      361 cttgagaatt tcgctgccct cgttgtgcaa cccgcgccgc gccatgtcga tggtttcgat
      421 ctgcgagggc gtggagtgcc tgatggcctg gtaatagctt tcgcaaatct ggaaatagtc
      481 cttcacgatg acccggaacg gcgacatgga aagcccgatg atcgacagct tttcttcctg
      541 ctcattgaag atttcaaaca tcaggcgccc gtcctgggtg gacagctgca ggcggtaagg
      601 cccctccttc ccgttcagca ggccgaacgt gttttcctcg atcaggtcat agatggcgac
      661 gcgccgctca tgctcggcct cggccgatgg gcctggcccg atgctggcgt caagatcgac
      721 agcaatgagg cggtttttct taagagttgt catgaattga gattagacgg aaagctcgca
      781 aaggcgctag tcaccgtgat gaggggtcaa atgggtggcc caggccccgc caaggtcctt
      841 caaatgcaga tcgagatatt cgacctcgag cctgatggtg cagttggccg agaacgtgag
      901 aaccaccgtg ccggatggct ccgggccagg ttcaaaagtg attgcaagaa gcgacaggac
      961 agcgtctttc gcctgggcca tgatgccgtg ctgcttcacg ctgaggacgt gctcgaaatg
     1021 caggccgctg cgccgccgct cggctttcgg ctgcagctcc caggcaaagc ggttggccac
     1081 gaaggcaaac tgccgtgact tggccaggta gcggatgtcg gcaagccgca ccagggcatc
     1141 ctgcatgtgg gcagaaatga ccttcagatc ttccgagtct ttcgccgtaa gcctcagcac
     1201 caatcagttt cccgcaagcc tttcgacatc ggcgccgcag gcctgcagtt tttcttccag
     1261 cgcttcaaag ccgcggtcaa gatggtagac gcggtggatc atcgtttcac cttccgccac
     1321 gaggccggcg atgaccagtg acaccgaggc gcgcaggtcg gtggccatca cgtcggcacc
     1381 ggtaagcttt tgcacgccgc gcacttccgc cgtgtcgcca tgcaggtgga tatcggcgcc
     1441 cagccgggcc agttcctgca catgcatgaa acggttttca aagatcgttt cgcgaatcat
     1501 ggcggtgccc ttggccttgg tcatcagc
//
LOCUS       DAORVN010000615         1526 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_99252_length_1526_cov_3.035350, whole genome shotgun sequence.
ACCESSION   DAORVN010000615 DAORVN010000000
VERSION     DAORVN010000615.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1526
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99252_length_1526_cov_3.035350"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..548)
                     /locus_tag="PKV23_10885"
     CDS             complement(<1..548)
                     /locus_tag="PKV23_10885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/Srx family N-terminal domain-containing
                     protein"
                     /protein_id="HQX85984.1"
                     /translation="MDLVFAPSQIETWPLDRLRPYARNAKIHGTDQVAKIAASMAKFG
                     WTVPCLVADDGELIAGHGRVLAAIMLGLTDVPVIRLGHLDEAERRAYRIADNKLTELG
                     EWDEAILRDEIAGLLAEDFDLSLLGITDEDLDALLRDPDQVEGGAVEGEDDIPDPPVT
                     PVSVAGDLWQLGSHRLICGDSTS"
     gene            1148..>1526
                     /locus_tag="PKV23_10890"
     CDS             1148..>1526
                     /locus_tag="PKV23_10890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HQX85985.1"
                     /translation="MKLISNSGTERALDELKSWSTDGAAIDIMSSSFSVHAFDAVADQ
                     LERGGKCRMILGNGDVVADSLFGGPEETALRARLKGRWLAGRASHWLKRAAEIRHTKV
                     SPPQSLIVGGPSLERKALLGSCSF"
BASE COUNT          286 a          460 c          480 g          300 t
ORIGIN      
        1 gaagtgctgt caccgcagat cagccggtgc gacccgagct gccacaggtc gcccgccacc
       61 gacacgggcg tgaccggcgg atcgggaata tcatcctcac cctcgaccgc gccgccttcc
      121 acctgatccg gatcccgcag cagcgcatcc agatcctcgt cggtgatccc cagcagcgac
      181 agatcaaaat cctcggccag cagccctgcg atctcgtcgc gcaaaattgc ctcgtcccac
      241 tcgcccaact cggtcagctt gttgtcggcg atgcggtagg cccggcgttc ggcttcgtcg
      301 aggtggccga gccggatcac cggtacgtcg gtcagcccca gcatgatggc ggccaacacc
      361 cggccgtgcc cggcgatcag ctcgccatcg tcggccacca agcagggcac ggtccagccg
      421 aacttggcca tgctggcggc gatcttggcg acctggtccg tgccgtggat cttggcattg
      481 cgggcatagg ggcgcagccg gtcgagcggc caagtctcga tttggctcgg tgcgaagacc
      541 aggtccatgg ttggctctct cgggcagggc gggcaagccg atgcacgctg ggcgatgcca
      601 gcgtcaggat cgggatccgc gatgtcggga aatgagaaac gcccgcgagg gggttcctcc
      661 gggcgcaatt cttcgatgat caaggggtag gtcaaggggg gcagctttgt caaatgaaaa
      721 atgcacgcgg attcaatggc ttccctgcaa gtggcttccg ctggctggct tccggcaagg
      781 tggcttccgc aaactggatt ccctggattc cgcaaagaat ccagcgcgcc aagatcgtga
      841 ttccgcaagc ctttgataat gagtcgcttt ttccacgatc aaccgtcagg tggattccgc
      901 ctggcttccc cggtgaaact gcctgtcgct agcgaaacgc cgcgctgcgc ccccccgcat
      961 acaaacgggg ccggggagga accatgccgt gggggaccgg taacactttg cccgcggcca
     1021 tgttgaatca gcattgacgg ccctggtcct aacgctttgt gttgacgcag tttcctccgc
     1081 tcttaggagg gaagtcagga ttcggcgcgc cgtgcgcttc gaaggcaata ggtggagtag
     1141 gaggcccatg aagctcatca gcaatagcgg aacagaacgt gcactcgatg agctgaagtc
     1201 atggtccacc gatggggcgg cgattgatat catgtcgtca tccttttcgg tgcacgcatt
     1261 tgacgccgtt gcggatcagc ttgagcgggg cggtaaatgc cgcatgatcc tcggcaatgg
     1321 ggatgttgta gcggatagtc tctttggcgg acccgaagaa acagccctgc gtgcaaggct
     1381 aaaggggcgc tggcttgcag ggcgtgcttc gcattggttg aagcgggcgg ctgaaatcag
     1441 gcacaccaag gtttctccac ctcaatcact gatcgtcggt gggccttctc ttgagcgcaa
     1501 agcacttctg ggatcctgct ccttct
//
LOCUS       DAORVN010000616         1523 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_99614_length_1523_cov_2.470027, whole genome shotgun sequence.
ACCESSION   DAORVN010000616 DAORVN010000000
VERSION     DAORVN010000616.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1523
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99614_length_1523_cov_2.470027"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1523
                     /locus_tag="PKV23_10895"
     CDS             <1..>1523
                     /locus_tag="PKV23_10895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006725447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQX85986.1"
                     /translation="ILVNCAGLWAKRVGNMAGVALAAGIVEHQYFLTEKTLSFDAGLT
                     TLRDPDKNFYLKPDTGSFAIGGWEEGSRGFHRGLPPLDFGRELFAPNMERLELFALPA
                     SERLPVLNETGIQAVINGPIPVSADGEPIMGLAPELDNFYVACGFTAGIAASGGAGLA
                     MANMILHGDAGMDLWPFDVRRFSGVHAQPRYLETRAIEAYAAYYKVHWPLEESHAGRN
                     LRLSPLHETLKKAGAVFGSRAGWERPNWFAPAGVDPVDVPSFEDRPNWFSHVGAECRA
                     IRERAVLIDQTSFAKFEITGPGTQAALERIAANDLSGPPGKAVYTQLCNGKGGIEADV
                     TLVHAGPDHFYLITGSGFGVRDSGWVAKHLPPSVQIRDVTNALAVINICGPRSRDILQ
                     SVTDADISNVAFPFFGARRIDVGHAAVLAVRVGYVGELGYELYIPQEFGAHVYDTLWE
                     AGKAHGIANAGYRAIDSCRMEKGYLYWSGDISLDYNPYEAGLGFCVALDKSDFIGRE"
BASE COUNT          270 a          415 c          506 g          332 t
ORIGIN      
        1 tatcctcgtg aactgcgccg ggctttgggc caagcgggtg ggcaatatgg cgggcgttgc
       61 gcttgcggcg gggattgtgg aacaccagta tttcctcacc gagaaaaccc tgtcgtttga
      121 tgcgggactc acgacgctcc gcgacccaga caagaatttc tacctgaaac cggatacggg
      181 ctcctttgcc attggcggct gggaagaagg ttcccgcggc tttcatcgcg ggcttccgcc
      241 gctggatttt ggccgcgaac tctttgcgcc aaacatggaa cggctggaac tttttgcgct
      301 gccggcttcg gagcgcctgc cagttctcaa tgaaacaggc atccaggcgg tgattaacgg
      361 tcccattccg gtttccgccg atggcgaacc gatcatgggg ctggcgccgg agcttgataa
      421 tttctatgtg gcctgcggct tcacggcggg cattgcggct tcgggcggag ccggccttgc
      481 aatggccaac atgattctac atggcgatgc gggcatggac ctctggccat ttgatgtgcg
      541 gcgtttcagc ggggttcatg cgcagccgcg ctacctggag acacgcgcca ttgaagccta
      601 cgcggcctat tacaaggttc attggccgct tgaggagagc catgcggggc gcaacttacg
      661 gctttcaccc ctgcatgaga cattgaagaa agccggggcc gttttcggct cgcgggcagg
      721 ctgggagcgg ccgaactggt ttgcgccggc gggtgtggac ccggttgacg ttccctcctt
      781 tgaagacagg ccgaactggt tttcgcatgt gggcgcggaa tgccgggcca tccgcgaacg
      841 cgcggtgctg attgaccaga cgtcctttgc gaagtttgag attactggtc ccgggactca
      901 agcggcgctg gagcggattg cggccaatga cctttcaggc ccgccgggga aagcggttta
      961 cacgcaactc tgcaacggga agggcggcat cgaggcggat gtgacgctgg ttcatgcggg
     1021 ccccgatcat ttctatctga tcaccggttc gggcttcggg gtgcgcgaca gcggctgggt
     1081 ggcgaaacat ctgccgccat cagtacagat ccgcgacgtc accaacgcgc tggcagtgat
     1141 caatatctgc gggccgcgat cacgcgacat cctgcaatcg gtgaccgatg cggatatctc
     1201 caatgtggcg tttccgtttt tcggagcgcg gcggattgat gtgggccacg cagcggtgct
     1261 tgcggtgcgc gtgggctatg tgggcgagct tggctatgag ctttacattc cacaggagtt
     1321 cggcgcccat gtttatgaca cgctgtggga ggcaggaaag gcccatggca tcgccaacgc
     1381 gggttaccgg gcgattgaca gctgccgcat ggagaagggc tatctctact ggtctggtga
     1441 tatttcactg gactataacc cttatgaggc gggactgggt ttctgtgtcg ccctggacaa
     1501 gagtgatttc atcgggcgcg agg
//
LOCUS       DAORVN010000617         1513 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_100727_length_1513_cov_3.728395, whole genome shotgun
            sequence.
ACCESSION   DAORVN010000617 DAORVN010000000
VERSION     DAORVN010000617.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100727_length_1513_cov_3.728395"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..749
                     /locus_tag="PKV23_10900"
     CDS             <1..749
                     /locus_tag="PKV23_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008840649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HQX85987.1"
                     /translation="LAGTLVQAVAECLFGLVYINCVVPGAVAIWGPWPFVSDLRTGAM
                     SGGSGEQALITSGCAQMGHFYNLTVGSAAGMCDSKLPDMQAGYEKGVTAGISAMTGVN
                     LMYESAGMHASLLGFCLESLLIDNDMLGAINRNVRGIEVNEDTLSVEVIREVCLNGPG
                     HFLGSDQTLNLMQKEYIYPKIANRMSPKEWIEAGRPDLVERAAARKREILSTHYPDYL
                     PRAMDDVIRARHDIKLPREVMEMGDPRWIK"
     gene            756..1427
                     /locus_tag="PKV23_10905"
     CDS             756..1427
                     /locus_tag="PKV23_10905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85988.1"
                     /translation="MEHPILTSIRRAGFAPLGWFEPGAADAVPHDARFVILVGNAGPE
                     MFRRFALERDHRTAKIDDWTRDVLGGLADDLSAKAVFPFDTPPLPFLTWARRARAGHV
                     SPLGLNIHPTYGLWHAFRAALLFPAAFDLPLQSAGAHPCETCADKPCLSACPVHAFNG
                     SSYDVAACAAHLETPAGEDCMSGGCLARRACPVGREFTYHPFQAHFFMRAFREARRNP
                     AGNAA"
BASE COUNT          271 a          453 c          472 g          317 t
ORIGIN      
        1 ccctggcggg cacgctggtt caggcggtag ccgagtgcct gttcggcctg gtctatatca
       61 attgcgtggt tccaggcgct gttgccatct gggggccatg gcccttcgtg tccgacttgc
      121 gcaccggggc catgtccggc ggttcgggcg aacaggcctt gatcacctcg ggctgcgccc
      181 agatgggcca tttctacaat ctcacggtag gctccgccgc aggcatgtgc gattcaaaac
      241 tgccggacat gcaggcgggc tatgaaaagg gtgtgaccgc cggcatctcg gccatgacgg
      301 gcgtgaacct gatgtatgaa agtgccggca tgcacgcttc gctcctgggt ttctgccttg
      361 aaagcctgct catcgacaac gacatgctgg gagcgatcaa ccgcaatgtg cgcggcattg
      421 aagtgaatga ggacacattg tcggtcgagg tcattcgcga ggtttgcctg aatggcccgg
      481 ggcatttcct cggcagcgac caaacgctga acctgatgca gaaggaatac atatacccca
      541 agatcgccaa ccggatgagc cccaaggaat ggatcgaggc gggccggcct gacctggttg
      601 agcgggcggc ggcgcgcaag cgggaaattc tctcgacgca ttatcccgac tatctgccgc
      661 gggcgatgga tgacgtgatc cgggcgcgcc atgacatcaa actgccacgc gaggtcatgg
      721 agatgggcga cccgcgctgg ataaaatagg ccgatatgga acatccgata ctcaccagca
      781 tccggcgggc aggattcgcg cccttgggct ggtttgagcc cggcgccgcc gatgcggttc
      841 cccatgatgc caggtttgtc attctcgtcg gcaatgcggg gcctgaaatg tttcgccggt
      901 ttgcccttga acgcgatcac cggacggcaa aaatcgatga ctggacacgc gacgtcttgg
      961 gtggccttgc cgacgacctt tcggcaaagg cggttttccc attcgacacg ccgcccctgc
     1021 cctttctgac ctgggcgcgg cgggcgaggg ctggccacgt ttcgccgctt ggcctcaaca
     1081 ttcacccgac atacgggctg tggcatgcct ttcgcgccgc actgcttttt ccggcggcgt
     1141 ttgatttgcc tttgcaatcg gctggggcgc acccttgcga gacttgcgcc gacaagccct
     1201 gtctttcggc ctgcccggtc catgccttca atggctcttc ctatgatgtt gcggcatgtg
     1261 ccgcccattt ggaaacgccg gcgggtgagg attgcatgtc cggcggttgc ctggcgcggc
     1321 gggcgtgccc ggtgggccgg gaattcacct accatccttt ccaggcgcat ttcttcatgc
     1381 gggcatttcg cgaggcacgt cgcaatccgg ctggaaatgc tgcctaggcc ggctattgtt
     1441 aagccatgta caagaacatt caaaagaaac tcgatctcgg ccagaccatc atccttgatg
     1501 gcggcacggg cac
//
LOCUS       DAORVN010000618         1508 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_101339_length_1508_cov_3.342051, whole genome shotgun
            sequence.
ACCESSION   DAORVN010000618 DAORVN010000000
VERSION     DAORVN010000618.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1508
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101339_length_1508_cov_3.342051"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(134..1027)
                     /gene="puuE"
                     /locus_tag="PKV23_10910"
     CDS             complement(134..1027)
                     /gene="puuE"
                     /locus_tag="PKV23_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008552039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase PuuE"
                     /protein_id="HQX85989.1"
                     /translation="MTDYPRDLKGYGRAIPDPKWPDGARIAVQFVLNYEEGGENNILH
                     GDAASEAFLSETVGAAPWPGQRNWNMESVYEYGARAGFWRLWRMFTGRKIPLTIYGVA
                     TALQRSPEQTQAMIEAGWEIASHGLKWIDYKDMPEDEEWRHIQDAIRIHKEVTGERPL
                     GWYTGRCSMNTQRLIRQEGGFLYSSDSYADELPYWVEKHLVIPYTLDANDMRFAMAQG
                     FNTGEQFFQYLKDSFDCLYEEGKRGSPKMMSVGLHCRLAGRPGRALGLAKFLDYVQSH
                     DKVWIARRVDIARHWHSTFPV"
     gene            complement(1008..>1508)
                     /locus_tag="PKV23_10915"
     CDS             complement(1008..>1508)
                     /locus_tag="PKV23_10915"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HQX85990.1"
                     /translation="VHLSESECQRLHEAGATIAHCPTSNNFLGSGLMSMKHLGKKERS
                     VPVALATDVGGGTTYSMLATLGEAYKVQMLTGYKPTALELFWMATRGNAERLRLGHEI
                     GTLETGKFADIVVLDQRATPVLAARQELSQSLEDMLFAMMILGDDRAIRATYVAGEKV
                     HDRLSA"
BASE COUNT          310 a          506 c          397 g          295 t
ORIGIN      
        1 cgccgccgcc ggaaagcgcg ccaggaaatc atgctccggg aaaagcatgg aaagattggc
       61 actgaatctg ggcatcgggt ggtctccgtc gtcgaatgat acttagcttc caaggaaaca
      121 cctgtcgatt cccctaaacc gggaaggtgc tatgccagtg ccgggcaata tcaacacgcc
      181 gcgcgatcca caccttgtca tgggactgca cataatcgag gaatttcgcc agccccagtg
      241 cgcggccggg ccggcccgcc aggcggcaat gcagccccac cgacatcatc ttgggtgagc
      301 cgcgcttgcc ctcctcatag aggcagtcaa atgagtcctt gagatactga aaaaactgct
      361 cacccgtatt gaagccctgc gccatggcaa agcgcatgtc attggcatcc agcgtgtagg
      421 gaatgaccag atgcttctcc acccaatagg gcaattcatc cgcataggaa tccgatgaat
      481 aaagaaaacc gccctcctgc cggatcagcc gctgcgtgtt catcgaacag cgcccggtgt
      541 accagccgag cggacgctct cccgtcactt ccttgtggat gcggatggca tcctggatgt
      601 gccgccactc ctcgtcttcc ggcatatcct tgtagtcaat ccacttcagg ccatggctgg
      661 caatctccca gcctgcctcg atcatcgcct gcgtctgctc cggcgaacgc tgcagggcgg
      721 tggccacgcc ataaatcgtc agcggaatct tgcggcccgt gaacatgcgc cacagccgcc
      781 agaaacccgc ccgcgccccg tactcgtaaa ccgattccat gttccagttg cgctgcccgg
      841 gccacggagc cgctcctacg gtttccgaca ggaaggcttc cgaagccgca tcgccatgca
      901 ggatattgtt ttcgccgccc tcttcgtagt tcaggacaaa ctgcacggcg atgcgcgcgc
      961 catccggcca tttcggatcc ggaatggccc ggccataacc cttgagatca cgcggatagt
     1021 cggtcatgca ctttttctcc cgccacatag gtagcacgaa tggccctgtc atcgcccaat
     1081 atcatcatgg cgaaaagcat gtcttccagc gactgcgaca gctcctgccg cgctgccaga
     1141 accggcgttg cccgctggtc cagcaccacg atatccgcaa atttccctgt ctcaagcgtc
     1201 ccgatttcat ggccgagccg cagccgctcc gcattgcccc gcgtggccat ccagaacagc
     1261 tccagcgccg tcggcttgta gcccgtcagc atctgaacct tgtaggcctc gcccagcgtt
     1321 gccagcatgg aataggtcgt gccgccgccc acatcagttg ccagcgcaac cggaacggac
     1381 cgttccttct tgcccagatg cttcatcgac atgagtccgg aaccgaggaa gttattcgac
     1441 gtggggcaat gggcaatggt cgctcccgcc tcatgcagcc gctggcattc gctttccgac
     1501 aaatgcac
//
LOCUS       DAORVN010000619         1506 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_101593_length_1506_cov_3.054445, whole genome shotgun
            sequence.
ACCESSION   DAORVN010000619 DAORVN010000000
VERSION     DAORVN010000619.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1506)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1506)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1506
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101593_length_1506_cov_3.054445"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(29..583)
                     /locus_tag="PKV23_10920"
     CDS             complement(29..583)
                     /locus_tag="PKV23_10920"
                     /inference="COORDINATES: protein motif:HMM:NF017319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HQX85991.1"
                     /translation="MMRGRWHVFLFILFLGAILPDATAKACPASSFVTNAGNAFLGAA
                     RVHTASAFSGVTSRYTDLRGISMFALGPHRARLSKSREAEYLALTRGFIGRFMMKHSA
                     RFSGSGMQIKDCVASSNALTVSTQLSNGKKIIFRLQRSKRGFLVRDVNVSSVWLAQQL
                     RSTFVGVINRNGGDINALFAYLRK"
     gene            complement(655..>1506)
                     /locus_tag="PKV23_10925"
     CDS             complement(655..>1506)
                     /locus_tag="PKV23_10925"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF039989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HQX85992.1"
                     /translation="PALQRAVIQAIEKMGGQRKLKKLYFQHQKSVAGGENFFDAAVRL
                     LKLNVMYDEAELALTPKTGPVLFIANHPYGVMDGITLTWLATKVRADTKVLANDVLCQ
                     APEAAGNLLPVAFAPTREARETNVNSRLAAQSWLREGHAVGIFPGGGVATSEKPLKGQ
                     AVDLPWAPFTAKLLRMSKATVVPIFFTGQNSRLFQLASHLSLTLRLSLVFRETTRRIG
                     TGLNVRIGRPVPFSEIAHIEDRNELVMELRKRTYDLAKAEDIKGTRTGLHLREAKIKG
                     GKYRDTE"
BASE COUNT          296 a          415 c          493 g          302 t
ORIGIN      
        1 ggtttttcct ggtggcgcaa gtctgaattc atttgcgcag gtaagcaaag agggcgttga
       61 tgtcgccgcc gttgcgattg atcactccca caaaggttga gcgcagctgc tgggcgagcc
      121 agaccgatga gacattgacg tcgcgcacca gaaagccgcg cttggagcgc tgcaacctga
      181 agatgatctt cttgccgttc gaaagctggg tgctgacggt cagggcgttg ctggaggcaa
      241 cacagtcctt gatttgcatt ccgctgccgg agaaacgcgc ggaatgcttc atcatgaagc
      301 ggccaatgaa gccacgggtg agggcgagat actcggcctc ccgtgatttg ctcaagcgcg
      361 cacggtgcgg gccgagggcg aacatggata tgccgcgcag gtcggtgtag cgggaggtga
      421 cgccggaaaa ggccgaggcg gtatggacgc gcgccgcgcc caggaaggca ttgcccgcat
      481 tggtgacgaa gcttgaagcg ggacaagcct tcgccgtggc atccgggaga atggcaccca
      541 gaaacaggat gaacagaaag acatgccagc gcccacgcat catctcggct ccgatcgctg
      601 ctgtttgaat ccctttgaat ctgagtcgca agccctcttt ggaaggcaag ccagttattc
      661 cgtgtcgcgg tacttgccgc ccttgatctt tgcctcgcgc aaatgaagcc cggtgcgggt
      721 gcccttgatg tcttccgcct tggcaagatc ataagtgcgc ttgcgcagtt ccatgaccag
      781 ttcgttgcgg tcctcgatat gggcaatttc actgaagggc acggggcggc cgatgcgcac
      841 gttcaggccg gtgccgatgc gcctcgtggt ttcgcgaaag acgagggata gcctgagcgt
      901 caggctcaag tggcttgcaa gctgaaacag ccggctgttc tgcccggtga aaaagatcgg
      961 cactacggtt gcctttgaca tgcgcaggag tttggcggtg aagggcgccc agggcaggtc
     1021 aacggcctgg cccttcaatg gcttctccga ggtcgcaacg ccgccgcccg ggaagattcc
     1081 aacggcatgg ccttcgcgca gccagctttg cgcggcgagg cgtgaattca cgttggtttc
     1141 gcgggcttcg cgggtcggtg cgaaggccac gggcagaaga ttgccggcgg cttccggggc
     1201 ctggcacaag acatcattgg ccagaacctt ggtgtcggcc cgcactttgg ttgcaagcca
     1261 ggtgagggta atgccgtcca tgacgccgta gggatggttg gcaataaaga gaaccggacc
     1321 ggttttcggc gtgagcgcca gttccgcctc atcatacatg acattgagtt tcagcaggcg
     1381 gacggcggca tcaaagaaat tctcgccgcc cgccacgctc ttctggtgct ggaaataaag
     1441 cttcttgagc ttgcgctggc cacccatctt ttcaatggcc tggatgacgg cgcgctgcag
     1501 ggcggg
//
LOCUS       DAORVN010000620         1506 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_101637_length_1506_cov_2.250861, whole genome shotgun
            sequence.
ACCESSION   DAORVN010000620 DAORVN010000000
VERSION     DAORVN010000620.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1506)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1506)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1506
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101637_length_1506_cov_2.250861"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..561)
                     /locus_tag="PKV23_10930"
     CDS             complement(<1..561)
                     /locus_tag="PKV23_10930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85993.1"
                     /translation="MPVLLIAAFLMTAAATLAVLGPRFDFSRPVEIPIDRATQSSQAR
                     YQADFPAVASLMVVRVSAENSTLSRNAAQLIAGKLEAQKSDIRQVFIPGLGDFYDRFG
                     FLYLPPEEINARVERVKRMKPLFQAIAASPNLAGLSTLVNQVAEAVQKGRSPQGIESL
                     FVQMSETVKNQVANRPAPMDWTRVAGL"
     gene            775..>1506
                     /locus_tag="PKV23_10935"
     CDS             775..>1506
                     /locus_tag="PKV23_10935"
                     /inference="COORDINATES: protein motif:HMM:NF013237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HQX85994.1"
                     /translation="MASNTGKNSGPLHESTVAVHGGEFVDPATRASSPNLVMSATFAP
                     TDVTGFSARDEKDYDGFVYGRVSSPTVRQLEQKLAALEGAEAALAYASGVAASHALIA
                     GRLSKGDHLLMPDSNYVGVAELTRDTLPRFGIEASFVDITDAKAVAAEIRPNTRMLWL
                     ETPSNPTMQITDIKALAALAHEKGVRDVVVDSTYATPIATKPLSLGADFVIHSLTKYI
                     GGHGDAMGGAVLGRKTDLDALNLEAT"
BASE COUNT          327 a          438 c          451 g          290 t
ORIGIN      
        1 gagccctgcc acgcgcgtcc aatccatggg tgctggccgg tttgctacct gatttttcac
       61 cgtttccgac atctgcacga acagcgattc aatgccctgc ggggacctgc ccttctgaac
      121 cgcttcggct acctgattta caagggttga aagcccggca agatttggcg aggcggcgat
      181 ggcctggaac aacggtttca tgcgcttgac acgctcgacc cgggcattga tttcctccgg
      241 cggaagatag aggaagccaa agcggtcgta aaagtctccg agcccgggga tgaacacctg
      301 cctgatatca gacttttgcg cctcaagctt tccggcaatg agttgcgccg cattgcggga
      361 caaggtggag ttttcagccg aaacccgcac gaccatcagg gatgcgacgg cgggaaaatc
      421 cgcctggtag cgcgcctggg aggactgggt tgcccggtca atcgggattt ctacaggacg
      481 gctgaaatca aaacgcgggc cgagtaccgc aagggttgcc gcggcagtca tcaggaaagc
      541 cgcgatcagc agaaccggca tgggcctggc aatgcaaaac cgcgccaatc ttgcaaagag
      601 attgggacgc aggcgcgggc gggccggcgg ccgccgtgcc gaccgcgccc gtgtcggaaa
      661 caactctcgc tgttcctccc aatcctgcat agtagatgcc gcccttagcc agttgcagat
      721 tcgcccaatg gcggccaaca taaagcataa ttcgatggaa tgcaggaaga gatcatggcg
      781 tcaaatacag gcaaaaacag cggccctttg catgaatcga cagttgcggt tcacggcggc
      841 gagttcgttg atccggcaac gcgggcatca tcacccaacc tggtgatgtc cgccactttt
      901 gcgccgaccg atgtgacagg tttttcagcc cgggacgaga aggattacga cggcttcgtc
      961 tatgggaggg tttccagccc cacggtgcgc cagcttgagc agaagcttgc ggctcttgag
     1021 ggcgccgaag cagcgctcgc ctatgcctct ggcgttgccg ccagccatgc gctgattgcc
     1081 gggcgcctga gcaagggtga ccacctgctg atgcccgatt ccaattatgt gggggtggcc
     1141 gaactgacgc gcgataccct gccccgcttc ggcattgaag cgtcctttgt ggacataaca
     1201 gatgcaaaag ctgtggcggc ggaaatcagg ccgaataccc gcatgctttg gcttgaaaca
     1261 ccgagcaatc ccaccatgca gattactgac atcaaggcat tggcggcact tgcccatgaa
     1321 aagggggtgc gcgacgtggt ggttgactca acctacgcta cgcccattgc cacgaaaccc
     1381 ttgagccttg gcgcggattt cgtcattcac tcactcacca aatatatcgg cggacatggc
     1441 gatgcgatgg ggggcgcggt gcttggccgc aagacggatc tcgatgccct caaccttgaa
     1501 gcaaca
//
LOCUS       DAORVN010000621         1505 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_101714_length_1505_cov_3.593793, whole genome shotgun
            sequence.
ACCESSION   DAORVN010000621 DAORVN010000000
VERSION     DAORVN010000621.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1505
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101714_length_1505_cov_3.593793"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            165..770
                     /locus_tag="PKV23_10940"
     CDS             165..770
                     /locus_tag="PKV23_10940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX85995.1"
                     /translation="MPRRKLPSAANNHLRRAIAGAAARLMAEEGIGDYGAAKRKAARS
                     LGASDGEALPTNEEIETELRAWQSLYQEDEQRERVRDLRTTALEIMQLLAEFHPYLTG
                     GALDGTAGRYSAVELDLYADSSKDVEIALLSHGISYETMDNRRAGVDAQLRLEWNDYP
                     VLIAVYPPVAERQQPKSPHGSRGRQRVRAEAVIELLRETPE"
     gene            767..1312
                     /locus_tag="PKV23_10945"
     CDS             767..1312
                     /locus_tag="PKV23_10945"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HQX85996.1"
                     /translation="MNRPLVWVAIAGTIVFAALTGYRLTTIGARPEKMAAPVTAPGLS
                     VFELTLKDLNGKTQNLAQWRGKVLIVNYWATWCHPCREEMPGFSRLQDKYRDKGIQFV
                     GISIDTVDKIIEFQKTTPVNYPLLIGDISIMESSAKLGNTRQALPFTAVFDREGRLSA
                     TKLGRLSEPELEGQLKELISR"
     gene            1351..>1505
                     /locus_tag="PKV23_10950"
     CDS             1351..>1505
                     /locus_tag="PKV23_10950"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012112223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HQX85997.1"
                     /translation="MTKQTRTKANTSRGSNHAVLVLHGPNLNLLGSREPDIYGRTTLA
                     SIHTAMEA"
BASE COUNT          343 a          464 c          430 g          268 t
ORIGIN      
        1 gtcccgcggc acgtgcgagc acagcaatgc cacccatgaa ggtgccgcag atgccgagga
       61 tgtggatatg catgtcgcgc tctttggctt gaatgatgga ataatgggac ggattctatc
      121 ccgccaggga ataccgcccg ccagccggat tttcccacct cgccatgcca cgccgcaaac
      181 ttccctctgc cgccaacaat cacctgcgcc gcgccattgc aggcgccgcg gctcgcctga
      241 tggcggaaga aggcatcggc gactacgggg cggcgaaacg caaggcagct cgaagcctgg
      301 gcgcttccga cggggaggcg ctgcccacca acgaagaaat cgagaccgaa ctgcgcgcct
      361 ggcagtcgct ttaccaggaa gacgaacagc gtgaacgcgt gcgtgacttg cgcacgacag
      421 cgctcgaaat catgcaattg ctggccgagt tccaccccta tctcaccggc ggggcgctgg
      481 acggaaccgc cggtcgctac tccgccgtgg agctcgacct ttacgccgac agcagcaaag
      541 atgtagagat tgcccttcta tctcacggca tctcctacga aaccatggac aatcgccgtg
      601 ctggcgtcga tgcccaattg cggctggaat ggaatgacta tcccgtgctt atcgcggttt
      661 acccacccgt tgccgaaagg caacagccga aaagtccgca tgggagtcgt ggacgccaac
      721 gggtccgcgc cgaggcggtc atcgaactgt tgcgggaaac cccggaatga atcgccccct
      781 agtctgggta gccattgctg gcaccatcgt gtttgccgcg ttgactggct atcggctgac
      841 cacgatcggc gcccgccctg aaaaaatggc ggctccggtg acggccccag gactctcggt
      901 ctttgaactg acgctgaagg acttgaacgg gaaaacgcag aacctcgccc aatggcgcgg
      961 caaggtcctg atcgtcaatt actgggcgac ctggtgccat ccctgtcgcg aggaaatgcc
     1021 gggcttttcc cggctgcagg acaagtatcg tgacaaaggc attcaatttg taggtataag
     1081 catcgatact gtcgacaaaa tcatcgaatt tcagaaaacg accccagtga actacccctt
     1141 gctgatcggt gacatcagca tcatggaaag tagcgccaaa ctgggcaaca cccgccaagc
     1201 attgcctttc accgctgtat tcgaccgcga aggccggctg tccgcaacca agctgggtcg
     1261 cctgtcggag cctgagttgg aagggcaact gaaagaactg atttcacggt agctggacaa
     1321 actgcggtca tttgcggcaa acttgcgcct atgacgaagc aaacacgcac caaagcaaat
     1381 accagccgag ggtcgaacca tgccgttctg gtgctccacg gcccgaatct gaacctgctg
     1441 ggcagtcgtg agccggacat ttacgggcgc accacactgg ccagcatcca caccgccatg
     1501 gaagc
//
LOCUS       DAORVN010000622         1505 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_101767_length_1505_cov_2.409655, whole genome shotgun
            sequence.
ACCESSION   DAORVN010000622 DAORVN010000000
VERSION     DAORVN010000622.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1505
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101767_length_1505_cov_2.409655"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="PKV23_10955"
     CDS             complement(<1..362)
                     /locus_tag="PKV23_10955"
                     /inference="COORDINATES: protein motif:HMM:NF013487.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HQX85998.1"
                     /translation="MKAIASFVLAAAAVTLAAPASAQFAKPEDAVKYRKSAMTIMATH
                     FGRVAAMANGRVPFDAKVAADNAAVAESMSKLPWAAFVEGSDKGDTRAKPEIWSDAVK
                     FKDANEKMQGEMTKLSAAA"
     gene            complement(470..>1505)
                     /locus_tag="PKV23_10960"
     CDS             complement(470..>1505)
                     /locus_tag="PKV23_10960"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HQX85999.1"
                     /translation="PPKSKTRLNGAFSPRDAGNPVFGETPAVFQAPTARAARPRVARE
                     RFAQSKSIGDPLKIHLLLRRAPWHLAATLLLTSATAVSAQGNAVKGQYLANAAGCIGC
                     HTVTKAGATPYAGGRGLETPFGTFYGPNITPDKETGVGTWSEADFKRAIRQGERRDGS
                     HYFPAFPYPSFTGMSDTDIQDLWAFLKTVPPAKQANREHELRFPYSLRFLVGGWKLMF
                     FTPGPSPQATVVASPVARGAYLVGTLGHCGECHTPRNFLGGPDRNELLGGARIPEGKV
                     PNLTPTRLKKWSDADLKEYLMSGTAPSGEIAVEPMSEVITNTTSKLTADDIAAVIAYL
                     RSLPPRDEPK"
BASE COUNT          295 a          422 c          458 g          330 t
ORIGIN      
        1 gcggcagcgg aaagcttggt catctcgcct tgcatcttct cgttggcgtc cttgaacttg
       61 acggcgtcag accagatttc aggcttggcg cgggtgtctc ctttgtcgct gccctccaca
      121 aaagcagccc aaggcagttt tgacatggat tctgcaacag ctgcgttgtc agccgccact
      181 ttggcatcaa agggaacacg cccgttcgcc atggcagcca cacgtccgaa atgggtggcc
      241 atgattgtca tcgccgactt gcggtatttg acggcatctt ccggcttcgc aaactgcgcg
      301 gaagcgggag cagccaaggt gacagcggcg gctgcgagta cgaacgaggc aattgctttc
      361 atggtttatc cttttttgag ttggggttgc taatagaggg gttgagttcg cttagttaag
      421 aaaagttctt tgattttttg ccaccctagg gaaaatactt aaggaatcgt tacttcggct
      481 cgtcgcgagg gggcaaagat cttagataag cgatcacagc agcaatgtcg tctgcggtta
      541 atttgctcgt ggtgttggta atcacctcac tcatgggttc caccgcaatc tcgccactgg
      601 gcgcagtgcc actcattaaa tattccttga ggtccgcgtc gctccacttc ttcaagcgtg
      661 taggcgtcag gttcgggacc ttgccttccg gaatcctggc gccccccaac aattcgttcc
      721 ggtcagggcc gccgaggaaa ttgcgtgggg tatggcactc gccacaatga cccagagtcc
      781 cgaccaagta ggcgccacgg gcaaccgggc tggccacgac cgtggcctgc ggggaaggcc
      841 ccggcgtgaa aaacatcaac ttccacccgc ccacaagaaa gcgcaggctg tacggaaaac
      901 gcagttcatg ctcgcgattg gcctgtttgg ctggcggtac ggtcttcaga aacgcccaga
      961 ggtcctggat atcggtatcc gacatgcccg tgaaggacgg gtacgggaag gccgggaagt
     1021 agtgcgagcc atccctgcgc tcaccctggc ggatcgcgcg cttgaaatcg gcctcggacc
     1081 aagtgccgac gcccgtttcc ttgtctggcg tgatgttggg gccgtagaaa gtcccgaatg
     1141 gcgtttcgag cccacgaccg cccgcatagg gtgttgcgcc agccttggtt accgtgtggc
     1201 acccgatgca tccggcggca ttggccaggt attgcccttt aactgcgttg ccctgtgcgc
     1261 tcacagcagt ggcgctggtg agcagcagtg tggcggccag gtgccaaggg gctcgacgga
     1321 gcaaaaggtg aatcttcaaa gggtctccga tggattttga ttgggcaaac cgctcgcggg
     1381 cgacacgagg tcgcgccgca cgagcagttg gagcctgaaa caccgctgga gtttcgccga
     1441 aaaccgggtt tcctgcatcc cgtggcgaga aggcgccatt cagccgtgtc ttgctctttg
     1501 gaggc
//
LOCUS       DAORVN010000623         1503 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_101976_length_1503_cov_3.091851, whole genome shotgun
            sequence.
ACCESSION   DAORVN010000623 DAORVN010000000
VERSION     DAORVN010000623.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1503)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1503)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1503
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101976_length_1503_cov_3.091851"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1503
                     /gene="tkt"
                     /locus_tag="PKV23_10965"
     CDS             <1..>1503
                     /gene="tkt"
                     /locus_tag="PKV23_10965"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019172862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HQX86000.1"
                     /translation="AVAAANSGHSGMPMGMADVATVLFEKHLKFDPKAPRWPDRDRFI
                     LSAGHGSMLIYALLHLTGYADMTLAQVKNFRQWGAITAGHPEFGHADGVETTTGPLGQ
                     GIANAVGFAMAEESLRARWGAKVMDHYTYVIAGDGCLMEGISQEAITLAGHLKLSHLI
                     VLWDDNGISIDGKVSMSDSTDQLARFASANWNVSRVDGHNPSEVAAALQAAQNSDKPT
                     MIACKTIIGFGAPTKQGTSATHGSPLGAEEIAGARKALGWDYGPFVVPDEILNAWRAV
                     GSRGTSLRKEWEQRLAASAHKAEFNRITSGALPANFDEVVAAYKKELAKNPPWIATRN
                     SSQNALDVINAAVPETIGGSADLTGSNNTKSKDMKALTATDYGARYVYYGIREHGMAA
                     AMNGMALHGGVIPYGGTFLTFTDYCRPSIRLSALMGKRVIYVMTHDSIGLGEDGPTHQ
                     PVEHLAALRVIPNLLTFRPCDAVETLECWQLALQTAASPSVLALTRQKLKP"
BASE COUNT          299 a          515 c          412 g          277 t
ORIGIN      
        1 cgccgtcgcc gccgccaatt ccggccattc cggcatgccg atgggcatgg ccgatgtggc
       61 gaccgtgctt ttcgaaaagc acctgaagtt tgatcccaaa gcgccccgct ggcccgaccg
      121 cgaccgcttt atcctgtcgg cgggccatgg ctcgatgctg atctatgcgc tgctgcacct
      181 gacgggttat gccgatatga ctttggcgca ggtgaaaaac ttccgccaat ggggcgcaat
      241 caccgcaggg caccccgaat ttggccatgc tgacggggtc gaaaccacga ccggcccgct
      301 gggtcagggc atcgccaatg ccgtcggttt tgccatggcc gaggaatcct tgcgcgcccg
      361 ctggggggcc aaggtcatgg atcactacac ctatgtgatc gcaggcgacg gctgcctgat
      421 ggaaggcatt tcccaggaag ccattaccct ggcgggccac ctcaagctca gccacctcat
      481 cgtgctctgg gatgacaatg gcatctcgat cgatggcaag gtgagcatgt ccgattcgac
      541 cgatcagctc gcccgattcg cctccgccaa ctggaatgtg agccgcgtcg acggccacaa
      601 cccttcggaa gtggcagcgg ccctccaggc cgcgcaaaat tccgacaagc ccaccatgat
      661 cgcctgcaaa accatcatcg gttttggcgc accaaccaag cagggcacat ccgcgaccca
      721 tggttcgccg ctgggtgctg aagaaatcgc cggcgcccgc aaggctctcg gctgggacta
      781 cggccccttc gtcgtgcccg atgaaatcct caatgcctgg cgcgccgttg gctcacgcgg
      841 cacttcgctg cgcaaggagt gggaacagcg ccttgcagcc tcagcgcaca aggctgagtt
      901 caaccgcatc acctccggcg ccctgcctgc caatttcgat gaggtcgttg ccgcctataa
      961 aaaggaactg gcaaaaaatc cgccatggat cgccacccgc aattcctcgc agaatgccct
     1021 tgatgtgatc aacgccgccg ttcccgaaac catcggtggc tcggccgatc tcaccggatc
     1081 caacaataca aaatccaagg acatgaaggc cctgactgcc accgactatg gcgcgcgcta
     1141 cgtctattat ggcattcgcg aacatggcat ggccgccgcc atgaacggca tggcgctgca
     1201 cggcggcgtc atcccctatg gcggcacctt cttgaccttc accgattact gccgtcccag
     1261 cattcgcctc tccgccctca tgggcaagcg cgtgatctat gtgatgaccc acgactccat
     1321 cggccttggt gaagatggcc ccacccatca gcccgtcgaa catctcgccg cgctgcgtgt
     1381 catcccaaat cttctgactt tccgcccctg cgatgcggtt gaaacgctgg agtgctggca
     1441 actcgcgctg caaaccgccg caagccccag cgtgctggcc ctcacccggc aaaaactgaa
     1501 gcc
//
LOCUS       DAORVN010000624         1501 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate AMR_MDS_5238
            NODE_102297_length_1501_cov_1.733057, whole genome shotgun
            sequence.
ACCESSION   DAORVN010000624 DAORVN010000000
VERSION     DAORVN010000624.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867455
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (wastewater metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:05:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,182
            Genes (coding)                    :: 2,164
            CDSs (with protein)               :: 2,164
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 5 of 18
            Pseudo Genes (incomplete)         :: 10 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102297_length_1501_cov_1.733057"
                     /isolate="AMR_MDS_5238"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /locus_tag="PKV23_10970"
     CDS             <1..375
                     /locus_tag="PKV23_10970"
                     /inference="COORDINATES: protein motif:HMM:NF019262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX86001.1"
                     /translation="KPIANVQQAVVYTDIVQPILQSKCYSCHGPAKQKGKLRLDEPPF
                     IDKGGEEGKVLIAGNAGESEMIKRLLLPLDNKDHMPPKQKSQLTKAEIELLSWWVASG
                     APWRPRGTALKGQNRVVDSFKS"
     gene            complement(372..599)
                     /locus_tag="PKV23_10975"
     CDS             complement(372..599)
                     /locus_tag="PKV23_10975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQX86002.1"
                     /translation="MGSSTDILQDARALLLNTTLYREFQAEKEEILKHKWIESEKVGY
                     DIGFERALVEWITNHRAAWRRARQQSPGDGC"
     gene            976..>1501
                     /locus_tag="PKV23_10980"
     CDS             976..>1501
                     /locus_tag="PKV23_10980"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: protein motif:HMM:NF015299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HQX86003.1"
                     /translation="MAQQQTLKQAVSYAGIGLHSGNRVNLTFVPAAPNSGIRFRRVDL
                     EEKPELDARTELVVDTQRSTTLGKGAVRIHTVEHVLAALAGAGVTNAVVELDANEPPI
                     GDGSSRDFVRLLHEAGLQPQPDTVEPLNLSSPIELQIGETQMAAFPHEGFKLTCTSAD
                     KGGRFTQFYSLEVTP"
BASE COUNT          342 a          443 c          448 g          268 t
ORIGIN      
        1 aagccaattg ccaatgttca gcaggcggtt gtgtatacag atattgtgca gccaattctg
       61 caatcaaaat gttatagttg tcatggccct gcaaaacaga aaggaaaatt aagattagac
      121 gaacctcctt ttattgacaa aggtggcgaa gaaggtaaag tattaattgc tggcaatgca
      181 ggcgaaagcg aaatgatcaa gcgattgctg cttccgttag ataacaaaga ccacatgccg
      241 ccaaagcaaa aatcacagct tactaaagct gaaattgaat tactcagctg gtgggtggca
      301 tcaggagctc cttggcggcc tcgcgggacc gcattgaagg ggcagaaccg ggtggtggac
      361 agcttcaagt cctagcaacc gtctcccggg gactgctggc gcgcccgacg ccaggcagcg
      421 cggtgattgg taatccattc gaccaacgcc cgctcaaagc cgatgtcgta acccaccttc
      481 tcggattcaa tccacttgtg tttgaggatc tcctccttct cggcctgaaa ttcgcggtaa
      541 agcgtggtat tcaacaggag cgcccgggcg tcttgcagga tgtcagtgga ggaacccatg
      601 gtccgaatcg aatttgtgac cggaacttaa cgacggtcac ctcgctgtaa atcagaatgt
      661 ggccaggcgg ggcggaaagg gcacatcgta acatggcaac ccgatgccgg gagccgggga
      721 tgcgatgaat gacagtcgcg cggcggcggc acccccggcc cggactgccc gagggaaaaa
      781 ccaacgacag acattggcgg aacacgcccg aacggaacgg agcaatcgac ctccgtcgtg
      841 ccccggtctg ggcactgcga agggtccatc ggccccggaa cgatccgtcc gggacccgaa
      901 tcaaccccta agactccgca aggtatcctg ccgcccgcct tgcctcaccc ttcgtcgttt
      961 ggctacaaac cggcggtggc gcaacagcaa acgctcaagc aggcggtctc gtacgcgggt
     1021 atcgggcttc attcggggaa ccgggtgaac ctgaccttcg tgccggccgc gcccaattcc
     1081 gggatccggt tccgccgggt ggatctggag gaaaagccgg aactggacgc ccgcaccgaa
     1141 ctcgtggtgg acacccagcg gtcaacgacc ctcggaaagg gcgcggtccg catccacacc
     1201 gtggagcacg tgctcgccgc actggccgga gcgggggtga ccaatgccgt cgtcgaactg
     1261 gatgccaatg agccgcccat cggggacggc agctcgcgcg attttgtccg tctgttgcat
     1321 gaagccggcc tgcagccgca gccggacacc gtggagccgc tgaatctcag ctcgcccatc
     1381 gagctccaga ttggggagac gcagatggcg gcctttccgc acgaaggctt caagctcacc
     1441 tgcaccagcg ccgacaaggg cggccggttc acccagttct actcgctgga ggttactccg
     1501 g
//
